LOCUS       DASUPW010000001         5536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_138658, whole genome shotgun sequence.
ACCESSION   DASUPW010000001 DASUPW010000000
VERSION     DASUPW010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138658"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2413
                     /locus_tag="VFY44_00005"
     CDS             <1..2413
                     /locus_tag="VFY44_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5908840.1"
                     /translation="REPLGSEPDPLPGGFDTSLAVRALQAAGTGVCIADARRPMLPLV
                     WANEAFSDRTGLPLDEIAGGSCRMLQWADTDSAGLGDLLQELREGGSPVVELKHRRRR
                     HGGTSRLSLSPVTDAEGVLTHLIGVHEGAGTAADTQPAPAVMNHDALTGLPNRVVLRD
                     RLGQALGRLRRREGTVGVLFLDVDGFKQINDRYGHGAGDEFLREVPERLRSALRADDT
                     VARLGGDEFVILLEDCGGEVGALQVAERVLQAFEKPFSLGDETLALRVSMGIAVTETS
                     DESADQMLTNADVAMYRAKHAGGDGLAVYDTTLRARANERLGLARDLRAAIGTDQLSL
                     HYQPIVDLGTERVISVEALVRWDHPVRGRVAPDEFVRLAEETGIIVELGRWVLERACT
                     EFAAALDEAGDRRIELAVNLSPRQLADLGLPALLRDVMARTGLGPGRVALEITETALT
                     QESDSPADRLWALKQEGVRIVLDDFGSGYSSLGHLRRFPIDAIKIDRSFVDGLGTQSR
                     DAAIVGAILPMARALDIEVVAEGVESETKLAHLYALGCRHAQGYLFARPAPMAEIAPL
                     VRRAGSTAADRVTHPELSAHQSRFKAALAAGDAEEAGKAIRAALAAGFDGLAVQAQVI
                     GPALHYIGDEWELGRMGVADEHLATAIAERSLAMVFEAMQAGTIEKRGRVILAAVEGE
                     HHVLGLRMTSDALSAAGFDVVYLGADVPEDSLLKAVARHNPQAVCLTATVPAGARAIV
                     AAGRRLISRHPDLRVVAGGPAVPTSALRRSGVHVAESVEEAVSMVERGATTPVVGANG
                     LAAAG"
     gene            2444..2764
                     /locus_tag="VFY44_00010"
     CDS             2444..2764
                     /locus_tag="VFY44_00010"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX5908841.1"
                     /translation="MYVSMSRLRVPSQRAPELVAAFQRRAHLVDDADGFVDLEVWQSD
                     RDSDEETSELIMVSRWRERECFTAYMRSAEHKQSHDRIDPGLDDAIKLERLEHLSTYE
                     VVAR"
     gene            2848..3171
                     /locus_tag="VFY44_00015"
     CDS             2848..3171
                     /locus_tag="VFY44_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908842.1"
                     /translation="MARDICTRYYEQYPDEHDRYGARGAAWCVYDNQHIVHWAALDLA
                     QYADLDGQLAWLGRLLDARDFPMDRFVRNLEIASEVVAARDGDTAALADRLARAAREF
                     DPPAS"
     gene            complement(3164..4093)
                     /locus_tag="VFY44_00020"
     CDS             complement(3164..4093)
                     /locus_tag="VFY44_00020"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF024801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX5908843.1"
                     /translation="MASSTAPSGASFEREQTTLRDWVTADGSSGFAAEPGRYHLYVSL
                     ACPWAHRTIIVRALKGLEDAVTMSVLDPIRDERSWAFRDVPGAEPDPVNDFAFLRQAY
                     EATDPSWSAHVSVPVLWDRQEGRIVNQESADIAVMLNEAWDEWATHDIDLYPAEHRDE
                     IDELNAWIYATVNNGVYRAGFAYAQSSYEDAVVPLFESLDSLEQRLTDRRYLVGDGHT
                     LADWRLFTTLLRFDPVYHGHFKCNVRRIVDYPALWGYLRDLLSVPGVRQTVNMDHIKR
                     HYYMTHPQINPSRVVPVGPELDYDAPHGRESLS"
     gene            4080..4418
                     /locus_tag="VFY44_00025"
     CDS             4080..4418
                     /locus_tag="VFY44_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908844.1"
                     /translation="MELAMVRLPSPVHDPFTPRSPALHRRAGVRVDALAQMNERAIEN
                     GWHYGQLTFVALVTACLLTFVFEQAAGGFGGLTGMIVWSFAAGFGLEFVSAFWTGRAR
                     GTGRRRSAHR"
     gene            4475..5098
                     /locus_tag="VFY44_00030"
     CDS             4475..5098
                     /locus_tag="VFY44_00030"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX5908845.1"
                     /translation="MQVRRSDILKASPPMFESRALDRLTRVHPIVPPLIFIPAIAVTF
                     ALGQEGIAPLPVIAWLVAGYVTWSLTEYWLHRIVFHFEPTEGIGARLHWMIHGVHHDH
                     PNDPMRLVMPPSASVPLALAFYGLFHLVLGGQQADLVACGFLAGYLVYDMTHYYLHHR
                     QARTRFGKLFYELHMRHHFQDDTRSFGVSVPYWDYVFGTAARRKRRR"
     gene            5155..>5536
                     /locus_tag="VFY44_00035"
     CDS             5155..>5536
                     /locus_tag="VFY44_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908846.1"
                     /translation="MTRLGHADMDAVLDFVGEARSFTTLPAFREGILPGLRRLVPCDL
                     VGYNEVDTRSGRSLVLLDEPERQLEDIEATLPRLAHQHPLIKLQASGRHETAAISDFL
                     SARQFHRLELYDEIYRKLGAEDQIA"
BASE COUNT          757 a         2028 c         1969 g          782 t
ORIGIN      
        1 ccgagagcca ctcggctctg agccggatcc ccttcccggc gggttcgaca cgtcgctggc
       61 cgtccgcgcg cttcaggccg ccggcaccgg cgtgtgcatc gccgacgccc gccgccccat
      121 gctcccgctg gtgtgggcca acgaggcctt cagcgaccgc accggcctgc ccctggacga
      181 gatcgcgggc gggagctgcc ggatgctcca gtgggccgac accgactccg ccggcctcgg
      241 cgacctgctg caggagctcc gcgagggcgg gtcgcccgtg gtcgagctca agcaccggcg
      301 ccgccgccac ggcggcacca gccgcctctc cctctccccc gtcaccgacg ccgagggcgt
      361 gctgacccac ctgatcggcg tccacgaggg cgccgggacc gcggccgaca cccagcccgc
      421 gcccgcggtg atgaaccacg acgcgctcac cggcctcccc aaccgcgtgg tcctgcgcga
      481 ccgcctgggc caggcgctgg gccggctccg gcgccgcgag ggcacggtcg gcgtgctgtt
      541 cctggacgtg gacggcttca agcagatcaa cgaccgctac gggcacggcg caggcgacga
      601 gttcctgcgc gaggtcccgg agcggctgcg ctccgcgctg cgcgccgacg acaccgtggc
      661 ccgcctgggc ggcgacgagt tcgtgatcct gctggaggac tgcggcggcg aggtgggcgc
      721 cctgcaggtg gccgagcgcg tgctccaggc cttcgagaag ccgttctcgc tgggcgacga
      781 gacgctggcg ctccgggtct cgatgggcat cgcggtgacc gagacctccg acgagagcgc
      841 cgaccagatg ctgaccaacg ccgacgtggc catgtaccgc gccaagcacg ccggcggcga
      901 cggcctggcg gtctacgaca ccaccctgcg cgcccgcgcg aacgagcgcc tggggctggc
      961 gcgcgacctg cgcgcggcca tcgggaccga tcagctctcg ctgcactacc agccgatcgt
     1021 ggacctgggc accgagcgcg tgatctcggt cgaggccctg gtgcgctggg accaccccgt
     1081 ccgcggccgg gtcgcccccg acgagttcgt gcgactggcc gaggagaccg ggatcatcgt
     1141 ggagctcggg cgctgggtcc tggagcgggc ctgcaccgag ttcgccgccg ccctggacga
     1201 ggccggcgac cgccggatcg agctggccgt gaacctctcc ccgcgccagc tggccgacct
     1261 gggcctgccg gcgctgctgc gcgacgtcat ggcccgcacc ggcctggggc cgggccgcgt
     1321 ggccctggag atcaccgaga ccgcgctgac ccaggagtcc gactcgcccg ccgaccgcct
     1381 gtgggcgctc aagcaggagg gcgtgcggat cgtcctggac gacttcggct cgggctactc
     1441 ctcgctcggc cacctcaggc gcttcccgat cgacgcgatc aagatcgacc gctcgttcgt
     1501 ggacgggctg ggcacccagt cccgcgacgc ggccatcgtc ggcgcgatcc tgccgatggc
     1561 ccgggccctg gacatcgagg tcgtggccga gggcgtggag tccgagacca agctggccca
     1621 cctgtacgcg ctcggctgcc gtcacgccca gggctacctg ttcgcgcgcc ccgcccccat
     1681 ggccgagatc gcgccgctgg tgcgccgcgc cgggagcacc gcggccgacc gcgtcaccca
     1741 ccccgagctg agcgcgcacc agtcgcgctt caaggccgcc ctggccgccg gggacgccga
     1801 ggaggccggc aaggccatcc gcgccgcgct ggccgccggg ttcgacggcc tggccgtgca
     1861 ggcccaggtg atcggccccg ccctgcacta catcggcgac gagtgggagc tgggccgcat
     1921 gggcgtggcc gacgagcacc tggccacggc catcgccgag cggtcgctgg ccatggtctt
     1981 cgaggccatg caggccggga cgatcgagaa gcgcggccgc gtgatcctcg ccgccgtcga
     2041 gggcgagcac cacgtcctgg gcctgcgcat gacctccgac gccctgagcg cggccgggtt
     2101 cgacgtggtc tacctgggcg ccgacgtgcc cgaggactcg ctgctgaagg ccgtcgcgcg
     2161 gcacaacccc caggccgtct gcctgaccgc cacggtgccc gccggcgccc gggcgatcgt
     2221 ggccgccggg cggaggctga tctcgcgcca ccccgacctc cgagtggtgg ccggggggcc
     2281 cgcggtgccc acgtccgccc tccgccgctc gggcgtgcac gtggccgaga gcgtcgaaga
     2341 ggcggtcagc atggtggagc ggggcgcgac aaccccggta gttggcgcaa acggcctcgc
     2401 ggccgctggc tagaaacggg tactcgggag tacattcgcg ccgatgtacg tgtcgatgtc
     2461 acgactgcgc gtgcccagcc agagggcgcc cgagctggtg gcggcgttcc agcgccgcgc
     2521 ccatctggtc gacgacgccg acggcttcgt ggacctggag gtctggcagt ccgaccgcga
     2581 ctccgacgag gagaccagcg agctgatcat ggtcagccgc tggcgcgagc gcgagtgctt
     2641 caccgcatac atgcgcagcg ccgagcacaa gcagtcccac gaccgcatcg acccgggcct
     2701 ggacgacgcg atcaagctcg agcgcctgga gcacctgagc acctacgagg tggtggcgcg
     2761 ctgagccccg gcgagcaccc cgacgaggcc caccccccgc ccggacgggc cgagctcccc
     2821 gacgggagcg agctcgagct ggagccggtg gcgcgcgaca tctgcacgcg ctactacgag
     2881 cagtaccccg acgagcacga ccgctacggc gcccgcggcg ccgcgtggtg cgtctacgac
     2941 aaccagcaca tcgtccactg ggcggcgctg gacctggcgc agtacgccga cctggacggc
     3001 cagctggcct ggctgggccg cctgctggac gcgcgcgact tcccgatgga ccggttcgtc
     3061 cgcaacctgg agatcgcctc cgaggtcgtg gccgcccggg acggggacac cgccgcgctg
     3121 gccgaccgcc tggcgcgggc ggcgcgcgag ttcgacccgc ccgctagctg agcgactcgc
     3181 gcccgtgggg cgcgtcgtag tccagctcgg gccccaccgg gaccacccgg ctgggattga
     3241 tctgcggatg ggtcatgtag tagtggcgct tgatgtggtc catgttcacc gtctgccgga
     3301 cgccgggcac cgacagcagg tcgcgcaggt agccccacag cgccgggtag tcgacgatcc
     3361 ggcgcacgtt gcacttgaag tggccgtggt agaccgggtc gaagcgcagc agcgtggtga
     3421 acaggcgcca gtcggccagc gtgtggccgt cgcccaccag gtagcggcgg tcggtcagcc
     3481 gctgctcgag tgagtccagc gactcgaaga gcggcaccac cgcgtcctcg tagctggact
     3541 gggcgtaggc gaagccggcg cggtagacgc cgttgttgac cgtcgcgtag atccaggcgt
     3601 tcagctcgtc gatctcgtcg cggtgctcgg ccgggtacag gtcgatgtcg tgggtggccc
     3661 actcgtccca ggcctcgttg agcatcaccg cgatgtcggc cgactcctgg ttgacgatcc
     3721 ggccctcctg gcggtcccac agcaccggga cgctgacgtg ggcgctccag gaggggtcgg
     3781 tggcctcgta ggcctggcgc aggaaggcga agtcgttcac cgggtcgggc tcggcgccgg
     3841 ggacgtcgcg gaaggcccag ctgcgctcgt cccggatcgg gtcgagcacg gacatcgtga
     3901 ccgcgtcctc caggcccttg agcgcgcgga cgatgatcgt gcggtgggcc cagggacagg
     3961 ccagcgacac gtacaggtgg tagcgcccgg gctcggccgc gaagcccgac gagccgtcgg
     4021 ccgtgaccca gtcgcggagc gtggtctgct cgcgctcgaa gctggcgccg gagggtgcgg
     4081 tggagctggc catggtccgg ctcccctccc cggttcacga tcccttcaca ccccgatcac
     4141 cggcgcttca ccgccgcgca ggcgtgcggg tcgatgcttt ggcgcaaatg aacgagagag
     4201 cgatagagaa cggctggcac tacggtcagc tcacattcgt ggcactggtg acggcctgcc
     4261 tgctgacgtt cgtgttcgaa caggcggccg gcgggttcgg cggcctcacg ggaatgatcg
     4321 tgtggtcgtt cgccgcgggg ttcgggctgg agttcgtctc ggccttctgg accgggcgcg
     4381 cccggggcac cggacgacgc cgctcagctc accgctgagg gtgcggttat ccacaccagc
     4441 gcacccccag gaggggtgct actttggcct gacgatgcag gtgcgtcgtt cggacatcct
     4501 caaggcctct ccgccgatgt tcgagtcgcg tgcgctggac cgcctgacgc gggtccaccc
     4561 catcgtcccg ccgctgatct tcatcccggc gatcgccgtc accttcgcgc tgggtcagga
     4621 gggcatcgcc ccgctgcccg tgatcgcgtg gctggtcgcg ggatacgtga cctggtcgct
     4681 gaccgagtac tggctgcacc ggatcgtctt ccacttcgag ccgacggagg gcatcggcgc
     4741 gcggctgcac tggatgatcc acggtgtcca ccacgaccac cccaacgacc cgatgcggct
     4801 ggtcatgccg ccgtcggcca gcgtgccgct ggcgctggcc ttctacgggc tcttccacct
     4861 ggtcctcggc ggccagcagg ccgacctggt ggcctgcggg ttcctggcgg gctacctggt
     4921 ctacgacatg acgcactact acctgcacca ccgccaggcc cgcacgcggt tcgggaagct
     4981 cttctacgag ctgcacatgc gccaccactt ccaggacgac acgcgcagct tcggcgtcag
     5041 cgtgccctac tgggactacg tgttcggcac cgccgcacgc cgcaagcgcc gccgctagtc
     5101 ctggcgcgga tcctcctcct ggcgtagcgt ggtaccaccc gacaggggga cgtcttgacc
     5161 cgcctgggcc acgccgatat ggacgccgtg ctggacttcg tgggcgaagc gcgctcgttc
     5221 accacgctgc ccgccttccg cgagggcatc ctgcccgggc tccggcggct cgtgccctgc
     5281 gacctggtgg gctacaacga ggtggacacc cgcagcgggc ggtcgctggt gctgctggac
     5341 gagcccgagc gccagctcga ggacatcgag gccacgctgc cccggctcgc gcaccagcac
     5401 cccctgatca agctccaggc ctcgggcagg cacgagaccg cggccatctc cgacttcctg
     5461 tcggcgcgcc agttccaccg gctcgagctc tacgacgaga tctaccgcaa gctgggggcg
     5521 gaggatcaga tcgcct
//
LOCUS       DASUPW010000002         2437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1524074, whole genome shotgun sequence.
ACCESSION   DASUPW010000002 DASUPW010000000
VERSION     DASUPW010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524074"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2437
                     /locus_tag="VFY44_00040"
     CDS             <1..>2437
                     /locus_tag="VFY44_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908847.1"
                     /translation="KPVTPVTLASPDQIDRQRQARRRPEPEPEPQPEPQPQPRPGAQP
                     KPAAQPAPPVTLAAPDQIDRRRQARRRPEPEATPQPRPAARPEPAAAQPNAEPEPAAP
                     KPSDPEQVRSEAERYANALDQIEADDGALRMGTSQAMQLGDDPRVVRQARQIREDEAA
                     ARQAEAERIRRHEAKVAEDARVLRESRSYMNAQEQIKDNGGALNMGLSRALSYGDDPK
                     VKGFVTEYQRQVEQREREAREQERREQTRRKAETYLNALDRIEDNGGALSMGLSNNLR
                     LADDPEVRRVADRMSERREAAEERERVLDLARTHLDGDPEAPLPEDPAVRDAVRELEQ
                     KRQTRSDAERYANALDRIDDNGGALNMGLSRTLQEFADDNKVRRLAEQLRAERANEYK
                     EEVFGPLDGPSLRDPTLGIAIPFPEHDGTSVGLTFEQECMLKGTPNAALACVQGPKKP
                     PPAAVKLTGKVTGEVSRGNPELADNGKTYENLSVSVDIQAGVQGDGKLPKGVGITLSK
                     YNGETFTYKLRVTSERAEQIANGTVRPPVPIDPDSLRAGESITMDEEAYEGNSQSASY
                     RILKGTLGFQEGRRLSSAVERLPSGRMRILVGDEEFVKNAIELRLGTDDFGVAAGVSK
                     EFANGDLRTVEINPRNESGWDAYQHFVTTGTLPATEGPGVTDTTSVEKASWSSSTTLK
                     LDLKPIHVGGSSTPFAGDVTETHHSDGSVDYALTDRHGGVTYVEQAKKELGDEEYGPS
                     EYTLLLQGADSSLAEALTELHGGDVEAIDGDDQNLRLRFSEADVERLRRDAVEQISHY
                     TNQGDDDEGMTPAE"
BASE COUNT          435 a          906 c          861 g          235 t
ORIGIN      
        1 gaagcccgtc acgcccgtca ccctcgcctc gcccgatcag atcgaccgcc agcgccaggc
       61 ccggcgccgc cccgagcccg agcccgagcc ccagccggag ccccagcccc agccgaggcc
      121 cggggcccag ccgaagcccg cggcccagcc ggctccgccc gtcacccttg ccgccccgga
      181 tcagatcgac cgccggcgcc aggcccggcg ccgccccgag ccggaggcca cgccccagcc
      241 gaggcccgcc gcccggcccg agcccgcggc ggcccagccg aacgccgagc ccgagcccgc
      301 tgcgcccaag ccctccgacc ccgagcaggt gcgctccgag gccgagcggt acgccaacgc
      361 cctggaccag atcgaggccg acgacggtgc cctgcgcatg gggacctccc aggccatgca
      421 gctgggcgac gacccccggg tcgtcaggca ggccaggcag atccgcgagg acgaggccgc
      481 cgcgcgccag gccgaggccg agcgcatccg ccggcacgag gccaaggtgg ccgaggacgc
      541 ccgtgtgctg cgcgagagcc gctcgtacat gaacgcgcag gagcagatca aggacaacgg
      601 cggcgccctg aacatgggcc tctcccgcgc gctctcgtac ggcgacgatc ccaaggtcaa
      661 gggcttcgtg accgagtacc agcgccaggt cgagcagcgt gagcgcgagg cgcgcgagca
      721 ggagcgccgg gagcagacca ggcgtaaggc cgagacctac ctgaacgccc tggaccggat
      781 cgaggacaac gggggcgcgc tgagcatggg cctgtccaac aacctgcgcc tggccgacga
      841 ccccgaggtc aggcgcgtgg ccgaccggat gtccgagcgc cgtgaggcgg ccgaggagcg
      901 cgagcgcgtc ctggacctgg cccgcaccca cctggacggc gaccccgagg cgcccctgcc
      961 cgaggatccc gccgtgcgcg acgccgtgag ggagctcgag cagaagcgcc agacgcgcag
     1021 cgacgccgag cgctacgcca acgcgctgga ccggatcgac gacaacggcg gcgcgctgaa
     1081 catgggcctc tcgcggacgc tccaggagtt cgccgacgac aacaaggtgc ggcggctggc
     1141 cgagcagctg cgcgccgaga gggcgaacga gtacaaggag gaggtcttcg ggccgctcga
     1201 cgggccgtcg ctgcgcgatc ccacgctggg aatcgcgatt cccttccccg agcacgacgg
     1261 gacgtcggtc ggcctcacct tcgagcagga gtgcatgctc aagggcaccc cgaacgcggc
     1321 gctggcctgc gtgcaggggc cgaagaagcc gccgcccgcc gcggtcaagc tgaccgggaa
     1381 ggtcaccggc gaggtcagcc gcggcaaccc cgagctggcc gacaacggca agacgtacga
     1441 gaacctcagc gtgtccgtcg acatccaggc cggcgtgcag ggcgacggca agctgcccaa
     1501 gggcgtcggc atcacgctga gcaagtacaa cggcgagacc ttcacctaca agctgcgcgt
     1561 gacctcggag cgcgccgagc agatcgccaa cggcaccgtc cgcccgcccg tgcccatcga
     1621 tcccgactcg ctccgcgcgg gcgagagcat cacgatggac gaggaggcct acgagggcaa
     1681 ctcgcagagc gccagctaca ggatcctcaa gggcacgctg ggcttccagg aggggcggcg
     1741 cctgagcagc gcggtcgagc ggctgcccag cggccggatg cgcatcctgg tcggtgacga
     1801 ggagttcgtg aagaacgcca tcgagctgcg gctgggcacc gacgacttcg gcgtggccgc
     1861 gggggtgtcc aaggagttcg ccaacggcga tctgcgcacc gttgagatca acccccggaa
     1921 cgagtccggc tgggacgcct accagcactt cgtgaccacg ggcacgctgc ccgccaccga
     1981 ggggcccggg gtgaccgaca ccacctcggt cgagaaggcc agctggtcgt ccagcaccac
     2041 gctcaagctg gacctcaagc cgatccacgt gggcggcagc tcgaccccgt tcgccggcga
     2101 cgtgaccgag acccaccact ccgacggcag cgtggactac gccctgaccg accgccatgg
     2161 cggcgtgacc tacgtcgagc aggccaagaa ggagctgggc gacgaggagt acggcccgtc
     2221 cgagtacacc ctcctgctcc agggggcgga ctcgagcctg gccgaggccc tgaccgagct
     2281 gcacggcggc gacgtcgagg cgatcgacgg cgacgaccag aacctgcgcc tgcgcttcag
     2341 cgaggcggac gtggagcgcc tgcgccggga cgcggtcgag cagatctcgc actacacgaa
     2401 ccagggcgac gacgacgagg gcatgacgcc ggccgag
//
LOCUS       DASUPW010000003         4692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1108499, whole genome shotgun sequence.
ACCESSION   DASUPW010000003 DASUPW010000000
VERSION     DASUPW010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108499"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VFY44_00045"
     CDS             complement(<1..509)
                     /locus_tag="VFY44_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5908848.1"
                     /translation="MARLLMPRYLIPILIALACVGAVTLIDNDGQRAVAQKAQDAQSK
                     KPSVVLIIMDEFPVDQMLRSDGKIDAVRYPNFAALAAGGTWFKNASTTYDSTTRAIPQ
                     VMDGKLARRSSTPDFVGHPDSIYTIFGRRGYRIVSSQEATSVCPPRYCPGARRKRPDI
                     LPLLQNGRRA"
     gene            651..2366
                     /locus_tag="VFY44_00050"
     CDS             651..2366
                     /locus_tag="VFY44_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908849.1"
                     /translation="MTVRRFVAGLSAAALLATAPAALAAPALDRPAEPVVVSGGQAPA
                     IVGVEPGKVVAFAWRGTRWAQIPVQVDERKTIDLRAAYPASVNCSGSLCYRPTSTGPQ
                     LRYADPGTLIGPDPDARVDADDEVALMARDTGPRSPAGTPAPAGVVTGLRQELAVTDP
                     ITHARSYVYLFRSRGGLGQGAGKSYVDYRFRLAAGAYPAAYSFASGPNAEASTVTTPY
                     YRRSFSDRWRESGASITAPGASGVNVLDRNEAQFSPDYCGRTTLTFARGEGAFLVNRT
                     GPVRAIRSVVGANSGPMSERQQVFYDRRTDETTYLRVHPIPGIMSFWDYSAAASGMRY
                     QNSNNPAGVIVDGRPDAVAPGPLRWESVDGPQGAATHVFSWEGTLSPAGFSSFYRDDA
                     SARACEGDADGSFRGASGPHVTSPIGTTDGTSPAERLTASRVTFFDAPGRSRGQARDR
                     EVRYPLTVSRPAPARAAAGRIKLMRVRVTRAGAIRMAVRVNGRGRVSAAVARKGRRRV
                     GTKAAKRTRAAGTVRLNVRLNKAGRRLARARTRGLVVRVKLGFRPSAGKATSRSRAVR
                     VRVRR"
     gene            complement(2370..3128)
                     /locus_tag="VFY44_00055"
     CDS             complement(2370..3128)
                     /locus_tag="VFY44_00055"
                     /inference="COORDINATES: protein motif:HMM:NF015909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908850.1"
                     /translation="MGRLDELDLSLKLSRKEQDERLEAGYDRLEALRLEMGGKLEGPE
                     LGPPVCVLFEGWDASGKGGAIKRLVAPLDQRHVRVAQFAAPTPTEKRHHFLWRFWPVL
                     PGWGGLTIMDRSWYGRVLVERVEGFATADEWRRSYDEIVAFERSMASDGMVIVKFWMH
                     ISDEEQLRRFERREKKPLKRWKLTDEDWRNREKRDAYTDAIEEMLERTDHTDAHWHLV
                     EGDSKRWARVKVLETVIAEMEAGLRDRGTRPPPG"
     gene            3199..>4692
                     /locus_tag="VFY44_00060"
     CDS             3199..>4692
                     /locus_tag="VFY44_00060"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5908851.1"
                     /translation="MSDASHISARNRARLLLAAAVLVIAVSVWGVSAQQRSTADRVFD
                     ETRSAQQMLAAMLDQETGVRGFALTRQDTFLEPYRQGTAAFARALREARRLVHEDDTR
                     RQLEAQAAAARRWRTMADEQLLSLRISPGQRFSVKKAEARKRAFDRFRSLNARLSGDL
                     AAERKRELGRAGAISAGVTILLALLFGGLGYVVIERQARRGRARRAAERQYRETQSEF
                     AETIQIMRNEDEAHTLVKDHLEREIEGARATALVRNNSDDRLVAATPVDPDSPLAAGL
                     MDASPESCLAVRLARPYEQGRDSSPLLPCKLCGKSAPDVTCVPSLVSGEVIGSVLIQS
                     ETPLQPTERERVVDSIGQASPVLANLRNLAIAETRASTDALTGLPNSRSCRDNLKRMV
                     AAAGRTFSPVTAVLLDLDHFKQINDRFGHGAGDDVLAAVGDVLRSTLRDSDFAGRYGG
                     EEFLMLLPDTDHEGGLQAAEKVREAVQALDVSQVDGPVTASMGVASFP"
BASE COUNT          622 a         1745 c         1686 g          639 t
ORIGIN      
        1 gcgcggcggc cgttctggag cagcgggagg atgtcggggc gcttgcgccg tgcgccaggg
       61 cagtagcggg gcgggcagac cgaggtcgct tcctgggagg agacgatccg gtagccgcgg
      121 cggccgaaga tggtgtagat cgaatcgggg tggcccacga agtcgggcgt gctggaccgg
      181 cgcgccagct tgccgtccat gacctgcggg atcgcgcggg tggtggagtc gtaggtggtg
      241 ctggcgttct tgaaccacgt cccgccggcg gcgagggcgg cgaagttggg gtaccggacg
      301 gcgtcgatct tgccgtcgga gcgcagcatc tggtccaccg ggaactcgtc catgatgatc
      361 aggacgaccg agggcttctt ggactgcgcg tcctgcgcct tctgcgccac ggcgcgctgg
      421 ccgtcgttgt cgatcagcgt gaccgcgccg acgcaggcca gggcgatgag gatggggatg
      481 aggtaccggg gcatgagcag tctggccatc gcgaacggaa gcctatggcc tttacgaacg
      541 gatcacccgc ccgtaacggc ggcgtcaccg gtgggtcaca acccgggctg tgaccgaaag
      601 tcgcgccgga gccctcaagt tcgcgccgcc gcgggtcgat gacggtccgg atgaccgttc
      661 gccgcttcgt ggccggcttg tcggcggcgg ccctcctggc cacggcgccg gcggccctcg
      721 ccgcacccgc cctcgaccgg cccgcggagc ccgtggtggt ctcgggcggc caggcgcccg
      781 cgatcgtggg cgtcgagccc ggcaaggtcg tggcgttcgc ctggcgcggc acgcgctggg
      841 cccagatccc ggtgcaggtg gacgagcgca agacgatcga cctccgggcc gcctaccccg
      901 cgtcggtgaa ctgcagcggc tcgctctgtt accggccgac gtccaccggc ccgcagctgc
      961 gctacgccga ccccggcacc ctgatcggcc ccgaccccga cgcccgcgtg gacgccgacg
     1021 acgaggtggc gctgatggct cgggacacgg gcccgcgctc gcccgcggga accccggcac
     1081 cggcgggcgt ggtcaccggc ctgcgccagg agctcgcggt cacggacccg atcacgcacg
     1141 cccgctccta cgtctacctc ttccgctcac gcggcgggct cggccagggg gccggcaagt
     1201 cctacgtgga ctaccgcttc cggctggcgg ccggtgccta cccggcggcg tactcgttcg
     1261 cctcgggccc gaacgcggag gcctccacgg tcacgacgcc ctactaccgc cgctcgttct
     1321 ccgatcgctg gcgcgagtcg ggcgcgtcga tcaccgcccc cggcgccagc ggcgtgaacg
     1381 tgctggatcg caacgaggcc cagttctcgc ccgactactg cggccggacc acgctgacgt
     1441 tcgcgcgcgg ggagggcgcc ttcctggtga accggaccgg cccggtgcgt gccatccggt
     1501 ccgtcgtggg cgccaacagc ggcccgatgt ccgaacgcca gcaggtgttc tacgaccgcc
     1561 gcaccgacga gaccacctac ctgcgcgtgc accccatccc ggggatcatg agcttctggg
     1621 actacagcgc ggccgcctcc gggatgaggt accagaacag caacaacccg gccggcgtga
     1681 tcgtggacgg ccggcccgac gccgtcgcgc ccggcccgct gcggtgggag tcggtcgacg
     1741 gtccccaggg cgcggcgacc cacgtcttct cgtgggaggg cacgctgtcc ccggcgggct
     1801 tcagctcctt ctaccgcgac gacgcctccg ccagggcctg cgagggcgac gccgacgggt
     1861 ccttccgcgg cgccagcggc ccccacgtga catcgcccat cggcaccacc gacgggacca
     1921 gcccggccga gcggctcacc gcgtcgcggg tgaccttctt cgacgcgccc ggccgctcgc
     1981 gcggtcaggc ccgcgaccgc gaggtccgct acccgctgac ggtcagccgc cccgcgccgg
     2041 cgcgtgccgc tgccgggcgg atcaagctga tgcgcgtccg cgtcacgcgc gcgggcgcga
     2101 tcaggatggc ggtccgcgtc aacggccgcg gccgggtcag cgccgcggtg gcccgcaagg
     2161 gcaggcgacg ggtgggcacg aaggccgcga agcgcacgcg tgccgcgggc acggtgcggc
     2221 tgaacgtccg cctgaacaag gccggccggc gtctggcgcg agcgcgcacg cgcggcctgg
     2281 tggtgcgggt caagctcggc ttccggccca gcgccggcaa ggccacgtcc cgcagccgcg
     2341 ccgtccgggt ccgcgtccgg cgctgagcgt cagcccggcg gcggccgcgt gccgcggtcg
     2401 cgcagcccgg cctccatctc ggcgatcacc gtctccagca ccttcacgcg cgcccaccgc
     2461 ttggagtcac cctcgaccag gtgccagtgg gcgtcggtgt ggtcggtgcg ctccagcatc
     2521 tcctcgatcg cgtcggtgta cgcgtcgcgc ttctcgcggt tgcgccagtc ctcgtcggtc
     2581 agcttccacc gcttgagcgg cttcttctcg cggcgttcga accggcgcag ctgctcctcg
     2641 tcggagatgt gcatccagaa cttcactatc accatgccgt cgctggccat cgaccgctcg
     2701 aaggccacga tctcgtcgta ggagcggcgc cattcgtccg cggtggcgaa gccctccacc
     2761 cgctccacca gcacgcgccc gtaccaggac cggtccatga tggtcaggcc gccccagccg
     2821 ggcaggacgg gccagaaccg ccacaggaag tggtggcgct tctccgtcgg ggtgggggcg
     2881 gcgaactgcg cgacgcggac gtggcgctgg tccagcggcg cgaccaggcg cttgatcgcc
     2941 ccgcccttcc cggaggcgtc ccagccctcg aacagcacgc acacgggcgg gcccagctcg
     3001 ggtccctcga gcttgccgcc catctccagg cgcaacgcct caagccggtc gtagcccgcc
     3061 tccaggcgtt cgtcctgctc cttgcgcgag agcttgagcg acaggtcgag ttcgtccagg
     3121 cggcccatgc cgggatgagc gtaaccgtgc ggccccctca aggcatccgc cgggcggtcg
     3181 aaaggaacca cagacgccgt gtccgacgca tcgcacatct ctgcccggaa ccgggcccgc
     3241 ctgctgctgg ccgcggccgt gctggtcatc gccgtgtcgg tctggggggt gtcggcccag
     3301 cagcgcagca ccgccgaccg ggtgttcgac gagacgcggt ccgcacagca gatgctcgcg
     3361 gccatgctcg accaggagac gggcgtgcgc gggttcgcgc tgacccgtca ggacaccttc
     3421 ctggagccct accggcaggg caccgccgcg ttcgcgcggg cgctgcgtga ggcccgccgg
     3481 ctggtccacg aggacgacac ccgccgtcag ctcgaggccc aggcggcggc cgcgcgccgc
     3541 tggcggacca tggccgacga gcagcttctg tcgctgcgga tcagccccgg ccagcgtttc
     3601 agcgtgaaga aggccgaggc ccgcaagcgc gcgttcgacc gcttccgctc cctcaacgcc
     3661 cgcctcagcg gcgacctggc ggcggagcgc aagcgcgagc tcggacgcgc cggcgcgatc
     3721 tcggccggcg tgaccatcct gctggcgctg ctgttcgggg ggctcggcta cgtggtcatc
     3781 gagcgccagg cgcgccgggg ccgcgcccgc cgcgccgccg agcgccagta ccgcgagacc
     3841 cagagcgagt tcgccgagac gatccagatc atgcgcaacg aggacgaggc gcacacgctg
     3901 gtcaaggacc acctcgagcg cgagatcgag ggggcccgcg ccaccgcgct cgtgcgcaac
     3961 aactccgacg accggctggt ggcggccacc ccggtcgacc ccgactcgcc gttggcggcg
     4021 ggcctgatgg acgcgtcccc cgagagctgc ctggcggtcc ggctcgcgcg tccctacgag
     4081 cagggccggg attcctcgcc gctgttgccc tgcaagctgt gcggcaagtc ggccccggac
     4141 gtcacgtgcg tgccctcgct ggtcagcggc gaggtcatcg ggtcggtgct gatccagagc
     4201 gagacgccgc tgcagcccac cgagcgcgag cgcgtggtgg actcgatcgg ccaggcctcg
     4261 ccggtgctgg ccaacctgcg caacctggcc atcgccgaga cccgcgcgtc gaccgacgcg
     4321 ctgaccgggc tgcccaattc gcgctcgtgc cgcgacaacc tcaagcggat ggtggccgcg
     4381 gccggccgga ccttctctcc ggtcaccgcc gtgctgctgg acctggacca cttcaagcag
     4441 atcaacgacc gcttcggcca cggtgcgggc gacgacgtcc tggccgccgt gggcgacgtg
     4501 ttgcgcagca cgctgcgcga cagcgacttc gccggccgct acggcggcga ggagttcctg
     4561 atgctgctgc ccgacaccga ccacgaaggt ggcctgcagg cggccgagaa ggtgcgcgaa
     4621 gcggtccagg cgctggacgt gtcgcaggtc gacgggccgg tcacggccag catgggcgtg
     4681 gcctcgttcc cc
//
LOCUS       DASUPW010000004         4904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1385552, whole genome shotgun sequence.
ACCESSION   DASUPW010000004 DASUPW010000000
VERSION     DASUPW010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385552"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VFY44_00065"
     CDS             <1..630
                     /locus_tag="VFY44_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908852.1"
                     /translation="NRALSEALALLPTTLRRGNSTFVNLRSTLDDLDVLVDESKPATK
                     RLPTLFNRLKLLTAESRPTVRSLRELISTPGGGNDLTNLTARLPRLEQLTSRAFPRTI
                     RTLDRSQDFVDTLRQYTPDLAGAVSKLGQVTNSYDANGHYARVQPMFVPFSANPANGE
                     LTAVPPSQRGAGYQTKRWRRCPGAAMQAPPDGSAPIQVDGCSPASRPGG"
     gene            630..2087
                     /locus_tag="VFY44_00070"
     CDS             630..2087
                     /locus_tag="VFY44_00070"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5908853.1"
                     /translation="MRRLLAIIALGLALPVLVVLGMGADGDSGSGYEVRAIFDNVASA
                     VPGEDVKVAGAKIGVIKSMDVTKDKKAAVVLQIEDARFTPFHADAKCTIRPQSLIGEK
                     FVECNPGTAASPALARIGDGDQHLLPLKQTSSPVDLDLINNINRRPYRERLSILLNEF
                     GTGLAGRGKDLNEVIHRANPALRETDKVLAILAKQNQVLGRLAVNSDRALAPLAREKR
                     QLTGFIESAGDTATATAERREDIARGIERFPGFLRELQPLMADLQGFAEQATPVATNL
                     NRSGRDVSRVFQALGPFSQGAQPSLETLGDALRTGRPAVLRTRPLVQDVGRFAKQARP
                     LSSDLDKLTASIDKTDGIERLMETIYFTTLVINGFDEDGYYQRANLLNNLCSTYQVST
                     QDGCSARFEGGASASAASAGTPPKPANAPAPGKEPTASTGATSSAPVLEGLLGSGVGK
                     KGREAAEGVRRRAGAPGAKAKPDEAALDYLLGGER"
     gene            2084..3511
                     /locus_tag="VFY44_00075"
     CDS             2084..3511
                     /locus_tag="VFY44_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5908854.1"
                     /translation="MKRGSGASNLTASPVLVGAVTLLVTLVAVYLSYNANSGLPFVPT
                     YDLKADVPNAANLVRGNDVRIGGARVGTVSKITPVPSASGKPAARLDLKLDRSIEPLP
                     VDTTFIVRPRSALGLKYVELSPGRAKAGFTAGATIPARSARPEPVEIDDVFNMFDDRT
                     RRGAQRSLNGFGTGLAGRGQDLNEAIQAFKPLLGNLEPVARNLSSSQTRLDRLFPALE
                     ATASEVAPVAETQAALFGNLNTTFTALAGVARPFLQDFITESPPTLETGTREFPRQRP
                     FLRNSAAFFGELRPGVATLPSSAPVLADALEAGARTLPKTPPLNRRLAGVFDSLEEFA
                     DDPLVPRGIRRLRDTAASLRPTVAFITPAQTQCNYVSLLLRNLASLNSEGDSKGTYER
                     FIIVASPQGPNNIGSPSSAPANGPEPDNYLHANPYPNTASPGQVKECEAGQEPWVQGR
                     RVLGNVPGNQGLITEGKPKKSGAGQ"
     gene            3523..>4904
                     /locus_tag="VFY44_00080"
     CDS             3523..>4904
                     /locus_tag="VFY44_00080"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5908855.1"
                     /translation="MTPLRAGAIGIVVLTVFVFFAFAKDIPFTKGYEMKAVFQNSSAL
                     QLNSPVRIAGVDVGKVSKVEPLNGDSTLTEVTMKIEKKGLPIHRDARLKIRPRIFLEG
                     NFFVDVRPGTPGSPDLPDGGTIGQAQTSAPVQLDQVLGTLKSSARADLQKLLVGYGES
                     INGKPTAAEDATQDPATQGQTAGESLNDSLEDAPDALRQSAIVNEAFLGTSPGDLARL
                     IKGNQRVAAALVRSEGNLKDLITNFNTTTGALASRQGDLRATLRELPQVLEAANPAFD
                     SLNASFPATRAFAREIIPGVRETPATIEASLPWIAQTRALVRPSELGGSIALLQPAVR
                     DLAKFTDGTVKFLPQADAFNRCALNNLLPTGDAVIRDGGLSTGVSNQDEFFQSLVGLS
                     GESQNFDGNGNYVRFTTGGGSNTQATGPINGGPNLFANFIKQPLGTRPARPLRTPPIL
                     RTTPCYTQKRP"
BASE COUNT          763 a         1894 c         1650 g          597 t
ORIGIN      
        1 aaccgcgcgc tctcggaggc gctcgcgctg ctgccgacca cgctgcggcg cggcaactcc
       61 acgttcgtga acctgcgctc gacgctggac gacctggacg tgctggtcga cgagtccaag
      121 ccggccacca agcgcctgcc caccctgttc aaccggctga agctgctgac cgccgagtcg
      181 cggcccaccg tccgcagcct gcgcgagctc atcagcaccc ccgggggcgg caacgacctg
      241 accaacctga cagcgcggct gccgcggctc gagcagctga cctcgcgcgc gttcccgcgc
      301 accatccgca cgctcgaccg ctcgcaggac ttcgtggaca cgctgcgcca gtacacgccc
      361 gatctcgcgg gcgcggtcag caagctcggc caggtgacca actcctacga cgccaacggg
      421 cactacgcgc gcgtgcagcc catgttcgtg ccgttctcgg ccaaccccgc gaacggggag
      481 ctgaccgcgg tcccgccctc gcagcgcggg gcgggctacc agaccaagcg ctggcgccgc
      541 tgtcccggcg cggccatgca ggcgcccccc gacggctcgg cgccgatcca ggtggacggc
      601 tgcagccccg cctcgaggcc cggcggctga tgcggcgact gctggcgatc atcgcgctgg
      661 gactggccct gcccgtgctg gtcgtgctgg gcatgggcgc cgacggcgac agcggctcgg
      721 gctacgaggt ccgggcgatc ttcgacaacg tcgcctccgc ggtgcccggc gaggacgtca
      781 aggtcgccgg ggccaagatc ggcgtcatca agtcgatgga cgtgaccaag gacaagaagg
      841 ccgcggtcgt gctccagatc gaggacgccc ggttcacgcc cttccacgcc gacgcaaagt
      901 gcaccatccg gccgcagtcg ctgatcggcg agaagttcgt ggagtgcaac ccgggcacgg
      961 ccgcgagccc ggcgctggcg cgcatcggcg acggcgacca gcacctgctg ccgctcaagc
     1021 agaccagctc gccggtggac ctggacctga tcaacaacat caaccgccgc ccctaccgcg
     1081 agcggctgtc gatcctgctc aacgagttcg gcaccggcct ggccggccgc ggcaaggacc
     1141 tgaacgaggt catccaccgc gccaaccccg cgctgcgcga gaccgacaag gtgctggcca
     1201 tcctggccaa gcagaaccag gtgctcggcc gcctggccgt gaactcggac cgcgccctgg
     1261 ccccgctggc gcgcgagaag cgccagctca ccggcttcat cgagtcggcg ggcgacacgg
     1321 ccaccgccac cgccgagcgg cgtgaggaca tcgcccgcgg gatcgagcgc ttcccgggct
     1381 tcctgcgcga gctccagccg ctgatggccg acctccaggg cttcgccgag caggccacgc
     1441 cggtggccac gaacctgaac cggtcgggcc gcgacgtcag ccgcgtgttc caggcgctcg
     1501 ggccgttctc gcagggggcg cagccctcgc tggagacgct gggcgacgcg ctccgcaccg
     1561 gccggccggc cgtgctgcgc acccggccgc tggtacagga cgtcggccgc ttcgccaagc
     1621 aggcgcgccc gctgtccagc gacctggaca agctcacggc cagcatcgac aagaccgacg
     1681 gcatcgagcg gctgatggag accatctact tcacgacgct ggtgatcaac ggcttcgacg
     1741 aggacggcta ctaccagcgc gcgaacctgc tgaacaacct gtgctcgacc taccaggtga
     1801 gcacccagga cggctgcagc gcccgcttcg agggcggtgc ctcggcctcc gccgcctccg
     1861 ccgggacgcc gcccaagccg gccaacgccc cggccccggg caaggagccg accgcgagca
     1921 cgggcgccac ctccagcgcc ccggtgctgg agggcctgct gggcagcggc gtcggcaaga
     1981 agggccgcga ggccgccgag ggcgtccgcc gccgcgccgg tgcgcccggc gccaaggcca
     2041 agcccgacga ggccgcgctg gactacctgc tgggaggcga gcgatgaagc gcggctccgg
     2101 cgcctcgaac ctgaccgcca gccccgtgct ggtgggcgcg gtgaccctgc tggtgaccct
     2161 ggtcgccgtc tacctgtcct acaacgccaa ctccggcctg ccgttcgtgc cgacctacga
     2221 cctgaaggcc gacgtgccca acgcggccaa cctggtgcgc ggcaacgacg tgcgcatcgg
     2281 cggcgcgcgc gtgggcacgg tgagcaagat cacgccggtc cccagcgcca gcggcaagcc
     2341 cgccgcccgg ctggacctca agctcgaccg ctcgatcgag ccgctgcccg tggacacgac
     2401 cttcatcgtg cgcccgcgct cggccctggg cctgaagtac gtggagctct cgcccggccg
     2461 ggccaaggcc gggttcaccg ccggggccac gatccccgcc cgctccgcgc ggcccgagcc
     2521 ggtggagatc gacgacgtgt tcaacatgtt cgacgaccgc acccggcggg gcgcgcagcg
     2581 ctcgctgaac ggcttcggca ccggcctggc cgggcgcggc caggacctga acgaggcgat
     2641 ccaggccttc aagccgctac tggggaacct ggagccggtg gcgcgcaacc tgtccagctc
     2701 gcagacccgg ctggaccggc tcttccccgc gctggaggcc accgcctccg aggtggcgcc
     2761 ggtggccgag acccaggccg cgctgttcgg caacctgaac accaccttca ccgccctggc
     2821 cggcgtcgcg cggccgttcc tgcaggactt catcaccgag agcccgccca cgctggagac
     2881 cggcacgcgc gagttcccgc gccagcggcc gttcctgcgc aacagcgccg ccttcttcgg
     2941 cgagctgcgc ccgggcgtgg ccacgctgcc gtcctccgcg ccggtgctgg ccgacgcgct
     3001 ggaggccggc gcgcggacgc tgcccaagac cccgccgctg aaccggcgcc tggccggcgt
     3061 gttcgacagc ctcgaggagt tcgccgacga cccgctcgtg cccaggggca tccgccgcct
     3121 gcgcgacacc gccgcctcgc tgcggccgac cgtcgccttc atcacgcccg cgcagacgca
     3181 gtgcaactac gtcagcctcc tgctgcgcaa cctggccagc ctgaactccg agggcgactc
     3241 caagggcacc tacgagcgct tcatcatcgt cgcctcgccg cagggcccga acaacatcgg
     3301 cagcccctcc agcgcgccgg cgaacggccc ggagcccgac aactacctgc acgccaaccc
     3361 gtaccccaac accgcctcgc cgggccaggt caaggagtgc gaggccggcc aggagccctg
     3421 ggtccagggc cggcgcgtgc tgggcaacgt gcccggcaac cagggcctga tcaccgaggg
     3481 caagcccaag aagtcggggg cgggccagtg agggaccgcg gcatgacccc gctgcgcgca
     3541 ggcgccatcg gcatcgtggt cctgacggtg ttcgtgttct tcgcgttcgc caaggacatc
     3601 ccgttcacca agggctacga gatgaaggcg gtgttccaga actcgtcggc gctgcagctg
     3661 aactccccgg tgcggatcgc gggcgtcgac gtgggcaagg tctcgaaggt cgagccgctg
     3721 aacggcgact ccacgctgac cgaggtcacg atgaagatcg agaagaaggg cctgccgatc
     3781 caccgcgacg cgcggctcaa gatccgcccg cggatcttcc tggagggcaa cttcttcgtg
     3841 gacgtccggc ccggcacgcc cgggtcgccg gacctgcccg acggcggcac catcggccag
     3901 gcgcagacgt cggcgccggt gcagctggac caggtgctgg gcacgctcaa gtccagcgcg
     3961 cgcgccgacc tgcagaagct gctcgtgggc tacggcgagt ccatcaacgg caagccgacg
     4021 gcagccgagg acgccaccca ggatcccgca acgcagggcc agaccgccgg cgagtcgctg
     4081 aacgactcgc tggaggacgc gcccgacgcg ctgcgccagt cggccatcgt gaacgaggcc
     4141 ttcctgggca ccagcccggg tgacctggcc cggctgatca agggcaacca gcgcgtcgcc
     4201 gccgcgctgg tgcgcagcga gggcaacctc aaggacctga tcaccaactt caacaccacc
     4261 accggcgcgc tggccagccg ccagggcgac ctgcgcgcga cgctgcgcga gctgccgcag
     4321 gtgctggagg ccgccaaccc ggcgttcgac tccctgaacg cgtccttccc ggccacgcgc
     4381 gccttcgccc gcgagatcat cccgggcgtc cgcgagaccc cggccacgat cgaggcgtcg
     4441 ctgccctgga tcgcgcagac gcgggcgctg gtgcggccca gcgagctggg cggctcgatc
     4501 gccctcctgc agccggccgt gcgggacctg gccaagttca ccgacggcac ggtcaagttc
     4561 ctgccgcagg ccgacgcctt caaccgctgc gcgctgaaca acctgctgcc caccggcgac
     4621 gcggtcatcc gcgacggcgg gctgtcgacc ggggtcagca accaggacga gttcttccag
     4681 agcctggtcg ggctgtccgg tgagtcgcag aacttcgacg gcaacggcaa ctacgtgcgc
     4741 ttcaccaccg gcggcggctc gaacacccag gccacggggc cgatcaacgg cgggcccaac
     4801 ctgttcgcca acttcatcaa gcagccgctg ggcacccgcc cggcgcggcc cttgcgcacc
     4861 ccgccgatcc tgcggaccac cccctgttac acgcagaagc ggcc
//
LOCUS       DASUPW010000005         3523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_554732, whole genome shotgun sequence.
ACCESSION   DASUPW010000005 DASUPW010000000
VERSION     DASUPW010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554732"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VFY44_00085"
     CDS             complement(<1..588)
                     /locus_tag="VFY44_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX5908856.1"
                     /translation="MRQKSPETYEEKLEQLNELREAAAHTNPEAEEKQHDRGKMTARE
                     RVLKLLDEDSFQELDTFVRHRTSDFDMQKNRPWGDAVVTGHGTINGRPVCVFSQDFTV
                     FGGSLGEVMAEKMVKIMDLAAKIGCPVIGINDSGGARIQEGVVSLGAYGDVFVRNVQC
                     SGVIPQISVIMGPCAGGAVYSPAMTDFIFMVKETSH"
     gene            662..1171
                     /locus_tag="VFY44_00090"
     CDS             662..1171
                     /locus_tag="VFY44_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908857.1"
                     /translation="MNLIPRRGAALLTAAILCSATATAAQAATPRGSYGGKVRGTGEG
                     QKLKFKVSGGKVRSTRARFTLTCNGPYATVTYKLRGSARVKRNRTFRLVRRATVKTRG
                     GSVFKVRLTVAGKFNRAGTRAVGTVRGRGTLTEPGTTLVGGLPEPGETSSCTTPPGKQ
                     KFRVKRSRR"
     gene            complement(1175..1672)
                     /locus_tag="VFY44_00095"
     CDS             complement(1175..1672)
                     /locus_tag="VFY44_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908858.1"
                     /translation="MNRTLVKSPPELWSEVSEVESLAQHLGEFGEIAISRLDPEHTVA
                     WEGEHARGTVELEESGWGTKVTLSADLVVAEPEPAPEPEPEPEPEPEPEPVAVRAPWY
                     RRWFSRPAPAREPEPELEVEPEPEPEPDDDPVTVVDPVRATAVLEDTLDHLGAAHHRP
                     FSREG"
     gene            complement(1743..2801)
                     /locus_tag="VFY44_00100"
     CDS             complement(1743..2801)
                     /locus_tag="VFY44_00100"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5908859.1"
                     /translation="MPELTTLERVRGSLARSLKNLPEGVKARLAGEPVVLDGQTLDRD
                     VQVMLAVQEKMGEPPMESLPVDRARTYYEHEFCAIAPPAEPVYHVEELTLPGPAGEVP
                     ARLYSPPPADEGLPMLVHFHGGGHVVGSPDTCDHASRLAVNGAGAAVLAVDYRMGPEN
                     PMPASAEDCEAAFRFAVENAGRFGVDANRIAVGGDSAGGNLGAVVSQAMKAAGGPMPA
                     FQWLIYPSTALGTRTRSYELFEDGFLLTGDLMEWFQSNFLAGGDRSDLRASPLHAEDL
                     SGLPPAYVATGGFDPLRDEGEQYAARLREAGVPVALQRFETLPHGFANLTGISPAAHD
                     AMQQAVGALRMGLAARKA"
     gene            2785..>3523
                     /locus_tag="VFY44_00105"
     CDS             2785..>3523
                     /locus_tag="VFY44_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908860.1"
                     /translation="MVSSGTGADHIPLKSIGRTADHLPIDSSAWSRPQPDEPRDDRGL
                     RRAFREEVREATRVTGISAGLIGVIAFPLWGVFDYLVAPDQATEFVVLRLAFTVPLLG
                     IWLAMISPLGRRRPELLMLGMMVAIEVAIALMVVRVETQHAAYAMGMTLPLFASAFLL
                     VWSWRYTLALAVIGVLALVAATLTAPATPSTAALTTVGFYVGTASLLAFFAQLHRERL
                     AWREFSSRAELEREQARNLELVDELARL"
BASE COUNT          461 a         1303 c         1237 g          522 t
ORIGIN      
        1 atgcgaggtc tccttgacca tgaagatgaa atcggtcatc gccggggagt agaccgcgcc
       61 tccggcgcac gggcccatga tcacgctgat ctggggtatc acccctgagc actgcacgtt
      121 gcggacgaag acgtcgccgt aggcgcccag cgagacgacg ccctcctgga tgcgggcgcc
      181 gcccgagtcg ttgatgccga tcaccgggca gccgatcttg gccgccaggt ccatgatctt
      241 caccatcttc tcggccatga cctcgcccag cgagccgccg aacacggtga agtcctggct
      301 gaacacgcac accggccggc cgttgatcgt gccgtgcccg gtcaccaccg cgtcgcccca
      361 gggccggttc ttctgcatgt cgaagtcgga cgtgcggtgg cggacgaagg tgtccagctc
      421 ctggaagctg tcctcgtcca gcagcttgag cacccgctcg cgggcggtca tcttgccgcg
      481 gtcgtgctgc ttctcctccg cctccggatt ggtgtgggcg gccgcctcgc gcagctcgtt
      541 cagctgctcg agcttctcct cataggtctc ggggctcttc tggcgcatgg ggcgcggcat
      601 cctacctggg agccgacggt tggggctggt gcgccgggtc cgcgcgcctc atgattggcg
      661 catgaacctc atcccgcggc gcggcgccgc cctgctcacg gccgcaatcc tctgctcggc
      721 cacggcgacg gcggcgcagg cggccacgcc gcgcgggtcc tacgggggca aggtcagggg
      781 caccggcgag ggccagaagc tcaagttcaa ggtctcgggc ggcaaggtcc gcagcacccg
      841 ggcgcggttc accctcacct gcaacgggcc ctacgccacc gtcacctaca agctgcgtgg
      901 gtcggcccgg gtcaagcgca accgcacgtt ccgcctggtg cggcgggcca ccgtcaagac
      961 caggggcggc agcgtgttca aggtccggct cacggtcgcc gggaagttca accgcgccgg
     1021 cacgcgggcg gtgggcacgg tcaggggccg cggcacgctc accgagcccg gcacgacgct
     1081 ggtcggcggg ctgcccgagc cgggcgagac gtcgtcgtgc acgacgccgc ccggcaagca
     1141 gaagttccgc gtgaagcgct cgcggcgcta gcgctcagcc ttcgcgggag aaggggcggt
     1201 ggtgggccgc gcccaggtgg tccagcgtgt cctcgagcac cgccgtggca cgcaccgggt
     1261 ccaccaccgt gaccgggtcg tcgtccggct cgggctcggg ctccggttcg acctcgagtt
     1321 cgggctcggg ctcgcgcgcc ggagcggggc gtgagaacca gcgccggtac cacggcgccc
     1381 tgaccgcgac gggctctggc tccggctcgg gctccggctc cggttcgggc tcgggggccg
     1441 gctccggctc ggcgaccacc aggtcggccg agagcgtcac cttcgtgccc catcccgact
     1501 cctcgagctc cacggtgccg cgggcgtgtt cgccctccca ggcgaccgtg tgctcgggat
     1561 ccagccggct gatggcgatc tcgccgaact cgcccagatg ctgggcaagc gattcgacct
     1621 cgctcacctc gctccacagc tcgggaggcg acttgacgag ggttcggttc acggagagat
     1681 cggccatcga agccccggtt cgcccgaccc cgggcgactc cttccgcgtg aaacggcgtg
     1741 ggtcaggctt tgcgcgcagc caagcccatc cggagcgcgc cgacggcctg ttgcatggca
     1801 tcgtgcgccg caggcgagat gcccgtgagg ttcgcgaagc cgtgcggcag ggtctcgaag
     1861 cgctgcagcg cgacgggcac accggcctcg cgcaggcggg cggcgtactg ctcgccctcg
     1921 tcgcgcagcg ggtcgaaccc gcccgtggcc acgtaggcgg gaggcaggcc cgacaggtcc
     1981 tcggcgtgca gcggcgaggc ccggaggtcg gagcggtcgc cgccggccag gaagttggac
     2041 tggaaccact ccatcaggtc cccggtcagc aggaacccgt cctcgaacag ctcgtacgag
     2101 cgggtcctgg tgcccagcgc ggtggagggg tagatcagcc actggaaggc cggcatcggc
     2161 ccgccggcgg ccttcatcgc ctggctgacc accgcgccca ggttgccgcc ggcgctgtca
     2221 ccgccgaccg cgatccggtt cgcgtccacg ccgaaccggc ccgcgttctc gaccgcgaac
     2281 cggaacgcgg cctcgcagtc ctcggccgag gccggcatcg ggttctcggg ccccatccgg
     2341 tagtccacgg ccagcaccgc cgcgcccgcg ccgttcaccg ccagccgcga ggcgtgatcg
     2401 cacgtgtcgg ggctgcccac cacgtggccg ccgccgtgga agtgcaccag catcggaagg
     2461 ccctcgtcgg cggggggagg cgagtacagg cgcgcgggca cctcgccggc gggccccggg
     2521 agcgtcagct cctccacgtg gtacaccggc tcggccggcg gcgcgatggc gcagaactcg
     2581 tgctcgtagt aggtccgcgc ccggtccacg ggcagcgact ccatcggcgg ctcgcccatc
     2641 ttctcctgca cggccagcat gacctggacg tcgcggtcca gcgtctggcc gtccagcacc
     2701 accggctctc cggccagccg ggccttgacg ccctcgggca ggttcttgag cgaacgcgcc
     2761 aggctcccgc ggacgcgctc gagtgtggtc agctctggca ccggcgcaga ccatatcccg
     2821 ctcaagtcca tcggccgaac ggccgaccat ctacccatcg actcgagcgc ctggtcccgc
     2881 ccccagcccg acgagccgcg cgacgaccgc ggcctgcgtc gcgcattccg cgaggaggtc
     2941 cgcgaggcca cgcgcgtcac cggcatctcc gccggcctga tcggcgtcat cgccttcccg
     3001 ctgtggggcg tgttcgacta cctggtcgcg cccgaccagg ccacggagtt cgtggtcctg
     3061 cgcctggcct tcaccgtgcc cctcctgggc atctggctgg ccatgatcag cccgctgggg
     3121 cggcgccggc cggagctgct gatgctgggg atgatggtgg cgatcgaggt cgcgatcgcg
     3181 ctgatggtgg tccgcgtgga gacccagcac gcggcctacg ccatgggcat gaccctgccc
     3241 ctgttcgcca gcgcgttcct gctggtgtgg tcgtggcgct acacgctcgc cctggccgtg
     3301 atcggcgtgc tggccctggt ggccgccacc ctgacggcgc ccgcgacacc gtccacggcg
     3361 gcgctgacca ccgtcgggtt ctacgtgggc accgcctcgc tcctggcctt cttcgcccag
     3421 ctgcaccgcg agcgcctggc ctggcgcgag ttctccagcc gcgccgagct ggagcgcgag
     3481 caggcgcgca acctcgagct cgtggacgag ctggcccggc tga
//
LOCUS       DASUPW010000006         3419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1940037, whole genome shotgun sequence.
ACCESSION   DASUPW010000006 DASUPW010000000
VERSION     DASUPW010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1940037"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..1498
                     /locus_tag="VFY44_00110"
     CDS             80..1498
                     /locus_tag="VFY44_00110"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX5908861.1"
                     /translation="MTLRDLMGDGAPEVEITGLAYSSKSVSDGTLFFCVPGFKVDGHD
                     FAPDAVERGAAALVCQRPLGLGVPEVVVDDVRATMGPVASAFFGDPTSKMQVAGITGT
                     NGKTTTAFLMHGVLETSGLHAGLLGTVKQIVGGLEEEAVRTTGEAIDLQETFVRMRNL
                     GDQACVMEVSSHALELGRADGITFAVKVFTNLTQDHLDFHPDMEAYYLAKRRLFDLPG
                     PAVVNLDDDYGVRLAGELGDATTFALEGGPEADYTARDIRFDVSGAVFTVDTPDGAVG
                     MTTRLPGLFNVSNALAAFTAARVMGIEAEVAAKALADAARVPGRFEPVEEGQDFGVLV
                     DYAHTPDSLENVLRAARELTEGTLHVVFGAGGDRDKAKRPLMGKAAADHADRLVVTSD
                     NPRSEDPDAIVDQVMEGAGPGAEREVDRRKAIAKAVAGAEDGDVVVIAGKGHEQGQEF
                     EDGRKEPFDDVQVAREALRERS"
     gene            1495..2739
                     /gene="murF"
                     /locus_tag="VFY44_00115"
     CDS             1495..2739
                     /gene="murF"
                     /locus_tag="VFY44_00115"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX5908862.1"
                     /translation="MKRFERAVIDTREVRPGDLFVGLRGERTDGGRFAADALAAGAAG
                     VLVAPEHAAGLEGGTVITADDPLAALQEMARDWRRELGAKVIGVTGSTGKTSTKDILT
                     ALLAHHRRTHANRQNLNTEIGLPLTILEAPPGTEVLVLEMAMRGQGQIAELVAIAEPD
                     VGVIVNVGPVHLELLGTVERVAAAKAELIRDLAPGAACVVPASEPLLDAHLRDDLETT
                     TFGPGGNVNLLAFDAGRAEIDVGDSTLELDLPFSEPYNLLNTLAGVAAALAVGVRPSG
                     RVEPEFSSMRGEIVELGGGVTVVNDCYNANPMSMRAALKHLAETPADRRVAVLGTMAE
                     LGPDSAAFHHEIGREAAELDVDVLITVGAEAVPYADEFDGESYSTATPEEAGALLSEI
                     ALPGDRVLIKGSRSVGLERVLA"
     gene            2763..3359
                     /locus_tag="VFY44_00120"
     CDS             2763..3359
                     /locus_tag="VFY44_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908863.1"
                     /translation="MASLLICMFLGPKFIAWLRAQEFGQHIREEGPEGHHGKAGTPTM
                     GGIVIFAAVAVPFLILTDYRAVSMAVFATAIATAGLGLADDLAKVRKKRSLGVSGRTK
                     LAAQAVIAIGLWLVATEYVGLPPTLRLRFVDASVDLGLFYPVLILLVLMGATNGVNLT
                     DGLDGLAAGCCAIVLLSFTAMCFITAGPGQEDLALRSA"
BASE COUNT          473 a         1189 c         1286 g          471 t
ORIGIN      
        1 gactggccgg cgatggtgga ctggtgcctg gacggcgcca cgaggttccg tcacacgcgg
       61 gcatagactt ggccccacca tgaccctgcg agacctgatg ggtgatgggg ctcccgaggt
      121 cgagatcacg gggctggcgt actcgagcaa gagcgtctcc gacgggacgc tcttcttctg
      181 tgtgcccggc ttcaaggtcg acggccacga cttcgccccc gacgcagtgg agcgcggcgc
      241 agccgcgctg gtgtgccagc gcccgctcgg cctgggcgtg cccgaggtgg tggtggacga
      301 cgtccgggcc acgatgggcc cggtggcctc cgccttcttc ggcgatccca cgtcgaagat
      361 gcaggtggcg ggcatcaccg gcaccaacgg caagaccacc acggccttcc tgatgcacgg
      421 ggtgctggag accagcggtc tgcacgcggg gctgctgggc acggtcaagc agatcgtcgg
      481 cggcctggag gaggaggcgg tgcgcaccac gggcgaggcc atcgacctcc aggagacgtt
      541 cgtgcgcatg cgcaacctgg gcgaccaggc ctgcgtgatg gaggtgtcct cacacgcgct
      601 ggaactgggc cgggccgacg gcatcacctt cgccgtcaag gtgttcacca acctgaccca
      661 ggaccacctg gacttccatc ccgacatgga ggcctactac ctggccaagc ggcggctgtt
      721 cgacctgccc gggccggcgg tggtgaacct ggacgacgac tacggcgtgc ggctggccgg
      781 cgagctgggc gacgccacca ccttcgcgct cgagggcggg cccgaggccg actacacggc
      841 gcgcgacatc cgattcgacg tgagcggggc ggtgttcacc gtggacaccc ccgacggcgc
      901 ggtgggcatg accacgcggc tgccggggct gttcaacgtg tccaacgcgc tggccgcctt
      961 caccgccgcg cgggtcatgg ggatcgaggc cgaggtggcg gccaaggcgc tggccgacgc
     1021 ggcccgcgtg cccggccgct tcgagcccgt cgaggagggc caggacttcg gcgtcctggt
     1081 cgactacgcg cacaccccgg actcgctgga gaacgtcctg cgcgccgccc gcgagctgac
     1141 cgagggcacc ctgcacgttg tcttcggcgc cggcggcgac cgcgacaagg ccaagcggcc
     1201 gctgatgggc aaggccgccg ccgaccacgc cgaccggctg gtggtcacct ccgacaaccc
     1261 ccgctcggag gacccggacg ccatcgtcga ccaggtcatg gagggcgccg ggcccggtgc
     1321 cgagcgcgag gtcgaccgcc gcaaggcgat cgcgaaggcc gtggcgggcg ccgaggacgg
     1381 cgacgtggtg gtcatcgccg ggaaggggca cgagcagggc caggagttcg aggacggccg
     1441 caaggagccg ttcgacgacg tgcaggtggc ccgcgaggcg ctgagggagc gctcgtgaag
     1501 cggttcgagc gcgccgtgat cgacacgcgc gaggtgcgcc cgggcgacct gttcgtgggt
     1561 ctccgcggcg agcgcaccga cggcggccgg ttcgcggccg acgccctggc cgccggcgcc
     1621 gccggggtgc tggtggcgcc cgagcacgcg gccgggctgg aagggggcac ggtgatcacc
     1681 gccgacgacc cgctggccgc cctccaggag atggcgcgcg actggcgccg cgagctcggc
     1741 gccaaggtga tcggcgtcac cggctccacc ggcaagacgt cgacgaagga catcctgacg
     1801 gcgctgctgg cccaccaccg gcgcacgcac gccaaccgcc agaacctgaa caccgagatc
     1861 gggctgccgc tgaccatcct ggaggcgccg ccgggcacgg aggtgctggt gctggagatg
     1921 gccatgcgcg gccagggcca gatcgccgag ctggtggcca tcgccgagcc cgacgtgggc
     1981 gtgatcgtca acgtcggccc cgtgcatctg gagctgctgg gcacggtcga gcgcgtggcc
     2041 gcggccaagg ccgagctgat ccgcgacctc gcgcccggcg cggcctgtgt ggtcccggcg
     2101 tccgagccgc tcctggacgc ccacctgcgc gacgacctgg agaccaccac cttcggcccc
     2161 ggcggcaacg tcaacctgct ggccttcgac gccgggcggg ccgagatcga cgtgggcgac
     2221 tccacgctgg agctcgacct gcccttctcc gagccctaca acctgctgaa cacgctggcg
     2281 ggcgtggccg cggccctggc cgtgggcgtg cggccctcgg gccgcgtgga gcccgagttc
     2341 tcgtccatgc gaggcgagat cgtggagctc gggggcgggg tgaccgtggt caacgactgc
     2401 tacaacgcca acccgatgtc catgcgggcc gcgctcaagc acctggccga gacgcccgcc
     2461 gaccggcgcg tggcggtgct gggcaccatg gccgagctgg gccccgactc cgccgccttc
     2521 caccacgaga tcggccgcga ggcggccgag ctggacgtgg acgtgctgat cacggtgggc
     2581 gccgaggcgg tgccctacgc ggacgagttc gacggcgagt cctactccac cgccacgccc
     2641 gaggaggccg gcgcgctgct gtcggagatc gcgctcccgg gcgaccgggt gctcatcaag
     2701 ggctcgcgct ccgtgggcct cgagcgggtg ctggcgtagt gggcgagatc ctgatcgggg
     2761 ggatggcctc cctgctgatc tgcatgttcc tgggccccaa gttcatcgcg tggctgcggg
     2821 cgcaggagtt cggccagcac atccgcgagg aggggcccga gggccaccac ggcaaggccg
     2881 gcacgcccac catgggcggc atcgtgatct tcgccgccgt ggcggtgccg ttcctgatcc
     2941 tgaccgacta ccgggcggtg agcatggccg tgttcgccac cgcgatcgcc acggccgggc
     3001 tggggctggc cgacgacctg gcgaaggtgc gcaagaagcg ctcgctgggc gtgtcgggca
     3061 ggaccaagct ggccgcacag gccgtgatcg cgatcggcct gtggctggtg gccaccgagt
     3121 acgtgggcct gccgccgacg ctgcggctgc ggttcgtgga cgcctcggtg gacctgggcc
     3181 tgttctaccc ggtgctgatc ctgctggtgc tcatgggcgc caccaacggc gtgaacctga
     3241 ccgacgggct cgacgggctg gcggccggct gctgcgcgat cgtgctcctg tccttcaccg
     3301 ccatgtgctt catcaccgcc gggccggggc aggaggacct ggcgctgcgg tcggcgtgac
     3361 tggtcggagc ctgcgtgggc ttcctgtggt tcaactcggt cccggcctcg gtgttcatg
//
LOCUS       DASUPW010000007         2916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1802051, whole genome shotgun sequence.
ACCESSION   DASUPW010000007 DASUPW010000000
VERSION     DASUPW010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802051"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VFY44_00125"
     CDS             complement(<1..619)
                     /locus_tag="VFY44_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5908864.1"
                     /translation="MEAGTAAATGTGSKTMADMVGLAATQYGDSPALRFKTGEEWSDV
                     SFAALGEAVGEIGRGLIDLGVEHAGKVAIVSHTRAEWTYAHFGILAAGAISVSVYQTN
                     SAEECHYVLEHSESEVVFLEDAEQLEKIRAVRDRLPNLRQIVLFEPVEGADDAITLDE
                     LRERGRGRDAAELEARTAAVTKDDVCIFIYTSGTTGPPKCCILSHG"
     gene            complement(663..851)
                     /locus_tag="VFY44_00130"
     CDS             complement(663..851)
                     /locus_tag="VFY44_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908865.1"
                     /translation="MANHLTPTELSRETGLDREEVIAKCVELGVPIFQGKIDKKLFAA
                     TVEHDEGGSEHSPGWARA"
     gene            complement(905..1663)
                     /locus_tag="VFY44_00135"
     CDS             complement(905..1663)
                     /locus_tag="VFY44_00135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEX5908866.1"
                     /translation="MAHRDQIVSYLDDLLDAGAYPDMTPNGLQVPGADTVEVVVTGVS
                     PGLELFQAAAERGAQLVLTHHGLLGDFLPDHVTPVLKGRLKALFDADLSLASYHLPLD
                     AHAQVGNNALICAELGLERAEPFGSYKGTTLGFVGRSQAGIPFAELRERCARAFGTEP
                     FAWEYGPEVVHSVGVVSGAAASSLGEAVAGGLDAFLTGEPAEHVMLEAREAGIHFIAG
                     SHYGTETFGVKRLGELLADRFGIRHEFVDMPNPV"
     gene            1688..1942
                     /locus_tag="VFY44_00140"
     CDS             1688..1942
                     /locus_tag="VFY44_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908867.1"
                     /translation="MGTESKGGSEADFLESLTDSGLYSMGAFFCDEHPDLVDDVIAQS
                     EAIEQAGIRTYAAEHDLAPEACFETLLTGLAVRYYNAVAA"
     gene            1961..2034
                     /locus_tag="VFY44_00145"
     tRNA            1961..2034
                     /locus_tag="VFY44_00145"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1995..1997,aa:Pro,seq:cgg)
     gene            complement(2016..>2916)
                     /locus_tag="VFY44_00150"
     CDS             complement(2016..>2916)
                     /locus_tag="VFY44_00150"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX5908868.1"
                     /translation="TGATGFVGGALARRLMESGHDVRGLVRDRAKASDLEQEGVELHE
                     GDVTDAASLEGAGAGADVAYYLVHGMGRGGKGDFEQTERESATNFAHMARDAGVARVV
                     YLGGLGDNPGSKHLRSRNETGKILGQEGPPLTYFRASMVVGAKSESYLTLRHLVQKLP
                     IMIAPSWLKTKTQPIAVEDVLDYLVAAPSVEEAAREVQIGCPDVVSYGEMLDRMALAL
                     DRRPRPKIPAPILSPWLSSQWIGLVTPVDAGVAKPLVEGLSTETTVTDESGAALFDVE
                     PMGIDEALRHAVAEERGYRAAQI"
BASE COUNT          404 a         1056 c         1007 g          449 t
ORIGIN      
        1 tgccgtggct gaggatgcag cacttgggcg ggccggtggt gcccgacgtg tagatgaaga
       61 tgcacacgtc gtccttggtc accgcggccg tgcgcgcctc cagctcggcg gcgtcgcggc
      121 cgcggccgcg ctcgcgcagc tcgtcgaggg tgatggcgtc gtccgcgccc tcgaccggct
      181 cgaacaggac gatctgacgc aggttgggca gccggtcgcg gacggcgcgg atcttctcca
      241 gctgctcggc gtcctccagg aacacgacct cggactcgga gtgctcgagc acgtagtggc
      301 attcctcggc cgagttggtc tggtagaccg agaccgagat ggcgccggcg gccaggatgc
      361 cgaagtgggc gtaggtccac tccgcgcgcg tgtgggacac gatggccacc ttgcccgcgt
      421 gctccacgcc caggtcgatc aggccgcgcc cgatctcacc gacggcctcg cccagagcgg
      481 cgaagctgac gtcggaccac tcctcacccg tcttgaaccg tagcgccggg ctgtccccgt
      541 actgcgtcgc ggccaacccg accatgtcgg ccatcgtctt cgaaccggtg cccgtggcgg
      601 ccgccgttcc tgcttccatc gtggtccctc ctccgctcct cagcttggag ggggatacta
      661 gatcaggcgc gggcccagcc gggcgagtgt tcgctgccgc cctcgtcgtg ctcgaccgtc
      721 gcggcgaaca gcttcttgtc gatcttgccc tggaagatcg gcacccccag ctcgacgcac
      781 ttcgcgatga cctcctcgcg gtccagtccc gtctcgcgac tgagctcggt gggcgtcagg
      841 tggttcgcca tctaagtgcg ctcctctgct gggttgccgt tacgtcccag cgtacccgct
      901 ggcgctacac cggattgggc atatccacga attcgtgacg gatcccgaaa cggtcggcca
      961 ggagctcgcc caggcgcttc acgccgaagg tctcggtgcc gtagtgcgac ccggctatga
     1021 agtggatccc ggcctcgcgc gcctcgagca tcacgtgctc ggcgggctcg ccggtcagga
     1081 aggcgtccag gcccccggcc acggcctcgc ccagcgacga cgccgccgcg ccggagacca
     1141 caccgacgct gtggacgacc tcgggcccgt actcccaggc gaacggctcg gtgccgaacg
     1201 cgcgggcgca gcgctcgcgc agctcggcga aggggatgcc ggcctgggag cgcccgacga
     1261 agcccagcgt cgtccccttg tagctgccga acggctcggc ccgctccagc cccagctcgg
     1321 cgcagatgag cgcgttgttg cccacctggg cgtgcgcgtc cagcggcagg tggtacgagg
     1381 ccagcgagag gtcggcgtcg aacagcgcct tcagccggcc cttgagcacg ggcgtgacgt
     1441 ggtcgggcag gaagtccccg agcaacccgt ggtgggtcag caccagctgc gcgccgcgct
     1501 cggcggcggc ctggaacagc tccagcccgg gcgatacccc ggtgaccacg acctccacgg
     1561 tgtctgcgcc gggcacctgg agcccgttcg gcgtcatgtc cgggtaggcg ccggcgtcca
     1621 gcaggtcgtc caggtaggac acgatctgat cgcggtgggc cacgccggga aggtaccctg
     1681 cccgcccatg ggcacggagt caaagggggg gagcgaggcc gacttcctcg agtccctgac
     1741 cgactcgggc ctgtactcga tgggggcgtt cttctgcgac gagcaccccg acctggtcga
     1801 cgacgtgatc gcccagagcg aggcgatcga gcaggccggc atccgcacct acgccgccga
     1861 gcacgacctg gcgccggagg cctgcttcga gacgctcctg accggcctgg cagtccggta
     1921 ctacaacgcg gtcgccgcct gactatcctt ccgcgagcca cgggctgtgg cgcagcctgg
     1981 tagcgcgctc cgttcgggtc ggagaggtcc ccagttcaaa tctgggcagc ccgatatccc
     2041 cgctcttcgg ctacggcatg ccgtagggcc tcgtcgatgc ccatcggctc gacgtcgaac
     2101 agggcggcac cggactcgtc ggtgaccgtg gtctcggtgg acaggccctc gaccagcggc
     2161 ttggccaccc cggcgtccac gggcgtcacc agcccgatcc actgcgacga cagccagggg
     2221 gacaggatcg gcgccgggat cttgggacgg ggccggcggt ccagcgccag ggccatgcgg
     2281 tcgagcatct cgccgtagga gaccacgtcg gggcagccga tctgcacctc gcgcgccgcc
     2341 tcctcgacgg agggggccgc gaccaggtag tccaggacgt cctcgaccgc gatcggctgg
     2401 gtcttggtct tcagccacga cggggcgatc atgatcggca gcttctgcac caggtggcgc
     2461 agggtcaggt aggactcgct cttggcgccg accaccatcg acgcgcggaa gtaggtcagg
     2521 ggcgggccct cctggcccag gatcttgccc gtctcgttgc ggctgcgcag gtgcttggag
     2581 cccgggttgt ccccgaggcc gcccaggtag accacccgcg ccacgccggc gtccctggcc
     2641 atgtgcgcga agttggtggc cgactcgcgc tcggtctgct cgaagtcgcc cttgccgccg
     2701 cggcccatgc cgtggaccag gtagtaggcc acgtccgcgc cggcgcccgc gccctccagg
     2761 gaggcggcgt cggtcacgtc accctcgtgc agctccacgc cctcctgctc caggtcggag
     2821 gccttggccc ggtcgcgcac caggccgcgg acgtcgtgcc ctgactccat cagcctgcgg
     2881 gccagcgccc cgccgacgaa ccccgtcgcg cctgtg
//
LOCUS       DASUPW010000008         3771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1802196, whole genome shotgun sequence.
ACCESSION   DASUPW010000008 DASUPW010000000
VERSION     DASUPW010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802196"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VFY44_00155"
     CDS             <1..838
                     /locus_tag="VFY44_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908869.1"
                     /translation="GLKGRDRDLREVFRRLGPLHRDLAALQGEVVKRRRNLARLVHNY
                     GSTVDRLGREDRDLSTLVSGANRVFGRLSREDGNISEAVSRLPGTLSQTEATLRRVRV
                     LGQEAGPAFEALRPAVRQIDQTNRQLRPLAETAEPVLRKQVRPFVRAARPYVDQVRPA
                     ATNLAKASPDLRESFFELNRFFNMAAYNPGGRERLTGDAQKDVARDEGMLFWLGWVAH
                     NSNLVFSTGDAQGPYRRIILNATCTTYRQILEQLGAAGPAAATIIGLQNLLADTDLCP
                     PS"
     gene            865..2298
                     /locus_tag="VFY44_00160"
     CDS             865..2298
                     /locus_tag="VFY44_00160"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5908870.1"
                     /translation="MLAMSGFALSCVCLLLFLWLQFGGSVPLRAESYRFKAQFDEAAL
                     LVKQADVRIAGLNVGKVVELEPKDGRSVATIEIEPRYGPIPRDTRVILRQKALLGETY
                     IELSPGNRAGGTLDDGDTLAVKATQEAVQIDEIVRAFDRPTRRAFQGWIRELAGAIEK
                     GRGEDLNNAIGNLPPFVATGDDVLAVLDDQRPALQALIRNSGRTLEAVNEREGQLRQL
                     VVNGDRFFGALASRNEALADTVFVLPTFLDETRLTIRRLRRFSVNARPLVRDLQPVAT
                     DLKPTLRDVGRLAPDLKALFRDLSPLIDESEDTLPAAARFLRGAEPVFEGLHAYLPEL
                     NPILSFANFQQSQLSDFIMNGAGALCATLPPINSSEGPRHYLRQFGAINARGLGLASK
                     RPEWERGNAYPGPNYLKRSRPLGIVESFDCKPAGGTKRNATEGSPPCFVQPKSLFDGR
                     QFPLLRKGGSPLRNPPPDNLGSAPASP"
     gene            2303..3601
                     /locus_tag="VFY44_00165"
     CDS             2303..3601
                     /locus_tag="VFY44_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5908871.1"
                     /translation="MTSIAVVGAGPAGLTALVALARAGLDVTCFEKGDRPGGLWAYGA
                     PLSGAYRSLHLNTSRARTELAGFPMPADWPDFPSHEHIGRYFADYVEHAGVGDRIRYG
                     AAVTRAERGAGGGWAVAAEDGFEGRFDHLVVASGHNWEPAWPDPPYPGSFDGVQVHAH
                     DYREPDLFEGRRVLVVGMGNSAMDIAVEASTVAERVLLSTRSGTRIVPKYVFGKPADQ
                     ATSPAMARLPWQLRQPLTHAMLRVAVGKPESYGLPPASGGFLRSHPTISDSVLSRLTH
                     GEIEVRPGVKSLQGGAVEFTDQGRDDVDVIVWCTGYRVTVPYVDDDVLGAPPAELPLF
                     KRMFHHTHDDLFFIGLVQTTGSAIPVVERQSALLADHLTGRYKPPAKARRKVDSERRR
                     RAAARRYGEHKRPHLRVEFDGFMRELATEHKRGRRRAGRR"
BASE COUNT          516 a         1409 c         1361 g          485 t
ORIGIN      
        1 ggggctgaag ggacgcgacc gcgacctgcg cgaggtcttc cgccggctcg ggccgctgca
       61 ccgcgacctg gcggccctcc agggcgaggt ggtcaagcgc cgccgcaacc tcgcccggct
      121 ggtgcacaac tacggctcga ccgtcgaccg gctggggcgc gaggaccgcg acctgtcgac
      181 gctggtcagc ggcgccaacc gcgtgttcgg ccgcctcagc cgcgaggacg gcaacatctc
      241 cgaggcggtc agccgcctgc ccgggacgct gtcgcagacc gaggccacgc tccggcgggt
      301 gcgggtgctg ggccaggagg ccgggccggc cttcgaggcg ctgcgccccg cggtccgcca
      361 gatcgaccag accaaccgcc agctgcgccc gctggccgag accgcggagc cggtgctgcg
      421 caagcaggtg cgaccgttcg tgcgcgccgc gcggccgtac gtggaccagg tccggccggc
      481 ggccacgaac ctggccaagg cctcgccgga cctgcgcgag tcgttcttcg agctcaaccg
      541 cttcttcaac atggccgcct acaacccggg cgggcgcgag cgcctgacgg gcgacgcgca
      601 gaaggacgtg gcccgcgacg agggcatgct gttctggctg ggctgggtgg cacacaactc
      661 caacctggtc ttctccaccg gcgacgccca ggggccctac cggcggatca tcctgaacgc
      721 cacctgcacg acctaccggc agatcctgga gcagctggga gcggccggcc ccgccgccgc
      781 gaccatcatc gggctccaga acctgctggc cgacaccgac ctctgcccgc cgtcgtgatc
      841 aaggaagccc caagcctcgg acggatgctg gccatgtcag gcttcgcgct gtcgtgcgta
      901 tgcctgctgc tcttcctctg gctccagttc ggcggctcgg tgccgctgcg ggccgagtcg
      961 taccgcttca aggcccagtt cgacgaggct gccctgctgg tcaagcaggc cgacgtccgg
     1021 atcgccggcc tgaacgtggg caaggtcgtg gagctggagc cgaaggacgg gcgcagcgtg
     1081 gccacgatcg agatcgagcc gcggtacggg cccatcccga gggacacccg cgtgatcctg
     1141 cgccagaagg cgctgctggg cgagacctac atcgagctgt cgcccggcaa ccgcgccggg
     1201 gggacgctgg acgacggcga cacgctggcg gtcaaggcca cccaggaggc ggtgcagatc
     1261 gacgagatcg tgcgcgcgtt cgaccgcccg acgcggcgcg ccttccaggg ctggatccgc
     1321 gagctggcgg gggcgatcga gaaggggcgc ggggaggacc tgaacaacgc catcggcaat
     1381 ctgccgccgt tcgtggccac cggcgacgac gtgctggccg tcctggacga ccagcggccg
     1441 gcgctccaag cgctgatccg caactcgggc cggacgctgg aggcggtcaa cgagcgcgag
     1501 ggccagctgc gccagctggt ggtcaacggc gaccgcttct tcggcgccct ggcctcgcgc
     1561 aacgaggcgc tggccgacac cgtgttcgtg ctgcccacct tcctggacga gacgcggctg
     1621 accatccggc gcctgcgccg cttctcggtg aacgcgcggc cgctggtgcg cgacctccag
     1681 cccgtggcca ccgacctcaa gccgaccctg cgcgacgtcg gccgcctggc gccggacctg
     1741 aaggccctgt tccgcgacct gagcccgctg atcgacgagt ccgaggacac gctgccggcg
     1801 gcggcgcggt tcctgcgcgg agccgagccg gtgttcgagg gcctgcacgc ctacctgccc
     1861 gagctgaacc cgatcctgtc cttcgccaac ttccagcaga gccagctgtc ggacttcatc
     1921 atgaacgggg ccggtgccct ttgcgcgacg ctgccgccga tcaactccag cgaggggccg
     1981 cgccactacc tgcgccagtt cggcgccatc aacgcacgcg gcctgggcct ggcctccaag
     2041 cgccccgagt gggagcgcgg caacgcctac cccggcccca actacctcaa gcgctcgcgc
     2101 ccgctgggca tcgtcgagtc gttcgactgc aagcccgccg gcggcaccaa gcgcaacgcc
     2161 acggagggct cgccgccctg cttcgtgcag cccaagtcgc tgttcgacgg gcgccagttc
     2221 ccgttgctgc gcaagggtgg ctcgccgctg cgcaacccgc cgccggacaa cctcggctcg
     2281 gccccggcca gcccgtgacg ccatgaccag catcgccgtg gtgggggccg gccccgccgg
     2341 actgaccgcg ctggtggcgc tggcccgcgc cggcctggac gtgacctgct tcgagaaggg
     2401 cgaccggccc ggcgggttgt gggcctacgg cgccccgctg tccggcgcct accggtcgct
     2461 gcacctgaac accagccgcg cccgcaccga gctggcgggc ttcccgatgc ccgcggactg
     2521 gcccgacttc ccctcgcacg agcacatcgg gcgctacttc gccgactacg tcgagcacgc
     2581 cggggtgggg gacaggatcc gctacggcgc cgcggtcacc cgggccgagc gtggcgccgg
     2641 cgggggctgg gcggtcgcgg ccgaggacgg gttcgagggc cgcttcgacc acctggtggt
     2701 ggccagcggc cacaactggg agccggcgtg gcccgacccg ccgtacccgg gctccttcga
     2761 cggcgtccag gtgcacgccc acgactaccg cgagccggac ctgttcgagg gccggcgcgt
     2821 gctggtggtg ggcatgggca acagcgccat ggacatcgcg gtcgaggcct cgaccgtcgc
     2881 cgagcgggtg ctgctgtcca cccgctcggg cacccggatc gtgcccaagt acgtgttcgg
     2941 caagcccgcc gaccaggcca cctcgccggc catggcccgg ctgccctggc agctgcgcca
     3001 gccgctgacc cacgcgatgc tgcgggtggc cgtcggcaag cccgagagct acggcctgcc
     3061 gccggcgtcg ggcgggttcc tgcgctcgca ccccaccatc agcgattcgg tgctctcgcg
     3121 cctgacccac ggcgagatcg aggtccggcc cggggtcaag tcgcttcagg gcggggccgt
     3181 cgagttcacc gaccaggggc gcgacgacgt ggacgtgatc gtctggtgca ccggctaccg
     3241 ggtcacggtg ccctacgtgg acgacgacgt cctgggcgcg cccccggcgg agctgccgct
     3301 gttcaagcgc atgttccacc acacgcacga cgacctcttc ttcatcggcc tggtgcagac
     3361 cacgggctcc gccatcccgg tggtcgagcg ccagagcgcg ctgctggccg accacctgac
     3421 cgggcgctac aagccgccgg cgaaggcgcg ccgcaaggtc gactccgagc gccgccggcg
     3481 ggcggccgcg cgccgctacg gcgagcacaa gcgcccccac ctgcgggtcg agttcgacgg
     3541 cttcatgcgc gagctggcca ccgagcacaa gcgcggccgg cgccgggccg ggcgccggtg
     3601 acccgcgtcc tggtcacggg cgcggggggc gccttcggga gcgcggcgac ggcggcgctg
     3661 cgcgcacgcg gcgcgacggt catggggctg gacctggtgg ccgatcccga ggcggggatc
     3721 ctggagtgcg acatcaccga cccggaggcg gccgcgcgcg gggtggggga g
//
LOCUS       DASUPW010000009         6325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1248394, whole genome shotgun sequence.
ACCESSION   DASUPW010000009 DASUPW010000000
VERSION     DASUPW010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248394"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1526)
                     /locus_tag="VFY44_00170"
     CDS             complement(87..1526)
                     /locus_tag="VFY44_00170"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5908872.1"
                     /translation="MEAGQGKAMQPAARRPSGVARIAALAAVLAAATLTGLLLFGGGD
                     GYTVTARFINAGQLVKGNLVDVGGTRAGVVKDFEITANGQADVVLQIDEKYAPLRHGT
                     RAVIRQGGQASPANRYVQLMLPPEPQAGNRIRDGGRIGIEMTTTNVDLDQFFNIFDRP
                     TRKALQDFYKGGQRQYAGRAEAANRGLRYLSPQLAASSRLFAELRHDPPVLERFLVDS
                     SRFVTALSSRRDDLSALIANLNSTTRGLGSEEAALSEAITRFPPFLRQANSTYVNLRA
                     ALDDLDPLVNASKPVARKLRPYLGELRPLARDAVPTVRNLRRTVRRAGGANDLRELQQ
                     TYPKLEEVALVERRRNGAERRGAFPELSEALRGSAPIIAHGRPYTTDLFGWFDDFSHT
                     GASDALGSFSRVQTYINAFTVQNGLPTNLIPLADRTNVFRSMARLRQVKRCPGGAEVP
                     AADGTNVLSAEQQRELDCRESDRATGDLP"
     gene            1609..1764
                     /locus_tag="VFY44_00175"
     CDS             1609..1764
                     /locus_tag="VFY44_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908873.1"
                     /translation="MERRPDRPEPEETDDEEYPAGTEVPTDPPGVDPEEGEDEGPGFG
                     DDPPQAD"
     gene            complement(1847..3784)
                     /locus_tag="VFY44_00180"
     CDS             complement(1847..3784)
                     /locus_tag="VFY44_00180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5908874.1"
                     /translation="MSAFQLRRPAVTVPRVAAIARPELIALVALALALNLWGLDRNGF
                     ANEYYAAAVRSMGSSWHAFLYGSFDASGVMTVDKPPLASWVQVASVKVFGYGSWGLLI
                     PQALMGAASVALVYDLVRRPFGRAAGTVAGLVLALTPIAVAVSRHNNPDALLVLCSVA
                     AVWCCARALEDGRTRWLVLSGVAVGLGFETKMAVALMVVPGIVAAYLWIAPRGLRAAV
                     GQVAAFTGAAVAVGAAWPLLLALTPSAGRPWVSGTADNSIWSLIFGYNGLGRLVGQSG
                     GPGGVAGGPGGGGPPGGGGAGSMFGGPTGFTRLVESSLGGQAGWLIGAALVGGAAVVL
                     ASRLRRSDVRSGWVVLVGGAFLTCAVAFSTAKGIFHPYYVSLLAPFTAALVGALVPHV
                     TGGDMTARVLACLGVTGAVATELMVLHNLPGELTWLRPVLFVAGAGLVALLAVGLPAR
                     ARRIVLVAALGLVLMAPATWAVQTLGHATNGTFPSGGPQTMGMGGGPGPGGGAMGGPP
                     GGAAPAGGGGGGPFGGNTQTLTQAVAYARAHGGGAVATGSQQGAAGPIIESGADVVAI
                     GGFSGRESQVDAKWLAEAVGDGRVRWVLADQGGMGGPQDSRVGDRAVMSTVAGTCTPV
                     SSVTGLYDCSGSASALAKAGS"
     gene            3875..5068
                     /locus_tag="VFY44_00185"
     CDS             3875..5068
                     /locus_tag="VFY44_00185"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyltransferase family 2/GtrA
                     family protein"
                     /protein_id="HEX5908875.1"
                     /translation="MPDQALLPGPAPSLRPGPQVEVVVPVYNEEAVLARSIGRLHRLL
                     SGDFPFSWRIVIADNASTDATPRIAARLARQLPGVRALRLEEKGRGRALRTAWSASDA
                     DVVCYMDVDLSTDLRALLPLIAPLLSGHSDVSIGTRLAHGSHVVRGPKREFISRTYNH
                     LLRATLRTRFSDAQCGFKAVRAGALPKLLPQVRDQGWFFDTELLVTAQRAGLRIHEVP
                     VDWADDPDSRVDIAATALEDLKGIARLASGTAAVRFLAVGACSTLAYVLLFLALRGPL
                     GPAWANALALALTAVGNTAANRLFSFRVRGRERLVRHHVLGLVVFLITLGLTSGALGV
                     LHALDPGPSRAMELAVLVASSTVATISRFVALRSWVFADSGPVAPEQVVGVDPLLGHD
                     GRAQG"
     gene            complement(4994..6172)
                     /locus_tag="VFY44_00190"
     CDS             complement(4994..6172)
                     /locus_tag="VFY44_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HEX5908876.1"
                     /translation="MPERLSVPRDRDDDYTAEAQQARLDFLREQTGAEMPHVAGKAVE
                     PAETAGNVEHFIGSAQVPLGLAGPLLVDGEHAKGEFYVPMATAEGTLVASYNRGMRLL
                     HEAGGVKVTVTEDHMQRAPAFIFPDARGARDFGIWLTENFDAIKAAAEETTSTGRLQE
                     VEQYSASRILFTRFNYTTGDAAGQNLTGKATAAACKWIVENYAPGIERFFLESNFATD
                     KKTSQVNVLNTRGKRVVAEAVIPNELMKSVMRSDSALLFQARQTSSLGGFMAGVNNNG
                     SHSANGITAMFIATGQDAANVAESSAALVYAELRENGDYYYSITIPSLIVATYGGGTG
                     LPTQRECLEAMDCYGAGKVRKFAEIVGATVLCGELSLGSAIVAEEWVDAHDLLGRNRP
                     "
BASE COUNT          853 a         2359 c         2300 g          813 t
ORIGIN      
        1 gcgcgcgccg tcaccgccgc cccccaccag caccaccgtg gcggcgacca gggcgaggac
       61 cgcgacggcg gtgaccagcc ggcgcatcag ggcaggtccc cggtggcccg gtcggactcg
      121 cggcagtcga gctcgcgctg ctgctcggcg ctgagcacgt tggtgccgtc ggcggccggc
      181 acctcggcgc cgcccgggca gcgcttgacc tggcgcagcc gcgccatcga gcggaagacg
      241 ttggtgcggt cggccagcgg gatcaggttc gtgggcaggc cgttctgcac ggtgaacgcg
      301 ttgatgtagg tctgcacgcg cgagaagctg cccagcgcgt cgctggcgcc ggtgtgcgag
      361 aagtcgtcga accagccgaa caggtcggtg gtgtacggcc gcccgtgggc gatgatcggc
      421 gcgctcccgc gcagcgcctc cgacagctcg gggaaggcgc cgcggcgctc ggccccgttg
      481 cggcggcgct ccaccagcgc cacctcctcc agcttggggt aggtctgctg cagctcgcgc
      541 aggtcgttgg cgccgcccgc gcggcgcacg gtgcgccgca ggttgcgcac ggtgggcacc
      601 gcgtcgcgcg ccagcgggcg cagctcgccc aggtacgggc gcagcttgcg cgccaccggc
      661 ttcgaggcgt tcaccagcgg gtccaggtcg tccagggccg cgcgcaggtt cacgtaggtg
      721 gagttggcct ggcgcaagaa cggcgggaag cgggtgatgg cctccgacag cgccgcctcc
      781 tcgctgccca ggccgcgcgt ggtggagttg aggttggcga tcagcgccga caggtcgtca
      841 cggcgcgagg acagcgcggt cacgaagcgc gagctgtcga ccaggaagcg ctccagcacc
      901 ggcgggtcgt ggcggagctc ggcgaacagg cggctggagg cggcgagctg ggggctgagg
      961 tagcgaagtc cgcggttggc ggcctcggcg cggccggcgt actggcgctg accacctttg
     1021 tagaaatcct ggagcgcctt gcgagtgggc cggtcgaaga tgttgaagaa ctggtccagg
     1081 tccacgttgg tcgtggtcat ctcgatcccg atccgccctc cgtcgcggat ccggttgccc
     1141 gcctggggct cgggcggcag catcagctgc acgtagcggt tggccggcga ggcctgcccc
     1201 ccctggcgga tcaccgcgcg cgtgccgtgg cgcagcggtg cgtacttctc gtcgatctgc
     1261 aggaccacgt cggcctgccc gttggcggtg atctcgaagt ccttgaccac cccggcgcgc
     1321 gtgcccccca cgtccaccag gttgcccttg accagctgac cggcgttgat gaagcgggcc
     1381 gtgaccgtgt agccgtcgcc gccgccgaag agcagcagcc cggtcagcgt cgcggcggcc
     1441 aggaccgccg ccagggcagc gatccgcgcg acgcccgatg ggcgccgcgc cgccggctgc
     1501 atcgccttcc cctgcccagc ttccatgcaa gtctcccggc ctgcatcgcc ctgcctgaaa
     1561 tcaccctaac ccggccgcgg ggcgcatgcg ccacttagga tcccccgcat ggaacgccga
     1621 cccgacaggc ccgagcccga agagaccgac gacgaggagt accccgcggg caccgaggtc
     1681 cccaccgatc ccccgggcgt cgatcccgag gaaggcgagg acgaggggcc ggggttcggc
     1741 gacgatccgc cgcaggcgga ctaggcgctt cggcaggccg gggaggaccg gccggatctc
     1801 ggtccgcgac cacggtccgg gcgttcggga ccaggtgggc ggcggactag ctcccggcct
     1861 tcgccagggc ggacgccgat ccgctgcagt cgtacaggcc ggtgaccgac gagacgggcg
     1921 tgcacgtgcc ggccaccgtg ctcatgaccg cccggtcgcc cacgcggctg tcctgcgggc
     1981 cgcccatgcc gccctggtcg gccagcaccc agcgcacgcg gccgtcgccg accgcctcgg
     2041 ccagccactt ggcgtcgacc tggctctcgc ggcccgagaa gccgccgatg gccaccacgt
     2101 cggcgccgga ttcgatgatc ggccccgccg caccctgctg gctgccggtg gccaccgcgc
     2161 cgccgccgtg ggcgcgggcg taggccacgg cctgggtcag ggtctgggtg ttgccgccga
     2221 acgggccgcc gccaccgccg cccgcgggcg cggcgccgcc gggaggcccg cccatggccc
     2281 cgccgccggg gcccgggccg ccgcccatgc ccatcgtctg ggggccgccg gaggggaacg
     2341 tgccgttggt ggcgtggccc agcgtctgca ccgcccacgt ggcgggcgcc atcagcacca
     2401 gcccgagggc cgcgaccagg acgatgcggc gcgcccgggc tggcaggccg acggccagca
     2461 gggccaccag gccggcgccg gccacgaaca ggacagggcg tagccaggtc agctcgcccg
     2521 gcaggttgtg caggaccatc agctcggtgg ccacggcgcc ggtcacgccc aggcaggcca
     2581 ggacgcgcgc ggtcatgtcg ccgccggtca cgtgcggcac cagcgcgccc accagggcgg
     2641 cggtgaacgg cgccagcagc gacacgtagt acgggtggaa gatgcccttg gcggtgctga
     2701 aggccaccgc gcaggtcagg aacgcgccgc ccaccaggac gacccatccg ctgcgcacgt
     2761 cgctgcggcg cagccggctg gccagcacca ccgcggcgcc gcccaccagc gccgcgccga
     2821 tcagccagcc cgcctggccg cccaggctgg actcgaccag ccgcgtgaac ccggtcgggc
     2881 cgccgaacat cgagcccgcg ccgccgccgc caggaggccc gccgccgccg gggccgccgg
     2941 ccacgccacc gggcccgccg gactggccca ccaggcgccc gaggccgttg tagccgaaga
     3001 tcagcgacca gatgctgttg tccgccgtgc ccgacaccca cggccggccg gccgacgggg
     3061 tcagcgccag cagcagcggc cacgcggcgc cgacggccac ggcggcgccg gtgaacgcgg
     3121 ccacctggcc gacggcggcg cgcaggccgc gcggggcgat ccacaggtag gcggccacga
     3181 tgccgggcac caccatcagc gccaccgcca tcttggtctc gaagcccagg ccgacggcga
     3241 cgccgctgag caccagccag cgcgtgcgcc cgtcctccag cgcccgggcg cagcaccaca
     3301 cggcggccac cgagcacagc accagcagcg cgtcggggtt gttgtggcgc gagacggcaa
     3361 cggcgatcgg ggtcagcgcc agcaccagcc cggccaccgt gccggcggcg cgcccgaacg
     3421 ggcgccggac caggtcgtag accagggcca ccgaggcggc gcccatcagc gcctgcggga
     3481 tcagcaggcc ccaggagccg tagccgaaca ccttgaccga ggccacctgg acccaggagg
     3541 ccagcggcgg cttgtccacg gtcatgaccc ccgacgcgtc gaacgagccg tacaggaacg
     3601 cgtgccagct cgagcccatc gaccgcaccg ccgcggcgta gtactcgttg gcgaagccgt
     3661 tgcggtccag cccccacagg ttcagcgcca gggccagcgc gaccagcgcg atcagctcgg
     3721 gtcgggcgat ggcggccacg cgggggacgg tcacggcggg gcggcgaagt tggaatgcgc
     3781 tcatggcggt caccgtggcc cggctccgta agagcaacgg aagggcggcc accgtcttac
     3841 gtcgctccga cagaggcgca gcacagtgcg ctccatgccc gaccaagccc tcctccccgg
     3901 cccggccccc tccctccgcc cgggcccgca ggtggaggtc gtcgtaccgg tgtacaacga
     3961 ggaggcggtg ctggcgcgca gcatcgggcg cctgcaccgc ctcctgtcgg gcgacttccc
     4021 gttcagctgg cggatcgtga tcgccgacaa cgccagcacc gacgccacgc cccggatcgc
     4081 cgcccgcctg gcccgccagc tgcccggggt ccgggccctg cgcctggagg agaaggggcg
     4141 cgggcgcgcc ctgcggacgg cgtggtcggc cagcgacgcg gacgtggtct gttacatgga
     4201 cgtcgacctg tcgacggacc tgcgcgcgct gcttcccctg atcgcgccgc tgctgtcggg
     4261 ccacagcgac gtgtcgatcg gcacccgcct ggcgcacggc tcccacgtgg tccgcggccc
     4321 caagcgcgag ttcatctcgc gcacgtacaa ccacctgctg cgcgcgacgc tgcgcacccg
     4381 gttcagcgac gcccagtgcg ggttcaaggc cgtgcgcgcc ggcgcgctgc ccaagctgct
     4441 gccgcaggtc cgcgaccagg gctggttctt cgacaccgag ctgctggtga ccgcccagcg
     4501 tgccggcctg cgcatccacg aggtcccggt ggactgggcc gacgacccgg actcgcgcgt
     4561 tgacatcgcg gccaccgcgc tcgaggacct gaagggcatc gcccgcctgg cctcgggcac
     4621 ggcggccgtg cgcttcctcg cggtcggcgc ctgctcgacg ctggcctacg tgctgctgtt
     4681 cctggcgctg cgcgggccac tgggccccgc gtgggccaac gcgctggcgc tggcgctgac
     4741 cgcggtgggc aacaccgcgg ccaaccgcct gttcagcttc cgcgtgcgcg ggcgcgagcg
     4801 actggtgcgc caccacgtcc tgggcctggt ggtgttcctg atcaccctcg ggctgaccag
     4861 cggcgccctg ggcgtgctgc acgcgctgga ccccggcccc agccgggcga tggagctggc
     4921 cgtgctcgtg gcctcgagca ccgtggccac catcagccgg ttcgtggcgc tgcgcagctg
     4981 ggtgttcgcc gactcagggc cggttgcgcc cgagcaggtc gtgggcgtcg acccactcct
     5041 cggccacgat ggccgagccc agggatagct cgccgcacag caccgtcgcg cccacgatct
     5101 cggcgaactt gcggaccttg ccggcgccgt agcagtccat cgcctccagg cactcgcgct
     5161 gggtgggcag cccggtgccg ccgccgtagg tggccacgat cagcgacggg atggtgatcg
     5221 agtagtagta gtcgccgttc tcgcggagct cggcgtacac cagcgccgcc gaggactcgg
     5281 ccacgttggc ggcgtcctgg ccggtggcga tgaacatggc cgtgatgccg ttggccgagt
     5341 gcgagccgtt gttgttcacg ccggccatga agccgcccag cgacgaggtc tggcgcgcct
     5401 ggaacagcag cgcgctgtcc gagcgcatga cgctcttcat cagctcgttg gggatcaccg
     5461 cctcggccac cacccgcttg ccgcgcgtgt tcagcacgtt cacctgcgag gtcttcttgt
     5521 cggtggcgaa gttggactcc aggaagaagc gctcgatgcc cggtgcgtag ttctccacga
     5581 tccacttgca cgcggcggcg gtggccttgc cggtcaggtt ctggcccgcg gcgtcaccgg
     5641 tcgtgtagtt gaagcgcgtg aacaggatcc gcgaagccga gtactgctcg acctcctgca
     5701 acctgccggt cgaggtcgtc tcctccgccg cggccttgat cgcgtcgaag ttctcggtca
     5761 gccagatccc gaagtcgcgg gcgccgcggg cgtcggggaa gatgaacgcg ggtgcgcgct
     5821 gcatgtggtc ctcggtcacc gtgaccttga cgccgccggc ctcgtgcagc aggcgcatgc
     5881 cgcggttgta gctggccacc agggtgccct cggcggtcgc catcggcacg tagaactctc
     5941 ccttggcgtg ctcgccgtcc accagcagcg ggccggccag ccccagcgga acctgggccg
     6001 agccgatgaa gtgctcgacg ttgcccgcgg tctcggccgg ctccaccgcc ttgcccgcca
     6061 cgtgcggcat ctcggcgccc gtctgctcgc gcaggaagtc cagccgggcc tgctgggcct
     6121 cggcggtgta gtcgtcgtcg cggtcgcggg gtacggagag gcgctcgggc atgggcgcga
     6181 gcctagtgcg gcgttaagcg aacgccagcc gcgccgcgat cgccttccgc gaggcgaaca
     6241 ggtacgctgc cacgaagacc agcgacagca cgcacacgat gcacccgccg gtcgaggcgt
     6301 ccaggtggaa gctcacgtag gtgcc
//
LOCUS       DASUPW010000010         2072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_971356, whole genome shotgun sequence.
ACCESSION   DASUPW010000010 DASUPW010000000
VERSION     DASUPW010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971356"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..424)
                     /locus_tag="VFY44_00195"
     CDS             complement(50..424)
                     /locus_tag="VFY44_00195"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX5908877.1"
                     /translation="MEIERIQDWVGADVVDPAGEKVGKLEDLYYVPGEREPVAAAVKY
                     GTLGKSVGLVPLTGASVTRSYLRISHSKDTLKHSPDVQPATDLASSTVSEAADHYGAS
                     LPAGELESGARRQQSADAAAPR"
     gene            complement(434..>2072)
                     /locus_tag="VFY44_00200"
     CDS             complement(434..>2072)
                     /locus_tag="VFY44_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX5908878.1"
                     /translation="RVVVVDPRRTRTAEVADEHHFIRPGHDAHLMAAMAWTLFDEGLV
                     DTGTVGDHLEGLDQLRGLLADFSPEAVAPACGIGAEQIRRLARELAGAERAAVYGRIG
                     TTTQSFGTLASWLVDVLNTLTGNLDREGGAMFTRAAAGQRNSTGRPGSGRGFAIDRWQ
                     TRVRGLPEALGELPVSALAEEIDTPGEGRIRALITQAGNPLVSTPNSGRLAEAVEQLD
                     FMLSIDIYLNETTRHADVVLPAPGPLEKSQYDLALYQLAARNVANFSPAILESEVPPE
                     WQTLARLTGIVSGQGPDVDVDAVDSMVIGAMVAREVGDEHSRIGGRDAGEIMAELEPR
                     RGPERMLDLLLRVGAYGDAFGDDPGGLTLARLEENPHGIDLGPLEPRVPDVLRTASGR
                     IELAPEPIVADLPRLRESLAGAAPEMVLVGRRQLRSNNSWMHNLEPLVKGKDRCTAQL
                     HPDDAERLGLAEGAAAVLRSAAGSVEAPVEITDSVMPGVVSLPHGWGHDQPGMRMAVA
                     AAHHGVNSNFLADESAVDPVSGNAILNGIPVDVSAAG"
BASE COUNT          257 a          779 c          745 g          291 t
ORIGIN      
        1 gacacggcga acgtgatctg ccgcaactgc ggcaacagca tgctccggcc tatctaggcg
       61 ctgccgcgtc ggcgctctgc tggcgtcgcg cgccgctctc cagctcgccc gcgggcaagg
      121 aggcgccgta gtggtcggcc gcctcggaga ccgtgctgga ggccaggtcc gtggccggct
      181 gcacgtcggg ggagtgcttg agcgtgtcct tggagtggct gatccggagg tacgaccggg
      241 tcacgctggc gccggtcagg ggcacgaggc ccacggactt gcccagcgtc ccgtacttga
      301 ccgcggcggc caccggctcg cgctcgccgg gcacgtagta caggtcctcc agcttgccga
      361 ccttctctcc ggccgggtcg acgacgtcgg caccgaccca gtcctggatg cgctcgatct
      421 ccatgcgccg ggcctacccg gcagccgaga cgtcaaccgg gatgccgttc aagatggcgt
      481 tcccggacac ggggtcgacc gcgctctcgt cggccaggaa gttgctgttc acgccgtggt
      541 gggcggcggc caccgccatc cgcatgccgg gctggtcgtg gccccagccg tgcggcaggc
      601 tgaccacgcc gggcatgacc gagtcggtga tctcgaccgg cgcctcgacc gagccggcgg
      661 ccgagcgcag cacggcggcg gcgccctcgg ccaggcccag gcgctccgca tcgtcggggt
      721 gcagctgggc ggtgcagcgg tccttgccct tcaccagcgg ctccaggttg tgcatccagg
      781 agttgttcga gcgcagctgg cgccggccga ccaggaccat ctccggtgcc gcgcccgcca
      841 gcgactcgcg caaccgcggc aggtcggcca cgatcggctc cggtgccagc tcgatccggc
      901 ccgaggccgt gcgcagcacg tcggggacgc gcggctccag cggccccagg tcgatgccgt
      961 gcgggttctc ctccagccgc gccagcgtca gcccgccggg gtcgtcgccg aacgcgtcgc
     1021 cgtaggcgcc cacgcgcagc agcaggtcga gcatccgctc gggcccgcgg cggggctcga
     1081 gctcggccat gatctcgccc gcgtcgcggc cgccgatgcg cgagtgctcg tcgccgacct
     1141 cgcgcgcaac catcgcgccg atgaccatcg agtccacggc gtccacgtcc acgtccggcc
     1201 cctggccgga gacgatgccg gtcagccggg ccagcgtctg ccattccggc ggcacctcgg
     1261 actccaggat cgcgggcgag aagttggcca cgttgcgcgc ggccagctgg tagagcgcca
     1321 ggtcgtactg ggacttctcc agcggcccgg gggcggggag gaccacgtcc gcgtggcggg
     1381 tggtctcgtt caggtagatg tcgatcgaga gcatgaagtc cagctgctcg accgcctcgg
     1441 ccagccggcc cgagttgggc gtggacacca gcgggttgcc ggcctgggtg atcagcgccc
     1501 ggatccggcc ctcgccgggc gtgtcgatct cctccgcgag cgccgacacc ggcagctcac
     1561 ccagcgcctc cggtagaccg cgcacgcgcg tctgccagcg gtcgatggcg aacccgcgcc
     1621 cgcttccggg cctgcccgtg gagttgcgct gccccgccgc cgcgcgcgtg aacatggcgc
     1681 cgccctcgcg atcgaggttg ccggtcagcg tgttcagcac gtccaccagc cagctggcca
     1741 gggtgccgaa cgactgcgtg gtggtcccga tgcgcccgta cacggcggcg cgctcggcgc
     1801 cggccagctc gcgtgcgagc cgccggatct gctccgcgcc gatgccgcac gccggcgcca
     1861 ccgcctcggg gctgaagtcg gccagcagcc cccggagctg gtcgaggccc tccaggtggt
     1921 cgcccaccgt tcccgtgtcc accagcccct cgtcgaacag cgtccaggcc atggccgcca
     1981 tcagatgggc gtcgtggccg gggcggatga agtggtgctc gtcggccacc tcggccgtgc
     2041 gcgtgcgccg cgggtcgacc accaccacgc gg
//
LOCUS       DASUPW010000011         5623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1386936, whole genome shotgun sequence.
ACCESSION   DASUPW010000011 DASUPW010000000
VERSION     DASUPW010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5623
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386936"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VFY44_00205"
     CDS             complement(<1..644)
                     /locus_tag="VFY44_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5908879.1"
                     /translation="MARAVLLLHSSAGLYGADVQLLAIARGLDPERWRPVCVLPERGP
                     LAPLLEQAGAEVVVHPLAVLRRSLATAAGAVRMARATAADQRVLGALARDHGVRVVHD
                     NTSVILGGARVARAAGAAHVVHVRESWTGGRRGERALWPLMRRRLLRADALVGISRSV
                     CDQFPPGAAVLLRDGLPREPVPVDRERARAELGLPPDRFVVAVVGRVHPWKGQDV"
     gene            complement(652..1563)
                     /locus_tag="VFY44_00210"
     CDS             complement(652..1563)
                     /locus_tag="VFY44_00210"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5908880.1"
                     /translation="MRIASLVPSSTEMLFELGLGDSIVAVTHECDHPPAAADLPHLTR
                     SVIPEGLSAAEIDAAVREHVAQGKALYELDEETLTRQDPELIVTQAVCDVCAVSFDDV
                     RAVADRLPSRPEVISLDPTTIGEMLADVPRLAEAAGVPDEGEQLVEVAAERLDDVRDA
                     VASADPISVLALEWLDPVFIGGHWVPQMIEMAGGIDVLGLAGEKSRTAEWAEVEAAAP
                     EVIVSMPCGYGTERAAQETLEHADRLAALGARVAAVDASAYFSRPGPRLVDGVELMAH
                     LLHPGLVPEPPPERFAWLDLDRSGSPA"
     gene            1574..1852
                     /locus_tag="VFY44_00215"
     CDS             1574..1852
                     /locus_tag="VFY44_00215"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX5908881.1"
                     /translation="MARLSEEEIGEQLESAEGWSREGEAITKDFERGDFMGSVGLVND
                     IAPVAEEMGHHPDLEISWDTVTVTISTHSEGGLTAADFDLAQKIDDLG"
     gene            1960..3321
                     /locus_tag="VFY44_00220"
     CDS             1960..3321
                     /locus_tag="VFY44_00220"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX5908882.1"
                     /translation="MTQLLLLIGVAVLVAANGFFVAAEFALVRARGSRIDELAGEGKR
                     GARLAAHQIDHIDEYLSACQLGITMASLGIGFLGEPAIASLIETLLGESVPHGVSLAV
                     SFGMAYFITTALHITVGEQVPKIFSITHAEATVLRTARLLEWFRVAFKPLIWALNTAS
                     NAMLRAIGTNPKAEFDEASSSEDLKLLIAQSQTGGKLDPGEAGMLSGVFHLHEQEARQ
                     VMTPIPAVVTVDVAESAEDALRRCVDSGHTRLVVTEDGNTDRIRGVIHNNSLARRLMN
                     EGPEASIEASVKDALIVPETKPLDDLLADLQRERASIAVVVDEYGRTAGIVSVEDIVE
                     EVVGEIADETDPAVAGVRRLGNGDWWVRGHVPIDDLVDYGLGIPHDSDAYNSVGGFVF
                     GELGRLPKRGDMVRVNGYSLRVESVRENRIEAVRIRDHGSTAPEAATTMEDDEPREAT
                     EGA"
     gene            complement(3318..3743)
                     /locus_tag="VFY44_00225"
     CDS             complement(3318..3743)
                     /locus_tag="VFY44_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908883.1"
                     /translation="MDRPVPHPALFRLGLAALGLVQLTDGLYALLWPRSFYEDFPLGR
                     GWVEAIPAYNPHLLADVGALFIATGVLMLMAAFWLERRLVLAALVTWLLFAVPHVTYH
                     LGNLGPYDTADTIGNVVTLGLTVLIPVALLVVLVRRAPA"
     gene            complement(3781..4563)
                     /locus_tag="VFY44_00230"
     CDS             complement(3781..4563)
                     /locus_tag="VFY44_00230"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5908884.1"
                     /translation="MSEPNPNIRLGIAGSGAIAIGLAKAAGPNVDTVLRARSEESAER
                     ARSRVGDGVRVVTDLGEFGDRTYVVEAISEDHDTKVSLFTEIAGHLADDAVLATTTSS
                     LSVNRLAADSGRPAKFAGLHVFNPVHRMDLVELAFPDDADDATRAASTELCEAMGKTV
                     VEVPDVPGFVVNRLLFPFAFQAVELQQETGLSAEDIDSCMKLGAGHPMGPLALLDFVG
                     LDVSKAIGEQVGAPIPQRVDDLIDQGHLGRKSGSGFYEYSKK"
     gene            complement(4560..5030)
                     /locus_tag="VFY44_00235"
     CDS             complement(4560..5030)
                     /locus_tag="VFY44_00235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtoQ family protein"
                     /protein_id="HEX5908885.1"
                     /translation="MTWTVHLSGEIHTDWRERIAGGARDAGLDVAFTSPVTDHSASDD
                     CGAAILGEPAEQFFRDQLGAGVNAVRTRTQMAKADIVVVRFGEQYKQWNAAFDAGYAA
                     ATGTPLITLHPAEHDHALKEVDRAALAVCREPEQVVAVLRYAVDGELVIGSRPE"
     gene            5092..>5623
                     /locus_tag="VFY44_00240"
     CDS             5092..>5623
                     /locus_tag="VFY44_00240"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5908886.1"
                     /translation="MTGDELLAGRYRTLRLIGTGGMARVFLAEDQRLGRQVAVKRLHA
                     HSPEDIARRFQREARLGAALNHPNLVAVYDVETDGESVLIVMEYVEGTTLADELKAGA
                     LGVDRTVAMISDVAAALDAVHEKGIVHRDVKPANVLLRSKDGMAKLADLGIATAAEST
                     RITVSGTVMGTAAYMAP"
BASE COUNT          821 a         1990 c         2075 g          737 t
ORIGIN      
        1 acgtcctggc ccttccacgg gtgcacccgg cccaccaccg ccaccacgaa ccggtcgggg
       61 ggcaggccga gctcggcgcg ggcccgctcg cggtccaccg gcacgggctc gcgcgggagg
      121 ccgtcgcgca gcaggacggc ggcgccgggc gggaactggt cgcacacgga tcgcgagatg
      181 cccaccaggg cgtcggcgcg aaggagccgg cggcgcatca gcggccacag ggcgcgctcg
      241 ccgcggcggc cgccggtcca ggactcgcgc acgtggacca catgggcggc ccccgccgcg
      301 cgggccaccc tggccccgcc caggatcacc gaggtgttgt cgtgcaccac ccggacgccg
      361 tggtcccggg ccagcgcgcc cagcacgcgc tggtcggcgg cggtcgcccg cgccatccgg
      421 accgcgcccg ccgcggtggc cagcgacctg cggagcaccg ccagcgggtg gaccacgacc
      481 tcggcgcccg cctgctccag cagcggcgcc agcggtccgc gctcgggcag cacgcagacc
      541 ggccgccagc gctcgggatc caggccgcgc gcgatcgcca gcagctgcac gtccgcgccg
      601 tagaggcccg ccgacgagtg cagcagcagg acggcgcggg ccacggcggg cctaggccgg
      661 cgagcccgag cggtccaggt ccagccaggc gaaccgctcg ggtggcggct cgggcaccag
      721 cccggggtgc agcaggtggg ccatcaactc gaccccgtcg accagccgcg ggccggggcg
      781 cgagaagtag gcggacgcgt ccacggccgc cacccgcgcg cccagcgcgg ccagccggtc
      841 ggcgtgctcc agggtctcct gcgccgcccg ctcggtgccg tagccgcagg gcatggagac
      901 gatgacctcg ggcgcggcgg cctcgacctc ggcccactcg gcggtgcgcg acttctcgcc
      961 ggccagcccc agcacgtcga tgccgccggc catctcgatc atctgcggga cccagtgccc
     1021 gccgatgaac accgggtcca gccactccag ggccaggacc gagatcggat cggcggaggc
     1081 caccgcgtcc ctgacgtcgt ccagccgctc ggcggccacc tccaccagct gctcgccctc
     1141 gtcgggcacg ccggcggcct cggccagccg cggcacgtcg gccagcatct cgccgatggt
     1201 ggtggggtcc agcgagatca cctcgggccg ggacggcagc cggtcggcca ccgcgcgcac
     1261 gtcgtcgaag gagacggcgc acacgtcgca caccgcctgg gtcacgatca gctccgggtc
     1321 ctggcgggtc agggtctcct cgtcgagctc gtacagcgcc ttgccctggg ccacgtgctc
     1381 gcgcacggcg gcgtcgatct cggctgccga caggccctcg gggatcacgc tccgcgtcag
     1441 gtggggcagg tccgcggccg ccggcgggtg gtcgcactcg tgcgttactg ccacgatgct
     1501 gtcgccgagt ccgagctcga agagcatctc ggtggaggac ggcaccagtg aggcgattcg
     1561 catggaggga acgatggcac gtctgagcga agaggagatc ggggagcagc tggagtccgc
     1621 ggagggctgg tcgcgcgagg gcgaggccat caccaaggac ttcgagcgcg gcgacttcat
     1681 gggctccgtg ggcctggtga acgacatcgc gccggtggcg gaggagatgg gacaccatcc
     1741 cgacctggag atctcgtggg acacggtcac ggtgaccatc tcgacccatt ccgagggcgg
     1801 gctgaccgcg gccgacttcg accttgcgca gaagatcgac gaccttggtt gacggactgg
     1861 acacgacgtc ctgtccgtct gccggttctc cggctaaggt gtcgaagttc ctcggatgag
     1921 cgacggcgaa cctcctcgaa gtagggcctc ggcccccaca tgacgcagct actcctgctg
     1981 atcggggttg ctgtgctcgt tgcggcgaac ggcttcttcg tggctgcgga gttcgctctg
     2041 gtccgcgccc gcgggtcgcg gatcgacgag cttgccgggg agggcaagcg gggtgcccgc
     2101 ctggcggcgc accagatcga ccacatcgac gaatacctgt ccgcctgtca gctggggatc
     2161 acgatggcct cgctgggcat cggcttcctg ggcgagcccg ccatcgccag cctgatcgag
     2221 accctgctgg gcgagtccgt cccgcatggg gtgtcgctgg ccgtgtcctt cggcatggcc
     2281 tacttcatca ccacggccct gcacatcacg gtcggcgagc aggtgccgaa gatcttctcg
     2341 atcacgcacg cggaggccac cgtcctgcgc acggcgcggc tgctcgagtg gttccgcgtg
     2401 gccttcaagc cgctgatctg ggcgctgaac accgcctcca acgcgatgct cagggccatc
     2461 ggcaccaacc ccaaggccga gttcgacgag gcgtcctcgt ccgaggacct gaagctgctg
     2521 atcgcccaga gccagaccgg cggcaagctc gaccccggtg aggcggggat gctctccggc
     2581 gtcttccacc tgcacgagca ggaggcgcgc caggtcatga cccccatccc cgcggtggtc
     2641 accgtggacg tggccgagag cgcggaggac gcgctgcgcc ggtgcgtgga ctcgggccac
     2701 acccggctgg tggtcaccga ggacggcaac accgaccgca tccgcggcgt gatccacaac
     2761 aactcgctgg cccgcaggct gatgaacgag ggcccggagg cctcgatcga ggccagcgtc
     2821 aaggacgcgc tgatcgtgcc cgagaccaag ccgcttgacg acctgctggc cgacctgcag
     2881 cgcgagcgcg cctcgatcgc cgtcgtggtg gacgagtacg ggcgcaccgc cgggatcgtg
     2941 tcggtcgagg acatcgtcga ggaggtcgtg ggcgagatcg ccgacgagac cgacccggcc
     3001 gtggcggggg tccgccgcct gggcaacggc gactggtggg tgcgcggcca cgtgcccatc
     3061 gacgacctcg tggactacgg gctgggcatc ccgcacgact ccgacgccta caactccgtc
     3121 ggcgggttcg tgttcggcga gctgggccgg ctgcccaagc gcggcgacat ggtgcgggtc
     3181 aacggctatt cgctgcgggt ggagtcggtg cgcgagaacc ggatcgaggc ggtgcgcatc
     3241 cgcgaccacg ggagcacggc gcccgaggcc gcgacgacga tggaggacga cgagccgcgc
     3301 gaggccaccg aaggcgccta ggcgggcgct ctgcgcacca ggaccaccag cagcgcgacc
     3361 gggatcagca ccgtcaggcc cagcgtcacg acgttgccga tggtgtccgc ggtgtcgtag
     3421 ggccccaggt tgcccaggtg gtaggtcacg tggggcacgg cgaacagcag ccaggtgacc
     3481 agcgcggcca gcacgagccg gcgctcgagc cagaaggcgg ccatgagcat gagcaccccg
     3541 gtggcgatga acagggcgcc cacgtcggcc agcagatggg ggttgtaggc ggggatcgcc
     3601 tccacccacc cgcggcccag cgggaagtcc tcgtagaagc tgcgcggcca cagcagcgcg
     3661 tacaggccgt cggtcagctg gaccaggccg agcgcggcca ggccgaggcg gaacagggcc
     3721 gggtggggga cggggcggtc catgtgacct ccgacgagcc cggcccgtcc cccgtgacgc
     3781 ctacttcttg gagtactcgt agaagcccga gccggacttg cggcccaggt ggccctggtc
     3841 gatcaggtcg tcgacgcgct gggggatcgg tgcgcccacc tgctcgccga tggccttcga
     3901 gacgtccagg cccacgaagt ccagcagcgc cagcgggccc atggggtgcc cggcgcccag
     3961 cttcatgcac gagtcgatgt cctcggcgga cagcccggtc tcctgctgca gctccaccgc
     4021 ctggaaggcg aacgggaaca gcagccggtt gaccacgaag cccggcacgt cgggaacctc
     4081 gacgacggtc ttgcccatgg cctcgcacag ctcggtcgag gcggcgcggg tggcgtcgtc
     4141 ggcgtcgtcg gggaaggcca gctcgaccag gtccatccgg tgcacggggt tgaacacgtg
     4201 caggcccgcg aacttggcgg gccgcccgct gtcggcggcc agcctgttca cgctgagcga
     4261 cgaggtcgtc gtggccagca cggcgtcgtc ggccaggtgg ccggcgatct ccgtgaacag
     4321 cgacaccttg gtgtcgtggt cctcgctgat ggcctcgacg acgtaggtgc ggtcgccgaa
     4381 ctcgccgagg tcggtgacca cgcgtacgcc gtcgcccacg cggctgcggg cgcgctcggc
     4441 ggactcctcg ctgcgcgcgc gcagcacggt gtcgacgttc gggccggcgg ccttggccag
     4501 gccgatcgcg attgcgccgc tgccggcgat gcccaggcgg atgttggggt tcggttcgct
     4561 cattcgggcc gagacccaat cacaagctcg ccgtccacgg cgtagcgcag caccgccacg
     4621 acctgctcgg gctcgcggca cacggccagc gcggcgcggt ccacctcctt gagcgcgtga
     4681 tcgtgctcgg cggggtggag cgtgatcagc ggcgtgcccg tcgcggcggc gtagccggcg
     4741 tcgaaggcgg cgttccactg cttgtactgc tcgccgaagc gcaccaccac gatgtcggcc
     4801 ttggccatct gcgtgcgcgt gcgcaccgcg ttcacgccgg cgcccagctg atcgcggaag
     4861 aactgctcgg ccggctcgcc caggatcgcc gcgccgcagt cgtcgctggc ggagtgatcg
     4921 gtgacgggcg aggtgaaggc cacgtccagg cccgcatcgc gggcgccgcc ggcgatccgc
     4981 tcgcgccagt cggtgtggat ctcgccggag aggtgcactg tccaggtcat gcgtccacgc
     5041 taccgcgggg ggtgatgcca tcggaggccc cgggtaccta gcctgtcgcc gatgactggg
     5101 gacgagctac tggccgggcg ctaccggacg ctgcgcctga tcggcaccgg cggcatggcg
     5161 cgcgtgttcc tggccgagga ccagcgcctg ggccgccagg tggcggtcaa gcggctgcac
     5221 gcgcacagcc cggaggacat cgcccgccgc ttccagcgcg aggcgcggct tggcgccgcg
     5281 ctgaaccacc cgaacctggt ggcggtgtac gacgtggaga ccgacggcga gtcggtgctc
     5341 atcgtcatgg agtacgtgga gggcaccacg ctggcggacg agctcaaggc gggcgcgctg
     5401 ggcgtggacc gcacggtcgc gatgatctcg gacgtggcgg ccgccctgga cgccgtgcac
     5461 gagaagggca tcgtgcaccg ggacgtcaag ccggccaacg tgctgctgcg cagcaaggac
     5521 gggatggcca agctggccga cctggggatc gccaccgcgg ccgagagcac gcgcatcacc
     5581 gtcagcggca ccgtgatggg tacggccgcc tacatggccc cgg
//
LOCUS       DASUPW010000012         3542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1941553, whole genome shotgun sequence.
ACCESSION   DASUPW010000012 DASUPW010000000
VERSION     DASUPW010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1941553"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="VFY44_00245"
     CDS             <1..1015
                     /locus_tag="VFY44_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908887.1"
                     /translation="LSYFGLVPAALAGVDVGHMCRSAAEAAAECRDESDANPGLAIGV
                     MLGTLALEGRNKATFVVSPRIAAFGLWIEQLIAESTGKQGRGILPVADEPLARPDFYG
                     EDRVFVYLRDADQPDSGLDGTVEDLRGAGHPVVTLEHAGPDDLGRIFFLSEFATAVAG
                     WVLDINAFDQPNVQEAKDNTNKVLEADEPPRVEEADDAALSSLLGTAAPPHYVAIMGY
                     VPPTEEFDDAVLDLRRTIRDATRATTTFGYGPRFLHSTGQFHKGGPPEGLFLQLTHEP
                     GDDVPIPDADFGFRELIAAQATGDVLTLRDHGLAAERVVLEGDPAQAVRALTEKVREM
                     LDD"
     gene            1041..1991
                     /gene="gnd"
                     /locus_tag="VFY44_00250"
     CDS             1041..1991
                     /gene="gnd"
                     /locus_tag="VFY44_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEX5908888.1"
                     /translation="MQVGFVGLGKMGGNMVHRIHRDSDHTVVAFDFSEDAVKKTEEYG
                     ATGASSLEDLVSKLDAPRAVWIMVPAGDATQETVDALADLLDEGDAIIDGGNSRWTDD
                     KVRAKALAEKGIEYADVGTSGGVWGLEVGYCMMVGGTEEAVERLAPILDVLAPPETEE
                     HGPGWQHFGGPGAGHYVKMVHNGVEYGLMQAYAEGFDIFDKSEYDLDKAKIAHLWMQG
                     SVVRSWLCELSALAFEQEGNDLGDLDAYTEDSGEGRWTIQDATDKDVPTPVITAALYT
                     RFYSRGNGEFSEKMLSALRNQFGGHSVRTRAQQASDPSDV"
     gene            1991..>3542
                     /gene="zwf"
                     /locus_tag="VFY44_00255"
     CDS             1991..>3542
                     /gene="zwf"
                     /locus_tag="VFY44_00255"
                     /inference="COORDINATES: protein motif:HMM:NF009492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX5908889.1"
                     /translation="MATEPGHAEHGAHAEENPLAEGLERQPVHPTTLTIFGATGDLSK
                     RKLLPALYNLAHEGALPERFNLIGSSRSDLSADDFRELATDSINEFSRREPDPKVLEA
                     LLSDVQYVSGAFDEDDVYEQLKEAASTFDEEAGITFNRVFYLSTAPSFFPVIVQQLGN
                     HGLDHHEDSDVRVVIEKPIGRNLQEAKDLNRAVLDVLDEDQVFRIDHYLGKETVQNML
                     AFRFANGLFEPIWNRNYVDNVQITSSEDIGIGSRAGYYDTSGALRDLVQNHMLQLLCL
                     LCMEPPVNFYADEVRDEKVKVLHAIHPPKPEEVKDMAVRAQYTAGVAAGEDVKGYKDE
                     DDVPEDSVTETYAALRLEVDNWRWAGVPIYLRTGKRLARKVTEIAVTLKPVPHLAFQA
                     SGSVGVQPNQIVMTMQPNEGVSISLGAKIPGSTMRIRPVNREFLYGTAFMSESPEAYE
                     RLIMDAMRGEATLFTRDDEVEAQWAICDPILEAWEADDAPLYEYEAGSSGPEEVDQLL
                     VHDERWRTI"
BASE COUNT          588 a         1223 c         1251 g          480 t
ORIGIN      
        1 cctctcctac ttcggcctcg tccccgccgc gctggccggc gtggacgtgg gccacatgtg
       61 ccgttcggcg gccgaggcgg cggccgagtg ccgtgacgag agcgacgcca accccggcct
      121 ggccatcggc gtcatgctgg gcacgctggc cctggagggc cgcaacaagg ccacgttcgt
      181 ggtctcgccg cggatcgccg ccttcgggct gtggatcgag cagctcatcg ccgagtccac
      241 cggcaagcag ggccgcggga tcctgcccgt ggccgacgag ccgctcgcgc ggcccgactt
      301 ctacggcgag gaccgcgtgt tcgtctacct gcgcgacgcc gaccagcccg actcgggcct
      361 ggacggcacg gtcgaggacc tgcgtggcgc gggccacccc gtggtcacgc tcgagcacgc
      421 cggcccggac gacctggggc ggatcttctt cctgtccgag ttcgccaccg ccgtggccgg
      481 ctgggtgctg gacatcaacg cgttcgacca gcccaacgtg caggaggcca aggacaacac
      541 caacaaggtg ctggaggccg acgagccgcc ccgcgtcgag gaggccgacg acgccgcgct
      601 gtcgtcgctg ctgggcaccg ccgcgccgcc ccactacgtg gcgatcatgg gctacgtgcc
      661 gccgaccgag gagttcgacg acgccgtcct ggacctgcgc cgcaccatcc gcgacgccac
      721 ccgggccacc accacgttcg gctacggccc gcgcttcctg cactccaccg gccagttcca
      781 caagggcggg ccgcccgagg ggctgttcct gcagctgacg cacgagccgg gcgacgacgt
      841 gccgatcccc gacgccgact tcggcttcag ggagctgatc gcggcgcagg ccaccggcga
      901 cgtgctgacc ctgcgcgacc acggcctggc ggccgagcgc gtggtgctgg agggcgaccc
      961 ggcgcaggcc gtgcgggcgc tgacggagaa ggtcagggaa atgctggacg actgatgacc
     1021 ctcgcgattg gaggcagcgc atgcaggtcg gcttcgtagg gctgggaaag atgggcggca
     1081 acatggtcca tcgcatccac cgcgactcgg accacacggt cgtcgcgttc gacttctccg
     1141 aggacgcggt gaagaagacc gaggagtacg gcgccaccgg cgcgtcctcg ctggaggacc
     1201 tggtctccaa gctcgacgcg ccccgggcgg tgtggatcat ggtccccgcc ggcgacgcga
     1261 cccaggagac ggtggacgcc ctggccgacc tgctggacga gggcgacgcc atcatcgacg
     1321 gcggcaactc gcgctggacc gacgacaagg tccgcgcgaa ggcgctggcc gagaagggca
     1381 tcgagtacgc cgacgtgggc accagcggcg gcgtctgggg gctcgaggtc ggctactgca
     1441 tgatggtcgg cggcaccgag gaggccgtcg agcggctggc cccgatcctg gacgtgctgg
     1501 ccccgcccga gaccgaggag cacggccccg gctggcagca cttcggcggc cccggcgccg
     1561 gccactacgt gaagatggtg cacaacggcg tcgagtacgg cctgatgcag gcctacgccg
     1621 agggcttcga catcttcgac aagtccgagt acgacctgga caaggcgaag atcgcccacc
     1681 tgtggatgca gggctcggtg gtgcgcagct ggctgtgcga gctgtccgcg ctggccttcg
     1741 agcaggaggg caacgacctg ggcgacctgg acgcgtacac cgaggactcc ggcgagggcc
     1801 gctggacgat ccaggacgcg accgacaagg acgtgcccac cccggtgatc accgccgccc
     1861 tctatacgcg cttctactcg cgcggcaacg gtgagttctc cgagaagatg ctgtcggccc
     1921 tgcgcaacca gttcggcggc cactcggtgc gtacccgtgc gcagcaggcc tccgacccct
     1981 ccgacgtctg atggccaccg agcccgggca cgccgagcac ggcgcgcacg cagaggagaa
     2041 cccgctggcc gagggcctgg agcgccagcc ggtgcacccg accaccctga cgatcttcgg
     2101 cgccaccggt gacctgtcca agcgcaagct gttgccggcg ctctacaacc tggcccacga
     2161 gggcgcgctg ccggagcgct tcaacctgat cggcagctcg cgcagcgacc tgtcggcgga
     2221 cgacttccgg gagctggcca ccgactcgat caacgagttc tcgcgccgtg agcccgatcc
     2281 gaaggtgctg gaggcgctgc tgtccgacgt gcagtacgtc tcgggcgcgt tcgacgagga
     2341 cgacgtctac gagcagctga aggaggccgc gtccacgttc gacgaggagg cggggatcac
     2401 gttcaaccgc gtcttctacc tgtccacggc gcccagcttc ttccccgtga tcgtccagca
     2461 gctgggcaac cacgggctcg accaccacga ggactccgac gtccgggtgg tcatcgagaa
     2521 gccgatcggg cgcaacctcc aggaggccaa ggacctgaac cgggccgtgc tcgacgtgct
     2581 ggacgaggac caggtgttcc ggatcgacca ctacctgggc aaggagaccg tccagaacat
     2641 gctggcgttc cggttcgcca acggcctgtt cgagcccatc tggaaccgca actacgtgga
     2701 caacgtgcag atcacgtcgt ccgaggacat cggcatcggc agccgtgcgg gctactacga
     2761 cacctccggg gcgctgcgcg acctggtgca gaaccacatg ctgcagctgc tgtgcctgct
     2821 gtgcatggag ccgccggtga acttctacgc cgacgaggtc cgcgacgaga aggtcaaggt
     2881 cctgcacgcc atccacccgc cgaagcccga agaggtcaag gacatggccg tgcgcgccca
     2941 gtacacggcc ggcgtggccg cgggggagga cgtcaagggc tacaaggacg aggacgacgt
     3001 ccccgaggac tcggtcaccg agacctacgc ggcgctgcgg ctggaggtgg acaactggcg
     3061 ctgggcgggc gtgccgatct acctgcgcac cggcaagcgg ctggcgcgga aggtcaccga
     3121 gatcgcggtg acgctcaagc cggtgccgca cctggccttc caggccagcg gctcggtggg
     3181 ggtgcagccc aaccagatcg tcatgaccat gcagcccaac gagggcgtgt cgatctcgct
     3241 gggggcgaag atccccggca gcacgatgcg gatccggccg gtgaacaggg agttcctgta
     3301 cgggaccgcg ttcatgtcgg agtcccccga ggcctacgag cggctgatca tggacgccat
     3361 gcgcggcgag gcgacgctgt tcacgcgcga cgacgaggtc gaggcccagt gggccatctg
     3421 cgacccgatc ctggaggcgt gggaggccga cgacgcgccg ctgtacgagt acgaggcggg
     3481 ctccagcggt cccgaggagg tcgaccagct gctcgtgcac gacgagaggt ggcggacgat
     3541 tt
//
LOCUS       DASUPW010000013         2121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_279310, whole genome shotgun sequence.
ACCESSION   DASUPW010000013 DASUPW010000000
VERSION     DASUPW010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279310"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1915
                     /locus_tag="VFY44_00260"
     CDS             <1..1915
                     /locus_tag="VFY44_00260"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5908890.1"
                     /translation="LDGATRGRSASLLGFGEPGIGKSALAAQAVEMALDRGMRVLRAR
                     GVEAETDIPFAGLSELLAPVLDHRRQLPPVQAEALGAALALEPAAGAHDRFAVAAALL
                     SLLAAAADEQPLLAVVDDAQWVDDASLDSLLFAARRLENEGVVILLGTRPEPDRGLLG
                     RGMFEFELQPLGDQDARRLLDRAAGEPLADDAADRLLRSAAGNPLALLELPGAPQADT
                     AGAPGPPAPGARVEAAFRATLAQLPPATREALTVVAADEASAPVTVAAALDQLGLPPD
                     ALAAAERARVIQVTRGQLQFRHPLLRSVAYHQAGAEQRRRVHAALAVATGQDDDPVAR
                     AWHLAAAATGRDRRVADELEGAAASARARGAVTSAAHALSRSAELTPDPTDRARRLLA
                     AARDWASTERPAESAELAAQALELTDEPLLRADLQRVRGEVMIRMGQFDAAHDLLTVA
                     AEQVAPLDERRAAEMYLAASVRHRASGSYGQMRRDAERARELAGLDHPDVARFADLVL
                     AHTLVVSGVRLRGDALIGLHEEALVSALARDEVNIELLTSPAHASMWAGRRQRAERIA
                     RLLVGRARQSSAPGALIFPLGIQSQLHYRAGRWRAAYAGAEEASGWPPTPASWPGGPT
                     PPACWPRSRRPWG"
     gene            1882..>2121
                     /locus_tag="VFY44_00265"
     CDS             1882..>2121
                     /locus_tag="VFY44_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908891.1"
                     /translation="MLAEIEASMGLIEQARASAARAVAITDPSDAAAIGLYARAALGL
                     AELGQGRAAEAVDTLQWCAESSRRLGLSEPNVHRWA"
BASE COUNT          222 a          859 c          816 g          224 t
ORIGIN      
        1 gctggacggc gcgacgcgtg gccggagcgc ctcgctgctg ggcttcggcg agcccggcat
       61 cggcaagtcc gcactggccg cccaggccgt ggagatggcc ctggaccggg gcatgcgggt
      121 gctgcgcgcc cgcggcgtcg aggccgagac cgacatcccc ttcgccggcc tgtccgagct
      181 gctggccccg gtgctggacc accgccgcca gctcccgccc gtccaggccg aggcgctggg
      241 cgcggcgctg gcgctggagc cggccgccgg cgcgcacgac cgcttcgccg tggccgcggc
      301 gctgctgagc ctcctggccg ccgccgccga cgagcaaccc ctgcttgccg tggtcgacga
      361 cgcgcagtgg gtcgacgacg cctccctgga ctcgcttctg ttcgccgccc gccggctgga
      421 gaacgagggc gtggtcatcc tcctgggcac ccgccccgag cccgatcgcg ggctcctggg
      481 ccgcggcatg ttcgagttcg agctccagcc gctgggcgac caggacgcgc gccggctgct
      541 ggaccgcgcg gccggcgagc cgctggccga cgacgcggcc gaccggctgc tgcgctcggc
      601 ggccggcaac cccctggcgc tgctcgagct gccgggcgcg ccgcaggccg acaccgccgg
      661 cgcccccggc ccgcccgcgc ccggggcccg ggtggaggcc gccttccgcg cgacgctggc
      721 ccagctgccc cccgccaccc gcgaggccct gaccgtcgtg gccgccgacg aggcgtccgc
      781 gccggtcacg gtggcggcgg ccctggacca gctcggcctg cccccggacg cgctcgcggc
      841 ggccgagcgc gcccgcgtga tccaggtgac gcgcggccag ctgcagttcc gccaccccct
      901 gctccggtcg gtggcctacc accaggccgg agcggagcag cgccggcgcg tgcacgcggc
      961 gctggccgtg gccaccggcc aggacgacga ccccgtggcg cgggcgtggc acctggccgc
     1021 ggccgccacg ggccgcgacc ggcgcgtggc cgacgagctg gagggcgccg ccgcctccgc
     1081 ccgggcgcgc ggcgccgtga cgtcggcggc ccacgccctg agccggtccg cggagctgac
     1141 gcccgacccc accgaccgcg cccgccggct gctggccgcc gcccgcgact gggcctccac
     1201 cgagcggccc gccgagagcg ccgagctggc cgcccaggcg ctggagctga ccgacgagcc
     1261 gctgctgcgt gccgacctcc agcgcgtgcg cggcgaggtg atgatccgca tgggccagtt
     1321 cgacgccgcg cacgacctgc tgaccgtggc cgccgagcag gtcgcgcccc tggacgagcg
     1381 ccgggcggcg gagatgtacc tggccgccag cgtgcggcac cgggcgtcgg gcagctacgg
     1441 ccagatgcgc cgtgacgccg agcgggcgcg cgagctggcc gggctggacc accccgacgt
     1501 ggcccgcttc gccgacctgg tcctggccca cacgctcgtg gtctcaggag tgcggctgcg
     1561 gggcgacgcg ctgatcggcc tgcacgagga ggcgctggtc tcggccctgg cccgggacga
     1621 ggtgaacatc gagctgctga cctcaccggc ccacgcgtcc atgtgggccg ggcggcgcca
     1681 gcgcgccgag cggatcgccc ggctgctggt cggccgcgcc cgtcagagca gcgcaccagg
     1741 cgccctgatc ttcccgctgg ggatccagtc ccagctgcac taccgggccg gccgctggcg
     1801 cgcggcgtac gccggcgccg aggaggcgtc cggctggccg ccgacaccag ccagctggcc
     1861 cggggggcct acaccaccgg cgtgctggcc gagatcgagg cgtccatggg gctgatcgag
     1921 caggcgcgcg ccagcgccgc ccgggcggtc gccatcaccg acccctccga cgcggcggcc
     1981 atcggcctgt acgcgcgcgc cgccctgggc ctggccgagc tgggccaggg acgggcggcc
     2041 gaggcggtgg acacgctcca gtggtgcgcc gagagctcgc gccggctcgg cctgagcgag
     2101 cccaacgtgc accgctgggc c
//
LOCUS       DASUPW010000014         3804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_833570, whole genome shotgun sequence.
ACCESSION   DASUPW010000014 DASUPW010000000
VERSION     DASUPW010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833570"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VFY44_00270"
     CDS             <1..453
                     /locus_tag="VFY44_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908892.1"
                     /translation="AAASRVRDIVGNGALTRLARRARMRRFATAPSWGATRICTADQT
                     RFFLGLDRFVVPRHRATALRLLGSVVPSQRWGIAKVRPPGWKLYFKGGWGDYGERDHQ
                     MALLTRAGRRLAVGIMVTGTPSKAYGDRTLEGVSRRLLRGLDADAAPR"
     gene            477..1919
                     /locus_tag="VFY44_00275"
     CDS             477..1919
                     /locus_tag="VFY44_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX5908893.1"
                     /translation="MRGRVLATAIAAIAIAGTGGSAAHANQVIEYEIPARGGEVAGGY
                     NYAGPPRARAILPDGYDPKRSYPVLYVLHGFSCKYSCWTNPKGIAIDKHLPGLDAIVV
                     MPEGGTGGWYTDAWNGGRRGNPAWESYFLDQVIPQTEARFRIRPERRYHALAGSSMGG
                     LGTAYLGGRLPGYFGSLVVMSGFVDTQIIPAVGFIQSLMAGGRPEDLYGPLDGPYATG
                     HNPARLAGNLTRTRVLVSHGTGAPQGGDTGTPGEGVTEGAFIQPMSLSYVAALRRARV
                     DVTHLSHPGYHDAANDRRQMRDALGWGLFQPVPERPATWTNDTVAGRGKLWDLRYRFD
                     SPPTRVARFSRDGRRLQITAAGSPVTLTTDGGCVIRTATPGAVDIPARPCARLTVRVR
                     PRRLRTGRMTRVRIAVRPAASGVLVRSGRRRARTSSRGVAYLRVCARTRRGVKVAAGA
                     PNFLDAGVRVRARGRAASCRRAAVRKRRAK"
     gene            1932..3068
                     /locus_tag="VFY44_00280"
     CDS             1932..3068
                     /locus_tag="VFY44_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5908894.1"
                     /translation="MDLTLSPNEQAFRDEVRGWLAENHPGEAPRGDDASFDFLKQWQL
                     KLHEQGYAGLSWPEEYGGRGATLIEQAIFGEELARAKAPGPANVLGLVMGGPVVIAHG
                     SEEQKQRYLEPILSGEEIWCQGFSEPEAGSDLASLKTKAVKDGDEWVVTGQKVWTTLA
                     HEAKWCMLVARTDPDAPKHKGLTYFLMDMEQDAVQINPLHQITGEAEFNELFIEEARI
                     PDANVVGGEGNGWMVAITTLMNERAGLGAGAAATMKIALGQLIELAKERGVDDDPLVR
                     QKIASLLIEVEALRLNAWRGLTAIMKTGIPGPEGSLPKLQWSAANQGLTELAMEIRGE
                     EAAVKDSDWTYRFLRARANSIEGGTTEILKNIIAERVLNLPKLR"
     gene            3065..>3804
                     /locus_tag="VFY44_00285"
     CDS             3065..>3804
                     /locus_tag="VFY44_00285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5908895.1"
                     /translation="MNFDYSDDQQAIKRTAHDLLVARFKPDTVRELAEGERYDQAHWD
                     ELCELGWPGIFIDEEYDGQGLGTVELVILLEELGYTLAPVPFLSNAAAGLALQHAGSD
                     EQKDAHLKGIATGAERGTVGTLKNGVAELVPDADTAKVIVFLDGDEATLHLAKDCDIQ
                     PVDAIDQTRRYSRVVPGGNGEILPGDVTGASDRMAVAVSAELVGASQRVMEMAVEYAR
                     DRKQFDRPIGAYQAVAHRCADMLRETESA"
BASE COUNT          564 a         1366 c         1401 g          473 t
ORIGIN      
        1 gcggcggcgt cgcgggtgcg cgacatcgtg ggcaacggcg cgctgacgcg gctggcgcgg
       61 cgagcgcgga tgcgccgctt cgccaccgcg ccgtcgtggg gtgccacccg catctgcacc
      121 gccgaccaga cgcgcttctt cctgggcctg gaccggttcg tggttccccg ccaccgagcc
      181 accgccctcc gcctgctggg ctcggtcgtg ccctcgcagc gctggggcat cgccaaggtg
      241 cggccgcccg gctggaagct ctacttcaag ggcggctggg gcgactacgg cgagcgcgac
      301 caccagatgg ccctgctgac ccgcgccggc cggcggctgg ccgtgggcat catggtcacc
      361 ggcaccccgt ccaaggccta cggcgaccgg acgctggagg gggtcagccg gcggctgctg
      421 cgcgggctgg acgccgatgc cgcgccgcgc tgagcggccg atatagcatc gcgcggatga
      481 ggggcagggt tctcgccacg gcgatcgcag cgatcgccat cgccggcacg ggcggctccg
      541 ccgcgcacgc caaccaggtg atcgagtacg agatccccgc gcgtggcggt gaggtggccg
      601 gcggctacaa ctacgcgggg cctccgcggg cacgcgcgat cctgcccgac gggtacgacc
      661 cgaagcggtc ctaccccgtc ctctacgtcc tccacggctt ctcgtgcaag tactcgtgct
      721 ggacgaaccc gaaggggatc gcgatcgaca agcacctccc ggggctcgac gcgatcgtcg
      781 tgatgcccga ggggggcacc ggcggctggt acacggacgc gtggaacggc ggccgccggg
      841 ggaacccggc gtgggagagc tacttcctcg accaggtgat cccgcagacc gaggcgcggt
      901 tcaggatccg gccggagcgc cgctaccacg ccctcgccgg ctcgtcgatg ggcgggctgg
      961 ggacggcgta cctgggtggg cggctgcccg gctacttcgg atcgctggtc gtgatgtccg
     1021 gcttcgtcga cacgcagatc atcccggccg tgggcttcat ccagtcgctg atggccggag
     1081 gccggcccga ggacctctac ggaccgctcg acgggcccta cgcgaccggg cacaacccgg
     1141 cgcggctggc gggcaacctg acccgcacgc gtgtcctggt ctcgcacggc accggcgcgc
     1201 cgcagggcgg tgacaccgga acgccgggcg agggggtcac ggagggggcg ttcatccagc
     1261 cgatgagcct ctcgtacgtg gccgcgctgc ggcgggcccg ggtcgacgtg acccatctga
     1321 gccaccccgg ctaccacgac gcggcgaacg accggcgcca gatgcgcgac gcgctcgggt
     1381 gggggctgtt ccagcccgtc ccggagcgcc ccgccacgtg gaccaacgac accgtcgccg
     1441 gccgcggcaa gctgtgggac ctcaggtacc gcttcgactc gccacccacc cgcgtcgcgc
     1501 gcttcagccg cgacggccgc cgcctccaga tcaccgcggc gggctcgccc gtgacgctca
     1561 ccaccgacgg tggctgcgtg atccgcacgg cgacgcccgg cgcggtggac atcccggcac
     1621 ggccgtgcgc gaggctcacg gtgcgggtcc ggccgcgtcg cctgcggacc gggcgcatga
     1681 cacgcgtgcg gatcgccgtg cgccccgccg catccggcgt gctcgtccgg tcgggccggc
     1741 gccgcgcgcg gacgagctcg aggggggtcg cgtacctgcg cgtgtgcgcc cggacgcggc
     1801 gtggcgtcaa ggtcgcggcc ggcgctccga acttcctcga cgccggcgtg cgggtgcgcg
     1861 cacgcggacg agcggcctcc tgccgccgcg cggcggtccg caagcgccgc gccaagtaga
     1921 gtccccgcac cgtggacctg acgctctccc ccaacgagca ggccttccgc gacgaggtgc
     1981 gcggctggct ggccgagaac cacccgggcg aggcgccgcg cggcgacgac gcgagcttcg
     2041 acttcctcaa gcagtggcag ctcaagctcc acgagcaggg ctacgccggg ctgtcctggc
     2101 ccgaggagta cggcgggcgc ggcgcgacgc tgatcgagca ggccatcttc ggcgaggagc
     2161 tggcccgggc caaggcgccc ggcccggcca acgtgctggg cctggtcatg ggcgggccgg
     2221 tggtgatcgc ccacggcagc gaggagcaga agcagcgcta cctggagccg atcctgtccg
     2281 gcgaggagat ctggtgccag ggcttctccg agcccgaggc cggctcggat ctggcctcgc
     2341 tcaagaccaa ggcggtcaag gacggcgacg agtgggtcgt gaccggccag aaggtgtgga
     2401 ccacgctggc ccacgaggcc aagtggtgca tgctcgtggc ccggaccgac cccgacgccc
     2461 ccaagcacaa ggggctgacc tacttcctga tggacatgga gcaggacgcg gtgcagatca
     2521 acccgctgca ccagatcacc ggcgaggccg agttcaacga gctgttcatc gaggaggcgc
     2581 ggatcccgga cgccaacgtg gtcggcggcg agggcaacgg ctggatggtg gccatcacca
     2641 cgctgatgaa cgagcgcgcc ggcctcggcg ccggcgccgc ggccacgatg aagatcgcgc
     2701 tgggccagct gatcgagctg gccaaggagc gcggggtgga cgacgacccg ctggtgcggc
     2761 agaagatcgc ctcgctgctg atcgaggtcg aggccctgcg cctgaacgcc tggcgcgggc
     2821 tgaccgcgat catgaagacc ggcatccccg gccccgaggg ctcgctgccc aagctgcagt
     2881 ggtcggccgc caaccagggc ctgaccgagc tggccatgga gatccgcggc gaggaggcgg
     2941 cggtcaagga ctccgactgg acctaccgct tcctgcgcgc ccgggccaac tcgatcgagg
     3001 gcggcacgac cgagatcctc aagaacatca tcgccgagcg cgtcctgaac ctgcccaagc
     3061 tccgatgaac ttcgactact ccgacgacca gcaggccatc aagcgcaccg cgcacgacct
     3121 gctggtcgcg cgcttcaagc ccgacacggt gcgcgagctg gccgagggcg agcgctacga
     3181 ccaggcgcac tgggacgagc tgtgcgagct gggctggccc gggatcttca tcgacgagga
     3241 gtacgacggc cagggcctcg gcaccgtcga gctggtgatc ctgctcgagg agctgggcta
     3301 cacgctggcg ccggtgccgt tcctgtccaa cgcggccgcc ggccttgcgc tccagcacgc
     3361 gggcagcgac gagcagaagg acgcgcacct gaagggcata gcgaccgggg ccgagcgcgg
     3421 cacggtcggc acgctcaaga acggtgtggc cgagctggtg cccgacgccg acaccgccaa
     3481 ggtcatcgtg ttcctggacg gcgacgaggc cacgctgcat ctggccaagg actgcgacat
     3541 ccagcccgtc gacgcgatcg accagacgcg ccgctactca cgcgtggtcc ccggcgggaa
     3601 cggcgagatc ctgcccggtg acgtgaccgg cgcgtccgac cggatggccg tggccgtgtc
     3661 ggccgagctg gtgggcgcct cccagcgggt catggagatg gccgtggagt acgcccgcga
     3721 ccgcaagcag ttcgaccgcc cgatcggcgc ctaccaggcc gtggcgcacc gctgcgcgga
     3781 catgctgcgc gagaccgaga gcgc
//
LOCUS       DASUPW010000015         2262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1942304, whole genome shotgun sequence.
ACCESSION   DASUPW010000015 DASUPW010000000
VERSION     DASUPW010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1942304"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..642
                     /locus_tag="VFY44_00290"
     CDS             109..642
                     /locus_tag="VFY44_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908896.1"
                     /translation="MSRDELSLFSYEILGLVGGQGLGAHDLLRMARESRIMAWAGESQ
                     YYVEPKRLAKLGYLEARKEPGKTRERTVYSLTEQGLEALREYAATPVAFPAVKSDPLQ
                     RLLIADLVGEEVTRSSMATLREDVIDLQERIKEMEERAEALPHRRKYLLIVVAHLRRL
                     LAAHLELVDDVERQLAP"
     gene            652..1425
                     /locus_tag="VFY44_00295"
     CDS             652..1425
                     /locus_tag="VFY44_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEX5908897.1"
                     /translation="MRQIKSWLMDMDGVLVHENQALPGAAEFIGRLDELRIPFLVLTN
                     NSIYTARDLAARLHASGLDIPERSIWTSALATARFLSDQRPEGSAFVVGEAGLTTALH
                     TAGYTLTENAPDYVVLGETRTYSFERITKAIRLIGDGARFIATNPDATGPSGDGPLPA
                     TGSVAALISRATGVEPYFVGKPNPLMMRSALNALDAHSEETAMIGDRMDTDIVAGLEA
                     GLETVLVLTGVTREGEEERFPYRPSRILPGVGDLVADLG"
     gene            complement(1429..1929)
                     /locus_tag="VFY44_00300"
     CDS             complement(1429..1929)
                     /locus_tag="VFY44_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908898.1"
                     /translation="MITPALGIVTVVISAQTKKASAAFVGAVVLASGAYAVGSTSGDG
                     SATANQSGQSGQPGPYGAPGNGPPPQMRRQFRHDRLEIFSGLAKELGVKEADLRKAMR
                     EIRKEQRDDFVKKLADKLGVSEAKVKKALPDRPKMTRRFRGPHGAPGAPPAGPPPGAP
                     PAPYGP"
     gene            1975..2202
                     /locus_tag="VFY44_00305"
     CDS             1975..2202
                     /locus_tag="VFY44_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908899.1"
                     /translation="MADPRTPQLYCPAGLAARTSRRTAWLVAAAIAAVLATIAVATPW
                     GARGALIAFAAVWGLWMVLMPVAVIASAVRR"
BASE COUNT          323 a          806 c          805 g          328 t
ORIGIN      
        1 accggctccg acgatcagga cgggcacgct cggcggggtg tgggaaggca acgtctgctc
       61 caagcactag acgacgtttc atagttactt acactatgga acatagctat gtcaagagat
      121 gagctcagcc tgttctcgta cgagatcctc ggcctggtcg gcggacaggg gctgggcgcg
      181 cacgacctgc tgcggatggc gcgcgagtcg cggatcatgg cctgggcggg cgagagccag
      241 tactacgtgg agcccaagcg gctggccaag ctgggctacc tcgaggcgcg caaggagccg
      301 ggcaagacgc gcgagcgcac ggtctactcg ctgaccgagc agggcctcga ggcgctgcgc
      361 gagtacgccg ccacgccggt cgccttcccg gccgtgaaga gcgaccccct ccagcggctc
      421 ctgatcgccg acctggtcgg cgaggaggtc acgcgctcga gcatggccac cctgcgtgag
      481 gacgtgatcg acctgcagga gcggatcaag gagatggagg agcgcgccga ggcgctgccc
      541 caccggcgca agtacctgct gatcgtggtc gcgcacctgc gccggctgct ggccgcccac
      601 ctggagctgg tcgacgacgt cgagcgccag ctggcccctt aagctcggcc catgcgccag
      661 atcaagtcgt ggctgatgga catggacggc gtgctcgtgc acgagaacca ggcactgccc
      721 ggcgccgccg agttcatcgg ccgcctggac gagctgcgga tcccgttcct ggtgctgacc
      781 aacaactcca tctacacggc ccgcgacctg gccgcgcggc tgcacgccag cggcctggac
      841 atcccggagc gctcgatctg gacctccgcc ctggccaccg cccgcttcct gagcgaccag
      901 cgccccgagg gatcggcctt cgtggtcggc gaggccgggc tgaccaccgc cctgcacacg
      961 gcgggctaca cgctgaccga gaacgcgccc gactacgtcg tcctcggtga gacgcgcacc
     1021 tacagcttcg agcgcatcac caaggccatc cggctgatcg gcgacggggc ccgcttcatc
     1081 gccaccaacc cggacgccac ggggccgtcc ggcgacggcc cgctgcccgc cacggggtcg
     1141 gtggccgcgc tgatcagccg cgcgaccggg gtcgagccct acttcgtggg caagcccaac
     1201 ccgctgatga tgcgctcggc gctgaacgcg ctggacgccc actccgagga gacggccatg
     1261 atcggcgacc ggatggacac cgacatcgtg gccgggttgg aagccggcct ggagacggtg
     1321 ctggtgctca ccggcgtgac ccgggagggc gaggaggagc ggtttcccta ccgcccctcc
     1381 cgcatcctgc cgggggtcgg cgacctggtc gccgacctgg gctgagccct acgggccgta
     1441 cggcgcgggc ggagccccgg gcggcggacc ggcggggggc gcgccggggg cgccgtgcgg
     1501 gcccctgaag cggcgggtca tcttcggccg gtcgggcagg gccttcttga ccttggcctc
     1561 ggacacgccg agcttgtcgg ccagcttctt gacgaagtcg tcgcgctgct ccttgcggat
     1621 ctcccgcatg gccttgcgca ggtccgcctc cttcacgccc agctccttgg ccaggccgga
     1681 gaagatctcc agccggtcgt ggcggaactg gcgccgcatc tggggtggcg ggccgttgcc
     1741 cggcgcgccg tacgggccgg gctgccccga ctggccggac tggttcgccg tggccgagcc
     1801 gtcgccgctg gtgctgccga ccgcgtacgc gccggaggcc agcaccactg ctcccacgaa
     1861 cgccgctgat gccttctttg tctgtgcgct gatcacgacc gtcacgatgc ccagcgcggg
     1921 tgtgatcaac gtaagagccg atccgatcgc gccccggcgg cgtaggaacc ggtgatggcc
     1981 gatccgcgga caccgcagct gtactgcccc gcggggctgg cggcgcgcac ctcgcgccgg
     2041 accgcatggc tggtggcggc cgcgatcgcg gccgtgctgg ccaccatcgc cgtggccacg
     2101 ccgtggggcg cgcgcggcgc gctgatcgcc ttcgcggccg tctggggcct ctggatggtg
     2161 ctcatgccgg tcgccgtcat cgcctccgca gtgcgccgct gaaccgaaac cccgggaaac
     2221 cgggggttgg acccgatgta cattcgcggc ggcccaagtt tg
//
LOCUS       DASUPW010000016         6849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_972714, whole genome shotgun sequence.
ACCESSION   DASUPW010000016 DASUPW010000000
VERSION     DASUPW010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6849
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972714"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..750
                     /locus_tag="VFY44_00310"
     CDS             145..750
                     /locus_tag="VFY44_00310"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006822499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX5908900.1"
                     /translation="MAYQVPDLKYPYDALEPYIDEATMRVHHDKHHQAYVDKANGALE
                     GTEWADKPIEEVVANLSQLPDDKRGPVRNNGGGHYNHSLFWESLSPDGGGEPDGDLAS
                     AIDSAFGSFDDFKDKVKETGVGQFGSGWAWLVHDGSGLAVVGTPNQDNPITDGKTPLL
                     GIDVWEHAYYLKYQNKRPDYIDAIWNVVDWGKVAERYNAAS"
     gene            802..1251
                     /locus_tag="VFY44_00315"
     CDS             802..1251
                     /locus_tag="VFY44_00315"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX5908901.1"
                     /translation="MSATATDWTDHTRQTLAAAGHRASAPRAEVVDAIAELGCSVSAR
                     EIEDLLRERDSTVGLASIYRALELLERLGLVKRIDLGDTAARYEAASPGGEHHHHIVC
                     DSCGKVERFEDDALERAIHSLSDRSKFSVATHDVTLHGECPTCSTAG"
     gene            complement(1280..1594)
                     /gene="rpsR"
                     /locus_tag="VFY44_00320"
     CDS             complement(1280..1594)
                     /gene="rpsR"
                     /locus_tag="VFY44_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX5908902.1"
                     /translation="MARATKEKSRARKPGFKEARRYSKLPREEIDKIDYKNVALLQRF
                     ITERGKIRSRRVTGLSRRDQSLMAKAVKRSREVGLLPYVDATKAVERTGGRGGSRGRD
                     RD"
     gene            1682..3619
                     /locus_tag="VFY44_00325"
     CDS             1682..3619
                     /locus_tag="VFY44_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5908903.1"
                     /translation="MATAVLAMALMAGPAHASVTATPWPPATGQGVLFAHYGEEHWND
                     ADGLTLLPKIVEESGRYKPALVTMSGDKDNDGTVEQLSRWKQIMGPYDKQGIPFFPSV
                     GNHDRKAPPGAPPGTAGLLTPGVQGDLANYRSVFADRPYPFGDAAPYKDPGFTQRARA
                     GDDPPGASSHYFVDYKDIRVIFLDNSCWGLADCDPSQNPGFPDAQGNRSQLDFLRRNG
                     GDASRAGRQVFVVMHMPTRDPRDQSYIDPTTFNHVMGKGISPTGAQDNIRFEEVAEQA
                     GVDGVLLGHIKGQFLYEGRGKVPYFIDGGAGGELYTDGPVGTDHGYWHGFRLLRVNGG
                     RLTTDTVPIFVKDGIRLDGPGYLVQGASAHYEAFGAQPVFNDEAKVTGLELRDPDPIR
                     PPRSSGLGSVGDFVRGGGWIFVPVLLLTLGGMAMNGTLPRPRRRAAVAAAGVAGAGVI
                     AVAGVSMAQQGSPTTTPVTSLPVPARMFATTDPLVVAPVASATDDPRRDPVRQTEDGG
                     FRARCPGAARVSITSGFETTSKAVRVASRRGRIVRAARAYRSRSLRPGRRVRVSKVRL
                     GQPARVLVRIRRKGRTVRVLRDSCLSPGSKLLTSRWDGRVTRRGKLRRASAGRYRAQV
                     LVRSDRKTVSRSAVVVVRKRR"
     gene            complement(3616..4146)
                     /locus_tag="VFY44_00330"
     CDS             complement(3616..4146)
                     /locus_tag="VFY44_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5908904.1"
                     /translation="MGCLDIEQAARASGLSARDVRLLIERGKLPATRPAGRWLIDQRH
                     LAAATPRPEVDGARPIGADDTPQDTGLLLARLEEAQAQVADLRAERDELRLRLREQVS
                     SLQHSLEEAMDELDDTKARLAELQSRRPRAVPDPEPEPVRPPEPSSGLRARDALTPLF
                     RATVQPRDVGSPADRD"
     gene            complement(4101..6077)
                     /locus_tag="VFY44_00335"
     CDS             complement(4101..6077)
                     /locus_tag="VFY44_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5908905.1"
                     /translation="MVAALVVVTLAVVAGRSSDGDAEIERAAQSQAEVMRGAMGQATL
                     ARAVSQRNPSRAQRARVNRLLGRVAAESEALAVRLVGPSGRVAYSSIAGERGGKLPAT
                     PDFRMAMTGDAAAERQQADGTTSVGAYVPLRRSPGSAPAGVLNTDTPVSALEGAAPGG
                     QRQMSALLVVGVFLLVATAGGLLVSRIRARPRRKHAHSPGMSDSLTGLPNREVFNSLT
                     EKALADCPQGGKVAVLLMDLDRFKEINDSLGHFNGDLVIERVGKRLRTVLREGDQVAR
                     LGGDEFAMLLPNVQAKDSVSGAAQRIQEALSEPFSVGGLALKVEASIGSALYPTDVDK
                     AGKLLQAADVAMYAAKKAHSGHEFYSPDQHQYTPARLGLVAQLNRAMEGGELVLHYQP
                     KARLATGTVEGVEALARWNHPQRGLLFPDEFIPVTEHTSMIRPLTVHFLETALEQVDN
                     WSKQGIELNVAVNLSAQLLLDLQLPREIGRMLSRVGAASSKLEVEITESAIMSDPRRA
                     RRVLDRMREMGIRVAIDDFGTGYSSLVSLKQLPVSTIKVDKSFVMNMEADPDDAAIVR
                     STVALGHNLGLEVVAEGVETPGAWRELQAMGADIAQGYYLSKALPADQFNRWLSAYQD
                     MFGRAPERGSQANGEAGGQSWDVSTSSRPPAPAG"
     gene            complement(6198..>6849)
                     /locus_tag="VFY44_00340"
     CDS             complement(6198..>6849)
                     /locus_tag="VFY44_00340"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEX5908906.1"
                     /translation="EKLLTSVGYKIGDGPARYVLEGSIAVTGALIQWLRDRLKIIDSA
                     PAVEELAKTVDDNGGVYFVPAFSGLFAPHWRDDARGVICGLTAYSNQGHIARAALEAT
                     AWQSKEVVDAANDVADVPFQEMRVDGGMTANELLMQFQADVLGVPVIRPAVTETTALG
                     AAYAAGLAVGFWKDRGELKERWAEDKRWEPQMDADEREAGLAKWRKAVERSLDWEE"
BASE COUNT          940 a         2506 c         2459 g          944 t
ORIGIN      
        1 gagggcggct atctcctcga cccagctctg cagtttcttg ttttcggtag ggacggtcgt
       61 ctgggtcacg gcgtgcgagc tcctccggtg gcttggcctg ggcaggcgga gaagctagcg
      121 taacgctccc aaccaaggag accgatggcc taccaagtac cggacttgaa gtacccctac
      181 gacgccctcg agccctacat cgacgaggcg acgatgcggg tgcaccacga caagcaccac
      241 caggcctacg tcgacaaggc caacggcgcc ctggagggca ccgagtgggc cgacaagccc
      301 atcgaggagg tcgtggccaa cctctcgcag ctgcccgacg acaagcgcgg ccccgtgcgc
      361 aacaacggcg gcggccacta caaccactcc ctgttctggg agtccctgtc gcccgacggc
      421 ggcggcgagc ccgacggcga cctggcctcg gccatcgact cggccttcgg ctccttcgac
      481 gacttcaagg acaaggtcaa ggagaccggc gtgggccagt tcggctccgg ctgggcctgg
      541 ctggtgcacg acggcagcgg cctggccgtg gtcggcaccc ccaaccagga caacccgatc
      601 accgacggca agacgccgct gctggggatc gacgtgtggg agcacgccta ctacctgaag
      661 taccagaaca agcgccccga ctacatcgac gcgatctgga acgtcgtcga ctggggcaag
      721 gtcgccgagc gctacaacgc ggcctcctag agcgcatcga cgggccgggc gccatgcccg
      781 gcccgtctat cgttccctgg cgtgagcgcg accgccaccg actggaccga ccacacccgc
      841 cagaccctgg cggccgccgg ccaccgcgcc agcgcgccgc gggccgaggt ggtcgacgcg
      901 atcgccgagc tgggctgctc ggtcagcgcc agggagatcg aggacctgct gcgcgagcgc
      961 gacagcacgg tcggcctggc gtcgatctac cgcgcgctgg agctgctgga gcgcctgggg
     1021 ctggtcaagc ggatcgacct cggcgacacc gccgcgcgct acgaggccgc ctcaccgggc
     1081 ggcgagcacc accaccacat cgtctgcgac agctgcggca aggtggagcg gttcgaggac
     1141 gacgccctgg agcgagccat ccactcgctg tcggaccggt cgaagttcag cgtggccacg
     1201 cacgacgtga cgctgcacgg cgagtgcccc acctgtagca ccgcgggctg aaggctccga
     1261 aaaccgagcc cggaaagtcc tagtcgcggt cgcggccgcg ggagccgcca cggccgccgg
     1321 tgcgctcgac ggccttggtg gcgtcgacgt acggcagcag gccgacctcg cgcgagcgct
     1381 tgacggcctt ggccatcagc gactggtcac ggcgcgagag cccggtcaca cggcgcgaac
     1441 ggatcttgcc gcgctcggtg atgaagcgct ggaggagagc gacgttcttg tagtcgatct
     1501 tgtcgatctc ctcgcgcggg agcttcgagt agcggcgcgc ctccttgaat ccaggcttgc
     1561 gtgcgcgcga cttttccttg gttgctcgtg ccatgcgacg gacaagggtg acagatgcga
     1621 acgagttgtg gcttgcgcgg ccgtccgcga ccggtcagtg tggcctgcga tgcggcgggc
     1681 ggtggcgacg gcggtactgg cgatggccct gatggccggc cccgcgcacg cctcggtgac
     1741 cgccacgccg tggccccccg ccaccggcca gggcgtgctg ttcgcccact acggcgagga
     1801 gcactggaac gacgccgacg gcctgaccct gctgcccaag atcgtggagg agagcgggcg
     1861 ttacaagccc gccctggtga cgatgtcggg cgacaaggac aacgacggca cggtggagca
     1921 gctgagccgc tggaagcaga tcatggggcc ctacgacaag caggggatcc cgttcttccc
     1981 gtcggtcggt aaccacgacc gcaaggcgcc gccgggcgcg ccgcccggga cggcgggcct
     2041 gctgaccccc ggcgtgcagg gcgacctggc caactaccgc agcgtcttcg ccgaccgccc
     2101 ctacccgttc ggcgacgccg ccccctacaa ggaccccggc ttcacccagc gcgcccgcgc
     2161 gggcgacgat ccgcccggcg cgtcgtcgca ctacttcgtc gactacaagg acatccgggt
     2221 gatcttcctg gacaactcgt gctggggcct ggccgactgc gaccccagcc agaaccccgg
     2281 cttccccgac gcgcagggca accgcagcca gctggacttc ctgcgccgca acggcggcga
     2341 cgccagccgc gccggccggc aggtgttcgt ggtcatgcac atgcccaccc gcgatccccg
     2401 cgaccagagc tacatcgacc ccaccacctt caatcacgtg atgggcaagg gcatcagccc
     2461 caccggcgcg caggacaaca tccgcttcga ggaggtggcc gagcaggccg gcgtcgacgg
     2521 cgtgctgctg ggccacatca agggccagtt cctgtacgag ggccgcggca aggtgcccta
     2581 cttcatcgac ggcggcgcgg gcggggagct gtacaccgac ggcccggtgg gcaccgacca
     2641 cggctactgg cacggcttcc ggctgctgcg cgtgaacggc ggcaggctga ccaccgacac
     2701 ggtcccgatc ttcgtcaagg acggcatccg cctggacggg cccggctacc tggtccaggg
     2761 cgcgagcgcc cactacgagg cgttcggcgc ccagcccgtg ttcaacgacg aggccaaggt
     2821 caccggcctg gagctgcgcg accccgaccc gatccgcccg ccccggagct cggggctggg
     2881 gtcggtgggc gacttcgtcc gcggcggtgg gtggatcttc gtgcccgtgc tgctgctgac
     2941 gctggggggc atggccatga acgggaccct cccccgcccc cgccggcgcg cagcggtggc
     3001 ggcggccggc gtggcgggcg cgggcgtgat cgccgtggcg ggcgtgtcga tggcccagca
     3061 gggctcgccc accaccaccc cggtgaccag cctgccggtg ccggcgcgca tgttcgccac
     3121 caccgacccg ctggtggtgg cgcccgtggc ctccgcgacc gacgaccccc gccgcgaccc
     3181 ggtgcgccag accgaggacg gcggcttccg cgcgcgctgc cccggcgcgg cccgcgtgag
     3241 catcacctcc ggcttcgaga ccacgtccaa ggcggtgcgg gtggccagcc gccgcgggcg
     3301 gatcgtccgc gccgcgcgcg cctaccggtc gcggtcgctg cgcccgggcc ggcgcgtgcg
     3361 ggtgtcgaag gtccgcctgg gccagcccgc ccgcgtcctg gtgcggatcc ggcgcaaggg
     3421 caggaccgtg cgcgtgctcc gggactcgtg cctgtcaccg ggcagcaagc tgctgacctc
     3481 gcgctgggac gggcgcgtga cccgccgcgg caagctccgg cgcgcgtccg cggggcgcta
     3541 ccgcgcgcag gtgctggtcc gctcggaccg caagacggtc agccgctcgg cggtcgtggt
     3601 ggtgcgaaag cgccgctagt cgcggtcggc gggcgacccg acgtcacggg gctggaccgt
     3661 ggcgcggaac agcggggtca gcgcgtcgcg cgcccgtagg ccgctcgagg gctcgggcgg
     3721 gcgcaccggc tcgggctcgg ggtccgggac cgcgcggggg cgccgggact gcagctccgc
     3781 gagcctggcc ttcgtgtcgt ccagctcgtc catcgcctcc tcgaggctgt gctggagcga
     3841 ggacacctgc tcacgcaggc gcaggcgcag ctcgtcgcgc tcggcgcgca ggtcggcgac
     3901 ctgggcctgt gcctcctcga gccgggccag caggaggccg gtgtcctggg gcgtgtcgtc
     3961 ggccccgatg ggccgtgcgc cgtcgacctc cggccggggc gtggccgcgg ccaggtggcg
     4021 ctggtcgatc agccagcggc cggccggccg cgtggccggc agcttcccgc gctcgatcaa
     4081 gaggcggacg tcgcgcgcgc tcagcccgct ggcgcgggcg gcctgctcga tgtcgagaca
     4141 tcccatgact gaccgccagc ttcgccgttc gcctgcgatc ccctctcggg agcgcgcccg
     4201 aacatgtcct ggtaggcgga cagccagcgg ttgaactggt cggccggcag cgccttgctc
     4261 aggtagtagc cctgggcgat gtccgccccc atggcctgca gctcgcgcca ggctcccggc
     4321 gtctcgacac cctcggccac gacctccaag cccaggttgt ggcccagggc cacggtcgag
     4381 cgcacgatgg cggcgtcgtc ggggtcggcc tccatgttca tgacgaagga cttgtcgacc
     4441 ttgatcgtgc tgaccggcag ctgcttgagg gacaccagcg aggagtagcc ggtgccgaag
     4501 tcgtcgatgg ccacgcggat gcccatctcg cgcatccggt ccaggacgcg gcgggcgcgg
     4561 cgcgggtcgg acatgatcgc gctctcggtg atctccacct cgagcttgga cgaggcggcc
     4621 cccacgcgcg agagcatccg gccgatctcg cgcggcagct ggaggtccag cagcagctgg
     4681 gccgagaggt tgacggccac gttcagctcg atgccctgct tgctccagtt gtccacctgc
     4741 tccagcgccg tctccaggaa gtgcacggtc agcgggcgga tcatcgaggt gtgctcggtc
     4801 acggggatga actcgtccgg gaacagcagc ccccgctggg ggtggttcca gcgcgccagg
     4861 gcctcgacgc cctcgaccgt gcccgtggcc aggcgcgcct tgggctggta gtgcagcacc
     4921 agctcgccgc cctccatggc gcggttcagc tgcgccacca gccccagccg cgcgggcgtg
     4981 tactggtgct ggtccggtga gtagaactcg tgcccgctgt gggccttctt ggccgcgtac
     5041 atggccacgt cggcggcctg caggagcttg ccggccttgt ccacgtcggt cgggtacagc
     5101 gccgagccga tgctggcctc gaccttcagc gccaggccgc cgacgctgaa cggctcgctc
     5161 agcgcctcct ggatccgctg ggccgcgccg gacaccgagt ccttggcctg cacgttgggc
     5221 agcagcatcg cgaactcgtc cccgcccagg cgcgccacct ggtcgccctc gcgcagcacg
     5281 gtgcgcaggc gcttgcccac gcgctcgatg accaggtcgc cgttgaagtg gccgagcgag
     5341 tcgttgatct ccttgaaccg gtccaggtcc atcagcagca cggcgacctt gccgccctgg
     5401 gggcagtcgg ccagggcctt ctcggtcagg gagttgaaca cctcgcggtt gggcaggccg
     5461 gtcagcgagt cggacatgcc cgggctgtgg gcgtgcttgc gccgcggccg ggcgcggatg
     5521 cggctgacca gcaggccgcc ggcggtggcc accagcagga acacgcccac gaccagcagc
     5581 gcggacatct ggcgttgccc gcccggggcg gcgccctcga gcgcgctcac gggcgtgtcg
     5641 gtgttcagga cgccggccgg cgccgagccg ggcgaccggc gcagcggcac gtacgcgccc
     5701 acggaggtcg tcccgtcggc ctgctggcgc tcggccgcgg cgtcgccggt catggccatg
     5761 cggaagtcgg gggtcgcggg cagcttgccc ccgcgctcgc cggcgatgct ggagtaggcg
     5821 acgcggccgc tggggccgac cagccggacg gccagggcct cgctctcggc ggccacgcgg
     5881 cccagcagcc ggttcacccg cgcgcgctgg gcgcgtgagg ggttgcgctg ggacaccgcg
     5941 cgggccaggg tggcctggcc catcgcgccg cgcatgacct cggcctggct ctgggcggcg
     6001 cgctcgatct cggcgtcgcc gtcgctcgag cgcccggcca ccaccgccag ggtgaccacc
     6061 accagggcgg cgaccaacag tccgatcttc atggcgagcg gcggaaggcg cggtccagac
     6121 gagggcatcc ccatccggat cggtccctgg ggcgccgaac ttgagcgccg gcgcccccgc
     6181 ctaggcgcag gtgcgggcta ctcctcccag tccagcgagc gctccaccgc cttgcgccac
     6241 ttggccaggc cggcctcgcg ctcgtcggcg tccatctgcg gctcccagcg cttgtcctcg
     6301 gcccaccgct ccttgagctc gccgcggtcc ttccagaagc cgaccgccag gccggcggcg
     6361 tacgccgcgc ccagcgcggt ggtctcggtc acggcgggcc ggatcaccgg cacgcccagg
     6421 acgtcggcct ggaactgcat cagcagctcg ttggccgtca tgccgccgtc gacccgcatc
     6481 tcctggaagg gcacgtcggc cacgtcgttg gccgcgtcca cgacctcctt ggactgccac
     6541 gcggtggcct ccagcgccgc ccgggcgatg tggccctgat tcgagtaggc ggtcaggccg
     6601 cagatcacgc cgcgggcgtc gtcgcgccag tgcggggcga acaggcccga gaacgcgggc
     6661 acgaagtaca ccccgccgtt gtcgtcgacc gtcttggcca gctcctcgac ggcaggcgcg
     6721 ctgtcgatga tcttgagccg gtcgcgcagc cactggatga gcgcgccggt gacggcgatc
     6781 gatccctcga gcacgtagcg ggccgggccg tcgccgatct tgtagccgac cgacgtcagc
     6841 agcttctcg
//
LOCUS       DASUPW010000017         5525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_280283, whole genome shotgun sequence.
ACCESSION   DASUPW010000017 DASUPW010000000
VERSION     DASUPW010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280283"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..922)
                     /locus_tag="VFY44_00345"
     CDS             complement(158..922)
                     /locus_tag="VFY44_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5908907.1"
                     /translation="MSILDVQDLTIRFGGVTALDGVSFDVPEGCVCGLIGPNGAGKTT
                     LFNCVSRLYEPQSGSLAFDGHDLLGVRAHQIAGLGIARTFQHLGIVQSLTVRQNVMLG
                     GHVHGRSGFLSSALGLPRERSEERELGERADRAIEQLRLTDVAAEKAAGLSYGVLKRV
                     ELARALCTRPRLLMLDEPAGGLSQDEVRELGDLIGTLRDEHDLTILLVEHHMGLVMRV
                     SDHVVVLNFGQVIATGPPDSVQNDDAVVEAYLGSGE"
     gene            complement(970..2820)
                     /locus_tag="VFY44_00350"
     CDS             complement(970..2820)
                     /locus_tag="VFY44_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX5908908.1"
                     /translation="MAVVTASDLGKDMAGKPLLRGVSFKLERRDRMTLSGRNGSGKTT
                     LLRILAGQTGHDGGKLSLQKGAKVSLHDQRPPRERDLTLRDYVLSGAEEAVALEAELA
                     RLEQAMADGDGSDQLLAAYSKTQERFDLRGGYRWRDEALATMHGLGFRDGDLDRPLST
                     FSGGELTRGSLARALTGEPDLLLLDEPTNHLDIASLEWLEDHLNALDAAIVLVAHDRW
                     FLEAVGTSVLELEAGRSKFFPGTWTAWRKEQAMREISLGKAIEKQEAEIERLERFVTR
                     FKAKASKAKQAQSRVKRLDKIEKIERDPKDNRSLSFSFKEPERSGRTVFEIKGGRIEV
                     PGRVLIEKADMHVERAEHVTLVGGNGSGKTTLIQALTGRAELPEGKLSTGHNVHLGYV
                     SQHAEGLSEDGSLLDATTRATGLTPGKARALLGMFLFSGDEAEKPVAGLSGGERQRFS
                     LAVLVNSGANMLVLDEPTNHLDLESREALEDALRGFPGSLLLVSHDRALLDAVGNRTV
                     AIEDGELRSYEGGWADYLEARAAREEAAKPAPPPKKPKAKKQQPKAKPVDYSRLERQI
                     EEAEAALAEVEHELADPSAWSDPRTSAKSEKRHADAKRRVDELYAQLEAV"
     gene            complement(2842..3213)
                     /locus_tag="VFY44_00355"
     CDS             complement(2842..3213)
                     /locus_tag="VFY44_00355"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5908909.1"
                     /translation="MLRAFPVVYAHEVQPMVEFYEELGFSISLRIPEQGEAGYVTLVR
                     DEAEMAIVDAEWPARQTSLEMGTDPRFEMFVYVDDLEETVEALRSRGAPVLQEPTDQP
                     WGERTAYVTDPEGNPVSLALA"
     gene            complement(3213..4118)
                     /locus_tag="VFY44_00360"
     CDS             complement(3213..4118)
                     /locus_tag="VFY44_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5908910.1"
                     /translation="MAKIPHTDLDVHPLCLGGNVFGWTADRDQSFAVLDAYAAAGGNF
                     IDTADLYAGGESEKIVGAWMAERGNRDDVVVATKMGMEDGSKNLRADTIRAQAEASLA
                     ALGTDRIDLYYAHQDDEDTPVAETLGGFDALVREGKVRHIAASNFSAERLAEAEETSR
                     REGLARYVALQPHYNLMVRDEFEGAVQDICLDNDLAVFPYFALASGFLTGKFRSADAD
                     SKRGNRYEDDPIGPAVLEALDEVAGAHGVPPAAVALAWLAAQDTVLSPIASARTTEQL
                     DDLLAFVGLELSGDELDRLTAASAR"
     gene            4156..4362
                     /locus_tag="VFY44_00365"
     CDS             4156..4362
                     /locus_tag="VFY44_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908911.1"
                     /translation="MEGEEDIETKALRRQQAEIAEANREHAEEATQGEEADAAERRAE
                     KAEYLKQKLEERAAAEREADAEGA"
     gene            complement(4359..5159)
                     /locus_tag="VFY44_00370"
     CDS             complement(4359..5159)
                     /locus_tag="VFY44_00370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5908912.1"
                     /translation="MSKRVIITGAAGGLGKQATEELRRQGATVVGLDLKGNGDDILEC
                     DVRDQESVDAAVAAAIEKLGGGVDVLINNAGVGDPSSAGAAPDEAALALIDINLMGAW
                     RVTSAALDALLASRGRVVNIASGLAHVTSPFGTAYTMSKRGLVGYSDSLRLEYAGRLT
                     VSTVYPGYIRTPIHDASKEKGMGLEGAVPAEDIADAGRAFARAALSEKPVRDLATTRS
                     GTVNYAVVRLMPRGLLDKISLLRMRQLARKGHFDGGEMSREFGAALKG"
     gene            complement(5156..>5525)
                     /locus_tag="VFY44_00375"
     CDS             complement(5156..>5525)
                     /locus_tag="VFY44_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908913.1"
                     /translation="PNLFMLLGPNTGLGHSSMIYMIESQIEHVIDALAEMRRHDADTV
                     EVREDAAREFNAEIDKRHEGAVWNTGCASWYLDDTGRNATLWPDWTFRFRQRTAKFEP
                     GKYELTTHTPAPREREEVAA"
BASE COUNT          729 a         2005 c         1948 g          843 t
ORIGIN      
        1 gtgacgctcg taacgctccc gtgacgggcc acgaacccgc cttcgcgctg agtcacgact
       61 tccgtctgct cagccgacgg aactcgggtc gcaagaacga ccccgatcgc cgagggagtc
      121 gagtgttgcg gcggcgtcac agcccgcgcc agggccctca ctctcccgac cccagatacg
      181 cctcgaccac ggcgtcgtcg ttctgcacgc tgtcgggtgg ccccgtggcg atgacctggc
      241 cgaagttcag gaccaccacg tggtcggaca cgcgcatgac caggcccatg tggtgctcga
      301 ccagcaggat ggtcaggtcg tgctcgtcgc gcagcgtgcc gatcaggtcg cccagctcgc
      361 gcacctcgtc ctggctcagg ccgccggcgg gctcgtccag catcagcagc cggggccggg
      421 tgcacagcgc acgggccagc tcgacgcgct tgaggacgcc gtaggacagg ccggcggcct
      481 tctcggcggc gacgtcggtc aggcgcagct gctcgatggc acggtcggcg cgctcgccca
      541 gctcgcgctc ctcgctgcgc tcgcgcggga ggcccagggc ggaggacagg aatcccgagc
      601 gcccgtgcac gtggccgccg agcatcacgt tctggcgcac ggtcagcgac tgcacgatgc
      661 ccaggtgctg gaacgtgcgg gcgatgccca ggccggcgat ctggtgcgcc cgcacgccga
      721 ggaggtcgtg gccgtcgaag gccagcgagc cggactgggg ctcgtacagg cggctgacgc
      781 agttgaacag cgtcgtcttg cccgccccgt tggggccgat caggccacag acgcagcctt
      841 cgggcacgtc gaacgagacg ccgtcgagag ccgtcacgcc cccgaaacgg atcgtcagat
      901 cctggacatc cagaatggac atccctccct cctctcctcg gaggcgcccc taccctcggg
      961 tcttcggggt caaacggctt cgagctgtgc gtacagctcg tcgacgcgtc gcttggcgtc
     1021 ggcgtggcgc ttctcggact tggccgaggt gcgcgggtcg ctccaggccg aggggtcggc
     1081 gagctcgtgc tcgacctcgg ccagcgcggc ctcggcctcc tcgatctggc gctcgagcct
     1141 ggagtagtcc accggcttgg ccttgggctg ctgcttcttg gccttcggct tcttgggcgg
     1201 tggcgccggc ttggccgcct cttcgcgtgc cgcacgggcc tcgaggtagt cggcccaccc
     1261 accctcgtag ctgcgcagct cgccgtcctc gatcgccacc gtgcggttgc ccacggcgtc
     1321 cagcagcgcg cggtcgtggc tcaccagcag cagcgagccc gggaacccgc gcagcgcgtc
     1381 ctccagcgcc tcacggctct ccaggtccag gtggttggtg ggctcgtcca gcaccagcat
     1441 gttggcgccg ctgttcacca gcacggccag cgagaagcgc tggcgctcgc cgccggacag
     1501 cccggccacc ggcttctcgg cctcgtcgcc gctgaacagg aacatgccca gcagcgcacg
     1561 cgccttgccc ggcgtcaggc cggtggcgcg cgtggtggcg tccagcagcg agccgtcctc
     1621 ggacagcccc tcggcgtgct ggctgacgta gcccaggtgc acgttgtggc cggtgctgag
     1681 cttgccctcg ggcagctcgg cgcggccggt cagcgcctgg atcagggtgg tcttgcccga
     1741 gccgttgccg cccacgagcg tcacgtgctc ggcgcgctcc acgtgcatgt cggccttctc
     1801 gatcagcacg cggccgggca cctcgatccg cccgcccttg atctcgaaca ccgtacggcc
     1861 cgagcgctcg ggctccttga agctgaagct cagcgagcgg ttgtccttcg ggtcgcgctc
     1921 gatcttctcg atcttgtcca ggcgcttgac ccgcgactgg gcctgcttgg ccttgctggc
     1981 cttggccttg aagcgcgtga cgaaccgctc gagccgctcg atctcggcct cctgcttctc
     2041 gatcgccttg cccagcgaga tctcgcgcat ggcctgctcc ttgcgccacg cggtccacgt
     2101 ccccgggaag aacttggagc ggcccgcctc cagctccagc accgacgtgc ccacggcctc
     2161 caggaaccag cggtcgtggg ccaccagcac gatggcggcg tccagcgcgt tcaggtggtc
     2221 ctccagccac tccagcgagg cgatgtccag gtggttggtg ggctcgtcca gcagcagcag
     2281 gtcgggctcg ccggtcagcg cgcgggccag cgagccgcgc gtcagctcgc cgcccgagaa
     2341 cgtggacagc ggccggtcca ggtcgccgtc acggaagccc aggccgtgca tggtggccag
     2401 cgcctcgtcg cgccagcggt agccgccgcg caggtcgaag cgctcctggg tcttcgagta
     2461 ggcggccagg agctggtcgc tgccatcgcc atcggccatc gcctgctcca ggcgggccag
     2521 ctcggcctcc agcgccaccg cctcctcggc ccccgacagc acgtagtcgc gcagcgtcag
     2581 gtcgcgctcg cgcggggggc gctggtcgtg gagcgagacc ttggcgccct tctgcaggct
     2641 gagcttgccg ccgtcgtggc ccgtctggcc ggccaggatc cgcagcagcg tggtcttgcc
     2701 cgacccgttg cgcccggaca gggtcatgcg gtcgcggcgc tcgagcttga acgagacgcc
     2761 ccggagcagg ggcttgcccg ccatgtcctt gcccagatcg gatgcagtga ccaccgccat
     2821 gacgggtcga tggtaggcgg gctaggccag cgccagcgag acggggttgc cctcggggtc
     2881 ggtcacgtac gcggtgcgct cgccccaggg ctggtcggtg ggctcctgga gcaccggggc
     2941 gccgcgcgag cgcagggcct cgaccgtctc ctccaggtcg tccacgtaga cgaacatctc
     3001 gaagcgcggg tcggtgccca tctccaggct ggtctgccgc gccggccact cggcgtcgac
     3061 gatggccatc tcggcctcgt cgcgcaccag ggtcacgtag ccggcctcac cctgctcggg
     3121 gatccggagg ctgatcgaga agcccagctc ctcgtagaac tccaccatcg gctgcacctc
     3181 gtgggcgtag accacgggga aggcgcgcag catcagcgcg cgctcgccgc ggtcaggcgg
     3241 tccagctcgt cgccggacag ctccaggccg acgaaggcca gcaggtcgtc cagctgctcg
     3301 gtggtgcgcg cgctggcgat gggcgagagc acggtgtcct gcgccgccag ccaggccagc
     3361 gcgaccgcgg ccggcggcac cccgtgcgcg cccgcgacct cgtccagggc ctccagcacg
     3421 gccggcccga tcgggtcgtc ctcgtagcgg ttgccgcgct tgctgtcggc gtccgcggac
     3481 cggaacttgc cggtcaggaa gccgctggcc agcgcgaagt acgggaacac cgccaggtcg
     3541 ttgtccaggc agatgtcctg gaccgcgccc tcgaactcgt cgcgcaccat caggttgtag
     3601 tgcggctgga gggccacgta gcgggccagc ccttcgcggc gcgaggtctc ctcggcctcc
     3661 gccagccgct cggcgctgaa gttggacgcg gcgatgtggc gcaccttgcc ctcccggacc
     3721 agcgcgtcga acccgccgag cgtctcggcg accggcgtgt cctcgtcgtc ctggtgggcg
     3781 tagtagaggt cgatgcggtc cgtgcccagc gccgccagcg aggcctcggc ctgggccctg
     3841 atcgtgtcgg cgcgcaggtt cttgctgccg tcctccatgc ccatcttggt ggccacgacg
     3901 acgtcgtcgc ggttgccgcg ctctgccatc caggcgccga cgatcttctc cgactcgccg
     3961 cccgcgtaca ggtccgcggt gtcgatgaag ttgccgccgg cggccgcgta ggcgtccagc
     4021 acggcgaacg actggtcgcg gtcggcggtc cagccgaaca cgttgccgcc caggcagagc
     4081 gggtgcacgt caaggtcggt gtgggggatc ttcgccatgc gcccgacgtt agaccccagc
     4141 tacattgcga cgtgagtgga gggcgaggag gacatcgaga ccaaggcgct gcgccgccag
     4201 caggccgaga tcgccgaggc caaccgcgag cacgcggagg aggccaccca gggcgaggag
     4261 gccgacgccg ccgagcgccg cgccgagaag gccgagtacc tcaagcagaa gctcgaggag
     4321 cgcgccgcag ccgaacgcga ggcggacgcc gagggcgcct agcccttcag cgccgcgccg
     4381 aactcccggc tcatctcgcc gccgtcgaag tggcccttgc gggccagctg acgcatccgc
     4441 agcagagaga tcttgtccag cagcccgcgc ggcatcagcc gcacgaccgc gtagttgacc
     4501 gtgcccgagc gggtggtggc caggtcgcgg accggcttct cggacagggc ggcgcgggcg
     4561 aacgcgcggc cggcgtcggc gatgtcctcg gccggcacgg cgccctccag gcccatgccc
     4621 ttctccttcg acgcgtcgtg gatcggcgtg cggatgtagc cgggatacac cgtgctcacc
     4681 gtgagccgcc ccgcgtactc cagccgcagc gagtccgagt acccgaccag cccgcgcttg
     4741 gacatcgtgt aggcggtgcc gaagggcgac gtcacgtggg ccagcccgga ggcgatgttg
     4801 accacgcggc cgcgtgaggc cagcagcgcg tccagcgccg ccgacgtgac gcgccaggcg
     4861 cccatcaggt tgatgtcgat cagcgccagc gcggcctcgt cgggagcggc gcccgcgctg
     4921 gaggggtcgc cgacgccggc gttgttgatc agcacgtcca cgccgccgcc cagcttctcg
     4981 atggcggcgg ccaccgcggc gtccaccgac tcctggtcgc gcacgtcgca ctccaggatg
     5041 tcgtcgccgt tgcccttgag gtcgaggccg acgacggtgg cgccctggcg gcgcagctcc
     5101 tcggtggcct gcttgcccag cccgccggcc gcgccggtga tgatcacgcg cttgctcatg
     5161 cggccacctc ctcgcgctcg cgcggagcgg gcgtgtgggt ggtcagctcg tacttgccgg
     5221 gctcgaactt ggcggtgcgc tggcggaagc ggaacgtcca gtcgggccac agggtggcgt
     5281 tgcggccggt gtcgtccagg taccacgacg cgcagccggt gttccacacc gcgccctcgt
     5341 ggcgcttgtc gatctcggcg ttgaactcgc gcgcggcgtc ctcgcgcacc tcgaccgtgt
     5401 cggcgtcgtg gcggcgcatc tcggccagcg cgtcgatcac gtgctcgatc tgcgactcga
     5461 tcatgtagat catcgagctg tggcccagcc cggtgttggg gccgagcagc atgaacaggt
     5521 tgggg
//
LOCUS       DASUPW010000018         3203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1804481, whole genome shotgun sequence.
ACCESSION   DASUPW010000018 DASUPW010000000
VERSION     DASUPW010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804481"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..1825
                     /locus_tag="VFY44_00380"
     CDS             59..1825
                     /locus_tag="VFY44_00380"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX5908914.1"
                     /translation="MPSLIAPLRLALAQIDSRVGDLEGNTGKVAEQIAAARDAGAELV
                     LFPELAVTGYPPEDLLLKEHFLQAATEMVHDLAREAEGIVALVGFPERTDDVYNALAV
                     LAEGELQAVYRKSVLPNYGVFDEQRYFQAGASPATIDVNGTSIGLSICEDIWMPGPPA
                     STESLAGARVIVNLSASPYHARKGSEREQMLVQRARDYQSAILFCNLVGGQDELVFDG
                     HSLALDQKGRVVARAPQFEESLTVCTIDPAAVTGARLKDSRRRADVRSARRAGELSTV
                     TPLAALEVPGEPTEVGGPVAEPLGDEAEIYGALRTGLRDYADKNGFERVVFGLSGGID
                     SALVAMLAVDALGPDRVTSVVMPSTYSSDATQSDARTIAANLGTEIVEIAIEPAMRVY
                     EELLADAFAGAEPDITEENLQARIRGNLVMALSNKYGWLVLTTGNKSELSVGYATLYG
                     DMAGGFAVLKDVFKGWVYRLVEWRNQQEGRELVPSQVITRPPSAELRHDQRDDQSLPP
                     YDVLDGILAGYIEEDLDAAQLVARGYAEGDVDKTIALVDRAEYKRRQAPPGIRISTRA
                     FGRDRRLPITSRYRTRVTPARA"
     gene            complement(1806..2216)
                     /locus_tag="VFY44_00385"
     CDS             complement(1806..2216)
                     /locus_tag="VFY44_00385"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX5908915.1"
                     /translation="MTDTPDTPLSTANSMEGTLGFEILEYGAERGRARFAVDERVLQP
                     FGIVHGGAYAALAESLASWCTYEAVRGDGNVAMGQSNFTSFMRPVSEGTVTAEAHCTH
                     RGRTSWVWEVDFTDDRGKRCASTRVTMAVRPAPE"
     gene            2241..>3203
                     /locus_tag="VFY44_00390"
     CDS             2241..>3203
                     /locus_tag="VFY44_00390"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX5908916.1"
                     /translation="MATFDQLSAEQRAIIELVLKQGQEYEQLGTMLGLPTSRVRELAR
                     EALVRLSPVSAAAVDDDWRGQLADYLLNQQSGPEATATKGHLRRSEAARGWARSVLDS
                     LDQFYDPKNLPTIPEGEPAAAPRRERKPKPERKPREKREKKEGGGLGDRLAAMDTFAR
                     RRLLGGLGLAVVLLFAVLVWPVGALTGGGDDEKPPAKKASNADARILGQLVLRPVGNS
                     DSKDAGVAVVAERGGQRQLIVQAQLTPSKNREAYEVWLYNSPTDAKSLGAQVTDQNGT
                     FQGAGPLPANFERYKFIDVSVEKVDQNRKHSGNSVLRGQLDQLQA"
BASE COUNT          480 a         1169 c         1138 g          416 t
ORIGIN      
        1 cgccctgcgc gagttcgccg aggcgtggct gggtcagggc ctgggaagat agggagccat
       61 gccgtcgctc atcgctccgc tgcgcctcgc cctcgcgcag atcgacagcc gggtcggcga
      121 cctcgagggc aacacgggca aggtcgccga gcagatcgcg gctgcgcgcg acgccggcgc
      181 cgagctcgtg ctgttccccg agctggcggt gaccggctac ccgcccgagg acctgctgct
      241 gaaggagcac ttcctgcagg cggccaccga gatggtgcac gacctggcgc gcgaggcgga
      301 ggggatcgtc gcgctcgtcg gcttccccga gcgcaccgac gacgtctaca acgcgctggc
      361 cgtgctggcg gagggcgagc tgcaggccgt ctatcgcaag tcggtgctcc ccaactacgg
      421 ggtcttcgac gagcagcgct acttccaggc cggcgcctcc cccgccacca tcgacgtgaa
      481 cggcacctcg atcgggctgt ccatctgcga ggacatctgg atgcccggcc cgcccgccag
      541 caccgagtcg ctggccggcg cgcgcgtgat cgtgaacctg tccgcgtcgc cctaccacgc
      601 ccgcaagggc tctgagcgcg agcagatgct ggtgcagagg gcgcgcgact accagtcggc
      661 gatcctgttc tgcaacctgg tgggcggcca ggacgagctg gtcttcgacg gccacagcct
      721 cgcgctggac cagaagggtc gcgtggtggc gcgggcgccc cagttcgagg agtcattgac
      781 ggtgtgcacc atcgaccccg cggccgtgac cggggcccgg ctcaaggact cgcgccgccg
      841 ggccgacgtg cgcagcgcgc gccgtgccgg ggagctgtcc accgtgaccc cgctggcggc
      901 gctggaggtg ccgggcgagc cgacggaggt cggcgggccg gtggccgagc cgctgggcga
      961 cgaggccgag atctacggcg cgctgcgcac cggcctgcgc gactacgccg acaagaacgg
     1021 cttcgagcgg gtggtgttcg gcctctccgg cggcatcgac tccgcgctgg tggccatgct
     1081 ggccgtggac gccctggggc ccgaccgcgt gacctccgtg gtcatgccct ccacctactc
     1141 cagcgacgcc acccagtcgg acgcgcgcac gatcgcggcc aacctgggca ccgagatcgt
     1201 ggagatcgcg atcgagcccg cgatgcgggt gtacgaggag ctgctggccg acgccttcgc
     1261 cggcgccgag ccggacatca ccgaggagaa cctgcaggcc cggatccgcg gcaacctggt
     1321 catggcgctg tccaacaagt acgggtggct ggtgctgacc acgggcaaca agtccgagct
     1381 ctcggtgggc tacgccacgc tctacggcga catggccggc ggcttcgccg tgctcaagga
     1441 cgtgttcaag ggctgggtct accggctggt cgagtggcgc aaccagcagg aggggcgcga
     1501 gctggtgccg tcccaggtga tcacccggcc gccgtcggcc gagctgcgcc acgaccagcg
     1561 cgacgaccag tcgctgccgc cctacgacgt gctcgacggg atcctcgcgg gctacatcga
     1621 ggaggacctg gacgccgccc agctggtggc gcgcggctac gccgaagggg acgtcgacaa
     1681 gaccatcgcc ctggtcgacc gggccgagta caagcgccgc caggccccgc ccggcatccg
     1741 catctccacc cgcgcgttcg gccgcgaccg gcgcctgccg atcaccagcc gctaccgcac
     1801 cagggtcact ccggcgcggg cctgaccgcc atcgtcacgc gcgtcgacgc gcaccgcttg
     1861 ccccggtcgt cggtgaagtc gacctcccac acccagctgg tgcggccgcg gtgggtgcag
     1921 tgcgcctccg cggtcaccgt gccctcggac acggggcgca tgaagctcgt gaagttggac
     1981 tggcccatgg ccacgttgcc gtcgccgcgc accgcctcgt aggtgcacca ggaggccagc
     2041 gactcggcca gcgcggcgta ggcgccaccg tgcacgatcc cgaagggctg gagcacccgc
     2101 tcgtcgaccg cgaaccgcgc ccggccccgc tcggcgccgt actcgaggat ctcgaacccc
     2161 agcgttccct ccatgctgtt ggcggtcgag agcggggtgt ctggcgtgtc ggtcacgcgg
     2221 gcaagataca ttgcgccgtc atggccacct tcgaccagct ctctgccgag cagagggcga
     2281 taatcgagct cgtcctcaag cagggacagg agtacgaaca gctcggcacg atgctcgggc
     2341 tcccgaccag ccgtgtgcgc gagctggccc gtgaggcgct ggtacgcctg tcccccgtct
     2401 cggcggccgc cgtcgacgac gactggcgcg gccagctggc cgactacctg ctgaaccagc
     2461 agagcggccc ggaggcgacg gccaccaagg gccacctgcg ccgctccgag gcggcacgcg
     2521 gctgggcccg gtcggtgctg gactccctgg accagttcta cgacccgaag aacctgccga
     2581 ccatccccga gggcgagccc gccgcggccc cgcggcgcga gcgcaagccc aagcccgagc
     2641 gcaagcccag ggagaagcgc gagaagaagg aaggcggcgg cctcggcgac cgcctggccg
     2701 ccatggacac cttcgcccgc cgccgcctgc tgggcggcct cgggctggcc gtggtgctgc
     2761 tgttcgccgt cctcgtctgg cccgtgggcg ccctgaccgg cggtggcgac gacgagaagc
     2821 cgccggccaa gaaggcctcg aacgccgacg cgcgcatcct cggccagctg gtcctgcgcc
     2881 cggtgggcaa ctccgactcc aaggacgccg gcgtggccgt ggtggccgag cgcggcggcc
     2941 agcgccagct gatcgtgcag gcccagctga cgccgagcaa gaaccgcgag gcgtacgagg
     3001 tgtggctcta caactcgccg acggacgcca agtcgctggg cgcccaggtg accgaccaga
     3061 acggcacctt ccagggcgcc ggcccgctgc cggccaactt cgagcgctac aagttcatcg
     3121 acgtctcggt cgagaaggtc gaccagaacc gcaagcactc gggcaactcg gtgctgcgcg
     3181 ggcagctcga ccagctgcag gcg
//
LOCUS       DASUPW010000019         2785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_419353, whole genome shotgun sequence.
ACCESSION   DASUPW010000019 DASUPW010000000
VERSION     DASUPW010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419353"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..974
                     /locus_tag="VFY44_00395"
     CDS             141..974
                     /locus_tag="VFY44_00395"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX5908917.1"
                     /translation="MIDFVTGPLDSGIDRRALAEVVLLGLLCGPLSFWVRSYRLAYPS
                     ESLAHGLLPGLVVAALAGAPLILGAAGGVAVAAVAVAVAARDHRIGSDTATAVVVSGA
                     FGLGVLLALRPDAPARLEELLFGDPLGVSGTDLAAAGILAVAGGLALAALHRPLAAVA
                     FDPGGGPALGIRPARVRLALLLVLATAVAVAVQGLGSLLVLAVLVAPAVAVGDSVRSP
                     GRAMAAGAAVAVGAGVVGLYSSYHLGGAAGASVALALCAAAGAGALLRRLAAPARPVG
                     A"
     gene            complement(934..1692)
                     /locus_tag="VFY44_00400"
     CDS             complement(934..1692)
                     /locus_tag="VFY44_00400"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEX5908918.1"
                     /translation="MQPELSLGPVTLQTFGICFALAFLASGAVVAKRLKELDKPVDWS
                     YEIVFAAAIGGLVGSRVDYLAQHWDDVSGDLLGNLFSGSGLVWFGGLVGGALGVILWA
                     RWRGFLEWRMFDACAPALALGYAVGRIGCQLSGDGDYGKPTDSFLGMSYPDGTVPTTE
                     DVWPTPVFETIAMGLVALLLWRLRDRYRPGMLFGLYLLLAGTERLLIEFIRRNDAVVA
                     GLTQPQLIGVAMIVGGAILVYVKRQPAAPAPQAA"
     gene            complement(1797..>2785)
                     /locus_tag="VFY44_00405"
     CDS             complement(1797..>2785)
                     /locus_tag="VFY44_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX5908919.1"
                     /translation="SGLQGQIRGTQRRLGVVQDDLDQARNELLTVRDKRERARDRLVR
                     ARSELKQARGGLEDRLVELYKADQPDALTVILEADGFNDLLERTDFLERISDQDGTVI
                     RKVRVLKARAEKSERQLAGLERKRQLAAETILRRRDDIAQTRDRLATAQGDLRSARNG
                     RRTILASVRQDRRHAQEDLAAMERQQSKIRSQLAGGPSVDAGPIKKGSGGLVYPTNGT
                     FTSPFGSRWGRLHAGIDIAAPVGTPIRAAKAGRVAIAGPTGGYGNYTCIQHGGSMSTC
                     YGHQSRIGVSVGQSVGQGQVIGAVGNTGNSTGPHLHFEVRIGGNPVDPMGYL"
BASE COUNT          320 a         1000 c         1080 g          385 t
ORIGIN      
        1 gtgctcatcg cccgcgcgct ggtccaggac gcgcgcgtgc tgttgaccac ctacggccgc
       61 gagctgatcg tgatccccgg cggggacggg cgcgacggcg tccgcgccgt ggccgtccag
      121 caccacgacc acccgcactg atgatcgact tcgtgaccgg ccccctggac tcgggcatcg
      181 accggcgcgc gctggccgag gtcgtgctgc tgggcctgct gtgcgggccg ctcagcttct
      241 gggtccgcag ctaccgcctg gcctacccat cggagtcgct ggcccacggg ctgctccccg
      301 ggctggtggt ggccgccctg gcgggcgcgc cgctgatcct gggtgccgcc ggcggggtcg
      361 cggtggccgc ggtggccgtg gcggtggccg cccgcgacca ccggatcggc tcggacacgg
      421 cgaccgccgt cgtggtcagc ggcgcgttcg ggctgggggt gctgctggcg ctgcgccccg
      481 acgccccggc gcggctggag gagctgctgt tcggcgaccc cctcggcgtg tccggcaccg
      541 acctggcggc cgcgggcatc ctggcggtgg cgggcgggct ggcgctggcc gcgctccacc
      601 ggccgctggc cgccgtggcc ttcgacccgg gcgggggccc ggcgctggga atccgtcccg
      661 cacgcgtgcg cctggccctc ctccttgtgc tggccaccgc ggtcgcggtg gcggtgcagg
      721 ggctgggcag cctgctggtg ctggccgtgc tggtggcccc ggcggttgcg gtgggcgact
      781 cggtgcgaag cccgggccgg gcgatggccg cgggagccgc cgtggccgtc ggggccgggg
      841 tcgtcggcct ctactcctcg taccacctcg gcggggcggc gggcgcgtcg gtggcgctgg
      901 ccctgtgcgc ggccgcgggc gccggggcgc tgctcaggcg gcttgcggcg ccggcgcggc
      961 cggttggcgc ttgacgtaca ccaggatcgc gccgccgacg atcatcgcca cgccgatcag
     1021 ctgcggctgg gtcaggcccg cgaccaccgc gtcgttgcgg cggatgaact cgatcagcag
     1081 ccgctcggtg ccggcgagca acaggtagag gccgaacagc atgccgggcc ggtagcggtc
     1141 gcgcagccgc cacagcagca gggccaccag gcccatcgcg atcgtctcga acacgggcgt
     1201 gggccacacg tcctcggtgg tcggcaccgt cccgtcggga tacgacatcc ccaggaagct
     1261 gtcggtcggc ttgccgtagt cgccgtcgcc ggagagctgg cagccgatcc gcccgaccgc
     1321 gtagcccagg gccagcgccg gagcgcacgc gtcgaacatg cgccactcca ggaagccgcg
     1381 ccagcgcgcc cagaggatca cgccgagcgc gccgccaacc aacccgccga accagaccag
     1441 gcccgagccg gagaacaggt tgcccagcag gtcgccggac acgtcgtccc agtgctgggc
     1501 caggtagtcc acccgcgagc ccaccagtcc gccgatggcg gcggcgaaga cgatctcgta
     1561 cgaccagtcc accggcttgt cgagctcctt gagccgcttc gccacgacgg cgccggaggc
     1621 caggaacgcg agcgcgaagc agatcccgaa ggtctggagg gtgacggggc cgaggctcag
     1681 ttcgggctgc acgacgccgg accctagtca ggagcgccgc gaggagggcg gcgaaggcgc
     1741 ccggtgtcgg ggccaggatg gccccgtccc cgtcagagcg cggccctgga cggccgctag
     1801 aggtaaccca tcggatcgac ggggttcccg ccgatccgga cctcgaagtg caggtgcggc
     1861 cccgtggagt tgccggtgtt gcccacggcg ccgatgacct ggccctggcc gaccgactgg
     1921 cccaccgaca cgccgatgcg cgactggtgg ccgtaacagg tggacatgct gccgccgtgc
     1981 tggatgcagg tgtagttgcc gtagccgccg gtcgggccgg cgatggccac gcggccggcc
     2041 ttggcggcgc ggatgggcgt gcccaccggc gcggcgatgt cgatgccggc gtgcaggcgt
     2101 ccccagcgcg agccgaaggg cgaggtgaag gtgccgttgg tcgggtagac gagcccgccc
     2161 gagcccttct tgatcggccc ggcatcgacg ctgggcccac cggccagctg gctgcggatc
     2221 ttggactgct ggcgctccat ggcggccagg tcctcctggg cgtggcggcg gtcctggcgg
     2281 acgctggcca ggatggtgcg gcggccgttg cgcgcgctgc gcaggtcgcc ctgggcggtg
     2341 gccaggcggt cgcgcgtctg ggcgatgtcg tcgcggcggc gaaggatggt ctcggcggcc
     2401 agctggcgct tgcgctccag gccggcgagc tggcgctcgg acttctcggc gcgcgccttg
     2461 aggacgcgca ccttgcggat caccgtgccg tcctggtcgg agatgcgctc caggaagtcg
     2521 gtgcgctcga gcaggtcgtt gaacccgtcg gcctccagga tcacggtcag cgcgtcgggc
     2581 tggtcggcct tgtacagctc gaccagccgg tcctccaggc cgccgcgggc ctgcttgagc
     2641 tccgagcgtg cgcggacgag gcgatcgcgc gcccgctcgc gcttgtcgcg caccgtcagc
     2701 agctcgttgc gggcctggtc caggtcgtcc tgcaccacgc cgaggcgcct ctgggtgccc
     2761 cggatctggc cctgcagccc ggaga
//
LOCUS       DASUPW010000020         3171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_557639, whole genome shotgun sequence.
ACCESSION   DASUPW010000020 DASUPW010000000
VERSION     DASUPW010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557639"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VFY44_00410"
     CDS             complement(<1..407)
                     /locus_tag="VFY44_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908920.1"
                     /translation="MSGRHPRRLLAPPALVLLCLFAGPLPAASAADPVGSTTQAVAGV
                     TAAVEQQVPAAGAVRQVTANVRSSEPVRQVTAQADAVTSGTAAAAAPATSAPATPAPA
                     APQPAPAPVAEQPSATRPAAAPRAHSAAHQPPRG"
     gene            complement(552..1697)
                     /locus_tag="VFY44_00415"
     CDS             complement(552..1697)
                     /locus_tag="VFY44_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5908921.1"
                     /translation="MLAALVGFLGDFDLAEEATQEAFATAAARWPADGEPANPGAWLV
                     ATARNHAIDRIRREKTLAAKTALLDRPEAVEDEMADDTAIPDERLELIFGCCHPALAP
                     DAQVALTLRGLGGLTTPQIARAFLVPEATMAQRLVRAKRKIKAAGIPFRVPPAHELPD
                     RLAAVLAVVYLIFNQGYEGHGELTDEALHLGRALATLMPDEPEVAGLLALMLLLDSRR
                     RARFTAEGDLVLLGDQDRSLWDDGQVAGGRAALERALALRGRGPYVLQAAIAAVHAER
                     PTDWAQVAALYGELARLTDSPVVALNRAVAVGEAEGLEVGLAQIDALEGLDGYHYLHA
                     ARADLLRRLGRPAEARPAYERALELVDSGAERRFLEGRLRGLGNLGS"
     gene            complement(1730..2101)
                     /locus_tag="VFY44_00420"
     CDS             complement(1730..2101)
                     /locus_tag="VFY44_00420"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX5908922.1"
                     /translation="MCSPTEEEAMKYMLLIYGNVTQEQVDAMSGEERKQLYAEWGALN
                     RTPGMTGGVLMDDPATATTVRVDGGRTLTTDGPFVETKEALGGYFFLEADDLDAAIEV
                     AATVPPVSRGGAVEVRPVVER"
     gene            2213..>3171
                     /locus_tag="VFY44_00425"
     CDS             2213..>3171
                     /locus_tag="VFY44_00425"
                     /inference="COORDINATES: protein motif:HMM:NF023218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX5908923.1"
                     /translation="MLLACLPAAAQAAPYSSHSQLYACCTDAETKEAMFREAKDSGAA
                     YIRVDLQMEDMFSRGRAPDWAGPDEVAALSRRFDLPVLAVLVGVPRENTDCLDITDWM
                     GSFRCAPVDAAEWGEQAGQVAAHLDGVIDHFQIGNEPDGDWAFFGDAADYARMLSAAY
                     DEIHAQAPGATVVLGPTMRLNSQGASWLDQVFRTPGTDAAAKFDVASMHLRGWMTQMT
                     DAMQQRRAFLRGWGRDVPMWITEHGYSADSAWQFDPAYRGGEDAQASYLATSLPLLAK
                     AGAAQVFVTLRDGGDAQYAREGIVGGTGPAYRRKPAWYAVRDAA"
BASE COUNT          396 a         1204 c         1169 g          402 t
ORIGIN      
        1 ccccgcggcg gctggtgcgc ggcgctgtgg gcgcggggcg ccgccgccgg gcgggtcgcg
       61 gacggctgct cggccaccgg ggccggcgcc ggctggggag cggccggggc cggggtggcc
      121 ggggcggagg tcgccggcgc cgccgcggcg gcggtgccgc tcgtgaccgc gtcggcctgt
      181 gccgtgacct ggcgcaccgg ctcggagctt cggacgttcg ccgtgacctg gcgcacggcg
      241 ccggcagcgg gcacctgctg ctcgaccgcg gcggtcacgc cggcgacggc ctgcgtcgtc
      301 gagccgaccg ggtcggccgc cgaggccgcc ggcagggggc cggcgaacag gcagagcagg
      361 acgagagccg gcggcgcgag cagtcgtcgt gggtggcggc cggacatgga cccgggatgt
      421 gcccggtccg ccaggtcccg gcgcgtacac gggcctaacg cagcggttcc ggctttcaaa
      481 gcggtgcagc cgctgcgcgc cggagcctcg aaccccctac gcccgtctag gcccatcggt
      541 cggccgtggg gtcacgaccc caggttgccg agcccgcgca gccgcccctc gaggaagcgc
      601 cgctcggcgc ccgagtcgac gagctcgagc gcccgctcgt acgccggccg ggcctccgcc
      661 ggccgcccca gccggcgcag caggtcggcc cgggcggcgt gcaggtagtg gtagccgtcg
      721 aggccctcga gcgcgtcgat ctgcgccagc cccacctcga gcccctcggc ctcgcccacg
      781 gccacggcgc ggttcagcgc caccacgggc gagtcggtca gccgtgccag ctcgccgtac
      841 agcgccgcga cctgggccca gtcggtgggc cgctcggcgt gcaccgcggc gatggcggcc
      901 tggagcacgt acggtccgcg tccacggagc gccagcgcgc gctccagcgc ggcacggccg
      961 ccggccacct ggccgtcgtc ccacagcgag cggtcctggt cgcccagcag caccaggtcg
     1021 ccctcggccg tgaaccgcgc gcgccggcgc gagtccagca gcagcatcag cgccagcagc
     1081 ccggcgacct cgggctcgtc gggcatcagc gtggccagcg cgcggcccag gtgcagggcc
     1141 tcgtcggtga gctcaccgtg cccctcgtag ccctggttga agatcaggta gaccaccgcc
     1201 agcaccgccg ccagccggtc gggcagctcg tgcgcgggcg gcacccggaa cgggatgccg
     1261 gcggccttga tcttgcgctt ggcgcgcacc aggcgctggg ccatcgtggc ctcgggcacc
     1321 aggaacgcgc gtgcgatctg gggcgtggtc aggccgccga ggccgcgcag cgtcagcgca
     1381 acctgggcgt ccggcgccag cgccggatgg cagcagccga agatcagctc cagccgctcg
     1441 tcggggatcg ccgtgtcgtc cgccatctcg tcctccaccg cctcgggccg gtccagcagg
     1501 gccgtcttgg ccgccagggt cttctcccgc cggatccggt cgatcgcgtg gttgcgcgcc
     1561 gtggccacca gccacgcacc ggggttcgcc ggctcgccgt ccgcgggcca gcgcgcggcc
     1621 gcggtggcga aggcctcctg ggtggcctcc tcggcgaggt cgaagtcgcc gaggaagccc
     1681 accagcgcgg ccagcacgcg gccccactgc tcgcggaaga ccgagtcgtt cagcgctcca
     1741 ccaccggccg cacctcgacc gcgccgcccc ggctcaccgg gggcaccgtg gccgccacct
     1801 cgatggcggc gtccaggtcg tcggcctcca ggaagaagta cccgcccagc gcctccttgg
     1861 tctccacgaa cggcccgtcc gtggtcagcg tccggccgcc gtccacccgc acggtggtcg
     1921 cggtggcggg gtcgtccatc aggacgccgc cggtcatgcc gggcgtccgg ttcagggcgc
     1981 cccactcggc gtagagctgc ttgcgctcct cgccgctcat ggcatcgacc tgctcctggg
     2041 tcacgtttcc gtagatcagc agcatgtact tcatcgcttc ctcctcggtg ggtgaacaca
     2101 tgatgtcgat ccccagacga acggcgacac gccagatcga caccgaaaag actcaagcgc
     2161 ggccggatcg gggcgatact cgagtatggc ccggctcgcg accctgctcc tcgtgctgct
     2221 cgcatgcctg ccggcggccg cccaggccgc cccgtactcc tcgcattcgc agctctacgc
     2281 gtgctgcacc gacgccgaga ccaaggaggc catgttccgc gaggcgaagg acagcggggc
     2341 cgcctacatc cgcgtcgacc tccagatgga ggacatgttc agccgcggca gggcgccgga
     2401 ctgggccggg cccgacgagg tggccgcgct gtcgcggcgg ttcgacctgc ccgtcctggc
     2461 cgtgctggtg ggcgtcccgc gcgagaacac cgactgcctg gacatcaccg actggatggg
     2521 cagcttccgc tgcgcgcccg tggacgcggc cgagtggggc gagcaggccg ggcaggtggc
     2581 ggcccacctg gacggcgtga tcgaccactt ccagatcggc aacgagcccg acggcgactg
     2641 ggcgtttttc ggcgacgccg ccgactacgc ccggatgctg tcggccgcct acgacgagat
     2701 ccacgcccag gctcccggcg ccacggtggt cctggggccg acgatgcgcc tgaactccca
     2761 gggtgcgagc tggcttgacc aggtgttccg caccccgggg acggacgcgg cggccaagtt
     2821 cgacgtggcg tcgatgcacc tgcgcggctg gatgacccag atgaccgacg cgatgcagca
     2881 gcggcgggcg ttcctgcgcg gctggggccg cgacgttccc atgtggatca ccgagcacgg
     2941 ctattccgcc gatagcgcat ggcagttcga ccccgcctac cgcggcggcg aggacgccca
     3001 ggcctcctac ctggccacct cgctgccgct gctggccaag gccggcgccg cccaggtgtt
     3061 cgtgacgctg cgcgacggcg gcgacgccca gtacgcccgg gaggggatcg tgggcgggac
     3121 cgggccggcc taccggcgca agcccgcgtg gtacgccgtc cgcgatgcgg c
//
LOCUS       DASUPW010000021         4319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1527518, whole genome shotgun sequence.
ACCESSION   DASUPW010000021 DASUPW010000000
VERSION     DASUPW010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1527518"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..430)
                     /locus_tag="VFY44_00430"
     CDS             complement(227..430)
                     /locus_tag="VFY44_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908924.1"
                     /translation="MKLGKLAQMAKQQIDKRGGTEALKQDAQQLSKIAKGPGSAGDKA
                     KRAADALKEPGAPGKEPRGPHRG"
     gene            complement(488..844)
                     /locus_tag="VFY44_00435"
     CDS             complement(488..844)
                     /locus_tag="VFY44_00435"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5908925.1"
                     /translation="MSPSKLDFIGIPSTDWERSRAFYVDTLGFRPDEKSQTEVWLGET
                     CFGIWEPAKQGMEFAPQKNAHPAIHVDDVAAARAELEAKGVEFAGDTFDTGVCHMALF
                     TDPDGNDLMLHHRYAD"
     gene            complement(841..1776)
                     /locus_tag="VFY44_00440"
     CDS             complement(841..1776)
                     /locus_tag="VFY44_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908926.1"
                     /translation="MFSLGRIHIVVLGLLAALLVPTAVAAKSGKDKRDRGGKAPATIP
                     LPHGFQPEGIAAGKGGDLFVGSIPTGAIWKGDAKRGTGGVLVPGREGRAAIGLKVDRR
                     AKRIVVAGGPTGKAFVYGARDGRDIAQLQLAPAGQDTFVNDVTLNRRGAFFTDSRIKQ
                     IYRVRGNSVKTIPLTGDIAYATGNNANGIESAKGGRVLITVQTNTGKLFTVDPRTGVT
                     DEIDLGGASLPNGDGLLLKGRTLYVVQNRLNKIAVVRLARNLGSGRVVREITNPAFDV
                     PTTLAQSRKGLYTVNARFGIAPSPDNRYDVVRVGR"
     gene            1844..2764
                     /locus_tag="VFY44_00445"
     CDS             1844..2764
                     /locus_tag="VFY44_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908927.1"
                     /translation="MEYGKLINQAYRIALHNRALWPFGVFAAVGGGGFNFSFDTGGSG
                     GDGGGDSLDIDPAVIAAVVIVVLLVMALSAVATALSQGALAEGVAAIDRGEQRGFRQA
                     LRAGRATFWRVVGLYLLFVLIGLGVLLALAVVAGGAVLAAFTVTDAVVLRVLAIVFAV
                     LAALVTVVALLLPLFLIHQHAQREVVLGGARPVAALRGGWRTLRANIGSSIVLLLIQQ
                     GLVLAGYVAIGLCAAIGFIPAIVVLVASGAGAAGIVVAAVTALVVVPAALAAAGAVGT
                     FGHGLWTLGYLRMTGGQAVGWPSTTDQGVA"
     gene            2773..3258
                     /locus_tag="VFY44_00450"
     CDS             2773..3258
                     /locus_tag="VFY44_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908928.1"
                     /translation="MRSRRGVVGVAVAAVGVFGAGIVSGSDAQSPFNEYGNQRNPEPD
                     ETGPTISSKGVPTALRLSRSGTFVYTLPAFRENVYGSVSFRTAGTVTVSRKRRLTLGA
                     RTFRAKKGTRVRVRIKLNRSERKVLRRYRSLRIRARVRATDAELNDTFRNFGFTLKRP
                     R"
     gene            complement(3242..>4319)
                     /locus_tag="VFY44_00455"
     CDS             complement(3242..>4319)
                     /locus_tag="VFY44_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908929.1"
                     /translation="SELAPSPHPLANAVAALASPAGGDATGVLEVLGFLSLGLVGWVA
                     FRLGRELFSAAVGAVFAAVLLTAPPLVDATLQAGLDVPFLGLVLWAMALEAQERGRAG
                     PLVVLAVAGLLRPEAWLLAAVLVAVRAPGLDRRRLAGSVALAAAAPVVWVVSDLIATG
                     DPLHSLHATRTLGGLIGAPAGTGEAVGQAPRFLRFFLADPVVWAGLAGLPLALWLRYR
                     EALLPAAVLALGVGALAVLGVFGVPLSYRYGIVPAAMVALFAAVAVVGWTAMPRGAAR
                     SRWRAAGAAGVVAVLALSAPGVMDDLGLVTARAESERARQGRLREAVERPGAPRCVRV
                     REFLRVPEVAFWGDVRPRCVTVGA"
BASE COUNT          557 a         1599 c         1566 g          597 t
ORIGIN      
        1 ggggttggca ggcgcatcgg ccgtcgccgt ccccccgccg ccgcacgcgc acgcgttcat
       61 ggcgtccgcc ggcgcggcga cgcccagggg cagcgtcagg gcgacgccga aggcggcggc
      121 acccaccttc ggggcgatgc ccggcttgtc ttccttgatg cgggacacag gcgttccatc
      181 cccctgaaca cggaaggtga aacgccgatg cggtggcggc tgaacgtcag ccgcggtgcg
      241 gcccgcgcgg ctccttgccg ggtgcgccgg gctccttgag ggcgtcggcc gcgcgcttgg
      301 ccttgtcacc ggcgctgccg ggtcccttcg cgatcttgct gagctgctgc gcgtcctgct
      361 tgagcgcctc ggtgccgccg cgcttgtcga tctgctgctt ggccatctgg gccagcttgc
      421 ctagcttcat tgggtccttc gtcgtcgggt acggccaccc ccaagcgtag ggagtggccg
      481 aagccgccta gtcggcgtag cggtggtgca gcatcaggtc gttgccgtcc gggtcggtga
      541 acagcgccat gtggcagacg ccggtgtcga aggtgtcgcc ggcgaactcc acgcccttgg
      601 cctccagctc ggcgcgggcg gccgcgacgt cgtccacgtg gatggccggg tgggcgttct
      661 tctggggggc gaactccatg ccctgcttcg ccggctccca gatgccgaag caggtctccc
      721 ccagccacac ctcggtctgg gacttctcat ccggccggaa gccgagcgtg tcgacgtaga
      781 aggcgcgcga gcgctcccag tcggtggacg ggataccgat gaagtccagc ttcgaagggc
      841 tcatcggccg actctaacca cgtcgtagcg gttgtcgggc gagggcgcga tcccgaaccg
      901 ggcgttcacc gtgtacaggc ccttgcggct ctgggccagc gtggtgggca cgtcgaacgc
      961 ggggttggtg atctcgcgca cgacgcgccc cgaccccagg ttccgggcca ggcgcacgac
     1021 cgcgatcttg ttcagtcggt tctgcaccac gtacagcgtg cggcccttga gcagcaggcc
     1081 gtcaccgttg ggcagcgacg cgccgcccag gtcgatctcg tcggtcacgc cggtgcgcgg
     1141 gtcgaccgtg aacagcttgc ccgtgttggt ctgcacggtg atcaggaccc ggccgccctt
     1201 ggccgactcg atcccgttgg cgttgttccc ggtggcgtag gcgatgtccc cggtcagcgg
     1261 gatcgtcttc acgctgttgc cccggacgcg gtagatctgc ttgatccgcg agtcggtgaa
     1321 gaaggcgcca cggcggttca gcgtcacgtc gttgacgaag gtgtcctgcc cggccggggc
     1381 gagctggagc tgggcgatgt cgcgcccgtc gcgggcgccg tagacgaacg ccttgccggt
     1441 gggcccgccg gccacgacga tgcgcttggc acgccggtcg accttcaggc cgatcgcggc
     1501 acggccctcg cgcccgggca ccagcacgcc gcccgtgccc cgcttggcgt cgcccttcca
     1561 gatcgcgccc gtggggatcg agcccacgaa caggtcgccg cccttgcccg cggcgatgcc
     1621 ctcgggctgg aacccgtggg gcaacgggat ggtggcgggc gccttgcccc cgcggtcgcg
     1681 cttgtccttg ccggacttgg cggcgacggc cgtgggcacc agcagcgctg ccagcagccc
     1741 cagaacgacg atgtggatcc tgcccaggga gaacatggcc cggagcctac ggccgcgatg
     1801 tcacgtccgg gtaacgattc ggtaagcccc ttaggctggc gcggtggaat acggcaagct
     1861 catcaaccag gcctaccgga tcgcgctgca caaccgcgcc ctgtggccgt tcggcgtgtt
     1921 cgccgcggtc ggcggtggcg ggttcaactt ctccttcgac accggtgggt ccggtggtga
     1981 cggcggcggc gactcgctgg acatcgaccc tgccgtgatc gcggcggtgg tgatcgtggt
     2041 gctcctggtc atggccctgt ccgcggtggc caccgcgctg tcccagggcg ccctggccga
     2101 gggcgtggcg gccatcgacc ggggagagca gcgcggcttc cgccaggcgc tgcgcgcggg
     2161 gcgggcgacg ttctggcgcg tcgtcggcct gtacctgctg ttcgtcctga tcgggctggg
     2221 cgtcctcctg gcgctggccg tggtggccgg aggagcggtg ctggcggcgt tcacggtgac
     2281 cgacgccgtg gtgctgcgcg tgcttgcgat cgtcttcgcc gtgctggccg cgctggtcac
     2341 cgtggtggcg ctcctgctgc cgctcttcct gatccaccag cacgcacagc gcgaggtggt
     2401 gctgggcggc gcgcggccgg ttgccgcgct gcgcggcggc tggcgcacgc tgcgggccaa
     2461 catcggctcc tcgatcgtgc tcctgctgat ccagcagggc ctggtcctgg ccggctacgt
     2521 ggccatcggc ctgtgcgcgg cgatcgggtt catccccgcc atcgtcgtcc tggtcgccag
     2581 cggcgccgga gcggccggga tcgtcgtcgc cgcggtgacg gcgctggtgg tcgtcccggc
     2641 ggcgctggcg gccgccggcg ccgtggggac cttcggccac ggcctgtgga cgctgggcta
     2701 cctgcgtatg accggcggcc aggccgtagg atggccgtcc acaaccgacc agggggtagc
     2761 gtgagcgcag ggatgaggag caggcgggga gtcgtcggtg tggccgtggc ggccgtcggg
     2821 gtcttcggag cggggatcgt cagcggttcc gacgcccagt ccccgttcaa cgagtacggc
     2881 aaccagagga accccgagcc cgacgagacc gggcccacga tctcctcgaa gggcgtgccg
     2941 acggcgctgc gcctctcgcg ctcgggaacc ttcgtgtaca ccctccccgc gttccgcgag
     3001 aacgtctacg gctcggtcag cttccgcacc gccggcacgg tcaccgtctc gcgcaagcgg
     3061 cgcctgaccc tgggcgcccg cacgttccgc gccaagaagg gcacgcgggt ccgcgtgcgg
     3121 atcaagctga accgctccga gcgcaaggtg ctgcgccgct accggtccct gcggatccgg
     3181 gcacgcgtcc gagccaccga cgccgagctg aacgacacgt tccggaactt cgggttcacg
     3241 ctcaagcgcc cacggtgacg cagcggggcc gcacgtcgcc ccagaacgcg acctccggca
     3301 cccggaggaa ctcccggacc cggacacagc gcggcgcccc cggccgctcc acggcctcgc
     3361 gcagcctgcc ctgacgggcg cgctcgctct cggcccgcgc cgtgaccagg ccgaggtcgt
     3421 ccatcacgcc cggggccgag agcgccagca ccgccaccac cccggcggcg ccggccgccc
     3481 gccagcgcga ccgggccgcc ccgcgcggca tcgccgtcca gcccaccacc gcgacggcgg
     3541 cgaacagggc caccatggcc gcgggcacga tcccatagcg gtaggagagc ggaacgccga
     3601 acacgcccag caccgccagc gcgcccaccc ccagcgccag gacggcggcg ggcagcaggg
     3661 cttcgcggta gcgcagccac agtgccagcg gaagaccggc caggcccgcc cacaccacgg
     3721 ggtcggccag gaagaagcgc aggaaccgcg gcgcctggcc caccgcctcc cccgtgccgg
     3781 ccggcgcgcc gatcaggcct ccgagcgtgc gggtggcgtg cagggagtgc agcgggtccc
     3841 cggtggcgat gaggtcggac accacccaca cgaccggcgc cgcggccgcc agcgcgaccg
     3901 acccggccag gcgccggcgg tccaggcccg gggcgcgcac cgcgaccagc accgccgcca
     3961 gcagccatgc ctccgggcgc aggaggccgg ccaccgccag caccaccagc gggcccgccc
     4021 ggccgcgctc ctgcgcctcc agggccatgg cccacagcac cagccccagg aacgggacgt
     4081 cgaggccggc ctgcagcgtc gcgtccacca gcggcggggc cgtgagcagc accgccgcga
     4141 acaccgcgcc gaccgcggcc gagaacagct cccgcccgag ccggaacgcc acccacccca
     4201 ccaggccgag gctcaggaac cccagcacct cgagcactcc ggtggcgtcc ccaccggccg
     4261 gcgacgccag ggcggcgacg gcgttggcca gcggatgtgg cgacggggcc agctcggac
//
LOCUS       DASUPW010000022         2649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1666586, whole genome shotgun sequence.
ACCESSION   DASUPW010000022 DASUPW010000000
VERSION     DASUPW010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1666586"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VFY44_00460"
     CDS             complement(<1..794)
                     /locus_tag="VFY44_00460"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5908930.1"
                     /translation="MDDLPGKIGTDEPWYHTFDLPDGRTTKGFFDLRTVAAKVPIPAS
                     LAGKRCLDAAACEGFWSFEMARRGADEVVSVDLPHTSEQDWQGAVSDDERALGTGMAN
                     QHFYRVRETLGSPKVERRDMNLYDVGPEALGTFDYVFIGNVLVHLADPARALRALASV
                     MRPGAELLSLEANSLVLSALSPRVPTGQLWDVDDQPRWWTPNRAAHRRILHAAGYEVV
                     EQGKHLFQPFGDAIPRWPDRPPRRLRDYVFWLLVRRLGSFSGWIRAR"
     gene            854..1849
                     /locus_tag="VFY44_00465"
     CDS             854..1849
                     /locus_tag="VFY44_00465"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEX5908931.1"
                     /translation="MPSLPRPRLRRGARVGADARPANSPPEPSVETVEYPGLRWHNIE
                     RPGPIDRAWLEEHFDFHALDYEDVASRNQRPKIDEYDDYLFIVLHFPVFDKQVGRLNA
                     AELDMFVGPDFLITLPSRAIPPVDYLFERCESSEEVRESLMSKGPGYLLYKIVDDSFD
                     YCFPMLRKMGNKLERIEEEIFEGRSEEVVRDISNAKQEIINFRKIIRPQRAVLRDLER
                     TKQRYLAEDLELYFDDINDASERIWDMLENYKEVVEALEDTNESVLAHRVNDILRVLT
                     SISVIVLPLTLVASLFGMNVHVPGEGSLAAFWVVIAVMVGMLLGMLGYFRKRGWL"
     gene            complement(1846..>2649)
                     /locus_tag="VFY44_00470"
     CDS             complement(1846..>2649)
                     /locus_tag="VFY44_00470"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX5908932.1"
                     /translation="ERLDRRLGTDFLRGGWAGVGIARALAPSRFHAVRTVAAGVLLGT
                     NRWKRPVRLLLSAGGRPVPFVVPDLPALKVLAEVFVLDQYEDVWPGEPRAIVDLGAHV
                     GASALFLRGRYPDARIVCVEASPALLPILRHNTAALGVEVRHAAVAARSGTVTFYESP
                     QSWTGSLARSAIADSPVSVPTVTLDALLLDDVDLVKMDIEGSEFEIVPAATRLDQPAA
                     YMGEIHAPPDDPRTAEFLAAFEGYLTVTEDYGASTLFRATRADPARRRP"
BASE COUNT          424 a          924 c          911 g          390 t
ORIGIN      
        1 cgcgcccgta tccaccccga gaacgagccc aggcggcgca ccaggagcca gaagacgtaa
       61 tcgcggagcc ttcgcggggg ccggtcgggc caacgcggga tcgcgtctcc aaagggctgg
      121 aacaggtgct tgccctgctc caccacctcg tatcccgccg cgtgcaggat gcgccggtgc
      181 gcggcccggt tgggcgtcca ccagcggggc tggtcgtcca cgtcccacag ctgcccggtc
      241 gggacgcgcg ggctcagcgc cgacagcacc agcgaattgg cctcgagcga gagcagctcc
      301 gcgcccggcc gcatgaccga ggccagcgcg cgcagcgccc gggccgggtc ggccaggtgc
      361 acgagcacat tgccgatgaa cacgtagtcg aaggtcccga gcgcctccgg gccgacgtcg
      421 tagaggttca tgtcgcgtcg ctcgaccttc ggcgagccca gggtctcgcg cacgcggtag
      481 aagtgctggt tggccatccc cgtgcccagc gcccgctcgt cgtcggagac cgctccctgc
      541 caatcctgct cgctggtgtg gggcaggtcg acgctgacca cctcgtcggc tccccggcgg
      601 gccatctcga acgaccagaa gccctcgcag gcggcggcgt ccaggcagcg ctttcccgcc
      661 agcgacgccg gaatgggcac cttcgcggcc accgtgcgca gatcgaagaa ccccttggtc
      721 gtccggccgt ccggaaggtc gaaggtgtgg taccagggct cgtcggtccc gatcttgccg
      781 ggaaggtcat ccatgcgcgg gcaatctatg ctgaaaggca tgcccgtggt acgcgacaga
      841 ccggaggccg ccgatgccga gccttccccg gcctcgtctg cgtcgcgggg cgcgcgtcgg
      901 agctgacgcc cgtcccgcca actccccgcc ggagcccagc gtcgagaccg tcgaatatcc
      961 cggcctgcgc tggcacaaca tcgagcgtcc cggcccgatc gaccgcgcct ggctggagga
     1021 gcacttcgac ttccacgccc tggactacga ggacgtggcc tcgcgcaacc agcggccgaa
     1081 gatcgacgag tacgacgact acctgttcat cgtcctgcac ttcccggtgt tcgacaagca
     1141 ggtcgggcgc ctgaacgccg ccgagctgga catgttcgtg ggcccggact tcctgatcac
     1201 gctgcccagc cgcgcgatcc cgccggtgga ctacctgttc gagcgctgcg agtcctcgga
     1261 ggaggtgcgc gagagcctga tgagcaaggg cccgggctac ctgctctaca agatcgtcga
     1321 cgactcgttc gactactgct tccccatgct ccgcaagatg ggcaacaagc tcgagcggat
     1381 cgaggaggag atcttcgagg ggcggtccga ggaggtcgtg cgcgacatct ccaacgccaa
     1441 gcaggagatc atcaacttcc gcaagatcat ccggccccag cgcgcggtgc tgcgcgacct
     1501 cgagcgcacc aagcagcgct acctggccga ggacctggag ctgtacttcg acgacatcaa
     1561 cgacgcctcc gagcgcatct gggacatgct cgagaactac aaggaggtcg tcgaggcgct
     1621 ggaggacacc aacgagtcgg tgctggcgca ccgggtcaac gacatcctgc gggtgctgac
     1681 ctcgatctcg gtgatcgtgc tgccgctgac gctggtggcc agcctcttcg gcatgaacgt
     1741 ccacgtgccc ggcgagggct cgctggccgc gttctgggtc gtgatcgcgg tcatggtggg
     1801 catgcttctg ggcatgctcg gctacttccg caagcgcggc tggctctagg gccgcctgcg
     1861 ggcgggatcc gccctggtcg cccggaacag cgtggacgca ccgtagtcct cggtgacggt
     1921 cagatagccc tcgaaggccg cgaggaactc ggcggtgcgc ggatcgtccg ggggcgcatg
     1981 gatctccccc atgtacgcgg ccggctggtc gagccgtgtg gcggccggga cgatctcgaa
     2041 ctcggacccc tcgatgtcca tcttcacgag gtcgacgtcg tcgagcagga gggcgtcgag
     2101 cgtcaccgtc ggcacgctga cggggctgtc cgcgatcgcc gacctggcca gggaaccggt
     2161 ccagctctgg gggctctcgt agaacgtcac cgtgccggac ctggcggcga cggcggcatg
     2221 cctgacctcc acgcccagcg cggcggtgtt gtgcctgagg atcggaagca gggccgggct
     2281 cgcctccacg cacacgatcc gggcatcggg gtagcggcca cgcaggaaca gggcgctggc
     2341 cccgacgtgt gctcccaggt cgacgatggc tcgcggctcg ccgggccata cgtcctcgta
     2401 ctggtccagc acgaacacct ccgcgaggac cttgagggcg ggaaggtccg gcaccacgaa
     2461 cggcacgggt cgaccgccgg ccgagagcag caggcggacg gggcgcttcc accgattggt
     2521 gccgagcagc acaccggcgg cgacggtcct cacggcgtgg aagcgggagg gtgccagtgc
     2581 ccgggctatg cccaccccgg cccacccgcc ccgcaggaaa tcggtcccga gccttcgatc
     2641 gagacgctc
//
LOCUS       DASUPW010000023         2351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_420478, whole genome shotgun sequence.
ACCESSION   DASUPW010000023 DASUPW010000000
VERSION     DASUPW010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420478"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..912)
                     /locus_tag="VFY44_00475"
     CDS             complement(142..912)
                     /locus_tag="VFY44_00475"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX5908933.1"
                     /translation="MADRATILRELHEAAEILVLANVWDAASARAVAAVDGVRALATA
                     GHSIAAAHGYEDGENIPLDLHLAAVERVVAATELPVSMDFESGYGDPGETTRRALAAG
                     AVGANLEDGMRPLGEAVAAVEAVVAARESEGTGFVLNARTDAFLVAAEPDVETVAEAA
                     ERGRAFLDAGAECVFVPRVRQVAHIEALVGELGERRLSVIASPGAPAAAELERLGVAR
                     VSMGPWSYRAALTALQDAAAQMVGGGAFPDDVRADALP"
     gene            complement(905..2041)
                     /locus_tag="VFY44_00480"
     CDS             complement(905..2041)
                     /locus_tag="VFY44_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5908934.1"
                     /translation="MSAPTTIFTEMTALAERTGAINLGQGFPDDDGPSVIVDAAVEAL
                     RAGHNQYAPLPGIPVLRQAIAEHQQRRYGLDVNPATGVQVTFGATEAIAASLLAFLED
                     GDEVIALDPSYDAYAPIARRAGARVRPIVLDPPGWRIEETAIEAALTDRTRVLVLNSP
                     HNPTGRVLERYELELLAAVCRRHDLIAVTDEVYEHLVYDGTHVPLATLPGMAERTVTT
                     SSLGKTHSLTGWKVGWASGPPDLIARVRGVKQFLTFAGGTPFQHAAAVGVAMPDEQTG
                     ELARSLEGKRDRLTAGLREVGFAPLPTAGTYFLNADAGPLGHDDARELCLTLPSAAGV
                     AAIPVSAFSTRPERVRSLVRFAFCKRDLVLDEAVDRLKGWAGHG"
     gene            complement(2053..>2351)
                     /locus_tag="VFY44_00485"
     CDS             complement(2053..>2351)
                     /locus_tag="VFY44_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5908935.1"
                     /translation="EPCLADAGVRRDTAAFVREVDPGELLDIASRLGSFERPVLLVWG
                     DADRFFKVDFARRLAKIFPDARLVEVPGGKTFLSLDHPGRLADEIASFAPVAAA"
BASE COUNT          297 a          929 c          843 g          282 t
ORIGIN      
        1 cgcggaagcc cgccagcatg ccccggccgc ggtactccag accgcgctcg gcggcgtagc
       61 cctcgagcgt ggccgccggc ccgcgccgct tcgcgtcctc gcggtcctcc ggcgcgaacg
      121 ccgcgtagcc gtgcttgccc atcagggcag cgcgtccgcg cgcacgtcgt ccgggaaggc
      181 gcccccgccg accatctgcg cagcggcgtc ctgcagcgcg gtcagcgccg cccggtagct
      241 ccacggtccc atcgagaccc gggccacacc caggcgctcc agctcggccg ccgccggcgc
      301 gcccggtgag gcgatcacgc tgagcctgcg ctcgcccaac tcgcccacga gcgcctcgat
      361 gtgggccacc tggcgcacgc gcggcacgaa cacgcactcg gcgccggcgt ccaggaacgc
      421 ccggccgcgc tcggccgcct cggccaccgt ctccacgtcc ggctccgccg cgaccaggaa
      481 ggcgtcggtg cgcgcgttca gcacgaaccc cgtgccctcg gactcgcgcg cggccaccac
      541 cgcctcgacc gcggccaccg cctcgcccag cggccgcatc ccgtcctcca ggttggcgcc
      601 cacggcgccg gccgccagcg cgcgccgggt ggtctcgccc gggtcgccgt agccggactc
      661 gaagtccatc gacacgggca gctcggtggc ggccaccacg cgttcgacgg cggccaggtg
      721 caggtccagc gggatgttct cgccgtcctc gtacccgtgg gccgcggcga tcgagtggcc
      781 ggcggtcgcc agcgcacgca ccccgtccac cgcggccacg gcccgggcgc tggccgcgtc
      841 ccacacgttg gccagcacca ggatctcggc ggcctcgtgc agctcgcgca ggatggtcgc
      901 ccggtcagcc atggccggcc cagcctttca gacggtcgac cgcctcgtcc agcaccagat
      961 cgcgcttgca gaaggcgaac cgcaccagcg agcggacgcg ctcgggccgg gtgctgaacg
     1021 ccgacacggg gatggcggcc acgcccgcgg cgctgggcag ggtcaggcac agctcgcgcg
     1081 cgtcgtcgtg ccccagcggc ccggcgtcgg cgttcaggaa gtaggtgccg gccgtgggca
     1141 gcggggcgaa gcccacctcg cgcagcccgg ccgtgagccg gtcgcgcttg ccctccagcg
     1201 agcgggccag ctcgcccgtc tgctcgtcgg gcatcgccac gcccaccgcc gcggcgtgct
     1261 ggaagggcgt gccgccggcg aaggtcagga actgcttgac cccgcgcacg cgcgcgatca
     1321 ggtccggcgg cccgctggcc cagccgacct tccaccccgt gagcgagtgc gtcttgccca
     1381 gcgacgacgt ggtcacggtg cgctcggcca tcccgggcag cgtggccagg ggcacgtgcg
     1441 tgccgtcgta caccaggtgc tcgtagacct cgtcggtcac ggcgatcagg tcgtgccggc
     1501 ggcagaccgc cgccagcagc tccagctcgt agcgctcgag cacccgcccg gtggggttgt
     1561 gcggcgagtt cagcaccagc acgcgcgtgc ggtcggtcag ggcggcctcg atggccgtct
     1621 cctcgatccg ccaccccggt gggtccagga cgatcgggcg gacgcgcgcc ccggcgcggc
     1681 gggcgatcgg ggcgtacgcg tcgtacgagg ggtccagcgc gatcacctcg tcaccgtcct
     1741 ccaggaaggc cagcagcgac gccgcgatgg cctcggtggc gccgaaggtg acctgcaccc
     1801 cggtggccgg gttgacgtcc agcccgtagc ggcgctgctg gtgctcggcg atggcctggc
     1861 gcagcaccgg gatgccgggc agcggggcgt actggttgtg cccggcgcgc agcgcctcga
     1921 cggcggcgtc cacgatcacg gacgggccgt cgtcgtccgg gaagccctgc cccaggttga
     1981 tggccccggt gcgctcggcc agcgcggtca tctccgtgaa gatggtcgtc ggggcgctca
     2041 cgcccccgca gactacgccg ccgcgacggg cgcgaacgat gcgatctcgt ccgccagccg
     2101 cccggggtgg tccagcgaca ggaaggtctt cccgcccggc acctccacca gtcgcgcgtc
     2161 cgggaagatc ttcgccagcc gcctggcgaa gtcgaccttg aagaaccggt cggcgtcgcc
     2221 ccacaccagc agcacggggc gctcgaacga gcccagccgc gaggcgatgt ccaggagctc
     2281 accgggatcg acctcgcgca cgaaggcggc ggtgtcgcgc cgcacgcccg cgtcggccag
     2341 gcacggctcg a
//
LOCUS       DASUPW010000024         3086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1805938, whole genome shotgun sequence.
ACCESSION   DASUPW010000024 DASUPW010000000
VERSION     DASUPW010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1805938"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VFY44_00490"
     CDS             <1..523
                     /locus_tag="VFY44_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5908936.1"
                     /translation="PDMDGLEVCRRLRGAGNRVPVLVLTARDAVEDRIDGLDAGADDY
                     LVKPFDVGELKARLRALLRRAEPDAQAGDGALAFAELTLDPDRHGVTVGDRFAELTRT
                     EYQLLELLMRNPRRVLPHDVIYDRVWGYDFGPASNALRVYVGYLRRKLEEAGARPLIH
                     TVRGVGYALREPS"
     gene            520..1965
                     /locus_tag="VFY44_00495"
     CDS             520..1965
                     /locus_tag="VFY44_00495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5908937.1"
                     /translation="MTLRARIAAVAALAVALAVVATAVFTYVAVRSSLRGEIDDSLTS
                     RAAALSGGGGGGGAPGPPPPAREGGAAPPPPVRNGPPGVLRLRGREFQRRLAPRAEPF
                     GGQGAYAQFISSDGDVLRPPGGGQDLPVSAKARALARDGGDPTLTDATVDGTHVRVLT
                     SPQPGGGAVQVARSLDEVDSQLNRILVALIVVGAVGVGLGGALGAVVARTALVPIGRF
                     TARTERLRQDADLTERMDVVGDDELARLARSYNATLDALESSAEAQRHLVADASHELR
                     TPIASLRANIQMLSDADRLPPAEREQMQADIVGELDELTSLVGDVVELARGSKPASRL
                     DDVRLDEVVAAVVEREARRAPGIGVEAPALPPTVVRGEPDRIARAVTNLVGNAVKWSP
                     EGGTVEVSLADDGVLSVRDHGPGFEEADLPHVFERFYRAAAARSTPGSGLGLAIVAQA
                     AEAHGGFAEAANADGGGALLRVGFGEPLPTP"
     gene            complement(1955..>3086)
                     /locus_tag="VFY44_00500"
     CDS             complement(1955..>3086)
                     /locus_tag="VFY44_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEX5908938.1"
                     /translation="MAARDPVGIKPLYYAESDGVMRFASEMHSFEPDWRPYVQVFPPG
                     HFWTPEDGFKRFAVAVPRQHAARRRGDEPSEEDYEETRDELIGSVHRQMMGDVPVGVF
                     LSGGLDSTLIAAIAARWCKERGTQLKTFAVGTEHSSDLAAARQAAEYLDTEHHERVFT
                     GEEAEASVAEVVRSIEHFDPSLVRSAIANFFLAEMTAKHVKVVLTGEGADELFAGYEY
                     LGDLDDPEELHDEIVRTIESLHNLNLQRADRVTMAHGLEARVPFLDREVIALALRQPA
                     RWKLSPPGTQEKMLLRKAFEGWLPDELLYRDKEQFGDGSGMADAISHLMEDTVTEEEF
                     EAERERVSPPFRNREEMAYFRIWDEYLDGVKPEATLNRFATA"
BASE COUNT          373 a         1130 c         1177 g          406 t
ORIGIN      
        1 accggacatg gacggcctgg aggtctgccg gcgcctgcgc ggcgccggca accgcgtgcc
       61 ggtgctggtg ctgaccgcgc gcgacgcggt cgaggaccgc atcgacggac tggacgccgg
      121 cgccgacgac tacctggtca agcccttcga cgtcggcgag ctcaaggccc ggctgcgcgc
      181 gctgctgcgc cgcgccgagc ccgacgcgca ggcgggggac ggggcgctcg cgttcgccga
      241 gctgacgctg gaccccgacc gccacggggt cacggtcggc gaccgcttcg ccgagctgac
      301 gcgcaccgag taccagctgc tggagctgct gatgcgcaac ccgcgccgtg tgctgccgca
      361 cgacgtgatc tacgaccgcg tgtggggcta cgacttcggc cccgcgtcca acgcgctgcg
      421 ggtctacgtc ggctacctgc ggcgcaagct cgaggaggcc ggagccaggc cgctgatcca
      481 cacggtccgc ggcgtcgggt acgcgctccg cgagccctct tgaccctgcg cgcgcgcatc
      541 gccgcggtgg cggccctggc ggtggcgctg gccgtggtgg ccacggcggt gttcacctac
      601 gtggccgtgc gctcgtcgct gcggggcgag atcgacgact cgctgaccag ccgggccgcc
      661 gccctgtccg ggggcggtgg cggtggtggc gctccgggcc cgccgccacc ggcccgcgag
      721 ggcggcgccg caccgccgcc gccggtgcgc aacggcccgc cgggggtgct gcgcctgcgc
      781 ggccgcgagt tccagcgccg gctggccccg cgggccgagc ccttcggggg ccagggcgcc
      841 tacgcccagt tcatcagctc cgacggcgac gtcctgcgcc cgcccggggg cggccaggac
      901 ctgcccgtct ccgcgaaggc gcgcgcgctg gcccgtgacg gcggcgaccc cacgctgacc
      961 gacgcgacgg tggacggcac ccacgtgcgc gtgctgacca gcccccagcc cgggggcggc
     1021 gcggtgcagg tggcgcggtc gctggacgag gtcgactcgc agctgaaccg catcctggtg
     1081 gccctgatcg tggtgggggc cgtgggcgtc gggctcggcg gcgcgctggg cgcggtggtc
     1141 gcgcgcaccg cgctggtgcc gatcggccgg ttcaccgccc ggaccgagcg cctgcgccag
     1201 gacgcggacc tgaccgagcg catggacgtg gtcggcgacg acgagctggc gcggctggcc
     1261 cgcagctaca acgccaccct ggacgcgctc gagagctcgg ccgaggccca gcgccacctg
     1321 gtggccgacg ccagccacga gctgcgtacg cccatcgcca gcctgcgggc gaacatccag
     1381 atgctctcgg acgccgatcg gctgccgccg gccgagcgcg agcagatgca ggccgacatc
     1441 gtcggcgagc tggacgagct gaccagcctg gtcggcgacg tcgtggagct ggcccgcggg
     1501 tcgaagccgg ccagccggct ggacgacgtg cgcctggacg aggtcgtggc cgccgtggtc
     1561 gagcgcgagg cccggcgcgc gcccggcatc ggcgtcgagg cgccggcgct gccgccgacg
     1621 gtcgtgcgcg gcgagccgga ccggatcgcc cgcgccgtca cgaacctggt gggcaacgcc
     1681 gtcaagtgga gccccgaggg cggcaccgtc gaggtcagcc tggccgacga cggcgtgctg
     1741 tcggtgcgcg accacggccc ggggttcgag gaagccgacc tgccgcacgt gttcgagcgc
     1801 ttctaccgcg ccgccgccgc ccgcagcacg cccggctccg ggctggggct ggccatcgtg
     1861 gcccaggccg ccgaggcgca cgggggcttc gccgaggccg ccaacgccga cggcggcggg
     1921 gcgctgctgc gggtcggctt cggcgagccg ctgcctacgc cgtagcgaag cggttgagcg
     1981 tggcctcggg cttcacgccg tccaggtact cgtcccagat gcggaagtag gccatctcct
     2041 cgcggttgcg gaagggcggg gacacccgct cgcgctcggc ctcgaactcc tcctcggtga
     2101 ccgtgtcctc catcaggtgg ctgatcgcgt cggccatgcc gctgccgtcg ccgaactgct
     2161 ccttgtcgcg gtagagcagc tcgtcgggca gccacccctc gaaggccttg cggagcagca
     2221 tcttctcctg ggtgccgggc ggcgagagct tccagcgcgc gggctggcgc agcgccaggg
     2281 cgatgacctc gcggtccagg aagggcacgc gcgcctccag gccgtgggcc atggtcacgc
     2341 ggtcggcgcg ctggaggttc aggttgtgca ggctctcgat ggtgcgcacg atctcgtcgt
     2401 gcagctcctc ggggtcgtcc aggtcgccca ggtactcgta gccggcgaac agctcgtcgg
     2461 cgccctcacc ggtcagcacg accttcacgt gcttggcggt catctcggcc aggaagaagt
     2521 tggcgatggc gctgcgcacc agcgacgggt cgaagtgctc gatcgagcgc acgacctcgg
     2581 ccaccgaggc ctcggcctcc tcgccggtga acacgcgctc gtggtgctcg gtgtccaggt
     2641 actcggcggc ctggcgggcg gcggccaggt cggacgagtg ctcggtgccc acggcgaacg
     2701 tcttcagctg cgtgccccgc tccttgcacc agcgcgcggc gatggcggcg atcagcgtcg
     2761 agtccaggcc gccggacagg aacaccccca cgggcacgtc gcccatcatc tggcggtgca
     2821 ccgagccgat gagctcgtcg cgggtctcct cgtagtcctc ctcggagggc tcgtcgccgc
     2881 ggcggcgtgc cgcgtgctgg cgcggcacgg ccaccgcgaa gcgcttgaag ccgtcctcgg
     2941 gcgtccagaa gtgcccgggc gggaacacct ggacgtaggg gcgccagtcg ggctcgaagc
     3001 tgtgcatctc cgaggcgaag cgcatgaccc cgtcggactc ggcgtagtag agcggcttga
     3061 tcccgaccgg gtcgcgcgcg gccatg
//
LOCUS       DASUPW010000025         3233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1251718, whole genome shotgun sequence.
ACCESSION   DASUPW010000025 DASUPW010000000
VERSION     DASUPW010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251718"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="VFY44_00505"
     CDS             <1..863
                     /locus_tag="VFY44_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX5908939.1"
                     /translation="LAGYRAIKVHDPRRAMRVLFGETSAISSPRDTLFSALCLNRRGR
                     PYKGAIKAAQGCSKPTKLPIGGLAVHPYNQNALGSVFSTSKTKASMPMAYLSRADRLL
                     ALAEKYKRVPKGRGIYITEFGFQSRPANALGLSLEGQAKALNESERLFFDDKRIKSVA
                     QFELYDVPDRPGEDIFNTGLRQQDGQAKPALAAWRLPLVVTRKSSRRVEVWGQVRPAR
                     GRVRAAVQVARTATGTFRTIKRVRTNRSGYFRFDLRRAGAARLSYRLSWGRHHSRIAK
                     PGRKIRYKRG"
     gene            complement(864..1616)
                     /locus_tag="VFY44_00510"
     CDS             complement(864..1616)
                     /locus_tag="VFY44_00510"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX5908940.1"
                     /translation="MLPLAAEVNTTVVAAFAVGFISFISPCVLPLVPGYLSTISGVSF
                     AEIQAGEGRSKVLGPAILFCLSFTVMFVALGMTATGFGSALQDHRRLLQQIAGVVLVA
                     LGLLFIATLFVNRLNREWRPESLLARASTGGPIVAGLAFAVAWLPCTGPTLGAILTAA
                     GTEDSVGQGGVLLAFYALGLAVPFILSALAFSSVSGFFRFFRDHYNAITVISGLVLIV
                     MGVLLYSNELTRLNAEALALMDDLGINFFSEL"
     gene            complement(1616..2131)
                     /locus_tag="VFY44_00515"
     CDS             complement(1616..2131)
                     /locus_tag="VFY44_00515"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HEX5908941.1"
                     /translation="MRRLLALLMAAALLALPAAATAADCKPRTTLGDVEDEVMCPVCG
                     TPLALATEAPQANRERAFIQGLVDDCLSKDQIKDRLVAEYGSEVLSTPDDSGFDLAAT
                     LVPAGLVLLGGGALAAAALQWRRSRGGWTSYGPLDDDPDETGVREPRRPGPPEPGASE
                     RLQDDLDRYGR"
     gene            complement(2128..2685)
                     /locus_tag="VFY44_00520"
     CDS             complement(2128..2685)
                     /locus_tag="VFY44_00520"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX5908942.1"
                     /translation="MQRLRSPFVIAALIGVTALLGLLVYGLSHNGPDRAIEDSLAKGQ
                     REAAPDFDLPRLDGGGRQTLADYRGKVVVLNVWGSWCDPCRTESPLLERWHKRISRDG
                     KGTLLGIDVLDNTPDAEAFIREYGLTYPQLKDPEDQLRPEYGVAGVPETIVIDPRGRV
                     AAVKRGPVDDAFMRQNVAPLLKAGS"
     gene            2920..>3233
                     /locus_tag="VFY44_00525"
     CDS             2920..>3233
                     /locus_tag="VFY44_00525"
                     /inference="COORDINATES: protein motif:HMM:NF025482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HEX5908943.1"
                     /translation="MTQQKTFKRRVRARMEKTGESYTAARRQLIGDGGPPPEPSFEPT
                     VAEERVVEATGHGWDHWFAALDEWGARGWSHTEAARWLRDEAGVPGWYSQAITTSYER
                     ARG"
BASE COUNT          491 a         1171 c         1170 g          401 t
ORIGIN      
        1 acctcgccgg ctaccgggcg atcaaggtac acgacccgcg ccgggccatg cgcgtgctct
       61 tcggcgagac ctcggccatc agctcgccgc gggacacgct gttctcggcg ctgtgcctga
      121 accgccgcgg ccggccctac aagggcgcca tcaaggccgc ccagggctgc tcgaagccca
      181 ccaagctgcc catcggcggc ctggccgtgc acccctacaa ccagaacgcg ctcggctcgg
      241 tgttctccac cagcaagacc aaggcctcca tgcccatggc ctacctcagc cgcgccgacc
      301 ggctgctggc gctggccgag aagtacaagc gcgtgcccaa ggggcggggc atctacatca
      361 ccgagttcgg cttccagtcg cggcccgcca acgccctcgg gctcagcctc gagggtcagg
      421 cgaaggcgct gaacgagtcc gagcgcctgt tcttcgacga caagcggatc aagtcggtgg
      481 cccagttcga gctctacgac gtgcccgacc ggccgggcga ggacatcttc aacaccggcc
      541 tgcgccagca ggacggccag gccaagccgg cgctggccgc ctggcgcctg ccgctggtgg
      601 tcacccgcaa gagctcgcgc cgcgtcgagg tctggggcca ggtgcgcccg gcccgcggcc
      661 gcgtccgggc cgcggtccag gtcgcgcgga ccgccacggg cacgttccgc acgatcaagc
      721 gggtgcgcac caaccgctcc ggctacttcc gcttcgacct ccgccgggcg ggcgccgcgc
      781 gcctgagcta ccgcctcagc tgggggcgcc accacagccg gatcgccaag ccgggccgca
      841 agatcaggta caagcgcggc tagctacagc tcgctgaaga agttgatgcc caggtcgtcc
      901 atcagcgcca gggcctcggc gttcaggcgg gtcagctcgt tgctgtagag caggaccccc
      961 atcacgatca gcaccaggcc ggagatcacg gtgatggcgt tgtagtggtc gcggaagaag
     1021 cggaagaagc cggacaccga cgagaacgcc agcgcgctga ggatgaacgg cacggccagg
     1081 cccagcgcgt agaaggccag cagcacgccg ccctggccca cgctgtcctc ggtgccggcg
     1141 gcggtcagga tggcgcccag cgttgggccg gtgcagggca gccaggccac ggcgaaggcc
     1201 aggccggcca cgatcgggcc gccggtggag gcgcgtgcca gcagcgactc gggccgccac
     1261 tcgcggttca ggcggttcac gaacagggtg gcgatgaaca gcaggcccag cgccaccagc
     1321 accacgccgg cgatctgctg gagcaggcgg cggtggtcct ggagcgccga cccgaagccg
     1381 gtggcggtca tgcccagggc gacgaacatg accgtgaacg agaggcagaa caggatcgcc
     1441 ggtcccagga ccttcgaccg gccctcgccg gcctggatct cggcgaacga gacgcccgag
     1501 atggtggaca ggtagcccgg caccagcggc agcacgcacg gggagatgaa cgagatgaac
     1561 ccgaccgcga acgcggccac gaccgtcgtg ttcacctcgg cggccagcgg gagcatcagc
     1621 ggccgtaccg gtccaggtcg tcctggaggc gctccgacgc ccccggctcc ggcggccccg
     1681 gccgccgcgg ctcgcggacc ccggtctcgt ccgggtcgtc gtccagcggg ccgtaggagg
     1741 tccatccgcc gcgcgagcgc cgccactgca gcgccgcggc cgccagggcg ccaccgccca
     1801 gtagcacgag cccggcgggc accagcgtgg cggccaggtc gaagccgctg tcgtcggggg
     1861 tcgacagcac ctcgctgccg tactcggcca ccaggcggtc cttgatctgg tccttgctca
     1921 ggcagtcgtc caccaggccc tggatgaagg cgcgctcgcg gttggcctgc ggggcctcgg
     1981 tggccagcgc cagcggcgtg ccgcacaccg ggcacatgac ctcgtcctcc acgtcaccga
     2041 gggtcgtgcg cggcttgcag tccgcggccg tggccgcggc cggcagggcc aggagcgccg
     2101 cggccatcag cagcgccagc aggcgcctca cgagccggcc ttcagcagcg gcgcgacgtt
     2161 ctggcgcatg aaggcgtcgt cgaccggccc gcgcttgacc gcggcgacgc gcccgcgcgg
     2221 gtcgatcacg atcgtctccg gcacaccggc caccccgtac tccggccgca gctggtcctc
     2281 ggggtccttg agctgcgggt aggtcagccc gtactcgcgg atgaacgcct ccgcgtcggg
     2341 cgtgttgtcc aggacgtcga tgcccagcag cgtgcccttg ccgtctcgtg agatgcgctt
     2401 gtgccagcgc tccaggagcg gcgactcggt gcggcacggg tcacaccatg agccccacac
     2461 gttcaggacc acgaccttgc cgcggtagtc ggccagcgtc tgccgcccgc caccgtccag
     2521 gcgcggcagg tcgaagtcgg gcgcggcctc gcgctggccc ttggccagcg agtcctcgat
     2581 ggcgcggtcg gggccgttgt ggctcaggcc gtagaccagc aggccgagga gggccgtcac
     2641 gccgatcagg gcggcgatca cgaaggggga gcgcaggcgc tgcatagggc gacccctgag
     2701 ggtattgggc ggcgggccgc gcttgacggg ttctcgcgtc ggttgtattt tccaaccagg
     2761 cgcggtcccg cgcccgacct ccatcccggt gctcacgccg ccggtcagcg gaggtagagg
     2821 tggcctcgag ccatcgggac accggtcatc gttcgcccta tccggtgggg catcgggagc
     2881 gtgggtcccg gggccagcca ccgtgatcag gagcacgaca tgacccagca gaagacgttc
     2941 aagcgccgcg tccgcgcccg gatggagaag acgggggaga gctacacggc ggcccgccgc
     3001 cagctcatcg gcgacggcgg cccgccgccg gagccgagct tcgagccgac cgtggccgag
     3061 gagcgggtgg tcgaggccac cggccacggg tgggaccact ggttcgccgc cctggacgag
     3121 tggggcgccc gcgggtggtc gcacaccgag gccgcgcgct ggctccggga cgaggcgggg
     3181 gtgccgggct ggtactcgca ggccatcacc acgagctacg agcgggcgcg cgg
//
LOCUS       DASUPW010000026         3158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_5248, whole genome shotgun sequence.
ACCESSION   DASUPW010000026 DASUPW010000000
VERSION     DASUPW010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5248"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VFY44_00530"
     CDS             <1..239
                     /locus_tag="VFY44_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908944.1"
                     /translation="SLFAGAGLSIVLLNVLFRIGLQGDRERDQELRAREHFAEHGDWP
                     EEAPKAEREWNLPINVATPESEAAERREREARPE"
     gene            263..649
                     /locus_tag="VFY44_00535"
     CDS             263..649
                     /locus_tag="VFY44_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5908945.1"
                     /translation="MTQAVELILARNLLSAVELAAFIIDTDGTVVFFNDSAGDLIGRR
                     FEEVGPLDQAQWSSEFGPFDEFGQLMPTDSLPLSIALREGLPANGRFSVRLEGGDLVP
                     VEVSALPLVTANGFQGAIVVFWRAAE"
     gene            653..1504
                     /locus_tag="VFY44_00540"
     CDS             653..1504
                     /locus_tag="VFY44_00540"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5908946.1"
                     /translation="MSLGVRYWGTRGSIPTPTPEMARYGGNTACVELTLSDGSQMILD
                     AGTGIRGLGQALEGGSAPVHVLLTHLHLDHIQGLMFFAPLFAPGRDVRIYGPPALGAP
                     LLNRLGRYISAPLAPVEIHELPAEVSFQEAAEGGWDVGPARVQAAFVNHRGPTLGYRI
                     TDGDATVAYLPDHEPALGQRLDGANAEWISGHELAKDADLLIHDAQYTDAEYGDHVGW
                     GHSGLADTIAFARLAGARRLSLFHHDPLHDDAALDALGGEAAAAAGNQFEDIRMAREQ
                     QTATLAP"
     gene            complement(1514..3019)
                     /locus_tag="VFY44_00545"
     CDS             complement(1514..3019)
                     /locus_tag="VFY44_00545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5908947.1"
                     /translation="MKTDSVWLHPDSAMPRFDRLEAEIEADVAVIGGGLVGITTALML
                     QEDGQDVVLLDAGRLGTGVSGHTTAKVSSQHGLKYDSLRSTYGLEAATTYGQANQVAL
                     EWMASRVKDRDIDCDFRRRPSFAYVPEGEPTDDIEQEAEAAAAAGLPATLDHSAPLPY
                     PIAAAVRFDDQAEFHVRKYMGALVRQFTAAGGRAFEHSRASQVDSGDPCLVRLPGAMV
                     KAARVVVATHYPFLDRSLAFARVHPQRSYAVLCRVKKAPPHGMFLSASSPTRSIRAVP
                     VDGEELLMVGGEGHKTGTGGDTRQRYDALRRFADEHWGVESVEYRWSSQDNSSIDKLP
                     LIGRVVPHDPNLLMATGFDKWGMTGGTAAAMILTDACADREHQWASLFDPRRLNLRAA
                     VPDGIKENAETGYRMVRDRLVNHGSRPIEELQPGEGDIVQYEGDKVAAYRDDDGRLVA
                     VSTRCTHLGCQVNWNAAERSWDCPCHGSRFAPDGAVLQGPAVHRLERKPLN"
BASE COUNT          422 a         1120 c         1171 g          445 t
ORIGIN      
        1 tctcgctgtt cgcgggggcc gggctctcga tcgtcctgct gaacgtcctg ttccggatcg
       61 gcctgcaggg tgaccgcgag cgcgaccagg agctgcgggc gcgcgagcac ttcgccgagc
      121 acggcgactg gcccgaggag gcgcccaagg ccgagcgcga gtggaacctg cccatcaacg
      181 tggccacccc ggagtccgag gcggccgagc ggcgcgagcg cgaggcgcgg cccgagtagc
      241 gggtcgtcag actacggccc acatgaccca ggccgtcgag ctgattctgg cccgcaacct
      301 cctctcggcg gtggagctgg ccgcgttcat catcgacacg gacgggaccg tggtgttctt
      361 caacgactcc gcgggcgacc tgatcgggcg ccggttcgag gaggtcgggc ccctggacca
      421 ggcgcagtgg agctcggagt tcgggccgtt cgacgagttc ggccagctga tgccgaccga
      481 ctcgctgccg ctgtcgatcg cgctccggga gggcctgccg gccaacggcc ggttcagcgt
      541 ccgcctggag ggaggcgatc tggtgccggt cgaggtcagc gcgctgccgc tggtgaccgc
      601 gaacgggttc cagggcgcga tcgtcgtctt ctggcgcgcc gcggagtagc cggtgtcgct
      661 gggcgtccgg tactggggca cccgcggatc gatcccgacc cccacgccgg agatggcccg
      721 gtacggcggc aacaccgcct gcgtggagct gacgctctcg gacggcagcc agatgatcct
      781 ggacgccggc accgggatcc gcgggctggg ccaggccctc gagggcgggt cggcgccggt
      841 gcacgtgctg ctgacccacc tgcacctgga ccacatccag gggctgatgt tcttcgcgcc
      901 cctgttcgct cccgggcgcg acgtgcgcat ctacggcccg cccgcgctgg gcgccccgct
      961 gctgaaccgc ctgggccggt acatctcggc gccgctggct ccagtggaga tccacgagct
     1021 gccggcggag gtctccttcc aggaggcggc cgaaggcggc tgggacgtgg ggccggcccg
     1081 tgtccaggcg gcgttcgtga accaccgcgg cccgacgctg ggctaccgga tcaccgacgg
     1141 cgacgcgacc gtggcatacc tgcccgacca cgagccggcg ctgggccagc ggctggacgg
     1201 ggcgaacgcc gaatggatct ccggccacga gctggccaag gacgccgacc tgctgatcca
     1261 cgacgcgcag tacacggacg ccgagtacgg cgaccacgtg ggctggggcc attcggggct
     1321 cgcggacacg atcgcgttcg cgcggctggc cggcgcccgg cggctgagcc tgttccacca
     1381 cgaccccctg cacgacgacg cggcgctgga tgccctcggg ggcgaggcgg ccgcggccgc
     1441 cgggaaccag ttcgaggaca tccgcatggc gcgcgagcag cagaccgcga cgctggcccc
     1501 ctgacccgcg gggtcagttc agcggcttgc gctccagccg gtgcacggcc gggccctgga
     1561 gcacggctcc gtccggggcg aagcgcgagc cgtggcaggg gcagtcccag ctgcgctcgg
     1621 cggcgttcca attgacctgg cagcccaggt gcgtgcaccg ggtcgagacg gccaccagcc
     1681 ggccgtcgtc gtcgcggtag gcggcgacct tgtcgccctc gtactgcacg atgtcgccct
     1741 cgccgggctg gagctcctcg atcgggcggc tgccgtggtt gaccaggcgg tcccgcacca
     1801 tgcggtagcc ggtctcggcg ttctccttga tgccgtccgg gacggcggcc cgcaggttca
     1861 gccggcgcgg gtcgaacagc gacgcccact ggtgctcgcg atcggcacag gcgtcggtca
     1921 ggatcatcgc cgccgcggtg cccccggtca ttccccattt gtcgaagccc gtggccatca
     1981 gcaggttcgg gtcgtgcggg accacgcgcc cgatcagagg cagcttgtcg atgctgctgt
     2041 tgtcctgtga ggaccaccgg tactccacgg actccacgcc ccagtgctcg tcggcgaacc
     2101 ggcgcagggc gtcgtagcgc tgccgggtgt cgccgcccgt gccggtcttg tggccctctc
     2161 cgccaaccat caggagctcc tcgccgtcga ccggcaccgc gcggatcgag cgcgtggggg
     2221 acgaggcgct caggaacatc ccgtgcggcg gggccttctt gacccggcac aggaccgcgt
     2281 aggagcgctg gggatgcacc cgcgcgaagg ccagcgaccg gtccaagaac gggtagtggg
     2341 tggccaccac gacccgcgcc gccttgacca tcgcgcccgg cagcctgacc aggcaggggt
     2401 cgcccgagtc cacctgcgag gcgcgcgagt gctcgaacgc ccggccgccg gcggcggtga
     2461 actgccggac cagcgcgccc atgtacttgc gcacgtggaa ctcggcctgg tcgtcgaagc
     2521 ggacggcggc cgcgatcggg tagggcagcg gcgccgagtg gtccagggtg gcgggcagcc
     2581 cggccgcggc agcggcttcg gcctcctgct cgatgtcgtc ggtgggctcg ccctccggca
     2641 cgtaggcgaa cgacggccgg cgcctgaagt cgcagtcgat gtcgcggtcc ttcacgcgcg
     2701 aggccatcca ctccagcgcc acctggttgg cctgcccgta cgtggtggcc gcctcgagcc
     2761 cgtacgtcga gcgcagcgag tcgtacttga gcccgtgctg ggaggagacc ttcgccgtgg
     2821 tgtggcccga gaccccggtc cccagcctgc cggcgtccag gagcaccacg tcctggccgt
     2881 cctcctgcag catcagggcg gtggtgatgc ccaccaggcc gccgccgatg accgccacgt
     2941 cggcttcgat ctcggcctcg aggcggtcga accggggcat ggccgagtcg gggtgcagcc
     3001 agacggagtc ggtcttcacg aggggcgctc ctcctggagc agcgcgagct cctccaggcg
     3061 ggccagggac tcggcgttgc gagcaagggt gaagaggtgg gtcgccgggt tcaggctcag
     3121 ccacgcgaac acgccgtcgg ggttctcgga gaagcgca
//
LOCUS       DASUPW010000027         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_143813, whole genome shotgun sequence.
ACCESSION   DASUPW010000027 DASUPW010000000
VERSION     DASUPW010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143813"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VFY44_00550"
     CDS             <1..542
                     /locus_tag="VFY44_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5908948.1"
                     /translation="ELELGLPGYDVVVGLSRPASDPVDLHRAGSEALMAATVAEARGQ
                     GRLAFEETGAYRLLLPAMSEDPGELQRFHQETVAPLIEYDNQYETDLVLTLESFLEAD
                     GNVAKTAEKLFTHRHTVRYRLDRVRELSGLDVSSTDGRERLSLGLKSMRVLGIVPPGG
                     PASEAGAGGGRVPRAEQDR"
     gene            553..1122
                     /locus_tag="VFY44_00555"
     CDS             553..1122
                     /locus_tag="VFY44_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908949.1"
                     /translation="MILPAPLRVRAFEGEVTVAVRRWKRPTVRAGGTLRTPSGVLAID
                     SVERVRLTDLTPAIARAAGYPSLKALTASLAPTRNPLYVVRFHPGGPDPRVALRERAE
                     LTEAERADLDRRLDGFDERSPRGPWTRQTLRTIAERPAVRAPDLAADAGVETKRYKTD
                     VRKLKELGLTESLEVGYRLSPRGRAYLGA"
     gene            complement(1307..1377)
                     /locus_tag="VFY44_00560"
     tRNA            complement(1307..1377)
                     /locus_tag="VFY44_00560"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1343..1345),aa:Gln,seq:ttg)
     gene            1417..1791
                     /locus_tag="VFY44_00565"
     CDS             1417..1791
                     /locus_tag="VFY44_00565"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5908950.1"
                     /translation="MIDHVGIEVSDYDRSKAFYEPVLATLGMTLLMEPADYIGGFGFP
                     DQGKPFFWIGTRTEPTHSAHVAFSAGSREIVDEFHKEAMAAGGTDNGGPGVRSVYHPH
                     YYGAFVLDLDGNNIEAVCHEPA"
     gene            1796..>2168
                     /locus_tag="VFY44_00570"
     CDS             1796..>2168
                     /locus_tag="VFY44_00570"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX5908951.1"
                     /translation="MSDRLTVLIMAAGKGTRMRSSLPKVLHPVCGKPMVQWVIDAART
                     AGADRIVAITRPGDGVAEGLPEDVGVAEQREGEGTGAAVLAARDAVDGGTVVVLSGDV
                     PLQSAELIGQLVATHREQGAAA"
BASE COUNT          301 a          816 c          786 g          265 t
ORIGIN      
        1 gtgagctgga gctgggcctg cccggctacg acgtcgtggt gggcctcagc cggcccgcgt
       61 ccgacccggt cgacctgcac cgcgccggct ccgaggccct gatggcggcc accgtggccg
      121 aggcgcgcgg ccaggggcgg ctggccttcg aggagacggg cgcctaccgc ctgctgctgc
      181 ccgccatgag cgaggacccc ggcgagctgc agcgcttcca ccaggagacc gtggccccgc
      241 tgatcgagta cgacaaccag tacgagaccg acctggtgct gacgctcgag agcttcctgg
      301 aggccgacgg caacgtggcc aagaccgcgg agaagctgtt cacccaccgc cacacggtgc
      361 gctaccggct cgaccgcgtg cgcgagctca gcggcctgga cgtcagctcc accgacggcc
      421 gggagcggct gagcctgggc ctgaagtcca tgcgcgtcct gggcatcgtc ccgcccggcg
      481 ggccggcctc cgaggccggt gccggcggcg gtcgcgtgcc gcgcgccgaa caggaccgct
      541 gacgcccgtc ccgtgatcct gcccgccccg ctgcgcgtcc gcgccttcga gggggaggtc
      601 accgtggccg tgcggcgctg gaagcggccg acggtcaggg ccggcgggac cctgcgcacg
      661 ccctcgggcg tgctggccat cgactcggtc gagcgcgtgc gcctgaccga cctgacgccc
      721 gcgatcgccc gcgccgccgg ctacccgtcg ctcaaggcgc tgaccgcgtc cctggcgccg
      781 acccgcaacc cgctctacgt ggtccgcttc cacccgggcg ggcccgaccc gcgggtcgcg
      841 ctgcgcgagc gcgccgagct gacggaggcc gagcgcgccg atctggaccg ccgcctcgac
      901 ggcttcgacg agcgctcgcc gcgcgggccc tggacgcgcc agacgctgcg caccatcgcc
      961 gagcgccccg cggtccgcgc gccggacctg gcggccgacg ccggcgtgga gaccaagcgc
     1021 tacaagaccg acgtgcgcaa gctcaaggag ctggggctga ccgagagcct ggaggtgggc
     1081 taccggctgt cgccgcgcgg gcgggcctac ctgggcgcct agccctcagt ccttggacgg
     1141 ccccggctcc acgccgggcg gcgtcggttg gacgatgacc caccacgcga cgaacgcggt
     1201 ggcggccgcc agcaccgaca ccaccatgac cgggccgtcc ggtggccgcc cgccgggccg
     1261 agctggagac cgcgctgcgg ctcagggacc tgctgggctg aggcgactgg ggggggagga
     1321 atcgaacctc cattgtcggg accaaaaccc gatgtcttgc cgttagacga ccccccatcg
     1381 tgtcggcccg caggatacgg cgtagcctgt cacccgatga tcgaccatgt gggcatcgag
     1441 gtctccgact acgaccgcag caaggccttc tacgaacccg tcctggccac cctcggcatg
     1501 acgctgctga tggagccggc cgactacatc ggcggcttcg gcttccccga ccagggcaag
     1561 ccgttcttct ggatcggcac caggaccgag cccacccaca gcgcccacgt ggccttctcg
     1621 gcgggctcgc gcgagatcgt cgacgagttc cacaaggagg cgatggcggc cggcggcacc
     1681 gacaacggcg gcccgggggt gcgctcggtc taccaccccc actactacgg cgccttcgtg
     1741 ctggacctgg acggcaacaa catcgaagcc gtctgccacg agccggcctg agcccatgag
     1801 cgaccggctg accgtgctga tcatggcggc gggcaagggg acccggatgc ggtcctcgct
     1861 gcccaaggtg ctgcacccgg tgtgcggcaa gccgatggtg cagtgggtga tcgacgccgc
     1921 ccgcaccgcc ggcgccgacc ggatcgtggc catcacccgc cccggcgacg gtgtcgcgga
     1981 aggcctgccg gaggacgtcg gcgtcgccga gcagcgcgag ggggagggca ccggcgccgc
     2041 cgtgctggcc gcccgcgacg ccgtggacgg cggcaccgtg gtggtgctct cgggcgatgt
     2101 gccgctgcag tcggccgagc tgatcggcca gctcgtggcc acccaccgcg agcaaggcgc
     2161 ggcggcca
//
LOCUS       DASUPW010000028         4425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_5515, whole genome shotgun sequence.
ACCESSION   DASUPW010000028 DASUPW010000000
VERSION     DASUPW010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5515"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /gene="rfbD"
                     /locus_tag="VFY44_00575"
     CDS             complement(<1..737)
                     /gene="rfbD"
                     /locus_tag="VFY44_00575"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEX5908952.1"
                     /translation="MKLLVCGAGGMLGQDVVRAARFTNHEVAALDRDALDVSDERAVR
                     RAMERERPAAVVNCAAYTAVDAAEDDADAAMRLNAGAAGIVAAGAAEVGASVVYPSTD
                     YVFDGTGTRPYVESDEPSPESVYGRTKLAGEVDTAAANPRHFLVRTSWLFGTGGRNFV
                     ETMLEIAERDKQVVVVRDQVGSPTWTGHLAAGIVRLLDTDAYGLHHMSAQGECSWYDF
                     AMAIFEDAGVECRVLSTTTAEFARPAPR"
     gene            807..2309
                     /locus_tag="VFY44_00580"
     CDS             807..2309
                     /locus_tag="VFY44_00580"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEX5908953.1"
                     /translation="MLRAYLSRTLVGAAAVLPVPVVAGDVPDPSAIYDNGQHVAVVTA
                     NGWAPSFRMYTSTDRSQWTLRGQVFKNPPKWVAVNRWAPEITKLSEGRYAVYYSARSK
                     RGRDPWFCIGAAFAKSATGPYKDLGKPIRCGEEGSIDPSVLRDENGRRTLLFKNDGNE
                     FQRPTSIWAQAMSEDGTSVSGKAKVIMRNDTKWEGGVIEGPSIVRSGGYFHMLYSGGL
                     FGGTVGCDYALGTARSRTLMGDWEKFPGNPILKGGNGWSCPGHGSIYPGPSGTLDALY
                     HAYPQGKGRLAGRQMIADTAFFSADNWLKIGTGTPPPRSPSAPTLAFKETFRGRLNAN
                     WEWPFMRKPGIRVNRGLRLTGSPQGRDRFDAGVIARRTATANYTGGAVLDRAGLRGKA
                     RGGIASYRNDFEGIGLSVGRDEMTVWQRNRGKLKILARGKSPDAGVVHLRMRARGNVA
                     TFQVSTDGMVWRTVGRAFRGPVYESARFALTAGGVRRGSAVFRSASVSEN"
     gene            complement(2296..>4425)
                     /locus_tag="VFY44_00585"
     CDS             complement(2296..>4425)
                     /locus_tag="VFY44_00585"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HEX5908954.1"
                     /translation="YYLGGWTRADRIAQGQHTRLFSRAMVLERQGRKFALVQVDLFMV
                     PGGMVKQIGEILAKRGFSERNILISASHTHSGPGGYANFPTLNTAAPSLQTATDPLSF
                     ARFLSPDKANPLLYTFLTRQIATAIRRADDDRAMATAGWGSSRIMGLTQNRSIEAHLA
                     NHGIVKDRGTGKESDDPGGYEHTIDPNVDVLRVDKVRRAKKGRRLKRIPIGAWSTFAD
                     HGTVTKSSFQYYNADHHASAMRVFEERVRSAAKVPARQEVLNVYGNSNEGDMSAGLTR
                     DGPAASDYVGRVEAAAMYDAWRAAGRKSSRTPAFSSRWTRVCFCGQVVEQGQQVASQS
                     QVGIPFLTGSEEERGPLFDITGEELEDRRTADAGPPHGVKASVPGVGAGVPNAVPLVA
                     FRLGRRMIVSLPAEGTKEMGARIKSAVRSQIAGSGIGDVVLSGLANEFILYLTTPQEY
                     DRQHYEGGNTQFGRQSGNLLKNEIAGLAGRLARNQAAPDPYAFDPTNGVAPTDADYGP
                     GAASASVLSQPRPAYARLQRAELSWQGGTLGLDRPVDKAFVTAQRLTRKGWKSEDSDL
                     GLAMLWSVDDRGRYRVRWEIPRTAPRGTYRLLVTGKRYQLASNRFRVFGSSALKVTRV
                     DAPAGKYTVALAYPAAVRDVDLTFRPRFADGGRVRFKVGSRTVTVKRKSGIYFSVKAA
                     AGTPVTVAPLAARDRNNNANGSAFSSR"
BASE COUNT          592 a         1620 c         1574 g          639 t
ORIGIN      
        1 cgcggtgcgg ggcgggcgaa ctcggcggtg gtcgtggaca acacgcggca ctccacgccc
       61 gcgtcctcga agatggccat cgcgaagtcg taccagctgc actcgccctg cgcggacatg
      121 tggtgcagcc cgtaggcgtc cgtgtccagc aggcgcacga tcccggcggc caggtggccc
      181 gtccaggtgg ggctgccgac ctggtcgcgc accaccacca cctgcttgtc gcgctcggcg
      241 atctcgagca tggtctccac gaagttgcgg ccgcccgtgc cgaacagcca ggacgtgcgc
      301 accaggaagt ggcgcgggtt ggcggcggcg gtgtccacct cgccggccag cttggtgcgg
      361 ccgtacaccg actcgggcga cggctcgtcg gactccacgt acgggcgggt gccggtgccg
      421 tcgaacacgt agtccgtgga ggggtagacc acgctggcgc ccacctcggc ggcccctgcg
      481 gccacgatcc ccgcggcgcc ggcgttcagg cgcatggcgg cgtcggcgtc gtcctcggcg
      541 gcgtcgaccg ccgtgtaggc ggcgcagttg accaccgcgg ccggccgctc gcgctccatc
      601 gcccggcgca cggcgcgctc gtcggacacg tccagggcgt cccggtccag ggccgcgacc
      661 tcatggttgg tgaagcgggc ggcgcggacc acgtcctggc ccagcattcc cccggctccg
      721 cacaccagca gtttcacgcc gcgcagcatg gcaggacgcc gaatcgatgc gcctgtgacc
      781 gggttaggcg ggctacagtg agcggcgtgc tgcgggcata tctttctcgg acgctggtag
      841 gggcggccgc ggtcctcccc gtcccggtgg tggccgggga cgtgcccgac ccctccgcga
      901 tctacgacaa cggccagcac gtggccgtgg tcacggccaa cggctgggcc ccgtcgttcc
      961 gcatgtacac ctccaccgac cgcagccagt ggacgctgcg ggggcaggtg ttcaagaacc
     1021 ccccgaagtg ggtggccgtg aacaggtggg cgcccgagat caccaagctc tccgagggcc
     1081 gctacgccgt gtactacagc gcccgctcga agcgcggccg cgacccgtgg ttctgcatcg
     1141 gcgccgcctt cgcgaagtcg gccaccgggc cctacaagga cctgggcaag cccatccgct
     1201 gcggcgagga gggctcgatc gacccctccg tgctgcgcga cgagaacggt cgccgcacgc
     1261 tgctgttcaa gaacgacggc aacgagttcc agcgccccac ctcgatctgg gcgcaggcca
     1321 tgagcgagga cggcaccagc gtgtccggca aggccaaggt gatcatgcgc aacgacacca
     1381 agtgggaggg gggcgtgatc gaggggccga gcatcgtgcg ctcgggcggc tacttccaca
     1441 tgctctactc gggcggcctg ttcggcggca ccgtgggctg cgactacgcc ctgggcaccg
     1501 cccgctcacg cacgctgatg ggcgactggg agaagttccc gggcaacccg atcctcaagg
     1561 gcggcaacgg ctggagctgc ccgggccacg gctccatcta cccgggcccg agcggaacgc
     1621 tcgacgcgct ctaccacgcc tatccccagg gcaagggccg gctcgcgggc cgccagatga
     1681 tcgccgatac cgcgttcttc tcggccgaca actggctgaa gatcggcacc ggcaccccgc
     1741 cgccgcgctc gcccagcgcg cccacgctgg cgttcaagga gaccttccgc gggcgcctga
     1801 acgccaactg ggagtggccg ttcatgcgca agcccggcat ccgggtcaac cgcggcctgc
     1861 ggctgacggg ttcgccgcag gggcgcgacc gcttcgacgc cggcgtgatc gcccgtcgca
     1921 ccgccacggc caactacacc ggcggcgccg tgctggaccg ggccgggctc cgcggcaagg
     1981 cgcggggcgg gatcgcctcc taccgcaacg acttcgaggg catcggcctc tcggtcgggc
     2041 gcgacgagat gacggtctgg cagcgcaacc gcggcaagct gaagatcctg gcgcgcggca
     2101 agtccccgga cgccggcgtc gtgcacctgc ggatgcgcgc acgcggcaac gtcgccacct
     2161 tccaggtcag caccgacggc atggtctggc gcacggtggg ccgcgccttc cgcgggcccg
     2221 tctacgagtc ggcccgcttc gccctgaccg ccggcggcgt ccgccgcggc tcggcggtgt
     2281 tccgcagcgc ctcggtcagc gagaactgaa cgcagagccg ttggcgttgt tgttgcggtc
     2341 acgcgcggcc agcggtgcca cggtgaccgg cgtgccggcg gccgccttga cgctgaagta
     2401 gatgccgctc ttgcgcttga cggtcacggt cctggagccg accttgaagc gcacccgccc
     2461 gccgtcggcg aagcgggggc ggaaggtcag gtcgacgtcg cgcacggccg ccgggtaggc
     2521 cagcgcgacc gtgtacttcc cggccggtgc gtccacgcgc gtcaccttca gcgccgagga
     2581 gccgaacacg cggaagcggt tgctggccag ctggtagcgc ttgccggtga ccagcaggcg
     2641 gtaggtgccg cgcggtgcgg tccgcgggat ctcccaccgc acgcggtagc ggccgcggtc
     2701 gtcgacgctc cagagcatgg ccaggcccag gtcgctgtcc tcgctcttcc agcccttgcg
     2761 cgtcaggcgc tgggcggtca cgaacgcctt gtcgaccggc cggtccagcc cgagcgtccc
     2821 gccctgccac gacagctcgg cgcgctgtag ccgggcgtag gccgggcgcg gctgggagag
     2881 caccgaggcc gacgccgccc cggggccgta gtccgcgtcg gtgggcgcca cgccgttggt
     2941 tggatcgaag gcgtaggggt ccggggccgc ctggttgcgc gccagccgcc cggccagccc
     3001 ggcgatctcg ttcttgagca ggttccccga ctggcggccg aactgggtgt tgccgccctc
     3061 gtagtgctgg cggtcgtact cctgcggggt ggtcaggtag aggatgaact cgttggccag
     3121 gccggagagc accacgtcgc cgatgcccga gcccgcgatc tgcgaccgca ccgccgactt
     3181 gatccgcgcg cccatctcct tcgtgccctc ggcgggcagg gacacgatca tccgccgccc
     3241 cagccggaag gccaccagcg gcaccgcgtt gggcacgccc gcgcccacgc cgggcaccga
     3301 cgccttgacg ccgtgcgggg gccctgcgtc cgccgtgcgc cggtcctcga gctcctcgcc
     3361 cgtgatgtcg aacagcgggc cgcgctcctc ctcggagccg gtcaggaacg ggatgcccac
     3421 ctgggactgg ctggccacct gctggccctg ctcgacgacc tggccgcaga agcacacgcg
     3481 cgtccagcgg ctcgagaagg ccggcgtccg cgagctcttg cggcccgccg cgcgccacgc
     3541 gtcgtacatc gcggcggcct ccacgcggcc cacgtagtcg gaggcggccg ggccgtcgcg
     3601 ggtcaggccg gcggacatgt cgccctcgtt ggagttgccg tacacgttca gcacctcctg
     3661 cctggccggg accttggcgg cgctgcgcac gcgttcctcg aacacccgca tggccgaggc
     3721 gtgatggtcg gcgttgtagt actggaagct cgacttggtc acggtgccgt ggtccgcgaa
     3781 cgtggaccac gcgccgatcg ggatccgctt cagccgccgc cccttcttcg cccgccgcac
     3841 cttgtccacg cgcagcacgt ccacgttcgg gtcgatcgtg tgctcgtagc cacccgggtc
     3901 gtccgactcc ttgcccgtgc cgcggtcctt gacgatgccg tggttggcca ggtgcgcctc
     3961 gatgctgcgg ttctgcgtca gccccatgat ccgtgacgag ccccagcccg ccgtggccat
     4021 cgcgcggtcg tcgtccgcgc gccggatcgc cgtggcgatc tgcctcgtca ggaacgtgta
     4081 cagcagcggg ttggccttgt cgggcgacag gaagcgcgcg aagctcagcg ggtcggtcgc
     4141 cgtctgcagg ctcggcgcgg ccgtgttcag cgtcggaaag ttcgcgtagc ccccgggccc
     4201 cgagtgggtg tggctggccg agatcaggat gttgcgctcg gagaacccgc gcttggccag
     4261 gatctcgccg atctgcttga ccatgccgcc ggggaccatg aacaggtcca cctgcaccag
     4321 cgcgaacttg cggccctggc gctccagcac catggcgcgc gagaacagcc gcgtgtgctg
     4381 gccctgcgcg atgcggtccg cccgcgtcca cccgcccagg tagta
//
LOCUS       DASUPW010000029        10036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_698242, whole genome shotgun sequence.
ACCESSION   DASUPW010000029 DASUPW010000000
VERSION     DASUPW010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 10036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10036
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698242"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VFY44_00590"
     CDS             <1..559
                     /locus_tag="VFY44_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HEX5908955.1"
                     /translation="RFETFAITRIKGSIIDELRSLDWVPRSVRAKAREIERANAKLEH
                     KLHRAPTDQEMADELEVELEDFQESLMRISNSSVVALDELWTVSDSSGDQVSLLDTIQ
                     DESAADPAQEMDLTDMKDRLADSIARLPEREKLVVALYYYENLTLREIGEVLGVTESR
                     VSQLHTKAVLRLKSRLQGDSLSEAL"
     gene            534..2642
                     /locus_tag="VFY44_00595"
     CDS             534..2642
                     /locus_tag="VFY44_00595"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX5908956.1"
                     /translation="MTPSAKPSSGLADLGERRSDDELRRRLRQQAAVAELGREALAGA
                     GVDELAASAVEMIAVELGVPVAVLVSPVPGGSDLRFRAQVGLPGAPAVLDKLPPTVPG
                     LLTYTLRSHAAVVSPDLEHETRFTPSPVLVAEGVRSAAGVPVGVQGGPFGVLAAGSRR
                     RDDFSDEDVNFLQGMGNTLAVAVEREQAAAEREEERARLEAALDKASENEARFRELAD
                     AAPVFIWTADADGLVDFINRGWLDFTGRPEEDELGDSWALGVHPDDREAVTETWWRAF
                     RRKVPWEREYRLLGRDGEYRWIVDRGVPRFDGDRLVGYVGTATDIHQRKEIERKLSEA
                     YQRDHEVAETLQRSLLPESLPKIEGLQLEARYLPASQSAAIGGDWYDAVELEDGRIAV
                     VVGDVVGHGLRAAVVMGQMRTAFRAYALLGTSPSDTLARLNRLLMKEGGELMATALYL
                     LLDRDTGQVWYASAGHPPALVVSRDSEPRYLEGGRSVPLGTTDIAYHEAEAQIERGAT
                     VILYTDGLVERRDTSLDDRLAQLATVAGVAGGELGDVCDQILGGLLGGKRPSDDVALL
                     ALRPEPPTAGSLELRLPADPAALAQLRQRMGRFLDTAGADEQERFEITLAVSEAAMNA
                     IEHAYGPSEAEFDLSASIREGEFHAEVRDSGRWRERRSDDRGRGLAIIEALMDDVEVN
                     AQPTGTVVRMRRPMGVERPA"
     gene            2639..2983
                     /locus_tag="VFY44_00600"
     CDS             2639..2983
                     /locus_tag="VFY44_00600"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5908957.1"
                     /translation="MSGGADISVTDTGEMLVASLAGEVDMTNAAYVREELSSAVPNEA
                     HALVLDLTNARYLDSAAIEVLFELSRRLVRRRQQLRLVVPRRSPLRRLFTLIDLGSVA
                     PVHDSLETAVAV"
     gene            2994..3728
                     /locus_tag="VFY44_00605"
     CDS             2994..3728
                     /locus_tag="VFY44_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908958.1"
                     /translation="MKKELRPFHDHVTCDVCGRTILKGERTEPYLVQGGQTQTVCSLC
                     FGRAEQAGWIRESASGDVPARGPRPQERRPLLGRLRRKPAEPQAEEAPPEPVAERYEE
                     APAESEPAPEPDPPREQAPKRAARRQDPRHVRAVPTTAEVKIERALDLFNASEHQRTV
                     AGLARSLGAPYAAALPDLDQPSAVNLIVAWELSWYRFRIDLGDAHDPVGLAAKGDELT
                     DIDEELRNWNAGLDAEGRLVIGVAQQ"
     gene            3725..3928
                     /locus_tag="VFY44_00610"
     CDS             3725..3928
                     /locus_tag="VFY44_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908959.1"
                     /translation="MIYCVVPQALEDELLPKLSDYYKDDPNVEVIVDRRAQGGQPGDH
                     ESYSDRRRQRIKGTFPPIEPPSS"
     gene            3943..4344
                     /locus_tag="VFY44_00615"
     CDS             3943..4344
                     /locus_tag="VFY44_00615"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEX5908960.1"
                     /translation="MTVHVDGGARGNPGPSAVAAVASDGSGQELAERSAYIGETTNNV
                     AEYKAVLLGLELARELGATEVEVVNDSELVSRQIEGRYKVKNAGLKPLHQETMESLRG
                     FDEWSVTSVRREGNVRADELVNEALDREQGR"
     gene            4341..5492
                     /locus_tag="VFY44_00620"
     CDS             4341..5492
                     /locus_tag="VFY44_00620"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEX5908961.1"
                     /translation="MSAGELSQIPFTDLRLEQRDLDAVADVLRSGWLTMGPKTAAFEE
                     AFAAQLGAKHAVAVNSCTAALHLAYLAAGVGPGDEVVVPSFTFAATAAAVLYCGATPV
                     FADIVGPEDLGVDPDDVAAKITERTKAVACVHFAGYPAAADVLADLCADRGLALIEDS
                     AHAPSATLDGRKLGTIGLAGAFSFFSNKILAIGEGGLLATDDDDVAALARSRRSHAMT
                     SGTWDRHSGRTDTYDVVGLGFNYRIDEPRSALALSRLEGLEEDIETRRGLVREYRSRL
                     GGLDGLIVPYRDQDVAGSSCYVMPVMLEDAGRRDAFRSHLSEKHGVQTSIFYPAIHEF
                     TAYSGRYPAEGLERTERAARSEVTLPLYPHMTPEEQDRVIAGVEEALTA"
     gene            5489..6622
                     /locus_tag="VFY44_00625"
     CDS             5489..6622
                     /locus_tag="VFY44_00625"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX5908962.1"
                     /translation="MSWRYPLSTLPMPEDDVQAVMACLQEGWLTMGPRTQAFEGALAQ
                     ELGAASVACVASGTAALHLACRAAGLGPGDEAIVPSMTFVATAGAVRYCGAEPVFCDV
                     ASAQAPNLDVAAVEAKITDRTKAVMAVHFCGYPADVVALRDLCDQRGLILIEDAAQAI
                     HAVAGPGGATAGTVGHLGCLSFFSKKQLCVGEGGAVITADPDMEARVRSLRSHAMTSV
                     TWDRHRGYAQSYDVVDVGYNFRMDEPRAALGLSRLPRLAGEIERRRAVAAGYRERLAG
                     VDGIELMWDDDAVRAASHFAFPVLAADREARDRARDQLKDAGIQTTWYPAVHRFTEYR
                     DRDGWEPLAASEEVAERHFCLPMSPTLEDADLDAIAEEVARVG"
     gene            6615..7697
                     /locus_tag="VFY44_00630"
     CDS             6615..7697
                     /locus_tag="VFY44_00630"
                     /inference="COORDINATES: protein motif:HMM:NF013255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5908963.1"
                     /translation="MAEVEHGLMTGFPGFIGRRLARKLLEESDDARLTVVVEKRMQPA
                     AAELAEPWGDRLEVVVGDIGERRLGLGDDDWDRLTGSVDTVFHLAAIYDLAVPLALAQ
                     RVNVDGTGNVIDFCRAASGLRRHVYVSTAYVAGRRRGTVYEHELVLGQDFKNHYESTK
                     FQAEVWVRESMADVPTTILRPAIVVGDSKTGETEKFDGPYYLLRAISQAAARGQAPMQ
                     FGRSDAPFNVVPVDYVVDGMAAAATGEDMAGETLHLVDPEPLSAHDLLETLSQEYAGR
                     ATRGRLPAWMVSNALRLTTIRKLFSDTPRESIAYLNHPVTFDTRRAVELLEPHGLHPP
                     VFPDYAAAMVSFFKEHEDDPAYMPKH"
     gene            7697..8644
                     /locus_tag="VFY44_00635"
     CDS             7697..8644
                     /locus_tag="VFY44_00635"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEX5908964.1"
                     /translation="MGPDRVTVRELWRYPVKSMVGERVGSAPVGERGIHADRLWAVRE
                     VERGATTTARRLPALLQCSARYAEEPPADAGPGNAAEAVITFPDGEELSTADPRAGAL
                     LTDLVGRKVELRPLPPETEKARFRAPLLTKSEIRHQFDLADDEELPDFSMFPVHKLAQ
                     LARNATPVGTYVDAYPLHLMTVGSLDAMRELTPEADFDVRRFRPNVVIAGSGEFDWCG
                     GRLSGPDAELEPEIPTVRCSVPVREQPGLPAQPDVMRTIKRHSDRCLGVYANVARAGR
                     LSEGDELTYGGRAQGGAARELGSRVRRSVVRASLKLMPR"
     gene            complement(8641..>10036)
                     /locus_tag="VFY44_00640"
     CDS             complement(8641..>10036)
                     /locus_tag="VFY44_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX5908965.1"
                     /translation="SSDQAPDYRGAGPFSEPESEAVRRLVATRNVTMLITNHTSGRLV
                     LRPPGVRAQGTVPDEAALRALGDRMAAAMGYTSERSWELYDNSGTTEDWSYGVTGAYG
                     YTIESSDQNFHEAYEKAVVGEYLRIGGGGNREAFLRALEAAADPASHAVIEGFAPPGR
                     TLRLTRTVGSRTGPVCLSQPTGFFSTDCGATSAPLSFSDQLAVTRRVGGDGVVGWHVN
                     PSVSPLARQSGPWELTCADASGRVLERRAVPVRRGGSVRVFLRCGPPSCVTARAVRSL
                     AVRTGAAARVRLRLRKGARAPRIRVLRAARGRLRVAARPTLRRGRAAWRPRRDGTYVL
                     EAKARSGRRTQVLRRSLVVRRGRVARGPAFARTDGCRELRVARVSTPQLARSGPRARL
                     LLRSLADVTARVRVSRGRRTLERRTLTLRGGRLTRLALPRAKGGRVTVTVATARGTAA
                     LPRRSAVRLRVRGR"
BASE COUNT         1393 a         3685 c         3718 g         1240 t
ORIGIN      
        1 ccgcttcgag accttcgcca tcacccgcat caagggctcg atcatcgacg agctgcgctc
       61 gctggactgg gtgccgcggt cggtgcgcgc caaggcgcgt gagatcgagc gcgccaacgc
      121 caagctcgag cacaagctgc accgcgcccc caccgaccag gagatggccg acgagctcga
      181 ggtggagctg gaggacttcc aggagtcgct gatgcgcatc tccaactcgt ccgtggtggc
      241 gctggacgag ctgtggacgg tctccgactc ctcgggcgac caggtgtcgc tgctggacac
      301 gatccaggac gagagcgccg ccgaccccgc gcaggagatg gacctgaccg acatgaagga
      361 ccggctggcc gactcgatcg cccggctgcc cgagcgcgag aagctggtcg tggcgctcta
      421 ctactacgag aacctgaccc tgcgcgagat cggcgaggtc ctgggggtga ccgagtcgcg
      481 cgtgtcacag ctgcacacca aggccgtgct gcgtctcaag agcaggctcc agggtgactc
      541 cctcagcgaa gccctctagc gggctcgccg acctcggcga gcgccgctcc gacgacgagc
      601 tgcggcgcag gctgcgccag caggcggcgg tggccgagct ggggcgcgag gcgctcgccg
      661 gcgccggcgt ggacgagctg gccgcgagcg cggtcgagat gatcgccgtg gagctgggcg
      721 tgccggtggc cgtgctggtc tcgcccgtgc ccggcggctc ggacctgcgc ttccgcgccc
      781 aggtgggcct cccgggcgcg cccgcggtgc tggacaagct cccgcccacg gtgccgggcc
      841 tgctgaccta cacgctgcgc tcgcacgccg cggtggtctc gcccgacctg gagcacgaga
      901 cgcgcttcac cccgtcgccg gtgctggtgg ccgagggcgt gcgcagcgcc gccggggtgc
      961 cggtgggcgt gcagggcggg ccgttcggcg tgctggcggc gggcagccgc cgccgcgacg
     1021 acttctccga cgaggacgtg aacttcctcc agggcatggg caacaccctg gccgtggcgg
     1081 tcgagcgcga gcaggccgcc gccgagcgcg aggaggagcg cgcgcggctt gaggcggcgc
     1141 tggacaaggc gtccgagaac gaggcccgct tccgcgagct ggccgacgcg gcgccggtgt
     1201 tcatctggac cgccgacgcc gatgggctcg tggacttcat caaccgcggc tggctggact
     1261 tcaccggccg gcccgaggag gacgagctgg gcgacagctg ggcgctgggg gtccaccccg
     1321 acgaccgcga ggccgtgacc gagacctggt ggcgggcctt ccggcgcaag gtgccgtggg
     1381 agcgcgagta ccggctcctg ggccgggacg gggagtaccg ctggatcgtc gaccgcggcg
     1441 ttccccggtt cgacggcgac cggctggtgg gctacgtggg caccgcgacc gacatccacc
     1501 agcgcaagga gatcgagcgc aagctcagcg aggcctacca gcgcgaccac gaggtggccg
     1561 agaccctgca gcggtcgctg ctgcccgaga gcctgcccaa gatcgagggg ctccagctgg
     1621 aggcgcgcta cctcccggcc agccagagcg ccgccatcgg cggcgactgg tacgacgcgg
     1681 tcgagctcga ggacggccgg atcgcggtgg tggtgggcga cgtcgtcggc catggcctcc
     1741 gggccgccgt ggtcatgggc cagatgcgca cggccttccg cgcctacgcg ctcctgggca
     1801 cctcgccgtc ggacacgctg gcgcgcctga accggctgct gatgaaggag ggcggcgagc
     1861 tcatggccac cgcgctctac ctgctgctgg accgcgacac cgggcaggtc tggtacgcca
     1921 gcgcggggca cccgcccgcg ctggtggtct cccgcgacag cgagccgcgc tacctggagg
     1981 gcgggcgctc cgtgccgctg ggcaccaccg acatcgccta ccacgaggcc gaggcgcaga
     2041 tcgagcgggg cgcgacggtg atcctgtaca ccgacggcct ggtcgagcgc cgcgacacct
     2101 cgctcgacga ccggctggcc cagctggcca ccgtggccgg cgtcgccggc ggcgagctcg
     2161 gcgacgtgtg cgaccagatc ctgggcggcc tgctgggcgg caagcgcccc agcgacgacg
     2221 tggcgctgct ggccctgcgc ccggagccgc ccacggccgg ctcgctggag ctgcggttgc
     2281 ccgcggaccc cgcggcgctg gcccagctgc gccagcgcat gggccggttc ctggacacgg
     2341 ccggcgccga cgagcaggag cgcttcgaga tcacgctggc cgtctccgag gccgcgatga
     2401 acgccatcga gcacgcctac ggccccagcg aggccgagtt cgacctgagc gccagcatcc
     2461 gcgagggcga gttccacgcc gaggtgcgcg attcgggccg ctggcgcgag cgccgaagcg
     2521 acgatcgggg acgcggattg gcgattatcg aagccctgat ggatgatgtc gaggtgaacg
     2581 cccagccgac aggcacagtc gtccgcatgc gtcggcccat gggcgtggag cgccccgcgt
     2641 gagcgggggc gccgatatct cggtcaccga caccggggag atgctcgtgg cgtcgctggc
     2701 cggcgaggtg gacatgacca acgccgccta cgtgcgcgag gagctgagca gcgcggtgcc
     2761 caacgaggcc cacgccctgg tgctggacct gaccaacgcg cgctacctgg attccgcggc
     2821 catcgaggtc ctgttcgagc tctcccgccg gctggtccgg cggcgccagc agctgcgcct
     2881 ggtcgtaccc cggcgctcgc cgctgcggcg gctgttcacg ctgatcgacc tgggcagcgt
     2941 ggctcccgtg cacgactccc ttgagacggc cgtagccgtc tagtatcgcc ggggtgaaga
     3001 aggaacttcg ccccttccat gatcacgtga cctgcgacgt ctgcgggcgc acgatcctga
     3061 agggggagcg caccgagccg tacctggtcc agggcggcca gacgcagacc gtctgctcgc
     3121 tctgcttcgg ccgcgccgag caggccggct ggatccgcga gtcggccagc ggcgacgtgc
     3181 ccgcgcgcgg cccgcggccg caggagcgcc ggccgctgct gggccgcctg cgccgcaagc
     3241 cggccgagcc gcaggccgag gaggccccgc ccgagcccgt cgccgagcgc tacgaggaag
     3301 cccccgccga gagcgagccg gcgcccgagc ccgacccgcc gcgcgagcag gcgcccaagc
     3361 gggcggcgcg ccgccaggac ccgcgccacg tgcgcgcggt gcccaccacc gccgaggtca
     3421 agatcgagcg cgccctggac ctgttcaacg cgtccgagca ccagcgcacg gtggccgggc
     3481 tggcccgcag cctgggcgcc ccctacgccg ccgcgctgcc cgacctggac cagcccagcg
     3541 ccgtgaacct gatcgtggcc tgggagctgt cctggtaccg gttccggatc gacctcggcg
     3601 acgcccatga ccccgtcggc ctggccgcga agggcgacga gctgaccgac atcgacgagg
     3661 aactccgcaa ctggaacgcc gggctcgacg ccgaaggccg actggtgata ggagttgccc
     3721 agcaatgatc tactgcgtgg tcccacaggc actcgaggac gagctccttc ccaagctctc
     3781 cgactactac aaggacgacc ccaacgtgga ggtcatcgtg gaccggcgcg cccagggcgg
     3841 ccagccgggc gaccacgagt cgtactccga tcgccgccgc cagcggatca agggcacgtt
     3901 ccccccgatc gaaccgcctt cgagctgatc gcactggatc gcgtgaccgt ccatgtggac
     3961 gggggcgcac gcggcaaccc cggcccgtcc gcggtggccg ccgtggcctc cgacggatcg
     4021 gggcaggagc tggccgagcg cagcgcctac atcggcgaga ccaccaacaa cgtggccgag
     4081 tacaaggccg tcctgctggg gctcgagctg gcgcgcgagc tgggcgccac cgaggtcgag
     4141 gtggtcaacg actccgagct ggtctcgcgc cagatcgagg gccggtacaa ggtcaagaac
     4201 gccgggctca agccgctcca tcaggagacg atggagagcc tgcgcgggtt cgacgaatgg
     4261 tcggtgacca gcgtcaggcg cgagggcaac gtgcgcgcgg acgagctggt gaacgaggcg
     4321 ctggacaggg agcagggccg atgagcgccg gcgagctgtc ccagatcccg ttcaccgacc
     4381 tgcgcctgga gcagcgggac ctggacgccg tcgccgacgt gctgcgctcg ggctggctga
     4441 cgatggggcc caagaccgcc gccttcgagg aggccttcgc cgcccagctc ggggccaagc
     4501 acgcggtggc cgtgaacagc tgcaccgccg ccctgcacct ggcctacctg gctgccgggg
     4561 tggggccggg cgacgaggtc gtggtgccct ccttcacgtt cgccgccacc gccgccgcgg
     4621 tcctgtactg cggcgcgacc ccggtgttcg ccgacatcgt cggcccagag gacctcgggg
     4681 tcgaccccga cgacgtggcc gccaagatca ccgagcgcac gaaggccgtg gcctgcgtgc
     4741 acttcgccgg ctacccggcg gcagcggacg tcctggccga cctgtgcgcc gaccgcgggc
     4801 tggcgctgat cgaggactcc gcgcacgccc ccagcgccac gctggacggc cgcaagctcg
     4861 gcacgatcgg gctggcgggg gcgttctcgt tcttctcgaa caagatcctg gccatcggcg
     4921 agggtgggct gctggccacc gacgacgacg acgtggcggc cctggcccgc tcgcgccgct
     4981 cgcacgccat gacgtcgggc acctgggacc gccactcggg gcgcaccgac acctacgacg
     5041 tggtcggact gggcttcaac taccgcatcg acgagccgcg ctccgcgctg gccctgtcgc
     5101 gcctggaggg cctggaggag gacatcgaga cccgccgcgg cctggtgcgc gagtaccgct
     5161 cgcggctcgg cggcctggac ggcctgatcg tgccctaccg cgaccaggac gtggcgggct
     5221 cgtcctgtta cgtgatgccc gtgatgctcg aggacgccgg ccgccgcgac gccttccgca
     5281 gccacctgag cgagaagcac ggcgtccaga ccagcatctt ctacccggcc atccacgagt
     5341 tcacggccta cagcggccgc tacccggccg agggcctgga gcggaccgag cgcgccgccc
     5401 gctcggaggt caccctgccg ctgtacccgc acatgacgcc cgaggagcag gaccgcgtga
     5461 tcgcgggcgt cgaggaagcc ctgaccgcat gagctggcgc tatcccctgt ccacgctgcc
     5521 gatgcccgag gacgacgtcc aggccgtgat ggcctgcctg caggagggct ggctgaccat
     5581 gggcccccgc acccaggcgt tcgagggcgc gctggcccag gagctcgggg ccgcctcggt
     5641 ggcctgcgtg gccagcggca cggcggcgct gcacctggcc tgccgcgccg cagggctcgg
     5701 gccgggcgac gaggccatcg tgccctccat gaccttcgtg gccaccgccg gcgccgtgcg
     5761 ctactgcggc gccgagcccg tgttctgcga cgtggcctcc gcgcaggcgc ccaacctgga
     5821 cgtggcggcg gtggaggcca agatcaccga ccgcaccaag gcggtcatgg ccgtgcactt
     5881 ctgcggctac ccggccgacg tggtggcgct gcgcgacctg tgcgaccagc gcgggctgat
     5941 cctgatcgag gacgccgccc aggccatcca cgcggtggcc ggccccggcg gcgccacggc
     6001 cggcaccgtc gggcacctgg ggtgcctgtc gttcttctcc aagaagcagc tgtgcgtggg
     6061 ggagggcggc gccgtgatca cggccgaccc ggacatggag gccagggtgc gctcgctgcg
     6121 ctcgcacgcc atgaccagcg tcacctggga ccgccaccgc ggctacgcgc agtcctacga
     6181 cgtggtcgat gtgggataca acttccgcat ggacgagccc cgagccgccc tgggcctgtc
     6241 ccgcctgccg cgcctggccg gggagatcga gcgccgccgc gcggtcgccg cgggctaccg
     6301 cgagcgcctg gccggggtgg acgggatcga gctgatgtgg gacgacgatg cggtccgggc
     6361 cgcctcgcat ttcgcctttc cggtgctggc cgccgaccgt gaggcgcgcg accgcgcccg
     6421 cgaccagctc aaggacgccg ggatccagac cacgtggtac cccgccgtgc atcgcttcac
     6481 cgagtaccgc gaccgcgacg gctgggagcc gctcgcggcc agcgaggagg tggccgagcg
     6541 ccacttctgc ctgcccatgt cgcccacgct cgaggacgcg gacctggacg cgatcgccga
     6601 ggaggtggcg cgcgttggct gaggtcgagc acggcctgat gacgggcttc ccgggcttca
     6661 tcgggcgccg cctggcccgc aagctgctgg aggagtccga cgacgccagg ctgaccgtgg
     6721 tggtcgagaa gcgcatgcag ccggcggccg ccgagctggc cgagccgtgg ggcgaccggc
     6781 tcgaggtggt ggtgggcgac atcggcgagc ggcggctggg cctcggcgac gacgactggg
     6841 accggctgac gggctcggtg gacaccgtct tccacctggc ggccatctac gacctggcgg
     6901 tgccgctggc gctggcgcaa cgggtgaacg tggacggcac cggcaacgtg atcgacttct
     6961 gccgcgccgc ctcggggctg cgccgccacg tgtacgtgag caccgcctac gtggccggac
     7021 gccggcgcgg cacggtgtac gagcacgagc tggtgctggg ccaggatttc aagaaccact
     7081 acgagagcac caagttccag gccgaggtgt gggtgcgcga gagcatggcc gacgtgccca
     7141 cgacgatcct gcggccggcc atcgtggtgg gcgactccaa gacgggggag accgagaagt
     7201 tcgacggccc ctactacctg ctgcgggcca tctcgcaggc cgccgcccgg ggccaggcgc
     7261 cgatgcagtt cgggcgctcc gacgccccct tcaacgtggt cccggtggac tacgtggtcg
     7321 acggcatggc ggccgcggcc acgggtgagg acatggccgg cgagacgctg cacctggtcg
     7381 accccgagcc gctgagcgcg cacgacctgc tggagacgct gagccaggag tacgcgggcc
     7441 gcgccacgcg cgggcggctg ccggcgtgga tggtctccaa cgcgctgcgc ctgaccacga
     7501 tccgcaagct cttctcggac accccacgcg agtccatcgc ctacctcaac cacccggtca
     7561 cgttcgacac ccgccgggcg gtcgagctgc tggagccgca cgggctgcac ccgccggtgt
     7621 tccccgacta cgccgccgcc atggtctcgt tcttcaagga gcacgaggac gacccggcgt
     7681 acatgcccaa gcactgatgg gcccggaccg cgtcacggtt cgggagctgt ggcggtatcc
     7741 ggtcaagtcg atggtcggcg agcgggtggg ctcggcgccg gtgggcgagc ggggcatcca
     7801 cgccgaccgg ctgtgggcgg tccgcgaggt cgagcgcgga gccacgacca ccgcgcgccg
     7861 gctcccggcg ctcctacagt gctcggcccg gtacgccgag gagcccccgg ccgacgccgg
     7921 cccgggcaac gcggcggagg ccgtgatcac gttcccggac ggcgaggagc tgtcgaccgc
     7981 cgatccccgc gccggcgccc tgctgaccga cctggtgggg cggaaggttg agcttcgccc
     8041 gctgccgccg gagaccgaga aggcccgctt ccgcgccccg ctcctgacaa agagcgagat
     8101 ccggcaccag ttcgacctgg ccgacgacga ggagctgccg gacttctcga tgttcccggt
     8161 ccacaagctg gcccagctgg cccgcaacgc caccccggtg ggcacgtacg tggacgccta
     8221 tccgctgcac ctgatgacgg tgggcagcct ggacgcgatg cgcgagctca ccccggaggc
     8281 ggacttcgac gtacgccggt tccggcccaa cgtggtgatc gccggcagcg gcgagttcga
     8341 ctggtgcggg gggcgcctgt cgggccccga cgcggagctg gagcccgaga tcccgacggt
     8401 gcgctgctcg gtcccggtgc gcgagcagcc cggcctgccg gcccagcccg acgtgatgcg
     8461 cacgatcaag cgccacagcg accgctgcct gggcgtgtac gccaacgtgg cccgcgcggg
     8521 gaggctgtcc gagggcgacg agctgaccta cggcgggcgg gcccagggag gcgccgcgcg
     8581 tgagctcggc tcgcgcgtcc ggcggagcgt ggtgcgggcg tccctgaagc tgatgccgcg
     8641 ctagcggccg cgcacgcgca gccggaccgc gctgcggcgc ggcagggccg cagtgccgcg
     8701 cgcggtggcc accgtcacgg tcacccggcc gcccttcgct cgcgggagcg ccaggcgggt
     8761 caggcgccca ccgcgcagcg tcagcgttcg ccgctccagc gtcctgcgcc cgcgtgagac
     8821 gcgcacgcgc gcggtcacgt cggccagcga ccgcagcagc agccgcgcgc gcgggcccga
     8881 ccgggccagc tggggcgtcg agacccgcgc cacgcgcagc tcgcggcagc cgtccgtgcg
     8941 ggcgaaggcc gggccgcgcg cgacccggcc gcggcggacc accagcgagc ggcggaggac
     9001 ctgggtgcgc cggcccgagc gggccttcgc ctccaggacg taggtgccgt cacggcgcgg
     9061 gcgccatgcg gcccgtcccc gccgcagcgt gggccgggcg gccacccgca ggcgcccccg
     9121 cgcggcccgc aggacacgga ttcggggagc gcgggcgccc ttgcgcaaac gcagccgcac
     9181 ccgcgcggcg gcgccggtgc gcaccgccag cgagcgcacg gcgcgcgccg tcacgcacga
     9241 cggcggcccg cagcgcagga acacgcgcac cgaaccaccg cggcgtaccg gaaccgcgcg
     9301 ccgctccagc acgcgcccgg atgcgtcggc gcaggtcagc tcccacggcc cgctctggcg
     9361 cgccagcggc gacaccgacg ggttcacgtg ccagcccacg acgccgtcgc cgcccacgcg
     9421 ccgcgtcacg gccagctggt cggagaagct caatggggcc gaggtggcgc cgcagtcggt
     9481 cgagaagaag ccggtcggct ggctcaggca caccggcccg gtccgggagc cgaccgtccg
     9541 cgtcagccgc agggtccggc cgggcggcgc gaagccctcg atcaccgcgt ggctcgcggg
     9601 gtcggccgcc gcctcgaggg cgcgcaggaa cgcctcccgg ttgccgcccc cgccgatccg
     9661 caggtactcg ccgaccaccg ccttctcgta ggcctcgtgg aagttctggt cgctggactc
     9721 gatggtgtag ccgtacgcgc cggtcacgcc gtagctccag tcctcggtcg tgcccgagtt
     9781 gtcgtagagc tcccacgacc tctccgacgt gtagcccatc gccgcggcca tccggtcccc
     9841 cagggcgcgc agggccgcct cgtcgggcac cgtgccctgg gcgcgcacgc cgggcgggcg
     9901 cagcaccagg cggccgctgg tgtggttggt gatcagcatg gtcacgttgc gggtggccac
     9961 cagccgccgc acggcctccg actcgggctc gctgaacggc ccggcgccgc ggtagtcggg
    10021 cgcctggtcc gagctg
//
LOCUS       DASUPW010000030         3288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1390790, whole genome shotgun sequence.
ACCESSION   DASUPW010000030 DASUPW010000000
VERSION     DASUPW010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390790"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VFY44_00645"
     CDS             <1..367
                     /locus_tag="VFY44_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908966.1"
                     /translation="ARAASAALDQLAASVPAPIVSMSLRAWPPDFPADVAVQRRAPYE
                     SRADSVMYRQVLAELARARGWAVHTYDAGDVERRAANLLGDRAHEVLHGPRAILGPPW
                     AKDHRVALAAAIMAGAGEP"
     gene            complement(342..1001)
                     /locus_tag="VFY44_00650"
     CDS             complement(342..1001)
                     /locus_tag="VFY44_00650"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEX5908967.1"
                     /translation="MSQRRGYGDPCGIARGLDVVGTRWALLVVRELLLGPKRFGDLRA
                     GLPGMSAETLGQRLRQLDEEGVVRHTTLPPPASVPVYELTDWGYDLRPVILALGRWGS
                     HAPFPPGDAPLSPDAMMLALPTMFDPDRAEGVDATYGVRLGEHAFRVRVEGGELEMAR
                     GDAGDADALIATDTTTLPELLWRDLPVERAEDAGTLEIEGSRRAARRFLGLFRARRPR
                     P"
     gene            1067..1873
                     /locus_tag="VFY44_00655"
     CDS             1067..1873
                     /locus_tag="VFY44_00655"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5908968.1"
                     /translation="MSERDDYPAGVPCWVEGLHRDPDAAREFYGALMGWEFEGSGGEP
                     EYFVARLRGREVAGLAPMPEAVQHADGVWMTHVAVDDAAATAEAATAAGGHVLAGPLD
                     VSPAGTLVVITDPGGAGFCAWEPEGRAGAELINEPGAWSMSVLRTGDADVADAFYGTL
                     FGWQAEPFGPPEGGAALYRLPGYVGGTPTQPVPRDVVAVRMSAEDGEAPGWALDFWID
                     DAEEAVARAAELGGTVVAGPYDAPPAFRQAVLSDPQGGAFSVSQLVPDRL"
     gene            complement(1880..2974)
                     /locus_tag="VFY44_00660"
     CDS             complement(1880..2974)
                     /locus_tag="VFY44_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908969.1"
                     /translation="MRPGSGRIVAALAVVGVALMAPATAGAATTLGQLRVIPQGVQDC
                     DPGRVYVQRTNTGGIRYQVPAGGGVITSWSVRPALNPNQQGSFQVLRDDGGNNFTVLA
                     TSAVQNLNSMENVDTFSVRFPVQGGEWIGYSFPSGSQQQCVLDAAPAGSSTWSGPLAS
                     VTTGATFTAAQTHNLLALNLQANLEPDADKDGFGDETQDRCPTQAGTTGSCSVAGGSP
                     PPADRTKPTFGSLSFSRTTFAAAKSGSAFSAQRKRRATPVGTKVSFNLSEAAAVKFTV
                     QRKTTGRRVSGRCKARTRKNRRKARCTLWKNVRGSFTYTGKAGRNSLTFRGRIGGRAL
                     RTGSYRLNGTATDPAKNASAPRNKSFRIVR"
     gene            complement(2971..>3288)
                     /locus_tag="VFY44_00665"
     CDS             complement(2971..>3288)
                     /locus_tag="VFY44_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908970.1"
                     /translation="SKVSFNLSEAASVRFTVERKTSGRRVSGKCKARTRKNRKKAKCD
                     LWKKVTGSFTVPGKAGANSFTFRGRIGGKALSKGDYRLTGTATDAARNVSVPTKKTFK
                     IVK"
BASE COUNT          400 a         1171 c         1212 g          505 t
ORIGIN      
        1 ggcccgggcg gcgtcggccg cgcttgacca gctcgccgcc tcggtgcccg caccgatcgt
       61 gtcgatgtcg ctgcgagcct ggccgccaga cttccctgcg gatgtcgcgg tccagcgccg
      121 cgcaccgtac gagagccggg ccgactcggt catgtaccgc caggtgctgg ccgagctcgc
      181 gcgcgcccgt ggctgggcgg tgcacaccta tgacgcgggg gatgtcgagc gccgggcggc
      241 gaacctcctc ggagatcggg cccacgaggt gctccacggt ccccgggcga tcctcggccc
      301 gccgtgggcg aaggatcacc gcgtggcgct ggcggcggcg atcatggccg gggccggcga
      361 gccctgaaca ggcccaggaa ccgccgtgca gcgcggcggc tgccctcgat ctcaagcgtg
      421 ccggcgtcct cggcccgctc gaccggcagg tcgcgccaca gcagctcggg cagggtggtg
      481 gtgtcggtgg cgatcagcgc gtccgcgtcg ccggcgtcgc cgcgcgccat ctccagctcg
      541 cccccctcga cgcggacgcg gaaggcgtgc tcgccgagcc gcaccccgta ggtggcgtcc
      601 acgccctccg cccggtccgg gtcgaacatg gtcggcaggg ccagcatcat cgcgtcgggg
      661 ctcagcggcg cgtcaccggg cgggaacggc gcgtggctgc cccaccggcc gagggccagg
      721 atcaccggtc gcaggtcata gccccagtcc gtcagctcgt agacgggcac gctcgccggc
      781 ggggggagcg tggtgtggcg gaccaccccc tcctcgtcca gctggcgcag gcgctggccc
      841 agggtctcgg cgctcatgcc cggcaggccg gcgcgcaggt cgccgaaccg cttgggcccg
      901 agcagcagct cccgcaccac cagcagcgcc cagcgcgtgc cgaccacgtc cagcccgcgc
      961 gcgatgccgc agggatcgcc gtatccacgc cgctgggaca tgcctccagt ctactgaact
     1021 gagtgagtag tcagattttc tgactacatg tggtacggtg cgctccatga gcgaacgcga
     1081 cgactacccc gccggagtgc cctgttgggt cgagggcctg caccgcgacc ccgacgccgc
     1141 acgcgagttc tacggcgcgc tgatgggctg ggagttcgag ggctccggcg gcgagccgga
     1201 gtacttcgtg gcccggctgc gcggccgtga ggtggccggc ctggcgccga tgccagaggc
     1261 cgtccagcac gccgacggcg tctggatgac ccacgtggcc gtggacgacg cggcggccac
     1321 cgccgaggcc gccaccgctg cgggcggcca cgtgctcgcg ggtccgctcg atgtctcgcc
     1381 ggccggcacg ctggtggtca tcaccgaccc cggaggagcc gggttctgcg cctgggagcc
     1441 cgagggccgc gcgggtgccg agctcatcaa cgagcccggc gcctggtcga tgagcgtctt
     1501 gaggacgggc gacgcggacg tggcggacgc cttctacggc acgctgttcg gctggcaggc
     1561 cgagccgttc ggcccgcccg aggggggcgc cgccctctac cgcctcccgg gctacgtggg
     1621 cggcacgccg acgcagcccg tgccccgcga cgtcgtggcc gtacggatgt ccgcggagga
     1681 cggcgaggcc ccgggctggg cgctcgactt ctggatcgac gatgccgagg aggccgtcgc
     1741 gcgggccgca gagctgggcg ggaccgtggt ggccgggccc tacgacgctc cgcccgcctt
     1801 ccggcaggcc gtcctgtccg acccgcaggg gggagcgttc tcggtcagcc agctggtgcc
     1861 ggaccggctc tgagggccgc taccggacga tcctgaacga cttgttcctg ggcgccgagg
     1921 cgttcttggc gggatcggtt gcggtgccgt tcaggcggta gcttcccgtc cggagggccc
     1981 tgccgccgat ccggccgcgg aaggtcaggc tgttcctgcc cgccttgccg gtgtaggtga
     2041 acgagccgcg gacgttcttc cagagcgtgc acctggcctt cctgcggttc ttgcgggtgc
     2101 gcgccttgca cctgcccgac accctgcggc cggtggtctt gcgctggacc gtgaacttga
     2161 ccgccgcggc ctcggagagg ttgaacgaca ccttggtgcc gaccggcgtg gccctgcgct
     2221 tcctctgcgc gctgaaggcc gacccggact tggccgccgc gaacgtggtc cgcgagaagc
     2281 tcaggctgcc gaaggtgggc ttggtcctgt cggccggagg agggctcccc ccggccacgc
     2341 tgcagctacc ggtggtgccg gcctgcgtgg gacagcggtc ctgggtctcg tcgccgaagc
     2401 cgtccttgtc ggcgtcgggc tccaggttgg cctgcaggtt gagggccagg aggttgtggg
     2461 tctgggccgc cgtgaacgtg gctcccgtgg tgacgctggc cagcggcccg ctccacgtcg
     2521 agctcccggc gggcgcggcg tcgaggacac actgctgttg cgatcccgac gggaagctgt
     2581 agccgatcca ctcgcccccc tggaccggga accggacgga gaaggtgtcc acgttctcca
     2641 tcgagttcag gttctggacg gcgctcgtgg cgagcaccgt gaagttgttg ccgccgtcgt
     2701 cacgcaggac ctggaacgag ccctgctgat tcgggttcag ggccggcctg accgaccagg
     2761 aggtgatcac gccgccgccg gcgggcacct ggtaccggat gccgccggtg ttcgtgcgct
     2821 ggacgtagac cctgcccgga tcgcagtcct ggaccccctg ggggatcacg cgcaactgtc
     2881 ccagggtcgt cgcggctccg gcggtggcgg gcgccatcag cgccacgccc accaccgcca
     2941 gcgctgcgac gattctgccg gatcccggtc tcacttgacg atcttgaacg tcttcttcgt
     3001 gggcacggac acgttgcgag cggcgtccgt ggcggtgccg gtcagccggt agtcaccctt
     3061 gctcagggcc ttgccgccga tgcggccgcg gaacgtgaac gagttcgcgc cggccttgcc
     3121 ggggacggtg aacgacccgg tgaccttctt ccacagatcg cacttggcct tcttgcggtt
     3181 cttgcgcgtc cgggccttgc atttcccgct gacgcggcgg ccgctggtct tgcgctcgac
     3241 cgtgaaccgc acggaggcgg cctcgctgag gttgaacgag accttgct
//
LOCUS       DASUPW010000031         2037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1113789, whole genome shotgun sequence.
ACCESSION   DASUPW010000031 DASUPW010000000
VERSION     DASUPW010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113789"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VFY44_00670"
     CDS             <1..630
                     /locus_tag="VFY44_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908971.1"
                     /translation="QRRMMALILPVIPATGFKRSVLAAAGFGTGAGGAGGIVAGGSAL
                     TGTVAKIAAVTVLAGGAGVATKEVVDRDRAAPAAETPDRPAGGPAAATPAAAGTPAPA
                     LPAAQRRQRAAEAKRRRAAATPGKARSQGKSRSNRGAAPPGQARNQAGGQGRGVPARP
                     VVPPGQLRKQQTPAAAPKPVTPRATPKAVPKLKPVKPAKPVKPGKPDNP"
     gene            complement(627..1097)
                     /locus_tag="VFY44_00675"
     CDS             complement(627..1097)
                     /locus_tag="VFY44_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908972.1"
                     /translation="MRDGRGVSEALRHLDQHGAEPDSAVVADALARFLVARCESHHKG
                     WQVVRQVVVDSSADAPWEKCARRAIPETARDLQDLSVTRGRTPLHRAQHVAARNRAQQ
                     IAGHVDQRAVLGDLGLRQRTVSDERWCRALGSAVGAKSREQVARAVEKSLAARD"
     gene            complement(1161..>2037)
                     /locus_tag="VFY44_00680"
     CDS             complement(1161..>2037)
                     /locus_tag="VFY44_00680"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEX5908973.1"
                     /translation="DTLHMWAQIVGKVRLAKAPMVNHWWQATTYVTPRGLTTGSIPDG
                     RRAFEMEFDFCAHELVVQVQGGDRRAVVLEPKTTAQFYAETMAALEELGIDVSIRPVP
                     VEVEKAIPFAEDTVHASYDAEAARLFWGQLVQADRVLREFRTRFTGKVSPVHVFWGAL
                     DMAVTRFSGHRAPRHPGGAPNCGDWVMVEGYSHELSSAGFWPGGGGEGHFYSYAYPEP
                     DGYRDHPVEPDAAFYSQDAGQYLLGYREVRESADPDATLLAFLQSTYEAVADHGEGWD
                     RAALEYRPVEGARPD"
BASE COUNT          258 a          774 c          772 g          233 t
ORIGIN      
        1 cagcgccgga tgatggccct gatcctgccc gtgatcccgg ccaccggctt caagcgcagc
       61 gtgctggccg cggccggctt cggcaccggg gccggcggcg ccggcggcat cgtggccggt
      121 gggtcggcgc tgaccggcac cgtggccaag atcgccgcgg tcacggtcct ggccggcggg
      181 gccggcgtgg ccaccaagga ggtcgtggac cgcgaccgcg cggcgcccgc ggcggagacg
      241 ccggaccggc ccgcgggcgg tccggccgcc gcgacaccgg cggccgcggg cacccctgcc
      301 cccgccctgc cggccgcgca gcgacggcag cgcgccgccg aggccaagcg caggcgcgcc
      361 gcggcgacgc ccggcaaggc ccggtcgcag ggcaagagcc gcagcaaccg tggcgccgct
      421 ccccccgggc aggcgcgcaa tcaggccggg ggccagggcc gaggcgtccc cgccaggccg
      481 gtggtcccgc cgggccagct caggaagcag cagacgccgg cggcggcccc caagccggtc
      541 acgccgcggg cgacgcccaa ggccgtgccg aagctgaagc cggtgaagcc ggccaagccg
      601 gtgaagcccg ggaaaccgga caacccctag tcgcgggcgg ccagggactt ctcgacggcg
      661 cgcgcgacct gctcgcgcga cttggccccg accgcgctcc ccagcgcccg gcaccagcgc
      721 tcgtcgctga cggtgcgctg gcgcaggccc aggtcgccca ggacggcacg ctggtccacg
      781 tgcccggcga tctgctgcgc gcggtttcgc gcggccacgt gctgggcccg gtgcaacggg
      841 gtgcgccccc gggtcaccga gaggtcctgg aggtcgcggg cggtctcggg tatcgcgcgg
      901 cgcgcgcact tctcccacgg ggcgtcggcc gacgagtcga ccacgacctg gcgcaccacc
      961 tgccagccct tgtggtggga ctcgcaccgt gcgaccagga agcgggccag ggcgtcggcc
     1021 acgacggccg agtcgggctc ggcgccgtgc tggtccaggt ggcgcagcgc ctcgctgacc
     1081 ccgcgcccgt cacgcaccgc gcgggccagc tgtggcggcg tgcggcgccg ctgctcgggg
     1141 gtcgatgcga tcccccaagg ctagtcgggc cgggcgccct ccaccgggcg gtactccagc
     1201 gcggcccggt cccagccctc gccgtggtcg gccacggcct cgtaggtgct ctgcaggaag
     1261 gccagcaggg tggcgtccgg gtcggccgac tcgcgcacct cgcggtagcc cagcaggtac
     1321 tggccggcgt cctggctgta gaacgccgcg tccggctcga ccgggtggtc gcggtatccg
     1381 tccggctcgg ggtaggcgta ggagtagaag tgcccctcac cgccgccgcc cggccagaac
     1441 ccggcgctgg acagctcgtg cgagtagccc tcgaccatga cccagtcacc gcagttgggc
     1501 gcgccgcccg ggtgacgcgg cgcccggtgg cccgagaagc gggtgacggc catgtccagc
     1561 gcgccccaga acacgtgcac ggggctgacc ttgccggtga agcgcgtgcg gaactcgcgc
     1621 agcacgcggt cggcctgcac aagctggccc cagaacagcc gcgccgcctc ggcgtcgtag
     1681 gaggcgtgca cggtgtcctc ggcgaacggg atcgccttct cgacctcgac cgggacgggc
     1741 ctgatcgaga cgtcgatgcc cagctcctcc agggcggcca tggtctcggc gtagaactgc
     1801 gcggtcgtct tgggctcgag caccacggcg cgccggtcgc cgccctggac ctggaccacg
     1861 agctcatggg cgcagaagtc gaactccatc tcgaacgcgc ggcgcccgtc cgggatcgag
     1921 ccggtggtca gcccgcgcgg ggtcacgtag gtcgtggcct gccaccagtg gttgaccatc
     1981 ggcgccttgg ccagccggac cttgcccacg atctgggccc acatgtgcag ggtgtcg
//
LOCUS       DASUPW010000032         4548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_975351, whole genome shotgun sequence.
ACCESSION   DASUPW010000032 DASUPW010000000
VERSION     DASUPW010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975351"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VFY44_00685"
     CDS             complement(<1..346)
                     /locus_tag="VFY44_00685"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX5908974.1"
                     /translation="MVEQDTRKATITTPSEREIRIEREFDAPRERVFAVYTDPELIPQ
                     WWGWRETTTRVDYMEPRTGGRWRFVDVGEDGSENAFQGAYREVTPPERVVQTFEWEGM
                     AGHVCVETTTFED"
     gene            complement(339..677)
                     /locus_tag="VFY44_00690"
     CDS             complement(339..677)
                     /locus_tag="VFY44_00690"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5908975.1"
                     /translation="MVQYSSLDRTLAALADPTRRAVIERLGQGNATITELAEPAGISL
                     PGMKKHVRVLEEAGLVATTKVGRARNCELAARQLDEVEAWTAMYRHMLEGRLDRLGEL
                     LERTKEQGDG"
     gene            724..1353
                     /locus_tag="VFY44_00695"
     CDS             724..1353
                     /locus_tag="VFY44_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5908976.1"
                     /translation="MKQLAEGVWQLSGFPADNINKYLVEDVLIDSGTRLDQGRVLRDL
                     EGREVGAHALTHAHFDHYGSSHAVCEQRGIPLWCGAADVEAVEAGKMVAKGGRLVPAA
                     KACRVDRALAEGDEVAGFTVLDVPGHSPGHVAFWRESDRVLICGDVFWGYNPFLMRGG
                     PRRPPTMVNPDDALNQASARRLAALEPALTCFGHGPPVRDASVLAAAAG"
     gene            complement(1355..2860)
                     /locus_tag="VFY44_00700"
     CDS             complement(1355..2860)
                     /locus_tag="VFY44_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908977.1"
                     /translation="MRYIVVSIAAAIALLGAGPASAAVTPTKRQCAALRADRTVATKV
                     ATAPGKGAPRVFAMQVKLDANQVRTTESFALKVECLLRAQVLPHRAKGRPNVVLFDEN
                     AGLLAGASGPRGAKARRIAANPGADAKCRGQAFPCRTLNLLTELGDAYARERRFYSSR
                     LKGLDKPSTPFALATDTIARTFMRAFSDAARSHDLYVVAGNMQAPFRQTRSKAAIRAL
                     RAPGTRPRSVYQATSGKVYNEVFTWGPRNVRRGGPDVLRNVVARNRKVPLTGIEQAIG
                     LAPGPNSGAAARRNLAPYRVPGTKARLGFATSLPAFRYGSPPAGTDPCSNVTEYYMRC
                     LDRLGANVVVQADANPGDWTGPDGDGIQKWQPLSWMTSTWRAVADPSVRFAYNATPML
                     TGNVGDLTFDGQTAITQRGLTRGPGCHYIGDRTWLAGEDRPDPTDEAGPKRQFLAIAP
                     WVVSDRSRDALRAVQKRLAPLSGDKLENDYVETAVAADMPLPVTANRAGCR"
     gene            2899..4110
                     /locus_tag="VFY44_00705"
     CDS             2899..4110
                     /locus_tag="VFY44_00705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX5908978.1"
                     /translation="MAFSNTTPLREEIDRVVPSRPFRVEFWDGSSLPATENGAGPTFT
                     VRSPRAVAQALAAPGQLGLGRAYVTGDLAVDDIDAVAAMLREWSPPPVETADKLKLMA
                     GAVRAAGVTLPARPPAAEFRPKGRRHTRGRDQRAVRHHYDVSNDYFRLFLDESMTYSC
                     AIFSRGATTLEEAQVAKLDLVCTKLDLRPGMRIVDIGSGWGAFAIHAAQHYGVHVTGI
                     TVSQNQADGATENAERAGVGDQVDFRVQDYRDLTGETFDAVASIGMVEHVGEERIDEY
                     TRKLASLVGPGGQVLNHGIARLRHSDPDAGDFSERYVFPDADPIHVSRIIDSFERAGM
                     PLQHAEDFRMDYAETLRHWAERFDSNLEEAERLGGPERVRVWRLYLRAARSGFETGFT
                     GLYQVRVRKPG"
     gene            4123..>4548
                     /locus_tag="VFY44_00710"
     CDS             4123..>4548
                     /locus_tag="VFY44_00710"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5908979.1"
                     /translation="MTAREFTKGVPEAAAATRPIPPFTEEHGELRESIRRFVAAELRP
                     HANEWEEAEWFPDEVFGKLAANGFLGLKYPERYGGEGGDHLHDAVFTEELAGCGSGGV
                     AAGIGAHIGIATPPVFKFGTEEQKQRFLVPAIKGERIAAL"
BASE COUNT          604 a         1625 c         1675 g          644 t
ORIGIN      
        1 tgtcctcgaa cgtggtcgtc tcgacgcaca cgtggccggc catgccctcc cactcgaagg
       61 tctggacgac ccgctccggg ggcgtgacct cgcggtaggc gccctggaac gcgttctcgc
      121 tgccgtcctc tccgacgtcc acgaagcgcc agcgcccgcc ggtgcgcggc tccatgtagt
      181 ccaccctggt cgtggtctcg cgccagcccc accactgcgg gatcagctcc gggtcggtgt
      241 agaccgcgaa cacgcgctcg cgcggggcgt cgaactcgcg ctcgatgcgg atctcgcgct
      301 cgctcggggt ggtgatcgtg gcctttcgtg tgtcctgctc aaccatcgcc ttgctccttc
      361 gtccgttcca gcagttcccc gagccggtcg aggcggccct ccagcatgtg ccggtacatg
      421 gccgtccatg cctcgacctc gtcgagctga cgtgcggcga gctcgcagtt gcgcgcccgc
      481 ccgaccttcg tggtggccac caggccggct tcctcgagca cgcgcacgtg cttcttcatg
      541 cccgggagcg agatgcccgc gggctcggcc agctcggtga tcgtcgcgtt gccctgcccc
      601 agccgctcga tcacggcccg gcgcgtggga tccgcgaggg ccgcgagggt gcggtcgagg
      661 gaggaatact gaaccatgtg gttcagtatt ccacagaagc cgtcggcggt ctagggtcgg
      721 cgcatgaagc agcttgcgga gggcgtgtgg cagctgtcgg ggttcccggc ggacaacatc
      781 aacaagtacc tcgtcgagga cgtgctgatc gactccggca cgcggctgga ccagggccgt
      841 gtgctgcgcg acctcgaggg ccgcgaggtc ggcgcccacg cgctcaccca cgcgcacttc
      901 gaccactacg gctccagcca cgcggtgtgc gagcagcggg gcatcccgct gtggtgcggc
      961 gccgccgacg tggaggcggt cgaggccggg aagatggtgg ccaagggcgg gcgcctggtg
     1021 cccgccgcca aggcctgccg cgtggaccgc gcgctggccg agggcgacga ggtggcgggc
     1081 ttcaccgtgc tggacgtgcc gggccactcc cccggccacg tcgcgttctg gcgcgagtcc
     1141 gaccgcgtgc tgatctgcgg cgacgtgttc tggggctaca acccgttcct gatgcgcggc
     1201 gggccgcgcc ggccgccgac gatggtgaac cccgacgacg cgctgaacca ggcgtccgcg
     1261 cggcggctgg ccgcactgga gcccgcgctc acgtgcttcg ggcacggccc gcccgtccgg
     1321 gacgcctccg tactggccgc ggctgccggc taactcagcg gcagccggcg cgattcgcgg
     1381 tgaccggcag cggcatgtcg gccgccaccg ccgtctccac gtagtcgttc tcgagtttgt
     1441 cacccgagag cggggccagg cgcttctgga ccgcgcgcag cgcgtcgcgt gagcggtcgg
     1501 acaccaccca cggcgcgatg gccaggaact ggcgcttggg cccggcctcg tcggtcgggt
     1561 cggggcggtc ctcgccggcg agccacgtgc ggtcgccgat gtagtggcac ccgggcccgc
     1621 gcgtcaggcc gcgctgggtg atggcggtct ggccgtcgaa ggtcaggtcg cccacgttgc
     1681 cggtcagcat cggcgtggcg ttgtaggcga agcgcaccga cgggtcggcc accgcgcgcc
     1741 aggtggacgt catccacgac agcggctgcc acttctggat gccgtccccg tcggggccgg
     1801 tccagtcgcc cgggttggcg tcggcctgca cgaccacgtt ggcgcccagc cggtccaggc
     1861 agcgcatgta gtactcggtc acgttcgagc acgggtcggt cccggcgggc ggtgagccgt
     1921 agcggaaggc ggggaggctg gtggcgaagc cgagcctggc cttggtgcca ggcacgcggt
     1981 agggggcgag gttgcgccga gcggccgcgc cggagttcgg cccgggcgcc aggccgatgg
     2041 cctgctcgat gccggtcagc ggcaccttgc ggttgcgggc caccacgttg cgcaggacgt
     2101 cggggccgcc gcggcgcacg ttgcgcgggc cccaggtgaa cacctcgttg tagaccttgc
     2161 cgctggtggc ctggtagacc gaccgcggcc tggtccccgg ggcccgcagc gcgcggatgg
     2221 cggccttcga cctggtctgg cggaacggcg cctgcatgtt gcccgcgacc acgtacaggt
     2281 cgtggctccg cgcggcgtcg gagaacgcgc gcatgaaggt gcgggcgatg gtgtcggtgg
     2341 ccagcgcgaa cggggtcgag ggcttgtcca ggcccttgag ccgcgaggag tagaagcggc
     2401 gctcgcgcgc gtaggcatcg cccagctcgg tgagcaggtt gagcgtgcgg cacgggaagg
     2461 cctggccgcg gcacttcgcg tcggcacccg ggttggcggc gatccgccgc gccttggcgc
     2521 cgcgcggccc cgacgcgccc gccagcagcc cggcgttctc gtcgaagagc acgacgttgg
     2581 gccggccctt ggcccggtgg ggcagcacct gcgcccgcag caggcactcc accttcagcg
     2641 cgaagctctc ggtggtccgc acctggttcg cgtccagctt gacctgcatg gcgaagaccc
     2701 gcggggcgcc cttgccgggg gccgtggcga ccttggtggc cacggtgcga tcggcgcgca
     2761 gcgccgcgca ctgccgcttg gtcggggtga ccgcggccga cgccgggccc gcgcccagca
     2821 gggcgatggc agccgcaatc gagacaacga tgtaccgcac gtggccaccc taatacggcg
     2881 acgagtaacg tgacgcgcgt ggcgttttcg aacaccacac cgctacgtga agagatcgac
     2941 cgggtcgtcc catcccggcc cttccgtgtg gagttctggg acggcagctc gctgcccgcc
     3001 accgagaacg gcgccggccc cacgttcacc gtgcgctcgc cgagggcggt cgcccaggcc
     3061 ctggccgcgc cgggccagct tgggctcggt cgcgcctacg tgaccggcga cctggccgtg
     3121 gacgacatcg acgccgtcgc cgcgatgctg cgcgagtggt cgccgccgcc ggtcgagacc
     3181 gccgacaagc tcaagctcat ggccggcgcg gtgcgcgccg ccggcgtgac gctgccggcg
     3241 cgcccgccgg ccgccgagtt caggcccaag ggccgccgcc acacccgcgg gcgcgaccag
     3301 cgcgccgtgc gccaccacta cgacgtgtcc aacgactact tccggctgtt cctggacgag
     3361 tcgatgacct acagctgcgc gatcttctcc cgcggcgcca ccacgctcga ggaggcgcag
     3421 gtggccaagc tcgacctggt gtgcaccaaa ctcgacctcc ggccgggcat gcggatcgtg
     3481 gacatcggca gcggctgggg cgcgttcgcc atccacgcag cgcagcacta cggcgtacac
     3541 gtgaccggca tcaccgtgtc gcagaaccag gccgacgggg cgaccgagaa cgccgagcgc
     3601 gccggcgtgg gcgaccaggt cgacttccgc gtgcaggact atcgcgacct gacgggcgaa
     3661 accttcgacg cggtggccag catcggcatg gtcgagcacg tgggcgagga gcgcatcgac
     3721 gagtacacgc gcaagctggc ctcgctggtg ggccccggcg gccaggtgct caaccacggc
     3781 atcgcgcggc tgcgccacag cgaccccgac gcgggtgact tctcggagcg gtacgtgttc
     3841 cccgacgcgg acccgatcca cgtctcgcgc atcatcgact cgttcgagcg cgcgggcatg
     3901 ccgctccagc acgccgagga cttccgcatg gactacgccg agacgctgag gcactgggcc
     3961 gagcgcttcg actccaacct ggaggaggcc gagcgcctgg gcggccccga gcgcgtccgc
     4021 gtgtggcgcc tgtacctgcg cgccgcccgc agcggcttcg agaccggctt caccgggctc
     4081 taccaggtgc gggtgcggaa gccgggctga gatgatgcgc gcatgaccgc gcgcgagttc
     4141 accaagggcg tccccgaggc cgccgcggcc acccggccga tcccgccgtt caccgaggag
     4201 cacggcgagc tgcgcgagtc gatccgccga ttcgtggccg ccgagctgcg cccccacgcc
     4261 aacgagtggg aggaggccga gtggttcccc gacgaggtgt tcggcaagct ggccgccaac
     4321 ggcttcctgg ggctgaagta ccccgagcgg tacggcggcg agggcggcga ccacctgcac
     4381 gacgccgtgt tcaccgagga gctggccggg tgcggctcgg gcggcgtggc cgccgggatc
     4441 ggcgcgcaca tcgggatcgc cacgccgccg gtgttcaagt tcggcaccga ggagcagaag
     4501 cagcgcttcc tggtgcccgc gatcaagggt gagcggatcg ccgccctg
//
LOCUS       DASUPW010000033         2024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_837557, whole genome shotgun sequence.
ACCESSION   DASUPW010000033 DASUPW010000000
VERSION     DASUPW010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837557"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1554
                     /locus_tag="VFY44_00715"
     CDS             127..1554
                     /locus_tag="VFY44_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HEX5908980.1"
                     /translation="MLRDRYAPQDLFALVPAATLALDPVLARLDTLLDDDDLYQRVRA
                     DLSTRYPRTTTRGRHSTPVEVILRLLLIRRLYGWSYAEVERFVNDSLVLRQFCRVYLR
                     PVPDDTTLIRWANLIGPATVAALNDRAVALAREQRVTRGRKLRIDGTVVETNIRYPTD
                     SKTLGDGVRVLSRLLRRAKRVLGEGAALGADAFRTRTRSVRRVTQRLHRVARRKGEQA
                     AEALKHAYVQLIGIAQQSRRQAQRVATALRDEATGQGQRLVERIDHFLPLVDQAIAQA
                     TRRVLRGEAVPAGEKLLSLFEPHTQAIRRHKPGKLTEFGRKVLVDEVEGGIVSHYAVL
                     DDVGLEHPHFVASLDAHQARFGHPPDLVAGDRGLYSPENEARAQERGVRRVVLPKAGR
                     ASQERQQHERQRWFRRGFRFRAGAEGRIHVLKRDYGLDRCRDHGEAGFGRWVGWGLLT
                     HNLAQIAKTQAVRATRPSASTRRAV"
     gene            1734..>2024
                     /locus_tag="VFY44_00720"
     CDS             1734..>2024
                     /locus_tag="VFY44_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908981.1"
                     /translation="MIVDMHSHYIPLGAVAAAGADVATVERGNDGRYTFRAADQVLTL
                     DPQLFELERQRAGLRRQGLDRRTLMLPPFALLYELPAAAGRRWARAVNDATAA"
BASE COUNT          293 a          743 c          734 g          254 t
ORIGIN      
        1 cggccatttc gcaccgggaa ctaacactag ttcccggtgc gaaatggccc cggaattcgg
       61 cgtcctggca cgggttctgc cgcgcggtgg tggcagaatg gctgcgctgc tcgccggagg
      121 tgcccgatgt tgcgcgaccg ctacgccccg caggacctgt tcgccctggt gccggcggcc
      181 accctggcgc tcgacccggt gctcgcgcgg ctcgatacgc tgctggacga cgacgacctg
      241 taccagcgcg tgcgggccga cctcagcacg cggtacccgc ggacgaccac gcgcggccgg
      301 cactccacgc cggtcgaggt catcctgcgc ctcctgctca tccgccggct ctacggctgg
      361 agctacgccg aagtggagcg cttcgtcaac gacagcctgg tgctgcgcca gttctgccgc
      421 gtctacctcc ggccggtgcc cgacgacacc accctgatcc gctgggcgaa cctcatcggg
      481 ccggcgaccg tggccgcgct caacgaccgc gcggtcgccc tggcgcggga gcagcgcgtc
      541 acgcgcggcc ggaagctgcg catcgacggg acggtggtgg agacgaatat ccgctacccg
      601 accgacagca agacgctcgg cgacggcgtg cgggtgctca gccgcttgct gcgccgggcc
      661 aagcgcgtgc tcggtgaggg agcggcgctg ggcgcggacg ccttccgcac ccgcacgcgg
      721 agcgtccgcc gcgtgacgca gcggctgcac cgcgtcgccc gccgcaaggg cgagcaggcg
      781 gcggaagcgc tgaaacacgc ctacgtacaa ctcatcggca tcgcgcagca gagccgccgg
      841 caggcccagc gggtggcgac ggcgctgcgg gacgaggcga caggccaggg gcagcgcttg
      901 gtcgagcgca tcgaccattt cctgccccta gttgatcagg ccatcgcgca ggcgacgcgg
      961 cgggtgctcc ggggcgaggc ggtgccggcc ggggagaagc tgctgagcct gttcgagccg
     1021 cacacccagg ccatccgccg ccacaagccc ggcaagctga ccgagttcgg gcgcaaggtg
     1081 ttagtggatg aggtggaagg cggcatcgtc agccactacg ccgtcctgga cgacgtgggc
     1141 ctggagcacc cgcacttcgt cgccagcctc gacgcccacc aggcgcgctt cgggcacccg
     1201 ccggacctgg tcgccgggga ccgggggctc tactcgccgg agaacgaggc gcgcgcccag
     1261 gagcggggcg tgcgacgggt cgtcttgccc aaggccgggc gggcctccca ggagcgccaa
     1321 cagcacgagc gccagcgctg gttccggcgc ggcttccggt tccgcgccgg ggccgagggg
     1381 cgcatccacg tcttgaagcg cgactatggg ctggatcgct gccgcgacca cggcgaggcg
     1441 ggcttcggcc gctgggtcgg ctggggcctc ctcacccaca acctggcgca gatcgccaag
     1501 acccaggccg ttcgggcgac ccgaccatcc gcgtcgaccc gccgggccgt gtgagccccc
     1561 ccatcccggt aggagccgac cgggtgcagt caaggggggc agaccgggcg gtgcgaaatg
     1621 gccatttccc caatgctggc gcgacaattc ggccatttcg caccgggaac tagcgctagg
     1681 gcgccctgcg gcgcgaacgg gccgagcgcc gtggcatcag ggaggggcgg gccatgatcg
     1741 tcgacatgca cagccactac atccccctcg gcgcggtcgc cgccgccggg gccgacgtcg
     1801 ccaccgtcga gcgggggaac gacgggcgct acaccttccg cgcagccgac caggtgctca
     1861 ccctcgaccc gcagctcttc gagctggagc gccagcgcgc cggcctgcgc cgccagggcc
     1921 tcgaccgccg gacgctgatg ctgcccccgt tcgcgctcct ctacgagctg ccggcggcag
     1981 caggccgacg ctgggcgcgg gcggtgaacg acgcgaccgc ggcg
//
LOCUS       DASUPW010000034         4835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1392312, whole genome shotgun sequence.
ACCESSION   DASUPW010000034 DASUPW010000000
VERSION     DASUPW010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392312"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VFY44_00725"
     CDS             complement(<1..655)
                     /locus_tag="VFY44_00725"
                     /inference="COORDINATES: protein motif:HMM:NF028194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX5908982.1"
                     /translation="MSDTTREIRLASRPDGRPTEQNFELAEVPLPEPGEGQALVRNEY
                     LSVDPYMRGRMRDAKSYVPPFEVGKVMEGGAVGQVVSSNSPDLSEGDWVTSQLGWREH
                     AVVDAKGALKVDPDVAPVSTALGVLGMPGFTAWLGLTEFGRPQEGETLFVSGAAGAVG
                     SMVGQLGKLRGLRVVGSAGSDEKVQLLTDELGFDAALNYKTQDLRGTLREMCPDGIDI
                     "
     gene            681..2330
                     /locus_tag="VFY44_00730"
     CDS             681..2330
                     /locus_tag="VFY44_00730"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HEX5908983.1"
                     /translation="MATDQQTEAQQLHGGRLVAKRLKAHGVSKLFTLSGGHIFSIYDG
                     CREEGIDIVDTRHEQTAAFAAEGWAKATRQVGVAALTAGPGVTNAMSALGSSLMNGSP
                     VVALGDRAPAMRWGQGSLQEIDHVPFVAPLTRSARTVESTADIPASVDAAFAAAEGPP
                     SGPAFVDFPLDHVFMESDVDADAAAARVDYLDIPAASGTERAIELLRGAERPVIMAGT
                     GLYWAHGEDALRALAEELSIPVFVNGLGRGCLPADHELFFSRARGKGLKEADVAIVVG
                     VPMDFRLGFGASFGEETKIVLLDSAEPDRKPPRDAEVELYGGIPATLDALREGAAGGP
                     ERRPWSESLRALEDEKRAGEIEELTDDRAPLHPLRLYHELKAVLDRDAVVIGDGGDFV
                     SYAGRVIDTYEPGCWMDPGPFGCLGAGPGYALAAKIANPDRQVCLLLGDGAFGFAGME
                     FDTLVRHNIPVVAVMGNNGIWALEKHPMEFLYGYSVAADLQPECRYDQVVEALGGHGE
                     LVREPGELRGALERAFSSGKPALVNVLTDPSVVYPRKSNLA"
     gene            complement(2659..3603)
                     /locus_tag="VFY44_00735"
     CDS             complement(2659..3603)
                     /locus_tag="VFY44_00735"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX5908984.1"
                     /translation="MHDPARIAVADLGTNSTRLLVAEVTSDGHVTPVERRSEVTRLGD
                     GVDASGRLSDAAMQRVADVLARYREIATEAGASRSVAVATSAVRDADNGQEFRDRVRA
                     QTGFELVVIPGEEEARLTFLGATAGHSGDEPVLVIDIGGGSTEFVIGLPGRAPGFHVS
                     TRLGSVRQSERHLASDPPEHHETEALGRAAREIVADNVPEAERSRARSAIAVAGTATS
                     LAAIDQELVPYDPDRVDGYRLGLADCERILARLASMPLADRRRVPGLHPDRAPTIVAG
                     AAILVSALQIFGLREAEVREADILHGVALDALYKGEHG"
     gene            3667..4551
                     /locus_tag="VFY44_00740"
     CDS             3667..4551
                     /locus_tag="VFY44_00740"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX5908985.1"
                     /translation="MSDFIQANTDLVTTAATVVGIILVAFVTSQLVRRSIKKALRSLS
                     QGTVKERLGARKPAMLHETGELSIRSEQRVEALATVLRSAASVVIWTIAAFLVLGQFG
                     VDLGPLIAGAGIVGVALGFGSQSLVKDFISGIFILIEDQFGVGDLVDLGEATGTVTAV
                     SLRTTRLRAIDGTVWHVPNGEIRRVGNKSQHWSRALLDIQVAYGTDIPAARAVIKQVA
                     DALWHEWPDTIIEEPEVWGVEDLGPNAIDIRMALKTKPSEQFEVGRELRERIKAAFER
                     EGIEIPNQNMVMADAAAA"
     gene            4599..4672
                     /locus_tag="VFY44_00745"
     tRNA            4599..4672
                     /locus_tag="VFY44_00745"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4633..4635,aa:Met,seq:cat)
BASE COUNT          677 a         1767 c         1714 g          677 t
ORIGIN      
        1 agatgtcgat cccgtcgggg cacatctcgc gcagggttcc gcgcagatcc tgggtcttgt
       61 agttcagggc cgcgtcgaag cccagctcat cggtcagcag ctgcaccttc tcgtccgagc
      121 cggcgctgcc caccacgcgc aggccgcgga gcttgcccag ctggcccacc atggagccca
      181 ccgcgccggc ggcgccggac acgaacaggg tctcgccctc ctggggccgc ccgaactcgg
      241 tcagccccag ccaggccgtg aagccgggca tgcccagcac cccgagggcc gtcgacaccg
      301 gcgccacgtc ggggtccacc ttcagcgcgc ccttggcgtc caccaccgcg tgctcgcgcc
      361 agcccagctg gctggtgacc cagtcgccct cgctcaggtc ggggctgttc gaggacacga
      421 cctggcccac ggcgccgccc tccatgacct tgcccacctc gaacgggggc acgtaggact
      481 tggcgtcgcg catgcgcccg cgcatgtacg ggtcgaccga caggtactcg ttgcgcacca
      541 gcgcctggcc ctcgccgggc tcgggcaggg gcacctcggc cagctcgaag ttctgctcgg
      601 tgggccggcc gtcggggcgg gaggccaggc gtatctcgcg ggtggtatcg ctcatgcccc
      661 gaccctaata gggtccgcgc atggcgaccg atcagcagac cgaggcccag caactccacg
      721 gcggccggct ggtcgcgaag cggctcaagg cccacggcgt gagcaagctc ttcaccctct
      781 cgggcgggca catcttctcc atctacgacg gctgtcgcga ggagggcatc gacatcgtcg
      841 acacccgcca cgagcagacc gcggcgttcg ccgccgaggg ctgggccaag gccacccgcc
      901 aggtcggcgt cgccgcgctg accgccggcc cgggcgtgac caacgccatg agcgcgctgg
      961 gcagctcgct gatgaacggc tcgcccgtgg tcgcgctggg cgaccgcgcg ccggccatgc
     1021 gctggggcca gggatcgctc caggagatcg atcacgtccc gttcgtcgct ccgctgacgc
     1081 gctcggcgcg gacggtggag tccacggccg acatcccggc ctcggtggac gcggccttcg
     1141 cggccgcgga gggaccgccc tcgggccccg cgttcgtgga cttcccgctg gatcacgtgt
     1201 tcatggagtc cgacgtggac gccgacgccg cggccgcgcg cgtggactac ctggacatcc
     1261 ccgccgcgtc cggcaccgag cgcgcgatcg agctgctgcg cggcgccgag cgcccggtga
     1321 tcatggccgg caccggcctc tactgggcgc acggcgagga cgccctgcgc gcgctcgccg
     1381 aggagctgtc gatccccgtg ttcgtcaacg gcctcggccg cgggtgcctg ccggccgatc
     1441 acgagctgtt cttctcccgc gcgcgcggca agggcctcaa ggaggccgac gtggccatcg
     1501 tggtcggggt ccccatggac ttccgcctgg gcttcggcgc ctccttcggc gaggagacca
     1561 agatcgtgct gctggactcg gccgagcccg accgcaagcc gccgcgggac gccgaggtgg
     1621 agctctacgg cggcatcccc gccacgctgg acgccctccg cgagggcgcg gccggcggcc
     1681 ccgagcgccg gccctggtcg gagtcgctcc gggcgctgga ggacgagaag cgcgccggcg
     1741 agatcgagga gctgaccgac gaccgcgcgc cgctgcaccc gctgcgcctc taccacgagc
     1801 tcaaggccgt gctggaccgc gacgccgtgg tcatcggcga cggcggcgac ttcgtctcct
     1861 acgccggccg cgtgatcgac acctacgagc cgggctgctg gatggacccg gggccgttcg
     1921 gctgcctggg ggcggggccg ggctacgcgc tggcggccaa gatcgccaac cccgaccggc
     1981 aggtgtgcct gctgctgggc gacggcgcct tcggcttcgc cgggatggag ttcgacaccc
     2041 tggtccgcca caacatcccg gtcgtggccg tgatgggcaa caacggcatc tgggcgctgg
     2101 agaagcaccc gatggagttc ctgtacggct actcggtcgc cgccgacctg cagcccgagt
     2161 gccgctacga ccaggtggtc gaggccctgg gcggccacgg cgagctggtg cgcgagcccg
     2221 gggagctgcg cggagcgctc gagcgggcgt tctccagcgg caagccggcg ctggtcaacg
     2281 tgctgaccga cccgtccgtg gtctacccgc gcaagtccaa tctggcctag ggactgcgcc
     2341 gtaccggcga agcggggcct ggaggacttt cacgttttgc aggtggaacg cggtccgtac
     2401 cgcttcccga ccccagaaat ggaacggccc gggtaaaccc gggccgcccc tacggctcaa
     2461 tcaggacggg caggggtacg tgccttgaaa gagccttgcg gttcggacgg cgggaggcgc
     2521 cgatacgatg cggcttcgcc cgaacctatg taccgggccc atatgggacg gctgcaagca
     2581 gctaccccgg acccagcgcc atcgatagta gccgacgccg gcgccaaacg gcaaccccga
     2641 agtggtccaa cgatcgcctc agccgtgctc ccctttgtac aaagcgtcta acgcgacgcc
     2701 gtgcaggatg tcggcctcgc gtacctccgc ctcacgcagg ccgaagatct gcagcgccga
     2761 caccaggatc gcggcgccgg cgacgatggt gggggcccgg tccgggtgca ggccgggcac
     2821 ccgccgccgg tcggccaggg gcatcgacgc cagccgggcc aggatgcgct cgcagtcggc
     2881 caggcccagc cggtagccgt cgacccggtc ggggtcgtac ggcaccagct cctggtcgat
     2941 ggccgcgagc gacgtggccg tgcccgccac cgcgatcgcg ctccgggcgc ggctgcgctc
     3001 ggcctcgggc acgttgtcgg cgacgatctc gcgcgcggcc cggcccagcg cctcggtctc
     3061 gtggtgctcg ggcgggtccg aggccaggtg gcgctcggac tggcgtaccg accccagccg
     3121 cgtcgacacg tggaagccgg gcgcccgccc cggcagcccg atgacgaact cggtgctgcc
     3181 gccgccgatg tcgatgacca gcaccggctc gtcgcccgag tgtccggcgg tcgcgcccag
     3241 gaaggtcagg cgggcctcct cctcgccggg aatgaccacc agctcgaagc ccgtctgcgc
     3301 gcgcacgcgg tcgcggaact cctgtccgtt gtcggcgtcg cgtacggcgc tggtggccac
     3361 ggccaccgag cgcgaggcgc ccgcctccgt ggcgatctcc cggtagcggg ccagcacgtc
     3421 ggccacgcgc tgcatggcgg cgtcggagag gcggccgctg gcgtcgaccc cgtcgcccag
     3481 gcgggtgacc tcgctgcgcc gctccacggg cgtcacgtga ccatcgctcg tgacctcggc
     3541 caccagcagc cgggtggagt tcgtgcccag gtcggcgacc gcgattcggg cgggatcatg
     3601 catccgtccg cacgctaccg gggaggggct gacacgtcgc ggaacggcct ctaccctgcc
     3661 gccgcgatgt ccgacttcat ccaagcgaac acggatctgg tgaccaccgc cgcgaccgtg
     3721 gtcgggatca tcctggtcgc cttcgtgacc agccagctgg tgcgcaggtc gatcaagaag
     3781 gccctgcgca gcctcagcca gggcacggtc aaggagcgcc tcggcgcccg caagccggcc
     3841 atgctgcacg agaccggcga gctcagcatc cgctccgagc agcgggtcga ggcgctggcc
     3901 acggtgctgc gcagcgccgc cagcgtggtc atctggacca tcgccgcgtt cctggtcctg
     3961 ggccagttcg gcgtcgacct cggcccgctg atcgccggcg cggggatcgt gggcgtggcg
     4021 ctgggcttcg gctcccagtc gctggtcaag gacttcatct cgggcatctt catcctgatc
     4081 gaggaccagt tcggcgtggg cgacctggtc gacctgggcg aggccaccgg gaccgtcacc
     4141 gccgtgtcgc tgcgcaccac ccggctgcgg gccatcgacg gcaccgtctg gcacgtgccc
     4201 aacggcgaga tccggcgcgt gggcaacaag tcccagcact ggtcgcgcgc cctgctggac
     4261 atccaggtgg cctacggcac cgacatcccc gccgcgcgcg cggtgatcaa gcaggtggcc
     4321 gacgcgctct ggcacgagtg gccggacacg atcatcgagg agcccgaggt gtggggggtc
     4381 gaggacctcg gccccaacgc catcgacatc cgcatggcgc tcaagaccaa gccgtccgag
     4441 cagttcgagg tcgggcgcga gctgcgcgag cggatcaagg ccgcgttcga gcgcgagggc
     4501 atcgagatcc ccaaccagaa catggtcatg gccgacgccg ccgcggcctg atccggttta
     4561 cagccccacc ccttcattgg tacccttacc ggtcgcgacg cggggtggag cagtcaggta
     4621 gctcgtcggg ctcataaccc gaaggtcgcg ggttcgaatc ccgcccccgc tactacagga
     4681 ggcccgcttg tttggcgggc cttctgcttt ttggggcggg cggaggacgc tgcaggacgc
     4741 tggcccagga aagccgccca cgaacgcgcc gagttcgcca cccgtgccgt tgagcggtgg
     4801 cgctcagtcg tggtcggtca tagggtggcg cggtc
//
LOCUS       DASUPW010000035         4955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_562297, whole genome shotgun sequence.
ACCESSION   DASUPW010000035 DASUPW010000000
VERSION     DASUPW010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562297"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VFY44_00750"
     CDS             complement(<1..212)
                     /locus_tag="VFY44_00750"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908986.1"
                     /translation="MGKLIVSKGVDLLLAAWPLVVAGVPDARLCVVGFGTYRDTLHEM
                     VEALAGADLDALRAIAARGREPEGGPA"
     gene            739..1008
                     /locus_tag="VFY44_00755"
     CDS             739..1008
                     /locus_tag="VFY44_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908987.1"
                     /translation="MSDRQTYRLRTRSDREVIREVEPGKTYVDHESGETFEVVGKTIP
                     LAPSPSALPWSVENLRLCGCSLEQLVPKDLNDCLHCGRRLPATEG"
     gene            1034..1387
                     /locus_tag="VFY44_00760"
     CDS             1034..1387
                     /locus_tag="VFY44_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908988.1"
                     /translation="MKRLLTAFACGVLLATGVAACGDDEKASKTTPTVTTETETAPTT
                     ETVPTATTPPPATTPRTTPTPPADPNSGGTTVPRTTPQSQPEDGPDNDLPPEPGSPAE
                     RFEQQCQQNPQTCGD"
     gene            complement(1398..1748)
                     /locus_tag="VFY44_00765"
     CDS             complement(1398..1748)
                     /locus_tag="VFY44_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908989.1"
                     /translation="MSAEAWIFMVGFRVFDVGLLIIWLVWFFRLRDDEDEATEEGGGD
                     GGGGSPKPKLPRTGPGPGGLRLPVGRWPSGGHLRDGGSPARTPVRRRPAPALPTVPAR
                     VRSPRSPSRVPRRA"
     gene            complement(1807..2538)
                     /locus_tag="VFY44_00770"
     CDS             complement(1807..2538)
                     /locus_tag="VFY44_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908990.1"
                     /translation="MKRILAIATLSTALLAPQAASAKIVELGGAIPKAAVSCPENCQA
                     VGRVTGYQGRGGTVAKPYVIPRKGKIIAFTVRLGKPDANQVKFFNDLYGNDPQVQLSI
                     LRRGDKKGTTLDHRLLAQSPTFKVSQFFGSAPTFTLDKPLEVRAKYIVALTVPTWAPV
                     FAVGLQRNHWWRSSRTKGKCDNVSQMASLTKLQSKKVFGCTYFTARLYYTATYVPDNK
                     PTSEAEATAAGKGSAAVGARTARGG"
     gene            complement(2587..2805)
                     /gene="infA"
                     /locus_tag="VFY44_00775"
     CDS             complement(2587..2805)
                     /gene="infA"
                     /locus_tag="VFY44_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX5908991.1"
                     /translation="MPKEEKIEMEGEILEALPNTMFKVRLDNDHEVLGHISGKMRRHY
                     IRILPGDRVKIELSPYDLDRGRITYRYK"
     gene            complement(2817..3020)
                     /locus_tag="VFY44_00780"
     CDS             complement(2817..3020)
                     /locus_tag="VFY44_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005511370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX5908992.1"
                     /translation="MATGTVKWFNDEKGFGFITPDSGERDLFVHFSGIEGDGFKTLAE
                     DSKVEYDEEDGDKGPKAVNVRKL"
     gene            complement(3071..3844)
                     /locus_tag="VFY44_00785"
     CDS             complement(3071..3844)
                     /locus_tag="VFY44_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5908993.1"
                     /translation="MSLADYARTGDVGAIIIEKPPLNLFDRSLVEALQAAVAAAADDR
                     PRALIVRAEGKVFTGGVDVKEFDDLGGPAAREFASELLALTHSIEDLPFPTLAAVHNL
                     CLTAGLELALACDLIWAGESAQFGLVERVVGVTPFMGGTQRMAERAGPARAREFVYSG
                     SPYPAADLERWGVVNRVVDDQRLHEAALEFASHLAAGPPLAHAATKRIVRAQVDGGTR
                     AADDITPAEAEHLFETEDLKRAVKTFLAEGPGKATFEGE"
     gene            complement(3891..4301)
                     /locus_tag="VFY44_00790"
     CDS             complement(3891..4301)
                     /locus_tag="VFY44_00790"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX5908994.1"
                     /translation="MSDPDCIFCKIVAGELPAEKVDEDEHTISFMDINPWTRGHALVI
                     PRNHHKNLYEVDPEDLAHTVKAAQRLALRARDALGADGVNLLNSCEAAAWQTVFHLHV
                     HVIPRYDDDPLRLPGQPQEPADGELAAVAAELRA"
     gene            4326..>4955
                     /locus_tag="VFY44_00795"
     CDS             4326..>4955
                     /locus_tag="VFY44_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908995.1"
                     /translation="MPLLDQSQLRDRIERLAAIERGSASSGEARAAELIAAELREAGA
                     EVTVEHERVHGTYWWPVGLLTGTGALAGVARGRWKAVLAGAFATAAVVDDISGGRQWF
                     RRRFLPQGMTTNVVARMGDPDAEHTVVVHAHHDAAHTGLVFDGRPPRALLKRFPALAE
                     NAKTTPPTMWGAVAGPALVAIGALLGSRRLRRTGVFLCAANVAAMTDIGV"
BASE COUNT          677 a         1767 c         1768 g          743 t
ORIGIN      
        1 gcgggcccgc cctcgggctc gcggccgcgg gcggcgatcg cccgcagggc gtcaaggtcg
       61 gcgccggcca gggcctcgac catctcgtgc agcgtgtcgc ggtaggtgcc gaacccgacg
      121 acgcacaggc gcgcgtcggg gacaccggcc accaccagcg gccacgcggc cagcagcagg
      181 tccacgccct tggacacgat gagcttgccc acgtagctca cgatcgggtc gcggcgcggg
      241 tccagggcgc gcagcgcggc gccggcgccc ggctcgccgc cccagcccgc ggtggcgccg
      301 ccctcgagcc tgccggccag ctcctcgagc gcggccgccg agggcgcggg ccaggatggc
      361 cgggcccatg atcaggtggt tggccagggc cacgtccggc cggacacggt cggccacgtc
      421 ggccacggcg tccacgttgg cccgcaggta gtgctccagc tcgtcgtcac ccagctcggg
      481 gaagggcacc gcgtcgaagc cctcgtacgt gtcggccacg tagacgggca ggatgcgccc
      541 gatgtcgggg ttgtgcaccg tgaccccgtc gggcagcccg acgtcctcgg ggtggcgctc
      601 ctggcagcag atgtgcacct cgtggcccag cgaggccagc gcccggcaca ggttcgtgtt
      661 gtagatgttg ctgccggtcc cgcgcagcag gtatccgtgg aagatcagta cgcgcatgtg
      721 gttggtagat tgccgcacgt gagcgatcgt cagacctacc gcctgcgcac ccgctcggat
      781 cgcgaggtca tccgcgaggt cgagcccggc aagacctacg tggaccacga gtccggggag
      841 acgttcgagg tcgtgggcaa gaccatcccg ctggcgccgt cgcccagcgc tctgccgtgg
      901 tcggtcgaga acctgcgcct gtgcgggtgc tcgctggagc agctggtccc caaggacctg
      961 aacgactgcc tccactgtgg ccgccgcctg ccggccacag agggctagcc ccgttccccg
     1021 cgaagcccgg ggtatgaagc gcctcctgac cgccttcgcc tgcggcgttc tactggccac
     1081 cggcgttgcc gcctgcggcg acgacgagaa ggcctcgaag accacgccca ccgtgaccac
     1141 cgagacggag accgctccga ccacggagac ggtgcccacc gccaccacgc ccccgccggc
     1201 caccacgccc cgcacgaccc ccacgccgcc ggcggacccc aactccggcg ggaccacggt
     1261 cccgcgcacc acgccgcaga gccagcccga ggacgggccg gacaacgacc ttccccccga
     1321 gcccggctct ccggcggagc gcttcgagca gcagtgccag cagaacccgc agacctgcgg
     1381 ggactagacc gccgcggtca ggcccggcgc ggaacgcgac tgggcgagcg gggcgagcgc
     1441 acgcgcgccg gcaccgtggg cagcgcgggg gccggacggc gcctcacggg cgtgcgggcg
     1501 gggctgccgc cgtcacgcag gtgaccgccg ctgggccagc gaccgaccgg gagccgcagg
     1561 ccgccgggac cggggccggt gcgcggcagc ttgggcttgg ggctgcctcc gccgccgtcg
     1621 cctccaccct cctcggtcgc ctcgtcctcg tcgtcgcgca gcctgaagaa ccacacgagc
     1681 cagatgatca acagtccgac gtcgaagact cggaagccga ccatgaagat ccatgcctcc
     1741 gcactcacag ggaaagacta ccccggtcag gtggctgcga aaagggcctt cgggagttgg
     1801 cgcggctcag ccgccgcggg cggtgcgtgc gccgactgcc gccgacccct tgccggcggc
     1861 cgtggcctcg gcctcggacg tcggcttgtt gtccggcacg taggtggccg tgtagtacag
     1921 ccgcgcggtg aagtaggtgc agccgaagac cttcttgctc tgcagcttgg tcagcgacgc
     1981 catctggctc acgttgtcgc acttgccctt ggtacgggac gaacgccacc agtgattgcg
     2041 ctggaggccg acggcgaaca cgggcgccca ggtgggcacg gtcagggcga cgatgtactt
     2101 ggcccggacc tcgagcggct tgtccagcgt gaaggtgggc gccgagccga agaactgcga
     2161 gaccttgaag gtggggctct gcgccaggag gcggtggtcc agcgtggtgc ccttcttgtc
     2221 gccgcggcgc aggatcgaga gctggacctg cgggtcgttg ccgtacaggt cgttgaagaa
     2281 cttgacctgg ttggcgtcgg gcttgcccag gcgcacggtg aaggcgatga tcttgccctt
     2341 gcgcgggatc acgtacggct tggccacggt gccgccgcgg ccctggtagc cggtcacgcg
     2401 gcccacggcc tggcagttct ccgggcagga gacggcagcc ttggggatgg cgccgccgag
     2461 ctccacgatc ttcgcggatg ccgcctgggg cgccaggagg gcggtggaca gggtcgctat
     2521 ggccagtatt cgcttcatct cagggctacc aacactgctg tacggcgcag agttgcgggt
     2581 tcctgcctac ttgtagcgat aggtgatgcg gccgcggtcg aggtcgtagg gcgacaactc
     2641 gatcttcacg cggtccccgg gaaggatgcg gatgtagtgc cgccgcatct tgcccgagat
     2701 gtggccgagc acctcgtggt cgttgtccag cctgaccttg aacatggtgt tcgggagggc
     2761 ttccaggatc tcgccctcca tctcgatctt ctcttctttc ggcatgggta tccgcgttac
     2821 agcttgcgga cgtttaccgc cttcgggccc ttgtcgccgt cttcctcgtc gtactccacc
     2881 ttggagtcct cggccagcgt cttgaacccg tcgccttcga tgcccgagaa gtgcacgaac
     2941 aagtcgcgct caccgctgtc gggcgtgatg aacccgaagc ccttctcgtc gttgaaccat
     3001 ttgaccgttc cggtcgccat cttcactcct gctgcatttg ggggactaac cagccgctag
     3061 aagctagcag tcactccccc tcaaacgtgg ccttcccggg cccctcggcc aggaaggtct
     3121 tgaccgcgcg cttgaggtcc tcggtctcga acaggtgctc cgcctcggcc ggcgtgatgt
     3181 cgtcggcggc cctggtcccc ccgtccacct gggcgcggac gatgcgcttg gtggccgcgt
     3241 gggccagcgg cgggccggcg gccaggtggg aggcgaactc cagcgcggcc tcgtgcaggc
     3301 gctggtcgtc caccacccgg ttgacgaccc cccaccgctc caggtcggcc gccgggtagg
     3361 ggctgccgct gtacacgaac tcgcgcgccc gggcggggcc cgcgcgctcg gccatgcgct
     3421 gggtgccgcc catgaacggg gtcaccccga ccacgcgctc gaccaggccg aactgggccg
     3481 actccccggc ccagatcagg tcgcaggcca gcgccagctc caggccggcg gtcaggcaca
     3541 ggttgtgcac cgcggccagc gtggggaacg gcaggtcctc gatcgagtgg gtcagcgcca
     3601 gcagctcgga ggcgaactcg cgcgcggcgg ggccgcccag gtcgtcgaac tccttgacgt
     3661 ccacgccgcc ggtgaacacc ttgccctcgg cgcgcacgat cagcgcccgg gggcggtcgt
     3721 cggcggcggc ggcgaccgcg gcctgcagcg cctcgaccag gctgcggtcg aacaggttca
     3781 gcggcggctt ctcgatgatg atcgcgccga cgtcgccggt acgcgcgtag tcggcgagac
     3841 tcatcggtgc ggtgctcgct cgatcgacga tcgagcggcg ctcctcccgg tcacgcgcgc
     3901 agctccgcgg ccacggcggc cagctcgccg tcggcgggct cctggggttg gcccggcagg
     3961 cgcagcgggt cgtcgtcgta gcgagggatc acgtgcacgt gcaggtggaa gaccgtctgc
     4021 caggcggccg cctcgcagct gttcagcagg ttcacgccgt cggcgcccag ggcgtcgcgg
     4081 gcgcgcagcg ccagccgctg ggccgccttg acggtgtggg ccaggtcctc ggggtcgacc
     4141 tcgtacaggt tcttgtggtg gttcctgggg atcaccagcg cgtggccgcg ggtccacggg
     4201 ttgatgtcca tgaacgagat cgtgtgctcg tcctcgtcga ccttctcggc gggcagctcg
     4261 cccgccacga tcttgcagaa gatgcagtcc gggtcgctca tcggccgact accctacaac
     4321 ccgcattgcc tctgctcgac cagtctcagc tccgtgatcg catcgaacgc ctggccgcca
     4381 tcgagcgcgg ctccgcctca tcgggcgagg cccgcgccgc cgagctgatc gccgccgagc
     4441 tgcgcgaggc cggcgcggag gtcacggtgg agcacgagcg cgtccacggg acctactggt
     4501 ggccggtcgg gctgctgacc gggacggggg cactggccgg ggttgcccgc ggtcgctgga
     4561 aggccgtcct ggcgggagcg ttcgccaccg ccgccgtggt cgacgacatc tccggcggcc
     4621 ggcagtggtt ccggcgccgg ttcctgcccc agggcatgac cacgaacgtg gttgctcgga
     4681 tgggcgaccc cgacgccgag cacaccgtgg tcgtgcacgc ccaccatgac gccgcgcaca
     4741 cgggcctggt gttcgacggc cggcccccgc gggcgctgct gaagcgcttc ccggcgctgg
     4801 ccgagaacgc caagaccacg ccgccgacca tgtggggcgc cgtcgcgggc ccggcgctgg
     4861 tggcgatcgg cgctctgctg ggttcgaggc ggttgcgccg caccggcgtg ttcctgtgcg
     4921 ccgccaatgt ggcggccatg accgacatcg gcgtg
//
LOCUS       DASUPW010000036         2877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_285680, whole genome shotgun sequence.
ACCESSION   DASUPW010000036 DASUPW010000000
VERSION     DASUPW010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285680"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VFY44_00800"
     CDS             <1..621
                     /locus_tag="VFY44_00800"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX5908996.1"
                     /translation="LTRALARIRDSSTGFGVFALVASVWIGSSFWGALDTAFGEIYHV
                     ESRSWLRQKWFSFGMLAVVLLFMAATVAVPTLQSLLTTSTKDLPLGLSEVDGLIFGVS
                     LAAGLILLFAVLSLIYWAVPNHRMPWRAIWPGALAATAAIGIVDYAFPVYLGSISTIA
                     RFGTTFVFVLIVLLWFYALAMILLSGAIINALRFELHDTGKLETPA"
     gene            653..1282
                     /locus_tag="VFY44_00805"
     CDS             653..1282
                     /locus_tag="VFY44_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5908997.1"
                     /translation="MPSSPSPTSFVIIDEHPVVRHGVRSLIENTWSDAAVTDVSNLAD
                     ALEHTGSNGNGRIVVVDPWRAGADVGELVKRLTTELNAPVVIFTADGGARLLTESLKA
                     GVRGYVRKDSPPEDLVRAIRAAQAGEFYVDPSLSSTIVLEEGDRTLSARQREILQMLA
                     NGLQTEAVARQLGLSTETVRTHTKRILAKLEASTRTQAVAIGLRYGLIQ"
     gene            complement(1288..2292)
                     /locus_tag="VFY44_00810"
     CDS             complement(1288..2292)
                     /locus_tag="VFY44_00810"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5908998.1"
                     /translation="MPEPDRLTITPLRGPLARLDRAREDLAKAWLMRMIERASLDEIR
                     KLPTEQIAAELPAVISDVLQIAATEDRNRLELPEDVRERAVRLADLRQGEEPSAAELT
                     RDVAGIQSVVLEALSRDAEEIGAEEFAAVATAVTEAISGLQVTAVEALVDQRSREAET
                     LANTDPLTGLANMRHLQGQLRHNLGLVKRYEQPFALLVIDVDGLKRVNDGQGHQAGDR
                     VLVQVGLAMRRTIRAVDTPARIGGDEFCVLAPSQTADAARPLAERLAAAVAGETSGDD
                     ADPGVGASVGVVSCPEHGDDAEALLDAAEEAMYRAKAMGEPVALGERAANTQSANGSS
                     "
     gene            2384..>2877
                     /locus_tag="VFY44_00815"
     CDS             2384..>2877
                     /locus_tag="VFY44_00815"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5908999.1"
                     /translation="MPEPMPVETARQIVLDGLRPLPGEDVPLDAARGRVLAEAVTSQA
                     DVPPFASSAMDGFAIPSGASGELTIAGESRAGHPYDGTVEPGTAVRISTGAVVPEGTG
                     AVVQVEVTEERGGVVTVPARENGDNIRGAGEDIRAGTTLLSPGAVLGPAALGVLASIG
                     HTTAR"
BASE COUNT          366 a         1065 c         1020 g          426 t
ORIGIN      
        1 ctgacgcgcg cgctggcccg gatccgggac tcgtcgaccg gcttcggggt gttcgcgctg
       61 gtggccagcg tgtggatcgg ctcctccttc tggggcgccc tggacaccgc cttcggcgag
      121 atctaccacg tggagtcgcg cagctggcta cgccagaagt ggttctcctt cgggatgctg
      181 gccgtggtgc tgctgttcat ggccgccacc gtggccgtgc ccacgctcca gagcctgctg
      241 accaccagca cgaaggacct cccgctgggg ctgtcggagg tcgacggcct gatcttcggc
      301 gtgtcgctgg ccgcgggcct gatcctgctg ttcgcggtcc tgagcctgat ctactgggcg
      361 gtgcccaacc accggatgcc ctggagggcg atctggcccg gggccctggc ggccaccgcg
      421 gcgatcggca tcgtggacta cgcgttcccc gtctacctgg gcagcatcag cacgatcgcc
      481 cgcttcggga ccacgttcgt cttcgtgctg atcgtgctgc tgtggttcta cgcgctggcc
      541 atgatcctgc tgtccggggc catcatcaac gccctccggt tcgagctcca cgacaccggc
      601 aagctggaga cgcccgccta gcctttctac atctgggtat aaggcgtacc gaatgcctag
      661 ctctccctct ccgacctcct tcgtgatcat cgatgagcac ccggtggtcc gccacggggt
      721 gcgctcgctg atcgaaaaca cctggtccga cgccgctgtg accgacgtct cgaacctggc
      781 cgacgcgctc gagcacaccg ggtccaacgg caacggccgc atcgtcgtgg tcgacccgtg
      841 gcgtgccggg gccgacgtcg gcgagctggt caagcgcctg accacggagc tgaacgcccc
      901 ggtcgtgatc ttcaccgccg acggcggcgc ccggctgctg accgagtcgc tcaaggcggg
      961 cgtccgcggg tacgtgcgca aggactcccc gccggaggac ctggtgcgcg ccatccgcgc
     1021 cgcccaggcc ggcgagttct acgtggaccc cagcctgtcg tcgacgatcg tcctcgagga
     1081 gggcgaccgc accctcagcg cgcgccagcg cgagatcctc cagatgctgg ccaacgggct
     1141 ccagaccgag gccgtggccc gccagctcgg cctgtccacg gagacggtgc gcacgcacac
     1201 caagcgcatc ctggccaagc tcgaggcgtc gacgcgcacg caggccgtgg ccatcggcct
     1261 tcggtacggc ctgatccagt agccctctta gctgcttccg ttggcgctct gggtgttcgc
     1321 cgcccgctcg cccagcgcca ccggctcgcc catcgccttg gcgcggtaca tcgcctcctc
     1381 ggcggcgtcc agcagggcct cggcgtcgtc gccgtgctcg gggcacgaga ccacgccgac
     1441 cgatgcgccc acgccggggt cggcgtcgtc gcccgaggtc tcgccggcca cggccgcggc
     1501 caggcgctcg gccagcggcc gcgccgcgtc ggcggtctgg ctgggagcga gcacgcagaa
     1561 ctcgtcgccc ccgatccggg cgggggtgtc caccgcgcgg atggtgcggc gcatggccag
     1621 cccgacctgc accagcaccc ggtcgccggc ctggtggccc tggccgtcgt tgacgcgctt
     1681 gaggccgtcg acgtcgatga ccagcagcgc gaacggctgc tcgtagcgct tgaccagccc
     1741 gaggttgtgg cgcagctggc cctggaggtg gcgcatgttg gccaggccgg tcagcgggtc
     1801 ggtgttggcc agggtctcgg cctcgcgtga gcgctggtcc accagcgcct ccaccgcggt
     1861 gacctgcagg cccgagatgg cctcggtgac cgccgtggcc accgccgcga actcctccgc
     1921 gccgatctcc tcggcgtcgc gggacagcgc ctcgagcacc acggactgga tgccggcgac
     1981 gtcgcgggtc agctcggccg cgctgggctc ctcgccctgg cgcaggtcgg ccaggcgcac
     2041 ggcgcgctcg cgcacgtcct cggggagctc gaggcggttg cggtcctcgg tggcggcgat
     2101 ctggagcacg tcggagatca cggccggcag ctcggcggcg atctgctcgg tgggcagctt
     2161 gcggatctcg tccagcgacg cacgctcgat catccgcatc agccaggcct tggccaggtc
     2221 ctcccgcgca cgatcaagcc gcgccagcgg cccgcgtaac ggcgtaatgg tcagacggtc
     2281 gggctcaggc acggcggctc cgtacggacg atcgaggacg agactacggt gcgctacgga
     2341 gggaaaaacg ggcccgcggc cgcagcctgg gaggatccga ggcatgcccg agcccatgcc
     2401 cgtcgagacc gcgcggcaga tcgtcctgga cggcctgcgc ccgctcccgg gcgaggacgt
     2461 gccgctggac gccgcccgcg ggcgggtcct ggccgaggcc gtgaccagcc aggcggacgt
     2521 cccgcccttc gccagctcgg ccatggacgg gttcgccatc ccctccggcg cctccggcga
     2581 gctgaccatc gcgggcgagt cgcgcgcggg ccatccctac gacgggacgg tcgagcccgg
     2641 caccgcggtg cgcatctcca ccggcgcggt ggtgcccgag ggcaccggcg cggtggtcca
     2701 ggtcgaggtg accgaggagc gcggcggcgt ggtcaccgtc ccggcgcgcg agaacggcga
     2761 caacatccgc ggggcgggcg aggacatccg cgcgggcacc accctgctct cgccgggtgc
     2821 ggtgctgggc cccgccgccc tgggcgtgct ggccagcatc ggccacacca cggcccg
//
LOCUS       DASUPW010000037         5360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1393560, whole genome shotgun sequence.
ACCESSION   DASUPW010000037 DASUPW010000000
VERSION     DASUPW010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393560"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..800)
                     /locus_tag="VFY44_00820"
     CDS             complement(90..800)
                     /locus_tag="VFY44_00820"
                     /inference="COORDINATES: protein motif:HMM:NF014089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HEX5909000.1"
                     /translation="MQSTLGEFQESQSQEAFNLQNSKLLKVRLDAVTIQAKLGSMVAY
                     QGDVKFEHAGSGGMGRMLKKAVSGEGTSLMKMTGTGEVFLADTAQDIHLLKLENDAIT
                     CNGANVLAFDAGIDWDITKVQGAGAMMGGSGLFNMALQGSGWVAIISDGPPVLLNTGD
                     APTFADPQAAITWSSSVSTSIKTDVNLKNFIGRGSGESLQMAFQGQGWVLVQPSEGRI
                     VPTAAGGGGGGLLGQLSG"
     gene            complement(868..1986)
                     /locus_tag="VFY44_00825"
     CDS             complement(868..1986)
                     /locus_tag="VFY44_00825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX5909001.1"
                     /translation="MFEVDDALRLVIATGGSDLHLKVPAHPIIRQQGKLVPIAGTDPL
                     APEDTERIFRGMLTNPKKLEEFEDDNEVDFSYGIPGLARFRVNAFRQRGSISLVCRAI
                     PFDIKTAEELLLPKVINDLADEERGIILLTGTTGSGKSTTLAAMIDRMNTTSAKHIVT
                     IEDPVEFLHSDKQSIINQREVGEDTGSFARALRRVLRQDPDVILIGEMRDEETVRTAL
                     SAAETGHLVLSTVHTVDAAESVNRIIDFFKPEEQKQARAMLAGTLKGVVSQRLVPTPD
                     GKGRVATCEVLRMTGRVRDMIMDPEQTGQLHEVISEGAYYGMQTFDQALLDHVQNGRV
                     TMEDALKAASAPHDFKLLVSSDGQRSTSVDQVYAPSGA"
     gene            complement(2039..2428)
                     /locus_tag="VFY44_00830"
     CDS             complement(2039..2428)
                     /locus_tag="VFY44_00830"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5909002.1"
                     /translation="MSHSIKNLREVHDSAPDHGFGDNQEARFAREALDAETTGLAYHV
                     IKAGKRQGFAHKHDDAEEVYVVLSGTGRIKLDDEILDIGPMDAIRIAPSVARALEAGA
                     DAPMEILAFGPHHDKDGELVHDPGFWG"
     gene            complement(2449..3288)
                     /locus_tag="VFY44_00835"
     CDS             complement(2449..3288)
                     /locus_tag="VFY44_00835"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEX5909003.1"
                     /translation="MGAPRALAVMTVAALACAPAAGATTVRQSAPGPFGNTTLVIQAG
                     PAEANAVTVRTQGFAFVAEDAGAPVTAGPGCVQAATNRAECTVAMPPEVNVDLGDGND
                     GFAIPVPSITFGLQVSVAGGPGSDALSTATQMSSGVTLDGGDGDDLLVGGYPAQEQMS
                     GGPGNDQLRAQNGDGDDVLSCGPGVDAVTADRGDGIAADCENRPVFADTRRCVVPQLR
                     GAKLSTARRRLSRAGCRTGAVRRRYSRRVRKGRVIASAPKAGSRRATGTKVRLSVSRG
                     RRR"
     gene            complement(3309..4409)
                     /locus_tag="VFY44_00840"
     CDS             complement(3309..4409)
                     /locus_tag="VFY44_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5909004.1"
                     /translation="MSVAELQELEEVKVLLTKGQTAGVLTYAEIATALAEVDLDEGDI
                     EDLHTHLEKSEIELVEEIDPAQAASKDVERAPDKRGRRKKATIDLKPDMTTDSLQLFL
                     KDIGKVRLLTAQEEVILAKAIERGDLDAKQKMVESNLRLVVSIAKNYRNQGLPFLDLI
                     QEGTLGLVRAAEKFDYRKGFKFSTYATWWIRQAIARALADKARTIRIPVHVVEKLNKI
                     GRAERKLVTELGREPTPEEIADVTGIEPDEVDSIKRSAQAPISLEKPVGDEEESEFGQ
                     FIADERAESPFDRAAEILTKESLREALENLSYRERRVLELRYGLGGEHPRTLDEVGRT
                     FNVTRERIRQIENQSLKKLQSLAEAQKLREVA"
     gene            4600..4965
                     /locus_tag="VFY44_00845"
     CDS             4600..4965
                     /locus_tag="VFY44_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909005.1"
                     /translation="MTPEEPGLLGNLPRSRPGTRSDKRGDGTGSKPAPKRSQAAKPGV
                     KNAAAAKKKAPAAKKNAPKKPPAPKSQPAPEPRPEPVASKSVPPSPSPLESAVKVAGA
                     GLKVAEGVTREVLRRLPRP"
     gene            4974..>5360
                     /locus_tag="VFY44_00850"
     CDS             4974..>5360
                     /locus_tag="VFY44_00850"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEX5909006.1"
                     /translation="MPAPAGQEHRPPLRAFKDFWVKAYRDNVTGLAGMVAYNLLLSVF
                     PLALLALFIAGRVLESPDLEASVLSDLQQLFPNATDATLTRALARIRDSSTGFGVFAL
                     VASVWIGSSFWGALDTAFGEIYHVESR"
BASE COUNT          714 a         1877 c         1872 g          897 t
ORIGIN      
        1 cgctgaagcc atgcgtttac cagtctagcg cgcatgactt caggaaatga agcggggccc
       61 gggcggtcac ccgggccccg ctggaacgcc tagcccgaga gctggccgag caggccgccg
      121 ccaccgccgc cggcggcggt cggcacgatc cggccctcgc tgggctgcac cagcacccag
      181 ccctggccct ggaacgccat ctgcagcgac tcgcccgagc cgcggccgat gaagttcttc
      241 aggttcacgt cggtcttgat cgaggtgctg acgctgctcg accacgtgat cgcggcctgc
      301 gggtcggcga acgtgggcgc gtcgcccgtg ttcagcagga cgggcgggcc gtcggagatg
      361 atggccaccc agccgctgcc ctggagggcc atgttgaaca ggccgctccc gcccatcatg
      421 gcgccggcgc cctggacctt ggtgatgtcc cagtcgatgc cggcgtcgaa ggccagcacg
      481 ttggcgccgt tacaggtgat ggcgtcgttc tccagcttga gcaggtggat gtcctgcgcg
      541 gtgtcggcca ggaacacctc gccggtgccg gtcatcttca tcagcgacgt gccctcgccg
      601 gacacggcct tcttgagcat gcgccccatg ccgcccgagc cggcgtgctc gaacttgacg
      661 tcgccctggt aggccaccat cgagccgagc ttggcctgga tggtcaccgc gtcgaggcgg
      721 accttcagca gcttgctgtt ctggaggttg aaggcctcct gggactggct ttcctggaat
      781 tcgccgaggg tgctctgcac cgctcctcct tcttggggtt caggtcgaga tgcgctgtgc
      841 gcactcttga gggtcccccg ggatacgtca agcgccggag ggcgcgtaca cctggtccac
      901 cgaggtggag cgctgcccgt cgctggagac cagcagcttg aagtcgtgcg gggcggacgc
      961 ggccttgagc gcgtcctcca tggtcacccg gccgttctgg acgtgatcga gcagcgcctg
     1021 gtcgaaggtc tgcatgccgt agtaggcgcc ctcgctgatg acctcgtgca gctggcccgt
     1081 ctgctcgggg tccatgatca tgtcccggac gcggccggtc atgcgcagca cctcgcaggt
     1141 ggccacgcgg cccttgccgt ccggcgtggg caccaggcgc tgggacacca cgcccttgag
     1201 cgtgcccgcc agcatggccc gggcctgctt ctgctcctcg ggcttgaaga agtcgatgat
     1261 ccggttcacg gactcggccg cgtcgacggt gtgcaccgtc gagagcacca ggtggccggt
     1321 ctcggcggcc gagagcgcgg tccggacggt ctcctcgtcg cgcatctcgc cgatcaggat
     1381 cacgtccggg tcctggcgca ggacgcgccg cagggcacgg gcgaacgacc cggtgtcctc
     1441 accgacctca cgctggttga tgatcgactg cttgtcgctg tgcaggaact cgaccgggtc
     1501 ctcgatggtc acgatgtgct tggccgaggt cgtgttcatc cggtcgatca tggccgcgag
     1561 cgtggtggac ttgcccgagc cggtcgtgcc ggtcagcagg atgatcccgc gctcctcgtc
     1621 ggccaggtcg tttatgacct tgggcagcag gagctcctcg gcggtcttga tgtcgaacgg
     1681 gatcgcgcgg cagaccagcg agatcgagcc gcgctggcgg aacgcgttca cccggaagcg
     1741 ggccaggccg gggatgccgt aggagaagtc gacctcgttg tcgtcctcga actcctcgag
     1801 cttcttcggg ttggtcagca tcccgcggaa gatgcgctcg gtgtcctcgg gcgccagcgg
     1861 gtccgtgccg gcgatgggga cgagcttgcc ctgctggcgg atgatcgggt gcgcgggcac
     1921 cttcaggtgc agatccgagc ccccggtcgc gatgaccagg cgcagcgcgt cgtccacttc
     1981 gaacatatgg gtctagtcgg ccgcccgcgg cccgcgcttt agccgccggc gggccggctc
     2041 aaccccagaa gcccgggtcg tggaccagct ccccgtcctt gtcgtggtgc gggccgaagg
     2101 ccaggatctc cattggcgcg tcggcaccgg cctccagggc ccgcgccacg gacggcgcga
     2161 tgcggatggc gtccatcggc ccgatgtcca ggatctcgtc gtcgagcttg atccgtccgg
     2221 tgcccgagag caccacgtag acctcctcgg cgtcgtcgtg cttgtgcgcg aacccctggc
     2281 gctttccggc cttgatcacg tggtacgcca gcccggtggt ctcggcgtcc agcgcctcac
     2341 gggcgaaccg ggcctcctgg ttgtcaccga acccgtggtc cggcgcggag tcgtggacct
     2401 cgcggaggtt cttgatcgag tggctcatgg cgcgagccta acccggcctc agcggcgccg
     2461 gcctcggctg accgacagcc gcaccttggt gcccgtggcc cgccggcttc cggccttcgg
     2521 cgcgctggcg atcacgcgcc ccttgcgcac ccgtcgtgag taccggcggc gcaccgcgcc
     2581 ggtgcgacag ccggcgcggc tgagccggcg gcgcgccgtc gacagcttcg ctcccctcag
     2641 ctggggcacg acacaccggc gtgtgtcggc gaacaccggc cggttctcgc agtcagccgc
     2701 gatgccgtcg ccgcggtcgg cggtcaccgc atccacgccc ggcccgcagc tcagcacgtc
     2761 gtccccgtcc ccgttctggg cccgcagctg gtcgttgccg ggcccgcccg acatctgctc
     2821 ctgggccggg tagccaccca ccagcaggtc gtccccgtcc ccgccgtcca gggtgacgcc
     2881 cgatgacatc tgcgtggcgg tggacagggc gtccgacccg gggccgccgg ccacggacac
     2941 ctgcaggccg aaggtgatcg aaggcaccgg gatggcgaag ccatcgttgc cgtcgcccag
     3001 atcgacgttg acttcgggcg gcatggccac cgtgcactcg gcgcggttgg tcgcggcctg
     3061 cacgcagccc gggcccgccg tgaccggagc cccggcgtcc tcggccacga acgcgaaccc
     3121 ctgggtgcgg acggtcaccg cgttggcctc ggcgggtccc gcctggatca ccagtgtggt
     3181 gttgccgaac gggccgggcg cgctctggcg cacggtcgta gccccggccg caggcgcaca
     3241 ggccagcgcg gccacggtca tcacggccag tgctctcgga gcccccacgg gggccaggct
     3301 aggtcaggtc aggcgacttc gcgaagcttc tgcgcctcgg cgaggctctg cagcttcttg
     3361 agcgactggt tctcgatctg gcggatgcgc tcgcgcgtga cgttgaacgt gcggcccacc
     3421 tcgtccagcg tgcgggggtg ctccccgccg aggccgtagc gcagctcgag cacgcgccgc
     3481 tcgcggtagc tcaggttctc cagcgcctcg cgcagcgact ccttggtcag gatctcggcg
     3541 gcgcggtcga acggcgactc ggcccgctcg tcggcgatga actggccgaa ctcggactcc
     3601 tcctcgtcgc cgaccggctt ctcgaggctg atcggggcct gggcggaacg cttgatcgag
     3661 tcgacctcgt cgggctcgat gcccgtgacg tcggcgatct cctccggcgt gggctcgcgg
     3721 cccagctcgg tgaccagctt gcgctcggcg cggccgatct tgttcagctt ctcgaccacg
     3781 tgcaccggga tgcggatggt gcgggccttg tcggccaggg cgcgcgcgat ggcctggcgg
     3841 atccaccagg tcgcgtacgt ggagaacttg aagcccttgc ggtagtcgaa cttctccgcg
     3901 gcgcggacca ggccgagggt gccctcctgg atcaggtcca ggaagggaag gccctggttg
     3961 cggtagttct tggcgatcga gacgaccagg cgcaggttgg actcgaccat cttctgcttc
     4021 gcgtcgaggt caccgcgctc gatggccttg gccaggatga cctcttcctg tgcggtcagc
     4081 aggcggacct tgccgatgtc cttgaggaac agctggaggc tgtccgtggt catgtccggc
     4141 ttgaggtcga tggtcgcctt cttgcggcgg ccgcgcttgt ccggcgcgcg ctcgacgtcc
     4201 ttggaggcgg cctgggcggg gtcgatctcc tcgaccagct cgatctccga cttctccagg
     4261 tgcgtgtgga ggtcctcgat gtcgccctcg tcgaggtcga cctccgccag ggcggtggcg
     4321 atctcggcgt aggtgagcac gccggcggtc tgccccttgg tcaggaggac cttgacctct
     4381 tcgagctctt gcagttcggc tacagacata cggaatccga ttcgttacag ggcctgggca
     4441 cgtgcctcag ccattcaaaa ggccgggcca agcatagccc gcgttggcgt ccgccgacac
     4501 gctgaattcg caattcgcgt gatcgaacga gcagccgagt gaccctttct acccacttgg
     4561 ggggaattcg atacgcggcc gaggctagta tccgcaccga tgacccccga ggagccaggc
     4621 cttctgggca acctgccccg ctcccgcccg ggcacgcgca gcgacaagcg cggcgatgga
     4681 accgggtcca agcccgcgcc gaagcgctcg caggccgcca agccgggcgt caagaacgcc
     4741 gccgcggcga agaagaaggc gccggccgcc aagaagaacg cgcccaagaa gccccccgcg
     4801 ccgaagtcgc agccggcccc cgagccgcgg ccggagcccg tggccagcaa gtccgtgccg
     4861 ccgtccccga gcccgctgga gtccgcggtc aaggtggcgg gcgcgggcct gaaggtggcc
     4921 gagggcgtta cgcgcgaggt gctgcggcgc ctcccgcgtc cgtaggcacg accgtgccgg
     4981 cgcccgcggg gcaggagcac cggccgcccc tgcgggcgtt caaggacttc tgggtcaagg
     5041 cctaccggga caacgtcacc ggcctggccg ggatggtggc ctacaacctg ctgttgtcgg
     5101 tgttcccgct ggccctgctg gcgctgttca tcgccgggcg cgttctggag agccccgacc
     5161 tggaggccag cgtgctgtcc gacctccagc agctgttccc caacgccacc gacgccaccc
     5221 tgacgcgcgc gctggcccgg atccgggact cgtcgaccgg cttcggggtg ttcgcgctgg
     5281 tggccagcgt gtggatcggc tcctccttct ggggcgccct ggacaccgcc ttcggcgaga
     5341 tctaccacgt ggagtcgcgc
//
LOCUS       DASUPW010000038         2963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1117213, whole genome shotgun sequence.
ACCESSION   DASUPW010000038 DASUPW010000000
VERSION     DASUPW010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117213"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VFY44_00855"
     CDS             <1..540
                     /locus_tag="VFY44_00855"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX5909007.1"
                     /translation="NLIRANKGPGGGIFVAHTADQGISVSLSNAIAMMLETGAATLEE
                     LLDARLILEAPLAGLAAYNADDEAKARLRAAVEAEAEADQDDPESLGNADAEIHRTVA
                     AASGNRMLQALVGWVFEVLQPQLVAKVHGTVVHSAVVDQHRALLRAVERGDSARAERA
                     MKDHLLYLRDVLRMAEERG"
     gene            537..1208
                     /gene="pcaH"
                     /locus_tag="VFY44_00860"
     CDS             537..1208
                     /gene="pcaH"
                     /locus_tag="VFY44_00860"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HEX5909008.1"
                     /translation="MSTDPPLLFPDYRATRTRAPKRPLVPVEHTLSELTGPAYGHERP
                     GPDDHDLTRQFDGEPLGERIVLHGRVLDGDGRPVPDTLVELWQANSAGRYRHVVDRHP
                     APLDPNFAGAGRAVTDGDGRYRFVTIKPGAYPWRNHDNAWRAAHIHLSLFGLAFTQRL
                     VTQMYFPGDPLFFQDPIFNSVPDQRGRELMISRFDLGETVPEWALAYEFDIVLRGRDA
                     TPFED"
     gene            1211..1732
                     /gene="pcaG"
                     /locus_tag="VFY44_00865"
     CDS             1211..1732
                     /gene="pcaG"
                     /locus_tag="VFY44_00865"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HEX5909009.1"
                     /translation="MLPTPSQTVGPFFNIGMPGDRTELVAEGTEGAVRIEGVVCDAEG
                     PVVDALLEIWQAGPDGRYDEPGFAGFGRGETDPEDGTYAFVTVKPGQVPGPNGALQAP
                     HLLVSVFARGLLKRLVTRIYFEDEGDANATDPVLAAVDPDRRDTLIARRDGEAYRFDV
                     RLQGDGETVFFDV"
     gene            1722..>2963
                     /locus_tag="VFY44_00870"
     CDS             1722..>2963
                     /locus_tag="VFY44_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX5909010.1"
                     /translation="MTSDDLFGPLFVPERMRQAVSGRAWVEAMLAFEAVLASAEAEAG
                     LIPAQAAEAIAAAAAMPDRFDVAALGQAGRAGGNPAAPLVRALTAAVEGDARAHVHLG
                     ATSQDVMDTASSLVSRQAVGVIDEQLDDACRYARRLIDAYRDTPMVARTLGQQALPTT
                     FGLRAAGWLDALLDARDELAAVPFAAQLGGAAGTMASLGDQGPRVLALLATGLDLDAP
                     SLPWHTARGRIGRLAGALAVTAGTLEKIATDVVGLAQTEVGEVAEGGHGGGSSTRPHK
                     SNPAGSAVAIACAHRVRGEAAILFGTMAQQHERSAGAWQAEWQAVSGALAATGGAAAA
                     VRGVLRELEVRPGRMRANLDLTGGLVMTEAVVTALVGAGAERQAAHDAVAEIAASGQD
                     LRQGVLADGRLSEAQVDAALDP"
BASE COUNT          383 a         1071 c         1137 g          372 t
ORIGIN      
        1 aacctgatcc gggccaacaa gggccccggt ggcggcatct tcgtcgccca caccgccgac
       61 cagggcatca gcgtcagcct cagcaacgcc atcgcgatga tgctggagac cggggccgcc
      121 acgctggagg agctgctgga cgcccgcctg atcctggagg cgccgctggc cgggctggcc
      181 gcctacaacg ccgacgacga ggccaaggca cggctgcgcg ccgcggtcga ggcggaggcc
      241 gaggccgacc aggacgaccc cgagtcgctg ggcaacgccg atgccgagat ccaccgcacg
      301 gtggccgccg catcgggcaa ccggatgctc caggcgctgg tggggtgggt gttcgaggtg
      361 ctccagcccc agctcgtggc caaggtgcac gggaccgtgg tgcactccgc cgtggtcgac
      421 cagcaccgcg cgctgctgcg cgcggtggag cgcggcgact ccgcccgcgc cgagcgggcc
      481 atgaaggacc acctgttgta tctgcgcgac gtgctgcgca tggccgagga gagaggatga
      541 gcaccgaccc gccgctgctg ttcccggact accgcgccac ccggacgcgc gcgccgaagc
      601 gcccgctggt cccggtggag cacacgttgt ccgagctgac cgggcccgcg tacggccacg
      661 agcgcccggg tcccgacgac cacgacctga cccggcagtt cgacggcgag ccgctgggcg
      721 agcggatcgt gctccacggc cgcgtcctgg acggtgacgg ccgcccggtc ccggacacgc
      781 tcgtcgagct gtggcaggcc aactccgcgg ggcgctatcg gcacgtggtc gatcgccacc
      841 cggcgccgct ggaccccaac ttcgccggcg cgggccgggc ggtcaccgac ggcgacgggc
      901 gctaccgctt cgtcaccatc aagccggggg cgtacccgtg gcgcaaccac gacaacgcgt
      961 ggcgcgcggc ccacatccac ctctcgctct tcgggctggc gttcacccag cggctggtga
     1021 cacagatgta cttccccggc gacccgctct tcttccagga cccgatcttc aactcggtgc
     1081 ccgaccagcg gggacgcgag ctgatgatct cccgcttcga cctgggcgag accgtgcccg
     1141 agtgggcgct ggcgtacgag ttcgacatcg tcctgcgtgg ccgtgacgcc acgccgttcg
     1201 aggactgagc gtgctgccca cgccctccca gacggtcggc ccgttcttca acatcggcat
     1261 gcccggcgac cggaccgagc tggtggccga gggcaccgag ggcgccgtgc ggatcgaagg
     1321 cgtggtctgc gatgccgagg ggccggtggt cgatgcgctg ctcgagatct ggcaggcggg
     1381 ccccgatggg cgctacgacg agcccggctt cgccgggttc ggccgcgggg agaccgaccc
     1441 cgaggacggc acgtatgcct tcgtcacggt caagcccggg caggttcccg ggccgaacgg
     1501 agcgctccag gccccgcacc tcctggtgtc cgtgttcgcc cgggggctgc tcaagcggct
     1561 ggtcacgcgc atctacttcg aggacgaggg cgacgccaac gccaccgatc ccgtgctggc
     1621 cgccgtcgac cccgaccgcc gcgacaccct gatcgcacgg cgcgacggcg aggcgtaccg
     1681 tttcgacgtg cgcttgcagg gcgacggcga gacagtgttc tttgacgtct gacgatctct
     1741 tcggccccct gttcgtgccc gagcgcatgc gccaggccgt gtccgggcgc gcgtgggtgg
     1801 aggcgatgct ggcgttcgag gccgtgctgg cgtcggccga ggccgaggcg ggcctgatcc
     1861 cggcccaggc ggccgaggcg atcgcggctg cggcggcgat gccggaccgc ttcgacgtcg
     1921 ccgcgctggg ccaggcgggc cgtgccgggg ggaacccggc ggccccactg gtgcgggcgc
     1981 tgacggccgc ggtcgagggc gacgcacggg cccacgtgca cctcggcgcc acgagccagg
     2041 acgtcatgga cacggcgagc tccctggtct cgcgccaggc ggtgggcgtg atcgacgagc
     2101 agctggacga cgcctgccgg tacgcgcggc gcctgatcga cgcgtaccgc gacacgccga
     2161 tggtggcgcg cacgctgggt cagcaggcgc tgcccaccac cttcggcctc cgcgccgccg
     2221 ggtggctgga cgcgctgctg gacgcccgcg acgagctggc cgcggtgccg ttcgcggcgc
     2281 agctgggcgg cgccgcgggg accatggcgt cgctgggcga ccaggggccg agggtgctgg
     2341 ccctgctggc caccgggctg gacctggacg cgccgtcgct gccgtggcac accgcgcgcg
     2401 gccgcatcgg ccgcctggcg ggcgcgctgg cggtcacggc cggaacgctg gagaagatcg
     2461 ccaccgacgt cgtgggcctg gcgcagaccg aggtgggcga ggtggccgag ggcggccacg
     2521 ggggcggctc ctccacgcgt ccccacaaga gcaaccccgc gggctcggcg gtcgcgatcg
     2581 cctgtgcgca ccgcgtgcgg ggcgaggccg cgatcctgtt cggcacgatg gcgcagcagc
     2641 acgagcgctc cgccggcgcg tggcaggcgg agtggcaggc ggtcagcggc gcgctggcgg
     2701 ccaccggtgg cgcggcggcc gcggtccggg gcgtcctgcg cgagctggag gtccggccgg
     2761 ggcgcatgcg ggccaacctc gacctgaccg ggggccttgt catgaccgag gcggtggtca
     2821 cggcgctggt cggcgccggc gccgagcgtc aggccgcgca cgatgcggtc gcggagatcg
     2881 cggcgagcgg acaggatctg cgccaggggg tcctggccga cgggcggctg tccgaggccc
     2941 aggtggacgc cgcgctggac ccc
//
LOCUS       DASUPW010000039         6286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1948917, whole genome shotgun sequence.
ACCESSION   DASUPW010000039 DASUPW010000000
VERSION     DASUPW010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1948917"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VFY44_00875"
     CDS             <1..477
                     /locus_tag="VFY44_00875"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX5909011.1"
                     /translation="DWPDDRVWEEASTRMALDGEPFALQTGEVFEKGVTPMRSYVCEP
                     MQHGRLYLAGDAVHIVPPTGAKGLNLAVADVRVLAEALTAWLADGREDLLDAYSQTCL
                     RRVWRTQWFSYWMTSMLHRMPDDADGFQSRLQRAQLDYVCSSEAASRSLAENYVGF"
     gene            complement(474..1595)
                     /locus_tag="VFY44_00880"
     CDS             complement(474..1595)
                     /locus_tag="VFY44_00880"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005085.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEX5909012.1"
                     /translation="MAHRDKPPFRADHVGSLLRPEALLKARDDHAAGTIDDDALKAAE
                     DEAIREVVAMQEEVGLQSATDGEFRRQSWHMDFIYQLGGVEQVEGETLHVQFKSDDGE
                     TEFSPPAMKVTGKVELEHTIFGDAFAFLRDTVTTNTPKLTIPSPSMVHYRGGNSSIDQ
                     SAYPDVDRFWDDLTAAYAAEAKAVAELGCTYLQLDDTSLAYINDPAQRRHIEEIGGDP
                     DHLHEQYIANINRALADRPDGLSITTHLCRGNMQSMWAAEGGYDFVAEALFNDLDVDG
                     FFLEYDDERSGTFEPLRFVPKGKQVVLGLVTTKRPELEDKDLLKRRIEEASQYVDVDQ
                     LCLSGQCGFSSTVEGNRLTRDDQRAKLALIVETADEVWG"
     gene            complement(1656..2816)
                     /locus_tag="VFY44_00885"
     CDS             complement(1656..2816)
                     /locus_tag="VFY44_00885"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5909013.1"
                     /translation="MTSELQPTTDRSTRALVTGVALIVATVLPGFLTASLATRIRDDF
                     SFENSALGLCVAVFYVVSAAASNPAGRLLERIGPVRGMRLAAALTAVSCLTVVLAANS
                     TATLTAALVLAGVANGLGSPTVSALFKRLIPEARQGMAFGAQQAGAPLGSLLAGLALP
                     LIAIPFGWRWAYAAAGLMGLVAVALAPRGVAAPAPGSRAKPPRGFTAIHGIAIAAALA
                     SAAGVGFVSFLVSYSVENDISEAAAGLLLGGVSLASAMSRIVLGSVLDRSRADALMPL
                     ALMLAVSVGGYLLLMADQPALIVVAALVAGSLGWAWPGGLNLAVVQRSPDAPAWAVGV
                     MMTGLFAGAVAGPLLVGVLADHGHFDSAWLLCGAFALGAAATLAVTRRRERA"
     gene            2908..3867
                     /locus_tag="VFY44_00890"
     CDS             2908..3867
                     /locus_tag="VFY44_00890"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5909014.1"
                     /translation="MAVGLLAMFAAVGAGCGDGGGGGGTGGNEPATLKVGVIPIADVA
                     PLYVGIDQGFFKDEKLTIKPQLAEGGAAITPAVVSGDNQIGFSNNTSLLIAASKKLPI
                     QIITQGVLAGKTEKEAWAELMVPKGSPIKTPKDLEGKSIAVNTLKNVCELTIKASLEK
                     KGVDVDKLKFSEIPFPDMNGALEAKRVDAACEVEPFVSQAKAAGSTGIDPFYVNTAPD
                     LSVATYFTSTQYAKQNPEVLKRFAKAMNKSLDYTQQNPDAARKVLAEYTEIPPDAIAK
                     IRLPKWSSKLDPATIELQAELAEKYGLVDSKPDVEELIQEPGS"
     gene            3864..4673
                     /locus_tag="VFY44_00895"
     CDS             3864..4673
                     /locus_tag="VFY44_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5909015.1"
                     /translation="MTALSHTGGRARPRLPDAALPWLAVLGALALFEVAPRVGILPSD
                     HFPPISDSIRTLGEQVSEGSFWTAVGQTMQGWALGLGIAAALGVPLGIVIGSNRLLYR
                     ACRGLIEFLRPIPSVALIPLAILIYGTGMQSKVFLAAFASFWQVIVATLYGVQDVDPV
                     ATDTARSFGFSRFKRLFRVTLPSAVPYIATGLRIASAVSLILTVTAELVIGAPGLGLA
                     INVARSGGNEELMYALILTTGILGLLLNMVFVKVEKRVLHWHPSHREGVPS"
     gene            4670..5455
                     /locus_tag="VFY44_00900"
     CDS             4670..5455
                     /locus_tag="VFY44_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5909016.1"
                     /translation="MSDPAPRRWTGAALEVAVPLIALALWAVLSSNSQTYYFPPLTDI
                     LTTFKDTWLFDRVGSDVVPSLARMFSGFLIAVVVAVTAGLALGASPRARRAFAPIVEF
                     LRAIPPPALLPFAILVIGVGNSMKVFIIAFVCLWPVLLNTIDGVTGVDPTLRETARSY
                     GIPRSQVVRRVMLPAATPQIFAGMRTSLSLALILMVISEMVASTNGIGYFVLQSQRTF
                     AIPEMWSGIILLGLLGYLFNGVFVLIEKRVLRWHRGARASALS"
     gene            5464..6228
                     /locus_tag="VFY44_00905"
     CDS             5464..6228
                     /locus_tag="VFY44_00905"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5909017.1"
                     /translation="MLKIDHLGKTYGSGDKAVKAIADLDFEVNEKELVCVVGPSGCGK
                     TTLLKCMSGLLPATTGQVLLHGQPIEGPPENMALVFQEYSRSLFPWMTVRQNVSFPLS
                     RRKDTEGPVEQAVESVGLSDFLDRYPWELSGGMQQRVAIARALAYQPEILLMDEPFAS
                     VDAQTRADLEDLVLQVREQYGVTIVFVTHDIDESVYLGDRIVVLTPSPTEVQEQLDVD
                     LPRPRDQIDTKELPEFAQLRGHVYRSIKRKEKPAVA"
BASE COUNT          937 a         2214 c         2200 g          935 t
ORIGIN      
        1 gactggcccg acgaccgcgt ctgggaggag gcgtccacgc gcatggctct ggacggcgag
       61 ccgttcgccc tccagaccgg ggaggtgttc gagaagggcg tcacgcccat gcgcagctac
      121 gtctgcgagc cgatgcagca cggccggctg tacctggccg gcgacgccgt gcacatcgtg
      181 ccgcccaccg gtgcgaaggg cctgaacctg gcggtggccg acgtgcgcgt gctggccgag
      241 gcgctgaccg cctggctggc cgacggccgc gaggacctgc tggacgccta ttcgcagacg
      301 tgcctgcggc gcgtgtggcg cacgcagtgg ttctcctact ggatgacgtc gatgctgcac
      361 cggatgcccg acgacgccga cggcttccag tcgcggctcc agcgcgcgca gctggactac
      421 gtgtgctcct cggaggccgc ctcgcggtcg ctggccgaga actacgtggg cttctagccc
      481 cacacctcgt ccgccgtctc cacgatcagg gccagcttgg cgcgctggtc gtcccgcgtc
      541 agccggttgc cctccacggt cgaggagaag ccgcactgcc ccgacaggca cagctgatcg
      601 acgtcgacgt actgcgaggc ctcctcgatc cggcgcttga gcaggtcctt gtcctccagc
      661 tcggggcgct tggtggtcac cagccccagc accacctgct tgcccttggg cacgaaccgc
      721 agcggctcga acgtgcccga gcgctcgtcg tcgtactcca ggaagaagcc gtccacgtcg
      781 aggtcgttga acagcgcctc ggccacgaag tcgtagccgc cctcggccgc ccacatggac
      841 tgcatgttgc cgcggcacag gtgggtggtg atcgacagcc cgtcgggccg gtcggccagc
      901 gcgcggttga tgttggcgat gtactgctcg tgcaggtggt cggggtcgcc gccgatctcc
      961 tcgatgtgcc ggcgctgggc cgggtcgttg atgtaggcca ggctggtgtc gtccagctgg
     1021 aggtaggtgc agcccagctc ggccacggcc ttcgcctccg cggcgtaggc ggcggtcagg
     1081 tcgtcccaga accggtccac gtcggggtag gccgactggt cgatcgagga gttgccaccg
     1141 cggtagtgga ccatgctggg ggaggggatc gtcagcttgg gcgtgttggt ggtgaccgtg
     1201 tcgcgcagga acgcgaacgc gtccccgaag atggtgtgct ccagctcgac cttgccggtg
     1261 accttcatcg ccggcgggga gaactcggtc tcgccgtcgt cgctcttgaa ctgcacgtgc
     1321 agggtctcgc cctccacctg ctcgacgccg ccgagctggt agatgaagtc catgtgccac
     1381 gactggcggc ggaactcgcc gtcggtcgcg ctctgcaggc cgacctcctc ctgcatggcc
     1441 acgacctcgc ggatggcctc gtcctcggcg gccttcagcg cgtcgtcgtc gatcgtcccg
     1501 gccgcgtggt cgtcgcgcgc cttcagcagc gcctcgggcc gcagcaggct tccgacgtgg
     1561 tccgcccgga acgggggctt gtccctgtgt gccatgtcct cctcctcggt cgtctctgct
     1621 cccgggtacc cgccggccgc gaccctcact cggctttagg cgcgctcgcg ccgccgcgtc
     1681 acggccagcg tggcggcggc ccccagggcg aaggccccgc acagcagcca ggcgctgtcg
     1741 aagtgcccgt ggtcggccag cacccccacc agcagcggcc cggccaccgc gccggcgaac
     1801 agcccggtca tcatcacccc gacggcccag gcgggggcat ccgggctgcg ctgcaccacg
     1861 gccaggttca ggccgccggg ccaggcccag cccagcgatc ccgccaccag cgcggccacg
     1921 acgatcaacg ccggctggtc ggccatcagc agcaggtagc cgcccacgct gaccgcgagc
     1981 atcagcgcca gcggcatcag cgcgtccgcg cggctgcggt cgagaaccga gccgagcacg
     2041 atccgcgaca tggccgacgc caggctgacg ccgcccagca acaggccggc ggcggcctcg
     2101 gagatgtcgt tctcgaccga gtaggagacc aggaaggaca cgaagcccac gccggccgcg
     2161 ctggccagcg cggcggcgat ggcgatgccg tggatagcgg tgaacccgcg cggcggcttc
     2221 gcgcgcgagc cgggcgccgg ggcggcgacg ccccgcggcg cgagcgcgac ggccaccagc
     2281 cccatcagcc ctgccgccgc gtacgcccac cgccagccga acgggatcgc gatcagcggc
     2341 agggccagcc cggccagcag cgaccccagg ggcgcgcccg cctgctgggc cccgaaggcc
     2401 atgccctggc gggcctcggg gatcaggcgc ttgaacaggg cggacacggt ggggctgccc
     2461 aggccgttgg ccacgccggc caggaccagg gccgcggtca gcgtggcggt cgagttggcc
     2521 gccaggacca cggtcaggca ggacacggcg gtcagcgcgg ccgcaaggcg catgccccgg
     2581 acgggcccga tgcgttccag cagccggccg gcggggttcg acgcggccgc cgacacgacg
     2641 tagaacaccg ccacgcacag gcccagcgcc gagttctcga acgagaagtc gtcgcggatc
     2701 cgcgtggcca ggctggcggt caggaacccg ggcagcaccg tggccacgat cagcgccacc
     2761 ccggtgacca gggcgcgggt gctgcggtcg gtggtcggct ggagttcgga ggtcacgtga
     2821 aataaaaagt ctggtctatg ggtacacctg cccccgaggg cggatttcgt accgagcgag
     2881 ggagaggcaa tgcgcaagtt gagggtcatg gcggtcggac tgctggcgat gttcgcggcg
     2941 gtcggcgccg gttgcggcga cggcggcggc gggggtggca ccggcgggaa cgagcccgcg
     3001 acgctgaagg tgggggtcat cccgatcgcg gacgtcgcgc ctctctacgt gggaatcgac
     3061 cagggcttct tcaaggacga gaagctcacg atcaagcccc agctggccga gggcggcgcg
     3121 gccatcacgc ccgcggtggt cagcggcgac aaccagatcg gcttctcgaa caacacctcg
     3181 ctgctgatcg cggcctcgaa gaagctgccg atccagatca tcacccaggg cgtcctggcc
     3241 gggaagaccg agaaggaggc ctgggccgag ctgatggtgc ccaagggcag cccgatcaag
     3301 acgcccaagg acctcgaggg caagagcatc gccgtgaaca cgctcaagaa cgtctgcgag
     3361 ctgaccatca aggcctcgct cgagaagaag ggcgtggacg tcgacaagct caagttctcg
     3421 gagatcccct tccccgacat gaacggcgcg ctggaggcca agcgcgtgga cgccgcgtgt
     3481 gaggtcgagc ccttcgtgag ccaggccaag gccgccggct ccacggggat cgaccccttc
     3541 tacgtgaaca cggcgcccga cctgagcgtg gccacctact tcacctcgac gcagtacgcc
     3601 aagcagaacc ccgaggtgct gaagcggttc gccaaggcca tgaacaagtc gctggactac
     3661 acgcagcaga accccgacgc cgcgcgcaag gtcctggccg agtacaccga gatcccgccg
     3721 gatgcgatcg cgaagatccg gctgcccaag tggagctcca agctggaccc ggcgaccatc
     3781 gagctccagg cggagctggc cgagaagtac ggcctggtcg actccaagcc cgacgtcgaa
     3841 gagttgatcc aagagcctgg ttcgtgaccg cgctgagcca caccggcggg agggcccggc
     3901 cgcggctgcc ggacgcggcc ctgccgtggc tggcggtcct gggcgcgctg gcgctgttcg
     3961 aggtcgcgcc gcgcgtcggg atcctgccct ccgaccactt cccgcccatc agcgactcca
     4021 tccggacgct gggcgagcag gtctccgagg gcagcttctg gaccgccgtc gggcagacca
     4081 tgcaggggtg ggcgctgggc ctgggcatcg ccgcggcgct gggcgtgccg ctgggcatcg
     4141 tcatcggctc caaccggctg ctctaccgcg cctgccgcgg gctgatcgag ttcctgcgcc
     4201 ccatcccctc ggtggcgctg atcccgctgg ccatcctgat ctacggcacg ggcatgcaga
     4261 gcaaggtgtt cctggccgcg ttcgcctcgt tctggcaggt catcgtcgcg acgctgtacg
     4321 gggtccagga cgtggacccg gtggccaccg acaccgcccg ctcgttcggc ttctcgcgct
     4381 tcaagcgcct gttccgggtg accctgccga gcgcggtgcc ctacatcgcc accggcctgc
     4441 ggatcgcgtc ggcggtgtcg ctgatcctga cggtcaccgc cgagctggtc atcggcgctc
     4501 ccggcctggg tctcgcgatc aacgtcgcgc ggtcgggcgg gaacgaggag ctgatgtacg
     4561 cgctgatcct gaccaccggc atcctcggcc tgctgctgaa catggtgttc gtgaaggtcg
     4621 agaagcgggt gctgcactgg catccgtcgc acagggaggg ggtgccgtcg tgagcgaccc
     4681 ggcgccccgc aggtggaccg gcgcagccct cgaggtcgcg gtgccgctga tcgccctcgc
     4741 gctgtgggcc gtgctgtcca gcaacagcca gacctactac ttcccgccgc tcaccgacat
     4801 cctcaccacc ttcaaggaca cgtggctgtt cgaccgcgtg ggcagcgacg tcgtgcccag
     4861 cctggcgcgc atgttctcgg gcttcctgat cgccgtcgtg gtggccgtga ccgcgggcct
     4921 cgcgctgggc gcctcgccgc gggcgcgacg ggcgttcgcg cccatcgtcg agttcctgcg
     4981 ggccatcccg ccgccggcgc tgctgccgtt cgcgatcctg gtcatcggcg tcggcaactc
     5041 gatgaaggtg ttcatcatcg ccttcgtctg cctgtggccg gtgctgctga acaccatcga
     5101 cggcgtgacc ggcgtcgacc cgacgctgcg cgagacggcc cggtcgtacg gcatcccgcg
     5161 ctcccaggtg gtgcggcggg tgatgctgcc ggccgcgacg ccgcagatct tcgccggcat
     5221 gcgcaccagc ctgtcgctgg cgctgatcct gatggtgatc tcggagatgg tggcctccac
     5281 caacgggatc ggctacttcg tgctgcagtc ccagcgcacg ttcgccatcc ccgagatgtg
     5341 gtcggggatc attcttctgg ggcttctggg gtacctgttc aacggcgtgt tcgtcctgat
     5401 cgagaagcgc gtcctgcggt ggcaccgcgg cgcccgcgcc agcgcgctga gctgaggaga
     5461 gagatgctga agatcgacca cctgggcaag acctacggaa gcggcgacaa ggccgtgaag
     5521 gccatcgccg acctggactt cgaggtcaac gagaaggagc tggtgtgtgt cgtcggcccc
     5581 tcgggctgcg gcaagaccac gctgctgaag tgcatgtccg gcctgctgcc cgccacgacc
     5641 ggccaggtgc tcctgcacgg gcagcccatc gagggcccgc cggagaacat ggccctggtc
     5701 ttccaggagt acagccgttc gctgttcccg tggatgaccg tgcgccagaa cgtgtcgttc
     5761 ccgctgagcc ggcgcaagga caccgagggg cccgtggagc aggcggtcga gtcggtcggg
     5821 ctgtccgact tcctggaccg ctacccgtgg gagctctcgg gcggcatgca gcagcgagtg
     5881 gccatcgccc gggcgctggc ctaccagccc gagatcctgc tcatggacga gccgttcgcc
     5941 tcggtggacg cccagacccg cgccgacctc gaggacctgg tcctccaggt gcgcgagcag
     6001 tacggcgtga cgatcgtctt cgtgacccac gacatcgacg agtcggtgta cctgggcgac
     6061 cggatcgtgg tgctgacccc gtcgcccacc gaggtgcagg agcagctcga cgtggacctg
     6121 ccccgcccgc gcgaccagat cgacaccaag gagctgccgg agttcgccca gctgcgcggc
     6181 cacgtctacc gctcgatcaa gcggaaagag aagccggcgg tcgcatgagc ctgcacgacc
     6241 tcctcgcccg ggtcctcgag accaacccct tccagcaatc ccggct
//
LOCUS       DASUPW010000040         3506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1117440, whole genome shotgun sequence.
ACCESSION   DASUPW010000040 DASUPW010000000
VERSION     DASUPW010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117440"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="VFY44_00910"
     CDS             complement(<1..817)
                     /locus_tag="VFY44_00910"
                     /inference="COORDINATES: protein motif:HMM:NF039623.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5995 family protein"
                     /protein_id="HEX5909018.1"
                     /translation="MGRLGRLAATTAVLALGACANAHAQLPEPVNLPWERLLPSQPGR
                     ADVQPHAVPGCRDGSLACVRRVEDRLRRQFRRLDAACDHRVIASLAYLRITEMLHRDL
                     ARRSPRFFRHRKWMAYVITDFSNSYFTAFSDYERGRPVPESWRRFYDTARRGDANALQ
                     DLLLFSNAHAQHDLPLTYASMGLRTPGGVSRKHDHDGVNEINVRAIPKIKAEIERRYD
                     PTVRDVPGPLDEMGGLEPTKAWRETAWRNAERLTSAPDEAARKQVEGQIESYSA"
     gene            891..1082
                     /locus_tag="VFY44_00915"
     CDS             891..1082
                     /locus_tag="VFY44_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909019.1"
                     /translation="MATADPLATLERWCQSGAGYRVIHVSDQRAVVELLSCLGEPEDR
                     IESADPRLIERLRREAREA"
     gene            complement(1079..1555)
                     /locus_tag="VFY44_00920"
     CDS             complement(1079..1555)
                     /locus_tag="VFY44_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909020.1"
                     /translation="MPLAAWLTAAALGLLLAGGAGVGVSKLVDQEVGLSDEPITAGEQ
                     LAPLSTSAATTADAADRAAARKARARRARARRVRAARRRAAARRRAAGVPTTPAQPSR
                     PSQPNPTPPAPVQPSGGDDDAAEPPDDNSGSGGGGDDSSGKGRGRGRGGDDGPDDD"
     gene            complement(1561..2937)
                     /locus_tag="VFY44_00925"
     CDS             complement(1561..2937)
                     /locus_tag="VFY44_00925"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5909021.1"
                     /translation="MSLRTGLSLAFAYVLLLAILALGLPLALGVDDRVGTEVRSQARS
                     QADVVAATASDLLGDRRALDRLARTAGESVRGRVTVVDARGRVLADGTDPAAVGTSYR
                     ARPEFRSALAGRAVQDTRDSRTLDQTILATAVPVLRAGRPAGAVRVTQSAEAIGRAER
                     RSLLGLAALAAVVLLIGLGVGALLARGISRPLRRLDATARLIADGDLEQRARVEGSRE
                     QRSLARAFNTMTARLAESLDAQRRFVADASHQLRTPLTGLKLRMEEARQETDEPAVHE
                     EIDAGLAEVDRLTAIVEELLLLSSAGERSANPEWIDLAEAARDARLRWRGLADERGIA
                     LDVRAGPTAGSVTCSRAALDGAVDVLVENALRYSEPGTVVEILAGPDRVEVLDRGPGL
                     EEGEQELVLQRFHRGSAGRRGPKGSGLGLPIATELAREWGARVELVNREGGGARAVLS
                     WDERGDEA"
     gene            complement(2934..>3506)
                     /locus_tag="VFY44_00930"
     CDS             complement(2934..>3506)
                     /locus_tag="VFY44_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5909022.1"
                     /translation="RIEPDLVLLDLNLPDGDGRDVCRELQRRHDVPVVMLTARGSELD
                     RVVGLELGADDYVVKPFSSAEVIARIRAVLRRAQRDPERLEPMTVGALQVVPDRREVT
                     LDGAEVDLTRREYDLLERLMRDAGRVVSREDLLNDVWDTNWFGSTKTLDVHVGQLRRK
                     LGDDPAAPSYLRTVRGVGFRMSSPEEVAAT"
BASE COUNT          406 a         1332 c         1330 g          438 t
ORIGIN      
        1 ccgcggagta gctctcgatc tggccttcga cctgcttgcg cgcggcctcg tcgggcgccg
       61 aggtcagccg ctcggcgttg cgccacgcgg tctcgcgcca ggccttggtg ggctcgagcc
      121 cgcccatctc gtcgagcggg ccgggcacgt cgcgcaccgt cgggtcgtag cggcgctcga
      181 tctcggcctt gatcttcggg atcgcgcgca cgttgatctc gttcacgccg tcgtggtcgt
      241 gctttcgcga caccccgccg ggcgtccgga gccccatcga cgcgtaggtc agcggcaggt
      301 cgtgctgggc gtgcgcgttg gagaacagca acaggtcctg gagcgcgttg gcgtcgccgc
      361 ggcgggcggt gtcgtagaac cgccgccacg actcgggcac cggccgtccg cgctcgtagt
      421 cgctgaaggc ggtgaagtac gagttcgaga agtcggtgat cacgtacgcc atccacttgc
      481 ggtggcggaa gaaacgcggg ctgcggcggg ccaggtcccg gtgcagcatc tcggtgatcc
      541 gcaggtaggc cagcgaggcg atcacgcggt ggtcgcacgc ggcgtccagg cgcctgaact
      601 ggcgccgcag cctgtcctcg acacgccgga cgcacgccag gctgccgtcg cggcagcccg
      661 ggacggcgtg gggctggaca tcggcccggc ccggctggga cggcaggagc cgttcccagg
      721 gcaggttcac gggctcgggc agctgcgcgt gcgcgttcgc gcacgcaccc agcgcgagca
      781 ccgcggtcgt cgccgccaac ctccccagcc ggcccatgcg gagccctata ccccgctgga
      841 acgtgaacgt accgtggcct cgcaacggcg atctagactc ggtcgtgccg atggcgaccg
      901 ccgaccccct cgcgacgctg gagcgctggt gccagagcgg cgccggctat cgcgtgatcc
      961 acgtgtccga ccagcgcgcc gtggtcgagc tgctcagctg cctgggcgag cccgaggacc
     1021 ggatcgagtc cgccgatccg cgcctgatcg aacggctccg ccgggaggcg cgggaggcct
     1081 agtcgtcgtc ggggccgtcg tccccgccgc ggccgcgccc gcggcccttg ccgctgctgt
     1141 cgtcgccccc gccgcccgaa ccgctgttgt cgtcgggggg ctcggcggca tcgtcgtcgc
     1201 ccccgctcgg ctgcaccggg gcgggcggtg tggggttcgg ctgcgagggc cgggacggct
     1261 gcgccggcgt ggtgggcacg cccgcggccc ggcgccgggc ggccgcacgg cggcgggcgg
     1321 cgcggaccct gcgggcccgt gcccggcgcg cgcgtgcctt gcgcgccgcg gcgcgatcgg
     1381 cggcatcggc ggtggtggcg gcgctggtcg acaagggcgc cagctgttcg cccgcggtga
     1441 tcggctcgtc ggacagcccg acctcctggt cgacgagctt gctgacgccc acgccggcgc
     1501 cgccggccag cagcagtccc agcgcggcgg ccgtgagcca cgcggccagc ggcatgcgcc
     1561 tcacgcctcg tcaccccgct cgtcccagct gaggaccgcg cgggcgccgc cgccctcgcg
     1621 gttgaccagc tccacgcggg cgccccactc gcgcgccagc tcggtggcga tcggcaggcc
     1681 cagcccgctg cccttcggac cgcggcgccc ggcggacccg cggtggaagc gctggagcac
     1741 cagctcctgc tcgccctcct ccaggcccgg gccgcggtcc agcacctcga cccggtcggg
     1801 gccggccagg atctcgacga ccgtgccggg ctcggagtag cgcagcgcgt tctccaccag
     1861 cacgtcgacc gcgccgtcca gcgcggcgcg cgagcaggtc acgctgccgg cggtggggcc
     1921 ggcgcggacg tccagggcga tgccgcgctc gtcggccagg ccgcgccagc gcagccgcgc
     1981 gtcccgggcc gcctcagcca ggtcgatcca ctcggggttg gcgctgcgct cgcccgcgct
     2041 gctcagcagc agcagctcct cgacgatggc ggtcaggcgg tcgacctcgg ccagaccggc
     2101 gtcgatctcc tcgtgcacgg cgggctcgtc cgtctcctgg cgggcctcct ccatgcgcag
     2161 cttcaggccc gtgagcggcg tgcgcagctg gtgcgaggca tcggccacga accggcgctg
     2221 ggcgtccagc gactcggcca gccgggcggt catggtgttg aacgcgcgcg ccagcgagcg
     2281 ctgctcgcgc gagccctcca cccgcgcgcg ctgctccagg tcgccgtcgg cgatcagccg
     2341 ggcggtggcg tccaggcggc gcagcggccg cgagatgccg cgggccagca gcgcccccac
     2401 ccccaggccg atcaggagga cgacggcggc cagcgcggcc aggcccagca gcgaccggcg
     2461 ctcggcgcgg ccgatggcct cggcgctctg ggtcacgcgg accgcgcccg cgggccggcc
     2521 cgcacgcagc acgggcaccg cggtggccag gatcgtctgg tccagcgtgc ggctgtcgcg
     2581 ggtgtcctgc accgcccggc cggccagggc ggagcgaaac tcgggccggg cgcggtagga
     2641 ggtgcccaca gccgccggat cggtgccgtc ggccagcacc cggccgcgtg cgtcgaccac
     2701 ggtcacgcgg ccacgcaccg actcgcccgc ggtgcgtgcc aggcggtcca gcgcccggcg
     2761 gtcgcccagg aggtcggagg cggtggccgc caccacgtcg gcctgcgagc gcgcctgcga
     2821 gcgcacctcg gtgcccacgc ggtcgtccac tcccagggcc agcggcaggc cgagggccag
     2881 gatggccagc agcagcacgt aggcgaacgc cagcgacagc ccggtgcgca ggctcacgtg
     2941 gcggcgacct cctccgggga gctcatccgg aagcccacgc cgcggacggt gcgcaggtag
     3001 gagggcgcgg ccgggtcgtc ccccagcttg cgccgcagct ggcccacgtg cacgtccagg
     3061 gtcttggtcg agccgaacca gttggtgtcc cagacgtcgt tgagcaggtc ctcgcgcgag
     3121 accacgcgcc cggcgtcgcg catcaggcgc tcgaggaggt cgtactcgcg ccgggtcagg
     3181 tcgacctcgg cgccgtccag ggtcacctcg cgccggtcgg ggaccacctg cagggcgccg
     3241 accgtcatcg gctccagccg ctcggggtcg cgctgggcgc ggcgcagcac ggcacggatg
     3301 cgggcgatca cctcggccga gctgaagggc ttgaccacgt agtcgtcggc gcccagctcc
     3361 aggcccacca cgcggtcgag ctcgcttccg cgcgcggtga gcatgaccac ggggacgtcg
     3421 tgccggcgct ggagctcgcg gcagacgtcg cggccgtcgc cgtcgggcag gttcaggtcc
     3481 agcagcacca ggtcgggctc gatccg
//
LOCUS       DASUPW010000041         3332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1533288, whole genome shotgun sequence.
ACCESSION   DASUPW010000041 DASUPW010000000
VERSION     DASUPW010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533288"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..764
                     /locus_tag="VFY44_00935"
     CDS             129..764
                     /locus_tag="VFY44_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5909023.1"
                     /translation="MISVLIADDHGIVRSGLSMLIDRQSDMHVCSEAEDGIEAVEHAL
                     AQKPDVAVLDVSMPRLTGLHAARQIRAQNHDVHVLMLSMHDDDAYFFEALEAGAAGYV
                     SKRGADTDLIGAIRTVAGGRTFLNSRTQQTLMRQWLEDGRTAPEEDLTPRELEVVKLI
                     AEAYTNKQIADALKLSEKTVESHRGNVLGKLGMRDRVELVRYAIRRGLVEP"
     gene            781..954
                     /locus_tag="VFY44_00940"
     CDS             781..954
                     /locus_tag="VFY44_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909024.1"
                     /translation="MNPRVTIALMLGVAAVFAGIVALAWPGEEKASGGPDRFEGATLP
                     RGLKAPDFELTDQ"
     gene            964..1380
                     /locus_tag="VFY44_00945"
     CDS             964..1380
                     /locus_tag="VFY44_00945"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX5909025.1"
                     /translation="MTMRALRGKPVIVAFLYSTCEDTCPIQAQQIKGALNELGTDYPA
                     LAVSVDPANDSPRNTKAFNSKQRMTGRLTWVLGRRAELAPVWKAYAATGQRDDLEHNA
                     RFVLVDPKGIQRVGYPLDKVTPERVAHDMRLLAAGR"
     gene            complement(1377..3185)
                     /locus_tag="VFY44_00950"
     CDS             complement(1377..3185)
                     /locus_tag="VFY44_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5909026.1"
                     /translation="MSRGLRGIIGFGVLAALVSAAPAEAQFKRCQKGESAQCAELRVP
                     LDRAGQVKGSVKISALRLPARRGPRTGTVMLLPGGPGQGALEALLGSIELMVRLPTYD
                     FVAFDPRGTGESSPLRCKALKTGRESTAPQRCARELGLRRGYYRSYDTVEDIEAVRVA
                     LGSPRLTLLSTSYGGRVAGEYVRRHPESVARQIMDSPVPVTGADPFGREAPAAQRRVL
                     DTVRRGGARSVARLAARLARRPLRGRVITAKGGSRRARLSQAGLYAALVGGDLDSILR
                     GQLPAAAASAVRGDPAPLLRLTGAASSESEDDGVSVPVFLATTCSESALPWDTAAPPT
                     AARERQATVELRRLGRAPFAPFSPLTVVGTTGLIGQCLAWPAVPKTPPLPAGSQNSGV
                     PTLVLQGQEDTRTPLEQSRGIAATYTGSRLLTVPYAGHAVLGSDSSGCSGDAAVAFLR
                     GAVPPDRCPARSRPLEDPVVSRFPRTLRSLRGSLRRRSINAGILTVSDVLLNFPAGRK
                     GVGGLRGGRATARKRTIVLDDYEYLSGVRVTGTIRLKGEKVSGRVRIRGGGALTVLQT
                     YRGARVSTLFGTGRTASAASVSRWTPPPRRPAPRLP"
BASE COUNT          463 a         1224 c         1254 g          391 t
ORIGIN      
        1 gacccggtgg ccgcccgtga cggcgggctc gggctcgaag gcatggccga gcgggccaag
       61 ctggtcggcg gcgagctgga cctgcgatcg gcgcccggag cgggcaccga agtgacactg
      121 aggctgccgt gatctccgtc ctgatcgccg acgaccacgg catcgtccgc tcggggctgt
      181 cgatgctgat cgaccgccag tccgacatgc acgtgtgctc cgaggcggag gacggcatcg
      241 aggccgtgga gcacgccctg gcccagaagc cggacgtggc cgtgctggac gtgtccatgc
      301 cgcgcctgac cggcctgcac gcggcgcgcc agatccgcgc gcagaaccac gacgtccatg
      361 tgctgatgct gtcgatgcac gacgacgacg cctacttctt cgaggcgctc gaggccggcg
      421 ccgccggcta cgtgtccaag cgcggcgccg acaccgacct gatcggcgcc atccgcaccg
      481 tcgccggcgg gcgcacgttc ctgaactccc gcacccagca gacgctgatg cgccagtggc
      541 tcgaggacgg ccggaccgcg cccgaggagg acctgacccc gcgcgagctg gaggtggtca
      601 agctgatcgc cgaggcctac accaacaagc agatcgccga cgcgctgaag ctgtcggaga
      661 agaccgtgga gtcccaccgc ggcaacgtcc tgggcaagct cggcatgcgc gaccgggtcg
      721 agctggtccg gtacgccatc aggcgcgggc tcgtggagcc ctagccctac cctgcggggc
      781 gtgaacccgc gcgtgaccat cgccctgatg ctgggcgtcg cggccgtctt cgccggcatc
      841 gtcgcgctgg cgtggcccgg cgaggagaag gcctccggcg ggccggaccg gttcgagggc
      901 gccaccctgc cccgcgggct gaaggccccc gacttcgagc tgaccgacca gtagggccgg
      961 cgcgtgacca tgcgcgcgct gcgcggcaag ccggtgatcg tggccttcct ctattccacc
     1021 tgcgaggaca cctgcccgat ccaggcgcag cagatcaagg gcgcgctgaa cgagctgggc
     1081 accgactacc cggcgctggc ggtgtcggtg gaccccgcca acgactcgcc gcgcaacacg
     1141 aaggcgttca acagcaagca gcgcatgacc ggccgcctga cctgggtgct gggccggcgg
     1201 gccgagctgg cgccggtgtg gaaggcgtac gccgccaccg gccagcgcga cgacctggag
     1261 cacaacgccc gcttcgtgct ggtggacccc aagggcatcc agcgcgtggg ctacccgctg
     1321 gacaaggtca cgcccgagcg ggtggcgcac gacatgaggc tgctggccgc gggccgttag
     1381 ggcaggcggg gcgcgggccg gcgcgggggc ggcgtccagc ggctgaccga cgccgccgac
     1441 gcggtccgcc cggtgccgaa gagcgtggac acccgcgcgc cgcggtaggt ctgcagcacc
     1501 gtcagggcgc cgccgccgcg gatgcgcacg cggcccgaga ccttctcgcc cttgagccgg
     1561 atcgtgccgg tgacgcgcac gccgctcagg tactcgtagt cgtccagcac gatcgtgcgc
     1621 ttgcgcgcgg tggcgcggcc gccgcgcagc ccgccgacgc ccttgcgccc ggcggggaag
     1681 ttcagcagca cgtcggagac ggtcaggatg cccgcgttga tgctgcgccg gcgcaggctg
     1741 ccccgcagtg agcggagggt gcgcgggaag cgcgagacca ccgggtcctc gagcggccgc
     1801 gagcgcgccg gacaccgatc cgggggcacg gcgccgcgca ggaacgccac ggcggcgtcg
     1861 ccggagcagc ccgaggagtc cgagcccagg acggcgtggc cggcgtaggg caccgtcagc
     1921 aggcggctgc cggtgtacgt cgcggcgatg ccgcggctct gctcgagcgg cgtccgggtg
     1981 tcctcctggc cctggagcac cagcgtcggg acgcccgagt tctggctgcc cgccggcagc
     2041 ggcggggtct tgggcaccgc gggccaggcc aggcactggc cgatcaggcc ggtcgtcccg
     2101 accacggtga gcggggagaa cggcgcgaac ggcgcccgcc ccaggcgcct cagctccacg
     2161 gtcgcctggc gctcgcgagc ggccgtcggc ggcgcggcgg tgtcccaggg cagcgccgac
     2221 tcgctgcagg tggtggccag gaacaccggc acggacacgc cgtcgtcctc ggactccgag
     2281 ctcgcggcgc cggtcaggcg caatagcggg gcgggatcgc cacggacggc ggaggcggcg
     2341 gccgccggca gctggccgcg caggatcgag tccaggtctc cgcccaccag cgccgcgtac
     2401 agcccggcct gcgacaggcg ggcgcggcgc gagccgccct tggcggtgat cacgcgcccg
     2461 cgcaacgggc gccgtgccag ccgggcggcc agccgggcca ccgaccgcgc gcctccccgc
     2521 cggaccgtgt ccagcacgcg ccgctgggcc gcgggcgcct cgcggccgaa ggggtcggcg
     2581 ccggtcacgg gcacgggcga gtccatgatc tgccgcgcga ccgactcggg gtggcggcgt
     2641 acgtactcgc cggccacgcg gccgccgtag gaggtcgaga gcagcgtcag ccggggcgaa
     2701 ccgagcgcca cccggaccgc ctcgatgtcc tcgacggtgt cgtagctgcg gtagtagccg
     2761 cggcgcaggc cgagctcgcg ggcgcagcgc tgcggggcgg tcgactcacg cccggtcttc
     2821 agcgccttgc agcgcagcgg gctcgactcg ccggttccgc gcgggtcgaa cgccacgaag
     2881 tcgtaggtgg gcaggcggac catcagctcg atcgagccca gcagcgcctc gagcgcgccc
     2941 tgcccgggcc cgccgggaag caacatgacc gtgccggtcc gcgggcccct gcgcgcgggc
     3001 agccgtagcg cggagatctt caccgacccc ttcacctggc ccgcccggtc cagcggcacc
     3061 cggagctcgg cgcactgcgc gctctcgccc ttctggcacc gcttgaactg cgcctcggcg
     3121 ggcgcggcgg acaccagcgc cgcgagcacc ccgaacccga tgatcccccg tagccccctg
     3181 ctcatggcgg ggcaccctac agaggccggt gcggcggtga gccggttcgc tccgggcgat
     3241 tcccgcgggc atcgttaacc tagtccggcg gtaggaatag atcctgatga tgttctcaac
     3301 caaagccgag tacggggtgc gcgtgatggc gc
//
LOCUS       DASUPW010000042         3070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_979203, whole genome shotgun sequence.
ACCESSION   DASUPW010000042 DASUPW010000000
VERSION     DASUPW010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979203"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VFY44_00955"
     CDS             <1..640
                     /locus_tag="VFY44_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5909027.1"
                     /translation="FDVAGSIGRTVELDDLCRRAVLAGAADLPPDALLFVNVAPQSLD
                     GQRLAGDALAAEVRAAGLEPERVVLEITERFDGRIDRIVAEATRLRALGFKLALDDVG
                     AGNSGLQMMRELELDFVKIDRMVLVRAVTDVTARAVLVSVIAFARETGTFVIAEGIED
                     EEMLALARWPRPAEADLLGAQGLQGYLLGRPGALGDAPEPARTQEEQVLGSL"
     gene            664..1530
                     /locus_tag="VFY44_00960"
     CDS             664..1530
                     /locus_tag="VFY44_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5909028.1"
                     /translation="MATTVESAPDPTLDSLQLFLDGIAKTPLLTASEEVRLAKRVERG
                     DLDAKEHMVQANLRLVVSIAKRYRNQGLDFMELIQEGSIGLVRAVEKFDYRKGFKFST
                     YATWWIRQAISRAIADKSRTIRIPIHINQTLQKLDGAQRRLQSVGEGNPSIEEITAAA
                     NVDIDVEEAEFLMRASRAPVSLDKPVGGGEEDAAELGHLIPDDRAESPFETAAESMTR
                     SRLAEALENLSYRERRVVELRHGLFGEHPRTLDEVGRMFGLTRERTRQVEEHALRKLA
                     SLAEARGLRDAA"
     gene            1550..1990
                     /locus_tag="VFY44_00965"
     CDS             1550..1990
                     /locus_tag="VFY44_00965"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5909029.1"
                     /translation="MAVKTEAVGKAYPPFEYEVGKEKIAEYARAIGAENPVHFDREAA
                     REAGYRDVVAPPMFAVVYSAGSVAPGIFDPDVGINFATMVHGGQEFVWDEPVVSGDVI
                     STTTTVKDVSEKDGKGFYVFESVSTNQDGREVVRGTWTNIVRGV"
     gene            1987..2343
                     /locus_tag="VFY44_00970"
     CDS             1987..2343
                     /locus_tag="VFY44_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX5909030.1"
                     /translation="MTELRVTPDKYLPHRYAGASGDFNPIHIDTEFAQQVGLPRNILH
                     GLYSMALVARAATGDGDPLALKRLSVQFRGMGVPEEEIVVTGSERDDGVVELEAAQGD
                     NRIIRNAEAEIRSTSP"
     gene            2357..>3070
                     /locus_tag="VFY44_00975"
     CDS             2357..>3070
                     /locus_tag="VFY44_00975"
                     /inference="COORDINATES: protein motif:HMM:NF013768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909031.1"
                     /translation="MQISVRQSTLLRKVVEGYKQLDAPVGSKWLAGQDDVPWGPSTVR
                     SELARLEEFGLLEHPHTSAGRRPTDSGYRYYVDELLDKGDLPAVRAPLELSDMRREVD
                     EAMRATTEQLSQVTDLLALVSAPSIGTATIHRVEVLLLQPQVAMVVVITSTGGVTKRV
                     ISYDEPLDEGLLAWAGSYLNEVLGGMDVGARMLGGKLADPELSARERDFLATLAPAFT
                     ELEDSAGDTRFVDGAARLVS"
BASE COUNT          465 a         1088 c         1106 g          411 t
ORIGIN      
        1 cttcgacgtg gccggcagca tcggccgcac ggtggagctg gacgacctgt gccgccgcgc
       61 ggtcctggcc ggcgccgccg acctgccccc ggacgcgctg ctgttcgtga acgtcgcgcc
      121 ccagagcctg gacggccagc ggctggccgg tgacgcgctg gcggccgagg tccgcgcggc
      181 gggcctggag cccgagcggg tcgtgctgga gatcaccgag cgcttcgacg gccggatcga
      241 ccggatcgtg gccgaggcca cgcggctgcg cgcgctgggc ttcaagctcg ccctggacga
      301 cgtcggcgcc ggcaactcgg gcctgcagat gatgcgcgag ctcgagctgg acttcgtgaa
      361 gatcgaccgg atggtcctgg tccgggccgt cacggacgtg accgcacggg ccgtgctggt
      421 gtcggtgatc gcgttcgccc gcgagaccgg gacattcgtg atcgccgagg gcatcgagga
      481 cgaggagatg ctggcgctgg cgcgctggcc ccgcccggcc gaggccgacc tgctgggcgc
      541 gcagggcctc caggggtatc tcctgggtcg tccgggggcg ctcggggacg ccccggagcc
      601 ggcgcgcacg caggaggaac aggtcttagg aagcctttag ggcacggccg gagaatcgtc
      661 gccgtggcca ccaccgtaga aagcgctccc gatccgaccc tcgactcgct ccagctgttc
      721 ctggacggca tcgccaagac gccgctcctg accgcgagcg aggaggtccg gctggccaag
      781 cgcgtcgagc gcggcgacct ggacgccaag gagcacatgg tccaggccaa cctgcgcctg
      841 gtggtgtcga tcgccaagcg ctaccgcaac cagggcctgg acttcatgga gctgatccag
      901 gagggctcca tcggcctggt gcgcgcggtc gagaagttcg actaccgcaa ggggttcaag
      961 ttctcgacgt acgccacctg gtggatccgc caggccatct cgcgcgccat cgccgacaag
     1021 tcgcgcacca tccggatccc gatccacatc aaccagacgc tccagaagct ggacggcgcc
     1081 cagcgccgcc tccagagcgt gggtgagggc aacccctcga tcgaggagat caccgcggcc
     1141 gcgaacgtgg acatcgacgt cgaggaggcc gagttcctga tgcgcgcctc gcgcgcgccg
     1201 gtctcgctgg acaagccggt gggcgggggc gaggaggacg cggccgagct gggccacctg
     1261 atcccggacg accgcgccga gtcgccgttc gagaccgcgg ccgagagcat gacccgcagc
     1321 cgcctggccg aggcgctgga gaacctgtcc taccgcgagc gccgggtggt cgagctgcgc
     1381 cacggcctgt tcggcgagca cccccgcacg ctcgacgagg tcggccgcat gttcggcctc
     1441 acccgcgagc gcacgcgcca ggtcgaggag cacgcgctgc gcaagctcgc ctcgctggcc
     1501 gaggcccgcg gcctgcgcga cgccgcctag cgttagcctc ccggcccacg tggccgtgaa
     1561 gaccgaagcc gttggcaagg cctacccgcc gttcgagtac gaggtgggca aggagaagat
     1621 cgccgagtac gcgcgggcga tcggcgccga gaaccccgtc cacttcgacc gcgaggccgc
     1681 ccgcgaggcg ggctaccgcg acgtggtcgc cccgcccatg ttcgctgtcg tctactccgc
     1741 cggctcggtg gcgcccggca tcttcgaccc cgacgtgggg atcaacttcg cgacgatggt
     1801 ccacgggggc caggagttcg tgtgggacga gccggtggtc tccggcgacg tgatctccac
     1861 gaccaccacc gtcaaggacg tctccgagaa ggacggcaag ggcttctacg tgttcgagtc
     1921 ggtgtccacc aaccaggacg gccgcgaggt cgtgcgcggc acctggacca acatcgtgag
     1981 gggcgtatga ccgagctcag ggtcaccccc gacaagtacc tgccccaccg ctacgcgggg
     2041 gcgtcgggtg acttcaaccc gatccacatc gacaccgagt tcgcgcagca ggtcggcctg
     2101 ccgcgcaaca tcctgcacgg gctctactcg atggcgctcg tggcgcgcgc ggccacgggc
     2161 gacggcgatc cgctcgcgct gaagcggctg tcggtgcagt tccgcggcat gggcgtcccc
     2221 gaggaggaga tcgtcgtcac gggcagcgag cgcgacgacg gcgtcgtgga gctcgaggcg
     2281 gcccagggcg acaaccggat catccgcaac gccgaggccg agatccgcag caccagcccc
     2341 taagctggac ggttcgatgc agatctccgt tcgccagagc accctgctcc gcaaggtggt
     2401 cgagggctac aagcagctcg acgcgccggt ggggtccaag tggctcgccg gccaggacga
     2461 cgtgccctgg ggcccgtcga ccgtccgctc cgagctcgcg cgcctggagg agttcggcct
     2521 gctcgagcat ccccacacct ccgccggccg ccggcccacc gactcgggct accgctacta
     2581 cgtggacgag ctgctggaca agggcgacct gccggcggtg cgcgcgccgc tggagctcag
     2641 cgacatgcgc cgcgaggtcg acgaggccat gcgggcgacc accgagcagc tgtcgcaggt
     2701 caccgacctg ctggcgctgg tctcggcgcc ctcgatcggc acggccacca tccaccgcgt
     2761 cgaggtgctg ctgctccagc cgcaggtggc catggtcgtg gtcatcacca gcaccggggg
     2821 cgtgaccaag cgcgtgatct cctacgacga gccgctggac gagggcctgc tggcctgggc
     2881 cggcagctac ctcaacgagg tgctgggcgg gatggacgtg ggcgcgcgga tgctgggcgg
     2941 caagctggcc gaccccgagc tgagcgcgcg cgagcgcgac ttcctggcca cgctggcccc
     3001 cgcgttcacc gagctcgagg actccgccgg cgacacccgg ttcgtggacg gggcggcacg
     3061 gctggtctcc
//
LOCUS       DASUPW010000043         2161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1949237, whole genome shotgun sequence.
ACCESSION   DASUPW010000043 DASUPW010000000
VERSION     DASUPW010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1949237"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VFY44_00980"
     CDS             <1..426
                     /locus_tag="VFY44_00980"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEX5909032.1"
                     /translation="REQKEDDDREVPRRDGNALALLRSVGGFQAFMRSAAAPPNAGPV
                     TRFLLFERDFPDSVAASVESLHGALSRAEQDPLEAPPVLRLARISADLKFRSGNSETG
                     ADLAPAFREVQHELDAADEEIDGRYFQGQGMTRQKAGWA"
     gene            423..1289
                     /locus_tag="VFY44_00985"
     CDS             423..1289
                     /locus_tag="VFY44_00985"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX5909033.1"
                     /translation="MKYSIRYLTEYRYDSPVFDQHNALRVRPAATATQRVDAFELRID
                     PSARLHTYTDYFGTDVIEFNLIEGHERLSIEARARVTNEQPPEPAGGSWAALDSEEYR
                     EAAGEFVLPAGDQPTGPAMDELAAAIRRDTPAETLVALTESIPARFEYKKGATYVGST
                     VADLLEGGAGVCQDFVHLGLMLLRSHGIAARYVSGYLFAAKEKGGDSAEVDTHAWLEA
                     LLPGPDGEEPSWTGADPTNRGLAGQQHVKIGHGRFYSDVPPIKGVFRGSAQAKLDATV
                     QMTRIENGNSAS"
     gene            complement(1286..1807)
                     /locus_tag="VFY44_00990"
     CDS             complement(1286..1807)
                     /locus_tag="VFY44_00990"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEX5909034.1"
                     /translation="MLREDRGAEFTTEGIWLWRRHSFCVGRRVAPALTPRRLARLHQV
                     QAREPVCVLEDEGRRWWWYSDRFFSEADDLSPADVTALVADRDRRRARRLQRAHAEME
                     AEADAARRRAPIPREVRRTVWARDGGRCVQCGAGFDLQYDHVIPHAMGGADTEANLQL
                     LCGDCNRAKGGGL"
     gene            complement(1817..>2161)
                     /locus_tag="VFY44_00995"
     CDS             complement(1817..>2161)
                     /locus_tag="VFY44_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5909035.1"
                     /translation="AGVRELTGLGEAEARTALAAFGLGAREATRPAATLSPGERTRAE
                     LAVVAHRRATCLLLDEPTNHLDVASLEVLEDALADWPGALVVATHDTSLRRSLALERS
                     VQLGFQAIRADS"
BASE COUNT          299 a          830 c          774 g          258 t
ORIGIN      
        1 cgggagcaga aggaggacga cgaccgcgag gtcccccgcc gcgacggcaa cgcgctggcg
       61 ctgctgcgct cggtcggtgg cttccaggcc ttcatgcgct ccgcggccgc gccgcccaac
      121 gccggcccgg tgacccgctt cctgctgttc gagcgcgact tccccgactc ggtggccgcg
      181 tcggtcgagt cgctgcacgg cgcgctgtcg cgggccgagc aggacccgct ggaggcgccg
      241 ccggtgctgc gcctggcgcg catctcggcc gacctgaagt tccgcagcgg caactccgag
      301 accggcgccg acctggctcc cgcgttccgc gaggtccagc acgagctgga cgccgccgac
      361 gaggagatcg acgggcgcta cttccagggc cagggcatga cgcggcagaa ggcgggctgg
      421 gcgtgaagta ctcgatccgc tacctgaccg agtaccgcta cgacagcccg gtgttcgacc
      481 agcacaacgc gctgcgcgtg cgcccggcgg ccacggccac ccagcgcgtc gacgccttcg
      541 agctgcggat cgaccccagc gcgcgcctgc acacctacac cgactacttc gggaccgacg
      601 tgatcgagtt caacctgatc gagggccacg agcggctgtc gatcgaggcc cgggcacgcg
      661 tgaccaacga gcagccgccc gaaccggcgg gcggctcgtg ggcggccctg gacagcgagg
      721 agtaccgcga ggccgcgggc gagttcgtgc tgccggccgg cgaccagccg accggccccg
      781 ccatggacga gctggcggcg gccatccgcc gcgacacccc ggccgagacg ctggtggcgc
      841 tgaccgagtc catcccggcc cggttcgagt acaagaaggg cgccacctac gtcggctcga
      901 ccgtggccga cctgctggag ggcggcgccg gcgtctgcca ggacttcgtc cacctcgggc
      961 tgatgctgct gcgctcgcac ggcatcgccg ctcggtacgt gtcgggctac ctgttcgccg
     1021 ccaaggagaa gggcggcgac tcggccgagg tggacaccca cgcctggctg gaggcgctgc
     1081 tgcccggccc cgacggcgag gagccgtcgt ggaccggcgc cgacccgacc aaccgcggcc
     1141 tggccgggca acagcacgtg aagatcggcc acgggcgctt ctactccgac gtgccgccca
     1201 tcaagggcgt cttccgcggc tccgcacagg ccaagctgga cgccacggtg cagatgacgc
     1261 ggatcgagaa cggcaacagc gcctcctaga ggccgccgcc cttcgcgcgg ttgcagtcgc
     1321 cgcacagcag ctgcaggttc gcctcggtgt cggcgccgcc catcgcgtgc gggatcacgt
     1381 ggtcgtactg gaggtcgaag ccggcgccgc actgcacgca gcgcccgccg tcccgcgccc
     1441 acaccgtgcg ccggacctca cgcgggatcg gcgcccgccg ccgggccgca tcggcctccg
     1501 cctccatctc ggcatgggcg cgctggagcc tgcgcgcgcg gcggcggtca cggtcggcca
     1561 ccagcgcggt cacgtcggcg ggcgacaggt cgtcggcctc actgaagaag cggtccgagt
     1621 accaccacca gcgccggccc tcgtcctcga gcacgcacac gggctcgcgc gcctggacct
     1681 ggtgcaggcg ggccaggcgg cgcggggtca gcgccggcgc gacccggcgg ccgacgcaga
     1741 agctgtgccg gcgccacagc cagatgccct cggtcgtgaa ctcggcgcct ctgtcctctc
     1801 tgagcatgcg gccacgctag gaatccgccc ggatcgcctg aaagcccagt tgcacgctgc
     1861 gctccagcgc cagcgagcgc cgcaggctgg tgtcgtgggt ggccaccacc agcgcacccg
     1921 gccagtccgc cagcgcgtcc tccagcacct ccagcgaggc cacgtccagg tggttggtgg
     1981 gctcgtccag cagcaggcac gtggcgcgcc ggtgggccac cacggccagc tccgcgcggg
     2041 tgcgctcgcc cggcgacagc gtcgccgcgg gacgggtggc ctcgcgcgcg ccgaggccga
     2101 acgcggcaag cgccgtccgg gcctcggcct cccccaggcc cgtcagctcg cggacgccgg
     2161 c
//
LOCUS       DASUPW010000044         6221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_702601, whole genome shotgun sequence.
ACCESSION   DASUPW010000044 DASUPW010000000
VERSION     DASUPW010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702601"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1203)
                     /locus_tag="VFY44_01000"
     CDS             complement(<1..1203)
                     /locus_tag="VFY44_01000"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX5909036.1"
                     /translation="MPQHDVLVIGAGLAGQRAALAAAEAGASVGIISKVHPVRSHSNA
                     AAGGINAALNPEDSWESHAYDTVKGSDFLGDQDAIEIMCKEAPDEVLQLEHWGVTFHR
                     NEEGKLGTRAFGGASAARTYYIADITGQALLHVLYEQLMKHDEVDRYEEWFTTALVRD
                     ESGRIAGAVTRNIRDGELQLFTAKSVILATGGNGQIFTPTTNALICTGDGIAMAYHAG
                     APLMDMEMIQYHPTTLAGVGLLITEGARGEGAHLYNAEGERFMEKYAPNKMELASRDV
                     VSRAEQTEIDEGRGFPDGTVALDITVVGAKRIHESLREIVNVGRDFAGTDITKEPIHI
                     KPGNHYVMGGIKTDVHGQTEVPGLYAAGECACVSVHGGNRLGANSLLDTLIFGRRSGE
                     HAAEVAKGM"
     gene            complement(1299..2465)
                     /locus_tag="VFY44_01005"
     CDS             complement(1299..2465)
                     /locus_tag="VFY44_01005"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5909037.1"
                     /translation="MFGGGGSIQLARVFGIRVGVEPSWFLILFVIIWSLTGYYGDLFP
                     DQGTSSFVLAVVSALLFFGSIVLHELGHAVVAMRNGIKIEGIDLWLFGGVAKLSKDTD
                     SAGVEFRVAAAGPAVTALIAAVCFGAGTLISGTGEAADSARFDAVAGDKTLAVLGYLT
                     FINVILLVFNLVPAFPLDGGRIARAIAWKITGNRNTATRFAARMGRVISYLMMGLGGY
                     LIVATSDFITGVWLILIGLFVGQAAKAAEQQTDVADRIEGLRVADVMDDEPVAIPADL
                     TLDRAEEEFFLRYRYPWFPVVGAGNRFVGLVTQGSVESLPEAVRGGRTVASVMAADGE
                     GESRMRVNADEPLEALLGLEGLVRLGAIMAVDGEGRLRGIVTADQVRRALAPAA"
     gene            2506..4683
                     /locus_tag="VFY44_01010"
     CDS             2506..4683
                     /locus_tag="VFY44_01010"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:TIGR00178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX5909038.1"
                     /translation="MKIIYTETDEAPALATRSLLPIVRRFAASAGIEIELRDISLAGR
                     ILAQFPDLLSDDQQVPDALTELGELAKTPDANIIKLPNISASVPQLKSAVKELQEQGF
                     PIPDYPENPSTDEERDARGRYDTVKGSAVNPVLREGNSDRRAPESVKSYVRSHPHSMG
                     EWSSDSKTHVATMGDGDFRSTEKSVTVDAEDDVRIEHVAGDGTVTVLKESTPLLAGEV
                     IDASVMRRKELRAFLDQQLKDARESGVLFSVHLKATMMKVSDPIIFGHAVGAMFGELF
                     EKRAGINPDDGLAALASADDEAQGEIDAVLAEGPALYMVDSDRGVTNLHVPSDVIIDA
                     SMPALIRSSGKGWDADGGQQDTKCVIPDSSYAALYQETIDHCREHGAFDPATMGTTPN
                     VGLMAQKAEEYGSHDKTFEIEGPGTVRVVDRAGDTLLEHEVDTGDIWRMCQTKDAPIQ
                     DWVRLAVSRAQATGAPAVFWLDETRAHDAQLLEKVRPALEEIDTEGAQIEIKPVAEAT
                     RFTLERARAGEDTISVTGNVLRDYLTDLFPILELGTSAKMLSIVPLMGGGGLFETGAG
                     GSAPKHVQQFVKENHLRWDSLGEFLALAVSFEFLADKTDNARARVLGATLDRATGRLL
                     EEGRSPLRKAGELDNRGSHAYLAVYWAAELAAQTDDADLAEAVAPLAERLAADEEKIV
                     EELNGVQGEPVDIGGYFHPDTDKVVAAMRPSQTLNAALDDLDA"
     gene            4694..5686
                     /locus_tag="VFY44_01015"
     CDS             4694..5686
                     /locus_tag="VFY44_01015"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF003916.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX5909039.1"
                     /translation="MTSPVRVAVTGAAGQIGYALLFRIASGQLLGPDTPVHLSLLEIP
                     DAVKAAEGTAMELDDSAFPLLAGIDISDDAKTAFDGVNVALLVGARPRTKGMERADLL
                     EANGGIFKPQGEALAAGAADDLKVLVVGNPANTNALIAQQNADGVPAERFTAMTRLDQ
                     NRAMTQLAKKAGAPVGEVTNLAVWGNHSPSMYPDVFNAKVGGSDAWEAAGKDESWLKD
                     DFIPTVGKRGAAIIEARGASSAASAANAAIDHVYDWVNGTADGDFVSMAVPSDGSYDV
                     PEGIISSFPVTTSGGSYEIVQGLDINETSRGYIDATAQELVEEREAVQKLGLVS"
     gene            5723..>6221
                     /locus_tag="VFY44_01020"
     CDS             5723..>6221
                     /locus_tag="VFY44_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909040.1"
                     /translation="MARAPTGAAAQPGGTLPGAVAVLALVPLAGFVLLLAAPSADVQW
                     EDHRAHFWLVVLTALAGASFAYATGETARRRGDSRLFLIYLAFLAGGGFLAVHALATP
                     GVLLDGPNAGFTLATPIGLLAAAALAALSTRDPQPAAGSPALRALQLGLIAVMAVWVA
                     ASLSEV"
BASE COUNT          929 a         2246 c         2165 g          881 t
ORIGIN      
        1 catgcccttg gccacctcgg cggcgtgctc gcccgagcgg cggccgaaga tcagcgtgtc
       61 cagcagcgag ttggcgccca ggcggttgcc gccgtgcacc gagacacagg cgcactcgcc
      121 ggcggcgtac aggcccggca cctcggtctg gccgtggacg tcggtcttga tcccgcccat
      181 cacgtagtgg ttgcccggct tgatgtggat gggctccttg gtgatgtcgg tgccggcgaa
      241 gtcgcggccc acgttcacga tctcgcgcag cgactcgtgg atgcgcttgg cgccgaccac
      301 ggtgatgtcc agcgccacgg tgccgtcggg gaagccacgg ccctcgtcga tctcggtctg
      361 ctccgcacgg gagacgacgt cgcgcgaggc cagctccatc ttgttgggcg cgtacttctc
      421 catgaagcgc tcgccctcgg cgttgtacag gtgggcgccc tcgccgcggg cgccctcggt
      481 gatcagcagc ccgactccgg ccagcgtggt ggggtggtac tggatcatct ccatgtccat
      541 cagcggcgcg cccgcgtggt aggccatggc gatcccgtcg cccgtacaga tcagggcgtt
      601 ggtggtgggg gtgaagatct gcccgttgcc gccggtggcc aggatcaccg acttggcggt
      661 gaacagctgg agctcgccgt cgcggatgtt gcgcgtgacc gcgccggcga tccggccgct
      721 ctcgtcacgc accagcgcgg tggtgaacca ctcctcgtac cgatcgacct cgtcgtgctt
      781 catcagctgc tcgtagagca cgtgcagcag ggcctggccc gtgatgtcgg cgatgtagta
      841 ggtgcgggcg gccgaggcgc caccgaacgc gcgggtgccg agcttgccct cctcgttgcg
      901 gtggaaggtc acgccccagt gctcgagctg gagcacctcg tccggggcct ccttgcacat
      961 gatctcgatg gcgtcctggt cgccgaggaa gtccgagccc ttgacggtgt cgtaggcgtg
     1021 cgactcccac gagtcctccg ggttcagcgc ggcgttgatg ccgccggcgg ccgcgttcga
     1081 gtgcgagcgc accgggtgga ccttgctgat gatgccgacc gatgcacctg cctcggcggc
     1141 ggccagtgct gcgcgttggc cggcgagtcc ggctccgatg acgagtacgt cgtgttgggg
     1201 catggtgggg gtgaggcgac ggcgactcta cctgaccgtc tccaggtcct ttcttccgcg
     1261 gtccactgag caatctacgc gtgcgagcgc ccgggagcct acgccgcggg cgccaaggcg
     1321 cgtcgaacct ggtccgcggt gacgattcca cgcagccggc cctcgccgtc cacggccatg
     1381 atcgcgccca gccggaccag cccctccagg cccagaagcg cctccagcgg ctcgtcggcg
     1441 ttcacgcgca tccggctctc gccctcgccg tcggcggcca tgaccgaggc cacggtccgg
     1501 ccgccgcgca cggcctcggg cagcgactcc acgctgccct gggtcaccag ccccacgaac
     1561 cggttgccgg cgcccacgac cgggaaccac gggtagcggt agcgcaggaa gaactcctcc
     1621 tcggcccggt ccagcgtcag gtcggccggg atggccaccg gctcgtcgtc catcacgtcg
     1681 gccacgcgca gcccctcgat ccggtcggcc acgtcggtct gctgctcggc cgccttggcg
     1741 gcctggccca cgaacaggcc gatcaggatc agccagacgc cggtgatgaa gtcgctggtg
     1801 gccacgatca ggtacccgcc caggcccatc atcaggtacg agatcacgcg gcccatccgg
     1861 gcggcgaagc gggtggcggt gttgcggttg ccggtgatct tccaggcgat ggcccgcgcg
     1921 atccggcccc cgtccagggg gaaggcgggc accaggttga acaccagcag gatcacgttg
     1981 atgaaggtca ggtagcccag cacggccagc gtcttgtccc cggcgacggc gtcgaagcgg
     2041 gccgagtccg ccgcctcacc cgtgcccgag atcagcgtcc ccgcgccgaa gcagaccgcg
     2101 gcgatcagcg ccgtgaccgc ggggccggcg gcggcgacac ggaactcgac gccggccgag
     2161 tcggtgtcct tggacagctt ggccacgccg ccgaacagcc acaggtcgat gccctcgatc
     2221 ttgatgccgt tgcgcatggc caccaccgcg tggcccagct cgtgcaggac gatgctgccg
     2281 aagaagagca gcgccgagac caccgccagc acgaacgagc tcgtgccctg gtcggggaac
     2341 aggtcgccgt agtagccggt cagcgaccag atgatcacga acaggatcag gaaccagctg
     2401 ggctccacgc ccacgcggat gccgaacact cgcgcgagct ggatggaacc gccaccgccg
     2461 aacatgcagc ggatggtggc agctatgtag tttcagacgc cgaccatgaa gatcatctac
     2521 accgagaccg acgaggcccc cgctctggcg actcgctcgc tgctgccgat cgtgcgcagg
     2581 ttcgccgcct ccgccgggat cgagatcgag ctccgcgaca tctcgctggc cgggcggatc
     2641 ctggcccagt tccccgatct gctcagcgac gaccagcagg tgccggacgc gctgaccgag
     2701 ctcggcgagc tggccaagac gcccgacgcc aacatcatca agctgccgaa catcagcgcc
     2761 tcggtgcccc agctcaagag cgccgtcaag gagctgcagg agcagggctt cccgatcccc
     2821 gactaccccg agaacccgtc gaccgacgag gagcgcgacg cccgcgggcg ctacgacacc
     2881 gtcaagggat cggccgtgaa cccggtgctg cgcgagggca actcggaccg ccgcgcgccc
     2941 gagtcggtca agtcgtacgt gcgctcgcac ccgcactcca tgggcgagtg gtcctcggac
     3001 tccaagaccc acgtggccac gatgggcgac ggcgacttcc gctcgaccga gaagtcggtg
     3061 acggtggacg ccgaggacga cgtccggatc gagcacgtgg ccggggacgg gacggtcacg
     3121 gtgctcaagg agtccacgcc gctgctggcc ggcgaggtca tcgacgcgtc ggtcatgcgc
     3181 cgcaaggagc tccgggcctt tctcgaccag cagctgaagg acgcgcgcga gtccggcgtc
     3241 ctgttctcgg tccacctgaa ggcgacgatg atgaaggtct ccgacccgat catcttcggg
     3301 cacgccgtcg gcgcgatgtt cggcgagctg ttcgagaagc gcgccggcat caaccccgac
     3361 gacggcctgg ccgcgctggc ctccgcggac gacgaggccc agggcgagat cgacgccgtg
     3421 ctggccgagg ggccggcgct ctacatggtc gactccgacc ggggcgtgac caacctgcac
     3481 gtgcccagcg acgtgatcat cgacgcctcg atgccggcgc tgatccggtc ctccggcaag
     3541 ggctgggacg ccgacggcgg ccagcaggac accaagtgcg tgatccccga cagctcgtac
     3601 gcggcgctgt accaggagac gatcgaccac tgccgcgagc acggcgcgtt cgacccggcc
     3661 accatgggca cgacccccaa cgtcggcctg atggcgcaga aggccgagga gtacggctcg
     3721 cacgacaaga ccttcgagat cgagggcccc ggcacggtgc gcgtggtcga ccgggcgggg
     3781 gacaccctgc tcgagcacga ggtcgacacc ggcgacatct ggcgcatgtg ccagaccaag
     3841 gacgccccga tccaggactg ggtgcggctg gcggtctcgc gcgcgcaggc cacgggcgcg
     3901 ccggcggtgt tctggctcga cgagacgcgc gcccacgacg cccagctgct ggagaaggtg
     3961 cggccggcgc tcgaggagat cgacaccgag ggcgcccaga tcgagatcaa gccggtggcc
     4021 gaggccacgc gcttcacgct cgagcgggcg cgcgcggggg aggacacgat ctcggtgacc
     4081 gggaacgtcc tgcgtgacta cctgaccgac ctgttcccga tcctcgagct gggcaccagc
     4141 gcgaagatgc tctcgatcgt cccgctgatg ggcgggggcg gcctgttcga gaccggcgcc
     4201 ggcggctcgg cgcccaagca cgtccagcag ttcgtcaagg agaaccacct gcgctgggac
     4261 tcgctcggcg agttcctggc gctggccgtg tcgttcgagt tcctcgcgga caagaccgac
     4321 aacgcgcgcg cccgggtgct gggcgcgacg ctcgaccgcg ccaccggcag gctgctggag
     4381 gagggccgct cgccgctgcg caaggccggc gagctggaca accgcggcag ccacgcctac
     4441 ctggcggtgt actgggcggc cgagctggcc gcgcagaccg acgacgccga ccttgcggag
     4501 gccgtggcgc cgctggcgga gcgcctggcg gccgacgagg agaagatcgt cgaggagctg
     4561 aacggggtcc agggcgagcc cgtcgacatc ggcggctact tccacccgga caccgacaag
     4621 gtcgtcgccg cgatgcggcc cagccagacg ctgaacgccg cgctggacga cctcgacgcg
     4681 taggatcgcg cccatgactt cccccgttcg cgtagcggtc accggtgctg ccggccagat
     4741 cggctacgcc cttctcttcc gtatcgcttc cggccagctg ctgggccccg acacgccggt
     4801 gcacctgtcg ctgctggaga tccccgatgc cgtcaaggcc gccgagggca cggcgatgga
     4861 gctggatgac tccgccttcc cgctgctggc cggcatcgac atctccgacg acgccaagac
     4921 cgcgttcgac ggcgtgaacg tggcgctgct cgtgggcgcc cgcccgcgca ccaagggcat
     4981 ggagcgcgcc gacctgctgg aggccaacgg cggcatcttc aagccccagg gcgaggccct
     5041 ggccgccggc gccgccgacg acctgaaggt gctggtggtg ggcaaccccg ccaacaccaa
     5101 cgcgctgatc gcccagcaga acgccgacgg cgtgcccgcc gagcgcttca ccgccatgac
     5161 ccgcctggac cagaaccggg cgatgaccca gctggccaag aaggccggcg ctccggtcgg
     5221 cgaggtcacc aacctggccg tgtggggcaa ccactcgccg tccatgtacc cggacgtgtt
     5281 caacgccaag gtcggcggct ccgacgcatg ggaggccgcg ggcaaggacg agtcctggct
     5341 caaggacgac ttcatcccca ccgtcggcaa gcgcggcgcg gccatcatcg aggcgcgcgg
     5401 cgcctcctcg gcggcctcgg cggccaacgc ggccatcgac cacgtctacg actgggtcaa
     5461 cggcaccgcc gacggcgact tcgtgtccat ggcggtcccg tccgacggct cctacgacgt
     5521 gcccgagggc atcatctcct cgttccccgt gaccacctcg ggcggctcct acgagatcgt
     5581 ccagggcctg gacatcaacg agacctcgcg cggctacatc gacgccaccg cgcaggagct
     5641 cgtcgaggag cgcgaagccg tccagaagct cggcctcgtc tcctagcgcc tgaccccgtc
     5701 cggccttagg ctggacgggg ccttggcccg ggccccgaca ggagcggctg cccagcccgg
     5761 cggcacgctg ccgggcgccg tcgcggtgct cgcgctcgtc ccgctggccg gcttcgtgct
     5821 gttgctggcg gcgccctcgg ccgatgtgca gtgggaggac caccgggcgc acttctggct
     5881 cgtggttctc accgccctgg ccggcgcgtc gttcgcctac gccaccggcg agaccgcgcg
     5941 ccgccgcggc gactcgcgcc tgttcctgat ctacctggcg ttcctggccg ggggcggctt
     6001 cctggccgtg cacgcgctgg ccacccccgg cgtcctgctg gacggtccca acgccgggtt
     6061 caccctggcc acgccgatcg gcctgctggc cgcggcggcg ctggccgcgc tgtccacccg
     6121 cgatccgcag ccggccgccg gctcgccggc gctgcgggcg ctccagctcg ggctgatcgc
     6181 ggtcatggcc gtctgggtgg cggcctcgct gagcgaggtc c
//
LOCUS       DASUPW010000045         3723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1672319, whole genome shotgun sequence.
ACCESSION   DASUPW010000045 DASUPW010000000
VERSION     DASUPW010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672319"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /gene="rpsB"
                     /locus_tag="VFY44_01025"
     CDS             complement(<1..180)
                     /gene="rpsB"
                     /locus_tag="VFY44_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX5909041.1"
                     /translation="MAQVTLRELLEAGVHFGHQTRRWNPKMRPYLYGSRNGTHIIDLD
                     QTVQLFRRAFNVLADT"
     gene            complement(363..1256)
                     /locus_tag="VFY44_01030"
     CDS             complement(363..1256)
                     /locus_tag="VFY44_01030"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX5909042.1"
                     /translation="MRLDAYLVDRGLMPTRGAAQRAIEQGRVTVDGRTRSKSHKVAPG
                     EDVQVGPEPEQAPEAVPEVTFGVAYEDDELLVVDKPAGVVVHPGAGNEHGTLVQALAG
                     RVAGGSDPERPGVVHRLDRDTSGLLVVARTEAAHAELSRMIREREVTRRYLALVDGRP
                     PAASGTIDAPLGRDRKRRTVMSSDTDKPREAITHFELLEALPRTTLLDVKLQTGRTHQ
                     IRAHMQAIGHPVCGDPRYGGSAVGRRLGLERQFLHANLLMFRHPTTGEEVRCESKPPA
                     DLRRALDAARREPVSGGPDGH"
     gene            complement(1246..1704)
                     /gene="lspA"
                     /locus_tag="VFY44_01035"
     CDS             complement(1246..1704)
                     /gene="lspA"
                     /locus_tag="VFY44_01035"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX5909043.1"
                     /translation="MTRAQGWARAAVTVLAVLALDQVTKLLADGRIARGDSINVFFGL
                     DLTNTRNRGVAFGFLEGKGGVVGLLIAVALAALVVWFALNAGREWLWLPVGMLLGGAI
                     GNLIDRAREGAVIDFIDPIAWPAFNVADMCIVIGVLALLYVMEGPARREA"
     gene            complement(1709..2011)
                     /locus_tag="VFY44_01040"
     CDS             complement(1709..2011)
                     /locus_tag="VFY44_01040"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX5909044.1"
                     /translation="MTLLALTRGDVADYVGTLILVYTILMIVWVILSWIPRMPYHPVL
                     SAIVGFVNDVVNPYVGLFRRFIPPLKMGPGALDLSPIVAFLVLYIVGGIVVGLIRG"
     gene            complement(2008..2763)
                     /locus_tag="VFY44_01045"
     CDS             complement(2008..2763)
                     /locus_tag="VFY44_01045"
                     /inference="COORDINATES: protein motif:HMM:NF026098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX5909045.1"
                     /translation="MKLGFIGAGSMATGLARGLAEPALVFDPDAERAERLAAEIGGEV
                     ARSNADLAEQADVVVLGHKPDKLEEVADQVDGAADALASIVAATRIDQLEAAYPGVPV
                     YRLVPNIPVEVGRGVFGYARGSHAADGPEDELLQMLGRCGTVMELDEPLMEPAMAVMS
                     CGPAFMALVVQSLADAGERHGLDTQTAARLVVETMAGTAAYLDANDLDAAELRHRVAT
                     PGGVTERGLAVLEDRGLPAALGAAVDLVVETAR"
     gene            complement(2778..3386)
                     /locus_tag="VFY44_01050"
     CDS             complement(2778..3386)
                     /locus_tag="VFY44_01050"
                     /inference="COORDINATES: protein motif:HMM:NF016363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEX5909046.1"
                     /translation="MALSDTWHRALVYFGLAEEEDPRYEAVPDRDRERDRERARDRGP
                     ATDPGYEPEAELEDRYRERPNVRRLQRRRRDEFDDIFADDSPRETGRETRVLRSVGNG
                     GNGGSAAAMDQVHLVIPKAFNDAQEIANQFKDGIPVILNLQGIDADLSKRLIDFSSGL
                     TYALDGGMQRIADKVFLLTPRNVEVSAEERARLIEKGFFNQS"
     gene            3527..>3723
                     /locus_tag="VFY44_01055"
     CDS             3527..>3723
                     /locus_tag="VFY44_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5909047.1"
                     /translation="MCLHGLTATRRYVLQGSRMLARKGGRLVVGYDARGHGESSPAPD
                     GLYGYPELVADLEGVLDELGI"
BASE COUNT          528 a         1362 c         1293 g          540 t
ORIGIN      
        1 cgtgtcggcg aggacgttga acgcacgacg gaacagctgc accgtctggt cgaggtcgat
       61 gatgtgcgtg ccgttgcgcg agccgtagag atacggccgc atcttggggt tccagcggcg
      121 cgtctggtgg ccgaagtgca ccccggcctc cagcagctcc ctgagtgtta cctgagccaa
      181 ctgaatcgtt tcctttggtt gtgatgctgg ggaaaccggc ggagatgacg cggacccgga
      241 ctggttgtcc gggcaaggcc cacgcaccga cccaccgggg taggtgactt ggatgaacgg
      301 cagaggatag agccacgaac gaaaacgagg cggctcccgt agggaaccgc cccgcctcga
      361 tatcagtgcc cgtctggccc tccggacact ggctcccgcc tcgctgcgtc gagtgctcgg
      421 cgcagatcgg cgggtggttt ggactcgcac cggacttctt cccccgtggt cgggtgccga
      481 aacatgagca ggttcgcgtg caggaattgg cgctccagcc ccagccgccg gcccaccgcg
      541 gaacccccgt agcgggggtc gccgcacacc ggatggccga tcgcctgcat gtgcgcgcgg
      601 atctggtgcg tgcgccccgt ctgcagcttc acgtccagca gcgtcgtccg cggcagggcc
      661 tccaggagct cgaagtgggt gatggcctcg cgcggcttgt ccgtgtccga ggacatcacg
      721 gtccggcgct tgcggtcacg gcccaggggc gcgtcgatgg tgcccgaggc ggccggcggc
      781 cggccgtcca ccagcgccag gtagcggcgg gtgacctcgc gctcacggat catccgcgac
      841 agctcggcgt gcgcggcctc cgtgcgggcc accaccagca gccccgaggt gtcacggtcc
      901 aggcggtgga cgacgcccgg ccgctccggg tcggagccgc ccgcgaccct gcccgccagg
      961 gcctggacca gcgtgccgtg ctcgttgccc gcacccgggt gcacgaccac gcccgcgggc
     1021 ttgtccacca ccagcagctc gtcgtcctcg tacgccacgc cgaacgtgac ctcgggcacg
     1081 gcctcggggg cctgctcggg ctccgggccg acctgcacgt cctcgcccgg cgcgaccttg
     1141 tgggacttcg accgggtccg cccgtcgacg gtcacgcgcc cctgctcgat cgcccgctgc
     1201 gccgcgcccc gcgtgggcat cagcccccgg tcgaccaggt aggcgtcaag cctcacgccg
     1261 ggccggcccc tccatcacgt acagcagcgc aagcaccccg atcacgatgc acatgtcggc
     1321 cacgttgaac gcgggccagg cgatcgggtc gatgaagtcg atcaccgccc cctcgcgggc
     1381 gcggtcgatg aggttgccga tcgccccgcc cagcagcatc cccacgggca gccagagcca
     1441 ctcacggccg gcgttcagcg cgaaccacac caccagcgcc gccagcgcga cggcgatcag
     1501 cagccccacc acgccgccct tgccctccag gaatccgaag gccacgccgc ggttgcgcgt
     1561 gttggtcagg tccaggccga agaagacgtt gatgctgtcg ccgcgcgcga tccgcccgtc
     1621 ggccagcagc ttggtgacct ggtcgagggc cagcaccgcc agcaccgtca ccgcggcgcg
     1681 ggcccagccc tgggcgcgcg tcaccgggtc agccccggat cagcccgacc acgatgccgc
     1741 cgacgatgta cagcaccagg aacgcgacga tcgggctcag gtccagcgct ccgggaccca
     1801 tcttcagcgg cgggatgaag cggcggaaca ggccgacgta ggggttcacg acgtcgttca
     1861 cgaagcccac gatggccgac aggaccgggt ggtagggcat ccgcgggatc cagctcagga
     1921 tcacccagac gatcatcagg atcgtgtaga ccaggatcag cgtgcccacg tagtcggcca
     1981 cgtcgccccg ggtcagcgcc agcagggtca tcgcgccgtc tccaccacca ggtcgacggc
     2041 ggcgcccagc gccgcgggca ggccgcggtc ctcgagcacg gccaggccgc gctcggtcac
     2101 gccgcccggg gtggccacgc ggtggcgcag ctcggcggcg tccaggtcgt tcgcgtccag
     2161 gtaggccgcg gtgccggcca tggtctccac gaccaggcgg gcggccgtct gcgtgtccag
     2221 gccgtgacgc tcgccggcgt cggccagcga ctgcaccacc agggccatga acgcggggcc
     2281 gcagctcatg accgccatcg cgggctccat cagcggctcg tccagctcca tgaccgtgcc
     2341 gcaccggccc agcatctgca gtagctcgtc ctcggggccg tcggccgcat ggctgccgcg
     2401 cgcgtagccg aagacgccac ggccgacctc gaccgggatg ttgggcacca ggcggtagac
     2461 gggcacgccg gggtaggcgg cctcgagctg gtcgatgcgg gtcgcggcga cgatcgaggc
     2521 cagcgcgtcc gcggcgccgt cgacctggtc ggcgacctcc tcgagcttgt ccggcttgtg
     2581 gcccaggacc accacgtcgg cctgctcggc caggtccgcg ttggagcggg ccacctcgcc
     2641 accgatctcg gcggccaggc gctcggcgcg ctcggcgtcg gggtcgaaca cgagtgcggg
     2701 ctcggccagg ccacgggcca ggcccgtcgc catgcttccc gcaccgatga acccgagctt
     2761 cacccgcggg aagttaacta gctctggttg aagaagccct tctcgatcag acgagcgcgc
     2821 tcttccgccg atacctcgac gttgcgcggg gtcagcagga agaccttgtc ggcgatgcgc
     2881 tgcatgccac cgtcgagcgc gtaggtcaac cctgacgaga agtcgatcag gcgcttggac
     2941 aggtcggcgt cgatgccctg gaggttcagg atcacgggga tgccgtcctt gaactggttg
     3001 gcgatctcct gggcgtcgtt gaacgccttg gggatcacca ggtgcacctg gtccatggcg
     3061 gccgccgagc cgccgttgcc gccattgccc accgagcgca ggacgcgggt ctcgcgaccg
     3121 gtctcgcgcg ggctgtcgtc ggcgaagatg tcgtcgaact cgtcgcgccg gcgccgctgg
     3181 aggcgacgca cgttcgggcg ctcgcggtag cggtcctcca actcggcctc gggctcgtag
     3241 ccggggtcgg tggcgggccc gcggtcacgg gcgcgctccc ggtcgcgttc gcggtccctg
     3301 tccgggacgg cctcgtagcg cgggtcttcc tcctcggcca acccgaagta gacgagcgct
     3361 ctatgccatg tgtcgctgag ggccatcggg atgaggtttc gcgcagcgtg cgtgaatccc
     3421 tgcgtgttgt cacattatcc agatccccga tccccacccc ttcacttgca cgcccacgcc
     3481 cggcctgacc ctggccggct cccgcgaggg cgacggcccg ccgctggtgt gcctgcacgg
     3541 cctgaccgcc acccggcggt acgtgctcca gggctcgcgc atgctggccc gcaagggcgg
     3601 gcggctcgtc gtgggctacg acgcccgcgg gcacggtgag tcctcccccg cccccgacgg
     3661 cctctacggc taccccgagc tggtggcaga cctggagggc gtcctggacg agctcggcat
     3721 cga
//
LOCUS       DASUPW010000046         2422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1672632, whole genome shotgun sequence.
ACCESSION   DASUPW010000046 DASUPW010000000
VERSION     DASUPW010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672632"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2046)
                     /locus_tag="VFY44_01060"
     CDS             complement(<1..2046)
                     /locus_tag="VFY44_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX5909048.1"
                     /translation="MNTAELQDLINQASVFAEGEVVTRALSRDVVLPHVGRTAVLITL
                     DNGQDHTRPNTLGPRGLGEFNAALDTALARDDIAAILYTGKPFILAAGADLSAIAKIT
                     EREQALAIARIGHAVFDKLHTSAVPTFALINGLALGGGLEIALHAHYRTVSKAAAGIA
                     LPETFLGMFPGWGGAYLVPNLIGPERAVKLIIENPLNRNRMLDGAGAYALGLADALFD
                     GADFLERSLDWAGQVVAGTATVTRDPIDRGAAWDAALAEAREFVDAKVGGASPGPYRA
                     LDLMAAAKTADRPRAYAAEDEALADMIMGDELRASLYAFDLVRKRARKPAGAPDRSLA
                     RPVTKVGVIGAGLMASQLAMLFLRQLKVPIVITDVDQERVDKGLAAIHGEIDILVGKK
                     RLSTDGANRLRGLISGTTDLADFADCDFVIEAVFEDLSIKQQVFADLEKHVAPTCVLA
                     TNTSSLSVSEMAVDLAHPERVVGFHFFNPVAVLPLLEVVRAAKTDDATLATALAVAKD
                     LRKNAVLVKDAPAFVVNRVLLRMMGEVLATVDEGTPVKVADAALAPLGLPMPPFVLMQ
                     LVGLPIVLHVGETLHSAFGDRFPVSASLKALIEAKRTGIYDWTADGETYVSKETQALL
                     PVGDRPSTAAQVRERALTAVAEEIGLMLADGVVAAPMDVDLCLILGAGWPFHLGGITP
                     "
     gene            complement(2043..>2422)
                     /locus_tag="VFY44_01065"
     CDS             complement(2043..>2422)
                     /locus_tag="VFY44_01065"
                     /inference="COORDINATES: protein motif:HMM:NF014820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909049.1"
                     /translation="FAGVDPEAMGVGPIPATEKALAKAGLGIDDLGAFEINEAFAVQV
                     LAFLDHFGLAADDPRVNPYGGAIALGHPLASSGVRLMIHLARSFREHPEVRYGLTTMC
                     IGLGMGGTVVWENPHYTAEGVAA"
BASE COUNT          362 a          853 c          851 g          356 t
ORIGIN      
        1 cggggtgata ccgccgaggt ggaacggcca gcccgcgccc aggatcaggc agaggtcgac
       61 gtccatcggc gcggcgacca caccgtcggc cagcatcaac ccgatctcct cggccacggc
      121 ggtcaaggcc cgctcgcgga cctgcgcggc ggtgctcggt cgatccccga cgggcaacag
      181 ggcctgggtc tccttcgaca cgtaggtctc gccatccgcg gtccagtcgt agattccagt
      241 ccgcttggcc tcgatcagcg ctttcaggct ggccgacacc gggaaccggt caccgaacgc
      301 cgaatgcagg gtctcgccca catgcagcac gatcggcaga ccgaccagct gcatcagcac
      361 gaacggcggc atcggcagtc cgaggggggc cagggccgcg tcggcgacct tgaccggcgt
      421 gccctcgtcg accgtggcca gcacctcgcc catcatccgg agcagcaccc ggttgaccac
      481 gaaggcgggc gcgtccttca ccagaacggc gttcttccgc aggtccttgg ccacggccag
      541 tgcggtggcc agggtcgcgt cgtcagtctt cgcggcgcgg accacctcca gcagcggcag
      601 caccgcgacc gggttgaaga agtggaagcc gacgacccgc tcggggtggg cgaggtcgac
      661 cgccatctcc gacaccgaca gcgacgaggt gttggtggcc agcacgcagg tcggggcgac
      721 atgcttctcc aggtcggcga acacctgctg cttgatcgac aggtcctcga acacggcctc
      781 gatgacgaag tcgcagtcgg cgaagtcggc cagatcggtg gtcccggaga tcagcccgcg
      841 gagccggttg gccccgtcgg tggacagtcg cttcttgccc accaggatgt ctatctcccc
      901 gtggatcgcc gccagcccct tgtccacccg ctcctgatcc acgtcggtga tcacgatcgg
      961 caccttgagc tggcgcagga aaagcatggc gagctggctg gccatcagtc cggcgccgat
     1021 gacgcccacc ttggtcaccg gccgggccag tgagcggtcg ggagcgccgg caggcttgcg
     1081 ggcccgcttc cggaccaggt cgaaggcgta caggctggcc cgcagctcgt cgcccatgat
     1141 catgtcggcc agcgcctcgt cctccgcggc gtacgcgcgg ggccggtccg cggtcttggc
     1201 cgcggccatc aggtcgagcg cccggtacgg cccgggggac gcaccgccca ccttcgcgtc
     1261 gacgaactcc cgggcctcgg ccagcgcggc atcccaggct gcgccccgat cgatcgggtc
     1321 gcgggtgacg gttgcggtcc cggccaccac ttggccggcc cagtccagcg agcgctccag
     1381 gaagtcggcc ccgtcgaaca gtgcgtcggc cagccccagc gcgtacgcgc cggcgccgtc
     1441 gagcatccgg ttgcggttga gcgggttctc gatgatcagc ttcaccgcgc gttcggggcc
     1501 gatcaggttg ggcaccaggt acgccccacc ccagcccggg aacatgccca ggaaggtctc
     1561 gggaagcgcg atcccggcgg ccgccttcga cacggtgcgg tagtgggcgt gcagcgcgat
     1621 ctccaggcca ccgcccaggg ccagcccgtt gatcagggcg aaggtgggca cggcggaggt
     1681 gtgcagcttg tcgaagacgg catgcccaat ccgggcgatg gccagggcct gttcgcgctc
     1741 ggtgatcttg gcgatcgcgc tgagatcggc cccggcggcc aggatgaacg gcttgccggt
     1801 gtacaggatc gcggcgatgt cgtcccgggc gagggcggtg tccagcgcgg cgttgaactc
     1861 gcccagaccg cgcgggccca gcgtgtttgg ccgggtgtgg tcctggccgt tgtccaaggt
     1921 gatcagcacg gcggtgcggc cgacgtgggg gagcacgacg tcccggctca gcgcccgggt
     1981 caccacctcg ccctcggcga agaccgacgc ctgattgatc aggtcctgca gctcggcggt
     2041 gttcacgcgg ccactccttc cgcggtgtag tgcgggtttt cccagaccac ggtgccgccc
     2101 atgcccaggc cgatgcacat ggtggtcagg ccgtaccgga cctcggggtg ctcgcggaac
     2161 gagcgggcca ggtggatcat cagccggacg ccggagctgg ccagcgggtg gcccagggcg
     2221 atcgccccac cgtacgggtt gacccgcggg tcgtccgccg ccagcccgaa gtgatcgagg
     2281 aaggccagca cctgcacggc aaaggcctcg ttgatctcga aggcgccgag atcgtcgatg
     2341 cccagtccgg ccttggccag cgccttctcg gtggccggga tcggtccgac ccccatggcc
     2401 tcgggatcca ccccggcgaa gg
//
LOCUS       DASUPW010000047         4761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_980008, whole genome shotgun sequence.
ACCESSION   DASUPW010000047 DASUPW010000000
VERSION     DASUPW010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980008"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1349
                     /locus_tag="VFY44_01070"
     CDS             <1..1349
                     /locus_tag="VFY44_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5909050.1"
                     /translation="RGIHSLPWADPRPGAHTVRAGLMFLWDQVNAGVMCPISMTHSVI
                     PALREAPDLAAEWEPRLTSTEYGPRGEERSRGVGPASTGPGSPGAIAGMAMTEKQGGS
                     DVRANTTSAVQQSDGTYAITGHKWFCSYPPCDVFLTLAQAPAGLSCFLIEGADPGFQV
                     QRLKDKLGTRSLPSSEVEFRNVTGRLVGEEGRGVQTIIRMVNHTRLDCLIGSASGMRV
                     GTVQAIHHARHRSAFGNTLVDQPAMQNVLADLALESEAATATAMRIARGYDEDDAAFR
                     RVATAVSKYWVCKRAPLHAVEALECLGGNGYVEESGMPRLLRDSPLNSIWEGSGNVAA
                     LDVLRALAREQDGLPAFLGEVEQAAGADARLDAHLDGLKQRLAGLGEQDPQFAARRVV
                     EDMGLALQGSLLVRHAPAPVADAFCASRLAREGGHAWGTLPAGVDAKAIVDRAFAG"
     gene            1349..2215
                     /locus_tag="VFY44_01075"
     CDS             1349..2215
                     /locus_tag="VFY44_01075"
                     /inference="COORDINATES: protein motif:HMM:NF006128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX5909051.1"
                     /translation="MATLTYAVDGPVARITLDRPERGNGITMAMPRELADAVEQADLD
                     PAVHAIALSGNGKGFCGGYDLVDAAEGQMTGIGEPGSPLDPMVQAKNHDPSGTWDPMV
                     DWQMMRRNVRGFMSLFKADKPVLCKVHGFCVAGGTDMALCSDLLVMAADARIGYPPAR
                     VWGSPTTSMWLARLGPQRAKRMLLTGDLITGAQAAEWGLAIEAPEPAELDDRFEELLR
                     RVAALPVNQLVMMKLLVNQQLEAQGLGPTQVVGTLLDGIARHTPEGFAFQQKAMTEGW
                     QAAVAERDGPFA"
     gene            2291..2923
                     /locus_tag="VFY44_01080"
     CDS             2291..2923
                     /locus_tag="VFY44_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX5909052.1"
                     /translation="MSSSRRGIATAFATVAMLCAAVPAHANVPHTVQPGETLWSVAAA
                     NGLSTSSLAAANGLSADAGLIAGNTITIPSPGTAPAAAPGPASTAPQGTGASSSLVSA
                     VGWQESGHNNSMVSSAGAMGIMQVMPGTWDWVEQNLAGRQLDPASRQDNLTAGTLYLN
                     HLIARAGGDEATAIAGYYQGPESVRRIGMLPETQRYVANVQALRGRFGGR"
     gene            complement(2927..3343)
                     /locus_tag="VFY44_01085"
     CDS             complement(2927..3343)
                     /locus_tag="VFY44_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5909053.1"
                     /translation="MSETILVCVTPRVDEPWVPHTAAQLAAEMGAKAVILSVEEVESQ
                     RFEALPRKETRASAAEAAERAAERLREAGAEPADVLVRSGRPVETVLAVAEEVDADMI
                     LVGSSPRRPIAQKLLGSVALELVQRSPRNVMVISEP"
     gene            complement(3340..4692)
                     /locus_tag="VFY44_01090"
     CDS             complement(3340..4692)
                     /locus_tag="VFY44_01090"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX5909054.1"
                     /translation="MADAQTTPAETPLKRAIGAKLLLVFVIGDVLGAGIYALVGEVSA
                     EVGGAIWTAFTAALVLAGLTAFAYAELVTKYPMAAGSALYVNRAYDKPFFTFMVAFAV
                     MCSGIASAATLSRAFAGDYFQEFLTVNLDVVALAFIVVVAAVNLRGISESVKVNVALT
                     AVEVSGLLLIVVIGVAALFQGDAEFSRNFEFREGESVVAVLVGGTSLAFYALIGFEDA
                     VNVAEETKDPSRAFPRALFLGLAVAGVIYLLVTMTASMVVSTDVLAKSDGALLEVVRE
                     GPLGIPTKLFSFIALLAVANGALINMIMASRLVYGMSRQGIVPRAFSRTLQARQTPHI
                     AIFFTTVLAAVLILSGDLESLADTTVILLLAVFVIVNITVLVLRRDSVAHDHFTAPTF
                     VPVLGGAVALYLLYDKFTDDSEVFVRAALLLALGAVLWAVNRLITGPPDPIDVEELAG
                     "
BASE COUNT          716 a         1759 c         1673 g          613 t
ORIGIN      
        1 agcgcggcat ccactcgctg ccgtgggccg acccccgccc cggcgcccac accgtccgcg
       61 ccggcctgat gttcctgtgg gaccaggtca acgccggcgt gatgtgcccc atctccatga
      121 cccactcggt catccccgcg ctgcgcgagg cccccgacct ggccgccgag tgggagccgc
      181 ggctgacctc caccgagtac ggcccccggg gggaggagcg gagccggggc gttggcccgg
      241 cgagcaccgg acccggctct ccgggcgcca tcgcgggcat ggccatgacc gagaagcagg
      301 gtggctcgga cgtgcgcgcc aacaccacca gcgccgtcca gcagagcgac ggcacctacg
      361 cgatcaccgg ccacaagtgg ttctgctcct accccccgtg cgacgtgttc ctgaccctgg
      421 cccaggcgcc cgcggggctg tcgtgcttcc tgatcgaggg cgccgacccc gggttccagg
      481 tccagcggct caaggacaag ctgggcaccc gctcgctgcc ctcgtccgag gtcgagttcc
      541 gcaacgtgac cgggcgcctg gtcggcgagg agggccgcgg ggtgcagacc atcatccgga
      601 tggtcaacca cacgcggctg gactgcctga tcgggtcggc ctccgggatg cgcgtcggca
      661 ccgtccaggc cattcaccac gcccggcacc gctcggcgtt cggcaacacg ctggtcgacc
      721 agcccgcgat gcagaacgtg ctggccgacc tggcgctgga gtccgaggcc gccaccgcga
      781 ccgcgatgcg catcgcccgc ggctacgacg aggacgacgc ggccttccgc cgcgtggcca
      841 ccgcggtgtc caagtactgg gtgtgcaagc gcgcgccgct gcacgcggtc gaggcgctgg
      901 agtgcctggg cggcaacggc tacgtcgagg agtcgggcat gccgcggctg ctgcgcgact
      961 ccccgctgaa ctcgatctgg gagggctcgg gcaacgtcgc cgcgctggac gtgctgcgcg
     1021 ccctggcccg cgagcaggac ggactgcccg cgttcctggg cgaggtcgag caggccgcgg
     1081 gcgccgacgc ccgcctggac gcgcacctgg acgggctcaa gcagcggctg gccggcctgg
     1141 gcgagcagga cccccagttc gccgcccgcc gcgtggtcga ggacatgggc ctggctctgc
     1201 agggctcgct gctggtgcgc cacgcccccg cgccggtggc ggacgcgttc tgcgcctcgc
     1261 gcctggcccg cgagggcggc cacgcctggg gcacgctgcc cgcgggcgtc gacgccaagg
     1321 cgatcgtcga ccgcgccttc gccggctgat ggccacgctg acctacgccg tcgacggccc
     1381 ggtggcccgg atcacgctgg accggcccga gcgcggcaac ggcatcacca tggccatgcc
     1441 gcgcgagctg gcggacgccg tcgagcaggc cgacctggac cccgcggtgc acgccatcgc
     1501 gctgagcggc aacggcaagg gcttctgcgg cggctacgac ctggtcgacg cggccgaggg
     1561 ccagatgacg ggcatcggcg agcccgggtc gccgctggac cccatggtcc aggcgaagaa
     1621 ccacgacccg tcgggcacct gggacccgat ggtcgactgg cagatgatgc gccgcaacgt
     1681 gcgcggcttc atgtcgctgt tcaaggccga caagccggtg ctgtgcaagg tgcacggctt
     1741 ctgcgtggcc gggggcaccg acatggcgct gtgctcggac ctgctggtca tggccgcgga
     1801 cgcccgcatc ggctacccgc ctgcgcgcgt ctggggctcg cccaccacgt cgatgtggct
     1861 ggcccgcctg ggcccgcagc gggccaagcg gatgctgctc accggcgacc tgatcaccgg
     1921 cgcccaggcg gccgagtggg gcctggccat cgaggccccc gagccggcgg agctggacga
     1981 ccggttcgag gagctgctcc ggcgcgtcgc cgcgctgccc gtgaaccagc tggtgatgat
     2041 gaagctcctg gtcaaccagc agctcgaggc gcagggcctg ggccccacgc aggtggtggg
     2101 gacgctgctg gacggcatcg cgcgccacac cccggagggc ttcgccttcc agcagaaggc
     2161 gatgaccgag ggctggcagg ccgccgtggc cgagcgcgac ggcccgttcg cctgatgcac
     2221 gaaccgtttc aggctccgta actccacacg ggttcgcagg gaccgccccg cggaccggca
     2281 aggacgggcc atgagttcct cacgcagggg gatcgcgact gcgttcgcga ccgtggccat
     2341 gctgtgcgcc gccgtccccg cccacgcaaa cgtcccccac acggtgcaac ccggcgagac
     2401 cctgtggtcg gtcgccgcgg ccaacggcct gagcacgagc tcgctggcgg ccgccaacgg
     2461 gctgtccgcc gacgccggcc tgatcgccgg caacaccatc accatcccct cccccggcac
     2521 ggcgccggcc gcagcgccgg gtcccgcctc caccgcgccg cagggcacgg gcgcatcgtc
     2581 ctcgctggtc tccgccgtgg gctggcagga gtcggggcac aacaactcca tggtctcgtc
     2641 cgcgggcgcc atggggatca tgcaggtcat gcccggcacc tgggactggg tcgagcagaa
     2701 cctggccggg cgccagctgg accccgcgtc gcgccaggac aacctgaccg cgggcacgct
     2761 ctacctcaac catctgatcg cccgcgcggg cggcgacgag gccactgcca tcgccggcta
     2821 ctaccaggga ccggagtcgg tgcggcggat cggcatgctg cccgagaccc agcgctacgt
     2881 ggccaacgtg caggcgctgc gcggccgctt cggcggccgg tagccgctac ggctcgctga
     2941 tgaccatgac gttgcggggc gagcgttgca ccagctcgag cgcgacgctc cccagcagct
     3001 tctgggcgat cgggcgccgg ggggaggagc cgaccaggat catgtcggcg tcgacctcct
     3061 cggccacggc caggacggtc tcgaccggcc ggccgctgcg caccagcacg tccgccggct
     3121 cggccccggc ctcgcgcagc cgctcggcgg cgcgctcagc ggcctcggcg gcgctggccc
     3181 gggtctcctt gcggggaagc gcctcgaagc gctgactctc gacctcctcc accgacagga
     3241 tcaccgcctt cgcccccatc tcggcggcca gctgggccgc cgtgtgcggg acccagggct
     3301 cgtccacacg cggcgtcacg cacaccagga tcgtctcgct catcccgcca gctcctccac
     3361 gtcgatcggg tcgggcgggc ccgtgatcag ccggttgacc gcccagagaa cggcgccgag
     3421 cgccagcagc agcgccgcac gcacgaacac ctcgctgtcg tcggtgaact tgtcgtagag
     3481 caggtacagc gccacggcgc cgcccagcac cggcacgaac gtcggcgcgg tgaagtggtc
     3541 gtgggccacg ctgtcgcggc gcagcaccag gaccgtgatg ttcacgatca cgaacacggc
     3601 cagcagcagg atcacggtgg tgtcggccag cgactcgagg tcaccgctca ggatcaggac
     3661 cgcagccagc accgtggtga agaagatggc gatgtggggc gtctggcggg cctgcagggt
     3721 gcggctgaag gcccgcggga cgatgccctg ccgcgacatc ccgtagacca gccgcgaggc
     3781 catgatcatg ttgatcaggg ccccgttggc caccgccagc agcgcgatga acgagaacag
     3841 cttggtcggg atgcccagcg gcccctcacg caccacctcc agcagcgcgc cgtcggactt
     3901 ggccaggacg tcggtggaca ccaccatcga ggcggtcatg gtcaccagca ggtagatgac
     3961 gccggccacg gccaggccca ggaacagcgc ccgcgggaac gcgcgcgagg ggtccttggt
     4021 ctcctcggcc acgtttaccg cgtcctcgaa gccgatcagg gcgtagaagg ccagggacgt
     4081 gccgccgacc aggacggcca ccacgctctc cccctcccgg aactcgaagt tgcgcgagaa
     4141 ctcggcgtcg ccctggaaca gggcggccac gccgatgacc acgatcagca gcaggccgct
     4201 gacctcgacg gcggtgaggg ccacgttgac cttcaccgac tccgagatgc cgcgcaggtt
     4261 cacggcggcc acgaccacga tgaaggccag ggccaccacg tccaggttca cggtcaggaa
     4321 ctcctggaag tagtcgccgg cgaaggcgcg cgacagcgtc gccgcggacg cgattcccga
     4381 gcacatcacg gcgaacgcga ccatgaaggt gaagaagggc ttgtcgtagg cccgattgac
     4441 gtacagtgca gagcccgccg ccatggggta cttggtgacc agctcggcgt aggcgaacgc
     4501 cgtgaggccc gccagtacca gcgcggcggt gaacgcagtc cagatggcgc cgcccacctc
     4561 ggcactgacc tcgcccacca gtgcatagat gccggcgccc agcacgtcgc cgatcacgaa
     4621 gaccagcagc agcttggcgc cgatcgcccg tttgaggggc gtttcggcgg gggtggtttg
     4681 ggcatcggcc attgggggtc cgtacccatt catgtcccac cccacccctg ccgatgatcc
     4741 ataaggccct ttcggcgcgc a
//
LOCUS       DASUPW010000048         4166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_842130, whole genome shotgun sequence.
ACCESSION   DASUPW010000048 DASUPW010000000
VERSION     DASUPW010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842130"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VFY44_01095"
     CDS             complement(<1..367)
                     /locus_tag="VFY44_01095"
                     /inference="COORDINATES: protein motif:HMM:NF036723.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909055.1"
                     /translation="MEPVTALSHPDATHDVRNQAPPLQPFNVYEADTALQEALEREGG
                     GWGADRFADTGALAGSREALEHSERCERNEPVLKTHDRYGNRVDEIQLDPSWHWLMRE
                     GIERGIHSLPWADPRPGAHT"
     gene            complement(355..984)
                     /locus_tag="VFY44_01100"
     CDS             complement(355..984)
                     /locus_tag="VFY44_01100"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5909056.1"
                     /translation="MAYRRTPRVEARQAAARERIAAAAHDQVAEGGYASASVQAVAAR
                     AGVSVGTVYRHFPSKGELVAEVFRTAAERELAVVQQVGADDGRPAAERIAAGAEAFCR
                     RALAAPTLAYAQLAEPVDPAVESERLRLRRGYRDAFESVLRDGVDAGELGPHDTTTVA
                     AALVGALGEALVGPLAAGADGRHDALVATLVDFCLSSLPTTEGASAWSP"
     gene            complement(1028..2608)
                     /locus_tag="VFY44_01105"
     CDS             complement(1028..2608)
                     /locus_tag="VFY44_01105"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX5909057.1"
                     /translation="MPQQPLSRRHFVSGASALGAATIFAPQALAARGKRQPTLRGGKF
                     RQGVLSGDPSPTAITLWTKVSGVGGSGSVELEVARDRGFKRVVARKLIPTNGSIDHVV
                     KARVGNLKAHEQYFYRFSTRGENSPAGRFRTALPPDSRQPVRFAFFSCQDYSFGYFNA
                     HTSLAREDIDFVVNLGDYIYGEAYYAPTNPKGGVRIDRVGEAITVADYRAKYDLYRSE
                     SALRKMHAQFPMISIWDDHEVQDNYAGGAGPTGGLTPEKRFSLARKRAGYKAYFENMP
                     TFGVGNPKGNRIYKAARFGRNVDLVLMDQRQYRADQPCGDKQVGAPCAELNNPRPYLG
                     DRQMSFIKNRLAKTPATWKIMANQAMIMRTVYPGGNYIGFDPWQGYPQERTELLQHIK
                     NRKIDNVVFITGDIHTIIAGDVRLGDNDKTPVATEFVGGSITSPGLGEGGGDVVPGAN
                     PRNPKTPQAIIDLLYKSNPWVKNADPDHHGYGLVEAGSNGLKCSFRRVPGVQKRSGAR
                     LPLGPFTYRLAKGQKSIL"
     gene            2750..3877
                     /locus_tag="VFY44_01110"
     CDS             2750..3877
                     /locus_tag="VFY44_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909058.1"
                     /translation="MKKTLIAALAASLLAVPSADAAKRKVPATFSGVQLDGGAETDPP
                     AVQEAQFALMARSGVESARIVFSWRDMQPQKNGAFDFTRTDAQVGAAARHGIVVLPVM
                     LYAPDWARAFPRDGGSPPLTAPYQGFLRATIRRYGTGGSYWRENPAVPRRTIRYWQVW
                     NEPHFEGFWDAPAKSRYSYPRGYARLLSASNKTIHATDRRARTVTAGLFGAAWERLAD
                     LYRSGGVRKSFDVAAVHVYSNKPGNVLETVKRTNATMRRYKDGGNRVWVTETSFPASK
                     GQARPIRNQKQDTPASMARKLTDTYVLLARNAKKQRIDRVYWYTWASSYQQTQPSNFD
                     YAGLLKRTGPFSYTPQPALDSFRRVSQRLQGCAKTDAGACR"
     gene            complement(3874..>4166)
                     /locus_tag="VFY44_01115"
     CDS             complement(3874..>4166)
                     /locus_tag="VFY44_01115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5909059.1"
                     /translation="AMPSLHEGLGLPVLEAMAAGTPVVSSNRGALPETCGDAALVVDP
                     DDHEAFAAALVRAALDAGEHQRLAAAGRERAAGFTWRRTAERVDELLEPLLG"
BASE COUNT          564 a         1436 c         1567 g          599 t
ORIGIN      
        1 ccgtgtgggc gcccgggcgc gggtcggccc agggcagcga gtggatgccg cgctcgatgc
       61 cctcgcgcat cagccagtgc caggacgggt cgagctggat ctcgtcgacg cggttgccgt
      121 agcggtcgtg ggtcttgagc acgggctcgt tgcgctcgca gcgctcggag tgctcgagcg
      181 cctcgcgcga gccggccagc gcgccggtgt cggcgaaccg gtccgcgccc cacccgccgc
      241 cctcgcgctc aagcgcctcc tgcagcgcgg tgtcggcctc gtagacgttg aagggctgga
      301 gcggcggcgc ctggttgcgc acgtcgtggg tcgcgtcggg gtgggacagg gcggtcacgg
      361 gctccatgcg gaggctcctt cggtcgtggg aaggctggaa aggcagaagt cgacgagcgt
      421 ggccacgagg gcgtcgtggc ggccgtcggc gccggcggcc agcgggccga ccagcgcctc
      481 gccgagcgcg ccgaccaggg cggcggccac ggtggtggtg tcgtgggggc cgagctcgcc
      541 ggcgtcgacg ccgtcgcgca gcacggactc gaaggcgtcg cggtagccac ggcgcaggcg
      601 caggcgctcg ctctccacgg ccgggtcgac gggctcggcc agctgggcgt aggccagggt
      661 gggggcggcc agggcgcggc ggcagaacgc ctcggcgccc gcggcgatgc gctcggcggc
      721 gggccggccg tcgtcggcgc ccacctgctg gacgaccgcg agctcgcgtt cggcggcggt
      781 gcggaagacc tcggccacca gctcgccctt ggacgggaag tggcggtaca cggtgcccac
      841 ggagacgccg gcgcgggccg ccaccgcctg gaccgacgcc gacgcgtatc cgccctcggc
      901 cacctggtcg tgcgcggccg cggcgatgcg ctcgcgggcg gcggcctgac gggcctctac
      961 tcggggggta cggcggtagg ccacccaagg agtgaatcac gattcactcc ttggggcaac
     1021 cgcatcgtca gaggatcgac ttctgcccct tggccagccg gtaggtgaag gggccgagcg
     1081 gcagccgcgc cccggaccgc ttctggaccc cgggcacgcg ccggaagctg cacttcagcc
     1141 cgttcgatcc cgcctcgacc agcccgtagc cgtggtggtc ggggtcggcg ttcttgaccc
     1201 acgggttgct cttgtagagc aggtcgatga tggcctgcgg ggtcttgggg ttgcgcgggt
     1261 tggcgccggg cacgacgtcg ccgccgccct cgcccaggcc gggggaggtg atcgacccgc
     1321 ccacgaactc ggtggccacc ggcgtcttgt cgttgtcgcc gaggcggacg tcgccggcga
     1381 tgatcgtgtg gatgtcgccg gtgatgaaca cgacgttgtc gatcttgcgg ttcttgatgt
     1441 gctggagcag ctcggtgcgc tcctgcgggt agccctgcca cgggtcgaag ccgatgtagt
     1501 tgccaccggg gtagacggtc cgcatgatca tcgcctggtt ggccatgatc ttccacgtgg
     1561 ccggggtctt ggccaggcgg ttcttgatga agctcatctg gcggtcgccc aggtacggcc
     1621 gggggttgtt cagctccgcg cacggcgcgc cgacctgctt gtcgccacag ggctggtcgg
     1681 cgcggtactg gcgctggtcc atcagcacca ggtcgacgtt gcggccgaag cgcgcggcct
     1741 tgtagatgcg gttgcccttg gggttgccca ccccgaaggt cggcatgttc tcgaagtagg
     1801 ccttgtagcc cgcgcgcttg cgggcgaggc tgaaccgctt ctccggcgtg aggccgccgg
     1861 tggggccggc gccgccggcg tagttgtcct ggacctcgtg gtcgtcccag atcgagatca
     1921 tcgggaactg cgcgtgcatc ttgcgcagcg cggactcgct gcggtagagg tcgtacttgg
     1981 cccggtagtc ggccacggtg atggcctcgc caacgcggtc gatgcgcacg ccgcccttgg
     2041 gattggtggg cgcgtagtag gcctcgccgt agatgtagtc gcccaggttg accacgaagt
     2101 cgatgtcctc gcgggcaagc gacgtgtgcg cgttgaagta gccgaacgag tagtcctggc
     2161 acgagaagaa ggcgaaccgg accggctggc gcgagtcggg cgggagcgcc gtgcggaagc
     2221 gcccggcggg gctgttctcg cccctggtcg agaagcggta gaagtactgc tcgtgggcct
     2281 tgaggttgcc cacgcgcgcc ttcaccacgt ggtcgatcga tccgttggtg gggatgagct
     2341 tgcgcgcgac cacgcgcttg aagccgcggt cacgggccac ctccagctcc acgctgcccg
     2401 agccgccgac gccgctgacc ttggtccaaa gggtgatcgc ggtgggcgac gggtcgcccg
     2461 acaggacgcc ctggcggaac ttgccgcccc gcagcgtcgg ctggcgcttg ccgcgggccg
     2521 ccagggcctg cggggcgaag atggtggcgg cgccgagggc gctggcaccg ctgacgaaat
     2581 ggcggcgcga caggggctgc tggggcacgg tctcctccaa gtccggttgc tgcccggagg
     2641 ggttcgcccc gagcaaggag actctacgcc cgctggtggg atctggaaag ggagcgggcg
     2701 ttaagccggg cgcccgcaac cctgactacg ctgccgtgtc ttgccaccag tgaagaaaac
     2761 gctcatagcc gccctggccg cgtccctgct ggccgtgccg tccgccgacg cggccaagcg
     2821 caaggtcccc gccaccttca gcggcgtcca gctggacggc ggcgccgaga ccgacccgcc
     2881 ggccgtgcag gaggcgcagt tcgccctgat ggcccgctcc ggcgtggaga gcgcgcggat
     2941 cgtgttcagc tggcgcgaca tgcagccgca gaagaacggc gccttcgact tcacccgcac
     3001 cgacgcccag gtcggcgccg cggcccgcca cgggatcgtg gtgctgccgg tgatgctcta
     3061 cgccccggac tgggcgcggg ccttcccccg cgacggcggc tcgccgccgc tgacggcgcc
     3121 ctaccagggc ttcctgcgcg ccaccatccg gcgctacggg acgggcggct cgtactggcg
     3181 cgagaacccg gcggtgccgc gcaggacgat ccggtactgg caggtctgga acgagccgca
     3241 cttcgagggc ttctgggacg cgccggccaa gagccggtac tcctacccgc gcgggtatgc
     3301 gcgcctgctg agcgcgtcga acaagacgat ccacgcgacc gaccgccgcg cccgcacggt
     3361 cacggccggc ctgttcggcg cggcctggga acggttggcc gacctgtacc gcagcggggg
     3421 cgtgcgcaag agcttcgacg tcgcggcggt gcacgtgtac tcgaacaagc cgggcaacgt
     3481 gctggagacc gtgaagcgca cgaacgcgac catgcgcagg tacaaggacg gcgggaatcg
     3541 cgtgtgggtc accgagacct cgttcccggc ctccaagggc caggccaggc cgatccgcaa
     3601 ccagaagcag gacacgccgg cgagcatggc gcgcaagctg accgacacct acgtgctgct
     3661 cgcgcgcaac gcgaagaagc agcgcatcga tcgcgtgtac tggtacacgt gggcgagctc
     3721 gtaccagcag acccagccca gcaacttcga ctacgcgggc ctgctgaagc gcacggggcc
     3781 gttcagctac acgccccagc ccgcgctgga ctccttccgg cgggtctcgc agcggctcca
     3841 gggctgcgcg aagaccgacg ccggcgcctg ccgctagccc aggagcggtt ccagcagctc
     3901 gtcgacccgc tcggccgtgc ggcgccaggt gaagccggcg gcccgctcgc ggcccgcggc
     3961 ggccagacgc tggtgctcgc cggcgtcgag cgcggcccgc accagcgcgg ccgcgaacgc
     4021 ctcgtggtcg tcgggatcga ccaccagggc cgcgtcgcca caggtctcgg gcagcgcgcc
     4081 ccggttcgag ctgaccacgg gcgtgcccgc cgccatggcc tcgagcaccg gcagccccag
     4141 gccctcgtgc agtgacggca tggcca
//
LOCUS       DASUPW010000049         4172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_149286, whole genome shotgun sequence.
ACCESSION   DASUPW010000049 DASUPW010000000
VERSION     DASUPW010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149286"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1765)
                     /locus_tag="VFY44_01120"
     CDS             complement(104..1765)
                     /locus_tag="VFY44_01120"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX5909060.1"
                     /translation="MELDGAKLPDGSRLEADVAIVGGGPAGIVTALELAEAGLDVLLV
                     ESGGPRLDQDTQHLGEREPAGDGLHLPEASSARRQLGGNTVTWGGRCVPFDPVDFDAR
                     DLTGSARWPIGYADLLPHFDQACRYLDCGLGGFDAQGVPGLAGKTVVPGLPDGDVRTS
                     SLERWSLPTNFGRHYGDRLRRSPRIRVLLRQTCTRVVLDDRGQAVAELETKALDGRGA
                     TIAARRYVLSAGGLESTRLLMVSGIGDHSGHLGRWYMAHPQGTIAGARFSTPPGDTVY
                     AHERDPDGVYVRRRFGFSREFTRRHDLPSFAAWLVNPALADPRHRSGMLSLVYLALAS
                     PAWPLFASQSLREDHVAEAGTAALLPHVRNLARDPAGAMRFATGFGYRRYLRRGRRVP
                     GFSTYSPDNRYPLEYQSEHLPHRESHVTLGSGTDRLGVPRLRTHLHYGQADVDGVLDA
                     HRRIDRYLRDHGVGRLEYDSADPEARVREQLFAGCHQAGTTRMSERPEDGVVDTDLAV
                     HGVGNLHVASSSVFVTSSHANPTFMILVLALRLAGHLRGQLCSRV"
     gene            complement(1854..2777)
                     /locus_tag="VFY44_01125"
     CDS             complement(1854..2777)
                     /locus_tag="VFY44_01125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HEX5909061.1"
                     /translation="MQWIALLIALAVLLAIDLLIVRGRDGAMSIRAASVWSVAWIAIS
                     LIFAGVLYVAGSSGQAESYLAGYLVEKSLSLDNVFVFLIVFQAFSIAEAARPKLLTYG
                     IVLALVLRAIFIFAGATALEHASWLSFPFAALLAWTGWRLWKHRHEHGGEEQLVNGVK
                     DRLNVAEGDHGAKLIVRQDGKRALTLGGAALVAIAVVDIIFAVDSVPAILAITTDTYV
                     VFAANAFALLGLRPLFFLVADLVERLYYLKAALAILLMFIAGKMIAGELVGKVGPAIS
                     LAGVALILGAGVVASLLRKRSEEPAPEHDPA"
     gene            3087..>4172
                     /locus_tag="VFY44_01130"
     CDS             3087..>4172
                     /locus_tag="VFY44_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5909062.1"
                     /translation="MSSAVIDARGVTVTYEPRVVLDAVDLRIGRDSRIALIGPNGSGK
                     STLLRVLAGTEPSWSGSVYRHGEIGHLPQMAGHQGRETAREVILERIGVAAASREVDR
                     LAALLADGDLDAVDDHATALERWLALGGGDAEPRLEAAAAEAGLEPALLDRPLATLSG
                     GQAARAGLAALRTARFDALLLDEPTNHLDADGLAFLSRVLDEAAGGVVLVSHDRGVLS
                     GFADELVELDARTGRATHYGGGWDAYERERDAARERERAEYEHAVARRTHVEEAEREV
                     RRRAAASARNGAKAARDGDKHGREWVKMRAEEAQSRARKIGTRAARVHGPDKPRAAPR
                     LRLSLSRGERRGGPLVALEGAVLRRGSF"
BASE COUNT          580 a         1475 c         1589 g          528 t
ORIGIN      
        1 atgagctcga actcggtgac ggcgccggcc agcggctcgc cgcccgggaa ggtgaccgac
       61 acgtcccagg tgcccacgag cgggccgagc tggtccaggg ggctcacacc cgcgaacata
      121 gctggccgcg caggtgcccg gccagccgca gcgccagcac caggatcatg aaggtcgggt
      181 tggcgtggct ggaggtcacg aagacggagc tgctggccac gtgcaggttc ccgacgccgt
      241 ggacggccag gtcggtgtcc acgaccccgt cctcgggtcg ctcggacatg cgcgtggtgc
      301 cggcctggtg gcagcccgcg aagagctgct cgcggacgcg cgcctcgggg tcggccgagt
      361 cgtactccag tcgcccgacc ccgtggtcgc gcaggtaccg gtcgatgcgc cggtgggcgt
      421 ccaggacacc gtccacgtcg gcctggccgt agtggaggtg ggtgcgcagc cgcggcaccc
      481 ccagccggtc ggtcccggag cccagggtca cgtggctctc gcggtggggc aggtgctcgc
      541 tctggtactc cagcgggtag cggttgtcgg ggctgtaggt ggagaagccc gggaccctgc
      601 gaccccgtcg cagatagcgc cggtagccga agccggtggc gaacctcatc gcgccggcgg
      661 ggtcacgggc caggttgcgg acgtggggga gcagcgcggc ggtgccggcc tcggccacgt
      721 ggtcctcgcg gaggctctgg gacgcgaaca ggggccaggc gggggaggcc agggccaggt
      781 agaccagcga cagcatcccg ctgcggtggc gcgggtcggc cagcgccggg ttcaccagcc
      841 aggccgcgaa gctgggcagg tcgtgccggc gcgtgaactc gcgcgagaac ccgaaccgcc
      901 ggcgcacgta caccccgtcg gggtcccgct cgtgggcgta gacggtgtcg ccgggagggg
      961 tcgagaagcg ggcaccggcg atggtgccct gcgggtgggc catgtaccac cgccccaggt
     1021 ggccggagtg gtcgccgatc ccggacacca tcagcagacg ggtgctctcc aggccgccgg
     1081 cggacaggac gtaccggcgc gcggcgatcg tggcgccgcg cccgtccagc gccttcgtct
     1141 cgagctcggc gaccgcctgc ccccggtcgt ccagcaccac cctggtgcag gtctggcgca
     1201 acagcacccg gatccgcggc gagcggcgca ggcggtcgcc gtagtggcgg ccgaagttcg
     1261 tgggcagcga ccaccgctcc agcgacgacg tccgcacgtc gccgtcggga agaccgggca
     1321 cgacggtctt cccggccagc ccgggcacac cctgggcatc gaatccgccc aggccgcagt
     1381 ccaggtagcg gcacgcctgg tcgaagtggg gaaggaggtc ggcgtacccg atcggccacc
     1441 gggcgctgcc ggtcaggtcc cgggcgtcga agtcgaccgg gtcgaacggc acgcagcgcc
     1501 cgccccaggt gaccgtgttg ccgcccagtt gccggcgcgc cgaggaggcc tcgggaaggt
     1561 gcaggccgtc gccggccggc tcccgttcgc ccaggtgctg ggtgtcctgg tcgaggcggg
     1621 gcccgccgct ctcgaccagc agcacgtcga gcccggcctc cgccagctcg agtgcggtca
     1681 cgatgcccgc ggggccgccg ccgacgatgg ccacgtcggc ctccagccgg ctgccgtcgg
     1741 gcagcttggc gccgtctagc tccatcccgg cgtcgcgggt gcgcagcgga tgggtgctgg
     1801 gtgggctgcc atggcgtcgc gggtgcgccg gccggcccgc ccgcgacgcg atcctatgcc
     1861 gggtcgtgct cgggtgccgg ttcctcgctc cgcttgcgca gcagggaagc caccacaccg
     1921 gcgcccagga tcagcgcgac gcccgcgagg gagatcgcgg gaccgacctt gcccaccagc
     1981 tcgccggcga tcatcttgcc ggcgatgaac atcagcagga tcgccagcgc ggccttgagg
     2041 tagtacaggc gctcgaccag gtcggccacc aggaagaaca gcggccgcag gcccagcaac
     2101 gcgaacgcgt tggcggcgaa caccacgtag gtgtcggtgg tgatggccag gatggccggc
     2161 accgagtcga cggcgaagat gatgtccacc accgcgatcg ccacgagggc ggcgccgccc
     2221 agggtcaggg cgcgcttgcc gtcctggcgc acgatcagct tggcgccgtg gtcgccctcg
     2281 gccacgttga gccggtcctt gacgccgttg accagctgct cctcgccgcc gtgctcgtgg
     2341 cggtgcttcc agagccgcca gccggtccag gccagcaggg cggcgaacgg gaacgagagc
     2401 cacgacgcgt gctccagggc ggtggcgccg gcgaagatga atatggcccg caggaccagc
     2461 gccaggacga tgccgtaggt cagcagcttg ggccgcgcgg cctcggcgat cgagaacgcc
     2521 tggaacacga tcaggaacac gaagacgttg tccagcgaca gcgacttctc gaccaggtag
     2581 ccggccaggt agctctcggc ctgtccggag gagccggcca cgtagagcac cccggcgaat
     2641 atcagcgaga tggcgatcca cgccacgctc cagacggacg cggcgcggat ggacatggcg
     2701 ccgtcgcggc cgcggacgat caacagatcg atggccagca gcacggcgag ggcgatgaga
     2761 agtgcgatcc actgcatggg gtgagacctc cgcgagtcgt cgaagaacgt tcgcgaaggt
     2821 ttctccttcc ccgtgctggg gcgctggtgc cgggcgggct atatggggcc acaccgtgct
     2881 gacggcagtc agcctccggg atacttccct tcggtccgcg ggactgtagc gacccgtgcc
     2941 gccgccaggc cccttgatcc ccgtggtacc ttggatttcg gcctgcttgt gaggcctgcc
     3001 tatgcgcttc ttcgactgac aaccgccacg cccgccgcgc tcgcggttgt cacatcgtct
     3061 gccgttccgt cgtcgaagga gcacccttgt cttccgccgt aatcgacgcc cgaggcgtca
     3121 ccgtcaccta cgagccgcgt gtcgtgctgg acgcggtcga cctccgcatc ggccgtgaca
     3181 gccggatcgc gctgatcggc ccgaacgggt ccggcaagtc cacgctgctg cgcgtcctgg
     3241 cagggaccga gccctcctgg agcggctcgg tctaccgcca cggggagatc ggccacctgc
     3301 cccagatggc cggccaccag ggccgggaga ccgcgcgcga ggtgatcctg gagcgcatcg
     3361 gcgtggccgc cgcctcgcgc gaggtcgacc gcctggccgc gctgctggcg gacggcgacc
     3421 tggacgccgt ggacgaccac gccaccgcgc tggagcggtg gctggccctg ggtggcggcg
     3481 acgcggagcc caggctggag gccgcggccg ccgaggcggg cctggagccc gctctgctgg
     3541 atcgcccgct ggcgaccctg tcgggcgggc aggcggcccg cgccgggctg gcggcgctgc
     3601 gcaccgcgcg cttcgacgcg ctgctcctgg acgagcccac caaccacctg gacgccgacg
     3661 gcctggcctt cctttcgcgg gtgctggacg aggccgccgg cggggtggtg ctggtctcac
     3721 acgaccgcgg cgtgctgtcg gggttcgccg acgagctggt cgagctggac gcgcgcaccg
     3781 gccgtgccac ccactacggc ggcggctggg acgcgtacga gcgcgagcgc gatgcggccc
     3841 gcgagcgcga gcgggccgag tacgagcacg cggtggcccg gcgcacgcac gtcgaggagg
     3901 ccgagcgcga ggtgcgccgt cgggccgcgg ccagcgcgcg caacggcgcg aaggccgccc
     3961 gcgacggcga caagcacggc cgcgagtggg tgaagatgcg cgccgaggag gcccagtcgc
     4021 gggcacgcaa gatcgggacg cgcgcggccc gcgtgcacgg gcccgacaag ccgcgggcgg
     4081 ccccgcgcct gcgcctgtcg ctgagccgcg gcgagcgccg cggcggaccg ctggtggcgc
     4141 tcgagggcgc ggtcctgcgg cgagggagct tc
//
LOCUS       DASUPW010000050         3362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1534945, whole genome shotgun sequence.
ACCESSION   DASUPW010000050 DASUPW010000000
VERSION     DASUPW010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534945"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="VFY44_01135"
     CDS             <1..1185
                     /locus_tag="VFY44_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX5909063.1"
                     /translation="QLVESGGADLRTQAEGFIPGGATFKNITRHSAMGIPTVSLVFGN
                     STAGGAYVPGMSDYNIFVKERAKVFLGGPPLVKMATGEVSEDEELGGADMHARVSGLA
                     DYLANDEYDAIRLGREVVRRLNWRKKGPGPTESPDPPVHDPDELLGIVPPDLKAPFDP
                     REVIARVVDGSRYDEFKALYGPSLTTGWASIHGYPVGIIANVQGVLFSPEAQKAAQFI
                     QLANQKDVPLVFMHNTTGYMVGKDYEQGGIIKHGAQMINAVSNSKVPHLGVIMAASYG
                     AGNYGMSGRAYDPRFLFSWPSAQSAVMGPQQLAGVLSIVARASAEGKGVEYDEDADKA
                     MREAVEAQIESEQTALANSSRGYDDGIIDPRDTRTVLGMALSAVHNEEVKGAEGFGVF
                     RM"
     gene            1186..3162
                     /locus_tag="VFY44_01140"
     CDS             1186..3162
                     /locus_tag="VFY44_01140"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX5909064.1"
                     /translation="MQRVLVANRGEIARRIFRTCRRLGIGTVAVYSEADAEARFVREA
                     DRAVAIGGPAPGDSYLRGDAVVQAALDAGADAVHPGYGFLAENAAFARSVSDAGLTWI
                     GPAPEAIEAMGSKTEARDRMEKAGVPVLPGRRLDGESGDALAAIADQIGYPLLVKASA
                     GGGGKGMRLVESAGELEGGVEGARREAAGAFGDDTVFLERFAPRSRHVEIQIMGDMEG
                     AVAALGERDCSVQRRHQKVIEESPSPAVDEALRTRMSEAAVAAGQALGYVGAGTVEFL
                     LTEDGEFFFLEVNTRLQVEHPVTELVTGLDLVELQLAVAEGGQLPQAAMRPELRGHAV
                     EARLYAEDAANDFLPVTGTLSRFRIPEGVRVDTGVEDGAEISPYYDPMVAKVIAYGPD
                     RASAVRLLADALTRAELHGPTTNRDFLVGVLRHEEFADGRADTSFLDTHDPAGLAGSL
                     LSEHEQRLAVAAAALALQAARREQATVLASAPSGWRNVPAAPHEVTFADGDRDVVVAY
                     AFDRVNRLVTLQVDGQEIEGAVLHDAAPDHVDLEAGGHRRRFGVHAELSGAVHVNCDA
                     GQLSLVEQPRHPQIEDAAAAGSLASPMPGSVWKVLAAAGDDVDKGQPLLIIEAMKMEH
                     EIVAPVAGPLEELRVAEGDQVDAGTILAVIGEAG"
     gene            3159..>3362
                     /locus_tag="VFY44_01145"
     CDS             3159..>3362
                     /locus_tag="VFY44_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5909065.1"
                     /translation="MSDNVLYEVADAVATITLDDPDTRNALGNETLDELIEAFELARD
                     DDGVRCVVLASSHEKVFSVGGNLD"
BASE COUNT          486 a         1184 c         1262 g          430 t
ORIGIN      
        1 cagctggtgg agtccggcgg cgcggacctg cgcacgcagg cggagggctt catccccggc
       61 ggcgcgacct tcaagaacat cacccgccac tcggcgatgg gcatcccgac ggtgtcgctg
      121 gtgttcggca actccaccgc cggcggcgcg tacgtgcccg gcatgtcgga ctacaacatc
      181 ttcgtgaagg agcgggcgaa ggtcttcctg ggcgggccgc cgctggtgaa gatggccacg
      241 ggcgaggtgt ccgaggacga ggagctcggc ggcgccgaca tgcacgcgcg cgtgagcggg
      301 ctggccgact acctggccaa cgacgagtac gacgcgatcc gcctcggccg cgaggtggta
      361 cggcggctga actggcgcaa gaagggaccg ggtcccaccg agtcgcccga cccgccggtg
      421 cacgaccccg acgagctgct gggcatcgtg ccgcccgacc tcaaggcccc gttcgacccg
      481 cgcgaggtca tcgcccgcgt ggtcgacggc tcacgctacg acgagttcaa ggcgctctac
      541 ggcccctccc tgaccaccgg ctgggcgtcc atccacggct accccgtggg catcatcgcc
      601 aacgtccagg gcgtgctgtt cagccccgag gcgcagaagg cggcgcagtt catccagctg
      661 gccaaccaga aggacgtgcc gctggtgttc atgcacaaca ccaccggcta catggtgggc
      721 aaggactacg agcagggcgg gatcatcaag cacggcgcgc agatgatcaa cgcggtctcc
      781 aactccaagg tcccgcacct cggcgtgatc atggccgcct cctacggcgc cgggaactac
      841 ggcatgtcgg gccgggccta cgacccgcgc ttcctgttct cctggccctc cgcccagtcc
      901 gccgtcatgg gcccgcagca gctggcgggc gtgctgtcga tcgtcgcccg cgcctccgcc
      961 gagggcaagg gcgtcgagta cgacgaggac gccgacaagg ccatgcgcga ggcggtcgag
     1021 gcccagatcg agtccgagca gacggcgctg gccaacagct cgcgcggcta cgacgacggc
     1081 atcatcgacc cgcgcgacac gcgcactgtg ctgggcatgg cgctcagcgc ggtgcacaac
     1141 gaagaggtca agggcgccga gggcttcggc gtcttccgga tgtagatgca acgcgtcctt
     1201 gtcgccaacc ggggcgagat cgcccgccgc atcttccgca cctgccggcg gctgggcatc
     1261 ggcaccgttg ccgtgtactc cgaggccgat gccgaggcgc ggttcgtgcg cgaggccgac
     1321 cgcgcggttg ccatcggcgg ccccgcgccg ggcgactcgt acctgcgcgg tgacgcggtg
     1381 gtgcaggccg cgctggacgc cggcgccgac gcggtgcacc ccgggtacgg cttcctggcc
     1441 gagaacgcgg cgttcgcgcg ctcggtctcc gacgcgggcc tgacctggat cggcccggcg
     1501 ccggaggcga tcgaggcgat gggctccaag accgaggccc gcgaccggat ggagaaggcg
     1561 ggtgtgcccg tgctgccggg ccgcaggctc gacggcgagt cgggtgacgc gctggcggcg
     1621 atcgccgatc agatcggcta cccgctgctg gtcaaggcgt cggccggcgg cggcggcaag
     1681 ggcatgcgcc tggtcgagtc cgcgggcgag ctggagggcg gcgtcgaggg cgcgcggcgc
     1741 gaggccgccg gggcgttcgg cgacgacacc gtgttcctgg agcgcttcgc gccccggtcg
     1801 cgtcacgtgg agatccagat catgggcgac atggagggtg cggtggcggc cctgggcgag
     1861 cgcgactgct cggtacagcg gcgccaccag aaggtcatcg aggaatcgcc ctcgcccgcc
     1921 gtcgacgagg ccctgcgcac ccggatgagc gaggccgcgg tggccgccgg ccaggcgctg
     1981 ggctacgtgg gcgcgggcac ggtcgagttc ctgctgaccg aggacggcga gttcttcttc
     2041 ctggaggtga acacccggct gcaggtcgag cacccggtga ccgagctggt cacgggcctg
     2101 gacctggtcg agctccagct ggcggtggcc gagggtggcc agctcccgca ggcggccatg
     2161 cgccccgagc tgcgcggcca cgcggtcgag gcgcggctgt acgccgagga cgcggccaac
     2221 gacttcctgc cggtgacggg cacgctcagc cggttccgga tccccgaggg cgtgcgcgtg
     2281 gacaccggcg tggaggacgg cgccgagatc agcccctact acgacccgat ggtggccaag
     2341 gtcatcgcgt acggccccga ccgggcgagc gccgtgcgcc tgctggccga cgccctcacc
     2401 cgcgccgaac tgcacggccc caccaccaac cgcgacttcc tggtcggcgt gctgcgccac
     2461 gaggagttcg cggacgggcg cgccgacacg tccttcctcg acacccacga ccccgccggg
     2521 ctggccgggt cgctgctcag cgagcacgag cagcgcctgg ccgtggccgc cgccgcgctg
     2581 gcgttgcagg cggcccgccg cgagcaggcg accgtgctgg ccagcgcacc cagcggctgg
     2641 cgcaacgtgc ccgcggcgcc gcacgaggtc accttcgccg acggcgaccg cgacgtggtc
     2701 gtggcctacg cgttcgaccg cgtgaaccgc ctggtcaccc tgcaggtgga cggccaggag
     2761 atcgagggcg cggtcctgca cgacgcggcc cccgaccacg tggacctgga ggccggcggc
     2821 caccggcgcc ggttcggggt gcacgccgag ctctcgggcg ccgtgcacgt gaactgcgac
     2881 gccggccagc tgtcgctggt cgagcagccg cgccacccgc agatcgagga cgcggccgcg
     2941 gcgggctcgc tggcctcgcc catgcccggg tcggtgtgga aggtgctggc cgccgcgggc
     3001 gacgacgtgg acaagggcca gccgctgctg atcatcgagg cgatgaagat ggagcacgag
     3061 atcgtggccc cggtggccgg cccgctggag gagctgcgcg tggccgaggg cgaccaggtg
     3121 gacgcgggca ccatcctcgc ggtgatcggg gaggccgggt gagcgacaac gtgctgtacg
     3181 aggtggccga cgcggtggcc acgatcaccc tcgacgatcc cgacacccgc aacgcgctgg
     3241 gcaacgagac gctggacgag ctgatcgagg ccttcgagct ggcgcgcgac gacgacgggg
     3301 tgcgctgcgt ggtgctggcc tcctcgcacg agaaggtgtt ctcggtcggc ggcaacctgg
     3361 ac
//
LOCUS       DASUPW010000051         5110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1535116, whole genome shotgun sequence.
ACCESSION   DASUPW010000051 DASUPW010000000
VERSION     DASUPW010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535116"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VFY44_01150"
     CDS             <1..448
                     /locus_tag="VFY44_01150"
                     /inference="COORDINATES: protein motif:HMM:NF016818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HEX5909066.1"
                     /translation="AAEPGHELGYAGSRLHWIPGAPEGWALLVGDETGLPALSAIAEG
                     LPAGMRAIALVEVEDAGERQPIDSAADLDLRWLYRDGAPAGETTLLADAVRELELPDG
                     PGRAWGGGEALAMRDVRRHLKDERGLPGQDTQILGYWKHRATESWE"
     gene            465..1217
                     /locus_tag="VFY44_01155"
     CDS             465..1217
                     /locus_tag="VFY44_01155"
                     /inference="COORDINATES: protein motif:HMM:NF042851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HEX5909067.1"
                     /translation="MPRTVAATRYVAPLRQGGSLPALVEADDDGLYVVKLRGAGQGPR
                     ALVSELVAGGIGAALGLPVPELVLVDLDPHLADAEPDPEIQELLQASAGLNAGLDFLP
                     GSLPYLPGGSLAVEPETAAAVVWLDALTENIDRTPRNTNLLVWHGRLWLIDHGAALYR
                     QHTGLDPAQAGGAFEAIADHVLLPCAGSISDADASLAPVVDRDLLTGLLAGVPDDWLL
                     GTPPADYVDYLAARLEPPRAWVAQAEEARARG"
     gene            1210..1593
                     /locus_tag="VFY44_01160"
     CDS             1210..1593
                     /locus_tag="VFY44_01160"
                     /inference="COORDINATES: protein motif:HMM:NF022681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HEX5909068.1"
                     /translation="MAEGFQYALLRAVPSIERGEALNVGVVVHCRRAGFLGVRTAVDP
                     ERLRLLDPRVDVAAVDAHLGVIERVAAGQEPDSPIAAMDRSDRFGWIVAPSSTSVQPS
                     PVHTGLTDDPAATLDKLFTELVLVA"
     gene            1590..2900
                     /locus_tag="VFY44_01165"
     CDS             1590..2900
                     /locus_tag="VFY44_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5909069.1"
                     /translation="MSATELPPGPRRPALAQTLQWVFDPERFMRKSRERYGSTFTARL
                     GPSADVIFLCEPEDIKTVFRGTPEETNMGDINGLFRRVLGSNSLLVIDGDEHLRQRRL
                     LLPPFHGERIAEHYGAMLEAAEEDVAGWEEGQTLTLQPHMQAMTLNVILAAVFGLGPG
                     ERRDRFRVLLARLLELNTTLATTLPQLRIELGGRSPWGKLMRCTAEVDELLYDEITRR
                     RAQRDRTSGDVLSLLLDAQDADDGQAMTDRELRDQLLTMLVAGHETTATALAWAFERL
                     LRNPAVLVRVTGELAQGSTTYLDATIKEVLRLRPVVPITARKITVPFEVGGRTYEPGT
                     VLMPCIYLLHRHPDIYERPEEFLPERFIEHSPPPYSWIPFGGGVRRCLGSGFALAEMR
                     AVMQTVLTRVQLAPEREASERVVRRAFTISPREGARVVVQQRLG"
     gene            3041..4774
                     /locus_tag="VFY44_01170"
     CDS             3041..4774
                     /locus_tag="VFY44_01170"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5909070.1"
                     /translation="MDDRTRVNEAGHGRPHPDGRALTPGLAAKAQAYLFLTAGALGAL
                     GVMLPHPPRFDEVGMLTVQLASVVTAFGLLALGDRTPRWFTRIGPYMAVLATTGVLLF
                     THSDASPYLLFYLWVAFYAFYFLVTREAVFLAAFATLNFALVLVCFRTGLVGTPEVGS
                     NEDVSAFVLLTGTVAVAGAFIVLLRSRVTRLIGQLSDAASIDPMTGLFNRRGFQDAVE
                     RELSRAERSNQPVSVLQADCDLFKSYNIRLGNEAGDSALRRIGRMLEDDRRRVDVAAR
                     IGGEEFGVILPDTDQHEAFLVAERLRMRIAEMFADQPIPLTVSFGVVTYPVHARDSEG
                     LQVAAENALYAAKVLGRDRSVLHSEEVAEILSAGGDGAHQRDEAQLATVLNLAEALDM
                     RDEGTARHSQTVGRYCEMMARELGFSREHADRVRVAGVLHDIGKIGVADSILQKPGPL
                     TDAEYEAMKKHPEIGARILGGSGLADIRQWVLAHHERPDGRGYPSGKSGDGVPLEARI
                     LSVGDAYEAMTSDRVYRKAIGHEAARDELCKWAGKQFDADVVEAFLRALDTDPSFQLS
                     APARSGGSGAR"
     gene            complement(4734..>5110)
                     /locus_tag="VFY44_01175"
     CDS             complement(4734..>5110)
                     /locus_tag="VFY44_01175"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5909071.1"
                     /translation="LKAAEDEPRQVADLVNISSVAGRSANKGSAVYNATKHAVGAFSE
                     ALRQEVTDRHVRVSLMEPGAVHTELASQSRDGVREGVEKMYNSMESLEAVDIADAISY
                     IVTRPRRVAINELLIRPTEQVR"
BASE COUNT          669 a         1871 c         1864 g          706 t
ORIGIN      
        1 cgccgccgag cccggccacg agctgggcta cgcgggctcg cgcctgcact ggatccccgg
       61 ggcgccggag ggctgggcgc tgctggtggg cgacgagacc ggcctgcccg cgctctcggc
      121 gatcgccgag gggcttcccg cgggcatgcg cgccatcgct ctggtcgagg tcgaggacgc
      181 cggcgagcgc cagccgatcg acagcgcggc cgacctggac ctgcgctggc tgtaccgcga
      241 cggcgccccg gcgggcgaga ccacgctgct ggccgacgcg gtgcgcgagc tcgagctgcc
      301 ggacgggccg ggccgggcgt ggggcggggg cgaggcgctg gccatgcgcg acgtgcgccg
      361 gcatctcaag gacgagcgcg gcttgcccgg ccaggacacc cagatcctgg gctattggaa
      421 gcaccgcgcg accgagtcgt gggagtgaga gtcttcgcgg cctgatgccg cgcaccgtcg
      481 ccgccacccg ctacgtggcc cccctgcgcc aggggggctc gctgcccgcg ctggtggagg
      541 ccgacgacga cggcctgtac gtggtcaagc tccggggcgc ggggcaggga ccgcgggcgc
      601 tggtgtccga gctcgtggcc ggcgggatcg gcgccgcgct gggcctgccg gtgcccgagc
      661 tggtgctggt cgacctggac ccgcacctgg ccgacgccga gcccgacccc gagatccagg
      721 agctgctcca ggccagcgcc ggcctgaacg cgggcctgga cttcctgccc gggtcgctgc
      781 cctacctgcc gggcgggtcg ctggcggtgg agcccgagac cgccgccgcc gtggtctggc
      841 tggacgcgct gaccgagaac atcgaccgca cgccgcgcaa caccaacctg ctggtctggc
      901 acgggcggct gtggctgatc gaccacggag cggcgctcta ccgccagcac acggggctgg
      961 accccgccca ggccggcggc gccttcgagg ccatcgccga ccacgtgctg ctgccgtgcg
     1021 cgggctcgat ctccgacgcg gatgccagtc tggcgcccgt ggtggaccgc gacctgctca
     1081 ccggcctgct ggccggcgtc cccgacgact ggctgctggg caccccgccc gcggactacg
     1141 tcgactacct ggcggcgcgg ctcgagccgc cgcgcgcgtg ggtggcgcag gccgaggagg
     1201 cccgggcccg tggctgaggg cttccagtac gcgctgctgc gcgcggtgcc gtcgatcgag
     1261 cggggcgagg ccctgaacgt gggcgtggtc gtgcactgcc gccgcgccgg gttcctgggc
     1321 gtgcgcacag ccgtcgaccc ggagcgcctg cggctgctgg acccgcgcgt cgacgtggcc
     1381 gcggtcgacg cccacctggg cgtgatcgag cgcgtcgccg cgggccagga gcccgacagc
     1441 ccgatcgcgg ccatggaccg ctccgaccgc ttcggctgga tcgtggcgcc gtccagcacg
     1501 tcggtccagc cctcgccggt gcacaccggc ctgaccgacg acccggcggc cacgctggac
     1561 aagctgttca ccgagctggt gctggtcgct tgagcgccac cgagctgccg cccggcccgc
     1621 gccgccccgc gctggcccag acgctccagt gggtcttcga ccccgagcgg ttcatgcgca
     1681 agtcccgcga gcggtacggc agcacgttca ccgcccggct gggccccagc gccgacgtga
     1741 tcttcctgtg cgagcccgag gacatcaaga cggtgttccg gggcacgccc gaggagacga
     1801 acatgggcga catcaacggc ctgttccggc gcgtccttgg ctcgaactcg ctgctggtga
     1861 tcgacggcga cgagcacctg cgccagcgcc ggctgctgct gcccccgttc cacggcgagc
     1921 gcatcgccga gcactacggc gccatgctcg aggccgccga ggaggacgtg gccggctggg
     1981 aggagggcca gacgctgacg ctgcagccgc acatgcaggc catgaccttg aacgtgatcc
     2041 tggcggcagt gttcggcctg gggccgggcg agcgccgcga ccgcttccgc gtcctgctcg
     2101 cgcgcctgct ggagctgaac accaccctgg ccacgacgct gccgcagctg cggatcgagc
     2161 tgggcgggcg ctccccgtgg ggcaagctca tgcgctgcac ggccgaggtc gacgagctgc
     2221 tctacgacga gatcacccgc cgccgggcgc agcgcgaccg cacctcgggg gacgtcctgt
     2281 cgctcctgct tgacgcccag gacgccgacg acggccaggc catgaccgac cgcgagctgc
     2341 gcgaccagct gctgacgatg ctggtggcgg gccacgagac cacggccacg gccctggcct
     2401 gggccttcga gcggctgctg cgcaaccccg cggtgctggt gcgcgtgacc ggtgagctgg
     2461 cccagggcag caccacctac ctggacgcca ccatcaagga ggtgctgcga ctgcggccgg
     2521 tcgtgcccat caccgcgcgc aagatcacgg tgccgttcga ggtcgggggg cgcacgtacg
     2581 agccgggcac ggtgctgatg ccgtgcatct acctgctgca ccgccacccc gacatctacg
     2641 agcgcccgga ggagttcctg cccgagcgct tcatcgagca cagcccgccg ccctacagct
     2701 ggatcccgtt cggcggcggg gtcaggcgct gcctggggtc gggcttcgct ctggccgaga
     2761 tgcgggccgt catgcagacg gtcctgacgc gggtccagct ggcacccgag cgcgaggcgt
     2821 ccgagcgcgt ggtcaggcgc gcgttcacga tctcaccgcg cgagggcgcg cgggtggtcg
     2881 tgcagcagcg gctgggctag gcgcgccgcg ccaggcggcg cagcgagcgg cgggcaccgg
     2941 cgatggccga ggccagcacc aggtccagcg aaggtccgga gaggggggtc atgcaatcca
     3001 cttcgccgat ccgggccccg ggcttgagga atccggacgt atggacgata ggacgcgtgt
     3061 gaacgaagct ggacacggac gtccacatcc cgacggccgg gcgctgacgc ctgggctggc
     3121 ggcgaaggcg caggcctacc tgttcctgac cgcgggcgcc ctcggcgcgc tcggcgtgat
     3181 gctgccgcac cccccgcgct tcgacgaggt gggcatgctg acggtccagc tggcctcggt
     3241 ggtcacggcc ttcgggctcc tggccctggg cgaccgcaca ccgcgctggt tcacccgcat
     3301 agggccctac atggcggtgc tggccaccac cggcgtcctg ctgttcaccc actccgacgc
     3361 cagcccgtac ctgctcttct atctctgggt cgcgttctac gccttctact tcctggtcac
     3421 ccgggaggcg gtcttcctgg cggccttcgc caccctgaac ttcgcgctcg tgctggtctg
     3481 cttccgcacc gggctggtcg gcacccccga ggtgggctcc aacgaggacg tctccgcctt
     3541 cgtgctcctg accggcaccg tcgccgtggc gggcgccttc atcgtgctgc tgcgctcgcg
     3601 cgtgacgcgc ctgatcggcc agctcagcga cgcggccagc atcgacccga tgacgggcct
     3661 gttcaaccgc cgcgggttcc aggacgcggt cgagcgcgag ctctcgcgcg ccgagcgcag
     3721 caaccagccg gtcagcgtgc ttcaggccga ctgcgatctg ttcaagagct acaacatccg
     3781 tctcggtaat gaggcgggtg actcggcgct gcgccggatc gggcggatgc tcgaggacga
     3841 ccgccggcgc gtggacgtgg ccgcgcggat cggcggcgag gagttcggcg tgatcctgcc
     3901 cgacaccgac cagcacgagg ccttcctggt ggccgagcgg ctgcgcatgc gcatcgccga
     3961 gatgttcgcc gaccagccga tcccgctgac cgtgagcttc ggcgtggtca cctaccccgt
     4021 gcacgcccgc gacagcgagg ggttgcaggt cgcggccgag aacgccctct acgcggccaa
     4081 ggtcctgggc cgtgaccgca gcgtcctgca cagcgaggag gtggccgaga tcctgtccgc
     4141 cggcggcgac ggcgcgcacc agcgcgacga ggcgcagctg gccaccgtgc tcaacctggc
     4201 cgaggcgctg gacatgcgtg acgagggcac cgcccgccac tcccagaccg tggggcgcta
     4261 ctgcgagatg atggcccgcg agcttggctt ctcccgcgag cacgccgacc gcgtccgcgt
     4321 ggccggggtg ctgcacgaca tcggcaagat cggcgtggcc gactcgatcc tgcagaagcc
     4381 gggcccgctg accgacgccg agtacgaggc catgaagaag caccccgaga tcggcgcccg
     4441 gatcctgggc gggtcggggc tggccgacat ccgccagtgg gtgctggccc accacgagcg
     4501 ccccgacggc cgcggctacc ccagcggcaa gtcaggcgac ggggtgccgc tggaggcgcg
     4561 catcctgtcc gtgggcgacg cctacgaggc catgaccagc gaccgcgtgt accgcaaggc
     4621 catcggccac gaggcggccc gcgacgagct gtgcaagtgg gcgggcaagc agttcgacgc
     4681 cgacgtggtc gaggcgttcc tgcgggccct ggacacggac ccgtcgttcc agctcagcgc
     4741 acctgctcgg tcgggcggat caggagctcg ttgatcgcca cccgccgcgg gcgggtcacg
     4801 atgtagctga tggcgtcggc gatgtcgacg gcctccaggc tctccatgct gttgtacatc
     4861 ttctcgacgc cctcgcggac gccgtcgcgg ctctggctgg ccagctcggt gtgcaccgcg
     4921 ccgggctcca tcagcgacac gcgcacgtgg cggtcggtga cctcctggcg cagcgcctcg
     4981 ctgaacgcgc ccaccgcatg cttggtggcg ttgtagaccg cgctgccctt gttggccgag
     5041 cgtcccgcca ccgacgagat gttcaccagg tcggccacct ggcgcggctc gtcctcggcc
     5101 gccttcagca
//
LOCUS       DASUPW010000052         8326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_704189, whole genome shotgun sequence.
ACCESSION   DASUPW010000052 DASUPW010000000
VERSION     DASUPW010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8326
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704189"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..329)
                     /locus_tag="VFY44_01180"
     CDS             complement(87..329)
                     /locus_tag="VFY44_01180"
                     /inference="COORDINATES: protein motif:HMM:NF039758.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6411 family protein"
                     /protein_id="HEX5909072.1"
                     /translation="MVIGAIVAVCVVLLILAFVFPRLSRHPQRGVDSTLSAGQKAGGI
                     LPGFLGRWAQKPFGKSRKATNKSASTGRRARGKMPL"
     gene            491..1798
                     /locus_tag="VFY44_01185"
     CDS             491..1798
                     /locus_tag="VFY44_01185"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX5909073.1"
                     /translation="MIERQQIAEALSRLDPRDREILDYSLRRRVPDEDLAALFEAEPA
                     EVARRRAAAIETLSGDLGAQRGADLGHILKELLEADTWEAAAAAAPAAERSEAEPSEP
                     TEAEPAAAAPEAEEPAPDHPPLGASARKPSEDPPEAARPGGEPVLGMLSGQEQPAAAP
                     AEPDPPSRNRGRRRLLVGVAAAAAVLVPAGMVAALATSEDSDNGPSGSASSATREFAP
                     AREGGTGSAEPFPSDPQSAEQYPIARVNRRTALYREPGGKVRLRIAPKTEWDSPRILS
                     VVERRGRWLAVLVPELKNGQQAWVRDDKVANISSVTWALHADLSKRTLEVRHNGKAVR
                     TMKIGVGRTDHPTPVGRYAVTDKLRVSDPGSPYGCCVLALTGHQTRLPQGWPGGDRLA
                     VHATRDLSGLGRRVSLGCMRTDPRDTKWMMKRVPLGTPVFVDS"
     gene            1837..2307
                     /locus_tag="VFY44_01190"
     CDS             1837..2307
                     /locus_tag="VFY44_01190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5909074.1"
                     /translation="MSLPPFQVLLDQHGPAVHRFLLASVGASDADDCYQEACIAALRA
                     YPDLEHGQNLKGWLMTVAHRKAVDAHRARARRPVPVERVPEQASRDGAPSDAEDPLWA
                     QVRELPDKQRAAVFLRSVGDLSYAEVATALGCSQDAARRSVHEGLKRLRQEVPS"
     gene            2304..2852
                     /locus_tag="VFY44_01195"
     CDS             2304..2852
                     /locus_tag="VFY44_01195"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX5909075.1"
                     /translation="MRELNLLVAERAQREGLLDVAYAMVDSPFGPLTAAATDRGLVML
                     AYPEHEPGAVLERLAGELSPRVLEAPARLDGVRRELAEYFEGRRQEFETPVDFSLSRG
                     FYRKVLEATARIGYGQTTTYAGVAADAGNPKAVRAAGSGLGSNPIPVVVPCHRVLRTG
                     GALGGYTGGLERKEFLLSLERR"
     gene            complement(2798..3613)
                     /locus_tag="VFY44_01200"
     CDS             complement(2798..3613)
                     /locus_tag="VFY44_01200"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HEX5909076.1"
                     /translation="MTIRRILALGGGGFTSSSEDWPLDELTPDLADAERPAICLLPTA
                     SGDPEDQIARFYDAFGPLDCELSHISLFRLGSQAVDLRRHLLSRDVIYVGGGSMLNLL
                     ALWRVHDVDSILSEAYQRGIVLCGVSAGAMCWFDAGVTRTHGAPGPATGLGLLPGTLS
                     VHDSTDPQRRAEFRDQVAAGMAGGWAAEDGVGLLFEDGLLTEAVTARRGARAYRVWRG
                     RDGRVAEEQIEPRLLDADAEGRAPDPVSIVEYREAAQRRSRDSRNSLRSRPPV"
     gene            complement(3610..4377)
                     /locus_tag="VFY44_01205"
     CDS             complement(3610..4377)
                     /locus_tag="VFY44_01205"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HEX5909077.1"
                     /translation="MSAPRRQVVAFGGGGFSMEAGNPLLDDYVLSLAGADRPRVCFLP
                     SASGDADHYIVRFYRAFAAGRCEPSHISLFRRDHGPADLHEHLLSQDIVYVGGGCVLS
                     LLGVWGAHGIDRILREAWEAGVIMCGVSAGSLCWFSEGVTAFHGAPRPYTGLGLLPWS
                     NTVHYDAERCREGEYRRLLREGMRPGYAAEDGAALHFVGDRLHRVVASRPGARAYRMR
                     KLPNGRVARKAVRTTYLGGGAAAPEPPLRVAPQPLAA"
     gene            complement(4463..5086)
                     /locus_tag="VFY44_01210"
     CDS             complement(4463..5086)
                     /locus_tag="VFY44_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909078.1"
                     /translation="MRKVLLAAAAILVVSLVATQVILPRVISGEVEKRLTQDGGTAEV
                     KITAVPALTLAWSRGRSIEISGDDLAYDLDERKERPFERLDGFGDVRIDLRDLDAGSV
                     KLTSFTLTREGRDRPYELSMRGTSTPRDLAAELGTATGGTLGGLLGGLASGVLPDAGL
                     GSVPLQMDATVGSEDGRPRVDQARATVAGVPAGRLTEVVLRAVLERL"
     gene            complement(5120..5209)
                     /locus_tag="VFY44_01215"
     tRNA            complement(5120..5209)
                     /locus_tag="VFY44_01215"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5173..5175),aa:Ser,seq:gct)
     gene            complement(5233..5478)
                     /locus_tag="VFY44_01220"
     CDS             complement(5233..5478)
                     /locus_tag="VFY44_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909079.1"
                     /translation="MALMLIDTREPEPEPEPRRRRRDIDWRLWFWSFESVGLFVAASS
                     LAGFAAVGLAFGGIFAAARAFDAFSGGWGTGLRDHRQ"
     gene            complement(5495..6547)
                     /locus_tag="VFY44_01225"
     CDS             complement(5495..6547)
                     /locus_tag="VFY44_01225"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5909080.1"
                     /translation="MLTPTQHAAREERKTIAYSAAAIFVGAVAIAALETALPGGPENS
                     IVPGIAAVVMGAIAVTVGPKMPRKWLAVFAPIGVACIAYALAETRGGPSDGAVLYMWP
                     VLWMSYFYGRAGAVFIVAFVALAQGVALLTMPAELVSADRWIDVVASVTVVAAVVRYL
                     ASRNARLVRDLEEQARVDPLTGLLNRRGLEERMSIEVNRALRQKTPMAMAMVDIDHFK
                     DVNDAHGHEMGDRVLAWLGGVLARQSRGVDVAARTGGDEFAVLLPDTSAEDGRRYADR
                     VVRAIAGPDAAAERAQYGLPEGLELTVSVGVSGTGAADASMLMDTADKALYAAKGEGR
                     NRVVAADLDRLHPARV"
     gene            6746..7918
                     /locus_tag="VFY44_01230"
     CDS             6746..7918
                     /locus_tag="VFY44_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEX5909081.1"
                     /translation="MRELNRSRVIEALRELGVASRADVARHTGLSRTTISGLVAEMRD
                     EGLVVDRPGPDGDAPASGAGRPPTPIGLSARAGAALGIDFGKRHLAVVVADLSHTLLA
                     EDQREMEEGYDSAAGIDAAIELVEDVLARAGIPVRQLLGVGVGLPGPVHGPSGQVGSS
                     AILPGWEGLQVAREMEGRLGVPVRVDNDANLGALAELFWGAGEGAADLVYLKVATGIG
                     AGLIFQGRLFHGAGGTAGEIGHLTIDEHGPMCRCGNRGCLETFAAGDALVELLRSSLG
                     HELTAEEIVDQAIAGDVACRRAIADAGRHLGHAVGAICNLINPSRVVVGGSIGQAGDL
                     LLGEMREAVRRRAVLSAAQDVEIVAGVLGERAEVLGAVALVLGDAEAAPVPEAVTT"
     gene            8006..>8326
                     /locus_tag="VFY44_01235"
     CDS             8006..>8326
                     /locus_tag="VFY44_01235"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX5909082.1"
                     /translation="MVAGCGTSDEDDSGGGDSGGGSKSGKIALLLPESKTARYESQDR
                     PNFEKKVKELCADCEIIYSNADQDAAKQQQQADAALTKGAKVMVLDPVDAASAGGIVA
                     RAKQK"
BASE COUNT         1156 a         3063 c         3103 g         1004 t
ORIGIN      
        1 ccggtcgacc cggccaccgc cgaggtcgag ctcgacgtct tcgaaggtgg cgcggccgcc
       61 gacgacgacc gcgacgacga caccgtctag agcggcatct tcccgcgcgc gcggcgcccg
      121 gtcgacgcgc tcttgttggt ggccttgcgc gacttgccga acggcttctg cgcccagcgg
      181 cccaggaaac cgggcaggat cccgccggcc ttctggcccg cgctcagggt gctgtccacg
      241 ccgcgctggg ggtgccgcga caggcgcggg aacacgaacg ccaggatcag caggacgacg
      301 catacggcga cgattgctcc tatgaccatg cttctctcct ccttcggggt ccccccgggc
      361 taccccggca ggggtagagc ccaaactaaa gcccggggcc ccggggcccg atctagccag
      421 caggtgcgat ggctaggctg gaccggtaag acggcccgcc gcttccgccg tcgccgcaga
      481 ccgccccacc atgatcgaac gccaacagat cgccgaggcg ctcagccgcc tcgatccccg
      541 cgaccgggag atcctcgact actcgttgcg ccgccgcgtt ccggacgagg acctggccgc
      601 gctgttcgag gccgagccgg ccgaggtggc ccggcgccgc gcggccgcga tcgagaccct
      661 gtcgggcgac ctgggcgcgc agcggggcgc cgatctgggc cacatcctca aggagctgct
      721 cgaggccgac acgtgggagg cggccgccgc cgcggcgccc gcggccgagc ggtccgaggc
      781 cgagccgtcc gagccgacgg aagccgagcc cgccgctgcg gcaccggagg ccgaggagcc
      841 cgctcccgac cacccgccgc tgggcgccag cgccaggaag ccctccgagg acccgcccga
      901 ggccgcccgc cccggcggcg agccggtcct gggcatgctc tcgggccagg agcagccggc
      961 cgccgcgccg gccgagcccg accccccgag ccgcaaccgc ggccggcgcc gcctgctggt
     1021 gggcgtggcc gccgcagcgg ccgtgctggt gcccgcgggc atggtggccg ccctggccac
     1081 cagcgaggac tccgacaacg gcccgtcggg ttccgcgtcg tcggccacgc gcgagttcgc
     1141 cccggcgcgc gagggcggca ccggctcggc cgagccgttc ccgtccgacc cgcagagcgc
     1201 cgagcagtac ccgatcgcgc gcgtgaacag gcgtaccgcg ctctaccgcg agcccggggg
     1261 caaggtgcgg ctgcggatcg cgccgaagac ggagtgggac tcgccccgca tcctcagcgt
     1321 cgtcgagcgc cgcggccgct ggctggccgt gctggtgccc gagctcaaga acggccagca
     1381 ggcctgggtc cgcgacgaca aggtggccaa catcagcagc gtcacgtggg cgctgcatgc
     1441 cgacctgtcc aagcgcaccc tggaggtgcg ccacaacggc aaggccgtgc gcacgatgaa
     1501 gatcggcgtc gggcgcaccg accacccgac gcccgtgggc cgctacgcgg tcaccgacaa
     1561 gctgcgcgtc agcgatcccg gctcgccgta cggctgctgc gtgctggcgc tgaccggaca
     1621 ccagacgcgc ctcccgcagg gttggcccgg cggggaccgc ctggccgtgc acgccacccg
     1681 cgacctcagc ggcctgggcc ggcgcgtctc gctgggctgc atgcgcaccg acccgcgcga
     1741 caccaagtgg atgatgaagc gcgtcccgct gggcaccccc gtgttcgtcg actcctagac
     1801 ctcacaccgg gcggtctgcc agcgttgaag gggcacatgt ccctgccgcc gttccaggtg
     1861 ttgctggacc agcacggccc cgccgtgcac cggttcctgc tggcctcggt cggagcgagc
     1921 gacgcggacg actgctacca ggaggcctgc atcgccgccc tacgcgcgta tccggatctc
     1981 gagcacggcc agaacctcaa gggctggctg atgaccgtgg cccaccgcaa ggcggtggac
     2041 gcccaccggg cccgcgcgcg ccgcccggtg ccggtggagc gcgtccccga gcaggcctcc
     2101 cgcgacgggg cgccgagcga cgccgaggac ccgctctggg cgcaggtgcg cgagctgccc
     2161 gacaagcagc gcgccgcggt gttcctgcgc agcgtcggcg acctgtccta cgccgaggtg
     2221 gcgacggcgc tgggctgctc gcaggacgcg gcccggcgca gcgtgcacga gggcctcaag
     2281 cggctgcgcc aggaggtgcc ctcgtgaggg agctgaacct gctggtggcc gagcgcgccc
     2341 agcgcgaggg gctcctggac gtggcctacg cgatggtgga ctcgccgttc gggcccctga
     2401 ccgcggccgc caccgaccgc gggctggtca tgctcgccta ccccgagcac gagccgggcg
     2461 cggtgctgga gcgcctggcc ggcgagctgt cgccgcgcgt gctggaggcg cccgcgcggc
     2521 tggacggggt ccggcgcgag ctggccgagt acttcgaggg ccggcggcag gagttcgaga
     2581 ccccggtgga cttctcgctc tcgcgcggct tctaccggaa ggtgctggag gccaccgccc
     2641 ggatcggcta cgggcagacc accacgtacg ccggcgtcgc ggccgacgcc ggcaacccca
     2701 aggcggtgcg cgcggcgggc agcgggctgg gctcgaaccc gatcccggtg gtggtgccct
     2761 gccaccgcgt gctgcgcacc ggcggcgcgc ttggcggcta caccggcggc ctggagcgca
     2821 aggagttcct gctgtccctc gagcgccgct gagcggcctc gcggtactcc acgatcgaca
     2881 ccggatcggg cgcccgcccc tcggcgtcgg cgtccagcag ccgcggctcg atctgctcct
     2941 cggccacccg accgtcccgg ccgcgccaga cccggtaggc gcgcgcgccg cggcgggccg
     3001 tcaccgcctc ggtcagcagc ccgtcctcga acagcaggcc cacgccgtcc tcggccgccc
     3061 agccgccggc catgcccgcc gccacctggt cgcggaactc ggcccggcgc tggggatcgg
     3121 tggagtcgtg aaccgaaagc gtgccgggca ggaggcccag ccccgtggcc gggcccggcg
     3181 cgccgtgcgt gcgggtgacg ccggcgtcga accagcacat ggcgccggcg ctgacgccgc
     3241 acaggacgat gccgcgctgg taggcctcgc tgaggatcga gtccacgtcg tgcacgcgcc
     3301 acagcgccag caggttgagc atgctgccgc cgcccacgta gatcacgtcg cgcgagagca
     3361 ggtggcggcg caggtcaacg gcctggctgc ccagccggaa cagcgagatg tgcgacagct
     3421 cgcagtccag cgggccgaac gcgtcgtaga agcgcgcgat ctgatcctcg ggatcaccgc
     3481 ttgccgtggg cagcaggcag atggcgggcc gctcggcgtc ggccaggtcc ggcgtcagct
     3541 cgtccagcgg ccagtcctcc gaggagctgg tgaagccgcc gccgcccagg gccaggatgc
     3601 gccggatggt catgcggcca gcggctgcgg cgccacgcga agcgggggct cgggtgccgc
     3661 ggcgccgccg cccaggtagg tcgtgcgcac cgccttgcgg gccacccggc cgttcgggag
     3721 cttgcgcatc cggtacgcgc gggcgccggg ccgcgaggcc accacccggt gcagccggtc
     3781 gccgacgaag tgcagcgccg cgccgtcctc ggccgcgtac cccgggcgca tgccctcgcg
     3841 cagcaggcgc cggtactcgc cctcgcggca gcgctcggcg tcgtagtgca cggtgttcga
     3901 ccacggcagc aggcccaggc ccgtgtacgg gcgcggcgcc ccgtggaacg ccgtcacgcc
     3961 ctcgctgaac cagcacagcg agccggcgct gaccccgcac atgatcaccc ccgcctccca
     4021 cgcctcgcgc aggatccggt cgatgccgtg ggcgccccag acgccgagca ggctcagcac
     4081 gcagccgccg cccacgtaga cgatgtcctg cgacagcagg tgctcgtgca ggtcggccgg
     4141 gccgtggtcg cgccggaaca gcgagatgtg cgacggctcg cagcgcccag cggcgaaggc
     4201 gcggtagaag cgcacgatgt agtggtccgc gtcgccgctg gcggagggca ggaagcacac
     4261 ccgcgggcgg tccgcccccg cgagcgagag cacgtaatcg tccaggagag ggttgcctgc
     4321 ctccatgctg aacccgcctc ccccgaacgc gaccacctgt cgccgaggcg ccgacacccg
     4381 cgggacggta ggggccgccg gaaggggggt caaagaattt cagcccctgg tccgagcggg
     4441 gttggaccgg gtgtcctagg acctacagcc gttccagcac ggcccgcagc accacctcgg
     4501 tcagccgtcc ggccggcacg cccgccacgg tcgcccgcgc ctggtcgacg cgcgggcggc
     4561 cgtcctcgct ccccacggtg gcgtccatct gcagcggcac cgagcccagg ccggcgtcgg
     4621 gcagcacgcc ggaggccagg ccgccgagga ggccgccgag cgtgccgccg gtcgcggtcc
     4681 ccagctccgc ggccaggtcg cgcggcgtgc tcgtgccgcg catcgagagc tcgtagggcc
     4741 ggtcgcgccc ctcccgggtg agcgtgaacg aggtcagctt caccgagccc gcgtccaggt
     4801 cgcggaggtc gatgcgcacg tcgccgaagc cgtccaggcg ctcgaacggg cgctccttgc
     4861 gctcgtccag gtcgtaggcc aggtcgtcgc cgctgatctc gatcgaccgg ccgcggctcc
     4921 acgccagcgt cagcgcgggc acggcggtga tcttcacctc ggccgtgccg ccgtcctggg
     4981 tcaggcgctt ctcgacctcg ccgctgatca cccggggcag gatcacctgg gtggcgacga
     5041 gtgagacgac caggatcgcg gcggcggcca ggagcacctt gcgcatacaa gcgcagtgtg
     5101 gcgggttcgg gcgatgcgac ggacagggag ggattcgaac cctcgatacg gttctaagcc
     5161 gcatactccc ttagcagggg agcgccttca gccactcggc cacctgtccc gggacggcga
     5221 ttgtatgcgg cgctactgcc ggtggtcgcg cagcccggtg ccccacccgc cggagaacgc
     5281 gtcgaacgcc ctggcggcgg cgaagatgcc gccgaaggcc aggcccacgg cggcgaagcc
     5341 ggccagcgag gacgcggcca cgaacaggcc gacgctctcg aagctccaga accacagccg
     5401 ccagtcgatg tcgcggcggc gccgccgggg ctccggctcc ggctcgggct cgcgggtgtc
     5461 gatcagcatc agggccaccc tgatctgagc ctagctacac gcgtgcgggg tgcaggcgat
     5521 ccaggtccgc ggcgacgacg cggttgcgcc cctcgccctt ggccgcgtac agggccttgt
     5581 cggccgtgtc catgagcatg ctcgcgtcgg ccgcgccggt gccggagacg cccacgctga
     5641 cggtcagctc caggccctcc gggaggccgt actgcgcgcg ctcggcggcg gcatcggggc
     5701 cggcgatggc gcggacgacg cggtcggcgt agcggcggcc gtcctcggcg gaggtatcgg
     5761 gcagcagcac ggcgaactcg tcgccgccgg tgcgcgcggc gacgtcgacg ccgcgcgact
     5821 ggcgggccag cacgccgccg agccaggcca gcacgcggtc gcccatctcg tggccgtgcg
     5881 cgtcgttgac gtccttgaag tggtcgatgt ccaccatcgc catcgccatc ggggtcttct
     5941 ggcggagggc gcggttgacc tcgatcgaca tccgctcctc caggccgcgc cggttcagca
     6001 gcccggtcag cgggtccacc cgggcctgct cctccaggtc gcggaccagg cgggcgttgc
     6061 gcgaggccag gtagcgcact acggcggcca cgacggtgac cgaggccacg acgtcgatcc
     6121 agcggtcggc gctgaccagc tcggcgggca tggtcagcag ggcgacgccc tgggccagcg
     6181 cgacgaaggc gacgatgaac acggcgcccg cgcggccgta gaagtaggac atccacagca
     6241 cgggccacat gtagagcacc gcgccgtcgg aggggccgcc gcgggtctcg gccagcgcgt
     6301 aggcgatgca cgcgacgccg atgggggcga acacggcgag ccacttgcgc ggcatcttcg
     6361 gtcccacggt cacggcgatc gcgcccatga ccacggcggc gatgcccggc acgatcgagt
     6421 tctcggggcc tccggggagg gccgtctcga gggcggcgat ggcgacggcg ccgacgaaga
     6481 tggccgccgc gctgtaggcg atcgtcttcc gctcctcgcg cgcggcgtgt tgggtcggtg
     6541 tgagcatcac tccacgcatc ggccgctgag cgaaagcctt gaacgccggg ctcccgcggc
     6601 ggaccgctgt cgacggtcgg tcggcggtcg ggccgaattg caggcgaatt gcggcgaatt
     6661 tgtccgcgcc cttgacataa accattgtga cgatggatac tcgcgccgcc gtatggggag
     6721 agggacaagc gagccgccgc cgtccgtgcg cgagttgaac aggagccgcg tgatcgaggc
     6781 cctgcgcgag ctcggggtgg ccagccgggc cgacgtggcc cgccacaccg ggctgtcgcg
     6841 gaccaccatc tccggcctgg tcgccgagat gcgcgacgag ggcctggtgg tcgaccggcc
     6901 cggccccgac ggcgacgccc cggccagcgg cgcgggccgc ccgccgaccc cgatcggcct
     6961 gagcgcgcgt gccggcgcgg ccctgggcat cgacttcggc aagcgccacc tcgcggtcgt
     7021 ggtggccgac ctctcgcaca cgctgctggc cgaggaccag cgcgagatgg aggagggcta
     7081 cgactccgcc gccggcatcg acgccgccat cgagctggtg gaggacgtgc tggcccgggc
     7141 cggcatcccg gtccgccagc tgctgggcgt cggcgtcggc ctgcccgggc cggtgcacgg
     7201 cccgtcgggc caggtgggct cgtccgcgat cctccccggc tgggaggggc tgcaggtggc
     7261 gcgcgagatg gagggtcgcc tgggcgtgcc ggtccgcgtg gacaacgacg cgaacctggg
     7321 cgcgctggcc gagctgttct ggggcgccgg ggagggcgcg gccgacctcg tctacctgaa
     7381 ggtggccacc ggcatcggcg ccgggctgat cttccagggc cggctgttcc acggcgcggg
     7441 cggcaccgcc ggcgagatcg gccacctgac catcgacgag cacggcccga tgtgccgctg
     7501 cggcaaccgc ggctgtctgg agaccttcgc cgccggcgac gcgctggtcg agctcctgcg
     7561 ctcgagcctc ggccacgagc tgaccgcgga ggagatcgtg gaccaggcca tcgccggcga
     7621 cgtcgcgtgc cggcgcgcga tcgcggacgc cgggcgccac ctgggccatg ccgtgggcgc
     7681 catctgcaat ctgatcaacc cctcacgcgt cgtggtggga ggcagcatcg gccaggccgg
     7741 cgatctgctc ctcggcgaga tgcgcgaggc ggtcaggcgc cgggcggtgc tgtccgcggc
     7801 gcaggacgtg gaaatcgtgg ccggagtgct gggcgaacgc gccgaggtgc tgggagcggt
     7861 ggctctcgtg ctcggcgacg ccgaggcggc acccgtgccg gaagcagtga cgacgtaaca
     7921 acgaggaggg aaaccccgga tgttcagaac gaccagtagg aaaggcgccg cggcgttcgc
     7981 cgcttgtgcc ctgatcgcag gtggcgtggt ggccggatgt ggcaccagcg acgaggacga
     8041 cagcgggggc ggtgacagcg ggggaggctc gaagagcggc aagatcgcgc tgctgctgcc
     8101 cgagtccaag accgctcggt acgagtcgca ggaccggccc aacttcgaga agaaggtcaa
     8161 ggagctgtgc gccgactgcg agatcatcta cagcaacgcc gaccaggacg ccgccaagca
     8221 gcagcagcag gctgacgcgg ccctgaccaa gggcgccaag gtcatggtgc tggacccggt
     8281 ggacgcggcc tcggccggcg gcatcgtggc ccgggccaaa cagaag
//
LOCUS       DASUPW010000053         6730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1535312, whole genome shotgun sequence.
ACCESSION   DASUPW010000053 DASUPW010000000
VERSION     DASUPW010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535312"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VFY44_01240"
     CDS             <1..525
                     /locus_tag="VFY44_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909083.1"
                     /translation="CVLADGLAAWSLEGPRPVPFAQPLAPCPWQVGNPQVVYQGLPKA
                     WEDDFRDFAAQRGLTLEDPHDWHRRFPSLPLPGEVLAVMRAPSGLRILFPVDVAVHQL
                     RAVRGAIAFEVPEGTPDTAPGGISSAESRTTTAVRAGVAVVWTHRFFGYTREAETLVD
                     DALAAARAAGIEPA"
     gene            529..1263
                     /locus_tag="VFY44_01245"
     CDS             529..1263
                     /locus_tag="VFY44_01245"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5909084.1"
                     /translation="MLAQLSDLHLRPGDPGPEARLERAVAVVAAMDPLPDALLVSGDL
                     ADVPAPEVYARARELLEAAGLPLVVVSGNHDDTGQLGAAFGDDRLEGRAGPLRVVGVD
                     TSRPGQDGGRVEDRDIARLDAVLSADPGTPTVLAMHHPPVPTGVRSLDAIGLPAADRD
                     AVAQLLLRHPQVKTVACGHVHRAMATTLGGAAVVIAPGVSSQLRLDLRPEADHEIEMT
                     TEPAGLMVHLLVGDGIRSHFQVLPDA"
     gene            1256..2335
                     /locus_tag="VFY44_01250"
     CDS             1256..2335
                     /locus_tag="VFY44_01250"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEX5909085.1"
                     /translation="MPDRVLDDRALNRALLARQGLLARRQATPLEMIDRLVGMQGEEP
                     SFPYVALWSRIRDFDPAALEGVLTGREAVRLWVMRGTIHLTTVADAVSMYPFTRRLHG
                     QSFRSAFTAGLAGADPADVTAAAVELISAQPRTKGEIADALQERWPDAERQSLSYCVT
                     HHAPCVQVPPRGLFRQPGPVRLAPLEQWVGRQLDPDPSLTDLVWRYLAAFGPATVADM
                     RIWSRITGLREAFEELRPRLLTYRDKEGRELFDVPDGPLPDPDTPAPPRFLPRLENAL
                     LGFDDRSRVFDGHGPGPQARKGRLAGVVLVDGFHRANWLLSEDKGTATLFVDGLEGAP
                     DEVEAEARAFLDFWAAGADRRELVL"
     gene            complement(2332..2610)
                     /locus_tag="VFY44_01255"
     CDS             complement(2332..2610)
                     /locus_tag="VFY44_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909086.1"
                     /translation="MEATPEHAPDSDEDIRALVARLSRPHPSGGTVIERATILAEGPD
                     FTKIVAWITEHDGRPESAVEAPSGGLHGSRAAPVTGPPLRYVLPAGAL"
     gene            complement(2621..3580)
                     /locus_tag="VFY44_01260"
     CDS             complement(2621..3580)
                     /locus_tag="VFY44_01260"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HEX5909087.1"
                     /translation="MLPVLALDRLDLDELREACHDIGFFYVSGHGVDQELCARVLELS
                     RRFFELPLEERESIDHIHSPQFRGYTRLADERTNGVRDQREQIDLGADREALDLAAGD
                     PAYMRLQGPNLWPPALPELQGAVEPWLDQMGVLGHRLLRAIAASLGLDEDLFEPAFAE
                     LPHVHAKIIRYPGVDSAGGDQGVGAHKDYGFLSLLMLEPGSAGLQAEGPDGTWIDAPP
                     VDGTFLVNLGEMLEVATDGYLRATVHRVVSPPAGSDRISVPYFFNPRLDATVERVRLP
                     PELAAEAEGAAADDTLSETYGVNALRGWVRAHPEVAARHYPSE"
     gene            complement(3580..4326)
                     /locus_tag="VFY44_01265"
     CDS             complement(3580..4326)
                     /locus_tag="VFY44_01265"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX5909088.1"
                     /translation="MIDAPPPPHAAVTPVSPEFTKAAIAPRHAYVAGSPVRLRLRFSS
                     PSGAADLRVEIRRRGRLAKRFVVRGATPPGPLALRWATSNARDGRYVVSGGPDGGLMK
                     RLGSFTLRNGIYPVRGGHGARGGVGLFGAGRNGGRTHEGFDILTPCGTPVVAARGGVV
                     KRRYYDPVLYGNNVIVRGAGQRREYWYSHLRTPGRVAAGGRVRTGQRLGEVGQTGNAR
                     TTPCHLHFEIHGPAGPFDPLPRLRQWDAYS"
     gene            complement(4323..5273)
                     /locus_tag="VFY44_01270"
     CDS             complement(4323..5273)
                     /locus_tag="VFY44_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX5909089.1"
                     /translation="MRGGIDLGGTKIQTAVVDDDGKVLGDARLPTPTEGEPADVAAAM
                     AEAMRQAASAAKVETKDLEGVGVGSPGVVDDAAGTVAGAGNLPHWDAPFALKDALAEA
                     LGSPVHLGNDVQVATDAEVALGAGRPYRSLLGVFWGTGVGGGIVLDGKPWLGRSAAGE
                     IGHVVVKIGGRRCPCGRKGCMEAYAGRGAMERRARKRMDKGEHTELFKIMEKRGRERL
                     SSGVWARALKAGDDMAASLLDEAVEALGAGVASAINVLDVEAVVIGGGMGLRMGKPYV
                     GRIQAAMTPHLFNDSDPPAVVLAELGDLGGAIGASLLART"
     gene            5294..6049
                     /locus_tag="VFY44_01275"
     CDS             5294..6049
                     /locus_tag="VFY44_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018104742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5909090.1"
                     /translation="MTLLEAIAVLLAGGAAGAINVVVGSGTLVTFPVLLAVGYSPLVA
                     NVSNTLGLVPGSLAGAVGYREELRGRGRQVTEFACASVAGGVVGAVLLLVLPEGAFEV
                     VVPVLVAAAVVMVAVQPLVAKRVGAPERGGRKARGGPLLFGLIFVTGVYGGYFGAAQG
                     VLLLGIMGLMLNDSLQELNAIKNVLAGLVNGVAALVFVFAAEPAWEAVGLIAVGATIG
                     GAAGAKVARRLPPNVLRGVVVVVGCVAFVQLVR"
     gene            complement(6046..>6730)
                     /locus_tag="VFY44_01280"
     CDS             complement(6046..>6730)
                     /locus_tag="VFY44_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5909091.1"
                     /translation="WFFIRRRPEDVGLLPDGDSAPAAEDAKPAPEREWTKQEVLPTAW
                     LTAYRMLFVKSGLRPGQTMLVQGASGGVATALTALGSNAGLRVWVTGSTEQKRAIAEA
                     QGADATFETGARLPERVDAVFETVGGATWSHSLKSLKPGGTVVIAGATSGDADKGELQ
                     RLFFLQLNVVGSTMGTRDELADLLAYLEREEITPRIGAEVALADAAEGVKAMIDGDTA
                     GKIVFTVEG"
BASE COUNT          776 a         2510 c         2537 g          907 t
ORIGIN      
        1 tgcgtgctgg ccgacggcct ggccgcctgg tccctggagg gcccccggcc ggtcccgttc
       61 gcgcagcccc tggccccctg cccgtggcag gtcggcaacc cgcaggtggt ctaccagggg
      121 ctgcccaagg cctgggagga cgacttccgc gacttcgccg cccagcgcgg gctgaccctg
      181 gaggacccgc acgactggca ccggcggttc ccctcgctac cgctgcccgg tgaggtgctg
      241 gccgtgatgc gcgcgccatc ggggctgcgg atcctgttcc cggtcgacgt ggcggtccac
      301 cagctgcgcg cggtgcgcgg cgcgatcgcg ttcgaggtgc ccgagggcac gcccgacacg
      361 gcccccggcg ggatctcctc ggccgagtcc cggaccacca ccgccgtgcg cgccggggtg
      421 gccgtggtgt ggacccaccg attcttcggc tacacccgcg aggcggagac gctggtggac
      481 gacgcgctcg cggccgcgcg cgcggcgggg atcgaacccg cctgacccgt gctggcccag
      541 ctgtccgacc tgcacctgcg cccgggtgac ccgggccccg aggcgcgcct ggagcgggcg
      601 gtggcggtgg tggccgcgat ggacccgctc cccgatgcgc tcctggtcag cggcgacctg
      661 gccgacgtgc ccgcgcccga ggtctacgcg cgcgcccgcg agctgctgga agccgccggg
      721 ctcccgctgg tggtggtcag cgggaaccac gacgacaccg gtcagctcgg cgccgcgttc
      781 ggcgacgacc ggctggaggg ccgggccggc ccgctgcggg tggtcggcgt ggacaccagc
      841 cggccgggcc aggacggcgg acgggtcgag gaccgggaca tcgcgcggct ggacgccgtg
      901 ctgtccgccg accccgggac gcccaccgtg ctggccatgc accacccgcc cgtgccgacg
      961 ggcgtccgct cgctggacgc catcggcctg cccgccgccg accgcgacgc cgtggcgcag
     1021 ctgctgctcc gccaccccca ggtgaagacg gtggcgtgcg ggcacgtgca ccgggccatg
     1081 gccacgacgc tcggcggcgc ggcggtggtc atcgcgccgg gcgtcagcag ccagctacgg
     1141 ctggacctgc gcccggaggc ggaccacgag atcgagatga ccaccgagcc cgcggggctg
     1201 atggtccatc tcctggtggg cgacgggatc cggtcgcact tccaggttct gcccgatgcc
     1261 tgaccgggtc ctggacgacc gcgcgctgaa ccgcgccctg ctggcccgcc aggggctgct
     1321 ggcgcgccgg caggcgacgc cgctggagat gatcgaccgg ctggtgggca tgcagggcga
     1381 ggagccgtcc ttcccgtacg tggcgctgtg gtcgcgcatc cgggacttcg acccggcggc
     1441 gctggagggc gtgctgaccg ggcgggaggc ggtgcggctg tgggtcatgc gcggcaccat
     1501 ccacctgacc acggtcgcgg acgcggtctc gatgtacccg ttcacccgcc gcctgcacgg
     1561 ccagtccttc cgcagcgcct tcaccgccgg cctggccggt gccgaccccg ccgacgtcac
     1621 ggcggccgcc gtggagctga tcagcgcgca gccacggacg aagggcgaga tcgcggacgc
     1681 gctgcaggag cgctggcccg acgccgagcg gcagtcgctg agctactgcg tgacccacca
     1741 cgcgccgtgc gtgcaggtgc cgccccgcgg cttgttcaga cagcccggcc cggtgcggct
     1801 ggcgccgctg gagcagtggg tggggcgtca gctggacccg gacccgtcgc tcaccgatct
     1861 ggtgtggcgc tacctggcgg cgttcggccc cgcgacggtg gccgacatgc gcatctggtc
     1921 gcgcatcacc gggctgcgcg aggcgttcga ggagctgcgc ccgcggctac tgacgtaccg
     1981 cgacaaggag ggacgcgagc tgttcgacgt ccccgacggg ccgctgcccg acccggacac
     2041 gcccgcgccg ccgcggttcc tgccgcggct ggagaacgcg ctgctgggct tcgacgaccg
     2101 ctcgcgtgtg ttcgacggcc acggcccggg accgcaggcc cgcaagggcc gcctggccgg
     2161 cgtggtgctg gtcgacggct tccaccgggc caactggctg ctgtccgagg acaagggcac
     2221 ggccacgctg ttcgtggacg ggctggaggg cgccccggac gaggtcgagg ccgaggctcg
     2281 cgcgttcctg gacttctggg cggcgggcgc cgaccggcgc gagctcgtgc tctagagcgc
     2341 gccggcgggc aggacgtagc gcagcggcgg cccggttacg ggcgcggcgc ggctgccgtg
     2401 cagcccgccc gacggtgcct cgacggcgga ctcgggccgg ccgtcgtgct cggtgatcca
     2461 ggccacgatc ttcgtgaagt cggggccctc ggccaggatc gtggcgcgct cgatcacggt
     2521 gcccccggac gggtgcggcc gggacaggcg cgcgaccagg gcgcggatgt cctcgtcgga
     2581 gtcgggtgcg tgttcggggg tggcttccac cccgtcgaag ctactccgat gggtagtggc
     2641 gcgcagccac ctcgggatgc gcgcgcaccc agccgcgcaa cgcgttcacc ccgtaggtct
     2701 ccgacagcgt gtcgtcggcg gccgcgccct cggcctccgc cgccagctcc gggggcagcc
     2761 gcacccgctc gacggtggcg tccagccgcg ggttgaagaa gtagggcacc gagatccggt
     2821 cgctgccggc cggcgggctg accacccggt gcacggtggc gcgcaggtag ccgtcggtgg
     2881 ccacctcgag catctcgccc aggttcacca ggaacgtccc gtccaccggc ggggcgtcga
     2941 tccaggtgcc gtcaggtccc tcggcctgga gcccggccga gccgggctcc agcatcagca
     3001 gcgacaggaa gccgtagtcc ttgtgtgcgc ccacgccctg gtcgcccccg gcggagtcga
     3061 cccccgggta gcggatgatc ttggcgtgca cgtggggcag ctcggcgaac gcgggctcga
     3121 acaggtcctc gtccagcccc agggaggcgg cgatggcgcg cagcagccgg tgccccagca
     3181 cgcccatctg gtccagccac ggctccacgg cgccctgcag ctccgggagc gcgggcggcc
     3241 acaggttcgg cccctggagg cgcatgtagg ccgggtcgcc ggccgccagg tcgagcgcct
     3301 cgcggtcggc gcccagatcg atctgctcgc gctggtcgcg caccccgttg gtgcgctcgt
     3361 cggccagccg cgtgtagccg cggaactgcg gcgagtggat gtggtcgatg ctctcgcgct
     3421 cctccagcgg cagctcgaag aagcggcgcg agagctcgag cacccgcgcg cagagctcct
     3481 ggtcgacgcc gtgcccggac acgtagaaga acccgatgtc gtggcaggcc tcgcgcagct
     3541 cgtcgaggtc cagccggtcg agtgccagta ccgggagcat cagctgtacg cgtcccactg
     3601 acgcaggcgc ggcagcgggt cgaacggccc ggcggggccg tggatctcga agtgcaggtg
     3661 gcagggggtg gtgcgggcgt tgccggtctg gcccacctcg cccaggcgct ggccggtccg
     3721 cacgcgcccg ccggccgcga cccggcccgg tgtgcgcagg tgcgagtacc agtactcgcg
     3781 ccgctgcccg gcgccgcgga cgatcacgtt gttgccgtac agcacggggt cgtagtagcg
     3841 ccgcttgacc acgccgccgc gggcggccac gaccggcgtg ccgcacgggg tcaggatgtc
     3901 gaagccctcg tgggtgcggc cgccgttgcg cccggcgccg aacaggccca cgccgccgcg
     3961 ggcgccgtgg ccaccgcgca ccgggtagat gccgttgcgc agcgtgaagc tccccaggcg
     4021 cttcatcagc ccgccgtcgg gaccgccgct gaccacgtag cgcccgtcgc gcgcgttgct
     4081 cgtcgcccag cgcagcgcca gcggccccgg cggcgtggcc ccgcggacca cgaagcgctt
     4141 ggccagccgg ccgcggcggc ggatctccac gcgcaggtcg gccgcgccgg agggcgagct
     4201 gaagcgcagc cgcaggcgga ccgggctccc ggcaacgtac gcgtgccgcg gcgcgatggc
     4261 ggccttggtg aactcgggcg acaccggcgt gacggccgcg tggggcggcg gcggcgcgtc
     4321 gatcacgtgc gcgcgagcag cgaggccccg atggcgccgc ccaggtcgcc cagctcggcc
     4381 agcaccaccg ccggcgggtc cgagtcgttg aacaggtgcg gggtcatggc cgcctggatc
     4441 cggcccacgt agggcttgcc catccgcagg cccatgccgc cgccgatgac cacggcctcc
     4501 acgtccagca cgttgatcgc cgaggccacg ccggcgccca gcgcctcgac ggcctcgtcc
     4561 agcagcgagg cggccatgtc gtcgccggcc ttcagcgcgc gcgcccagac cccgctggac
     4621 agccgctcac ggccgcgctt ctccatgatc ttgaacagct cggtgtgctc gcccttgtcc
     4681 atgcgcttgc gcgcgcgccg ctccatggcg ccgcggccgg cgtaggcctc catgcagccc
     4741 ttgcgcccgc acgggcagcg ccgtccgccg atcttgacca ccacgtggcc gatctcgccg
     4801 gcggccgagc ggcccagcca cggcttgccg tccagcacga tcccgccgcc cacgccggtg
     4861 ccccagaaca cgccgagcag ggagcggtag ggccggccgg cgcccagcgc cacctcggcg
     4921 tcggtggcca cctggacgtc gttgcccaga tgcacgggcg agcccagcgc ctcggccagc
     4981 gcgtccttga gcgcgaacgg cgcgtcccag tggggcaggt tgccggcgcc cgcgacggtg
     5041 ccggccgcgt cgtccaccac gcccggcgag cccacgccga cgccctcgag gtccttcgtc
     5101 tccaccttcg cggcggacgc ggcctggcgc atggcctcgg ccatggccgc cgcgacgtcc
     5161 gccggctcgc cctcggtggg cgtcggcagg cgcgcgtcgc cgaggacctt gccgtcgtcg
     5221 tccaccaccg cggtctggat cttggtgccg cccaggtcga tgccgccgcg catggcggtc
     5281 atgctacgcg gcgatgacgc tgctcgaagc catcgcggtg ctcctggcgg ggggcgcggc
     5341 cggagccatc aacgtggtcg tgggctcggg cacgctggtc accttccccg tgctgctggc
     5401 ggtcggctac tcgccgctgg tggccaacgt gtccaacacg cttggcctgg tgcccggctc
     5461 gctggccggc gccgtcgggt accgcgagga gttgcgcggg cgcggccgcc aggtcaccga
     5521 gttcgcctgc gcgtcggtcg cggggggcgt cgtgggcgcc gtgctcctgc tggtgctgcc
     5581 cgagggcgcg ttcgaggtcg tggtgccggt gctggtggcg gcggccgtgg tcatggtggc
     5641 cgtccagccg ctggtggcca agcgggtcgg ggcccccgag cgcggcgggc gcaaggcccg
     5701 cggcggcccg ctgctgttcg gcctgatctt cgtgaccggc gtctacggcg gctacttcgg
     5761 cgccgcccag ggcgtgctgc tgctgggaat catggggctg atgctcaacg actccctcca
     5821 ggagctgaac gccatcaaga acgtgctggc cgggctggtc aacggcgtgg ccgcgctggt
     5881 gttcgtcttc gcggccgagc cggcctggga ggcggtgggc ctgatcgccg tgggggcgac
     5941 gatcggcggg gccgcgggcg ccaaggtggc gcgccggcta ccgccgaacg tgttgcgcgg
     6001 cgtggtcgtg gtggtcggct gcgtggcgtt cgtgcagctg gtccgttagc cctcgacggt
     6061 gaagacgatc ttgccggcgg tgtcgccgtc gatcatcgcc ttcacgccct ccgccgcatc
     6121 ggccagggcc acctccgcgc cgatgcgcgg cgtgatctcc tcgcgctcca ggtaggccag
     6181 caggtcggcc agctcgtcgc gcgtgcccat cgtggagccg accacgttca gctggaggaa
     6241 gaacagccgc tggagctcgc ccttgtcggc gtcgcccgac gtggcgccgg cgatgaccac
     6301 cgtgccgccc ggcttcagcg acttcagcga gtgcgaccag gtggcgccgc ccacggtctc
     6361 gaacaccgcg tccacgcgct cgggcagtcg cgcccctgtc tcgaaggtgg cgtccgcgcc
     6421 ctgggcctcg gcgatcgccc gcttctgctc ggtcgacccg gtcacccaca cgcggaggcc
     6481 cgcgttcgag cccagcgccg tcagcgcggt ggccacgccg ccggacgcgc cctgcaccag
     6541 catcgtctgg cccggccgca gcccggactt cacgaacagc atccggtagg cggtcagcca
     6601 ggcggtgggg aggacttcct gcttcgtcca ttcccgctcg ggcgccggtt tcgcgtcttc
     6661 ggcggcgggc gctgaatcgc cgtcgggcag cagtccgacg tcttccggcc ggcggcggat
     6721 gaagaaccag
//
LOCUS       DASUPW010000054         4075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1119946, whole genome shotgun sequence.
ACCESSION   DASUPW010000054 DASUPW010000000
VERSION     DASUPW010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119946"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VFY44_01285"
     CDS             <1..709
                     /locus_tag="VFY44_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HEX5909092.1"
                     /translation="HTTARCTVAPRVALLTTGDELTAPGTPLAPGGIYSSNGPALAAL
                     VQDAGGRVVLAEDVPDTAEGTRSALARAIEAADVVCVSGGVSVGPHDHVKGAFADQGM
                     EERFWRVALKPGKPTWFGSAGPKLGFGLPGNPVSALVTFLLFARPALLALQGTDPAIA
                     RITGRLTAPVTPEAGRTNAVRCLLRAGAEGWELTPTGPQGSHQLTSMLAAGALALIEP
                     GEDELPAGTRVRAELLP"
     gene            726..1430
                     /locus_tag="VFY44_01290"
     CDS             726..1430
                     /locus_tag="VFY44_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909093.1"
                     /translation="MRKRDPRRVIGRRREARARVKIIDVPESGSSADGSVSTKQEAEV
                     TLPRSELDKVWNAEYLERLARTYWRFLTRVSLGILRVVYTDEGREVCLFGKPFTLLTF
                     HPPDYETEGDCGSVTWRIKQGLLVAPTGRTKGYLKLTVQRPPADGQEHPGTDLITGTV
                     TSEVANFYPLIAGWGWFARFGQHIYRITQLRIHIIVTHAFLRSLARLDLAPSVVGALK
                     AQADESVQKEEEPAAR"
     gene            complement(1427..2431)
                     /gene="glpX"
                     /locus_tag="VFY44_01295"
     CDS             complement(1427..2431)
                     /gene="glpX"
                     /locus_tag="VFY44_01295"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX5909094.1"
                     /translation="MNDAPKPDRNLALELVRTTEYAALASARWIGRGQKESADGAAVD
                     AMRLMLDTVSMRGTVVIGEGEKDEAPMLYNGEEVGDGSGPEVDVAVDPLEGTELCANG
                     MPNSLATIAVAEKGAMFDPGPNVYMMKMATSDSYAHLLDLDRPIGETLKLMADEKGDD
                     IGDLVVVMLDRPRHEQMIADIREAGARIRLIPHGDVSAALLAVTEDSAADLLYGIGGT
                     PEGVLSAAAIQCLGGKIIGRVWPRDDDEKQAALDAGYDLDEVLDTDRLCSPGDTFFSA
                     TGVTDGDSLRGVHYEGTGARRGATTESLVMRSRSGTVRRVSARHDRQKLRDITAGAIS
                     "
     gene            complement(2453..2962)
                     /locus_tag="VFY44_01300"
     CDS             complement(2453..2962)
                     /locus_tag="VFY44_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909095.1"
                     /translation="MEASRAETGYLAPSRLTFGELAGCIAALVFALSLFLPWYGTSES
                     NPNSQLDLVGGGTAKGGETVSAWQMFPILRWILLAAVIAPFILAWIVMRRHKLEWKPG
                     EITMIVGAAAFVLVLCQGVILGRPGESVEISLEWGYPVALLACIGMAVSGFLRQSRHV
                     DAKKPPGTI"
     gene            3057..>4075
                     /locus_tag="VFY44_01305"
     CDS             3057..>4075
                     /locus_tag="VFY44_01305"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX5909096.1"
                     /translation="MHPHSSKTLPRGGADSLAKGRFRDALAEGRARTLKLVEPVETED
                     LDRVHDPLMSPLVWDLGHIAAFEDLWLGQRAGGLEPLRPDLAQVYDASETPRAERGDI
                     PYLRRDDAIAYMDAVRERSLAVLDRADLSADSDRLNANGFVWEMLVRHEHQHNETMLQ
                     TLQLARPGTYAPTPRPGPGDRRDGEHHAGTVHIPEGEFEMGAATAGFAYDNERPRHRV
                     HLHAYDIDREPVTNGSYAEFVADGGYDARELWSEEGWRWLVDENVDRPLYWTADGAER
                     RFDRVAQIDPSLPVMHVSWYEADAFARWAGGRLPTEAEWERAAAWDPVTGEPLPFPWG
                     HEPPA"
BASE COUNT          561 a         1492 c         1473 g          549 t
ORIGIN      
        1 ccacaccacg gcccgctgca cggtggcgcc ccgcgtggcg ctcctgacca ccggcgacga
       61 gctgaccgcg ccgggcacgc cgctggcccc cggaggcatc tacagctcca acgggccggc
      121 gctggcggcg ctggtgcagg acgccggcgg ccgcgtggtg ctggccgagg acgtgcccga
      181 cacggcggag ggcacccggt cggcgctggc gcgggcgatc gaggcggccg acgtggtgtg
      241 cgtgtcgggc ggggtgtccg tgggtcccca cgaccacgtc aagggcgcgt tcgccgacca
      301 gggcatggag gagcggttct ggcgcgtggc cctgaagccc ggcaagccca cgtggttcgg
      361 ctcggcgggc ccgaagctgg gcttcggcct gcccggcaac ccggtgtccg cgctggtgac
      421 cttcctgctg ttcgcccgcc ccgcgctgct ggcgctgcag ggcaccgacc ccgcaatcgc
      481 ccggatcacg ggccgcctga ccgcgcccgt gacgccggag gcgggccgga ccaacgccgt
      541 gcgctgcctg ctgcgcgccg gcgccgaggg ctgggagctg acccccaccg gcccgcaggg
      601 ctcccatcag ctgacctcga tgctggccgc gggggcgctg gcgctgatcg agccgggcga
      661 ggacgagctg cccgccggca cccgggtccg cgccgagctg ctcccgtgaa tatcgtctgc
      721 ggaccgtgag gaagcgtgac cccagacgag tgatcggccg ccgccgcgaa gcccgcgcgc
      781 gcgtcaagat catcgatgtg cccgagtccg gctcgtcggc ggacgggagc gtgagcacca
      841 agcaggaggc cgaggtgacc ctgccccgct ccgagctgga caaggtctgg aacgccgagt
      901 acctggagcg cctggcccgc acctactggc gcttcctcac gcgcgtctcg ctggggatcc
      961 tccgcgtcgt gtacaccgac gaggggcgcg aggtgtgtct gttcggcaag cccttcaccc
     1021 tgctgacctt ccatccgccg gactacgaga ccgagggcga ctgcgggtcg gtgacctggc
     1081 ggatcaagca gggcctgctg gtggcgccca ccgggcgcac caaggggtac ctcaagctca
     1141 ccgtccagcg cccgcccgcc gacggccagg agcatcccgg caccgacctg atcacgggca
     1201 ccgtgacctc cgaggtggcc aacttctacc cgctgatcgc cgggtggggc tggttcgccc
     1261 gcttcggcca gcacatctac cggatcaccc agctgcggat ccacatcatc gtgacccacg
     1321 ccttcctgcg ctcgctggcg cggctggacc tggcgccgtc ggtggtcggg gcgctcaagg
     1381 cccaggccga cgagtccgta cagaaggaag aggagccggc ggcgcgctag ctgattgcgc
     1441 cggcggtgat gtccctgagc ttctgacggt cgtggcgggc gctgacccgc cgcaccgtgc
     1501 ccgagcgcga gcgcatgacc agcgactcgg tggtcgcccc gcggcgcgcg cccgtgccct
     1561 cgtagtgcac gccgcgcagg gagtcgccgt cggtgacgcc ggtggcggag aagaacgtgt
     1621 cgcccggtga acacagccgg tcggtgtcca ggacctcgtc caggtcgtag ccggcgtcca
     1681 gggcggcctg cttctcgtcg tcgtcgcggg gccacacgcg gccgatgatc ttgccgccaa
     1741 ggcactggat ggccgcggcg gagagcacgc cctcgggcgt gccgccgatg ccgtagagca
     1801 ggtccgcggc cgagtcctcg gtgaccgcca gcagcgcggc cgagacatcc ccgtgcggga
     1861 tcagccggat gcgggcgccg gcctcgcgga tgtcggcgat catctgctcg tggcggggcc
     1921 ggtcgagcat gaccacgacc aggtcgccga tgtcgtcgcc cttctcgtcg gccatgagct
     1981 tgagggtctc cccgatcggc cggtccaggt ccagcaggtg cgcgtaggag tccgaggtgg
     2041 ccatcttcat catgtagacg ttggggcccg ggtcgaacat ggcgcccttc tcggccacgg
     2101 cgatggtggc cagcgagttg ggcatgccgt tggcgcacag ctcggtgccc tccagcgggt
     2161 cgacggccac gtcgacctcg gggccgctgc cgtcgccgac ctcctcgccg ttgtagagca
     2221 tcggcgcctc gtccttctcg ccctcgccga tgacgacggt cccgcgcatg gagaccgtgt
     2281 ccagcatcag gcgcatggcg tcgacggcgg cgccgtcggc cgactccttc tggccccgcc
     2341 cgatccagcg agccgacgcc agcgcggcgt actcggtcgt gcgcaccagc tccagcgcga
     2401 ggttacggtc cggtttgggg gcgtcgttca cgccggaagt tctaccggac gcctagatgg
     2461 tgccgggcgg cttcttggcg tccacgtgcc tggattggcg caggaagccg ctgaccgcca
     2521 tgccgatgca ggccaggagc gcgaccggat agccccactc gagggagatc tcgacgctct
     2581 cgccgggccg gcccaggatc acgccctggc acaggacgag cacgaacgcc gcggcgccga
     2641 cgatcatcgt gatctcgccc ggcttccact cgagcttgtg ccggcgcatg acgatccagg
     2701 ccaggatgaa gggcgcgatc accgcggcca gcaggatcca gcgcaggatc gggaacatct
     2761 gccacgcgct gacggtctcg ccgcccttgg cggtgccgcc gccgaccagg tcgagctgcg
     2821 agttggggtt gctctcgctg gtgccgtacc agggcaggaa cagcgacagc gcgaacacga
     2881 gcgccgctat gcatccggcc agctcgccga acgtcagcct gctcggcgcc aggtacccgg
     2941 tctccgccct cgaagcttcc accggcctca acgtaccacc gggacgccgg ccgtgggccg
     3001 ctccgctgcg cgggttggcg ccccggtacc ctggtcggag caaccggtag ccgcccgtgc
     3061 accctcatag ctcgaaaaca ctgccccgtg gaggggccga ctcgctggcc aagggcaggt
     3121 tccgtgacgc gctggccgag ggccgcgcgc ggacgctgaa gctggtcgag ccggtggaga
     3181 ccgaggacct ggaccgggtg cacgaccccc tgatgagccc gctggtgtgg gacctgggcc
     3241 acatcgccgc gttcgaggac ctgtggctgg gccagcgcgc cggaggcctc gagccgctgc
     3301 gccccgacct ggcccaggtc tacgacgcca gcgagacccc gcgcgccgag cgcggggaca
     3361 tcccctacct gcgccgcgac gacgccatcg cctacatgga cgcggtccgt gagcgctcgc
     3421 tggccgtgct ggaccgcgcc gacctgtcgg ccgactcgga ccgcctgaac gccaacgggt
     3481 tcgtgtggga gatgctggtc cgccacgagc accagcacaa cgagacgatg ctgcagaccc
     3541 tgcagctggc ccggccgggc acctacgccc ccaccccgcg gccggggccg ggggaccgcc
     3601 gggacggcga gcaccacgcg ggcacggtgc acatccccga gggcgagttc gagatgggcg
     3661 ccgccacggc cggcttcgcc tacgacaacg agcgcccgcg ccaccgcgtg cacctgcacg
     3721 cctacgacat cgaccgcgag cccgtgacca acgggtccta cgccgagttc gtggccgacg
     3781 gcggctacga cgcgcgcgag ctgtggagcg aggagggctg gcgctggctg gtggacgaga
     3841 acgtggaccg gcccctgtac tggaccgccg acggcgccga gcgccgcttc gaccgggtgg
     3901 cccagatcga tccgagcctg cccgtgatgc acgtgtcctg gtacgaggcc gacgcgttcg
     3961 cgcgctgggc cggcgggcgc ctgccgacgg aggccgagtg ggagcgcgcg gcggcctggg
     4021 accccgtcac cggcgagccg ctgcccttcc cctgggggca cgagccgccc gccta
//
LOCUS       DASUPW010000055         3259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_150875, whole genome shotgun sequence.
ACCESSION   DASUPW010000055 DASUPW010000000
VERSION     DASUPW010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150875"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VFY44_01310"
     CDS             <1..615
                     /locus_tag="VFY44_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909097.1"
                     /translation="LSSKEEFDAWVGGGRNFDPSRPYDQSPLGYSDGAQFSYPGEPMP
                     VPAWKSPSYGIGFDAPDYPAILDRNRNPQRTDDERDADHDGLSNYIETTGPGHASWWS
                     AYLAGENEPAWPGTVREPGGTYTYWGEFTRRPFADVDLADPDVDGDTLLDGEDDQDND
                     DWNNLTEMYNYRTVDTPAGPRRTNAFNPCAPDPESRTCPLYFPL"
     gene            640..1803
                     /gene="aroC"
                     /locus_tag="VFY44_01315"
     CDS             640..1803
                     /gene="aroC"
                     /locus_tag="VFY44_01315"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX5909098.1"
                     /translation="MAFRFTTAGESHGPGLTAVIEGLPAGLELTADDIDSDLARRQLG
                     HGRGGRMKIEKDRADVTSGVRHGKTLGGPIALKMANKDYANWEERMNPWPVEADVAEV
                     HLPRPGHADLAGVQKYGFTDVRNVLERASARETTARVGVGALAKVYLRALGVEIRSHV
                     TQIGSVTVPYRDDLTVADFDAVDESPVRCLDADGSQAMVEHINTARKANESLGGVYEV
                     IAFGVTPGIGSYVAWDERLDGRLAQAIMSIQAMKGVGIGDGFDLAGRPGSQAHDEIFY
                     SEEGGFARETNRAGGLEGGMTTGTPLVVRGAMKPLPTLTKPLRSVDLETHEPAAALRE
                     RTDSCTVPAAAVVAEAMMALVLAPAYKEKLGGDHIDDARAALRAYEERIGWRR"
     gene            1791..>3259
                     /locus_tag="VFY44_01320"
     CDS             1791..>3259
                     /locus_tag="VFY44_01320"
                     /inference="COORDINATES: protein motif:HMM:NF013887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional shikimate kinase/3-dehydroquinate
                     synthase"
                     /protein_id="HEX5909099.1"
                     /translation="MEALGPQGALVFIGFMGAGKSSGARTVAAELGVQPLDSDREIEA
                     ELGETIEAFFDREGEQAFREREEAAVLRLLGRAGGGAVALGGGSLLSERVRDALASHT
                     VVHLEVAPEEAWRRAAGKGRPLARDRSRFDQLHADRAGLYESIAHATIPPTGREAVRR
                     ALPALRALHRARSAGATGLRMLWAPTRSRDYPVFFGRGAMSAVPIHPVDGRTFAVTDA
                     NVGAHHRVEAEASVVIAPGEANKTLATAENVLSQLARAGARKGDVITAVGGGVVGDLA
                     GLCAALYQRGLRYVQVPTTLVAQVDSAYGGKTGVDLPEGKNYAGVYHQPTAVVCDPAV
                     LDTLPEAEKAAGYAEVVKTALIAGGPLWARVRGGGPVDDETILGCIRTKLDVVAADER
                     DEGRRQVLNLGHTVGHAIEAATGYSRYRHGEAVAVGLLAALRLSGREVLREEVAALLS
                     GHGLPLTFEGASVEQVVALTARDKKRSGDTVPFVLVQAPG"
BASE COUNT          476 a         1170 c         1219 g          394 t
ORIGIN      
        1 ctgagctcca aggaggagtt cgacgcctgg gtgggcggcg gacgcaactt cgacccttcg
       61 cgcccatacg accagtcgcc gctgggctac agcgacggtg cccagttcag ctaccccggc
      121 gagccgatgc ccgtccccgc atggaagagc ccctcctacg ggatcggctt cgatgcgccc
      181 gactatcccg ccatcctcga ccgcaaccgc aacccgcagc ggaccgacga cgagcgcgac
      241 gccgaccacg acggcctgag caactacatc gagaccaccg gtcccggcca cgcctcctgg
      301 tggagcgcgt acctggcggg ggagaatgag ccggcatggc ccggaaccgt cagggagccc
      361 ggcggcacct acacctactg gggcgagttc acccgccgtc ccttcgcgga cgtggacctg
      421 gccgatcccg acgtggacgg cgacaccctg ctggacggcg aggacgacca ggacaacgac
      481 gactggaaca acctcaccga gatgtacaac taccgcacgg tggacacccc cgcgggaccg
      541 cggcgcacga acgcgttcaa tccatgcgct cccgaccccg agtcgcgcac gtgcccgctg
      601 tacttcccgc tgtagcccgc cagtaagctg gcgggccgca tggccttccg cttcaccaca
      661 gccggcgaat cgcacggtcc cggcctgacg gccgtgatcg agggcctgcc cgcgggcctg
      721 gagctgaccg ccgacgacat cgactccgac ctggcccggc ggcagctggg ccacggccgt
      781 ggtgggcgca tgaagatcga gaaggatcgc gccgacgtca cgtccggtgt gcgccacggc
      841 aagaccctcg gcgggccgat cgcgctgaag atggcgaaca aggactacgc caactgggaa
      901 gagcgcatga acccgtggcc ggtggaggcc gacgtggccg aggtgcacct gccccggccc
      961 ggccacgccg acctggcggg cgtgcagaag tacggcttca ccgacgtgcg caacgtgctc
     1021 gagcgggcca gcgcgcgcga gaccaccgcg cgggtgggcg tgggcgccct ggccaaggtc
     1081 tacctgcgcg cgctgggcgt ggagatccgc agccacgtga cccagatcgg gtcggtcacg
     1141 gtgccctacc gcgacgacct gaccgtggcc gacttcgacg cggtcgacga gtcgcccgtc
     1201 cgctgcctgg acgccgacgg ttcgcaggcc atggtcgagc acatcaacac ggcccggaag
     1261 gccaacgagt cgctgggcgg ggtgtacgag gtgatcgcct tcggcgtgac gccggggatc
     1321 ggctcctacg tggcctggga cgagcggctg gacgggcgcc tggcccaggc gatcatgtcg
     1381 atccaggcca tgaagggcgt gggcatcggc gacggcttcg acctggccgg ccggcccggc
     1441 tcacaggccc acgacgagat cttctacagc gaggagggcg gcttcgcgcg cgagaccaac
     1501 cgcgccggcg ggctggaggg cggcatgacc accggcacgc cgctggtggt ccgcggcgcc
     1561 atgaagccgc tgcccacgct gaccaagccg ctgcgcagcg tcgacctaga gacgcacgag
     1621 cccgcggcgg cgctgcgcga gcgcaccgac tcgtgcacgg tcccggccgc cgccgtggtg
     1681 gccgaggcga tgatggcgct ggtgcttgcg cccgcctaca aggagaagct gggcggtgac
     1741 cacatcgacg acgcccgggc cgccctgcgc gcttatgagg agcgcatcgg atggaggcgc
     1801 taggccccca gggggcgttg gtcttcatcg ggttcatggg cgccggcaag tccagcggcg
     1861 cccgcacggt ggcggccgag ctgggggtgc agccgctgga ctccgaccgg gagatcgagg
     1921 ccgagctggg ggagaccatc gaggcgttct tcgaccgcga gggcgagcag gcgttccgcg
     1981 agcgcgagga ggcggccgtg ctgcgcctgc tgggccgcgc cggcggcggg gccgtggccc
     2041 tgggcggcgg ctcgctgctg tccgagcgcg tgcgcgacgc cctggcctcg cacacggtcg
     2101 tgcacctcga ggtggcgccc gaggaggcgt ggcggcgcgc cgcgggcaag gggcgcccgc
     2161 tggcccgcga ccggagccgc ttcgaccagc tccacgcgga ccgcgcgggc ctgtacgagt
     2221 cgatcgccca cgccacgatc ccgccgacgg gccgcgaggc ggtccgccgt gccctgcccg
     2281 cgctgagggc gctgcaccgc gcgcgatcgg ccggggccac cggcctgcgg atgctgtggg
     2341 cgcccacccg ctcgcgcgac taccccgtgt tcttcggccg cggcgcgatg agcgccgtgc
     2401 ccatccaccc ggtggacggc aggacgttcg cggtcaccga cgccaacgtg ggcgcccacc
     2461 accgcgtcga ggccgaggcc agcgtggtca tcgccccggg cgaggccaac aagacgctgg
     2521 ccaccgccga gaacgtgctc agccagctgg cgcgggccgg cgcccgcaag ggcgacgtga
     2581 tcaccgccgt cggcggcggg gtggttggcg acctggcggg actgtgcgcc gcgctctacc
     2641 agcgcgggct gcgctacgtc caggtgccca cgacgctcgt cgcccaggtg gactcggcct
     2701 acggcgggaa gaccggcgtc gacctgcccg agggcaagaa ctacgcgggc gtctaccacc
     2761 agccgaccgc cgtcgtgtgc gatccggcgg tgctggacac gctgccggag gccgagaagg
     2821 cggcgggata cgctgaggtg gtgaagaccg ccctgatcgc cggcgggccg ctgtgggcgc
     2881 gcgtccgggg cggaggcccc gtggacgacg agacgatcct gggctgcatc cgcaccaagc
     2941 tcgacgtggt ggccgccgac gagcgcgacg agggccggcg ccaggtcctg aacctgggtc
     3001 acaccgttgg ccacgcgatc gaggccgcga ccggctactc gcgctaccgc cacggtgagg
     3061 cggtggcggt ggggctgctg gccgccctgc gcctgtcggg ccgcgaggtg ctgcgcgagg
     3121 aggtggcggc gctgctgtcc gggcacgggt tgccgctgac cttcgagggc gcctcggtgg
     3181 agcaggtggt cgcgctgacc gcgcgcgaca agaagcgctc cggcgacacc gtcccgttcg
     3241 tgctcgtgca ggccccggg
//
LOCUS       DASUPW010000056         3519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1536292, whole genome shotgun sequence.
ACCESSION   DASUPW010000056 DASUPW010000000
VERSION     DASUPW010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536292"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..325
                     /locus_tag="VFY44_01325"
     CDS             134..325
                     /locus_tag="VFY44_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909100.1"
                     /translation="MEETTVDLHLQLHVEGERLHGHLGREEDEPREFSGWLGFVSALD
                     ALIAEATAPTNGSQPRRTT"
     gene            322..1701
                     /locus_tag="VFY44_01330"
     CDS             322..1701
                     /locus_tag="VFY44_01330"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5909101.1"
                     /translation="MTVAAPPADHVRALRSRMTGTALAPGDTGYDEARVAFNLIVQQT
                     PPLIAFPADADDVGQVVRFARERGFRVAPQRTGHCAAPIETLDQTILLRTDPLGGVQV
                     DAAARRARVGGGVQWQDVVPVAAESGLSALHGSAADIGVAGYSLGGGMGWQARKHGLQ
                     ANSVTAVELITAGGELVRADRDNEPELFWALRGGGGNFGVVTALEFDLYPLETVFAGM
                     MFFGLEQAPEVFHTWRELLAGVPDELTSTCRILRFPDIPELPDVVRGQNVVVVNGAYL
                     GAEAEGRDLLAPLADLGPGMDTWGMVPAAALTELHMDPPDPVPAQTDHLLVDLSAEGF
                     DRMLAATGPGVDTPLGMVELRHTGGALGRSEPGHGALDHLPGSYSAFAGGMVVDAESA
                     AAVGGDLDRWRASLAPYRAGEYLNFVERPTDVAAAYGEGRYERLARVKAAFDPDEVFR
                     ANHGIRPRA"
     gene            complement(1711..2133)
                     /locus_tag="VFY44_01335"
     CDS             complement(1711..2133)
                     /locus_tag="VFY44_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909102.1"
                     /translation="MSKGKNAKKKQPVAEPEAPGSVDTKRSVAVAGSTGTRRSVAVAG
                     SADTRGSAAVAGSAYTRASAGVVASAATAGSAAVAGSAATAAGAAILACVGTLFCVAC
                     SRCALCIGCVDCEDCAFCIGCVGLRGQSFKLGARRRRR"
     gene            2171..2440
                     /locus_tag="VFY44_01340"
     CDS             2171..2440
                     /locus_tag="VFY44_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909103.1"
                     /translation="MAKRDKRLYKSLRTAGVRKRAARTAAEAAERGGEGGHRVLAAIA
                     QELHEAGDLLDARTRAVEAGRSESERADPDRHLRDAARQTAAKNV"
     gene            2443..3429
                     /locus_tag="VFY44_01345"
     CDS             2443..3429
                     /locus_tag="VFY44_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5909104.1"
                     /translation="MAADYWFASSTEEFTPTEILAQHAAAEQAGFDGLGISDHWAPWF
                     PDGRGTQAWVTMAASGQGTSLPIGTGVTPVVHHYHPGVIAQAFMSMEELYPGRVFLGV
                     GSGESLNEVPLGLDWPEPTEMLQRFEQGLEAITRLWDGETVTMDAGWFQLKEAKLYTR
                     ARSKPRMYVSAFGPQAAAIAARYGDGLWTLGDPEQAPGIIDAYRTECGQTGKDVGQII
                     LQSGMAWAEDDDAAVQGARRWKPTQLPELYSDDIHDPDDMQRRADEQLTDREFADEGF
                     IVGADLDEHVRRVAEIEDLGATSVCLQLIGQADPMGTIARYGDTVLPALRER"
BASE COUNT          463 a         1329 c         1289 g          438 t
ORIGIN      
        1 agctgggcct gcgctcacgc cgcgagctgc ccggcgccct ggccgctgcc taggggggtt
       61 gcccccaaga caggggtggc gggttggggg tcccctcgat gcccggtgcc cgaatcgggc
      121 ggaaggtggc gtcatggaag agacgacagt cgacctccac ctccagctgc acgtcgaggg
      181 cgagcgcctt cacggccatc tggggcgcga ggaagacgaa ccgcgggagt tctcgggctg
      241 gctcggattc gtgtcggcac tcgacgcgct gatcgccgag gcgaccgccc ccaccaatgg
      301 ctcgcaaccc aggaggacga catgaccgtc gccgcacccc ccgccgacca cgtacgcgcg
      361 ctgcgctccc gcatgaccgg gaccgcgctg gccccgggcg acaccggcta cgacgaggcc
      421 cgcgtggcct tcaacctgat cgtgcagcag accccgccgc tgatcgcgtt ccccgcggac
      481 gccgacgacg tgggccaggt ggtgcgcttc gcccgtgagc gcgggttccg ggtcgcgccc
      541 cagcgcaccg gccactgcgc cgcgccgatc gagacgctgg accagaccat cctgctgcgc
      601 accgaccccc tgggcggcgt ccaggtggac gccgcggctc gccgtgcccg cgtcggcggc
      661 ggcgtgcagt ggcaggacgt ggtcccggtg gccgccgagt ccggcctgtc ggccctgcac
      721 ggctcggccg ccgacatcgg cgtcgccggc tactcgctgg gcggcggcat gggctggcag
      781 gcccgcaagc acggcctcca ggccaacagc gtgaccgcgg tcgagctcat caccgccggc
      841 ggcgagctcg tgcgcgccga ccgcgacaac gagcccgagc tgttctgggc cctgcgcggc
      901 ggcggcggca acttcggcgt ggtcaccgcg ctggagttcg acctctaccc gctggagacc
      961 gtgttcgccg ggatgatgtt cttcggcctg gagcaggcgc ccgaggtgtt ccacacctgg
     1021 cgtgagctgc tggccggcgt gcccgacgag ctgacctcga cctgccgcat cctgcgcttc
     1081 ccggacatcc cggagctgcc cgacgtggtg cgcggccaga acgtggtcgt ggtcaacggc
     1141 gcctacctgg gcgccgaggc cgagggccgg gacctgctgg ccccgctggc cgatctgggc
     1201 ccgggcatgg acacctgggg gatggtgccg gccgcggccc tgaccgagct gcacatggac
     1261 ccgccggacc ccgtcccggc ccagaccgac cacctgctgg tggacctgtc ggccgagggc
     1321 ttcgaccgca tgctggcggc gaccgggccc ggggtggaca cgccgctggg catggtcgag
     1381 ctgcgccaca cgggcggcgc cctgggccgc tcggagcccg ggcacggcgc gctggaccac
     1441 ctgccgggct cgtactcggc gttcgccggc ggcatggtcg tggacgccga gtccgccgcc
     1501 gcggtcggag gggacctgga tcgctggcgc gcgtcgctgg cgccctaccg cgcgggcgag
     1561 tacctgaact tcgtcgagcg gccgacggac gtggccgccg cctacggcga gggacggtac
     1621 gagcggctcg cgcgcgtcaa ggccgcgttc gaccccgacg aggtgttccg cgcgaaccac
     1681 ggcatccggc cgcgggcctg acggccgcgt ctacctgcgt cgcctgcgcg cgccgagctt
     1741 gaagctctgg ccgcgcaggc cgacgcagcc gatgcagaag gcgcagtcct cgcagtcgac
     1801 gcagccgatg cacagcgcgc accggctgca ggccacgcag aacagcgtgc ccacgcaggc
     1861 gaggatggcg gctccggcgg cggtggccgc gctgccggcc acggcggcgc tgcccgcggt
     1921 ggccgcgctg gccacgacgc cggcgctggc gcgggtgtag gcgctgcccg ccacggcggc
     1981 gctgccccgg gtgtcggcgc tgccggcgac ggccacgctg cgccgggtcc cggtgctgcc
     2041 ggccaccgcg acgctgcgct tggtgtcgac cgagccgggg gcctcgggct cggcgaccgg
     2101 ctgcttcttc ttggcgttct tgcccttgga cacggtgcgt gcacgctaca gcgccgggta
     2161 gtaccggcac atggccaagc gtgacaagcg cctctacaag tccctccgta ccgccggcgt
     2221 gcgcaagcgc gcggcccgca ccgccgccga ggcggccgag cgcggcggcg agggcggcca
     2281 ccgcgtgctg gccgccatcg cccaggagct gcacgaggcc ggcgacctgc tggacgcgcg
     2341 cacccgcgcg gtcgaggccg ggcgcagcga gtccgagcgc gccgatccgg accgccacct
     2401 gcgcgacgcc gcccgccaga ccgccgcgaa gaacgtctga ccatggccgc cgactactgg
     2461 ttcgcctcct ccaccgagga gttcaccccc accgagatcc tggcccagca cgccgccgcc
     2521 gagcaggccg gcttcgacgg cctgggcatc tccgaccact gggcgccgtg gttccccgac
     2581 ggccgcggga cccaggcctg ggtcaccatg gcggcctcgg gccagggcac ctcgctgccc
     2641 atcggcaccg gcgtcacgcc ggtggtgcac cactaccacc cgggtgtgat cgcgcaggcc
     2701 ttcatgtcga tggaggagct ctatcccggc cgcgtcttcc tgggcgtcgg ctccggtgag
     2761 tcgctgaacg aggtgccgct gggcctggac tggcccgagc cgaccgagat gctccagcgc
     2821 ttcgagcagg gcctggaggc catcacccgg ctgtgggacg gcgagaccgt gaccatggac
     2881 gccggctggt tccagctcaa ggaggccaag ctctacacgc gcgcgcggtc gaagccgcgc
     2941 atgtacgtgt cggccttcgg gccgcaggcc gcggccatcg ccgcgcgcta cggcgacggg
     3001 ctgtggacgc tcggggaccc cgagcaggcc cccgggatca tcgacgccta ccgcaccgag
     3061 tgcgggcaga ccggcaagga cgtgggccag atcatcctgc agtcgggcat ggcctgggcc
     3121 gaggacgacg acgccgcggt gcagggcgcc cggcgctgga agccgaccca gcttcccgag
     3181 ctctacagcg acgacatcca cgacccggac gacatgcagc gccgcgccga cgagcagctg
     3241 accgaccgcg agttcgccga cgagggcttc atcgtcggcg ccgacctgga cgagcacgtg
     3301 cgccgggtgg ccgagatcga ggacctgggc gccacctcgg tgtgcctcca gctgatcggc
     3361 caggccgacc cgatgggcac gatcgcccgg tacggcgaca cggtgctgcc ggcgctgcgc
     3421 gagcgctagc ccgagaaagc ggactcatgg gtccgcttcg ctgctagcct ggcctgcttg
     3481 ccgcagacga cccccacagc agccccccgc gcgaacccc
//
LOCUS       DASUPW010000057         3655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1674982, whole genome shotgun sequence.
ACCESSION   DASUPW010000057 DASUPW010000000
VERSION     DASUPW010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674982"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VFY44_01350"
     CDS             <1..811
                     /locus_tag="VFY44_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909105.1"
                     /translation="GVWQASVSFHQRLEALRMPHAWKDYGPGCHTVANFQRETLDTLA
                     VFERVYADPPAAPAKFEHRSIAPRFRVWGWDVAADPGRAVEFMRLDAAGDLRLALTGS
                     GTTTVTSPATFRGLRAVDVDTPTGRRVARPDRTGRIRFTVPLGAPHRAQQYTAAARAA
                     GQDRPGYFTRASVRLAPHARVLVAARRRKRALKVCLRGIGGAVPTARLRVRGKGGRAV
                     TRSLKVRVGEKRRCRTVARGRVKRGRYTVVVRARDGYGHALSERKVVRARR"
     gene            840..2621
                     /locus_tag="VFY44_01355"
     CDS             840..2621
                     /locus_tag="VFY44_01355"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX5909106.1"
                     /translation="MFSKVLVANRGEIAIRVMRTLEELGVASVAVYSEPDRDAPHVQR
                     ADEAYLLGGGPANESYLNVEKILEVAKESGAEAIHPGYGFLAENAPFVRACDEAGITF
                     IGPPASAIDAMGSKTKARELMKNAGVPIVPGTTDPVETVEDAKKLIDDEIGYPVAIKA
                     AGGGGGKGFRVALEESELKDAFEGAAREGEKFFSDATVYLERYLPDPRHVEVQVLADK
                     SGNTVHLFERDCSVQRRHQKLIEESPAPAVDPELREKIGKIGTDAAKAVGYFSAGTIE
                     GLLSDDGEYFFLEMNTRVQVEHCVTEMVTGIDIVREQIKIAAGEDLAFAQEDIQMRGH
                     AIECRINAETAATNFAPAPGQITHYVEPSGPGVRVDSGAREGYEVLPLYDPMIAKLIV
                     WDVDREASTRRMIRALSEYEIGGLTTLIKFHSTLMDTDQWRNGETCHDLVEDQEWLKA
                     LAEDPPAPADDEAEKVERDYSVEVSGRKFDVKVIGEAFAGGAVAGPANGAGAAPPKRG
                     ERKKSAGGGPDQITSPLQGNMWKVVVSEGDEVEEGQLLCIIEAMKMENEINAHKAGTI
                     AELSITEGEPIKTGDPIATITSPPAED"
     gene            complement(2646..3299)
                     /locus_tag="VFY44_01360"
     CDS             complement(2646..3299)
                     /locus_tag="VFY44_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909107.1"
                     /translation="MRLTLALGTAALAITPAAAEAQKPPKTPKGGKLTLAATPNPVTY
                     GGAVRVSGRLTGPSNGGQAVAVQSDLYPFGAFATVATAATTAAGDYAVAQRPLVNTRY
                     RAMKGAELSGVVQVSVRPRVSLRLGDYTPKRGQSVRFSGRVCPQHDGTALLLQRRTAT
                     GYRTVRRTTLRDIAGSTCSSYARAFRVYSDGRYRAAIARHGDHTTGVSASRVANAHR"
     gene            complement(3381..>3655)
                     /locus_tag="VFY44_01365"
     CDS             complement(3381..>3655)
                     /locus_tag="VFY44_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909108.1"
                     /translation="CRVSAGGRRGCRVRVLARQGRRLRTIAYTRVTVTGTSRVARLKL
                     NRRGRRLLARAPRRGVRVRIRLRASGPGYDVGRASKRFRLRAARPR"
BASE COUNT          522 a         1329 c         1347 g          457 t
ORIGIN      
        1 gggggtctgg caggccagcg tgtccttcca ccagcggctc gaggcgctgc gcatgccgca
       61 cgcgtggaag gactacggcc ccggctgcca cacggtcgcg aacttccagc gcgagaccct
      121 ggacacgctc gcggtgttcg agcgcgtgta cgccgacccg cccgcggccc cggcgaagtt
      181 cgagcaccgc tcgatcgcgc cccggttccg ggtctggggc tgggacgtcg cggccgaccc
      241 cggccgggcg gtggagttca tgcgcctcga cgcggcgggg gacctgcggc tggccctgac
      301 cgggtcgggc acgaccacgg tgacctcgcc ggccacgttc cgcgggctgc gcgcggtgga
      361 cgtggacacg cctaccggga ggcgcgtcgc ccgcccggac cgcaccgggc gcatccgctt
      421 cacggtcccg ctgggcgccc cgcaccgcgc gcagcagtac acggcggccg cgcgggccgc
      481 gggccaggac cggccgggct atttcacgcg cgcgtccgtc cgcctggcgc cccacgcgcg
      541 ggtgctggtg gccgcgcggc ggcgcaagcg ggcgctgaag gtgtgcctgc ggggcatcgg
      601 cggcgcggtg ccgacggccc ggctgcgcgt gcgcggcaag ggcggaaggg cggttacgcg
      661 ctcgctgaag gtcagggtgg gcgagaagcg gcgttgccgc accgtggccc gcgggcgggt
      721 caagcgcggc cgctacacgg tggtcgtgcg ggcgcgcgac ggctacggcc acgcgctcag
      781 cgagcgcaag gtcgtccgcg cccgccgctg accccgcccg acggatatcc tcccgcgccg
      841 tgttctccaa ggtcctagta gccaaccgcg gcgagatcgc catccgtgtc atgcgcaccc
      901 tcgaggagct gggtgtcgcg tcggtggccg tctactccga gcccgatcgc gatgcccccc
      961 acgtccagcg cgccgacgag gcgtacctgc tgggcggagg cccggccaac gagagctatc
     1021 tgaacgtgga gaagatcctg gaggtcgcca aggagtcggg ggccgaggcc atccacccgg
     1081 gctacggctt cctggccgag aacgcgccgt tcgtgcgcgc ctgcgacgag gccggcatca
     1141 cgttcatcgg cccgcccgcc agcgccatcg acgccatggg ctccaagacc aaggcccgcg
     1201 agctgatgaa gaacgccggg gtgccgatcg tgccgggcac gaccgacccg gtcgagaccg
     1261 tcgaggacgc caagaagctg atcgacgacg agatcggcta cccggtggcc atcaaggccg
     1321 cgggcggcgg tggcggcaag ggcttccgcg tggccctgga ggagtccgag ctcaaggacg
     1381 ccttcgaggg cgccgcccgt gagggcgaga agttcttctc cgacgccacg gtctacctgg
     1441 agcgctacct gcccgacccg cgccacgtcg aggtccaggt cctggccgac aagtccggca
     1501 acaccgtgca cctgttcgag cgcgactgct cggtccagcg ccgccaccag aagctgatcg
     1561 aggagagccc cgccccggcc gtcgaccccg agctgcgcga gaagatcggc aagatcggga
     1621 ccgacgccgc aaaggccgtc ggctacttct cggccggcac gatcgagggc ctgctgtccg
     1681 acgacggcga gtacttcttc ctcgagatga acacccgggt gcaggtcgag cactgcgtga
     1741 ccgagatggt caccggcatc gacatcgtgc gcgagcagat caagatcgcc gcgggcgagg
     1801 acctggcctt cgcccaggag gacatccaga tgcgcgggca cgccatcgag tgccgcatca
     1861 acgcggagac ggcggccacg aacttcgccc ccgcgcccgg ccagatcacc cactacgtgg
     1921 agccctcggg ccccggcgtg cgcgtcgact ccggcgcgcg cgagggctac gaggtgctgc
     1981 cgctgtacga cccgatgatc gccaagctca tcgtctggga cgtcgatcgc gaggcgtcga
     2041 cccgccgcat gatccgcgcc ctctccgagt acgagatcgg cggcctgacc acgctgatca
     2101 agttccactc cacgctgatg gacaccgacc agtggcgcaa cggcgagacc tgccacgacc
     2161 tggtcgagga ccaggagtgg ctcaaggcgc tggccgagga tccgcccgcc ccggccgacg
     2221 acgaggccga gaaggtcgag cgcgactact cggtcgaggt gtcgggccgc aagttcgacg
     2281 tcaaggtcat cggcgaggcg ttcgccggcg gcgcggtggc cggcccggcc aacggcgcgg
     2341 gcgcggcccc gcccaagcgc ggcgagcgca agaagtcggc cggtggcggc ccggaccaga
     2401 tcacctcgcc gctccagggc aacatgtgga aggtcgtggt ctccgaaggc gacgaggtcg
     2461 aagagggcca gctgctctgc atcatcgagg cgatgaagat ggagaacgag atcaacgccc
     2521 acaaggcggg cacgatcgcc gagctgtcca tcaccgaggg cgagccgatc aagaccggcg
     2581 acccgatcgc caccatcacc tccccgcccg ccgaggactg acccggcggg cggcctccgg
     2641 gccgctcagc ggtgggcgtt ggccacccgc gaggcgctga cccccgtcgt gtggtcgccg
     2701 tgcctggcga tcgccgcgcg gtagcggccg tcgctgtaga cccggaacgc gcgcgcgtag
     2761 ctcgagcacg tggacccggc gatgtcgcgc agggtcgtcc tgcggaccgt ccggtatccg
     2821 gtggccgtac ggcgctggag cagcagcgcg gtcccgtcgt gctggggaca gacgcgcccg
     2881 ctgaagcgca cgctctggcc gcgcttgggc gtgtagtcgc cgagccgcag cgacacccgc
     2941 gggcggacgg acacctggac cactccggag agctccgcgc ccttcatcgc ccggtagcgc
     3001 gtgttcacga gcggcctctg cgcgaccgcg taatcgcccg cggcggtggt ggcggccgtg
     3061 gcgacggtcg cgaacgcgcc gaacgggtag aggtcggact gcaccgccac cgcctggccg
     3121 ccgttcgacg ggccggtcag cctgccggac acccgcacgg cgccgccgta ggtgaccggg
     3181 ttcggcgtcg cggccagcgt cagcttgccg cccttcgggg tcttgggcgg cttctgggcc
     3241 tcggccgccg cgggcgtgat ggccagggcg gccgtgccca gggccagcgt gagtctcatg
     3301 gggttcatcg ttctcctcgc gtgatggtcg tcacgggttg gaacgcgccc cgagggcgaa
     3361 ggttcgccgg tgacggccgg tcagcgcggc cgggcggccc gcagccggaa ccgcttcgac
     3421 gcccggccga cgtcgtatcc gggcccgctg gcacgcagcc tgatccggac ccggacgccc
     3481 cggcgtggtg cccgtgccag cagccggcgc ccgcggcggt tcagcttcag ccgcgccacc
     3541 ctgctggtgc cggtcaccgt gacgcgcgtg taggcgatcg tccgcaggcg gcggccctgg
     3601 cgcgcgagca cgcggacgcg gcacccgcgc cgcccgccgg cgctgacccg gcagc
//
LOCUS       DASUPW010000058         2294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1536327, whole genome shotgun sequence.
ACCESSION   DASUPW010000058 DASUPW010000000
VERSION     DASUPW010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536327"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..812
                     /locus_tag="VFY44_01370"
     CDS             189..812
                     /locus_tag="VFY44_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909109.1"
                     /translation="MRRAVPTPATAIAFVALLVAFTGSSFAAPAREAASKLVSGSKIK
                     KESITGKQVKNGSLLAADFKSGQLPRGAAGAQGPAGARGATGAAGAQGPQGPAGPQGP
                     GAPVAYGVIDDDGTLRPTQSRGVVAARRANGGTSPGQFEVDFDRDVTRCAFSLTPVDD
                     LSSTTTGAISGMHPLNTQQPQNQIDVSTRNTSNTLAFTGPFAIVLYC"
     gene            complement(809..>2294)
                     /locus_tag="VFY44_01375"
     CDS             complement(809..>2294)
                     /locus_tag="VFY44_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909110.1"
                     /translation="SLRRDLRRCLPGAQPGGVGGGYRVGVADRSINPGADGTFAGRPV
                     HLGGYGFGSGPILTGRTADGILGPGVRVRAIALSDGKRAMAVADIETQGWFAATKDGP
                     YGLVDMRKRVQAATGGALTAGATVIQSDHSHSGPDTMGVWGGVPAAYRQMIADRTVEA
                     IVEAFRTMQPGTLYYGTAPGRDLLSNQFDYDAANQAVDSDVRVLQARTAAGRPFATLL
                     NFSAHPTVLGSGNRKVSGDWVSSTGPALARRFGGEAMTMVGTLGRTQPADRGCGAAPL
                     PPAGPARDLCSIDAYAERVAERAEDAAVGARPLGGNAMVEAQSFLVQDVATNAPILGL
                     NVAGGPAGAELNRAITPPWLTGTVLGTITGSARIGDVLLSTMPGEAYPQIPLKVRELV
                     QARGHMTAGLAGDQLGYLIAPYQAYPEPIRRTFFNQRGDEVSPLDNDNYFFNVSPTLG
                     ERVTCSLLRGAGDLFGRGTAVRDSQQGCAAFVNDALSPAGADVR"
BASE COUNT          313 a          864 c          829 g          288 t
ORIGIN      
        1 gccccgggtc gcgccgtcca gccgggcctc cagcgcggtg agctcgtggt cgcgtccgat
       61 cagcatcgcg gcgcatcgta agcacgcgaa aacgcgatct ccgacaacgc tagggacttc
      121 cctggtgtgg cccccggagg cccactccat cgtggcgacc ccccgccaac ccacccggag
      181 gtcgacccat gagacgtgca gttcccaccc ccgccaccgc catcgcgttc gtcgcgctgc
      241 tggtggcctt caccggctcc tcgttcgccg cgccggcccg cgaggccgct tccaagctgg
      301 tcagcggcag caagatcaag aaggagagca tcaccggcaa gcaggtcaag aacggctcgc
      361 tgctggcggc cgatttcaag tccgggcagc tgccgcgcgg cgccgccggc gcccagggcc
      421 ccgcgggcgc ccgcggtgcg accggagccg ccggcgccca ggggccgcag ggcccggccg
      481 gcccgcaggg acccggcgca ccggtggcct acggcgtgat cgacgacgac ggcacgctgc
      541 gcccgaccca gagccgggga gtggtggccg cgcgccgggc caacgggggc accagcccgg
      601 gccagttcga ggtcgacttc gaccgcgacg tgacccggtg cgcgttctcg ctgaccccgg
      661 tcgacgacct gagctcgacc accaccggcg ccatcagcgg catgcacccg ctgaacaccc
      721 agcagcctca gaaccagatc gacgtgtcga cccgcaacac gtcgaacacg ctggccttca
      781 cgggcccgtt cgcgatcgtc ctctactgct agcggacgtc ggctccagca gggctcagcg
      841 cgtcgttcac gaacgccgcg cagccctgct gggagtcgcg caccgcggtg ccgcggccga
      901 acaggtcgcc ggcgccgcgg agcagcgagc acgtgacgcg ctcgccaagg gtgggcgaga
      961 cgttgaagaa gtagttgtcg ttgtccagcg gcgacacctc gtcgccacgc tggttgaaga
     1021 acgtgcgccg gatcggctcg gggtaggcct ggtagggggc gatcaggtag cccagctggt
     1081 cgccggccag ccccgcggtc atgtggccgc gggcctgcac cagctcgcgc accttcagcg
     1141 ggatctgcgg gtaggcctcg cccggcatcg tggacagcag cacgtccccg atccgggcgc
     1201 tgcccgtgat cgtgcccagc acggtgccgg tcagccacgg cggcgtgatc gcgcggttga
     1261 gctccgcgcc ggcgggcccg ccggccacgt tcaggcccag gatcggagca ttggtggcca
     1321 cgtcctggac gaggaacgac tgcgcctcca ccatcgcgtt ccctcccagc ggcctggcgc
     1381 ccaccgccgc gtcctcggcg cgctcggcca cgcgctcggc gtaggcgtcg atcgagcaca
     1441 ggtcgcgcgc cgggccggcg ggcggcaacg gtgccgcgcc gcagccgcgg tctgcgggct
     1501 gggtgcggcc cagggtgccc accatggtca tggcctcgcc accgaagcgc cgggccaggg
     1561 cgggcccggt cgacgagacc cagtcccccg acaccttgcg gttgccgctg cccagcacgg
     1621 tggggtgggc cgagaagttc agcagcgtgg cgaacggccg gcccgcggcc gtgcgcgcct
     1681 ggagcacgcg aacgtcggag tccacggcct gattggcggc gtcgtagtcg aactggttcg
     1741 acagcaggtc gcgacccggc gccgtgccgt agtagagcgt gcccggctgc atggtgcgga
     1801 aggcctcgac gatggcctcg acggtgcggt cggcgatcat ctgccggtag gccgcgggca
     1861 cgccgcccca gacccccatc gtgtcggggc ccgagtgcga gtggtcggat tggatcacgg
     1921 tcgcgcccgc ggtcagggcg ccgcccgtgg cggcctggac ccgcttgcgc atgtccacca
     1981 gtccgtaggg gccgtccttg gtcgcggcga accagccctg ggtctcgatg tccgccacgg
     2041 ccatcgcacg cttgccgtcg gacagcgcga tcgcgcgcac gcgcacgccc gggcccagga
     2101 tgccgtccgc ggtgcgcccg gtcaggatcg gccccgagcc gaagccgtat ccgcccaggt
     2161 gcaccggccg gccggcgaac gttccgtcgg cgccggggtt gatcgagcgg tcggcgacgc
     2221 cgaccctgta gccgccaccc acgccgcccg gctgggcgcc gggcaggcac cggcgcaggt
     2281 cgcgccgcag ggac
//
LOCUS       DASUPW010000059         2009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_705346, whole genome shotgun sequence.
ACCESSION   DASUPW010000059 DASUPW010000000
VERSION     DASUPW010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705346"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VFY44_01380"
     CDS             complement(<1..500)
                     /locus_tag="VFY44_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909111.1"
                     /translation="MTSHPGIVRTVTWNLFHGRDHPPDPALFTLPSRLLRRTERNQTH
                     VQVNRPLRAEFVSVLDGVGWDVALLQEAPPRWLPALRQGCRSDGALALTSRNSLGWLR
                     AAAAALNPDLIASGEGGSNMILVRAPGAVQATETVELARAPERRTMLLARLRLPTGER
                     LCAACM"
     gene            489..1397
                     /gene="argF"
                     /locus_tag="VFY44_01385"
     CDS             489..1397
                     /gene="argF"
                     /locus_tag="VFY44_01385"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX5909112.1"
                     /translation="MTRHFLTGEELTAEELSALLDRAAQLKADRQASKALAGRSVALI
                     FEQPSTRTRVSFEVGVSELGGHPLVLREGEMQISRGESIRDTAQVLSRFVSAIGVRTV
                     PHADVAGLAEYATVPVINMLTGEHHPCQALADLLTLRERFGDLDGLKLAYVGDGNNVA
                     SSLMLLGAKAGLEVAVATPAELPPDPDVARAAAAAATGSGSVTLTGDPAEAVRGAAAV
                     YVDVWVSMGDRDADERRRLLDPYRLDGALLAQAGPGAIALHCLPAHPGEEITEEVLYG
                     AQSAVFDQAENRLHAQKALLELLAGA"
     gene            1407..1823
                     /locus_tag="VFY44_01390"
     CDS             1407..1823
                     /locus_tag="VFY44_01390"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX5909113.1"
                     /translation="MTRMLSILMACVVLVFVAGCGGDDSDSSDSGGGGGAAEKPAEPS
                     GGSKAPAGKADVTVDMKDIKFMPANVNVKKGGTVGWVNKDSVPHNVTKEDGPGPDFAS
                     ETVDGGGKYSQKFTVAGKVDYVCTIHPNQTGSVTVK"
     gene            complement(1816..>2009)
                     /locus_tag="VFY44_01395"
     CDS             complement(1816..>2009)
                     /locus_tag="VFY44_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909114.1"
                     /translation="GPEATRRARRDAVQRAMADARRRAEDKLDRSEPREQTELDEATR
                     EEARTEARRDAKEKAAAAI"
BASE COUNT          276 a          715 c          756 g          262 t
ORIGIN      
        1 tgcatgcagg ccgcgcacag ccgctcaccg gtgggcagcc gcaggcgcgc cagcagcatc
       61 gtccgccgct cgggggcccg ggccagctcc accgtctcgg tggcctggac cgcgcccggt
      121 gcgcggacca ggatcatgtt cgacccgccc tccccggacg cgatcaggtc cgggttcagc
      181 gcggccgccg cggcgcgcag ccacccgagc gagttgcgcg aggtcagcgc cagcgcgccg
      241 tccgagcggc acccctgccg cagcgccggc agccaccgcg gcggcgcctc ctgcagcagc
      301 gcgacgtccc agcccacgcc gtccagcacc gatacgaact ccgcccgcag cggccggttg
      361 acctggacgt gggtctggtt gcgctcggtc cgccggagca gccgcgaggg caaggtgaac
      421 agcgccggat cgggcgggtg gtcgcgcccg tggaacaggt tccaggtgac ggtacgtacg
      481 atgcccgggt gactcgtcat ttcctgaccg gggaggagct tacggccgag gagctgtccg
      541 ccctgctgga ccgggcggcc cagctgaagg ccgaccgcca ggcgtcgaag gcgctggcgg
      601 ggcgcagcgt ggcgctgatc ttcgagcaac cgtccacgcg cacgcgtgtc tcgttcgagg
      661 tcggggtgtc cgagctgggc ggccacccgc tggtgctgcg cgagggtgag atgcagatct
      721 cgcgcggcga gtcgatccgc gacacggcgc aggtgctgtc gcggttcgtg tccgcgatcg
      781 gcgtgcgcac ggtcccccac gccgacgtgg ccggcctggc cgagtacgcc accgtgccgg
      841 tgatcaacat gctcacgggc gagcaccacc cgtgccaggc gctggccgac ctgctgaccc
      901 tgcgggagcg cttcggcgac ctggacgggc tgaagctggc ctacgtgggc gacggcaaca
      961 acgtggccag ctcgctgatg ctgctgggag ccaaggccgg gctggaggtg gcggtggcca
     1021 cgccggccga gctgccgccc gatcccgacg tggcccgggc ggccgcagcc gcggccaccg
     1081 gatcgggctc ggtgaccctg accggggatc cggccgaggc cgtgcgcggc gccgcggccg
     1141 tctacgtgga cgtgtgggtg agcatgggcg accgggacgc ggacgagcgt cgccggctgc
     1201 tcgaccccta ccgcctggac ggcgcgttgc tggcccaggc cggccccggc gcgatcgcgc
     1261 tgcactgcct gcccgcgcac cccggcgagg agatcaccga ggaagtgttg tacggcgccc
     1321 agtccgcggt cttcgaccag gctgagaacc ggctgcatgc gcagaaggcg ttgctcgaac
     1381 tgctcgcagg agcgtaggct ggacccatga cccgcatgct gagcatcctg atggcgtgcg
     1441 tcgtgctggt gttcgtggcc ggctgcggcg gagacgactc ggactcgtcg gacagcggcg
     1501 gcggaggcgg cgcggccgag aagccggcgg agccctcggg cgggagcaag gcgccggcgg
     1561 gcaaggccga cgtcacggtg gacatgaagg acatcaagtt catgcccgcc aacgtgaacg
     1621 tgaagaaggg cggcaccgtg ggctgggtca acaaggactc cgtgccccac aacgtgacca
     1681 aggaggacgg ccccgggccc gacttcgcct cggagaccgt ggacggcggc ggcaagtaca
     1741 gccagaagtt caccgtggcc ggcaaggtcg actacgtctg cacgatccac ccgaaccaga
     1801 ccggttcggt aaccgtcaaa tagcggcggc ggccttctcc ttcgcgtcgc ggcgcgcctc
     1861 cgtgcgcgcc tcctcgcggg tggcctcgtc cagctcggtc tgctcgcgcg gctcggagcg
     1921 gtccagcttg tcctccgccc gccggcgcgc gtcggccatc gcgcgctgca cggcgtcgcg
     1981 gcgcgcgcgc cgggtggcct cgggtccca
//
LOCUS       DASUPW010000060         2550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1259132, whole genome shotgun sequence.
ACCESSION   DASUPW010000060 DASUPW010000000
VERSION     DASUPW010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259132"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VFY44_01400"
     CDS             <1..787
                     /locus_tag="VFY44_01400"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEX5909115.1"
                     /translation="EGQDMSGTAREQLAEVAQRMAEEYMQGDFPENWDIPELYTQLEQ
                     MYPVGIDLEEMDPNNADRAGVIAELREDIVEAYADREEELGPDIMRALERFMLLQIID
                     ERWREHLHDMDYLREGIHLRGFAQIEPLVAYKNEGFAMFTQLMNSIWEEFARYSFVVE
                     IEVDPEDNQPAQGTGSRWGGSTNSSSTASFSYAGGTSEEQPSALAAARQGQPVAEGEE
                     YYEDEPVQFVETRTVAEEDRVGRNDPCPCGSGKKYKKCHGAQR"
     gene            832..1863
                     /gene="prfB"
                     /locus_tag="VFY44_01405"
                     /pseudo
     CDS             832..1863
                     /gene="prfB"
                     /locus_tag="VFY44_01405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            complement(1860..2276)
                     /locus_tag="VFY44_01410"
     CDS             complement(1860..2276)
                     /locus_tag="VFY44_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909116.1"
                     /translation="MPSLKKLLLPITITALAAGLLLPSAASAQRICQVNPNHPICRQV
                     EKRQEIRDRQNGVTPACRAAADRYAEQNRRASILKGGIPAWKNAVKKAKTKKAKKKAK
                     KGLKKARKQHKKVAASLPGLKTSYDSACAGARVKPF"
BASE COUNT          405 a          859 c          930 g          356 t
ORIGIN      
        1 cgagggccag gacatgtcgg gcaccgcgcg cgagcagctg gccgaggtgg cccagcgcat
       61 ggccgaggag tacatgcagg gcgacttccc cgagaactgg gacatccccg agctgtacac
      121 ccagctggag cagatgtacc cggtgggcat cgacctcgag gagatggacc ccaacaacgc
      181 ggaccgcgcc ggggtcatcg ccgagctgcg cgaggacatc gtcgaggcct acgccgaccg
      241 cgaggaggag ctggggccgg acatcatgcg cgccctggag cgcttcatgc tgctccagat
      301 catcgacgag cgctggcgcg agcacctgca cgacatggac tacctgcgcg agggcatcca
      361 cctgcgcggg ttcgcccaga tcgagccgct ggtggcgtac aagaacgagg gcttcgcgat
      421 gttcacccag ctgatgaact ccatctggga ggagttcgcg cgctacagct tcgtggtcga
      481 gatcgaggtg gacccggagg acaaccagcc ggcgcagggc accggctcgc gctggggcgg
      541 gtccaccaac tccagctcca ccgcctcctt cagctacgcc ggcggcacct ccgaggagca
      601 gcccagcgcg ctggccgccg cccgccaggg ccagcccgtg gccgagggcg aggagtacta
      661 cgaggacgag ccggtccagt tcgtcgagac ccgcaccgtg gccgaggagg acagggtcgg
      721 acgcaacgac ccgtgcccct gcggctcggg caagaaatac aagaagtgcc atggcgcaca
      781 gcgctagtgg cctccgcgac cagctgaggc tgctcgcgga ctacctttga cccgccctca
      841 cttgaggcac gcgccgccga gctagagcgc gagatggggg agccggggtt ctgggacgac
      901 caggaccggg ccgccgaggt gtcggccgag cacgcgcgca cgcagcgcaa ggtcgacgag
      961 ttcaacacgc tggaggccga cctcggcgac ctggagacca tggaggagat ggcggccgag
     1021 gacgcctcga tcgccgccga ggtcgacgag cagagcgcct cgatcgcgtc gcggctggag
     1081 acgctcgagg aggcgcgcct gttcgccggc gagtacgacc acggcgatgc ggtggtcacc
     1141 gtcaacgccg gggccggcgg caccgattcc caggactggg ccgagatgct cctgcgccag
     1201 gagatgcgct gggccgagag ccgcggcatg aaggtcgagc tgctggaggc ctcggccggg
     1261 gaggaggcgg gcatcaagtc ggccacgttc ctggccaagg gcgagaacgc ctacggcctg
     1321 ttcagcgccg agaagggcgt ccaccggctg gtgcgcatat cgccgttcga ctccgcggcg
     1381 cggcgccaca ccgcgttcgc cgggctcgag gtcgcgccgt tcctggagtc atcgggcgag
     1441 atcgagatcg agcccgacga ccttcagatc gacacctacc gcgcgtcggg ggccggcggc
     1501 cagcacgtga acaagaccga ctcggcggtg cggatcacgc acaagcccac gggcaccgtc
     1561 gttcagtgcc agaacgagcg ctcgcagggc gccaacaagc agacggccat ggccatgctg
     1621 acctccaagc tcgtggagct ggaggaggag cggcgccggg ccgagatcgc caaggagaag
     1681 ggtgaggccc aggacgccgc ctggggctca cagatccgct cgtacgtgct gcacccctac
     1741 acgatggtca aggaccaccg caccgacacc gaggtcggca acgtgcaggg cgtgctcgac
     1801 ggagggctcg acccgttcgt gcgggccgag ctcctccggc gggcaggtac cgccgccccc
     1861 tagaagggct tcacgcgtgc gccggcgcag gcgctgtcgt agctcgtctt caggccggga
     1921 agggacgccg cgaccttctt gtgctgcttc ctcgccttct tgagcccctt cttggccttc
     1981 ttcttggcct tcttggtctt ggccttcttg accgcgttct tccaggccgg gataccgccc
     2041 ttgaggatgg acgccctgcg gttctgctcg gcgtacctgt cggcagccgc gcggcaggcg
     2101 ggggtgacgc cgttctggcg gtcgcggatc tcctggcgct tctcgacctg gcggcagatc
     2161 gggtggttgg ggttcacctg gcagatgcgc tgggcgcttg ccgccgacgg cagcagcagg
     2221 ccggcggcca gcgccgtgat ggtgatcggg agcaggagct tcttgagcga tggcatgcgg
     2281 cgaacgtagg tcaggtgcgc tctgcccgcc actagcaccc ccgctagctt ttgcccgccg
     2341 gaggggggtc gggtacggtg gctttcgcgt gggtgcggca gacctttccg agctcggtgc
     2401 aggggccggc caaatggccc gagccatcgc cgtgatcggg cacgaagccc ctctgtggct
     2461 ggtcgcgggc gccgccgggc tggaccgcgc cgaggcggcc cgcgccgccg acgccctggt
     2521 cgcggccggc cggctggtgg ccgccgaccc
//
LOCUS       DASUPW010000061         5213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1675047, whole genome shotgun sequence.
ACCESSION   DASUPW010000061 DASUPW010000000
VERSION     DASUPW010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1675047"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..535)
                     /locus_tag="VFY44_01415"
     CDS             complement(179..535)
                     /locus_tag="VFY44_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5909117.1"
                     /translation="MEVVKRMWIDFREQGVEVALAHLHPDVEFQLDDGTVFSGHDGVR
                     DFFARFAGERRFAAAPYTFEPCGQGVIVAGHRRIQTPGGSESEYTYFSHHVRDGVITR
                     IAAWTDRDQATADLGG"
     gene            617..1777
                     /locus_tag="VFY44_01420"
     CDS             617..1777
                     /locus_tag="VFY44_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX5909118.1"
                     /translation="MDELAEVLRARLIEGARAGRAPGADVAARVRELVAAEAPALAEA
                     ERRALCRRVLRLAGGLGPLEPLLADPLVDEVMVNGPERVYVERRGRVEPTAVRFASEA
                     DLLHAIERILAPLGRRVDEASPLCDARLQDGSRVNVVVPPLSLEGPCLTIRRFRRQGF
                     SLRDLVTGGTLPAAAAELLALAVAGRASILVSGGTGSGKTTTLNALSGAIPAGQRIVT
                     IEDAAELALRQEHVVRLEARPPNLEGRGEVTIRRLVVNALRMRPDRIVVGEVRGPEAL
                     DLLQALNTGHDGSLSTVHANSPEDALRRLETLALMAGVGLPHQAVRDQVAGAVDLVVH
                     QERRADGSRGVVSLCQVVRAAGGAGTRELLHGDRVERPGDGPLARRLERVAA"
     gene            complement(2097..2558)
                     /locus_tag="VFY44_01425"
     CDS             complement(2097..2558)
                     /locus_tag="VFY44_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909119.1"
                     /translation="MSSDDAMPATNPGLASSASSAPPAGSSTTISPVNRAWAVAARTS
                     SSRRDWSPNASAHSRSSSARSPPARRWTRHAATSASIRDEATRARMDSKASSVRAPAA
                     SSPATRPSSFAAGPVSPAAASPRPARSECPPPSASASASATVAASASTARR"
     gene            2652..3473
                     /locus_tag="VFY44_01430"
     CDS             2652..3473
                     /locus_tag="VFY44_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX5909120.1"
                     /translation="MAFACAALAVAGLGALWPARATRAARVPRTMRALSGLGGRAAAR
                     APADLEGRIAAAGRPGGLGPREIMAAKVAGAVAGAPFGVLAGALAPGRLGLLVVVLTP
                     AAGFLGPDLWLRRRADDRARRVRRELPALLDLLRVSVAAGSSLAAAMADVGERATGPL
                     AAEWRAVGGEVALGVPLERALAGMSVRLPQAEVTALCAALDRARRHGAPLGDTLAGQA
                     RDARLALRRRVAEDAARAGPKIQLVVALLLVPSVLLLVAAALAAALLDSGGVSGI"
     gene            3812..4246
                     /gene="mraZ"
                     /locus_tag="VFY44_01435"
     CDS             3812..4246
                     /gene="mraZ"
                     /locus_tag="VFY44_01435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX5909121.1"
                     /translation="MAFRGHYEHSLDAKHRLSIPSKFREAFSDGLVLSPDTDACITVS
                     SPAAHEARVAQALEGKNPLGSQYKRIQRYFQGKSFEVELDSAGRVNIPASLLAHAGIQ
                     KEVVVTGVHDHLELWSSEGWTEQQAMLDQVMGEVTDSLGDPS"
     gene            4248..5207
                     /gene="rsmH"
                     /locus_tag="VFY44_01440"
     CDS             4248..5207
                     /gene="rsmH"
                     /locus_tag="VFY44_01440"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX5909122.1"
                     /translation="MTRTHIPVLAGELIDLTGPREGDRVVDCTFGAGGHARLVAERIG
                     ASGTLVCVDRDPDAEERFEELAAEVACHTRFLRMDYTEALALLEQEGFTADIVYFDLG
                     VSSMQIDTRERGFSYSYDAPLDMRMDPGQELDARTVVNTMEERQLAKVLSTYGADRNA
                     RVIAREIVRRRQKAPLETTADLVAAVEAALPAAVRRRFGGSSPAKRVFQAIRIVVNDE
                     LEDLERALPEAWSILDRDGGRLAAISFHSLEDRRVKHFLADRARGCICPPDLPICRCG
                     RTPEAELITRRSVAPTPGEVADNPRSGSARLRAARRIGDGVER"
BASE COUNT          565 a         1852 c         2119 g          677 t
ORIGIN      
        1 cagcccgcgg gcagcgaggc cagcagcgtg cccgccgggt aggccgcgct gaggagcccg
       61 gccgaggtct tcgacagccc caggtcgtcc tggtaggtgg gcagcagcgg cgtgatggcg
      121 gcgaagaaca tcgtgtccac cagcacggcg gcgcacacga acgggaagac cctccgcatc
      181 agccgcccag gtccgcggtg gcctggtccc ggtcggtcca ggccgcgatc cgcgtgatca
      241 ccccgtcacg cacgtggtgg gagaagtacg tgtactcgga ctcgctcccg ccgggggtct
      301 ggatccggcg gtggcccgcc acgatcacgc cctggccgca gggctcgaac gtgtagggcg
      361 cggcggcgaa ccggcgctcg cccgcaaagc gcgcgaagaa gtcgcgcacg ccgtcgtggc
      421 cgctgaacac ggtgccgtcg tccagctgga actcgacgtc cgggtgcagg tgtgccagcg
      481 ccacttcgac cccctgctcg cggaagtcga tccacatgcg cttgaccact tccatgcggg
      541 gatcgtccac gtgctgatgg tcgcacgtcg gggggtgagg tcgccccggc ccggcccttg
      601 accaggcatg ggctccatgg acgagcttgc cgaggtcctg cgggcgcgcc tgatcgaggg
      661 cgcgcgggcg ggccgggcgc cgggcgcgga cgtcgccgcg cgcgtgcgcg agctggtcgc
      721 ggccgaggcc cccgcgctgg cggaggccga gcgccgggcg ctgtgccggc gcgtcctgcg
      781 gctggcgggc gggctcggcc cgctcgagcc gctgctggcc gatccgctgg tggacgaggt
      841 catggtcaac ggcccggagc gggtctacgt cgagcggcgc ggccgggtgg agcccaccgc
      901 cgtccggttc gcctccgaag ccgacctgct gcacgcgatc gagcgcatcc tggcaccgct
      961 gggcaggcgc gtggacgagg cctcgccgtt gtgcgacgcg cggctccagg acggctcgcg
     1021 cgtgaacgtg gtcgtgccgc cgctgtcgct ggagggcccg tgcctgacca tccgccgctt
     1081 ccggcgccag ggcttctcgc tgcgcgacct ggtgaccgga ggcacgctgc ccgccgcggc
     1141 ggcagagctg ctggcgctgg cggtggccgg gcgggcgtcg atcctggtca gcggggggac
     1201 ggggtcgggc aagaccacca cgctcaacgc gctgtcgggc gccataccgg cggggcagcg
     1261 gatcgtgacc atcgaggacg cggcggagct ggcgctgcgc caggagcacg tggtcaggct
     1321 ggaggcgcgg ccgcccaacc tggagggcag gggagaggtg accatccggc ggctggtggt
     1381 caacgccctg cggatgcgcc ccgaccggat cgtggtcggc gaggtgcgcg ggcctgaggc
     1441 cctggacctg ctccaggcgc tgaacaccgg gcacgacggg tcgctgagca cggtgcacgc
     1501 caactcgccc gaggacgcgc tgcgccggct cgagacgctg gccctgatgg cgggggtggg
     1561 cctgccccac caggcggtgc gcgatcaggt ggcgggcgcg gtggacctgg tggtgcacca
     1621 ggagcgccgc gcggacggct cccggggggt cgtgtcgctg tgccaggtgg tgcgggcggc
     1681 gggcggcgcc ggcacgcgcg agctgctgca cggcgaccgg gtcgagcgcc cgggtgacgg
     1741 cccgctggcc cggcggctgg agcgggtggc cgcgtgagcg cggcgggctg gctggccttc
     1801 ggcgcggggg cggcgggcgc gctggcggcc gccgacctgg cgggcctcgt gcgcccggcg
     1861 gtggggcgcc gggcaccccg ggcggcgcgc gcgctcgggt cgctgctggg cgccgtggtg
     1921 gcggcggggc gcgaagggcg cgatcccgcc gcacgggaac gccggcggct gctggtcgcc
     1981 gggggcgcgc tcgcgttcgc cttcgggctg ctggcggggg gaccgtgggc ggcggccgtc
     2041 ctgggcgcgg gcggcccgtg ggtggtgggc cggctgctgg ccgcgcgccg ggcgcgctac
     2101 cggcgggcgg tggacgccga ggccgcgacc gtggcactgg cgctggccga cgcgctcggc
     2161 ggcgggcact cgctgcgggc gggcctgggg gaggcggccg ccgggctgac cggcccggcc
     2221 gcgaacgagc tgggccgggt cgcgggcgag ctggcggcgg gggcgcggac cgaggacgcc
     2281 ttggagtcca tgcgcgcgcg ggtggcctcg tcgcggatgg acgcgctggt ggcggcgtgc
     2341 ctggtccagc gccgggcggg cggcgacctg gcgctgctgc tgcgcgagtg cgccgaggcg
     2401 ttcggcgacc agtcgcgcct ggaggacgag gtgcgggccg ccaccgccca ggcccggttc
     2461 accgggctga tcgtggtgct gctgccggcc ggtggcgcgc tgctggccga gctggccagc
     2521 cccgggttcg tggcgggcat ggcgtcgtcg ctgctcaccg cgtggctcgt gggcgtcgcg
     2581 ctggtcctgc aggtcgtggc cgcggtggcc atccggcgcc tgggccgggt gcgctgggga
     2641 cggcggcggc gatggcgttc gcgtgcgccg cgctcgccgt ggccgggctg ggcgcgctgt
     2701 ggccggcgcg ggccacgcgg gccgcccgcg tgcccaggac gatgcgggcg ctctcggggc
     2761 tgggcgggcg cgcggccgct cgtgccccgg ccgacctgga agggcgcatc gccgccgcgg
     2821 ggcgcccggg cggcctcggg ccgcgcgaga tcatggcggc caaggtcgcg ggcgcggtgg
     2881 ccggagcgcc cttcggcgtc ctggccgggg cgctggcgcc gggccggctg gggctgctgg
     2941 tggtggtgct cacgcccgcg gccggcttcc tgggcccgga cctgtggctg cgccggcgcg
     3001 cggacgaccg ggcgcggcgc gtgcgccgcg agctaccggc gctgctggac ctgctgcgcg
     3061 tctcggtggc ggccggctcg tcgctggccg cggccatggc ggacgtgggg gagcgggcca
     3121 cggggccgct ggccgccgag tggcgcgccg taggcgggga ggtggcgctg ggcgtgccgc
     3181 tggagcgcgc cctggccggg atgtccgtcc ggctgcccca ggccgaggtc accgcgctgt
     3241 gcgccgcgct cgaccgcgcc cgccgccacg gcgccccgct gggggacacg ctggcgggtc
     3301 aggcccgcga cgcccggctg gcgcttcgcc gccgggtggc cgaggacgcc gcgcgggcgg
     3361 gccccaagat ccagctggtc gtggcgctgc tcctggtgcc ctcggtgctg ttgctcgtgg
     3421 ccgctgcgct ggcggcggcg ctgctggact ccggcggcgt aagcgggatc tgaccggcgc
     3481 cccacgatgt cccacccgga cgcaggattc gttgcggacc ggggcccgtc cgtgcaatcc
     3541 ggcgcccagc gcgcggcctt gcagcgcgcc gcccgcccgc gcgggcgtgc ccgcccacct
     3601 cctcccacgt cgcgtatccc gctctgtaga gcgccgtgcg gccccccggg cgtccaacat
     3661 ccggccagcg cggggagatt ggtggggtga tgtgttgcag ggtgggaggt agtggggtag
     3721 tgtggcgcca gctcacgagg accggagtgg cggggctcct cccactccgc cacttcggtc
     3781 cgagtcagcc ccccgcggaa gacccagctc catggcattc agaggccact acgaacattc
     3841 gcttgacgcg aagcaccgct tgagcattcc ctcaaagttc cgcgaagcct tctccgacgg
     3901 cctggtgctg tcgcccgaca ccgacgcgtg catcacggtg tcctcgcccg cggcgcacga
     3961 ggcgcgcgtg gcccaggcgc tcgagggcaa gaacccgctg ggctcccagt acaagcgcat
     4021 ccagcgctac ttccagggca agtcgttcga ggtcgagctg gactccgccg gccgcgtgaa
     4081 catcccggcc tccctcctcg cgcacgcggg gatccagaag gaggtcgtcg tcaccggggt
     4141 gcacgaccac ctggagctgt ggtcctcgga gggttggacc gagcagcagg ccatgcttga
     4201 ccaggtcatg ggggaggtga cggactccct tggcgatcct tcttgacatg acgcgcacgc
     4261 acataccggt tctcgccggc gagctgatcg acctgacggg gccccgcgag ggcgaccgcg
     4321 tggtggactg cacgttcggc gccggcggcc acgcccggct cgtggccgag cggatcggcg
     4381 cgtccgggac gctggtgtgc gtcgaccgcg accccgacgc cgaggagcgc ttcgaggagc
     4441 tggccgccga ggtcgcgtgc cacacgcgct tcctgcggat ggactacacc gaggccctgg
     4501 ccctgctcga gcaggagggc ttcaccgccg acatcgtgta cttcgacctc ggcgtctcgt
     4561 cgatgcagat cgacacgcgc gagcgcgggt tctcctactc ctacgacgcc ccgctggaca
     4621 tgcggatgga cccgggccag gagctggacg cgcgcacggt ggtcaacacc atggaggagc
     4681 gccagctggc caaggtcctc agcacctacg gcgcggatcg caacgcgcgc gtcatcgcac
     4741 gcgagatcgt ccgccgccgc cagaaggcgc cgctggagac cacggccgac ctcgtggcgg
     4801 ccgtcgaggc agccctcccg gccgccgtcc ggcgccgatt tggaggaagt tcccctgcaa
     4861 agcgcgtatt tcaagccatc cgcatcgtgg tcaacgacga gctggaggac ctggagcggg
     4921 cgctgcccga ggcctggtcg attctggacc gtgacggcgg gcgcctggcc gcgatctcgt
     4981 tccactcgct cgaggaccgg cgcgtcaagc acttcctggc cgaccgcgcg cggggatgca
     5041 tctgcccgcc cgacctgccg atctgccgct gcggccgcac gccggaggcc gagctgatca
     5101 cgcgccgctc ggtggcgccc acgcccggcg aggtggccga caacccgcgc tcgggctcgg
     5161 cgcgcctgcg cgcggcccgc cggatcggcg acggggtgga acgctgatgt ccg
//
LOCUS       DASUPW010000062         2174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_982504, whole genome shotgun sequence.
ACCESSION   DASUPW010000062 DASUPW010000000
VERSION     DASUPW010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982504"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VFY44_01445"
     CDS             complement(<1..241)
                     /locus_tag="VFY44_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909123.1"
                     /translation="MSRSSDRGSLALLLHSHMPYVEGFGTWPFGEEWLWEATAAVYLP
                     LIDLLNEAGAPLTLGITPVLADQLETLPGEAGERLV"
     gene            complement(238..993)
                     /locus_tag="VFY44_01450"
     CDS             complement(238..993)
                     /locus_tag="VFY44_01450"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5909124.1"
                     /translation="MSAEREYVQADQPDGVPPLALTGERTLPDVPEENYWFRRHLAVY
                     EWIAEQVAGQTVVDMACGEGYGTEVLARRAARVTGVDANPEAHAHAAAKYTAPGVRFE
                     RDLIDHYSEPCDAVVFLQTIEHVQDPAAVLRHFKAMAGVAFVSTPNLLTLAPEGAEKS
                     DNPWHVKEYRVEEFRELCESVFGRVELHGLFHARKLRLHELALSAGWDDVHAKLGVTK
                     PFYDRFTPAIAASDFTLRTDGLERALDFVAVLR"
     gene            1050..1967
                     /locus_tag="VFY44_01455"
     CDS             1050..1967
                     /locus_tag="VFY44_01455"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5909125.1"
                     /translation="MATAEVPTDYIRDANVRYHDMAAEHYDAKWGIDYGEPGQAQVTG
                     KLRKALGLKHLGHHPAALEIGAGTGYFGLNLVRAGVVREYTATDISPGMIAALEASAA
                     RLGVPVRTACCEAAELPFPDDSFDLVFGHAVLHHLPDLEGSFREFLRVLRPGGAIAFC
                     GEPSHYGDRIAALPKRAAYRAAPAWRALMRAAPATHEGFHRTADEDELERIVDVHAFT
                     PAMLRDSAGEAGFGQVLVKGEELAASMFGWANRALEATAEPSDVPWLWRQYAYRGYLA
                     FQALDRTLLEPRLPPALFYNLLISGRAPA"
BASE COUNT          283 a          831 c          773 g          287 t
ORIGIN      
        1 cgaccagccg ctcgccggcc tcgccgggaa gcgtctcgag ctggtcggcc agcacgggcg
       61 tgatgcccag cgtcagcggc gcccccgcct cgttcagcag gtcgatcagc ggcaggtaga
      121 cggcggccgt ggcctcccac agccactcct cgccgaacgg ccaggtgccg aagccctcga
      181 cgtagggcat gtggctgtgc agcagcagcg cgaggctgcc gcggtcagag ctccggctca
      241 tctgagcacg gccacgaagt ccagcgcgcg ctccaggcca tccgtgcgca gcgtgaagtc
      301 cgacgcggcg atggccgggg tgaagcggtc gtagaacggc ttggtcaccc ccagcttcgc
      361 gtgcacgtcg tcccagccgg cggacagggc cagctcgtgc agtcgcagct tgcgcgcgtg
      421 gaacaggccg tgcagctcca cgcggccgaa cacggactcg cacagctcgc ggaactcctc
      481 gacccggtac tccttgacgt gccacgggtt gtcggacttc tcggcgccct ccggcgccag
      541 cgtcagcagg ttgggggtcg agacgaaggc gacgccggcc atcgccttga agtggcgcag
      601 cacggccgcc ggatcctgca cgtgctcgat cgtctgtagg aacaccacgg cgtcgcaggg
      661 ctcggagtag tggtcgatca ggtcgcgctc gaaccgcacc cccggggcgg tgtatttggc
      721 ggcggcgtgc gcgtgagcct ccgggttggc gtccactccc gtcacgcgcg cggctcggcg
      781 ggccaggacc tcggtgccgt agccctcgcc gcaggccatg tccaccacgg tctgccccgc
      841 cacctgctcg gcgatccact cgtacacggc caggtgacgc cggaaccagt agttctcctc
      901 gggcacgtcg ggcagggtgc gctcgcccgt cagcgccagc gggggcacgc cgtcgggctg
      961 atcggcctgc acgtattcac gttcggctga cacgcggggc cagtgtatgg aggcggcctg
     1021 ggccgacccc gtctaccctg tcccggccca tggccaccgc cgaggtcccg accgactaca
     1081 tccgcgacgc caacgtgcgc taccacgaca tggcggcgga gcactacgac gccaagtggg
     1141 gcatcgacta cggcgagccc ggccaggccc aggtgaccgg caagctgcgc aaggcgctgg
     1201 ggctcaagca cctgggccac cacccggcgg cgctggagat cggcgccggc accggctact
     1261 tcggcctgaa cctggtgcgc gccggcgtgg tccgcgagta cacggccacc gacatctcgc
     1321 cggggatgat cgccgcgctg gaggccagcg ccgcgcggct cggggtgccg gtgcgcaccg
     1381 cctgctgcga ggccgccgag ctgcccttcc ccgacgactc cttcgacctg gtcttcggcc
     1441 acgcggtgct gcaccacctg cccgacctgg agggctcgtt ccgcgagttc ctgcgcgtgc
     1501 tgcgccccgg cggcgccatc gccttctgcg gcgagccgtc gcactacggc gaccggatcg
     1561 ccgccctgcc caagcgcgcg gcctaccgtg ccgcgccggc gtggcgggcg ctgatgcgcg
     1621 ccgcgcccgc cacccacgag ggcttccacc gcaccgccga cgaggacgag ctggagcgga
     1681 tcgtcgacgt gcacgccttc accccggcca tgctgcgcga cagcgcgggc gaggccggct
     1741 tcggccaggt gctggtcaag ggcgaggagc tggccgccag catgttcggc tgggccaacc
     1801 gcgcgctgga ggccaccgcc gagccctccg acgtgccgtg gctgtggcgc cagtacgcct
     1861 accgcggcta cctcgccttc caggcgctgg accggacgct gctggagccg cgcctgccgc
     1921 cggcgctgtt ctacaacctg ctgatctcgg gccgggcgcc ggcctaggcc ggcgttctcc
     1981 cgccggccgc ggcgtgcacg ccggggcccg cgagccgccc ggcagcgcgt gggtgggctc
     2041 gtgcgtgctc acgtgccgct ccccgcccac gagcagcgcc gtcgctcgcg ggcggcctgg
     2101 tgcacgcggc gggccgggcg cggcctaggc gaagaccgcc gccgcaggca ccagcgccgc
     2161 caccaccacc gccg
//
LOCUS       DASUPW010000063         2912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_290737, whole genome shotgun sequence.
ACCESSION   DASUPW010000063 DASUPW010000000
VERSION     DASUPW010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290737"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VFY44_01460"
     CDS             complement(<1..522)
                     /locus_tag="VFY44_01460"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5909126.1"
                     /translation="MVDFTLTDEQKDIQAMAHDFAENEIRPKAWEYDKEGTWPAEIFE
                     KAWELGLMNNHAPAEYGGPGLDYLTGAIIEEEISWGCSGIGTSLGANGLATAPLALGG
                     SEELQKKYFGMLTEAPLYASFCLTEPDAGSDVSGMKTTAVKKGDKYVINGSKCFITNG
                     SYASFYTVYAKTDK"
     gene            complement(660..797)
                     /locus_tag="VFY44_01465"
     CDS             complement(660..797)
                     /locus_tag="VFY44_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909127.1"
                     /translation="MLFERLRRRTMAPSRPLLYPEQVGLALFSLLTIGGGIYMVFWVL
                     F"
     gene            815..1234
                     /locus_tag="VFY44_01470"
     CDS             815..1234
                     /locus_tag="VFY44_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909128.1"
                     /translation="MEFGTVLIAVVLVAIVVACLSYIGSGAIYKGLGRTGLSLDEPDL
                     RPEPVAGSPAANAEAQEEIRQMLEAKSARSERRGDGPIDVDAEMAALSAPPVAADPAL
                     REEIRQLVVARNERRVRRGEDPLDVDAEVDRELQRLI"
     gene            1244..1588
                     /locus_tag="VFY44_01475"
     CDS             1244..1588
                     /locus_tag="VFY44_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909129.1"
                     /translation="MADDRKFEIDELLVRPGTYFNPQTEVLVVVDDSTSLESEIFNME
                     EFEGADWVLIADELPVDEAKRDELLEDFQTNVHGDVAVRATTVEDPAGEADDPDVDDD
                     PPRAVVPVDEEE"
     gene            1622..2677
                     /gene="cax"
                     /locus_tag="VFY44_01480"
     CDS             1622..2677
                     /gene="cax"
                     /locus_tag="VFY44_01480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HEX5909130.1"
                     /translation="MPIAVGLELAHASPTLIFLAAALGVIPTAALMGESTEHLAAKTG
                     PGLGGLLNVTFGNAPELIIAFFALREGLQEVVKASIVGSIIGNILLVLGAAMLVGGLK
                     REKQTFNRTAATAQAGMLLLALTALIMPAVFQLVHGGGLPGVSEERVDFGSDLEHLSL
                     GVAIVLMVSYVAGLIFSQRTHKGVFNPYGNEEEEEHEGGWSIKRALISLAVAAVLVGL
                     MSEVLVGSISEASKDIGLSEFFVGVFVVAIVGNAAEHWVAVLVAAKDKMDLAVGIAIG
                     SSAQIALFVAPILVLLSFVVGPNPLALVFNGYELAGLVFAVLIANFVTQDGESNWFEG
                     AQLLALYAVLGLVFFYA"
BASE COUNT          440 a          977 c         1030 g          465 t
ORIGIN      
        1 cttgtccgtc ttggcgtaga cggtgtagaa gctggcgtac gagccgttgg tgatgaagca
       61 cttcgagccg ttgatgacgt acttgtcacc cttcttcacg gccgtggtct tcatgcccga
      121 gacgtcggag ccggcgtccg gctcggtcag gcagaacgag gcgtacagcg gggcctcggt
      181 gagcatgccg aagtacttct tctgcagctc ctcggagccg ccgagggcca gcggcgcggt
      241 ggccaggccg ttcgcgccga gagaggtgcc gatgcccgag cagccccagg agatctcctc
      301 ctcgatgatg gcgccggtca ggtagtccag gcccggaccg ccgtactccg cgggggcgtg
      361 gttgttcatc aggcccagtt cccaggcctt ctcgaagatc tcggcgggcc aggtgccctc
      421 cttgtcgtac tcccaggcct tcgggcggat ctcgttctcc gcgaagtcgt gggccatcgc
      481 ctggatgtcc ttctgctcat cggtaagcgt gaaatcgacc actggtcgtc tccttgggaa
      541 gtgtgtcggc ggcggcgaga gattcagcgc tgagacaggt cgcgcggcgc ccgattgatt
      601 cgtcagtcaa cgctggtacg gacagggtag cccatgggat ggccagtcgc cagcaggccc
      661 tagaacagca cccagaagac catgtagatc ccgccgccga tggtcaggag cgagaacagc
      721 gccaagccca cctgctcggg gtagagcagc gggcgcgagg gcgccatcgt ccgcctgcgt
      781 agccgctcga agagcacgga cctaccctag ggctgtggag ttcgggacgg tgctgatcgc
      841 ggtggtgctg gtggcgatcg tcgtcgcctg cctgagctac atcggcagcg gggcgatcta
      901 caagggcctc ggccgcaccg gcctctcgct cgacgagccc gacctgaggc cggagccggt
      961 ggcgggcagc cccgcggcga acgccgaggc ccaggaggag atccgccaga tgctcgaggc
     1021 gaagtcggcc cggtccgagc gccgtggcga cgggccgatc gacgtggacg ccgagatggc
     1081 ggcgctcagc gcgccgcccg tggccgccga cccggccctg cgcgaggaga tccgccagct
     1141 ggtggtggcc cgcaacgaac gccgggtgcg ccgcggagag gaccccctgg acgtcgacgc
     1201 cgaggtcgac cgcgagctac agcggctgat ataggttgcg cagatggccg acgaccggaa
     1261 attcgagatc gacgagctgc tggtgcggcc cgggacctac ttcaaccccc agaccgaggt
     1321 cctggtggtg gtcgacgact ccacctcgct cgagagcgag atcttcaaca tggaggagtt
     1381 cgagggcgcg gactgggtcc tgatcgccga cgagctcccg gtggacgagg ccaagcgcga
     1441 cgagctgctg gaggacttcc agaccaacgt gcacggcgac gtcgccgtgc gtgccaccac
     1501 cgtcgaggac ccggccggcg aggccgatga ccccgacgtg gacgacgacc caccgcgcgc
     1561 ggtggttccc gtcgacgaag aggagtaggc gctgcgcccc atatacggcc tcggcatctt
     1621 catcccgatc gcggtcgggc tggagctggc ccacgcctcc cccacgctga tcttcctggc
     1681 cgccgcgctg ggcgtgatcc ccaccgcggc gctgatgggc gagtccaccg agcacctggc
     1741 ggccaagacc gggccgggcc tgggcggcct gctgaacgtc acgttcggca acgcgcccga
     1801 gctgatcatc gccttcttcg ccctgaggga ggggctccag gaggtcgtca aggcctcgat
     1861 cgtgggctcg atcatcggca acatcctgct ggtgctgggc gccgcgatgc tggtcggcgg
     1921 cctcaagcgc gagaagcaga ccttcaaccg cacggcggcc accgcgcagg cggggatgct
     1981 gctgctggcc ctgaccgcgc tgatcatgcc cgcggtgttc cagctggtcc acggcggcgg
     2041 cctcccgggc gtcagcgagg agcgggtcga cttcggcagc gacctcgagc acctctccct
     2101 gggcgtggcc atcgtgctga tggtcagcta cgtggcgggg ctgatcttct cgcagcggac
     2161 ccacaagggc gtgttcaacc cctacggcaa cgaagaggag gaggagcacg agggcggctg
     2221 gtcgatcaag cgcgcgctga tctccctggc cgtcgcggcc gtgctggtcg gcctgatgag
     2281 cgaggtgctc gtgggctcga tctccgaggc gtccaaggac atcggcctgt ccgagttctt
     2341 cgtcggcgtg ttcgtggtgg ccatcgtggg caacgccgcc gagcactggg tcgccgtgct
     2401 ggtcgcggcc aaggacaaga tggacctggc cgtgggcatc gccatcggct ccagcgccca
     2461 gatcgcgctg ttcgtggcgc cgatcctggt gctgctctcg tttgtggtcg gccccaaccc
     2521 gctggcactc gtgttcaacg gctacgagct ggccggcctg gtgttcgccg tgctgatcgc
     2581 caacttcgtg acccaggacg gcgagtcgaa ctggttcgag ggcgcgcagt tgctggcgct
     2641 gtacgcggtg ctggggctcg tgttcttcta cgcgtaggct ggcgttttcg ccgctggccg
     2701 caccagctgg ccggcgcaac ggctgggctg gggtagggcg cccggggagg aacacctcac
     2761 ggtccaccgc gacccaagcg ggcgctctgc cgtgggaacg cccaccgagg gacacctcac
     2821 gtcccgccac caccgaacag cgcgccctgc cgtgagaagg cccgcccgtc ccccgcccct
     2881 acccagccca gccgttcgcc gaggccgcag gt
//
LOCUS       DASUPW010000064         3440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_567581, whole genome shotgun sequence.
ACCESSION   DASUPW010000064 DASUPW010000000
VERSION     DASUPW010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567581"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VFY44_01485"
     CDS             complement(<1..330)
                     /locus_tag="VFY44_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909131.1"
                     /translation="MSADQQLRWEADNAPKFAGLAFLTGVLTIAAFAANALTLQGKTG
                     DREILRQIDSHHAGFLASRLLQALVVVALVSSLYYLYKAAVARRPEGGLTMFWPIGVL
                     AAVLLTVA"
     gene            complement(327..518)
                     /locus_tag="VFY44_01490"
     CDS             complement(327..518)
                     /locus_tag="VFY44_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909132.1"
                     /translation="MAPLTPDQVRTRERVETVIRIAAPALNLVLAVGERVSKLVEPED
                     HEYYPPRTSTQGAPPLPPK"
     gene            565..843
                     /locus_tag="VFY44_01495"
     CDS             565..843
                     /locus_tag="VFY44_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909133.1"
                     /translation="MSSEDPFGGLFGDPDELRKRMAELADQMQGAQKVAWADNAIKLA
                     ADMTVAGMGRLNLQGNAEQQTQELRTAMSHLFPEAVALVREAREGFGA"
     gene            851..1771
                     /locus_tag="VFY44_01500"
     CDS             851..1771
                     /locus_tag="VFY44_01500"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX5909134.1"
                     /translation="MPPARTDSHFTSGGLDCAAWLYEPEGPGPHPCVVMAHGFSAVRE
                     QRLDAFAERFAAAGLAALVFDYRHFGASQGEPRQLLSIRRQLEDWRAAVAHARNLAGV
                     DPRRVAVWGSSFSGGHVVAVAAADPGIAAVVSQAPFTDGLSAIAAGGAAQGLKLTGAG
                     LRDATAMLLGREPVYLPAVGPPGTLAVMTAEEAEPGFARIDAPGSTWENRVAGRVALA
                     VGGYRPYAKFGKLRQPVLVQVCEHDDTTPPGPAIKAAERSPNATLIRYPIGHFEIYVD
                     DQFETTVADQTDFLVRNLVGGEVPAAAAPA"
     gene            complement(1768..2769)
                     /locus_tag="VFY44_01505"
     CDS             complement(1768..2769)
                     /locus_tag="VFY44_01505"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX5909135.1"
                     /translation="MLGPATLGESFRSFFDAVDSFFGNLAEVHLGSLFLALVLFGVYL
                     TLRARASFNILRAAYPTETFRFLDIWGAYFAGYGFNAVVPARGGDVVRLFLTKTSVPR
                     SSYPAVAASFTVEMGFDLAMGVLILCFAFTQGVFPKPPDFADLNAFDLSYLASHPRFT
                     LFLLTAIGVASLVAFAMLSMRVKAFWARVRQGLTILYDRDRYLREVFAVQFVGWLFRF
                     AAFWFLLEAFNIGGSVQNVLLVLGVNAVGAAVPFTPQGAGVTQALLVKVFADTAPGAT
                     VAAYSVGQQIALGGFAFGVGFAALFFIFRVRSFKEVIARGREDRAADKVAREAPGAV"
     gene            complement(2773..3234)
                     /gene="bcp"
                     /locus_tag="VFY44_01510"
     CDS             complement(2773..3234)
                     /gene="bcp"
                     /locus_tag="VFY44_01510"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEX5909136.1"
                     /translation="MIEAGQPAPTFTLRNQDDEPVSLDDYKGQKVVLYFYPKADTPGC
                     TTQACGIRDRSGEYAEAGATVIGVSGDEPTKLRKFADKYDLDFTLVGDPDHDVLEAYG
                     VWVEKKNYGKTYMGIQRATFIIGEDGKVAHVIPKASPKTHDDEVLGALAAL"
     gene            complement(3231..>3440)
                     /locus_tag="VFY44_01515"
     CDS             complement(3231..>3440)
                     /locus_tag="VFY44_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909137.1"
                     /translation="EHYEEVIGATVPDKLPMPLQVHRRDGEECPRCGTRIEAIHFKDY
                     VMCYCPKEQTGGRVLKDRRLSRLLK"
BASE COUNT          522 a         1225 c         1232 g          461 t
ORIGIN      
        1 ggccaccgtg agcagcaccg cggcgagcac gccgatcggc cagaacatgg tcaggccgcc
       61 ttcgggccgg cgcgccaccg cggccttgta caggtagtac agggagctga ccagggcgac
      121 cacgaccagc gcctggagca gccggctggc caggaacccg gcgtggtggc tgtcgatctg
      181 gcgcaggatc tcgcggtcgc cggtcttgcc ctgcagcgtc agcgcgttgg ccgcgaacgc
      241 ggcgatcgtc aggactccgg tcaggaaggc caggccggcg aacttggggg cgttgtccgc
      301 ctcccaccgc agctgttggt cggcgctcac ttgggaggca gcgggggcgc gccctgcgtg
      361 gaggtgcgcg gcgggtagta ctcgtggtcc tcgggctcga ccagcttgga cacccgctcg
      421 cccaccgcca acaccaggtt cagggccggc gcggcgatgc ggatcacggt ctcgacccgc
      481 tcgcgggtac gcacctggtc gggcgtcagg ggggccacga ccgagagact agtagacccg
      541 ctgcgtcccc ctacacttga cggcatgtcc tcagaagacc catttggcgg cctgttcggc
      601 gaccccgacg agctgcgcaa gcgcatggcc gagctcgcgg accagatgca gggcgcccag
      661 aaggtcgcgt gggcggacaa cgccatcaag ctggcggccg acatgaccgt ggccggcatg
      721 ggacggctga acctgcaagg caacgccgag cagcagacgc aggagctgcg caccgcgatg
      781 tcgcacctgt tccccgaggc ggtggccctg gtgcgcgagg cgcgcgaggg cttcggcgcc
      841 taggccctcc ttgccgcccg cccgcaccga ctcgcacttc acgtcgggcg ggctcgactg
      901 cgccgcctgg ctgtacgagc ccgagggccc cggcccccac ccctgcgtgg tcatggccca
      961 cggcttcagc gccgtgcgcg agcagcgcct ggacgcgttc gccgagcgct tcgccgccgc
     1021 cggcctggcc gcgctggtgt tcgactaccg ccacttcggc gccagccagg gtgagcctcg
     1081 ccagctgctg agcatccgcc gccagctcga ggactggcgc gccgccgtcg cgcacgcccg
     1141 caacctggcc ggcgtggacc cgcgccgggt cgccgtgtgg gggtcgtcgt tcagcggcgg
     1201 gcacgtggtg gccgtggccg ccgcggaccc cggcatcgcc gcggtggtct cgcaggcccc
     1261 gttcaccgac gggctgtccg cgatcgccgc gggcggcgcg gcccagggcc tcaagctgac
     1321 cggcgccggc ctgcgcgacg ccaccgccat gctgctgggc cgcgagcccg tgtacctgcc
     1381 cgccgtcggc ccgcccggga cgctggcggt catgaccgcc gaggaggccg agccgggctt
     1441 cgccaggatc gacgcgcccg gcagcacctg ggagaaccgc gtggcgggcc gggtcgcgct
     1501 cgcggtgggc ggttaccgcc cgtacgcgaa gttcggcaag ctccgccagc ccgtgctggt
     1561 ccaggtgtgc gagcacgacg acaccacgcc gccgggtccg gccatcaagg ccgcggagcg
     1621 gtcacccaac gccaccctga tccgctaccc gatcggccac ttcgagatct acgtggacga
     1681 ccagttcgag accaccgtgg ccgaccagac cgacttcctg gtgcgcaacc tggtgggcgg
     1741 cgaggtgccg gccgccgccg cgccggccta gaccgcgccc ggcgcctcgc gcgcgacctt
     1801 gtcggccgcc cggtcctccc ggccgcgggc gatgacctcc ttgaagctgc gcacgcggaa
     1861 gatgaagaac agcgcggcga acccgacgcc gaaggcgaac ccgccgagcg cgatctgctg
     1921 gccgaccgag taggccgcca ctgtcgcccc cggggcggtg tcggcgaaga ccttgaccag
     1981 cagcgcctgg gtcacccccg cgccctgggg cgtgaacggc acggcggcgc cgacggcgtt
     2041 gacgccaagg accagcagca cgttctgcac cgagcccccg atgttgaagg cctcgagcag
     2101 gaaccagaag gcggcgaagc ggaacagcca gcccacgaac tggacggcga acacctcgcg
     2161 caggtagcgg tcgcggtcgt acaggatggt caggccctgg cgcacccggg cccagaaggc
     2221 cttgacgcgc atggagagca tcgcgaaggc cacgagcgag gcgacgccga tcgcggtcag
     2281 caggaacagc gtgaagcggg ggtgcgacgc cagataggac aggtcgaacg cgttcaggtc
     2341 ggcgaagtcg ggcggcttgg ggaacacgcc ctgcgtgaac gcgaagcaca ggatcagcac
     2401 ccccatggcc aggtcgaagc ccatctcgac cgtgaagctc gccgccaccg ccggatacga
     2461 ggagcgcggc accgacgtct tggtcaggaa cagccggacc acgtcgccgc cgcgcgcggg
     2521 caccaccgcg ttgaagccgt agcccgcgaa gtaggcgccc cagatgtcca ggaaccggaa
     2581 cgtctcggtc gggtacgccg cgcgcaggat gttgaacgag gcccgggccc ggagggtcag
     2641 gtagaccccg aacagcacca gcgccaggaa cagcgagccc aggtggacct ccgccaggtt
     2701 cccgaagaac gagtcgaccg cgtcgaagaa cgaccggaac gactcgccga gggtggccgg
     2761 gcccagcacg acctagagcg ccgcgagcgc tccgagcacc tcgtcgtcgt gggtcttggg
     2821 cgacgccttg gggatcacgt gggcgacctt gccgtcctcg ccgatgatga acgtggcccg
     2881 ctggatgccc atgtaggtct tgccgtagtt cttcttctcc acccacacgc cgtaggcctc
     2941 gagcacgtcg tggtcggggt cgccgaccag ggtgaagtcc aggtcgtact tgtcggcgaa
     3001 cttgcgcagc ttggtcggct cgtcgccgct gacgccgatc acggtggcgc cggcctcggc
     3061 gtactcgccg gaacggtcgc ggatcccgca cgcctgcgtg gtgcaccccg gcgtgtcggc
     3121 cttggggtag aagtagagga ccaccttctg ccccttgtag tcgtccaggc ttacgggctc
     3181 gtcgtcctgg ttgcggagcg tgaaggtggg ggcgggctgg ccggcttcga tcatttgagc
     3241 aaccttgaca ggcgccggtc cttgagcacc cggccgcccg tctgctcctt cgggcagtaa
     3301 cacatgacgt agtccttgaa gtggatcgcc tcgatgcggg tgccgcagcg ggggcactcc
     3361 tctccgtcgc ggcggtggac ctggagcggc atcgggagct tgtcgggcac cgtcgccccg
     3421 atgacctcct cgtagtgctc
//
LOCUS       DASUPW010000065         3938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_983245, whole genome shotgun sequence.
ACCESSION   DASUPW010000065 DASUPW010000000
VERSION     DASUPW010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983245"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1031)
                     /locus_tag="VFY44_01520"
     CDS             complement(135..1031)
                     /locus_tag="VFY44_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5909138.1"
                     /translation="MTEPAVTVRGLHKSYDSFQAVKGIDLEVRHGEVFAFLGPNGAGK
                     TTTTEILEGYRERDGGEVSVLGVDPARPTREWRSRIGIVLQSAKPEHYLTVRETVAEY
                     ADFYPHPRSVDETIEQVGLTDSAHKRGGKLSGGQQRRLDVALALVGDPDLIFLDEPTT
                     GFDPAARRNAWDMIGGLRDLGKTIFLTTHFMDEAQALADRVAIIRAGAIVAQGPPSEL
                     GGRGRRSFIRFTLPDGVTAADLPVPGRDEAGRAVIEVERPLEPLAAITSWALERGHDL
                     PDLEVERPTLEDVYLELTEEGA"
     gene            1066..1758
                     /locus_tag="VFY44_01525"
     CDS             1066..1758
                     /locus_tag="VFY44_01525"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX5909139.1"
                     /translation="MLKVGHKGADHVAPGNTAASFEAALECGVDMIEFDVLPVPYPDG
                     ELVLAHDYEDAAARTTLTLEEGLDHLAGAAYSDVELDVDLKLPGYEAAVVQALTARAL
                     DERALISSTHPESLRVLGELAPHIRRGWSVPNVRRDYTKNYALMVPAYAVVRWWRSGL
                     PRRAARALRAGECEALMANRLLVSRRLVREVHAAGGQLYVWTVDDPAEIRRLEELGVD
                     AVITNDPRLFDL"
     gene            1812..2327
                     /locus_tag="VFY44_01530"
     CDS             1812..2327
                     /locus_tag="VFY44_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5909140.1"
                     /translation="MAGLSDHTAAESFGDLYERNFDRVYAYVQSMLRDRAAAEDVTSQ
                     AFERAYRKRRGYRPGRGSAEAWLFGIARNAALDELRRRKRHARMATEPEDLEGRPVDD
                     VAETALRRETVRSALERLEPRERELVSLKYMAGLGNTEIAKVVGISESNVSTRLNRTM
                     TKLREVCHDQL"
     gene            2314..3345
                     /locus_tag="VFY44_01535"
     CDS             2314..3345
                     /locus_tag="VFY44_01535"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEX5909141.1"
                     /translation="MTNSDEAFERDLALALAAEAPRARPGFKDELRERVDAGFPRERR
                     FKVPSISRRKLMPALAVATCAVVAVSVVGLNRDERAQDTGMSAPASGGGAAAEQAPDA
                     TVAPSPEPRTDLDFAPGRERRIERSARMSLVAPEDRLEKVGQGIVDVAERYRGYVLSS
                     SLGTGEDEGGGTYELRVPAADLRDALRDLGRLGTVRSQSQNGQDVTAGYVSAADRLQS
                     ARAERRSLLRRLERSGSDTQTEALRRRLELNALRVSAARNQVRELRTRTDYAAVSVTL
                     SSRDDGTVTGGNGDGLGGALDDALGSLSDSVELLVRALGVGIPIGVLALLAWIAARTL
                     RRRRREAIL"
     gene            complement(3342..3827)
                     /locus_tag="VFY44_01540"
     CDS             complement(3342..3827)
                     /locus_tag="VFY44_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909142.1"
                     /translation="MRFGICLAALACALLATATATAATSPNLWATINHCDVAGSSVGI
                     RASMPGNGTSQRMYMRFSAQYRNAAGRYVETGSSTRWIRVGTARTRSVQSGYDFVFAA
                     PGAGSAFTFRGTVGYRWTARKGKRWRVVRTARQVTRGGIRGVEGGVPRGASAATCAIA
                     R"
BASE COUNT          534 a         1423 c         1477 g          504 t
ORIGIN      
        1 gtcgttgccg aacacggtgg cgaagatgac caggaacagc accggcatca gcagcccgaa
       61 gaacaccgcg ggcgggttgc gcaggtagga gatcgtggcg tagcggacct ggcgcgcggc
      121 cagcttcgcc gtgctcacgc gccctcctcg gtcagctcga ggtagacgtc ctccagggtg
      181 ggccgctcga cctccaggtc gggcaggtcg tggccccgct ccagggccca cgacgtgatg
      241 gcggccagcg gctccagcgg gcgctcgacc tcgatcaccg cgcgccccgc ctcgtcgcgc
      301 ccgggaaccg gcaggtcggc cgcggtaacg ccgtcgggca gcgtgaagcg gatgaagctc
      361 cggcggccgc gtccgcccag ctccgagggc ggcccctggg caacgatcgc gccggcgcgg
      421 atgatggcca cccggtccgc cagcgcctgc gcctcgtcca tgaagtgggt ggtcaggaag
      481 atggtcttgc ccaggtcgcg caggccgccg atcatgtccc acgcgttgcg ccgcgccgcc
      541 gggtcgaacc ccgtcgtggg ctcgtccagg aagatcaggt ccgggtcgcc caccagcgcc
      601 agcgccacgt ccagccgccg ctgctggccg cccgacagct tgcccccgcg cttgtgcgcc
      661 gagtcggtca gccccacctg ctcgatggtc tcgtccaccg accgcgggtg cgggtagaag
      721 tcggcgtact cggccaccgt ctcgcgcacc gtcaggtagt gctcgggctt ggccgactgg
      781 agcacgatgc cgatccgcga ccgccactcg cgcgtaggcc gcgccgggtc gacgcccagc
      841 accgagacct cgccgccgtc gcgctcgcgg tagccctcca ggatctcggt ggtggtggtc
      901 ttgccggccc cgttggggcc caggaaggcg aacacctcgc cgtggcggac ctcgaggtcg
      961 atgcccttga ccgcctggaa cgagtcgtag gacttgtgca gcccgcggac ggtcacggca
     1021 ggttcggtca cgccgacgca gactagccct cgtaccctgg tgcccatgct caaggtcgga
     1081 cacaaggggg cggaccacgt cgcgcccggg aacacggccg ccagcttcga ggccgcgctg
     1141 gagtgcggcg tcgacatgat cgagttcgac gtgctgcccg tgccctaccc cgacggggag
     1201 ctggtgctgg cgcacgacta cgaggacgcg gccgcgcgca cgacactgac gctggaggaa
     1261 ggcctggacc acctggccgg cgccgcctac tccgacgtgg agctggacgt ggacctcaag
     1321 ctgcccggct acgaggcggc ggtggtgcag gcgctgaccg cgcgcgccct ggacgagcgc
     1381 gccctgatca gctcgacgca cccggagagc ctgcgcgtgc tgggcgagct ggccccccac
     1441 atccgccgcg gctggtcggt gcccaacgtg cggcgcgact acacgaagaa ctacgcgctc
     1501 atggtgccgg cctacgccgt cgtgcgctgg tggcgctcgg ggctgccgcg ccgggctgcg
     1561 cgcgcgctgc gggccggcga gtgcgaggcc ctgatggcca accggctgct ggtcagccgc
     1621 aggctggtgc gcgaggtgca cgcggccggc gggcagctgt acgtgtggac cgtggacgac
     1681 ccggccgaga tccggcgcct ggaggagctg ggggtcgacg cggtgatcac caacgacccg
     1741 cgcctgttcg atttgtgacg gatcgcgtcc cccgccgtag tggggacgtg aaccaggcga
     1801 caatccagcc gttggcgggg ctgagcgacc acaccgcggc cgaatcgttc ggcgacctgt
     1861 acgagcgcaa cttcgaccgc gtgtacgcct acgtgcagtc gatgctgcgc gaccgggccg
     1921 ccgccgagga cgtgacctcg caggcgttcg agcgggccta ccgcaagcgc cggggctacc
     1981 ggccgggccg cggcagcgcg gaggcgtggc tgttcggcat cgcgcgcaac gccgcgctgg
     2041 acgagctgcg ccggcgcaag cgccacgccc gcatggccac cgagcccgag gacctggagg
     2101 gacggcccgt ggacgacgtg gccgagaccg cgctgcgccg cgagaccgtg cggtcggcgc
     2161 tggagcggct cgagccgcgc gagcgcgagc tggtgtccct gaagtacatg gcggggctgg
     2221 gcaacaccga gatcgccaag gtcgtcggca tctcggagtc caacgtctcg acccgcctga
     2281 accggaccat gaccaagctg cgggaggtct gccatgacca actctgacga agccttcgag
     2341 cgcgacctgg cgctggccct cgcggccgag gccccccggg cccggcccgg gttcaaggac
     2401 gagctgcgcg agcgcgtgga cgccgggttc ccgcgcgagc gccgcttcaa ggtgccctcg
     2461 atctcccgcc gcaagctgat gccggccctg gccgtggcca cgtgcgcggt ggtggcggtg
     2521 agcgtggtgg ggctgaaccg tgacgagcgg gcgcaggaca ccgggatgag cgccccggcc
     2581 tcgggcggcg gcgccgcagc cgagcaggcg cccgacgcca cggtcgcacc ctctcccgag
     2641 ccgcgaaccg acctggactt cgcccccggc cgcgagcgcc gcatcgagcg ctcggcgcgg
     2701 atgtccctgg tcgccccgga ggaccgactg gagaaggtcg gccagggcat cgtggacgtc
     2761 gccgagcgct accgcggcta cgtgctcagc tcctcgctgg gcaccggcga ggacgagggc
     2821 ggcggcacct acgagctgcg cgtgcccgcg gcggacctgc gcgacgcgct gcgtgacctg
     2881 ggccgcctgg gcaccgtccg ctcacagagc cagaacggcc aggacgtgac cgcggggtac
     2941 gtgtcggccg ccgaccggct gcagtccgcc cgtgcggagc gccgcagcct gctgcgccgc
     3001 ctggagcggt cgggcagcga cacccagacc gaggccctgc gccgccggct ggagctgaac
     3061 gccctgcggg tctcggccgc ccggaaccag gtgcgcgaac tgcggacgcg gaccgactac
     3121 gccgcggtct cggtcacgct gagctcccgc gacgacggga ccgtgaccgg cgggaacggc
     3181 gacggcctgg gtggtgcgct ggacgacgcg ctgggctcgc tgtcggactc ggtcgagctg
     3241 ctggtccgcg ccctgggcgt cgggatcccg atcggcgtgc tggcgctgct ggcctggatc
     3301 gcggcacgca ccctgcgccg tcgccggcgc gaggcgatcc tctagcgcgc gatcgcgcag
     3361 gtggcggccg aggcgcccct gggcacgccg ccctcgacgc ccctgatccc cccgcgcgtg
     3421 acctgcctgg ccgtgcggac cacgcgccag cgcttgccct tgcgggccgt ccagcggtag
     3481 ccgaccgtgc cgcggaaggt gaacgcgctg ccggcgcccg gggcggcgaa cacgaagtcg
     3541 tagcccgact gcacggacct cgtgcgggcc gtgccgacgc ggatccagcg ggtgctggag
     3601 cccgtctcca cgtaccggcc cgcggcgttg cggtactggg cggagaagcg catgtacatg
     3661 cgctgggacg tcccgttgcc gggcatgctg gcgcggatcc ccacggacga ccccgccacg
     3721 tcgcagtggt tgatcgtggc ccagaggttg ggggaggtcg cggccgtcgc cgtcgccgtc
     3781 gccaggaggg cgcaggccag cgccgcgaga cagattccga accgcatccg gcgccatggt
     3841 ggcacgggtg cgcggttcgt gtcgcatctc ccagaggtga ccgtatgatc gggcgatgca
     3901 gccgggggag gacacggcag cgcttctcgc gagcgtgg
//
LOCUS       DASUPW010000066         2252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1121972, whole genome shotgun sequence.
ACCESSION   DASUPW010000066 DASUPW010000000
VERSION     DASUPW010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121972"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1828)
                     /locus_tag="VFY44_01545"
     CDS             complement(<1..1828)
                     /locus_tag="VFY44_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909143.1"
                     /translation="MDEVRRFARIAAAGSLVAFLLDALMLSAGHLDLFPDPGVLGGFY
                     DGQGRAMLDGRLAVDPAAAGFEGFVVDGNTYIYFGPVLSLLRMPVLAVTHGLDGRLTL
                     ISMVVGFAALLAAGAWLHWQLRAFLRPGAPIDRFEGAAVFLLQVALGAGAIPLFLASR
                     AVVYHETELWGAALAVGSLAALVMVLRRPSARRIALAGLLVALAINTRVPAGLGGVLA
                     LWGLATMAATGLGLRTERRARTAVALAAAGLVALGSSAVVNVAKFDQPFGIPLDKQVF
                     SGLDQNRKAALAANGGSLFGAKYVPTTLLQAVRPDAVGTTRSLPYVGLPHDRPALVGD
                     VRFDTLERSLSAPTSMPLLVLLALAGLVVAVVRRPLRPLLVLAGAAAAGAGLSLTIAY
                     VTTRYLADFLPFLLLAALIGVQALLPAPAAGGRTARRLVLAAAAVLVVAGIVVNGSVG
                     LVTQRLLSAETAEADRAAFVRTQDRADDLLGRRPAGVGTGAALPALGVPGDLFVLGDC
                     DGLYAAGQFEWVPVERTPRSGVHRLRARLGEGGRPRVLATVGTGAGRVRVVAGPGRRV
                     AVRTARGVAGTGARQALPPGHVAEIGLSFDRLSASTYYLTVSV"
     gene            1886..>2252
                     /locus_tag="VFY44_01550"
     CDS             1886..>2252
                     /locus_tag="VFY44_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5909144.1"
                     /translation="MDESALTERLITYDTSTLEGMQAAAAFVYGWLEARDVEVKGETH
                     NGRPVLAATVGAAQGPTVVFHGHLDVVPGNPEQFTPRVEGDRLIGRGAYDMKGGLAAM
                     MCAVRDLAEQDRVRVHFLCV"
BASE COUNT          332 a          881 c          820 g          219 t
ORIGIN      
        1 ccaccgacac cgtcaagtag taggtgctcg cgctcaggcg gtcgaacgac agcccgatct
       61 cggccacgtg tcccgggggc agggcctggc gggcgcccgt cccggcaacg cccctggccg
      121 tgcgcaccgc gaccctgcgg cccgggcccg cgaccacgcg gacgcggccg gctccggtgc
      181 cgaccgtggc caggacccgt ggccgcccgc cctcgcccag ccgcgcgcgc agccggtgga
      241 cgcccgagcg cggcgtgcgc tccaccggca cccactcgaa ctgcccggcc gcgtacaggc
      301 cgtcgcagtc gcccagcacg aacaggtcgc ccgggacgcc cagggccggc agcgctgcgc
      361 ccgtgccgac ccccgccggg cggcggccga gcaggtcgtc ggcgcggtcc tgggtgcgca
      421 cgaacgcggc ccggtccgcc tccgcggtct cggcgctcag caggcgctgg gtgaccaggc
      481 ccaccgagcc gttcaccacg atccccgcga ccaccagcac cgccgccgcg gccagcacca
      541 ggcgccgggc ggtccttccg ccggcggccg gagcgggcag cagcgcctgg accccgatca
      601 gcgccgccag cagcaggaac ggcaggaagt cggccaggta gcgcgtggtc acgtaggcga
      661 tggtcaggct cagcccggcg ccggcggccg cggcgccggc cagcaccagc agcggccgca
      721 gcggccgccg caccacggcg accaccagcc cggccagggc cagcagcacc agcagcggca
      781 tcgaggtggg ggccgagagg ctgcgctcca gcgtgtcgaa gcgcacgtcg ccgaccagcg
      841 cggggcggtc gtgcgggagg cccacgtagg gcagggagcg ggtggtgccc accgcgtccg
      901 ggcgcacggc ctggagcagg gtcgtgggca cgtacttggc cccgaacagg ctgccgccgt
      961 tggcggccag cgcggccttg cggttctggt cgaggccgga gaacacctgc ttgtccagcg
     1021 ggatgccgaa cggctggtcg aacttggcca cgttcaccac cgcggacgag ccgagcgcca
     1081 ccaggccggc ggccgcgagc gcgaccgcgg tgcgcgcccg ccgctcggtg cgcagcccca
     1141 gccccgtcgc cgccatcgtg gccagccccc acagcgccag gaccccgcca aggcccgcgg
     1201 gcacgcgcgt gttgatggcc agggccacca gcaggcccgc cagcgcgatc cggcgtgccg
     1261 acggccggcg caggaccatc accagcgccg ccagcgagcc caccgccagc gccgcccccc
     1321 acagctcggt ctcgtggtag acgaccgccc tgctggccag gaacagcggg atcgccccgg
     1381 cgcccagcgc cacctggagc aggaagaccg cggccccctc gaagcggtcg atgggcgcgc
     1441 ccggccggag gaacgcgcgc agctgccagt gcagccacgc ccccgcggcc agcagcgcgg
     1501 cgaagccgac caccatcgag atcagcgtca gcctgccgtc caggccgtgg gtgaccgcca
     1561 gcacgggcat gcgcagcagc gacagcaccg ggccgaagta gatgtaggtg ttcccgtcga
     1621 ccacgaaccc ctcgaagccg gcggccgcgg ggtccaccgc caggcggccg tccagcatcg
     1681 cccggccctg gccgtcgtag aagccgccaa gcaccccggg gtccgggaac aggtccaggt
     1741 gcccggcgct gagcatcagc gcgtccagca ggaacgcgac gagggaaccg gccgcggcga
     1801 tgcgggcgaa acgacggacc tcgtccacgc gcggcactct acggctgacc gttcccgccc
     1861 cgcatcccag ctaccatcgc ccgacgtgga tgagagcgct ctcaccgagc gcctgatcac
     1921 ctacgacacg tcgacgctgg aggggatgca ggcggccgcg gcattcgtct acggatggct
     1981 cgaggcccgc gacgtggagg tgaagggcga gacgcacaac ggtaggcccg tgctggccgc
     2041 cacggtgggc gctgcccagg ggccgacggt ggtcttccac ggccacctgg acgtggtccc
     2101 gggcaacccg gagcagttca ccccacgcgt ggagggcgac cggctgatcg gccgcggcgc
     2161 ctacgacatg aagggcggcc tggccgccat gatgtgcgcc gtgcgcgatc tggctgagca
     2221 ggaccgcgtg cgggtccact tcctctgtgt ct
//
LOCUS       DASUPW010000067         3018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1260583, whole genome shotgun sequence.
ACCESSION   DASUPW010000067 DASUPW010000000
VERSION     DASUPW010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260583"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..1397)
                     /gene="gcvPA"
                     /locus_tag="VFY44_01555"
     CDS             complement(51..1397)
                     /gene="gcvPA"
                     /locus_tag="VFY44_01555"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX5909145.1"
                     /translation="MSTYTSATDADRKAMLDAIGVGSLDELFSEIPEAVRLDRPLDIP
                     AGKSESEVFDHLSQLAARNVHADAETTFLGAGMYDHYVPAIIDSLLSRSEFLTPYTPY
                     QPEISQGGLQVMFEFQTAISELTGLPVSNASVYEGPSAVAAAGYLAKLETKRSKFVTS
                     RGLHPHSRDALATHAAGYGMTIDEAGLTAEGATDVDALAALIDDDVAAVFIQQPNFLG
                     TVEDVAKLGEAAKAKGALFIVAADPLPLGILEAPGRQGADIVVGEGQSLGNRLDYGGP
                     SFGFFAASDRFIRKMPGRIAGETRDMDGKRGFVLTLQTREQHIRREKATHNICTAQAL
                     NALAGVVYLSWLGRKGLVDIGELMLKRTHYARETLGLEPINPGPVVREFAVRVPDLDG
                     LFERAREAGINPGYRLGQTYPEYGDGLLIALTERRSRADIDRLASVAATAREEVSA"
     gene            complement(1456..1842)
                     /gene="gcvH"
                     /locus_tag="VFY44_01560"
     CDS             complement(1456..1842)
                     /gene="gcvH"
                     /locus_tag="VFY44_01560"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX5909146.1"
                     /translation="MAESYPEDLKYHPEHDWARIEGDEAVFGITWYAQDALGEVVFYD
                     PPEVGATVTKDQPYTEVESVKAVSDVFAPLSGEVIEVNDALTDTPENINSDPDGDGWL
                     VKVKLSDTAEVESLLDVGAYKDLLQS"
     gene            complement(1850..2920)
                     /gene="gcvT"
                     /locus_tag="VFY44_01565"
     CDS             complement(1850..2920)
                     /gene="gcvT"
                     /locus_tag="VFY44_01565"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX5909147.1"
                     /translation="MSEAVDTLRRTSLFDRHEAAGAKLVPFAGWEMPVQYEGVRQEHL
                     AVRQGAGVFDVSHMGEVVTSGPDAEAYLQHVLSNDITKLADGGAQYSVLCREDGGVID
                     DLFTYRLGDRFLTVTNAANHARDVAWLIEHTEGFDVTVEDAADRYAMLAVQGPEARGI
                     VGALVQGELPARMKTATVGFTGTQVLACGTGYTGEDGVELLVPPDSAPGIWDALVAEG
                     VVPAGLAARDTLRTEVCFHLYGNDLDLEHNPLEAGLGWAVKLDTGFIGSEALRNADTG
                     RKLVAFAFTGPGIPRQGNKVFLDMGEGVVTSGTMSPSLGYGIGMAYVPEDSAEPGTKI
                     EVDVRGKPRTAEVRKKPLYSKE"
BASE COUNT          409 a         1034 c         1095 g          480 t
ORIGIN      
        1 ctcgtagatc gtgatcgtct gctcctcggc gcggggatcc agggcggcca tcaggcggac
       61 acctcctcgc gggcggtggc ggccacggag gccagccggt cgatgtcggc gcggctgcgg
      121 cgctcggtga gcgcgatcag caggccgtcg ccgtactcgg ggtaggtctg gccgaggcgg
      181 tagccggggt tgatgccggc ctcgcgggcg cgctcgaaca gcccgtccag gtcgggcacc
      241 cgcacggcga actcgcgcac gaccgggccc gggttgatgg gctccaggcc cagcgtctcg
      301 cgggcgtagt gggtgcgctt gagcatcagc tcgccgatgt cgaccagccc cttgcggccc
      361 agccaggaca ggtagacgac gccggccagc gcgttcagcg cctgcgcggt gcagatgttg
      421 tgcgtggcct tctcgcgccg gatgtgctgc tcgcgcgtct gcagcgtcag cacgaagccg
      481 cgcttgccgt ccatgtcgcg cgtctcgccc gcgatccggc ccggcatctt gcggatgaag
      541 cggtcggagg cggcgaagaa gccgaacgac gggccgccgt agtccaggcg gttgcccagg
      601 ctctggccct cgccgaccac gatgtcggcg ccttggcggc ccggggcctc gaggatgccc
      661 agcggcagcg ggtcggcggc gacgatgaac agcgcgccct tggccttggc cgcctcgccg
      721 agcttggcca cgtcctcgac ggtgcccagg aagttgggct gctggatgaa caccgcggcc
      781 acgtcgtcgt cgatcagcgc ggccagggcg tccacgtcgg tggcgccctc ggcggtcagg
      841 ccggcctcgt cgatggtcat gccgtaaccc gcggcgtggg tggcaagcgc gtcacgcgag
      901 tgcgggtgca gcccgcgcga ggtcacgaac ttggagcgct tggtctcgag cttggccagg
      961 tagcccgcgg cggccacggc ggacggcccc tcgtacaccg aggcgttgga caccggcagg
     1021 cccgtcagct ccgagatcgc ggtctggaac tcgaacatga cctgcaggcc gccctggctg
     1081 atctcgggct ggtacggcgt gtacggcgtc aggaactccg accgcgacag cagcgagtcg
     1141 atgatcgccg gcacgtagtg gtcgtacatg ccggcgccca ggaacgtggt ctcggcgtcg
     1201 gcgtgcacgt tgcgcgccgc cagctgggac aggtggtcga acacctcgct ctcggacttg
     1261 cccgccggga tgtccagcgg ccggtccagg cggacggcct cggggatctc ggagaacagc
     1321 tcgtccagcg agccgacgcc gatggcgtcc agcatcgcct tgcggtcggc gtctgtggcg
     1381 gaggtgtagg tgctcatatt cggaagttcc tcgtgcggtc ctcgtccgat cgacgatcgg
     1441 actgtggtcc tacgcttacg actgaagcag atccttgtac gcacccacgt ccaggaggga
     1501 ttccacctcg gcggtgtccg acagcttgac cttgaccagc cagccgtcgc cgtcggggtc
     1561 ggagttgatg ttctcgggcg tgtcggtcag ggcgtcgttg acctcgatca cctctccgga
     1621 gaggggcgcg aacacgtcgg agaccgcctt caccgactcg acctcggtgt agggctggtc
     1681 cttggtgacc gtggcgccca cctccggcgg gtcgtagaac acgacctcgc cgagcgcgtc
     1741 ctgggcgtac cacgtgatgc cgaacacggc ctcgtcgccc tcgatccggg cccagtcgtg
     1801 ctcggggtgg tacttcaggt cctcgggata gctctcggcc acgttgctcc tactcctttg
     1861 agtacaaggg cttcttacgc acttcagccg tccgcggctt gccacggacg tcgacctcga
     1921 tcttggtgcc cggctcggcg gaatcctccg ggacgtaggc catgccgatg ccatacccca
     1981 ggctgggcga catcgtgccg ctggtgacca cgccctcgcc catgtccagg aacaccttgt
     2041 tgccctggcg cgggatgccc gggccggtga aggcgaacgc cacgagcttg cggccggtgt
     2101 cggcgttcct gagcgcctcc gagccgatga agccggtgtc cagcttgacc gcccagccca
     2161 ggcccgcctc gagcgggttg tgctcgaggt ccaggtcgtt gccgtacagg tggaagcaga
     2221 cctcggtgcg cagcgtgtcg cgcgcggcca ggcccgcggg cacgacgccc tcggccacca
     2281 gcgcgtccca gatgcccggg gcggagtcgg gcgggaccag cagctcgacg ccgtcctcgc
     2341 cggtgtagcc ggtgccgcag gccaggacct gggtgccggt gaagcccacg gtggcggtct
     2401 tcatccgcgc cggcagctcg ccctggacca gcgcgcccac gatcccgcgc gcctcggggc
     2461 cctgcacggc cagcatggcg tagcggtccg cggcgtcctc gacggtcacg tcgaagccct
     2521 cggtgtgctc gatcagccag gccacgtcgc gggcgtggtt ggccgcgttg gtcacggtga
     2581 ggaaccggtc gcccaggcgg taggtgaaca ggtcgtcgat gaccccgccg tcctcgcggc
     2641 agagcacgga gtactgcgcg ccgccgtcgg ccagcttggt gatgtcgttg gagagcacgt
     2701 gctggaggta ggcctcggcg tccgggccgg aggtgaccac ctcgcccatg tgcgagacgt
     2761 cgaagacgcc cgcgccctgg cgcacggcca ggtgttcctg gcgcacaccc tcgtactgga
     2821 ccggcatctc ccagcccgcg aaggggacga gcttcgcccc cgccgcctcg tggcggtcga
     2881 agagcgaggt acggcgcagg gtgtccacgg cctcggacaa gggacgggga ggttaccgcc
     2941 gggggcgcga cgggctttac gcggcctgtg ttgcgggcgc tacagaaggg ccgcggcccc
     3001 gcgagcaggc tgttccta
//
LOCUS       DASUPW010000068         3852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1815302, whole genome shotgun sequence.
ACCESSION   DASUPW010000068 DASUPW010000000
VERSION     DASUPW010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1815302"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1205
                     /locus_tag="VFY44_01570"
     CDS             63..1205
                     /locus_tag="VFY44_01570"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5909148.1"
                     /translation="MFYDHVTGPWWAELASQVPGLDVIDVHTHIGQNDPDGFTCTTQE
                     LRDGLDTIGARGVVFPMQEPDGYPPANDMVIAEAAASDGQLTPFLRLDPEDAPLAETE
                     RALAAGARGIKLHPRGESFELDHPALTDVYALAGEQRLPVLVHAGRGIPALGHHAVDI
                     CRRNPGLRLILAHACITDLAWIWREAADLPNLFFDTSWWSASDLLALWTMVPPGQVLY
                     ASDAPYSTPGFAAIMNLRCALQAGLSEEQIRLSFGGQTQRLLDREDPVDAGPAPATAV
                     TVDPLLDRVHTFLVAAVGQALSGQEPAELLTLARFACNVGDDAPQAAAAAEVLGLMDM
                     REAFLREGDSDGRPQRFAPGLQLLIAAAVVTRTPAVLSRVPEMQRD"
     gene            complement(1202..1609)
                     /locus_tag="VFY44_01575"
     CDS             complement(1202..1609)
                     /locus_tag="VFY44_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909149.1"
                     /translation="MKIIAMAAEPISPDVLRSAVGDGSDDAEVLVVAPALNSKVRFWV
                     SDPDPAIHRAEDVAKESVDRLEEEGVEAHGETGESDPLLALQDALATFEAEQIVLVTH
                     KGGELNWQEDGLVDDVRERYPDRSVTHLEMERD"
     gene            complement(1606..2664)
                     /locus_tag="VFY44_01580"
     CDS             complement(1606..2664)
                     /locus_tag="VFY44_01580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5909150.1"
                     /translation="MAVADPTTRPEIALLMGRGAETGLINLSELDECASALELSDDEL
                     ESLHQQIDAKGILLTDDCGRQGVMDTHFNLGDLSGTTTDALQLFLNELRRYPLLNKVE
                     EIELAKRIEAGDLEAKERMINSNLRLVVSIAKKYQGQELSLLDLIQEGIFGLIRAAEK
                     FDYRKGFKFSTYATFWIRQAIQRGLANKARTIRIPVHIGQRERKIVRAERDLGARLGR
                     EPTDEEIAKEAEIPVEQVEEVRAAARTVTSLDRPVGEDGDTAFGDLLPNEGPELEEEV
                     TVNLAKELLQRTVEDLPEPERSVIRMRYGIDGEEPKALRETGRQLELSAERVRQLESR
                     ALERLSSHREIEGLRDAA"
     gene            complement(2700..3029)
                     /locus_tag="VFY44_01585"
     CDS             complement(2700..3029)
                     /locus_tag="VFY44_01585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5909151.1"
                     /translation="MTILEVETEDREDGVQLALKGELDLSTIEKVEQELTRAEDGGHR
                     VIVLDLSGLTFLDSTGLRTIVTADQRARRGGRRVVILKGPATVHRVFTITRLDERLEM
                     VDSADEI"
     gene            3086..>3852
                     /gene="otsB"
                     /locus_tag="VFY44_01590"
     CDS             3086..>3852
                     /gene="otsB"
                     /locus_tag="VFY44_01590"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX5909152.1"
                     /translation="MSAPTAETLADTLRPLTADPARAAVFCDIDGTLAPIVSRPEDAH
                     VHESVSRLLAGLARRYGLVACVSGRSAAEARRLVGVGALAYVGSHGAEVAPPGQKEPE
                     LLDAFASWQAKVAGFVAERQEDRELKRLRVRVEDKGPIKAFHWRGAPDEQAAETWLAG
                     VAQEAPEAGLEVHWGRKVLEIRPPVHVSKGDGIRRLLEGGSLRTALYGGDDATDLDAF
                     DALESLVEAGELDAAVKVGVSSDEGPRAIVERADLAV"
BASE COUNT          493 a         1389 c         1384 g          586 t
ORIGIN      
        1 tgggctacat gttcggcggc ccggcctgga agccgccacc gcagcgtcag taatctcacc
       61 gggtgttcta cgaccacgtg accggcccgt ggtgggccga gctggcctcg caggtgccag
      121 gcctggacgt gatcgacgtg cacacgcaca tcgggcagaa cgatcccgac ggcttcacgt
      181 gcacgacgca ggagctgcgt gacggcctgg acacgatcgg cgcccgcggc gtggtgttcc
      241 cgatgcagga gccggacggc tacccgccgg ccaacgacat ggtcatcgcc gaggcggcgg
      301 ccagcgacgg gcagctgacc cccttcctgc ggctggaccc cgaggacgcg ccgctggccg
      361 agaccgagcg ggcgctggcc gcgggggccc ggggcatcaa gctccacccg cggggcgagt
      421 cgttcgagct cgaccacccc gcgctgaccg acgtctacgc cctggccggc gagcagcggc
      481 tgccggtgct ggtgcacgcc ggccgcggga tccccgccct gggccaccac gcggtggaca
      541 tctgccggcg caacccgggc ctgcggttga tcctggcgca cgcctgcatc accgacctgg
      601 cctggatctg gcgcgaggcc gccgacctgc ccaacctgtt cttcgacacc tcgtggtggt
      661 cggccagcga cctgctcgcg ctgtggacca tggtcccgcc cggccaggtc ctgtacgcca
      721 gcgacgcgcc ctacagcacg cccggcttcg ccgcgatcat gaacctgcgc tgcgccctcc
      781 aggcggggct cagcgaggag cagatccggc tctcgttcgg cggccagacc cagcggctcc
      841 tggaccgcga ggacccggtc gacgccgggc cggcgccggc caccgccgtc acggtcgacc
      901 cgctgctgga ccgcgtgcac accttcctgg tggcggcggt ggggcaggca ctgtcgggcc
      961 aggagccggc ggagctgctg accctggccc ggttcgcctg caacgtgggc gacgacgccc
     1021 cgcaggccgc ggcggccgcc gaggtgctgg ggctgatgga catgcgcgag gccttcctac
     1081 gcgaaggcga ctccgacggg cgcccgcagc ggttcgcccc cgggcttcag ctgctgatcg
     1141 ccgccgcggt ggtcacgcgg acgcccgcgg tgctcagtcg cgttcctgag atgcagcgcg
     1201 actagtcgcg ctccatctcc aggtgggtga cgctgcggtc ggggtagcgc tcgcgcacgt
     1261 cgtccacgag gccgtcctcc tgccagttca gctcaccgcc cttgtgggtg accagcacga
     1321 tctgctcggc ctcgaaggtg gcgagcgcgt cctggagggc cagcagcggg tcggactcgc
     1381 cggtctcgcc gtgcgcctcg accccctcct cctccaggcg gtccacgctc tccttggcga
     1441 cgtcctcggc gcggtggatg gccggatcgg ggtccgagac ccagaagcgc accttgctgt
     1501 tcagcgccgg cgccaccacc aggacctcgg cgtcgtcgga accgtcaccg acggccgaac
     1561 gcagcacgtc gggcgagatc ggctcagcgg ccatggcgat gatcttcacg cagcgtccct
     1621 caatccctcg atttcacgat gtgatgacag gcgctcgagc gcccgtgact ccagctggcg
     1681 gacgcgctcg gccgagagct cgagctggcg gccggtctcg cgcagcgcct tcggctcctc
     1741 gccgtcgatg ccgtaacgca tgcggatcac gctgcgctcg ggctcgggca ggtcctcgac
     1801 cgtgcgctgg aggagctcct tggccaggtt gaccgtgacc tcctcctcca gctcggggcc
     1861 ctcgttgggc agcaggtcgc cgaaggcggt gtcgccgtcc tcgcccaccg gccggtccag
     1921 gctggtgacc gtgcgcgccg ccgcgcggac ctcctcgacc tgctcgaccg ggatctcggc
     1981 ctccttggcg atctcctcat ccgtgggctc gcgccccagg cgggcgccca ggtcgcgctc
     2041 tgcgcgcacg atcttgcgct cgcgctggcc gatgtgcacg gggatgcgga tggtccgggc
     2101 cttgttggcc agcccgcgct ggatggcctg acggatccag aaggtcgcgt acgtcgagaa
     2161 cttgaacccc ttgcggtagt cgaacttctc ggccgccctg atcagcccga agattccctc
     2221 ctggatcagg tccagcaggc tcagctcctg gccctggtac ttcttggcga tcgagaccac
     2281 gaggcgcagg ttggagttga tcatccgctc cttggcctcc aggtcgccgg cctcgatgcg
     2341 cttggccagc tcgatctcct cgaccttgtt cagcagcggg tagcgccgga gctcgttcag
     2401 gaacagctgg agcgcgtcgg tggtcgtccc gctcaggtcg ccgaggttga agtgggtgtc
     2461 catcacgccc tggcggccgc agtcgtcggt cagcaggatg cccttggcgt cgatctgctg
     2521 gtggaggctc tccagctcgt cgtcggagag ctcgagcgcc gacgcgcact cgtcgagctc
     2581 ggacaggttg atcaggcccg tctccgcgcc gcggcccatc aggagcgcga tctccgggcg
     2641 tgtcgttggg tcggctactg ccatctgccg atcctacgct tcctggcggc gtgccgggct
     2701 cagatttcgt ccgcgctgtc caccatctcc agccgctcgt ccaggcgggt gatggtgaag
     2761 acgcggtgca cggttgccgg gcccttgagg atcacgacgc ggcgcccgcc gcggcgggcg
     2821 cgctggtcgg ccgtgacgat ggtgcggagg ccggtcgaat ccaggaacgt caggccgctc
     2881 aggtcgagca cgatgacgcg gtgcccgccg tcctcggcgc gggtgagctc ctgctcgacc
     2941 ttctcgatcg tggacaggtc gagctcgccc ttgagagcga gctgcacgcc gtcctcccga
     3001 tcctctgttt ccacctccaa gatggtcacg agggccgagg ctacccggtc ggagcgtcgc
     3061 gcggcgccgc gccatactta ggccgatgtc cgcgccgacc gccgaaacgc tggccgacac
     3121 gctgcgcccc ctgacggccg acccggcgcg cgccgcggtg ttctgcgaca tcgacgggac
     3181 gctggcgccg atcgtgtccc gaccggagga tgcgcacgtg cacgagagcg tctcgcggct
     3241 cctggccggc ctcgcccgca ggtacggcct ggtggcgtgc gtctccggcc gctcggcggc
     3301 cgaggcccgg cgcctggtgg gcgtcggggc gctggcctac gtgggctcac acggggccga
     3361 ggtggcgcct ccgggccaga aggagcccga gctgctggac gcgttcgcca gctggcaggc
     3421 caaggtcgcg gggttcgtgg ccgagcgcca ggaggaccgc gagctcaagc gcctgcgcgt
     3481 gcgggtcgag gacaagggcc cgatcaaggc cttccactgg cgcggcgcgc ccgacgagca
     3541 ggccgcggag acgtggctgg ccggggtggc gcaggaggcg cccgaggcgg gcctcgaggt
     3601 gcactggggc cgcaaggtcc tggagatccg gccgcccgtg cacgtgagca agggcgacgg
     3661 catccgccgc ctgctggaag gcggatcgct gcgcaccgcc ctctacgggg gcgacgacgc
     3721 caccgacctg gacgccttcg acgcgctcga gtccctggtc gaggccggcg agctggacgc
     3781 cgcggtcaag gtcggggtca gctccgacga gggcccgcgg gcgatcgtgg agcgcgccga
     3841 cctggcggtg ga
//
LOCUS       DASUPW010000069         3544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1815419, whole genome shotgun sequence.
ACCESSION   DASUPW010000069 DASUPW010000000
VERSION     DASUPW010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1815419"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..1319
                     /locus_tag="VFY44_01595"
     CDS             102..1319
                     /locus_tag="VFY44_01595"
                     /inference="COORDINATES: protein motif:HMM:NF013504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 6 protein"
                     /protein_id="HEX5909153.1"
                     /translation="MRRPVSALVAALTLLISASAAQAQRPDPLKVPDLFRPCGAATGA
                     GPKIPADPRGVNPGSPNPLAADSFFVDPSEQSAKDLVAYQRAGRKRDAADIAKLALRP
                     KARWLGAYTRPNMPLKVRNYLNCVQVFQPGATPLFTVLRHQGKQCNAGYDGGGVAEDE
                     ATKAWYRDLAEAIGDQRVVIAYEPDSIGTIECLARSRRQARKDLLRYGVDVLSRLPNA
                     TIYLEGTASDWKSARFTASMLRYIGVAKVRGFMLNVTHYDWTSKNIAYGRKVSKLVGG
                     KPFVVSTSYNGRGPVSYTARNKKRINVFCNPRYRGLGPAPTTNTGYAGVDAFLWLNRP
                     GLSGAGACNGGPLPVGSWWPARALMFSRYATDWVTPRNASRFGLSRRFSLCRMGAPVN
                     GSKYSSVAPEKRC"
     gene            1390..2646
                     /locus_tag="VFY44_01600"
     CDS             1390..2646
                     /locus_tag="VFY44_01600"
                     /inference="COORDINATES: protein motif:HMM:NF013504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 6 protein"
                     /protein_id="HEX5909154.1"
                     /translation="MTKTLALLTALLLALLAAPLAPASAQLPDIDCVFAPDLPECEDE
                     EQPEPTPVAPVTPPAQAPQGDTRGVSPAAPNPLLGMSWYVDQSKWVPQWRQYRQYVRS
                     GQDGKAALAAKIALQPQFRWFGRWNESEKGGIAGVMRRYLERVQEEQPGSVPQIVTLR
                     HQGKECNPRYTGGGAAEDARTREWFRDFARGVGDARVVIGYEPDSVGTIGCLARSHRK
                     ARLKLLRYGIDVLSQLPNATIYIEGAASDWTNPRVVAKRLRYIGVAKVRGFMLNVTHY
                     DWTYKNLAYGRKISRLVGGKHFIVSTSFNGRGPVHYRRWLNRKKFKWRRITVWCHPLK
                     RGLGIAPTAQTGDPLADGFFYIGRPGYSGGDCNGGPLPIGSWYPDRALMFARYATTWR
                     GPPRGFRFGFPRGLSLRAVAGDQLQP"
     gene            2711..>3544
                     /gene="ribD"
                     /locus_tag="VFY44_01605"
     CDS             2711..>3544
                     /gene="ribD"
                     /locus_tag="VFY44_01605"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX5909155.1"
                     /translation="MASTTATERDLRHLRRAIELAERARGHTSPNPLVGAVVVRGDTV
                     IGEGFHARAGEPHAERAALAACREDPAGATMYVSLEPCAHTGRTPPCTEAIVESGISR
                     VVVASDDPTAKASGRGLGILRDEGVAVEMVDGEVGQAARLLNQPFRKHARTGRPLVVF
                     KSAMSLDGKVATAGGDSQWISGESSRARAHRWRTECDAVAVGIGTVRADDPLLTSRIE
                     GAGRQPVRVVFDSEAALATGAQLVRTIDQSPVILVCSRAAKRARLDPLRNAGVDVMIT
                     AG"
BASE COUNT          503 a         1339 c         1232 g          470 t
ORIGIN      
        1 agggctacag ccgggtgtcg ccggagaagc gctgcggccg taacagcacg gtcagccggt
       61 aggtccttgc aaacggggcg gcgctggccc attctggccc gatgcgtcgt cccgtctccg
      121 cgctggtcgc ggccctcacc ctgctcatct ccgcgtccgc agcccaggcg cagcgacccg
      181 atccgctgaa ggtccccgac ctgttccgcc cctgcggggc cgccacgggc gccgggccca
      241 agatccccgc cgacccgcgc ggggtcaacc cgggctcgcc caacccgctg gccgccgact
      301 cgttcttcgt cgatcccagc gagcagtcgg ccaaggacct ggtggcctac cagcgggccg
      361 gtcgcaagcg cgacgccgcc gacatcgcca agctggccct ccggcccaag gcgcgctggc
      421 tgggtgcgta cacccgcccc aacatgcccc tgaaggtgcg caactacctg aactgcgtcc
      481 aggtgttcca gcccggggcc acgccgctgt tcaccgtgct gcgccaccag ggcaagcagt
      541 gcaacgccgg ctacgacggc gggggagtgg ccgaggacga ggccaccaag gcgtggtacc
      601 gcgacctggc cgaggccatc ggcgaccagc gcgtggtcat cgcctacgag cccgactcga
      661 tcgggacgat cgagtgcctg gcgcgttcgc gccgtcaagc ccgcaaggat ctgctgcgct
      721 acggcgtcga cgttctatcc aggctcccca acgccaccat ctacctggag ggcaccgcct
      781 ccgactggaa gagcgcgcgc ttcaccgcgt ccatgctgcg ctacatcggc gtggccaagg
      841 tgcgcggctt catgctcaac gtcacccact acgactggac gtccaagaac atcgcctacg
      901 ggcgcaaggt ctcgaagctg gtgggcggca agccgttcgt ggtctcgacc tcctacaacg
      961 gccgcggccc ggtgtcctac acggccagga acaagaagcg catcaacgtg ttctgcaacc
     1021 cgcgctaccg cgggctcggc ccggcgccga ccacgaacac cggatacgcg ggcgtggacg
     1081 ccttcctctg gctcaaccgg cccggtctgt cgggcgccgg cgcgtgcaac ggcggcccgc
     1141 tgcccgtggg cagctggtgg ccggcgcgcg cgctgatgtt cagccgctac gccaccgact
     1201 gggtcacgcc gcgcaacgcc tcgcgcttcg ggctcagccg gcggttctcg ttgtgccgca
     1261 tgggcgcgcc cgtgaacggc agcaagtaca gctccgtggc gcccgagaag cgctgctgac
     1321 ctggcgggtc ggcgctctca agcacggctc gcaggcgccg ataggacatt cgagcaccgt
     1381 cccgacgcca tgaccaagac cctcgccctc ctgaccgccc tgctcctcgc gctgctcgcg
     1441 gcgccgctgg cgcccgcctc cgcccagctc cccgacatcg actgcgtgtt cgcgcccgac
     1501 ctgccggagt gcgaggacga ggagcagccg gagcccacgc ccgtggcccc cgtgacgccg
     1561 ccggcgcagg caccgcaggg cgacacccgc ggggtcagcc ccgccgcccc gaacccgctg
     1621 ctcgggatga gctggtacgt ggatcagtcc aagtgggtgc cgcagtggcg ccagtaccgc
     1681 cagtacgtgc gctcgggcca ggacggcaag gccgcgctgg cggccaagat cgcgctccag
     1741 ccgcagttcc gctggttcgg ccgctggaac gagagcgaga agggcggcat cgccggcgtc
     1801 atgcggcgtt acctcgagcg cgtgcaggag gagcagccgg gctccgtccc gcagatcgtg
     1861 acgctgcgcc accagggcaa ggagtgcaac ccgcgctaca ccggcggcgg ggcggcggag
     1921 gacgcgcgga cgcgcgagtg gttccgcgac ttcgcccgcg gcgtgggcga cgcgcgcgtg
     1981 gtcatcggct acgagcccga ctcggtgggc accatcggct gcctggcccg gtcgcaccgg
     2041 aaggcgcgcc tgaagttgct gcgctacggc atcgacgtgc tgtcccagct gcccaacgcc
     2101 acgatctaca tcgagggcgc cgcgtcggac tggaccaacc cgcgcgtggt ggccaagcgg
     2161 ctgcggtaca tcggcgtggc caaggtgcgc ggcttcatgc tcaacgtcac ccactacgac
     2221 tggacctaca agaacctggc ctacgggcgg aagatctcgc gcctggtggg cggcaagcac
     2281 ttcatcgtct cgacgtcgtt caacggccgc gggccggtgc actaccggcg ctggctgaac
     2341 cgcaagaagt tcaagtggcg ccggatcacc gtgtggtgcc acccgctcaa gcgcgggctg
     2401 ggcatcgccc ctaccgcgca gacgggcgac cccctggccg acggcttctt ctacatcggg
     2461 cggcccggct actccggtgg ggactgcaac ggcgggccgc tcccgatcgg gtcgtggtac
     2521 ccggaccggg cgctgatgtt cgcccgctac gccaccacct ggcgtgggcc gccgcgcggc
     2581 ttccgcttcg gcttcccgcg cggcctgtcg ctgcgggcgg tcgcgggcga ccagttgcag
     2641 ccgtaggcgc gccaccgggc agccggagcc cgtcgggtaa agttgactcg cgagtcaacc
     2701 cgcaccccga atggcctcca ccaccgccac cgagcgtgat ctccggcacc tgcggcgcgc
     2761 catcgagctg gcggagcgcg cccgtgggca cacctcgccc aacccgctcg tgggcgccgt
     2821 cgtggttcgc ggcgacaccg tgatcgggga gggcttccac gccagggcgg gcgagcccca
     2881 cgccgagcgc gcggccctcg ccgcgtgccg cgaggacccg gccggggcca ccatgtacgt
     2941 ctcgctcgag ccgtgcgccc acacgggccg cacgccgccc tgcaccgagg ccatcgtcga
     3001 gtccggcatc agcagggtcg tggtggcctc cgacgatccg accgccaagg cctccggccg
     3061 cgggctgggc atcctgcgcg acgagggcgt cgcggtcgag atggtggacg gcgaggtcgg
     3121 ccaggcggcc cgcctgctca accagccctt ccgcaagcac gcccgcaccg gccgcccgct
     3181 ggtggtgttc aagtcggcca tgtcgctgga cggcaaggtg gccaccgccg gcggcgactc
     3241 gcaatggatc tcgggggagt ccagccgcgc gcgggcgcac cgctggcgca ccgagtgcga
     3301 cgcggtggcg gtcggcatcg gcaccgtccg cgccgacgac ccgctgctga cctcgaggat
     3361 cgagggcgcc gggcgccagc ccgtccgcgt ggtgttcgac agcgaggcgg ccctggccac
     3421 cggcgcccag ctggtgcgga cgatcgacca gtcgccggtg atcctggtct gctcgcgcgc
     3481 cgccaagcgc gctcgcctgg acccgctgcg caacgccggc gtggacgtga tgatcaccgc
     3541 cggc
//
LOCUS       DASUPW010000070         8860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1261213, whole genome shotgun sequence.
ACCESSION   DASUPW010000070 DASUPW010000000
VERSION     DASUPW010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8860
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261213"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VFY44_01610"
     CDS             <1..423
                     /locus_tag="VFY44_01610"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEX5909156.1"
                     /translation="AREEHGLELLRAMEADWPFFSALLSNAEMACSKADAGIAARYFD
                     LWDDDEARERIRSAVEGEFDRTRRELAGVLDVDELLDRHPVLQASIARRNPYVDPLSY
                     VQIELLRRLRDGDGDDEALGRVSLLTVNGIAGGLRNTG"
     gene            complement(427..1707)
                     /locus_tag="VFY44_01615"
     CDS             complement(427..1707)
                     /locus_tag="VFY44_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEX5909157.1"
                     /translation="MATALPLKNPLDNITYEDLYRRWEKGNWQAMAIDFSQDAVDWRE
                     KFTDIERTGALWNYSLFFWGEDAVADGLSPYIDAAPTEEQKYFLTTQQVDEARHAVFF
                     NRFMKDVVGIGGDRVSDGLNDIEPQLTWGFTKVFDRLVDMSDELRRDRSVPKLAAAVT
                     LYHMVIEAALAQPGQHFITSYLTDRGVMPGFREGMENVAADEQRHIGFGVKLLSDLRK
                     MDPEVPYAVADLLREVLPWTAAVLVPPNWDMRYIESFGFTIEDIGEEGASSLETKLRS
                     AGMPIDELPGPPVFPVDGTPRERAARGQQMVRAGLLGEKVGPPSSDPRDMAMLFEVIA
                     SSLETEKAKGPGVLQWEFTDGAPAWHVVVANGDTRAVQGRADAPRVTFKTSWEDFVDM
                     SMGRTDMHRLLLRGRFRPKGDLRWLVGSRGMFPG"
     gene            1728..2339
                     /locus_tag="VFY44_01620"
     CDS             1728..2339
                     /locus_tag="VFY44_01620"
                     /inference="COORDINATES: protein motif:HMM:NF037009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX5909158.1"
                     /translation="MTQRMYQKGEERRESILRATLRVIGAHGADAVTHRAVAAEAGVP
                     LSATTYYFDSKEQLLQQSLLMAAREETERIERVVLDLAPRSLGVGEWAEAVSEVVGRS
                     LRDEAATQVALFELGLEATRTPALRQELADWQSALHGLAAMGCRAVGSDEPELDGTLV
                     VAAIAGLMLQGLAADVRDFEGTVLRPALERLFARIAAGERLPV"
     gene            2415..3149
                     /locus_tag="VFY44_01625"
     CDS             2415..3149
                     /locus_tag="VFY44_01625"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5909159.1"
                     /translation="MHGYPSSSFMWRRPMDALAEAGWRAVAPDLPGYGDSEPDPPATW
                     ERQIEALERFRSGLGLEDVLLVVHDWGGLIGLRWACDHPGAARALAISDTGFFPDGRW
                     HGMAKALRTEGEGEQFMDNVSRDLLGMALAQISPGMTDADVDECWKCLSTPERRAGVL
                     ELYRSGDFEKLEPYQGKLAALGIPTRLIWAADDEFAPVAGAHRLAAEIPGAEVAVIEG
                     ARHFLVEDAPERYCEELVAFARSLSA"
     gene            complement(3104..3763)
                     /locus_tag="VFY44_01630"
     CDS             complement(3104..3763)
                     /locus_tag="VFY44_01630"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5909160.1"
                     /translation="MNQELTAGAWLENPVTGELARMNVGPADTGGRRVEADLWVQPGG
                     AVAGAHVHEHFLERFQVLEGEVGFQVAGSERTACAGDAPVEVPAGVVHDWWNAGAVVA
                     HVRVQVEADPAAVGRPAARFVSMIEAAWSLGALGHVNAKGMPTPLWLAAVAREYADVI
                     RFVRPPAFVQAALFGPLAAIARRSGRDPLAPELHGTGAACLIPAPQAESERAKATSSS
                     Q"
     gene            3817..4290
                     /locus_tag="VFY44_01635"
     CDS             3817..4290
                     /locus_tag="VFY44_01635"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5909161.1"
                     /translation="MQAKFMPSGPPVGTDNFGNLLREPGRVMQDLVAQEVARRGFEDL
                     RPALLAVGQHVRADGSRVTELAELSMLTKPTVVHMVDELERLGYVERRPDPADGRAKL
                     VVLTGRGREAEAAGREAIAQIHEAWTALIGEPAMARLEADLRTLRDALWPPARAR"
     gene            4303..5640
                     /locus_tag="VFY44_01640"
     CDS             4303..5640
                     /locus_tag="VFY44_01640"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX5909162.1"
                     /translation="MDTLFEEESEPPAGQTPVRKGDQPLAARLRPETLDEYVGQDHLL
                     EPGSALRTALDEGRPHSMILFGPPGTGKTTLARLIAVNAAAAFEELSAVNAGRQQVRD
                     VMERAEHRRKTGGQQTILFLDEIHRFNKAQQDVLLPAVEEGLITLVGATTENPYFEVN
                     SALLSRCRVYELMRLDDAQVVELLRRALSHERGLADPPEAEDEALEFLAARSGGDART
                     ALAALELAADTVEKGAKVDMALAEDALQRRAVLYDKGGDIHYDTVSAWIKSTRGSDPD
                     ASLLYLAVMIEGGEDPRFIARRMVILASEDIGNADPQALTVAVSAAHAVEHVGMPECA
                     HNLAQATVYLSLAPKSNASYKALGHARHWVRQHGAPIPPPPLRDSSYAGAEKLGRGLD
                     YDYPHDHPEGVSDQELMPADAQGEQFLELSDHGFEKDLAERWERILKARGRTP"
     gene            5743..6312
                     /locus_tag="VFY44_01645"
     CDS             5743..6312
                     /locus_tag="VFY44_01645"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5909163.1"
                     /translation="MTEVGEHTFLADPGALMATTHEVGDGLRVRLRLARPSDTPRVRE
                     FLEGLSAETRHRRFFTAMPSVPEHVVRHFTFYNPRERMIVVAVALIDGRERILGLADV
                     VLLETGVAELAVVVDDGLQKRGVGTLLTEVIASLAVRQGATHLYAELLDDNEPMRRLM
                     ERLGHTVRTVEDGTAQLYTRLAATQRRAA"
     gene            6323..7102
                     /locus_tag="VFY44_01650"
     CDS             6323..7102
                     /locus_tag="VFY44_01650"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX5909164.1"
                     /translation="MPSHTERSTLAACVATVIGVPTAEVPLGDVDLRAWLAGRGLGLV
                     PVGDPAAFEWAGPWIALRPAANGSGPLAAVMFGVPSGVIWDPAGTTDEVLGGFMVAPL
                     DVSVWEPPSAREPGRGVVEALVLAPDAEAPVRLVEEAAALPGRGLEGDRYADDSGTFR
                     SGRPGSALTLIEAGVLDELGPIDHRRNVVVRGTDLNALVGRDFTVGEVRCRGSRLCEP
                     CLHLDKLNDGGVLRPLVHRGGLRADIVEGGTVHVGDVLLPL"
     gene            7099..8676
                     /locus_tag="VFY44_01655"
     CDS             7099..8676
                     /locus_tag="VFY44_01655"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5909165.1"
                     /translation="MTPATATDQLESFSPFDGSRLGAVPTITPDQVQGVVDDVARIQP
                     FWADLPLAERARTMRRTAQVIIDRLDELTELLSREQGKPRNESYTMELFPTIDACHWI
                     AAEGQKLLRDERISMPLFAKQKRARFTYEPLGVVGVIAPWNYPWTIPFSEVAIALMCG
                     NGVVLKPASLTPLIGEQIQRVFERAGVPEGLIRTVHGGGAVGGALVESSAKKIFFTGS
                     VEVGRGVGVACAERMKGSVLELGGKDPAIVCSDAHLGNAVSGITWGGFANAGQTCSGI
                     ERVYVMRDVAEPFTEALVAKAKGLTVGDPMSWDTEIGPMVSRDQLELVDELVQDAVAN
                     GAELRCGGKVQPGGVPEADYYAPTVLTNVTHDMRIMREEIFGPVVPVITVDSEDEAIT
                     LANDSEFGLGASVWTADREHGERLAHQIDSGMVWTNDHMFSHGVCSCAWGGTKDSGLG
                     RSHSKFGFYECVSIKTLAYEPGLTRNLWWHPYDRALGKAMHSTAQLLYGRDDDKGKAL
                     RGGARSLAKVAARAVRR"
BASE COUNT         1239 a         3211 c         3204 g         1206 t
ORIGIN      
        1 gcgcgcgagg agcacgggct cgagctcctg cgggcgatgg aggccgactg gccgttcttc
       61 tccgcgctgc tgtccaacgc cgagatggcg tgctcgaagg ccgacgccgg catcgcggcg
      121 cgttacttcg acctctggga cgacgacgag gcacgcgagc ggatccggtc cgcggtcgag
      181 ggcgagttcg accgcacccg gcgggagctg gccggcgtgc tggacgtcga cgagctgctt
      241 gaccgccacc ccgtgctcca ggcgtcgatc gcccgccgca acccgtacgt ggaccccctg
      301 tcctacgtgc agatcgagct gctgcggcgc ctgcgcgacg gcgacgggga cgacgaggcg
      361 ctgggccggg tctcgctgct gaccgtcaac gggatcgcgg gcggcctgcg caacacgggc
      421 tgagtcctag cccgggaaca tcccccgcga gcccacgagc cagcgcaggt cgcccttggg
      481 gcggaagcgg ccgcgcagga gcagcctgtg catgtcggtc cgccccatcg acatgtccac
      541 gaagtcctcc caggaggtct tgaacgtgac cctcggcgca tcggcgcggc cctggaccgc
      601 gcgggtgtcg ccgttggcca cgaccacgtg ccacgccggc gcgccgtcgg tgaactccca
      661 ctggagcacc ccggggccct tggccttctc ggtctccagc gacgaggcga tgacctcgaa
      721 cagcatggcc atgtcccgcg ggtcgctcga gggcgggccg accttctcgc ccagcaggcc
      781 cgcgcggacc atctgctgcc cgcgcgcggc acgctcacgc ggggtgccgt cgaccgggaa
      841 cacgggcggc ccgggcagct cgtcgatcgg catgccggcg gagcggagct tggtctccag
      901 cgacgatgcg ccctcctcgc cgatgtcctc gatcgtgaag ccgaacgact cgatgtagcg
      961 catgtcccag ttcggcggga ccagcaccgc ggcggtccac ggcagcacct cgcgcaacag
     1021 gtcggccacc gcgtacggga cctcggggtc catcttgcgc aggtccgaca gcagcttcac
     1081 gccgaagccg atgtggcgct gctcgtccgc cgcgacgttc tccatgccct cgcggaagcc
     1141 gggcatgacg ccgcggtcgg tcaggtagct ggtgatgaag tgctggccgg gctgggccag
     1201 ggccgcctcg atgaccatgt ggtagagcgt gaccgccgcc gccagcttcg ggaccgagcg
     1261 gtcgcggcgc agctcgtcgg acatgtcgac caggcggtcg aagaccttgg tgaagcccca
     1321 cgtcagctgc ggctcgatgt cgttcaggcc gtcggagacg cggtcgccac cgattccgac
     1381 gacgtccttc atgaaccggt tgaagaacac ggcgtggcgc gcctcgtcca cctgctgggt
     1441 ggtcaggaag tacttctgct cctcggtcgg cgccgcgtcg atgtagggcg acaacccgtc
     1501 cgcgacggcg tcctcgcccc agaagaacag cgagtagttc cacagcgcgc cggtgcgctc
     1561 gatgtccgtg aacttctcac gccagtcgac ggcgtcctgg gagaagtcga tggccatcgc
     1621 ctgccagttg cccttctccc agcggcggta gaggtcctcg taggtgatgt tgtcgagcgg
     1681 gttcttgagc gggagcgccg tggccattct ggtacctttg ttccggtttg acacagcgaa
     1741 tgtaccagaa gggcgaggag cgcagggaat cgatcctgcg cgccaccctg cgcgtgatcg
     1801 gcgcccacgg cgccgacgcg gtcacccacc gcgccgtggc cgccgaggcc ggcgtgccgc
     1861 tgtcggccac cacctactac ttcgactcca aggagcagct gctccagcag tccctgctga
     1921 tggccgcgcg cgaggagacc gagcggatcg agcgcgtggt gctggacctg gccccgcgct
     1981 cgctgggcgt gggcgagtgg gccgaggcgg tgtccgaggt cgtcggccgc agcctgcgcg
     2041 acgaggccgc cacccaggtc gcgctgttcg agctcggcct cgaggccacc cgcaccccgg
     2101 ccctgcgcca ggagctggcc gactggcagt cagcgctgca cggcctggcc gccatgggct
     2161 gccgcgcggt gggctccgac gagccggagc tggacggcac gctcgtggtc gccgcgatcg
     2221 cggggctgat gctgcagggg ctcgctgccg acgtacggga cttcgagggc accgtcctgc
     2281 gtcccgcgct cgagcggctg tttgcgcgca tcgccgccgg cgagcgactg ccggtgtagg
     2341 gtcggccgat cgcagagcac gacgggatcg cctaccgctc agccggcccc gacgacggcc
     2401 cggcggccct gctggtgcac ggctacccgt cgtcgtcgtt catgtggcgg cgcccgatgg
     2461 acgcgctggc cgaggccggc tggcgcgccg tggcgcccga cctgccgggc tacggcgact
     2521 ccgagcccga cccgcccgcc acgtgggagc gccagatcga ggcgctcgag cggttccggt
     2581 cgggcctggg cctggaggac gtgctgctgg tggtgcacga ctggggcggg ctgatcggcc
     2641 tgcgctgggc ctgcgaccac cccggcgcgg cccgcgcgct cgccatctcc gacaccggct
     2701 tcttccccga cggcaggtgg cacggcatgg ccaaggcgct gcgcaccgag ggcgagggcg
     2761 agcagttcat ggacaacgtg agccgcgacc tgctgggcat ggcgctcgcg cagatctccc
     2821 ccggcatgac cgacgccgac gtggacgagt gctggaagtg cctgtccaca cccgagcgcc
     2881 gcgccggcgt gctggagctg taccgctcgg gtgacttcga gaagctggag ccctaccagg
     2941 gcaagctcgc cgccctgggc atcccgacca ggctgatctg ggctgccgac gacgagttcg
     3001 cgcccgtggc cggcgcccac cgcctggccg ccgagatccc cggcgccgag gtggccgtga
     3061 tcgagggcgc ccgccacttc ctggtggagg acgcccccga gcgctactgc gaggagctgg
     3121 tggccttcgc gcgctcgctc tcggcctgag gcgccgggat caggcacgcc gcgcccgtgc
     3181 cgtgcagctc gggagccagc ggatcgcggc cgctacggcg ggcgatcgcc gccagcgggc
     3241 cgaacagcgc ggcctgcacg aacgccggcg gccgcacgaa ccggatcacg tcggcgtact
     3301 cgcgggcgac cgcggccagc cagagcggcg tgggcatgcc cttcgcgttg acgtgaccga
     3361 gcgccccgag cgaccacgcg gcctcgatca tcgacacgaa gcgggcagcg ggacggccca
     3421 ccgcggcggg gtccgcctcc acctggacgc ggacgtgggc gaccacggcg ccggcgttcc
     3481 accagtcgtg caccacgccc gccggcacct ccaccggcgc gtcgcccgcg cacgcggtcc
     3541 gctcggagcc cgcgacctgg aagccgacct cgccctcgag cacctggaac cgctcgagga
     3601 agtgctcgtg cacgtgggcg ccggccaccg cgccgccggg ctggacccag aggtcggcct
     3661 ccacgcgccg gccgccggtg tccgccgggc ccacgttcat ccgggccagc tcgccggtga
     3721 ccgggttctc gagccacgcc ccggcggtca actcctggtt catgtcttcc tctctcgtta
     3781 gactctcgaa ctattattat tcgatcatcg aacgaattgc aagcgaagtt catgcccagc
     3841 ggcccacccg tgggcaccga caacttcggc aacctgctgc gtgagccggg ccgggtgatg
     3901 caggacctcg tggcccagga ggtcgcccga cgcggcttcg aggacctgcg cccggcgctc
     3961 ctcgcggtgg gccagcacgt acgggccgac gggtcgcgcg tgaccgagct cgcggagctg
     4021 tcgatgctca ccaagccgac ggtcgtccac atggtcgacg agctggagcg gctgggatac
     4081 gtcgagcgcc ggccggaccc cgcggacggg cgcgcgaagc tcgtcgtcct gaccgggcgc
     4141 gggcgcgagg ccgaggcggc gggccgcgag gccatcgccc agatccacga ggcctggacg
     4201 gccctgatcg gcgagccggc gatggcccgg ctcgaggccg acctgcgcac gctgcgcgac
     4261 gcgctctggc ccccggcccg ggctcgctga cagactgacc ccgtggacac gctgttcgag
     4321 gaggagtccg agccgcccgc ggggcagacg ccggtccgca agggcgacca gccgctggcc
     4381 gcccggctac ggcccgagac gctggacgag tacgtgggcc aggaccacct gctggaaccg
     4441 ggatcggcgc tgcggaccgc gctggacgag ggccgcccgc actcgatgat cctgttcggc
     4501 ccgcccggca ccggcaagac cacgctggcc aggttgatcg ccgtgaacgc cgcggccgcg
     4561 ttcgaggagc tgtcggcggt gaacgccggc cgccagcagg tccgtgacgt catggagcgg
     4621 gccgagcacc ggcgcaagac cggcggccag cagacgatcc tgttcctgga cgagatccac
     4681 cgcttcaaca aggcccagca ggacgtgctg ctgccggcgg tcgaggaggg cctgatcacg
     4741 ctggtcggcg cgaccaccga gaacccctac ttcgaggtca actcggcgct gctctcacgc
     4801 tgccgcgtgt acgagctgat gcgcctggac gacgcccagg tggtcgagct gctgcgccgt
     4861 gcgctgagcc acgagcgcgg gctggccgac ccgcccgagg ccgaggacga ggcgctggag
     4921 ttcctggccg cccgctccgg cggcgacgcc cgcaccgcgc tggcggcgct ggagctggcc
     4981 gcggacaccg tcgagaaggg cgccaaggtg gacatggccc tggccgagga cgccctgcag
     5041 cggcgcgccg tcctctacga caagggcggc gacatccact acgacacggt ctcggcctgg
     5101 atcaagtcca cccgcggatc ggacccggac gcgtcgctgc tgtacctggc cgtgatgatc
     5161 gagggcggcg aggacccgcg cttcatcgcg cggcgcatgg tcatcctggc cagcgaggac
     5221 atcggcaacg ccgaccccca ggcgctgacc gtggcggtgt cggccgcgca cgcggtagag
     5281 cacgtgggca tgcccgagtg cgcgcacaac ctggcccagg ccaccgtgta cctgtccctg
     5341 gcgccgaagt cgaacgcctc ctacaaggcg ctcggccacg cccgccactg ggtacgccag
     5401 cacggggccc cgatcccgcc gccgccgctg cgcgactcct cctacgccgg cgccgagaag
     5461 ctgggccgcg gcctggacta cgactatccg cacgaccatc ccgagggcgt ctccgaccag
     5521 gagctgatgc ccgcggacgc ccagggcgag cagttcctgg agctcagcga ccacgggttc
     5581 gagaaggacc tcgcggagcg ctgggagcgg atcctcaagg cccgcggccg caccccgtag
     5641 ccgccgcaac atccccgcaa atacggtgtt tcctgcaaca gatgttgcgg ttatgtcaag
     5701 cgacattggt ctcaagccga tgccgtctcc tacggtgatc atgtgaccga agtaggcgaa
     5761 cacacgttcc tggccgatcc aggggccctg atggccacca cccacgaggt gggggatggc
     5821 ctgcgcgtgc gcctgcgcct ggcccgcccc agcgacacgc cgcgggtgcg cgagttcctg
     5881 gagggcctgt cggcggagac ccgccaccgg cgcttcttca ccgccatgcc ctcggtgccc
     5941 gagcacgtgg tgcgccactt caccttctac aacccgcgcg agcggatgat cgtggtggcc
     6001 gtcgcgctga tcgacggccg cgagcgcatc ctgggcctgg ccgacgtggt gctcctggag
     6061 accggcgtcg ccgagctggc cgtggtggtg gacgacggcc tgcagaagcg gggggtcggc
     6121 acgctgctga ccgaggtcat cgcctcgctg gccgtgcgcc agggcgcaac gcacctgtac
     6181 gccgagctgc tggacgacaa cgagcccatg cgccggctga tggagcgcct gggccacacg
     6241 gtccgcaccg tcgaggacgg caccgcccag ctgtacaccc gcctggccgc aacgcagcgc
     6301 cgcgccgcct agcctccccc gcgtgcccag ccacaccgag cgttccaccc tggccgcgtg
     6361 cgtggccacc gtgatcggcg tgcccaccgc cgaggtgccg ctgggcgacg tcgatctgcg
     6421 cgcctggctc gccgggcgcg ggctcgggct ggtgcccgtc ggcgaccccg ccgccttcga
     6481 gtgggccggg ccgtggatcg cgctgcgccc ggccgccaac ggctccggcc ccctcgccgc
     6541 ggtcatgttc ggtgtgccct ccggcgtcat ctgggacccg gccggcacca ccgacgaggt
     6601 gctcgggggc ttcatggtcg cgcccctgga cgtgtccgtg tgggagccgc cgtcggcgcg
     6661 ggagccgggc cggggcgtgg tcgaggcgct ggtgctggcg ccagacgccg aggccccggt
     6721 gcggctggtc gaggaggccg ccgccctgcc cgggcggggc ctggagggcg accgctacgc
     6781 ggacgacagc ggcaccttcc gctccggccg cccgggctcg gcgctgacgc tgatcgaggc
     6841 gggggtgctg gacgagctgg gcccgatcga ccaccgccgc aacgtggtgg tgcgcggcac
     6901 cgacctgaac gccctggtgg gacgcgactt cacggtcggg gaggttcgct gccggggcag
     6961 ccggctgtgc gagccgtgcc tgcacctgga caagctcaac gacggcggcg tgctgcggcc
     7021 gctggtgcac cgcggaggcc tgcgggcgga catcgtggag ggcggaacgg tccacgtggg
     7081 tgacgtactg ttgcccctat gaccccagcg accgccaccg atcagctcga gtccttcagc
     7141 cccttcgacg gctcccgcct cggcgccgtg cccacgatca cgccggacca ggtgcagggc
     7201 gtggtggacg acgtcgcgcg gatccagccg ttctgggccg acctgccgct ggccgagcgc
     7261 gcccggacga tgcggcgcac cgcccaggtc atcatcgacc ggctggacga gctgaccgag
     7321 ctgctctccc gcgagcaggg caagccccgc aacgagtcgt acacgatgga gctgttcccc
     7381 accatcgacg cgtgccactg gatcgccgcc gagggccaga agctgctgcg cgacgagcgc
     7441 atctcgatgc cgctgttcgc caagcagaag cgcgcccgct tcacctacga gccgctgggc
     7501 gtggtcggcg tgatcgcgcc gtggaactac ccgtggacga tcccgttcag cgaggtcgcc
     7561 atcgcgctca tgtgcggcaa cggggtggtg ctcaagcccg cgtcgctgac gccgctgatc
     7621 ggcgagcaga tccagcgggt gttcgagcgc gccggcgtgc ccgagggcct gatccgcacc
     7681 gtccacggcg gcggagcagt gggcggcgcc ctggtggagt cgtccgccaa gaagatcttc
     7741 ttcaccggct cggtcgaggt cggccgcggc gtcggggtcg cctgcgccga gcgcatgaag
     7801 ggctccgtgc tggagctggg cggcaaggac ccggcgatcg tctgctcgga cgcccacctg
     7861 ggcaacgcgg tctcgggcat cacctggggc ggcttcgcca acgccggcca gacctgctcg
     7921 ggcatcgagc gcgtgtacgt gatgcgcgac gtggccgagc cgttcaccga ggcgctggtg
     7981 gccaaggcga agggactcac cgtcggcgac ccgatgagct gggacaccga gatcggcccg
     8041 atggtctcgc gcgaccagct tgagctggtc gacgagctcg tgcaggacgc cgtggccaac
     8101 ggagccgagc tgcgctgcgg cggcaaggtc cagccgggcg gcgtgcccga agccgactac
     8161 tacgcgccga ccgtcctgac gaacgtgacg cacgacatgc ggatcatgcg cgaggagatc
     8221 ttcggcccgg tcgtgcccgt gatcaccgtc gactccgagg acgaggcgat cacgctggcc
     8281 aacgactccg agttcggcct gggcgcgtcc gtgtggacgg ccgaccgcga gcacggcgag
     8341 cggctggcgc accagatcga ctccggcatg gtctggacaa acgaccacat gttcagccac
     8401 ggcgtctgct cctgcgcctg gggcggcacc aaggactcgg gcctgggccg ctcgcactcc
     8461 aagttcggct tctacgagtg cgtctcgatc aagaccctgg cctacgagcc cgggctgacc
     8521 cgcaacctct ggtggcaccc gtacgaccgc gcgctgggca aggcgatgca ctccaccgcg
     8581 cagctgctgt acggccgcga cgacgacaag ggcaaggccc tgcgcggcgg cgcgcggtcg
     8641 ctggccaagg tcgcggcgcg ggccgtgcgg cgatgacgca gtcggggcgt tcgttggcag
     8701 tggcgctggc cgcgctggcg ctcgccctcg cagccaccgg cccggctgcg gccggaacgg
     8761 ggcgatgggg tcccccggtg aagctcaacg actacggctg ggcgagatac gtgcacgtgg
     8821 ccgacgacgg tgccgtcacc gtggtcgcgg gatccttccc
//
LOCUS       DASUPW010000071         3601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_846545, whole genome shotgun sequence.
ACCESSION   DASUPW010000071 DASUPW010000000
VERSION     DASUPW010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846545"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1299)
                     /locus_tag="VFY44_01660"
     CDS             complement(127..1299)
                     /locus_tag="VFY44_01660"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HEX5909166.1"
                     /translation="MTEPVYRRREFVGAAATLALGAVFWKEVAAGGEATSRPPAQAYG
                     TYGRPNADGVAVPDGFTVRRVARGTERVPGTGYDWHKASDGSATFPTRDGGWILVSNS
                     EEQRGGAGAMRFGPRGQIEDAYRILDGTTQNCAGGPTPWATWLSCEEFEEGRVWECDP
                     TGRSKARVHDAMGVFKHEAAAVDPRGRHVYMTEDLIDGAFYRYTPRRWPSLAEGRLEM
                     AKVARGGAVEWVDVPDRFARRTPIRRQVKGITEFGRAEGIWFDSGTVYLSTTADSRIH
                     AYDTRRGRIEVIYDGLASRDAPLTRVDNLTASRAGELFVCEDISTEEIHIGLITPRRK
                     ASRFLAVSGGNHVGSELTGIAFNPAGDRFYFSSQRANDLKGEVYEVSGPFRGRRRA"
     gene            complement(1350..3539)
                     /gene="cofH"
                     /locus_tag="VFY44_01665"
     CDS             complement(1350..3539)
                     /gene="cofH"
                     /locus_tag="VFY44_01665"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: protein motif:HMM:TIGR03551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HEX5909167.1"
                     /translation="MRRVTFSRNYTVSLSRTCRCYCKYCAFATHQAHLYDPDEVEGLL
                     DEAVKRNAKELLVLTGEKPEVNPEVARRLESYGHEDFTSYVAWTCERALERGMLPHTN
                     LGVLSRRDLARLREVTASQGLMLESVNPDLVVHQGSPTKHPDVRLQTIRDAGELKIPF
                     TSGILVGIGETPAQRIEALEALAALHAEHGHLQEVILQNFVPHPRYYGAEPALIADDA
                     QRSQTAGTAAPMPSWGQDVGLDEMRALVRASLELMPDVGVQIPPNLAEWWLPLVEEGA
                     TDLGGLSANGDHISPEHPFPTPHRMRKDLQPRGYALTERLCVYPQYMDSEWMEQGVLD
                     VVKTSFWSFIPRKGSGRREDRPIHSELSPGAIERARRGEELEPEELTALFAETRPEVI
                     EDMRQAADELRAELAGDTVTFVVNRNINVSNICTVGCAFCGFGQGRRSPDAYEHDRDE
                     FVARVNQAVAYGATELCMQSGIHPDWGLEEYESWLRVAKETAPQLHLHAYSAMEVDHM
                     CTVSGLPPAEVFARLREAGLGSTPGTAAEVLDDGVRQRISPNKLPVDRWVEIIEASHR
                     TGLRSTVTVMFGHIEEPWELAEHMRVVRGLQARTGGFTEFVPLSFIPYHTMLGRTHGI
                     EEISIEENLKHTAAFRLALGKAVPSLQASWVKMGIEGATEALRWGVNDLGGTLMEESI
                     SRLAGSQHGVKLDPADLIGAAHRAGRPAAERTTLYELRQRHELPLAA"
BASE COUNT          470 a         1315 c         1286 g          530 t
ORIGIN      
        1 cctggcccgg gccaaggacg gcggcacgcc cgccgtcctg gtgcgcggcc tggaacggtt
       61 cgtcaccgag aacgacgggc cgggcgccgc cgccctgcgc cgcccgcccg agctggacct
      121 gttccgctag gccctgcggc ggccgcggaa ggggcccgag acctcgtaga cctcgccctt
      181 gaggtcgttg gcgcgctggg aggagaagta gaaccggtcg ccggcggggt tgaaggcgat
      241 gcccgtgagc tcggagccca cgtggttgcc gccgctgacg gccaggaacc gcgatgcctt
      301 ccgccgcggc gtgatcagcc cgatgtggat ctcctcggtg gagatgtcct cgcacacgaa
      361 cagctcgccg gccctggacg ccgtcaggtt gtcgacccgg gtcagcggcg cgtcgcgcga
      421 ggccaggccg tcgtagatga cctcgatccg gccgcggcgg gtgtcgtagg cgtggatgcg
      481 cgagtcggcg gtggtcgaca ggtacacggt gccgctgtcg aaccagatgc cctcggcgcg
      541 gccgaactcc gtgatgccct tgacctggcg ccggatcggc gtccggcggg cgaaccggtc
      601 gggcacgtcg acccactcca cggcaccgcc gcgggcgacc ttggccatct ccagccggcc
      661 ctcggccagc gagggccacc gccgcggcgt gtagcggtag aacgcgccgt cgatcagatc
      721 ctcggtcatg tacacgtgcc gcccgcgcgg gtccacggcc gcggcctcgt gcttgaacac
      781 gcccatcgcg tcgtgcacgc gcgccttgct gcgcccggtg gggtcgcact cccacacccg
      841 gccttcctcg aactcctcgc acgagagcca ggtggcccac ggcgtcgggc cgccggcgca
      901 gttctgggtc gtcccgtcca ggatccggta ggcatcctcg atctggcccc gggggccgaa
      961 gcgcatggcc cccgccccac cgcgctgctc ctcggagttg gacaccagga tccagccgcc
     1021 atccctggtc gggaaggtgg ccgacccgtc ggaggccttg tgccagtcgt agccggtgcc
     1081 gggcacgcgc tcggttccgc gggccacgcg ccggacggtg aacccgtccg gcacggcgac
     1141 gccgtcggcg ttgggcctgc cgtaggtgcc gtaggcctgg gccggtggcc gcgatgtggc
     1201 ttcgccgccg gccgcgacct ccttccagaa caccgcgccc agggccagcg tcgccgccgc
     1261 gcccacgaac tcccgcctgc ggtacaccgg ctcagtcacc gggccccagg ctacgagcgg
     1321 ccgtgggtgc ggacgtaaac gacctgtgac tacgcggcca gcggcagctc gtggcgctgc
     1381 cggagctcgt agagcgtggt ccgctccgcc gccgggcgcc ccgcgcggtg ggccgcgccg
     1441 atcaggtcgg ccgggtcgag cttgaccccg tgctggctgc cggccaggcg cgagatcgac
     1501 tcctccatca gcgtgccgcc caggtcgttc acgccccagc gcagcgcctc ggtcgccccc
     1561 tcgatgccca tcttcaccca cgaggcctgg agcgagggca cggccttgcc cagcgccagc
     1621 cggaacgcgg cggtgtgctt gaggttctcc tcgatcgaga tctcctcgat gccgtgcgtg
     1681 cggcccagca tggtgtggta cgggatgaag ctcagcggca cgaactcggt gaagccgccg
     1741 gtgcgggcct gcaggccgcg gaccacgcgc atgtgctcgg ccagctccca cggctcctcg
     1801 atgtggccga acatcacggt cacggtcgag cgcagcccgg tccggtggct ggcctcgatg
     1861 atctccaccc agcggtccac cggcagcttg ttgggcgaga tccgctggcg cacgccgtcg
     1921 tccagcacct cggccgccgt gccgggcgtg gagcccaggc cggcctcgcg cagccgcgcg
     1981 aacacctcgg ccggtggcag cccgctgacc gtgcacatgt gatcgacctc catcgccgaa
     2041 taggcgtgca ggtgcagctg cggcgcggtc tccttggcca cgcgcagcca ggactcgtac
     2101 tcctccaggc cccagtccgg gtggatgccg gactgcatgc acagctcggt ggcgccgtag
     2161 gccacggcct ggttcacgcg cgcgacgaac tcgtcgcggt cgtgctcgta cgcgtcgggc
     2221 gagcgccggc cctggccgaa gccgcagaac gcgcagccca ccgtgcagat gttggacacg
     2281 ttgatgttgc ggttgaccac gaacgtgacc gtgtcgccgg ccagctcggc ccgcagctcg
     2341 tcggccgcct gacgcatgtc ctcgatgacc tcgggccgcg tctcggcgaa cagcgcggtc
     2401 agctcctcgg gctccagctc ctcgccgcgg cgggcgcgct cgatggcgcc cgggctgagc
     2461 tcggagtgga tcgggcggtc ctcgcgccga cccgagccct tgcgcgggat gaagctccag
     2521 aacgaggtct tgaccacgtc caggacgccc tgctccatcc actcggagtc catgtactgc
     2581 gggtacacgc acagccgctc ggtcagcgcg tagccgcgcg gctggaggtc cttgcgcatc
     2641 cggtgcggcg tggggaacgg gtgctcgggc gagatgtggt cgccgttggc cgacaggccg
     2701 cccaggtcgg tggcgccctc ctccaccagc ggcagccacc actcggccag gttgggcggg
     2761 atctgcacgc cgacgtcggg catcagctcc agcgaggcgc gcaccagcgc gcgcatctcg
     2821 tccaggccca cgtcctgacc ccaggagggc atcggcgccg ctgtacccgc ggtctgcgaa
     2881 cgctgggcgt cgtcggcgat cagcgccggc tcggcgccgt agtagcgcgg gtggggcacg
     2941 aagttctgca ggatcacctc ctggaggtgg ccgtgctcgg cgtgcagggc ggccagggcc
     3001 tccagcgcct cgatccgctg ggccggcgtc tcgccgatgc ccaccaggat cccgctggtg
     3061 aaggggatct tcagctcgcc cgcgtcacgg atggtctgca ggcggacgtc ggggtgcttg
     3121 gtcggcgagc cctggtggac caccaggtcg gggttcaccg actccagcat caggccctgg
     3181 gacgccgtga cctcgcgcag ccgcgccagg tcgcgcctgg acaggacgcc gaggttggtg
     3241 tgcgggagca tcccccgctc cagcgcgcgc tcacaggtcc aggccacgta ggaggtgaag
     3301 tcctcgtggc cgtaggactc cagccgccgc gccacctcgg ggttgacctc gggcttctcg
     3361 cccgtgagca ccagcagctc cttggcgttg cgcttgaccg cctcgtccag cagtccctcg
     3421 acctcgtcgg ggtcgtacag gtgcgcctgg tgggtggcga acgcgcagta cttgcagtaa
     3481 caccggcacg tgcgcgacag cgacaccgtg tagttgcggc tgaacgtgac gcggcgcaca
     3541 gccttcgatc ctagacaggg gcgcggagga gccgcggccg cgcttagcat gggctctcgt
     3601 g
//
LOCUS       DASUPW010000072         5099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_569171, whole genome shotgun sequence.
ACCESSION   DASUPW010000072 DASUPW010000000
VERSION     DASUPW010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569171"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..846)
                     /locus_tag="VFY44_01670"
     CDS             complement(136..846)
                     /locus_tag="VFY44_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909168.1"
                     /translation="MVAAAASLAVVAGCGSDPERRAATAPDDSVVLGTDPVPTPTETA
                     RKPAKPKPKAEPEARPQAPDPTTAAREPGRVTVIGDSLAVGTQTLLPAALPGWSVSTD
                     ARKGRPLAEGMQVLRGADVPAGSVLVFSLFTNDTPSATGALEAALRAAAEKVGPQGCQ
                     VWATIARPVPGGGGEPANRILRDLDGGQVSGTKVVVADWADLVSQRPALLAPDGVHGT
                     PEGYRLRARLYADAVRRC"
     gene            complement(858..2078)
                     /locus_tag="VFY44_01675"
     CDS             complement(858..2078)
                     /locus_tag="VFY44_01675"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX5909169.1"
                     /translation="MRTGPGVLTFVVALACLAAAPATAQAPVQLSASLEARGYIGISA
                     SAPEGGPLLVGEFWRGTFEPITQSPTGSVSVRRALTWRCDRRHRLLVAKSLAGGRLSA
                     PVRVRTPSCRKRFKVVLKPSRPRAGRPLSVLVLDRFGSGDVTLRVCAEPPRARARCRD
                     LRLARGRGRGAVRFKASAGRWPVTVSTRGAPRARRVAVVRRRGGGLRILATGDSMIQI
                     LDSFLQQRLQRGRVRVRKESHISTGISKPSMLNWNTKARRQARGIRPDASVVFLGAND
                     GFPIGRSRCCGRGWVRGYTRRVRDMMRSYSRGGSGTVYWLTLPVPRKAAFKPIYRAVN
                     ASIRRAARAYPDTVRVVDAEAVFTPGRRYTSTIRFGGRNVNVRQDDGVHLNTAGASIA
                     ATLVIRAMRRDGVL"
     gene            complement(2113..3147)
                     /locus_tag="VFY44_01680"
     CDS             complement(2113..3147)
                     /locus_tag="VFY44_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX5909170.1"
                     /translation="MAREPQDSVPEAPRGRRLPARDSVLVVVGALLILILLAGGSVRS
                     AGEEMDPGFQRDVVLAVGKPTGWLADRLPLESAAGDALAWLSPDDDLDGGGGFAEAAT
                     PAGGGGRRAAAGGPVSPSEFDPVELGAKAERPRELKTVLVTGDSLAQPRDADLARRLA
                     GDDVRTVRDVHVGTGISKTDLLDWGKLSAKQVAKDEPDAVVVFIGANEGFPMPGARGR
                     TVQCCGPGWAAAYATRVRQMMRTYRQDGSARVYWLTLPFPREKSRQEIARAVNAAIEV
                     AAGPFRAQVRPLDMVPVFTPEGRYRDAVETDGRKRVVRDPDGIHLNGEGASVAADEVE
                     QAIQKDFGSP"
     gene            complement(3148..4542)
                     /locus_tag="VFY44_01685"
     CDS             complement(3148..4542)
                     /locus_tag="VFY44_01685"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEX5909171.1"
                     /translation="MVFPSIQFAIFLPLVLAISWALMPRPKLWKPFILAASYLFYAAA
                     SPRYCLLLAAVTLANQLGAVAVHRARDERRQKAIIAATVGFDLAVLGVFKYYGFFANE
                     IGAFLDDIGLPMPLPLLTLALPIGLSFITFQAISYSVDVKRGLLAPASTLDFAIYLSF
                     FPHVVAGPIVRAREFIPQLSTPRDPRNVAVGAGVVLVMLGLIKKVAIADLLAREVVDP
                     VFAVPQAYAAPDVLLAAYAYAAQIYCDFSGYTDIAIGVALLAGFVFPQNFRSPYRSIS
                     FREFWQRWHITLSRFLRDFLYIPLGGNRGGRWKTARNLMLTMVLGGLWHGAAWGFVLW
                     GTVHGLALVTEHQLRGRVKAPPVWLRWAIVFHVVVLAWVLFRAETLTLAGEFFSRLVV
                     PGDATLFTPAVVAAVVAVIGFQLLPERPLERLRERIADFRPVALAASLALVVVVVGST
                     VPSQGVPPFIYFQF"
     gene            complement(4554..>5099)
                     /locus_tag="VFY44_01690"
     CDS             complement(4554..>5099)
                     /locus_tag="VFY44_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909172.1"
                     /translation="RVVARVRPGTALFKPGRPSWLQTARAQSSTLRPAATVVSLGIND
                     GYPIRTADGQRLRCCSERWLDLYTRRVRTMMRSWTRYGGQVAWMTLPPTAVPELVPLL
                     AAVNTAVLRASVGLPRVSVVRVDRLVSPDGRYTPTKVVGGRRVKLYLSDRLHLAPAGT
                     AIATRAVVAALRRNGGLSHRR"
BASE COUNT          715 a         1961 c         1842 g          581 t
ORIGIN      
        1 ccagccgcgc cgcgtgcacg cgggcgtgct gcgccgtggc gaccgtggcg tcggcgcgag
       61 gggtcgtcac gcgcagccgg gcctgcgccg gggccgccac cgccagcgcc gccagcgcgg
      121 tagccgcgac cgtcctcagc accggcggac cgcgtccgcg taaagccgcg cgcgcagccg
      181 gtagccctcc ggcgtcccgt gcaccccgtc gggggccagc agcgcgggcc gctgggacac
      241 cagatcggcc cagtcggcca cgaccacctt cgtcccggac acctggcccc cgtccaggtc
      301 ccgcaggatc cggttggccg gttcgccccc gccgcccggc acgggcctgg cgatcgtggc
      361 ccagacctgg cagccctggg gccccacctt ctccgcggcg gcgcgcagcg cggcctccag
      421 cgcgccggtg gccgagggcg tgtcgttggt gaacaggctg aacaccagca ccgagcccgc
      481 gggcacgtcg gccccgcgca gcacctgcat cccctcggcc agcggcctgc ccttgcgcgc
      541 gtcggtgctg accgaccagc cgggcagggc cgcggggagc agggtctgcg tgcccaccgc
      601 cagcgagtcg ccgatcacgg tcacccggcc gggctcgcgc gcggcggtgg tggggtcggg
      661 cgcctgcggc cgggcctcgg gctcggcctt cggcttcggc ttcgccggct tgcgcgcggt
      721 ctcggtcggg gtggggacgg gatcggtgcc cagcacgacg ctgtcgtcgg gggccgtggc
      781 cgcgcggcgc tcggggtcgg agccgcagcc ggccaccacc gccaggctcg ccgccgccgc
      841 gaccatcgcc acgtgcctca cagcacgccg tcgcggcgca tggcgcggat caccagtgtc
      901 gcggcgatcg acgcccccgc ggtgttcagg tgcacgccgt cgtcctggcg cacgttcacg
      961 tttcggccgc cgaacctgat cgtcgatgta taccgccggc ccggggtgaa caccgcctcg
     1021 gcgtccacga cccgcaccgt gtcggggtag gcgcgcgcgg cgcggcggat ggacgcgttc
     1081 acggcgcggt agatcggctt gaaggcggcc ttgcgcggca ccggcagcgt cagccagtac
     1141 accgtgccgg agcccccgcg cgagtagctg cgcatcatgt cgcgcacccg ccgggtgtag
     1201 ccccggaccc agccccgccc gcagcaccgg gagcggccga tcgggaagcc gtcgttggcg
     1261 cccaggaaca ccacgctggc gtcggggcgg atgccgcggg cctgcctgcg ggccttggtg
     1321 ttccagttca gcatcgaggg cttggagatc ccggtgctga tgtgcgactc cttgcgcacg
     1381 cggacccgcc cgcgctgcag gcgctgctgg aggaagctgt ccaggatctg gatcatcgag
     1441 tcgccggtgg ccaggatccg caggccgccg ccgcgccggc gcacgaccgc cacccggcgc
     1501 gcacgcgggg cgccgcgcgt gctcacggtc accggccacc gcccggccga ggccttgaag
     1561 cgcaccgcgc cccgtccccg gccccgggcc agccgcaggt cacggcagcg ggcgcgtgcc
     1621 cgcggcggct cggcgcagac cctcagcgtc acgtccccgg agccgaagcg gtccagcacc
     1681 agcaccgaca gcggccggcc ggcgcgcggg cgcgacggct tcagcacgac cttaaatcgc
     1741 ttgcggcagg acggcgtccg aacgcgcacc ggcgcggaca gcctgccacc ggccagtgac
     1801 ttggccacca gcagccggtg gcggcggtcg caccgccacg tcagcgcgcg ccgcaccgac
     1861 accgagcccg tgggcgactg cgtgatgggc tcgaaggtcc cgcgccagaa ctcgccgacc
     1921 aggagcgggc cgccctcggg ggcggaggcg ctgatcccga tgtagccacg ggcctcgagc
     1981 gaggcggaca gctgcaccgg cgcctgggcg gttgccggcg ccgcggccag gcacgcgagc
     2041 gcgacgacga atgtgagcac ccccgggccc gtccgcatcg cgcggaccat acccagcggc
     2101 gtgacgcgat tgctagggcg agccgaagtc cttctggatc gcctgctcga cttcgtccgc
     2161 ggccaccgat gcgccctcgc cgttcaggtg gatgccgtcg gggtcgcgga ccacgcgctt
     2221 gcgcccgtcg gtctcgacgg cgtcccggta gcggccctcg ggcgtgaaca ccggcaccat
     2281 gtccagcggc cgaacctggg cccggaacgg cccggccgcc acctcgatcg cggcgttcac
     2341 ggcacgcgcg atctcctggc gggacttctc gcgcgggaag ggcagcgtca gccagtacac
     2401 ccgcgcggag ccgtcctggc ggtaggtccg catcatctgg cgcacgcgcg tggcgtaggc
     2461 cgccgcccag ccggggccgc agcactgcac cgtgcgcccg cgcgcgcccg gcatcgggaa
     2521 gccctcgttg gccccgatga acaccaccac cgcgtcgggc tcgtccttgg ccacctgctt
     2581 ggccgacagc ttgccccagt ccaggaggtc ggtcttcgag atcccggtcc ccacgtgcac
     2641 gtcgcgcacg gtcctgacgt cgtctccggc cagccgccgc gccagatcgg cgtcccgggg
     2701 ctgggccagc gagtcgccgg tgaccagcac ggtcttcagc tcccgcgggc gctcggcctt
     2761 cgcgcccagc tccacggggt cgaactccga gggcgacacc ggcccgccgg ccgcggcccg
     2821 cctaccgcca ccaccggccg gggtggccgc ctcggcgaag ccgccgccgc cgtccaggtc
     2881 gtcgtcgggc gagagccacg ccagcgcgtc gccggccgcc gactccagcg ggaggcggtc
     2941 ggccagccag cccgtgggct tgcccaccgc cagcaccacg tcacgctgga acccggggtc
     3001 catctcctcg ccggcgctgc gcaccgagcc cccggccagc aggatcagga tcagcagggc
     3061 gccgaccacc accagcaccg agtcgcgcgc cggcaggcgg cggccacgcg gggcctcggg
     3121 cacgctgtcc tggggctcgc gggccatcta gaactgaaaa tagatgaacg ggggcacgcc
     3181 ctggctgggc accgtggagc cgaccaccac gaccaccagc gccaggctgg ccgccagcgc
     3241 caccgggcgg aagtccgcga tgcgctcgcg caggcgctcc agcgggcgct cgggcagcag
     3301 ttggaagccg atcaccgcga ccaccgccgc caccaccgcg ggggtgaaca gcgtggcgtc
     3361 gccgggtacg accaggcgcg agaagaactc gccggcgagc gtcagcgtct cggccctgaa
     3421 cagcacccag gccagcacca cgacgtggaa cacgatcgcc caccgcagcc acaccggcgg
     3481 cgccttcacg cggccgcgga gctggtgctc ggtgaccagc gccagcccgt gcaccgtccc
     3541 ccacaggacg aatccccacg cggcgccgtg ccacaggccg cccaggacca tggtcagcat
     3601 caggttgcgc gccgtcttcc agcgcccacc gcggttgccg cccagcggga tgtacaggaa
     3661 gtcgcgcagg aaccgcgaca gcgtgatgtg ccaccgctgc cagaactcgc ggaagctgat
     3721 cgaccggtac gggctgcgga agttctgggg gaacacgaag cccgccagca gggccacccc
     3781 gatggcgatg tcggtgtagc ccgagaagtc gcagtagatc tgggccgcgt aggcgtaggc
     3841 ggccaggagc acgtcgggcg ccgcgtacgc ctgcgggacg gcgaacaccg ggtccaccac
     3901 ctcgcgcgcc agcaggtcgg cgatggccac cttcttgatc agccccagca tgaccagcac
     3961 cacgcccgcc cccaccgcca cgttgcgggg gtcgcgtggg gtggacagct gcgggatgaa
     4021 ctcccgcgcg cgcacgatcg ggccggccac cacgtgcggg aagaagctca ggtagatggc
     4081 gaagtccagc gtgctggccg gcgccagcag cccgcgcttg acgtccaccg agtacgagat
     4141 ggcctggaag gtgatgaagc tcaggccgat cggcagcgcc agcgtcagca gcggcagcgg
     4201 catcggcagc ccgatgtcgt ccaggaaggc gccgatctcg ttggcgaaga agccgtagta
     4261 cttgaacacg cccagcaccg ccaggtcgaa gcccacggtg gcggcgatga tggccttctg
     4321 acgccgctcg tcgcgcgcgc ggtgcaccgc cacggcgccg agctggttcg ccagcgtcac
     4381 ggccgccagc agcaggcagt agcgcgggct ggccgccgcg tagaacaggt agctggccgc
     4441 caggatgaac ggcttccaca gcttcggccg cggcatcagg gcccacgaga tggccagcac
     4501 cagcggcagg aagatggcga actggatgga ggggaagacc acggcaggcc gtctcagcgc
     4561 cggtgggaga ggccgccgtt acggcgcagc gcggccacga ccgccctggt ggcgatcgcc
     4621 gtgcccgcgg gcgccaggtg caggcggtcg ctcaggtaca gcttcacgcg ccgtcccccg
     4681 acgaccttgg tcggcgtgta gcgcccgtcc gggctgacca gccggtcgac acgcaccacc
     4741 gagacccgcg gcagccccac ggacgcgcgc aacacggcgg tgttcacggc cgccagcagc
     4801 gggaccagct ccgggaccgc ggtcggcggc agggtcatcc aggccacctg gccgccgtag
     4861 cgcgtccagc tccgcatcat cgtccggacg cgtcgcgtgt acaggtccag ccaccgctcg
     4921 ctgcagcagc gcagccgctg cccgtcggcc gtgcggatcg ggtagccgtc gttgatgccc
     4981 agcgacacca cggtcgcggc gggcctgagc gtggaggact gcgcccgcgc cgtctgcagc
     5041 cagctcggcc ggccgggctt gaacagcgcc gtgccggggc gcacccgggc caccacgcg
//
LOCUS       DASUPW010000073         5375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1954918, whole genome shotgun sequence.
ACCESSION   DASUPW010000073 DASUPW010000000
VERSION     DASUPW010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1954918"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VFY44_01695"
     CDS             complement(<1..312)
                     /locus_tag="VFY44_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HEX5909173.1"
                     /translation="MRKALLALILAVLAALAVASAPVAGAQAATKSPRLKAGVGKADI
                     TPQTGYYLGGWTRADRIAQGQHTRLFSRAMVLERQGRKFALVQVDLFMVPGGMVKQIG
                     EI"
     gene            584..988
                     /locus_tag="VFY44_01700"
     CDS             584..988
                     /locus_tag="VFY44_01700"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5909174.1"
                     /translation="MARHRTNLTHRKPGTPGFWRHPYYDEWGEVVGGRIRRLRQERGW
                     TLIDLAAEVPKPEGGWYSGGFFSRLERGWTSAPFYVYVHAARALGARPGELLGLDDVQ
                     KPLSPGEDALLRFVDRLGIEPAEAMARLAEQR"
     gene            complement(985..2061)
                     /locus_tag="VFY44_01705"
     CDS             complement(985..2061)
                     /locus_tag="VFY44_01705"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR01208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HEX5909175.1"
                     /translation="MGSIKGLILSGGKGTRLRPLTHTSAKQLVPVANQPVLFYGIRAM
                     AEAGIEEVGIIVAPETGGEIREAAGDGSQFGVSITYIDQAEPLGLAHAVLTAEPFIGD
                     QPFVMYLGDNLLRNGITDLVHTFEADSPDALILLTKVPDPENYGVAELAEDGRVSRLV
                     EKPAEPATDLALVGVYMFTSGIFDAAKAIEPSPRGELEITDAIQYLVDGGKRVDPHVV
                     QGWWKDTGQVADMLEANRLILDAMVERLDGQITDCQIEGRVVVEAGATLERATVRGPA
                     VIGAGAVIRDAYIGPYTAIAEDVTIEHAELEHSIVLSGSTIRDLDARIEASLIGKNAT
                     VARSPTVPKAHRFVIGDNSEVHIL"
     gene            complement(2048..2518)
                     /locus_tag="VFY44_01710"
     CDS             complement(2048..2518)
                     /locus_tag="VFY44_01710"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5909176.1"
                     /translation="MASRRDQIRMTDEELAAFLDEQRVVQVCTTGPSGRPHLMPLWYV
                     VQDGPVLRGWTFAKSQKAKNLERDPRATLGIDDGVQYHELRGVMFECDVELERDPERL
                     AELGESIFKRYGDGSDLPEEAREMVRKQAEKRVGLTFTPTRTVTWDHRKLGGVY"
     gene            complement(2543..4078)
                     /locus_tag="VFY44_01715"
     CDS             complement(2543..4078)
                     /locus_tag="VFY44_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909177.1"
                     /translation="MGTRRRLILIAVLGAALGGGSDAWAAEGVKGQKGTEETLLVGNN
                     WDGTADVVDPVRFKRITRINIVPDLAIRRAEIMADPVGLGFYLGVRQLIGEGNDQLVD
                     DMFTSPDGRFLYVSRPSLKDVVAFNLRTKQIHWRVPVEGYRSDHMAISPDGKRLLVSA
                     STASKVHVIDTATGRIAANFESGDQPHESNYSRDGTTIFHASIGTVYTPTDDPSQDAT
                     KGKRYFQIVDAKDYRVLRRVEMGKKLAEAGFPNMSSAVRPMAITPDERFAYLQISFFH
                     GFAEYDVRQDKVTRLARLPLSEKAKGLRRDEYVLDSAHHGLSINPSGTKLCAAGTMSD
                     YAAIVDRSDFAYKVVPVGDKPYWSTNSGDGRNCFVSVSGEDRVSVISYAGAREIASIP
                     VGDHPQRMRLGPIRSEFLPGDRVAPATSRGRVVRSRRSGRRSLRVRLSESARLTVDVR
                     RVKGRKTRRVRVLRRTVAKGTRTIGLGSLAKGRYRIVFRARDAAGNASPRGTVRYRAR
                     RAG"
     gene            complement(4104..>5375)
                     /locus_tag="VFY44_01720"
     CDS             complement(4104..>5375)
                     /locus_tag="VFY44_01720"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX5909178.1"
                     /translation="QQDVAKVWLSDRIDWLVRRHLGPDVGTLEDTDDGAVFTTAYGDS
                     RQLISWVLGLGPRARILEPPELVEEAEERVAQVVERHSEPVELADPAPRRARETDEDD
                     GKRESTNRPERFARLVTLAGILIDAARRGGKLEMRELTESLQISEAELRQDIDVLNVV
                     NFGGGSYVLYAEIQGDTIEVDPEPYGDNFARPARLLPLEAKALVAAIDLVGEHLPEGS
                     LASARDKIVDALGRDPATDGLQITTAKGDDSEIARVVSGAIQDRRLLEIEHYKEDEDE
                     FTSRTIEPYQLMNGAEGWYVHAFEPAKDATRSFRLDRIRSANELDETYEPREGVEPDV
                     AGWPTTGEVPTSEHARVWVSPDRARWAREDRSVIQELKDGAVIVELAFGGHDYLAREI
                     LKEAGDAVVLEPEDARDAVRSAAEALLPARR"
BASE COUNT          702 a         1923 c         1930 g          820 t
ORIGIN      
        1 gatctcgccg atctgcttga ccatgccgcc cgggaccatg aacaggtcca cctgcaccag
       61 ggcgaacttg cgcccctggc gctccagcac catggcccgc gagaacagcc gcgtgtgctg
      121 gccctgcgcg atccggtccg cccgcgtcca gccgcccagg tagtagcccg tctgcggcgt
      181 gatgtccgcc ttgcccacgc cggccttcag gcgcggagac ttggtcgcgg cctgggcccc
      241 ggccaccggc gccgaggcca cggccagcgc cgccagcacg gccaggatca gggcgaggag
      301 ggccttccgc acccgaccaa tctacccccg ggcctcccgt caggcgccgg tagtgggacg
      361 gctgtcgccc ctgaacacac ggtcggatct gtggcaacgc ggttgtcccg gaggcagggc
      421 cggtgccggg acgtcaagaa ggcttgccgg tagcgcacgt ccgttaccgg aggagcaccg
      481 agcgtgacga cagcgaaacg ttctgtacgg agatccgagc gggtgttccg gcactgcatc
      541 gagatcccca cgcaggcccc ggcgcggcct aggttcgccc ggcatggccc gccatcgcac
      601 gaacctcacc caccgcaagc cgggaacccc cggcttctgg cgacacccct actacgacga
      661 atggggcgag gtcgtgggcg gccgcatccg gcgcctacgc caggagcgcg gctggacgct
      721 gatcgacctg gcggcggagg tgcccaagcc cgagggcggg tggtactcgg gcggcttctt
      781 ctcgcgcctc gagcgcggct ggacgagcgc gccgttctac gtctacgtcc acgcggctcg
      841 cgcgcttggc gccaggccgg gcgagctgct gggcctggat gacgtgcaga agccgctctc
      901 ccccggggag gacgcgctgc tgcgcttcgt ggaccggctg ggtatcgagc ccgccgaggc
      961 gatggcccgg ctggccgagc agcgctagag gatgtggacc tcggagttgt ccccgatcac
     1021 gaagcggtgc gccttgggca cggtgggtga gcgggcgacg gtcgcgttct tgccgatcag
     1081 ggaggcctcg atgcgggcgt ccaggtcgcg gatggtcgag cccgagagga cgatcgagtg
     1141 ctcgagctcg gcgtgctcga tggtcacgtc ctcggcgatc gcggtgtagg ggccgatgta
     1201 ggcgtcgcgg atgaccgcgc cggcgccgat cacggccggc ccgcggacgg tggcgcgctc
     1261 gagggtggcg cccgcctcga ccacgacgcg gccctcgatc tggcagtcgg tgatctggcc
     1321 gtcgaggcgc tcgaccatgg cgtcgaggat caggcggttg gcctcgagca tgtcggcgac
     1381 ctggccggtg tccttccacc agccctggac cacgtgcggg tccacgcgct tgccgccgtc
     1441 gaccaggtac tggatcgcgt cggtgatctc cagctcgccg cggggcgagg gctcgatcgc
     1501 cttggcggcg tcgaagatgc cgctggtgaa catgtacacg cccaccaggg ccaggtcggt
     1561 cgcgggctcg gccggcttct cgacgagcct ggacacgcgc ccgtcctcgg ccagctcggc
     1621 cacgccgtag ttctcgggat ccgggacctt ggtcagcagg atcagcgcgt ccgggctgtc
     1681 ggcctcgaac gtgtgcacca ggtcggtgat gccgttgcgc agcaggttgt cgcccaggta
     1741 catcacgaac ggctggtcgc cgatgaacgg ctcggcggtc agcacggcgt gggccaggcc
     1801 cagcggctcg gcctggtcga tgtaggtgat cgacacgccg aactgggagc cgtcgccggc
     1861 cgcctcacgg atctcgccgc ccgtctccgg ggcgacgatg atgcccacct cctcgatgcc
     1921 ggcctcggcc atcgcccgga tgccgtagaa cagcaccggc tggttggcca ccggcaccag
     1981 ctgcttcgcc gacgtatgcg tgaggggccg gagccgcgtg cccttgcccc ccgacaggat
     2041 caggccctta atagacccca cccagcttcc ggtggtccca cgtcacggtc cgggtggggg
     2101 tgaaggtcag ccccacgcgc ttctcggcct gcttgcgcac catctcgcgc gcctcctccg
     2161 gcaggtccga gccgtcgccg tagcgcttga agatgctctc gcccagctcg gccaggcgct
     2221 cggggtcgcg ctccagctcg acgtcgcact cgaacatcac gccgcgcagc tcgtggtact
     2281 gcacgccgtc gtcgatgccc agcgtggcgc gcgggtcgcg ctccaggttc ttggccttct
     2341 gcgacttggc gaacgtccag ccgcgcagca ccgggccgtc ctggaccacg taccagagcg
     2401 gcatcaggtg gggccgtccc gagggtccgg tggtgcacac ctgcacgacg cgctgctcgt
     2461 ccaggaacgc ggcgagctcc tcgtcggtca tgcggatctg gtcgcgccgg gaggccatcg
     2521 cgcgcagcct acgcgttcgc ggtcagccgg cgcggcgtgc gcggtagcgg acggtcccgc
     2581 gcggggaggc gttgcccgcg gcgtcgcggg cgcggaagac gatccggtag cggcccttgg
     2641 ccagcgagcc gagcccgatc gtccgcgtcc ccttggccac cgtacgccgc agcacccgca
     2701 cccggcgcgt cttgcgcccc ttcaccctgc ggacgtccac ggtcagccgc gccgactcgg
     2761 acagccgcac ccgcagggag cggcggccgc tgcgacggct gcgcaccacg cgcccgcgtg
     2821 acgtcgcggg cgccacccgg tcaccgggca ggaactcgga ccggatcggc cccaggcgca
     2881 tgcgctgcgg gtggtcgccg acggggatgc tggcgatctc gcgggcgccg gcgtaggaga
     2941 tgaccgacac gcggtcctcg ccgctgaccg agacgaagca gttgcgcccg tcgccgctgt
     3001 tggtggacca gtagggcttg tcgcccacgg gcacgacctt gtaggcgaag tcggagcggt
     3061 cgacgatcgc cgcgtagtcg gacatcgtcc cggccgcgca cagcttggtg ccggacgggt
     3121 tgatcgacag cccgtggtgg gcggagtcca ggacgtactc gtcgcggcgc aggcccttcg
     3181 ccttctcgct gagcggcagc cgggccaggc gcgtgacctt gtcctggcgg acgtcgtact
     3241 cggcgaaccc gtggaagaag gagatctgca ggtaggcgaa ccgctcgtcg ggggtgatgg
     3301 ccatcgggcg caccgccgag ctcatgttgg ggaacccggc ctcggccagc ttcttgccca
     3361 tctccacccg cctgagcacg cggtagtcct tggcgtcgac gatctggaag tagcgcttgc
     3421 ccttcgtggc gtcctgggac gggtcgtcgg tgggtgtgta gaccgtgccg atgctggcgt
     3481 ggaagatcgt ggtcccgtcg cgcgagtagt tgctctcgtg cggctggtcg cccgactcga
     3541 agttggcggc gatgcggccg gtcgcggtgt cgatcacgtg caccttgctc gcggtcgacg
     3601 ccgacaccag cagccgcttg ccgtcgggtg agatggccat gtggtccgag cggtagccct
     3661 cgaccgggac gcgccagtgg atctgcttgg tgcgcaggtt gaacgcgacg acgtccttca
     3721 ggctcggccg cgagacgtac aggaagcgcc cgtcgggcga ggtgaacatg tcgtcgacca
     3781 gctggtcgtt gccctcgccg atcagctggc gcacgccgag gtagaacccg aggcccaccg
     3841 ggtcggccat gatctcggcc cgcctgatcg ccaggtcggg aacgatgttg atgcgcgtga
     3901 tgcgcttgaa gcggaccggg tcgaccacgt cggccgtgcc gtcccagttg ttgcccacca
     3961 gcagggtctc ctcggtgccc ttctggccct tgaccccctc ggccgcccac gcgtccgagc
     4021 cgccgccgag ggccgcgccc agaactgcga tcaggatcag acggcgccgg gtgcccacgc
     4081 cggcgatcgt acccccgccg ggctcagcgg cgcgccggca gcagcgcctc ggctgccgac
     4141 cgtacggcgt cgcgcgcgtc ctcgggctcg agcaccacgg cgtcgccggc ttccttcagg
     4201 atctcgcgcg ccaggtagtc gtggccgccg aaggccagct ccacgatcac ggcgccgtcc
     4261 ttgagctcct ggatcaccga gcggtcctcg cgcgcccagc gggcccggtc gggggacacc
     4321 cacacccgcg cgtgctcgga ggtggggacc tcgccggtgg tgggccagcc ggccacgtcg
     4381 ggctcgaccc cctcgcgcgg ctcgtaggtc tcgtccagct cgttggccga gcggatgcgg
     4441 tccaggcgga aggagcgcgt ggcgtccttg gccggctcga aggcgtgcac gtaccagccc
     4501 tcggccccgt tcatcagctg gtagggctcg atcgtccggg acgtgaactc gtcctcgtcc
     4561 tccttgtagt gctcgatctc cagcagccgc cgatcctgga tggcgcccga gaccacgcgc
     4621 gcgatctcgg agtcgtcgcc cttggcggtg gtgatctgca gcccgtccgt ggccgggtcg
     4681 cggcccagcg cgtcgacgat cttgtcgcgg gccgaggcca gcgacccctc cggcaggtgc
     4741 tcgccgacca ggtcgatggc ggcgaccagc gccttcgcct ccagcggcag cagccgggcg
     4801 gggcgggcga agttgtcgcc gtagggctcg gggtcgacct cgatcgtgtc gccctggatc
     4861 tcggcgtaga gcacgtacga gccgccgccg aagttgacga cgttcagcac gtcgatgtcc
     4921 tggcgcagct cggcctcgga gatctgcagg ctctcggtca gctcgcgcat ctcgagcttg
     4981 ccgccgcgcc gggccgcgtc gatcaggatg ccggccagcg tgaccagccg ggcgaagcgc
     5041 tcggggcggt tcgtgctctc gcgcttgccg tcgtcctcgt cggtctcacg cgcgcgccgc
     5101 ggcgcggggt cggccagctc caccggctcg gagtggcgct cgaccacctg ggccacgcgc
     5161 tcctccgcct cctcgacgag ctccggcggc tccaggatcc gcgcgcgcgg gcccaggccc
     5221 agcacccacg agatcagctg gcgcgagtcg ccgtaggcgg tggtgaacac cgcgccgtcg
     5281 tcggtgtcct ccagcgtgcc cacgtccggg ccgaggtggc ggcgcaccag ccagtcgatg
     5341 cggtcggaca gccacacctt ggccacgtcc tgctg
//
LOCUS       DASUPW010000074         4878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1816375, whole genome shotgun sequence.
ACCESSION   DASUPW010000074 DASUPW010000000
VERSION     DASUPW010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816375"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..608
                     /locus_tag="VFY44_01725"
     CDS             159..608
                     /locus_tag="VFY44_01725"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5909179.1"
                     /translation="MTDPTDAPQRRLRIAGLHHLTLLVSDLEASTAFYRNVLGLRMVK
                     QTVNEDDQGARHLFFGDEEGSPGTLITCLEYPQLEPGTVGVGSTHHFALSIETEEELH
                     AWADYLAAQGVAATEVMDRTFFKSLYVRDPDGHIVELATAGPGIVPD"
     gene            complement(634..930)
                     /locus_tag="VFY44_01730"
     CDS             complement(634..930)
                     /locus_tag="VFY44_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909180.1"
                     /translation="MARAEMKRDIAGGRLGVADVIVDCPWEVDTMSISDLLMSQKRWG
                     RARCRRLLTSIGIPENKPVGTLTDRQRSAVASVLRSKRPSAPRSESPFRALTSV"
     gene            complement(1224..1793)
                     /locus_tag="VFY44_01735"
     CDS             complement(1224..1793)
                     /locus_tag="VFY44_01735"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEX5909181.1"
                     /translation="MSHESESAKRAPDDLALVQRFVNTVELDEGPEDDELTDPDTLVA
                     WFAERGLCEPGARATQAELERAITVREGLRAVLDGHNGGEPAPDAVAALESAAGHASL
                     RASFTGERPELTPVCGGIDAGLARLLAAVTTAAADGTWQRLKACADEECRWAFYDHSR
                     NRSGRWCSMATCGNQQKARTYRERARADT"
     gene            1909..2148
                     /locus_tag="VFY44_01740"
     CDS             1909..2148
                     /locus_tag="VFY44_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909182.1"
                     /translation="MYSEITIRHATDNDVTAVRHLAALDESDAPKGDALLAFVDGELV
                     AARSTEGSVAVADPFRRTRHVMELLDLQRRQEQAA"
     gene            complement(2291..3538)
                     /locus_tag="VFY44_01745"
     CDS             complement(2291..3538)
                     /locus_tag="VFY44_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HEX5909183.1"
                     /translation="MDLFEKARSHDRLEQLKAAREHDLLPYFREVDGEPGPVVEMEGR
                     ERITLGSNNYLGLTTHPAVKQGALDALERYGTGLTGSRFLNGTTALHLELEREIADWT
                     GDEDALVFTTGYQANVGCVATLLGPEDTVIVDSGDHASILDAVMMSRARIRPFRHNKL
                     DKLEKMLDRSRSDGGGVLVVVDGVFSMEGDLAPLPEIVELCERYEARLMVDEAHGVGV
                     LGADGTGACEVFGVADRVDLRMGTFSKSLASCGGFISGPTEVIDFLRVQSRAFMFTAS
                     AVPAAVGATVAALRVIRSDEGRELLARVLDNARYLQRGLDERGFDVVQPTPMPDGSDL
                     LTPIVPVTIGDDWEAVLFWKALYDAGVYANVALYPAVPRGGALLRTSVMATHEHSHLD
                     KALEAFETARKAVEDSSGAPAPA"
     gene            3553..4683
                     /locus_tag="VFY44_01750"
     CDS             3553..4683
                     /locus_tag="VFY44_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909184.1"
                     /translation="MSIEVTPVRSLRDRREFIELPFRLHASSEQWVPPLRLERHAFLS
                     PRLNKWFRHADAQLFLARRGGRVVGRVSAQVDHAFNEYQGNDWGMFGFFEAEDDAEVA
                     TALLDAAAAWLTERGRDRMIGPVDFTMNDEAGLLIEGYDREPFIKQPWHPPYYRPLLE
                     GAGLEKAIDLWMWELHISDRSSIVQPIVDMAAQLEPEHGIRIRRMSRRRLRRELDVFA
                     ETYNEAWADNWGFVPYSSDDLDTYAGDLQLVFDPNWFMVAEKIDTGESVGVAITVPDI
                     NQVLKRMNGRLLPLGWWHFLRRRKTIDRVRVGFLGVKRDYQHTGVAAAFYMEHFDTAA
                     RTPQTWGEMGWILETNDAMNRGMEGMGGRVVKKYRMYERELP"
BASE COUNT          714 a         1728 c         1708 g          728 t
ORIGIN      
        1 gggcggcctc ggcctgcgcc tcaacccgct tggcctggtc ctgatcgcgg gcgcggtggc
       61 cttcgccgcg tcgttctggg gcctgcgccg gctcttccag aacaagtccg gcggcctgag
      121 cttccggtga cgcgggcgcg gcccgtacag tgctgcccat gaccgacccg accgacgccc
      181 cccagcggcg gctgcgcatc gccggcctgc accacctgac gctcctggtc tcggacctcg
      241 aggcctcgac ggccttctac cgcaacgtcc tgggcctgcg gatggtcaaa cagaccgtga
      301 acgaggacga ccagggcgcc cggcacctgt tcttcggcga cgaggaggga tcgccgggga
      361 cgctgatcac ctgcctcgag tacccgcagc tcgagcccgg cacggtcggg gtcgggtcga
      421 cccaccactt cgcgctctcg atcgagaccg aggaggagct gcacgcctgg gccgattacc
      481 tggccgccca gggagtggcc gcgaccgagg tcatggaccg cacgttcttc aagtcgctgt
      541 acgtgcgcga cccggacggc cacatcgtgg agctggccac cgccggcccg ggcatcgtcc
      601 cggactgacg gcggccgctc cggagagcgt cgtctagacg ctggtgaggg cgcggaaggg
      661 cgattcggag cgcggcgcgc tcgggcgctt ggagcgcagc acggaggcca cggccgagcg
      721 ctggcggtcg gtcagcgtgc cgaccggttt gttctcgggg atcccgatgg aggtcagcag
      781 gcggcggcag cgagcccggc cccaccgctt ctggctcatc aggaggtcgc tgatcgacat
      841 cgtgtcgacc tcccacgggc agtcgacgat cacgtcggcc acgcccaggc ggccccccgc
      901 gatgtcgcgc ttcatctcgg cgcgggccaa gcgcactcgg ttggcgtgct ccagtgccct
      961 caggtgctgc tgcccagcgt caatcgcggc ggtttcgccc atacgtaatc cctcccgcac
     1021 ttcgtgcaga tcccaggttt gccctacaga aagaggccct gcgctaccga cctccccggc
     1081 tccctctggc cggtgaggcc gtgtccgcgg ccggggacca ataaatccaa cgttgcggca
     1141 ggcgtcaacg gggaaggctg gtacccattc gctaagagcg ggcttttccc cgatcctgtg
     1201 cgaagacggt gcagccgctt cgatcaggtg tccgcacgag cgcgctcccg gtacgtccga
     1261 gccttctgct gattgccgca ggtggccatc gagcaccacc ggccggagcg gttccgggag
     1321 tggtcgtaga aggcccagcg gcattcctcg tcggcgcagg ccttcagccg ctgccaggtg
     1381 ccgtcggccg ccgcggtggt gaccgcggcc agcagccggg ccaggccggc gtcgatgccg
     1441 ccgcacacgg gggtcagctc cggccgctcg cccgtgaagc tggcccgcag cgacgcgtgg
     1501 ccggcggccg actccagcgc ggcgaccgcg tcgggggcgg gctcgccccc gttgtggccg
     1561 tccaggaccg cgcgcagccc ctcgcgcacg gtgatggccc gttccagctc ggcctgcgtg
     1621 gcgcgcgcgc ccggctcgca caggccgcgc tcggcgaacc aggcgaccag cgtgtcgggg
     1681 tcggtgagct cgtcgtcctc gggcccctcg tccagctcga ccgtgttcac gaagcgctgg
     1741 accagcgcca gatcgtcggg cgcgcgcttc gcggattctg attcgtggct catcggtcaa
     1801 cagccctgct tatggagcgt atcagcgttc gtaactggct atctagttta gccggtgata
     1861 ctatgtaacc agtgttcagg taaagacggt tacgcaagag gagccatcat gtacagcgag
     1921 atcaccatca ggcacgcaac ggacaacgac gtgaccgccg tgcgccacct ggccgcgctg
     1981 gacgagtccg acgcgccgaa gggcgacgcg ctgctggcct tcgtggacgg cgagctggtc
     2041 gcggcccgct cgaccgaggg ctcggtcgcg gtggccgacc cgttccgtcg cacgcgtcac
     2101 gtgatggagc tgctggacct ccagcgccgg caggagcagg ccgcgtgaac cccatgctca
     2161 catgggagat cgcgtgctgg cacagcaccg aggtgcgccg ggtcagctcg ctgcggcgac
     2221 cgctccaccc ccgccggttc ttcccccgct tctgacgccc cgcggcgctc gccttcctcc
     2281 ctcctaggaa tcaggcgggc gccggggcgc cggaggagtc ctcgacggcc ttgcgggccg
     2341 tctcgaacgc ctccagggcc ttgtccaggt gcgagtgctc gtgggtcgcc atcacgctgg
     2401 tgcgcaggag cgcgccgccg cggggcaccg ccgggtacag cgccacgttg gcgtagacgc
     2461 cggcgtcgta gagggccttc cagaacagca ccgcctccca gtcgtcgccg atggtcaccg
     2521 ggacgatcgg ggtgagcagg tcggagccat cgggcatcgg cgtgggctgc accacgtcga
     2581 agccgcgctc gtccagaccg cgctggaggt accgcgcgtt gtccaggacg cgggccagca
     2641 gctcgcggcc ctcgtcggag cggatcacgc gcagcgccgc cacggtggcg cccaccgcgg
     2701 ccggcacggc cgaggcggtg aacatgaagg cgcgcgactg gacgcgcagg aagtcgatga
     2761 cctcggtggg gccggagatg aagccgccgc aggaggccag cgacttggag aacgtgccca
     2821 tgcgcaggtc cacccggtcg gcgacgccga agacctcgca ggcgcccgtg ccgtcggcgc
     2881 ccagcacgcc gacgccgtgg gcctcgtcca ccatcagccg cgcctcgtag cgctcgcaca
     2941 gctccacgat ctccggcagc ggggccaggt cgccctccat cgagaagacg ccgtcgacca
     3001 ccaccagcac gccgccgccg tccgagcgcg agcggtcgag catcttctcg agcttgtcca
     3061 gcttgttgtg gcggaagggc cggatgcggg cgcgggacat catcaccgcg tccaggatcg
     3121 aggcgtggtc gcccgagtcc acgatcaccg tgtcctcggg gcccagcagc gtggccacgc
     3181 agcccacgtt ggcctggtac ccggtggtga acaccaaggc gtcctcatcg ccggtccagt
     3241 ccgcgatctc gcgctccagc tccaggtgca gcgcggtggt gccgttcagg aaccgtgagc
     3301 ccgtcaggcc ggtgccgtag cgctccaggg cgtccagcgc cccctgcttg accgcggggt
     3361 gggtggtcag gcccaggtag ttgttcgacc cgagcgtgat ccgctcgcgg ccctccatct
     3421 ccacgacggg gccgggctcg ccgtccacct cgcggaagta cgggagcaga tcgtgctcgc
     3481 gggccgcctt cagctgctca aggcggtcgt gggaacgggc tttctcgaac aggtccatta
     3541 gggtcacgcg ccgtgtcgat cgaggtcaca ccggtgcgct cgctacgcga ccggcgggag
     3601 ttcatcgagc tccccttccg cctccatgct agctccgagc agtgggttcc cccgttgcgc
     3661 ctggagcggc acgccttcct cagcccgcgg ctgaacaagt ggttccgcca cgccgacgcc
     3721 cagctgttcc tggcccggcg cggcggccgg gtggtgggcc gggtcagcgc ccaggtcgac
     3781 cacgccttca atgagtacca gggcaacgac tggggcatgt tcggcttctt cgaggccgag
     3841 gacgacgccg aggtggccac ggcgctgctg gacgcggccg ccgcgtggct caccgagcgc
     3901 gggcgcgacc ggatgatcgg gccggtcgac ttcaccatga acgacgaggc gggcctgctg
     3961 atcgagggct acgaccgcga gccgttcatc aagcagccct ggcatccgcc gtactaccgc
     4021 ccgctgctgg agggcgccgg gctggagaag gcgatcgacc tgtggatgtg ggagctgcac
     4081 atctcggacc ggtcgtcgat cgtccagccg atcgtggaca tggccgccca gctcgagccc
     4141 gagcacggga tccgcatccg gcgcatgagc cgccggcggc tgcgcaggga gctggacgtg
     4201 ttcgccgaga cctacaacga ggcgtgggcc gacaactggg ggttcgtgcc ctactcctcc
     4261 gacgacctgg acacctacgc cggcgacctg cagctggtgt tcgaccccaa ctggttcatg
     4321 gtggccgaga agatcgacac gggggagagc gtgggggtgg ccatcacggt gcccgacatc
     4381 aaccaggtgc tcaagcgcat gaacggccgc ctgctgccgc tgggctggtg gcacttcctg
     4441 cgccggcgaa agaccatcga ccgcgtgcgg gtgggcttcc tgggcgtcaa gcgcgactac
     4501 cagcacaccg gcgtcgccgc cgcgttctac atggagcact tcgacaccgc cgcgcgcacc
     4561 ccccagacct ggggcgagat ggggtggatc ctggagacca acgacgccat gaaccggggc
     4621 atggagggca tgggcggccg cgtggtcaag aagtaccgga tgtacgagcg cgaactgccc
     4681 tgaacggatg cgccggagaa gacatcgtgt tcatctccgg cctgagggtt acactcgatc
     4741 ttcttgcccg agccggccga caaccagccc gacgaggagg ccctggacgg tgagctggtg
     4801 gcggaggttc gccacctgcc cgtgcccgcc gcctccggtt ccggcgagga gctggacatc
     4861 cgccggcccg cctcgctg
//
LOCUS       DASUPW010000075         4554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1123772, whole genome shotgun sequence.
ACCESSION   DASUPW010000075 DASUPW010000000
VERSION     DASUPW010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123772"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VFY44_01755"
     CDS             complement(<1..507)
                     /locus_tag="VFY44_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5909185.1"
                     /translation="MSAAATRLTRAEKKAETRTALLRSATTVFAEQGMERASIDDVAR
                     HAGYTKGAFYANFASKEDLFLALLDEHFAELLERLDRAVSGDHDMVDQAREGAASFMS
                     FVAADPEWERLFFDFSFYALRNPGFRAELIRRRGALRDGIATLLQRRVDELGLTPPVP
                     VADIAMMVF"
     gene            complement(504..1808)
                     /locus_tag="VFY44_01760"
     CDS             complement(504..1808)
                     /locus_tag="VFY44_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5909186.1"
                     /translation="MNATATSLPPGPRMPMPLQTLGWTARTMPFLEGAQAKYGDMWTM
                     KLAQEGPWVMVSHPDVVKQVFKGDPEVFRAGEGNAILSPLVGHNSVLTLDGSAHMTQR
                     KLMLPPFHGRRMQAYGELMRDIAQREVDSWPAGGSLTLWPRMQAITLEIILQAVFGLD
                     EGPRLDALRTRLREVLAVSTAPVTMLLIAMLGTRRFERLKVVRRELDPADQLLYEQIR
                     ERREDPDLENRSDILSMLLSARYEDGEPMSDVELRDELVTLLVAGHETTATSLAWTME
                     RLVRHPGALERLRDEVAAGEDEYMDAVVKETLRLRPVLPVVVRRLSRDTEVGGRLLPA
                     GVAVAPCIHLVHRRPDVYPEPSRFRPERFLEQPAGTYTWIPFGGGVRRCLGASFALFE
                     MKQVLTAMMSRIEPSSSATSERVIRRAITMSPSRGGEVTLAA"
     gene            complement(1853..3340)
                     /locus_tag="VFY44_01765"
     CDS             complement(1853..3340)
                     /locus_tag="VFY44_01765"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX5909187.1"
                     /translation="MGSESPLILVSNRGPATFHRDDDGELKPQRGGGGLVTALTGLVH
                     HRDALWIASTMSDEDAEAAREHGNKAFACELDGVSYDIRLVESEPDAYDRFYNTVANP
                     MLWFIQHYLWDLSNAPDIRQWEVESYWEGYRAVNEDLANAVLEEIEGGEDPVVMLHDY
                     HLYAAPRFIREARPDVFLHHFVHIPWTQPDAWRVLPRDIREDIFHSLLSNDVVAFHTR
                     AYRRNFLLCCRHLFDLEVDEEAGVVQFEGREIWVRAYPLPISADTFRRTAQRAKVHEY
                     ERTIMRRKREHLILRVDRADLSKNILRGFAAFDLFLEQHPEFLEKVTFIAHLIPSRQD
                     VAEYAEYLEKIEALVAVVNHRHGTTDWMPIDLRLRENLDEAIAAYKHYDLLMVNAMFD
                     GMNLIAKEGPLVNERHGVSLLSENTGAHEELAEFALSVNPFDVQEQADAIHEALTMSA
                     DERARRAEGLKKVVQARDPGDWVDDQLADIEAKKQGAAPQPTQAA"
     gene            complement(3361..3759)
                     /locus_tag="VFY44_01770"
     CDS             complement(3361..3759)
                     /locus_tag="VFY44_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909188.1"
                     /translation="MSVDSRDVSLAIPAKPGYLVLARLALAAVCRLTPLQATEVADLK
                     LAVTEAAGDFVAEEDDTGPGDAADATPGSDEDRVDFSFRLEEDRLVMELAGTAPASPA
                     VEQELSHAIIEATVDSVDFTPGRTVLVKNL"
     gene            complement(3790..>4554)
                     /locus_tag="VFY44_01775"
     CDS             complement(3790..>4554)
                     /locus_tag="VFY44_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5909189.1"
                     /translation="DLNSDVALLKLDPAGLKLTPLPLGHSSDLTVGTPVAAIGSPFGE
                     RQSLSVGVISALNRSIESLTQFQIGDAIQTDAAINPGNSGGPLLSARGRVLGINSQIK
                     SASGGGEGVGFAVPVDTVRRSLRELRRDGSVSYGYLGVTSQPLYPQLARRLKLPVDTG
                     ALVVDVEDDSPADDAGLGKGGDTIDFQGQTGIPSEGDVVVAVDGRRLTREGDLADRIG
                     RHRAGDKVRLEVLREGKRRTVVVELGKRPEKPPRGG"
BASE COUNT          623 a         1578 c         1663 g          690 t
ORIGIN      
        1 gaagaccatc atcgcgatgt ccgccacggg cacgggcggt gtcaggccca gctcgtcgac
       61 gcggcgctgg agcagggtgg cgatgccgtc gcgcagcgcg ccgcggcggc ggatcagctc
      121 ggcccggaac ccggggttgc gcagggcgta gaaggagaag tcgaagaaca ggcgctccca
      181 ctcggggtcg gcggccacga agctcatgaa gctggccgcg ccctcgcggg cctggtcgac
      241 catgtcgtgg tcgccgctca ccgcgcggtc cagccgctcg agcagctcgg cgaagtgctc
      301 gtccagcagc gccaggaaca ggtcctcctt gctggcgaag ttcgcgtaga acgcgccctt
      361 ggtgtatccc gcgtgccgcg ccacgtcgtc gatcgaggcg cgctccatgc cctgctcggc
      421 gaacacggtg gtggccgacc gcagcagcgc ggtgcgggtc tcggccttct tctcggcccg
      481 cgtgaggcgg gtggcggcgg cgctcacgcg gccagcgtga cctcgccgcc gcgcgacggg
      541 ctcatggtga tcgcgcgccg gatcacgcgc tcggaggtgg cggacgagga gggctcgatg
      601 cgcgacatca tcgcggtcag cacctgcttc atctcgaaca gcgcgaagct ggcgcccagg
      661 cagcgccgca cgccgccgcc gaacgggatc caggtgtagg tcccggccgg ctgctcgagg
      721 aagcgctcgg ggcggaagcg cgagggctcg gggtacacgt cgggccggcg gtgcaccagg
      781 tggatgcagg gcgcgacggc cacgcccgcg ggcagcagcc ggccgccgac ctcggtgtcg
      841 cgcgacaggc ggcgcaccac cacgggcagc acgggccgca gccgcagcgt ctccttgacc
      901 accgcgtcca tgtactcgtc ctcgcccgcg gcgacctcgt cgcggaggcg ctcgagcgcg
      961 ccggggtggc gcaccaggcg ctccatggtc caggccagcg aggtcgcggt ggtctcgtgg
     1021 ccggccacca gcagcgtgac cagctcgtcg cgcagctcga cgtcggacat cggctcgccg
     1081 tcctcgtacc gggcactgag cagcatcgag aggatgtcgc tgcggttctc caggtcgggg
     1141 tcctcgcgcc gttcgcggat ctgctcgtac aggagctggt cggccgggtc gagctcgcgc
     1201 cggaccacct tcaggcgctc gaacctgcgc gtcccgagca tcgcgatcag cagcatcgtg
     1261 accggcgcgg tggagacggc gagcacctcg cgcaggcgcg tgcggagcgc gtccaggcgc
     1321 gggccctcgt ccagcccgaa cacggcctgg aggatgatct ccagcgtgat ggcctgcatc
     1381 cgcggccaca gcgtcaggga gccgccggcg ggccagctgt cgacctcgcg ctgggcgatg
     1441 tcgcgcatga gctcgccgta ggcctgcatc cggcggccgt ggaagggcgg aagcatcagc
     1501 ttgcgctggg tcatgtgcgc ggagccgtcc agcgtcagga ccgagttgtg gcccaccagc
     1561 gggctgagga tggcgttgcc ctcaccggcg cggaacacct cggggtcgcc cttgaagacc
     1621 tgcttgacca cgtccgggtg gctgaccatg acccacggcc cctcctgggc cagcttcatg
     1681 gtccacatgt cgccgtactt ggcctgggcc ccctccagga acggcatcgt ccgggcggtc
     1741 cagcccaggg tctggagcgg catgggcatc cgcgggccgg gcgggaggga ggtggcggtg
     1801 gcgttcacat acccgacggt attcgaatac ccgccggtat gtcaagcgca gatcaggccg
     1861 cttgggtcgg ctgcggcgcc gcgccctgct tcttggcctc gatgtcggcc agctggtcgt
     1921 cgacccagtc gcctgggtcg cgcgcctgga ccaccttctt gagcccctcg gcgcggcggg
     1981 cgcgctcgtc ggccgacatc gtcagcgcct cgtggatggc gtcggcctgc tcctggacgt
     2041 cgaacgggtt cacggacagc gcgaactcgg ccagctcctc gtgggcgccg gtgttctcgg
     2101 acagcagcga cacgccgtgg cgctcgttca cgagcggccc ctccttggcg atcaggttca
     2161 tgccgtcgaa catggcgttg accatcagca ggtcgtagtg cttgtaggcg gcgatcgcct
     2221 cgtccaggtt ctcgcgcagg cgcaggtcga tcggcatcca gtccgtggtc ccgtggcggt
     2281 ggttgaccac tgccaccagc gcctcgatct tctccaggta ctcggcgtac tcggccacgt
     2341 cctggcgcga ggggatcagg tgggcgatga acgtgacctt ctccaggaac tccgggtgct
     2401 gctccaggaa caggtcgaac gccgcgaacc cgcggaggat gttcttggac aggtccgcgc
     2461 ggtccacgcg caggatcagg tgctcgcgct tgcggcgcat gatcgtgcgc tcgtactcgt
     2521 ggaccttggc ccgctgggcg gtgcggcgga aggtgtcggc cgagatcggc agcgggtagg
     2581 cccgcaccca gatctcgcgg ccctcgaact ggacgacgcc cgcctcctcg tccacctcga
     2641 ggtcgaacag gtggcggcag cagagcagga agttgcgccg gtacgcgcgg gtgtggaacg
     2701 ccaccacgtc gttggacagc aggctgtgga agatgtcctc gcggatgtcg cgcggcagca
     2761 cccgccaggc gtcgggctgg gtccacggga tgtggacgaa gtggtgcagg aacacgtcgg
     2821 gccgggcctc gcggatgaac cgcggagcgg cgtacaggtg gtagtcgtgg agcatgacca
     2881 ccgggtcctc gccgccctcg atctcctcga ggaccgcgtt cgccaggtcc tcgttcaccg
     2941 cgcggtagcc ctcccagtag gactcgacct cccactggcg gatgtcgggc gcgttcgaga
     3001 ggtcccacag gtagtgctgg atgaaccaca gcatcgggtt ggcgaccgtg ttgtagaagc
     3061 ggtcgtatgc gtcgggttcg gactcgacca ggcggatgtc gtacgacacg ccgtccagct
     3121 cgcacgcgaa cgccttgttg ccgtgctcgc gggcggcctc ggcgtcctcg tcggacatcg
     3181 tggaggcgat ccagagcgcg tccctgtggt ggaccagccc cgtgagggcg gtgaccagcc
     3241 cgccgccgcc gcgctggggc ttgagctcgc cgtcgtcgtc gcggtggaac gtggccgggc
     3301 cgcggttgga gaccaggatg agaggtgact cggagcccat cgagccttga agttacccgt
     3361 ctacaggttc tttaccagca ccgtgcggcc cggcgtgaag tccacggagt ccacggtggc
     3421 ctcgatgatc gcgtggctga gctcctgctc caccgccgga gaggcgggcg cggtgccggc
     3481 cagctccatg accagccggt cctcttcgag gcggaaggag aagtccaccc ggtcctcgtc
     3541 gctgccgggc gtggcgtcgg cggcgtcgcc cgggccggtg tcgtcctcct cggccacgaa
     3601 gtcccccgcg gcctcggtga cggcgagctt gaggtcggcg acctcggtgg cctggagcgg
     3661 cgtcagccgg cagacggccg cgagcgcgag ccgcgcgagc accaggtacc cgggcttggc
     3721 cggaatggcc agagagacgt cacgactgtc gacactcatc cccgccggga atctattgga
     3781 tgccggggct cagccgcccc ggggcggctt ctcgggccgc ttgcccagct cgacgaccac
     3841 cgtccgccgc ttgccctcgc gcagcacctc gagcctgacc ttgtcgccgg cgcggtggcg
     3901 gccgatgcgg tcggccaggt cgccctcacg ggtcagcctg cgcccgtcca ccgccaccac
     3961 cacgtcgccc tcggacggga tgccggtctg gccctggaag tcgatggtgt cgccgccctt
     4021 gcccagcccg gcgtcgtcgg cggggctgtc gtcctcgacg tcgaccacca gcgcgccggt
     4081 gtccaccggc agcttcaggc ggcgggccag ctgcgggtac agcggctgcg aggtcacgcc
     4141 caggtacccg tagctgacgc tgccgtcgcg gcgcagctcg cgcaacgacc ggcgcacggt
     4201 gtccaccggc acggcgaagc ccacgccctc gccgcccccg ctggccgact tgatctgcga
     4261 gttgatgccc aggacgcgcc cgcgtgcgct cagcagcggc ccgccggagt tgccggggtt
     4321 gatggcggcg tcggtctgga tggcgtcgcc gatctggaac tgggtcagcg actcgatcga
     4381 gcggttcagg gccgagatca cgcccaccga cagcgactgg cgctccccga acgggctgcc
     4441 gatggcggcc accggggtgc ccacggtcag gtcgctcgag tgccccaggg ggagcggcgt
     4501 cagcttgagc cccgcggggt cgagcttgag cagcgcgacg tcggagttca ggtc
//
LOCUS       DASUPW010000076         2650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1678340, whole genome shotgun sequence.
ACCESSION   DASUPW010000076 DASUPW010000000
VERSION     DASUPW010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678340"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1515
                     /locus_tag="VFY44_01780"
     CDS             <1..1515
                     /locus_tag="VFY44_01780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5909190.1"
                     /translation="RLRLPIQVKLALVSAALTFVILLLFAVVVGAFTEQRLKANFDND
                     VRATAADLQERFRVQRQLSGDPVLRVPETTLRAAAAGGATIRVVDRSGKVVAASPGAA
                     NLGPPVEGTSDVGDLRVVARPLFAGSLDRGGNGFGLSPSPIDGAVAFVQYAKPRASLD
                     ATTNRLHLFFALGILGGTGLAFLAGFAVARRAMAPIAGLTRAARLVARTRDPSHRLPR
                     PVANDEVSDLAATLEEMLRELDAARGETEASLQRQREFVADASHELRTPLTSILANLE
                     LLESELDREDGPRDRAAAADIAGSALRSSRRMRRLVGDLLLLARADAGRQAPRTPVDV
                     AQVVRDAALEAAPLAEGHPLSLDLPEDAGVAVVEGVGDDLHRLVLNLVENAFLHTATG
                     TPVVASVRREADQVLLEVADRGPGVPADRRERVFERFARSDEGRTAAGGSGLGLAIVR
                     SVAEAHGGTVVLREAEGGGALFAVTLPARPAHRSDAPGVEDQTAANQAKGAETS"
     gene            1560..2171
                     /locus_tag="VFY44_01785"
     CDS             1560..2171
                     /locus_tag="VFY44_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909191.1"
                     /translation="MALFASLGGVSYGFATGEIDGREIKNGAVTNKDIRNGTVVTKDL
                     RNNEVRGADIRNSTIGGRDVAFNTLGGNDIKESTLTKVPDADNVDGVDSSAFVRKEAP
                     AFTALTLSGGAVTVSGETAPGFDVDTSGYIHLQGTFDPGAAGTQFTLPAGFRPAGTSR
                     FVVPGGAADAAYLTIAPTGEATVASPMDNVSLDGVTFAPSTTP"
     gene            2210..>2650
                     /locus_tag="VFY44_01790"
     CDS             2210..>2650
                     /locus_tag="VFY44_01790"
                     /inference="COORDINATES: protein motif:HMM:NF024734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEX5909192.1"
                     /translation="MASQTHEPRGSARLWALTRRQEGVVSTRQLLDAGFTRAAVKHRV
                     GAGRLHRKARGVYAVGRPDIGRHGEMWVAVLACGPQASISHETGCELYGVRRRNRGPI
                     EVSVPARARRHRAGIRVHNRRVLEGDVTTHRRVPVTSLPLTLIDT"
BASE COUNT          355 a          992 c          960 g          343 t
ORIGIN      
        1 cgcctgcggc tgccgatcca ggtcaagctg gcgctggtct cggcggcgct gacgttcgtg
       61 atcctgctgc tgttcgccgt ggtggtcggc gcgttcaccg agcagcggct gaaggccaac
      121 ttcgacaacg acgtccgcgc gaccgcggcc gacctgcagg agcgcttccg cgtccagcgc
      181 cagctgtcgg gcgacccggt gctgcgcgtt cccgagacca cgctgcgcgc cgcggccgcc
      241 ggcggggcca cgatccgggt ggtcgaccgc agcggcaagg tcgtggccgc ctcgcccggg
      301 gccgccaacc tcggaccgcc ggtcgagggc accagcgacg tcggcgacct gcgcgtggtg
      361 gcccggccgc tgttcgcggg ctcgctggac cggggcggca acggcttcgg cctgtccccc
      421 agcccgatcg acggcgcggt cgcgttcgtc cagtacgcca agccgcgcgc gtcgctggac
      481 gccacgacca accggctgca cctgttcttc gcgctgggca tcctcggcgg caccggcctc
      541 gccttcctgg ccggcttcgc ggtcgcgcgg agggcgatgg cgccgatcgc cgggctgacc
      601 cgcgccgcgc gcctggtggc ccgcacccgc gatcccagcc accgcctccc ccgccccgtg
      661 gccaacgacg aggtcagcga cctggcggcg accctcgagg agatgctgcg cgagctggac
      721 gccgcgcgcg gggagaccga ggcctcgctc cagcgccagc gcgagttcgt agccgacgcg
      781 tcgcacgagc tgcgcacccc gctgaccagc atcctggcca acctggagct gctggagtcc
      841 gagctcgacc gtgaggacgg cccccgcgac cgcgccgccg cggccgacat cgccggctcg
      901 gcgctgcgct cgtcacgacg gatgcgccgg ctggtcggcg acctcctgct gctggcccgg
      961 gccgacgcgg gccgccaggc gccgcgcacg cccgtggacg tcgcccaggt cgtccgcgac
     1021 gcggcgctgg aggccgcccc gctggccgag ggacatccgc tgtcgctgga cctgcccgag
     1081 gacgccggcg tcgccgtggt cgagggcgtc ggcgacgacc tgcaccggct ggtgctcaac
     1141 ctggtcgaga acgccttcct gcacaccgcc accggcaccc cggtggtggc ctccgtgcgg
     1201 cgcgaggccg atcaggtgct gctcgaggtg gccgaccgcg gcccaggggt gccggcggac
     1261 cgccgcgagc gggtgttcga gcgcttcgcc cgctcggacg agggccgcac cgccgccggt
     1321 ggaagcggcc tcggactggc tatcgtgcgt tcggttgctg aggcgcacgg gggcaccgtg
     1381 gtgctgcggg aggccgaagg cgggggcgcg ctgttcgccg tgacccttcc ggcccgccct
     1441 gcgcatcgct cggacgctcc cggtgttgaa gaccagaccg ctgcgaacca agccaaggga
     1501 gctgaaacct cgtgagaatc cgcctgagcc gtccatcgcc cgccctcgtc atcgcctgcg
     1561 tggcgctgtt cgcatcgctg ggcggagtct cctacggctt cgcgacgggt gagatcgacg
     1621 gccgtgagat caagaacggc gcggtcacga acaaggacat ccgcaacggc acggtcgtca
     1681 ccaaggacct gcgcaacaac gaggtccgcg gcgccgacat ccgcaactcc accatcggcg
     1741 gccgcgacgt ggccttcaac accctgggcg gcaacgacat caaggagtcg acgctgacca
     1801 aggtgcccga cgccgacaac gtcgacggcg tggacagcag cgcgttcgtg cgcaaggagg
     1861 cgccggcgtt cacggcgctg acgctcagcg gcggcgcggt cacggtcagc ggcgagacgg
     1921 cgccgggctt cgacgtggac accagcggct acatccacct gcagggcacg ttcgatccgg
     1981 gcgcggcggg cacccagttc acgctgccgg cgggcttccg tcccgcgggg acgagccgct
     2041 tcgtggtccc cggcggcgcg gcggacgcgg cttacctgac gatcgcgcca acgggcgagg
     2101 ctaccgtggc ttcgccgatg gacaacgtct cgctcgacgg cgtgacgttc gcgccatcca
     2161 ccaccccctg agggggcgaa cataacgccg ttcatgcctt tgggaggtga tggcctccca
     2221 aacgcatgaa ccgcgcggct cggcgcggct ctgggcgctg acccgcaggc aggagggggt
     2281 ggtcagcacc cggcaactgc tcgacgccgg cttcacacgc gccgccgtga agcaccgggt
     2341 cggcgccggc cggctacacc gaaaggcgcg cggcgtctac gccgtcggcc ggccggacat
     2401 cggccgccac ggggagatgt gggtcgccgt cctggcgtgc gggccacaag cgagcatcag
     2461 ccacgagacg ggctgcgagc tgtacggggt acggcggcgc aatcggggcc cgatcgaggt
     2521 gtcggtgccc gcccgcgccc gccggcaccg tgccggcatc cgggtgcaca accggcgcgt
     2581 tctcgaggga gacgtgacga cccaccgtcg cgtgcccgtc acctccctcc cgttgacgct
     2641 catcgacacc
//
LOCUS       DASUPW010000077         2677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_432510, whole genome shotgun sequence.
ACCESSION   DASUPW010000077 DASUPW010000000
VERSION     DASUPW010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432510"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2071)
                     /locus_tag="VFY44_01795"
     CDS             complement(<1..2071)
                     /locus_tag="VFY44_01795"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5909193.1"
                     /translation="MSDEPADTDATPERGRVWMLEERGRERELVFAFAFDEDLNEAVK
                     ELPRRWFDWRRKHWRVPADPRAAKEVEQLLARFPDLQPEAEVLSWLSNSDKWRGLVTV
                     LAHEGAGAFVVRTLSGDVPEELTDDAIEVTEDRHVLPFSRASARLVTQLEGTDLDSLA
                     IGCVREIREGIEPAGAEMSLEVGEDGEPTLTLATIWDHAPARDFKALPEAELVALPGR
                     FFKREAAWGVAVPADPALAPPIGEFVWAHETVRLDDRARDTLDELSEEHDRASATVAL
                     SYAEDADLDGLELGGTLRPFQRAGVRYALERRRTFIADEQGLGKTVQALGALKADDAF
                     PAIVVCPASMKLMWEREANKWLPERKVAILSGRTEAGWPEEADGADILVVNYDILDAH
                     FDRLAARHAEALVLDESHYVKNPQAQRTKAALELARRLPEDALRLALTGTPILNRPEE
                     LVAQLRVLDRLKDFGSGARLARRFRSAGSDDRLHWNLRAHCYVRRTKAQVLPQLPAKT
                     QDTVPIRLSNENDYRLAESDVIAWLQSLPMDLKTMDAKVAAAARAEQLVRLNSLRQLA
                     AAGKLPSALAWIEDFLQSGEPLVVFAEHVATQKAVMEHFPKAAHILGADSAVRRQEAV
                     DAFQDPDGPQLIVCSMKAASQGITLTRASNVAFLELDWTPARHDQAEDRLHRIGQDSA
                     VTAWYLLA"
     gene            2216..>2677
                     /locus_tag="VFY44_01800"
     CDS             2216..>2677
                     /locus_tag="VFY44_01800"
                     /inference="COORDINATES: protein motif:HMM:NF024734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEX5909194.1"
                     /translation="MRIAATQFGVVTRAQLLAAGLSSSAIGRRVAGGLLVPLYRGVYA
                     VGHTAITRQGRALAAQLACGDQAVLGFEDAAAHMGLMAPTSPVWHIVVPRPSPRGHSG
                     IRVHRMRLAERDIVTDGPLRHTSLARTALDLAAAGPERRVRRLLREAEIRRV"
BASE COUNT          332 a          980 c          970 g          395 t
ORIGIN      
        1 gggccagcag gtaccaggcg gtcacggcgc tgtcctggcc gatccggtgc agccggtctt
       61 cggcctggtc gtgacgggcg ggggtccagt ccagctccag gaacgccacg ttggacgcgc
      121 gcgtcagcgt gatgccctgt gacgccgcct tcatcgagca cacgatcagc tgcgggccgt
      181 cggggtcctg gaaggcgtcc accgcctcct ggcgcctgac cgcggagtcg gcgccgagga
      241 tgtgcgccgc cttggggaag tgctccatca cggccttctg ggtggccacg tgctcggcga
      301 acaccaccag cggctcgccc gactggagga agtcctcgat ccaggccagc gcgctgggca
      361 gcttgccggc ggcggccagc tggcgcaggc tgttcaggcg caccagctgc tcggcgcggg
      421 ccgcggcggc caccttcgcg tccatggtct tcaggtccat cggcagcgac tgcagccagg
      481 cgatcacgtc ggactcggcc agccggtagt cgttctcgtt cgacagccgg atgggcacgg
      541 tgtcctgggt cttggcgggg agctggggca gcacctgcgc cttggtgcgg cgcacgtagc
      601 agtgcgcgcg caggttccag tgcaggcggt cgtcgctgcc ggcgctgcgg aaccggcgcg
      661 ccagccgggc gccgctgccg aagtccttga gccggtccag cacgcgcagc tgggccacca
      721 gctcctcggg ccggttcagg atcggcgtgc cggtcagcgc caggcgcagc gcgtcctcgg
      781 gcaggcgccg cgccagctcc agcgccgcct tggtgcgctg ggcctgcggg ttcttcacgt
      841 agtgcgactc gtccagcacc agcgcctccg cgtggcgggc ggccaggcgg tcgaagtgcg
      901 cgtccaggat gtcgtagttc acgaccagga tgtcggcgcc gtcggcctcc tcgggccagc
      961 cggcctcggt gcggccgctc aggatcgcca ccttgcgctc ggggagccac ttgttggcct
     1021 cgcgctccca catcagcttc atcgaggcgg ggcagaccac gatcgccggg aacgcgtcgt
     1081 cggccttcag cgcgcccagg gcctggacgg tcttgcccag cccctgctcg tcggcgatga
     1141 aggtgcgccg gcgctccagc gcgtagcgca cgccggcgcg ctggaacggg cgcagcgtgc
     1201 cgcccagctc caggccgtcc aggtcggcgt cctcggcgta ggacagcgcc accgtggccg
     1261 acgcgcgatc gtgctcctcg ctcagctcgt ccagcgtgtc gcgggcgcgg tcgtccagcc
     1321 ggaccgtctc gtgggcccag acgaactcgc cgatcggagg cgccagcgcg gggtcggccg
     1381 ggaccgcgac gccccaggcg gcctcgcgct tgaagaagcg gccgggcagg gccaccagct
     1441 cggcctcggg cagggccttg aagtcacgcg cgggcgcgtg gtcccagatc gtggccaggg
     1501 tcagggtggg ctcgccgtcc tcgccgacct ccaggctcat ctccgcgccg gcgggctcga
     1561 tgccctcgcg gatctcgcgc acgcagccga tggccagcga gtccaggtcg gtgccctcca
     1621 gctgggtcac cagccgcgcg ctcgcgcggc tgaagggcag cacgtgacgg tcctcggtga
     1681 cctcgatcgc gtcgtcggtc agctcctcgg gcacgtcgcc gctgagcgtc cgcaccacga
     1741 acgccccggc gccctcgtgc gccagcaccg tgaccagccc gcgccacttg tccgagttcg
     1801 acagccacga caagacctcg gcctcgggct ggaggtcggg gaagcgcgcc agcagctgct
     1861 cgacctcctt cgccgcccgc gggtcggcgg gcacgcgcca gtgcttgcgg cgccagtcga
     1921 accaccgccg cggcagctcc ttgacggcct cgttcaggtc ctcgtcgaac gcgaacgcga
     1981 acaccagctc ccgctcgcgc ccccgctcct cgagcatcca cacgcgcccc cgctcgggcg
     2041 tggcatcggt gtcggccggc tcgtcagaca tcaatcgatc cagattagtg gggtccgggg
     2101 tcgattgggt cacgttgttc ccgctgggcg ggaacaatcc gtcactcggg gcggccaagg
     2161 ggggcgaagc cgggccactc gatggcttgt tcccggtgtg cgggatcaat gcatcgtgcg
     2221 gatagcggcc acccagttcg gcgtcgtgac gagggcgcag ctcctggcgg ccggcctcag
     2281 cagctcggcc atcggccggc gggtggcggg tggcctgctg gtccccctgt atcggggcgt
     2341 gtacgccgtc ggccacactg cgatcacgcg acagggacgg gcgctggcgg cgcagctggc
     2401 gtgtggcgac caggccgtcc tgggattcga ggacgccgcg gctcacatgg gcctgatggc
     2461 cccaacctcc ccggtgtggc acatcgtcgt gccccgaccg agccctcgcg gtcactccgg
     2521 catccgggtc caccgcatgc gcctggccga acgggacatc gtcaccgacg ggccgctccg
     2581 tcacacctcg ctggcgcgta ctgcgctcga cctggcggcc gccgggccgg agcgacgagt
     2641 tcgccggctg cttcgcgagg ccgagatccg gcgcgtg
//
LOCUS       DASUPW010000078         2584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1678469, whole genome shotgun sequence.
ACCESSION   DASUPW010000078 DASUPW010000000
VERSION     DASUPW010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678469"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1317)
                     /locus_tag="VFY44_01805"
     CDS             complement(139..1317)
                     /locus_tag="VFY44_01805"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEX5909195.1"
                     /translation="MSTEEQAVPFGNYQYEIYLKGLGGETPALPIDYDDLEALAAERL
                     SPEAFHYAAGGAGEGRSMTANRRAFDAWQILPRMLGDVSVRDLSTDILGTRFPAPVML
                     GPVGVLSILHEDAELAVARAAARTGVPMCLSTAASSTMEDVAGELGDTPGWYQLYWPS
                     DPELAESLVRRAGAAGYRAIVVTLDTRMMPWRPRDLNGAYLPFLRGEGIANYLSDPVF
                     RARCDEPPEENMQAAIGQWAQVFPNPTLSWEDLARLREWTDLPVLVKGVLHPDDARRA
                     VEQGAQGVVVSNHGGRQIDGSATPLGQLPAVVDAVGDGTAVLLDGGVRTGSDVLKAVA
                     LGARAVLVARSYVWGLALGGDAGVEATLRNLLADTDLTLAMSGLSSIGQVDRDLLVAA
                     "
     gene            complement(1328..2206)
                     /gene="ligD"
                     /locus_tag="VFY44_01810"
     CDS             complement(1328..2206)
                     /gene="ligD"
                     /locus_tag="VFY44_01810"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX5909196.1"
                     /translation="MTDVLRIGRRDVRFTHPDKVLFPGAKLTKLDLARHYERVAPVML
                     PYVKDHPLALQSYPGGIGGKGFFVKAVPDYFPEWVERVTVPKRGGTITHALAADAATL
                     VYLAGQNVITPHQWLSRADEPKQPDRLIIDFDPSKEGGFADVRAAARDAGERLRDAGL
                     ATYAMVTGSRGIHVVCPLRRGPGFGDTHTFARAVAEQMVEADPRHLTLEYKKADREQK
                     IFVDVNRNAYAQHAVSPYGVRPRDRAPVATPIHWEELSDPKLKPDRWTVKTIAARIES
                     EGDPWKGMSRRARNLP"
     gene            2256..>2584
                     /locus_tag="VFY44_01815"
     CDS             2256..>2584
                     /locus_tag="VFY44_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909197.1"
                     /translation="MALAAGCGGVGRTTKDGRVGDTLTSGGLRVTVLKTDATVPRPES
                     DVTGLSTPGPGKTFFGAKVKACTDRDQAIKAYDFQLDPAGGDAVRARLPQRSYDDSFD
                     VTRSGCES"
BASE COUNT          345 a          953 c          934 g          352 t
ORIGIN      
        1 gctggacgcc gcgaccgaac aggtccaggc ctcctacagg atgcccgccg accgtggcac
       61 gggcgccgcg ctggccgccg gccggctctg ggtggtcaac caccgccgcc cgcgtcaggg
      121 cgagggcgag tacctggact aggcggccac cagcaggtcc cggtccacct ggccgatcga
      181 gctcaacccg gacatggcca gcgtcaggtc cgtgtcggcc agcaggttgc gcagcgtggc
      241 ctccacgccg gcgtcgccgc ccagcgccag gccccacacg taggagcgcg ccaccagcac
      301 ggcgcgcgcg cccagcgcca ccgccttgag cacgtcggat cccgtgcgca cgccgccgtc
      361 cagcagcacg gccgtgccgt cgcccaccgc gtcgaccacc gcgggcagtt ggccgagggg
      421 cgtcgccgag ccgtcgatct gacgtccgcc gtggttggac accacgacgc cctgggcgcc
      481 ctgctcgacc gcccggcggg cgtcgtcggg gtgcagcacg cccttgacca gcaccggcag
      541 gtcggtccac tcacgcagtc gagccaggtc ctcccagctc agcgtcgggt tggggaacac
      601 ctgggcccac tggccgatgg cggcctgcat gttctcctcg ggcggctcgt cgcaacgggc
      661 gcggaacacc gggtcgctca ggtagttcgc gatgccctcg ccgcgcagga acggcaggta
      721 cgcgccgttc aggtcgcgcg ggcgccaggg catcatgcgg gtgtccagcg tgaccacgat
      781 ggcccggtat cccgcggccc cggcgcggcg caccagcgac tcggccagct ccgggtcgct
      841 cggccagtag agctggtacc agccgggcgt gtcgcccagc tctcccgcga cgtcctccat
      901 ggtcgaggag gccgcggtgc tcaggcacat gggcacgccg gtgcgggcgg cggcacgggc
      961 cacggccagc tcggcatcct cgtgcaggat cgacaggacc ccgaccgggc ccagcatcac
     1021 cggcgcgggg aagcgcgtgc ccaggatgtc ggtgctcaga tcgcgcacgc tcacgtcgcc
     1081 cagcatccgg ggcaggatct gccacgcgtc gaacgcgcgc cggttcgcgg tcatgctccg
     1141 gccctcgccg gcgccaccgg cggcgtagtg gaacgcctcg ggagacagcc gctcggcggc
     1201 cagcgcctcc aggtcgtcgt agtcgatcgg cagcgcgggg gtctcgcccc caagcccctt
     1261 gaggtagatc tcgtactggt agttgccgaa cgggacggcc tgctcctcgg tgctcatcgg
     1321 cgccagccta cggcaggttc cgcgcccgcc ggctcatgcc cttccacggg tcgccctccg
     1381 actcgatccg cgccgcgatg gtcttcacgg tccagcggtc gggcttgagc ttggggtccg
     1441 agagctcctc ccagtggatc ggcgtggcca cgggcgcccg gtcgcgcggg cgcacgccgt
     1501 agggcgacac cgcgtgctgg gcgtaggcgt tgcggttcac gtccacgaag atcttctgct
     1561 cgcggtcggc cttcttgtac tccagcgtca ggtgcctggg atcggcctcg accatctgct
     1621 cggccaccgc gcgggcgaac gtgtgggtgt cgccgaatcc cggcccgcgg cgcagcgggc
     1681 acaccacgtg gatgccgcgc gagccggtga ccatggcgta ggtggccagc ccggcgtcgc
     1741 gcaggcgctc gccggcgtcg cgggcggcgg cgcgcacgtc ggcgaacccg ccctccttgg
     1801 aggggtcgaa gtcgatgatc agccggtccg gctgcttggg ctcgtccgcg cgcgagagcc
     1861 actggtgcgg cgtgatcacg ttctggcccg ccaggtacac cagcgtggcc gcgtcggcgg
     1921 ccagcgcgtg cgtgatcgtg ccgccgcgct tgggcacggt cacgcgctcg acccactccg
     1981 ggaagtagtc cggcaccgcc ttgacgaaga agcccttgcc cccgatcccg ccggggtagg
     2041 actgcagcgc cagcgggtgg tccttgacgt agggcagcat caccggcgcc acgcgctcgt
     2101 agtggcgtgc caggtccagc ttggtcagct tcgcgcccgg gaacaggacc ttgtccgggt
     2161 gggtgaacct gacgtcgcgg cggccgatcc tcagaacgtc ggtcatgctg ccctgcagca
     2221 tggcgcaacc gcgggcactg cctctcctcg cagccgtggc gctggccgcc gggtgcggcg
     2281 gcgtgggcag gaccaccaag gacgggcggg tgggggacac gctgaccagc ggaggcctgc
     2341 gcgtcacggt gctgaagacc gacgccacgg tgccacggcc cgagagcgac gtcaccgggc
     2401 tgagcacgcc cgggcccggg aagacgttct tcggcgcgaa ggtcaaggcg tgcaccgacc
     2461 gcgaccaggc gatcaaggcg tacgacttcc agctcgatcc ggccggcggg gacgcggtcc
     2521 gggcgcgcct gccccagcgc tcctacgacg acagcttcga cgtgacccgt agcggctgcg
     2581 aatc
//
LOCUS       DASUPW010000079         8882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1817360, whole genome shotgun sequence.
ACCESSION   DASUPW010000079 DASUPW010000000
VERSION     DASUPW010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8882
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1817360"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="VFY44_01820"
     CDS             complement(<1..829)
                     /locus_tag="VFY44_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909198.1"
                     /translation="MAAAKRNFPRSPSARRTAPAVGLALAAAVLAAGPVAPAFAQGAT
                     GTAAATTQAGTGSSRLALEGRGLRGLRGLGVRMSATRPVRVSATALTLPVSTGALAST
                     VRLDSKGALVLRKGRRKLRLSSLQVRLAGKGSITARTGKRRITLFRVTPARGKAMRIN
                     AATGRATLQSARVTMSKGTGRRIRKALKLRRTPSGSFGTVTVIATFRPAAPLGKPLPG
                     PEPPVLARPATAVDVRVDGIVWEPKESFVNYMNTQAAPGGGTYTSGGAAPAAGQPENR
                     "
     gene            complement(881..1681)
                     /locus_tag="VFY44_01825"
     CDS             complement(881..1681)
                     /locus_tag="VFY44_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5909199.1"
                     /translation="MERGDTALEVTDLRVDLGGKTVLDGAGLTLRAGELVTVVGPNGA
                     GKSTMVRAAAGLQRFERGSVEWMGRSLDSLRGRRLALIRAFVPQRGRVPEGVTVLDAV
                     TIGRSAHLRVLQRATRADREAVDRAMARSGVEGFRDRQLSTLSGGELQRVQIAVALAQ
                     EAPVLMADEPTSQLDLGATVDVAKLLRGLADDGLAVLLVAHDLALAAAVADSVVVIHR
                     GRSVATGAPAEALSADRLREVWAVDASLEARDRHTALHVAWLGGARNR"
     gene            complement(1681..2667)
                     /locus_tag="VFY44_01830"
     CDS             complement(1681..2667)
                     /locus_tag="VFY44_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX5909200.1"
                     /translation="MVLRRTAVLAVGLVALVCGFAASLALGAVHVPLSDVIDALLGKA
                     DPGPFKQIVLTLRLPRTVEAVVVGAGLGVAGALLQGALANPLASPDVIGVTAGAGFGA
                     VLILLVFPGSIALLPVGALVFGLLAAALVFAIAWTGQNAGGVGRLILAGIAMAALFTA
                     GTTSLMTAYPDRVQSVIFFIAGGLTSEGWRELEVVWPYLAVGFVMALALIRPLDRLAL
                     GDDVAASLGARPRLIRLAAGIAAALLAAAAAALAGLLGFLGLIVPHVVRLAGGTAGHA
                     FVVPASAVGGAALLVAGDTLARTVRAPIELPVGPLMVVIGVPLFLWLLRKAV"
     gene            complement(2667..3515)
                     /locus_tag="VFY44_01835"
     CDS             complement(2667..3515)
                     /locus_tag="VFY44_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5909201.1"
                     /translation="MAAIALAALVASALPAAPAGAAAPKRIIALTPFTANTLAALGVK
                     PVAIGQTLGGRERFNKRLRGVRSLPMGHPIGPNLEQVASLNPQLVFNSPTFRKGSAGM
                     RELKIKVVESDPTNIAESVAQTRRIGRIVGKRKQADKLALKLLKGVARARQRIRDRPT
                     VLVILGVGRTPFAFLRNSWGGDVVRNAGGELLTGGRNDRGGFARISDEVVVAEDPDVI
                     IAVPHAAAKDIPSIARYLRSNPAWSTTRAVREKRVYVSTDNSLLQAGTDAGTVIREVR
                     KKYLKN"
     gene            complement(3792..7373)
                     /gene="metH"
                     /locus_tag="VFY44_01840"
     CDS             complement(3792..7373)
                     /gene="metH"
                     /locus_tag="VFY44_01840"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX5909202.1"
                     /translation="MARDYLKTVNERVVVFDGGMGATLEQFDLTQEDYMGLQGKCHEA
                     LVLQRPDVIEGVHTSMIEAGADVLETDTFQASRIKLEEWGLEDHTVEINQKAAEIARK
                     AAGEDNFVAGSIGPTGHLPASDDPTLGKILFSELVEVFTEQAEGLINGGVDLIIIETA
                     QDILEVKAAVFGARAAMESTGREVPIQCSVSLLPNGGKMLLGTDIEAVLTTLDALDVD
                     VIGLNCSTGPDDMRDAIRYLGENCPVPVHCIPNAGIPYQGPDGETIFPAEPEPLADVL
                     AEFVEKNGVSIVGGCCGTTPDHIKAIADRVKDKQVKPRPERGELKVSSMMSATPLAQE
                     PAPTIVGERVNSQGSRKAKEMLLADDYDSLLNLATSQVEGGAHVLDLCVALTEREDED
                     EQMRQVAKIVSLSLPAPLQIDSTEPEVMKLALEQTPGRAIVNSVNLEAGRDKLDKVVP
                     LVKAHGAALIALTIDEEGMAKTRDRKVEIAQRIRDMVVDEHGLDEESLIFDVLTFTLT
                     TGDEEWRPSAVETIEGIRELKKALPKVKTSLGVSNVSFGVSPKARKILNSVFLHHCVE
                     AGLDLAMVHPADVTPYGEISEEERKLTDALVFNESEDALEKFIGHFESQGDEAEAEAE
                     NPTEGMEPEEALHWHILRRKKEGVEEWIDKSNEKIGAVPTLNTVLLPAMKEVGDKFGS
                     GELILPFVLQSAEVMKRAVAQLENYLEKMEGHTKGTVVLATVFGDVHDIGKSLVNTIM
                     TNNGYTVVDLGKQVPVDTILSAAEEHKADAIGLSALLVSTSKQMPIAVGELHDRELDY
                     PVLVGGAAINRDFGRRMLYPKGKESDEIYEPGVFYCKDAFQGLDTMDALMEPDQREEL
                     MEKTRAAAKELREKPVVEDDSPPVTDDSVRSPVDNSNEVPEPPYWGVQEIDADLGDVY
                     PYLDRHVLFKLHWGGRGKKGEEWRKIIEGDGDDEGFKPKLERMWAEQDYLEPKVKVGY
                     FPCNSEGNDLVIFDPEDQDKEIERLTFPRQPKHDRICLADFYKPIDSGERDVVALQGV
                     TVGPKSTDVMAQLEKDGEFSEQLFTHGLAVQAAEGLAEWSHHNSREAFGIDPDQGRRY
                     SWGYPACPDQSEHEKVWRLLDLDSIGMYLSDGFAVMPEQSTVAIVSHHPQSVYFGMKS
                     GKIPAEKKPDELIAGTERGGELPPDEDPTEGAREAEVGDETAEPTPA"
     gene            complement(7429..>8882)
                     /locus_tag="VFY44_01845"
     CDS             complement(7429..>8882)
                     /locus_tag="VFY44_01845"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5909203.1"
                     /translation="QGKRDLALAARRVAADAFAANDLPGEAAVERLVAGSYLQAAGRH
                     TEAAATARTAAEEAARCDRADLRARAMGLEGVARVKQGSFAEGIEILRGGLSLALDRG
                     LTLEAAEVYQRLGTAHEVAGDYAKARESLGTAITLCERAPGGGLEHTCLSCMAYVLRE
                     LGDWDQVEELCDGLLTPDSGPDDTLVADGVLGAVHLWRGRPEQARPLLTRCLETATRL
                     DVVSMQCDSAAALAWLAAEEGDDDRVRELCRLVLERWSRSEDHHYAVWGLRWSAAWFA
                     RHDAAADARACAEALSQIAGSAGYPDALAALAHALGEAALAEGDPVAAAEQLTRAIEL
                     HAGLDIPFERAQVLLRAGVVLAAAGEPEAALERLAEAHRVATMLGAAVLAGDVAAEVA
                     AQGASVEDLLGRRAAAAHEHGGLSRREVEVVRLVAGGLTNREIAERLVLSVRTVDMHV
                     RSILTKLRCRTRTEAAGRAGELGLLEQGVESGG"
BASE COUNT         1164 a         3276 c         3146 g         1296 t
ORIGIN      
        1 cccggttctc gggctggccc gccgccggcg ccgcaccgcc cgaggtgtag gtgccgccgc
       61 cgggggcggc ctgggtgttc atgtagttca cgaacgactc cttgggctcc cagacgatgc
      121 cgtccacgcg cacgtcgacc gcggtcgcgg gccgggccag gaccggcggc tcggggcccg
      181 gcagcggctt gccgagcggg gcggccgggc ggaaggtggc gatcacggtc acggtcccga
      241 agctgcccga cggcgtccgc cgcagcttca gcgccttcct gatccggcgg ccggtgccct
      301 tggacatggt cacccgggcc gactggagcg tggcccggcc ggtggccgcg ttgatgcgca
      361 tcgccttgcc cctggcgggg gtgacgcgga agagggtgat ccggcgcttg cccgtcctgg
      421 ccgtgatcga tcccttcccg gccagcctga cctgcagcga cgacagcctg agcttgcgcc
      481 ggcccttgcg cagcaccagc gcgcccttgc tgtccaggcg caccgtggag gccagggcgc
      541 cggtggacac cggcagggtc agcgccgtgg ccgagacccg gaccggcctg gtggcggaca
      601 tcctcacgcc caggccgcgc aggcccctca gccccctgcc ctccagcgcg agacggctcg
      661 agccggtgcc ggcctgggtg gtggcggcag cggtgccggt cgccccctgc gcgaaggcgg
      721 gggcgaccgg accggccgcg agcacggccg ccgcgagcgc caagccgacg gcgggcgctg
      781 tcctgcgcgc cgacgggctc ctggggaagt tgcgcttcgc tgcggccatg acgtcctatc
      841 aactgttagg cgaccctaat caggccaccc taacaaggag tcagcggttg cgcgcgcctc
      901 ccagccatgc cacgtgcagg gcggtgtggc ggtcgcgcgc ctccagcgac gcgtcgacgg
      961 cccagacctc gcgcaggcgg tcggcgctca gcgcctcggc gggtgcgccg gtggccaccg
     1021 accggccccg gtggatgacc acgaccgagt cggccacggc cgccgcgagc gccaggtcgt
     1081 gcgccaccag cagcaccgcc aggccgtcgt cggccagccc gcggagcagc ttggccacgt
     1141 ccaccgtggc gcccagatcc agctgcgagg tgggctcgtc ggccatcagg accggggcct
     1201 cctgggccag cgccacggcg atctggacgc gctggagctc gccgcccgag agcgtggaca
     1261 gctggcggtc gcggaagccc tcgaccccgg accgggccat ggcgcggtcg accgcctcgc
     1321 ggtccgcgcg cgttgcgcgc tggagcacgc gcaggtgtgc cgagcggccg atggtcacgg
     1381 cgtcgagcac ggtgaccccc tcgggcacgc ggccgcgctg gggcacgaac gcccggatca
     1441 gggccaggcg ccggccgcgc agggagtcga gcgaccgccc catccactcc accgacccgc
     1501 gctcgaagcg ctggagcccc gcggcggcgc gcaccatggt cgacttgccg gccccgttgg
     1561 ggcccacgac cgtgaccagc tcgccggcgc gcagcgtcag cccggcgccg tccaggacgg
     1621 tctttccgcc caggtccacc cgcaggtcgg tgacctcgag cgcggtgtcg ccgcgctcca
     1681 tcagacggcc ttccgcagca gccacaggaa cagcggcacg ccgatgacca ccatcagcgg
     1741 gcccacgggc agctcgatgg gcgcccgcac cgtgcgcgcc agcgtgtcgc ccgccaccag
     1801 cagggcggcc ccgcccacgg ccgaggccgg gaccacgaac gcgtgcccgg ccgtgccgcc
     1861 ggccaggcgc accacgtgcg ggacgatcag ccccaggaag cccagcagcc cggccagcgc
     1921 ggcggccgcg gcggccagga gcgcggccgc gatccccgcc gccaggcgga tcagccgcgg
     1981 gcgggcgccc agcgaggcgg ccacgtcgtc gcccagggcc agccggtcca gcggccggat
     2041 cagggccagg gccatcacga accccaccgc caggtagggc cagaccacct cgagctcgcg
     2101 ccagccctcc gaggtcaggc cgccggcgat gaagaagatc accgactgca cgcggtcggg
     2161 gtaggcggtc atcagcgatg tcgtgccggc ggtgaacagc gcggccatgg cgatgccggc
     2221 caggatcagc cgcccgacgc cgcccgcgtt ctgccccgtc caggcgatgg cgaacaccag
     2281 cgccgcggcc agcaggccga acaccagcgc gccgaccggc agcagggcga tcgagcccgg
     2341 gaagaccagc aggatcagca ccgcgccgaa gcccgccccg gcggtcacgc cgatcacgtc
     2401 gggcgaggcc agcgggttgg ccagcgcccc ctgcagcagc gctccggcca ccccgagccc
     2461 ggcccccacc accacggcct cgaccgtgcg cggcaggcgc agggtcagca cgatctgctt
     2521 gaacgggccc ggatccgcct tgcccagcag cgcgtcgatc acatccgaca gcggcacgtg
     2581 caccgctccc agcgccagcg aggcggcgaa gccgcagacc agggccacca gccccaccgc
     2641 caggacggcg gtcctgcgca agaccatcag ttcttcagat acttcttgcg gacctcgcgg
     2701 atcaccgtgc cggcgtcggt gccggcctgc agcagcgagt tgtcggtgga cacgtagacg
     2761 cgcttctcgc gtaccgcccg cgtcgtggac cacgccgggt tcgagcgcag gtagcgcgcg
     2821 atcgagggga tgtccttggc cgcggcgtgg ggcacggcga tgatcacgtc cgggtcctcg
     2881 gccacgacga cctcgtccga gatgcgcgcg aagcccccgc ggtcgttgcg cccgccggtg
     2941 agcagctcgc cccccgcgtt gcgcaccacg tccccacccc agctgttgcg caggaacgcg
     3001 aacggcgtcc gcccgacgcc caggatcacg agcacggtgg gacggtcgcg gatcctctgc
     3061 cgcgcccggg ccacgccctt gagcagcttc agggccagct tgtcggcctg cttgcgcttg
     3121 ccgacgatcc ggccgatgcg gcgcgtctgg gccaccgact cggcgatgtt ggtcgggtcg
     3181 ctctcgacca ccttgatctt gagctcgcgc atgcccgcgg agcccttcct gaaggtcggc
     3241 gagttgaaca ccagctgcgg gttcaggctg gccacctgct ccaggttcgg cccgatcggg
     3301 tgccccatgg gcagcgagcg cacgccccgg agccgcttgt tgaaccgctc gcggccgccc
     3361 agcgtctggc cgatggccac cggcttcacg cccagcgccg ccagcgtgtt tgcggtgaac
     3421 ggcgtcagcg cgatgatccg cttcggcgcc gcggcgcccg cgggcgcggc aggcagcgcg
     3481 gatgcgacca gtgcggccag ggcgatagcg gccactcgcc gcagggtgct catcatgatt
     3541 agggcagcct aacaagggat gcttaggcgc gcctaacaac gtctgaggcc ccgtgaccgg
     3601 ggaggtggtc acggggcctc agtgtgaagg cggggccggg aactcaggcc tgtcgtgggg
     3661 tcgcgatggg cgtttccgct gtgcgagtcg cgatggcgtt ctgtatgagc acatactgcg
     3721 cccgccacga cgcccgcgca tcgcccgcga tgacgatctt cgggtcctac ctacggatga
     3781 tcccgcgacc cctacgccgg cgtgggctcg gccgtctcgt cgccgacctc ggcctcgcgc
     3841 gcaccctcgg tggggtcctc gtcgggcggc agctcgccgc cgcgctcggt gccggcgatg
     3901 agctcgtcgg gcttcttctc ggccgggatc ttgcccgact tcatgccgaa gtagaccgac
     3961 tgcgggtggt ggctgacgat ggcgaccgtg gactgctcgg gcatcacggc gaagccgtcg
     4021 gacaggtaca tcccgatcga gtccaggtcc agcaggcgcc agaccttctc gtgctcggac
     4081 tggtccgggc acgcggggta gccccaggag tagcggcggc cctggtcggg gtcgatgccg
     4141 aacgcctcgc gcgagttgtg gtgcgaccac tcggccagcc cctcggccgc ctgcacggcc
     4201 aggccgtgcg tgaacagctg ctcggagaac tcgccgtcct tctccagctg ggccatcacg
     4261 tcggtggact tcgggccgac ggtcacgccc tggagcgcga ccacgtcacg ctcgccggag
     4321 tcgatcggct tgtagaagtc ggccaggcag atccggtcgt gcttgggctg gcgcgggaac
     4381 gtcagccgct cgatctcctt gtcctggtcc tccgggtcga agatgaccag gtcgttgccc
     4441 tcgctgttgc aggggaagta gccgaccttg accttgggct cgaggtagtc ctgctcggcc
     4501 cacatgcgct cgagcttggg cttgaagccc tcgtcgtcgc cgtcgccctc gatgatcttg
     4561 cgccactcct cgcccttctt gccgcggcca ccccagtgca gcttgaacag cacgtggcgg
     4621 tcgaggtagg ggtagacgtc gccgaggtcg gcgtcgatct cctgcacgcc ccagtagggc
     4681 ggctcgggga cctcgttcga gttgtccacg ggggagcgta ccgagtcgtc ggtcacgggc
     4741 ggcgagtcgt cctcgaccac gggcttctcg cgcagctcct tggccgcggc acgggtcttc
     4801 tccatcagct cttcgcgctg gtcgggctcc atcagggcgt ccatcgtgtc caggccctgg
     4861 aaggcgtcct tgcagtagaa gacgcccggc tcgtagatct cgtccgactc cttgcccttg
     4921 gggtagagca tgcgccggcc gaagtcgcgg ttgatggcgg cgccaccgac cagcaccggg
     4981 tagtccagct cgcggtcgtg cagctcgccc acggcgatcg gcatctgctt cgaggtcgag
     5041 accagcagcg cggacaggcc gatggcgtcg gccttgtgct cctcggccgc gctcaggatc
     5101 gtgtcgaccg ggacctgctt gcccaggtcg acgaccgtgt agccgttgtt ggtcatgatc
     5161 gtgttgacca gcgacttgcc gatgtcgtgc acgtcgccga acacggtggc caagaccacg
     5221 gtgcccttgg tgtggccctc catcttctcg aggtagttct cgagctgggc cacggcgcgc
     5281 ttcatgacct cggcgctctg gagcacgaac ggcaggatca gctcaccgga gccgaacttg
     5341 tcgcccacct ccttcatggc gggcagcagc acggtgttca gggtggggac ggcgccgatc
     5401 ttctcgttcg acttgtcgat ccactcctcg acgccctcct tcttgcggcg caggatgtgc
     5461 cagtgcagcg cctcctcggg ctccatgccc tcggtggggt tctctgcctc ggcctcggcc
     5521 tcgtcgccct gggactcgaa gtgcccgatg aacttctcga gcgcgtcctc ggactcgttg
     5581 aagaccagcg cgtcggtgag cttgcgctcc tcctcggaga tctcgccgta gggcgtgacg
     5641 tccgcggggt gcaccatcgc caggtccagg ccggcctcga cgcagtggtg caggaagacc
     5701 gagttcagga tcttgcgggc cttcggcgag acgccgaacg acacgttcga gacgcccagc
     5761 gaggtcttga ccttgggcag cgccttcttg agctcgcgga tgccctcgat ggtctcgacg
     5821 gccgacgggc gccactcctc gtcaccggtg gtgagcgtga aggtcaggac gtcgaagatc
     5881 agcgactcct cgtccaggcc gtgctcgtcc acgaccatgt cgcggatgcg ctgcgcgatc
     5941 tcgaccttgc ggtcgcgcgt cttggccatg ccctcttcgt cgatggtcag cgcgatcagc
     6001 gcggcgccgt gggccttgac cagcggcacg accttgtcga gcttgtcgcg gccggcctcc
     6061 aggttcacgg agttcacgat cgcgcggccc ggcgtctgct cgagggccag cttcatgacc
     6121 tcgggctcgg tcgagtcgat ctgcagcggc gcgggcagcg acagcgacac gatcttcgcc
     6181 acctggcgca tctgctcgtc ctcgtcctcg cgctcggtga gcgcgacgca caggtccagc
     6241 acgtgcgcgc cgccctcgac ctggctggtg gccaggttca gcagcgagtc gtagtcgtcg
     6301 gccaggagca tctccttggc cttgcgcgag ccctgggagt tcacgcgctc gcccacgatc
     6361 gtgggggcgg gctcctgggc cagcggggtg gcgctcatca tggagctgac cttcagctcg
     6421 ccgcgctcgg gacgcggctt gacctgcttg tccttcacgc ggtcggcgat ggccttgatg
     6481 tggtcgggcg tggtgccgca gcagccaccc acgatcgaga cgccgttctt ctcgacgaac
     6541 tcggccagca cgtcggccag cggctcgggc tcggccggga agatcgtctc gccgtcgggc
     6601 ccctggtagg ggatgccggc gttggggatg cagtgcaccg gcaccgggca gttctcgccc
     6661 aggtagcgga tggcgtcgcg catgtcgtcc gggccggtcg agcagttgag cccgatcacg
     6721 tccacgtcga gcgcgtcgag ggtggtcagc accgcctcga tgtcggtgcc cagcagcatc
     6781 ttgccgccgt tgggcagcag cgagaccgag cactggatcg gcacttcacg gcccgtgctc
     6841 tccatcgcgg cgcgggcgcc gaagacggcg gccttgacct ccaggatgtc ctgggcggtc
     6901 tcgatgatga tcaggtcgac gccgccgttg atcaggccct cggcctgctc ggtgaacacc
     6961 tcgaccagct cggagaagag gatcttgccc agcgtggggt cgtcggacgc gggcaggtgg
     7021 cccgtcgggc cgatcgagcc cgcgacgaag ttgtcctcgc ccgcggcctt gcgggcgatc
     7081 tcggcggcct tctggttgat ctcgaccgtg tggtcctcga ggccccactc ctccagcttg
     7141 atccggctgg cctggaaggt gtcggtctcc agcacgtcgg cgccggcctc gatcatggac
     7201 gtgtggacgc cctcgatcac gtcggggcgc tgcaggacca gtgcctcgtg gcacttgccc
     7261 tggaggccca tgtagtcctc ctgggtcagg tcgaactgct cgagggtcgc gcccatgccg
     7321 ccgtcaaaga cgacgacgcg ctcgttcacg gtcttgaggt agtcgcgggc cattccccac
     7381 actctactac aaccttgaca cgactgtgtg aaggttggaa cgccccgctc agccgccgga
     7441 ttccacgccc tgctcgagca ggcccagctc gccggcgcgg ccggcggcct cggtccgggt
     7501 gcggcaccgg agcttggtca ggatgctccg gacgtgcatg tcgacggtgc gaacgctgag
     7561 caccaggcgc tcggcgatct cgcggttggt cagcccgccg gccacgagcc ggacgacctc
     7621 gacctcgcgc cgtgagagcc ccccgtgctc gtgggcggcg gcggcccgcc tgcccaggag
     7681 gtcctcgacc gacgccccct gggccgccac ctcggccgcg acgtcgcccg ccagcacggc
     7741 ggcacccagc atggtggcga cgcggtgggc ctcggccagc cgctccaggg cggcctcggg
     7801 ctcgccggcg gcggccagca ccacgcccgc gcggagcagg acctgggccc gctcgaaggg
     7861 gatgtccagc ccggcgtgca gctcgatcgc ccgcgtcagc tgctcggcgg ccgcgaccgg
     7921 gtcgccctcg gccagcgccg cctcgccgag ggcatgcgcc agcgccgcca gcgcgtcggg
     7981 gtagcccgcc gagccggcga tctgggacag ggcctcggcg cacgcgcggg cgtccgcggc
     8041 ggcgtcgtgc cgggcgaacc acgccgccga ccagcgcagg ccccagaccg cgtagtggtg
     8101 gtcctcgctg cggctccagc gctccaggac cagccggcag agctcgcgga cgcggtcgtc
     8161 gtcgccctcc tcggcggcca gccacgccag cgcggcggcg ctgtcgcact gcatcgagac
     8221 cacgtccagg cgggtggcgg tctccaggca gcgcgtgagc agcggccgcg cctgctcggg
     8281 gcggccgcgc cagaggtgca ccgcgcccag cacgccgtcg gccaccaggg tgtcgtccgg
     8341 cccggagtcc ggggtcagca ggccgtcgca cagctcctcg acctggtccc agtcgcccag
     8401 ctcgcgcagc acgtacgcca tgcagctgag gcaggtgtgc tccagccctc cgccgggcgc
     8461 gcgctcgcag agggtgatgg cggtgccgag cgactcccgc gccttcgcgt agtcgccggc
     8521 cacctcgtgg gcggtgccca ggcgctggta gacctcggcc gcctccaggg tcagcccccg
     8581 gtccagtgcc agcgacaggc cgccgcgcag gatctcgatg ccctccgcga acgacccctg
     8641 cttcacgcgg gcgacgccct ccaggcccat cgcccgcgcc cgcaggtcgg cccggtcgca
     8701 gcgggcggcc tcctcggcgg ccgtccgcgc cgtggccgcc gcctcggtgt gccggcccgc
     8761 ggcctgcagg tagctgcccg cgaccaggcg ctcgacggcg gcctcgccgg gcaggtcgtt
     8821 ggccgcgaac gcgtcggccg cgaccctgcg cgccgcgagc gccaggtcgc gcttgccctg
     8881 ga
//
LOCUS       DASUPW010000080         5502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1679131, whole genome shotgun sequence.
ACCESSION   DASUPW010000080 DASUPW010000000
VERSION     DASUPW010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5502
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1679131"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VFY44_01850"
     CDS             <1..327
                     /locus_tag="VFY44_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909204.1"
                     /translation="PGAGAAFMIATVESAVSAVSEAAEPIFGVEQSILGTPVAALLGS
                     PMGQDRLLRTVTQAATRAREPEVMPVRGLTPNAEGLGMMAARISTCGRPRAALITVEP
                     SDFGRR"
     gene            502..1422
                     /locus_tag="VFY44_01855"
     CDS             502..1422
                     /locus_tag="VFY44_01855"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX5909205.1"
                     /translation="MSGIRTNAAAELLGVSPNTLRSWERRFGYPKPRRTSGGHRQYEL
                     VELEALRRALLETHNISSAIEVARQRGEGPSSPSRLLDAFDHFDEMAADRVMEESLAV
                     RSVDRTVEEVLLPAIEMAEGRESREAEWELACRWATGWLYAARRVVPPASRAQGVILF
                     DSSERLDHESLHVQALELAVRRAGFRSLLLSFDLPPERVARAMRAVDPTAFVFCGGEA
                     TLEVLGRLVYTVRQVGSAAPVFEYRESMPVTGDHAIPSLGSMPTEAAERLKQYVDTGR
                     LEQVVVVAEGGGASEEDSTALPGVRLAANR"
     gene            1456..1890
                     /locus_tag="VFY44_01860"
     CDS             1456..1890
                     /locus_tag="VFY44_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5909206.1"
                     /translation="MADLQLGDPLAHRALEALLQAETSVRRVLASELEREGVSAAGFS
                     ALVVLTTAGGELGLRTLRRRLDWSKANATEVTLTLESRGLVTRRRLTTDRRATAVHLT
                     PDGQALVERLFPGHADRVSTAFSRLDEDEKRSLALICRKLAA"
     gene            complement(1887..3059)
                     /locus_tag="VFY44_01865"
     CDS             complement(1887..3059)
                     /locus_tag="VFY44_01865"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX5909207.1"
                     /translation="MEGAPPALRRLLETARGVAGRSRTVQAAVARLEGVVLPEHLPER
                     VRDDVLAAIERARADTSWRLPVSDVERELDGQLDSLEEAPDAVEAHAQTHRAVIDGAA
                     VRVKVGRPGLAVSARSDLSLLDLMAAPARTAFPALDVGPLIREVRDRAVAELDFEHEA
                     ALQRRVGRALRPVEGVDVSRVLLDRCSECVVVAEEAPGTALDDPAALEGVDRAAVARR
                     LVTVFCGAPGALGMVLADARAAETYVQGDGTVTLQWVGAGREVDPRRLEAAVGAAQAL
                     RIDDAEAFALALKALGLLEHDDAVAAHALLRDVLGPLVAGPALLDAEALRGAADRALD
                     HGRDLLGLATRVTAQPEDLWALRALGHTVSLLARLEVEEDWLVAGVTAVNGGWEPA"
     gene            3103..>5502
                     /gene="ileS"
                     /locus_tag="VFY44_01870"
     CDS             3103..>5502
                     /gene="ileS"
                     /locus_tag="VFY44_01870"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX5909208.1"
                     /translation="MPRYAPVDPQQSFPALEQEILERWRERDIFGESVRRREGAEPYV
                     FYEGPPTANGRPGSHHVLSRVFKDVFPRYRTMRGRLVHRKGGWDTHGLPVELEIEKQL
                     GFKTKDDIEAYGVAEFNAKCRESVLKYIDEWHQLTERIGFWIDTDDAYYTMDDDYIES
                     VWWSLKQVWDKGLLYEGYKVVPYCPRCGTALSSHEVSLGYKDVVDTAAFVRFPVVGQE
                     DVSFVGWTTTPWTLLSNAALAVGPDITYVRVAHEGEQLIVAEALAEKVFGEGVQTSDP
                     VPGSDLVGLAYEPPFSFVTDFGEKGHTVLPADFVTTEDGTGVVHTALAFGEDDFRLGE
                     EQGLTLQNPVRRDGTFDDRMGPYAGRKVKETDADIVEELRADGKLLRAEDYEHSYPHC
                     WRCDTALLYYAKSSWYVATTKVKDQLLASNEAVTWYPEHIRTGRMGRWLENNVDWALS
                     RERYWGTPLPIWRADDGEVVCVGSRAELAELGAEVPDDLHRPYVDDATFERDGKTFRR
                     VPDLIDVWWDSGAMPFAQWHAPFENEDTFEARYPADYICEALDQTRGWFYSLLAVSTL
                     LFGRASFETVLCLGLITDPQGQKMSKSRGNAVAPWDVLDTHGADAFRWYYFTSKQPWD
                     GYRFSVETIGESVRQYLKTLWNTYGFFALYANANDVEPASLDEVELTDLDRWALSRLQ
                     SLSATCIDRLDDYDTTSAGRAIQAYVDELSNWYVRRSRRRFWDGDPAAFATLRECLLG
                     VAKLTAPLTPFVADAIYEGLDAGEPSVHLCDYPEPDPALRDERLEWQMGVAREAVGLG
                     RE"
BASE COUNT          768 a         2083 c         1919 g          732 t
ORIGIN      
        1 ccgggagcgg gagccgcgtt catgatcgcc accgtggagt ccgcggtcag cgcggtcagc
       61 gaggccgcgg agcccatctt cggcgtcgag cagagcatcc tcggcacgcc ggtggccgcc
      121 ctgctgggca gcccgatggg ccaggaccgg ctgctgcgca ccgtcaccca ggccgccacc
      181 cgggcgcgcg agcccgaggt catgccggtt cgcgggctca cgccgaacgc cgaggggctg
      241 ggcatgatgg ccgctcggat ctcgacctgc gggcgcccgc gcgccgcgct gatcacggtc
      301 gagcccagcg atttcggtcg ccggtagctg ccggatcgcc tgcaaaaacc cctgcactgt
      361 ttgcacgaaa gcggtgcaac cgtcacaaac ctctcaagtg ccgcgcagct tcggccgaaa
      421 agctgtgcag ccaggggcct ctttctcgtg tgcgctggct tcgctgtaga accaacccac
      481 cgtgagtgcc aaggagacca agtgagcggg atcaggacaa atgctgcggc agagttgctc
      541 ggggtcagcc ccaacacgct gcgcagctgg gagcgccgct tcggctaccc caagccccgc
      601 cgcacctccg gcggccaccg ccagtacgag ctcgtcgagc tcgaggcgct gcgccgcgcc
      661 ctgctggaga cgcacaacat ctcctcggcg atcgaggtcg cccgccagcg gggcgagggc
      721 cccagctcgc ccagccgcct gctggacgcg ttcgaccact tcgacgagat ggccgccgac
      781 cgcgtgatgg aggagagcct ggccgtccgc tcggtggacc gcaccgtcga ggaggtactg
      841 ctcccggcga tcgagatggc cgagggtcgc gagtcgcgtg aggccgagtg ggagctggcc
      901 tgccgctggg ccaccggatg gctctacgcc gcccgccgcg tcgtcccacc cgcctcccgc
      961 gcgcagggag tgatcctctt cgactcctcc gagcgcctgg accacgagtc gctgcacgtg
     1021 caggccctgg agctggccgt gcgccgcgcc ggcttccgct cgctgctgct gagcttcgac
     1081 ctcccgcccg agcgcgtcgc ccgcgcgatg cgcgccgtcg acccgaccgc gttcgtcttc
     1141 tgcggcggcg aggccaccct cgaggtgctg ggccggctgg tctacaccgt tcgccaggtc
     1201 ggctccgccg ccccggtgtt cgagtaccgc gagtcgatgc ccgtcaccgg cgaccacgcc
     1261 atcccctcgc tgggctcgat gcccaccgag gcggccgagc ggctcaagca gtacgtggac
     1321 accggccgcc tggagcaggt ggtcgtggtg gccgagggcg gcggtgcctc cgaggaggac
     1381 agcaccgccc tgccgggcgt ccgcctcgcc gcgaaccgct agcgccgtcc ggagccgcgc
     1441 atagcctggc gtctcgtggc agacctgcag cttggcgacc cgctcgccca ccgtgccctg
     1501 gaggcgttgc tccaggccga gacctccgtc cgccgcgtcc tggcttccga gctcgagcgc
     1561 gagggcgtgt ccgcggccgg cttctcggcc ctggtcgtcc tgaccaccgc cggaggcgag
     1621 ctcggccttc gcaccctccg ccggcgcctg gactggtcca aggccaacgc caccgaggtc
     1681 acgctgacgc tggagtcgcg cggcctggtc acgcgccggc gcctgaccac ggaccgccgc
     1741 gccaccgccg tgcacctgac gccggacggc caggcgctgg tcgagcgcct gttcccgggc
     1801 cacgccgacc gcgtcagcac cgccttctcc cggctcgatg aggacgagaa gcgctcgctc
     1861 gccctgatct gccgcaagct cgcggcctag gccggctccc agccgccgtt cacggcggtc
     1921 acgccggcca ccagccagtc ctcctcgacc tccagccgcg ccagcagcga gacggtgtgg
     1981 cccagcgcgc gcagggccca caggtcctcg ggctgggcgg tgacgcgcgt ggccaggccc
     2041 agcaggtcgc gaccgtggtc cagtgcccgg tcggcggcgc cgcgtagcgc ctcggcgtcc
     2101 agcagcgccg gacccgcgac cagcgggccc aggacgtcgc gcagcagcgc gtgggcggcc
     2161 accgcgtcgt cgtgctcgag cagccccagc gccttgagcg cgagggcgaa cgcctccgcg
     2221 tcgtcgatgc gcagcgcctg cgcggcgccc acggccgcct ccaggcggcg cgggtcgacc
     2281 tcgcggccgg cgccgaccca ctggagcgtc accgtgccgt cgccctgcac gtaggtctcg
     2341 gcggcgcgcg cgtcggccag caccatgccc agcgcgcccg gcgcgccgca gaagaccgtg
     2401 accagccggc gggccaccgc ggcccggtcg acgccctcca gcgcggcggg gtcgtccagg
     2461 gcggtccccg gcgcctcctc ggccaccacg acgcactccg agcagcggtc caggagcacc
     2521 cgcgacacgt ccacgccctc caccgggcgc agcgcccggc cgacgcggcg ctggagcgcg
     2581 gcctcgtgct cgaagtccag ctcggcgacg gcccggtcgc ggacctcgcg gatcagcggg
     2641 cccacgtcca gcgccgggaa cgccgtgcgc gcgggcgcgg ccatcaggtc cagcagcgac
     2701 aggtccgagc gcgcggacac ggccaggccc gggcggccga ccttcacccg caccgcggcc
     2761 ccgtcgatca ccgcgcggtg ggtctgggcg tgggcctcga cggcgtcggg cgcctcctcc
     2821 agggagtcca gctgaccatc gagctcgcgc tcgacgtcgg acaccggcag gcgccaggac
     2881 gtgtcggccc gggcgcgctc gatcgccgca agcacgtcgt cccgcacgcg ctcggggagg
     2941 tgctcgggca ggaccacgcc ctccagccgc gccacggcgg cctggaccgt gcgcgagcgg
     3001 cccgccaccc cgcgggcggt ctcgagcaac cgccgtagag ccggaggggc gccttccacc
     3061 ccgctgacgc tagacggtca tccatagact cgtcgctccc cgatgccccg ctacgcgccc
     3121 gtcgatccgc agcagtcctt ccccgccctg gagcaggaga tcctggagcg ctggcgcgag
     3181 cgcgacatct tcggcgagtc ggtccgccgg cgcgagggcg cggagcccta cgtcttctac
     3241 gagggcccgc cgaccgccaa cggccgcccc ggctcgcacc acgtgctcag ccgcgtgttc
     3301 aaggacgtct tcccgcgcta ccggaccatg cgcgggcgcc tggtgcaccg caagggcggc
     3361 tgggacaccc acggcctgcc cgtcgagctg gagatcgaga agcagctggg cttcaagacc
     3421 aaggacgaca tcgaggccta cggcgtggcc gagttcaacg ccaagtgccg cgagtcggtc
     3481 ctgaagtaca tcgacgagtg gcaccagctg accgagcgga tcggcttctg gatcgacacc
     3541 gacgacgcct actacacgat ggacgacgac tacatcgagt cggtctggtg gtcgctcaag
     3601 caggtgtggg acaagggcct gctgtacgag ggctacaagg tcgtgcccta ctgcccccgc
     3661 tgcggcaccg cgttgtcctc gcacgaggtg tcgctgggct acaaggacgt ggtcgacacc
     3721 gccgcgttcg tccgcttccc ggtcgtgggc caggaggacg tctcgttcgt gggctggacc
     3781 acgaccccgt ggacgctgct ctccaacgcc gccctggcgg tcggccccga catcacctac
     3841 gtgcgcgtcg cccacgaggg ggagcagctg atcgtcgccg aggcgctggc cgagaaggtc
     3901 ttcggcgagg gcgtacagac gtcggacccc gtgcccgggt cggacctcgt gggcctggcc
     3961 tacgagccgc cgttctcgtt cgtgaccgac ttcggcgaga agggccacac ggtcctgccg
     4021 gccgacttcg tgaccaccga ggacggcacc ggcgtggtgc acacggccct ggctttcggc
     4081 gaggacgact tccgcctggg cgaggagcag gggctgacgc tgcagaaccc ggtccgccgc
     4141 gacggcacct tcgacgaccg catgggcccc tacgccggcc gcaaggtcaa ggagaccgac
     4201 gccgacatcg tcgaggagct gcgcgccgac gggaagctgc tgcgcgccga ggactacgag
     4261 cacagctacc cgcactgctg gcgctgtgac accgcgctcc tctactacgc caagtcctcc
     4321 tggtacgtgg ccaccacgaa ggtcaaggac cagctgctgg cctccaacga ggcggtcacc
     4381 tggtacccgg agcacatccg gaccgggcgc atggggcgct ggctggagaa caacgtcgac
     4441 tgggcgctca gccgcgagcg ctactggggc acgccgctgc ccatctggcg cgccgacgac
     4501 ggcgaggtcg tctgcgtcgg ctcgcgcgcc gagctggccg agctaggggc cgaggtcccg
     4561 gacgacctgc accgccccta cgtggacgac gccaccttcg agcgcgacgg caagacgttc
     4621 cggcgcgtgc ccgacctgat cgacgtgtgg tgggactcgg gcgcgatgcc gttcgcccag
     4681 tggcacgcgc cgttcgagaa cgaggacacg ttcgaggccc gctacccggc cgactacatc
     4741 tgcgaggcgc tggaccagac ccgcggctgg ttctactcgc tgctggcggt gtcgacgctg
     4801 ctgttcggcc gcgcctcctt cgagacggtg ctgtgcctgg gcctgatcac ggacccccag
     4861 ggccagaaga tgtccaagtc caggggcaac gcggtggcgc cctgggacgt gctggacacc
     4921 cacggcgccg atgcgttccg ctggtactac ttcacctcca agcagccctg ggacggctac
     4981 cggttctcgg tcgagaccat cggcgagtcc gtccgccagt acctgaagac gctgtggaac
     5041 acgtacggct tcttcgctct gtacgcgaac gccaacgacg tcgagcccgc gtcgctggac
     5101 gaggtcgagc tgaccgacct ggaccggtgg gcgctgtcgc ggctgcagtc gctcagcgcc
     5161 acgtgcatcg accgcctcga cgactacgac acgacctcgg ccggacgggc catccaggcc
     5221 tacgtcgacg agctctccaa ctggtacgtg cgccgctcgc ggcggcggtt ctgggacggc
     5281 gaccccgcgg ccttcgcgac gctgcgcgag tgcctgctgg gcgtggccaa gctcacggcc
     5341 ccgctgacgc cgttcgtggc cgacgcgatc tacgagggtc tggacgccgg cgagccgtcg
     5401 gtgcacctgt gcgactaccc cgagcccgac cccgccctgc gcgacgagcg cctggagtgg
     5461 cagatgggcg tggcccgcga ggccgtcggc ctcggccgcg ag
//
LOCUS       DASUPW010000081         3574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1956892, whole genome shotgun sequence.
ACCESSION   DASUPW010000081 DASUPW010000000
VERSION     DASUPW010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1956892"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1565)
                     /locus_tag="VFY44_01875"
     CDS             complement(<1..1565)
                     /locus_tag="VFY44_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX5909209.1"
                     /translation="MRRSSLLCAAVAIAAAVCAAPAAAAPTGPLSQKGRWITDAHGRV
                     AIMHGVNMVSKRPPYAPVTTGFGEDDARFLRREGFDTVRLGLVYAGVEPSPGRYSDAY
                     LEQIQQTADALSKAGVFYQLDFHQDLYNERFEGEGWPDWAVQDDGLPAVPKNGFPANY
                     LLMPALSRAFDHFWANDPGPGGVGLQDRYAEAWRQVAARFRGQQHNMGYDLLNEPWPG
                     TGWQACANTEGCPAFDRDKLTPFSERVYRQIRRADPDGIVWYEPNVLFNNGPKSHHGA
                     IGRKTGLSFHVYCLGGGATPASDPVDAAAREARCDPLERLPFVNAEDQARRTDSTQLL
                     SEFGATDDLDQIRRVVRDAEEHMISWQYWHYCPCADPTTTGSGSTQALVLDPARPPTG
                     DNVVARKLAVLSRAYPQAVAGTPERYRFDADRKRFDMTYSTARVGGGGFARRADTQVF
                     VPSRHFTRGYDVTVSGGEAISAPSSPHLVVRNCNGRARVSIAVSAGSGARRADCRAPR
                     YGPARFRLKVSPRR"
     gene            complement(1731..2210)
                     /gene="greA"
                     /locus_tag="VFY44_01880"
     CDS             complement(1731..2210)
                     /gene="greA"
                     /locus_tag="VFY44_01880"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX5909210.1"
                     /translation="MPKETLLTPQGLEDLKAKIDHLSTARRREVADRIREARDFGDIA
                     ENSEYDDAKNEQAMLEKQISDLEDKLRNSRVIDESEVTSDAVSVGVTVHVKDQKTDKS
                     TKFKIVGSAEASPAESKLSNESPVGKALMGHKRGETVSVPVPKGPARKLKITKIEAA"
     gene            complement(2292..3287)
                     /locus_tag="VFY44_01885"
     CDS             complement(2292..3287)
                     /locus_tag="VFY44_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEX5909211.1"
                     /translation="MSRALTDPWELAGHTLPNRLVLAPLAGIGNWFVRLQAKRYGAGF
                     VVSEMVSSFGLKHGNERTLREFLRIHPDEHPVSMQLFGHDADVMREASAVVAEAGADI
                     VDLNMGCPVRKVCKTGAGAALLDDPDKAVAIARAAGEGSGLPVTVKLRPGQRPGDRAG
                     VDLARRLVDEAGVAAIAFHPRHASQQHKGKADYDLAAELVEALDVPVIVSGGLLSDEK
                     AAVALERSGAAAVMLARGSLGNPWRFERLLGRREGEPTSAEVLDELRWVIDRAEEHLG
                     PQRAGRYLRKHYAWYAERLDLDKRVRHELVTAPGTDAVRAILSRIERGDAALQPA"
     gene            complement(3284..>3574)
                     /locus_tag="VFY44_01890"
     CDS             complement(3284..>3574)
                     /locus_tag="VFY44_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909212.1"
                     /translation="MLALPLLFVGIVARFPAGLAVYWITTSLWTLGQQVVMWRLQPDM
                     APQTMGGAMAMETGGTLEPADLGGGGNGSGATARKPPPQRTRKKKKAHGRRR"
BASE COUNT          442 a         1257 c         1344 g          531 t
ORIGIN      
        1 cgccgcgggc tgaccttcag ccggaaccgc gccggcccgt accggggcgc gcggcagtcg
       61 gcgcggcgcg cgcccgaccc cgcgctcacc gcgatcgaga ccctggcccg gccgttgcag
      121 ttgcgcacga ccaggtgcgg cgagctgggc gccgagatgg cctcgccgcc gctgacggtc
      181 acgtcgtacc cgcgcgtgaa gtggcggctg ggtacgaaca cctgcgtgtc ggcgcggcgg
      241 gcgaagccgc ccccgccgac acgggcggtg gagtaggtca tgtcgaagcg cttgcggtcg
      301 gcgtcgaagc ggtagcgctc gggcgtcccg gccaccgcct gcgggtaggc gcggctgagc
      361 acggccagct tgcgggccac cacgttgtcg cccgtcggcg gccgggccgg gtccagcacc
      421 agcgcctggg tgctgcccga gccggtggtg gtggggtcgg cgcacgggca gtagtgccag
      481 tactgccagc tgatcatgtg ctcctccgcg tcgcgcacca cccgccggat ctggtccagg
      541 tcgtcggtgg cgccgaactc gctgagcagc tgggtgctgt cggtgcggcg ggcctggtcc
      601 tcggcgttca cgaacggcag ccgctccagt ggatcgcagc gcgcctcccg cgcggcggcg
      661 tccaccgggt cggacgccgg cgtggccccg ccgccgaggc agtagacgtg gaagctcagg
      721 ccggtcttgc ggccgatggc gccgtggtgg gacttcggcc cgttgttgaa caggacgttg
      781 ggctcgtacc agacgatccc gtccgggtcg gcgcggcgga tctggcggta cacgcgctcc
      841 gagaacgggg tgagcttgtc gcggtcgaac gccgggcagc cctcggtgtt ggcgcacgcc
      901 tgccagccgg tgccgggcca cggctcgttc agcaggtcgt agcccatgtt gtgctgctgg
      961 ccgcggaagc gcgcggccac ctggcgccac gcctcggcgt agcggtcctg cagccccacg
     1021 cccccggggc ccgggtcgtt ggcccagaag tggtcgaagg cgcggctgag ggcgggcatc
     1081 agcaggtagt tggcggggaa cccgttcttg ggcaccgccg ggaggccgtc gtcctgcacc
     1141 gcccagtcgg gccagccctc gccctcgaag cgctcgttgt agaggtcctg gtggaagtcg
     1201 agctggtaga agacccccgc cttcgagagc gcgtcggcgg tctgctggat ctgctcgagg
     1261 taggcgtccg agtagcgccc cggggagggc tccacgccgg cgtagacaag cccgagccgg
     1321 accgtgtcga agccctcacg gcgcaggaag cgcgcgtcgt cctcgccgaa gccggtggtc
     1381 accggcgcat acggcgggcg cttggagacc atgttgaccc cgtgcatgat ggccacgcgc
     1441 ccgtgggcgt cggtgatcca gcggcccttc tgcgacagcg ggccggtggg cgcggcggcg
     1501 gcgggggccg cgcagacggc ggcggcgatc gccacggccg cacacagcag gctgcttcgc
     1561 ctcacggcgc caacctaacg cggcgacggg cgccgcgcga aggtcaggct tgcctgatca
     1621 cggctggcga ggccgtgggc ggggctgcca aggacgcagg gaggtcgtag tgcttcgcac
     1681 acgaccgacc gaggacgcgg gcaggcgcgt ccacgggccg ccaggcggga tcaggctgct
     1741 tcgatcttgg tgatcttgag cttgcgcgcc gggcccttgg ggaccgggac ggagaccgtc
     1801 tcgccgcgct tgtggcccat cagggccttg ccgacggggg actcgttcga gagcttgctc
     1861 tcggccggag aggcctcggc ggagccgacg atcttgaact tggtcgactt gtcggtcttc
     1921 tggtccttga cgtggaccgt cacgccgacc gagaccgcgt cgctggtgac ctcggactcg
     1981 tcgatcacgc gggagttgcg cagcttgtcc tcgaggtccg agatctgctt ctcgagcatg
     2041 gcctgctcgt tcttggcgtc gtcgtactcg gagttctcgg cgatgtcgcc gaagtcgcgc
     2101 gcctcgcgga tgcggtcggc cacctcgcgc cggcgggcgg tcgagaggtg atcgatcttg
     2161 gccttgaggt cttcaaggcc ttgtggtgtg agcagcgtct ctttcggcat gcgtatctcc
     2221 ctggatcccg gacccgacgc ccgggagagc gcggcagtct agcaaccgga atgcccgatt
     2281 ccggcggatc gtcaggcggg ttgcagcgcg gcgtcgccgc gctcgatccg gctcaggatc
     2341 gcgcgcaccg cgtcggtgcc cggcgcggtc accagctcgt gccgtacccg cttgtccagg
     2401 tccaggcgct cggcgtacca cgcgtagtgc ttgcgcaggt agcgcccggc gcgctggggc
     2461 cccagatgct cctcggcgcg gtcgatcacc cagcgcagct cgtccagcac ctcggcggag
     2521 gtgggctcgc cctcgcggcg gcccagcagg cgctcgaacc gccacgggtt gcccagcgag
     2581 ccgcgcgcga gcatcacggc cgcggccccg ctgcgctcca gcgccaccgc ggccttctcg
     2641 tcggaaagca ggcctcccga gacgatcacg ggcacgtcca gggcctcgac cagctcggcc
     2701 gcgaggtcgt agtcggcctt gcccttgtgc tgctgggacg cgtgccgggg gtggaaggcg
     2761 atggcggcca cgccggcctc gtcgaccagc cgccgcgcca ggtccacgcc ggcgcgatcg
     2821 ccggggcgct ggcccgggcg cagcttcacg gtcacgggca ggccgctgcc ctcgcccgcc
     2881 gccctggcga tggccaccgc cttgtcgggg tcgtccagca gcgccgcccc cgcgccggtc
     2941 ttgcacacct tgcgcacggg gcagcccatg ttcaggtcga cgatgtcggc gcccgcctcg
     3001 gcaaccaccg cggaggcctc ccgcatgacg tcggcgtcgt ggccgaacag ctgcatcgag
     3061 accgggtgct cgtcgggatg gatgcgcagg aactcgcgca gggtgcgctc gttgccgtgc
     3121 ttgaggccga agctcgagac catctccgag accacgaagc cggcgccgta gcgcttggcc
     3181 tgcaggcgca cgaaccagtt gccgatgccg gcaagggggg ccaggaccag gcggttgggc
     3241 agggtgtgcc cggccagctc ccaggggtcg gtcagcgcgc ggctcatcgc cgccggccgt
     3301 gggccttctt cttcttgcgc gtgcgctgtg gcggcggctt gcgggcggtg gcgccgctgc
     3361 cgttcccgcc gcccccgagg tcggccggct ccagcgtgcc gccggtctcc atggccatcg
     3421 cgccacccat ggtctggggg gccatgtcgg gctggagccg ccacatcacg acctgctggc
     3481 ccagcgtcca caggctggtg gtgatccagt acacggccag gccggcgggg aagcgggcca
     3541 cgatgcccac gaacagcagc ggcagggcca gcat
//
LOCUS       DASUPW010000082         3018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_710023, whole genome shotgun sequence.
ACCESSION   DASUPW010000082 DASUPW010000000
VERSION     DASUPW010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710023"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VFY44_01895"
     CDS             <1..362
                     /locus_tag="VFY44_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX5909213.1"
                     /translation="YSRLGALLDPLTDRALVLAGAIVCWNFELLPRWALAVLAARELF
                     MLILTQVGLRKGMDLEVNMIGRWAVWPIMFAIFLTLAVSTPIAEASLYLGLALTLWAT
                     AIYVQDGLRFLKASSST"
     gene            385..645
                     /locus_tag="VFY44_01900"
     CDS             385..645
                     /locus_tag="VFY44_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909214.1"
                     /translation="MDTFPDLGALSDDELKQLIQQYTEEEQEVSYQRRILHGKIDILR
                     AELVNRLRKKHEDGESSLSGADVQQLTDILSGKSSPEGGGEA"
     gene            656..1117
                     /locus_tag="VFY44_01905"
     CDS             656..1117
                     /locus_tag="VFY44_01905"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5909215.1"
                     /translation="MHCPECGFVNTEGANYCQKCGAFLAEGAEAEGDTTAQYQLEESG
                     ELRPVDVNEVEASGATLAIRSGGGRAGEVFALDGERMTIGRSPDAEVFLDDVTVSRNH
                     ALIVRRRDGLYVDDLGSLNGTYVNRHRIESHRLSDGDELQVGKYKLTFLER"
     gene            1114..1851
                     /locus_tag="VFY44_01910"
     CDS             1114..1851
                     /locus_tag="VFY44_01910"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX5909216.1"
                     /translation="MSSPPDTDQAPTGKALTIGAVCKALEREFPDISISKIRYLEDQK
                     LISPRRTQGGYRLFAPTDVTRLRTILRLQRDEFLPLRVIRQELASGRVQDESPQNGSG
                     GAPDPRGMRRATVAMKSGGSLYPLDQVLEETGADASLVKELEDYGVIKAEKYDGEKLY
                     DDTEREIIRAVTELKRFGVGGRNLRVFRTSADREAALLEQILAPALRSRNVERRKEAM
                     QALENLAATASYLKHLLLIRDLRKIAK"
     gene            1848..2381
                     /locus_tag="VFY44_01915"
     CDS             1848..2381
                     /locus_tag="VFY44_01915"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002634.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX5909217.1"
                     /translation="MTAGDRVAELIRDIPDFPKPGIVFKDVTPVFADPEGLRAAVDCV
                     VGHATDVDATHIVSAEARGFVLGGAGASAAGLGFVLGRKPGKLPRDTVAVEYELEYGT
                     DVLEVHADAFPPGARVLVHDDLLATGGTASALCELVERAGAEVAGCSFLIELAFLNGR
                     DRLTGRRVESLVSFDSE"
     gene            2391..2840
                     /locus_tag="VFY44_01920"
     CDS             2391..2840
                     /locus_tag="VFY44_01920"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5909218.1"
                     /translation="MPVVSRSRTIPAAPERVWTEVADPEHLPRWWPGVDRVEDASRDA
                     WTAVLTSGKGKWLRADYTLLESEHPRRRRWRHEVEESPFERILSESVTELELEPVEEG
                     TRVTLTARLKPRGFSRFGGMQIRRATARQLSEALDGLETISSGWRST"
BASE COUNT          448 a         1045 c         1098 g          427 t
ORIGIN      
        1 agtacagccg cctgggggcc ctcctggacc cgctgaccga ccgcgccctg gtgctggcgg
       61 gggcgatcgt gtgctggaac ttcgagctgc tgccgcggtg ggcgctggcc gtgctggccg
      121 cgcgcgagct gttcatgctg atcctgaccc aggtcggcct gcgcaagggc atggacctcg
      181 aggtcaacat gatcggccgc tgggcggtgt ggccgatcat gttcgccata ttcctgacgc
      241 tggcggtctc cacgccgatc gccgaggcgt ccctgtacct cgggctggcc ctgacgctgt
      301 gggcgacggc gatctacgtg caagacggct tgcgcttcct caaagcctca agctccactt
      361 gacgcctcgg tctatactcg gcggatggac accttccctg acctcggggc tctctccgac
      421 gacgagctca agcagctgat ccagcagtac accgaggagg agcaggaggt gtcgtaccag
      481 cgccgcatcc tgcacgggaa gatcgacatc ctcagggccg agctggtgaa ccggctgcgc
      541 aagaagcacg aggacggcga gtcgtcgctg tccggtgccg acgtgcagca gctgaccgac
      601 atcctgtcgg gcaagagcag ccctgagggt ggcggcgagg cctagggaag gcctcatgca
      661 ctgccccgaa tgcgggttcg tcaacaccga gggggcgaac tactgccaga agtgcggcgc
      721 cttcctggcc gagggcgccg aagccgaggg tgacaccacg gcccagtacc agctggagga
      781 gagcggcgag ctccggcccg tcgacgtcaa cgaggtcgag gcctcgggcg ccacgctggc
      841 catccgctcc ggcggtgggc gggcgggcga ggtcttcgcg ctcgacggcg agcgcatgac
      901 gatcgggcgc agccccgacg ccgaggtctt cctggacgac gtgaccgtgt cccgcaacca
      961 cgcgctgatc gtgcgccggc gcgacgggct ctacgtggac gacctgggct ccctgaacgg
     1021 cacctacgtg aaccgccacc ggatcgagtc ccaccggctc tccgacggcg acgagctcca
     1081 ggtgggcaag tacaagctga cgttcctcga gcgatgagca gccccccgga caccgaccag
     1141 gcgccgacgg gcaaggcgct gaccatcggc gcggtctgca aggcgctgga gcgcgagttc
     1201 cccgacatct cgatctcgaa gatccgctac ctggaggacc agaagctgat ctcgccgcgg
     1261 cgcacccagg gcggctaccg cctgttcgcg cccaccgacg tgacccgcct gcgcacgatc
     1321 ctgcgcctgc agcgcgacga gttcctgccc ctgcgggtga tccgccagga gctggcgtcc
     1381 gggcgcgtcc aggacgagag cccgcagaac ggctccggcg gcgcccccga cccgcgcggc
     1441 atgcgccgcg cgacggtggc catgaagtcg ggtggctcgc tgtacccgct cgatcaggtg
     1501 ctggaggaga cgggcgccga cgcctcgctg gtcaaggagc tcgaggacta cggggtgatc
     1561 aaggccgaga agtacgacgg cgagaagctc tacgacgaca ccgagcgtga gatcatccgc
     1621 gccgtcaccg agctcaagcg gttcggcgtc ggcgggcgca acctgcgcgt gttccgcacc
     1681 tcggccgacc gcgaggccgc cctgctggag cagatcctgg ccccggccct gcgctcgcgc
     1741 aacgtggagc ggcgcaagga ggccatgcag gcgctggaga acctggccgc caccgcgtcc
     1801 tacctcaagc acctgctgct gatccgggat ctgcggaaaa tcgccaagtg accgcaggtg
     1861 accgggtcgc ggagctgatc cgcgacatcc ccgacttccc caagccgggc atcgtgttca
     1921 aggacgtgac gcccgtgttc gccgaccccg aggggctgcg ggccgccgtg gactgcgtgg
     1981 tcgggcacgc caccgacgtg gacgcgacgc acatcgtctc ggcggaggcg cgcgggttcg
     2041 tgctgggcgg cgccggcgcg tccgcggccg gcctgggctt cgtgctgggc cgcaagccgg
     2101 gcaagctgcc ccgggacacc gtggcggtgg agtacgagct ggagtacggc accgacgtcc
     2161 tggaggtgca cgccgacgcc ttcccgcccg gcgcccgggt gctggtccac gacgacctgc
     2221 tggccaccgg tggcaccgcc agcgccctct gcgagctggt cgagcgggct ggggcggagg
     2281 tcgcgggatg ctcgttcctg atcgagctgg ccttcctgaa cggccgtgac cggctcaccg
     2341 gccgcagggt cgagagcctg gtctccttcg actcggaata gagtctcggc gtgcccgtcg
     2401 tgagccgctc ccggacgatt cccgccgcgc cggagcgcgt gtggaccgag gtggccgatc
     2461 ccgagcacct cccgcgctgg tggccggggg tggaccgggt cgaggatgcg tcgcgtgacg
     2521 cctggaccgc cgtgctgacg tctgggaagg gcaagtggct gcgggccgac tacacgctgc
     2581 tcgagtccga gcacccgcgc cggcgccgct ggcgccacga ggtcgaggag tcgccgttcg
     2641 agcggatcct gtccgagtcg gtgacggagc tggagctgga gcccgtcgag gagggcacgc
     2701 gcgtgacgct gaccgcgagg ctcaagccgc gtggcttctc gcgcttcggg gggatgcaga
     2761 tccgtcgcgc caccgcacgc cagctgtccg aggccctgga cggcctggag acgatctcga
     2821 gcggctggag aagcacgtga tgcgctggtg ggggtggggc gaggacgagc acgcctcgcc
     2881 gctgtccgag cgcgcccagg agagggtcgg cgccctgctg ggcgtggagc ccggccagcc
     2941 accggccgag cgtcgcggcc ccgagtccgt ggtccttccg tcgcccacct tgcagtcctc
     3001 cgcccgcgcg gccctgga
//
LOCUS       DASUPW010000083         3210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1541122, whole genome shotgun sequence.
ACCESSION   DASUPW010000083 DASUPW010000000
VERSION     DASUPW010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1541122"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VFY44_01925"
     CDS             complement(<1..363)
                     /locus_tag="VFY44_01925"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5909219.1"
                     /translation="MATNERFMPVAPEAVWDALADPGGYGYWVVGSKHIRDVDADWPA
                     AGSRFHHTVGFGPLTLDDHSEIVEAERPRLFRMRVKGRPAGTALVTLEMKPRPADGGT
                     LVRFSENPDGVFAWLSLNP"
     gene            complement(365..751)
                     /locus_tag="VFY44_01930"
     CDS             complement(365..751)
                     /locus_tag="VFY44_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909220.1"
                     /translation="MSNSGRKVAVGFAVLRIGYAVGLLAAPNRVARAWVGSAAESPGG
                     AVALRGLGARDLVLSVGVAASALSGRDARPWLAACAVSDAADLSATLLAPGSALPSRA
                     KPGAVALAGGFGALGAALAAHDDLAG"
     gene            814..1617
                     /locus_tag="VFY44_01935"
     CDS             814..1617
                     /locus_tag="VFY44_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909221.1"
                     /translation="MAAKKPKTGADALDEVYLGDFEGFVARRDALAKQLRADGDGDEA
                     DRVKALKKPSRTAWAVNQFAANGKKLRNDLLKAGGRLREASEGLVSGDADREAMLKAR
                     DGERAAVGAALKAIAALAAESGPELNAAATERVRQTLHAVALDDEVREQFEAARLTTD
                     HEASGLGAGGPATAAGGGAAKAKPATAAKKKGRDREAERKRRRRDELKAAEAEAAKRD
                     RDLKDADLELAEAKKAAKRAQGDLKRATTRQEKAKAAADDAAAKVQELG"
     gene            complement(1614..1979)
                     /locus_tag="VFY44_01940"
     CDS             complement(1614..1979)
                     /locus_tag="VFY44_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909222.1"
                     /translation="MKSDLMFRGDLHAPRAARMALDDLVHDLDGRLLADLRLVVTEMV
                     TSIIRTGNGAPLAVRVEILSPDHLRGEVVEDGDPVTGSASLTEEATAPILDELTTRWG
                     LGPHPRRMWFEIEAEAPAA"
     gene            complement(2072..2452)
                     /locus_tag="VFY44_01945"
     CDS             complement(2072..2452)
                     /locus_tag="VFY44_01945"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5909223.1"
                     /translation="MSGRLEAVVAGLELDPGHRVLEIGCGHGVAATIVCEQLDGGTYT
                     AIDRSPKMIAAAERRNRAHVRAGTAEFLTGALEEVDLGSRRFDRVFAVRVGLFGREPD
                     RARALASRWLAPGGTLQVVLDPPG"
     gene            complement(2449..2913)
                     /locus_tag="VFY44_01950"
     CDS             complement(2449..2913)
                     /locus_tag="VFY44_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5909224.1"
                     /translation="MPSWNEFEAAAPALAAEVRRRMDAYTHKTLATLRRDGAPRISGT
                     ETEIAEGDLWIGSMWDALKARDLQRDPRFALHSGSGDPPGWAGDAKLAGVAEEVTDPE
                     RVRAINGPKAPPGPSHLFRLDLREASTVGVQGDRLVIELWTAQGGTRTIRRA"
     gene            2987..>3210
                     /locus_tag="VFY44_01955"
     CDS             2987..>3210
                     /locus_tag="VFY44_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX5909225.1"
                     /translation="MADRLRELIDLVLGSLDEPGADGRALAERAHFSRDHLDRLLAAA
                     TGESPVALRRRLLLERAAWQLRSGAATASEA"
BASE COUNT          443 a         1189 c         1233 g          345 t
ORIGIN      
        1 ggggttcagg ctcagccacg cgaacacgcc gtccgggttc tctgagaagc gcaccagcgt
       61 gccgccatcg gccgggcggg gcttcatctc gagcgtgacg agggcggtgc cggcgggccg
      121 gcccttgacg cgcatccgga acagccgcgg gcgctcggcc tccacgatct cggagtggtc
      181 gtccagggtc agcgggccga acccgaccgt gtggtggaag cgcgagccgg ccgccggcca
      241 gtcggcgtcg acgtcgcgga tgtgcttcga cccgacgacc cagtacccgt agccaccggg
      301 gtcggcgagc gcgtcccaca ccgcctcggg cgccaccggc atgaaccgct cgttggtggc
      361 cacgtcagcc cgccaggtcg tcgtgggcgg cgagcgcggc cccgagggct ccgaagccgc
      421 ccgccagggc gaccgctccg ggcttggccc tgctgggcag cgccgacccg ggcgccagga
      481 gcgtggcgga caggtccgcg gcgtccgaca ccgcgcacgc ggccagccag gggcgcgcgt
      541 cgcggcccga cagggcggag gcggcgacgc cgacggacag caccaggtcg cgggcgccga
      601 gcccgcgcag ggccacggcc ccaccgggcg actcggcggc ggagccgacc cacgcccggg
      661 ccacccggtt cggggcggcc aggagcccga ccgcgtatcc gatccgcagt accgcgaagc
      721 cgaccgccac ttttcgtcca gagttgctca tcagtcggtc tccctacccg gcgaccacca
      781 tgggaaccag cagacggtgc cagaatcgct cccgtggcgg ccaagaagcc caagaccggc
      841 gccgacgcgc tcgacgaggt ctacctgggc gacttcgagg gcttcgtcgc ccgccgcgac
      901 gcgctggcca agcagctgcg ggccgacggc gacggcgacg aggccgaccg cgtcaaggca
      961 ctgaagaagc ccagccgcac ggcctgggcc gtgaaccagt tcgccgccaa cgggaagaag
     1021 ctgcggaacg acctgctcaa ggcgggcggc cggctgcggg aggcgtcgga gggcctggtg
     1081 tccggcgacg ccgaccgcga ggccatgctc aaggcgcgcg acggcgagcg cgccgcggtg
     1141 ggcgccgcgc tgaaggcgat cgccgcgctg gccgcggagt cggggccgga gctgaacgcg
     1201 gccgccaccg agcgcgtgcg ccagacactg cacgccgtgg ccctggacga cgaggtccgc
     1261 gagcagttcg aggccgcgcg gctgaccacc gaccacgagg ccagcgggct gggcgccggt
     1321 gggccggcga ccgcggccgg cgggggcgcc gcgaaggcga agccggccac ggccgcgaag
     1381 aagaagggcc gcgaccgcga ggccgagcgc aagcgccgcc ggcgcgacga gctgaaggcc
     1441 gccgaagccg aggcggcgaa gcgcgaccgc gatctgaagg acgccgacct ggagctggcc
     1501 gaggcgaaga aggcggccaa gcgcgcccag ggcgacctca agcgcgcgac cacgcggcag
     1561 gagaaggcga aggccgccgc cgacgacgcg gcggccaagg tccaggagct gggctaggcc
     1621 gcgggggcct cggcctcgat ctcgaaccac atccgccgcg ggtgcgggcc caggccccag
     1681 cgggtggtca gctcgtccag gatcggcgcc gtcgcctcct cggtcagtga ggccgatccg
     1741 gtgaccgggt cgccgtcctc gactacctcg ccgcgcaggt ggtcggggga gaggatctcg
     1801 acccgcaccg ccagtggcgc gccgttgccc gtgcggatga tgctggtgac catctcggtg
     1861 accaccaggc gcaggtccgc cagcagccgg ccgtccaggt cgtggaccag gtcgtccagc
     1921 gccatccgcg cagcccgggg cgcgtggagg tcaccgcgaa acatcaggtc ggacttcatg
     1981 gagggccagc tcccggcggg ttcttgcgca gcaggcgctg caccgggacg aatctaaccc
     2041 gcccggaccc ccggcgccag ggacccgcgc gtcacccggg agggtcgagt accacttgta
     2101 gggtgccgcc gggggcgagc cagcgcgagg ccagggcgcg ggcccggtcg ggctcgcggc
     2161 cgaacaggcc gacccggacg gcgaagaccc ggtcgaaccg gcggctgccc aggtcgacct
     2221 cctcgagcgc gcccgtcagg aactcggccg tccccgcccg gacgtgggcc cggttccggc
     2281 gctcggccgc ggcgatcatc ttcggggacc ggtcgatggc ggtgtaggtg ccgccgtcga
     2341 gctgctcgca cacgatcgtc gccgccacgc cgtggccgca gccgatctcc agcacccggt
     2401 ggccgggatc gagctccagc ccggctacga ccgcctcgag ccggccgctc acgcgcgccg
     2461 gatggtccgg gtcccgccct gcgccgtcca cagctcgatg accagccggt cgccctggac
     2521 gccgaccgtg gacgcctcgc gcaggtccag gcggaacagg tgcgaggggc cgggcggcgc
     2581 cttgggcccg ttgatcgccc gtacccgctc cgggtccgtg acctcctcgg ccaccccggc
     2641 cagcttggcg tcgcccgccc agcccggcgg gtcgccggag ccgctgtgca gcgcgaagcg
     2701 agggtcgcgc tgcaggtcgc gcgccttcag cgcgtcccac atggagccga tccacaggtc
     2761 gccctcggcg atctcggtct cggtgcccga gatccgcggc gcgccgtcgc ggcgcagggt
     2821 ggccagggtc ttgtgcgtgt aggcgtccat gcgccggcgc acctcggcgg ccagggcggg
     2881 cgcggcggcc tcgaactcgt tccagctggg catggcgcca cggtgaccgg tggcaggggg
     2941 gccgtccagc cacaggtcga atccgatcaa gcgcgaccat gtgcgcatgg ccgaccggct
     3001 gcgtgagttg atcgacttgg tgctgggctc gctggacgag cccggcgccg acggacgagc
     3061 cctggccgag cgggcgcact tctcgcgcga tcacctggac cgcctgctgg ccgcggccac
     3121 gggcgagtcc ccggtggcgc tgcgccggcg gctgttgctg gagcgggcgg cgtggcagct
     3181 gcgctccggc gcggccaccg cgtccgaggc
//
LOCUS       DASUPW010000084         6472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_848937, whole genome shotgun sequence.
ACCESSION   DASUPW010000084 DASUPW010000000
VERSION     DASUPW010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6472
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848937"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VFY44_01960"
     CDS             complement(<1..368)
                     /locus_tag="VFY44_01960"
                     /inference="COORDINATES: protein motif:HMM:NF025591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GXWXG domain-containing protein"
                     /protein_id="HEX5909226.1"
                     /translation="MSAQRLRALVETPGTVEDALAFFDSLDPVDPEAMIGEWDGGLVP
                     TGHPGEGMLDKLRWQGKAFRGADEVDPMVCLDEGGARAASDVMGSATLRRVEYRGVST
                     ATMVYDQHPVFDHFRRVDGET"
     gene            483..1394
                     /locus_tag="VFY44_01965"
     CDS             483..1394
                     /locus_tag="VFY44_01965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX5909227.1"
                     /translation="MASILPSPDEYAELAPTPGSPVWRAFNDVRLLNTSGFATLLQVA
                     HPTVGHGVHQYSSFVKDPWGRLLRTLDYVHGTIYGGPELAGEIGARVRNMHKSIKGTK
                     ADGSRYSAMEPDAFAWVHATLAHSVFEGYRVFATPMTSHEREAFWREWLGVGRLIGVR
                     ERDLPQRASGFKEYFDAVVDEQLQWTPAIPEVLDTLSAAPPPSVPGLRPGVWRLLRRP
                     MAAQLRVATVGLLPERLRRRLELPYSARERRTLAAMAALSRASGPAMRGPLAEFGPNY
                     VRWRHEALDRGDVAATGSAPAAKAAAA"
     gene            complement(1391..1804)
                     /locus_tag="VFY44_01970"
     CDS             complement(1391..1804)
                     /locus_tag="VFY44_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909228.1"
                     /translation="MNALPTWPPGTAAVLCVAGPHAIPVSTAIRAGDHRVLLALGGRR
                     ETLRRLREDPATALCVMAEGLAFTAKGPARVVQEGVEAAPALVVVELAVTEVVDHLAD
                     GRTAMDAAAGWHWLDDKAAESDPLIRAELQALAEG"
     gene            complement(1801..2364)
                     /locus_tag="VFY44_01975"
     CDS             complement(1801..2364)
                     /locus_tag="VFY44_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909229.1"
                     /translation="MGAAAPAPGRAATVVGEPPVNYKPDTPGGDRPDPRGIIFTAQPG
                     EANDLRLDRSGDDFVFRDRGAVLTASGFGCRQAGRHSVRCRATNFGPEDAEQVRVRTH
                     AGNDRVVVASGVGTIAYTSVFTGTGQDRVRLSAFGEVYSGRGNDRVELRGTGEMEADC
                     GSGRDVYIGERGLSRRCERKIFRRSLR"
     gene            complement(2441..4633)
                     /locus_tag="VFY44_01980"
     CDS             complement(2441..4633)
                     /locus_tag="VFY44_01980"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5909230.1"
                     /translation="MDRLTAVIERRRRIVIAVWLVLLVAAAPLAMRQTEHLTSGGFAV
                     PGSDSEAVDRGLAEFDRAQAHSLAVIVARRPGASDAEVRAAVDRVDRVAAELPHAELS
                     SQAAARAKRQAARAPVVTLPLTVQADQDQASDLAVDLRDELKVGEGARNGVELYAVGQ
                     EALWAGMQDLAKEDLEKAEVTGFPIVLLILLAVFGSLAAAALPLALGFASVTITGAAI
                     YFLAQLTEMSVFVTNVSSMIGIGVAVDYSLFLLARYREEVRAGVDPEQARRTAMHTSG
                     IAVLFSGVTVIVALAGLFLVDSTTIRSMAMGAIVVVAVSILAALTLLPALMRVLGKRA
                     YTRGRTATVLGVVARAVRWRRRRPGSTDPDNQRAGFWQRWTDTVMRRPLLFAGASAAL
                     MLALAVPALSLETGDGALRQFPADNETRQGAELAARQAGPGASNPAQVLVETGDRRAL
                     ARYAAQARRDPEAAAVGRPELSRDGRSALITVEPRHDPESPEARAMVARLDAIPAAGA
                     DVRVGGATAAVEDFKNLVSGSMWKIALFVLAFSYLVLLVLLRSVLLPLKAVLMNLLSV
                     GAAYGVLVIVFQWGWLDGFAGFESLGYVNTMTPPLLLAIVFGLSMDYEVFLLSRIRER
                     YDATGDTKRSVAQGLAASAATISSAALIMVAVFAVFAGTGVPSVKEIGLGLAVAIALD
                     ATIVRLVLVPATMELMGKWNWWLPRSLDRVLPHTDFEAPAPEPHPQGT"
     gene            complement(4690..5301)
                     /locus_tag="VFY44_01985"
     CDS             complement(4690..5301)
                     /locus_tag="VFY44_01985"
                     /inference="COORDINATES: protein motif:HMM:NF037009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5909231.1"
                     /translation="MEAASTAPRKLDGEKAGRIVAAMRESVGARGAAGSTFDVVAREA
                     GVSRGLLHYYFGSKERLMVEVVRRDADAFVTELETSLEQATTVDEIIASLVRTLDAFV
                     QGEPGTQAVIYEMLSASRHSEEIRAEMAELYRTWRHHLAGALRHKEQEGVITLAGDPE
                     SVASLIFAIGDGLGLQLISDPEWARETSLDVAMDTARHLLGAT"
     gene            complement(5387..5854)
                     /locus_tag="VFY44_01990"
     CDS             complement(5387..5854)
                     /locus_tag="VFY44_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909232.1"
                     /translation="MASKKKKAAKAGSGAIAAGQAARNNEYVQRLIDDDSLRDDLRTA
                     FESARKAYDRINGKGPQKALDDKKTQKELKEAATSLKSAADSLRGGRKKKRGKGKFLL
                     IGIVGAGLALGLSEGLRNKVLDALFGAEEEFEYTSSTTSNSGSASSSTEKVGA"
     gene            complement(5911..>6472)
                     /locus_tag="VFY44_01995"
     CDS             complement(5911..>6472)
                     /locus_tag="VFY44_01995"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEX5909233.1"
                     /translation="TGLAWTPVGGDVLFVEATSYPGEGKLQVTGQLGDVMKESAAAAL
                     SYIRHDVRALGDDVPDDWFRENDVHVHVPAGAIPKDGPSAGITMATALASLVTGRPVR
                     ADTAMTGEITLTGLVLPIGGLKEKALAAQRAEVKRVIAPRRNEADLDEFPPNLLKHMK
                     FVFVDTVDEVLEAALDPVKRRLAKVG"
BASE COUNT          884 a         2408 c         2296 g          884 t
ORIGIN      
        1 gtctcgccgt ccacgcgccg gaagtggtcg aacaccgggt gctggtcgta gaccatcgtc
       61 gcggtgctga cgccgcggta ctccacgcgc cgcagcgtcg ccgagcccat cacgtcgctg
      121 gccgcgcgcg cgccgccctc gtccaggcag accatcgggt ccacctcgtc ggcgccgcgg
      181 aaggccttgc cctgccagcg cagcttgtcc agcatcccct cgcccggatg gccggtgggc
      241 accaggccgc cgtcccactc cccgatcatc gcctcggggt cgaccggatc gagcgagtcg
      301 aagaaggcca gggcgtcctc gaccgtcccc ggcgtctcga ccagcgctcg gagccgctgc
      361 gcgctcaccc ggcggacgct aacgcagccc cggacggccg ggggtggccg ggccacccac
      421 ccgcccggtc gagtggggat gcgccacacc cacgcggcgc cgggtcgcgt aagggtccgg
      481 gcatggcctc gatcctgcct tcccccgacg agtacgcgga gctggccccc acacccggat
      541 cgccggtctg gcgcgcgttc aacgacgtcc ggctgctgaa cacgtccggg ttcgcgacgc
      601 tgctgcaggt cgcccacccc accgtgggcc acggcgtcca ccagtactcc tcgttcgtca
      661 aggatccctg ggggcgcctg ctgcgcacgc tcgactacgt gcacgggacc atctacggcg
      721 gccccgagct ggccggcgag atcggggccc gcgtgcgcaa catgcacaag tcgatcaagg
      781 gcacgaaggc cgacggctcg cgctactcgg cgatggagcc ggacgcgttc gcctgggtgc
      841 acgccacgct ggcccactcg gtgttcgagg gctaccgggt gttcgcgacg ccgatgactt
      901 cccacgagcg ggaggcgttc tggcgcgagt ggctgggggt cggccgcctg atcggcgtcc
      961 gcgagcgcga tctgccgcag cgcgcctcgg gcttcaagga gtacttcgac gcggtggtcg
     1021 acgagcagct gcagtggacc ccggcgatcc ccgaggtgct ggacaccctc agcgcggctc
     1081 cgcctcccag cgtgcccggg ctgcggccgg gtgtgtggcg gctgctccgc aggccgatgg
     1141 ccgcccagct tcgcgtggcc accgtcggcc tgctgcccga gcggctgcgg cgcaggctcg
     1201 agctgcccta ctccgcccgc gagcggcgga cgctcgcggc gatggccgcc ctgtcgcgcg
     1261 cgtcgggccc ggcgatgcgc ggcccgctgg ccgagttcgg ccccaactac gtgcgctggc
     1321 gccacgaggc gctggaccgg ggcgacgtgg ccgcgaccgg ctcggcgccg gccgccaagg
     1381 ccgccgcggc ctagccctcg gccagcgcct gcagctcggc ccggatcagc gggtccgact
     1441 cggcggcctt gtcgtcgagc cagtgccacc cggccgcggc gtccatggcc gtccgcccgt
     1501 cggccaggtg gtcgaccacc tcggtcaccg caagctccac gaccaccagc gccggcgccg
     1561 cctcgacgcc ctcctgcacc acgcgcgccg ggcccttggc ggtgaaggcc aggccctcgg
     1621 ccatcacgca cagcgcggtg gccggatcct cgcgcagccg gcgcagcgtc tcccgcctgc
     1681 cgccaagtgc cagcagcacc cggtggtcgc cggcgcggat ggcggtcgag acggggatcg
     1741 cgtgcgggcc ggcgacgcac agcaccgcgg cggtgccggg cggccaggtg ggcagggcgt
     1801 tcaccgcaat gaccgccgga agatcttccg ctcgcagcgc ctcgagagcc cgcgctctcc
     1861 gatgtacacg tcgcggccgc ttccgcagtc ggcctccatc tcgccggtcc cacgcagctc
     1921 gacgcggtcg ttgccccggc cggagtagac ctcgccgaac gcggacaggc gtacgcggtc
     1981 ctggcccgtg cccgtgaaca ccgacgtgta ggcgatcgtg ccgaccccgc tggcgaccac
     2041 caccctgtcg ttacccgcgt gggttctgac gcgcacctgc tcggcatcct cggggccgaa
     2101 gttggtggcc cggcaacgca cggaatgcct ccccgcctgc cgacaaccga aaccggacgc
     2161 cgtgagcacg gctccccgat ctcggaagac gaagtcgtca ccggaacggt cgagtcgcag
     2221 gtcgttggcc tccccgggct gggcggtgaa gatgatcccc cgcgggtcgg gccggtcacc
     2281 gccgggcgtg tccggcttgt agttgaccgg cggctccccg acgacggtgg cggcccggcc
     2341 gggggccggt gctgcggcac ccatcgccgc aatcatcagg atcagagtcc cgcgcagacg
     2401 caccccgaca gtgaaacacg cgcacggtgg cgcggttgcg tcaggtcccc tgggggtgtg
     2461 gctccggcgc cggcgcctcg aagtcggtgt gcgggagcac ccggtccagc gagcgcggca
     2521 gccaccagtt ccacttgccc atcagctcca tcgtggccgg caccagcacc aggcgcacga
     2581 tcgtcgcgtc cagggcgatg gccaccgcca ggcccaggcc gatctccttc accgacggca
     2641 cgcccgttcc ggcgaacacc gcgaacaccg cgaccatgat cagcgccgcg ctggagatcg
     2701 tggccgcgct ggcggccagc ccctgggcca ccgagcgctt ggtgtcgccg gtggcgtcgt
     2761 agcgctcgcg gatgcggctg agcaggaaga cctcgtagtc catcgacagg ccgaagacga
     2821 tggccaggag cagcggcggc gtcatggtgt tcacgtagcc cagcgactcg aagccggcga
     2881 agccgtccag ccagccccac tggaacacga tcaccagcac cccataggcg gcgcccaccg
     2941 agagcaggtt catcagcacc gccttgagcg gcagcagcac cgagcgcagc agcaccagca
     3001 ggaccaggta gctgaaggcc agcacgaaca gcgcgatctt ccacatcgat ccgctgacca
     3061 ggttcttgaa gtcctcgacg gcggccgtgg cgccgcccac gcggacgtcg gcgccggccg
     3121 cggggatcgc gtccaggcgg gcgaccatgg cccgggcctc ggggctctcg gggtcgtggc
     3181 ggggctccac ggtgatcagg gcggagcgcc cgtcgcgcga gagctcgggg cggccgaccg
     3241 cggccgcctc cggatcgcgc cgggcctggg cggcgtacct ggcgagcgcg cgacggtcgc
     3301 cggtctccac cagcacctgc gccgggttgg acgcgcccgg cccggcctgg cgcgcggcca
     3361 gctcggcgcc ctggcgcgtc tcgttgtcgg cggggaactg gcgcagcgcg ccgtcgccgg
     3421 tctccagcga cagggcgggc acggccagcg ccagcatcag cgccgcgctg gcaccggcga
     3481 acagcagcgg ccggcgcatg acggtgtcgg tccagcgctg ccagaagccg gcccgctggt
     3541 tgtccggatc ggtcgagccc gggcggcgcc ggcgccagcg caccgcgcgc gccaccacgc
     3601 cgaggaccgt ggccgtgcgc ccgcgcgtgt aggcgcgctt gcccagcacc cgcatcaacg
     3661 ccggcagcag cgtcagggcg gccaggatcg acaccgccac gaccacgatc gcccccatgg
     3721 ccatcgagcg gatcgtggtg gagtccacca ggaacaggcc ggccagcgcc acgatcacgg
     3781 tcacgccgga gaacagcacg gcgatgcccg acgtgtgcat cgcggtccgg cgcgcctgct
     3841 cggggtcgac ccccgcgcgc acctcctcgc ggtagcgggc cagcaggaac agcgagtagt
     3901 ccaccgcgac gccgatgccg atcatcgacg agacgttggt cacgaacacc gacatctcgg
     3961 tcagctgggc caggaagtag atggcggcgc cggtgatggt caccgaggcg aagcccagcg
     4021 ccagcggcag ggcggcggcg gccagcgagc cgaacacggc cagcaggatc aggaggacga
     4081 tcgggaagcc cgtgacctcg gccttctcca ggtcctcctt ggccaggtcc tgcatgccgg
     4141 cccacagggc ctcctggccg acggcgtaca gctcgacgcc gttgcgcgcg ccctcgccca
     4201 ccttcagctc gtcgcgcagg tccacggcca ggtcgctggc ctggtcctgg tcggcctgga
     4261 cggtcagcgg cagggtgacc acgggcgccc gcgctgcctg gcgcttggcg cgggcggctg
     4321 cctgggagga cagctcggcg tgcggcagct cggccgccac gcggtcgacg cggtccaccg
     4381 cggcgcgcac ctcggcgtcg gaggcgcccg ggcgacgggc cacgatcacc gccaggctgt
     4441 gggcctgcgc gcggtcgaac tcggccaggc cgcggtccac ggcctcggag tccgagccgg
     4501 gcacggcgaa gccaccgctg gtcaggtgct cggtctggcg catggccagc ggcgcggcgg
     4561 ccaccaggag caccagccag acggcgatca cgatccggcg gcggcgttcg atcaccgcgg
     4621 tgagccggtc catcgcagcc tgcatcggcc tatcagaccg ccgctacgaa gcggggtgtg
     4681 tgaggtgcgt cacgtggcgc ccagcagatg gcgggccgtg tccatggcca cgtccagcga
     4741 ggtctcgcgg gcccactcgg gatcggagat cagctgcagg cccaggccgt cgccgatggc
     4801 gaagatcagc gacgccaccg actcggggtc gccggccagc gtgatcacgc cctcctgctc
     4861 cttgtggcgc agcgcgccgg ccaggtggtg gcgccaggtg cggtagagct cggccatctc
     4921 ggcgcggatc tcctcgctgt ggcgcgaggc gctgagcatc tcgtagatca cggcctgggt
     4981 gccgggctcg ccctggacga aggcgtccag cgtgcggacc agcgacgcga tgatctcgtc
     5041 caccgtggtg gcctgctcca ggctggtctc cagctcggtc acgaacgcgt ccgcgtcacg
     5101 ccggaccacc tcgaccatca ggcgttcctt ggagccgaag tagtagtgca gcagcccgcg
     5161 cgagacgccg gcctcgcggg ccacgacgtc gaaggtcgag cccgccgcgc cgcgggcacc
     5221 cacgctctcg cgcatcgcgg cgacgatccg gccggccttc tcgccgtcca gcttgcgtgg
     5281 tgctgtcgag gctgcctcca cgcggcggag tatgccgcac cgaccccgga aacgagagag
     5341 gcgccccgca gggcgcctct cgcaacttcc gggatggggc gagagcctag gccccgacct
     5401 tctccgtgct gctggaggcg gatccggagt tcgacgtcgt ggacgacgtg tactcgaact
     5461 cctcctcggc gccgaacagg gcgtcgagca ccttgttgcg caggccctcg ctgaggccga
     5521 gcgccaggcc ggcaccgacg atgccgatga gcaggaactt gcccttgccg cgcttcttct
     5581 tgcggccgcc acgaagggag tcggccgcgc tcttgagcga ggtcgcggct tccttcagct
     5641 ccttctgcgt cttcttgtcg tcgagcgcct tctgcgggcc cttgccgttg atgcggtcgt
     5701 aggccttgcg cgccgactcg aaggccgtgc gaaggtcatc gcgcagcgag tcgtcgtcga
     5761 tcagtcgctg cacgtactcg ttgttgcggg cggcctgacc agcagctatc gctcctgatc
     5821 ccgcctttgc ggccttcttc ttcttcgatg ccatggctct cctcgagtgg gcttgctggt
     5881 ggaacagact acccagagtt ggcgcctcaa tcagccgacc tttgccagtc gtcgcttgac
     5941 cgggtccagc gccgcctcca gtacctcgtc gacggtgtcc acgaagacga acttcatgtg
     6001 cttgagaagg ttgggcggga actcgtccag gtcggcctcg ttgcgccgcg gcgcgatcac
     6061 ccgcttgacc tcggcgcgct gggccgccag cgccttctcc ttgagcccgc cgatgggcag
     6121 cacgaggccg gtcagcgtga tctcgccggt catcgcggtg tcggcccgga cgggccggcc
     6181 ggtgaccagc gacgccagcg cggtggccat cgtgatgccc gcgctggggc cgtccttcgg
     6241 gatcgcgccg gcgggcacgt gcacgtgcac gtcgttctcg cggaaccagt cgtcgggcac
     6301 gtcgtcgccc agagcgcgca cgtcgtggcg gatgtacgac agggccgcgg cggccgactc
     6361 cttcatcacg tcgcccagct ggccggtgac ctggagcttg ccctcaccgg gataggaggt
     6421 ggcctccacg aacaggacgt cgccgcccac aggggtccag gccaggccgg tg
//
LOCUS       DASUPW010000085         3296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1541334, whole genome shotgun sequence.
ACCESSION   DASUPW010000085 DASUPW010000000
VERSION     DASUPW010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1541334"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="VFY44_02000"
     CDS             complement(<1..945)
                     /locus_tag="VFY44_02000"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5909234.1"
                     /translation="MLTTATTTPLVTASDPDWDATRQVFNLALDLQPAAIALPRDVGE
                     VIAAVEYARERGLRVAPQATGHNAAAYGPLEDALLVDVRRLQEVSIDPCGQRVRVGAG
                     VKWERVVPKLSEHGLAALHGSSPDVGIAGYSLGGGMGWLARKHGLQANSVTAIELVTA
                     DGTFRRVDATHEPDLFWALRGGNGNFGVVTAIEFRVFPVEELYAGAMFFPFERTADVL
                     SAFTELAPAAPDELMAWTTLLQFPDVPFVPEPFRGGSFAVLMGAYLGEEAEGRELLRG
                     VRDLGPAMDTFAMVPPVELSELAMDPPEPLPVMMTTAQL"
     gene            1066..2136
                     /locus_tag="VFY44_02005"
     CDS             1066..2136
                     /locus_tag="VFY44_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5909235.1"
                     /translation="MALLAPESTLEGLEELAHADLTAQELIEEAAERIARVVPTDGHF
                     FSATDPETTLAMGAGAIKNLPFEMCQTHWDYEFLVPDYLKFADIAQGPVPVVDLHDAT
                     GGRPERSPRWREFGGYTGLRSEVRMTFAVDGATWGIGQLNRTADAPLFSDEEKDWLAR
                     VAPAIGAGLRKALLAPAETTAADRGPGLVLLDPDGSVASVNREAAQWLDELRVGPVFP
                     GQDHAVPFEAFSYAARAQDAPERARVRTREGVWLTMHASLLEGTGQLALIIEPAKAGE
                     VAPLIIEAYELTERELDVTRRIARGLGTSEIAAELYLSPHTVRDHVKAIFEKVGVRSR
                     GELVATVFADHYAQPGHYSAPA"
     gene            complement(2146..>3296)
                     /locus_tag="VFY44_02010"
     CDS             complement(2146..>3296)
                     /locus_tag="VFY44_02010"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX5909236.1"
                     /translation="IQEGVVSLGGYGDVFVRNVQCSGVIPQISLIMGPCAGGAVYSPA
                     MTDFIFMVKETSHMFITGPDVIKTVTGEEVEFEELGGAMTHNQKSGVAHFAAEDEESC
                     LEDVRYFMSFLPQNNLETPPQMDSGDPHDRMDTELDSVVPDSPNKPYDMRTVVGHIVD
                     DGDFFEVQEHFALNIIVGFARLGGKPVGIVGNQPSQMAGVLDIDASEKAARFVRFCDA
                     FNIPIVTFTDVPGFLPGTDQEWGGIIRHGAKLLYAFTEATVPKLTVVTRKAYGGAYDV
                     MNSKHMLADFNFAWPQAEVAVMGPEGAVNIIYRRDIAASPTPDERRQKLMDDYKARFA
                     NPYSAAERGYIDDVIEPHETRPKLIHALKMLETKRVAGPKRKHGNIPL"
BASE COUNT          463 a         1154 c         1201 g          478 t
ORIGIN      
        1 gagctgggcc gtggtcatca tcaccggcag cggctccggc gggtccatgg ccagctcgct
       61 gagctcgacg ggcggcacca tggcgaacgt gtccatggcc ggccccaggt cgcggacccc
      121 gcgcagcagc tcgcgcccct cggcctcctc gcccaggtag gcgcccatca gcacggcgaa
      181 cgagccgccc cggaagggct ccggcacgaa cggcacgtcc gggaactgca gcagcgtggt
      241 ccacgccatc agctcgtccg gcgcggccgg ggccagctcg gtgaacgcgc tcagcacgtc
      301 ggccgtgcgc tcgaacggga agaacatggc acccgcgtag agctcctcga cggggaagac
      361 gcggaactcg atcgccgtga ccacgccgaa gttgccgttg cccccgcgca gggcccagaa
      421 caggtcgggc tcgtgggtgg cgtcgacccg gcggaaggtg ccgtcggcgg tgaccagctc
      481 gatggccgtg acgctgttgg cctggagccc gtgcttgcgg gccagccagc ccatgccgcc
      541 ccccagcgag tagccggcga tgcccacgtc gggggaggac ccgtgcagcg cggccaggcc
      601 gtgctcggac agcttgggca ccacgcgctc ccacttcacg cccgcgccca cgcggacgcg
      661 ctggccgcac gggtcgatcg agacctcctg gagccggcgg acgtccacca gcagcgcgtc
      721 ctccagcggg ccgtaggcgg ccgcgttgtg gccggtggcc tggggcgcca cccgcagccc
      781 gcgctcgcgg gcgtactcca cggcggcgat cacctcgccg acgtcgcgcg ggagcgcgat
      841 cgcggcgggc tgcaggtcca gcgccaggtt gaacacctgg cgcgtggcgt cccagtcggg
      901 gtcggaggcg gtgacgagcg gggttgtggt ggcggtggtg agcatgcgtc gatgctgcgc
      961 cgccgcgcgc cctgcggcca tcccgcgctt gagggtgatc gccgccgccc atacccctca
     1021 tttgcggggg gtgacatgtc cccggagcgg gtgtacgttg ggcgcatggc cctactcgcg
     1081 cccgagagca ccctcgaggg gctcgaggag ctcgcgcacg ccgacctcac ggcccaggag
     1141 ctgatcgagg aggcggccga gcggatcgcg cgggtcgtcc ccacggacgg ccacttcttc
     1201 tccgccaccg accccgagac cacgctcgcg atgggggccg gcgccatcaa gaacctcccg
     1261 ttcgagatgt gccagacgca ctgggactac gagttcctgg tgccggacta cctcaagttc
     1321 gccgacatcg cccagggccc ggtcccggtg gtggacctgc acgacgccac cggtgggcgg
     1381 ccggagcgct caccccgctg gcgcgagttc ggcggctaca ccgggctccg gtccgaggtg
     1441 cgcatgacct tcgccgtcga cggggccacg tgggggatcg gccagctcaa ccgcacggcg
     1501 gacgcgccgc tcttctccga cgaggagaag gactggctcg cgcgtgtggc gcccgccatc
     1561 ggtgcggggc tgcgcaaggc gctcctggcg ccggccgaga ccacggcggc ggaccgcggg
     1621 cccggcctgg tgctgctgga tcccgacggc tccgtcgcgt cggtcaaccg cgaggcggcc
     1681 cagtggctgg acgagctgcg cgtggggccg gtcttccccg gccaggacca cgccgtgccc
     1741 ttcgaggcct tcagctatgc cgcccgggcg caggacgcgc ccgaacgcgc ccgggtccgc
     1801 acccgcgagg gcgtctggct caccatgcac gcctcgctgc tcgagggcac cggccagctg
     1861 gccctgatca tcgagccggc caaggccggc gaggtggcgc cgctgatcat cgaggcctac
     1921 gagctcaccg agcgcgagct cgacgtcacg cgccggatcg cccgcgggct gggcacctcg
     1981 gagatcgccg ccgagctgta cctctcaccg cacacggtgc gcgaccacgt caaggcgatc
     2041 ttcgagaagg tcggcgtcag gagccgcggc gagctggtgg ccacggtgtt cgccgaccac
     2101 tacgcgcagc cggggcacta cagcgcgccg gcctagggga aggccctaca gagggatgtt
     2161 gccgtgcttg cgcttcggcc cggcgacgcg cttggtctcc agcatcttca gcgcgtggat
     2221 cagcttcggg cgcgtctcgt gcggctcgat cacgtcgtcg atgtagccgc gctcggccgc
     2281 cgagtacggg ttggcgaagc gcgccttgta gtcgtccatc agcttctggc ggcgctcgtc
     2341 cggcgtcggt gaggcggcga tgtcgcggcg gtagatgatg ttcaccgcgc cctcgggccc
     2401 catgaccgcg acctcggcct gcggccaggc gaagttgaag tcggccagca tgtgcttgga
     2461 gttcatgacg tcgtaggcgc cgccgtaggc cttgcgggtg accacggtca gcttgggcac
     2521 ggtggcctcg gtgaacgcgt acagcagctt ggcgccgtgc cggatgatgc cgccccactc
     2581 ctggtcggtg ccgggcagga agccgggcac gtcggtgaag gtgacgatcg ggatgttgaa
     2641 ggcgtcgcag aagcgcacga agcgcgcggc cttctcggac gcgtcgatgt ccaggacgcc
     2701 ggccatctgc gacggctggt tgcccacgat gcccaccggc ttgccgccca ggcgggcgaa
     2761 gccgacgatg atgttcagcg cgaagtgctc ctgcacctcg aagaagtcgc cgtcgtcgac
     2821 gatgtggccg accacggtgc gcatgtcgta gggcttgttg ggggagtcgg gcacgaccga
     2881 gtccagctcg gtgtccatgc ggtcgtgcgg gtcgccgctg tccatctgcg gcggggtctc
     2941 caggttgttc tggggcagga aggacatgaa gtagcgcacg tcctccaggc acgactcctc
     3001 gtcctcggcg gcgaagtggg ccacgcccga cttctggttg tgggtcatgg cgccgcccag
     3061 ctcctcgaac tccacctcct cgccggtgac cgtcttgatc acgtcggggc ccgtgatgaa
     3121 catgtgcgag gtctccttga ccatgaagat gaagtcggtc atggcggggg agtacacggc
     3181 gccgccggcg cacgggccca tgatcagcga gatctgcggg atcacgccgg agcactgcac
     3241 gttgcggacg aagacgtcgc cgtagccgcc gagggagacg acgccctcct ggatcc
//
LOCUS       DASUPW010000086         3981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_849954, whole genome shotgun sequence.
ACCESSION   DASUPW010000086 DASUPW010000000
VERSION     DASUPW010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849954"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VFY44_02015"
     CDS             <1..445
                     /locus_tag="VFY44_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5909237.1"
                     /translation="KGGFPGGVTYCGTKHFVVGASEGILAEVKDFGIDVSCVMPVVVK
                     TELAAGLQETRGVKHVTPEEVADEIVAAMEQPSFDVFVPRSVGHITRAMSVLPRGGRE
                     AIGRALKADKVLAQVDDKARQAYELRASRSEPSLNAGEDQKQLTK"
     gene            450..1220
                     /locus_tag="VFY44_02020"
     CDS             450..1220
                     /locus_tag="VFY44_02020"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5909238.1"
                     /translation="MSVAKAAVVGAGTMGRDIAKVLAGAGVEVLLKDVDPALVEIGEE
                     GVTGTTSYDGFGDVEFVIEAVPEVMELKHAVFSELDAVTPGHAILASNTSCLSITEIG
                     EATTRPDRVVGVHFFYPATMARLIEVVEGADTSDETVAAATRFAQQLRKTPIRCGEAP
                     GFVVNRILTAASSEAWRMVDEEGVSTEEVDAAIKESGGPVGPFHLVDLLGLDTVLHVA
                     EHLHASYGGERFHVSEAMRALVRQGDLGVKTGKGFYEH"
     gene            1210..2283
                     /locus_tag="VFY44_02025"
     CDS             1210..2283
                     /locus_tag="VFY44_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5909239.1"
                     /translation="MSIEQLPERVALKTLVEACLVLEDGEASAKDIEVGMMMGAGIVP
                     GPFARADEQGLDTALEALERAQAEWGDAFAPPVVLRRLVAQGRLGRKSGQGFFAYPRP
                     DDARETVALETRGDVGILWLDRPPAHALSPELIGELGELWASVDGKLRAVVFASSNIF
                     TFSAGADIKAFTRMDPAQGRELIEAAHGLFRSFERSSTVTIAAVNSIAFGGGCELAMA
                     CDLRLAAESASFGQPEIDLGIIPGFGGTQRLPRLVGQAKALEMNLTADPIDAYEAWRV
                     GLANRVVPDHELLDTALLWARDLAGQAPLAVEQIKRVSGAGDLDQGIEAEKEAFAGIF
                     GSDDATEGIAAFLGKRQASFKGR"
     gene            2298..3023
                     /locus_tag="VFY44_02030"
     CDS             2298..3023
                     /locus_tag="VFY44_02030"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEX5909240.1"
                     /translation="MAELLRSARSAVVLTGAGISVPSGIPDFRTPGTGIWADVDPMEV
                     AHIDVWRRDPDRFWHFYGARFASLREVEPNPAHAAVAELERRGLIDGVITQNIDRLHR
                     AAGSSEVVEVHGSIEWSRCPVCGTRVELERVVEMLEAAGGAPECPACVTPLKPEVVLF
                     GEMLPMEAIARAEQLAAGADLMICIGSSLVVHPVAGLPEMTLAAGGQVAVITGSETPY
                     DDRATVRMGGDVVDELESVLAAL"
     gene            complement(3168..>3981)
                     /locus_tag="VFY44_02035"
     CDS             complement(3168..>3981)
                     /locus_tag="VFY44_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909241.1"
                     /translation="FGYLLGPADGRYLVRAPGAEQVERVLALRTLGAPQRRLMRGRRG
                     TEVEQAVAEPVPTSRATVILPEAFAGSGPAQEWLARLRDSEDEREAEIAAAVVVLNRA
                     LHAHRTARADGAARDVTQASALVVRLGFGSGEQVAEGRFDEGWELPRGVAKVKRSMEA
                     PEERFAALLGGRESTLACEELVLRARADADAGRDREAALQARIALESLLSELEKLPDS
                     RRQALEEDRGPVGQAANSALRGPVDRSAAEAVEAAVTRMEAALRAHRLGTGR"
BASE COUNT          526 a         1411 c         1526 g          518 t
ORIGIN      
        1 caagggcggt ttccccggcg gcgtgaccta ctgcggcacc aagcacttcg tggtcggcgc
       61 gtccgagggc atcctggccg aggtcaagga cttcggcatc gacgtctcgt gcgtgatgcc
      121 ggtggtggtc aagaccgagc tggcggccgg gctccaggag acgcgcggcg tcaagcacgt
      181 gacccccgag gaggtggccg acgagatcgt cgcggccatg gagcagccca gtttcgacgt
      241 gttcgtcccg cgctcggtgg gccacatcac gcgcgcaatg agcgtgctgc cgcgcggcgg
      301 ccgggaggcc atcgggcgcg cgctcaaggc cgacaaggtg ctggcccagg tcgacgacaa
      361 ggcgcgtcag gcctacgagc tgcgcgccag ccgctcggag ccgagcctga acgccggtga
      421 ggaccagaag cagctgacga agtgatcctg tgagcgtcgc caaggccgcc gtcgtcggtg
      481 ccggcaccat gggccgggac atcgccaagg tgctggcggg agccggcgtc gaggtgctgc
      541 tcaaggacgt ggaccccgcg ctggtcgaga tcggcgagga gggtgtgacc ggcaccacct
      601 cctacgacgg cttcggcgac gtggagttcg tgatcgaggc ggtcccggag gtcatggagc
      661 tcaagcacgc ggtgttctca gagctggacg ccgtcacccc cggccacgcg atcctggcgt
      721 cgaacacctc gtgcctgtcg atcaccgaga tcggcgaggc caccacccgc cccgaccgcg
      781 tggtcggcgt ccacttcttc tacccggcca ccatggcccg gctgatcgag gtggtggagg
      841 gggccgacac ctccgacgag accgtcgccg cggccacgcg cttcgcccag cagctgcgca
      901 agacgccgat ccgctgcggg gaggcgccgg gcttcgtggt caaccggatc ctgaccgccg
      961 ccagctcgga ggcctggcgg atggtcgacg aggagggcgt gtccaccgag gaggtggacg
     1021 cggcgatcaa ggagtccggc ggccccgtcg gcccgttcca cctggtcgac ctgctgggcc
     1081 tggacaccgt cctgcacgtg gccgagcacc tgcacgccag ctacggcggc gagcgcttcc
     1141 acgtgtccga ggccatgcgc gcgctggtgc gccagggcga cctcggcgtg aagaccggga
     1201 agggcttcta tgagcattga gcagctgccc gagcgggtgg cgctgaagac gctggtggag
     1261 gcctgcctgg tcctggagga cggcgaggcc tcggccaagg acatcgaggt ggggatgatg
     1321 atgggcgccg gcatcgtgcc cggcccgttc gcccgcgccg acgagcaggg cctggacacg
     1381 gcgctggagg cactggagcg cgcgcaggcc gagtggggcg atgcgttcgc gccgccggtg
     1441 gtgctgcgcc ggctggtggc ccaggggcgc ctgggcagga agagcggcca gggcttcttc
     1501 gcctacccgc ggcccgacga cgcccgcgag accgtcgcgc tggagacccg cggcgacgtg
     1561 ggcatcctgt ggctggaccg gcccccggcc cacgcgctgt cgcccgagct catcggcgag
     1621 ctcggcgagc tgtgggcgtc ggtggacggg aagctgcgcg cggtcgtgtt cgccagctcg
     1681 aacatcttca cgttctccgc cggggccgac atcaaggcct tcaccaggat ggacccggcc
     1741 cagggcaggg agctgatcga ggcggcccac ggcctcttcc gctcgttcga gcgctcgtcc
     1801 accgtcacga tcgccgcggt caactcgatc gccttcggcg gcggctgcga gctggccatg
     1861 gcctgcgacc tgcgcctggc cgccgagtcg gccagcttcg gccagcccga gatcgacctg
     1921 ggcatcatcc cgggcttcgg cgggacccag cggctgccgc ggctggtggg ccaggcgaag
     1981 gcgctggaga tgaacctgac cgcggacccg atcgacgcct acgaggcgtg gcgggtgggc
     2041 ctggccaacc gggtcgtgcc cgaccacgag ctgctggaca cggcgctgct gtgggcgcgc
     2101 gacctggccg gccaggcgcc gctggcggtg gagcagatca agcgcgtgtc cggcgccggc
     2161 gacctggatc aggggatcga ggccgagaag gaggccttcg ccggcatctt cggctccgac
     2221 gacgcgacgg aagggatcgc ggcattcctg ggcaagcgcc aggcgagctt caagggcagg
     2281 taggcccggc cgcggagctg gccgagctgc tgcgctcggc ccggtccgcg gtggtgctca
     2341 ccggcgcggg gatctcggtg ccgtcgggca tcccggactt ccgcaccccg ggcaccggca
     2401 tctgggccga cgtggacccg atggaggtcg cccacatcga cgtgtggcgg cgcgatcccg
     2461 accgcttctg gcacttctac ggcgcccgct tcgcgtcgct gcgcgaggtg gagcccaacc
     2521 cggcccacgc cgccgtggcc gagctggagc ggcgcggcct gatcgacggc gtgatcacgc
     2581 agaacatcga ccggctgcac cgcgccgcgg gcagcagcga ggtcgtcgag gtgcacggct
     2641 cgatcgagtg gagccggtgc ccggtctgcg gcacgcgcgt ggagctggag cgggtggtcg
     2701 agatgctgga ggcggcgggc ggagcgccgg agtgcccggc ctgcgtgacg ccgctgaagc
     2761 ccgaggtggt gctgttcggc gagatgctgc cgatggaggc catcgcccgc gccgagcagc
     2821 tggccgccgg cgccgacctg atgatctgca tcggctcgtc gctggtggtg cacccggtgg
     2881 caggcctgcc ggagatgacg ctggccgccg gcgggcaggt ggccgtgatc accgggagcg
     2941 agacgccgta cgacgaccgc gccaccgtcc ggatgggcgg cgacgtggtc gacgagctgg
     3001 aatccgtgct ggccgctctc tgagggagcc gtaggccggg ccgccgcccc ggggagagaa
     3061 gggacgacgg ccgcgcggcc tatgactcac cccatcctgc gcgccccgcg ccccgctggt
     3121 tatcgaccgg tggccaaaga tcgccgggat cggctcgacg gccggtccta ccggccggtg
     3181 cccagccggt gcgcgcgcag ggccgcctcc atgcgggtca cggcggcctc gacggcctcg
     3241 gccgcggagc ggtccacggg cccgcgcagc gcggagttcg ccgcctggcc gaccgggcca
     3301 cggtcctcct ccagcgcttg gcggcggctg tcggggagct tctccagctc gctgagcagg
     3361 ctctccaggg cgatccgggc ctggagggcg gcctcccggt cgcggccggc gtcggcgtcc
     3421 gcccgcgcgc gcaggaccag ctcctcgcac gccagcgtgg actcgcggcc gcccagcagt
     3481 gccgcgaacc gctcctcggg ggcctccatc gagcgcttga ccttggcgac gccccggggc
     3541 agctcccagc cttcgtcgaa gcggccctcc gccacctgct cgccggagcc gaaccccagc
     3601 cggaccacca gcgccgaggc ctgggtcacg tcccgcgccg cgccgtcggc gcgggcggtc
     3661 cggtgcgcgt ggagcgcgcg gttgagcacc accaccgccg cggcgatctc ggcctcgcgc
     3721 tcgtcctcgg agtcgcgcag gcgggcaagc cactcctggg cggggccgga gccggcgaag
     3781 gcctcgggga ggatgaccgt ggcccgggag gtgggcaccg gctcggcgac ggcctgctcg
     3841 acctcggtgc cgcggcgccc ccgcatgagc cggcgctggg gcgcgcccag cgtgcgcagc
     3901 gccagcacgc gctctacctg ctcggctccg ggcgcccgca ccaggtagcg gccgtcggcc
     3961 gggcccagca ggtagccgaa c
//
LOCUS       DASUPW010000087         4119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_18589, whole genome shotgun sequence.
ACCESSION   DASUPW010000087 DASUPW010000000
VERSION     DASUPW010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18589"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VFY44_02040"
     CDS             complement(<1..505)
                     /locus_tag="VFY44_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909242.1"
                     /translation="MTAVTEKAPGKVNLVLRVGDPGPDGLHPLCSLFARLELADTVTV
                     EPAERDEVLCPGVEGDNLAATAVRALREAAGPGTVPPLRVTIDKRLPVAAGLAGGSAD
                     AAATLRAANAVAGDPLDADGLRAVAHGLGSDVPSQVEPASAVVHGTGERIDPVTLAPM
                     GLVLLTDP"
     gene            complement(502..1341)
                     /gene="rsmA"
                     /locus_tag="VFY44_02045"
     CDS             complement(502..1341)
                     /gene="rsmA"
                     /locus_tag="VFY44_02045"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX5909243.1"
                     /translation="MTVADELPRQASLNRLRRFDIRPNRELGQNFLIDDNVLRVIEKA
                     AALDPADVVLEVGGGLGVLSEHLAPRVGHLHVVEIDRSLEAALRDAVDPLPNATLHMA
                     DAVQMDLSALDPAPTKVVANLPYGVAATVLLRTVEELPGATLWLAMVQREVAERLAAT
                     PGGKVYGATSVLAQVAGTVKVARKIPRSVFHPVPNVDSALVSIQRTGPSASPEVAALV
                     HAAFAHRRKTIAGSLALVPGADHEIRGRARAALEQLGHPPDARAERLSAADFLRFAEL
                     IRP"
     gene            complement(1338..2087)
                     /locus_tag="VFY44_02050"
     CDS             complement(1338..2087)
                     /locus_tag="VFY44_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX5909244.1"
                     /translation="MVDTHCHLDSCKDDDAELIARARQVGVTRLATIGMDGPTIERAL
                     ATARAHEGVVVVAGRHPHKSEGFGDADAEAIEQAAADPLVRAVGETGLDYFRDYAPRE
                     DQRRAFEAQLDIAARLGLPVVIHTRAAEDDTFAILAERAADLTVLLHCFSAPDRLEEV
                     LERGYLCSFAGNVTYPKNEDLQEAARTLPGDRILVETDSPYLTPVPLRGKPNEPANVV
                     HTAEFVAELRGVSYADLERQVEDNARQLLRW"
     gene            complement(2094..3614)
                     /gene="metG"
                     /locus_tag="VFY44_02055"
     CDS             complement(2094..3614)
                     /gene="metG"
                     /locus_tag="VFY44_02055"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX5909245.1"
                     /translation="MPYYVTTPIYYVNGEPHLGHVYTTVAADVLARHMRQRGEEVFFL
                     TGTDEHGEPVALAAEKLGVTPRELADKNAERFKEVARRVNATNDFFIRTSDPEHAAVV
                     KWVVERIKDNGYVYKDLYEGWYCPRCADFKTENEILEGNRCPIHLIELDRQHEENWFF
                     RLSAFQERLEELYAERPDFVGPSSRYNEALSFIKGGLRDFSLSRSRITWGVPIPWDDE
                     QVVYVWVDALLNYYSALSYAREGQDLTDTFWPATVHVIAKDILKFHAVFWPALLMAAE
                     IEVPQKVFIHGYLLMDEHKMSKSLGNVLDPFEVMDTYGTDALRFYLLREVAFGQDGSI
                     SPAGFEARYNTELANDYGNLASRTLAMIAKYRDGVVPQSTPPEQLVADFEGLADEVAR
                     NLDAVEVSAALEAIWVRVRGLNRFVQDQAPWKMAKEDDRADELDRVLYGLAEGLRVVS
                     VLLLPWLPDTAERLLAALGSDDRSLDCARLGDHGGGSTVGELGQLFPRIEPPAEAA"
     gene            complement(3637..>4119)
                     /locus_tag="VFY44_02060"
     CDS             complement(3637..>4119)
                     /locus_tag="VFY44_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX5909246.1"
                     /translation="PIQVMAHRPGLLNAYGKLEQATAKLDRLPERLNHLAQLKAATLT
                     SCEFCIDLGSQVARKAGLSDQELLALPDYRRSGLFSELDTLVLDYAVAASRTPVDVPD
                     ELFAALRTHLDEAQMVELTHLVALENYRGRFNMAVGIGAAGFSEGMVCAVPEPAVAAA
                     "
BASE COUNT          544 a         1497 c         1484 g          594 t
ORIGIN      
        1 ccgggtcggt cagcagcacc agccccatcg gcgccagcgt gaccgggtcg atccgctcgc
       61 cggtgccgtg gacgaccgcg ctggccggct ccacctggct gggcacgtcg ctgcccaggc
      121 cgtgcgcgac ggcgcgcagg ccgtcggcgt ccaggggatc gccggcgacg gcgttggccg
      181 cccgcagcgt ggccgcggca tcggcgctgc cgccggccag gccggccgcg accggcaggc
      241 gcttgtcgat ggtcacgcgc agcggcggca ccgtgcccgg cccggccgcc tcgcgcagcg
      301 cgcgcaccgc cgtcgccgcc aggttgtcgc cctcgacccc cggacagagc acctcgtcgc
      361 gctcggccgg ctcgacggtg acggtgtcgg ccagctcgag ccgggcgaac agcgagcaca
      421 gcgggtgcag gccgtccggc ccggggtcgc ccacgcgcag gaccaggttg accttgcccg
      481 gtgccttctc ggtgaccgcg gtcacggccg gatcagctcc gcgaaccgca ggaagtcggc
      541 tgcgctcagg cgttcggcac gggcgtccgg cgggtggccc agctgctcga gcgcggcgcg
      601 ggcccggccc cgtatctcgt ggtcggcacc ggggaccagc gccagcgagc ccgcgatggt
      661 cttgcgccgg tgcgcgaacg cggcgtgcac cagcgccgcc acctccggcg aagcggacgg
      721 ccccgtgcgc tggatggaca ccagcgccga gtccacgttg ggcaccgggt ggaagaccga
      781 tcgcgggatc ttgcgcgcga ccttgaccgt gcccgccacc tgcgccagca ccgacgtggc
      841 gccgtacacc ttgccgccgg gcgtcgccgc cagccgctcg gccacctcgc gctggaccat
      901 ggccagccac aacgtggcgc cgggcagctc ctcgaccgtg cgcagcagca ccgtggccgc
      961 caccccgtag ggcaggttgg ccaccacctt cgtcggggcc gggtccagcg cggacaggtc
     1021 catctggacg gcgtcggcca tgtgcagcgt ggcgttgggc agggggtcga ccgcgtcccg
     1081 cagcgccgcc tccagcgacc ggtcgatctc gaccacgtgc aggtgcccga cgcgcggcgc
     1141 caggtgctcg gacaggacgc cgaggccccc gcccacctcc agcaccacgt cggcggggtc
     1201 cagcgccgcc gccttctcga tcacccgcag cacgttgtcg tcgatcagga agttctggcc
     1261 cagctcgcgg ttgggccgga tgtcgaagcg gcgcaggcgg ttcaggctgg cctggcgcgg
     1321 cagctcgtcc gccacggtca ccagcgcagc agctgccgcg cgttgtcctc gacctggcgc
     1381 tcgaggtcgg cgtaggagac gccgcgcagc tcggcgacga actcggccgt gtgcaccacg
     1441 ttggccggct cgttgggctt gccccgcagc ggcaccgggg tcaggtaggg ggagtcggtc
     1501 tcgaccagga tgcggtcgcc gggcagggtg cgcgcggcct cctggaggtc ctcgttcttc
     1561 gggtaggtca cgttgccggc gaacgagcac aggtacccgc gctcgagcac ctcctcaagc
     1621 cggtcggggg ccgagaagca gtgcaggagc accgtcaggt cggcggcccg ctcggccagg
     1681 atcgcgaacg tgtcgtcctc ggccgcacgg gtgtggatga ccaccggcag gcccagccgc
     1741 gcggcgatgt ccagctgggc ctcgaacgcc cgccgctggt cctcacgcgg cgcgtagtcg
     1801 cggaagtagt ccaggccggt ctcgccgacg gcgcggacca gcgggtccgc ggcggcctgc
     1861 tcgatggcct cggcatcggc gtcgccgaag ccctcgctct tgtgcggatg gcggccggcc
     1921 accaccacga cgccctcgtg ggcgcgggcg gtcgccagcg cacgctcgat cgtcggcccg
     1981 tccatgccga tcgtggccag cctggtgacc ccgacctgac gcgcgcgggc gatcagctcg
     2041 gcgtcgtcgt ccttgcagga gtccaggtgg cagtgcgtgt cgaccatgac cgcctacgcg
     2101 gcttcggccg ggggctcgat gcgcgggaac agctgtccga gctcgcccac cgtcgacccg
     2161 ccgccgtggt cgcccaggcg cgcgcagtcc agcgaccggt cgtcgctgcc cagcgccgcc
     2221 agcagccgct cggcggtgtc gggcagccag ggcagcagca ggacgctgac cacgcgcagc
     2281 ccctccgcga gcccgtacag cacccggtcc agctcgtccg cgcggtcgtc ctccttggcc
     2341 atcttccacg gcgcctggtc ctggacgaag cggttgagcc cgcgcacccg cacccagatg
     2401 gcctcgagcg cggcgctcac ctcgaccgcg tccaggttgc gggccacctc gtcggccagg
     2461 ccctcgaagt ccgcgaccag ctgctcgggc ggcgtgctct ggggcacgac cccgtcgcgg
     2521 tacttggcga tcatggccag ggtgcggctg gccaggttgc cgtagtcgtt ggccagctcg
     2581 gtgttgtagc gcgcctcgaa gcccgcgggc gagatcgatc cgtcctggcc gaaggcgacc
     2641 tcgcgcagca ggtagaagcg cagcgcgtcg gtgccgtagg tgtccatgac ctcgaacggg
     2701 tccagcacgt tgcccagcga cttggacatc ttgtgctcgt ccatgagcag gtagccgtgg
     2761 atgaagacct tctgcggcac ctcgatctcc gccgccatca gcagggcggg ccagaacacc
     2821 gcgtggaact tgaggatgtc cttggcgatc acgtgcaccg tggccggcca gaacgtgtcc
     2881 gtcaggtcct ggccctcacg cgcgtacgac agcgccgagt agtagttcag gagggcgtcc
     2941 acccagacgt agaccacctg ctcgtcgtcc cacgggatcg gcacccccca ggtgatgcgc
     3001 gagcgcgaga gcgagaagtc gcgcaggccg cccttgatga acgacagcgc ctcgttgtag
     3061 cgcgagctgg ggccgacgaa gtcgggccgc tcggcgtaca gctcctccag gcgctcctgg
     3121 aaggccgaga gccggaagaa ccagttctcc tcgtgctggc ggtcgagctc gatcaggtgg
     3181 atggggcagc ggttgccctc caggatctcg ttctcggtct tgaagtcggc acagcggggg
     3241 cagtaccagc cctcgtagag gtccttgtag acgtagccgt tgtccttgat ccgctcgacc
     3301 acccacttga ccacggcggc gtgctcgggg tcgctggtgc ggatgaagaa gtcgttggtg
     3361 gcgttcaccc gccgcgccac ctccttgaag cgctcggcgt tcttgtccgc cagctcccgc
     3421 ggcgtcacgc ccagcttctc ggccgccagc gcaaccggct cgccgtgctc gtcggtcccg
     3481 gtcaggaaga agacctcctc cccgcgctgg cgcatgtgcc gggccaggac gtcggcggcg
     3541 acggtggtgt acacgtgccc caggtggggc tcgccgttca cgtagtagat cggggtggtg
     3601 acgtagtagg gcacgacctc ccgatgctac ttgggctcag gcggcggcca ccgcgggctc
     3661 gggcaccgcg cagaccatgc cctcgctgaa cccggccgcg ccgatgccca cggccatgtt
     3721 gaaccgcccg cggtagttct ccagcgcgac caggtgggtc agctccacca tctgggcctc
     3781 gtccaggtgg gtgcgcagcg cggcgaacag ctcgtccggg acgtccaccg gcgtacggct
     3841 ggccgcgacc gcgtagtcca gcaccagcgt gtcgagctcg ctgaacaggc cgctgcgccg
     3901 gtagtcgggc agggccagca gctcctggtc gctgagcccc gccttgcgcg cgacctgcga
     3961 accgaggtcg atgcagaact cgcagctggt cagcgtggcc gccttgagct gggccaggtg
     4021 gttgagccgc tcgggcaggc ggtcgagctt ggcggtggcc tgctcgagct tgccgtaggc
     4081 gttgagcagg ccgggccggt gcgccatgac ctggatcgg
//
LOCUS       DASUPW010000088         8986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_158118, whole genome shotgun sequence.
ACCESSION   DASUPW010000088 DASUPW010000000
VERSION     DASUPW010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8986
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158118"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2388
                     /locus_tag="VFY44_02065"
     CDS             <1..2388
                     /locus_tag="VFY44_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5909247.1"
                     /translation="PAAPVMLVVSGRQAQVPASLDAALAAADTRLATHLLRVGALDER
                     QAAQMLEGQVDPALHRSVFEAGGGNPFFMEQLARTAGADGGDRPSGPAPHRLEVPPAV
                     TAALAREIAALPPDVRTIVQASAVAGEPFEPDLVAEIAGTAEAAVLAAFDDLQDSELV
                     VPAGLPRRFVFRHPLVRRAVYEGARPGWRLAAHGRAAEALDRRGAPASERAHHIEHAA
                     ARGDLEAVAVLREAAAEVAPRTPGSAALWLQAALRLLPQGAEHDPGRRDALVQLASAL
                     RGSGDLEGCREALRDALDLVDPADLPERARLEAVCATVEAWLGRAEDSHRRLVRVREA
                     LPADDSAEAAMLDIRLAFDALNRFGYSEGADLARRALATAQGLDEPGLIAEAAAGLCL
                     ACGLAGEVAEARTHHELAVSALEGLDESEQAERLEIFFYLSWAQNYVDEMERAIATAE
                     RGLALCRATGQGHLLVPLMLARSLPLDVLGRLGESIAGGEEAIAAARTSPNPQYLFWA
                     QWECGYTHALAGNVERAMALLEESMEISRGLAPNFLSGGQPGSCYGVALVWDGQVERG
                     YDVSIEALGGVELPLIAPYEQVLAHAQLTEALLMLGRLDEAEEHVRRADAVAERLGLV
                     SGRVLADQARASLLAARGLAAEALAVATPARTTADERGLRLDAALLRRIEGRALAALG
                     RREEAVEALQAAESAFDSFPAVRMRDGVRQELRKLGARVTRRGPAPAADQGLEALSAR
                     EREVAELVADRRMNKEIAATLFLSEKTVESHLRSIFRKLSVTSRVEVARLIEPPR"
     gene            complement(2395..3225)
                     /locus_tag="VFY44_02070"
     CDS             complement(2395..3225)
                     /locus_tag="VFY44_02070"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5909248.1"
                     /translation="MTKRNDYRAGVPCFVDTGRVDSAAARDFYGGLFGWEFENVSPGG
                     DAPPYHMAKLGGLTAAGIGTQPEQDWDPVWNTYVSVQDADATAAAVADAGGEVLMAPF
                     DIGPAGRMAVFADPQGAEFCVWQAGATAGSEVVNDVGAVVFNTLHTTDLPAAVEFYGT
                     VFGWAMEAEGEQSWMIRLPGYHEDQDALVPGFTKGMEEMGAPEGFADVVAAVAPDGPA
                     RWEVTFAVESADAAAEKVRELGGSVVSEPQDLPWVRETTVRDPDGAQFVLSQFVPPES
                     "
     gene            complement(3265..3954)
                     /locus_tag="VFY44_02075"
     CDS             complement(3265..3954)
                     /locus_tag="VFY44_02075"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEX5909249.1"
                     /translation="MRLVLWDIDGTLVDTSGHGRDAFVDAFGQVLGRPATLGGITMSG
                     RTDHSIALELLEANGEPADDERLPEIFSALNGALDARSEGIARDGRVMPGVPEVIAAL
                     AGREGVTQSLLTGNIEPNAHVKLAALGLNGLLDMDIGGYGSDSGIRPELVDVARRKAR
                     ELRGIEVERAATVLIGDTPLDVHAAHENGARAVAVATGRFTVQELLDTGADAVLPDVS
                     DVDAAVAAILG"
     gene            4099..6105
                     /locus_tag="VFY44_02080"
     CDS             4099..6105
                     /locus_tag="VFY44_02080"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX5909250.1"
                     /translation="MAELRAAARAAGEAVDDVDYLQSAVRQLSDLVGSRMCSLLVLRD
                     GRLSHGGSVGIPSAFMDAIDGAAIGPHVGTCGAAAFRARPMVTTDIREDPKWEQFRAP
                     AAAAGLQSCWSVPLRLPGGPVLGTFAAYDDRPGAPDPGQLELAEAYASLVALGLDRIG
                     REARLSESYEAVVVALSSALDVRDEYTGEHSAETARMALEVGCRMGLGKADLQRLEQA
                     AMLHDIGKLGIPTEILHAPRALTDEERTVMEQHPVIGERILQGIPYLEEVARAVRHEH
                     ERWDGTGYPDRLAGEAIPLASRIVFACDAWHAMTSDRPYRKALPEHTAREELAANAGT
                     QFDPRAVEAVLAVVGERSGEPAPPIAGQVDAESEENHRNQQLASVAEQTGADDLFVFR
                     LTSEGRYSHVDGTGRGAGWAGNVELDAEHEPAFAEAVRGGRPVCLAHDEPGRVFGPYW
                     ARTAALVPHGPEAVVVLGSAGDHLAGLCEEDLVTQTERAVQATRSVPPVKQLADELEV
                     LEAVRAVTAIQAETVEDALNQVAEVVARSLSCEYGAVRVVDGQGEMRVGEASMGWSPV
                     GGQTVESITEAAEHRLEFPLLIQDMEADPRLGGTPGLEGVTALYAVEFGTPPLGAIAA
                     VHADTSPRGFTLLCRRLTRSVAEAAEVVIRRALAAERLAESRNP"
     gene            complement(6067..6708)
                     /locus_tag="VFY44_02085"
     CDS             complement(6067..6708)
                     /locus_tag="VFY44_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX5909251.1"
                     /translation="MPRGGNHTGLLVDFGGVLTTNVFDSFRLFCRSEGLDADAFLTLM
                     SEDKEARRQLRRVETGDIGEDEFGVILAEKLGVSDSENLIDRLFAGMQADQPMIDAVK
                     KAKAAGIRTGLVSNSIGAGRYDRSKFPEMYDGVVISAEVGLHKPQPEIFLLGAERAGV
                     EPQECVFVDDLRENCQGAEAVGMTAVLHRGAETTLPELERLLGVPGLGQPLGG"
     gene            complement(6752..7825)
                     /locus_tag="VFY44_02090"
     CDS             complement(6752..7825)
                     /locus_tag="VFY44_02090"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX5909252.1"
                     /translation="MEIVDTAAEAAELERPPLLVREPLAAFLDQNGLGSGEIEADRIG
                     EGHSNVTYLIRRGDDRMVLRRPPRPPLPPSAHDVLREARLLSALEGTGARTPRVLATC
                     DDEDVLGGVPFYVMEEAHGSVITTDVPEALDVPEHRRALGFELVDALAEIHAVDWQAA
                     GLEGFGKPTGYLERQLRRFNGLWEHNKTRELPVVSEVGQWLADNLPESGPSTIVHGDY
                     RLGNVMVADEPPARVVSVFDWELATIGDPLADVGYMTITWAQADDPEDLAFGALSAAT
                     RREGFPTRDELVARYEERSGRSVESLAWYQALAIWKAAVFMEGNYKAYAEGRSDDEYL
                     GLFDEGVPALAAQAKQIADTGSR"
     gene            7925..8944
                     /locus_tag="VFY44_02095"
     CDS             7925..8944
                     /locus_tag="VFY44_02095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5909253.1"
                     /translation="MNRTRKLGLSIILVAFMAVGLAACGGADDTAGGSSDSGGEGGGD
                     QLSLVAFSTPEVVYDELTPAFQKTTGGKGVTFKSSFGASGEQSRAVEGGLKADVVNFS
                     LAPDVDKLVDAGLVSKDWTKATPYDGFVSTSLVSFIVRKGNPKGIKGWDDLIKDDVKV
                     VTPNPFTSGAAKWNLLGGFQHGGLDYVEKLIKDHVPVQPKSGREALQAFTSGQGDVLI
                     SYEYEYTTAVKKGEKDLELVVPEDTLLIQNPIAVTKDAKPAAKKFVDYTLSPEGQQFF
                     ADWGYRPVNPETLKKNKSKFPDPKKVTTIAKLGGWPKLNDELFDPENGKIAKIEEDAG
                     VSTAK"
BASE COUNT         1198 a         3247 c         3329 g         1212 t
ORIGIN      
        1 ccggccgcgc cggtgatgct ggtggtctcg gggcgccagg cccaggtgcc ggcgtcgctg
       61 gacgcggcac tggcggcggc cgacacccgg ctcgccaccc acctgctgcg cgtgggtgcg
      121 ctggacgagc ggcaggcggc ccaaatgctg gaggggcagg tggacccggc cctgcaccgg
      181 tccgttttcg aggccggtgg cggcaacccg ttcttcatgg agcagctggc ccggaccgcc
      241 ggtgccgacg gcggggaccg cccgagcggc ccggcgccgc accggctgga ggtgccgccg
      301 gcggtgacgg ccgcgctggc gcgggagatc gccgccctgc ccccggacgt gcgcacgatc
      361 gtgcaggcgt cggccgtggc gggcgagccg ttcgagcccg acctggtggc cgagatcgcc
      421 gggaccgcgg aggccgcggt gctggccgcg ttcgacgacc tgcaggactc ggagctggtg
      481 gtgcccgccg ggttgccgcg ccggttcgtg ttccgccatc cgctggtgcg ccgcgcggtg
      541 tacgagggag cccggccggg ttggaggctc gcggcgcacg ggagggcggc cgaggccctg
      601 gaccgccgcg gcgctcccgc gtccgaacgg gcccaccaca tcgagcacgc cgcggcacgc
      661 ggcgacctgg aggccgtcgc tgtgctgcgg gaggcggccg ccgaggtggc cccgcgcacc
      721 cccggctcgg ccgcgctgtg gctccaggcg gccctgcgcc tgctcccgca gggcgccgag
      781 cacgatcccg ggcggcgcga cgcgctggtg cagctggcct cggccctccg tggcagcggc
      841 gacctggagg gctgccgcga ggcgctgcgg gacgcgctcg acctggtgga cccggcggac
      901 ctgccggagc gggcgcggct ggaggccgtg tgcgccaccg tcgaggcctg gctgggccgg
      961 gccgaggact cgcaccgccg cctcgtgcgc gtgcgcgagg ccctgccggc ggacgactcg
     1021 gccgaggcgg ccatgctgga catccgcctg gccttcgacg cgctgaaccg gttcgggtac
     1081 tccgagggcg cggacctggc ccgccgggcg ctggccacgg cgcagggcct ggacgagccc
     1141 gggctgatcg ccgaggcggc cgccggcctg tgcctggcct gcgggctggc cggcgaggtc
     1201 gccgaggcgc gcacccatca cgagctggcg gtctcggcgc tggaagggct ggacgagtcc
     1261 gagcaggccg agcggctgga gatcttcttc tacctgtcgt gggcgcagaa ctacgtggac
     1321 gagatggagc gcgcgatcgc caccgcggag cgggggctgg cgctgtgtcg cgccaccggt
     1381 caggggcatc tgctggtgcc gctgatgctg gcccggtcgc tgccgctgga cgtgctcggt
     1441 cgcctgggcg agtcgatcgc gggcggcgag gaggccatcg ccgcggcgcg cacgtcaccc
     1501 aacccgcagt acctgttctg ggcccagtgg gagtgcggct acacgcacgc cctggcgggc
     1561 aacgtggagc gggcgatggc gttgctggag gagagcatgg agatctcgcg cggcctggcg
     1621 cccaacttcc tctccggtgg ccagcccggc tcgtgctacg gcgtggcgct ggtctgggac
     1681 ggccaggtcg agcgcggcta cgacgtgtcg atcgaggccc tgggcggggt cgagctaccg
     1741 ctgatcgcgc cctacgagca ggtgctggcc cacgcgcagc tgaccgaggc gctgttgatg
     1801 ctgggccgcc tggacgaggc ggaggagcac gtccggcgcg ccgacgccgt ggccgagcgg
     1861 ctgggcctgg tcagcggccg ggtgctggcc gaccaggcgc gcgcctccct gttggcggcc
     1921 cggggcctgg cagccgaggc gctggcggtg gccacaccgg cgcggacgac cgccgacgag
     1981 cgcgggctgc gcctggacgc cgcgctgctg cgccggatcg agggccgggc gctggctgct
     2041 ctgggtcggc gcgaggaggc cgtggaggcg ctgcaggcgg cggagtcggc gttcgactcg
     2101 ttcccggcgg tccggatgcg cgacggcgtg cgccaggagc tgcgcaagct gggcgcccgc
     2161 gtcacccgcc gcgggcccgc gcccgccgcg gaccagggcc tcgaagccct gtccgcgcgc
     2221 gagcgcgagg tggcggagct ggtggccgac cgccgcatga acaaggagat cgccgccacc
     2281 ctgttcctca gcgagaagac cgtggagtcg cacctgcgca gcatcttccg caagctctcg
     2341 gtgacctccc gcgtcgaggt cgcccgcttg atcgagccac cccgctgagg cccgtcagct
     2401 ctcggggggc acgaactgcg agagcacgaa ctgggccccg tcgggatcgc ggaccgtggt
     2461 ctcgcgcacc cacgggaggt cctgcggctc ggacaccacg ctgccaccca gctcgcgcac
     2521 cttctcggcc gcggcatcgg cgctctccac ggcgaacgtg acctcccagc gggcggggcc
     2581 gtcgggggcc accgcggcca ccacgtcggc gaacccctcg ggcgcgccca tctcctccat
     2641 gcccttggtg aacccgggaa ccagcgcgtc ctggtcctcg tggtagccgg gcaggcggat
     2701 catccacgac tgctcgccct cggcctccat cgcccagccg aacaccgtgc cgtagaactc
     2761 gaccgccgcg ggaaggtcgg tggtgtgcag ggtgttgaac accaccgcac cgacgtcgtt
     2821 cacgacctcc gatcccgccg tggcgcccgc ctgccagacg cagaactcgg cgccctgcgg
     2881 atcggcgaac accgccatgc gcccggccgg gccgatgtcg aacggcgcca taagcacctc
     2941 gcccccggcg tcggccaccg cggcggcggt ggcgtccgcg tcctggacgc tcacgtaggt
     3001 gttccagacc gggtcccagt cctgctcggg ctgggtgccg atccccgccg cggtcaggcc
     3061 cccgagcttg gccatgtggt acggcggggc gtccccgccg ggcgagacgt tctcgaactc
     3121 ccacccgaag agcccgccgt agaagtcgcg tgccgccgcc gagtcgaccc gtccggtgtc
     3181 gacgaagcag ggcacgcccg cccgatagtc gttgcgcttg gtcatgggcc gagcctacgg
     3241 cgggtctagg ccaggatctg tccgctatcc gaggatcgcg gccacggccg cgtcgacgtc
     3301 ggacacgtcg ggcaggaccg cgtccgcgcc ggtgtccagc agctcctgca cggtgaagcg
     3361 gccggtggcc acggccacgg cgcgggcgcc gttctcgtgg gccgcgtgca cgtccagcgg
     3421 tgtgtcgccg atcagcaccg tggcggcgcg ctcgacctcg atcccgcgca gctcgcgggc
     3481 cttgcggcgg gcgacgtcga ccagctcggg gcggatgccg ctgtccgagc cgtagccgcc
     3541 gatgtccatg tccagcagcc cgttcagacc cagcgccgcc agcttcacgt gggcgttggg
     3601 ctcgatgttg ccggtcagca gcgactgcgt gactccctcc cgtccggcca gcgcggcgat
     3661 gacctcgggc accccgggca tcacgcggcc gtcacgagcg atgccctcgc tgcgggcgtc
     3721 cagggcgccg ttcagcgccg agaagatctc cggcagccgc tcgtcgtcgg ccggctcccc
     3781 gttggcctcg agcagctcga gcgcgatcga gtggtcggtg cgcccggaca tggtgatgcc
     3841 gcccagcgtg gccggccggc ccagcacctg gccgaacgcg tccacgaacg cgtctcggcc
     3901 gtgacccgag gtgtccacca gggtgccgtc gatgtcccag aggaccagtc gcaccggatc
     3961 tgtatacatg cccgcggccg atgcgggtac accgcctgct caagggccgg tacccggcca
     4021 ccgatagatg cagtgacggc cccccgtggg gcgcaggcca tggcttcgaa catcgtgaga
     4081 cccccggacc agttccccgt ggcagagctg cgcgcggccg cccgggcggc gggcgaggcg
     4141 gtcgacgacg tcgactacct gcagagcgcg gtccgccagc tcagcgacct ggtgggcagc
     4201 cggatgtgct cgctgctggt gctgcgcgac ggccgcctgt cgcacggcgg gtcggtcggg
     4261 atcccgtccg cgttcatgga cgccatcgac ggagcggcga tcggcccgca cgtcgggacc
     4321 tgcggcgcgg ccgccttccg tgcccgcccg atggtgacca ccgacatccg cgaggacccg
     4381 aaatgggagc agttccgggc cccggcggcg gccgccgggc tgcagtcgtg ctggtcggtg
     4441 ccgctgcgcc tgccgggcgg gcccgtgctg ggaaccttcg ccgcctacga cgaccgtcca
     4501 ggggccccgg accccggcca gctggagctg gcggaggcgt acgcgtcgct ggtggcgctc
     4561 ggcctggacc ggatcgggcg cgaggcgcgg ctgtccgaga gctacgaggc ggtggtggtg
     4621 gcgctctcgt cggcgctcga cgtccgtgac gagtacacgg gcgagcactc cgccgagacc
     4681 gcgcggatgg cgctcgaggt gggctgcagg atgggcctgg gcaaggccga cctccagcgc
     4741 ctggagcagg cggcgatgct gcacgacatc ggcaagctgg ggatccccac cgagatcctg
     4801 cacgcccccc gggcgctcac cgacgaggag cgcacggtga tggagcagca cccggtcatc
     4861 ggcgagcgca tcctccaggg catcccctac ctcgaggagg tggcccgcgc cgtgcgccac
     4921 gagcacgagc gctgggacgg caccggctac ccggaccggc tggccggaga ggccatcccg
     4981 ctggccagcc ggatcgtctt cgcctgcgac gcctggcacg ccatgacgtc ggaccggccc
     5041 taccgcaagg cgctgcccga gcacacggcc cgtgaggaac tggcggccaa cgccggcacc
     5101 cagttcgacc cgcgcgcggt ggaggccgtc ctggcggtcg tgggcgagcg cagcggggag
     5161 ccggcgccgc cgatcgccgg ccaggtcgac gccgagtccg aggagaacca ccgcaaccag
     5221 cagctggcct ccgtcgccga gcagaccggc gccgacgacc tgttcgtctt ccgcctgaca
     5281 tcggaggggc gctactcgca cgtggacggc accggccgcg gcgccggctg ggccggcaac
     5341 gtggagctgg acgccgagca cgagccggcg ttcgccgagg cggtgcgcgg cggccggccg
     5401 gtgtgcctcg cccacgacga gccggggcgg gtgtttggtc cctactgggc caggaccgcg
     5461 gccctggtcc cgcacgggcc ggaggcggtg gtggtgctgg gcagcgccgg cgaccatctg
     5521 gccggcctct gcgaggagga tctggtcacg cagaccgagc gcgccgtcca ggccacgcgg
     5581 tcggtgccgc ccgtcaagca gctggcggac gagctcgagg tccttgaggc ggtgcgggcg
     5641 gtgaccgcga tccaggcgga gaccgtcgag gacgctctga accaggtggc cgaggtggtc
     5701 gcgcggtcgc tctcgtgcga gtacggcgcc gtgcgcgtgg tcgacggcca gggcgagatg
     5761 cgcgtgggag aggccagcat gggctggtcc cccgtgggcg gccagacggt ggagagcatc
     5821 accgaggcgg cggagcaccg gctggagttc ccgctgctga tccaggacat ggaggccgac
     5881 ccccggctgg gcgggacccc gggcctggag ggcgtgaccg cgctctacgc ggtcgagttc
     5941 ggcaccccgc ccctcggcgc gatcgccgcg gtgcacgccg acacctcgcc gcgcgggttc
     6001 accctgctct gccggcgcct gacccggtcg gtggccgagg ccgccgaggt cgtgatccgg
     6061 cgcgcgctag ccgccgagcg gctggccgag tcccggaacc cctagcagcc gctcgagctc
     6121 gggcagggtg gtctcggcgc cccggtgcag cacggcggtc atgcccacgg cctccgcgcc
     6181 ctggcagttc tcgcgcaggt cgtccacgaa cacgcactcc tgcggctcga cgccggcgcg
     6241 ctcggcgccc aggaggaaga tctccggctg gggcttgtgc aggccgacct cggcggagat
     6301 gaccacgccg tcgtacatct cggggaactt cgagcggtcg tagcggccgg cgccgatcga
     6361 gttcgacacc agcccggtgc ggataccggc cgccttggcc ttcttgaccg cgtcgatcat
     6421 cggctggtcg gcctgcatgc cggcgaacag ccggtcgatc aggttctcgc tgtccgagac
     6481 gccgagcttc tcggccagga tcacgccgaa ctcgtcctcg ccgatgtcgc ccgtctcgac
     6541 ccggcgcagc tggcggcggg cctccttgtc ctcggacatc agcgtcagga acgcgtcggc
     6601 gtccaggccc tcggagcggc agaacagccg gaacgagtcg aacacgttgg tggtcaggac
     6661 cccgccgaag tcgacgagaa ggcccgtgtg attacccccg cgaggcatcc tgcctctcgg
     6721 ttccgaattt cttgctcggc atcacgcctc gctacctgga gccggtgtcc gcgatctgct
     6781 tggcctgcgc cgccagggcg ggcacgccct cgtcgaacag gcccaggtac tcgtcgtcgc
     6841 tgcggccctc ggcgtaggcc ttgtagttgc cctccatgaa caccgcggcc ttccagatgg
     6901 ccagcgcctg gtaccaggcc agcgactcga ccgagcggcc gctgcgctcc tcgtagcgcg
     6961 ccaccagctc gtcgcgggtg gggaagccct cgcgccgggt ggcggccgag agcgcgccga
     7021 aggccaggtc ctcggggtcg tcggcctggg cccaggtgat ggtcatgtag cccacgtcgg
     7081 ccagcgggtc gccgatggtg gccagctccc agtcgaacac cgagacgacg cgggcgggcg
     7141 gctcgtcggc caccatcacg ttgcccagcc ggtagtcgcc gtgcacgatc gtggacggcc
     7201 cggactcggg caggttgtcg gccagccact ggccgacctc gctgaccacc ggcagctcgc
     7261 gggtcttgtt gtgctcccac aggccgttga agcggcgcag ctggcgctcc aggtagccgg
     7321 tcggcttgcc gaagccctcc agccccgcgg cctgccagtc gacggcgtgg atctcggcca
     7381 gggcgtccac cagctcgaag cccagcgccc gccggtgctc gggcacgtcc agcgcctcgg
     7441 gcacgtcggt cgtgatcacc gagccgtgcg cctcctccat gacgtagaag ggcaccccgc
     7501 ccagcacgtc ctcgtcgtca caggtggcca gcacccgcgg cgtgcgcgca ccggtcccct
     7561 ccagggcgct gagcaagcgg gcctcacgca gcacgtcgtg cgcggacggg ggcagcggcg
     7621 ggcgcggcgg ccggcgcagg accatccggt cgtcgccgcg gcggatcagg taggtcacgt
     7681 tggagtggcc ctcgccgatc cggtcggcct ctatctcgcc cgaccccagc ccgttctggt
     7741 ccaggaacgc ggcaagcggc tcgcggacca gcagcggggg gcgttccagt tcggcggcct
     7801 cggcggccgt gtccacgatc tccatcccgc cgcgcatcct tccagacgag gcatctccac
     7861 cttctccacg ggcttgctag ggttccgaat ctcgtaaatc cgcccaactt tgtggagtat
     7921 tgaattgaac cggactcgca agctcggact cagcatcatc ctggtcgcct tcatggccgt
     7981 gggcctggcc gcctgcggcg gcgccgacga caccgccggc ggctcctcgg actcgggtgg
     8041 tgagggcggt ggagaccagc tctcgctggt ggccttctcg acacccgagg tggtctacga
     8101 cgagctgacc ccggccttcc agaagaccac cggcggcaag ggcgtgacct tcaagagctc
     8161 gttcggcgcg tccggcgagc agagccgcgc ggtcgagggc ggcctgaagg ccgacgtcgt
     8221 gaacttctcg ctggcccccg acgtcgacaa gctggtcgac gccggcctgg tgagcaagga
     8281 ctggaccaag gccacgccgt acgacggctt cgtgtccacc tcgctcgtgt cgttcatcgt
     8341 gcgcaagggc aacccgaagg gcatcaaggg ctgggacgac ctgatcaagg acgacgtcaa
     8401 ggtcgtgacc cccaacccgt tcacctcggg cgcagccaag tggaacctgc ttggcggctt
     8461 ccagcacggc gggctcgact acgtcgagaa gctgatcaag gaccacgtgc cggtgcagcc
     8521 caagtcgggc cgcgaggcgc tgcaggcctt caccagcggc cagggcgacg tgctgatctc
     8581 ctacgagtac gagtacacga cggcggtcaa gaagggcgag aaggacctgg agctggtcgt
     8641 ccccgaggac accctcctga tccagaaccc gatcgccgtg accaaggacg ccaagccggc
     8701 ggccaagaag ttcgtggact acacgctcag cccggagggc cagcagttct tcgccgattg
     8761 gggctaccgc ccggtcaacc ccgagacgct gaagaagaac aagtccaagt tcccggatcc
     8821 gaagaaggtc acgaccatcg ccaagctcgg cggctggccc aagctcaacg atgagctgtt
     8881 cgatcccgag aacggcaaga tcgccaagat cgaggaggac gcgggggtgt cgaccgccaa
     8941 gtgagctcgg tcggcgcctc cgtcgcgtcc cgtccgcggc gccggt
//
LOCUS       DASUPW010000089         3122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1959673, whole genome shotgun sequence.
ACCESSION   DASUPW010000089 DASUPW010000000
VERSION     DASUPW010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1959673"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VFY44_02100"
     CDS             <1..192
                     /locus_tag="VFY44_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909254.1"
                     /translation="VLLWIGRTFNNYYLLWPMMGAAVAFLVWAGEREDAAQGAHAPRT
                     PRRPLAGPRELSEPAAPRP"
     gene            complement(164..1510)
                     /locus_tag="VFY44_02105"
     CDS             complement(164..1510)
                     /locus_tag="VFY44_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5909255.1"
                     /translation="MTRKLLAAGAVLLLLVAGAAAYKVLDKEDPPQKRGSATEEFNPT
                     ETAKAPKVPKKKMIAVAWPTFGYDIQRTKESPYDHGPPFKRTWRVDAKDTLEFPASAA
                     YGNIYIAQQKGLFIAMRGKTGKRVFKTKDFKRCAASSPTIAKGVIYQSYMDFVECGQN
                     DPNPTGFVIAMDAKTGKQKWRYKGKPFESSPLLHDGILYVGSWDGKVHAIRAKTGRRV
                     WTHDTGARVNTSAAYSKGRIFVGNQSGSVYALNAKTGRRAWSASQATEFFYATPAVSY
                     GRVFIGSSDGTMYAYGQKSGKLLWAKPLGSYIYASAALYDGKVYTGTYDGKFFALDAG
                     TGDVKWQRGMPSAVHAAPVVMNGLVYAATCGTCGSAAARSVKTGVDSTTAFNAFTGKK
                     VWFTRAGKYASPIVADQDRTYLIGRAHIYAMENSGSRTKAARKERRRARAAARRAR"
     gene            complement(1521..>3122)
                     /locus_tag="VFY44_02110"
     CDS             complement(1521..>3122)
                     /locus_tag="VFY44_02110"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HEX5909256.1"
                     /translation="VDSEGNQVREIFSERRLAGDTKHRFTWDGRDDEGVVVPDGRYRL
                     RVVRRDQAQPLDSIKKIIVDTRPPRVRVAAVRPNVISPGVRGGAKRVRVRYAGPINAA
                     PEFRVWRTDVNGPPAIVRRFRGNAHTGVWDGTVRDGPTRDGSYAFTVVVRDKAGNRTE
                     APSSPIPTLQTARPRTGVDVRRLTLQGPSAPVSAGSLARLRVGPVPRRFRFALSRLGS
                     TAALRQDTRRGARLRVRIPDDARTGVYLVRVRAGGRRAVWPVVVNGRPAGGRRALSRP
                     RPLVILPVATWQGTNQWDSDADGFPDTLSDASSVPVDRPYAGGKVPRGLTAARPLLEF
                     LDRERLSYDLTTDVALARRQGPSLSNAPGVAIAGTARWVPRQLRDGLRREVEDEGLAV
                     ASFGDRSLRGTVAVVGARLENPSPPRPDDLFGERTRTQRLDAAAPLNQQEDRLRVFRG
                     TDRLFGDFSRLEVSTSLPDDAAPLSAAGREEEGRPAFVGYRFGKGTVIRPGTPQWVSQ
                     LSEDALGLEVPRITKNVWRVLRERR"
BASE COUNT          375 a         1118 c         1164 g          465 t
ORIGIN      
        1 gtgctgctgt ggatcggccg cacgttcaac aactactacc tgctgtggcc gatgatgggc
       61 gcggcggtcg cgttcctggt ctgggcgggt gagcgcgagg atgccgcgca gggggctcac
      121 gccccccgca ccccccgtcg acccttggcc ggcccccgcg agctcagcga gcccgccgcg
      181 ccgcggccct agcccggcgc cgctccttgc gcgcggcctt cgtcctcgag ccgctgttct
      241 ccatcgcgta gatgtgggcg cggccgatca ggtaggtgcg gtcctggtcg gccacgatcg
      301 ggctggcgta cttgccggcg cgggtgaacc agaccttctt gccggtgaag gcgttgaacg
      361 cggtcgtgct gtccacgccg gtcttgaccg agcgcgccgc cgccgacccg caggtgccgc
      421 acgtggcggc gtagaccagc ccgttcatga ccaccggcgc ggcgtgcacc gcgctgggca
      481 tcccgcgttg ccacttgacg tcgcccgtgc cggcgtccag cgcgaagaac ttgccgtcgt
      541 aggtgccggt gtagaccttg ccgtcgtaga gcgcggccga ggcgtagatg tagctgccca
      601 gcggcttggc ccacagcagc ttgccgctct tctggccgta cgcgtacatg gtgccgtcgc
      661 tggagccgat gaacacgcgc ccatagctca cggcgggcgt ggcgtagaag aactcggtgg
      721 cctggctggc gctccaggcg cgcctgccgg tcttggcgtt cagcgcgtag acgctgccgc
      781 tctggttgcc cacgaagatc cggcccttgg agtaggcggc cgacgtgttc acgcgcgcgc
      841 cggtgtcgtg ggtccagacc ctgcggccgg tcttcgcccg gatggcgtgc accttgccgt
      901 cccagctgcc cacgtagagg atgccgtcgt gcagcagcgg cgaggactcg aacggcttgc
      961 ccttgtagcg ccacttctgc ttccccgtct tggcgtccat ggcgatcacg aagcccgtgg
     1021 ggttcgggtc gttctggccg cattccacga agtccatgta ggactggtag atcacgccct
     1081 tggcgatggt gggtgacgag gcggcgcagc gcttgaagtc cttggtcttg aacacgcgct
     1141 tgccggtctt gccgcgcatc gcgatgaaca gccccttctg ctgggcgatg tagatgttcc
     1201 cgtaggcggc cgaggccggg aactcgagcg tgtccttcgc gtcgacccgc caggtgcgct
     1261 tgaacggcgg gccgtggtcg tagggcgact ccttggtgcg ctggatgtcg tagccgaacg
     1321 tgggccaggc caccgcgatc atcttcttct tggggacctt cggcgccttt gccgtctcgg
     1381 tcgggttgaa ctcctccgtg gccgagccgc gcttctgtgg cgggtcctcc ttgtccagga
     1441 ccttgtaggc agcggcgccg gccacgagca gcaggaggac cgcgccggcg gccagcagct
     1501 tgcgcgtcat gggcggcacc ctagcggcgc tctcggagca ctcgccacac gtttttcgtg
     1561 atccgcggga cctccagccc gagggcgtcc tcgctcagct gggacaccca ctggggggtg
     1621 ccggggcgga tcacggtgcc cttgccgaag cggtagccga cgaaggccgg gcgcccctcc
     1681 tcctcccggc ccgcggcgct gagcggcgcg gcgtcgtcgg gcagggacgt ggacacctcc
     1741 agccgcgaga agtcgccgaa cagccggtcg gtgcccctga acacgcgcag gcggtcctcc
     1801 tgctggttca gcggagcagc ggcgtccagg cgctgcgttc gggtgcgctc gccgaacagg
     1861 tcgtcggggc gtggcgggct ggggttctcc agccgcgcgc cgaccacggc gaccgtgccg
     1921 cgcagggagc ggtcgccgaa ggacgccacc gcgagcccct cgtcctcgac ctcgcggcgc
     1981 aggccgtcac gcagctggcg gggcacccac cgggcggtgc cggcgatggc cacgcccggc
     2041 gcgttgctca gcgacgggcc ctggcggcgg gccagcgcca cgtccgtggt caggtcgtac
     2101 gagagccgct cgcggtccag gaactccagc agcggtcgcg ccgcggtcag cccgcgcggc
     2161 accttgccgc ccgcgtacgg ccggtcgacc ggcaccgagg aggcgtccga gagcgtgtcc
     2221 gggaagccgt cggcgtccga gtcccactgg ttcgtcccct gccaggtggc gacggggagg
     2281 atcaccagcg ggcgcggccg gctcagggcg cgccggccgc ccgcggggcg gccgttgacc
     2341 accaccggcc agaccgcacg gcggcccccg gcgcgcaccc gcacgaggta gacgccggtg
     2401 cgcgcgtcgt cggggatgcg cacgcgcagg cgggcgccgc ggcgcgtgtc ctggcgcagg
     2461 gccgcggtcg agcccaggcg cgagagggcg aagcggaagc ggcgcggcac cggcccgacg
     2521 cgcagccggg ccagcgatcc cgccgacacc ggcgcggacg gcccctggag cgtcaggcgg
     2581 cgcacgtcga cgccggtgcg cggccgcgcg gtctggagcg tcgggatcgg cgacgacggc
     2641 gcctcggtgc ggttgccggc cttgtcgcgc accacgacgg tgaacgcgta gctgccgtcg
     2701 cgcgtgggcc cgtcgcggac ggtgccgtcc cacaccccgg tgtgggcgtt gccgcggaag
     2761 cggcgcacga tcgcgggcgg cccgttcacg tcggtgcgcc acacgcggaa ctcgggcgcg
     2821 gcgttgatgg ggccggcgta gcggacgcgc acccgcttcg ccccgccgcg cacgcccggg
     2881 gagatcacgt tgggccgcac ggcggccacg cgcaccctgg gcggccgggt gtccacgatg
     2941 atcttcttga tcgagtccag cggctgggcc tggtcgcgcc ggacgacccg caggcggtag
     3001 cgcccgtcgg ggaccaccac gccctcgtcg tcgcgcccgt cccaggtgaa gcggtgcttg
     3061 gtgtcgccgg ccagccgccg ctccgagaag atctcgcgga cctggttgcc ctcggagtcg
     3121 ac
//
LOCUS       DASUPW010000090         4058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1543844, whole genome shotgun sequence.
ACCESSION   DASUPW010000090 DASUPW010000000
VERSION     DASUPW010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543844"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1037)
                     /locus_tag="VFY44_02115"
     CDS             complement(135..1037)
                     /locus_tag="VFY44_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5909257.1"
                     /translation="MPEISIHGRRFAYREAGDKGPTVLLVHGITNSSATWAPVTERLA
                     ARGLHVLAPDMAGHGDSERQRGDHSLGAHASILRDFLAVKEVDRATIVGHSLGGGLVM
                     QFGYQFPEMAERMVLVASGGLGREVSLAIRAATLPFAEDVLGVATSRPVTAAVGAVGG
                     ALGRLGIHPGGDIEEILRGLGSLSDGERRHAFVRTAKSVITPRGQRVAATDKLYLIVD
                     LPTLLVAGRYDKVIPVEHTRAAARLAPGSQLEIFEDSGHFPHLDEPDRFADLVAAFVK
                     ETKPAKYDRATARRRIVEGPPALP"
     gene            1074..2051
                     /locus_tag="VFY44_02120"
     CDS             1074..2051
                     /locus_tag="VFY44_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX5909258.1"
                     /translation="MATPRRAYGKEVWSSPEWRAEATAWLDARLADAGLERTGDVEHL
                     HVRPWATAMRAPTADGFVWMKAAGPATAFEVGLYELLHRVAPDHVLRPIATDTDRAWM
                     VLEDGGPVLTDLMEGEQLVEAMATALGRYAELQRLVAPHAEELVALGASDMRPALLPQ
                     RFERALELVADRDDRARLEALRPDVARWCAELAEAPGGASIDHNDLHPDNVFAGPPPR
                     AYDWGDSVVAHPFATMLTGLGFMRARVLDAPEDDPRVLRLRDAYLDGFTDLAPRAELV
                     RALDLATRLALIAHALTWDEVLGAMPASDVPEGWDAMPASFLMSLARPA"
     gene            complement(2038..2283)
                     /locus_tag="VFY44_02125"
     CDS             complement(2038..2283)
                     /locus_tag="VFY44_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909259.1"
                     /translation="MLQAGWPAAFPVVQFPNAPLAVALLATLVARLASGDVHDYAYAV
                     ASVGFAAWAYLELAAGVNWFRRLLGAAALVWVVLRLV"
     gene            complement(2391..2849)
                     /locus_tag="VFY44_02130"
     CDS             complement(2391..2849)
                     /locus_tag="VFY44_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5909260.1"
                     /translation="MPDSPEPFVVRPGGGQTVQGPAGGPLTFKVRGDQSGGALTVIEN
                     VIAPGDGPPHHVHANEDEAWWIIEGHLRFKLGEREETAEAGAFVYVPRGVAHSFQNPG
                     PDPARILVMFTPGGMEGFFDAFGKLEELDREAFSRLGAEVGMEVVGPPLR"
     gene            complement(2952..3515)
                     /locus_tag="VFY44_02135"
     CDS             complement(2952..3515)
                     /locus_tag="VFY44_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909261.1"
                     /translation="MKRLLVGIPVLAALALPGAVQADSKTDKTNAAQECRAERGTTAA
                     TREAFRVRYGTNANGKNAFGKCVSKRSKAEARQNSAAAVNGAKECKAERTALGEAAFA
                     AKYGTGKKGKNAYGKCVSQKAKAKKDAADTADAKAIAARKSAAKACATERKAGPDAFK
                     AKYGTNANKSNAFGKCVSAGARQKTKS"
     gene            3630..>4058
                     /locus_tag="VFY44_02140"
     CDS             3630..>4058
                     /locus_tag="VFY44_02140"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5909262.1"
                     /translation="MAQALDPAIGTTRTRSPKGLLALRGERHLVARLHVGDEAAFEVL
                     YDRYAPPLLSFCRHMLGRTEEAEDAVQQVFASALSDLQRNQRELELKPWLYAIARNRC
                     LSMLRARREHPDADLEPSTEGLQEQVQTRADLRQLVADLAD"
BASE COUNT          511 a         1481 c         1508 g          558 t
ORIGIN      
        1 cggcgcgggc ggctggccgt ctcgccccgg ctccgagggc ggctcctgcg ccggccctcg
       61 ctggcgctgc cggccacggt gaccgagacc ggcgacgagg cgctggcccg ccgggtcgcc
      121 gtgcgcgtgc gcccctaggg cagcgccggc gggccctcga cgatccggcg ccgggcggtc
      181 gcgcggtcgt acttcgcggg cttggtctcc ttgacgaacg cggccaccag gtcggcgaac
      241 cggtccggct cgtccaggtg cgggaagtgg ccgctgtcct cgaagatctc cagctggctg
      301 ccgggcgcca gccgcgccgc ggcccgggtg tgctcgaccg ggatgacctt gtcgtaccgc
      361 ccggccacca gcagggtggg caggtcgacg atcaggtaga gcttgtcggt ggcggccacg
      421 cgctggccgc gcggcgtgat caccgacttg gccgtgcgca cgaacgcatg gcgacgctcg
      481 ccgtcggaca gcgagcccag gccgcgcagg atctcctcga tgtccccgcc cggatggatg
      541 cccaggcgtc ccagcgcgcc acccaccgcc ccgaccgcgg cggtcacggg ccggctggtg
      601 gccacgccca gcacgtcctc ggcgaacggg agcgtggcgg cccggatggc caggctgact
      661 tcgcgcccca gcccgccgct ggccaccagc accatccgct cggccatctc ggggaactgg
      721 tagccgaact gcatgaccag gccgccgccc agcgagtgcc cgacgatcgt ggcccggtcc
      781 acctccttga cggccaggaa gtcgcgcagg atcgacgcgt gggcgcccag cgagtggtcc
      841 ccgcgctggc gctccgagtc gccgtgcccg gccatgtccg gcgccagcac gtgcaggccg
      901 cgggcggcca gccgctcggt cacgggcgcc caggtggccg agctgttggt gatcccgtgc
      961 accagcagca cggtcggccc cttgtcgcct gcctcgcggt aggcgaagcg gcggccgtgg
     1021 atggagatct cgggcatgcc ccgatgaaac cactccggga tacgtttcgg cccgtggcca
     1081 ccccgcgacg ggcatacggc aaggaggtct ggtcatcgcc ggagtggcgc gcggaggcga
     1141 ccgcgtggct ggacgcgcgc ctggccgacg ccggcctgga gcgcaccggc gacgtcgagc
     1201 acctgcacgt gcggccgtgg gccaccgcga tgcgggcccc caccgcggac gggttcgtgt
     1261 ggatgaaggc cgcgggcccg gcgacggcct tcgaggtcgg cctgtacgag ctgctgcacc
     1321 gggtggcccc cgaccacgtg ttgcggccga tcgccaccga caccgaccgg gcgtggatgg
     1381 tgctggagga cggcgggccg gtgctgaccg acctgatgga gggcgagcag ctggtggagg
     1441 cgatggccac cgccctgggc cgctacgccg agctacagcg gctggtggcg ccgcacgccg
     1501 aggagctggt ggcgctcggc gcctccgaca tgcgcccggc gctgctgccc cagcggttcg
     1561 aacgcgcgct ggagctggtg gccgaccgtg acgaccgggc ccggctcgag gcgctgcgcc
     1621 cggacgtcgc gcgctggtgc gccgaactgg ccgaggcgcc ggggggcgcc agcatcgacc
     1681 acaacgacct gcaccccgac aacgtgttcg ccggcccgcc cccgcgcgcg tacgactggg
     1741 gcgacagcgt ggtggcgcac cccttcgcca cgatgctgac ggggctgggc ttcatgcgcg
     1801 cccgcgtgct ggacgcgccc gaggacgacc cccgcgtgct ccggctccgc gacgcctacc
     1861 tggacggctt caccgacctg gcgccacgcg cggagctggt gcgggcgctg gacctggcca
     1921 cgcggctggc gctgatcgcc cacgcgctga cgtgggacga ggtgctgggc gcgatgcccg
     1981 cctccgacgt gcccgagggc tgggacgcga tgcccgcctc gttcctgatg tcgctggcta
     2041 gaccagcctg aggacgaccc agaccagcgc cgccgcgccc aggaggcggc ggaaccagtt
     2101 cacgcccgcc gccagctcga ggtaggccca ggcggcgaag ccgaccgacg cgaccgcgta
     2161 cgcgtagtcg tgcacgtcgc cggaggccag gcgggccacg agggtggcca ggagcgcgac
     2221 ggccagcggc gcgttgggga actggacgac cgggaaggcg gcgggccatc ccgcctggag
     2281 cacgcgcccg gcgcggcgcc cgcgccgggg gcggccgacc gccggcgatc ggaacagatg
     2341 gggcgggagc ggtttggtcg aggcgatcag gtcaggctag tcggcatcgg tcaccgcagg
     2401 ggcggcccga cgacctccat gcccacctcg gcgcccaggc gcgagaaggc ctcgcggtcg
     2461 agctcctcca gcttgccgaa ggcgtcgaag aacccctcca tgccgcccgg cgtgaacatg
     2521 accaggatcc gggcggggtc gggcccgggg ttctggaagc tgtgggcgac gccccggggc
     2581 acgtagacga acgcgcccgc ctcggcggtc tcctcccgct cgcccagctt gaagcgcagg
     2641 tggccctcga tgatccacca ggcctcgtcc tcgttggcgt gcacgtggtg cggagggccg
     2701 tcaccgggtg cgatcacgtt ctcgatcacg gtgagtgctc cgccgctctg gtcgccgcgc
     2761 accttgaagg tgagcgggcc gcccgcgggg ccctggaccg tctggccgcc accggggcgg
     2821 acgacgaagg gctcgggcga gtcgggcaca tgcagatcct aggacgaaga agctgcccag
     2881 gcccgcgatc catcgcgggc ctgggcgcta ggagcgatgc cgagtccgtc cggcacgcgt
     2941 gggagccgct cctaggactt ggtcttctgg cgggcaccgg ccgacacgca cttgccgaac
     3001 gcgttggact tgttggcgtt ggtgccgtac ttggccttga acgcgtccgg gccggccttg
     3061 cgctcggtgg cgcacgcctt ggcggcggac ttgcgcgcgg cgatcgcctt ggcgtcggcg
     3121 gtgtcggccg cgtccttctt ggccttggcc ttctgggaca cgcacttgcc gtaggcgttc
     3181 ttgcccttct tgccggtgcc gtacttggcg gcgaaggcgg cctcgcccag cgcggtgcgc
     3241 tcggccttgc actccttggc gccgttcacg gcggcagcgc tgttctggcg cgcctcggcc
     3301 ttgctgcgct tggagacaca cttgccgaag gcgttcttgc cgttggcgtt ggtgccgtag
     3361 cgcacgcgga acgcctcacg ggtcgccgcg gtggtgccac gctcggcccg gcactcctgg
     3421 gcggcgttgg tcttgtccgt cttgctgtcg gcctggacgg caccgggcag cgccagcgcg
     3481 gccagtaccg gaatgcctac gagaagtcgc ttcacagtgg gtccttccat tgcggggtta
     3541 ccactgcgtc aacgccgctc cggggccgag gttcgccagt aacggacgaa ctttcgccac
     3601 ggcgcggcgt tgaagaggac aatgtggctg tggcccaggc tctcgaccct gcgatcggca
     3661 ccacccggac ccggtctccg aagggactgc tcgccctgcg cggggagcgt cacctggtcg
     3721 cacggctgca cgtgggcgac gaggccgcgt tcgaggtgct ctacgaccgg tacgccccgc
     3781 cgctgctgtc gttctgccgc cacatgctcg gccgcaccga ggaggccgag gacgcggtcc
     3841 agcaggtgtt cgcctccgcc ctgtccgacc tgcagcgcaa ccagcgcgag ctggagctca
     3901 agccgtggct ctacgccatc gcccgcaacc gctgcctgtc gatgctgcgc gcccggcgcg
     3961 agcaccccga cgccgacctc gagcccagca ccgagggcct ccaggagcag gtccagacgc
     4021 gcgccgacct gcgccagctg gtcgccgacc tggccgac
//
LOCUS       DASUPW010000091         3476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_159411, whole genome shotgun sequence.
ACCESSION   DASUPW010000091 DASUPW010000000
VERSION     DASUPW010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159411"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2104)
                     /locus_tag="VFY44_02145"
     CDS             complement(<1..2104)
                     /locus_tag="VFY44_02145"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5909263.1"
                     /translation="MPETTRAADLLEREEELGALAGRLDEAASGQGGLVVVDGAAGVG
                     KSALLRELVATARDRGALVTTAVGGELEREFPFGMVRQLLEPVVVGAPAKRRQKLLSG
                     SAALAGPVLGGAPDAAAEGGDASFATLHGLYWLVAALAEEQPLVMVMDDCHWSDASSL
                     RFVDFLARRAADLPVLVAIGIRPHEPGAEQALLDGLMDRPETELVRPAALSRTAVDAL
                     VDQQLGDRAEEGTAAAAHETTGGNPLLLRELVRNVEGQPGQITPAAVHEVVPSTVTRS
                     VERRLGRLPADSREVATTLAVLGDRLGPELLPAMTGLGGEELVAAIEELRAAELLAGE
                     PPRYAHPLLRQAVAESLPLVERHRLHRRAAEGLRGRQDMEEQLIVHLLAAPPLGEGWA
                     EQVLRDGARRALAEGAADAAVRRLGRALEEHTGPTDHALLLDLGLAELRQGDPAAIGH
                     LDAAAEAGDPVVAGTAVQAAMALRNLDAITDEHTIAQALRGALDRLGPDAPDGLSHGL
                     LGNLLNTAMLSSNLAGERQAMLAEDRDDPPEPLLALRAFEGACLEAPADEVIELGRQA
                     FASRPLLTLHKLEQPSELVAVAALSVVDGRDVADAALRDAEESARRHGSLIGRGFATY
                     MRAEWGLVFGSAVTVEADAREAHALWQETGLANMTFNSASTLAQALVLMGRLDEAEEL
                     LAPLPLGEVEWVSNIGLIA"
     gene            complement(2155..2784)
                     /locus_tag="VFY44_02150"
     CDS             complement(2155..2784)
                     /locus_tag="VFY44_02150"
                     /inference="COORDINATES: protein motif:HMM:NF020398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1802 family protein"
                     /protein_id="HEX5909264.1"
                     /translation="MPDAFKEWAVTVRALAEGEQLLTLRKGGIREDNKHFEIEHDRFF
                     LYPTFDHQQNDLVRDSHLPELGRALEEGVWPDTEPPARALLQDGGIPQPDRVRIRAWA
                     EVAASYLITDPRAVDALSPYYIWTTDYAEKRLQWKRRHPMHVILLRTYRIPRPVTIKV
                     RPEYQGCRSWIELHRDLPFEGTPVLSDDEFERAAAQIEAIASDAVPVLA"
     gene            complement(2870..>3476)
                     /locus_tag="VFY44_02155"
     CDS             complement(2870..>3476)
                     /locus_tag="VFY44_02155"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5909265.1"
                     /translation="IDMSTVSPAASRGFGERLAERGIAFLDAPVTGSRPKAEDGTLTI
                     MVGGPEEAFERARPLFEAMGKLVLHMGPQGHGSTVKLINNTLAAVNAAALAEGISVGR
                     QAGLDLDKAMQVVASGSGNSTMREIKSGPMIDGEYDPLFKLEHMLKDVRYFLAESREM
                     GVPTGVAETASGLYEEADSEGYGQSDFAAVIEAIVTKSRLK"
BASE COUNT          451 a         1299 c         1255 g          471 t
ORIGIN      
        1 gggcgatcag gccgatgttg ctgacccact cgacctcgcc cagcggcagc ggcgccagca
       61 gctcctcggc ctcgtccagg cggcccatca gcaccagcgc ctgcgccagc gtcgaggccg
      121 agttgaaggt catgttggcc aggccggtct cctgccacag cgcgtgcgcc tcgcgggcgt
      181 cggcctccac cgtcaccgcg gagccgaaga ccaggcccca ctcggcgcgc atgtaggtgg
      241 cgaacccgcg gccgatcagc gacccgtggc ggcgcgcgct ctcctcggcg tcgcgcaggg
      301 ccgcgtcggc gacgtcgcgc ccgtcgacca ccgacagcgc cgcgacggcc accagctcgc
      361 tcggctgctc gagcttgtgc agggtcagca gcggccggga ggcgaacgcc tgccggccca
      421 gctcgatcac ctcgtccgcc ggggcctcca ggcacgcgcc ctcgaaggcc cgcagcgcca
      481 gcagcggctc gggcgggtcg tcgcggtcct cggccagcat cgcctgccgc tcaccggcca
      541 ggttcgagct cagcatcgcg gtgttcagca ggttccccag cagcccgtgg gagaggccgt
      601 cgggcgcgtc ggggcccagc cggtccagcg cgccgcgcag ggcctgggcg atggtgtgct
      661 cgtcggtgat cgcgtccagg ttgcgcaggg ccatggcggc ctgcaccgcg gtgcccgcca
      721 cgaccgggtc cccggcctcg gcggccgcgt ccaggtggcc gatggccgcc gggtcgccct
      781 ggcgcagctc ggccagcccg aggtccagca gcagcgcgtg gtcggtcggg ccggtgtgct
      841 cctcgagcgc gcgccccagg cgccggaccg cggcgtcggc ggcgccctcg gccagcgcac
      901 ggcgggcgcc gtcgcgcagg acctgctccg cccacccctc gcccaggggc ggggcggcca
      961 gcaggtgcac gatcagctgc tcctccatgt cctggcgccc gcgcaggccc tcggcggcgc
     1021 gccggtgcag ccggtggcgc tccaccagcg ggagcgactc ggcgacggcc tggcgcagga
     1081 gcggatgggc gtaccgcggc ggctcgccgg ccagcagctc ggcggcccgc agctcctcga
     1141 tcgcggccac cagctcctca ccgcccagcc cggtcatggc ggggagcagc tccggtccca
     1201 gccggtcgcc cagcacggcc agggtggtgg ccacctcgcg gctgtccgcg ggcaggcggc
     1261 cgaggcgccg ctcgaccgag cgcgtgacgg tggacggcac cacctcgtgg accgcggccg
     1321 gggtgatctg ccccggctgg ccctccacgt tgcgcaccag ctcgcgcagc agcagcgggt
     1381 tgccgccggt ggtctcgtgg gccgcggcgg cggtgccctc ctcggcacgg tctcccagct
     1441 gctggtcgac cagcgcgtcg accgcggtcc gcgacagcgc ggccggccgc accagctcgg
     1501 tctcgggccg gtccatgagc ccgtcgagca gggcctgctc ggcgccgggc tcgtgcgggc
     1561 ggatcccgat ggccaccagc acgggcaggt cggccgcccg ccgggccagg aagtccacga
     1621 accgcagcga cgaggcgtcg gaccagtggc agtcgtccat gaccatcacc agcggctgct
     1681 cctcggccag cgcggccacc agccagtaga ggccgtgcag ggtggcgaag gaggcgtcgc
     1741 ctccctcggc ggccgcgtcg ggcgcgccgc ccagcacggg gccggccagg gcggccgagc
     1801 ccgacagcag cttctgccgg cgcttggcgg gcgcgcccac cacgacgggc tccagcagct
     1861 ggcgcaccat gccgaacggg aactcgcgct ccagctcgcc gcccaccgcg gtggtcacca
     1921 gggcgccgcg gtcacgcgcg gtggccacta gctcgcgcag cagggcggac ttgcccacgc
     1981 cggccgcgcc gtccacgacc acgaggccgc cctgaccgga cgcggcttcg tccaggcgac
     2041 cggccagcgc gccgagttcc tcctcccgct cgaggaggtc cgctgcccgt gtcgtctccg
     2101 gcacgggccc aacgtaccgc cgatccggcg gtacgtcgtc ctacctgagg acgatcaggc
     2161 cagtacggga accgcgtccg aggcgatggc ctcgatctgc gcggcggcgc gctcgaactc
     2221 gtcgtcggag agcaccgggg tgccctcgaa cgggaggtcg cggtgcagct cgatccagga
     2281 gcgacagccc tggtactcgg gccggacctt gatcgtcacc gggcgcggga tgcggtaggt
     2341 gcgcaggagg atcacgtgca tgggatggcg ccgcttccac tgaagccgct tctccgcata
     2401 gtcggtggtc cagatgtagt acggcgacag cgcgtccacg gcacgggggt ccgtgatcag
     2461 gtagctggcc gccacctcgg cccaggcgcg gatgcgcacc cggtcgggct gggggatgcc
     2521 gccgtcctgg agcagcgccc gggcgggcgg ctcggtgtcc ggccacacgc cctcctcgag
     2581 ggcacggccg agctcgggca ggtgggagtc gcgcaccagg tcgttctgct ggtggtcgaa
     2641 cgtcggatac aggaagaacc ggtcgtgctc gatctcgaag tgcttgttgt cctcgcggat
     2701 cccgcccttg cggagcgtga gcagctgctc accctctgcg agggcgcgca cggtcacggc
     2761 ccattccttg aaagcgtctg gcatagaaag aaaaacctcc acccgcgccc agaagattgg
     2821 gtcagtgctg gggcggcacg gggccgttca ctatgcatga ctgcaacgcc tatttcaacc
     2881 tggatttcgt gacaatcgcc tcgatgacgg ccgcgaaatc gctctggccg tagccttcgg
     2941 agtcggcctc ctcgtacagc cccgaggccg tttcggccac ccccgtcgga acccccattt
     3001 ccctgctctc ggcaaggaaa tagcgcacgt ccttgagcat gtgctcgagc ttgaagagcg
     3061 ggtcgtactc gccgtcgatc atgggcccgg acttgatctc gcgcatggtc gaatttcccg
     3121 accccgaggc caccacctgc atcgccttgt ccaggtccag gccggcttgg cggccgacgc
     3181 tgatgccctc ggccagcgcg gcggcgttga ccgcggcgag ggtgttgttg atcagcttga
     3241 ccgtggagcc gtgcccctgg gggcccatgt gcagcaccag cttccccatc gcctcgaaca
     3301 gcggcctggc gcgttcgaac gcctcctcgg ggccgccgac catgatcgtc agcgtgccgt
     3361 cctcggcctt gggccgcgat ccggtcaccg gggcgtccag gaaggcgatg ccgcgctcgg
     3421 ccaggcgctc gccgaagccg cggctggcgg cgggcgagac ggtggacatg tcgatg
//
LOCUS       DASUPW010000092         2908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_437122, whole genome shotgun sequence.
ACCESSION   DASUPW010000092 DASUPW010000000
VERSION     DASUPW010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437122"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2908
                     /locus_tag="VFY44_02160"
     CDS             <1..>2908
                     /locus_tag="VFY44_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909266.1"
                     /translation="LGYTVPPAGMTALTAQDVLAILWQLVELHEGRGDTDDIDHLGNR
                     RVRSVGELIANQFSVGLSRMARLVRERMSIVSDPDKINIDDLVNARTVSAVIQQFFGS
                     SQLSQFMDQTNPLAEMTHKRRLSALGPGGLTRERAGFEVRDVHYSHYGRMCPIETPEG
                     PNIGLINSLTTYARINDLGFIETPYRKVVRALVRYPAEAKLEEAVRLVLGDRAKVFAK
                     KGETIDAARGREIFRAMIVGAELAEDVADWNAVFPRMLAGEIGQTEWEDTLSALPVLA
                     RRGERITEELAERLAAQPINLVRVVSRRAGAAARGVAPEAIRNPMSLPVRVFQPTTAA
                     VLGVPGTVLTAEVAEALFARQSQGLDVQEWGDTPDEVALDYTSGAPALPPEGEVGRAL
                     LGTSGRTTHVTPVVTRITAWLSANEEESARIAQANAPLSSGFTFQNEFVLCRERGDFP
                     LLRPEEIDYMDVAPDQLVSVAAALIPFLEHDDANRALMGSNMQRQAVPLLFPDAPLVG
                     TGLESVIARDSGAVVVARRGGVVQEVTADHILIDAGMEGVGSTDEPLRRLAQFDRYRM
                     KKYWRTNQDTALNQRPLIRKGQIVKKGDVLADGPSTEGGELALGRNLLVAFMPWYGHN
                     FEDAIVLSERLVKDDVYTSIHIQELELQVRDTKRGMEEITREIPNVAEESLVDLDERG
                     VVRIGARVKAGDILVGKITPKGETELSPEEKLLTAIFGEKAKDVKDSSLKVPPGVEGT
                     VIDVKIFSRRIDDPILEKERGQKIGELRAFERSEIQRIGEARDEEIRELIRGREVALF
                     LKKGTVEPFFNEGTQLTDEVVGALDFNDIDLTTLKVTDRPTNELLRRLIDESKRRIER
                     VRQRTESQIDKIFQPDELPPGVVQLVKVYLAEKRKISVGDKMAGRHGNKGIIARIVPE
                     EDMPFLPDGTPVDVCLNPLGVPSRMNVGQILETHLGWAARVLGFEAKTPVFQGAS"
BASE COUNT          478 a          956 c         1089 g          385 t
ORIGIN      
        1 cgctgggcta cacggtgccg ccggcgggga tgacggcgct cacggcgcag gacgtcctgg
       61 ccatcctgtg gcagctggtg gagctgcacg agggccgcgg cgacaccgac gacatcgacc
      121 acctgggcaa ccgccgggtg cgcagcgtcg gcgagctgat cgccaaccag ttctcggtgg
      181 gcctgagccg catggcgcgg ctggtccgcg agcgcatgtc gatcgtgtcg gaccccgaca
      241 agatcaacat cgacgacctg gtgaacgcgc gcacggtgag cgcggtgatc cagcagttct
      301 tcgggtcgtc gcagctgagc cagttcatgg accagaccaa ccccctggcc gagatgacgc
      361 acaagcggcg tctctccgcg ctggggccgg gcggcctgac ccgcgagcgc gccggcttcg
      421 aagtgcgcga cgtgcactac tcgcactacg ggcgcatgtg cccgatcgag acgccggaag
      481 ggcccaacat cggcctgatc aactcgctga ccacgtacgc ccggatcaac gacctgggct
      541 tcatcgagac gccgtaccgc aaggtggtgc gcgcgctggt ccgctacccg gccgaggcca
      601 agctggagga ggccgtgcgc ctggtgctgg gcgaccgcgc caaggtgttc gccaagaagg
      661 gcgagaccat cgacgcggcc cgcgggcgcg agatcttccg cgccatgatc gtgggcgcgg
      721 agctggcgga ggacgtggcc gactggaacg cggtcttccc gcgcatgctg gccggcgaga
      781 tcggccagac ggagtgggag gacacgctct cggcgctgcc ggtgctggcc cgccgcggcg
      841 agcgcatcac cgaggagctg gcggagcgcc tggcggcgca gcccatcaac ctggtgcgcg
      901 tggtttcccg gcgcgcgggc gcggcggcgc gcggcgtggc ccccgaggcc atccgcaacc
      961 ccatgtcgct gccggtgcgc gtgttccagc cgaccacggc ggccgtgctg ggcgtgcccg
     1021 ggacggtgct gacggccgag gtggccgagg cgctgttcgc gcggcagtcg caggggctgg
     1081 acgtgcagga gtggggcgac acgccggacg aggtggcgct ggactacacg tcgggcgcgc
     1141 ccgcgctgcc ccccgagggc gaggtgggcc gcgccctgct gggcaccagc ggccgcacta
     1201 cgcacgtgac ccccgtggtc acgcgcatca cggcgtggct gtcggccaac gaggaagagt
     1261 cggcccggat cgcgcaggcc aacgcgccgc tgtcgagcgg gttcaccttc cagaacgagt
     1321 tcgtcctttg ccgcgagcgg ggtgacttcc cgctgctgcg gccggaggag atcgactaca
     1381 tggacgtggc gccggaccag ctggtctcgg tggccgcggc gctgatcccc ttcctggagc
     1441 acgacgacgc caaccgggcg ctgatgggct ccaacatgca gcgccaggcg gtgccgctcc
     1501 tgttccccga cgcgccgctg gtgggcacgg ggctggagag cgtgatcgcg cgcgactcgg
     1561 gcgccgtggt ggtggcccgg cgcggcggcg tggtgcagga ggtgacggcg gaccacatcc
     1621 tgatcgacgc ggggatggag ggcgtgggct cgacggacga gccgctgcgc cgcctggccc
     1681 agttcgaccg gtaccggatg aagaagtact ggcgcaccaa ccaggacacg gcgctcaacc
     1741 agcgcccgct catccgcaag gggcagatcg tcaagaaggg cgacgtgctg gccgacggcc
     1801 cctccaccga gggcggcgag ctggcgctgg ggcgcaacct gctggtggcg ttcatgccct
     1861 ggtacggcca caacttcgag gacgccatcg tcctttcgga gcgcctggtg aaggacgacg
     1921 tgtacacgtc catccacatc caggagctgg agctgcaggt ccgcgacacc aagcggggca
     1981 tggaggagat cacgcgagag atccccaacg tggccgagga gtcgctggtc gacctggatg
     2041 agcgcggcgt ggtccgcatc ggcgcgcggg tcaaggccgg cgacatcctg gtgggcaaga
     2101 tcacgcccaa gggcgagacg gagctgagcc ccgaagagaa gctgctgacg gccatcttcg
     2161 gcgagaaggc caaggacgtg aaggattcgt cgctcaaggt gccgcccggc gtggagggca
     2221 cggtgatcga cgtgaagatc ttcagccgcc gcatcgacga cccgatcctg gaaaaggagc
     2281 ggggccagaa gatcggcgag ctgcgggcgt tcgagcgctc ggagatccag cgcatcggcg
     2341 aggcgcgcga cgaggagatc cgcgagctca tccgcgggcg cgaggtggcg ctgttcctga
     2401 agaaggggac ggtggagccg ttcttcaacg aggggacgca gctcacggac gaggtcgttg
     2461 gcgcgcttga cttcaacgac atcgacctca ccacgctcaa ggtcaccgac cgcccgacca
     2521 acgagctgct gcgccggctg atcgacgaga gcaagcgccg catcgagcgg gtgcgccagc
     2581 gcacggaaag ccagatcgac aagatcttcc agccggacga gctgcccccg ggcgtcgtgc
     2641 agctggtgaa ggtgtacctg gccgagaagc gcaagatctc ggtgggcgac aagatggccg
     2701 ggcgccacgg caacaagggc atcatcgccc gcatcgtccc cgaggaggac atgccgttcc
     2761 ttccggacgg cacgccggtg gacgtgtgcc tgaacccgct gggcgtgccc agccgcatga
     2821 acgtggggca gatcctggag acccacctgg ggtgggccgc ccgggtgctg ggcttcgagg
     2881 ccaagacccc ggtgttccag ggcgcgtc
//
LOCUS       DASUPW010000093         2952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_991353, whole genome shotgun sequence.
ACCESSION   DASUPW010000093 DASUPW010000000
VERSION     DASUPW010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991353"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..857)
                     /locus_tag="VFY44_02165"
     CDS             complement(174..857)
                     /locus_tag="VFY44_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5909267.1"
                     /translation="MTGSPRVLVVEDDGDIAAVLRRALGMEGYEVRLAGDGEAALSEA
                     AVFSPDAVILDLGLPKLDGIEVSRRLREVGDVPILMLTARDAVDSRVEGLDSGADDYL
                     VKPFERTELLARLRALLRRRPPRGSASLVVGDLQLNPDSREVTRGERQIELTSREFEL
                     LEHLMRNERLVVSRDKLLEEVWEYAPFSETNTVDVFISNLRRKLEAGGEPRLLHTIRG
                     SGYVLRPAQ"
     gene            913..>2952
                     /locus_tag="VFY44_02170"
     CDS             913..>2952
                     /locus_tag="VFY44_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX5909268.1"
                     /translation="MARRKPARKPQTKRRPKPKAKGGLGLPALEQHHRDLIGLGLVAF
                     AAFLAFVLYLGAAGGRVGEAVEDGLRFLMGDAAVLAPPALCAVGVVLVLHESLPSVKP
                     FRAGALCLLLGVTLGLAAGSLGLGPGAHSHAPLLDPGYVSDRGGALGELLYAGSSQLF
                     SDLGSHIIFLFLVTGGVLLLTGASIAGIVARTRDGVSATTQAVRRSTSELTAVLREPA
                     PKPQRMPRQDGPPEPPGVEPVVHATHVEAPALDAEARYPDLFESDDEPGAPDWRDEPD
                     PVDEPEPAEEIEPEEPVVPGAVEEEPGVTAEENPEPVQGQLTPMGNRRTAVTEADDVD
                     YKLPRAAFLSRSKNKPKSTSGADERIGQQLVEALSHFGVESKIVGSVSGPHVTRYELR
                     LAPGIKMSKVSNMRDDLAYALAADDVRILAPIPGKRAVGVEVPNRVRNMVHLGDVAQE
                     SPEGWSPLTVWLGKDIDGRAIGIDLASQPHVLVAGTTGSGKSGCVNAMLSSILMRATP
                     NEVRMVLVDPKQVELNLYEHIPHLLTPVITSPRLAANALQNLIKEMEERYALMSGART
                     RKLEELNRMRVRNGERPLPYILCVIDELADLMMVAPGEVEDSIIRLAQKSRAVGIHLV
                     LATQRPSVDIITGMIKTNVPARIAFAVSSQTDSRVILDQNGAESLLGKGDMLFRPG"
BASE COUNT          442 a         1094 c         1043 g          373 t
ORIGIN      
        1 cgcgcggacg tcgttgtcga agttggcctt cagccgctgc tcggtgaacg cgccgaccac
       61 cacggcgaac agcagcagga tcacgaacgt cagcgccgcc gagaccagcg ccagcttgac
      121 ctggatcggc agccgcaggc gcgtccgcct gatcgcaccg cgggcgcggc cgctcactgc
      181 gcgggcctca gcacgtaccc ggagccacgg atggtgtgca gcaggcgcgg ctcgccgcct
      241 gcctcgagct tgcggcgcag gttcgagatg aacacgtcga cggtgttcgt ctccgagaac
      301 ggcgcgtact cccacacctc ctcgagcagc ttgtcgcgcg agacgaccag ccgctcgttg
      361 cgcatcaggt gctcgagcaa ctcgaactcg cggctggtca gctcgatctg gcgctcaccg
      421 cgcgtgacct cgcggctgtc ggggttgagc tggagatcgc cgaccacgag cgacgcgctg
      481 ccgcgcgggg ggcggcgccg cagcagcgcc cgcagccggg ccaggagctc ggtgcgctcg
      541 aacggcttga ccaggtagtc gtcggcgccg gagtccaggc cctcgacgcg cgagtccacc
      601 gcgtcgcggg ccgtcagcat caggatcggc acgtcgccca cctcgcgcag ccgccgcgag
      661 acctcgatcc cgtccagctt gggaaggccc aggtccagga tcaccgcgtc cggcgagaac
      721 accgcggcct cggacagcgc cgcctcgccg tccccggcca ggcgcacctc gtagccctcc
      781 atccccaggg cacgccggag cacggcggcg atgtcgccgt cgtcctcgac caccagcacc
      841 cgaggcgagc ccgtcaccac acggatggta gggaggggga gcgcacctgg cggcgaatga
      901 cagaagtcag caatggcacg ccgtaagccc gctcgcaagc cccagacgaa gcgccgcccc
      961 aagcccaagg cgaagggcgg cctcggcctg cccgccctgg agcagcacca ccgcgacctg
     1021 atcgggctcg gcctcgtggc gttcgcggcc ttcctggcct tcgtgctcta cctgggcgcg
     1081 gccggcggcc gggtgggcga ggcggtcgag gacggcctgc ggttcctgat gggcgacgcc
     1141 gccgtgctgg ccccgccggc gctgtgcgcg gtgggggtcg tgctggtcct gcacgagtcg
     1201 ctgccctcgg tcaagccctt ccgcgccggc gccctgtgcc tgctgctcgg cgtcacgctg
     1261 ggcctggcgg ccgggagcct gggcctcggg cccggggccc actcgcacgc cccgctgctg
     1321 gaccccggct acgtcagcga ccgcggcgga gcgctcggcg agctgctcta cgccggctcc
     1381 agccagctgt tctccgacct cgggtcgcac atcatcttcc tgttcctggt caccggcggc
     1441 gtgctgctgc tgacgggggc gtccatcgcc ggcatcgtcg cccgcacgcg ggacggggtc
     1501 agcgccacca cccaggcggt caggcgctcc accagcgagc tgaccgcggt cctgcgcgag
     1561 cccgccccca agccgcagcg gatgccgcgc caggacggcc cgcccgagcc gcccggggtg
     1621 gagccggtgg tgcacgccac ccacgtggag gcgcccgcgc tggacgccga ggcccgctac
     1681 ccggacctgt tcgagagcga cgacgagccc ggcgcgccgg actggcgcga cgagcccgac
     1741 cccgtcgacg agcccgagcc ggccgaggag atcgagcccg aggagccggt cgtccccggc
     1801 gccgtcgagg aggagccggg cgtgaccgcc gaggagaacc ccgagccggt ccagggccag
     1861 ctgacgccga tgggcaaccg ccgcaccgcg gtgaccgagg ccgacgacgt cgactacaag
     1921 ctgcccaggg ccgcgttcct cagccggtcg aagaacaagc cgaagtccac gtcgggcgcg
     1981 gacgagcgca tcggccagca gctggtcgag gcgctgagcc acttcggcgt ggagtcgaag
     2041 atcgtgggca gcgtcagcgg cccgcacgtg acccgctacg agctgcgcct ggcgcccggg
     2101 atcaagatgt ccaaggtctc gaacatgcgg gacgacctgg cctacgcgct ggccgcggac
     2161 gacgtgcgca tcctcgcccc gatccccggc aagcgtgccg tgggcgtcga ggtgcccaac
     2221 cgcgtccgca acatggtcca cctgggcgac gtggcgcagg agtcgcccga gggatggtcg
     2281 ccgctgaccg tgtggctggg caaggacatc gacggccgcg cgatcggcat cgacctggcc
     2341 agccagccac acgtgctggt ggccggcacg accgggtcgg gcaagtccgg gtgcgtgaac
     2401 gcgatgctgt cctcgatcct gatgcgggcc acgccgaacg aggtgcggat ggtgctggtg
     2461 gaccccaagc aggtcgagct gaacctgtac gagcacatcc cgcacctgct gaccccggtg
     2521 atcaccagcc cgcgcctggc cgccaacgcg ctccagaacc tgatcaagga gatggaggag
     2581 cgctacgcgc tcatgagcgg cgcccgcacg cgcaagctcg aggagctgaa ccggatgcgg
     2641 gtgcgcaacg gcgagcggcc gctgccctac atcctgtgcg tcatcgacga gctggccgac
     2701 ttgatgatgg tcgcgcccgg cgaggtcgag gactcgatca tccggctggc gcagaagtcg
     2761 cgcgccgtgg gcatccacct cgtgctcgcg acgcagcgcc ccagcgtgga catcatcacc
     2821 ggcatgatca agacgaacgt gcccgcgcgg atcgcgttcg ccgtctcgtc ccagaccgac
     2881 tcgcgcgtga tcctggacca gaacggcgcc gagtcgctcc tgggcaaggg cgacatgctc
     2941 ttccgccccg gc
//
LOCUS       DASUPW010000094         2439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_991952, whole genome shotgun sequence.
ACCESSION   DASUPW010000094 DASUPW010000000
VERSION     DASUPW010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991952"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VFY44_02175"
     CDS             complement(<1..532)
                     /locus_tag="VFY44_02175"
                     /inference="COORDINATES: protein motif:HMM:NF015666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909269.1"
                     /translation="MSAPNEPIGVIGVGWVGLCTAAAFAELGHHVYARDILPEKVEAL
                     SRGEVTIHEPGLPELLAKNAERLHFTTDMAEVLDNAELLFCCVDTPPTYSGDADLSRV
                     EAVIAELGDSTDHAIVMKSTVPVGTGRSIARRRADLGYISNPEFLKEGSAVQDFMNPD
                     RVVIGADDGSDDFADRV"
     gene            complement(561..2063)
                     /locus_tag="VFY44_02180"
     CDS             complement(561..2063)
                     /locus_tag="VFY44_02180"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX5909270.1"
                     /translation="MSPGFEDLPRPGIWKKLILGCFLVVFATAGATSVAAFHEVDNVV
                     DTLKTGKDIRLGTGDIAEVDPGEPQTILLLGSDRRPDNAKDGGAGTGARSDTIILARL
                     DPDRKATALMSLPRDLRVQIPGRGTAKINEAYELGGPALTLRTVKNLTGLPINHVINV
                     DFKGFESAINAIGCIYVDVDRRYFNDSAEYAYINVPSGYQRLCGQQALEYARFRHEDT
                     DLVRGVRQQEVIRATKQQAGVKGLVEKRDELQRIFGKYTTSDASLKNSKAILGLLKLA
                     VFSAKQPIREVHFEGEVGITSGNEATGTPSFVTATDRTVKKLVQQFLGVENSKGVKGK
                     LQPEKTSGKPAKRPRRPSGDNGLEKAPGPGKDQAVQAIQQGAGGQLPVYYPTLRLKGS
                     LFTGPPRYYKIRTPGNKFHKSYRMVIKRDKLGEYYGVQGTTWKDPPLLEGGFDKVKAG
                     KHTYEVHYDGDRVKLVAWRTPKAVYWISNTLLQSLNKEQMLSIARTARLP"
     gene            complement(2060..>2439)
                     /locus_tag="VFY44_02185"
     CDS             complement(2060..>2439)
                     /locus_tag="VFY44_02185"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5909271.1"
                     /translation="LPGQNPDEILAAVNALADTEVVHVFHREPIIVARDEPHVQVLAE
                     AVSKTTPVQDSIAVGRDGTSDVISFLNAGIPGVEFGPACDGHHGPDEWVSIESLTQYR
                     KALVAFVGLVPSLGAGKPGLRVA"
BASE COUNT          315 a          818 c          880 g          426 t
ORIGIN      
        1 ccacgcggtc ggcgaagtcg tccgagccgt cgtcggcgcc gatcaccacg cggtcggggt
       61 tcatgaagtc ctggaccgcc gagccctcct tgaggaactc ggggttggag atgtagccca
      121 ggtcggcgcg gcggcgggcg atcgagcgcc cggtgcccac ggggaccgtg ctcttcatga
      181 cgatcgcgtg gtcggtcgag tcacccagct cggcgatcac ggcctccacg cgcgagaggt
      241 cggcgtcgcc ggagtacgtg ggcggcgtgt cgacgcagca gaacagcagc tcggcgttgt
      301 ccagcacctc ggccatgtcg gtggtgaagt gcagccgctc ggcgttcttg gccaggagct
      361 cgggcaggcc gggctcgtgg atggtgacct cgccgcgcga gagcgcctcc accttctcgg
      421 gcaggatgtc gcgcgcgtag acgtggtggc caagctcggc gaatgcggct gccgtgcaga
      481 ggccgaccca gcccacgccg atcacgccga tcggttcgtt gggggcgctc atgggagccg
      541 gagaggctag cggcgcaggg ctacggcagt cgcgccgtgc gcgcgatcga cagcatctgc
      601 tccttgttga ggctctggag cagggtgttg gagatccagt agacggcctt gggcgtgcgc
      661 caggccacca gcttcacgcg gtcgccgtcg tagtgcacct cgtacgtgtg cttgcccgcc
      721 ttgaccttgt cgaagccgcc ctccagcagg ggcgggtcct tccacgtggt gccctgcacg
      781 ccgtagtact cgcccagctt gtcgcgcttg atgaccatcc ggtagctctt gtggaacttg
      841 ttgccgggcg tgcggatctt gtagtagcgc ggcggcccgg tgaacagcga gcccttgagg
      901 cgcagcgtcg ggtagtacac cggcagctgg ccgcccgcgc cctgctggat ggcctgcacc
      961 gcctggtcct tgcccgggcc cggggccttc tccaggccgt tgtcgcccga cgggcgccgg
     1021 ggccgcttgg ccggcttgcc cgaggttttc tccggctgga gcttgccctt cacgcccttg
     1081 gagttctcga cgccgaggaa ctgctgcacc agcttcttga ccgtccggtc ggtggcggtc
     1141 acgaagctcg gcgtgccggt ggcctcgttg cccgaggtga tgcccacctc gccctcgaag
     1201 tgcacctcgc ggatgggctg cttggcgctg aacaccgcca gcttgagcag ccccaggatg
     1261 gccttgctgt tcttcagcga cgcgtcggag gtcgtgtact tgccgaagat ccgctggagc
     1321 tcgtcgcgct tctcgaccag gcccttgacg ccggcctgct gcttggtggc gcggatgacc
     1381 tcctgctggc gcacgccgcg caccaggtcg gtgtcctcgt ggcggaagcg cgcgtactcc
     1441 agcgcctgct ggccgcacag ccgctggtag ccggagggca cgttgatgta ggcgtactcc
     1501 gcgctgtcgt tgaagtagcg ccggtccacg tccacgtaga tgcacccgat ggcgttgatc
     1561 gcgctctcga agcccttgaa gtccacgttg atcacgtggt tgatgggcag gcccgtcagg
     1621 ttcttgaccg tgcgcagggt cagcgcgggg ccgccgagct cgtaggcctc gttgatcttg
     1681 gcggtgccgc ggccggggat ctggacgcga aggtcgcgcg ggaggctcat cagcgcggtg
     1741 gccttccggt ccgggtccag ccgggccagg atgatggtgt ccgagcgcgc ccccgtgccg
     1801 gcgcccccgt ccttcgcgtt gtcgggccgg cggtccgagc ccagcagcag gatcgtctgg
     1861 ggctcgcccg ggtcgacctc ggcgatgtcg ccggtgccca ggcggatgtc cttgcccgtc
     1921 ttcagcgtgt cgaccacgtt gtcgacctcg tggaacgcgg cgacggacgt ggcgccggcg
     1981 gtggcgaaga cgaccaggaa gcagcccagg atcagcttct tccagatccc ggggcggggc
     2041 aggtcctcga agcccgggct cacgccaccc tcagtccggg cttgccggcg ccgagcgagg
     2101 gcacgaggcc cacgaacgcc accagcgcct tgcggtactg ggtcagcgac tcgatcgaga
     2161 cccactcgtc ggggccgtgg tggccgtcac aggccggccc gaactcgacg ccggggatcc
     2221 cggcgttcag gaaggagatg acgtcggatg tcccgtcgcg gcccacggcg atgctgtcct
     2281 gcaccggggt cgtcttcgac accgcttcgg ccaggacctg cacgtgcggc tcgtcgcgcg
     2341 cgacgatgat cggctcgcgg tggaacacgt gcacgacctc ggtgtcggcc agcgcgttca
     2401 cggcggccag gatctcatcg gggttctggc ccggcaggt
//
LOCUS       DASUPW010000095         2282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1268826, whole genome shotgun sequence.
ACCESSION   DASUPW010000095 DASUPW010000000
VERSION     DASUPW010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268826"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VFY44_02190"
     CDS             <1..278
                     /locus_tag="VFY44_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909272.1"
                     /translation="DRVMRSHPNTHTHDVTVRLAHPGDAAALSDLASLDCADLPAGEL
                     MVAEAAGAIVAALPLAGGPAIADPFRRTAGLVSLLQLRRTQLGVATS"
     gene            complement(268..726)
                     /locus_tag="VFY44_02195"
     CDS             complement(268..726)
                     /locus_tag="VFY44_02195"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5909273.1"
                     /translation="MSQATLHTTHGPIEVTFFDDDAPKTVENFRKLADDGFYDGLGFH
                     RVIPDFMIQGGCPEGTGTGGPGYTFEDEFNDHKVVRGALAMANAGPNTNGSQFFIVTI
                     GEAGWLDGKHTVFGEVSSGMDAVDAIEGVPTGPGDRPSEPVFIEKVELSS"
     gene            complement(723..>2282)
                     /locus_tag="VFY44_02200"
     CDS             complement(723..>2282)
                     /locus_tag="VFY44_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5909274.1"
                     /translation="LDALEHLGYKQPTPIQEETIPLLLQGRDVIGQAQTGTGKTAAFG
                     LPMLEYVDPSANEIQALVLTPTRELCIQVTQALRAYGEQRGINVVAIFGGAPIREQAA
                     RLKDNAHVVVGTVGRVMDMIGRHHLFLDQARYVVLDEADEMLDLGFLEDVEKILGRCP
                     SGRQTALFSATMPPEIKKLAEKRMYEPETIKVKAATLTIDTVAQSYIEVSDKEKQDKL
                     TEVVKAEDPKQAIIFVRTKIGVDRLARRLGDSGMRVKTLHGDMSQGARDGVMIAFKDG
                     RERLLVATDVAARGLDIAGVSHIINYDVPNSPDVYVHRIGRTGRAGEKGRAITFITPK
                     QRSDLAAIEAHASTKIPEWGSAEDGKTGLKPDAETPAAEAPAADEPKAQEPDAPEAAA
                     EQADEKPKERRVRRGRATSNGRDPGEDQPEKREPRRRRHSKPRREDTDTEAAKIFLGA
                     GRKQGFRPADVVGAIVDHSHLDGEDVRNVRVLERFSFLEVPAARAGEVVDKVSGNEVR
                     GVKLRLEVAKR"
BASE COUNT          267 a          798 c          813 g          404 t
ORIGIN      
        1 aggatcgggt catgcgctct catccgaaca cccacacgca cgacgtcacc gtgaggctgg
       61 cccatcccgg cgatgccgcc gcgctctcgg acctggcctc gctggactgc gcggacctcc
      121 ccgcgggcga gctgatggtg gccgaggccg ccggggccat cgtcgccgcc ctgcccctcg
      181 cgggcgggcc ggcgatcgcc gaccccttcc ggcgcacggc ggggctggtg agcctgctcc
      241 agctgcgcag gacgcagctg ggggtggcta cgagctgagc tcgaccttct cgatgaacac
      301 cggctcggag ggcctgtccc ccgggccggt gggcacgccc tcgatggcgt cgaccgcgtc
      361 catgcccgac gagacctcgc cgaacacggt gtgcttgccg tccagccagc ccgcctcgcc
      421 gatggtgacg atgaagaact ggctgccgtt ggtgttcggg ccggcgttgg ccatggccag
      481 ggcgccgcgc acgaccttgt gatcgttgaa ctcgtcctcg aacgtgtagc ccgggccgcc
      541 ggtgccggtg ccctcggggc agccgccctg gatcatgaag tccgggatca cgcggtggaa
      601 gcccaggccg tcgtagaagc cgtcgtccgc gagcttgcgg aagttctcca ccgtcttggg
      661 cgcgtcgtcg tcgaagaacg tgacctcgat gggcccgtga gtcgtgtgca gcgtcgcctg
      721 gctcatcgtt tggctacctc caaccggagc ttcacgcccc ggacttcgtt tccactgacc
      781 ttgtccacca cttccccggc gcgcgccgcg ggaacctcca ggaagctgaa gcgctcgagc
      841 acgcgtacgt tgcgcacgtc ctcgccgtcc aggtgcgagt ggtccacgat cgcgcccacc
      901 acgtcggcgg ggcggaagcc ctgcttgcgg ccggccccga ggaagatctt ggccgcctcg
      961 gtgtcggtgt cctcgcggcg cggcttggag tgccggcgcc ggcgcggctc gcgcttctcg
     1021 ggctggtcct cgccgggatc gcggccgttg ctggtcgcgc ggccgcggcg gacgcggcgc
     1081 tccttgggct tctcgtcggc ctgctcggcc gcggcctccg gcgcgtcggg ctcctgggcc
     1141 ttgggctcgt cggccgcggg cgcctcggcc gcgggcgtct cggcgtcggg cttgaggccc
     1201 gtcttgccgt cctcggccga tccccactcg gggatcttgg tgctggcgtg cgcctcgatg
     1261 gcggccaggt cgctgcgctg cttgggggtg atgaacgtga tggcgcggcc cttctcgccg
     1321 gcgcggcccg tgcggccgat gcggtgcacg tacacgtcgg gggagttggg cacgtcgtag
     1381 ttgatgatgt gcgacacgcc ggcgatgtcc agcccgcgcg cggccacgtc ggtggccacc
     1441 agcaggcgct cgcggccgtc cttgaaggcg atcatcacgc cgtcacgggc gccctgggac
     1501 atgtcgccgt gcagggtctt cacccgcatg ccgctgtcgc cgaggcgccg ggccagccgg
     1561 tccacgccga tcttggtgcg cacgaagatg atggcctgct tggggtcctc ggccttgacc
     1621 acctcggtca gcttgtcctg cttctccttg tcggagacct cgatgtagga ctgcgcgacc
     1681 gtgtcgatgg tcagcgtggc cgccttgacc ttgatggtct cgggctcgta catgcgcttc
     1741 tcggccagct tcttgatctc gggcggcatc gtggcgctga acagcgccgt ctggcggccc
     1801 gaggggcagc ggcccaggat cttctcgacg tcctcgagga agcccaggtc cagcatctcg
     1861 tcggcctcgt caaggaccac gtagcgggcc tggtccagga acaggtggtg gcggccgatc
     1921 atgtccatga cccggccgac ggtgcccacg accacgtggg cgttgtcctt caggcgcgcg
     1981 gcctgctcgc ggatgggtgc gccaccgaag atggccacca cgttgatccc gcgctgctcg
     2041 ccgtaggcgc gcagggcctg ggtcacctgg atgcacagct ccctcgtggg ggtcagcacc
     2101 agggcctgga tctcgttggc cgaggggtcc acgtactcca gcatgggcag cccgaaggct
     2161 gcggtcttac cagtgcccgt ctgggcctgg ccgatcacgt cgcgcccctg caggagcagc
     2221 gggatcgtct cttcttggat aggtgtcggc tgcttgtagc cgaggtgctc gagggcgtcc
     2281 ag
//
LOCUS       DASUPW010000096         3320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_576543, whole genome shotgun sequence.
ACCESSION   DASUPW010000096 DASUPW010000000
VERSION     DASUPW010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576543"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="VFY44_02205"
     CDS             <1..1416
                     /locus_tag="VFY44_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5909275.1"
                     /translation="HADVVLVVESELVDEMAALDLRVRKAVRRNQAKLVTLTSRPGTL
                     DANGTGVRYAPGAADAALTALAAELGSPRADGSRLGSRAGAEPGTIAAVAQTLKEAGD
                     VVILWGERVAHGDRGHAAVDALLAVAAAVGVADKPESGLIEVPESANARGLREAGVLP
                     NLGPGLADAPAEGRSAARIADGLIDGDLTTLVLLHADPLVTHPGRGRWEEALAGAGNV
                     IAFADFMTEGLEEHATVVFPAEAGPEKEGTLTHPDGRLQRVRQAIGHQGETRPEWSVL
                     AELAERCGASLDVLSAPELTARLTASVPFYEGIDLESIGGTGVRWQDGDAASKLTGAE
                     LPTGQLASPPELPEGLRLGLTHSIWADPVTQHAPSLRFLAPEQRAELAPSDAKKIGVQ
                     SGDRVEVSVGDASVRATVALRQAVQPGSVFLLAGTLEDNGTALANGSPRAVDVRRIES
                     EQRSPLAVVAAGGPDAEPPPS"
     gene            1413..2429
                     /gene="nuoH"
                     /locus_tag="VFY44_02210"
     CDS             1413..2429
                     /gene="nuoH"
                     /locus_tag="VFY44_02210"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX5909276.1"
                     /translation="MTVLSATVVESTLVQIVKAIVIFAFILQVVPLVLLMERKLLGRF
                     QARYGPNRVGMFGLMQPLADVFKLLSKEAFTPATAIPWMMAIAPVIAIVSAVATLAII
                     PFGPQGAWGGDLGLYGIDVPIGVLYFFAFGSIGFYGFLLGGWASGSKYSFLGAMRAVA
                     QLISYEIAIGLSLLGVVMTAGSLSFVDIVEAQGAAEKGIWFIVPQFVGFCIFLVAGFA
                     ESSRAPFDLPESDAELVSGYNTEFGGMRFGSFFMAEYIEVIIISCVAVTCFLGGWHGP
                     GPIALAPLWMILKTFAFVVLFIWVRASLPRLRYDQLMSLGWKVLIPLGTLNILVTAIL
                     LVAT"
     gene            2440..2976
                     /gene="nuoI"
                     /locus_tag="VFY44_02215"
     CDS             2440..2976
                     /gene="nuoI"
                     /locus_tag="VFY44_02215"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEX5909277.1"
                     /translation="MTYSLPDDALVVQRGPEPGGVGGAYRAFGATLKGLRTTLARAVT
                     EKPVTVQYPEEKTPVYPRFRGKHKLHKFEETGLEKCVGCSLCAAACPAECIRVVAAEN
                     TPDNQVSAGERYAAVYEINMSRCIFCGYCEVACPFDAITMGNDYELADYERSDLIFTK
                     EMLLEDHPERTPLRREGE"
     gene            2986..>3320
                     /locus_tag="VFY44_02220"
     CDS             2986..>3320
                     /locus_tag="VFY44_02220"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX5909278.1"
                     /translation="MFFEQLLFFIAGILALGGAFGVVTLQNQFYSVLSLIAHLLGLAV
                     LFLLLRAEFLAAAQVVVYAGAVMVLYLFVVAYIGGADEPLRPESGVLRVLGPVFALVL
                     FLELAVAVLG"
BASE COUNT          410 a         1201 c         1192 g          517 t
ORIGIN      
        1 cacgccgacg tggtgctggt ggtcgagtcc gagctggtgg acgagatggc cgcgctggac
       61 ctgcgcgtgc gcaaggccgt gcgccgcaac caggccaagc tggtgacgct gaccagccgt
      121 cccggcacgc tggacgccaa cggcaccggg gtgcgctacg cgcccggcgc cgccgacgcc
      181 gccctgaccg cgctggccgc cgagctgggc tcgccgcggg ccgacggctc acggctgggc
      241 tcgcgggcgg gcgccgagcc gggcacgatc gcggccgtcg cccagaccct caaggaggcc
      301 ggcgacgtgg tcatcctctg gggcgagcgc gtggcccacg gcgaccgcgg ccacgcggcc
      361 gtggacgcgc tcctcgccgt ggccgccgcc gtcggcgtgg ccgacaagcc cgagtccggc
      421 ctgatcgagg tgcccgagtc ggccaacgcg cgcggcctgc gcgaggccgg cgtcctgccc
      481 aacctgggcc cgggcctggc cgacgccccc gccgagggcc ggtccgcggc gcggatcgcc
      541 gacggcctga tcgacggcga cctgaccacc ctggtgctgc tgcacgccga cccgctggtc
      601 acccaccccg gccgcgggcg ctgggaggag gcgctggccg gcgcgggcaa cgtgatcgcc
      661 ttcgccgact tcatgaccga gggcctggag gagcacgcca ccgtggtctt ccccgccgag
      721 gccgggccgg agaaggaggg caccctgacc catcccgacg gccggctcca gcgcgtgcgc
      781 caggccatcg gccaccaggg cgagacccgg ccggagtgga gcgtcctggc cgagctggcc
      841 gagcgctgcg gcgcgtcgct ggacgtgctc tcggcgcccg agctgaccgc gcggctgacc
      901 gcctccgtgc ccttctacga gggcatcgac ctcgagtcca tcggcggcac gggcgtgcgc
      961 tggcaggacg gcgacgccgc gtcgaagctg accggggccg agctgcccac cggccagctg
     1021 gcctccccgc cggagctgcc cgagggcctg cgcctggggc tgacccactc gatctgggcc
     1081 gacccggtga cccagcacgc gccctcgctg cgcttcctgg cgcccgagca gcgcgccgag
     1141 ctggccccct cggacgccaa gaagatcggc gtgcagtccg gcgaccgcgt ggaggtcagc
     1201 gtgggtgacg ccagcgtgcg agccaccgtg gcgctgcgcc aggcggtcca gcccggcagc
     1261 gtgttcctgc tggccggcac gctcgaggac aacggaacgg cgctggccaa cggctccccg
     1321 cgcgcggtcg acgtgcgccg gatcgagtcc gagcagcgca gccccctcgc cgtggtcgcg
     1381 gccgggggac cggacgcgga gccgccgccg tcgtgaccgt cctgtccgcc accgtcgtgg
     1441 agagcacgct ggtccagatc gtgaaggcga tcgtgatctt cgcgttcatc ctccaggtcg
     1501 tgccgctggt gctgctgatg gagcgcaagc tcctgggccg cttccaggcc cgctacgggc
     1561 ccaaccgcgt gggcatgttc ggcctgatgc agccgctggc cgacgtgttc aagctgctgt
     1621 cgaaggaggc cttcaccccc gccacggcga tcccgtggat gatggccatc gcgccggtga
     1681 tcgccatcgt ctcggccgtc gcgacgctgg cgatcatccc gttcggcccg cagggcgcgt
     1741 ggggcggcga cctcggcctg tacggcatcg acgtgccgat cggcgtcctg tacttcttcg
     1801 ccttcggctc gatcggcttc tacggcttcc tgctgggcgg ctgggcctcg ggcagcaagt
     1861 actcgttcct gggcgcgatg cgcgcggtcg cgcagctgat ctcgtacgag atcgccatcg
     1921 gcctgtcgct gctgggcgtg gtcatgaccg ccggctcgct gtccttcgtc gacatcgtcg
     1981 aggcccaggg cgcggccgag aagggcatct ggttcatcgt gccccagttc gtgggcttct
     2041 gcatcttcct ggtggccggg ttcgccgaga gctcgcgcgc cccgttcgac ctgcccgagt
     2101 ccgacgccga gctggtgtcg ggctacaaca ccgagttcgg cggcatgcgc ttcggctcgt
     2161 tcttcatggc cgagtacatc gaggtcatca tcatcagctg cgtcgcggtg acctgcttcc
     2221 tgggcggctg gcacggcccg ggcccgatcg cgctggcccc gctgtggatg atcctgaaga
     2281 ccttcgcgtt cgtcgtgctg ttcatctggg tgcgcgcctc gctgccgcgc ctgcgctacg
     2341 accagctgat gagcctgggc tggaaggtcc tgatcccgct gggaacgctg aacatcctgg
     2401 ttactgcgat tctgctcgtc gccacatgaa gggacgccca tgacctactc actcccagac
     2461 gacgcgctcg tcgtccagcg cggccccgag cccggcggcg tcggcggtgc ctaccgtgcc
     2521 ttcggcgcca cgctcaaggg cctgcgcacc acgctcgcgc gggcggtgac cgagaagccc
     2581 gtgaccgtgc agtacccgga ggagaagacc ccggtgtacc cgcgcttccg cggcaagcac
     2641 aagctgcaca agttcgagga gaccgggctg gagaagtgcg tgggctgctc gctgtgcgcc
     2701 gccgcctgcc cggccgagtg catccgcgtg gtcgcggccg agaacacgcc cgacaaccag
     2761 gtctcggccg gcgagcgcta cgccgcggtc tacgagatca acatgtcgcg ctgcatcttc
     2821 tgcggctact gcgaggtggc gtgcccgttc gacgccatca ccatgggcaa cgactacgag
     2881 ctggccgact acgaacgctc ggacctgatc ttcaccaagg agatgctcct cgaggatcac
     2941 ccggagcgca cgccgctgcg gcgcgagggg gagtagcccc gcccgatgtt cttcgagcag
     3001 ctcctgttct tcatcgccgg catcctggcg ctgggcggcg ccttcggcgt ggtcacgctg
     3061 cagaaccagt tctattccgt gctgtcgctg atcgcccacc tgctggggct ggcggtgctg
     3121 ttcctgttgc tgcgggccga gttcctggcc gccgcgcagg tggtggtcta cgccggggcg
     3181 gtgatggtgc tgtacctgtt cgtggtggcc tacatcggcg gcgcggacga gccgctgcga
     3241 cccgagagcg gcgtgctgcg ggtgctgggc ccggtgttcg ccctggtcct gttcctggag
     3301 ctggcggtgg ccgtgctggg
//
LOCUS       DASUPW010000097         4045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1962169, whole genome shotgun sequence.
ACCESSION   DASUPW010000097 DASUPW010000000
VERSION     DASUPW010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1962169"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VFY44_02225"
     CDS             complement(<1..648)
                     /locus_tag="VFY44_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5909279.1"
                     /translation="MKELAIEVHDLRKSYGPVEAVRGVSFSVDRGEVFGLLGPNGAGK
                     TTTVEILEGYRKRSGGDVEVLGHDPEQRDRDLQERVGVVLQSCGFHPQLTPREAVAHA
                     ARGYPRPRDPRETISLVGLEDKADVRIKQLSGGQQRRLDLAMALVGDPELIFLDEPTT
                     GFDPAARRVAWSAVRALKDLGKTVVLTTHYLDEAQTLADRVAIVKEGQIVAEGPPS"
     gene            692..1021
                     /locus_tag="VFY44_02230"
     CDS             692..1021
                     /locus_tag="VFY44_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909280.1"
                     /translation="MAAEAQEKGALTCSLGSSELAARHEDLAELFTAGLTSASHYGDM
                     LELRFRERYEPRVEALAAAERECCPFMEIEVNRDGREVVLVLDCPPEAAATLDPFAEL
                     AARALSG"
     gene            complement(1089..1865)
                     /locus_tag="VFY44_02235"
     CDS             complement(1089..1865)
                     /locus_tag="VFY44_02235"
                     /inference="COORDINATES: protein motif:HMM:NF006108.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX5909281.1"
                     /translation="MGIDSEQVTYERRGAAAVVTIDRQERRNAVDGPTADLLTQAYRR
                     FEEDDEARVLVLTGAGGEAFCAGADLKAIETFAPRLGLPDGPLGFTRLTPSKPTIAAI
                     GGWCLAGGLELALWCDLRLADEGSRLGFTERRFGVPLIDGGTQRLPRIIGLGRALDLI
                     LTGRVVEADEALSMGLLTEIVPAGTALERALAMAEALAQFPQETMLADRAAAIEGIGM
                     PFADGMRREAESGPMTFEVAARGAARFAGGEGRGASGAGA"
     gene            complement(2011..2259)
                     /locus_tag="VFY44_02240"
     CDS             complement(2011..2259)
                     /locus_tag="VFY44_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909282.1"
                     /translation="MKARALPLLTLIFTLALAVPALAHDNGEGLVGETNDRIITFFSL
                     GVVIFFFLVVCFGSWVQGRLEKRKDARKAADLKSSIGW"
     gene            2294..2788
                     /locus_tag="VFY44_02245"
     CDS             2294..2788
                     /locus_tag="VFY44_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX5909283.1"
                     /translation="MSDAAFSCIIEIPKGSRNKYEWDHERERLVLDRFLFSSVVYPTD
                     YGFIPDTLSQDGDPLDVMCCVSEATFPGCMIDVKPIALFRMEDDQGIDAKVLCVPLSD
                     PGWNTMETLEDLPELLTNEITHFFSIYKDLEQKKVTVDGWYSREDAIAEIEESRARFR
                     DQQS"
     gene            complement(2785..3069)
                     /locus_tag="VFY44_02250"
     CDS             complement(2785..3069)
                     /locus_tag="VFY44_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX5909284.1"
                     /translation="MIGFLVAGLIIGLLARLLLPGRQKIGLLWTLGLGVVGSLIGGTI
                     ANFIGSGDIWELNIIGFVCAVVASVAVLSVAESAGLGRGKKTDQLDKPRY"
     gene            3094..3450
                     /locus_tag="VFY44_02255"
     CDS             3094..3450
                     /locus_tag="VFY44_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909285.1"
                     /translation="MKLAGQIGAIALVALAITLLPGGGTAVEVILTALTLTFLVAIAF
                     FGFRLFNQFRFELESLEDRDRAVLYGSFAVAIFAFAAAQRMFDTGGGGIIAWFALIGI
                     AVYGLYWVWTRYRRLA"
     gene            3531..3881
                     /locus_tag="VFY44_02260"
     CDS             3531..3881
                     /locus_tag="VFY44_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909286.1"
                     /translation="MSRREASEHGQASIELLAALPFVLLAGAVAWQLALAGWTAWMSA
                     HAARVAARADAVGRPPGPAARSALPAPLRPGLRVRRRASGGVEVRVRVPALPAGVHGP
                     VAITARSSLGRGER"
BASE COUNT          533 a         1474 c         1434 g          604 t
ORIGIN      
        1 cgagggcggc ccctcggcca cgatctggcc ctccttgacg atggccacgc ggtcggccag
       61 cgtctgggcc tcgtccaggt agtgggtggt cagcaccacg gtcttgccca ggtccttgag
      121 cgcccggacg gcgctccagg ccacccgccg ggcggcgggg tcgaagccgg tggtcggctc
      181 gtccaggaag atcagctcgg ggtcgcccac cagcgccatc gccaggtcca gccgccgctg
      241 ctggccgccg gagagctgtt tgatgcgcac gtcggccttg tcctccaggc ccaccagcga
      301 gatcgtctcg cgcgggtcgc gcggcctggg gtagccgcgg gcggcgtggg cgacggcctc
      361 gcgcggggtc agctgggggt ggaagccgca gctctggagc accacgccga cgcgctcctg
      421 gaggtcacgg tcgcgttgct cgggatcgtg ccccagcacc tcgacgtcgc caccggagcg
      481 cttgcggtag ccctccagga tctcgaccgt ggtggtcttg ccggccccgt tggggccgag
      541 caggccgaag acctcgccgc ggtccacgga gaacgagacg ccccggaccg cctcgaccgg
      601 cccgtagctc ttgcggaggt cgtggacctc gatcgcgagc tctttcacgc agatgactat
      661 gccaactccg gatcgtgggt atcctggaga cgtggctgcg gaggcacagg agaagggggc
      721 gctcacgtgc tccctgggct cgtccgagct ggctgcgcgc cacgaggacc tcgccgagtt
      781 gttcaccgcc ggcctgacgt cggccagcca ctacggcgac atgctcgagc tgcgcttccg
      841 tgagcgctac gagccgcggg tcgaggcgct ggccgccgcc gagcgcgagt gctgcccgtt
      901 catggagatc gaggtcaacc gggacgggcg cgaggtcgtg ctcgtcctgg actgcccgcc
      961 cgaggcggcc gcgacgctgg atcccttcgc cgagctcgcc gctcgggccc tttccgggta
     1021 gcccttgcgg ccatccatgg ccgcgccttg acctggccgg cgccgtgacc ggccggctgt
     1081 gccggcctct aggccccggc tccgctcgcc ccgcggccct cgccgcctgc gaaccgcgcc
     1141 gcgccgcgcg ccgcgacctc gaacgtcatc ggcccggatt cggcctcacg ccgcatcccg
     1201 tcggcgaacg gcatgccgat gccctcgatc gcggcggcgc ggtcggccag catcgtctcc
     1261 tgcgggaact gggccagcgc ctcggccatc gccagcgcgc gctccagcgc ggtgccggcg
     1321 ggcacgatct cggtcagcag gcccatcgac agggcctcgt cggcctcgac cacgcgcccg
     1381 gtcaggatca ggtcgagcgc ccggcccagc ccgatgatcc gcggcagccg ctgggtgccg
     1441 ccgtcgatca gcggcacgcc gaaccggcgc tcggtgaagc ccaggcgcga gccctcgtcg
     1501 gccaggcgca ggtcgcacca cagcgccagc tccaggccgc cggccaggca ccagccgccg
     1561 atcgcggcga tggtgggctt cgatggcgtc agccgggtga accccagcgg cccgtcgggc
     1621 agccccagcc ggggcgcgaa cgtctcgatc gccttcaggt cggcgcccgc gcagaaggcc
     1681 tcgccacccg cgcccgtcag caccagaacg cgcgcctcgt cgtcctcctc gaaccggcgg
     1741 taggcctggg tcagcaggtc ggcggtcggg ccgtcgaccg cgttgcggcg ctcctggcgg
     1801 tcgatcgtca ccacggccgc cgccccgcgc cgctcgtagg tcacctgctc ggagtcgatc
     1861 cccatcccgc cgcgatcctc tcagaaaaca cgaacggccc cggagggaac cggggccgcg
     1921 cgctgggagg gatctgcacc gcccatgctg gcgggcgtcc gggcctccgt tgtgacgttt
     1981 ctgagaatcc gctctgcgga ttgcccgcaa ctaccagccg atagacgact tgaggtccgc
     2041 ggccttgcgg gcgtccttgc gcttctcgag ccggccctgg acccaggagc cgaagcacac
     2101 gaccaggaag aagaagatca cgacgccgag cgagaagaag gtgatgatcc ggtcgttcgt
     2161 ctcgccgacg aggccctcgc cgttgtcgtg ggccagggcc ggaacggcca gggccagcgt
     2221 gaagatcagg gtcaggaggg ggagtgctcg cgccttcatc ggcgccgatc ctaccgggta
     2281 gtctcaccgg accgtgagcg acgccgcctt cagctgcatc atcgagattc ccaaggggag
     2341 ccggaacaag tacgagtggg accacgagcg cgagcgcctg gtcctggacc ggttcctgtt
     2401 ctcctcggtg gtgtatccca ccgactacgg gttcatcccc gacacgctca gccaggacgg
     2461 cgacccgctg gacgtcatgt gctgcgtctc ggaggccacg ttcccgggct gcatgatcga
     2521 cgtgaagccg atcgcgctgt tccggatgga ggacgaccag ggcatcgacg ccaaggtcct
     2581 gtgcgtgccg ctctcggacc cgggctggaa cacgatggag acgctcgagg acctgcccga
     2641 gctgctgacc aacgagatca cccacttctt ctcgatctac aaggacctcg agcagaagaa
     2701 ggtcacggtc gacggctggt actcacgcga ggacgcgatc gccgagatcg aggagtcccg
     2761 cgcgcgcttt cgcgaccagc agtcctagta gcgcggcttg tccagctggt cggtcttctt
     2821 gccccggccc aggccggcgc tctcggccac gctgagcacg gccaccgagg cgaccacggc
     2881 gcagacgaag ccgatgatgt tcagctccca gatgtcgccg gagccgatga agttggcgat
     2941 cgtccccccg atcagcgacc cgacgacgcc cagccccagc gtccaaagca ggccgatctt
     3001 ctggcggccc ggtaggagca gccgggcgag gaggccgatg atgaggccgg ctacgaggaa
     3061 tccgatcatg ccccgcacgt tagcctgcgc agggtgaagc tggccggcca gatcggggcg
     3121 atcgccctcg tcgctctcgc catcacgctg ctccccgggg gagggacggc ggtcgaggtg
     3181 atcctcaccg ccctgacgct gaccttcctg gtcgcgatcg ccttcttcgg cttccggctg
     3241 ttcaaccagt tccggttcga gctcgagtcg ctggaggacc gggaccgcgc ggtgctctac
     3301 ggctcgttcg cggtggccat cttcgccttc gccgccgccc agcggatgtt cgacaccggt
     3361 ggcggcggga tcatcgcctg gttcgcgctc atcggcatcg ccgtgtacgg cctgtactgg
     3421 gtgtggaccc ggtaccgccg gctggcctga tcgcgctttc tcacacgccc gtcgcacccg
     3481 tgtaacccgc gcgcgttgtc ccgtgaccgg caccgcggga ccgtcttccc atgtcccgcc
     3541 gggaggcatc cgagcacggc caggccagca tcgagctgct ggccgcgctg ccgttcgtgc
     3601 tcctggcggg cgcggtggcc tggcagctcg cgctggcggg ctggaccgca tggatgtccg
     3661 cgcacgccgc ccgggtggcc gcgcgcgccg acgccgtcgg ccgcccgccc gggcctgcgg
     3721 cgcgtagcgc gctgcccgcg ccgctgcggc ccgggttgcg ggtccgtcgg cgcgcctccg
     3781 gcggcgtcga ggtccgcgtc cgggtcccgg cgctgccggc aggtgtgcac gggccggtcg
     3841 ccatcacggc ccggtcgtcg ctgggccggg gggagcggtg agccggcagg ggtcgcagcg
     3901 gggccaggcg ctcgtcgagg tgctcggtgc cgtgccggtg atgctgctgg tgggactcgt
     3961 cttgctgcag gcgctcgccg tcggatatgc ggcggtgctc gccgggaccg ccgcccacgc
     4021 cggagccctg gcgttggctg ccggc
//
LOCUS       DASUPW010000098         2823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_439150, whole genome shotgun sequence.
ACCESSION   DASUPW010000098 DASUPW010000000
VERSION     DASUPW010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439150"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VFY44_02265"
     CDS             complement(<1..326)
                     /locus_tag="VFY44_02265"
                     /inference="COORDINATES: protein motif:HMM:NF013490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX5909287.1"
                     /translation="MSHDHATVAEEEYLQVIFWLQEAGLPMTGANVARAMQVSAPTVH
                     EMIGRLEADGYVTRAGDKSLSFTETGLEHAEGIVRRHRLIERFLTDVFDIPWDQVHEE
                     AEKLEH"
     gene            379..1338
                     /locus_tag="VFY44_02270"
     CDS             379..1338
                     /locus_tag="VFY44_02270"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX5909288.1"
                     /translation="MPLPLEPPIKPQLAKSAKKLPEGDQWRYEPKWDGFRTLAFKDGD
                     EVYLQSRGGKPMNRYFPDVVEQVAAVPEERVVLDGEMVVEVDGVQEFDMLSQRIHPAE
                     SRVKMLAEQTPARYVAFDVLAVGDEVLMELGYDERRERLAKLKLGPVEESPSVRTPDE
                     AGQWLTGNSEGVVAKEGGAAYLPGQRKGMVKIKRLRTLDAVVAAFRYGKEEGTLGSLI
                     LGLYDDDGQLHVVGHTSGFKAKEKRELLDKLAPYMTGESGPGEPSRWKSDEDLVWEGM
                     RPELVVEVSYDHVTGNRIRHGTRLLRWREDKAPQECKLEQLEA"
     gene            1338..2399
                     /gene="ligD"
                     /locus_tag="VFY44_02275"
     CDS             1338..2399
                     /gene="ligD"
                     /locus_tag="VFY44_02275"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX5909289.1"
                     /translation="MAKKGDATVEVDGREVRVSSADRVIFPATGRTPEVTKLGVVEYY
                     VAVGEGIMRALRERPTTLERWPKGVHEGFTLSTREGHKGEAFYQKRVPKGAPDYLETA
                     RIEFPSGRSADEICPTELAVVAWCGHMGTITFHPWPVRRADVDHPDEMRIDLDPQEGT
                     DFADAQVVAAEARKLLEELGYRGFPKTSGGRGIHIYVRIEPRWTFTDVRHAVIAFGRE
                     LSRRMPEQVTTKWWKEERGERVFIDYNQNARDRTIASPYSIRAKPGAPVSAPLLWEEV
                     AEIKPEDFTVDTMPARFAEVGDRFAEIDDVHHSLEPLLEMYQRDEEGDMPYPPDYPKM
                     PGEPKRVQPSRDTDRKKES"
     gene            2402..>2823
                     /locus_tag="VFY44_02280"
     CDS             2402..>2823
                     /locus_tag="VFY44_02280"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEX5909290.1"
                     /translation="MQLEVLQTDVTKLEVDAIANAANTDLLHGGGVAAAIERAGGARV
                     REESLAAAPIGLGEAVETTAGDMPSRWVIHAATMELGGPSSAEIIERATVSTLQVADR
                     LGARSLGLVAFGTGVGGFPAGEAARLMVGAVRAHAAGSL"
BASE COUNT          447 a          953 c         1061 g          362 t
ORIGIN      
        1 cagtgctcga gcttctcggc ctcctcgtgc acctggtccc acgggatgtc gaagacgtcg
       61 gtcaggaagc gctcgatcag gcggtggcgg cggacgatgc cctcggcgtg ctcgagcccc
      121 gtctcggtga acgagagcga cttgtcgccg gcacgggtca cgtagccgtc ggcctcgagc
      181 cggccgatca tctcgtgcac ggtgggagcg gacacctgca tcgcccgggc cacgttggcg
      241 ccggtcatgg gcaggccggc ctcctggagc cagaagatga cttggaggta ctcctcctcg
      301 gcgacggtgg cgtgatcgtg actcatggcc tgaactgtat tccctccggg tgccgcggcg
      361 aatgggatca tgccccgcat gcccctcccg ctcgagcccc cgatcaagcc ccagctggcc
      421 aagtccgcga agaagctccc ggagggcgac cagtggcgct acgagcccaa gtgggacggc
      481 ttccgcacgc tggccttcaa ggacggcgac gaggtctacc tgcagagccg gggcggcaag
      541 ccgatgaacc ggtacttccc cgacgtggtc gagcaggtgg ccgccgtgcc cgaggagcgg
      601 gtggtcctgg acggcgagat ggtggtcgag gtcgacggcg tgcaggagtt cgacatgctc
      661 agccagcgca tccaccccgc cgagtcgcgg gtgaagatgc tggccgagca gacgccggcc
      721 cggtacgtgg cgttcgacgt gctggccgtg ggcgacgagg tgctcatgga gctgggctac
      781 gacgagcgcc gcgagcggct ggcgaagctg aagctgggcc cggtcgagga gtcgcccagc
      841 gtgcgcacgc ccgacgaggc cggccagtgg ctgaccggca actccgaggg cgtcgtggcc
      901 aaggagggcg gggccgccta cctgcccggc cagcgcaagg gcatggtcaa gatcaagcgg
      961 ctgcgcaccc tggacgccgt ggtggccgcc ttccgctacg gcaaggagga gggcacgctg
     1021 ggctccctga tcctcggcct ctacgacgac gacggccaac tgcacgtggt cgggcacacg
     1081 tcgggcttca aggccaagga gaagcgcgag ctgctggaca agctggcgcc ctacatgacc
     1141 ggcgagagcg ggcccgggga gcccagccgc tggaagtcgg acgaggacct ggtgtgggag
     1201 ggcatgcgcc cggagctcgt ggtcgaggtc agctacgacc acgtgaccgg caaccgcatc
     1261 cgccacggaa cccggctgct gcgctggcgc gaggacaagg cgccgcagga gtgcaagctg
     1321 gagcaactcg aggcctgatg gccaagaagg gcgacgccac cgtggaggtc gacggccgcg
     1381 aggtgcgtgt ctccagcgcc gaccgcgtga tcttcccggc caccggccga acgccggagg
     1441 tcaccaagct gggcgtggtc gagtactacg tcgccgtcgg cgaggggatc atgcgcgcgc
     1501 tgcgtgagcg gccgaccacc ctggagcgct ggcccaaggg cgtgcacgag ggcttcacgc
     1561 tctcgacccg cgagggccac aagggcgagg ccttctacca gaagcgggtg cccaagggtg
     1621 cccctgacta cctggagacg gcccggatcg agttcccctc gggccgcagc gccgacgaga
     1681 tctgccccac cgagctcgcg gtggtggcgt ggtgcgggca catgggcacg atcaccttcc
     1741 acccctggcc cgtgcgccgg gccgacgtgg accaccccga cgagatgcgg atcgacctgg
     1801 acccccagga gggcaccgac ttcgcggacg cccaggtggt ggccgccgag gcccggaagc
     1861 tgctggagga gctgggctac cggggcttcc cgaagacgag cggcgggcgc ggcatccaca
     1921 tctacgtgcg catcgagccc aggtggacgt tcaccgacgt gcgccacgcg gtgatcgcgt
     1981 tcgggcgcga gctgtcgcgg cggatgcccg agcaggtgac caccaagtgg tggaaggagg
     2041 agcgcgggga gcgggtgttc atcgactaca accagaacgc gcgcgaccgg accatcgcct
     2101 cgccctactc gatccgcgcc aagccgggcg cccccgtctc ggccccgctg ctgtgggagg
     2161 aggtggccga gatcaagccc gaggacttca ccgtggacac gatgccggcc cgcttcgccg
     2221 aggtcggcga ccgcttcgcc gagatcgacg acgtccacca ctcgctggag ccgctgctgg
     2281 agatgtacca gcgcgacgag gagggcgaca tgccctaccc gcccgactac cccaagatgc
     2341 cgggcgagcc caagcgggtg cagccctcgc gggacaccga ccgcaagaag gagtcctgag
     2401 cgtgcagctc gaggtgctcc agacggacgt cacgaagctc gaggtcgacg cgatcgccaa
     2461 cgcggcgaac accgacctgc tgcacggcgg cggcgtggcc gcggcgatcg agcgcgccgg
     2521 cggcgccagg gtccgcgagg agtcgctggc cgccgcgccg atcgggctgg gcgaggccgt
     2581 ggagaccacc gccggcgaca tgccctcgcg gtgggtcatc cacgcggcca cgatggagct
     2641 gggcgggccg tcgtcggcgg agatcatcga gcgcgcgacg gtgtccaccc tgcaggtggc
     2701 cgaccggctc ggagcgcggt cgctggggct ggtggccttc ggcaccggcg tgggcggctt
     2761 ccccgccggc gaggcggcgc ggctgatggt gggcgcggtg cgcgcgcacg cggcgggctc
     2821 gct
//
LOCUS       DASUPW010000099         3223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_300608, whole genome shotgun sequence.
ACCESSION   DASUPW010000099 DASUPW010000000
VERSION     DASUPW010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300608"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VFY44_02285"
     CDS             complement(<1..527)
                     /locus_tag="VFY44_02285"
                     /inference="COORDINATES: protein motif:HMM:NF023425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ice-binding family protein"
                     /protein_id="HEX5909291.1"
                     /translation="MSNRTRSRGSRRCGVALAVGLASLAAPSFVQAAEVEPALGTAYD
                     YSVLAGQGVTNVGPTFLQLDLGSSPNGAFADNGVLTQTPGAQQHFADAQALQAKSDLG
                     TGYDTAAAEPSSATKTGQDLVGQTLTPGTYTWAGSITHSGGAVTLDGQGDPAARFIFQ
                     VAADMATQTGFSVNL"
     regulatory      complement(614..690)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            874..1680
                     /locus_tag="VFY44_02290"
     CDS             874..1680
                     /locus_tag="VFY44_02290"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5909292.1"
                     /translation="MSTPAGPDTILLVHGFWVTPRSWEKWIAHYEAKGYTVIAPAYPG
                     FEVEVESLNEDDTPIVDVRVPDIVENFENAIKDLDRPPIIIGHSAGGAFTQVLLDHGF
                     GAAGVALNSAPTEGVRIVPPSQVKSTWPVLKNPANHHKAVGFTFEQWNYAFTNGLPEE
                     LAKETYERYAIPASGRILFDSVTANFIPGPQATAVDYKNPDRAPLLFVSGTEDHIMPP
                     SIQQSNHKHHKGEGTITEITEYEGPHLMIAAEGWEKIADDVLEWAVEHAR"
     gene            1677..2459
                     /locus_tag="VFY44_02295"
     CDS             1677..2459
                     /locus_tag="VFY44_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5909293.1"
                     /translation="MTQVTVTHIGGPTVLLEIEGWRLLTDPTFDPPGGHYKFGWGTAS
                     DKLAGPALAPAELGRIDAVLLSHDHHEDNLDAAGRELLGSVGTVVTTVAGAGRLGGGA
                     VGLGEYETTVLEQEGRPPITVTGTPCRHGPPLSRPIVGQVTGFALAWEGQRDGPRWIS
                     GDTVLYDGVKTVADRIDVGTAILHLGGVRFPVSGPLRYTLTGAEAVELCGLLEPATVI
                     PVHYEGWKHFREGRAGAESAFAGADGPAPTWLEIGTPATIEV"
     gene            2589..2942
                     /locus_tag="VFY44_02300"
     CDS             2589..2942
                     /locus_tag="VFY44_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX5909294.1"
                     /translation="MKLVIGIVRPEKANDVLAALYRAEMRGVTMSRVQGHGGEFDRVE
                     TYRGTTVQMGLSDKVRFEVAVSDDFVDPAIDALCEGARTGEVGDGKIFVLPLERAVRI
                     RTGEADQGALTPVGA"
     gene            2942..>3223
                     /locus_tag="VFY44_02305"
     CDS             2942..>3223
                     /locus_tag="VFY44_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909295.1"
                     /translation="MDTALTLVAAKIDSGDTAWMLMATALVVMMTPALGLFYAGLVRS
                     KNTLNTFMMCVAALAVATVTWAAVGYSFAFSDGNGFIGGFGFAFLHGVDF"
BASE COUNT          486 a         1089 c         1167 g          481 t
ORIGIN      
        1 atcaggttca cgctgaagcc ggtctgcgtt gccatgtcgg cggcaacttg gaagatgaat
       61 cgtgccgcgg ggtcgccctg accgtcgagg gtgaccgcgc cgccgctgtg cgtgatgctg
      121 ccggcccagg tgtacgtgcc cggcgtcagg gtctgtccga ccagatcctg gcccgtcttg
      181 gtcgcggagc tcggctctgc tgcagcggtg tcgtacccgg tgcccagatc cgacttggcc
      241 tgcaatgcct gtgcgtcggc gaagtgctgc tgtgcccctg gcgtctgggt gagcacgccg
      301 ttgtcggcga acgcgccgtt gggcgagcta ccgagatcga gctggaggaa cgtcggaccg
      361 acgttggtca cgccctggcc ggccaggacc gagtagtcgt acgcggtgcc gagcgcgggt
      421 tcgacctcgg cggcctgtac gaaggagggg gcggcgagcg acgcgagccc tacggcgagg
      481 gcgacgccgc agcgtcgtga tcccctggaa cgggtgcggt tggacatggg agtccttccc
      541 tatgggcgag cagcccgtgc aacgttcctt gacagccact gggcgtccgg ggaagccccg
      601 gtgtggtgtc tcctctggta gcccggctga ctccggtgga gtcccgtggc tttgcgagcc
      661 cgcctcgcgg cgagtgtgcc cgtgtcgaga gaactgctct tggtgtgcgc attgcatcga
      721 cagcgcgggt cgcttccttg aggcgcctac gtcattcgcc tagtgcggat cggcttgcgc
      781 cggcgcgcgt ggtcggagat gctcgtgcca accggcacgt cgggatccgg ttttgaccac
      841 aggatcagcc accaaccagg agccagaacc accatgagca cccccgccgg tcccgacacg
      901 atcctcctcg tccacggttt ctgggtcacg cccaggagct gggagaagtg gatcgcccac
      961 tacgaggcga aggggtacac ggtcatcgcg ccggcctacc ccggcttcga ggtcgaggtc
     1021 gagtccctga acgaggacga cacgccgatc gtcgacgtgc gcgtcccgga catcgtcgag
     1081 aacttcgaga acgcgatcaa ggacctggac cggccgccga tcatcatcgg ccactcggcc
     1141 ggcggcgcct tcacccaggt gctgctcgac catggcttcg gcgcggccgg cgtggcgctg
     1201 aactccgcgc ccaccgaggg tgtccggatc gtgccgccgt cgcaggtcaa gtccacctgg
     1261 ccggtgctca aaaaccccgc caaccaccac aaggcggtcg gcttcacctt cgagcagtgg
     1321 aactacgcgt tcaccaacgg gcttccggag gagctggcga aggagaccta cgagcggtac
     1381 gccatcccgg cctcgggccg gatcctgttc gacagcgtca cggccaactt catccccggc
     1441 ccgcaggcca ccgcggtgga ctacaagaac cccgaccgcg cgccgctgct gttcgtctcc
     1501 ggcaccgagg accacatcat gccgccgtcg atccagcagt ccaaccacaa gcaccacaag
     1561 ggcgagggca cgatcaccga gatcaccgag tacgagggcc cgcacctgat gatcgcggcc
     1621 gagggctggg agaagatcgc cgacgacgtg ctggagtggg cggtcgagca cgcccgatga
     1681 cccaggtcac ggtcacccac atcggcgggc cgacggtcct gttggagatc gagggctggc
     1741 ggctgctgac cgaccccacc ttcgacccgc cggggggtca ttacaagttc ggctggggca
     1801 ccgcgtcgga caagctggcc gggccggcgc tggcgcccgc cgagctgggc cggatcgacg
     1861 cggtgctgct cagccacgac caccacgagg acaacctgga cgccgccggg cgcgagctgc
     1921 tggggtccgt gggcaccgtg gtgaccacgg tggcgggggc gggccgcctg ggcggcggcg
     1981 ccgtgggcct cggcgagtac gagacgaccg tgctggagca ggagggcagg ccgccgatca
     2041 ccgtgaccgg cacgccgtgc cgccacggcc cgccgctgag caggccgatc gtgggccagg
     2101 tgaccggctt cgcgctggcg tgggagggcc agcgcgacgg cccgcggtgg atctcgggcg
     2161 acaccgtgct ctacgacggg gtgaagaccg tggccgaccg gatcgacgtg ggcacggcga
     2221 tcctgcacct gggcggcgtg cggttccccg tctcggggcc gctgcgctac accctgaccg
     2281 gcgccgaggc ggtcgagctg tgcggcctcc tggagccggc gacggtgatc cccgtgcact
     2341 acgagggctg gaagcacttc cgcgaggggc gcgccggggc cgagagcgcg ttcgccgggg
     2401 cggacgggcc cgcgccgacg tggctggaga tcgggacgcc ggccacgatc gaggtctgac
     2461 cggccgcttc gccacctgga caaaccgccg cgcccgattt ctggacaagg ggccaatggc
     2521 atccgggcgg gcgccggaaa agctcccgcg gtcgcgtccc ccgccgacca atccaggagg
     2581 ccccccaagt gaagctggtg atcgggatcg tccggccaga gaaggccaac gacgtcctcg
     2641 cagccctgta ccgcgccgag atgcgcggtg tgaccatgag ccgtgtgcag ggccacggcg
     2701 gtgagttcga ccgcgtggag acctaccgcg ggaccacggt ccagatgggg ctgtcggaca
     2761 aggtccggtt cgaggtcgcg gtgtccgacg acttcgtgga ccccgccatc gacgccctgt
     2821 gcgagggggc gcgcaccggt gaggtgggcg acggcaagat cttcgtgctg ccgctggagc
     2881 gcgccgtccg gatccgcacc ggcgaggccg accaaggcgc gctgaccccg gtgggcgcgt
     2941 gatggacacg gcgctgacgc tggtcgcggc gaagatcgac agcggtgaca ccgcgtggat
     3001 gctcatggcc accgcgctcg tggtcatgat gacgccggcc ctcggcctgt tctacgcggg
     3061 cctggtgcgc tccaagaaca ccctgaacac gttcatgatg tgcgtcgcgg cgctggcggt
     3121 ggccaccgtg acgtgggccg ccgtggggta ctcgttcgcg ttctcggacg gcaacggctt
     3181 catcggcggg ttcggcttcg cgttcctgca cggcgtcgac ttc
//
LOCUS       DASUPW010000100         3322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1269442, whole genome shotgun sequence.
ACCESSION   DASUPW010000100 DASUPW010000000
VERSION     DASUPW010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269442"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1779)
                     /locus_tag="VFY44_02310"
     CDS             complement(<1..1779)
                     /locus_tag="VFY44_02310"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5909296.1"
                     /translation="MSSLLASVARSLTAHWKRGLIGLVVVIALVGVAVGSQSGKAAQD
                     FAIPGTESQKAQDLLETKFPAAAGAQSQVVYSVKDGRIGAPQREAIAGVNEKIADLPH
                     VTGSAAQLDRGLRVSEDGQTALSTIAYDRQSTELEPEDGKELEKVARTAEGDGLSVAL
                     RGESVDLASQQEAPVGELIGVGIAIILLTVLFRSLAAMFVTLIGALVGVFISQLILAA
                     VSKPIGIPDFATTIAVMLGLGAGIDYALVIFSRFREQLAAGDSPPEAAARANATSGTS
                     VVAAGLIVMVAIAGLLAVGIPLVGKMGVGSAIAVAMVVISSITVLPIFAGALARWLKP
                     KDPKHVAASPGFASWGRRLTRRPWVPVIAGGVVLLMLAIPVTDLRLGQPDDGNQPAEK
                     TQRVAYDQLSKGFGPGSNGPLLLAVGSKEGGELDQSELTGLTRELKGTPGVAQVTPPQ
                     PNKAGDAAIVSVIPETSPQDKATSALVDRLRDRTIPAATRDGDLNVYVGGLTASFEDF
                     SDKIASRLPLFIGIVVGLSILLLIAVFRSIWVPLASAVFNLLSIGAAYGVVVAVFQWG
                     WGASLLGAAEGTPIVSFIPLMMFAILF"
     gene            1857..2474
                     /locus_tag="VFY44_02315"
     CDS             1857..2474
                     /locus_tag="VFY44_02315"
                     /inference="COORDINATES: protein motif:HMM:NF037625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX5909297.1"
                     /translation="MAIDEVGLRERKKERTRQTLAEAAAELFDLQGFQDTTIRQIADA
                     AEVSPRTVSAYFPVKEELVFCDHREMFDQLEQRLEERPDGATTADTLRAWLMELLESV
                     DVDLEQSRRTRELIESDPALRIYERGLQERAEQMVAKAVAVDLGVSADELVPHMVGAA
                     TIAALDAMGRRAKDPGAADFQATALTVVDDAMAFIGGGVQALADR"
     gene            complement(2471..>3322)
                     /locus_tag="VFY44_02320"
     CDS             complement(2471..>3322)
                     /locus_tag="VFY44_02320"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX5909298.1"
                     /translation="EADRAAEAARAGADCTVIDAEGHYEGKYVSAQTYMARLRRKVGP
                     DYEVALTSFPYVHYHPGFPYSVFMGPGGAQANLPQMYWKTIGTSVDTIYRNTWTYNKV
                     YGRPIYPLGQTYSNPKAADVRRFRLLAGTYGATGLSWWVWQFSGARQWTALGSPFSPF
                     GTPVRRDTYPLLKRGYRSDLVVWLQQHLMKAGLLRTVNGNYGSGTETAVRAFQTSRGL
                     VASGRMDPPTWQALLPVGPAAVKWGPKRTAIQVIAAGKRRVSVQPVPLNASREARHNE
                     LAGAPGR"
BASE COUNT          497 a         1147 c         1219 g          459 t
ORIGIN      
        1 gaacaggatc gcgaacatca tcagggggat gaacgagacg atcggcgtgc cctcggcggc
       61 gcccaggagc gacgcgcccc agccccactg gaacaccgcg accaccacgc cgtaggcagc
      121 gccgatggac agcaggttga acaccgccga ggccagcggc acccagatcg agcggaagac
      181 cgcgatcagc agcaggatcg agaggccgac cacgatgccg atgaacagcg gcagccggct
      241 ggcgatcttg tccgagaagt cctcgaacga ggcggtcagg ccgcccacgt acacgttcag
      301 gtcgccgtcc cgtgtcgccg ccgggatggt ccggtcgcgc agccggtcca ccagcgccga
      361 cgtcgccttg tcctgcggcg aggtctcggg gatcaccgag acgatggccg catcgcccgc
      421 cttgttgggc tggggcggag tgacctgggc cacgcccggg gtgcccttga gctcgcgcgt
      481 caggccggtc agctcggact ggtcgagctc gccgccttcc ttgctgccca cggccaggag
      541 cagcgggccg ttcgagccgg gcccgaagcc cttgctgagc tggtcgtagg ccacccgctg
      601 ggtcttctcg gccggctggt tgccgtcgtc gggctggccc agacggaggt cggtcacggg
      661 gatggccagc atcagcagca cgacgccgcc cgcgatcacg gggacccatg ggcgccgtgt
      721 caggcggcgg ccccagctgg cgaacccggg ggaggcggcc acgtgcttgg ggtccttcgg
      781 cttgagccag cgggccagcg cgccggcgaa gatcggcagg acggtgatcg acgagatcac
      841 gaccatggcc acggcgatgg ccgagcccac gcccatcttg cccaccagcg ggatgcccac
      901 ggccagcagt ccggcgatgg cgaccatcac gatcaggccc gctgcgacca cggaggtgcc
      961 cgacgtggcg ttggcccgcg ccgccgcctc gggtgggctg tccccggccg cgagctgctc
     1021 gcggaagcgg ctgaagatga ccagcgcgta gtcgatgccc gcacccaggc ccagcatcac
     1081 cgcgatggtg gtggcgaagt cggggatgcc gatcggcttg gagaccgcgg ccaggatcag
     1141 ctggctgatg aacacgccga cgagcgcgcc gatcagggtc acgaacatcg cggccagcga
     1201 gcggaacagg accgtcagca ggatgatcgc gatgcccacg ccgatcagct cgcccaccgg
     1261 ggcctcctgc tgggaggcca ggtcgacgct ctcgccgcgc aacgcgaccg agaggccgtc
     1321 gccctcggcg gtgcgggcga ccttctccag ctccttgccg tcctcgggct cgagctccgt
     1381 ggactggcgg tcgtaggcga tggtcgagag cgcggtctgc ccgtcctcgg acacgcgcag
     1441 gccgcggtcc agctgggccg cggagccggt cacgtgcggc aggtcggcga tcttctcgtt
     1501 caccccggcg atggcctcgc gctggggggc gccgatgcgc ccgtccttga cgctgtacac
     1561 cacctggctc tgcgcgccgg cggccgccgg gaacttggtc tcgagcaggt cctgggcctt
     1621 ctgcgactcg gtgcccggga tcgcgaagtc ctgggccgcc ttgccgctct gcgagcccac
     1681 ggccacgccg accagggcga tcacgaccac gaggccgatc aggccgcgct tccagtgagc
     1741 tgtgagcgag cgggctacgg aggccaggag ggaagacatt cctcgaacgt actacgactg
     1801 caattttgcg tcaactgtaa gtttgcgcac actgcaacta tgtgtatggt tgggccatgg
     1861 cgatcgacga ggtgggcctg agggagcgca agaaggagcg cacgcgccag acgctggcgg
     1921 aggcggcagc ggagctgttc gacctccagg gcttccagga cacgacgatc aggcagatcg
     1981 ccgacgccgc cgaggtgtcg ccgcggacgg tgtccgccta cttcccggtg aaggaggagc
     2041 tggtcttctg cgaccaccgg gagatgttcg accagctcga gcagcggctg gaggagcgcc
     2101 cggacggggc caccaccgcc gacacgctgc gggcatggct gatggagctg ctcgagagcg
     2161 tggacgtgga cctcgagcag tcgcgccgca cgcgcgagct gatcgagtcc gatcccgctc
     2221 tgcgcatcta cgagcggggg ctccaggaac gggccgagca gatggtcgcg aaggccgtgg
     2281 ccgtcgacct gggcgtctcc gccgacgagc tggtgccgca catggtcggg gccgccacca
     2341 tcgccgcgct cgacgcgatg ggccgccggg ccaaggaccc cggggccgcc gacttccagg
     2401 ccacggcgct caccgtggtc gacgacgcca tggccttcat cggcggcggc gtgcaggccc
     2461 tggccgaccg ctagcggccg ggcgcgcccg ccagctcgtt gtggcgggct tcgcgtgagg
     2521 cgttcagcgg cacgggctgg acggacacgc ggcgcttgcc ggccgcgatc acctggatcg
     2581 cggtgcgctt gggaccccac ttcacggcgg cgggcccgac gggcagcagg gcctgccagg
     2641 tcggcgggtc catgcggccg ctggccacca ggccgcgcga ggtctggaag gcgcgcaccg
     2701 cggtctcggt cccgctgccg tagttgccgt tcaccgtccg cagcaggcct gccttcatca
     2761 ggtgctgctg gagccagacc accaggtccg agcggtagcc gcgcttcagg agggggtagg
     2821 tgtcgcgccg cacgggcgtc ccgaacggcg agaacggcga gcccagcgcg gtccactggc
     2881 gcgcgcccga gaactgccac acccaccagg acaggcccgt ggcgccgtag gttccggcca
     2941 gcagccggaa gcggcgcacg tcggcggcct tcgggttgga gtaggtctgg cccagcgggt
     3001 agatcggccg gccgtagacc ttgttgtagg tccaggtgtt gcggtagatc gtgtcgaccg
     3061 aggtcccgat cgtcttccag tacatctggg gcaggttcgc ctgcgcgccg cccgggccca
     3121 tgaacaccga gtaggggaac ccggggtggt agtgcacgta cgggaagctg gtcagcgcca
     3181 cctcgtagtc cggcccgacc ttgcggcgca gccgcgccat gtaggtctgg gccgagacgt
     3241 acttgccctc gtagtggccc tcggcgtcga tgaccgtgca gtccgcgccc gcgcgcgccg
     3301 cctcggcggc gcggtcggcc tc
//
LOCUS       DASUPW010000101         2451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_992841, whole genome shotgun sequence.
ACCESSION   DASUPW010000101 DASUPW010000000
VERSION     DASUPW010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992841"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="VFY44_02325"
     CDS             complement(<1..1145)
                     /locus_tag="VFY44_02325"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5909299.1"
                     /translation="MSEDTDILIVGAGFSGLGTAIRLRQEGYTDFVVLERGDDVGGTW
                     HFNTYPNCGCDVPSHLYSFSFAPNADWSRTYSRQPEIRDYLQRTCDQFGVRPHVRTNT
                     TVLSAEWDEEAGRWNVETDAGSYRARMLIPGMGPLAEPRIPEIEGLEDFQGEVMHSAR
                     WDHSVDLKGKKVASIGTGASAIQYVPAIQPDVEQLHVFQRTAPWIMPHTDRPTKSWER
                     RIYGAFPALEKLVRGGVYAARELLVVGFVKQPKLMGLVEKIARKHIQQSVPDPELRKK
                     VTPDYTIGCKRILPSNKWYPALGKDNVELVTDGVVKATATGVVDAAGVEREADVIIFG
                     TGFQVTDMPVGKAVRGRGGKTMDEAWQGSPSAYLGTSIPGFPNLFMLL"
     gene            1189..1785
                     /locus_tag="VFY44_02330"
     CDS             1189..1785
                     /locus_tag="VFY44_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5909300.1"
                     /translation="MATKSPRMTGADRRQQLLDVTKAIVAADGFHAVSIERVAREAGI
                     TRPVVYGHFGDLEGLLEALIDRETLRSISQLEDVLRYDLAEGDQVETLLASLRGYLEA
                     VRADPDTWRLVLMPQEGAPPSLHERIAAGRAAVVAQLARNTAAGFRPGDESPDPELTA
                     HMLSAVSDEGARLLLTDPDRYSLDRILTHARWLLERLQ"
     gene            complement(1779..>2451)
                     /locus_tag="VFY44_02335"
     CDS             complement(1779..>2451)
                     /locus_tag="VFY44_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX5909301.1"
                     /translation="DHVLVVARLRGEDGALGLPALAIVDVDAPGFTRTQIPMPNVGPD
                     KQWQLFFDDVEVTEDRLVGGRTGGLGAVFDGLNPERIMGASVTQGAGRRALAMAADYA
                     RERRVWNGVPIGTHQGVSHPLAEAKIELELARLMTQKAAVLYDTGAKGAGEASNMAKY
                     AAAEASIKCVDQAIQTHGGNGFALEYGLAQMWWGVRLTRTAPVSREMILNYVAEHSLG
                     LPKSY"
BASE COUNT          339 a          867 c          875 g          370 t
ORIGIN      
        1 agcagcatga acaggttggg aaagcccggg atcgacgtgc ccaggtaggc ggacgggctg
       61 ccctgccagg cctcgtccat ggtcttgccc ccgcggccgc gcacggcctt gcccaccggc
      121 atgtcggtca cctggaagcc ggtgccgaag atgatcacgt cggcctcgcg ctccacgccg
      181 gccgcgtcga ccacaccggt ggccgtggcc ttgaccacgc cgtcggtcac cagctccacg
      241 ttgtccttgc ccagggcggg gtaccacttg ttcgagggca ggatccgctt gcagccgatg
      301 gtgtagtcgg gcgtgacctt cttgcgcagc tccgggtcgg gcacggactg ctggatgtgc
      361 ttgcgcgcga tcttctccac gaggcccatc agcttcggct gcttgacgaa gccgacgacc
      421 aggagctcgc gggcggcgta gacgcccccg cgcaccagct tctccagcgc cgggaaggcg
      481 ccgtagatcc gccgctccca ggacttggtg gggcggtcgg tgtggggcat gatccagggc
      541 gccgtgcgct ggaagacgtg cagctgctcg acgtcgggct ggatcgcggg cacgtactgg
      601 atggccgacg cgccggtgcc gatcgaggcc accttcttgc ccttcaggtc gaccgagtgg
      661 tcccagcggg cggagtgcat gacctcgccc tggaagtcct cgagcccctc gatctccggg
      721 atccgcggct cggccagcgg gcccatgccg gggatcagca tgcgggcgcg gtagctgccg
      781 gcgtcggtct cgacgttcca gcgccccgcc tcctcgtccc actcggcgga caggaccgtg
      841 gtgttcgtgc gcacgtgggg gcggacgccg aactggtcgc acgtgcgctg gaggtagtcg
      901 cggatctcgg gctggcggga gtaggtgcgc gaccagtccg cgttgggcgc gaacgagaac
      961 gagtacaggt gcgagggcac gtcgcagccg cagttgggat acgtgttgaa gtgccacgtg
     1021 ccgcccacgt cgtcgccgcg ctcgagcacg acgaagtcgg tgtagccctc ctggcgcagc
     1081 cggattgcgg ttcccagccc ggagaagccc gcgccgacga tcaggatgtc tgtgtcctcg
     1141 ctcattacag gagcgtaact tacagtggtg taaggtgcgt ttcaacccat ggcgacgaag
     1201 tcacccagga tgacgggcgc cgacaggcgc cagcagctgc tggacgtgac gaaggcgatc
     1261 gtcgccgcgg acgggttcca cgccgtgtcg atcgagcgcg tggcgcgcga ggccggcatc
     1321 acgcggcccg tggtctacgg ccacttcggg gacctcgagg ggctgctcga ggcgctgatc
     1381 gaccgcgaga ccctgcgctc gatcagccag ctcgaggacg tcctgcgcta cgacctggcc
     1441 gagggcgacc aggtcgagac gctgctggcc tcgctacgcg gatacctcga ggcggtgcgc
     1501 gccgaccccg acacctggcg gctcgtcctg atgccccagg agggggcccc gccgagcctg
     1561 cacgagcgga tcgccgccgg ccgcgcggcg gtcgtggcgc agctggccag gaacaccgcg
     1621 gccggcttcc gtcccgggga cgagagcccc gatccggagc tgaccgccca catgctcagc
     1681 gcggtgtccg acgagggcgc ccgcctgctg ctgaccgacc cggaccgcta ctcgctggac
     1741 cggatcctga cccacgcccg ctggctgctg gagcggcttc agtaggactt gggcaggccg
     1801 agcgagtgct cggccacgta gttcaggatc atctcgcgcg acaccggggc cgtccgcgtc
     1861 aggcgcacgc cccaccacat ctgggccagg ccgtactcca gcgcgaagcc gttgccgccg
     1921 tgggtctgga tcgcctggtc cacgcacttg atcgaggcct cggcggccgc gtacttggcc
     1981 atgttcgagg cctcgcccgc gcccttggcg ccggtgtcgt agagcaccgc cgccttctgg
     2041 gtcatcagcc gcgccagctc cagctcgatc ttggcctcgg ccagcgggtg cgacacgccc
     2101 tggtgggtgc cgatcggcac gccgttccac acccggcgct cgcgcgcgta gtcggcggcc
     2161 atggccagcg cgcggcggcc ggccccctgg gtcaccgagg cgcccatgat ccgctcgggg
     2221 ttcaggccgt cgaacaccgc gcccaggccg ccggtgcggc cgccgaccag gcggtcctcg
     2281 gtcacctcga cgtcgtcgaa gaacagctgc cactgcttgt cggggcccac gttgggcatg
     2341 gggatctgcg tgcgggtgaa gccgggcgcg tccacgtcca cgatggccag cgcgggcagg
     2401 cccagcgcgc cgtcctcgcc gcgcaggcgc gccaccacca gcacgtggtc g
//
LOCUS       DASUPW010000102         6570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1547348, whole genome shotgun sequence.
ACCESSION   DASUPW010000102 DASUPW010000000
VERSION     DASUPW010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547348"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="VFY44_02340"
     CDS             <1..1107
                     /locus_tag="VFY44_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5909302.1"
                     /translation="LVYELDRECRAAAVKAAHRAGLRAPAALFVNAEPEALDVTRGGA
                     HFDASRIPEGMRVVVEITERAVTARVADLLPIAEQLRRRGVGIALDDVGADRRSLALM
                     PFLRPDVIKLDLRLVQDPPSARTAEIHNAVAAHAERYGAVVVAEGIETEEHAARARAM
                     GATVGQGWLYGRPGPLATERPEPKAAVPISPPTFEDALTPYAAIDGARPTRRASKRLL
                     LSISRELEQRALALGDSAVLLSTFQHERFFGSSQASRYARLASQLAFVGAFGAGVDTF
                     PASAVRGANLEPSDPLVEEWDVVVVDPHFAGALTARDLGDTGCPDLDRRFDFKLTHDR
                     DLVVRAARSLMARIDPTHRASDHLAGRSASAQPG"
     gene            1325..2107
                     /locus_tag="VFY44_02345"
     CDS             1325..2107
                     /locus_tag="VFY44_02345"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5909303.1"
                     /translation="MTDTRADSRGGTAEKPFDLTFPAGEPGEYDQDEEFCEIVIDGET
                     RRIRFHDYDEIFKIPGLYEQLFYAEMECDSPRTIAGLIGEQVDNDTASELRVLDVGAG
                     NGMVGEELDRMGAAHIVGVDIIPEAAEAAERDRPGVYDDYFVVDLTDVPTGELARMEA
                     HKFNCLTTVAALGFGDIPPEAFTTAFNLVENGGLVAFNIKERFVTDGDRTGFEDLIST
                     ALDDGTLELEAQRRYRHRLDVAGHPLYYIAMVARKRADLPTA"
     gene            complement(2104..2667)
                     /gene="pyrE"
                     /locus_tag="VFY44_02350"
     CDS             complement(2104..2667)
                     /gene="pyrE"
                     /locus_tag="VFY44_02350"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX5909304.1"
                     /translation="MSDLSPASERLLAALREHALVIGKVTLSSGAEAEYYVDAKRATL
                     LPDGFRAMGELVAAKAAETGATAVGGMTMGADPVACAALAAGADVKAFFVRKDVKAHG
                     LQRGIEGPLLDPESDRCLIVEDVVTTGSATLAAIDRVKEAGLQIVGVVSVLDRLAGGA
                     EAIEQASGAPYTPLTTIDDVYPERPDR"
     gene            2693..3124
                     /locus_tag="VFY44_02355"
     CDS             2693..3124
                     /locus_tag="VFY44_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909305.1"
                     /translation="MPSLVRLLAITASAVLGLSLLIFALDQSGEGRDTQLRSLQGADA
                     TTVVRSQEDLDVPKPAPEVERVREQEHSSARELIDDGNDILVTPFTGIVGTDANIWIQ
                     RLVTAGLGLALFGFGGLMLAAWLPQKEAAHTDWREAGSGTG"
     gene            complement(3254..4255)
                     /locus_tag="VFY44_02360"
     CDS             complement(3254..4255)
                     /locus_tag="VFY44_02360"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX5909306.1"
                     /translation="MRRQLTLSTVATVALLGLPAAAQAQLPTPAPPAPTPPPSVTPPA
                     QKGKVSIKVSGGQATKKARYVARGAAIRVRGTVKPLVAGQTVLVEVLRKKKVISRQSV
                     PVGAKGRVRARIKTRRAGSIKVRIRHVATPQQLAFTSRSARVKVVSLSAGQGSRGTKV
                     LLLQRGLYKLGYAVPRTGFYDGGTSRAVLAFRKTNSMGRDGFATRGVYSRVLQGKGRF
                     KARFPKAGRHVEFDWSRQVLVLFNSKGKASRTYHASSGKPSTPTVFGRFRFYRRDLGT
                     NAKGMVDAAYFIGGYAIHGYASVPNYPASHGCIRVPVPNARQIRDAVSLGEQIFVYR"
     gene            complement(4252..5115)
                     /locus_tag="VFY44_02365"
     CDS             complement(4252..5115)
                     /locus_tag="VFY44_02365"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX5909307.1"
                     /translation="MAPPVAPPDPPELPEGAERRPPWPAMFALWGFVVGLGTTLVAGT
                     MLAAVVVALGGKADGTAVTVIGTVAQGFCFAGAAWFFASRVAKPRLWHFGLQGARFWP
                     TVGWAALGILTFYVITLIYGALVQPDAEQETVEALGGDQGWFGLIVAGTMVIAVAPVV
                     EEFFFRGFFYRALRSRMSIAVAALVDGAVFGIIHFQFDGVDGLLILPPLAVLGVLFCL
                     VYEKTGSLLAPIGMHAFNNAVAFGVQADDGWMVSVVVAPLVFAALVVAGRRLPRGPVA
                     SPARASTVSRA"
     gene            complement(5131..5799)
                     /locus_tag="VFY44_02370"
     CDS             complement(5131..5799)
                     /locus_tag="VFY44_02370"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5909308.1"
                     /translation="MPRAVLFDALGTLVRLEPPAPRLRAELTRVAGVDVGDRAAERGF
                     AAEIAFYLRNHMRGGDAAGLEALRDDCAAVLHEALGVPGLERAAVRQAMLASLVFTAF
                     PDAAPALRELRGAGARVVVVSNWDRSLGRALDQAGLGGLVDGVVSSAEVGRAKPAPEP
                     FTAGLELAGARAEDALFVGDSPDTDIEGARAAGIRALLVAREGPAPEGVEAVASLERV
                     PSLF"
     gene            complement(5792..>6570)
                     /locus_tag="VFY44_02375"
     CDS             complement(5792..>6570)
                     /locus_tag="VFY44_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909309.1"
                     /translation="LCAFPASVFLGAPYSESLFLAASVGAFYAARTGHWAAAGAAAAA
                     ASATRSAGVLLLVPLAILYWQGTEPGHRLRPHLAWLALAPGGLVAYAAYLAVAHGDPL
                     AFSSAQELWSRHFAGPFVGAWDGLVAAFEGARQLGSGSRETVYFADAGGDPFRVAAIN
                     LMLFGSLVFAAIASLGVLRRLPFAYGAYVVVALAVPLSYPVGPQPLMSLPRFLVVLFP
                     VFMWMGVACAERGNTARVAAASALGLGLFTAQYATWYFIA"
BASE COUNT          993 a         2409 c         2343 g          825 t
ORIGIN      
        1 ctggtgtacg agctcgatcg cgagtgcagg gccgccgcgg tcaaggcggc gcaccgggcc
       61 gggctccgcg cgccggccgc cctgttcgtg aacgccgaac ccgaggcgct cgacgtgacc
      121 cggggtggcg cgcacttcga cgcctcccgg atccccgagg ggatgcgggt ggtggtggag
      181 atcaccgagc gcgccgtgac cgcgcgggtc gccgacctcc tgccgatcgc cgagcagctc
      241 cggcgccgcg gcgtcggcat cgcgctggac gacgtcggcg cggatcgtcg ctccctggca
      301 ctgatgccct tcctgcgccc ggacgtgatc aagctcgacc tccgcctcgt acaggacccg
      361 ccgagcgcga ggaccgctga gatccacaac gccgtggcgg cccacgccga gcggtacggc
      421 gcggtggtgg tggccgaggg catcgagacc gaggagcacg ccgcccgggc acgcgcgatg
      481 ggcgccaccg tcggccaggg gtggctgtac gggcgccccg ggccactcgc cacggaacgc
      541 cccgagccga aggccgcggt ccccatctcc ccgcccacgt tcgaggacgc cctgacgccg
      601 tacgcggcga tcgacggcgc gcgccccacg cgccgtgcca gcaagcgcct gctgctgtcc
      661 atcagccgcg agcttgagca acgcgcgctg gcgcttggtg acagcgcggt cctgctcagc
      721 acgttccagc acgagcgctt cttcggctcg agccaggcga gccggtatgc gcgcctggcg
      781 tcgcagctgg ccttcgtcgg cgccttcggt gccggcgtgg acacgtttcc ggcgtcggcg
      841 gtccgcggcg cgaacctgga gccctccgat ccgctggtgg aggagtggga tgtggtcgtc
      901 gtcgaccctc atttcgcggg cgccctgacc gcgcgcgacc tcggcgacac gggctgcccg
      961 gacctggacc gccggttcga cttcaagctc acccatgacc gcgatctggt ggtccgcgcc
     1021 gcccgctcgc tgatggcccg tatcgaccct acgcaccggg cctcggacca cctggccggc
     1081 cgctccgcca gcgcgcaacc tggctagccg gcgcgtttcc gaacctcccg atcccgctct
     1141 gagagcggaa aagttaggga ggcgccgatt ctcggtaacc ttttcctcga cgcttgcgga
     1201 gcgctcagcg ccgatcagcg gctatctggc gcgaaaacgt tctgctcaaa gcgaactccc
     1261 caccggcacc aacgcattcc ggggagacgg acttacgaga ccaatcaagg aggcaggacc
     1321 ccacgtgacc gacactcgtg ctgattccag gggaggaacc gctgagaagc cgttcgatct
     1381 cacctttccc gcaggcgagc ccggcgaata cgaccaggac gaggagttct gcgagatcgt
     1441 catcgacggg gagacacgtc ggatccgctt ccacgactac gacgagatct tcaagatccc
     1501 gggtctctac gagcagctgt tctacgccga gatggagtgc gactcgccgc gaaccatcgc
     1561 cggcctgatc ggcgagcagg tggacaacga caccgcgtcc gagctgcgcg tgctcgacgt
     1621 gggcgccggc aacgggatgg tcggcgagga gctcgaccgc atgggcgccg cgcacatcgt
     1681 gggcgtcgac atcatccccg aggccgccga ggccgccgag cgcgacaggc cgggcgtgta
     1741 tgacgactac ttcgtcgtgg acctgaccga cgtgcccacc ggcgagctgg cccggatgga
     1801 ggcgcacaag ttcaactgcc tgaccacggt cgcggcgctg ggcttcggcg acatcccgcc
     1861 ggaggccttc accaccgcct tcaacctggt cgagaacggc ggcctggtcg cgttcaacat
     1921 caaggagcgg ttcgtgaccg acggcgaccg cacgggcttc gaggatctga tctcgaccgc
     1981 cctggacgac ggcaccctcg agctcgaggc ccagcggcgc taccgccacc gcctggacgt
     2041 cgccggccac ccgctgtact acatcgccat ggtggcgcgt aagcgcgccg acctgccgac
     2101 cgcctagcgg tccgggcgct ccgggtacac gtcatcgatg gtcgtcaggg gcgtgtaggg
     2161 cgcccctgac gcctgctcga tcgcctcggc cccgccggcc aggcggtcga gcacgctgac
     2221 gacccccacg atctgcaggc ccgcctcctt gacgcggtcg atcgcggcca gggtggcgct
     2281 gccggtggtc acgacgtcct ccacgatcag gcagcggtcg ctctcggggt ccagcagcgg
     2341 cccctcgatc ccgcgctgca ggccgtgggc cttgacgtcc ttgcgcacga agaaggcctt
     2401 gacgtcggcg cccgcggcga gcgcggcgca ggccaccggg tcggcgccca tggtcatgcc
     2461 cccgaccgcc gtggcccccg tctcggccgc cttggcggcc accagctcgc ccatggccct
     2521 gaacccgtcg ggcagcagcg tggcgcgctt ggcgtccacg tagtactcgg cctcggcgcc
     2581 gctggagagg gtaaccttgc cgatgaccag cgcgtgctcg cgcagggcgg ccaggaggcg
     2641 ttcggatgcg ggactcaggt cggacaccgg gtctgccgat ggtattggag tcatgccttc
     2701 cctcgtacgc ctgctcgcca tcacagcctc ggccgtgctc ggcctcagcc tgctcatctt
     2761 cgccctcgat cagtccggtg aggggcgcga cacccagttg cgcagcctcc agggcgcgga
     2821 cgcgaccacg gtggtccgct cgcaggagga cctggacgtg cccaagccgg cccccgaggt
     2881 cgaacgcgtc cgcgagcagg agcactcctc cgcccgcgag ctcatcgacg acggcaacga
     2941 catcctggtg accccgttca ccggcatcgt cggcacggac gccaacatct ggatccagcg
     3001 cctggtcacc gccgggctgg gcctggcgct gttcggcttc ggcggcctga tgctggccgc
     3061 ctggctgcct cagaaggagg cagcccacac cgactggcgc gaagccggct ccggcaccgg
     3121 ctagacccct gcaaaccaac gccgggcggc gtcatcggca acctcgtgtg cgtgcgcaca
     3181 cgtcggttac ctcttccttg cccgggcggg gttttcggtg cctaaccggc accgggaacg
     3241 ttgggcctgc ccgtcagcga tacacgaaga tctgctcgcc caaggagacc gcgtcgcgga
     3301 tctggcgggc gttcggcacc ggcacccgga tgcagccgtg gctggccggg tagttgggca
     3361 ccgaggcgta gccgtggatg gcgtacccgc cgatgaagta ggcggcgtcg accatgccct
     3421 tggcgttggt gcccaggtca cggcggtaga agcggaagcg gccgaacacc gtgggggtgg
     3481 acggcttgcc cgacgacgcg tggtaggtgc gtgaggcctt gcccttggag ttgaacagca
     3541 ccagcacctg gcgcgaccag tcgaactcga cgtggcggcc ggccttgggg aagcgggcct
     3601 tgaagcggcc cttgccctgg agcaccctgg agtagacgcc gcgggtggcg aagccgtcgc
     3661 gtcccatgct gttggtcttg cggaaggcga gcacggcgcg cgaggtgccg ccgtcgtaga
     3721 agccggtgcg cgggacggcg tagccgagct tgtagaggcc gcgctggagc agcagcacct
     3781 tggtgccgcg tgagccctgg ccggcgctga gcgagacgac cttgacgcgc gcggagcggg
     3841 acgtgaacgc cagctgctgc ggggtcgcca cgtggcggat gcggaccttg atgctgcccg
     3901 ctcggcgggt cttgatgcgg gcgcgcacgc ggcccttggc gccgacgggg acggactggc
     3961 gcgagatgac cttcttcttg cgcaggacct cgaccagcac ggtctggccg gcgaccagcg
     4021 gcttgacggt gccgcggacg cggatcgcgg caccgcgggc cacgtagcgg gccttcttgg
     4081 tggcctggcc gcccgagacc ttgatcgaga ccttgccctt ctgggcgggc ggcgtgaccg
     4141 agggcggcgg cgtgggcgcc ggcggggccg gtgtcggcag ctgggcctgg gcggctgcgg
     4201 gcaggcccag gagggcgacg gtcgcgacgg tggagagtgt gagttggcgc ctcatgcgcg
     4261 agaaactgta ctggcgcgtg cgggagaggc gaccggcccc cgcggaaggc ggcggcccgc
     4321 gaccacgagc gcggcgaaga ccagcggggc gaccacgacg gagaccatcc agccgtcgtc
     4381 ggcctggacg ccgaaggcga ccgcgttgtt gaacgcgtgc atgccgatgg gcgccagcag
     4441 cgagccggtc ttctcgtaga ccaggcagaa caacaccccg agcacggcca gcggcggcag
     4501 gatcagcaac ccgtcgacgc cgtcgaactg gaagtggatg atcccgaaca cggcgccgtc
     4561 caccagcgcg gcgaccgcga tggacatccg gctgcgcagg gcgcggtaga agaagccgcg
     4621 gaagaagaac tcctcgacca cgggcgccac cgcgatgacc atcgtcccgg ccacgatcag
     4681 cccgaaccag ccctggtcgc cgcccagcgc ctccacggtc tcctgctcgg cgtcgggctg
     4741 gacgagcgcc ccgtagatca atgtgatcac gtagaaggtc aggatgccca gcgcggccca
     4801 gccgaccgtg ggccagaagc gcgcgccctg gaggccgaag tgccacagcc gcggcttggc
     4861 cacgcggctg gcgaagaacc aggccgcgcc ggcgaagcag aagccctggg cgaccgtgcc
     4921 gatcaccgtc acggccgtgc cgtcggcctt gccgccgagt gcgaccacga ccgccgccag
     4981 catggtcccg gccacgagag tggtccccag gcccacgacg aagccccaca gcgcgaacat
     5041 cgcgggccac ggcggcctgc gctcggcacc ctccggcagc tcgggcgggt ccggcggcgc
     5101 gacgggagga gccatggacg ccatccgcgc tcagaatagg gagggcacgc gctccagcga
     5161 tgcgaccgcc tcgacgccct ccggcgccgg cccctcgcgc gccaccagca gtgccctgat
     5221 cccggcggcg cgggcgccct cgatgtcggt gtccggcgag tccccgacga acagcgcgtc
     5281 ctcggcccgg gctccggcca gctccagccc cgccgtgaac ggctcgggcg ccggcttcgc
     5341 ccgtcccacc tccgccgagg agaccacccc gtcgaccagc ccgccgagcc ccgcctggtc
     5401 cagcgcccgc cccagcgagc ggtcccagtt gctgaccacc accacgcgcg cccccgcgcc
     5461 gcgcagctcc cgcagcgcgg gagccgcatc ggggaatgcg gtgaacacca gcgacgccag
     5521 catggcctgg cggaccgccg cccgctccag gccgggcacg cccagcgcct cgtgcaggac
     5581 ggccgcgcag tcgtcgcgca gcgcctcgag gccggccgcg tcgccgccgc gcatgtggtt
     5641 gcgcaggtag aaggcgatct cggcggcgaa tccgcgctcg gcggcccggt cgccgacgtc
     5701 gacgccggcg acgcgcgtca gctcggcccg gagccgtggc gccggcggct ccaggcgcac
     5761 cagcgtgccg agcgcgtcga agaggacggc cctaggcaat gaagtaccag gtggcgtact
     5821 gggccgtgaa cagcccgagg ccgagcgccg aggcggcggc gacccgcgcc gtgttcccgc
     5881 gctcggcgca cgccaccccc atccacatga agaccgggaa cagcaccacc aggaaccgcg
     5941 gcaggctcat cagcggctgc gggccgaccg ggtagctcag cgggacggcc agcgccacga
     6001 ccacgtaggc gccgtaggcg aacggcagcc ggcgcagcac ccccagcgag gcgatggcgg
     6061 cgaacaccag cgagccgaac agcatcaggt tgatcgccgc cacccggaac gggtccccgc
     6121 ccgcgtccgc gaagtagacg gtctcgcgcg agcccgaccc cagctggcgc gcgccctcga
     6181 acgctgcgac cagcccgtcc cacgccccca cgaacgggcc ggcgaagtgg cgcgaccaca
     6241 gctcctgggc cgaggagaag gccagcgggt cgccgtgcgc gacggcgagg tacgccgcgt
     6301 acgccaccag gccgcccggc gccagcgcca gccacgccag gtgcgggcgc aggcggtggc
     6361 cgggctcggt gccctgccag tagaggatgg ccagcggcac caacaggagg acgccggcgc
     6421 tgcgggtcgc actggctgcc gccgccgcgg cccctgccgc cgcccagtgg cccgtgcggg
     6481 cggcgtagaa cgcgccgacg gatgcggcca ggaacaggct ctcggagtac ggcgcgccca
     6541 ggaacaccga cgcgggaaac gcgcacagca
//
LOCUS       DASUPW010000103         3369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_162902, whole genome shotgun sequence.
ACCESSION   DASUPW010000103 DASUPW010000000
VERSION     DASUPW010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162902"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1396)
                     /locus_tag="VFY44_02380"
     CDS             complement(173..1396)
                     /locus_tag="VFY44_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX5909310.1"
                     /translation="MSAFARERRFWTITGWAAVMGLVMGAVGIGWLLLVEELPEELWG
                     HPDDHDFAGGRLWWIPLATGFGLLVGLLRRWITAARQPDLTAEIGEAHVDRKVVPASV
                     GVSAVSLMGGASLGPEGGLGLLGGGLGDWLAKRTGADPETRKAHTLTGMAGAFGGLFT
                     APLLGPLMISEIARPGGDRPVERLIPTVVASTLGFAVLYPVIGHTFLDLYDVPGYDLE
                     VWHMFAGVGIGAMGAVLAVVTGIAMKAGARVAGRVSSHPVPLAMAGGLFIGLMAYLVP
                     LTLFSGSGELKVIVNHGPALGAGLLIAVVLAKILTFAVSLSTGFIGGTIFPMLFIGGT
                     AGMALHVIVPGIPAGLAVPAAMAAVPGAAVAIPFSLIAIIAFAVTVDAANVAPIGIAV
                     LTAYLLVVGTGLIKS"
     gene            complement(1400..2074)
                     /locus_tag="VFY44_02385"
     CDS             complement(1400..2074)
                     /locus_tag="VFY44_02385"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEX5909311.1"
                     /translation="MAPADVPGRRQRFGLVLIAITASFIVEGTLSDSAWAQGVVTALL
                     GTTLLLTFWAADMPPHRLRVAAVAVAVVIAAGIATVAIGDHTAEGIGRLMNAALVAFA
                     PPMLVVGMLRSVRRHRAVTLEAVLGGLALYVLVGMLFAFVFGAVDRLGGEPFFANGVQ
                     ATEPRCNYFSFSTLTTVGFGDLTARSDLGRTLAVLEALVGQLYLVTVVSVGVSNLVPR
                     ARAGRG"
     gene            complement(2074..3138)
                     /locus_tag="VFY44_02390"
     CDS             complement(2074..3138)
                     /locus_tag="VFY44_02390"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5909312.1"
                     /translation="MTEPPPQRVEVNIGLRTMLLAVAVAAAVGLAIVSLGSLLSIFVA
                     GVLALGLDPVVSRLVRRGWPRGRAALVVFAGLFVGVFLIVAVTVGPLWDQVKEFVEKL
                     PEYWDQLTETAAFDKLTSSGTEDSVRSALEDLAKGLPDAANTLLGIAGGVFGSVLSLV
                     TLSFLALFLLMERPTITEWLFGFAPPEAERRWRPILEESIDAISDSLLGNIAISLVAA
                     TVAGLSAWAFGLPFPIVLAVIVGFLDLIPQVGATVAGVILVLVALTVSTPAAIAMAVI
                     QLIYQQIENYVVYPLVYRKAVSLSPFTTIVAVLIASSILGVVGAILAVPFAAVIKIVI
                     REAGRPRRERMATARAAGEG"
     gene            complement(3190..>3369)
                     /locus_tag="VFY44_02395"
     CDS             complement(3190..>3369)
                     /locus_tag="VFY44_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909313.1"
                     /translation="MSGGIFGRSVGIAVASLTAIGALLGVGGAYPFWSLGVFAICIVV
                     IHGLAVYDPEPELAR"
BASE COUNT          582 a         1257 c         1180 g          350 t
ORIGIN      
        1 agcagcacgg caccggccag cgacgtgacg gccgccgcca cggcgaaccc gcccggatgg
       61 tcgaacacca gccccaggag gccgacggga gcgaccacgc gcgccgcgcc gaaggcgatg
      121 aacgccagcg ccgggagcgc cagcgccagc gaggtccggc cgggccggcg gctcacgact
      181 tgatcaggcc ggtgcccacc accagcaggt aggcggtcag caccgcgatc ccgatggggg
      241 cgacgttggc cgcgtccacg gtgacggcga acgcgatgat ggcgatcagg ctgaacggga
      301 tggccaccgc cgcgccgggg acggcggcca tcgcggcggg gacggcgagc ccggccggga
      361 tgccggggac gatcacgtgc agcgccatgc ccgcagtgcc gccgatgaac agcatcggga
      421 agatcgtgcc gccgatgaag cccgtcgaga ggctgacggc gaacgtcagg atcttcgcca
      481 ggacgacggc gatcagcagg cctgcgccca gcgccggccc gtggttgacg atgaccttga
      541 gctccccgga gccggagaac agcgtgagcg ggaccaggta ggccatcagc ccgatgaaca
      601 gcccgcccgc catggccagg ggcacggggt gcgacgacac acgcccggcc acgcgcgcgc
      661 ccgccttcat ggcgatgccg gtcaccacgg ccaggacggc gcccatcgcg ccgatgccca
      721 cgcccgcgaa catgtgccac acctcgaggt cgtagccggg cacgtcgtac aggtccagga
      781 aggtgtgccc gatcacgggg tagagcacgg cgaacccgag cgtcgaggcg accacggtgg
      841 ggatgagccg ctcgaccggg cggtcgccgc ccggccgcgc gatctcggag atcatcagcg
      901 gccccagcag cggcgcggtg aacagcccgc cgaacgcgcc cgccatgccg gtgagcgtgt
      961 gggccttacg ggtttcgggg tcggcgccgg tgcgcttggc gagccagtcg cccaggccgc
     1021 cgccgagcaa gccgaggccg ccctcgggcc cgaggctggc gccgcccatc agcgacaccg
     1081 ccgagacgcc gaccgaggcc ggcaccacct tgcggtccac gtgcgcctcg ccgatctcgg
     1141 cggtcaggtc gggttggcgg gccgccgtga tccaccggcg cagcaggccc accaggagcc
     1201 cgaagcccgt ggccaggggg atccaccaca gcctgccgcc cgcgaagtca tggtcatcgg
     1261 gatgccccca cagctcctcc ggcaactcct cgaccaggag cagccagccg atgcccaccg
     1321 cacccatgac caggcccatc acggcggccc atccggtgat cgtccaaaac ctgcgctcgc
     1381 gggcgaaggc gctcaccggt caccccctgc cggcacgggc gcggggcacg aggttcgaca
     1441 cgcccacgga gaccaccgtg accaggtaga gctggcccac gagcgcctcc agcaccgcca
     1501 gcgtgcgccc gaggtcgctg cgggcggtca ggtcgccgaa gccgacggtg gtcagcgtgg
     1561 agaagctgaa gtagttgcag cggggctccg tggcctggac cccgttggcg aagaacggct
     1621 cgccgccgag gcgatcgacc gcaccgaaca cgaacgcgaa cagcatcccc acgagcacgt
     1681 acagagccag cccgccgagc acggcctcga gggtcacggc ccggtgccgg cgcacgctcc
     1741 ggagcatccc caccacgagc atgggcgggg cgaaggccac gagcgcggcg ttcatgagcc
     1801 gcccgatccc ctcggccgtg tggtcgccga tggccacggt cgcgatcccg gccgcgatga
     1861 ccacggccac ggccaccgcc gccacccgga gccggtgggg cggcatgtcc gccgcccaga
     1921 acgtgagcag cagggtcgtg cccagcagcg cggtcaccac cccctgggcc caggcgctgt
     1981 ccgagagggt cccctcgacg atgaacgagg ccgtgatggc gatcagcacc aggccgaagc
     2041 gctggcgcct ccccggcacg tcggcaggcg ccatcaaccc tcgccggccg cgcgcgcggt
     2101 cgccatgcgc tcgcgtcgcg gccggccggc ctcgcggatg acgatcttga tcaccgcggc
     2161 gaacgggacc gccaggatcg cgccgaccac tcccaggatc gagctggcta tgagcacggc
     2221 gacgatggtc gtgaacggcg acagcgacac cgccttgcgg tagacgagcg ggtagaccac
     2281 gtagttctcg atctgctggt agatcagctg gatcaccgcc atcgcgatgg ccgccggggt
     2341 gctcaccgtg agcgccacga gcacgaggat cacgcctgcg accgtggccc ccacctgcgg
     2401 gatcaggtcc aggaagccga cgatgaccgc caggacgatc gggaacggca ggccgaaggc
     2461 ccaggccgac aggccggcga ccgtcgcggc cacgagcgag atcgcgatgt tgcccagcag
     2521 cgagtcggag atcgcgtcga tcgactcctc caggatcggg cgccagcgcc gctcggcctc
     2581 gggtggcgcg aacccgaaca gccactcggt gatcgtcggg cgctccatca gcaggaacag
     2641 cgccaggaac gacagcgtga ccagcgacag caccgagccg aacacgccgc cggcgatgcc
     2701 gagcagcgtg ttggccgcgt cggggaggcc cttggccagg tcctcgagcg ccgaccgcac
     2761 cgagtcctcg gtgccgctgc tggtgagctt gtcgaacgcc gcggtctccg tcagctggtc
     2821 ccagtactcg ggcagcttct ccacgaactc cttgacctgg tcccacagcg gcccgaccgt
     2881 gacggccacg atcaggaaca cgcccacgaa cagcccggcg aagaccacga gcgccgcacg
     2941 cccccggggc cagccgcgcc gcaccagacg gctgaccacc gggtccaggc ccagcgccag
     3001 cacgccggcc acgaagatcg acagcagcga ccccagcgag acgatggcca gccccacggc
     3061 ggccgcgacc gcgaccgcca gcagcatcgt ccgcaggccg atgttgacct cgacccgctg
     3121 ggggggtggc tcagtcacgc gttggcaatc tctcacgggg cccggccgga tgccaggccc
     3181 cgcggacgct tatctggcga gctctggctc cgggtcgtag acggccaggc cgtggatgac
     3241 cacgatgcag atcgcgaaca cgccgagcga ccagaacggg taggcgccgc ccacgccgag
     3301 cagcgcgccg atcgccgtga ggctggccac cgcgatcccg acggaacgcc cgaagatccc
     3361 tccgctcat
//
LOCUS       DASUPW010000104         2680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1686292, whole genome shotgun sequence.
ACCESSION   DASUPW010000104 DASUPW010000000
VERSION     DASUPW010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686292"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..1215
                     /gene="nadA"
                     /locus_tag="VFY44_02400"
     CDS             202..1215
                     /gene="nadA"
                     /locus_tag="VFY44_02400"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006878.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX5909314.1"
                     /translation="MPPIELPVLQPGLEPALSPGEETLMKDEVRALAAERGAVILAHN
                     YQVPEVQDVADFVGDSLALSRQAAAADEQTIAFCGVHFMAETASILSPEKTVLLPDLD
                     AGCSLADSITADELRTWKAQHPGAVVVMYVNTTAEVKAETDYCCTSSNAVQVVEHIWR
                     EHGPETEILFGPDMFLGAYVEKVTGRRMNVWTGECHVHAGIRPSDISDVRAANPGAEF
                     LIHPECGCSTSVMEYVAAGDVDAENTHMLSTGGMLTHARESEADTFIVATETGMLHPL
                     EQENPDKTFVPANRAAVCQFMKMITLPKLRDALRDGGPVVSVDPSIAERARVPIERMV
                     ALG"
     gene            complement(1219..2019)
                     /locus_tag="VFY44_02405"
     CDS             complement(1219..2019)
                     /locus_tag="VFY44_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909315.1"
                     /translation="MNRVLTACALACAAALTAVAPAHAAKKPAKPKVAKFAASLKGEQ
                     VLTWSYNRPQTGMCSGSESGGGSTRITYSDASATLTAFQPPKSSPLTRLTKGRPFMAN
                     TLKPKATAVREGRHAVVPAPSPPCPAQGGGTGKGPAPQRCGTRTATYRITFDYKKKNT
                     PSLIADPIGGWQGGAESGELRNTFARDNCPSWITGPYEPAEANGAVLRFGVPLKERTL
                     FDRRRRTIKVSASNIECVDARGGFADCKDPGQFKGRIVNLYTLTLKRR"
     gene            2152..>2680
                     /locus_tag="VFY44_02410"
     CDS             2152..>2680
                     /locus_tag="VFY44_02410"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX5909316.1"
                     /translation="MQLERLYPRPGQVEAADAFSRLDLGSRAPEGRPYVVLNMVASLD
                     GKAVLDGTTRGLGGDADREVFHHLRTQADAVMAGAGTVRQERYGRAVKNDALRAKRQD
                     EGLAAEPLTVIVSASMRLPADLPILQAEGAPVAVATGAEGEIEGAVADITYLRTGADL
                     PVLMARLREEHGVRSI"
BASE COUNT          374 a          957 c          951 g          398 t
ORIGIN      
        1 ccgccctcag cccgggcgaa gagaccctga tgaaggacga ggtccgcgcg ctcgctgcca
       61 cagcagcgcg tgcaggcggc cctcgcccac gcgcagcacg acggcgacgg tcacgtgggt
      121 cggaaggctg ttgctattct gttgctccac ggttttcgat cataagtaga aaaccgggcc
      181 cccgtcgaga ggacacgcga gatgcccccg atcgagctcc ccgttcttca gcccggcctc
      241 gagcccgccc tcagcccggg cgaagagacc ctgatgaagg acgaggtccg tgcgctcgcg
      301 gccgagcgag gcgcggtcat cctggcccac aactaccagg tgcccgaggt ccaggacgtg
      361 gccgacttcg tgggcgactc gctggcgctg tcgcggcagg cggcggcggc cgacgagcag
      421 acgatcgcct tctgcggcgt gcacttcatg gccgagacgg ccagcatcct ctcgcccgag
      481 aagaccgtgc tgctgcccga cctggacgcc ggctgctcgc tggccgactc gatcaccgcc
      541 gacgagctgc gcacctggaa ggctcagcac cctggcgccg tggtggtcat gtacgtgaac
      601 accaccgccg aggtcaaagc cgagaccgac tactgctgca cctcctccaa cgccgtccag
      661 gtggtggagc acatctggcg cgagcacggc cccgagaccg agatcctgtt cggccccgac
      721 atgttcctgg gcgcctacgt cgagaaggtc acgggccggc gcatgaacgt gtggaccggc
      781 gagtgccacg tgcacgccgg catccgcccg tcggacatct ccgacgtgcg cgcggccaac
      841 cccggcgccg agttcctgat ccaccccgag tgcggctgct cgacctcggt catggagtac
      901 gtggccgcgg gcgacgtgga cgccgagaac acccacatgc tctccaccgg cggaatgctg
      961 acccacgcgc gggagtcgga ggcggacacg ttcatcgtgg ccaccgagac gggcatgctg
     1021 cacccgctgg agcaggagaa cccggacaag accttcgtgc cggccaaccg cgccgcggtc
     1081 tgccagttca tgaagatgat cacgctgccc aagctgcgcg acgcgctccg ggacggcggg
     1141 cccgtggtct cggtcgatcc gtcgatcgcc gagcgggccc gcgtcccgat cgagcgcatg
     1201 gtcgcgctgg gctgagccct agcgccgctt cagcgtcagc gtgtagaggt tcacgatcct
     1261 ccccttgaac tgtccggggt ccttgcagtc ggcgaacccg ccgcgcgcgt cgacgcactc
     1321 gatgttcgag gccgagacct tgatcgtcct gcgccgcctg tcgaacagcg tcctctcctt
     1381 cagcggaacg ccgaagcgca gcacggcgcc gttggcctcg gcgggctcgt agggccccgt
     1441 gatccagctg gggcagttgt cccgggcgaa ggtgttgcgc agctcgccgc tctctgcgcc
     1501 gccctgccat ccgccgatcg ggtcggcgat caggcttggg gtgttcttct tcttgtagtc
     1561 gaacgtgatc cggtaggtgg ccgtgcgggt gccgcacctc tggggcgccg ggcccttgcc
     1621 ggtgccgccg ccctgggcgg ggcacggggg cgagggggcg ggcaccacgg cgtgcctgcc
     1681 ctcgcgtacc gcggtggcct tgggcttgag cgtgttggcc atgaagggcc ggcccttggt
     1741 cagcctggtg agcgggctcg acttcggcgg ctggaacgcc gtcagggtcg ccgacgcgtc
     1801 cgagtaggtg atgcgggtgc tgccgccgcc gctctcgctg cccgagcaca tgccggtctg
     1861 gggacggttg tagctccagg tcaggacctg ctcgcccttg agcgaggccg cgaacttggc
     1921 caccttgggc ttggcgggct tcttggccgc atgagccggc gccacggcgg tcagcgctgc
     1981 ggcacaggcc agcgcgcagg cggtcaggac acggttcatc gttgctcctc ggtgtggggt
     2041 ggtcgtcatc tggccccagc ctgtggcctg gccccccgcc cggcatcgcg gtggcacccc
     2101 aagaagcacc cctgaccttg ccgggaccgg cgtggcgcta gcgtcctgcc cgtgcagctc
     2161 gagcgcctct acccgcgccc cggccaggtc gaggcggccg acgccttctc ccgcctggac
     2221 ctgggctcac gcgcgcccga gggcaggccc tacgtggtgc tgaacatggt ggccagcctg
     2281 gacggcaagg ccgtgctgga cggcaccacc cgcggcctgg gcggcgacgc cgaccgcgag
     2341 gtgttccacc acctgcgcac ccaggccgac gcggtcatgg ccggggccgg caccgtgcgc
     2401 caggagcgct acgggcgggc ggtcaagaac gacgcgctca gggccaagcg ccaggacgag
     2461 gggctggccg ccgagccgct gaccgtgatc gtcagcgcca gcatgcgcct gcccgccgac
     2521 ctgccgatcc tgcaggccga gggcgcgccc gtggccgtgg ccaccggtgc ggagggcgag
     2581 atcgagggcg cggtggctga catcacctac ctgcggaccg gtgccgacct gcccgtgctg
     2641 atggcgcggc tgcgcgagga gcacggggtc cgctcgatcc
//
LOCUS       DASUPW010000105         2625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1686472, whole genome shotgun sequence.
ACCESSION   DASUPW010000105 DASUPW010000000
VERSION     DASUPW010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686472"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1562
                     /locus_tag="VFY44_02415"
     CDS             120..1562
                     /locus_tag="VFY44_02415"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HEX5909317.1"
                     /translation="MTLAADQVALDDATLTRLGDRADQAEALALRLGLRTFASVTFPV
                     SPEVDPSATILAAREAGERFFCMEQPGRDGYAVCGLGEAIAVTRSGTDRFEGAAADCR
                     RIGARTLEDDQAADPHRPPGAGPVFLGGFAFAATGGATPEWSGFDPAQLVLPEVSIAR
                     MGDQARLTVTVAVDPRQGAAAEALERARRRMAALEPATMPLLDPSPTARPRVAGAAAP
                     SHYEDAVRRAVERIRSGQMSKVVLAREVRVQTGREIDPAPVLDALRGGFPDCYCYCVG
                     GAGGSFVGASPELLVRRDGARAQTVALAGTTRRSADPAVDDHLGEQLRQSPKNRSEQA
                     IVERRIQSTLAPVSVWVAAAEEPVLIKVLNVQHLATPIRAQLSDPVPAVELAGRLHPT
                     PAVGGEPRAVAEPMIPELEGLDRGWYAGAVGWTDLSEDGEFCVALRCALLRGDVAHLY
                     AGCGIVADSVPADELAETEFKLQALLPLLA"
     gene            complement(1576..2439)
                     /locus_tag="VFY44_02420"
     CDS             complement(1576..2439)
                     /locus_tag="VFY44_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909318.1"
                     /translation="MKTTASIPVVAALGLLALAPPALAAPAKLSGGAVSATGATTVKV
                     TNPNRYTLKGTVALASGKTKLASRKVSVKGKRSKKVSLKLSKAGLATLRSKGSLATTM
                     TARLARRGGKAKSYRKALVLKAPGAAGGAPGSGAPGAGQGGSGGGATNRWKATTSMGG
                     TFDLVVENGQVTLAAPTFQPVSCFKSGGYSSAQTSLSSEMFDKLGPWALGNQDATQTQ
                     VVPRVNTLVTSGERTVTYRLNTTRTGDQISGSMVQSFSDSRLEFAFPNPAKIAFISCA
                     GTLNFTAVPAA"
BASE COUNT          311 a          957 c          986 g          371 t
ORIGIN      
        1 ttgggctttt ctgaaattcc gtgccatggg gtggcacgga tttgcacccc atggcaacgc
       61 gtttctgaaa atggggagtg ggggctggag ggcaatggcc tccgcgtaag cttcggagcg
      121 tgaccctcgc cgccgatcag gttgcgctgg acgacgcgac gctcacccgg cttggcgacc
      181 gcgccgacca ggcggaggcg ctggcgctgc ggctcgggct ccgcaccttc gcctcggtca
      241 cgttccccgt cagccccgag gtcgacccct cggccaccat cctggcggca cgtgaagccg
      301 gcgagcggtt cttctgcatg gaacagcccg gccgcgacgg gtacgcggtg tgcggcctcg
      361 gcgaggcgat cgccgtcacc cgctcgggga cggaccggtt cgagggcgcc gcggccgact
      421 gccgccgcat cggcgcccgc acgctggagg acgaccaggc ggccgatccc caccgcccgc
      481 cgggcgcggg gcccgtgttc ctgggcggct tcgcgttcgc ggccaccggc ggcgccaccc
      541 cggagtggtc gggcttcgac ccggcgcagc tggtgctgcc cgaggtctcg atcgcccgca
      601 tgggcgacca ggcccggctg accgtgacgg tggccgtgga cccgcgccag ggtgcggcgg
      661 ccgaggccct ggagcgcgcc cgccgccgga tggcggcgct ggagccggcc acgatgccgc
      721 tgctggatcc cagcccgacc gcccgcccgc gggtggccgg cgccgccgcg ccttcgcact
      781 acgaggacgc cgtccggcgc gcggtcgagc gcatccgctc cggccagatg agcaaggtcg
      841 tgctggcgcg cgaggtgcgc gtgcagaccg gtcgcgagat cgatccagcg cccgtcctgg
      901 acgcgctccg gggcggcttc cccgattgct actgctactg cgtcgggggc gccggcggca
      961 gcttcgtcgg cgccagcccg gagctgctgg tccgccgcga cggcgcgcgg gcgcagacgg
     1021 tggcgctggc cggcaccacc cgccgcagcg ccgaccccgc ggtggacgat cacctcggcg
     1081 agcagctgcg ccagagcccg aagaaccggt ccgagcaggc catcgtcgag cgccggatcc
     1141 agagcacgct ggccccggtc agcgtgtggg tcgcggcggc cgaggagccg gtgctgatca
     1201 aggtgctgaa cgtccagcac ctggccacgc ccatccgggc ccagctctcg gaccccgttc
     1261 cggcggtcga gctggccggc cggctgcacc ccacgcccgc cgtcggcggc gagccccgcg
     1321 ccgtggccga gcccatgatc cccgagctcg aggggctgga ccggggctgg tacgccggcg
     1381 cggtggggtg gaccgacctg tccgaggacg gcgagttctg cgtggccctg cgctgcgcgc
     1441 tgctgcgcgg cgacgtggcc cacctgtacg ccggctgcgg gatcgtggcc gactccgtcc
     1501 ccgccgacga gctggcggag acggagttca agctgcaggc gctgctgccg ctgctggcct
     1561 agcccacggc ggcgcctacg cggcgggcac ggcggtgaag ttcagcgtcc cggcgcagga
     1621 gatgaacgcg atcttggccg ggttggggaa cgcgaactcc agccgcgagt cgctgaacga
     1681 ctggaccatg ctgccgctga tctggtcgcc cgtgcgcgtg gtgttcagcc ggtaggtcac
     1741 ggtccgctcg ccggaggtga ccagcgtgtt gaccctcggc accacctggg tctgggtggc
     1801 gtcctggttg cccagcgccc acggcccgag cttgtcgaac atctccgacg acagcgaggt
     1861 ctgcgcggag ctgtacccac cgctcttgaa gcagctcacc ggctggaacg tcggcgccgc
     1921 cagggtgacc tggccgttct cgacgaccag gtcgaacgtg ccgcccatgc tggtcgtggc
     1981 cttccagcgg ttcgtcgcac cgccgccgct gccgccctgg ccggcgccgg gggcaccgga
     2041 gcccggagcg ccgccggctg cgcccggggc cttgagcacc agcgccttgc ggtagctctt
     2101 ggccttgccg ccgcggcggg ccagccgggc ggtcatggtg gtggccaggc tgcccttgga
     2161 cctcagcgtg gccaggccgg ccttggacag cttcagcgac accttcttgc tgcgcttgcc
     2221 cttgacggac accttgcgtg aggcgagctt ggtcttgccg ctggccaggg cgacggtgcc
     2281 cttcagcgtg tagcggttgg ggttggtcac cttcacggtg gtggccccgg tggcgctgac
     2341 ggcgccgccg gagagcttgg ccggcgcggc cagggccggg ggtgcgaggg cgagcagccc
     2401 gagggccgcg acgacgggga tggaagcggt ggtcttcatg cccccaacct gccgccgatg
     2461 gggccccctg gcatcggggg gatccctaac ccttacccct gacttcccag ggcctcccca
     2521 acacggccgt acagctcgcg gtgcaggcgg acgctgtccg cgcggtcggt gcggacctcg
     2581 gtgaagcggt ccccgagccc cgccagagcc gccagtgcgg ccggc
//
LOCUS       DASUPW010000106         2446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_578228, whole genome shotgun sequence.
ACCESSION   DASUPW010000106 DASUPW010000000
VERSION     DASUPW010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578228"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VFY44_02425"
     CDS             complement(<1..605)
                     /locus_tag="VFY44_02425"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEX5909319.1"
                     /translation="MATAVNLLSDTQTRPSRGMRDAIATADVGDEQKKLDPTTIELQE
                     RVAELLGHEAGLFLPSGSMCNLIAFRLHIRPGGDEAILAANSHPAGFEAGAPAAVNGA
                     MLRLLETPTGIFGPDDVEDALRTPGDRYAPRSRMVSVEQSTNLGGGRVWPLSTVRGVL
                     DVARGAGLRAHMDGARLLNAAVASGVHASAYASGFDTAWLD"
     gene            complement(970..1395)
                     /gene="ribH"
                     /locus_tag="VFY44_02430"
     CDS             complement(970..1395)
                     /gene="ribH"
                     /locus_tag="VFY44_02430"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX5909320.1"
                     /translation="MPDRYAIAVGRFYGDLAERLVDGAQRVFAEAGADVEVHDVPGAF
                     ELPLAAKYLADTGRIAGVACLGAVIRGETSHYDYVCGEAAAGIARVSLDTGVPCAFGV
                     LTVENMDQALARTGDGKRHQGEDAAHAVLRMAELRRQLG"
     gene            complement(1388..>2446)
                     /gene="ribA"
                     /locus_tag="VFY44_02435"
     CDS             complement(1388..>2446)
                     /gene="ribA"
                     /locus_tag="VFY44_02435"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEX5909321.1"
                     /translation="LVMAAQFGTAFTVSIEAREGVSTGISAHDRARTIQVAIDPASRP
                     RDLVQPGHVFPLKAKDGGVLERTGQTEASVDLARLAGLNPSGVICEIMNDDGSMARVP
                     DLEEYCARHELKMVTVADLIAYRRKHDKLIERVVEAAMPTAFGDFNVVGFRSLLDEKH
                     HVAMVKGEVSGKDDVLVRVHSECLTGDVFHSLRCDCGQQLEDALLRIEQEGQGVLLYL
                     AQEGRGIGLLNKLKAYKLQEQGMDTVDANLELGLPADLRDYGIGAQILVDLGLSSLRL
                     LTNNPKKIVGLEGYGLTVTDQVPIEAQPGAHNTDYLRAKAQRMGHLLHHQGLALDEEM
                     ILEEHVTDRERAERDEDA"
BASE COUNT          320 a          884 c          882 g          360 t
ORIGIN      
        1 aagtccagcc aggcggtgtc gaagcccgag gcgtaggccg acgcgtgcac gccgctggcg
       61 acggcggcgt tgagcagccg ggcgccgtcc atgtgcgcgc gcaggccggc gccgcgggcc
      121 acgtccagca cgccgcggac cgtcgagagc ggccacacgc ggcccccgcc gaggttcgtg
      181 ctctgctcca ccgagaccat ccgcgagcgg ggcgcgtagc ggtcgccggg ggtgcgcagg
      241 gcgtcctcga cgtcgtcggg gccgaagatc ccggtcggcg tctccaggag cctgagcatc
      301 gcgccgttca cggccgcggg cgcgccggcc tcgaaccccg ccgggtgcga gttggcggcc
      361 aggatggcct cgtcgccccc gggccggatg tgcaggcgga aggcgatcag gttgcacatc
      421 gagccgctgg gcaggaacag gccggcctcg tggcccagca gctcggccac ccgctcctgc
      481 agctcgatcg tcgtcgggtc gagcttcttc tgctcgtccc caacgtccgc ggtggcgatc
      541 gcgtcgcgca tgccccggct gggccgggtc tgggtgtcgc tgaggaggtt gacggcggtg
      601 gccacgccgg tgaggctagt cggggggcgc gttcgcctgt gatcggcacc agtagatcag
      661 cgacaacggc ggctgggtag ggcggcttct tcgtcaccgt ttgcgctgtt tcgtcacgga
      721 gccccggatg agtccgtaga accgtcgcca tgcgagacgg ttctcgtgac gccgtcgtcc
      781 gaggaccacg gtccgggccg caccgtgacg gcgaggtcac agatgtgacg gcgcggcaac
      841 gaccgacgaa cagacgagcc gcgcggtcga tttctcccgc tcagcgggag caaagcaccg
      901 cgcggtgccc tacccagccg gccgttgcgc cgatccgcgg tgcggccgcg cggcagaacg
      961 cgcgccgcgt cagcccagct gccggcgcag ctcggccatc cgcagcaccg cgtgggccgc
     1021 gtcctcgccc tggtggcgct tgccgtcgcc ggtgcgggcc agcgcctggt ccatgttctc
     1081 gaccgtgagc accccgaagg cgcacggcac gcccgtgtcg agcgagacgc gggcgatgcc
     1141 cgccgccgcc tcgccgcaga cgtagtcgta gtgggaggtc tcgccgcgga tcacggcccc
     1201 gaggcaggcg acgcccgcga tccgcccggt gtcggccagg tacttcgccg ccagcggcag
     1261 ctcgaaggcc ccgggcacgt cgtggacctc cacgtccgcg ccggcctcgg cgaacacgcg
     1321 ctgggcgccg tccaccagcc gctccgccag atcgccgtag aagcggccca cggcgatcgc
     1381 gtagcggtca ggcatcctcg tcccgctccg cgcgctcgcg gtcggtcacg tgctcctcga
     1441 ggatcatctc ctcgtccagg gccaggccct ggtggtgcag caggtggccc atgcgctggg
     1501 ccttggcccg caggtagtcg gtgttgtggg cgccgggctg ggcctcgatc ggcacctggt
     1561 cggtcacggt caggccgtag ccctcgaggc cgacgatctt cttcgggttg ttggtcagca
     1621 ggcgcagcga gctcagcccc aggtccacca ggatctgggc gccgatgccg tagtcgcgca
     1681 ggtcggccgg gagcccgagc tcgaggttgg cgtccacggt gtccatcccc tgctcctgga
     1741 gcttgtaggc cttgagcttg ttcagcaggc cgatgccgcg gccctcctgg gccaggtaca
     1801 gcagcacgcc ctgcccctcc tgctcgatcc gcagcagcgc gtcctccagc tgctgcccgc
     1861 agtcgcagcg cagcgagtgg aacacatcgc cggtcaggca ctccgagtgc acccggacca
     1921 gcacgtcgtc cttgcccgag acctcgccct tgaccatggc cacgtggtgc ttctcgtcca
     1981 gcagcgagcg gaagccgacc acgttgaagt cgccgaacgc ggtgggcatg gccgcctcga
     2041 ccacacgctc gatcagcttg tcgtgcttgc gccggtaggc gatcaggtcc gcgaccgtga
     2101 ccatcttcag ctcgtgccgg gcgcagtact cctcgaggtc gggcacgcgg gccatggacc
     2161 cgtcgtcgtt catgatctcg cagatcacgc cggacgggtt gagccccgcc aggcgggcca
     2221 ggtcgaccga ggcctcggtc tggccggtgc gctcgagcac cccgccgtcc ttggccttga
     2281 gcgggaacac gtggccgggc tggaccaggt cgcgcgggcg cgacgcgggg tcgatggcca
     2341 cctggatcgt gcgcgcgcgg tcgtgcgcgg agatcccggt cgagacgccc tcgcgggcct
     2401 cgatcgacac ggtgaacgcc gtgccgaact gcgccgccat gacgag
//
LOCUS       DASUPW010000107         4240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_994143, whole genome shotgun sequence.
ACCESSION   DASUPW010000107 DASUPW010000000
VERSION     DASUPW010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994143"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..890)
                     /locus_tag="VFY44_02440"
     CDS             complement(138..890)
                     /locus_tag="VFY44_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5909322.1"
                     /translation="MVPIVERSMKEPEWLSNAYLVADEPGGHGVIIDSGGPSEPLLAA
                     IDKHDLTVEHLLLTHHHADHVAENHVYKEKFGVEILAHPLEAQELMDVDRTIEPGDLV
                     LEVGGLKIGALHTPGHTAGMLNFVVNGSEVFTGDTLFKNSVGGVNAPGSTSYADLKSS
                     VMDTLMGLPHETRLHPGHTDPTTVGNEWDANPFIRVWRGLDEEGTEDCVVWERDATLV
                     LNGPDYDGGTKAWIRWKDTGEDDIVPGSQVTS"
     gene            917..1987
                     /locus_tag="VFY44_02445"
     CDS             917..1987
                     /locus_tag="VFY44_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5909323.1"
                     /translation="MDLRSHFPVLERLSYLNAGSVGPVPAEAADAARAELDAQVAEGR
                     GGKPSFDHMIELGDLLRTRVAAVMGCDTGELALTGATTDGVNTVLAGLELEPGDEILT
                     TDEEHPGLLAPLGLAMRRRGVQVRVVPFDAIAAEVRAETRLVACSHVSWLNGKVVDTA
                     GLREAGAPVLLDGAQGIGAIPVDVAELGCDYYAGSGQKWLCGPVGSGYLWVRGDRIEQ
                     LSPAAPGYGSLADPLKALELDLREGAARYDTGLPPSHQSAWALAALDVLEAPGLDAVF
                     ERAAALAERLAGALAAAGHTVAPRGRTTLVSWEAEDPEATVQRLAGEDIGIRNLPGTP
                     YVRASVGAWCTEAELDRLAELA"
     gene            complement(1984..2235)
                     /locus_tag="VFY44_02450"
     CDS             complement(1984..2235)
                     /locus_tag="VFY44_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909324.1"
                     /translation="MRLLADPVVDWGELLNVIWTALLGGIGVTAAFAVALYGAVRATD
                     ARRNGALAVAVGYWTLMALAMALVLASIVFGVVVMTSKD"
     gene            complement(2237..3343)
                     /locus_tag="VFY44_02455"
     CDS             complement(2237..3343)
                     /locus_tag="VFY44_02455"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX5909325.1"
                     /translation="MDAELVLLVIVVVTALAFDFTNGFHDTANVVATSISTRAMAPKI
                     AVGYAAILNFVGAFLSIQVAATIASGIVEADLITEPVVFGGLIGAIAWNLITWYYGLP
                     SSSSHALIGGVVGAALVAEGPDAIIGSGLLGKVAVPAVVAPILAFIVAGVAITVIYRI
                     VGRLRPGPTNAGFKVGQILSGGLLALAHGTNDAQKTMGIITLALIAAGELDAGADPPF
                     WVIVSAATAIALGTYMGGWRIIKTMGSRIIKMDTAQGFAAQGSGAAVILAATHVGFPL
                     STTHTISGGVMGAGAAKRVSAVRWGVAGSIVTAWVLTLPAAAAIGGATYAVTSIFGTG
                     LAGPLIVFVALIITVAVVYARRVAQGSAITAAET"
     gene            complement(3346..3966)
                     /locus_tag="VFY44_02460"
     CDS             complement(3346..3966)
                     /locus_tag="VFY44_02460"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX5909326.1"
                     /translation="MRLSLAPRDREFFDLFEEAGGNILRAADLLDQMLRSYPDRGADL
                     ARDILICEQDGDRITHDIIRRLNETFVTPIDREDIYSLASALDDIVDYTEEVADYLGL
                     YKIEAPMEQSQRLAHILLEAARQISEAIPRLRDFGDISHYTVEVNRLENDGDRVVREA
                     VASLFDNGIDPMVVIRWKDIYERLENAIDSTERAANILEGITIKNS"
     gene            complement(4008..>4240)
                     /locus_tag="VFY44_02465"
     CDS             complement(4008..>4240)
                     /locus_tag="VFY44_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909327.1"
                     /translation="GRHAAGGVRLVNPGSVGMPFDGDPRAAYAVLHDDGRVEHRRVAY
                     DHLASAAAVRGALGPAGQVPAARLERASAQVG"
BASE COUNT          602 a         1504 c         1535 g          599 t
ORIGIN      
        1 gcacgcgggc gagccgcagg tgcgcgactc gctcgagcgc gacggcctgc tcaggcgcta
       61 ggcgggaggc cggcgaagcc ggccgttctc ggggacacgg atgtcccctg gctctcagaa
      121 gcgagggccg gaggccctca agacgtgacc tgggagccgg ggacgatgtc gtcctcgccc
      181 gtgtccttcc accggatcca ggccttcgtg ccgccgtcgt agtcggggcc gttgagcacc
      241 agcgtggcgt cgcgctccca gaccacgcag tcctcggtgc cctcctcgtc aaggccgcgc
      301 cagacgcgga tgaacgggtt ggcgtcccac tcgttgccga ccgtggtcgg gtcggtgtgg
      361 cccgggtgca ggcgcgtctc gtggggcagg cccatgagcg tgtccatgac cgaggacttc
      421 aggtcggcgt agctggtcga gccgggcgcg ttgaccccgc cgaccgagtt cttgaaaagc
      481 gtgtcgccgg tgaacacctc ggagccgttg accacgaagt tgagcatccc ggccgtgtgg
      541 cccggcgtgt gcagcgcgcc gatcttcaac ccgccgacct ccagcaccag gtcaccgggc
      601 tcgatggtgc ggtccacgtc catcagctcc tgcgcctcca gcgggtgggc caggatctcg
      661 acgccgaact tctccttgta cacgtggttc tcggccacgt ggtcggcgtg atggtgggtc
      721 agcagcaggt gctccacggt caggtcgtgc ttgtcgatgg ccgccagcag cggctccgac
      781 gggccgccgg agtcgatgat caccccgtgc cccccgggct cgtcggccac caggtaggcg
      841 ttggacagcc actcgggctc cttcattgag cgctccacga tggggaccat gacccgagcc
      901 tatagtcccg ccaccaatgg acctgcgttc tcatttcccg gtgctcgagc ggctctccta
      961 cctcaacgcc gggtcggtcg gcccggtccc ggccgaggcg gccgacgcgg cgcgcgcgga
     1021 gctggacgcc caggtcgccg agggccgggg cggcaagccc tccttcgacc acatgatcga
     1081 gctcggcgac ctcctgcgca cgcgcgtcgc ggccgtgatg ggctgcgaca ccggcgagct
     1141 ggccctgacc ggcgccacga ccgacggcgt gaacaccgtg ctggccggcc tggagctgga
     1201 gccgggcgac gagatcctga ccaccgacga ggagcacccc ggcctgctgg cgccgctggg
     1261 cctggcgatg cgccgtcgcg gcgtgcaggt gcgggtcgtc ccgttcgacg cgatcgccgc
     1321 cgaggtgcgc gccgagaccc ggctggtggc ctgctcacac gtgtcgtggc tgaacggcaa
     1381 ggtcgtggac accgccggcc tgcgtgaggc cggcgccccg gtgctgctgg acggcgccca
     1441 gggcatcggg gcgatcccgg tggacgtggc cgagctgggc tgcgactact acgcgggatc
     1501 gggccagaag tggctctgcg gccccgtggg cagcggctac ctgtgggtgc gcggcgaccg
     1561 gatcgagcag ctctcccccg ccgcgccggg ttacggctcc ctggccgacc cgctgaaggc
     1621 gctcgagctg gacctgcgcg agggcgcggc ccggtacgac accggtctgc cgcccagcca
     1681 ccaatcggcc tgggcgctgg cggcgctgga cgtgctggag gcccccgggc tggacgccgt
     1741 gttcgagcgc gcggccgcgc tcgccgagcg cctggccgga gccctggccg ccgccgggca
     1801 caccgtcgcc ccgcgtgggc ggaccacgct ggtctcgtgg gaggcggagg atccggaggc
     1861 caccgtccag cgactggcgg gcgaggacat cggcatccgc aaccttcccg gcacgccgta
     1921 cgtgcgcgcc tccgtgggcg cctggtgcac cgaggcggag ctggaccgcc tggccgagct
     1981 ggcctagtcc ttgctggtca tcacgacgac gccgaacacg atgctggcca gcaccagcgc
     2041 catcgccagc gccatcagcg tccagtagcc gaccgccacg gccagcgcgc cgttgcgacg
     2101 cgcgtcggtg gcgcggaccg ccccgtagag cgcgaccgcg aacgcggccg tgaccccgat
     2161 cccgcccagc agggcggtcc agatcacgtt cagcagctcg ccccagtcga cgaccgggtc
     2221 cgccagcagc cgcatctcag gtctccgccg ccgtgatggc cgagccctgc gcgacgcggc
     2281 gcgcgtagac cacggccacg gtgatgatga gcgcgacgaa cacgatcagc ggcccggcca
     2341 gcccggtccc gaagatgctg gtcacggcgt aggtcgcgcc cccgatcgcg gccgccgcgg
     2401 gaagcgtgag cacccaggcg gtcacgatcg acccggccac gccccagcgc acggcggaaa
     2461 cgcgcttggc cgcgccggcg cccatgacgc cgccggagat cgtgtgggtg gtcgacagcg
     2521 ggaagcccac gtgggtggcg gccaggatca ccgcggcgcc gctgccctgg gcggcgaagc
     2581 cctgggcggt gtccatcttg atgatccggc tgcccatggt cttgatgatc cgccagccgc
     2641 ccatgtaggt gcccagggcg atcgcggtcg cggccgagac gatgacccag aacggcgggt
     2701 cggcgccggc gtccagctcg ccggccgcga tcagggccag cgtgatgatg cccatcgtct
     2761 tctgcgcgtc gttggtgccg tgcgccagcg ccaggagccc gccggacagg atctgcccga
     2821 ccttgaagcc cgcgttggtc gggccgggcc ggagccggcc gacgatccgg tagatcacag
     2881 tgatggccac cccggccacg atgaaggcca ggatcggcgc gaccaccgcc ggcacggcca
     2941 ccttgcccag cagcccagag ccgatgatgg cgtcgggccc ctcggccacc agcgcggcgc
     3001 ccacgacgcc gccgatgagc gcgtgcgacg agctggacgg cagcccgtag taccaggtga
     3061 tcaggttcca cgcgatcgcc ccgatcaggc ccccgaagac caccggctcg gtgatcaggt
     3121 cggcctcgac gatgccgctg gcgatggtgg ccgcgacctg gatcgacagg aacgcgccga
     3181 cgaagttcag gatcgccgcg tagcccaccg cgatcttcgg cgccatggcg cgcgtggaga
     3241 tcgaggtggc caccacgttg gcggtgtcgt ggaagccgtt ggtgaagtcg aaggccagcg
     3301 ccgtgaccac cacgatgacc agcagaacga gctcggcgtc cacggtcagg agttcttgat
     3361 ggtgatgccc tcgagaatgt tggccgcgcg ctcggtggag tcgatggcgt tctcgagccg
     3421 ctcgtagatg tccttccagc ggatcacgac catcgggtcg atgccgttgt cgaacagcga
     3481 ggccaccgcc tcgcggacca cgcggtcgcc gtcgttctcc agccggttga cctcgaccgt
     3541 gtagtgcgag atgtcaccga agtcgcgcag gcgcgggatg gcctcggaga tctggcgcgc
     3601 cgcctcgagc aggatgtgcg ccaagcgctg ggactgctcc atcggcgcct cgatcttgta
     3661 cagcccgagg tagtccgcga cctcctcggt gtagtcgacg atgtcgtcca gcgccgaggc
     3721 cagcgagtag atgtcctcgc ggtcgatcgg ggtgacgaag gtctcgttca ggcgccggat
     3781 gatgtcgtgc gtgatccggt ccccgtcctg ctcgcagatg aggatgtcgc gcgccaggtc
     3841 ggccccgcgg tccgggtacg agcgcagcat ctgatcgagg agatcagcag cacggaggat
     3901 gttcccgcca gcttcttcga acaggtcgaa gaactcgcgg tcgcgggggg caagcgacag
     3961 gcgcatggcg cggccacgct agcagttgta aaagccggaa cccggcttca gccgacctgg
     4021 gcgctcgccc gctccagccg cgccgctggc acttgtccgg cgggcccgag cgcgccacgc
     4081 acggcggcgg cgctggccag gtggtcgtag gccacgcgcc ggtgctcgac gcggccgtcg
     4141 tcgtgcagca ccgcgtacgc ggcgcggggg tcgccgtcga agggcatgcc gacgctgccc
     4201 gggttgacga ggcgcacgcc gcccgcggca tgtcgcccga
//
LOCUS       DASUPW010000108         3321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_855924, whole genome shotgun sequence.
ACCESSION   DASUPW010000108 DASUPW010000000
VERSION     DASUPW010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855924"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VFY44_02470"
     CDS             <1..726
                     /locus_tag="VFY44_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909328.1"
                     /translation="ISPQSQPSHGYVIYDETVRGAAENRLAMTIAQLDEAGIKATGAV
                     MDPDPYSAIMDALGERDFDEIVISTHPSTRSGWLRQDLVQRVEQAARRPVEHVVVDLD
                     ADKDSATRTLVVANQTVGGQALIDQLKALNEEGPRRFVVISPQSGEGDEQAADRLART
                     LERLESEGIKAIGQVTHPDPYTAIHNALDFYGIDEVVISTFSEMQSGWLRSDLVGRVR
                     NSTSKPVHHVVSDDAPNAQEASA"
     gene            726..1352
                     /locus_tag="VFY44_02475"
     CDS             726..1352
                     /locus_tag="VFY44_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX5909329.1"
                     /translation="MESASVALGRHVDGDEHDHHGPPVANQSSRVDATTLGMLLFIIS
                     EIMLFGAFFTAYFFIRVVNGDGWPAEGMELPKVIAGVNTAILISSSFTMHWALEGARN
                     ENRRALKAGMLTTMLLGLTFLCIQINEYVHVGFAIGENAQSSIFYGLTGLHGAHVFVG
                     LTLLAFATIRSFRGHFSSKEHMGVEIPGIYWHFVDVMWIVVYTTVYII"
     gene            1484..2221
                     /locus_tag="VFY44_02480"
     CDS             1484..2221
                     /locus_tag="VFY44_02480"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX5909330.1"
                     /translation="MALRAATAAGELLMSRYEGPASGLEQKSSRTDMVSDADKDAEAL
                     VRDLLAAERPDDGLLAEEGSHSDATSGRRWVVDPLDGTTNYLYRFPAWNVSVALEDEQ
                     GSLVGVVHDPLRGETFTAVRGEGSFLGDRRLAMTPPSGLDTALVATGFGYSSERRATQ
                     AQLLTRVLPRVRDIRRAGAAALDLCWVAAGRVDAYYERGLNHWDWAAASLVVSEAGGE
                     LHPLEGEPAGLVAGPPGIAGELHALVA"
     gene            complement(2476..3144)
                     /locus_tag="VFY44_02485"
     CDS             complement(2476..3144)
                     /locus_tag="VFY44_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX5909331.1"
                     /translation="MTAGASGCGRSGDDDDIAGKTAFIEKCGSCHTLARANTKGVQGP
                     NLDEAFGPARASGLGAATVEGVVYDQISHVRRNSTMPKDLVKGKTAHDVAAYVAAVAG
                     QKGKDEGLLATAGPKASNKLIPAKGGTLLMPADPNGGLFYASTRAAAKAGSLKVESKN
                     ESSTGHDIALKDASGKELGGGKVVQGGGVSEFTASVKPGKYEYLCTVPGHAEGGMKGT
                     LTVK"
BASE COUNT          472 a         1221 c         1128 g          500 t
ORIGIN      
        1 atctcgcccc agagccagcc cagccacggc tacgtgatct acgacgagac cgtccgcggc
       61 gccgccgaga accggctggc catgaccatc gcccagctgg acgaggccgg catcaaggcc
      121 accggcgcgg tcatggaccc cgacccctac tcggcgatca tggacgcgct cggcgagcgc
      181 gacttcgacg agatcgtgat ctccacccac ccctccaccc gctcgggctg gctgcgccag
      241 gacctggtcc agcgcgtcga gcaggccgcc cggcggcccg tcgagcacgt ggtggtcgac
      301 ctggacgccg acaaggacag cgccacccgc acgctcgtcg tggccaacca gaccgtgggc
      361 ggccaggcgc tgatcgacca gctcaaggcc ctgaacgagg agggcccgcg ccgcttcgtg
      421 gtcatctcgc cccagtcggg cgagggcgac gagcaggccg ccgaccggct ggcgcgcacg
      481 ctcgagcggc tggagtcgga gggcatcaag gccatcggcc aggtgaccca ccccgacccg
      541 tacaccgcga tccacaacgc gctggacttc tacggcatcg acgaggtcgt gatctccacc
      601 ttctccgaga tgcagtcggg ctggctgcgc tccgacctgg tcggccgcgt ccgcaactcg
      661 acctccaagc ccgtgcacca cgtcgtgtcc gacgacgccc ccaacgccca ggaggccagc
      721 gcctgatgga atccgcctcc gtcgcactcg gccgtcacgt ggacggggac gagcacgatc
      781 accacggccc cccggtggcc aaccagagct cccgcgtgga cgccaccacg ctcgggatgc
      841 tgctgttcat catctccgag atcatgctct tcggagcgtt cttcacggcc tacttcttca
      901 tccgggtcgt gaacggcgac gggtggccgg ccgaggggat ggagctgccc aaggtcatcg
      961 ccggcgtgaa caccgccatc ctgatctcgt ccagcttcac gatgcactgg gcgctggaag
     1021 gcgcgcgcaa cgagaaccgc cgcgcgctca aggccggcat gctcaccacg atgctgctgg
     1081 gcctgacgtt cctgtgcatc cagatcaacg agtacgtgca cgtgggcttc gccatcggcg
     1141 agaacgccca gtcgtcgatc ttctacggcc tgaccggcct ccacggcgcg cacgtgttcg
     1201 tgggcctgac gctgctggcg ttcgccacca tccgttcctt ccggggccat ttctcgagca
     1261 aggagcacat gggcgtcgag atccccggga tctactggca cttcgtggac gtcatgtgga
     1321 tcgtggtcta cacgaccgtc tacatcatct agcgcgtgct gaacccgttc cgctcggagc
     1381 aggacgcgtt ccgggtgttc ctctacgtgg gcggcttctt catcgcgctc gccatcgccg
     1441 tcgtcatcgt gcgggcgctc tgagcgcccg ggacgacctc gacctggccc tccgcgccgc
     1501 gaccgcggcc ggcgagctgc tgatgtcgcg ctacgagggg ccggccagcg gcctggagca
     1561 gaagtcctcg cgcacggaca tggtctccga cgccgacaag gacgccgagg ccctggtgcg
     1621 cgacctgctg gcggcggagc gccccgacga cggcctgctg gccgaggagg gctcgcacag
     1681 cgacgccacc agcgggcggc gctgggtcgt ggaccccctg gacggcacca ccaactacct
     1741 ctaccgcttc cccgcctgga acgtcagcgt ggcgctggag gacgagcagg gcagcctggt
     1801 cggggtggtg cacgacccgc tgcggggcga gaccttcacc gccgtccgcg gcgaggggtc
     1861 gttcctcggc gaccgccgcc tggccatgac gccgcccagc ggcctggaca ccgccctggt
     1921 ggccaccggc ttcggctatt cgtccgagcg ccgcgccacc caggcccagc tgctgacccg
     1981 ggtgctgccg cgcgtgcgcg acatccgccg cgccggggcg gcggcgttgg acctgtgctg
     2041 ggtggccgcg ggccgcgtgg acgcctacta cgagcgcggc ctgaaccact gggactgggc
     2101 cgccgcgagc ctggtggtct ccgaggcggg gggcgagctg caccccctcg agggcgaacc
     2161 ggccggcctg gtggccgggc cgccggggat cgccggcgag ctgcacgcgc tggtggcctg
     2221 agccccggcg cgctgcgctt tcccctgttg ccagcgcggt gactggatcc gcactccgac
     2281 ccggccaggc gcccttcgta agcacggttc atgcgtttgg gtcgctataa cgtccgaaat
     2341 cgacatgccg tggaggccgc ctcccgcgga gtcacccgag cgctacgaac gcaacggaaa
     2401 cgtcacagga cccccgccct acccagccca gccgttcgcc gatccccgaa gggaccgatc
     2461 gaacccggcg cggccctact tgacggtcag cgtgcccttc atgccgccct cggcgtgacc
     2521 gggcacggtg cagaggtact cgtacttgcc gggcttgacg ctggcggtga actccgagac
     2581 gcctccgccc tggaccacct tgccgccgcc gagctccttg ccggaggcat ccttgagcgc
     2641 gatgtcgtgc ccggtcgagg actcgttctt gctctcgacc ttcagcgacc cggccttcgc
     2701 ggccgcgcgc gtggaggcgt agaacagtcc accgttggga tcggcgggca tcaggagggt
     2761 cccgcccttg gcggggatca gcttgttcga ggccttgggg ccggcggtgg ccagcaggcc
     2821 ctcgtccttg cccttctgcc cggccacggc ggccacgtag gcggcgacgt cgtgggccgt
     2881 cttgcccttg accaggtcct tgggcatggt gctgttccgg cgcacgtggg agatctggtc
     2941 gtagacgacg ccctcgacgg tggcggcccc caggccggag gcgcgggcgg ggccgaaggc
     3001 ctcgtccagg ttggggccct ggacgccctt ggtgttggcg cgggccagcg tgtggcacga
     3061 gccgcacttc tcgatgaagg ccgtcttgcc ggcgatgtcg tcgtcgtcac ccgagcggcc
     3121 gcagccgctg gcgcctgccg tcaccagcag ggcgccgcag gcaatcaggg ccagacgctg
     3181 cgaagcactc ctcacgaggc ggaacattat ctgttcaggc ggctgggctg acgagctcgt
     3241 ccaaccggcc ctcggccatg gcgatcacgc ccttggccac cgcctcccag ccgaagcgct
     3301 cgcgggccgt gcgggccagc g
//
LOCUS       DASUPW010000109         2937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_856542, whole genome shotgun sequence.
ACCESSION   DASUPW010000109 DASUPW010000000
VERSION     DASUPW010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856542"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..757
                     /locus_tag="VFY44_02490"
     CDS             98..757
                     /locus_tag="VFY44_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909332.1"
                     /translation="MSAAPLRRRAPVRHPGAPARRPHAAPRRPVPGVRAVRPAGQAVL
                     DRLLSGRLWIGLVFLLLVGIVFFNVDLLRMNRDIAATAEKAETIKRQNARLRGDLARL
                     GSSERIQTAAAQRGLVLPAAGEVRYLKANPGADAKNALARLKAGGGAAATEEGVPPAQ
                     AEPGETAQEQTATATPPATTTPQTTTPQTATPAPTATPQSATPQAPVAAVPQPTTQTG
                     Q"
     gene            761..2413
                     /locus_tag="VFY44_02495"
     CDS             761..2413
                     /locus_tag="VFY44_02495"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX5909333.1"
                     /translation="MRSTLLDRRIGLLFAVFLLLLGLATLRATWLGTVKADSLSERAA
                     TQQVEELKVPAGRGTITDRKGVELAVSEEATTVFANPMLIKNPSKVAAELAPLLGRDQ
                     SELLPLLSDKDKGFVYLRRKLDPTLGDKISRLRIEGIDTVSEPNRTYPQGALAGQLLG
                     SVGLDGNGLAGIEQQFDQELHGEDGQRKLVKDATGKVISLADTKRQESGEDLKLTIDA
                     ALQERVEAVLGQVARDHRPKGATALVMDPRNGELLAMANYPRVNPNDLINSPKDGLQN
                     RAVGSAFEPGSTFKAFTVAGALQDGKVTPETELDLPSQLQVADRTIGEAHDRGAVTLT
                     TAQILAQSSNVGSVMIGQKLGPARFDHWVRKFGFGKPTGVSLPGEGGGIVPAPKDYSG
                     SSMGNLPIGQGLAVTPLQMAAGFSALANGGVLHRPHVVAGQSDPGKRVVAPKTAAQVA
                     RMLEGVLAAGGTAQEASIPGYKLAGKTGTAQKPDPENGGYSEFKFFSSFIGFAPAENP
                     RLMVAVMVDEPTNGYYGGEVAAPAFEKIVSFALPYMKIPPSG"
     gene            2410..>2937
                     /locus_tag="VFY44_02500"
     CDS             2410..>2937
                     /locus_tag="VFY44_02500"
                     /inference="COORDINATES: protein motif:HMM:NF018525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5909334.1"
                     /translation="MSAPSSVLLLHGWQNRREADHWQSRVAAMLRERDTEVAHPQLPD
                     PDEPDPDAWAATARAALDEMTPGHRVVVCHSLGCLLWARIAEPVDRVCWVAPPGPDEL
                     RAYTAITAFLPDRLDAEALRASSTTAPLRLVCSDDDPYCAAGAGAAYGEPLGLDTEVL
                     PGQAHINPDSGYGDWP"
BASE COUNT          421 a         1144 c         1023 g          349 t
ORIGIN      
        1 cggtggcgcc cacgcccggc gaggtggccg ccaacccgcg ctcggggtcg gcgcgcctgc
       61 gcgccgcccg ccggatcggc gacggggtgg agcgctgatg tcggccgctc cactgcgccg
      121 ccgcgcgccc gtccgccatc ccggcgcccc cgctcgtcgc ccgcatgccg ctccccgccg
      181 cccggtcccc ggcgtccgcg cggttcgccc cgcgggccag gcggtcctgg accggctgct
      241 ctcgggacgg ctgtggatcg gcctggtgtt cctgctgctg gtggggatcg tgttcttcaa
      301 cgtcgacctg ctgcggatga accgcgacat cgcggccacc gccgagaagg ccgagaccat
      361 caagcgccag aacgcccgcc tgcgcggcga cctggcgcgc ctgggctcca gcgagcggat
      421 ccagaccgct gccgcccagc gcggcctggt gctgcccgcc gccggcgagg tccgctacct
      481 caaggccaac cccggcgccg atgccaagaa cgccctggcc cgcctgaagg ccggcggtgg
      541 cgccgccgcg accgaggaag gggtcccgcc cgcccaggcc gagcccggcg agacggcgca
      601 ggagcagacg gccacggcga cgccacccgc gaccaccacg ccgcagacca ccactccgca
      661 gaccgcgacg cccgcgccca cggcgacgcc gcagagcgcc acgccccagg cgcccgtcgc
      721 cgccgtgcca cagccgacga cccagacggg ccagtagcgc gtgcggtcga cccttctcga
      781 ccgcaggatc ggactcctct tcgccgtctt cctcctcctc ctggggctgg cgacgctgcg
      841 cgccacctgg ctgggcacgg tcaaggccga ttcgctgtcc gagcgcgccg ccacccagca
      901 ggtcgaggag ctgaaggtgc ccgccggccg cggcaccatc accgaccgca agggggtcga
      961 gctggcggtc tccgaggagg cgacgacggt gttcgccaac ccgatgctca tcaagaaccc
     1021 gtccaaggtg gcggccgagc tggcgccgct gctgggccgc gaccagagcg agctgctgcc
     1081 gctgctgtcc gacaaggaca agggcttcgt gtacctgcgc cgcaagctcg acccgacgct
     1141 gggcgacaag atctcgcggc tgcggatcga gggcatcgac accgtctccg agcccaaccg
     1201 gacctacccc cagggcgccc tggccggcca gctgctgggc tcggtgggcc tggacggcaa
     1261 cggcctggcc ggcatcgagc agcagttcga ccaggagctg cacggcgagg acggccagcg
     1321 caagctggtc aaggacgcca ccggcaaggt catcagcctg gccgacacca agcgccagga
     1381 gtccggcgag gacctgaagc tgaccatcga cgccgcgctc caggagcggg tcgaggcggt
     1441 gctgggccag gtggcccgcg accaccgccc gaagggtgcc accgcgctgg tcatggaccc
     1501 gcgcaacggc gagctgctgg ccatggccaa ctacccgcgc gtgaacccca acgacctgat
     1561 caactcgccc aaggacggcc tccagaaccg tgccgtgggc tcggccttcg agcccggctc
     1621 cacgttcaag gccttcaccg tggccggcgc gctgcaggac ggcaaggtca cgcccgagac
     1681 cgagctcgac ctgccgtcgc aactccaggt ggccgaccgc accatcgggg aggcgcacga
     1741 ccgcggcgcg gtgaccctga cgacggccca gatcctggcc cagtcgtcca acgtggggtc
     1801 ggtcatgatc ggccagaagc tcggccccgc gcgcttcgac cactgggtgc gcaagttcgg
     1861 cttcggcaag cccacgggcg tctccctccc cggcgagggc ggcggcatcg tgcccgcgcc
     1921 caaggactac tccggctcgt cgatgggcaa cctgccgatc ggccagggcc tggccgtgac
     1981 gccgctccag atggccgcgg gcttctcggc gctggccaac ggcggcgtgc tgcaccgccc
     2041 gcacgtcgtg gcgggccaga gcgaccccgg caagcgcgtg gtggcgccca agaccgccgc
     2101 ccaggtggcg cggatgctcg agggcgtgct ggccgccggc ggaaccgccc aggaggcctc
     2161 gatccccggc tacaagctgg ccggcaagac cggcacggcc cagaagcccg atcccgagaa
     2221 cggcggctac tccgagttca agttcttctc ctcgttcatc ggcttcgccc ccgccgagaa
     2281 cccgcgcctg atggtggccg tgatggtcga cgagcccaca aacggctact acggcggcga
     2341 ggtggccgcg ccggccttcg agaagatcgt ctccttcgcg ctcccgtaca tgaagatccc
     2401 gccgagcgga tgagcgcgcc ctcctccgtt ctgctgctgc acggatggca gaaccggcgc
     2461 gaggccgacc actggcagag ccgcgtagcg gccatgctgc gcgagcggga caccgaggtc
     2521 gcgcacccgc agctgcccga ccccgatgag cccgatccgg acgcctgggc agccaccgcg
     2581 cgcgccgcgc tggatgagat gacgcccggc caccgggtgg tggtgtgcca ctcgctgggg
     2641 tgcctgctgt gggcccggat cgccgagccc gtcgaccgag tgtgctgggt ggcgccgccc
     2701 ggcccggacg agctgcgtgc ctacaccgcg atcaccgcgt tcctgcccga ccggctcgac
     2761 gccgaggcgc tgcgggcctc gtccacgacc gccccgctgc ggttggtgtg ctcggacgac
     2821 gacccctact gcgccgcggg cgccggcgcg gcctacggcg agccgctggg cctggacacc
     2881 gaggtcctgc ccggccaggc gcacatcaac cccgactcgg gctacggcga ctggccg
//
LOCUS       DASUPW010000110         2529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_994917, whole genome shotgun sequence.
ACCESSION   DASUPW010000110 DASUPW010000000
VERSION     DASUPW010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994917"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VFY44_02505"
     CDS             complement(<1..786)
                     /locus_tag="VFY44_02505"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5909335.1"
                     /translation="MPADQTLRVAICGGGMRSRRVWQRHVAQEPGFELVGVMDPSTEA
                     LDASREEGHVPADGLFTELDGMLDATGPDVLLACPIIEAHGAAVRAGLEAGCHVLVEK
                     PFVTDVVEATELTELAERRDLTLGVVQNWRTRSNSQALKRALDEDRIGPVSHIFFRYV
                     RDREKPHLPDYLFDEADPILWAMGIHHLDLFRYLLGQDIVRVHGESARPAWSRYRVPS
                     INQLWMETEGGVVISYVATFSSRNAHVPQESLQIEGEKGTLYND"
     gene            complement(776..1711)
                     /locus_tag="VFY44_02510"
     CDS             complement(776..1711)
                     /locus_tag="VFY44_02510"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5909336.1"
                     /translation="MSDGRILVTGGAGFIGSHLARALLAEGHTVDVADDLSTGAAADV
                     PGGADLIEVDLGARGAVGPALEGRSYSAICHLAGQSSGEKSFDDPGHDLDANARSTVA
                     LAAWALEHDVPVLVHASSMGVYGQPDRSPVAESAPTLPLSWYGASKLAAERALAVAAG
                     GGLRTASMRMFSIYGPGQDLSEMRQGVVSIFMAMALRDQPIDVHGTLDRVRDFVYVDD
                     CVEAWVRTLRGDVSGPLNVGTGVGTSFRDMVGEMLAQMGRPDHPVHERGTPTPGDQRA
                     LYADTARMREQLGWAPQTPLGDGLRAMLGWAADAR"
     gene            complement(1708..>2529)
                     /locus_tag="VFY44_02515"
     CDS             complement(1708..>2529)
                     /locus_tag="VFY44_02515"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5909337.1"
                     /translation="TPAHTDGWRGLWLHSYRLGLRWLVRAAPRWRGAKVGILRLLVPM
                     DPWRYYELGRVAEQPFAGPCLDVSSPKLLTSWLQRQGKGQWTGIDLFTAEIEGWKQVD
                     PDLRLEVQDATGLPYPDASFDHCICISVIEHVPGEGDSAAMAEMWRVLRPGGVLELTT
                     EVARSGGDRYVDDHLYGEASPGEEGRVFFGRRYTDAEVGERLLREPWEELEREWARWR
                     DPALEETFYRRAPWSYLYGGLLRLRAPGNVEVGDTIDILGPDEPGALYLRLRKPA"
BASE COUNT          341 a          940 c          916 g          332 t
ORIGIN      
        1 gtcgttgtac agcgtcccct tctcgccctc gatctgcagc gactcctgcg gcacgtgggc
       61 gttgcgcgac gagaacgtgg ccacgtagga gatgaccacg ccgccctcgg tctccatcca
      121 cagctggttg atcgagggca cccggtagcg gctccaggcg ggccgcgccg actcgccgtg
      181 cacgcgcacg atgtcctggc ccagcaggta gcggaacagg tccaggtggt ggatgcccat
      241 ggcccacagg atcgggtcgg cctcgtcgaa caggtagtcc gggaggtgcg gcttctcgcg
      301 gtcgcggacg taacggaaga agatgtgcga gaccggtccg atgcggtcct cgtcgagggc
      361 ccgcttgagc gcctgggagt tggagcgggt gcgccagttc tgcacgacgc ccagcgtcag
      421 gtcccggcgc tcggccagct cggtgagctc ggtcgcctcg acgacgtcgg tcacgaacgg
      481 cttctcgacc agcacgtggc acccggcctc gagcccggcg cgcacggcgg cgccgtgcgc
      541 ctcgatgatc gggcacgcca gcagcacgtc cgggccggtg gcgtccagca tcccgtccag
      601 ctcggtgaac aggccgtcgg ccggcacgtg gccctcctcg cgggaggcgt ccagcgcctc
      661 ggtcgagggg tccatgaccc ccaccagctc gaagccgggc tcctgggcca cgtggcgctg
      721 ccacacgcgg cgcgagcgca tgccgccgcc gcagatggcc acgcgcaggg tctggtcagc
      781 gggcatcggc ggcccaacct agcatcgccc ggaggccgtc cccgagcggt gtctgcggcg
      841 cccagccaag ctgctcgcgc atccgcgcgg tgtcggcgta cagggcccgc tggtcgccgg
      901 gggtcggcgt gccgcgctcg tggacggggt ggtcgggccg gcccatctgg gccagcatct
      961 cgccgaccat gtccctgaac gaggtgccca cgccggtgcc cacgttcagc gggccgctca
     1021 cgtccccgcg cagcgtgcgc acccaggcct cgacgcagtc gtccacgtag acgaagtccc
     1081 gcacccggtc gagggtgccg tggacgtcga tgggctggtc gcgcagggcc atggccatga
     1141 agatcgacac caccccctgg cgcatctcgc tcaggtcctg gcccgggccg tagatcgaga
     1201 acatgcgcat cgaggcggtg cgcagcccgc cgcccgcggc caccgccagc gcgcgctcgg
     1261 cggccagctt cgaggcgccg taccaggaca gcggcagcgt gggcgcggac tccgccaccg
     1321 gcgagcggtc gggctggccg tagacgccca tcgacgaggc gtgcaccaac accggcacgt
     1381 cgtgctccag cgcccacgcc gccagcgcca cggtcgagcg cgcgttggcg tccaggtcgt
     1441 gcccggggtc gtcgaaggac ttctcgcccg acgactgccc ggccaggtgg cagatggccg
     1501 agtaggagcg tccctcgagc gccggcccca ccgcaccccg ggcgcccagg tcgacctcga
     1561 tcaggtcggc gcctccgggc acgtcggcgg cggcgccggt ggagaggtcg tcggccacgt
     1621 ccacggtgtg cccctcggcc agcagcgcgc gggccaggtg cgagccgatg aagcccgcgc
     1681 cgccggtgac caggatccgg ccgtcgctca tgccggcttg cgcagccgca ggtagagggc
     1741 gccgggctcg tcgggcccca ggatgtcgat cgtgtcgccc acctccacgt tgcccggcgc
     1801 ccgcaggcgc agcagcccgc cgtagaggta cgaccacggc gcgcgccggt agaaggtctc
     1861 ctccagcgcg gggtcgcgcc agcgcgccca ctcgcgctcg agctcctccc acggctcccg
     1921 cagcagccgc tcgcccacct cggcgtcggt gtagcggcgg ccgaagaaca cgcgcccctc
     1981 ctcgcccggg ctggcctccc cgtacaggtg gtcgtccacg tagcggtcgc caccgctccg
     2041 ggcgacctcg gtggtcagct cgagcacgcc gccgggacgg agcacgcgcc acatctcggc
     2101 catcgcggcg ctgtcgccct caccgggcac gtgctcgatc accgagatgc agatgcagtg
     2161 gtcgaagctg gcgtcggggt agggcaggcc ggtggcgtcc tggacctcca ggcgcaggtc
     2221 ggggtcgacc tgcttccagc cctcgatctc ggcggtgaac aggtcgatcc ccgtccactg
     2281 gcctttgccc tgacgctgga gccagctggt cagcagcttg gggctggaca cgtccaggca
     2341 cgggccggcg aacggctgct cggccactcg gccgagctcg tagtagcgcc acggatccat
     2401 cgggacgagc agccggagga tgcccacctt cgcgccgcgc cagcgtgggg cggcgcgcac
     2461 cagccagcgc agccccaggc ggtaggagtg gagccagagg ccgcgccagc cgtcggtgtg
     2521 ggcgggggt
//
LOCUS       DASUPW010000111         3687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_995019, whole genome shotgun sequence.
ACCESSION   DASUPW010000111 DASUPW010000000
VERSION     DASUPW010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995019"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VFY44_02520"
     CDS             complement(<1..807)
                     /locus_tag="VFY44_02520"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5909338.1"
                     /translation="MSARKARRAMAVASLIVAIAAIAIVLTRSDGATYEVRATFDDVR
                     GLIEGGDVRAGAVPVGKVTKIEIGPGERPLVTMAIDDDFRLRQGATADIRLASNVGAV
                     NRTVELEQGDEGAPRLRDGARLRGGQTDQPVNFDEVMDSLTPRARRDIANLLGGLEAG
                     TRDRGPDIDRTLRHSALALGNTAALLAEVNQDGQALRTIVGDGERVVSSLASRPGDLG
                     ATAERLAGLLDATAARRAELGESVRLLGPALADGRRTLDAVAAATPELRRL"
     gene            complement(804..1628)
                     /locus_tag="VFY44_02525"
     CDS             complement(804..1628)
                     /locus_tag="VFY44_02525"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5909339.1"
                     /translation="MKLRDRPPSRVRRAIEAPDYVPVIDPLGTFTSFVLSVLGAFRLL
                     PRYSGEVVRQVGMLAAGSVLVIVLMSFVAGTSCGIAAEAFGQQIGAGVVGPLFSSFCV
                     NREIVPFVVGFIVAAKVGGGIVAELGAMRVNEEVDAMDVMGVPSIAFAVACRLAAVFI
                     VLPIAYLVALGAAEGAAWIGSYVRSGSVSQGTWEFAFYTVTGVTDIFLSFVKGLVMTI
                     AVVLVALYYGYHVSGGPVEVGTATARSMAVNLILVTVLNMVMTFLFWGFNPRLPIA"
     gene            complement(1625..2440)
                     /locus_tag="VFY44_02530"
     CDS             complement(1625..2440)
                     /locus_tag="VFY44_02530"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5909340.1"
                     /translation="MLTPWAPFARADVPPGLWVANAGKTVGLGMRTFAQLVSPGLSWR
                     NELLRQFVFAFRLTLAPASIVAFVVGFGTIGIMGGSLASAFGAVDRVAPVAPLVFLRE
                     LGPLLTTSIVAGTLGATITAEIAARTIRDELTALEVLGINPVRNLVLPRVVALTLWMP
                     VLALLTFWAGIAGTFTATVLLYDATPQAFAGQLLGLTNFIDLWGSVVKLALFGALVGI
                     IAAQKGLQVEGGAEGVGRAVNESVVTCFVAIGIVTVAYTQLFQAFFPEVNFGG"
     gene            2579..3643
                     /locus_tag="VFY44_02535"
     CDS             2579..3643
                     /locus_tag="VFY44_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX5909341.1"
                     /translation="MTAAAVLREQGGAFALEEVEVDAPRAGEVLVRVAGVGICHTDLA
                     AADGALGLPLPIVLGHEGAGTVEAVGPGVTAVAPGDAVVLTYDHCGACTRCTAGHPAY
                     CERFAALNASGARADGTTTLSGTGGPVHGSFCGQSSFAALALAGERNAIPVSTDAPLE
                     LLGPLGCSVQTGAGAVLEVLGAQPGQGIAVVGLGAVGLSAVMAAVVAGCDPIVAIDPD
                     RCRRELAEELGATHALDGERRPRAGVDLSVDAVGTQDALTAALRALAAPGHCATVGWR
                     GPRNPLTVDQGHLLQGRSLTGVIEGDTVPGRFIPHLLELHAAGRFPFERLVQTVPFDR
                     IGEAVEAARAGRVVKPVLVL"
BASE COUNT          525 a         1395 c         1324 g          443 t
ORIGIN      
        1 cagcctgcgc agctccggcg tcgccgccgc gaccgcgtcc agcgtccggc ggccgtcggc
       61 cagcgccggg cccagcaggc gaacggactc gccgagctcc gcccgccggg ccgcggtcgc
      121 gtccagcagc cccgcgagcc gctcggccgt cgcacccagg tcccccggcc gcgaggccag
      181 gctcgagacc acccgctcgc cgtcgcccac gatcgtccgc agcgcctggc cgtcctggtt
      241 gacctccgcc aggagcgcgg cggtgttgcc cagggcgagg gcgctgtggc gcagggtccg
      301 gtcgatgtcc ggcccgcggt cgcgggtgcc cgcctccagg ccgccgagca ggttcgcgat
      361 gtcccgccgg gcgcgcggcg tgagcgagtc catcacctcg tcgaagttca cgggctgatc
      421 ggtctggccg ccgcgcagcc gcgcgccgtc ccggagccgc ggcgcgccct cgtcgccctg
      481 ctcgagctcg acggtgcggt tgaccgcgcc cacgttcgag gccagccgga tgtcggccgt
      541 ggccccctgg cggaggcgga agtcgtcgtc gatggccatc gtgaccagcg gtcgctcgcc
      601 gggcccgatc tcgatcttgg tcaccttgcc caccggcacc gccccggcgc ggacgtcgcc
      661 gccctcgatc aggccgcgga cgtcgtcgaa ggtggcccgg acctcgtagg tggcgccgtc
      721 ggagcgggtc aggacgatcg cgatcgccgc gatcgcgacg atcagcgaag cgaccgccat
      781 ggcccttctc gccttgcggg cgctcacgcg atcggcagcc tcgggttgaa gccccagaac
      841 aggaacgtca tgaccatgtt caggaccgtg accaggatca ggttgaccgc catcgaccgg
      901 gccgtcgcgg tgcccacctc caccggccca ccgctgacgt ggtagccgta gtacagggcc
      961 acgagcacga cggcgatcgt catgaccagc cccttcacga acgacaggaa gatgtcggtc
     1021 acgccggtga ccgtgtagaa ggcgaactcc caggtgccct gcgacacgct cccggagcgg
     1081 acgtacgagc cgatccaggc cgcgccctcc gcggcgccga gcgccacgag gtaggcgatc
     1141 ggcaggacga tgaacaccgc cgccagccgg caggccaccg cgaacgcgat cgacgggacg
     1201 cccatcacgt ccatcgcgtc gacctcttcg ttcacgcgca tcgcgcccag ctcggccacg
     1261 atgccgcccc ccaccttcgc cgcgacgatg aagccgacga cgaagggcac gatctcgcgg
     1321 ttcacgcaga aggagctgaa cagcgggccg accacgccgg ccccgatctg ctgaccgaac
     1381 gcctcggccg cgatgccgca gctggtgccc gccacgaacg acatcagcac gatcaccagc
     1441 acgctcccgg ccgcgagcat cccgacctgg cgcacgacct cacccgagta ccgcggcagc
     1501 agccggaacg cgcccaggac gctgagcacg aacgaggtga acgtgccgag ggggtcgatg
     1561 accggcacgt agtccggcgc ctcgatcgcg cgccgcacgc gcgacggtgg ccggtcgcgg
     1621 agcttcatcc gccgaagttg acttcgggga agaacgcctg gaagagctgc gtgtaggcca
     1681 ccgtcacgat cccgatggcc acgaagcagg tgaccaccga ctcgttgacg gcgcgcccca
     1741 caccctccgc cccgccctcg acctggaggc ccttctgcgc ggcgatgatg cccaccagcg
     1801 ccccgaacag cgcgagcttg accacgctgc cccacaggtc gatgaagttg gtcaggccca
     1861 gcagctggcc ggcgaaggcc tgcggcgtcg cgtcgtagag cagcacggtc gcggtgaacg
     1921 tgcccgcgat cccggcccag aaggtcagga gcgccagcac cggcatccac agggtcaggg
     1981 ccaccacgcg cggcaggacg aggttgcgga ccgggttgat gcccagcacc tcgagcgcgg
     2041 tcagctcgtc gcggatcgtg cgggcggcga tctccgccgt gatcgtcgcg cccagcgtgc
     2101 ccgcgacgat cgaggtcgtc agcagcggcc cgagctcccg gaggaagacc agcggggcca
     2161 ccggcgcgac ccggtccacc gcgccgaagg ccgaggccag cgacccgccc atgatcccga
     2221 tcgtgccgaa gccgacgacg aaggcgacga tcgacgcggg cgcgagcgtc agccggaagg
     2281 cgaacacgaa ctggcggagc agctcgttgc gccatgacag ccccgggctg acgagctggg
     2341 cgaacgtgcg catgcccagc ccgacggtct tgccggcgtt cgccacccac aggccggggg
     2401 gcacgtccgc gcgcgcgaac ggagcccacg gcgtgagcac tctcctctcc tccgaaccca
     2461 cccgaggaac ctacccttgg cgcatagcca ccatggctag cccccacact cccttaacga
     2521 tgtccgatgt ggccgcgcga cacccgcggg cgccccggca ggcgcgagga tcggacgcgt
     2581 gaccgcggcg gccgtcctac gcgagcaggg cggcgccttc gcgctggagg aggtggaggt
     2641 cgacgcgccg cgcgccggtg aggtgctggt gcgggtcgcc ggcgtgggga tctgccacac
     2701 ggacctggcc gccgccgacg gcgcgctggg tctcccgctg ccgatcgtgc tgggccacga
     2761 gggcgccggc acggtcgagg ccgtcgggcc gggcgtgacc gcggtggcac cgggcgatgc
     2821 cgtggtcctg acctacgacc actgcggcgc ctgcacgcgg tgcaccgcgg ggcacccggc
     2881 ctactgcgag cggttcgcgg ccctgaacgc atccggcgcc cgcgccgacg ggaccacgac
     2941 cctctccggc acgggcggcc cggtgcacgg cagcttctgc gggcagtcct cgttcgcggc
     3001 gctcgcgctg gcgggcgagc gcaacgcgat ccccgtgtcc accgatgccc cgctggagct
     3061 gctggggccg ctggggtgca gcgtccagac aggggcgggg gccgtgctcg aggtgctggg
     3121 cgcgcagccc ggtcagggga tcgccgtggt gggcctcggc gccgtgggcc tgtcggcggt
     3181 gatggccgcc gtggtcgccg ggtgcgaccc gatcgtggcc atcgaccccg accgctgccg
     3241 gcgcgagctg gccgaggagc tcggtgccac ccatgccctg gacggcgagc ggcgcccacg
     3301 cgccggcgtg gacctgtcgg tggacgcggt gggcacccag gatgcgctga ccgcggcact
     3361 gcgcgccctg gccgcgcccg gccactgcgc cacggtgggc tggcgcgggc cccgcaaccc
     3421 cctgacggtc gaccagggcc atctgttgca ggggcggtcg ctcaccggcg tgatcgaggg
     3481 cgacaccgtc cccggccggt tcatcccgca cctgctggag ctccacgcgg ccggccggtt
     3541 cccgttcgag cggctggtgc agaccgtccc cttcgaccgg atcggggagg ccgtggaggc
     3601 ggcgcgcgcg ggccgcgtgg tcaagcccgt gctggtgctc taggcgcgcc ggcgcaggaa
     3661 gaacaccagc ggagcgtcct cgccctt
//
LOCUS       DASUPW010000112         3232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1687665, whole genome shotgun sequence.
ACCESSION   DASUPW010000112 DASUPW010000000
VERSION     DASUPW010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1687665"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="VFY44_02540"
     CDS             complement(<1..1099)
                     /locus_tag="VFY44_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX5909342.1"
                     /translation="MHLGFALLTLFPGRVGGSEANVRGLVREFSAGNGPGAVTVLANA
                     EVARAYAGRVGGPVGLHHVRSYRAGDSAPTRLAAMAAAALAPRRAARDVPAGLDLLHH
                     PVTVPIPRLPGVPTVTTVYDVQHHDLPGLFSRAERGFRRWAYDGAARSADRVITTSAY
                     SASRLADAAGVPAERIEVIHMGVDHDRFTPSPTEADQRLRPRLPERYVLYPGNLWPHK
                     NHDRLVDALGAARDTDLALVLTGQDYGRLDALMARAERAGVRDRVHHLGYLPADEVPA
                     LMRGAQAMVFPSLYEGFGSPPLEAMACGCPVASSRRASLDEMVGDAVLDLDPESVAGI
                     AAAIDRITGDSELRSALRTRGLERAAGFRWAD"
     gene            1131..2147
                     /gene="gmd"
                     /locus_tag="VFY44_02545"
     CDS             1131..2147
                     /gene="gmd"
                     /locus_tag="VFY44_02545"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX5909343.1"
                     /translation="MPKALITGITGQDGSFLAEQLLDKGYEVHGLTRRSSSFNTERID
                     HLYRDIHEEEVRLFLHHADLSESSRLVRLVYEIQPDEIYHLGAQSHVRVSFDVPEYTS
                     DITGMGTVRLLEAIRDAQVPARFYQASSSEMFGATPPPQGENTPFHPRSPYAVAKVAA
                     YWITVNYRESYDMHASNGILFNHESERRGETFVTRKITRALAAIKLGLQNKLYLGNLD
                     AKRDWGYAPDFTDAMWRMLQQDTPDDYVIATGEMHSVRDFLTEAGRHLDLDWESVVEI
                     DPKYYRPAEVDALEGDATKAREVLGWEPKVKFAELVRIMCDADLKRLEDQLAGRGVRA
                     GDRG"
     gene            2140..3084
                     /locus_tag="VFY44_02550"
     CDS             2140..3084
                     /locus_tag="VFY44_02550"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEX5909344.1"
                     /translation="MAEPDPAFWQGKPVAVTGGAGFLGSAVTRDLQALGADVNVVRSA
                     DHDLRLPDQTRAAVDGAEVVVHLAANVGGIGFNRRNPAPLVHDNMAMTSNIFEQSRLA
                     GVSKLVSACTVCAYPKFVDAPFNEDDLWDGYPEESNAPYGLAKKMMLVLSDAYRRQYD
                     FDSCAPIVANLYGPNDNFDLEDSHVIAAMIRKYVEAKDGGQDTVTLWGTGEPSREFLY
                     VDDASRALLLAAERLDRSEPVNVGVGIETRIRDLAETIERLVGFEGETVWDTSRPDGQ
                     PKRFLDTSRAREWFGFEASVPLEEGLGRTIASFREQNG"
BASE COUNT          488 a         1161 c         1160 g          423 t
ORIGIN      
        1 cgtcggccca tctgaacccc gcagcgcgct cgaggcccct cgtgcgcagc gcagagcgta
       61 gctcggaatc gccggttatc cggtcgatgg ccgccgcgat gccggccacc gactcgggat
      121 ccaggtccag cacggcgtcg ccgaccatct cgtccagcga cgcccggcgc gacgaggcga
      181 cggggcagcc gcacgccatg gcctcgagcg gaggcgagcc gaacccctcg tacagcgagg
      241 ggaacaccat ggcctgggcg ccgcgcatca gcgcgggcac ctcgtcggcc ggcaggtagc
      301 ccaggtggtg cacgcggtcg cggacgccgg ctcgctccgc gcgcgccatc agcgcgtcca
      361 ggcggccgta gtcctggccc gtcaggacca gcgccaggtc ggtgtcgcgc gcggcgccga
      421 gggcatcgac cagccggtcg tggttcttgt gcggccacag gttgccgggg tagagcacgt
      481 agcgctcggg cagccggggg cgcagccgct ggtcggcctc ggtgggcgag ggggtgaagc
      541 ggtcgtggtc gacccccatg tggatgacct cgatccgctc ggccggcacc ccggccgcgt
      601 cggccagccg gctcgcggag taggcgctgg tggtgatcac ccggtcggcg gaccgggccg
      661 cgccgtcgta ggcccagcgc cggaacccgc gctcggcgcg cgagaacagc cctggcaggt
      721 cgtggtgctg cacgtcgtag accgtggtca ccgtgggcac gccgggcagg cgggggatgg
      781 gcacggtcac cggatggtgc agcaggtcca ggccggcggg cacgtcgcgc gccgccctcc
      841 gtggcgccag cgcggccgcg gccatcgccg cgagccgggt gggggccgaa tcgccggcgc
      901 ggtagctgcg cacgtggtgc aggccgaccg gtccgccgac ccggccggcg tacgcccggg
      961 ccacctcggc gttggccagc acggtcaccg cgccggggcc gttcccggcc gagaactcgc
     1021 gcaccaggcc gcgcacgttg gcctcggagc cgcccacgcg cccggggaac aacgtgagca
     1081 gggcgaagcc gaggtgcacc gctgcattgt gccgtctagg ctcccctgcc gtgcccaagg
     1141 cgctcataac aggcatcacc ggccaggacg gctcgttcct ggcggaacag ctgctcgaca
     1201 agggctacga ggtccacggc ctgacccggc ggtcctcgtc gttcaacacc gagcggatcg
     1261 accacctcta ccgcgacatc cacgaggagg aggtgcggct gttcctgcac cacgccgacc
     1321 tgagcgagtc ctcgcggctg gtgcgcctgg tgtacgagat ccagcccgac gagatctacc
     1381 acctgggtgc ccagtcccac gtgcgggtct cgttcgacgt gcccgagtac acgtccgaca
     1441 tcaccggcat gggcaccgtc cggctgctgg aggccatccg cgacgcccag gtgccggcgc
     1501 gcttctacca ggcgtcgtcg tccgagatgt tcggcgccac gccgccgccc cagggcgaga
     1561 acaccccctt ccacccgcgc agcccgtacg ccgtggcgaa ggtggcggcg tactggatca
     1621 ccgtgaacta ccgcgagtcg tacgacatgc acgcctccaa cgggatcctg ttcaaccacg
     1681 agtccgagcg ccgcggcgag accttcgtca cgcgcaagat cacccgcgcg ctggcggcca
     1741 tcaagctggg cctgcagaac aagctgtacc tgggcaacct ggacgccaag cgcgactggg
     1801 gctacgcgcc ggacttcacc gatgccatgt ggcgcatgct ccagcaggac acccccgacg
     1861 actacgtgat cgccacgggc gagatgcact cggtgcgcga cttcctcacc gaggccggca
     1921 ggcacctgga cctggactgg gagtccgtgg tcgagatcga cccgaagtac taccgcccgg
     1981 ccgaggtcga cgccctggag ggcgacgcca ccaaggccag ggaggtcctg ggctgggagc
     2041 ccaaggtcaa gttcgccgag ctggtgcgga tcatgtgcga cgccgacctc aagcgcctcg
     2101 aggaccagct ggccggccgc ggcgtccgcg cgggcgatcg tggctgagcc cgaccccgcc
     2161 ttctggcagg gcaagccggt cgcggtgacc ggcggcgccg gcttcctggg cagcgccgtc
     2221 acgcgcgacc tgcaggccct gggcgccgac gtcaacgtcg tgcgctcggc cgatcacgac
     2281 ctgcgcctgc ccgaccagac gcgcgcggcc gtggacggcg ccgaggtggt ggtgcacctg
     2341 gccgcgaacg tgggcggcat cggcttcaac cgccgcaacc cggcgccgct ggtgcacgac
     2401 aacatggcca tgaccagcaa catcttcgag cagtcgcgcc tggccggcgt gagcaagctg
     2461 gtctcggcct gcacggtctg cgcctacccc aagttcgtgg acgcgccgtt caacgaggac
     2521 gacctgtggg acggctaccc cgaggagtcc aacgcgccgt acgggctggc caagaagatg
     2581 atgctggtgc tgtccgacgc gtaccggcgc cagtacgact tcgactcctg cgcacccatc
     2641 gtggccaacc tctacgggcc gaacgacaac ttcgacctgg aggactcgca cgtgatcgcg
     2701 gccatgatcc gcaagtacgt ggaggccaag gacggcggcc aggacacggt cacgctgtgg
     2761 ggcacgggcg agccctcgcg cgagttcctg tacgtggacg acgcctcccg cgcgctgctg
     2821 ctggcggccg agcggttgga ccgttcggag cccgtgaacg tgggcgtggg catcgagacg
     2881 cgcatccgcg acctggccga gaccatcgag cggctggtgg gcttcgaggg cgagaccgtg
     2941 tgggacacct cgcgccccga cggccagccc aaacgcttcc tggacaccag ccgggcgcgc
     3001 gaatggttcg gcttcgaggc cagcgtgccg ctcgaggagg gcctggggcg caccatcgcc
     3061 tccttccgcg agcagaacgg ctgacgtggc ccggcgcgcg ctgatcaccg gcatcgccgg
     3121 gcaggacggc tcgtacctgg ccgagctgct gctggagaag ggctacgagg tgttcggcat
     3181 cgtccggggg tcgaccgagg gctggtaccc caacctcgag ggcgtccgcg ac
//
LOCUS       DASUPW010000113         7937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_442395, whole genome shotgun sequence.
ACCESSION   DASUPW010000113 DASUPW010000000
VERSION     DASUPW010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7937
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442395"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VFY44_02555"
     CDS             <1..688
                     /locus_tag="VFY44_02555"
                     /inference="COORDINATES: protein motif:HMM:NF006100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX5909345.1"
                     /translation="VNAAIAEGVAEALDRLDSDDGLSVGVLTGAGRGFCAGMDLKAFV
                     TGERPWVGDRGFAGITQRGSRKPLIAAIEGFAVAGGLEVALACDLVVAATGAKLGIPE
                     AKRGLVAAGGALLHLPGRIPYHVAMELALTGDPITAERGYELGLVNRIAEPDHAVTEA
                     LTLAAEIAANGPLALIGSKRIVQESRDWPLDQMWERQAEISEPILASEDAQEGSKAFA
                     EKRDPEWRGV"
     gene            699..1226
                     /locus_tag="VFY44_02560"
     CDS             699..1226
                     /locus_tag="VFY44_02560"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX5909346.1"
                     /translation="MDESLRGQLLIAAPQLSDYFRRTVVLVLEHTEDGAMGLVLNRPT
                     ESEVIEAVPSLSSLVDGSDLVHAGGPVQPDSVLVLGDFEEPGDAGTQVVGTLGLLDPE
                     HPEAELNRVRVFAGYAGWAPGQLDLELEEEAWIVAPADPDDAFSEDDLWGEALRRKGG
                     EYALLATMPADPSLN"
     gene            1244..1432
                     /locus_tag="VFY44_02565"
     CDS             1244..1432
                     /locus_tag="VFY44_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909347.1"
                     /translation="MATRVCPNCGESVDQSDASTTDSDGDQHGEGQGEQERVQCPNCD
                     ATLTRAAGAGGPWVLAKD"
     gene            complement(1512..2162)
                     /gene="msrA"
                     /locus_tag="VFY44_02570"
     CDS             complement(1512..2162)
                     /gene="msrA"
                     /locus_tag="VFY44_02570"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX5909348.1"
                     /translation="MFEFLTRKTEIPAPEDALPGRDTAIVQPGDHAVLGTPMAPPFPD
                     DTEQVIVGMGCFWGAEKAFWQLPGVYTTAVGYSGGITPNPTYEEVCSGRTGQNEVVLV
                     VYKPSEVSFEDVLKAFWEGHDPTQGMRQGNDVGTQYRSAIYTTTDAQAEAVEASRAAY
                     QAELEKAGHGQITTEVAPAGEFYYAEDYHQQYLHKVPNGYCGLGGTGVSCPVGLSA"
     gene            complement(2212..3402)
                     /locus_tag="VFY44_02575"
     CDS             complement(2212..3402)
                     /locus_tag="VFY44_02575"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX5909349.1"
                     /translation="MHQPAVTEIAEELEFERNILRELLKQAPTAVIVTWGRELRFRYI
                     SDRSLEFMPYERDELIGRTVDEVFPNAAEAIEEVRVAILDRGETVEVHDVPLAASGPD
                     AWEGDRYYTFISSPVEGPDGAPAGILNVGYETTADVRARKLLERELATEQRIATQLQV
                     SLMPDSLPDVPGMDLASGFRPAGNGHEIGGDFYDVFRVRDDCWMVVVGDVCGKGAEAA
                     AITALARYTMRAAAIRDGADPCTVHARLNEALLRQREDLRFVSAVCAFLEPLEGGGAT
                     LSVCVAGHLPPLRVGSGGTVTRIAGGHGVVLGIWDQPDLEQERVELAPGERLVLYTDG
                     VLDADPEHELTEAAFGDMLGRTRDDEAAADTVSLIEREVVQGGALRGRDDVAVLVIRA
                     HDQG"
     gene            3469..4539
                     /locus_tag="VFY44_02580"
     CDS             3469..4539
                     /locus_tag="VFY44_02580"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909350.1"
                     /translation="MPRLKRVDPGGPGITRRRRGRGWQYLDEDGRKVQDPEVVARIQA
                     LAIPPAWQDVWITVEPMGHIQATGTDAAGRKQYRYHDKWRERRDAEKFESMILFAKSL
                     PRLRKRVARDLKAGDELGRERVLACATRLLDKGFFRVGSEGYAEENETYGLATILKSH
                     VTIDGSAMRFDYSAKGNKRRVQRVNDPDIAGLVETLKRRRSGGDELLAYKRGSRWVDV
                     KSTDVNEYLKAAAGGDFSAKDFRTWNATVLAALALAPSGEAAMASKTARKRAIKRAID
                     ETARYLGNTPAVCRASYIDPRVFDRFQGGLTIAGVLPELVDPDTDWPEIQPTVEAGVL
                     ALIADDEEAPAVEKGPVVEDAA"
     gene            complement(4536..5282)
                     /locus_tag="VFY44_02585"
     CDS             complement(4536..5282)
                     /locus_tag="VFY44_02585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HEX5909351.1"
                     /translation="MSSPELPSASEQELLRRYAAGDVSAREELARSYMPLARRLAGRY
                     RHTGEAQDDLEQVAYLGLLKAIDRYDAGRGPFVRYAVPNIMGELKRHFRDKGWGLHVP
                     RSVQERVLVVNRASDTLAGKLGRSPSPRDLAEATGLTMEQVLEALDAASAYTPAALDA
                     PQPGGDGDGERTLGDTLGGDDAHYELVELGEAIAPAFQALPEREQVILKLRFIDDLTQ
                     SEIAEKIGVSQMHVSRLLRRSLNQLSEAGR"
     gene            complement(5486..5668)
                     /locus_tag="VFY44_02590"
     CDS             complement(5486..5668)
                     /locus_tag="VFY44_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909352.1"
                     /translation="MDEREESDQLPEEGPGGQVPEDDSDGKARSDAQENSGAEGEEGG
                     SAGKQATGNPANAGQE"
     gene            complement(5671..5961)
                     /locus_tag="VFY44_02595"
     CDS             complement(5671..5961)
                     /locus_tag="VFY44_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909353.1"
                     /translation="MPADEREEEQAAAEEAGAIGGPNPDPELSDAERVISEGGEGESE
                     GFELAERDLERNATHDPDGHGNPLLDRIDEESEDSGAEYGEADHEGNQEEGA"
     gene            complement(5961..6464)
                     /locus_tag="VFY44_02600"
     CDS             complement(5961..6464)
                     /locus_tag="VFY44_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5909354.1"
                     /translation="MATTASQGILGEDAREDREKIVEMLKKAYWMELETVMSYIANSV
                     NPDGLLAREISESLDQDIQEELGHAQQFAARIKELYGVVPGSKEFAAEQDFLQPPGEQ
                     TDLAHVVDGVIAAETGAIEHYNALIEFCADRDPVTEDMVIAILRDEEGHRRLFEGFKR
                     GLEATYS"
     gene            complement(6497..7333)
                     /locus_tag="VFY44_02605"
     CDS             complement(6497..7333)
                     /locus_tag="VFY44_02605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5909355.1"
                     /translation="MDEAAETGEALDLFMRDIRRYPLLTKQEEVDLARRVEQGDLAAK
                     ERMINSNLRLVVSVAKRYQGHELPLSDLIQEGMLGLIRASEKFDYRKGFKFSTYATFW
                     IRQAIQRGLANKARTIRVPVHIGQHERRIARVEDKLATELGREPTYEELAAAAELEPR
                     QVREVLEAPRASTSLERPVGEEGSSSLGDLLPSDAAGPAEQVGDAMESDAVRGAVMDL
                     PERERAVIGMRFGLDDGDPKPLREAGRRLGLSSEGVRKIEAQALRRLSECASLGAAPG
                     TA"
     gene            7352..>7937
                     /locus_tag="VFY44_02610"
     CDS             7352..>7937
                     /locus_tag="VFY44_02610"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX5909356.1"
                     /translation="MNGTKHSEPEHLRHLGARTWWATLKRTVREFQDDELMDRAAALT
                     YYGVLALFPALIALVSIVGLFGDPQAVTDTITEVIEELAPGSASDTLQDTIEGLTQDR
                     GAAGVLLVLGILGALNAASGYIGAFIRASNVIYEVQEGRPFYKLRPLQLAVTIVIVSA
                     VGLVIMALVLSGPLAGAVGDAIGLSEEAVTAYGVA"
BASE COUNT         1041 a         2898 c         2826 g         1172 t
ORIGIN      
        1 ggtcaacgcg gcgatcgccg agggcgttgc cgaggcgctg gaccggctgg actccgacga
       61 cgggctgtcc gtcggggtgc tcacgggggc gggccggggc ttctgcgccg gcatggacct
      121 gaaggcgttc gtcaccggcg agcggccgtg ggtcggcgac cgcggcttcg ccggcatcac
      181 ccagcgcggc tcgcgcaagc cgctgatcgc ggccatcgag ggcttcgccg tggccggcgg
      241 gctcgaggtg gcgctggcct gcgacctggt ggtggccgcc accggcgcca agctcggcat
      301 ccccgaggcc aagcgcggcc tggtggcggc cggcggcgcc ctgctgcacc tgcccggccg
      361 gatcccctac cacgtggcca tggagctggc gctgaccggt gacccgatca cggccgagcg
      421 cggctacgag ctgggcctgg tcaaccggat cgccgagccc gaccacgccg tgaccgaggc
      481 gctgacgctg gccgccgaga tcgcggccaa cgggccgctg gcgctgatcg gctcgaagcg
      541 gatcgtccag gagtcgcgcg actggccgct cgaccagatg tgggagcgcc aggccgagat
      601 ctccgagccg atcctggcct ccgaggacgc ccaggagggc tcgaaggcct tcgccgagaa
      661 gcgcgacccg gagtggcggg gcgtctaggg ttggacccat ggacgagtcg ctccgtggcc
      721 agctgctgat cgccgccccc cagctgagcg actacttccg ccgcacggtc gttctcgtcc
      781 tcgagcacac cgaggacggg gccatgggcc tggtgctcaa ccgccccacc gagtccgagg
      841 tcatcgaggc cgtgccgtcg ctctcgagcc tggtcgacgg cagcgacctg gtccacgcgg
      901 gcggtcccgt gcagcccgac tccgtgctgg tgctgggcga cttcgaggag cccggcgacg
      961 ccggcaccca ggtggtcggc acgctgggcc tgctcgaccc cgagcacccc gaggccgagc
     1021 tcaaccgcgt ccgcgtgttc gccggctacg ccggctgggc tcccggccag ctggacctgg
     1081 agctggagga ggaggcctgg atcgtcgccc ccgccgaccc cgacgacgcg ttcagcgagg
     1141 acgacctctg gggcgaggcg ctgcgacgca agggcggcga gtacgccctg ctggcgacca
     1201 tgccggccga tccgtccctg aactaggggc ctacactcgg tcgatggcca cgcgcgtatg
     1261 tcccaactgc ggcgagtccg tcgaccagag cgacgcctcg accaccgact ccgacggcga
     1321 ccagcacgga gaggggcagg gcgagcagga gcgggtccag tgcccgaact gtgacgccac
     1381 gctgaccagg gccgcagggg ccggcggccc ctgggtcctg gccaaggatt gatcttgcgg
     1441 ccatccctgg ccgcgctctg atgacctagg ggccgtcccg gccccgtgaa cgggcgggct
     1501 tcgccgccct cctacgcgct caaccccacc gggcagctca cgccggtccc gccgaggccg
     1561 cagtagccgt tggggacctt gtgcaggtac tgctggtggt agtcctcggc gtagtagaac
     1621 tcgcccgccg gcgccacctc ggtcgtgatc tggccgtggc ccgccttctc cagctcggcc
     1681 tggtaggccg cgcgggaggc ctccaccgcc tcggcctggg cgtcggtggt ggtgtagatg
     1741 gccgaccggt actgggtgcc cacgtcgttg ccctggcgca tgccctgggt cgggtcgtgt
     1801 ccctcccaga acgccttcag cacgtcctcg aaggagacct ccgagggctt gtagaccacc
     1861 agcaccacct cgttctgccc ggtgcgcccc gaacagacct cctcgtaggt ggggttgggg
     1921 gtgatcccgc cgctgtagcc gacggcggtg gtgtacacgc cgggcagctg ccagaacgcc
     1981 ttctccgcgc cccagaagca gcccatgccc acgatgacct gctcggtgtc gtcggggaac
     2041 ggcggcgcca tcggggtgcc cagcacggcg tggtcgcccg gctggacgat ggccgtgtca
     2101 cgcccgggaa gggcgtcctc gggggcgggg atctcggtct tgcgggtcag gaactcgaac
     2161 attcagtcct cctcgggatc ccctccaagg ttagtccccg gtgtccacgc ctcagccctg
     2221 gtcgtgggcc cggatcacca gcacggccac gtcgtcgcgg ccccgcagcg cgcccccctg
     2281 gaccacctcg cgctcgatca gcgagaccgt gtcggcggcc gcctcgtcgt ccctcgtccg
     2341 gccgagcatg tcgccgaacg ccgcctcggt gagctcgtgc tcggggtcgg cgtccagcac
     2401 gccgtcggtg tagagcacca gccgctcgcc gggggcgagc tcgacccgct cctgctccag
     2461 gtcgggctgg tcccagatgc ccagcaccac cccgtgaccg ccggcgatgc gcgtcacggt
     2521 gcccccgctg cccacgcgca gcggcggcag gtggccggcg acgcacacgc tcagcgtggc
     2581 cccgccgccc tccagcggct ccaggaaggc gcacaccgcg gacacgaagc gcaggtcctc
     2641 gcgctggcgc agcagcgcct cgttgagccg cgcgtgcacg gtgcacgggt cggcgccgtc
     2701 gcggatggcc gccgcgcgca tggtgtaccg cgccagcgcc gtgatggccg cagcctccgc
     2761 gcccttgccg cagacgtcgc ccaccaccac catccagcag tcgtcgcgca cgcggaagac
     2821 gtcgtagaag tcgccgccga tctcgtgccc gttgcccgcc gggcggaagc ccgacgccag
     2881 gtccatgccc gggacgtcgg gcaggctgtc gggcatcagg ctgacctgga gctgagtggc
     2941 gatccgctgc tcggtggcca gctcgcgctc cagcagcttg cgcgcgcgca cgtccgccgt
     3001 ggtctcgtag cccacgttca ggatgcccgc gggggcgccg tccgggccct cgacgggtga
     3061 ggagatgaag gtgtagtagc ggtcgccctc ccacgcgtcg ggcccgctgg cggccagcgg
     3121 cacgtcgtgc acctcgacgg tctcgccgcg gtcgaggatg gccacccgga cctcctcgat
     3181 ggcctccgcg gcgttgggga agacctcgtc gaccgtgcgg ccgatgagct cgtcgcgctc
     3241 gtagggcatg aactccagcg agcgatcgct gatgtagcgg aaccggagct cgcggcccca
     3301 ggtcacgatg acggccgtgg gcgcctgctt gagcagctcc cgcaggatgt tccgctcgaa
     3361 ctcgagctcc tcggcgatct cggtgacggc gggctggtgc acgtagcgtg aaaactaccg
     3421 gcggggcctc gtttgcgggg gatctcccgt gggtagccga gcgcgaccat gccacggctc
     3481 aagagagtgg accccggcgg ccccgggatc acccgccgcc gccgcggccg cggatggcag
     3541 tacctggacg aggacggccg caaggtgcag gacccggagg tggtggcccg gatccaggcg
     3601 ctggccatcc cgcccgcgtg gcaggacgtg tggatcaccg tcgagccgat gggccacatc
     3661 caggccaccg gcacggacgc cgcgggccgc aagcagtacc gctaccacga caagtggcgc
     3721 gagcgccggg acgcggagaa gttcgagtcg atgatcctgt tcgccaagtc cctgccgagg
     3781 ctgcgcaagc gcgtcgcacg cgatctgaag gccggcgacg agctgggccg cgagcgcgtg
     3841 ctggcctgcg cgacccggct cctggacaag ggcttcttcc gcgtcggctc ggagggctac
     3901 gcagaggaga acgagaccta cgggctggcc acgatcctca agagccacgt gaccatcgac
     3961 ggcagcgcga tgcggttcga ctactccgcg aagggcaaca agcggcgggt gcagcgggtg
     4021 aacgacccgg acatcgccgg gctggtcgag acgctcaagc ggcgccgctc gggcggcgac
     4081 gagctgctgg cctacaagcg cggcagccgc tgggtggacg tgaagtccac agacgtgaac
     4141 gagtacctca aggccgccgc cggcggcgac ttctccgcca aggacttccg cacgtggaac
     4201 gccaccgtgc tggccgcgct ggccctggcg ccgtcgggcg aggccgccat ggcgtccaag
     4261 acggcgcgca agcgggccat caagcgcgcg atcgacgaga ccgcccgcta cctgggcaac
     4321 acgccggcgg tgtgccgggc gtcgtacatc gacccgcgcg tcttcgaccg cttccagggc
     4381 ggcctgacca tcgccggggt cctgccggag ctggtggacc cggacaccga ctggcccgag
     4441 atccagccca ccgtcgaagc cggcgtgctg gcgctgatcg ccgacgacga ggaggccccc
     4501 gccgtggaga agggcccggt ggtggaggac gcggcctagc gcccggcttc gctgagctgg
     4561 ttcagcgacc ggcgcagcaa ccgcgagacg tgcatctggg agacgccgat cttctcggcg
     4621 atctccgact gggtcaggtc gtcgatgaag cgcagcttca ggatcacctg ctcgcgctca
     4681 ggcagggcct ggaacgcggg cgcgatcgcc tcccccagct cgaccagctc gtagtgggcg
     4741 tcgtcgccgc cgagggtgtc gcccagcgtg cgctcgccgt cgccgtcgcc gcccggctgg
     4801 ggcgcgtcca gcgcggccgg cgtgtacgcg ctggcggcgt ccagggcctc gagcacctgc
     4861 tccatggtca gccccgtcgc ctcggcaagg tcgcgcgggc tgggcgagcg gcccagcttc
     4921 ccggccagcg tgtcggaggc ccggttgacc acgagcaccc gctcctgcac cgagcgcgga
     4981 acgtgcaggc cccagccctt gtcgcggaag tggcgcttga gctcgcccat gatgttgggc
     5041 accgcgtagc gcacgaacgg cccgcggccg gcgtcgtagc ggtcgatggc cttgagcagg
     5101 cccaggtagg ccacctgctc cagatcgtcc tgggcctcgc cggtgtggcg gtagcgcccg
     5161 gccagccgcc gggccagcgg catgtagctg cgggccagct cctcgcgcgc ggagacgtcc
     5221 ccggccgcgt agcggcgcag gagctcctgc tcgctcgcgg acggcagctc gggggacgac
     5281 atcacgggaa agctacggac cctgaggccc ggcggattcc cgggtcggcg ctagccgaca
     5341 ggttcgggct ccggaacctc cggtggctgc accggcgggg cgggctccgg ggcgttgggc
     5401 gtgtccggct cgggggccgg cgggtcgacc gggacgtcgg ggacctcggg gccgaccggc
     5461 gatgggggct cgggcatcct catggctact cctgccccgc gttggcgggg ttgccggtgg
     5521 cctgcttgcc ggcgctgccg ccctcttcgc cctcggcgcc cgagttctcc tgggcgtcgc
     5581 tccgggcctt gccgtccgag tcgtcctcgg ggacctgtcc tccggggccc tcctccggca
     5641 gctggtcgga ctcttcgcgc tcatccatgg ctatgcgccc tcttcctggt tgccctcgtg
     5701 gtcggcctcg ccgtactcgg cgccggagtc ctcggactcc tcgtcgatgc ggtccagcaa
     5761 cgggttgccg tggccgtcgg ggtcgtgcgt cgcgttgcgc tccagatccc gctcggccag
     5821 ctcgaagccc tcggactcgc cctcgccgcc ctcggagatc acgcgctcgg cgtcgctgag
     5881 ctccgggtcg gggttcgggc cgccgatggc acccgcctcc tcggcggccg cctgctcctc
     5941 ttcgcgttcg tcggccggca tcacgagtac gtggcctcga ggccgcgctt gaagccctcg
     6001 aacaggcgcc ggtggccttc ctcgtcgcgc aggatcgcga tgaccatgtc ctcggtcacg
     6061 ggatcacggt cggcgcagaa ctcgatcagg gcgttgtagt gctcgatggc gccggtctcg
     6121 gcggcgatca cgccgtcgac cacgtgggcc aggtcggtct gctcgccggg gggctgcagg
     6181 aagtcctgct cggccgcgaa ctccttcgag cccggcacca cgccgtagag ctccttgatc
     6241 cgggcggcga actgctgcgc gtgacccagc tcctcctgga tgtcctggtc caggctctcg
     6301 gagatctcgc gggccagcag cccgtcgggg ttcacggagt tggcgatgta gctcatgacc
     6361 gtctcgagct ccatccagta cgccttcttg agcatctcca cgatcttctc gcggtcttcg
     6421 cgggcgtcct caccgaggat gccctgactg gcggtggttg ccatgccagt tcctttctac
     6481 gggtgcaagt ccaacactag gccgtacccg gagcggcacc cagtgatgca cactcggaca
     6541 gccggcgcag ggcctgcgcc tcgatcttgc gcacgccctc cgacgacagg cccagccgcc
     6601 gtcccgcttc acgcaggggc ttggggtcgc cgtcgtccag cccgaagcgc atgccgatca
     6661 cggcgcgctc gcgctcgggg aggtccatga ccgcgccccg gaccgcgtcg ctctccatgg
     6721 cgtcgcccac ctgctcggcc ggcccggcgg cgtcgctggg aagcaggtcg ccgaggctcg
     6781 acgagccctc ctcgcccacc gggcgctcca ggctggtgga ggcccgcggg gcctccagca
     6841 cctcgcgcac ctggcggggc tccagctcgg ccgcggcggc cagctcctcg taggtcggct
     6901 cgcgccccag ctcggtggcc agcttgtcct cgacccgggc gatgcggcgc tcgtgctggc
     6961 cgatgtgcac gggcacccgg atggtgcggg ccttgttggc cagcccgcgc tggatggcct
     7021 ggcggatcca gaaggtcgcg taggtcgaga acttgaagcc cttgcggtag tcgaacttct
     7081 ccgaggcgcg gatcaggccc agcatgccct cctggatcag gtcgctcagc gggagctcgt
     7141 ggccctggta gcgcttggcc accgacacca ccaggcgcag gttcgagttg atcatccgtt
     7201 ccttggccgc caggtccccc tgctcgacac gccgggccag gtcgacctcc tcctgtttgg
     7261 tcaggagggg gtaccgccgg atgtcgcgca tgaacaggtc cagcgcctcg cccgtctcgg
     7321 ccgcctcgtc catgatgctt tccttcccac ggtgaacggc accaaacact ccgagccgga
     7381 gcatctacgc cacctcggag cgcgaacgtg gtgggccacg ctcaagcgca ccgtccggga
     7441 gttccaggac gacgagctga tggaccgggc cgcggccctg acctactacg gcgtcctggc
     7501 cctgttcccc gccctcatcg ccctcgtgtc gatcgtcggc ctgttcggcg acccccaggc
     7561 cgtgaccgac acgatcaccg aggtcatcga ggagctggcc ccgggctcgg ccagcgacac
     7621 cctgcaggac acgatcgagg gcctgaccca ggaccgcggc gccgccggcg tcctgctggt
     7681 gctgggcatc ctgggcgccc tgaacgcggc ctcgggctac atcggcgcct tcatccgcgc
     7741 ctcgaacgtg atctacgagg tgcaggaggg gcgccccttc tacaagctgc gcccgctcca
     7801 gctggcggtc acgatcgtca tcgtcagcgc cgtcggcctg gtgatcatgg ccctggtgct
     7861 cagcggcccc ctggccggcg cggtcggcga cgcgatcggc ctcagcgagg aggcggtgac
     7921 cgcgtacggc gtggcca
//
LOCUS       DASUPW010000114         2554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_718882, whole genome shotgun sequence.
ACCESSION   DASUPW010000114 DASUPW010000000
VERSION     DASUPW010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718882"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /gene="menD"
                     /locus_tag="VFY44_02615"
     CDS             complement(<1..607)
                     /gene="menD"
                     /locus_tag="VFY44_02615"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HEX5909357.1"
                     /translation="MIPINSTYAPVQAMVDELARCGTTHAVTCPGSRNAPVVLAVAAD
                     ERLTAVSVIDERVAGFMALGMAKATGRPVVVTCTSGTAAANLAPAVHEAWQARVPLIV
                     LTADRPPELRDVGAGQAIDQIGLYGSAAKWFVELGNGEPGPGWNAHVRSIACRAVATA
                     AGGRPGPVHLNLPLREPLAPVPEELPAEHWQGRAGGAPWTEV"
     gene            614..1327
                     /locus_tag="VFY44_02620"
     CDS             614..1327
                     /locus_tag="VFY44_02620"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5909358.1"
                     /translation="MQIVFTPGFMQPASAWEAVAGQLGERYSSVMVDHHEHSFEGRVG
                     ELREAAGGAVLCGYSLGGRLALHAALRDPGAYAGLVLVGTSAGIDEPVARAERARTDE
                     KLASWMETQPIESIVDIWERQPLFADQSDSLVEAQKPGRLAQDPRSLALILRTAGQGV
                     LEPVWGRLSGLDVPVLAMAGDRDQRYSDAARRIAREVPDGRAEIIEHAGHAPQLQQPE
                     AVARVLADFLDQHFGQRVV"
     gene            complement(1283..2299)
                     /locus_tag="VFY44_02625"
     CDS             complement(1283..2299)
                     /locus_tag="VFY44_02625"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HEX5909359.1"
                     /translation="MKLRTLLRLAIPLREPFATANGVLAERELLVIRLEDDDGTVGFG
                     EAAPLEPYDGVSIDQVIDALREGPPPRDAPRQARAGWELAELDLQRRRLGRPLGEPLH
                     DQIPVNLTLPAGPADEVADAAKAGLRKGYSCFKVKVGLPDDAERVRAVREAVGPWPAI
                     RVDANGAWTPDQAVAALAELAPLDLELVEQPCATLEELAEVRARVEVTVAADESIAGA
                     EDVHAAAALGACDAVNVKLAAAGGFGPARDALRAAREHHLAAWLSSTLDGPWGIAAGL
                     QLASSANLGMACGLATLELFDARLARALPSPSTGLMAVPQGPGLGLDVSDDALAEVLV
                     EEVR"
     gene            complement(2296..>2554)
                     /locus_tag="VFY44_02630"
     CDS             complement(2296..>2554)
                     /locus_tag="VFY44_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909360.1"
                     /translation="LGRERARRLFDAMLALSYAMCIVIPAAGGLTWWTLLPLLSIPLA
                     LPLRKTVATRTDGPALNGALAGAGRLLGVFSALLAVGVLLS"
BASE COUNT          323 a          948 c          980 g          303 t
ORIGIN      
        1 ggacctccgt ccagggcgcc ccgcctgctc gtccctgcca gtgctcggcg ggcagctcct
       61 cggggacggg ggccagcggc tcgcgcaggg gcaggttcag gtgcaccggc cccgggcgtc
      121 ccccggcggc ggtggcgacc gcgcggcagg cgatcgagcg cacgtgggcg ttccagcccg
      181 gccccggctc gccgttgccc agctcgacga accacttcgc ggcggacccg tacagcccga
      241 tctggtcgat ggcctgcccg gcgccgacgt cacgcagctc gggcgggcgg tcggcggtca
      301 ggacgatcag cggcacgcgg gcctgccagg cctcgtggac cgccggcgcc aggttggcgg
      361 ccgccgtgcc cgaggtgcat gtcaccacca ccgggcggcc ggtggccttg gccatgccta
      421 gggccatgaa gccggccacg cgctcgtcga tgaccgacac cgcggtcagg cgctcgtcgg
      481 ccgccacggc cagcacgacc ggggcgttgc gcgaccccgg acaggtcacg gcgtgcgtcg
      541 tgccgcagcg cgccagctcg tcgaccatgg cctggacggg cgcgtacgtg ctgttgatag
      601 ggatcattgg cccatgcaga tcgtcttcac acccggattc atgcagcccg ccagcgcgtg
      661 ggaggcggtc gccgggcagc tgggcgaacg ctactccagc gtgatggtcg accatcacga
      721 gcactcgttc gaaggacgtg tgggcgagct gcgggaggcc gcagggggcg cggtcctgtg
      781 cggctactcc ctgggtgggc ggctggcgct gcacgcggcc ctgcgtgatc ccggcgccta
      841 cgcgggcttg gtgctggtcg ggacctcggc gggcatcgac gagcccgtgg cccgggccga
      901 gcgtgcccgc acggacgaga agctggcctc gtggatggag acgcagccga tcgagtcgat
      961 cgtggacatc tgggagcgcc agccgctgtt cgccgaccag tcggacagcc tggtggaggc
     1021 ccagaagccc ggacgcctgg cccaggaccc gcgctcgctg gccctgatcc tgcgcactgc
     1081 cggccagggc gtgctggagc ccgtctgggg ccggctgagc ggcctggacg tcccggtgct
     1141 ggccatggcc ggcgaccgcg accagcgcta ctcggacgcc gcccggcgga tcgcgcgcga
     1201 ggtcccggac gggcgcgcgg agatcatcga gcatgcgggg cacgcgcccc agctccagca
     1261 gcccgaggcc gtcgcgcggg tgctagcgga cttcctcgac cagcacttcg gccagcgcgt
     1321 cgtctgagac gtccaggccc agccccgggc cctgcggcac ggccatgagc ccggtggagg
     1381 gggacggcag ggcgcgggcc aggcgcgcgt cgaagagctc gagcgtggcc aggccacagg
     1441 ccatgcccaa attggccgac gaggccagct gcagccccgc cgcgatgccc cagggcccgt
     1501 ccagggtgct ggacagccac gccgccagat ggtgctcgcg ggcggcccgc agcgcgtcgc
     1561 gcgcgggccc gaagcccccg gccgcggcca gcttcacgtt cacggcgtcg cacgcgccca
     1621 gcgccgccgc ggcgtgcacg tcctccgctc cggcgatcga ctcgtcggcg gccacggtga
     1681 cctcgacgcg ggcgcgcacc tcggccagct cctcgagcgt ggcgcagggc tgctccacca
     1741 gctccaggtc cagcggcgcc agctcggcca gtgccgccac ggcctggtcc ggtgtccagg
     1801 cgccgttggc gtccacccgg atcgcgggcc acgggccgac ggcctcgcgc acggcgcgca
     1861 cgcgctcggc gtcgtcggga agcccgacct tgaccttgaa gcaggagtag cccttgcgca
     1921 ggccggcctt cgcggcgtcc gcgacctcgt ccgcgggccc ggccggcagc gtcaggttca
     1981 ccgggatctg gtcgtgaagc ggctcgccga gggggcggcc gagccggcgc cgctggaggt
     2041 ccagctccgc cagctcccag ccggcgcggg cctggcgtgg cgcgtcgcgc gggggcggcc
     2101 cctcgcgcag ggcgtcgatc acctggtcga tgctcacgcc gtcgtagggc tccaggggcg
     2161 cggcctcgcc gaagccgacc gtcccgtcgt cgtcctcgag gcggatcacc aggagctcgc
     2221 gctcggccag gacgccgttg gccgtggcga agggctcgcg cagcgggatc gccagccgga
     2281 gcagggtccg gagcttcacg agagcagcac gcccaccgcc aggagggcgc tgaagacacc
     2341 gagcaggcgg ccggcgccgg ccagcgcgcc gttcagcgcc ggcccgtcgg tgcgggtggc
     2401 cacggtcttt cgcaggggca gggccagcgg gatcgacagc agcggcagca gcgtccacca
     2461 ggtcagcccg ccggcggcgg ggatgacgat gcacatggcg taggagagcg ccagcatggc
     2521 gtcgaacagg cgccgcgcgc gctcacggcc cagc
//
LOCUS       DASUPW010000115         2386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_580457, whole genome shotgun sequence.
ACCESSION   DASUPW010000115 DASUPW010000000
VERSION     DASUPW010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580457"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..1596
                     /locus_tag="VFY44_02635"
     CDS             274..1596
                     /locus_tag="VFY44_02635"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5909361.1"
                     /translation="MSQKSFADLGVSNAVVRALSERGYDEPFAVQRLVIEDILDGRDV
                     LAQSPTGSGKTLAFGVPMADLIDAESRRPAGLILAPTRELALQIVEELQAVAGARALS
                     VAVVHGGVGLVAQAKKAAKAHIVVATPGRLEDLMQRGAISLKHVQILVLDEADRMLDM
                     GFKPAVDRIVGKTPDDRQTLFFSATLQGEVGQQAKAFTHDPAKHTHTPPAESKGDISH
                     RFVHVSHEAKVDRLVRELSESERGRALVFVRTKRGADRLVKKLDREKVRAVAMHGNKS
                     QAQRQKALAAFEKGTVDTLVATDVAARGIDVPDITHVINFDAPDDRDTYTHRVGRTGR
                     AGNTGAGVSFVLGDQVHDMRKIARDLGLGDQFDAADGTPVAVPSDGPNTNRTRPQRGG
                     GGGGGRNGNRSRGGGGGQGGGNRPKAKSGAQGWSSDGRGPKRQGRRSR"
     gene            1648..>2386
                     /locus_tag="VFY44_02640"
     CDS             1648..>2386
                     /locus_tag="VFY44_02640"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5909362.1"
                     /translation="MSQAALLERDRELATLEPVVDEAASGEGRVVVFEGPAGIGKTRL
                     LGETRRLAEAAGMRVLSARGGALEREYPYGVVRQLFEPVLVDPEARERLLGESAALAR
                     PVFEGADAGAPDSGEFSTLHGLYWLTVNLATERPLMLALDDLQWCDRPSLRFVAYLNR
                     RIEGMGLLVAATRRDAEPGTDPALLAEITGDPSATSLRPQPLSPTAGRELVSAGLGRE
                     PDERFTAACQKATGGNPLLLGQLVSALR"
BASE COUNT          324 a          887 c          865 g          310 t
ORIGIN      
        1 gaggagttcg accacctgct gttcgcccac ggcgatccgg tggtcggcgg gggccgcgag
       61 gcgctggtcg ccctggtgca aaaccccagc tgagcgggcg attccgcgcg cggctgctac
      121 cgtccgcggg gtcgggttgc caaccgtggc gcccgagagg ttccgttcct agctgcgtga
      181 gtaggtgcgc cggattctgc ctcgtgcagt tatccggccg gtgcgcgtcc ggggtcgctg
      241 gatctccccg acgtacacat aaggagcagt tccatgtctc agaagtcttt cgccgatctc
      301 ggcgtgtcca acgcggtcgt acgcgcgctc tccgagcgcg gctacgacga gcccttcgcc
      361 gtccagcgcc tggtcatcga ggacatcctc gacggccgcg acgtgctggc ccagtccccg
      421 accggctccg gcaagacgct ggccttcggc gtgccgatgg ccgacctgat cgacgccgag
      481 tcgcgccgcc ccgccggcct gatcctggcg cccacccgtg agctggcgct ccagatcgtg
      541 gaggagctgc aggccgtggc cggcgcccgc gcgctgtccg tggccgtggt gcacggcggc
      601 gtcggtctcg tggcccaggc caagaaggcc gccaaggccc acatcgtggt ggccaccccc
      661 ggccgcctcg aggacctgat gcagcgcggc gccatcagcc tcaagcacgt gcagatcctg
      721 gtcctggacg aggccgaccg gatgctggac atgggcttca agcccgccgt cgaccggatc
      781 gtgggcaaga cgcccgacga ccgtcagacg ctgttcttct cggccacgct ccagggcgag
      841 gtcggccagc aggccaaggc cttcacccac gacccggcca agcacacgca cacgccgccg
      901 gccgagagca agggcgacat cagccaccgc ttcgtgcacg tctcgcacga ggccaaggtc
      961 gaccgcctgg tgcgcgagct gagcgagtcc gagcgcgggc gcgccctggt gttcgtccgc
     1021 accaagcgcg gcgccgaccg gctggtcaag aagctcgacc gcgagaaggt ccgcgcggtg
     1081 gccatgcacg gcaacaagtc gcaggcccag cgccagaagg cgctcgcggc gttcgagaag
     1141 ggcaccgtcg acacgctggt ggccaccgac gtggccgcac gcggcatcga cgtccctgac
     1201 atcacgcacg tgatcaactt cgacgccccg gacgatcgcg acacctacac gcaccgcgtg
     1261 ggccgcaccg gccgcgccgg caacaccggc gccggggtca gcttcgtgct gggcgaccag
     1321 gtgcacgaca tgcgcaagat cgcccgcgac ctcggtctgg gcgatcagtt cgacgccgcc
     1381 gacggcacgc ccgtcgcggt gccctcggac ggcccgaaca ccaaccgcac ccggccgcag
     1441 cgcggcggtg gcggcggcgg tggccgcaac ggcaaccgca gccgcggtgg tggcggcggc
     1501 cagggtggcg ggaaccgccc caaggccaag tccggcgccc agggctggtc gtccgacggc
     1561 cgcggcccca agcgccaggg gcgccgctcc cgctagccgg gcgcgccggc tggcgcgtcc
     1621 atagtcgcgc tcataggatg gcccgctatg tcccaggcgg ccctcctgga gcgcgaccgc
     1681 gagctcgcga ccctcgaacc cgtggtcgac gaggccgcct ccggcgaagg ccgggtggtg
     1741 gtgttcgagg ggcccgcggg catcggcaag acccggctgc tgggcgagac ccggcggctg
     1801 gcggaggccg cgggcatgcg tgtcctgtcc gcccgcggcg gggcgctgga gcgcgagtac
     1861 ccgtacgggg tggtgcgcca gctgttcgag cccgtcctgg tcgatcccga ggcgcgtgag
     1921 cggctcctgg gcgagtccgc ggccctggcc cggcccgtgt tcgagggcgc cgacgccggc
     1981 gcgccggact cgggcgagtt ctcgaccctg cacgggctct actggctgac ggtcaacctg
     2041 gccaccgagc ggccgctgat gctggcgctg gacgacctgc agtggtgcga ccggccctcg
     2101 ctgcgcttcg tggcctacct gaaccgccgt atcgagggca tgggcctgct ggtcgcggcc
     2161 acgcgccgcg acgccgagcc cggcaccgac ccggccctgc tggccgagat caccggcgac
     2221 ccgtcggcca cctcgctgcg cccgcagccg ctgagcccca ccgcggggcg cgagctggtc
     2281 agcgccgggc tgggccgcga gcccgatgag cgcttcacgg ccgcgtgcca gaaggccaca
     2341 gggggcaacc cgctgctgct gggccagctg gtctcggcgc tgcggt
//
LOCUS       DASUPW010000116         3335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_443239, whole genome shotgun sequence.
ACCESSION   DASUPW010000116 DASUPW010000000
VERSION     DASUPW010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443239"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..656)
                     /locus_tag="VFY44_02645"
     CDS             complement(138..656)
                     /locus_tag="VFY44_02645"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX5909363.1"
                     /translation="MILASASPRRRAILDQLGIAFEVVVPEVEELAEGHPRDLVLENA
                     RRKAAAVEGEDVLGADTTVALDGAVLGKPADRAEARSHLQRLSGGTHEVWSAVAFRGE
                     AVADVTSVTFRDLGPLLDWYVDTGEWEGKAGAYAIQGLGAALVERIEGDYNCVVGLPV
                     AALLALAPDLIG"
     gene            complement(653..1057)
                     /gene="ndk"
                     /locus_tag="VFY44_02650"
     CDS             complement(653..1057)
                     /gene="ndk"
                     /locus_tag="VFY44_02650"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002515614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX5909364.1"
                     /translation="MARTLILVKPDAFERNLTGEILARFERKGLKIAALKHMTVERSQ
                     AEEHYAEHKDKPFFGDLVDFITGGPLVAAVLEGHEAVKAARQVIGATNPVEAAPGSIR
                     GDFGLEVQTNLVHGSDSDESATREAGIFFPEL"
     gene            complement(1079..1795)
                     /locus_tag="VFY44_02655"
     CDS             complement(1079..1795)
                     /locus_tag="VFY44_02655"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX5909365.1"
                     /translation="MGPLAVFGITNDGLNLAVNLLLLFLVVMYFALVWWTYSDAQRRI
                     EDPMIVACATAASLFPFVGTVVYLIVRPPEYLEDVRERELEIAAAEARLAGVEHQTCP
                     YCNYEIEKSFLVCPSCMHRLKEPCTTCGKPLDPRWKVCPYCEAEVGAPAQQQPRRERR
                     PRRERPAAATGESPTQVAGAEPPPRRATKQRPPREDGQAQPRREREAAPQQRQSRPAA
                     EGPADGQQPQRERPTRPARS"
     gene            complement(2011..3267)
                     /locus_tag="VFY44_02660"
     CDS             complement(2011..3267)
                     /locus_tag="VFY44_02660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX5909366.1"
                     /translation="MSASWTLARAEEYLLGLELFGMRFGLERMHRLTTTLGMPQRRFA
                     SVHVVGSNGKSSTVRFTAAVLERHGLRTGSYTSPHLASVRERIEVGEQPVSGERFAAA
                     VERAAKAAEIVERAQAEDEDPVTQFEALTASAYHELAASKVEVAVIEAGLGGRYDATN
                     VIPSKVAVLTGVGLEHTRWLGPTVADIAEEKLAVVRDHSALVTGPLEPSARSVAERVA
                     GERHAHWVESPALVDVPLRAAGAFQRQNFAIACAAAEAFLGRALDPAAVADAAAATRI
                     PGRLDPVSERPLVLHDGAHNPAGAHALAQALPEVLADRRPRVLVASVLEDKDAAAMLE
                     ALLGQFDQVVFTRCSNPRSLSPATLESLAGRMGGPPARTVPAPADAVELARSLAGPDG
                     AVVVTGSIYLIADLVRPDASAAASTM"
BASE COUNT          448 a         1217 c         1227 g          443 t
ORIGIN      
        1 ctgtgaggta agagaagaat cccatgggtg gaggtattcg gggccgcccg cggaccgttc
       61 cacagggcgc tcccgaagga cgccagtgta gcggcaatgg acccccgtca agggggattg
      121 caaggcgcct gggcggtcta gccgatcagg tcgggggcca gcgccaggag cgcggccacc
      181 ggcaggccga ccacgcagtt gtagtcgccc tcgatccgtt ccaccagcgc ggcgccgagg
      241 ccctggatgg cgtaggcgcc cgccttgccc tcccactccc cggtgtccac gtaccagtcc
      301 agcagcgggc ccagatcgcg gaaggtgacg gaggtcacgt cggccacggc ctccccgcgg
      361 aaggcgacgg ccgaccacac ctcgtgggtg ccgcccgaga ggcgctgcag gtggctgcgc
      421 gcctccgcgc gatcggcggg cttgcccagg accgccccgt ccagggccac cgtggtgtcg
      481 gcgcccagga cgtcctcgcc ctccacggcg gccgccttgc ggcgcgcgtt ctccaggacc
      541 aggtcgcgcg gatggccctc ggccagctcc tcgacctcgg gcacgaccac ctcgaaggcg
      601 atccccagct ggtccaggat cgcccggcgc cgcggcgacg ccgaggccag gatcacagct
      661 cggggaagaa gatcccggcc tcgcgggtgg ccgactcgtc cgagtcggat ccgtgcacca
      721 ggttcgtctg gacctcgagc ccgaagtcac cgcggatcga cccgggcgcc gcctcgaccg
      781 ggttcgtggc gccgatgacc tgccgggcgg ccttgaccgc ctcgtggccc tcgagcacgg
      841 cggccaccag cggcccgccg gtgatgaagt cgaccaggtc gccgaagaac ggcttgtcct
      901 tgtgctcggc gtagtgctcc tcggcctgcg agcgctcgac cgtcatgtgc ttgagcgccg
      961 cgatcttcag gcccttgcgc tcgaagcggg ccaggatctc gcccgtcagg ttgcgctcga
     1021 aggcgtccgg cttgaccagg atcagggtcc gtgccatggg tgcttcctct cgtggtagtc
     1081 aggaacgcgc gggccgcgtg gggcgctcgc gctggggctg ctgtccgtcc gccgggccct
     1141 cggccgcggg gcgggactga cgctgttgcg gtgctgcctc gcgctcacgg cgaggctggg
     1201 cctggccgtc ctcgcgcggg ggacgttgct tggtggcccg gcgcggcggc ggctcggcgc
     1261 ccgcgacctg cgtgggcgac tcgccggtcg cggccgccgg gcgctcgcgg cgggggcggc
     1321 gctcacggcg gggctgctgc tgggcgggag cgcccacctc ggcctcgcag taggggcaca
     1381 ccttccagcg cgggtccagc ggcttgccgc aggtggtgca cggctccttg agccggtgca
     1441 tgcagctggg gcagaccagg aacgacttct cgatctcgta gttgcagtac gggcaggtct
     1501 ggtgctcgac gccggccagg cgcgcctcgg cggcggcgat ctccagctcg cgctcgcgca
     1561 cgtcctccag gtactcgggc ggccgcacga tcaggtagac gaccgttccc acgaacggga
     1621 acagcgacgc cgcggtggcg caggccacga tcatggggtc ttcgatccgg cgctgggcgt
     1681 ccgagtaggt ccaccagacc agcgcgaaat acatgaccac caggaacagc agcaacaggt
     1741 tgaccgcgag gttcaacccg tcgttcgtga tgccgaagac ggcgaggggg cccattgagc
     1801 gtggggagtc tagcggcgcg agcggcgggt actgccgccc gccgtactag aggaacacgt
     1861 tccccaggag gtagccgatc ccgaagaaca cgaggatgac ggtcgccacc accagggcca
     1921 ccaggccgat catcacgccg aagcgtggtc cgtcgccgcg ttccattcgc ggaacgatgc
     1981 cacgggtcgt gcgttctcgc cagaatcgcg ctacatcgtg gacgcggccg cgctcgcgtc
     2041 ggggcggacc aggtcggcga tcaggtagat cgagccggtg accacgacgg ccccgtcggg
     2101 cccggccagc gagcgggcca gctcgacggc gtcggcgggc gccggcacgg tccgcgcggg
     2161 cggcccgccc atccgccccg ccagcgactc gagcgtggcc ggcgacagcg agcgggggtt
     2221 cgagcagcgg gtgaacacca cctggtcgaa ctggcccagg agcgcttcca gcatggccgc
     2281 ggcgtccttg tcctcgagca cgctggccac caggacgcgc gggcggcggt cggccaggac
     2341 ctccggcagc gcctgcgcca gcgcgtgcgc gccggccggg ttgtgggcgc cgtcgtggag
     2401 caccagcggc cgctcggaca cggggtccag gcgccccggt atccgggtcg cggcggcggc
     2461 gtcggccacg gccgcggggt ccagcgcgcg gcccaggaag gcctcggcgg cggcgcaggc
     2521 gatcgcgaag ttctggcgct gaaacgctcc cgccgcgcgc agcgggacgt cgaccagggc
     2581 gggcgactcg acccagtgcg catggcgttc gccggccacc cgctcggcca ccgaccgcgc
     2641 gctgggctcc agcggcccgg tgaccagcgc ggagtggtcc ctgaccaccg ccagcttctc
     2701 ctccgcgatg tccgcgacgg tgggccccag ccagcgggtg tgctccagcc ccaccccggt
     2761 cagcaccgcg accttcgacg ggatgacgtt ggtggcgtcg tagcgcccgc ccaggcccgc
     2821 ctcgatcacg gccacctcca ccttgctggc ggccagctcg tggtaggccg aggcggtcag
     2881 cgcctcgaac tgggtcaccg ggtcctcgtc ctcggcctgc gcgcgctcga cgatctccgc
     2941 cgccttggcg gcccgctcga cggccgcggc gaagcgctcg ccggacaccg gctgctcgcc
     3001 gacctcgatc cgctcgcgga ccgacgccag gtgcggcgag gtgtagctgc ccgtgcgcag
     3061 gccatggcgc tccagcaccg cggcggtgaa gcgcacggtg gacgacttcc cgttcgagcc
     3121 caccacgtgg accgaggcga accggcgctg gggcatcccc agcgtggtgg tcagccggtg
     3181 catgcgctcc aggccgaagc gcatcccgaa cagctccagg cccagcaggt actcctcggc
     3241 ccgggccagg gtccaggagg ccgacatcag aggcgttcca gggccgccgt gtactcggcc
     3301 agcttggcgc gctcgccctc gaccacgtcg gcggg
//
LOCUS       DASUPW010000117         3597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_996596, whole genome shotgun sequence.
ACCESSION   DASUPW010000117 DASUPW010000000
VERSION     DASUPW010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996596"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="VFY44_02665"
     CDS             <1..539
                     /locus_tag="VFY44_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909367.1"
                     /translation="MWRSLSVASGDVVAFIDADTRDFDPRFVNGVVGPLLTDPSLRFV
                     KATYRRPFTAGGLEVPGGGGRVSQLTARPLLDAFYPDLAALTQPLAGEVAATRELLES
                     IPFATGYAVETAMLLDVHEAVGSAAMAQCDVGERRNHHQPLDALRPMAETVLAVVCEH
                     QGLDTAGPEIVRRPPFSR"
     gene            536..1291
                     /locus_tag="VFY44_02670"
     CDS             536..1291
                     /locus_tag="VFY44_02670"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX5909368.1"
                     /translation="MSLRCVYTDLDGTLLGAGASLFRDGEGAFTLLPARALEACHRAG
                     VEVVLKSGRRKAQVMEDARLIGQASYIYEVGSGLVIDGEEQLLCGEFTPTGDETPHEL
                     IAATGAPALLERELDIQPHAPWHVDRFVSHLYRGSADVGRANELLEAEGHGGLRLIDN
                     GAAAEGGHTYHLIPATASKAAAVERHMRARGLAPEECIAVGDSEEDLGVAAVVGRFFL
                     VANGPDLSGPNVTRTESGHGEGFYEAVVSSLVG"
     gene            1299..2948
                     /locus_tag="VFY44_02675"
     CDS             1299..2948
                     /locus_tag="VFY44_02675"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5909369.1"
                     /translation="MDTGSAGPQHSEGLRLLEAAQWAAARDAFQATLDERETPDAHDG
                     IGLALFFLGQIEDGIAARERAFEGYVAADRCDEAARVGVWVSHQYLLSGRASAARGWL
                     ARVERAVQDPRCEGQGWVAVERARHAETLDQQAAHSRRALAIARESGNGDLETYALSL
                     MGLTEVSAGHLEPGMQLLEEAMAAASAGRVRNVHTLAEAYCNLIVASTDAGDWDRASE
                     WCDLVDEFARERGAMPLVGACRTIHADVLVARGRWPEAEVALQAALETHARYIPAMGA
                     PTVASMAELRVRQGRLAEAEQLLTGREEHPSSLCALAQLRIADGHPQVAAGLLERALP
                     AAEGNAIRTTQLLAPLVEARLACGDPQGARAATSQLAALADESGIRLVGARAELAAAH
                     VTLAAGRPGEAADPARRALAAFGRLGMPLHVGEARLALARSLVLDSPDVARDEARAAF
                     DAFRQLGASRAMDAAAGVLRELGAATGARPRSQGELTAREREVLELVARGMSNAQIAQ
                     TLVITEKTAGHHVSRILSKLGVRNRTEAAAHAAHREQTGTR"
     gene            3018..>3597
                     /locus_tag="VFY44_02680"
     CDS             3018..>3597
                     /locus_tag="VFY44_02680"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5909370.1"
                     /translation="MSVDTADSELKARHRKMWASGDYPSMVETFLLPLGPRLVDACAI
                     QPGDRVLDVAAGTGNAAIRAAQRGATVTASDLTPELLTAGRQVAEGDGIELDWVEADA
                     EHLPFEDGSFDVVMSSIGAMFAPHHQAVADELVRVCRPGGKVGLLSWTPEGMLGALFR
                     TIGPFAPAPPPGAQPPPLWGSEEHLRGLFGGRV"
BASE COUNT          443 a         1322 c         1381 g          451 t
ORIGIN      
        1 ccatgtggcg gtcgctctcg gtggcgtccg gagacgtggt ggcgttcatc gacgccgaca
       61 cccgcgactt cgacccgcgt ttcgtgaacg gcgtggtggg cccgctgctg accgacccgt
      121 cgttgcggtt cgtcaaggcc acctaccggc gcccgttcac ggcgggcggg ctcgaggtac
      181 ccggtggcgg cggccgggtc agccagctga ccgcgcgccc gctgctggat gcgttctacc
      241 ccgacctggc cgccctgacg cagccgctcg cgggcgaggt ggccgccacg cgcgagctgc
      301 tggagtcgat cccgttcgcc accggctacg cggtggagac ggcgatgctg ctggatgtcc
      361 acgaggcggt gggctccgcc gcgatggcgc agtgcgacgt gggcgagcgg cgcaaccacc
      421 accagccgct ggacgccctg cgcccgatgg ccgagaccgt cctggccgtg gtgtgcgagc
      481 accagggtct ggacacggcc gggcccgaga tcgtccggcg gccacccttc tcccgatgag
      541 cctgcgctgc gtgtacaccg acctggacgg caccctgctg ggggccggcg cgtcgctgtt
      601 ccgcgacggc gagggcgcct tcaccctgct gcccgccagg gcgctggagg cctgccaccg
      661 cgccggtgtc gaggtagtgc tcaagtcggg ccggcgcaag gcccaggtca tggaggacgc
      721 gcgcctgatc ggccaggcct cgtacatcta cgaggtcggc tccgggctgg tcatcgacgg
      781 tgaggagcag ctgctctgcg gcgagttcac gcccaccggg gacgagaccc cgcacgagct
      841 gatcgccgcc acgggcgcgc ccgcgctgct ggagcgcgag ctggacatcc agccccacgc
      901 gccctggcac gtggaccggt tcgtctccca tctgtaccgg ggcagcgccg acgtggggcg
      961 ggccaacgag ctcctggagg ccgagggtca cggcggcctg cggctgatcg acaacggcgc
     1021 ggcggccgag ggcggtcaca cctaccacct gatccccgcc acggcgtcca aggcggcggc
     1081 cgtcgagcgc cacatgcgcg cccgcgggct cgcgcccgag gagtgcatcg cggtcggcga
     1141 ctccgaggag gacctgggcg tcgcggcggt ggtggggcgg ttcttcctgg tcgcgaacgg
     1201 ccccgacctg tccgggccca acgtcacccg caccgagtcg ggccacggcg agggcttcta
     1261 cgaggccgtg gtctcgtccc tggtgggata atcgcgccat ggacaccggg agcgctggcc
     1321 cacagcactc cgagggactg cgcctcctgg aggccgcgca gtgggctgcg gcccgcgatg
     1381 ccttccaggc cacgctggac gagcgcgaga cgccggacgc ccacgacggc atcgggctgg
     1441 cgctgttctt cctggggcag atcgaggacg ggatcgccgc gcgcgagcgc gcgttcgagg
     1501 ggtacgtggc ggccgaccgg tgcgacgagg ccgcgcgggt gggcgtctgg gtctcgcacc
     1561 agtacctgct ctcggggcgc gcctcggcgg ctcgtggctg gctggcccgc gtggagcgcg
     1621 cggtccagga cccacggtgc gagggccagg gatgggtggc ggtcgagcgt gcgcggcacg
     1681 ccgagaccct ggaccagcag gcggcccact cgcgccgggc cctggcgatc gcgcgcgagt
     1741 ccggcaacgg cgacctggag acctacgcgc tcagcctgat ggggctgacc gaggtcagcg
     1801 cggggcacct cgagcccggg atgcagctgc tcgaggaggc catggcggcg gcctcggcgg
     1861 gccgggtgcg caacgtgcac acgctggccg aggcctactg caacctgatc gtggcgtcca
     1921 ccgacgccgg cgactgggac cgcgcctcgg agtggtgcga cctggtcgac gagttcgcgc
     1981 gcgagcgggg cgcgatgccc ctggtcggcg cctgtcgcac gatccacgcc gacgtgctcg
     2041 tggcccgcgg gcgctggccc gaggccgagg tcgcgctcca ggccgcgctc gagacccacg
     2101 cccgctacat cccggcgatg ggagcgccga cggtggccag catggcggag ctccgcgtgc
     2161 gccagggccg cctggccgag gccgagcagc tgctgacggg ccgcgaggag cacccgtcct
     2221 cgctctgcgc cctcgcgcag ctgcggatcg ccgacgggca tccacaggtc gccgcggggc
     2281 tcctggagcg cgccctcccc gccgccgagg gcaacgccat ccggaccacg cagctgctgg
     2341 ccccgctggt cgaggcccgc ctggcgtgcg gcgatcccca gggtgcccgg gccgccacgt
     2401 cgcagctggc cgcactcgcg gacgagtccg gcatccggct ggtcggcgcc cgcgcggagc
     2461 tggccgccgc gcacgtgacc ctggccgcgg gccggcccgg cgaggcggcg gacccggccc
     2521 gccgcgcgct ggccgccttc ggccggctgg ggatgccgct gcacgtcggc gaggcacggc
     2581 tggcgctggc ccggtccctg gtgctggact cacccgacgt ggcccgtgac gaggcgcggg
     2641 cggcgttcga cgcgttccgc cagctggggg cctcgcgggc catggacgcc gccgccggcg
     2701 tgctgcgcga gctgggcgcg gccaccggcg cccggccccg cagccagggc gagctcacgg
     2761 cacgcgagcg cgaggtgctg gagctggtgg cgcgtggcat gtccaacgcc cagatcgcgc
     2821 agacgctggt gatcaccgag aagaccgccg gccaccacgt cagccggatc ctctccaagc
     2881 tcggtgtgcg caaccgcacc gaggcggcgg cccacgcggc gcaccgggag cagacgggca
     2941 cgagataggg attcctcccg atgtatggcc gccggagccc gcctagcgtg ctcgcatacc
     3001 ggcgatcagg aggaaccatg tccgtagaca ccgcagattc ggagctcaag gcccgccacc
     3061 gcaagatgtg ggcctcgggc gactacccgt cgatggtcga gacgttcctg ttgccgctcg
     3121 ggccgcggct ggtggacgcg tgcgcgatcc agcccgggga ccgcgtcctg gacgtcgcgg
     3181 ccggcaccgg caacgccgcc atccgcgcgg cgcagcgggg cgcgacggtc acggccagcg
     3241 acctgacccc cgagctgctc acggccggcc gccaggtggc cgagggggac ggcatcgagc
     3301 tggactgggt cgaggccgac gccgagcacc tcccgttcga ggacgggtcg ttcgacgtgg
     3361 tcatgtcctc gatcggcgcg atgttcgcgc cccatcacca ggccgtcgcc gacgagctgg
     3421 tgcgggtctg ccgccccgga gggaaggtgg gcctgctgag ctggacgccg gagggcatgc
     3481 tcggcgcgct gttccgcacc atcgggccgt tcgcgcccgc gccgcccccc ggcgcgcagc
     3541 ccccgcccct gtggggcagc gaggagcacc tgcgcggcct gttcggcggc cgcgtgg
//
LOCUS       DASUPW010000118         2696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_165640, whole genome shotgun sequence.
ACCESSION   DASUPW010000118 DASUPW010000000
VERSION     DASUPW010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165640"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VFY44_02685"
     CDS             <1..775
                     /locus_tag="VFY44_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HEX5909371.1"
                     /translation="RLADFGRWQDAMLAGEQLMWHSHISAALNLGLLDPMECVEAALA
                     VEDAPLNAVEGFVRQVIGWREYIWGTYWLFGDRWDSDNALDADTDLPAAFWDAGTDMR
                     CVSESVEGLRATGYAHHIQRLMVFGNLAMLLGVEPRQVYDWFHESYIDGYPWVMAPNV
                     LAMATWADGGRMFTKPYAAGGRYIDRMSDYCGECRFDPKKRTGDDACPFTTLYWDFLD
                     RNRPRLAGIHRLNMPLRNLDGIDADELREIRRRARRIRA"
     gene            complement(838..1212)
                     /locus_tag="VFY44_02690"
     CDS             complement(838..1212)
                     /locus_tag="VFY44_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909372.1"
                     /translation="MSSSRLISADMRSALLIAVGVAIPVTAALLSLSEAAQVAGLAIA
                     ILVMGLGLAGTASSGRGTLPVSQQSGYDLGLAVGLAGAAVLFALVGDAPAVALFGGFA
                     ALTMLINLLTRYTPARRTENFL"
     gene            1286..2077
                     /locus_tag="VFY44_02695"
     CDS             1286..2077
                     /locus_tag="VFY44_02695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX5909373.1"
                     /translation="MAKRRLDALLADRGLYESRSRAAAAVIAGEVRLAPGGQIADKPG
                     RMVADDVGVQVAERPQYVSRGGVKLANALDAFGLDVAGRRALDAGASTGGFTDCLLQR
                     GAEAVVAADVAYGELHWSMRQDERVTVLERTNVKELDPAALPWPPDLIVADLSFISLH
                     KVLPALMGAATPVFDCLVMIKPQFEVGRQKVGKGGVVRDPALRREALVSVGAAALRLG
                     ASVMGYASSGLPGPKGNRESFVWLAEAGRPGALHDLDQAVLRAEP"
     gene            2074..>2696
                     /locus_tag="VFY44_02700"
     CDS             2074..>2696
                     /locus_tag="VFY44_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX5909374.1"
                     /translation="MTAPKSVAVFTRRNPEKTVAAVHRLAELAAEAGVELLVPSEEVD
                     KHGLQDLPGATFDADPLTADLALVFGGDGTILTTMRRFAGRDVPVFAVNFGAVGFLST
                     VESGDLDAALGSALRGEYEVLPLPVLSADVNGAPQVAVNDISLHRRQDLRVAELAFSV
                     AGQELGQVRCDGLVVATPAGSTGYNLANGGPVLAWGVEGFVVSFIAP"
BASE COUNT          375 a          930 c         1017 g          374 t
ORIGIN      
        1 gcggctggcc gacttcgggc gctggcagga cgccatgctg gccggcgagc agctgatgtg
       61 gcactcgcac atcagcgcgg cgctcaatct cggcctgctg gacccgatgg agtgcgtgga
      121 ggccgcgctg gcggtggagg acgccccgct caacgcggtc gagggcttcg tgcggcaggt
      181 gatcggctgg cgcgagtaca tctggggcac ctactggctg ttcggcgacc ggtgggactc
      241 cgacaacgcg ctggacgccg acaccgacct cccggcggcg ttctgggacg ccggcaccga
      301 catgcgctgc gtgtcggagt cggtggaggg gctgagggcg accggctacg cccaccacat
      361 ccagcggctg atggtcttcg gcaacctggc gatgctcctg ggcgtggagc cgcgccaggt
      421 ctacgactgg ttccacgaga gctacatcga cggctatccc tgggtcatgg cgcccaacgt
      481 gctggccatg gccacgtggg ccgacggtgg ccgcatgttc accaagccct acgccgccgg
      541 cgggcgctac atcgaccgca tgtccgacta ctgcggggaa tgccgcttcg accccaagaa
      601 gcggaccggc gacgacgcgt gcccgttcac gacgctctac tgggacttcc tggaccgcaa
      661 ccggccccgg ctcgcgggca tccaccgcct gaacatgccg ctccgcaacc tggacggcat
      721 cgacgcggac gagctgcggg agatccgccg gcgagcccgc cggatccggg cctagaaaag
      781 gaaacgaccc gccgaagcgg gccgtttccg agggaggagt gatgcgatat ggatctgtta
      841 gaggaagttc tcggtgcggc gtgccggcgt gtaccgggtc aacagattga tcagcatcgt
      901 cagcgctgcg aagccgccga acagggcgac cgcgggcgcg tcgccgacca gggcgaacaa
      961 caccgcggcg cccgcgagtc cgacggccag tcccaggtca tagcccgact gctggctcac
     1021 ggggagggtg cctcggccag atgaggcggt gcccgcgagc ccgaggccca tgaccaggat
     1081 ggcgattgcg agtccggcca cctgagccgc ttcgctgagc gagagcagcg ctgcggtgac
     1141 cgggattgcc acgccgacgg cgatcagcag tgctgagcgc atgtcggcgg atatgaggcg
     1201 ggaactgctc acggatccaa cgtactccgg gcccgggagg ccactgtggg ggtggtcaca
     1261 gagccccggc gggtaccctc gccgggtggc gaagcggcgt ctggacgcct tgttggccga
     1321 ccgcggcctg tatgaatctc ggtcgcgcgc ggcggccgcg gtgatcgccg gggaagtccg
     1381 gctggccccg ggcggccaga tcgccgacaa gccggggcgg atggtggccg acgacgtcgg
     1441 cgtccaggtg gccgagcggc cccagtacgt ctcgcgcggg ggcgtgaagc tggccaacgc
     1501 gctcgacgcc ttcgggctgg acgtggcggg ccgccgtgcc ctggacgcgg gcgcctccac
     1561 cggcggcttc accgactgcc tgctccagcg cggcgccgag gcggtcgtgg cggccgacgt
     1621 ggcctacggc gagctccact ggagcatgcg ccaggacgag cgcgtcaccg tgctcgagcg
     1681 caccaacgtc aaggagctgg acccggccgc gctgccgtgg ccgcccgacc tgatcgtggc
     1741 cgacctgtcc ttcatctcgc tgcacaaggt gctgccggcg ctgatgggcg ccgccacgcc
     1801 ggtgttcgac tgcctggtga tgatcaagcc ccagttcgag gtcggccggc agaaggtcgg
     1861 caagggcggc gtggtgcgcg accccgccct gcgccgcgag gcgctggtgt cggtcggtgc
     1921 cgcggccctg cggctgggcg cctcggtcat gggctacgcc tcgtcggggc tgccgggccc
     1981 gaaggggaac cgcgagagct tcgtctggct ggccgaggcg ggacgcccgg gcgccctcca
     2041 cgacctcgac caggccgtcc tgagggccga gccgtgaccg cgccgaagtc ggtggcggtg
     2101 ttcacccgcc gcaacccgga gaagacggtc gcggccgtgc accggctggc cgagctggcg
     2161 gccgaggcgg gcgtcgagct gctcgtgccc agcgaggagg tcgacaagca cggcctgcag
     2221 gacctgcccg gcgcgacctt cgacgccgac ccgctgaccg ccgacctggc gctggtgttc
     2281 ggcggcgacg ggacgatcct gaccaccatg cgccgcttcg ccggccgcga cgtgccggtg
     2341 ttcgccgtca acttcggcgc cgtcggcttc ctgtccacgg tcgagagcgg cgacctggac
     2401 gcggcgctgg gcagcgcgct gcggggcgag tacgaggtgc tgccgctgcc ggtgctgagc
     2461 gccgacgtga acggcgcgcc gcaggtggcg gtcaacgaca tctcgctgca ccggcgccag
     2521 gacctgcgcg tggccgagct ggccttctcc gtggcgggcc aggagctggg ccaggtgcgc
     2581 tgcgacggcc tggtggtggc cacccccgcc ggctccacgg gctacaacct ggccaacggc
     2641 gggccggtgc tggcctgggg cgtggagggc ttcgtcgtct ccttcatcgc cccgca
//
LOCUS       DASUPW010000119         2237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1689131, whole genome shotgun sequence.
ACCESSION   DASUPW010000119 DASUPW010000000
VERSION     DASUPW010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689131"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VFY44_02705"
     CDS             <1..506
                     /locus_tag="VFY44_02705"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5909375.1"
                     /translation="CPYCHGWEHRDGRLAVLGGQGAAHRAPLLRQWSDDVIVLADDLS
                     GEDRDALDQAGIPVDERPVAGVEGDGGHLGAVRFADGSRLERDGVMVVAPLRPRDDLG
                     PRLGCETTDTPIATGLLVTDNLQQTTVPGVFAAGDVSAMMQQVALAIAAGSLAAAATH
                     RSLIFGL"
     gene            503..1480
                     /locus_tag="VFY44_02710"
     CDS             503..1480
                     /locus_tag="VFY44_02710"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5909376.1"
                     /translation="MTPLLHQRHPELAATLPHVSLGTTPTPVRRLEALEPAGTELWIK
                     DDGAFGDGGWGGNKVRKLEWILPEARRRGARTILTVGGLGTNWGLATALYGRDHGVAT
                     ALLLVDQPVDDHVVAQQERLKASGATLHYTHTKARTVASLPLLMARHSAGGRPPYLQP
                     AGGSSAVGALGYVEAAYEIAGQVEAGELPAPTHVVTTVGSGGTAAGLALGLALAGLDA
                     TVIGIVVNDGLRLDTPALTRLAGRTRRLLARRGAAVGDPRLRLQSLDGWMGAGYGHPT
                     AEATEAIADAAASEALTLDPVYTSKTMSAIRAMDLGPGPVLYIHTDGPR"
     gene            1485..>2237
                     /locus_tag="VFY44_02715"
     CDS             1485..>2237
                     /locus_tag="VFY44_02715"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5909377.1"
                     /translation="MEQLDWGEGNYARTAEAIAPASEALVEFARIEAGQAVLDVACGT
                     GNAAIAAAERGATVTGVDPAEVLIEQAAARAAERGIDARWIRGGADELPVADGAFDVA
                     MSVFGVIFAPDPGAAAAEMVRAVRPGGTVALTSWRPSGPISAVGGIMFRALPSADGPP
                     RDWGDPGWTTATLEAAGAKDVRIEETELAFTAASPQAWLDEQCANHPVWRWARRQLGE
                     EKWREVEAESVAALTEGNEDPDAFRTNSRYLLV"
BASE COUNT          288 a          818 c          876 g          255 t
ORIGIN      
        1 actgccccta ctgccacggc tgggagcacc gcgacggccg cctggcggtg ctgggcggcc
       61 agggcgccgc gcaccgcgcg ccgctgctga ggcagtggtc cgacgacgtg atcgtcctgg
      121 ccgacgacct gtccggcgag gaccgcgacg cgctggacca ggcggggatc cccgtcgacg
      181 agcgcccggt ggccggggtc gagggcgacg gcgggcacct gggcgccgtg cggttcgccg
      241 acggcagccg gctcgagcgc gacggcgtga tggtggtggc tccgttgcgc ccgcgggacg
      301 acctgggccc acggctgggg tgcgagacca ccgacacgcc gatcgccacc ggcctcctgg
      361 tcaccgacaa cctgcagcag accaccgtgc ccggcgtgtt cgcggcgggt gacgtttcgg
      421 cgatgatgca gcaggtggcg ctggccatcg ccgcgggatc gctggcggcg gccgcgacgc
      481 accgttcgct catattcggt ctgtgacacc cctcctccac cagcgccacc ccgagctcgc
      541 cgccaccctc ccccacgtgt cgctgggcac gacgccgacg ccggtgcggc ggctcgaggc
      601 gctggagccc gcgggcaccg agctctggat caaggacgac ggcgcgttcg gcgacggcgg
      661 ctggggcggc aacaaggtgc gcaagctcga gtggatcctg cccgaggcac gccggcgcgg
      721 cgcgcgcacc atcctgaccg tcggcgggct gggcaccaac tggggcctgg ccaccgccct
      781 ctacggccgc gaccacggcg tggcgaccgc gctgctgctg gtcgaccagc cggtcgacga
      841 ccacgtggtg gcccagcagg agcggctgaa ggccagcggc gcgacgctgc actacacgca
      901 caccaaggcg cggaccgtcg cctcgctccc gctgctgatg gcccgccaca gcgccggcgg
      961 ccggccgccc tacctgcagc cggccggcgg gtcgtccgcg gtcggcgcgc tgggctacgt
     1021 ggaggcggcg tacgagatcg ccggccaggt cgaggcgggc gagctgcccg cccccaccca
     1081 cgtggtgacc acggtcggct ccggtggcac ggccgccggg ctggccctgg gccttgcgct
     1141 ggccggcctg gacgcgaccg tgatcggcat cgtggtcaac gacgggctgc ggctggacac
     1201 cccggccctg acccgcctgg ccgggcgcac ccggaggctg ctggccaggc gcggcgccgc
     1261 ggtcggcgac ccgcggctgc ggctccagag cctggacggc tggatgggcg cgggctacgg
     1321 ccaccccacc gcggaggcga cggaggcgat cgccgacgcc gcggcttccg aggcgctcac
     1381 gctcgatccg gtgtacacct ccaagaccat gagcgcgatc agggcgatgg acctgggccc
     1441 cggacccgtc ctgtacatcc acacggacgg gccgaggtag gccgatggag cagctggact
     1501 ggggcgaggg caactacgcg cgcaccgcgg aggcgatcgc gccggcgtcc gaggcgctgg
     1561 tggagttcgc caggatcgaa gcggggcagg cggtgctgga cgtggcctgc ggcaccggca
     1621 acgcggcgat cgcggcggcc gagcggggcg ccacggtcac cggcgtggac ccggcggagg
     1681 tgttgatcga gcaggcggcg gcccgggctg ccgaacgcgg catcgacgcc cgctggatcc
     1741 gcggcggcgc cgacgagctg ccggtggccg acggcgcctt cgacgtcgct atgagcgtgt
     1801 tcggcgtgat cttcgccccc gaccccggcg cggccgccgc ggagatggtg cgcgccgtcc
     1861 gcccgggagg gacggtggcg ctgacgtcgt ggaggccgtc gggccccatc agcgccgtcg
     1921 gcgggatcat gttcagggcg ctgccgtcgg cggacggccc gccgcgcgac tggggcgatc
     1981 ccggctggac caccgccacg ctggaggcgg ccggcgcgaa ggacgtgcgc atcgaggaga
     2041 ccgagctcgc cttcaccgcc gcgtcacccc aggcgtggct ggacgagcag tgcgccaacc
     2101 acccggtctg gcgctgggcc cggcgtcagc tgggcgagga gaagtggcgc gaggtggagg
     2161 ccgagagcgt ggccgccctg accgagggca acgaggaccc ggacgccttc cggaccaaca
     2221 gccgctacct gctggtg
//
LOCUS       DASUPW010000120         6765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_719634, whole genome shotgun sequence.
ACCESSION   DASUPW010000120 DASUPW010000000
VERSION     DASUPW010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719634"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..891
                     /locus_tag="VFY44_02720"
     CDS             142..891
                     /locus_tag="VFY44_02720"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX5909378.1"
                     /translation="MASDTQPGMTVEQLAQESGMSVRNIRNHQSRGLLPPPEVRARTG
                     YYGPEHVERLRLIQQMQAEGFNLGAIQRLIGDEGPGADRFAAIRQLIADPEAGEQPEV
                     LTADELTDRFGPIGPKVLARAQKLGVVVNLGDGTFEVPSPTLLRAADELLARGVSLST
                     ALGVLDKVRRNSQNTARAFVDLFLEEVWKPFDREGRPEDRWGEISESIEGLRPLASDA
                     VLATFRQVMAAEMEDAFGKLLREQGRGKGKG"
     gene            947..1336
                     /locus_tag="VFY44_02725"
     CDS             947..1336
                     /locus_tag="VFY44_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909379.1"
                     /translation="MRRLPLLIICLAALLFAAVPASAKDGGDDKDGRVIKRGDCSGAS
                     NWKLKAKPDDGGLEVEFEVDQNRNGVKWNVALRRNGKTVSRGSRTTRAPSGSFEFERD
                     IANPRGTDTISAVATRAGETCRASLKI"
     gene            1398..1544
                     /locus_tag="VFY44_02730"
     CDS             1398..1544
                     /locus_tag="VFY44_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909380.1"
                     /translation="MERGIEILIGAIALIAVVCCLLVAGGQPGSAWGALPGDQKAEAA
                     PPTR"
     gene            1603..3003
                     /locus_tag="VFY44_02735"
     CDS             1603..3003
                     /locus_tag="VFY44_02735"
                     /inference="COORDINATES: protein motif:HMM:NF017505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HEX5909381.1"
                     /translation="MALLKPDPSFYASPGEAVQAPPETLAYVATLNTGTNGDSAPDAL
                     NVLDLEQGSSTYGQLVGRVDMPEVGDELHHFGWNACSSALCPWAAHPHVERRYLLVPG
                     LRSSRIHVIDTKADKHRPEIVKVIEAEEIAKKTGYSRPHTIHCGPDGIYVSALGNPEG
                     GGPGGIFLLDHDTFEVKGPWEKDRGPQELAYDFWWHINHNTLISSEWGTPEMVEDGIN
                     PELLLGGKYGHHLHVWDIDSRSHRQSIDLGADQQMVLELRPAHDPNKTYGFVGVVVST
                     ADLSASVFLWERGPDGSVSARKVISIPAEPAEEDQLPPALKPFGAVPPLITDIDLSVD
                     DKDLYVSCWGTGEIKRFDVSDPGNPRETGSVRLGGIVERSAHPAAGSVNGGPQMVEVS
                     RDGRRVFLTNSLYASWDAQFYPDGIDGWLTKLDAPEGGGLSVDPDVFVEFSGERPHQV
                     RLEGGDASSDSYCFPD"
     gene            3014..3664
                     /locus_tag="VFY44_02740"
     CDS             3014..3664
                     /locus_tag="VFY44_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909382.1"
                     /translation="MDGLWPWIVMAGLGAYHGLNPAMGWLFAVSLGMQDRDRSSVTRA
                     LVPIAIGHELSIALVALLVIGLGVVTDTTALHLGAAVALIAFGIFRFAKPRAHFRWVK
                     GRVDRRELSVWSFLMSTAHGAGLMVAPVLIGSSQAQASEGHSHAVQEMGLGRLSIPET
                     VLAVSVHVGAMLLVMGTVALLVYEKLGVRVLRKTWVNSDHLWAASFVAAGVITLVT"
     gene            3707..4786
                     /locus_tag="VFY44_02745"
     CDS             3707..4786
                     /locus_tag="VFY44_02745"
                     /inference="COORDINATES: protein motif:HMM:NF025700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HEX5909383.1"
                     /translation="MEAHAHHHEMPTSGTALTGVAISATLHCLTGCAIGEVAGMAIGT
                     ALGFSDWGTVALAVALAFLFGYSLTSLPLLRAGLALSVVVPIALASDTLSIATMEIVD
                     NAVMLAIPGAMHAGLGDVLFWGSLAFALAVAGVVAVPVNRWLIARGKGHAKVHETGIH
                     GGPSPRLVGIIAAAAFAFGSTVLVAEALSEDEPAHGGGHAAQGRLSERAFLTEMTAHH
                     QSAIRTGHQAQERGQSAFVRGLGADIVRTQAAEVRQMRALYRRLVGGAMPAAQMHDGV
                     QARLARSREFDRAFVDHMIPHHVDAVRMAREVRTGDRELRALAAAIVTAQRREIGQMR
                     HFRDRTHGTREAPSPADDGHGGHTD"
     gene            complement(4774..5448)
                     /locus_tag="VFY44_02750"
     CDS             complement(4774..5448)
                     /locus_tag="VFY44_02750"
                     /inference="COORDINATES: protein motif:HMM:NF022805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3159 domain-containing protein"
                     /protein_id="HEX5909384.1"
                     /translation="MTVLAMMGRLADPRDTTEEPPPPNLIEAAGGPLGMAETALPGLA
                     FVVAYTVTGQDTNLSAYVALGLAAALAAARLARRETPRHALSGLFGVALAAFVATRSG
                     KAENFFLPGLIFNAGYAAAFAISVLARYPIVGYLATQLDGTAKTAWREDRARMRVYNQ
                     ASWMWSGLFVIRLAVQLPLYLAGAVVALGIARTAMGLPLFALGLWLSYLLVRRAPPPE
                     PEAQSV"
     gene            5452..6225
                     /locus_tag="VFY44_02755"
     CDS             5452..6225
                     /locus_tag="VFY44_02755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5909385.1"
                     /translation="MEIDLSGRTAFVSGSTQGIGRAIAEKLAGAGADVTVIGRGRERV
                     DAVAAELGTRGIAADVSTAEGAALVAEQLPAVDILVNNLGIFEPIPMLEVDDDTWQRY
                     WDVNVMSAIRLTRLYAPGMADRGWGRVQFMASDSAVVIPAEMAHYGVTKTALLGVSRG
                     FAKALSGTGVTVNTVMAGPTHTEGVEQFVRELVGDDKEWGEAQAEFMAEHRPHSLIER
                     LIEPEEIANMVAYLSSDAASATTGGALRVDGGYVDYIVP"
     gene            6299..>6765
                     /locus_tag="VFY44_02760"
     CDS             6299..>6765
                     /locus_tag="VFY44_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5909386.1"
                     /translation="MRADLPPGPSYPSLVHAIGFWTRPLAWLERNRARYGKRFTTRFP
                     LSPHFVILSEPDELKQVFTAPPDVLHPGQGARILEPIVGKNSVLLLDEDAHMSQRKLM
                     LPAFHGKKMAALDGLMTEVAEREVASWPGGKPIALHPRMQRLTLEIILRAAFGL"
BASE COUNT          999 a         2408 c         2433 g          925 t
ORIGIN      
        1 ggcgccgccg gcgggctcgg tcccctgtgc gatcagtgct gtggccaggc tcaaggtgct
       61 ctccctcgtc atcgagttga cgattacttc atacaacgtt acctcgatcg aggttacata
      121 gagtcggtag cgttgccaac cgtggcatcc gacacacagc ccggcatgac cgtcgagcag
      181 ctggcgcagg agtcgggcat gtccgtgcgc aacatccgca accaccagtc gcgcggcctg
      241 ctgcccccgc ccgaggtgcg cgcgcgcacg ggctactacg ggcccgagca cgtcgagcgg
      301 ctgcgcctga tccagcagat gcaggccgag ggcttcaacc tgggcgcgat ccagcggctg
      361 atcggcgacg agggcccggg ggcggaccgg ttcgccgcca tccgccagct gatcgccgac
      421 ccggaggcgg gcgagcagcc cgaggtgctc accgccgacg agctgaccga ccgcttcggc
      481 ccgatcggcc cgaaggtgct ggcccgcgcc cagaagctgg gggtggtggt caaccttggc
      541 gacgggacct tcgaggtgcc cagccccacc ctgctgcggg cggccgacga gctgctggcc
      601 cggggggtga gcctgtccac cgcgctcggc gtgctggaca aggtgcgccg caactcccag
      661 aacacggccc gcgcgttcgt cgacctcttc ctcgaggagg tgtggaagcc gttcgaccgc
      721 gagggccggc ccgaggatcg ctggggcgag atctccgagt cgatcgaggg gctgcgcccg
      781 ctggcctcgg acgccgtgct ggccaccttc cgccaggtca tggcggccga gatggaggac
      841 gcgttcggca agctcctgcg cgagcagggc aggggcaagg gcaagggctg agtaagggtt
      901 cggttaggtc ccccgcgggg ccgccggccg gtgatatcac cgccccatgc gacgactacc
      961 actgctcatc atctgccttg ccgccctgct gttcgcggcc gtcccggcct ccgcgaagga
     1021 cggtggcgac gacaaggacg gccgcgtgat caagcgcggc gactgctcgg gcgccagcaa
     1081 ctggaagctc aaggccaagc ccgacgacgg gggcctggag gtcgagttcg aggtcgacca
     1141 gaaccgcaac ggcgtgaagt ggaacgtggc cctgcgccgc aacggcaaga ccgtctcgcg
     1201 cgggagccgc accacccggg cgcccagcgg ctcgttcgag ttcgagcgcg acatcgccaa
     1261 cccccgggga accgacacca tctccgccgt ggccacgcgc gccggcgaga cctgccgcgc
     1321 ctcgctcaaa atctaggggt gcattcgcgg gtggcccccc gatgtagggg ggccgcttcc
     1381 gcggcagtct ggggggtatg gaacgaggga tagagatcct gatcggcgcc atcgccctga
     1441 tcgccgtggt ctgctgcctg ctggtcgccg ggggacagcc cgggtcggcc tggggtgcgc
     1501 tgcccggcga ccagaaggcg gaggccgcgc cgcccacccg ttaactcgac tttccctacc
     1561 gggtgggtat acattgcagg gaccccgaca ccggagcacg ccatggccct gctcaagccc
     1621 gacccgagct tctacgcatc ccccggcgag gccgtccagg ccccgcccga gacgctggcc
     1681 tacgtggcca cgctgaacac cggcaccaac ggcgacagcg cccccgacgc cctgaacgtc
     1741 ctcgacctcg agcagggctc gtccacctac ggccagctag tcggccgggt ggacatgccg
     1801 gaggtcggcg acgagctgca ccacttcggc tggaacgcct gctcgtcggc gctgtgcccg
     1861 tgggccgccc acccgcacgt cgagcgccgc tacctgctgg tgcccggcct gcgctcctcg
     1921 cgcatccacg tgatcgacac caaggccgac aagcaccggc ccgagatcgt caaggtcatc
     1981 gaggccgagg agatcgcgaa gaagaccggc tacagccggc cgcacaccat ccactgcggc
     2041 cccgacggga tctacgtctc ggcgctcggc aaccccgagg gtggcggccc gggcgggatc
     2101 ttcctgctgg accacgacac gttcgaggtc aagggcccgt gggagaagga ccgcggcccg
     2161 caggagctgg cgtacgactt ctggtggcac atcaaccaca acacgctgat ctccagcgag
     2221 tggggcacgc ccgagatggt cgaggacggc atcaaccccg agctgctgct gggcggcaag
     2281 tacggccacc acctgcacgt gtgggacatc gacagccgct cgcaccggca gtcgatcgac
     2341 ctgggcgccg accagcagat ggtgctggag ctgcgcccgg cccacgaccc gaacaagacg
     2401 tacgggttcg tgggcgtcgt ggtgtccacc gccgacctgt ccgcgtcggt gttcctgtgg
     2461 gagcgcgggc ccgacggcag cgtcagcgcg cgcaaggtca tctcgatccc ggccgagccg
     2521 gccgaggagg accagctgcc gccggcgctc aagccgttcg gcgccgtgcc gccgctgatc
     2581 accgacatcg acctgtcggt ggacgacaag gacctgtacg tctcgtgctg gggcacgggc
     2641 gagatcaagc gcttcgacgt ctccgacccc ggcaacccgc gcgagaccgg ctcggtccgc
     2701 ctcggcggca tcgtggagcg ctccgcgcac ccggccgcgg gctcggtcaa cggcgggccg
     2761 cagatggtgg aggtcagccg cgacggccgg cgggtgttcc tgaccaactc gctctacgcc
     2821 agctgggacg cccagttcta ccccgacggc atcgacggct ggctgaccaa gctggacgcg
     2881 cccgagggcg gcggcctgag cgtggacccg gacgtgttcg tggagttcag cggcgagcgg
     2941 ccccaccagg tgcgcctgga gggcggcgac gcgtcctccg actcctactg cttcccggac
     3001 tgagggccgc gcggtggacg gcctctggcc gtggatcgtg atggcggggc tgggggccta
     3061 ccacggcctc aaccccgcca tgggctggct gttcgccgtc tcgctgggca tgcaggaccg
     3121 cgaccgctcg tcggtcacgc gggcgctggt gccgatcgcc atcggccacg agctgtcgat
     3181 cgcgctggtc gcgctgctgg tgatcggcct gggcgtggtc accgacacca ccgccctgca
     3241 cctgggcgcg gcggtcgccc tgatcgcgtt cgggatcttc cgcttcgcca agccgcgggc
     3301 ccacttccgc tgggtcaagg gccgggtcga ccggcgcgag ctcagcgtgt ggtcgttcct
     3361 gatgtccacc gcccacggcg ccggcctgat ggtggccccg gtgctgatcg gctccagcca
     3421 ggcacaggcc tcggaggggc actcgcacgc cgtccaggag atgggcctgg gccggctgtc
     3481 gatccccgag accgtcctgg ccgtgtccgt gcacgtgggg gcgatgctgc tggtcatggg
     3541 cacggtggcg ctgctggtct acgagaagct gggcgtgcgc gtcctgcgca agacgtgggt
     3601 caactccgac cacctgtggg ccgcctcgtt cgtggcggcg ggcgtgatca ccctggtcac
     3661 ctgaggacgt tgaaataccc caggggggta tgctacggtg ggccgtatgg aagcgcacgc
     3721 ccaccaccac gagatgccca cctccggcac cgcgctcacg ggcgtggcca tcagcgcgac
     3781 gctgcactgc ctgaccggct gcgccatcgg cgaggtggcg ggcatggcca tcggcaccgc
     3841 gctcgggttc tcggactggg gcacggtggc gctggccgtg gcgctggcct tcctgttcgg
     3901 ctactcgctg accagcctgc cgctgctgcg cgccggcctg gcgctgagcg tggtggtgcc
     3961 gatcgcgctg gcctcggaca cgctgagcat cgcgaccatg gagatcgtgg acaacgcggt
     4021 catgctggcc atcccgggcg ccatgcacgc aggcctcggc gacgtgctgt tctggggctc
     4081 gctggccttc gccctggccg tcgccggcgt cgtggcggtg ccggtgaacc ggtggctgat
     4141 cgcccgcggg aagggccacg cgaaggttca cgagaccggc atccacggcg gaccgtcgcc
     4201 gcggctcgtg ggcatcatcg ccgcggctgc cttcgcgttc ggctccaccg tcctggtggc
     4261 cgaggcgctg tccgaggacg aacccgcgca cggcggcggg cacgcggccc agggccggct
     4321 ctccgagcgg gccttcctga ccgagatgac cgcgcaccac cagtcggcca tccgcaccgg
     4381 ccaccaggcg caagagcgcg gccagagcgc gttcgtccgc ggcctgggcg ccgacatcgt
     4441 gcgtacgcag gccgccgagg tgcggcagat gcgcgccctc taccggcgcc tggtcggcgg
     4501 tgcgatgccg gcggcgcaga tgcacgacgg tgtccaggcc cgtctcgcgc gctcacgcga
     4561 attcgaccgc gcgttcgtgg accacatgat cccccaccac gtggacgcgg tgcggatggc
     4621 gcgggaggtc aggaccggcg accgcgagct acgcgcgctg gccgcggcga tcgtgaccgc
     4681 gcagcggcgc gagatcggac agatgcgcca cttccgcgac cgcacccacg gcacgcgcga
     4741 ggcgccctcg cccgcggacg acggccacgg cggtcacacc gactgagctt cgggctccgg
     4801 cggcggcgcg cgccgcacca gcaggtacga cagccacagc ccgagcgcga acagcggcag
     4861 ccccatcgcg gtacgcgcga tgcccagggc caccaccgct ccggccaggt agagcggcag
     4921 ctgcacggcc agccggatca cgaacagccc ggaccacatc cagctggcct ggttgtacac
     4981 gcgcatacgc gcgcggtcct cgcgccaggc ggtcttggcc gtgccgtcca gctgcgtggc
     5041 caggtagccg acgatggggt accgggccag gaccgagatg gcgaacgccg cggcgtagcc
     5101 ggcgttgaag atcagccccg ggaggaagaa gttctcggcc ttgccgctgc gcgtggccac
     5161 gaacgcggcc agcgccacgc cgaacaggcc cgacagcgcg tggcggggcg tctcgcgccg
     5221 ggccagccgg gcggcggcca gcgcggcggc caggcccagg gccacgtacg cggacaggtt
     5281 cgtgtcctgc ccggtcacgg tgtaggccac cacgaaggcc aggcccggga gcgcggtctc
     5341 ggccatgccc agggggccgc cggccgcctc gatcaggttc ggtggcggcg gctcctcggt
     5401 ggtgtctcgc gggtcggcca ggcggcccat catggcgagt accgtcaaac catggagatc
     5461 gacctgagtg gacgcacggc cttcgtcagc ggatcgaccc aggggatcgg gcgggcgatc
     5521 gccgagaagc tggccggggc cggggcggac gtcacggtca tcggccgcgg ccgcgagcgc
     5581 gtggacgccg tcgccgcgga gctgggcacg cgcgggatcg cggccgacgt gtcgaccgcc
     5641 gagggcgcgg ccctggtggc cgagcagctc ccggccgtgg acatcctggt gaacaacctg
     5701 ggcatcttcg agccgatccc gatgctggag gtcgacgacg acacctggca gcgctactgg
     5761 gacgtgaacg tgatgtcggc catccggctg acgcgcctgt acgcaccggg catggccgac
     5821 cggggctggg gccgggtcca gttcatggcc agcgactcgg ccgtggtgat cccggccgag
     5881 atggcccact acggggtgac caagaccgcc ctgctggggg tctcgcgcgg cttcgccaag
     5941 gcgctctcgg gcaccggcgt gaccgtgaac acggtgatgg ccggccccac ccacaccgag
     6001 ggcgtcgagc agttcgtgcg cgagctggtc ggcgacgaca aggagtgggg cgaggcccag
     6061 gccgagttca tggccgagca ccggccccac tcgctgatcg agcggctgat cgagcccgag
     6121 gagatcgcca acatggtcgc ctacctgagc tcggacgccg cctcggccac caccggcggg
     6181 gcgctgcgcg tcgacggcgg ctacgtcgac tacatcgtgc cctagtgacg aagatcacat
     6241 aacgtaggag cgtcctgcga tacgacccac gcagggacga tcgaggagag gaatcgtgat
     6301 gcgcgcagac cttccgccgg ggcccagcta tccgtcattg gtccacgcca tcggcttctg
     6361 gacgcggccg ctggcctggc tcgagcgcaa ccgggcccgc tacggcaagc gcttcaccac
     6421 ccgcttcccc ctgtccccgc acttcgtgat cctgtccgag ccggacgagc tcaagcaggt
     6481 cttcaccgcc ccgccggacg tgctgcatcc gggccagggc gcgcgcatcc tggagccgat
     6541 cgtaggcaag aactcggtgc tgctgctgga cgaggacgcc cacatgagcc agcgcaagct
     6601 gatgctcccc gccttccacg gaaagaagat ggcggcgctg gacggcctga tgaccgaggt
     6661 ggccgagcgc gaggtggcct cgtggcccgg gggcaagccg atcgcgctgc acccgcggat
     6721 gcagcggctg acgctggaga tcatcctgcg ggccgccttc ggcct
//
LOCUS       DASUPW010000121         2469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1828380, whole genome shotgun sequence.
ACCESSION   DASUPW010000121 DASUPW010000000
VERSION     DASUPW010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1828380"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VFY44_02765"
     CDS             complement(<1..284)
                     /locus_tag="VFY44_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909387.1"
                     /translation="MQGFLGEERGSTRLALVIGAVFISLLAAAAVPFVFGQAGKAQDT
                     KAQSTVARLAASVQACRVDATGYGDCDDRRELRDLSAVRWGSHPGEAGVLS"
     gene            358..1245
                     /locus_tag="VFY44_02770"
     CDS             358..1245
                     /locus_tag="VFY44_02770"
                     /inference="COORDINATES: protein motif:HMM:NF019454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909388.1"
                     /translation="MTGAPLLMAIPNVSQGRDPARVDGLRTAFGGAAVLDTHTDADHN
                     RSVFTLAAEQGTLADALVAGAAAAVEAIDLNAHAGLHPCVGALDVMPVVYLDGERRGA
                     ACAEALTAAARVGDELELPVFLYGDLATRPEHRERADLRRGGWRRLAERMEGGELVPD
                     FGPPRAHPTAGAVLATARPPLVAFNVDLETDDLELARSVAAEIRESAPGGLTGVRAIG
                     LALAERGRAQVSTNVHDHRATPLAEVVEAVRARAPVAEAELVGLAPQAAFEGFPADVP
                     LRDFDPARHLIENALRSLS"
     gene            1245..1607
                     /locus_tag="VFY44_02775"
     CDS             1245..1607
                     /locus_tag="VFY44_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909389.1"
                     /translation="MAQTKKKRSRKHRGTPAGTIDRAGRPGSGGKAKANAKAKADPKA
                     AARQKRMERINRPPTWQGAVNRAAIAAAVFGVLVVAAFGRPVVQGALLAAFMFVLYIP
                     LGYLTDKAVFNFRNRKKR"
     gene            1610..2248
                     /locus_tag="VFY44_02780"
     CDS             1610..2248
                     /locus_tag="VFY44_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5909390.1"
                     /translation="MEVRMFTVGPVQENTFLINREGSDRALIVDPGEEAPRLLGAIEE
                     AGLTLDGILLTHTHFDHIGAVAPIARATGAEVWVPELEKQVLADINAYVPWPGFGPFE
                     DWEAEHTLAGGEKLELAGFDIDVVFTPGHSHGHVTFSIPDEQAVFSGDVLFQGSVGRV
                     DLPGGDWPTLLESIRTLVDTLPAETAVHPGHMGQTTLAAERATNPFLAELAR"
     gene            2251..>2469
                     /locus_tag="VFY44_02785"
     CDS             2251..>2469
                     /locus_tag="VFY44_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX5909391.1"
                     /translation="MAGGKLQAPRGTFDVLPEDGERRAALRESSDELLGRAGYAPFET
                     PVFEDTEVFARGVGQSTDIVQKEMFTFED"
BASE COUNT          346 a          894 c          916 g          313 t
ORIGIN      
        1 gacagcacgc cggcctcgcc ggggtggctt ccccagcgca cggcgctgag gtcacgcagc
       61 tcgcggcggt cgtcgcagtc gccgtagccg gtcgcgtcca cgcgacaggc ctgcaccgac
      121 gcggccagcc tggcgacggt cgactgggcc ttggtgtcct gcgccttgcc cgcctggccg
      181 aacacgaacg gcacggccgc cgcggccagc agcgagatga acaccgcacc gatgaccagc
      241 gccaggcgcg tcgaaccgcg ctcctctccg agaaacccct gcactttcga gggcatcgac
      301 aggcggcaag gccccttgag cggaccctgg acggattgcc tagggcatga tgcggacatg
      361 accggagcgc cgctgttgat ggccattccc aacgtcagtc agggtcgtga cccggcccgc
      421 gtggacgggc tgcggaccgc gttcggcggg gcggccgtgc tggacacgca caccgacgcc
      481 gaccacaacc gctcggtgtt caccctggcc gccgagcagg gcaccctggc cgacgccctg
      541 gtggccggcg ccgcggcggc ggtcgaggcg atcgacctga acgcgcacgc cggcctgcac
      601 ccgtgcgtgg gggccctgga cgtgatgccc gtggtgtacc tggacggcga gcgccgcggc
      661 gccgcctgcg cggaggcgct gaccgcggcc gcgcgggtgg gggacgagct cgagctgccc
      721 gtgttcctct acggcgacct ggccacgcgg cccgagcacc gcgagcgcgc ggacctgcgc
      781 cggggcgggt ggcgccggtt ggccgagcgt atggagggcg gggagctggt gcccgacttc
      841 gggcctcccc gggcgcaccc cacggcgggg gcggtgctgg ccacggcccg cccgccgctg
      901 gtggcgttca acgtggacct cgagaccgac gacctggagc tggcgcgctc ggtcgcggcc
      961 gagatccgcg agtcggcgcc cgggggcctg accggggtgc gcgccatcgg gctggcgctt
     1021 gccgagcgcg ggcgggccca ggtgtccacc aacgtccacg accaccgcgc gacgccgctg
     1081 gccgaggtgg tcgaggcggt ccgggcacgg gcgccggtgg ccgaggccga gctggtgggc
     1141 ctggccccgc aggcggcctt cgagggcttc cccgccgacg tgccgctcag ggacttcgat
     1201 cccgctcggc acctgatcga gaacgcgtta cgctcacttt cctgatggcc cagaccaaga
     1261 agaagcgctc ccgcaagcac cgtggcaccc ccgccggcac catcgaccgc gccggccgcc
     1321 cgggcagcgg tggcaaggcc aaggccaacg cgaaggccaa ggccgatccc aaggcggccg
     1381 ctcgccagaa gcgcatggag cgcatcaacc gcccacccac gtggcagggc gccgtcaacc
     1441 gggccgccat cgccgccgcc gtgttcggcg tgctggtggt cgcggcgttc ggtcgcccgg
     1501 tggtccaggg cgcgctgctg gccgcgttca tgttcgtgct ctacatcccg ctcggctacc
     1561 tgaccgacaa ggccgtcttc aacttccgca accgcaagaa gcgatgagca tggaagtgcg
     1621 gatgttcacg gtcgggccgg tgcaggagaa caccttcctg atcaaccgcg agggctcgga
     1681 ccgggcgctg atcgtggacc cgggggagga ggcaccgcgg ctgctgggcg cgatcgagga
     1741 ggccgggctg acgctggacg gcatcctgct cacgcacacg cacttcgacc acatcggcgc
     1801 ggtcgccccg atcgcccgcg ccaccggcgc cgaggtgtgg gtgcccgagc tggagaagca
     1861 ggtcctggcc gacatcaacg cctacgtgcc gtggccgggc ttcggcccgt tcgaggactg
     1921 ggaggccgag cacacgctgg ccgggggcga gaagctggag ctggccgggt tcgacatcga
     1981 cgtggtgttc acgccgggtc acagccacgg tcacgtcacg ttctcgatcc ccgacgagca
     2041 ggccgtgttc tcgggcgacg tgctgttcca gggctcggtc ggccgcgtcg acctgccggg
     2101 gggcgactgg ccgacgctgc tggaatccat ccgcacgctg gtcgacaccc tgcccgccga
     2161 gaccgcggtg cacccgggcc acatgggcca gacgacactg gccgccgagc gcgccacgaa
     2221 ccccttcctg gccgagctgg cccgctagcc gtggccggag ggaagctgca ggccccccgc
     2281 gggaccttcg acgtcctgcc cgaggacggc gagcgccgcg ccgcgctgcg cgagagcagc
     2341 gacgagctgc tgggccgcgc gggctacgcg ccgttcgaga cgcccgtgtt cgaggacacg
     2401 gaggtgttcg cgcgcggcgt cgggcagtcc acggacatcg tgcagaagga gatgttcacc
     2461 ttcgaggac
//
LOCUS       DASUPW010000122         4501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_997104, whole genome shotgun sequence.
ACCESSION   DASUPW010000122 DASUPW010000000
VERSION     DASUPW010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997104"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VFY44_02790"
     CDS             complement(<1..295)
                     /locus_tag="VFY44_02790"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5909392.1"
                     /translation="MNPPPNTQTTDLALAARMRLAITRTARRLRQEADAHLTPSQVAA
                     LATIEKRGPLTPSELADIERIQRPTATRVAGHLTERGLVERAGDPGDGRVSLLS"
     gene            complement(429..2006)
                     /locus_tag="VFY44_02795"
     CDS             complement(429..2006)
                     /locus_tag="VFY44_02795"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX5909393.1"
                     /translation="MNHGKMTHREVLEAMSGLLLGLLVAILSSTVVSAALPTIIADLH
                     GSQSSFTWVVTATLLAMTVSTPIWGKLADLFDRKLLVQSALSLYVFGSILAGFAQSTG
                     WLIGCRVIQGLGVGGLMALVQVVLSDLVSPRERGRYMGYLGSVMAVGTVGGPLLGGVI
                     TDSALGWRWCFFVGVPIAAAALVVLQRTLHLPERPQRKVRIDYLGAALISAGVSAVLI
                     WVSLAGHQFDWLSWQSAALVGAGVALLVAAVRVELTRDEPLIPTHLFRERVPVLAAIA
                     SASVGVALFGVAVFLSQYLQISRGESPTASGLLTIPMMVGILISSTLVGRRISKTGRY
                     KKFMVTGATLLTVGLALMGMLDEHTSLIELGAFMSLVGLGVGMVMQNLVLVVQNSVPL
                     EDIGSASALITFFRSLGGATGVSVLGAILGSRASAQIADGLAAKGIHAGAGGAAGSIP
                     DVGSLPGPVQAVVQHAYASGTAEIFLVAAPLSLIALIAIALLPEVPLGTRSGTERLAE
                     ERAAEAEAPAPAAPVPA"
     gene            complement(2003..2470)
                     /locus_tag="VFY44_02800"
     CDS             complement(2003..2470)
                     /locus_tag="VFY44_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5909394.1"
                     /translation="MTNDDTLENLVREVYELAAVRRDMWRRAGFEQAQTIAALNAVRR
                     LGPVRVSAVADDLSVDISVASRQLSALEERGLVQRTTDPGDGRSRLLAVTDAGLESLG
                     QAHQRMVGVFASTVADWDDDDIAGLAAGLHRLREDYARAVAEARVAQPEGAGR"
     gene            2551..3201
                     /locus_tag="VFY44_02805"
     CDS             2551..3201
                     /locus_tag="VFY44_02805"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEX5909395.1"
                     /translation="MNVEIWSDIACPWCYVGKHRFQRALEGFEHRDQVDVTWRAFELD
                     PSAPPERPGTHRDLLASKYGRTADEAQAMLDQMTAMGAAEGLDLRFDRVRSGNTFDAH
                     RVVHLAAFHGAQDAMQERLMRAYLTEGELMSDHDALTRLAAEVGLPAPEVEALLAGDR
                     FAAEVRAEQELAHGFGITAVPFFVVERTLGGAGAQPAEAFAELLAQSWERTQAQPA"
     gene            complement(3198..>4501)
                     /locus_tag="VFY44_02810"
     CDS             complement(3198..>4501)
                     /locus_tag="VFY44_02810"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEX5909396.1"
                     /translation="GGLATFLTMSYILFVNPAILSAAGMPFGAVAVATALVSCVATAT
                     MGLLTNLPFALAPGLGINAVIAFEVIIGRDLPWQVGMSIIVIEGLLALILVVAGFREA
                     VMDAVPTSLKLAIGVGIGLFITLVGLREGGIVVNDPATGIALGDLTNGPALISLAGIL
                     FAAVLTVRGVRGALPLGVVMATVLGLVFGVLEGPDGFIDAPGSGDFSTIGEGLDPSNL
                     EAALTVALIPVIFALFMTDFFDTIGTSVSLGRAGGLMDSEGKLPGTRRMLMVDSGAAA
                     LGGAAGTSSVTTYVEIGAGVAEGARTGLSSLFVAALFLLSVLFIPVIALVGQGVEVSK
                     DLTIHPAVAPALVMVGYLMIRMVGEIDWAAPEDALPAFLIIAGIPLTFSIAAGIGLGM
                     IGYVVVLAATGRVRRVHPLSWVIALLFVGFFAADWLSANVF"
BASE COUNT          707 a         1626 c         1628 g          540 t
ORIGIN      
        1 ccgacaggag gctcacgcgg ccgtcacccg gatcgccggc gcgctcgacc aggccgcgct
       61 ctgtcaggtg gccggccacc cgcgtcgccg tcgggcgctg gatgcgctcg atgtccgcca
      121 gctcgctggg cgtcagcggc ccgcgcttct cgatcgtcgc cagcgccgcc acctgcgacg
      181 gcgtcaggtg cgcatcggcc tcctggcgca gccgccgagc ggtgcgcgtg atggccaggc
      241 gcatccgggc cgcgagggcc aggtcggtcg tctgcgtgtt gggcggaggg ttcattagct
      301 ctggtaatta ccttagcgaa ctagttgcgg atcgtcggcc gcgtcacgac ttccgtcgac
      361 ccggcagacg aaagtcgtga cgctccccgg gggcgcgcta cgggagcgcg acgacggtca
      421 cggggccgtt acgccgggac cggcgcggcc ggcgccggcg cctcggcctc ggccgcccgc
      481 tcctccgcca gccgctcggt cccgctgcgc gtgcccagcg gcacctcggg caacagggcg
      541 atggcgatca gcgcgatcag cgacagcggc gcggccacga ggaagatctc ggcggtcccg
      601 ctggcgtagg cgtgctggac cacggcctgg accgggccgg gcagcgagcc gacgtcgggg
      661 atcgagcccg cggcgccgcc ggcgcccgcg tggatgccct tggccgccag gccgtcggcg
      721 atctgggcgc tggcccgcga gcccaggatg gccccgagca ccgagacgcc ggtcgccccg
      781 ccgagcgagc ggaagaaggt gatgagcgcg ctggccgacc cgatgtcctc caggggcacg
      841 gagttctgca cgaccagcac caggttctgc atgaccatgc cgacgcccaa accgaccaga
      901 ctcatgaagg cgcccagctc gatcaggctg gtgtgctcgt cgagcatgcc catcagggcc
      961 aggcccacgg tcagcagggt cgcccccgtg accatgaact tcttgtagcg gccggtcttg
     1021 ctgatccggc ggccgaccag cgtcgacgag atcaggatgc cgaccatcat cgggatggtc
     1081 aggaggccgg acgcggtcgg gctctcgccg cgcgagatct gcaggtactg gctcaggaac
     1141 acggcgacgc cgaacagcgc cacgcccacg gaggcgctgg cgatggcggc caggacgggg
     1201 acgcgctcgc ggaagaggtg ggtggggatc agcggctcgt cgcgcgtgag ctcgacgcgg
     1261 acggcggcca ccagcagcgc cacgccggcg ccgaccagcg cggccgactg ccacgacagc
     1321 cagtcgaact ggtggccggc cagcgacacc cagatcagca cggcggacac gccggccgag
     1381 atcagggccg cgcccaggta gtcgatcctg accttgcgct gcgggcgctc gggcaggtgg
     1441 agcgtgcgct ggagcaccac cagcgcggcg gcggcgatgg gcacgccgac gaagaagcac
     1501 cagcgccagc ccagggcgga gtcggtgatc acgccgccca gcagcgggcc gccgaccgtg
     1561 ccgaccgcca tgaccgagcc caggtagccc atgtagcgcc cgcgctcacg cggcgagacc
     1621 aggtcggaca gcaccacctg gaccagcgcc atcaggccgc cgacgcccag cccctggatc
     1681 acgcggcagc cgatcagcca gccggtggac tgggcgaagc cggccaggat cgagccgaag
     1741 acgtacagcg acagcgcgct ctggaccagc agcttgcggt cgaacaggtc ggccagcttg
     1801 ccccagatgg gcgtgctgac cgtcatggcc agcagggtcg cggtcacgac ccaggtgaac
     1861 gagctctggc tgccgtgcag gtcggcgatg atcgtgggca gcgccgccga gacgacggtg
     1921 ctggacagga tcgccaccag cagcccgagc agcagcccgg acatggcctc gaggacctcg
     1981 cggtgggtca tcttcccgtg gttcatctgc cggctccttc cggctgggcc acgcgggcct
     2041 cggccaccgc gcgcgcgtag tcctcgcgca ggcggtgcag gccggccgcg aggccggcga
     2101 tgtcgtcgtc gtcccagtcc gccacggtgc tggcgaacac gccgaccatg cgctggtgcg
     2161 cctggcccag ggactccagc ccggcgtcgg tcacggccag cagccgcgag cggccgtcgc
     2221 cgggatcggt ggtgcgctgg accaggccgc gctcctcgag cgcggacagc tggcggctgg
     2281 ccaccgagat gtccaccgac aggtcgtcgg ccaccgcgct gacgcgcacc ggccccaggc
     2341 gccggaccgc gttgagcgcg gcgatggtct gggcctgctc gaagccggcc cggcgccaca
     2401 tgtcgcggcg caccgcggcc agctcgtaga cctcgcggac caggttctcg agggtgtcgt
     2461 cgttcgtcat ctcagtgggc tccagttgct tgctgaatgc aactataaca cttgcgcaat
     2521 gcaagtaccc acgcgtctac gctcaacgac gtgaacgtcg agatctggtc cgacatcgcc
     2581 tgcccctggt gctacgtggg caagcaccgt ttccagcgcg cgctggaggg cttcgagcac
     2641 cgcgaccagg tggacgtgac ctggcgcgcc ttcgagctgg acccctccgc cccgcccgag
     2701 cggcccggca cccaccgcga cctgctggcc tccaagtacg gccgcaccgc cgacgaggcc
     2761 caggccatgc tggaccagat gacggccatg ggcgcggccg agggcctgga cctgcgcttc
     2821 gaccgcgtgc gctcgggcaa caccttcgac gcccaccggg tggtgcacct ggcggccttc
     2881 cacggcgccc aggacgcgat gcaggagcgc ctgatgcgcg cctacctgac cgagggcgag
     2941 ctgatgagcg accacgacgc cttgacgcgg ctggcggccg aagtcggcct gccggccccg
     3001 gaggtcgagg cgctgctggc cggcgaccgc ttcgcggccg aggtgcgcgc cgagcaggag
     3061 ctggcccacg ggttcggcat cacggccgtg cccttcttcg tggtcgagcg cacgctgggc
     3121 ggcgccggcg cccagccggc cgaggccttc gccgagctgc tggcgcagtc ctgggagcgc
     3181 acccaggccc agccggccta gaacacgttg gcgctgagcc agtcggcggc gaagaagccg
     3241 acgaacagca gcgcgatgac ccagctgagc gggtgcaccc ggcgcacgcg gccggtggcg
     3301 gccaggacca ccacgtagcc gatcatcccc agcccgatgc ccgccgcgat cgagaacgtc
     3361 agcgggatgc cggcgatgat caggaacgcg ggcagcgcgt cctccggggc ggcccagtcg
     3421 atctcgccga ccatgcggat catcaggtag ccgaccatga ccagcgccgg cgccacggcc
     3481 gggtggatgg tcaggtcctt gctgacctcg accccctgcc ccaccagcgc gatcaccggg
     3541 atgaacagga ccgacagcag gaacagggcg gccacgaaca gcgagctcag cccggtgcgc
     3601 gcgccctcgg ccacgcccgc gccgatctcg acgtaggtgg tcacgcttga ggtgcccgcg
     3661 gccccgccca gcgcggcggc gccggagtcg accatgagca tccggcgcgt gcccggcagc
     3721 ttgccctcgg agtccatgag cccgccggcg cggcccagcg acaccgaggt gccgatggtg
     3781 tcgaagaagt cggtcatgaa cagggcgaag atcaccggga tcagcgccac cgtcagcgcg
     3841 gcctcgaggt tggacgggtc caggccctcg ccgatggtgg agaagtcgcc ggagccgggg
     3901 gcgtcgatga agccgtccgg gccctccagc accccgaaga ccagccccaa cacggtggcc
     3961 atgaccacgc cgaggggcag cgccccgcgc acgccgcgga cggtgagcac ggcggcgaac
     4021 aggatgccgg ccagcgagat cagcgccggc ccgttggtca ggtcgccgag cgcgatgccg
     4081 gtggccgggt cgttgaccac gatgccgccc tcgcgcagcc ccacgagcgt gatgaacagg
     4141 ccgatgccga cgccgatggc cagcttgagc gaggtgggca cggcgtccat gacggcctcg
     4201 cggaagcccg cgacgaccag gatcagcgcc agcagtccct cgatgacgat gatcgacatg
     4261 ccgacctgcc agggcaggtc gcggccgatg atgacctcga acgcgatcac cgcgttgatg
     4321 cccagcccgg gagccagggc gaacggcagg ttggtcagca ggcccatggt cgcggtggcc
     4381 acgcacgaga ccagggcggt ggccaccgcc acggctccga agggcatccc ggcggccgac
     4441 aggatcgccg ggttcacgaa caggatgtag ctcatggtca ggaacgtggc caggccgccg
     4501 a
//
LOCUS       DASUPW010000123         2270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_27426, whole genome shotgun sequence.
ACCESSION   DASUPW010000123 DASUPW010000000
VERSION     DASUPW010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27426"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VFY44_02815"
     CDS             <1..675
                     /locus_tag="VFY44_02815"
                     /inference="COORDINATES: protein motif:HMM:NF015637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYG1 family protein"
                     /protein_id="HEX5909397.1"
                     /translation="GIGYASFGLIWREYGERVCGGDAELAAQLDQSLVQGIDANDTGQ
                     QVFQTLIEGVRPLTVNGVIGGLNPRWDEDLTDDQERARFDQAVALAGPIIEREVASAA
                     SGLRAARLVRQGIEAAEDPRLIELADNVPWKSVVVPEAPEALYVIYPKRQGFGLETVP
                     RELGSFANRQDLPEPWAGLDGPELAELTGVDDAVFCHAKRFLAVARTREGIGELARQA
                     LAQSSA"
     gene            complement(752..1177)
                     /locus_tag="VFY44_02820"
     CDS             complement(752..1177)
                     /locus_tag="VFY44_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909398.1"
                     /translation="MRRLRGQTFHPSTAISLLALFVALGGTAFAATGDRGGNGAGATV
                     SEAISDQKLRIRQASIGVDGGTANGNYNSRTVSVKCRRGEVALSVSTQWDSPDNNELN
                     TVFARLSTTRSGTPNGAVARGSSDTPGVATFRVQVLCAG"
     gene            complement(1244..1789)
                     /locus_tag="VFY44_02825"
     CDS             complement(1244..1789)
                     /locus_tag="VFY44_02825"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEX5909399.1"
                     /translation="MNHLSAHLRCSGSHDALPFHRDCPACVRERMRGTPKEGAVVPAP
                     ARAGLTALVLAASAALLGARSTDSAQAQTTETTPSVDEAPQMVSPEDLDDQLADEDKK
                     KPEADDQGREDAQAGALKGGSYVVKRGDSLWGIAKANLGGSPSNARLAGEVKRLWDLN
                     DVAIGTGNPDLIYTGQRLKLR"
     gene            complement(1866..>2270)
                     /locus_tag="VFY44_02830"
     CDS             complement(1866..>2270)
                     /locus_tag="VFY44_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEX5909400.1"
                     /translation="GPVDSAAPAPTPRKRVAPIRHQRETRADPKRARPAPRFRRPVAP
                     VRAPSGAPVQAPPASAPAPVTTPSSKAGSEESHTVQAGETLWSIASARLGPGASAAQI
                     AREVQRIWDLNARAIGTGDPSLIGVGVQLRFD"
BASE COUNT          275 a          793 c          864 g          338 t
ORIGIN      
        1 gggatcggct acgcgtcgtt cggcctgatc tggcgcgagt acggcgagcg cgtgtgcggc
       61 ggcgacgccg agctggcggc gcagctggac cagtcgctgg tgcagggcat cgacgccaac
      121 gacaccggcc agcaggtgtt ccagacgctg atcgagggcg tgcgcccgct gacggtgaac
      181 ggcgtgatcg gcgggctcaa cccgcgctgg gacgaggacc tgaccgatga ccaggagcgc
      241 gcccgcttcg accaggccgt ggcgctggcc gggccgatca tcgagcgcga ggtggcctcg
      301 gcggcgtcgg ggctgcgcgc agcgcggctc gtacgccagg gcatcgaggc cgcggaggac
      361 ccgcggctga tcgagctggc ggacaacgtg ccgtggaagt ccgtcgtggt gcccgaggcc
      421 ccggaggcgc tgtacgtgat ctaccccaag cgccagggct tcggcctgga gacggtgccg
      481 cgcgagctgg gctcgttcgc caaccgccag gacctgcccg agccctgggc ggggctggac
      541 gggccggagc tggcggagct gaccggcgtc gacgacgccg tgttctgcca cgccaagcgg
      601 ttcctggcgg tggcccgcac gcgcgagggc atcggcgagc tggcgcggca ggcgctggcg
      661 cagtcctcgg cctagccggc cgcccgcccg gggagcgccg ttcatgcgtt tggtccgctc
      721 tggcctccca aacgcatgaa ccgttgacgg atcagccggc gcagagcacc tggacgcgga
      781 acgttgcgac gccgggcgtg tccgagctgc cacgggccac cgccccgttc ggcgtgcccg
      841 accgcgtcgt cgacaggcgg gcgaacacgg tgttcagctc gttgttgtcg ggcgagtccc
      901 actgggtgct caccgacagc gcgacctcgc cccgcctgca cttcaccgac acggtacggc
      961 tgttgtagtt cccgttcgcc gtgccgccgt cgacgccgat cgaggcctgg cggatcctca
     1021 gcttctggtc gctgatggcc tcggagaccg tcgcgccggc cccgttgccg ccccggtcac
     1081 cggtggccgc gaacgccgtg ccgcccagcg ccacgaacag cgccagcagc gagatcgccg
     1141 tgctcgggtg gaaggtctgc cctcgaagtc gtcgcatcgc ctgctcccgt cgtcgccatg
     1201 aacgttccgt tccggcaacc ctgccacggg gcgggcgatg cggtcagcgc agcttcaggc
     1261 gctggcccgt gtagatcagg tccgggttgc cggtcccgat cgccacgtcg ttgaggtccc
     1321 acagccgctt gacctcgccg gcgaggcggg cgttgctggg cgagccaccc aggttcgcct
     1381 tggcgatccc ccacagcgag tcgccccgct tgaccacata gctgccgccc ttgagcgcgc
     1441 ccgcctgcgc gtcctcgcgg ccctggtcgt cggcctcagg cttcttcttg tcctcgtcgg
     1501 cgagctggtc gtccaggtcc tcgggcgaga ccatctgcgg agcctcgtcc acggagggcg
     1561 tggtctcggt ggtctgggcc tgcgccgagt cggtggagcg ggcgccgagc agggcggccg
     1621 aggcggccag taccagggcg gtcaggccgg cgcgcgcggg cgccggaacc acggcgcctt
     1681 ccttcggcgt cccgcgcatg cgctcgcgca cgcacgcggg gcagtcacgg tggaacggca
     1741 gggcgtcatg gctgccggag cagcgcaggt gggcggagag gtggttcacg ggaccgggcc
     1801 atcttccctg ttcgggcgaa atgacctatg gggatgtgcg ccacgttggg gccgtggcgc
     1861 gtccgtcagt cgaatcgcag ctggacgccg accccgatca ggctggggtc gccggtgccg
     1921 atcgcgcgag cgttgaggtc ccagatgcgc tggacctcgc gagcgatctg ggcggcgctg
     1981 gcccccggcc cgagccgcgc ggaggcgatc gaccacaacg tctccccggc ctggaccgtg
     2041 tgggattcct cactgccggc tttggacgac ggggtggtta cgggtgccgg ggcgcttgcc
     2101 gggggcgcct gcaccggtgc accggatggc gcccggacgg gggccaccgg tcggcggaac
     2161 cggggcgcag ggcgggcgcg cttcgggtct gcccttgtct cgcgctgatg tctgatcggc
     2221 gcgacccgct tgcgcggtgt cggcgctggc gccgcggagt cgaccggacc
//
LOCUS       DASUPW010000124         7604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_997894, whole genome shotgun sequence.
ACCESSION   DASUPW010000124 DASUPW010000000
VERSION     DASUPW010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7604
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997894"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VFY44_02835"
     CDS             <1..313
                     /locus_tag="VFY44_02835"
                     /inference="COORDINATES: protein motif:HMM:NF007495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HEX5909401.1"
                     /translation="FDKIDIRVGRVVEVEDFPEARKPAWKLRVDFGPEIGVKRTSAQV
                     TNYSREELEGRLVVGVVNFPARQIGPVMSEFLVLGVPDEEGRVILLSPSTEVPLGGRM
                     F"
     gene            complement(594..1508)
                     /locus_tag="VFY44_02840"
     CDS             complement(594..1508)
                     /locus_tag="VFY44_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEX5909402.1"
                     /translation="MERTERADFQATTDPGLMESADRGQSQLLGYGELYSLWERQQWA
                     VQDIDFTQDRIDWHERIPAEERYQRMAGLSSFFIGEQRVAAELGPMMRAAPQEDMRIF
                     LCTQIADEARHVAFFDRFYSEVGVLEADDLGARLEETSVHVNESFERLFDQMLKDRVD
                     RLAVEPEDLETLVEAVTLYHMIIEGMLALTGQHFIIEYNEQQGTLPGFVEGFNNVARD
                     EHRHVAFGAAFLRDMAARDPKYLDAVQRTLVECGPAADGVLTPKWAEEQGVDDDTEIF
                     GYSLADTRAFAMKALERRLKVIGLAPVA"
     gene            complement(1546..2070)
                     /locus_tag="VFY44_02845"
     CDS             complement(1546..2070)
                     /locus_tag="VFY44_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909403.1"
                     /translation="MRRRALAAVAAAAVLAVAGCGGDDEKDEAGADTTATTEQTETTD
                     TATTETQPPATETEPAETGEDSGGTKAPGEDQPGGAGDEIPNATQALVTGRAGKVGPA
                     TVSVPPFIAITVELRSADGRDYALSGGGRSLAAGEGENSDTVSFDGLRPGRKLVLSGP
                     QGRVTISANAEPGP"
     gene            complement(2067..2744)
                     /locus_tag="VFY44_02850"
     CDS             complement(2067..2744)
                     /locus_tag="VFY44_02850"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX5909404.1"
                     /translation="MDIRFLGQSCFEFSAGDTRLLVDPFLTGNPKAAASADEVDPTHI
                     LLTHGHADHLGDTVDIAKRTGATVIAIVEMANEIGGAGVENVVDPNLGGTVEFDGGWV
                     RLVPAWHTAVTPDGTPHTPAGLVIHLDGKTVYHLGDTCLFSDLALPGKRDALDLAIVP
                     IGGHYTMDRHDAVQAVDLVGAPAVIPCHYDTFPPIEADAAAFKADVEDQTKATVTVLE
                     PGDTSAL"
     gene            complement(2789..3115)
                     /locus_tag="VFY44_02855"
     CDS             complement(2789..3115)
                     /locus_tag="VFY44_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909405.1"
                     /translation="MTDRPFTNAELDAAIQRLTEPGRLRDAEEIVMDRAPELQRILAE
                     ALHAAGWFGEAHDANVRTAAAHATEEDRVTALRTLLAEENRIGMLVGVAVGWELAREL
                     ELDRDR"
     gene            complement(3112..3897)
                     /locus_tag="VFY44_02860"
     CDS             complement(3112..3897)
                     /locus_tag="VFY44_02860"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5909406.1"
                     /translation="MSQPDLFVVCKNCGSEVSPYVTECPYCGQRVRKRAPKLERDEPG
                     ADARPKERRRMPKLSRLRPAEIEGIAPDTRPVATFVLIGLSLAISLALAGGLTFAEVG
                     GIVVPLDDEPWRYATSSFVHDSLTYQFATLVAVGVFGTLLERRFGWFAPILVFVAAGA
                     AGMAAAVQLNLGPLGESNALYAALGANGAALGLLTAWLVDDRRAARRGDQRGNDLVGV
                     AVIAALLLVLPLAVTEANPVAGPVGAAVGALLAPVLGLLSRRA"
     gene            complement(3920..5047)
                     /locus_tag="VFY44_02865"
     CDS             complement(3920..5047)
                     /locus_tag="VFY44_02865"
                     /inference="COORDINATES: protein motif:HMM:NF010039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HEX5909407.1"
                     /translation="MEHRFTGPSYTLGIEEELMIVDAETLDLTNSIEEMLKDVQDAGS
                     AGDVKPELMESVCEIATAPCRDAAEAGQQLTELRSTVQEVAEKRGWAIGAAGTHPFAL
                     WEDQRIVSRPRYRDLISGLQFVARQELIFGIHVHVGVDDPDKAIHVVNGMRVHLPVLL
                     GLSANSPFWRAQSTGMLSARTPIFRAFPRVGIPPRYEDFDDWSRRIEFMIESRTINDY
                     TYLWYDVRPHPKFGTVEIRVMDSQTRLEHTLGLAALVQAMVKELAEHFDSGEPLSRYP
                     YEMLDENKWLAARHGLDGELVDLPSQERVPTKALARRLLDRLHGHAQDLGSANEFEAV
                     RDLLDKGNGGSRQKVVYEANHDLREVVGEIVEKTSPHSPAE"
     gene            5129..6220
                     /locus_tag="VFY44_02870"
     CDS             5129..6220
                     /locus_tag="VFY44_02870"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF006707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEX5909408.1"
                     /translation="MAATDTTSSQLEELLGDDASDLLGHTCQTIDKSRLYLPGPDFLD
                     RTFVDSDRSSRTIRNLQWLADSGRLAGTGYVSILPVDQGIEHSGGASFGPNPAYFDPE
                     ALVELAVEGGCNAVASTLGVLGAVSRKYAHKIPFIVKLNHNELLSYPNTFDQVMFGSV
                     QRAYDMGAAGVGATIYFGSEQSRRQLVETSQAFEEAHRLGMFTVLWCYLRNDDFKVDG
                     TDYSTAADMTGQANHMGVTIEADIIKQKLPENNGGFKAVAEATGQSYGKTNAAMYDKL
                     SSENPIDLTRYQLINCYMGRAGLINSGGASSGESDLAEAVRTAVINKRAGGTGLISGR
                     KAFQRPTDEGIGLLNAIQDVYLNRDVTIA"
     gene            6398..6637
                     /locus_tag="VFY44_02875"
     CDS             6398..6637
                     /locus_tag="VFY44_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909409.1"
                     /translation="MRMRQSIAQFEADFREEAADSVVRREQLRRNAVHRSRARRVERV
                     HKAGTARFAALWVAILATTVIVTLVMFQVLARVAG"
     gene            complement(6754..6984)
                     /gene="csrA"
                     /locus_tag="VFY44_02880"
     CDS             complement(6754..6984)
                     /gene="csrA"
                     /locus_tag="VFY44_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004155916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HEX5909410.1"
                     /translation="MLVLTRKANQSIMIGDGIQVSVLAVMGEKVRIGIEAPRSVPVFR
                     KEVWIEIQRDQAEGGEDVSDEVQKALEELKGD"
     gene            7188..>7604
                     /locus_tag="VFY44_02885"
     CDS             7188..>7604
                     /locus_tag="VFY44_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909411.1"
                     /translation="MRQGRTDAWIAHQLEVSAKDIAGFKREHDLAPGEEATSAESDLF
                     GPRIDEDEPDLRAEDEAAVAAALEAEQDEPDEDEDDADTGDGDADDSGADSDDENGEG
                     GSGQRRRRRGRRGGRGRGRAGGGGGGKALEGTFDHGE"
BASE COUNT         1097 a         2717 c         2660 g         1130 t
ORIGIN      
        1 cttcgacaaa atcgacatcc gcgtcggccg cgtggtcgag gtcgaggact tccccgaggc
       61 gcgcaaaccc gcctggaagc tgcgcgtgga cttcgggccc gagatcggcg tcaagcgcac
      121 ctcggcccag gtgaccaact actcccgcga ggagctcgag ggccgcctcg tggtcggcgt
      181 ggtcaacttc ccggcgcgcc agatcgggcc cgtgatgtcc gagttcctgg tcctgggcgt
      241 gccggacgag gagggccgcg tgatcctgct cagcccctcg accgaggtgc cgctgggcgg
      301 gaggatgttc tgaccggtgc ccgtaccggc gggtcgggtg gaaacagcgg ctgggtaggt
      361 cggccgacga aaggcggagg ggctgtgacc gttcgcgcac ttccgtaacc ctccccgggc
      421 cgcgcggtga ctttctctcg ctcagcggga gcaatgcgac gcgcggttgg cggtggggct
      481 gtcaggtcgg accgggtgtg acgggagcgc gatgaacgtg acaggcggcc cccgccctac
      541 gcagccacag tttcgctgat cccgcagggg ccgcctggcg gcagaacgcc gtgctaggcc
      601 accggcgcca gcccgatgac cttcaaccgc cgctccagcg ccttcatcgc gaacgcgcgc
      661 gtgtcggcca gcgagtagcc gaagatctcg gtgtcgtcgt ccacgccctg ctcctcggcc
      721 cacttcggcg tgagcacgcc gtcggcggcc gggccgcact ccaccagcgt gcgctggacg
      781 gcgtccaggt acttgggatc acgcgcggcc atgtcgcgca ggaacgctgc gccgaaggcc
      841 acgtgccggt gctcgtcgcg ggccacgttg ttgaagccct cgacgaagcc gggcagcgtg
      901 ccctgctgct cgttgtactc gatgatgaag tgctggccgg tcagggccag catcccctcg
      961 atgatcatgt ggtagagcgt cacggcctcg accagcgtct ccaggtcctc gggctccacc
     1021 gccagccggt cgacgcggtc cttgagcatc tggtcgaaga gccgctcgaa gctctcgttc
     1081 acgtgcacgc tggtctcctc cagccgcgcg cccaggtcgt ccgcctccag cacgcccacc
     1141 tcggagtaga agcggtcgaa gaacgcgacg tgtcgcgcct cgtccgcgat ctgggtgcac
     1201 aggaagatgc gcatgtcctc ctggggagcg gcgcgcatca tcgggcccag ctccgcggcc
     1261 acgcgctgct cgccgatgaa gaacgaggac agcccggcca tgcgctggta gcgctcctcg
     1321 gccgggatgc gctcgtgcca gtcgatccgg tcctgggtga agtcgatgtc ctggaccgcc
     1381 cactgctggc gctcccacag ggagtacagc tcgccgtagc ccagcagttg cgactggccg
     1441 cggtcggccg actccatcag ccccgggtcg gtcgtggcct ggaagtccgc cctctctgtg
     1501 cgttccatct gccgaccgta accgaaaact ggccggtcgg ccaagttacg gcccgggctc
     1561 ggcgttggcc gagatcgtga cccgtccctg gggccccgag agcaccagct tgcggcccgg
     1621 gcgcaggccg tcgaaggaca cggtgtcgga gttctcgccc tcccccgcgg ccagcgacct
     1681 gccgccgccg ctgagcgcgt agtcgcggcc gtcggccgag cgcagctcca ccgtgatggc
     1741 gatgaacggc ggcacgctga cggtggccgg gccgaccttg ccggcgcgcc cggtgaccag
     1801 ggcctgggtc gcgttgggga tctcgtcgcc cgcgccgccg ggctggtcct cgcccggcgc
     1861 cttggtgccg ccgctgtcct caccggtctc ggccggctcg gtctcggtgg ccggcggctg
     1921 cgtctccgtg gtcgcggtgt cggtggtctc ggtctgctcg gtggtcgccg tggtgtccgc
     1981 gcccgcctcg tccttctcgt cgtcgccccc acagcccgcg acggccagga ccgcggccgc
     2041 ggccacggcc gccagagcgc gacgcctcac agcgcgctgg tgtcgccggg ctcgagcacg
     2101 gtgacggtgg ccttcgtctg gtcctcgacg tcggccttga acgccgcggc gtccgcctcg
     2161 atgggcggga aggtgtcgta gtggcagggg atcaccgcgg gcgcgccgac caggtccacg
     2221 gcctgcacgg cgtcgtggcg gtccatcgtg tagtgcccgc cgatcggcac gatcgccagg
     2281 tcgagcgcgt cgcgcttgcc cggcagcgcc aggtcggaga acaggcaggt gtcgcccagg
     2341 tggtagaccg tcttgccgtc cagatggatc accaggccgg cgggggtgtg cggggtgccg
     2401 tcgggggtca ccgcggtgtg ccaggccggg accaggcgca cccagccgcc gtcgaactcc
     2461 accgtgccgc ccaggttcgg gtcgaccacg ttctccacgc cggcgccgcc gatctcgttg
     2521 gccatctcca cgatcgcgat caccgtggcg cccgtgcgct tggcgatgtc gaccgtgtcg
     2581 cccaggtggt cggcgtggcc gtgggtcagc aggatgtggg tcgggtccac ctcgtcggcc
     2641 gaggcggcgg ccttggggtt accggtcagg aaggggtcga ccagcaggcg ggtgtcgccg
     2701 gcgctgaact cgaagcagga ctggcctagg aagcggatgt ccacgtgttt ctcctctgct
     2761 cgggactgac gcgaaagctg gggcgagcct agcggtcgcg atccagctcc aactcgcgcg
     2821 ccagctccca gccgacggcg accccgacca gcatcccgat gcggttctcc tcggccagca
     2881 gcgtgcggag cgcggtgacc cggtcctcct cggtcgcgtg cgcggcggcg gtgcgcacgt
     2941 tggcgtcgtg ggcctcgccg aaccagcccg cggcgtgcag cgcctcggcc aggatccgct
     3001 gcagctcggg cgcgcggtcc atgacgatct cctcggcgtc gcggaggcgg ccgggctcgg
     3061 tcaggcgctg gatcgcggcg tccagctccg cattggtgaa cggccggtcg gtcatgcgcg
     3121 tcgggacagg agccccagca ccggcgccag cagggcgccg accgccgccc ccacgggccc
     3181 cgccaccggg ttggcctcgg tgaccgccag cggcagcacc agcaacagcg cggcgatcac
     3241 ggccacgccg accaggtcgt tgccgcgctg gtccccgcgc cgcgcggcgc ggcggtcgtc
     3301 caccagccac gcggtcagca ggcccagggc cgcgccgttg gcgcccagcg cggcgtagag
     3361 cgcgttggac tcgccgagcg ggcccaggtt cagctgcacc gcggcggcca tgccggcggc
     3421 cccggcggcc acgaacacca ggatcggggc gaaccagccg aagcggcgct cgagcagggt
     3481 gccgaacacc cccaccgcca cgagggtggc gaactggtag gtcaggctgt cgtggacgaa
     3541 cgaggaggtg gcatagcgcc acggctcgtc gtccagcggc accacgatgc cgccgacctc
     3601 ggcgaaggtc aggccgccgg ccagcgccag cgagatggcc agcgacaggc cgatgagcac
     3661 gaacgtggcc accggccggg tgtcgggcgc gatcccctcg atctcggccg gccgcagccg
     3721 cgagagcttg ggcatcctgc gccgctcctt gggccgcgcg tccgcgcccg gctcgtcgcg
     3781 ctcgagcttg ggcgcccgct tgcgcacgcg ctggccgcag tacgggcatt cggtcacgta
     3841 cgggctgacc tcggacccgc agttcttgca cacgacgaag aggtcgggct gactcaccag
     3901 atggagggta cgcgacgggc tattcggcgg gcgaatgcgg agaggtcttc tccacgatct
     3961 cgcccacgac ctcgcgcagg tcgtggttgg cctcgtagac caccttctgg cgcgagccgc
     4021 cgttcccctt gtccagcagg tcccggaccg cctcgaactc gttggccgag cccaggtcct
     4081 gggcgtggcc gtggaggcgg tccagcagcc gccgcgccag cgccttggtg ggcacccgct
     4141 cctggctggg caggtccacc agctcgccgt cgaggccgtg gcgcgcggcc agccacttgt
     4201 tctcgtcgag catctcgtac gggtagcgcg agagcggctc gcccgagtcg aagtgctcgg
     4261 ccagctcctt gaccatggcc tgcaccagcg cggccaggcc cagcgtgtgc tccaggcggg
     4321 tctgcgagtc catgacgcgg atctcgaccg tcccgaactt ggggtgcggg cgcacgtcgt
     4381 accagaggta ggtgtagtcg ttgatcgtgc gcgactcgat catgaactcg atccggcgcg
     4441 accagtcgtc gaagtcctcg tagcgcggcg ggatgcccac gcgcgggaag gcgcggaaga
     4501 tcggggtgcg ggcgctgagc atcccggtcg actgggcgcg ccagaacggc gagttggccg
     4561 acagcccgag cagcaccggc aggtgcaccc gcatcccgtt gaccacgtgg atggccttgt
     4621 cggggtcgtc cacgccgacg tgcacgtgga tgccgaagat cagctcctgc cgggccacga
     4681 actgcaggcc ggagatcagg tcgcggtagc gcggccgcga gacgatccgc tggtcctccc
     4741 acagcgcgaa cgggtgcgtg cccgcggcgc cgatggccca gccgcgcttc tcggccacct
     4801 cctggacggt gctgcgcagc tcggtcagct gctggccggc ctcggcggca tcccggcacg
     4861 gggcggtcgc gatctcgcac accgactcca tcagctcggg cttgacgtcc ccggccgacc
     4921 cggcgtcctg gacgtccttg agcatctcct cgatcgagtt ggtcagatcg agggtctccg
     4981 catccacgat catgagctct tcctcgatcc ccagggtgta cgaggggccc gtgaagcggt
     5041 gctccatgcg cgggagccta cccagcccgg cgggcggccc ctacgatgca acgaagtacg
     5101 caagcgacgt ctcgaagagg agatccccat ggccgcaacc gacacgacct catcgcagct
     5161 ggaagagctc ctgggagacg acgccagcga cctgctgggt cacacctgtc agaccatcga
     5221 caagtcccgg ctctacctgc cgggccccga cttcctggac cgcacgttcg tggacagcga
     5281 ccgctcctcg cggaccatcc gcaacctgca gtggctggcc gacagcggcc gcctggccgg
     5341 caccggctac gtcagcatcc tcccggtcga ccagggcatc gagcactcgg gcggcgccag
     5401 cttcggcccc aacccggcct acttcgaccc ggaggccctg gtcgagctgg cggtcgaggg
     5461 cggctgcaac gccgtggcct ccaccctcgg cgtcctgggt gcggtcagcc gcaagtacgc
     5521 ccacaagatc cccttcatcg tcaagctcaa ccacaacgag ctgctgagct accccaacac
     5581 cttcgaccag gtcatgttcg ggagcgtcca gcgcgcgtac gacatgggcg cggccggcgt
     5641 gggcgcgacc atctacttcg gcagcgagca gagccggcgc cagctcgtgg agacctcgca
     5701 ggccttcgag gaggcccacc gcctgggcat gttcaccgtg ctctggtgct acctgcgcaa
     5761 cgacgacttc aaggtcgacg gcacggacta ctccaccgcc gccgacatga cgggccaggc
     5821 caaccacatg ggcgtgacca tcgaggccga catcatcaag cagaagctgc ccgagaacaa
     5881 cggcggcttc aaggcggtcg ccgaggccac gggccagagc tacggcaaga ccaacgccgc
     5941 gatgtacgac aagctcagca gcgagaaccc gatcgacctg acccgctacc agctgatcaa
     6001 ctgctacatg ggccgcgccg ggctgatcaa cagcggtggg gcctcgtctg gcgagagcga
     6061 cctggccgag gccgtgcgca ccgccgtgat caacaagcgc gccggcggaa cgggcctgat
     6121 ctcgggccgc aaggccttcc agcgccccac cgacgagggc atcggcctgc tgaacgcgat
     6181 ccaggacgtg tacctcaatc gggacgtcac gatcgcctag gcgtcgagac gacccttggc
     6241 cgcccttcgg ggccccggcc aaccggccat cgggcggcga gccgccgtct acggattggg
     6301 ctggaagccc gatccgtccg caggctctcc tcggtcgatc tgtgccaatt ggcacagatt
     6361 gagcgcaggc ttgcagtggt agcctctgca accgatcatg cggatgcggc agtcgatagc
     6421 gcagttcgag gcggatttcc gggaggaagc cgccgattcc gtcgtgcgcc gggagcagtt
     6481 gcgccgcaac gcggtgcacc ggtcgcgggc ccgccgcgtc gagcgcgtgc acaaggcggg
     6541 caccgcccgc ttcgccgcgc tgtgggtcgc catcctggcc accacggtca tcgtgaccct
     6601 ggtgatgttc caggtcctcg cccgcgtcgc gggctagccg cctgcgaacc gtcgcgctgc
     6661 tgcgtcctcc gtcgcccgtg tgccgagcac tacgggctcc ctgcgtcctt gcatccctcg
     6721 gttctcgccg gctcgatggc cgaccgggat gcgtcagtcg cccttcagtt cctccagggc
     6781 cttctggacc tcgtccgaga cgtcctcgcc cccttcggcc tggtcgcgct ggatctcgat
     6841 ccagacctcc ttgcggaaga ccgggaccga tcgcggggcc tcgatgccga tgcgaacctt
     6901 ctcgcccatc accgcaagga cggacacctg gatgccgtct ccgatcatga tgctctggtt
     6961 tgccttgcgc gtgagaacga gcactcccgg ggcctccctc cctgggtctg gcggcatccg
     7021 tgccgcttga acgtgagggg tcagtatgcc ccaggttggc tgcccgcaca cgctttccta
     7081 cgaagtgccc ggtgaggggc ctcttggagt tgcctggtcg ttggtagtct gcgcgcctct
     7141 tggcacgcag atcactcaaa ccacagctca accagatccg gacctgggtg cgtcaaggac
     7201 gcaccgacgc gtggatcgcc caccagctgg aagtctcggc gaaggacatc gccggcttca
     7261 agcgcgaaca cgacctggct cccggcgagg aagccacctc ggcggagtcc gatctgttcg
     7321 gcccccgcat cgacgaggac gagcccgatc tccgggccga ggacgaggcc gccgtggccg
     7381 ccgcgctcga ggccgagcag gatgagcccg acgaggacga ggacgacgcg gacaccggtg
     7441 acggcgacgc ggacgactcc ggggccgatt cggacgacga gaacggcgag ggcggctcgg
     7501 gtcagcgcag gcgcaggcgc gggcgccgcg gcggacgcgg gcgcggacgt gccggcggcg
     7561 gcggtggcgg caaggcgctc gagggcacct tcgaccacgg cgag
//
LOCUS       DASUPW010000125         3506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1274755, whole genome shotgun sequence.
ACCESSION   DASUPW010000125 DASUPW010000000
VERSION     DASUPW010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274755"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VFY44_02890"
     CDS             <1..1182
                     /locus_tag="VFY44_02890"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEX5909412.1"
                     /translation="FGRRPISRAWLAVVFPALTLNYLGQGALLVNEPSAVSNPFFLLV
                     PDWALIPMVALATLATVIASQAVISGAYSVSHQAARLGFLPRLRVLHTSAREEGQIYV
                     PLVNWLLMVAVLALVLTFQSSEKLAAAYGVAVIGTITVTTTLFFTLQWVRDRRPHWQV
                     AAVGALFLAFVLVFLAANLVKIGEGGYLPVGIGLVIFTVLSTWRRGRKLADANRDEVE
                     GSLQEFVNDLHNHEIPVRRVPGCAVFLSRGEGSTPLALRANVEHNHTLHRHTVILTLL
                     SLRTPRIGAEDRVTVDDLGFSDDGITHVTARFGYLETMSLPSVLRQASEKGLEGGKVD
                     VEKASYFVSAPELRITDAPGMAQWRKHLFLATTRLTSDPVEFFDLPRGNTVVMGAEID
                     L"
     gene            complement(1393..1992)
                     /locus_tag="VFY44_02895"
     CDS             complement(1393..1992)
                     /locus_tag="VFY44_02895"
                     /inference="COORDINATES: protein motif:HMM:NF014669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEX5909413.1"
                     /translation="MSTASSAALPRRSVLADAIPGALARDIALVIGGAAFMALMAQVT
                     IKMPGELVPITGQTLGVGLIGATLGARRGVSAMSLYAVLGLFLPVYADGGSGWEVIWG
                     ASGGYIIGFILATYVIGLMAERGADRKVLTAFAAFVAAQLIIFGIGVPWLKVSADLTW
                     GDAIHSGFTIFILGGLVKAAIGAAVLPSAWHFVRKADER"
     gene            2012..3286
                     /gene="pepT"
                     /locus_tag="VFY44_02900"
     CDS             2012..3286
                     /gene="pepT"
                     /locus_tag="VFY44_02900"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: protein motif:HMM:NF009920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEX5909414.1"
                     /translation="MPDSAPFTSPLAQQLSEEVTERLVRYARVDTQSSREGTGTPSTE
                     CQWDLARLLEGELAELGLEDIELDEHGYVFATLPATVEGTPTIGLLAHIDVSPDAPAE
                     GVEPIVHRGYDGGVIELPRGGTRLDPATVPGLAEAKGQDLVTSSGDTLLGADDKAGMA
                     EVMTAVAHLAANPGLPRPAIRVGFTPDEEIGKGPILFDVERFGARCAYTLDGSDLGEM
                     QAESFTGSEVVVRIKGVDVHPGSAKDILINATRLAGEVLAALPADLTPERTEGRQGFL
                     HPVEIAGDAVEATVSFIARDFEEDLKQQHIDLLRATAERVVGAHPPATVEVTDQPQYP
                     NMRDHLAPFPEVVANAEEAIRREGLEPVKTPIRGGTDGSQLSAKGLPTPNIFTGGHEY
                     HSVREWASVQEMAAAAAVLVHLAAVWTESGAV"
     gene            3321..>3506
                     /locus_tag="VFY44_02905"
     CDS             3321..>3506
                     /locus_tag="VFY44_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909415.1"
                     /translation="MSAHAHHHEHEDIEFSELDWTDPKRHAWLLGLLVPVIPFIAWGL
                     VEATGSGAFWFFGPVFLY"
BASE COUNT          521 a         1251 c         1252 g          482 t
ORIGIN      
        1 ttcggccgcc ggccgatctc ccgcgcgtgg ctggccgtgg tgttcccggc gctgaccctg
       61 aactacctcg gccagggcgc gctgctggtg aacgagccgt ccgcggtcag caacccgttc
      121 ttcctgctgg tccccgactg ggcgctcatc cccatggtcg cgctggccac gctggccacc
      181 gtgatcgcct cgcaggcggt catctccggc gcctactccg tctcgcacca ggccgcgcgg
      241 ctgggcttcc tgccccggct gagggtgctg cacacctcgg cccgcgagga gggccagatc
      301 tacgtgccgc tggtcaactg gctgctcatg gtcgccgtgc tggcgctggt gctcacgttc
      361 cagagctcgg agaagctggc cgccgcctac ggcgtcgcgg tcatcggcac catcaccgtg
      421 accaccacgc tgttcttcac gctccagtgg gtccgcgacc gccggccgca ctggcaggtg
      481 gcggcggtgg gcgcgctgtt cctggccttc gtgctggtgt tcctggcggc caacctggtc
      541 aagatcggcg agggcggcta cctgccggtg ggcatcggcc tggtcatctt caccgtgctc
      601 tccacgtggc ggcgcgggcg caagctggcc gacgccaacc gcgacgaggt cgagggcagc
      661 ctccaggagt tcgtcaacga cctgcacaac cacgagatcc ccgtgcgccg cgtgcccggg
      721 tgcgcggtct tcctcagccg cggcgagggc tcgacgccgc tggcgctgcg ggccaacgtg
      781 gagcacaacc acaccctgca ccgccacacc gtgatcctga cgctgctgag cctgcgcacc
      841 ccgcggatcg gcgccgagga ccgcgtcacg gtcgacgacc tgggcttcag cgacgacggc
      901 atcacccacg tgaccgcccg cttcggctac ctggagacga tgagcctgcc ctcggtgttg
      961 cggcaggcca gcgagaaggg cctggagggc ggcaaggtgg acgtggagaa ggccagctac
     1021 ttcgtctcgg cacccgagct gcgcatcacc gacgcgcccg gcatggccca gtggcgcaag
     1081 cacctgttcc tggccacgac ccgcctgacc tccgacccgg tcgagttctt cgacctaccg
     1141 cgcggcaaca cggtggtcat gggggcggag atcgatctgt aggtcgccct gccgtcagcc
     1201 gcaacagctg atcggcgaga actgcttggc tgggtagggc ccgggggcaa ccgcgcgtga
     1261 cggcttgttc ccgccccgcg ggcagaactc cacccgcggg gtcgatttct cccggatagc
     1321 gagagcaatc gaccgcgcgc tacccagccc cggccgttgg ccgagcggct ggtgcggccg
     1381 gcggcagaac cgctagcgct cgtcggcctt gcgcacgaag tgccacgccg acggcagcac
     1441 ggcggcgccg atggcggcct tgaccaggcc gcccaggatg aagatggtga agccgctgtg
     1501 gatggcgtcg ccccaggtca ggtcggctga gaccttgagc cacgggacgc cgatgccgaa
     1561 gatgatcagc tgcgccgcga cgaaggcggc gaaggcggtc aggacctttc ggtcggcgcc
     1621 gcgctcggcc attaggccga tcacgtaggt ggccaggatg aagccgatga tgtagccgcc
     1681 gctggcgccc cagatgacct cccagccgct cccgccgtcg gcgtagaccg gcaggaagag
     1741 gccgaggacc gcgtacaggc tcatcgcgct gaccccgcgg cgcgcgccca gggtggcgcc
     1801 gatcaggccc acgcccagcg tctggccggt gatcgggacg agctcgcccg gcatcttgat
     1861 ggtgacctgg gccatcagcg ccatgaaggc cgcgcccccg atcacgaggg cgatgtcgcg
     1921 ggccagggcg ccggggatgg cgtcggccag gacggagcgg cggggcagag cggcagacga
     1981 ggcggttgac atgggcgggc acaatacagc cgtgcccgac tccgctccct tcacctcgcc
     2041 cctggcccag cagctgtcgg aggaggtgac cgagcgcctg gtccgctacg cgcgcgtgga
     2101 cacgcagtcc agccgggagg gcaccggcac ccccagcacc gagtgccagt gggacctggc
     2161 gcgcctgctg gagggcgagc tggccgagct gggcctcgag gacatcgagc tcgacgagca
     2221 cggctacgtg ttcgccacgc tgcccgccac ggtggagggg acgccgacga tcgggctgct
     2281 ggcccacatc gacgtcagcc ccgacgctcc cgccgagggg gtggagccga tcgtgcaccg
     2341 cggctacgac ggcggcgtga tcgagctgcc caggggcggc acgaggctcg acccggccac
     2401 ggtgccgggc ctggccgagg cgaagggcca ggacctggtg acctccagcg gcgacacgct
     2461 cctgggcgcc gacgacaagg ccggcatggc cgaggtgatg accgccgtgg cccacctggc
     2521 ggccaacccc ggcctgccgc ggccggcgat ccgggtgggc ttcacccccg acgaggagat
     2581 cggcaagggg ccgatcctgt tcgacgtcga gcgcttcggg gcccggtgcg cgtacacgct
     2641 cgacggctcc gacctgggcg agatgcaggc cgagtcgttc accggctcgg aggtggtggt
     2701 gcgcatcaag ggcgtagacg tgcacccggg cagcgcgaag gacatcctga tcaacgccac
     2761 ccggctcgcg ggcgaggtcc tggccgcgct gcccgccgac ctgacgccgg agcgaaccga
     2821 gggccgccag ggcttcctgc acccggtcga gatcgccggc gacgccgtcg aggccaccgt
     2881 cagcttcatc gcgcgcgact tcgaggagga cctcaagcag cagcacatcg acctgctgcg
     2941 cgcgaccgcc gagcgggtcg tgggcgccca cccgcccgcc acggtcgagg tcaccgacca
     3001 gccccagtac ccgaacatgc gcgaccacct ggcgccgttc ccggaggtgg tggccaacgc
     3061 ggaggaggcc atccggcgcg agggccttga gccggtgaag acgcccatcc gcggcggcac
     3121 cgacggctcg cagctgagcg cgaaggggct gcccacgccc aacatcttca ccggcgggca
     3181 cgagtaccac tcggtgcgcg aatgggcctc ggtgcaggag atggcggccg cagccgccgt
     3241 gctcgtgcac ctggcggcgg tgtggacaga atcgggcgcg gtgtaactct ggttgacaaa
     3301 aggtggtacc gttgtcaacc atgagcgcac acgcccacca ccacgagcac gaggacatcg
     3361 agttcagcga gctggactgg accgacccca agcgccacgc ctggctgctg ggcctgctgg
     3421 tcccggtgat cccgttcatc gcctggggcc tggtcgaggc caccgggtcg ggcgcgttct
     3481 ggttcttcgg cccggtgttc ctgtac
//
LOCUS       DASUPW010000126         2868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1413803, whole genome shotgun sequence.
ACCESSION   DASUPW010000126 DASUPW010000000
VERSION     DASUPW010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413803"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VFY44_02910"
     CDS             complement(<1..448)
                     /locus_tag="VFY44_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909416.1"
                     /translation="MNIRTHIRRPRASLVIASLALFVALGGSATAATLITGAKVKNGT
                     ITGTDIKASSLTGAKVKNGSLSSSDLSKSAKGALKGAKGDQGPAGQTGPAGAQGAQGP
                     RGIVSPRVQTLGGKDINDGAAQTVLTSAVPAGTYVITAKATLVAEDN"
     gene            complement(498..1157)
                     /locus_tag="VFY44_02915"
     CDS             complement(498..1157)
                     /locus_tag="VFY44_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909417.1"
                     /translation="MNVRRHVRRPRASTLIASLALFVALGGSATAATLITGAQIKNGS
                     ITGKDVKASSVTGADVKNGALGSADFSRSTKASSKGERGTQGAAGQAGAPGGQGVPGP
                     NGVRAPERSNLVANINGGDIDSEILTKEVPPGQYLVTAKGMLFAGDAGHVTCRLRNGG
                     STVDRIDWDATAAGSARMPAQLQAVTNVTSGPLSLHCQKSGPVSGAFSSTQFIAIPTS
                     P"
     gene            complement(1144..1344)
                     /locus_tag="VFY44_02920"
     CDS             complement(1144..1344)
                     /locus_tag="VFY44_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909418.1"
                     /translation="MTSPLPIRFACQIDSLEEPIRGRLEDGDGRSVAFRGWIEFAVAI
                     TDMAREDAESTTTRGVSINERP"
     gene            complement(1448..>2868)
                     /locus_tag="VFY44_02925"
     CDS             complement(1448..>2868)
                     /locus_tag="VFY44_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5909419.1"
                     /translation="LKFAGDSVRAVEVLEEAAAALGDADAELAERLEVELIGSAYISV
                     SARRMLADRIDAFEDSGLPPETFLERFKLAGMCFDAFAEGRPAEEVARLATRALAGGD
                     LPTDPVAGGHAFVSAAIGLQFAEHYEEADRLYTGALEDARRWGSGVSFAAASSLRSLV
                     NYRRGRLSEAEADARAALDLAGDVHGSQGFLSAALGTLVYASLDRGTVDAELEQLAER
                     FLVEQATDNLPYTHALHSRACLLVERGELRRGLEELLAAGRRELEWGAVNPAITPWRS
                     SAALVHAALGERDQALALAAEEVELARAFGAPRCLGVALRALGVVQGGPEGQATLAEA
                     VEVLERSAAKLELARALVDLGAGIRVTGERREARAALSRGLELATECGAAALAERARN
                     ELLAAGARPRRTAVTGVGALTPSERRVAEMAAEGMVNREIAQALFVTEKTVETHLGKA
                     YAKLKVKSRKQLPEALGEATVA"
BASE COUNT          336 a         1083 c         1038 g          411 t
ORIGIN      
        1 cgttgtcctc ggccaccagc gtcgccttgg cggtgatcac gtaggtgccg gccggcaccg
       61 cgctggtcag cacggtctgg gcggctccgt cgttgatgtc cttcccgccc agggtctgga
      121 cccggggcga gacgatgccc cggggtccct gggcgccctg cgcgccggcc gggcccgtct
      181 gaccggccgg gccctggtcg cccttcgcgc ccttgagcgc gcccttcgcc gacttgctca
      241 ggtcggagga gctcagcgag ccgttcttga ccttggcgcc ggtaaggctc gaggccttga
      301 tgtccgtgcc ggtgatggtg ccgttcttga ccttggctcc ggtgatcagg gtggccgccg
      361 tggccgaccc gcccagcgct acgaacagcg cgagcgaggc gatcacgagc gaggcgcggg
      421 gacggcggat gtgggtacgg atgttcatgg ctttcacttc tccttggatg gtgtggtgac
      481 ggttggcgcg gcgctggtca ggggctggtg gggatcgcga tgaactgcgt cgaggagaac
      541 gccccgctga cgggaccgct cttctggcag tgcaggctga gcggaccgga cgtgacgttc
      601 gtgacggcct gcagctgggc cggcatccgt gccgagccgg cggcggtggc gtcccagtcg
      661 atccggtcca ccgtggagcc cccgttccgg aggcggcagg tgacatgccc ggcgtcgccg
      721 gcgaagagca tgcccttggc cgtgaccagg tactggccgg gcgggacctc cttcgtcagg
      781 atctccgagt cgatgtcgcc gccgttgatg ttggcgacga ggttcgaccg ctccggcgcc
      841 ctgacgccgt tcggcccggg cacgccctgg ccacccgggg caccggcctg gccggcagca
      901 ccctgggtgc cgcgctcgcc cttcgacgac gccttcgtgg acctgctgaa gtcggcggac
      961 cccagcgcgc cgttcttgac gtcggctccg gtcaccgacg aggccttcac gtccttgccg
     1021 gtgatgctgc cgttcttgat ctgggctccg gtgatcagcg tcgccgccgt cgccgaaccg
     1081 ccgagcgcga cgaagagcgc cagcgaggcg atcagcgtcg acgcgcgggg gcgcctgacg
     1141 tgtctacgga cgttcattga tgctcactcc tcgggtggtg gtggactcgg cgtcctcccg
     1201 ggccatgtcc gtgatcgcca ccgcgaactc gatccatccg cggaacgcca cggaccgccc
     1261 gtccccgtcc tccagccgcc cgcggatcgg ctcctcgagg gagtcgatct gacaggcgaa
     1321 ccggatgggc aggggggagg tcatgcggcc accctgccgc cgcgccaccc cccggtcgtg
     1381 agtgccgacc ccccgatgtc cccctgaggt ttcgcccccc gagacagggg gtgcgcgcct
     1441 cggggcccta ggccacggtg gcttcgccca gcgcctcggg cagctgcttg cgcgacttca
     1501 ccttgagctt ggcgtaggcc ttgcccaggt gggtctcgac cgtcttctcg gtcacgaaca
     1561 gcgcctgggc gatctcgcgg ttgaccatgc cctcggccgc catctcggcc acccggcgct
     1621 cgctgggggt cagcgccccg accccggtca cggccgtccg gcgcggccgt gcgccggcgg
     1681 ccagcagctc gttgcgggcc cgttcggcca gcgccgcggc gccgcactcg gtggccagct
     1741 ccaggccgcg gctcagcgcg gcgcgggcct cgcgccgctc gccggtcacc cggatccccg
     1801 cgcccaggtc caccagcgcc cgcgccagct ccagcttggc ggccgagcgc tccagcacct
     1861 cgacggcctc ggccagcgtg gcctgcccct cgggcccgcc ctggaccacg ccgagcgccc
     1921 gcagcgccac gccgaggcag cggggcgcgc cgaaggcgcg cgccagctcg acctcctcgg
     1981 cggccagcgc cagcgcctgg tcgcgctcgc ccagcgccgc gtgcaccagc gcggcgctcg
     2041 agcgccacgg cgtgatggcc gggttcaccg cgccccactc cagctcgcgc cggccggccg
     2101 ccagcagctc ctccaggccg cggcgtagct cgccgcgctc gaccagcagg cacgcgcggc
     2161 tgtgcagcgc gtgcgtgtag ggcaggttgt cggtggcctg ctcgaccagg aagcgctcgg
     2221 ccagctgctc gagctcggcg tccacggtgc cccggtccag cgaggcgtag accagggtgc
     2281 ccagcgcggc ggagaggaag ccctgggagc cgtgcacgtc gccggccagg tcaagcgcgg
     2341 cgcgggcgtc ggcctcggcc tcggacaggc ggccgcggcg gtagttcacc agcgagcgta
     2401 ggctggacgc ggcggcgaac gagacgcccg agccccaccg gcgcgcgtcc tccagcgcgc
     2461 ccgtgtacag gcggtcggcc tcctcgtagt gctcggcgaa ctggagcccg atggcggcgc
     2521 tcacgaaggc gtgcccgccc gcgacggggt cggtcggcag atcgccgccg gccagcgcgc
     2581 gggtggccag ccgcgccacc tcctcggcgg gacggccctc ggcgaacgcg tcgaagcaca
     2641 tgccggcgag cttgaagcgc tccaggaacg tctccggcgg gagcccggag tcctcgaacg
     2701 cgtcgatccg gtcggccagc atccggcggg cgctgacgct gatgtacgcg ctcccgatca
     2761 gctcgacctc cagccgctcg gccagctcgg cgtcggcgtc gcccagcgcg gccgcggcct
     2821 cctccagcac ctcgaccgcg cggaccgagt caccggcgaa cttcagca
//
LOCUS       DASUPW010000127         2289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_167999, whole genome shotgun sequence.
ACCESSION   DASUPW010000127 DASUPW010000000
VERSION     DASUPW010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167999"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VFY44_02930"
     CDS             complement(<1..521)
                     /locus_tag="VFY44_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909420.1"
                     /translation="MKRYVVVWLKLLRLSWQLFPGQTAGLLVLRLLMVGVTAATALAM
                     RAVVNAVVEGDPAGAVRASAVTALTLGLTAYALGAEHNVLFLLVERVGLKHVRAQIDR
                     DIAGLEGLDHLERTDFLDRVTVVRGAAWGLTYGMWNAVDTIFAALRLVVMLVLLGVVN
                     PWLLLLLAFAAAPL"
     gene            complement(518..>2289)
                     /locus_tag="VFY44_02935"
     CDS             complement(518..>2289)
                     /locus_tag="VFY44_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5909421.1"
                     /translation="TISALAMLGSLVPAATALAVAFLVGRVEAATADAVLSASVLPLG
                     LFAGVLIAGQLMEAVRSPLGGLAKARIDGAHRRHIIVLTTGGSTLDVLESPRVQQLIR
                     EAKADPDNWTQRTPAQGALAQLDLLGSLVGLMGSFAVLASYSWWLVVMLAVPLLVARM
                     LDTAGERAGYRTWLKGSGEGMHAGKWEEAIVSPGEGKEARVFGLGDWVVARMQHHIGA
                     MFEPVWAVHRRNMALRWRQALLLAVPAGIAFAMVADSAASGFTSVAVVTAVLSAGASL
                     HMILDPFSDPSIIGGVACLRATQQLRVELGGAKPQASEPAHAAPSVPPSAPLVRFEAV
                     SFTYPRTGRVVLQHVDLEIRPGELLAIVGLNGAGKSTLIKLLAGLYTPSSGRITADGV
                     DLARLGIPAWRQRIAVVFQDFVRYHLSAEDNVVLGRPDRDPAALDAAASDAGLDELIA
                     RLPDGWRTPLARSRTGGVDLSGGQWQQVVLARALYAVRTGARLLVLDEPTAHLDVRTE
                     FDVFERLAAHKGDASVVLISHRLSTVRRADRIVLLEHGRIAESGTHDELMTLGGSYAE
                     MFQIQADRFQRGFDDRIEEGELL"
BASE COUNT          377 a          839 c          781 g          292 t
ORIGIN      
        1 agcggggccg ctgcgaacgc cagcaggagc agcagccagg ggttgacgac gccgagcagc
       61 accagcatga cgacgagccg cagcgcggcg aagatggtgt cgacggcgtt ccacatgccg
      121 tacgtcaggc cccacgccgc gccccggacc acggtgacgc ggtcgaggaa gtcggtacgc
      181 tcgaggtgat ccagcccttc cagcccggcg atgtcgcggt cgatctgggc ccggacgtgc
      241 ttcaagccga cccgctcgac caacaggaag agcacattgt gctcggcccc gagagcgtac
      301 gcggtcagcc cgagcgtcag cgccgtgacg gcactggccc gtacggcgcc ggccgggtcg
      361 ccctcgacca ccgcgttgac cacggcccgc atggcgagcg cggtcgccgc ggtgacgccc
      421 accatcagca gccgcaacac cagcagccct gcggtctggc ccggaaaaag ttgccaggat
      481 aggcgcagca gcttcagcca taccacgacg taccgcttca cagcagctcc ccttcctcga
      541 tccggtcgtc gaagccgcgc tgaaaccggt cggcctggat ctggaacatc tcggcgtacg
      601 acccgcccag ggtcatgagc tcgtcgtgcg tcccggactc cgctatgcgg ccgtgctcca
      661 gcagcacgat ccggtcggcc cgacgcaccg tggagagccg gtgggagatc agcaccacgc
      721 tggcgtcgcc cttgtgcgcg gcgaggcgct cgaacacgtc gaactccgtc cgcacgtcca
      781 ggtgggcggt gggctcatcc agcacgagca ggcgggcgcc ggtgcgcacc gcgtacagcg
      841 cccgggccag gacgacctgc tgccactggc cgccggacag gtcaaccccg ccggtacgcg
      901 accgggccag cggtgtgcgc caaccatccg ggagacgggc gatcagctca tcgagcccgg
      961 cgtcgctggc ggccgcgtcc agggccgccg gatctctgtc gggccggccc agcaccacgt
     1021 tgtcctccgc cgacaggtgg tagcggacga agtcctggaa caccaccgcg atccgttggc
     1081 gccaggctgg aatgccaagg cgtgccaggt ccacgccgtc cgcggtgatc cggccggagg
     1141 acggagtgta gaggccggcc agcagcttga tcagagtcga cttgccggcg ccgttgaggc
     1201 ccacgatggc cagcagctcg ccggggcgga tctcgaggtc gacgtgttgc agcacgacgc
     1261 ggccggtccg tgggtacgtg aaggacaccg cttcgaacct caccagcggc gcggagggcg
     1321 ggacagacgg tgctgcgtgg gcgggttcgc tcgcctgcgg ctttgcgccg cccagctcga
     1381 cccgcagctg ctgcgttgcc cgcaggcaag caaccccgcc gatgatgctg gggtcgctga
     1441 acggatccaa gatcatatgt agcgacgcgc ccgcgctcaa caccgccgtg accacggcga
     1501 cggaggtgaa cccgctcgcc gccgagtcgg cgaccatggc gaatgcgatc cccgccggga
     1561 cggccaacag cagcgcctgc cgccagcgca gggccatgtt gcgccggtgg accgcccaca
     1621 ccggctcgaa catcgccccg atgtggtgct gcatgcgggc gacgacccag tcgcccaggc
     1681 cgaacacgcg ggcctccttg ccctcgcccg gcgagacgat cgcctcctcc cacttgcccg
     1741 cgtgcatgcc ctcaccgctg cccttgagcc acgtccggta cccggcgcgt tcgccggcgg
     1801 tgtccagcat ccgcgccacc agcagcggca cggcgagcat caccaccagc caccacgaat
     1861 agctggccag caccgcgaac gagcccatca acccgacgag ggagccgagc aggtcgagct
     1921 gggccagcgc cccctgggcg ggggtgcgtt gcgtccagtt gtccgggtcg gccttcgcct
     1981 ccctgatgag ctgctggacc cgcgggctct ccaggacgtc caaggtggac cccccggtag
     2041 tgagcacgat gatgtgccgt cgatgcgcgc cgtcgatgcg cgccttcgcc agaccgccca
     2101 ggggactacg gaccgcctcc atcagttgac ctgcgatcag cacgccggcg aagagcccga
     2161 gcggcaggac agatgcggac agcacggcgt cggcagtggc cgcctcgacc cgtccgacga
     2221 ggaaggccac cgcgagcgcg gtggcggccg gcaccagact gcccagcatc gccagggcgc
     2281 tgattgtca
//
LOCUS       DASUPW010000128         3240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_307297, whole genome shotgun sequence.
ACCESSION   DASUPW010000128 DASUPW010000000
VERSION     DASUPW010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307297"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VFY44_02940"
     CDS             complement(<1..664)
                     /locus_tag="VFY44_02940"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HEX5909422.1"
                     /translation="MTRSGAVMASQATSSRPRTVPATAWARLRSSAAFADSWRALVWS
                     RLGIFCVALYATLAGGLSSGNAAKFDTPSLTHPFGGLGDLLFTPLARWDSVWYLSIAN
                     LGYGGDDSPRTAFFPLFPLLSRGVGELGGGSEAAVLVGSYVVALAALGVALYLLHRLT
                     ELELGRAAAGPAVLLLCAFPASVFLGAPYSESLFLAASVGAFYAARTGHWAAAGAAAA
                     AAS"
     gene            642..1439
                     /locus_tag="VFY44_02945"
     CDS             642..1439
                     /locus_tag="VFY44_02945"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX5909423.1"
                     /translation="MTAPDLVIAASFVAEHRETITAIATVVVAIVIAQIVDRALSHSG
                     QELREGVAGRSISAVADTRLRLVRRLIFFLIIVIGIALALAQFPAVKRVATGLLASSA
                     LLGLVVGFAARQTLANVIAGIVIAITQPIRVGDLVTFEDETGEVEDIRLAYTYIRLDN
                     GQRLIVPNESLAQSSVHNHTVVDPRVKVEIEVWLPPGGDATRALELLAADEGVDARVS
                     EVDHEGITLVVASWAENARERGGVAARLRKESLERLRDAGLSSVPTG"
     gene            1518..>3240
                     /locus_tag="VFY44_02950"
     CDS             1518..>3240
                     /locus_tag="VFY44_02950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX5909424.1"
                     /translation="MTYRQRKQRRRRRRGGAGQRLLILFGIVGAVIAVAGLSVTGYVL
                     AIAATAPDLSELKPADKGSNSEILAADGSRLGYVQSDEIRTPLPWAEMPSDMRQAVVA
                     IEDERFYSHKGVDYGGIVRAGVKNLESGKTTQGGSTITQQLVRALYIKDPQRNVQRKI
                     REAKLASELEKKHSKTWILKNYLNDVPFGTVEGRTAIGIEAAAETFFNKHARELTLPE
                     AALLAGLPQAPSQYNPFRNPRAALQRRAEVLKRMVKNDFITQAQADDAAQAPLTLKRG
                     RRYTDRREPFFFDFVQEQLIERYGVGVYRRGGLKVFTTINPKQQDVARNAINQFYGDT
                     SGPASAIASIDPHNGEIKAMASSGTYKDRRFNLAAQGHRQPGSAFKTMVLTAAIRSGI
                     NPTSTTYTSKPLNLNVPGYGPWQVKTFSNSYGGTMNLVRATTSSDNSVYAQLIIDVGP
                     KKVCETAKLLGIETKLDCLPAEGLGGLRLGVTPLEIANAYATLASGGVRHKPTGIRKV
                     EFPDGKSENLGNSKGKRVLTDGQAYEVTKILKANVTGGTGTRAQFGCPAAGKTGTTDS
                     SKDAWFVG"
BASE COUNT          557 a         1166 c         1132 g          385 t
ORIGIN      
        1 cggacgccgc ggccgccgcc gcgccggccg ccgcccagtg gccggtgcgg gcggcgtaga
       61 acgcgccgac ggacgcggcc aggaacaggc tctcggagta cggcgcgccc aggaacaccg
      121 acgcgggaaa cgcgcacagc agcagcacgg cggggccggc cgcggcgcgg cccagctcga
      181 gctcggtcag ccggtgcagc aggtagagcg cgacgcccag ggcggccagg gccacaacgt
      241 acgagcccac cagcacagcg gcctccgagc cgccgcccag ctcgcccacg ccgcgcgaga
      301 gcagcgggaa cagggggaag aacgcggtcc gcggcgagtc gtcgccgccg tagcccaggt
      361 tggcgatcga caggtaccag accgagtccc agcgggccag gggcgtgaac agcaggtcgc
      421 ccaggccacc gaacgggtgg gtcagcgacg gcgtgtcgaa cttggccgcg ttgccggagg
      481 acagcccgcc ggcgagcgtc gcgtagaggg cgacgcagaa gatgcccagc cggctccaga
      541 ccagggcgcg ccaggagtcg gcgaaggccg ccgacgagcg gagccgggcc cacgccgttg
      601 cagggacggt gcggggccgg ctggaagtag cctgcgacgc catgaccgcc cccgatcttg
      661 tcatcgccgc ctcgttcgtg gccgagcatc gcgagacgat caccgccatc gcgacggtgg
      721 tggtggccat cgtgatcgcc cagatcgtgg accgcgcgct gtcgcacagc ggccaggagc
      781 tgcgcgaggg cgtggccggc cggtcgatct cggcggtggc cgacacccgg ctgcggctgg
      841 tgcggcggct gatcttcttc ctgatcatcg tgatcgggat cgcgctggcg ctggcgcagt
      901 tccccgcggt caagcgggtg gccaccgggc tgctggcctc gtcggcgctg ctgggcctgg
      961 tcgtgggctt cgcggcgcgg cagacgctgg ccaacgtgat cgcgggcatc gtcatcgcca
     1021 tcacgcagcc gatccgcgtc ggggacctgg tcacgttcga ggacgagacc ggcgaggtgg
     1081 aggacatccg cctggcctac acgtacatcc gcctggacaa cggccagcgg ctgatcgtgc
     1141 ccaacgagtc gctggcccag agctcggtgc acaaccacac cgtcgtcgac ccgcgcgtga
     1201 aggtggagat cgaggtctgg ctgccccctg gcggggacgc cacgcgcgcg ctggagctgc
     1261 tggccgccga cgagggcgtc gacgcgcgcg tgagcgaggt cgaccacgag ggcatcacgc
     1321 tggtggtggc cagctgggcg gaaaacgccc gcgagcgggg aggagtggcg gcgcgcctgc
     1381 gaaaagaaag cctcgagcgc ctgcgcgacg ctgggctatc ctccgttccg accggctgag
     1441 gccctagcgc gcacgggcgc gcgtggaaag gctgtgtgat ccaccacggg ccccgcagcg
     1501 caaaccggtc gccacccatg acctaccgcc agagaaagca gcgtcgacgt cgccgccgtg
     1561 gaggagccgg tcagcgcctg ctgatcctgt tcgggatcgt cggcgcggtg atcgccgtcg
     1621 ccggcctgtc ggtaaccggc tacgtgctgg ccatcgccgc aaccgcgccc gacctgtccg
     1681 agctcaagcc ggccgacaag ggctcgaact cggagatcct tgccgccgac gggtcgcggc
     1741 tgggctacgt ccagtcggac gagatccgca cgcccctgcc ctgggcggag atgccctccg
     1801 acatgcgcca ggccgtggtt gcgatcgagg acgagcgctt ctacagccac aagggcgtgg
     1861 actacggcgg catcgtccgc gccggcgtca agaacctgga gtccggcaag accacccagg
     1921 gcggctcgac catcacccag cagctggtgc gcgcgctgta catcaaggac ccccagcgca
     1981 acgtgcagcg caagatccgc gaggccaagc tggcctcgga gctggagaag aagcactcca
     2041 agacctggat cctcaagaac tacctcaacg acgtgccgtt cggcaccgtc gaggggcgta
     2101 cggccatcgg gatcgaggcc gccgccgaga ccttcttcaa caagcacgcc cgcgagctga
     2161 ccctgcccga ggcggcgctg ctggccggcc tgccccaggc cccgtcgcag tacaacccgt
     2221 tccgcaaccc gcgcgccgcg ctccagcgcc gggccgaggt gctgaagcgg atggtcaaga
     2281 acgacttcat cacccaggcg caggcggacg acgccgcgca ggcgccgctg accctcaagc
     2341 gcggccgccg ctacaccgac cggcgcgagc ccttcttctt cgacttcgtg caggagcagc
     2401 tgatcgagcg ctacggcgtg ggcgtctacc gccgcggcgg gctcaaggtc ttcaccacga
     2461 tcaaccccaa gcagcaggac gtggcgcgca acgccatcaa ccagttctac ggcgacacca
     2521 gcggccccgc gtcggcgatc gcgtcgatcg acccgcacaa cggcgagatc aaggccatgg
     2581 cctcgagcgg cacctacaag gaccggcgct tcaacctggc ggcccagggc catcgccagc
     2641 ccggctcggc cttcaagacg atggtgctga cggcggcgat ccggtcgggc atcaacccca
     2701 ccagcaccac ctacacctcc aagccgctga acctgaacgt gcccggctac gggccgtggc
     2761 aggtcaagac cttcagcaac agctacggcg gcacgatgaa cctggtccgc gccacgacct
     2821 cgtccgacaa ctcggtgtac gcgcagctga tcatcgacgt cggcccgaag aaggtctgcg
     2881 agaccgccaa gctgctgggc atcgagacca agctcgactg cctgccggcc gagggcctcg
     2941 gcggcctgcg cctgggcgtg accccgctgg agatcgccaa cgcctacgcc accctggcca
     3001 gcggcggcgt ccgccacaag cccaccggca tccgcaaggt cgagttcccc gacggcaagt
     3061 ccgagaacct gggcaactcc aagggcaagc gcgtgctcac cgacggccag gcctacgagg
     3121 tcaccaagat cctgaaggcc aacgtgaccg gcggcaccgg cacccgggcc cagttcggct
     3181 gcccggcggc gggcaagacg ggcaccaccg acagctcgaa ggacgcgtgg ttcgtgggct
//
LOCUS       DASUPW010000129         4331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1691755, whole genome shotgun sequence.
ACCESSION   DASUPW010000129 DASUPW010000000
VERSION     DASUPW010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691755"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1371)
                     /locus_tag="VFY44_02955"
     CDS             complement(73..1371)
                     /locus_tag="VFY44_02955"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HEX5909425.1"
                     /translation="MNPPGAQGRSWGLRFRMRERLDRSWLVIPSLYVVAALVLGKAVP
                     ALESGHRIDPFALALSTDSAREILNAVAGGMIAFTGLVVSVAVVVVQFGAGQYTPRLV
                     LRFRRDLVVKHALGIFIAPALYALVALAQLGSGADLEPNVTVGLAVVLLVAAVLAFFA
                     LVARLLDLLRPRRLFEQLLAGCEQAIDQVYPRAYEGRADPVEEEVPPVTATVAHTGSE
                     GVVSALDLPLLMTLATEADAVIEAGPVVGSFLWRGAPLAHVRGTVREVPEPDLERAII
                     VSDERTLAQDPAFAIRAIVDIAIRALSPAVNDPTTACQALDTLESLLHRLAGRELGPG
                     RLHDAGGALRVLYRAPGWDELVTLALTEIRHYGASSHQTARRMRALLEGLAPVVPAER
                     AGAVREQLALLDAAVAAAFPDPAERAVASAADHMGLGGVG"
     gene            1394..2566
                     /locus_tag="VFY44_02960"
     CDS             1394..2566
                     /locus_tag="VFY44_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5909426.1"
                     /translation="MDPIANAAQLTPERLEATRAASAELVRATPVLTTRSLSERCGGT
                     IALKAENLQRTGSFKLRGALSKVRALAGKAEGVVAGSAGNHAQALAYAARSSGLRCEV
                     FVPDDAPLSKVAAIRSFGAQVERGGPAVGDCLASARDAAGNRGLTFVSPYDDWDVIAG
                     QAGVGLELAADLPDLARVVVPVGGGGLASGVALALPGVEIVGVRAANPLQTIADGIAV
                     KGHGEITGPLLDEHLSGLVTVGEEHIAEAMVFLLERSKLLVEGAGAVGVAALMAGAVE
                     PAPTGTTVAILSGGNIDLALVAALAARQETAAGRRIRLFTRLRDRPGGLAKLLAEIAA
                     QNANLIQIEHVRDGIDLAVRETGVELTLETDGADHAVAIVDHLADEGYSIRVVSTD"
     gene            complement(2536..3528)
                     /locus_tag="VFY44_02965"
     CDS             complement(2536..3528)
                     /locus_tag="VFY44_02965"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEX5909427.1"
                     /translation="MTLTSGRLLRLLSLLQARRYWPGDELADRLEVSGRTIRRDVERL
                     RELGYPVDATTGPDGGYRLEAGAAMPPLLLDDDEAVAIAVGLRTAAGASVSGIEETSV
                     RALVKLEQVLPPHLRRRVNALQSATATLTQGSGGPQVHPEALTVIAGACRDRERLRFA
                     YKARDGAETRRHAEPLSLVNLGRRWYLVAWDCDRRDWRTFRLDRIPKLPTAGGRFEPR
                     TLPDGMDPATYVSKNLSGVQYRHQARVTLHAPAAEVEAGHRWARGQVEPIDEGSCSYA
                     PSDDSLEWLALRIAFLGVDFEVHEPPELAEQCAALSRRFGAAAGAQSVETTRIE"
     gene            3592..>4331
                     /locus_tag="VFY44_02970"
     CDS             3592..>4331
                     /locus_tag="VFY44_02970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5909428.1"
                     /translation="MPITATIPDAVSVDTAERRSSGREPAIKVSGLVKTYGDVRAVDG
                     VYFEVTPGTVLGLLGPNGAGKTTTVRALTTLLKPDSGQMLVDGIDAVAEPARLREHIG
                     LAGQYASVDESLTGKENLVMVGRLYGMKRKVASARAVELLEQFDLSDASDRMVKKYSG
                     GMRRRLDLAAALVAKPPVLFLDEPTTGLDPRSRLDLWAMIEQLVAEGTTVLLTTQYLD
                     EADRLADRIAVIDRGKVIAEGTGDQLKER"
BASE COUNT          601 a         1596 c         1621 g          513 t
ORIGIN      
        1 atgggcgttc gcaacggcga ccgccggcgc cctggcgctc tgggccgtgc tggtcgcggt
       61 cgccggccgg ggctagccca ccccgccgag gcccatgtgg tcggccgcgg aggccaccgc
      121 ccgttcggcc gggtccggga acgccgcggc caccgccgca tcgaggagag ccagctgctc
      181 ccgcaccgct ccggcgcgct cggccggcac gaccggtgcc agcccctcca gcagcgcccg
      241 catccgccgc gccgtctggt gcgacgaggc gccgtagtgg cggatctccg tgagcgcgag
      301 cgtcacgagc tcgtcccatc ccggcgcccg gtagagcacc ctgagcgccc cgccggcgtc
      361 gtgcagccgg cccgggccca gctcgcgccc ggccagccgg tgcaggagtg actcgagggt
      421 gtccagcgcc tggcaggcgg tggtcgggtc gttgaccgcc ggcgacaacg cccggatggc
      481 gatgtcgacg atggcccgga tcgcgaacgc cgggtcctgg gccagcgtgc gctcgtcgga
      541 gacgatgatg gcgcgctcca gatcgggctc cggaacctcc cggaccgtgc cgcggacgtg
      601 cgccagcggg gcgccgcgcc agaggaagct gccgaccacg gggcccgcct cgatgaccgc
      661 gtccgcctcg gtggccagcg tcatcagcag aggcaggtcc agcgccgaca ccaccccctc
      721 ggaaccggtg tgcgcgacgg tggcggtgac gggcggcacc tcctcctcga cggggtcggc
      781 ccgcccctcg tacgcgcgcg gatagacctg gtcgatggcc tgctcgcacc cggccagcag
      841 ctgctcgaac agccgtcgcg ggcgcagcag gtccagcagc cttgcgacca gggcgaagaa
      901 ggccagcacc gccgccacga ggaggacgac ggccagcccg accgtgacgt tgggctccag
      961 gtccgccccg ctgcccagct gggccagcgc gaccagcgcg tagagcgccg gggcgatgaa
     1021 gatccccagc gcgtgcttga ccaccaggtc gcgccggaag cgcagcacca gccgcggcgt
     1081 gtactgcccg gcgccgaact ggaccacgac cacggcgacc gagaccacga ggccggtgaa
     1141 ggcgatcatg cccccggcga cggcgttgag gatctcgcgc gcgctgtcgg tggacagcgc
     1201 cagcgcgaac gggtcgatcc ggtgcccgga ctccagcgcc ggcaccgcct tgcccagcac
     1261 cagggcggcc accacgtaca gcgacgggat gaccagccag ctgcggtcca gccgctcgcg
     1321 catccggaag cgaaggcccc acgaccgccc ctgggctccc ggcgggttca caccggcatc
     1381 ctacgatggc tgcatggacc cgatcgccaa cgcggcccag ctcacacccg agcggctcga
     1441 ggcgacccgg gccgccagcg ccgagctggt ccgcgcgacg ccggtgctga ccacgcggag
     1501 cctgtcggag cggtgtggcg gcacgatcgc gctgaaggcc gagaacctcc agcggaccgg
     1561 gtcgttcaag ctccgcggcg cgctgagcaa ggtgcgcgcg ctggccggga aggccgaggg
     1621 ggtcgtggcc ggcagcgcgg gcaaccacgc ccaggcgctg gcgtacgcgg cgcgatcgag
     1681 cgggctgcgc tgcgaggtgt tcgtgcccga cgacgcgccg ctgtcgaagg tggccgcgat
     1741 ccggtcgttc ggcgcgcagg tcgagcgcgg cggcccggcg gtcggggact gcctggccag
     1801 cgcccgggac gcggccggca accgcggcct gacgttcgtc agcccctacg acgactggga
     1861 tgtgatcgcc ggccaggccg gcgtgggcct ggagctggcc gccgacctcc ccgacctggc
     1921 ccgggtcgtg gtgccggtgg gcggcggggg gctcgcatcg ggcgtcgccc tggccctgcc
     1981 gggggtcgag atcgtcggcg tgcgcgcggc caacccgctg cagacgatcg ccgacggcat
     2041 cgcggtcaag ggccacggcg agatcaccgg cccattgctc gacgagcacc tgagcggcct
     2101 ggtgacggtc ggcgaggagc acatcgccga ggcgatggtg ttcctgctcg agcgctcgaa
     2161 gctgctggtc gagggggccg gcgcggtggg cgtggccgcc ctgatggccg gcgcggtgga
     2221 gccggcgccc acgggcacga cggtggcgat cctgtccggc ggcaacatcg acctggcgct
     2281 ggtggcggcg ctggccgccc gccaggagac ggcggccggc cgccgcatcc ggctgttcac
     2341 ccgcctgcgc gaccgccccg gcgggctggc caagctgctg gccgagatcg ccgcgcagaa
     2401 cgccaacctg atccagatcg agcacgtgcg cgacggcatc gacctggcgg tgcgcgagac
     2461 cggcgtggag ctgacgctgg agaccgacgg cgccgaccac gcggtggcga tcgtcgacca
     2521 cctggccgac gagggctact cgatccgcgt ggtctccacc gactgagcgc ccgccgcggc
     2581 cccgaagcgg cgcgagagcg ccgcgcactg ctcggccagc tcgggcggct cgtggacctc
     2641 gaagtccacg cccaggaacg cgatccgcag cgccagccac tccagcgagt catcgctggg
     2701 cgcgtagctg cacgagccct cgtcgatcgg ctcgacctgg ccccgcgccc accggtggcc
     2761 ggcctcgacc tccgcggcgg gcgcgtgcaa ggtgacgcgc gcctggtggc ggtactggac
     2821 ccccgagagg ttcttgctga cgtacgtggc cgggtccatc ccgtcgggca gcgtccgcgg
     2881 ctcgaagcgg ccgccggccg tgggcagctt ggggatccgg tccaggcgga aggtgcgcca
     2941 gtcccggcgg tcgcagtccc aggccaccag gtaccagcgc cggcccaggt tgaccaggct
     3001 cagcggctcg gcgtggcgcc gcgtctccgc gccgtcgcgg gccttgtagg cgaagcgaag
     3061 ccgctcgcgg tcccggcacg cgcccgcgat gaccgtgagc gcctcggggt gcacctgggg
     3121 cccgccgctg ccctgggtca gcgtggcggt ggccgactgg agcgcgttca cccgccggcg
     3181 caggtgcggc ggcagcacct gctcgagctt gaccagcgcc cgcaccgacg tctcctcgat
     3241 cccggacacc gacgccccgg cggcggtgcg cagcccgacc gcgatggcca ccgcctcgtc
     3301 gtcgtccagc agcagcggcg gcatggcggc gcccgcctcc agccggtagc cgccgtcggg
     3361 cccggtggtg gcgtcgaccg gatagcccag ctcgcgcagg cgctcgacgt cgcggcggat
     3421 ggtgcgcccg gagacctcga gccggtcggc cagctcgtcg ccgggccagt agcggcgggc
     3481 ctggaggagc gagagcaggc gcaggaggcg tcccgaggtc agcgtcatgt gaagcaggat
     3541 tccatctatt gcggccagat actggcctaa acccgtcgta cggttagccg catgccaatc
     3601 accgcgacga tcccggatgc tgtgtcggtg gacaccgcag agagacgatc atcaggccgg
     3661 gagcccgcca tcaaggtgtc gggactcgtg aagacctacg gcgacgtgcg cgccgtggac
     3721 ggcgtgtact tcgaggtcac gcccggcacc gtgctgggcc tcctcggccc caacggcgcc
     3781 ggcaagacca cgaccgtccg cgcgctgacg acgctgctca agcccgactc gggccagatg
     3841 ctggtcgacg gcatcgatgc cgtggccgag ccggccaggc tgcgcgagca catcggcctc
     3901 gccgggcagt acgcatcggt cgacgagagc ctgaccggca aggagaacct ggtcatggtc
     3961 gggcgcctgt acgggatgaa gcgcaaggtc gcgtccgcgc gcgccgtcga gctgctggag
     4021 cagttcgacc tctccgacgc ctccgaccgg atggtcaaga agtactcggg cggcatgcgc
     4081 cgccggctgg acctggccgc ggcgctggtg gccaagccgc cggtgctgtt cctggacgag
     4141 ccgaccaccg gcctggaccc gcgcagccgg ctggacctgt gggccatgat cgagcagctg
     4201 gtggccgagg gcaccacggt gctcctgacc acccagtacc tggacgaggc cgaccggctg
     4261 gccgaccgca tcgccgtgat cgaccgcggc aaggtcatcg ccgagggcac gggcgaccag
     4321 ctgaaggagc g
//
LOCUS       DASUPW010000130         3849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_446204, whole genome shotgun sequence.
ACCESSION   DASUPW010000130 DASUPW010000000
VERSION     DASUPW010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446204"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..620)
                     /locus_tag="VFY44_02975"
     CDS             complement(162..620)
                     /locus_tag="VFY44_02975"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5909429.1"
                     /translation="MSRTVEAAGRVTLQPDEAAALWRDPRRWSAFVEGFQRIEELGAD
                     WPEQGAKLVWDSGPGGRGRVTEKVADNGPTRFATKVYEDRLYGTQTAVFTPDAGGGAE
                     VRLTLQYELTGDNPFNAVTDLLFIRRALRDALGRTLRRFAVEAQEEAGLR"
     gene            complement(617..1072)
                     /locus_tag="VFY44_02980"
     CDS             complement(617..1072)
                     /locus_tag="VFY44_02980"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX5909430.1"
                     /translation="MAERSKPERSRQAAREAVAQAGHPTRAPRQPRQGQDPFSGEMRR
                     RQVVPLLVFHLIAQGPSYGNQLMERIGELTEGVLSVNPNTMYPLLRKLEEDGMIEGQW
                     EHPERRSRRFYSLTRKGRSEYKRLLEDVRPLLESVKHSVDGILDEVYDT"
     gene            complement(1100..1939)
                     /locus_tag="VFY44_02985"
     CDS             complement(1100..1939)
                     /locus_tag="VFY44_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5909431.1"
                     /translation="MKGPVLITGCSTGIGRATAERLAAAGHTVYATARKPEAVADLEA
                     KGCRTLALDVNDEESMRAAVAAVEEADGPVGALVNNAGYSQSGAVESVNIDDIRAQFQ
                     TNVFGLIRMCQLVLPGMREAGRGRIVNVGSMGGKLTFPGGGIYHATKYSVEALSDALR
                     FEVKGFGVDVVLIEPGLIVSDFASAATASIDAGTAGDGPYADFNRAVGKATAGAYEGP
                     TAKLGGPPEAVAKVIEKALTTRRPRPRYTVTPSARIIMGQRKLLSDRMWDRFVGTQFP
                     RPS"
     gene            complement(1936..2934)
                     /locus_tag="VFY44_02990"
     CDS             complement(1936..2934)
                     /locus_tag="VFY44_02990"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5909432.1"
                     /translation="MRSVVITKAGPPEVLQVQERPDPRPGPGQVRVEVGAAGVNFADL
                     MARVGLYPDAPDLPAVVGYEVAGIVAELGEGVDRYTVGERVMAGTRFGGYSEQVVANV
                     TDTAPLPESLSFEEGAAVPVNYLTAYLGLRRFGALEPGERVFLHAAAGGVGIAATQIA
                     KHRGAEVWGSASPGKHDAIRGFGVDHAVDYTRSGWEKGLPPMDVVMDALGGKSFRRSY
                     DMLRAGGRLIAFGAAGVVEGDKRNLIAAGRTALTMPRFNLIKQMSASKSVIGLNMLTI
                     WDELGSLEPFMAAIEDLLDQGVLEPVVAASFPFDRAGEAHRYIGERRNIGKVVLTP"
     gene            complement(3056..>3849)
                     /locus_tag="VFY44_02995"
     CDS             complement(3056..>3849)
                     /locus_tag="VFY44_02995"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5909433.1"
                     /translation="EPDAGSDVSGMKTTAVKKGDKYVINGSKCFITNGSYASFYTVYA
                     KTDKEAGHRGISAFIVDRDLPGVTVDKKEDKLGQRASNTATISFNDVEIPAENMLGEE
                     NKGFKLAMMTLDRTRPGVAAMATGISRAAFEFATEYSKERVQFGVPIAMHQAIQFMIA
                     DMATKVHLSRIATWNSAVLLDQGKRNTLESSHAKRFAADSAMEVTTDAVQVYGGYGFI
                     KEYPVEKLMRDAKIMQLYEGTSQIQRLVIARETLMPRRAETPAAA"
BASE COUNT          501 a         1389 c         1367 g          592 t
ORIGIN      
        1 ccaccttcat gcccttggcc gaacaggccc gggcggtggc caggccgatc ccgcgtgcgg
       61 cgccggtgat ggcgacgacc ttccctcgta gctggcgggg ttccttcttg gccaaggcgt
      121 gctcctgggg ttcgggggaa actggctggg cagccaggac gctaccggag tccggcttct
      181 tcctgggcct cgaccgcgaa ccggcgcagc gtgcggccca gcgcgtcgcg gagcgcgcgc
      241 cggatgaaca acaggtcggt cacggcgttg aacgggttgt cgccggtgag ctcgtactgc
      301 agggtcaggc gcacctcggc cccaccgccc gcgtccggcg tgaacaccgc cgtctgggtg
      361 ccgtacaggc ggtcctcgta gaccttggtg gcgaaccggg tgggcccgtt gtcggccacc
      421 ttctcggtga cccggccgcg gccgcccggg ccggagtccc agacgagctt ggccccctgc
      481 tcgggccagt ccgcgcccag ctcctcgatc cgctggaagc cctccacgaa ggccgaccac
      541 cggcgcgggt cgcgccacag ggcggcggcc tcgtcgggct ggagggtcac gcggccggcc
      601 gcctccacgg tccggctcac gtgtcgtaga cctcgtccag gatgccgtcg accgagtgct
      661 tgacgctctc caggagcggg cgcacgtcct cgagcaggcg cttgtactcg gaccgcccct
      721 tgcgggtcag cgagtagaac cggcgcgagc gccgctcggg gtgctcccac tgaccctcga
      781 tcatcccgtc ctcctcgagc ttgcgcagca gcgggtacat ggtgttgggg ttcaccgaca
      841 gcaccccctc ggtcagctcg ccgatgcgct ccatgagctg gttgccgtac gacgggccct
      901 gggcgatcag gtggaagacc agcagcggca ccacctggcg ccggcgcatc tcgccgctga
      961 aggggtcctg gccctggcgc ggctggcgcg gtgcccgggt gggatggccc gcctgggcca
     1021 ccgcctcgcg cgccgcctgg cggctgcgct cgggcttgga tcgctcggcc atgccgggca
     1081 atggtgacgc atgaagccgt caggagggcc gcgggaactg cgtgcccacg aaccggtccc
     1141 acatgcggtc gctgagcagc ttgcgctggc ccatgatgat ccgcgccgac ggcgtgaccg
     1201 tgtaccgggg ccggggccgg cgggtggtca gcgccttctc gatcaccttc gccaccgcct
     1261 ccggcggccc gcccagcttg gccgtggggc cctcgtaggc accggcggtg gccttgccca
     1321 cggcgcggtt gaagtcggcg tagggcccgt cgccggccgt gccggcgtcg atcgaggcgg
     1381 tggccgcgct ggcgaagtcc gagacgatca ggcccggctc gatcaggacc acgtccacgc
     1441 cgaagccctt gacctcgaac cgcagcgcgt cgctcagcgc ctcgaccgag tacttggtgg
     1501 cgtggtagat cccgccgccg gggaaggtca gcttcccgcc catcgagccc acgttcacga
     1561 tccgcccgcg gcccgcctcg cgcatgccgg gcagcaccag ctggcacatc cggatcaggc
     1621 cgaagacgtt ggtctggaac tgggcccgga tgtcgtcgat gttcaccgac tccaccgcgc
     1681 ccgactggga gtagccggcg ttgttgacca gcgccccgac ggggccgtcg gcctcctcga
     1741 cggcggccac ggcggcccgc atcgactcct cgtcgttcac gtccagggcc agggtgcggc
     1801 agcccttggc ctcgaggtcg gccaccgcct cgggcttgcg cgcggtcgcg tacacggtgt
     1861 gcccggcggc ggccaggcgc tcggccgtgg cacggccgat gccggtcgag cagccggtga
     1921 tcaggacggg tcccttcacg gggtcagcac cacctttccg atgttgcggc gctcgccgat
     1981 gtagcggtgg gcctcaccgg cgcggtcgaa cgggaacgag gcggccacca ccggctccag
     2041 gaccccctgg tccagcaggt cctcgatcgc ggccatgaac ggctccagcg agccaagctc
     2101 gtcccagatg gtcagcatgt tcagcccgat cacggacttc gaggccgaca tctgcttgat
     2161 caggttgaag cgcggcatgg tcagcgcggt ccgcccggcg gcgatcaggt tgcgcttgtc
     2221 gccctccacc accccggccg cgccgaaggc gatcaggcgc ccgccggcgc ggagcatgtc
     2281 gtagctgcgg cggaagctct tgcccccgag ggcgtccatg accacgtcca tgggcggcag
     2341 ccccttctcc cagccgctcc gggtgtagtc caccgcgtgg tccacgccga agccccggat
     2401 ggcgtcgtgc ttgccgggcg aggcgctgcc ccagacctcg gcgccgcggt gcttggcgat
     2461 ctgcgtggcc gcgatgccca cgccgcccgc ggccgcgtgc aggaacacgc gctcccccgg
     2521 ctccagcgcg ccgaagcggc gcagcccgag gtaggcggtc aggtagttca ccggcaccgc
     2581 ggcgccctcc tcgaacgaga ggctctcggg cagcggcgcc gtgtcggtca cgttggccac
     2641 gacctgctcg gagtagccac cgaagcgcgt ccccgccatc acccgctcgc cgacggtgta
     2701 gcggtcgacc ccctcgccga gctcggccac gatgccggcg acctcgtagc ccaccaccgc
     2761 aggcaggtcg ggggcgtcgg ggtacaggcc cacgcgcgcc atcaggtcgg cgaagttcac
     2821 gcccgcggcg cccacctcga cgcgcacctg gccgggcccc ggccgggggt cggggcgctc
     2881 ctgcacctgc aggacctcgg gcgggcccgc cttggtgatg accaccgacc tcatgggccg
     2941 accctaccgg ccccggaacg cagcggggcc gcccgaaggc ggccccgtgc gctggtgata
     3001 ccgcgaccgg ccaagggtcg cgggggaccg ggggcgcgag cccccggacg gatacctatg
     3061 cggcggcggg cgtctccgcg cggcgcggca tcagcgtctc gcgggcgatg accaggcgct
     3121 ggatctggct ggtgccctcg tagagctgca tgatcttggc gtcacgcatc agcttctcga
     3181 ccgggtactc cttgatgaag ccgtacccgc cgtagacctg cacggcgtcg gtggtgacct
     3241 ccatcgcgga gtccgcggcg aagcgcttgg cgtgcgagga ctcgagcgtg ttgcgcttgc
     3301 cctggtccag cagcacggcc gagttccacg tggcgatgcg cgacaggtgc accttggtcg
     3361 ccatgtcggc gatcatgaac tggatcgcct ggtgcatcgc gatcggcacg ccgaactgca
     3421 cgcgctcctt ggagtactcg gtcgcgaact cgaaggccgc gcgcgagatg ccggtggcca
     3481 tggccgccac gccggggcgc gtgcggtcca gggtcatcat ggcgagcttg aagcccttgt
     3541 tctcctcgcc gagcatgttc tcggcgggga tctccacgtc gttgaaggag atcgtggcgg
     3601 tgttggaggc acgctgtccg agcttgtcct ccttcttgtc cacggtcacg cccggcaggt
     3661 cgcggtcgac gatgaacgcc gagatgccgc ggtggcccgc ctccttgtcc gtcttggcgt
     3721 agacggtgta gaagctggcg tacgagccgt tggtgatgaa gcacttggag ccgttgatga
     3781 cgtacttgtc acccttcttc acggccgtgg tcttcatgcc cgagacgtcg gagccggcgt
     3841 cgggctcgg
//
LOCUS       DASUPW010000131         2570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1553735, whole genome shotgun sequence.
ACCESSION   DASUPW010000131 DASUPW010000000
VERSION     DASUPW010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553735"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..1617
                     /locus_tag="VFY44_03000"
     CDS             220..1617
                     /locus_tag="VFY44_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909434.1"
                     /translation="MAPPLDGAPATRAGVRLTVMGDAGRVDLAVDPATASVALAREYA
                     EQLGLPDVPALCSLRGDDLAPHRSLAELGLRSGAVVLAVPSGPPSTHPSDPAAPSRPG
                     SEGPQALPARPAVLLLSVVLAAASAATATLPSYPGPARWFCVAIFLGCLVGVVARSGR
                     LDGADAESLAVTAPAFAAAAGWTAGYSPEPGGVQVGLLAAGLMAVVGAAVSRAAVSLD
                     RDERLRVWLVGGAALGAAALLLLLGGADAAVLLALAYGVGVAAARLLPYAAVDVPDDV
                     LLDLDRLAVTSWSARERPRGRRRAMVRPENVAEVVRRGQGVLLTGTLAIALSASIAGP
                     AALWATRGQGASAIGTLVMVAAGGCALALTARTVRSRVPRAALRLAAVVDLTVLGALL
                     LSRHAPGSPWPWLAGAWVLGGLALLSAVLLGRGWRSVWWARTADVAETLCVVVVLAAL
                     PVATGLFTVVRGLPG"
     gene            1857..2138
                     /locus_tag="VFY44_03005"
     CDS             1857..2138
                     /locus_tag="VFY44_03005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HEX5909435.1"
                     /translation="MTRGAQMVVSARGDLDQQLSALRGKLSGIGAQWRGAGSSAFQQV
                     MVRWDEDARKIISALNEFEANLRTSEQTYNANDESQSATFNKFAGRLGG"
     gene            2195..2485
                     /locus_tag="VFY44_03010"
     CDS             2195..2485
                     /locus_tag="VFY44_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HEX5909436.1"
                     /translation="MTGELKVNFGSLDAAAADIQTSANQIEARIDQLESELAPLRSDW
                     TGSASASYQQAKAKWDAAMADMKALLAEVGVAVTNSNSEYQATESQNAARWG"
BASE COUNT          314 a          954 c          949 g          353 t
ORIGIN      
        1 tgggcgggtc gatctcccgc ccacgtccta gcgtcgtgga cacgcagcgc ctccggccga
       61 gtcgagtgct ggggggtggg gaaacggcgg acgggcgtcg cgaacccctc gaaacgtgca
      121 gaatagtacg aggtggagcc acccccgcgg gggcgaagtg accacagcca ttcgtaccgg
      181 tgatcgtcgc gatcaccccg cactcggagg ggagagccca tggcgccacc gctcgacggc
      241 gcccccgcga cccgagccgg cgtgcggctc accgtgatgg gcgacgcggg ccgggtggac
      301 ctggccgtgg acccggccac cgcgagcgtc gcgctcgccc gcgagtacgc cgagcagctg
      361 ggcctgcccg acgtgcccgc gctgtgctcc ctgcgcgggg acgacctggc gccgcaccga
      421 tcgctcgccg agctcgggct gcgctcgggc gcggtggtgc tcgccgtccc ctccggtccg
      481 ccgtccacgc acccttccga cccggctgcc ccgtcccgac cagggtccga ggggccccag
      541 gccctccctg cccggcccgc cgtgctcctc ctgtccgtcg tgctcgccgc ggcctcggcc
      601 gcgaccgcga ccctcccctc ctacccgggc ccggcccggt ggttctgcgt ggcgatcttc
      661 ctgggctgcc tggtcggcgt ggtcgcccgc tcggggcgac tggacggcgc cgacgccgag
      721 tcgttggccg tgaccgctcc cgcgttcgcc gccgcggccg gatggaccgc gggctactcc
      781 ccggagccag gcggggtcca ggtgggcctg ctggcagcgg gtctgatggc ggtggtcgga
      841 gccgcggtga gccgcgccgc ggtctccctg gaccgagacg agcggctacg ggtgtggctg
      901 gtcggcggag ctgcgctcgg ggccgcggcc ctcctgctgc tgctcggtgg ggccgacgcc
      961 gccgtgctgc tcgccctggc ctacggcgtg ggagtggccg ccgcccggct gctgccgtac
     1021 gcagcggtgg acgtccccga cgacgtgctg ctcgacctcg accggctcgc cgtcacctcc
     1081 tggtcggctc gcgagcggcc ccgcgggcga cgccgcgcca tggtgcgccc ggagaacgtc
     1141 gccgaggtgg tgaggcgtgg ccagggggtg ctgctcaccg gcacgctcgc gatcgcgctg
     1201 agcgcctcga tcgccggccc tgcggccctc tgggcaaccc gcggccaggg cgcgtccgcg
     1261 atcggcacgc tggtcatggt ggctgccggc ggctgcgcgc tggcgctcac cgctcgcacc
     1321 gtgcgctccc gtgtgccgcg ggcggcgctg cggctggccg cggtggtcga cctgaccgtg
     1381 ctcggcgccc tgctcctgtc gcgccacgcg cccggctcac cgtggccgtg gctggccggc
     1441 gcgtgggtgc tgggaggtct ggcgctgctg tcggccgtgc tgctggggcg gggatggcgc
     1501 tcggtctggt gggcgcgcac ggccgacgtg gcggagacgc tctgcgtggt cgtggtgctg
     1561 gcggccctgc cggtggccac ggggctgttc accgtcgtcc gggggctgcc tgggtgagca
     1621 gcggaccggg cgggattacc cccttcgggg acgtgtgaga gcgtggccca cggggatagt
     1681 cccgaatagc atcaataggt acattaaacc gaaccgtcgg ccggggcacc cggccgaccg
     1741 aacggcgtgg ctggcgcacc actggatcca ctggactcca cgggaccgtc gcacgcaccg
     1801 tggcaccgaa aggggaccgg cccatggctg gtgaggtatc caagcaagat caggcattga
     1861 cccgtggcgc ccagatggtg gtgtccgcgc gcggtgacct ggaccagcag ctgagcgccc
     1921 tgcgcggcaa gctgtccggc atcggcgcgc agtggcgcgg cgccggctcc agcgccttcc
     1981 agcaggtgat ggtgcgctgg gacgaggacg cgcgcaagat catctccgcg ctcaacgagt
     2041 tcgaggccaa cctgcgcacc tccgagcaga cctacaacgc caacgacgag agtcagtcgg
     2101 ccaccttcaa caagttcgcc ggccggctcg gcggctgagc ccgctccgct gcacagccgt
     2161 caccacaggt caacgagacg agggatccac agacatgacc ggagagctca aggtcaactt
     2221 cggcagcctg gacgcggccg ccgccgacat ccagacctct gccaaccaga tcgaggcccg
     2281 catcgaccag ctcgagtccg agctggcccc gctgcgctcg gactggaccg gctcggcgtc
     2341 ggcgtcctac cagcaggcga aggccaagtg ggacgccgcc atggccgaca tgaaggccct
     2401 gctggccgag gtcggcgtcg ccgtcaccaa ctccaactcc gagtaccagg caaccgagag
     2461 ccagaacgca gcccgctggg gctgaggctc caccgcgtct cgagcgtggg tcccgtcgcc
     2521 cgcggcctgc tcggccgcgg cggcgggccc gcgctcgtct cgcccaccac
//
LOCUS       DASUPW010000132         5430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_308204, whole genome shotgun sequence.
ACCESSION   DASUPW010000132 DASUPW010000000
VERSION     DASUPW010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308204"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="VFY44_03015"
     CDS             <1..755
                     /locus_tag="VFY44_03015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX5909437.1"
                     /translation="GIAPYVGGRILRNHTLLTRELAEKAELAAHLGDAAERDAVNAER
                     VRVARELHDVLAHELSVMVIQAQGARRMIERDPEAAAEAADLIGRTGRDALTELRRLF
                     GSVRHGEGEDLSGPPGLGRLDELVGRARAAGLPVELRTEGEATTLAPGMDIAAYRIVQ
                     EALTNTYKHAGRAQAIVTVTHWPELVMLEIVDDGQGAPEVDASVFSGGHGLVGMRERV
                     EVYGGEVEAGPRQGGGFRVVARLPVERGKVAA"
     gene            752..1480
                     /locus_tag="VFY44_03020"
     CDS             752..1480
                     /locus_tag="VFY44_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5909438.1"
                     /translation="MSDVVGKQPARPDAQPGRTRVLLVDDQSLIRTGFRMILDAEEDI
                     EVVGESADGTQAIDSVNRFKPDVVLMDIRMPEMDGIEATRRIVAAAPEDQPVRVLMLT
                     TFDLDEYVYDALRAGASGFLLKDVPADQLVNGIRLVAAGDSLLAPSVTRRLIEEFSRT
                     PAADATPPPSFDDLTARELEVLKLIARGMSNAEIAAELIVSETTVKTHVTRVLLKLGV
                     RDRVQAVVAAYENGLVAPGSRPAA"
     gene            1483..2307
                     /locus_tag="VFY44_03025"
     CDS             1483..2307
                     /locus_tag="VFY44_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX5909439.1"
                     /translation="MAKRPFAQVDVFADRPQRGNPVAVVLDAGGVGDAALARFAAWTN
                     LSETTFVLPPTHEDADYRVRIFTPTVELPFAGHPTLGTCHAWLEAQGAGHEGDTIVQE
                     CDAGLVPIRRTGDGLAFAAPPLLRGGPVEESLVAELAEAMRVDRDDVIDAQWADNGPG
                     WVALMLGSAQAVLDVRPGYIEMDVGLVGMHPDGSPEVRAFFPKDGVLMEDPVTGSLNA
                     SLAQWLLGDGRLEAPFVARQGAAVGRDGRVHVSRDDHGTIWVGGQTVTCISGSVEL"
     gene            2633..3199
                     /locus_tag="VFY44_03030"
     CDS             2633..3199
                     /locus_tag="VFY44_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5909440.1"
                     /translation="MAYAGQTIENPVSGETIRFEQTAADTNGELLAFEVELTPDGHVP
                     GMHVHPEQEERFEVLAGTMKFRKGFKTVVAEAGDVVTVPAGTAHKFANAGDEPAVARV
                     EVRPALQMERLFETAVALAEEGKTTKKGMPKPLELALFTREFKREVQGAFPPAFVQHA
                     SMAPLRWIADRRGRGERYGRRGARVATA"
     gene            complement(3196..3693)
                     /locus_tag="VFY44_03035"
     CDS             complement(3196..3693)
                     /locus_tag="VFY44_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909441.1"
                     /translation="MEAMAAQSRHDREEAIAGAIYGTILTAATMSVVSDKRWDEPFLT
                     SAQVLVTLVVFWMAHAFAHGVAVRVTEEEPHPREMAHDLRATWPMVAAAFPQLAPMVL
                     VGLGVFTYDTGLWIAYALAVAILASYGVVIARRRRLGKAGTVRLVFVMGILGLILITL
                     KELIH"
     gene            3721..>5430
                     /gene="typA"
                     /locus_tag="VFY44_03040"
     CDS             3721..>5430
                     /gene="typA"
                     /locus_tag="VFY44_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX5909442.1"
                     /translation="MAQRTDIRNVAIVAHVDHGKTTLVDAMLWQSGAFREGQDVDDRV
                     MDSTDLEREKGITILAKNTAVDFDGVKLNIIDTPGHADFGGEVERGLTMVDGVLLLVD
                     ASEGPLPQTRFVLRKALESRLPVILVINKVDRPDARIAEVVDEVYELFLDLDADEAQI
                     EFPIVYTNAKAGWASLEEGVEGTDLKPLMKLIVDTVPAPEYDPEHPLQAQVTNLDASP
                     YVGRIALCRVRHGTIKQGQRVAWCKADGTTQSVTITNLYITESLDRVDADEAGPGEII
                     GVAGIPEITIGETLADAEDPRPLPVISVDEPSLSVIIGINTSPLAGQDGSKLTARQVK
                     ARLETELVGNVAIRVLDTERPDAWEVQGRGELQLAVLVEIMRREGYELTVGQPQVLIR
                     DIDGTAHEPFERLAIDTPEDYVGTITQMMALRKGRVEQHVNHGTGWVRMEYIVPARGL
                     IGFRTEFLTETRGTGIMHHVFEAWEPWAGSMRTRPSGSLVADRRGTTATFALFNLQER
                     GTMFVGPGEEVYEGMIVGENSRPDDLDVNAVKEKQLNNIRSSTAEVLVRLVPHRTLSL
                     DQAM"
BASE COUNT          830 a         1947 c         1944 g          709 t
ORIGIN      
        1 tcggcatcgc gccctacgtg ggcgggcgga tcctgcgcaa ccacaccctg ctgacgcgcg
       61 agctggccga gaaggccgag ctggcggcgc acctggggga cgccgcggaa cgcgacgccg
      121 tgaacgccga gcgcgtgcgc gtggcgcgcg agctgcacga cgtgctggcc cacgagctga
      181 gcgtgatggt catccaggcc cagggcgcgc gccggatgat cgagcgcgac cccgaggccg
      241 ccgccgaggc ggcggacctg atcggccgca ccgggcgcga cgcgctgacc gagctgcggc
      301 ggctgttcgg ctcggtccgc cacggcgagg gcgaggacct gtcgggcccg cccggcctgg
      361 gccggctgga cgagctggtg ggccgcgccc gcgccgccgg gctgccggtc gagctgcgca
      421 ccgagggcga ggccacgacg cttgcgccgg gcatggacat cgccgcgtac cgcatcgtcc
      481 aggaggcgct gaccaacacg tacaagcacg cgggccgcgc gcaggccatc gtcaccgtga
      541 cccactggcc ggagctggtc atgctcgaga tcgtggacga cggccagggc gcgcccgagg
      601 tggacgcctc cgtgttcagc ggcggccacg ggctcgtggg catgcgcgag cgcgtagagg
      661 tctacggtgg tgaggtcgag gcgggcccgc gtcagggcgg aggcttccgt gtggtggccc
      721 gcctgccggt cgaacgagga aaggtggccg catgagcgac gttgtgggca agcagccggc
      781 tcggccggat gcgcagccag gtcgtacccg ggtcctgctg gtggacgatc agtccctgat
      841 ccggaccggg ttccgcatga tcctggacgc cgaagaggac atcgaggtcg tcggcgagag
      901 cgcggacggc acccaggcca tcgacagcgt caaccggttc aagcccgacg tcgtgctgat
      961 ggacatccgc atgcccgaga tggatggcat cgaggccacc cgccggatcg tcgccgcggc
     1021 ccccgaggac cagccggtcc gggtcctgat gctgaccacc ttcgacctgg acgagtacgt
     1081 ctacgacgcc ctgcgcgcgg gcgccagcgg cttcctgctc aaggacgtgc ccgccgacca
     1141 gctggtcaac ggcatccggc tcgtggccgc cggcgactca ctgctcgccc cctcggtcac
     1201 gcgccggctg atcgaggagt tcagcaggac ccccgccgcc gacgccacgc cgccgccgtc
     1261 gttcgacgac ctgaccgcgc gcgagctgga ggtgctcaag ctgatcgcgc gcgggatgtc
     1321 caacgccgag atcgccgcgg agctgatcgt gtcggagacc acggtcaaga cccacgtgac
     1381 gcgcgtgctg ctcaagctcg gcgtgcgcga ccgcgtccag gccgtggtcg ccgcgtacga
     1441 gaacggcctc gtggcgccgg gctcgcggcc ggcggcgtag ccatggccaa gcgcccgttc
     1501 gcgcaggtcg acgtgttcgc cgaccggccc cagcggggca acccggtggc ggtcgtgctg
     1561 gacgccggcg gcgtgggcga cgccgcgctg gcccgcttcg ccgcctggac caacctgtcc
     1621 gagacgacct tcgtgctgcc gcccacccac gaggacgccg actaccgcgt ccggatcttc
     1681 acccccaccg tggagctgcc gttcgccggc caccccacgc tgggcacctg ccacgcctgg
     1741 ctggaggcac agggcgccgg gcacgagggc gacacgatcg tgcaggagtg cgacgccggc
     1801 ctggtgccca tccgccgcac cggcgacggc ctggccttcg ccgcgccgcc cctgctgcgc
     1861 ggcggcccgg tggaggagtc gctggtcgcc gagctggccg aggcgatgcg cgtcgaccgc
     1921 gacgacgtga tcgacgccca gtgggcggac aacggcccgg ggtgggtggc gctgatgctg
     1981 gggtcggccc aggcggtgct ggacgtgcgg ccgggctaca tcgagatgga cgtgggcctc
     2041 gtgggcatgc accccgacgg ctcgcccgag gtgcgcgcct tcttccccaa ggacggcgtg
     2101 ctcatggagg acccggtcac cggcagcctg aacgcctcgc tggcgcagtg gcttctgggc
     2161 gacggccgcc tggaggcgcc gttcgtggcg cgccagggcg ccgcggtggg gcgcgacggc
     2221 cgcgtccacg tgagccgcga cgaccacggc acgatctggg tcggcggcca gacggtcacg
     2281 tgcatctcgg ggtcggtcga gctgtagcgc cttccgtccg tcgacgccac cagtcgccga
     2341 acgcgaaacg gcggctgggt aaggcggccc cccaccgcgg atcatgcgtt tgggcggtcc
     2401 tggcgactca aacgcttgaa cggcttcgtc cgcgcgccct acccggcccg cgccctaccc
     2461 agccccaagg ccggtcggcc gaacgactgg tgcggcctct gcccactccc cctttggtcg
     2521 tacgccaaga ccatcccatc cgctgacacc ggatcgtcat cgagggcgat tccgggaacg
     2581 ccgcgcggat cgatgctggg gccatcaagc aatccggccc aggagatcca aaatggcgta
     2641 cgcaggacag accatcgaga accccgtcag cggcgagacc atccgattcg agcagaccgc
     2701 cgccgacacc aacggcgagc tgctggcctt cgaggtcgag ctgaccccgg acggccacgt
     2761 gcccgggatg cacgtgcacc ccgagcagga ggagcgcttc gaggtgctcg ccggcacgat
     2821 gaagttccgc aagggcttca agaccgtcgt ggccgaggcc ggcgacgtgg tcacggtccc
     2881 cgcgggcacc gcccacaagt tcgccaacgc cggcgacgag cccgccgtgg cgcgcgtcga
     2941 ggtccgtccc gcgctccaga tggagcggct gttcgagacc gcggtggcgc tggccgagga
     3001 gggcaagacc acgaagaagg gcatgcccaa gccgctggag ctggcgctgt tcacgcgcga
     3061 gttcaagcgc gaggtgcagg gcgccttccc gcccgccttc gtccagcacg cctcgatggc
     3121 gccgctgcgg tggatcgcgg accggcgcgg ccggggcgag cgctacgggc gccggggcgc
     3181 ccgggtggcc accgcctagt ggatgagctc cttcagggtg atcaggatca gcccgaggat
     3241 gcccatcacg aagaccagcc tgacggtgcc ggcctttccc agccggcgcc gtcgggcgat
     3301 caccacgccg taggaggcca ggatggccac ggccagggcg taggcgatcc acaggccggt
     3361 gtcgtaggtg aacacgccca ggcccaccag caccatcggc gccagctgcg ggaacgcggc
     3421 ggccaccatc ggccaggtcg cccggaggtc gtgggccatc tcgcgcgggt gcggctcctc
     3481 ttcggtgacg cgcaccgcca cgccgtgggc gaacgcgtgt gccatccaga acaccaccag
     3541 cgtgaccagc acctgggcgc tggtcaggaa cggctcgtcc cagcgcttgt cgctgaccac
     3601 cgacatggtc gcggcggtca ggatcgtgcc gtagatcgcg cccgcgatgg cctcttcgcg
     3661 gtcgtgccgg ctctgggcgg ccatggcctc catccgcggc acactaacct tgctcggttc
     3721 ttggcccagc gaacagatat ccggaacgtc gccatcgtcg cccacgtcga ccacggcaag
     3781 accaccctcg tggacgccat gctctggcag tccggcgcct tccgcgaggg ccaggacgtg
     3841 gacgaccgcg tcatggactc caccgacctg gagcgtgaga agggcatcac gatcctcgcg
     3901 aagaacaccg cggtcgactt cgacggcgtg aagctgaaca tcatcgacac gcccggccac
     3961 gccgacttcg gcggcgaggt ggagcgcggc ctgaccatgg tcgacggcgt cctgctgctg
     4021 gtcgacgcct ccgagggccc gctgccccag acgcgtttcg tgctgcgcaa ggcgctggag
     4081 tcccggctgc cggtgatcct ggtgatcaac aaggtcgatc gccccgacgc ccgcatcgcc
     4141 gaggtggtgg acgaggtcta cgagctcttc ctggacctgg acgccgatga ggcccagatc
     4201 gagttcccca tcgtctacac caacgccaag gcgggctggg ccagcctcga ggagggcgtc
     4261 gagggcaccg acctcaagcc gctgatgaag ctgatcgtgg acacggtgcc ggcgcccgag
     4321 tacgaccccg agcacccgct ccaggcccag gtgaccaacc tggacgcctc gccctacgtg
     4381 ggccggatcg cgctctgccg cgtgcgccac gggaccatca agcagggcca gcgcgtggcc
     4441 tggtgcaagg ccgacggcac cacccagtcg gtgacgatca cgaacctcta catcaccgag
     4501 tcgctggatc gggtggacgc ggacgaggcc ggtccgggcg agatcatcgg cgtggccggc
     4561 atccccgaga tcaccatcgg cgagaccctc gccgacgccg aggacccgcg cccgctgccc
     4621 gtgatcagcg tggacgagcc gtcgctgtcg gtgatcatcg gcatcaacac ctcgccgctg
     4681 gccgggcagg acggcagcaa gctgacggcg cgccaggtca aggcgcggct ggagaccgag
     4741 ctggtgggca acgtggccat ccgcgtgctg gacaccgagc gcccggacgc ctgggaggtc
     4801 cagggccgcg gcgagctgca gctggccgtg ctggtggaga tcatgcgccg cgagggctac
     4861 gagctgaccg tcggccagcc gcaggtgctg atccgcgaca tcgacggcac cgcccacgag
     4921 ccgttcgagc gcctggccat cgacacgccc gaggactacg tcggcacgat cacccagatg
     4981 atggccctgc gcaagggccg ggtggagcag cacgtcaacc acggcaccgg ctgggtgcgg
     5041 atggagtaca tcgtgccggc ccgcgggctg atcggcttcc gcaccgagtt cctgaccgag
     5101 acccgcggca cggggatcat gcaccacgtg ttcgaggcct gggagccctg ggccggctcg
     5161 atgcgcaccc gcccctcggg ctcgctcgtg gccgaccgcc gcggcaccac cgccaccttc
     5221 gccctgttca acctccagga gcgcggcacg atgttcgtgg gcccgggcga ggaggtctac
     5281 gagggcatga tcgtcggcga gaactcgcgt cccgacgacc tggacgtgaa cgcggtcaag
     5341 gagaagcagc tgaacaacat ccgctcctcc accgccgagg tgctggtgcg cctggtgccc
     5401 caccgcaccc tgagcctgga ccaggcgatg
//
LOCUS       DASUPW010000133         2555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1554238, whole genome shotgun sequence.
ACCESSION   DASUPW010000133 DASUPW010000000
VERSION     DASUPW010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554238"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="VFY44_03045"
     CDS             complement(<1..1194)
                     /locus_tag="VFY44_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX5909443.1"
                     /translation="MNRRWIAGTAVAVAALAGGTGQAHAGTLLASTATAGKAQPQNCI
                     KRPLPAGPGVVRRSAKAGGVGFVTATLEAARGDWDLAVFDARTRRLVAGSANFGSDER
                     AQGFSTGPQSLIVQACRRSGSARTARIAVTSTALKTPSAPVKLSLVRVLLPNRERKDE
                     LTRMGLDLTEHGGRNYVEVILHGAEDAKRLQEAKFAYTVEVADLYRQSKLDRAAEQRS
                     STRAVASALPSGRTGTYRRLPDYNQEMKTLAEQNPSLVKPLTLPFKTLTGMSVQGIEI
                     TEDVTERDGKPVFLQMGVHHAREWPSAENAFEFAYELVKGYKAGDARVRRLMKSSRVI
                     VVPIVNPEGFNTSREAGQAQGVAGGRPGNDTIETANLALFQYEYQRKNCRVNSFDPAV
                     PDPERG"
     gene            complement(1279..1725)
                     /locus_tag="VFY44_03050"
     CDS             complement(1279..1725)
                     /locus_tag="VFY44_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX5909444.1"
                     /translation="MRGDGPLLIEYADLECPYCAKTHFLIRDLPVRRVFRHFPVVSKH
                     PRARVLALAAEAAARQGTFWDFHDSLMADQGRLEDPHLWDRCRTLGLDLDRFEADRRD
                     AATADRVDRDFRAGIRAGVVTTPTLYVDGEAHPGIPGPELLDRLSG"
     gene            complement(1773..2228)
                     /locus_tag="VFY44_03055"
     CDS             complement(1773..2228)
                     /locus_tag="VFY44_03055"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX5909445.1"
                     /translation="MIEPGSRAPDFTLPDQDGQDVSLSDLEGQTSVLVFYPMDFSPVC
                     TDQLNVYQEVLPELEAKGVKLYGVSVDSSFAHKAFQEHLGVSIPLLADFHPKGQVARD
                     YGVFIDERGHNQRALVMIGPDLKVTWSHQAPTPLEIPGANLIFDALEQV"
     gene            complement(2225..2494)
                     /gene="rpsO"
                     /locus_tag="VFY44_03060"
     CDS             complement(2225..2494)
                     /gene="rpsO"
                     /locus_tag="VFY44_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX5909446.1"
                     /translation="MSLTVEQKQEIVTKHGRGDNDTGSAEVQVALLSARIEEHTEHLR
                     EHRKDHHSRRGLLMLVGKRRRLLNYLQRNDLDRYRALIKELGLRR"
BASE COUNT          341 a          868 c          943 g          403 t
ORIGIN      
        1 gccccgctcg gggtccggga cggccgggtc gaagctgttc acgcggcagt tcttgcgctg
       61 gtactcgtac tggaacaggg ccaggttggc cgtctcgatg gtgtcgttgc ccggccgccc
      121 gccggccacg ccctgcgcct ggccggcctc gcgggaggtg ttgaagccct cggggttgac
      181 gatcgggacg acgatcacgc ggctgctctt catcagccgc cgcacgcgcg cgtcgccggc
      241 cttgtagccc ttgaccagct cgtaggcgaa ctcgaacgcg ttctcggccg acggccactc
      301 acgggcgtgg tgcacgccca tctggaggaa caccggcttg ccgtcgcgct cggtcacgtc
      361 ctcggtgatc tcgatgccct gcacgctcat gccggtcagc gtcttgaacg gcagcgtgag
      421 cggcttgacc agcgacgggt tctgctcggc cagggtcttc atctcctggt tgtagtccgg
      481 caggcgccgg taggtcccgg tgcgcccgct gggcagcgcg gaggccaccg cacgggtgga
      541 gctgcgctgc tcggccgcgc ggtcgagctt cgactggcgg tagaggtcgg cgacctcgac
      601 ggtgtaggcg aacttggcct cctggaggcg cttggcgtcc tcggccccgt gcaggatcac
      661 ctcgacgtag ttgcgcccgc cgtgctcggt caggtccagg cccatcctgg tcagctcgtc
      721 cttgcgctcg cggttgggca gcaggacgcg caccagcgac agcttgaccg gggcgctggg
      781 ggtcttgagc gcggtcgagg tcaccgcgat gcgggcggtc cgtgcggacc cggagcggcg
      841 gcaggcctgg acgatcaggc tctgggggcc ggtgctgaag ccctgggcgc gctcgtcgga
      901 gccgaagttg gccgagccgg ccacgagccg gcgggtcctg gcgtcgaaga cggccaggtc
      961 ccagtcgcca cgcgccgcct cgagggtggc ggtcacgaag ccgacgccgc cggccttggc
     1021 gctcctgcgc accacgccgg gaccggcggg cagcgggcgc ttgatgcagt tctggggctg
     1081 ggcctttccg gccgtggccg tggaggccag cagcgtgccc gcatgtgcct gcccggttcc
     1141 accggccagt gcggccaccg cgacggcggt gccggcgatc caacgacgat tcataccctg
     1201 cctctcaaga gtgcgtaccc gtgccggcag ggtttttaca gcacggagga cccccacgcg
     1261 ccacccgcag ggcccaactc accccgacag acggtccagg agctccggcc cgggaatgcc
     1321 cgggtgggcc tccccgtcga cgtacagcgt cggggtggtc accacgcctg cgcggatgcc
     1381 cgcccggaag tcccggtcca cccggtcggc ggtcgcggcg tcccggcgat cggcctcgaa
     1441 ccggtccagg tccaggccca gcgtgcggca ccggtcccac aggtgcgggt cctccagccg
     1501 gccctggtcg gccatcagcg agtcgtggaa gtcccagaag gtcccctggc gcgctgcggc
     1561 ctcggcggcc agcgccagga cgcgggcgcg cgggtgcttg gagaccaccg ggaagtggcg
     1621 gaacacgcgc cggacgggca ggtcgcggat caggaagtgg gtcttcgcgc agtacgggca
     1681 ctccaggtcc gcgtactcga tcagcagcgg gccgtccccg cgcacgtggt cgtcggcacc
     1741 gacgtccggg accggcgagg agccgaggtc gctcagacct gctcgagcgc gtcgaagatc
     1801 aggttggcgc ccgggatctc cagcggggtg ggtgcctggt gggaccaggt gaccttcagg
     1861 tcggggccga tcatgaccag cgcgcgctgg ttatgcccgc gctcgtcgat gaagaccccg
     1921 tagtcgcggg ccacctgccc cttgggatgg aagtcggcca gcaacgggat cgacacgccc
     1981 aggtgctcct ggaaggcctt gtgcgcgaac gaggagtcca ccgacacgcc gtagagcttg
     2041 acgcccttgg cctcgagctc gggcaggacc tcctggtaga cgttcagctg gtccgtgcag
     2101 accgggctga agtccatcgg gtagaagacc aggacgctgg tctgcccctc gaggtccgag
     2161 agggagacgt cctgtccgtc ctggtccggc agcgtgaagt cgggcgcgcg gctgcccggc
     2221 tcgatcatcg gcgcagcccg agttccttga tcagggcgcg gtagcggtcg agatcgttgc
     2281 gctgcaggta gttcaggagc cggcggcgct tgccgaccag catcagcagc ccgcggcggg
     2341 agtgatggtc cttgcggtgc tcgcgcaggt gctcggtgtg ctcctcgatg cgagccgaca
     2401 ggagggccac ctggacctcg gccgagccgg tgtcgttgtc accgcggccg tgcttggtga
     2461 cgatctcctg cttctgttcg acggtaaggc tcatgtgcgg cgcagaaagg tagcagggcc
     2521 taggtcgcca gcaggtcccg cgtgcgctgc acgtc
//
LOCUS       DASUPW010000134         2332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_30447, whole genome shotgun sequence.
ACCESSION   DASUPW010000134 DASUPW010000000
VERSION     DASUPW010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30447"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VFY44_03065"
     CDS             complement(<1..660)
                     /locus_tag="VFY44_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909447.1"
                     /translation="MSNGEQGGFGEAQLRRERLLDLEERIRPYRLAAFGVLGLSLLAS
                     GPWEGWWWLAPLAGALLAFSVGDRLRARSARPERWVAASWAVSPLMIAVSVALTGAAD
                     SPALGWFALPLVTLASRFEWRGTLVGIGYCVALLLASTVPFDPAAVVDDPVPLIHTFA
                     LMLAVTLLGGAIVQSDRDHRREAVLDPLTGLFNRAALAQRFADQRHDPEAVEDRRVGI
                     LI"
     gene            complement(709..>2332)
                     /locus_tag="VFY44_03070"
     CDS             complement(709..>2332)
                     /locus_tag="VFY44_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX5909448.1"
                     /translation="LFAPRGTTVEVARGGGSEGDLVLVGGGKKGARVVRRLGRPDVAR
                     DVVEALMLDRGLRRTFPRRVEEEADALEPAAGDERVDLRDLATFTIDPDDARDFDDAI
                     SAKRNDDGTIRVWVHIADVSAHVTAGGAIEAEAERRGTSVYVPGAVEPMLPEALSNGA
                     CSLRPGEDKLAVTVEMDMAGTDVRRVRFLRTLIRSDRRLTYGQVDEVFAGGARAEDPW
                     GGPLAAAREVATALAAKRRDAGALEVDSKEPSFAFDDAGHVTGVFHEAQTESHELIEH
                     LMILANEQVAGYLADRKLPTLYRVHEKPDPLKVASMVEQLASLGVPTPPVPEPMSPQQ
                     AADVAAEASRLAAAEARRSGRGATAFGSLVLRSLKQAYYSPRNLGHAGLASTRYCHFT
                     SPIRRYPDLVAHRALLAGLGLDPRAPRAADMDEAGIEASAREREAMKIERSADDVCLA
                     FLLERSIDPDDPVYEGEVVGLVGKGAFVRFGEEGFEGFLGVRGLDGWYELNELGTALD
                     SDRRSIRMGDPVRVAVERVEAPRGRVELQLVP"
BASE COUNT          313 a          895 c          845 g          279 t
ORIGIN      
        1 gatcaggatc ccgacccggc ggtcttcgac ggcctcgggg tcgtgacgct ggtcggcgaa
       61 gcgctgcgcc agcgcggccc ggttgaacag gcccgtcaga gggtccagca cggcctcgcg
      121 ccggtggtcg cggtcggatt ggacgatggc gccgcccagg agcgtcacgg ccagcatcaa
      181 cgcgaaggtg tggatcagcg gcaccgggtc gtcgacgacc gcggccggat cgaagggcac
      241 cgtcgaagcc aacaggaggg ccacgcagta gccgatcccc accagcgtgc cacgccactc
      301 gaagcgcgag gccagcgtga ccagcggcag cgcgaaccag cccagggcgg gcgagtcggc
      361 cgcgccggtc agcgccacgc tcacggcgat catcagcggc gagacggccc agctggcggc
      421 gacccagcgc tcgggccgcg cgctgcgggc gcgcaggcgg tcgccgaccg agaaggccag
      481 cagcgcgccg gccaggggag ccagccacca ccagccctcc cagggacccg acgccagcag
      541 cgacaggccc aggacgccga acgctgcgag ccggtagggg cggatgcgct cctcgaggtc
      601 gagcaggcgc tcacggcgca gttgagcctc accgaaccct ccctgctccc cgttggacat
      661 acggtcctgt cggcacctgg ccgcggcact tgagcgtttc ggcgcccgtc agggcacgag
      721 ctggagctcg acgcggccgc gcggggcctc gacccgctcc acggccaccc gcacggggtc
      781 gcccatgcgg atcgagcggc gatccgagtc cagcgccgtg cccagctcgt tgagctcgta
      841 ccagccgtcc aggccgcgaa cgcccaggaa gccctcgaag ccctcctcgc cgaagcgcac
      901 gaaggcgccc ttgcccacca ggcccacgac ctcgccctcg tagaccgggt cgtcggggtc
      961 gatggagcgc tcgagcagga aggccaggca cacgtcgtcg gccgagcgct cgatcttcat
     1021 ggcctcgcgc tcgcgggcgc tggcctcgat ccccgcctcg tccatgtcgg cggcgcgcgg
     1081 ggcgcggggg tccagcccca gcccggccag cagggcgcgg tgggccacca ggtcggggta
     1141 gcgcctgatg ggcgaggtga aatggcagta gcgcgtgctg gccagcccgg cgtgaccgag
     1201 gttgcgcggc gagtagtagg cctgcttgag cgagcgcagg accagcgagc cgaacgcggt
     1261 ggcgccgcgg ccgctccggc gcgcctcggc cgccgccagg cgcgacgcct cggcggccac
     1321 gtccgcggcc tgctgcgggc tcatcggctc gggcaccggc ggggtcggca cgcccagcga
     1381 ggccagctgc tcgaccatgc tcgccacctt cagcggatcg ggcttctcgt gcacccggta
     1441 gagcgtgggc agcttgcggt cggccaggta gccggccacc tgctcgttgg ccaggatcat
     1501 caggtgctcg atcagctcgt gcgactcggt ctgggcctcg tggaagaccc cggtcacgtg
     1561 gccggcgtcg tcgaaggcga acgacggctc cttgctgtcg acctccagcg cgcccgcgtc
     1621 gcggcgcttc gcggccaggg ccgtggccac ctcgcgcgcg gcggccagcg ggccgcccca
     1681 ggggtcctcg gcgcgcgcgc cgccggcgaa gacctcgtcc acctggccgt aggtcagccg
     1741 ccggtcgctg cggatgagcg tgcgcaggaa ccgcacccgg cgcacgtcgg tgccggccat
     1801 gtccatctcc accgtgaccg ccagcttgtc ctcgccgggg cggagcgagc aggcgccgtt
     1861 ggacagcgcc tcgggcagca tcggctccac cgcgccgggc acgtacacgc tggtcccgcg
     1921 ccgctcggcc tcggcctcga tggcgccccc ggccgtcacg tgcgcgctca cgtcggcgat
     1981 gtgcacccac acccggatcg tgccgtcgtc gttgcgcttg gccgagatgg cgtcgtcgaa
     2041 gtcgcgggcg tcgtcgggat cgatcgtgaa cgtggccagg tcgcgcaggt ccacgcgctc
     2101 gtcgcccgcc gccggctcca gcgcgtcggc ctcctcctcc acgcggcgcg ggaacgtccg
     2161 ccggaggccg cggtccagca ttagcgcctc gaccacgtcc cgggccacgt cgggccggcc
     2221 caggcggcgg accacccgcg cgcccttctt gccgccgccg accagcacca gatcgccctc
     2281 ggaaccgccc ccgcgcgcca cctcgaccgt ggtcccgcgc ggtgcgaaca gc
//
LOCUS       DASUPW010000135         3407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1416187, whole genome shotgun sequence.
ACCESSION   DASUPW010000135 DASUPW010000000
VERSION     DASUPW010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416187"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VFY44_03075"
     CDS             complement(<1..202)
                     /locus_tag="VFY44_03075"
                     /inference="COORDINATES: protein motif:HMM:NF016244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HEX5909449.1"
                     /translation="MSETPSSAGDGGGDRIDWIAAEKSPEFQELTKTRNRFVVPATIF
                     FLVWYLGFILLSGQAKDFMGREF"
     gene            complement(277..543)
                     /gene="atpE"
                     /locus_tag="VFY44_03080"
     CDS             complement(277..543)
                     /gene="atpE"
                     /locus_tag="VFY44_03080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEX5909450.1"
                     /translation="MEELATFVIAQADSKGLQSIALGVGAGLGTIGPGIGVGYIFGKV
                     IESVTRQPEMREEITSIQWLGFALTEAIVFYAFIFGLIAFFLGG"
     gene            complement(628..834)
                     /locus_tag="VFY44_03085"
     CDS             complement(628..834)
                     /locus_tag="VFY44_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909451.1"
                     /translation="MTRRQRTLRFGAMALVIALGLAALGLASGIVLETLGVFLLLVGF
                     LGLLMLLYLEVGLSEDRDRGRRGP"
     gene            complement(831..1565)
                     /locus_tag="VFY44_03090"
     CDS             complement(831..1565)
                     /locus_tag="VFY44_03090"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX5909452.1"
                     /translation="MSGDGPRTATGAGEARAQRSLAARFTRAGMRVSTQPVRVPGKGR
                     SRNVIGVRPGRGRCLHVVMAHADTVAGSPGANDNASGLGVLAELARRTRAGGLPCELW
                     LVGTGAEERNFTGARDHLGALALARLVRHNRGSARLRWALSLDEVGRRRSLVLRSPAP
                     AQRGGVERAMAAAARRERVGLRWQRDATGGLSDHREFHLLGLRAMKLGSSASCYHQPC
                     DTGGALDRVALGRHVRVVSRTVASGR"
     gene            complement(1841..3130)
                     /locus_tag="VFY44_03095"
     CDS             complement(1841..3130)
                     /locus_tag="VFY44_03095"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX5909453.1"
                     /translation="MPTEMENPLHKLTEEQIEAIGKEFDDLHDEVFDSLGERDAAYIR
                     GMIALHRRLVLIARVELLAAKYKPMWVLGTATLGAAKILENMEIGHNVMHGQWDWMND
                     PVINSTNWDWDTASTKEAWKHSHNYIHHTYTNVLGLDKDLGYEVMRIDPEQKWHPRYL
                     GQPILNLFLMAFFEWGVAVHDMDIEAIRAGEKKWADVKDDLKGMAGKAKAQVIKDYIA
                     FPALSAGATTAVELGVRKLLESRRPRSQKLADKLPGKLRRKPAPPTPAEIALETFRKT
                     FWADFTANIIRNVWSNAIIFCGHFPDQAYVFKKEDYENETRGARYVRQLLGAVNIDGG
                     PMFHVASGNLSYQVEHHLYPDMPSTRYGEIAPKVREICERYELPYNTGPFLQQWGMVQ
                     RTILRLAFPGGKPRPKPGPYQGEKVRDKGETGDRVAA"
     gene            complement(3149..>3407)
                     /locus_tag="VFY44_03100"
     CDS             complement(3149..>3407)
                     /locus_tag="VFY44_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEX5909454.1"
                     /translation="EGGTIKFLKSDTEAESDGSQPILVAGEEAGLELAYGCREGICHT
                     CVGTLKSGQIRDLRTGKVSGDEGETIRTCINAPEGAIEIDL"
BASE COUNT          482 a         1231 c         1145 g          549 t
ORIGIN      
        1 tgaactcccg gcccatgaag tccttggcct ggccggacag caggatgaag ccgaggtacc
       61 agaccaggaa gaagatcgtg gccggcacca cgaagcggtt gcgtgtcttg gtgagctcct
      121 ggaactcggg agacttctcg gccgcgatcc agtcgatccg gtcgccgccc ccatcccccg
      181 cactactggg cgtttcgctc atctgcgtct cctccccgga gattgacgat tgccacggcc
      241 cgtacccgcc cgggccgtgg cgcaatcgcc ttccggtcag ccgcccagga agaacgcgat
      301 caggccgaag atgaacgcgt agaacacgat tgcctcggtc agcgcgaagc ccagccactg
      361 gatgctggtg atctcctcac gcatctccgg ctggcgggtg accgactcga tcaccttgcc
      421 gaagatgtag cccacgccga tccccgggcc gatggtgccc agcccggccc cgactcccag
      481 cgcgatggac tgcaggccct tcgagtccgc ctgggcgatc acgaatgtcg cgagctcttc
      541 catcgggtgc tcctctctgt ggggtgcacc gatggtcccg ctacacgggc ccggaatcca
      601 tgacccggcg ggtaagcgtg aggcatctca cggtccgcgc cgaccgcggt cgcggtcctc
      661 gctcaggccg acctcgaggt acagcagcat cagtaggccc aggaagccca caagcagcag
      721 gaacacgcca agcgtctcga gcacgatccc ggaggccagc ccgagcgcgg ccaggccgag
      781 cgcgatcacc agggccatgg cgccgaagcg cagggttcgc tgccgccggg tcatcgtccc
      841 gaggccactg tccgggacac gacccggaca tgcctgccca gcgccacccg gtccagggcg
      901 ccgccggtgt cgcacggctg gtggtagcac gacgcgctcg agccgagctt catcgcccgc
      961 agccccagca ggtggaactc acggtggtcg gacaggccgc cggtggcgtc gcgctgccag
     1021 cgcaggccga cgcgctcacg gcgggcggcg gccgccatcg cccgctccac cccgccccgc
     1081 tgggcgggcg ccggggaccg cagcaccagc gagcgccggc gccccacctc gtccagcgac
     1141 agcgcccagc gcagccgggc cgagccgcgg ttgtgccgca ccaggcgcgc cagcgccagc
     1201 gcgccgaggt ggtcgcgggc gccggtgaag ttgcgctcct ccgcgcccgt ccccaccagc
     1261 cacagctcgc acggcaggcc gccggcgcgg gtgcgccggg ccagctccgc caggacgccg
     1321 aggcccgagg cgttgtcgtt cgcgcccggc gaaccggcca cggtgtcggc gtgggccatg
     1381 accacgtgca ggcagcgccc gcgcccgggc cggacgccga tcacgttgcg cgagcggccc
     1441 ttgcccggga cgcggacggg ctgggtggac acgcgcatgc cggcgcgcgt gaagcgcgcg
     1501 gccagcgacc gctgggccct ggcctcaccg gcgccggtgg ccgtgcgcgg gccgtcaccg
     1561 gacaccgcgc gcgcgaaccc cagcgccgtg tctccggtcg cagcgctcgc cgccccgggg
     1621 gcggcaagcg tcgcgaccag ggcccatacc gctgcccggt aagaagagcg cccccgcgca
     1681 ggctggtgcg cgggggcgtc cagggatcgc atctctcctc tcaatcccag ccggcggagg
     1741 ggacgctcgg tccccaacgt cgtcggccta tgtgcgaaat gtaacgcaat ccggcccgcg
     1801 gtgtcgtgaa acgtttcacg accgcccagg cgggctgctc tcaggccgcg acgcggtccc
     1861 ccgtctcgcc cttgtcgcgc accttctcgc cctggtacgg gcccggcttc ggccgcggct
     1921 tgccgcccgg gaacgccagc cgcaggatgg tgcgctgcac catgccccac tgctgcagga
     1981 atgggccggt gttgtagggc agctcgtagc gctcgcagat ctcgcgcacc ttgggcgcga
     2041 tctcgccgta gcgcgtgctc ggcatgtccg ggtacaggtg gtgctcgacc tggtagctca
     2101 ggttcccgga ggccacgtgg aacatgggac cgccgtcgat gttcacggct ccgagaagct
     2161 ggcgcacgta ccgggcgccg cgggtctcgt tctcgtagtc ctccttcttg aacacgtacg
     2221 cctggtcggg gaagtgcccg cagaagatga tcgcgttgga ccacacgttg cggatgatgt
     2281 tggcggtgaa gtcggcccag aacgtcttgc ggaacgtctc gagggcgatc tcggcggggg
     2341 tcggcggcgc cggcttgcgg cgcagcttgc ccggcagctt gtcggccagc ttctgcgagc
     2401 gcgggcggcg cgactccagg agcttgcgca ccccgagctc gaccgcggtc gtcgctccgg
     2461 cgctgagcgc cgggaacgcg atgtagtcct tgatcacctg ggccttggcc ttgccggcca
     2521 tgcccttcag gtcatccttg acgtcggccc acttcttctc gccggcgcgg atggcctcga
     2581 tgtccatgtc gtgcaccgcg acgccccact cgaagaaggc catcaggaac aggttcagga
     2641 tcggctggcc caggtacctg gggtgccact tctgctcggg atcgatgcgc atgacctcgt
     2701 agcccaggtc cttgtccagc ccgaggacgt tcgtgtaggt gtggtggatg tagttgtggg
     2761 agtgcttcca cgcctccttg gtggacgcgg tgtcccagtc ccagttggtg gagttgatga
     2821 ccgggtcgtt catccagtcc cactggccgt gcatcacgtt gtggccgatc tccatgttct
     2881 ccaggatctt ggccgcgccc agcgtggccg tgcccagcac ccacatcggc ttgtacttcg
     2941 cggccaggag ctccacgcgc gcgatcagca ccagccggcg gtgcagcgcg atcatcccgc
     3001 ggatgtacgc cgcgtcgcgc tcgccgagcg agtcgaacac ctcgtcgtgc agatcgtcga
     3061 actccttgcc gatggcctcg atctgctcct cggtcagctt gtgcagcggg ttttccattt
     3121 cagtgggcat tcgttcccct cccgttcatc aaaggtcgat ctcgatcgcg ccttcgggcg
     3181 cgttgatgca agtgcggatg gtctccccct cgtccccgga caccttcccg gttctcagat
     3241 ctcgtatctg acccgatttc agggtcccca cgcaggtgtg gcagatgccc tcgcgacagc
     3301 cgtaggccag ctcgaggccg gcctcctcgc cggcgacgag gatcggctgc gagccgtcgc
     3361 tctccgcctc ggtgtcgctc ttgaggaact tgatcgtgcc gccctcg
//
LOCUS       DASUPW010000136         3020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_862930, whole genome shotgun sequence.
ACCESSION   DASUPW010000136 DASUPW010000000
VERSION     DASUPW010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862930"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1689)
                     /locus_tag="VFY44_03105"
     CDS             complement(<1..1689)
                     /locus_tag="VFY44_03105"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX5909455.1"
                     /translation="MSAERLVPDEREVLMQVASAAATASGLDELLERTSEAAQAAMGA
                     ASLSISKWERERSVMRTLINVGELGPGEERWPEDETYGLEEHASVDRLLRTAQPYFNA
                     VDDPGADPNAVALLQSLRKESDIGVPIVVEGEVWGEVWATTAPGSPRFTGRDVRFLEA
                     IAGQLGGVVARGEMFSRVSRLAYEDELTGLSNRRALDEQLAAALDTWRETGQSLTLLA
                     CDVDELKTINDERGHHAGDRALRRVGQALIKAAASVPGACVARMSGDEFAVVMVGADL
                     AEAGRVAATALRILREERDTAVSVSCGLAAAGPGTESPESLMRAADAAQYAAKRRGGA
                     QVCSAGRDDFHELISAGEASTQRRRGRRRSQAERTEEACGRVLDLLDGSLAHRATLDR
                     MEAVASTLAQVVNAAGWTVSFALHGDETIRSLAAADGRDSLLRGMRVGLEDEVYALSD
                     YPATQGLVAQGSGTFHVDRYDRSADRAERLLLDEIGYSAVLAAAATDPEGCFLLEMYA
                     DGDTRDLTGLTVSLSLLVRAAAANSASAMTVSERLRRRTRHITVTSAVGRRLAGA"
     gene            complement(1726..2520)
                     /locus_tag="VFY44_03110"
     CDS             complement(1726..2520)
                     /locus_tag="VFY44_03110"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX5909456.1"
                     /translation="MGDLTRRILFAIPLAVAAIFIVTGGKEVFAAGLAILGVLALSEL
                     YGLMRRARPIDLAGFMTVIAMIVLATYEERQSVLIALVAAFPLVFLLGLVRERLDNLA
                     WGVSATVFGICWIGLPLAHAVFLRELPHGQTLLVDVLIATFIGDTAAYLGGRAWGRTP
                     LAPDISPNKTLEGLAAGVLGGTIAFWAAGTYSDWLTGTDALAIGFLVAITAPLGDLFE
                     SALKRDLEVKDTSTLLGPHGGVLDRLDAVFFTVVVGFYAALAFGYG"
     gene            complement(2524..>3020)
                     /gene="uppS"
                     /locus_tag="VFY44_03115"
     CDS             complement(2524..>3020)
                     /gene="uppS"
                     /locus_tag="VFY44_03115"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX5909457.1"
                     /translation="TENWNRSENEVAGLMAMFGQRIDVETPELDEEGVRMRFIGRREG
                     VSEELQEKMDWAEQTTADNTRITLFVAFNYGGRAEIVDAARTFTDGDESDFARHLYAP
                     DMHDPDLLIRTSGEQRISNYLLWQCAYSEFVFRDELWPDFTTEAFEESLAEYARRGRR
                     FGGR"
BASE COUNT          419 a         1138 c         1064 g          399 t
ORIGIN      
        1 cgcgcccgcc agcctgcgcc cgacggcgct ggtcacggtg atgtggcggg tccggcggcg
       61 cagtcgctcg gacacggtca tggcgctggc ggagttggcg gccgcggcgc ggaccagcag
      121 cgacagcgac acggtcaggc cggtcaggtc gcgcgtgtcg ccgtccgcgt acatctccag
      181 caggaagcag ccctcggggt cggtcgcggc cgcggccagc acggccgagt agccgatctc
      241 gtccagcagc aggcgctcgg cgcggtccgc cgaccggtcg tagcggtcca cgtggaacgt
      301 gccgctgccc tgggccacca ggccctgggt ggccgggtag tccgacagcg cgtagacctc
      361 gtcctccagg ccgacgcgca tgccgcgcag cagcgagtcg cgcccgtccg ccgccgccag
      421 cgagcggatc gtctcgtcgc cgtgcagcgc gaaggagacg gtccaccccg cggcgttgac
      481 cacctgcgcg agagtgctgg ccacggcctc catgcggtcc agcgtggccc ggtgggccag
      541 cgagccgtcc aggaggtcca gcacccggcc acaggcctcc tcggtccgct cggcctggct
      601 gcgccggcgc ccgcggcggc gctgggtcga ggcctcgccg gcgctgatca gctcgtggaa
      661 gtcgtccctg ccggccgagc acacctgcgc gcccccgcgc cgcttggccg cgtactgggc
      721 ggcgtccgcg gcccgcatca gcgactcggg gctctcggtc cccgggcccg cggcggccag
      781 gccgcaggac accgaaacgg cggtgtcgcg ctcctcgcgc aggatgcgca gcgcggtcgc
      841 ggccacccgg cccgcctcgg ccaggtcggc ccccaccatg accacggcga actcgtcgcc
      901 cgacatgcgc gccacgcacg cgccgggcac cgacgccgcg gccttgatca gcgcctggcc
      961 cacgcgccgc agcgcgcggt cgccggcgtg gtgaccgcgc tcgtcgttga tggtcttgag
     1021 ctcgtcgacg tcgcaggcca gcagcgtcag ggactgcccg gtctcgcgcc aggtgtccag
     1081 cgccgccgcc agctgctcgt ccagggcacg gcggttggac aggccggtca gctcgtcctc
     1141 gtaggccagg cgcgacacgc gcgagaacat ctcgccgcgg gccacgacgc ctccgagctg
     1201 gccggcgatg gcctccagga agcgcacgtc gcggccggtg aagcgcggcg agcccggcgc
     1261 ggtggtggcc cagacctcgc cccacacctc gccctcgacc acgatcggca cgccgatgtc
     1321 ggactccttg cgcaggctct gcagcagggc gaccgcgttc gggtcggccc cggggtcgtc
     1381 gacggcgttg aagtagggct gcgccgtgcg cagcagccgg tcgacgctgg cgtgctcctc
     1441 caggccgtag gtctcgtcct cgggccagcg ctcctccccc ggccccagct cgccgacgtt
     1501 gatcagcgtg cgcatgaccg agcgctcgcg ctcccacttg ctgatggaca gcgatgcggc
     1561 ccccatcgcg gcctgcgcgg cctcggacgt gcgctccagc agctcgtcca gcccgctggc
     1621 ggtcgcggcc gccgatgcga cctgcatcag gacctctcgc tcgtcgggga ccagccgctc
     1681 tgcggacatc acggttgtat cgggcgcgga gcccgatctc ttgagctacc cgtagccgaa
     1741 ggcgagcgcg gcgtagaagc ccaccacgac cgtgaagaac acggcgtcca ggcggtccag
     1801 cacgccgccg tgcggcccca gcagcgtgct ggtgtccttg acctccaggt cgcgcttgag
     1861 cgcggactcg aacaggtccc ccagcggcgc ggtgatggcc accaggaagc cgatggccag
     1921 ggcgtcggtg ccggtcagcc agtcggagta cgtccccgcc gcccagaacg cgatcgtgcc
     1981 gcccagcacg cccgccgcca ggccctcgag ggtcttgttc ggcgagatgt cgggcgccag
     2041 cggggtgcgc ccccaggcgc ggccacccag gtaggcggcg gtgtcgccga tgaaggtcgc
     2101 gatcagcacg tcgaccagca gcgtctggcc gtgcggcagc tcgcgcagga acaccgcgtg
     2161 ggccagcggc aggccgatcc agcagatgcc gaagaccgtg gccgacacgc cccaggccag
     2221 gttgtccagg cgctcgcgga ccaggcccag caggaacacc agcgggaagg cggccacgag
     2281 cgcgatcagg accgattggc gctcctcgta ggtggccagc acgatcatcg cgatcaccgt
     2341 catgaagccg gccaggtcga tcgggcgggc gcgccgcatc aggccgtaca gctcgctcag
     2401 cgccagcacg cccaggatgg ccaggccggc ggcgaacacc tccttgccgc cggtcacgat
     2461 gaagatggcc gcaacggcca gcgggatcgc gaacaggatc cgccgggtca ggtcgcccac
     2521 gggctatctc cccccgaaac ggcgccctcg gcgggcgtac tcggccagcg actcctcgaa
     2581 cgcctccgtc gtgaagtccg gccacagctc gtcgcggaac acgaactccg agtaggcgca
     2641 ctgccagagc aggtagttgg agatgcgctg ctcgccgctg gtgcggatga gcagatcggg
     2701 atcgtgcatg tcgggcgcat acaaatgccg ggcgaagtcg gactcgtccc cgtccgtgaa
     2761 ggttcgcgcc gcgtccacga tctccgcccg cccgccgtag ttgaaggcca cgaacagggt
     2821 gatgcgggtg ttgtcggcgg tggtctgctc ggcccagtcc atcttctcct ggagctcctc
     2881 cgacacgccc tcgcggcggc cgatgaagcg catccgcacg ccctcctcgt ccagctcggg
     2941 cgtctccacg tcgatccgct ggccgaacat ggccatcagg cccgcgacct cgttctcgga
     3001 ccggttccag ttctcggtcg
//
LOCUS       DASUPW010000137         2206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_309158, whole genome shotgun sequence.
ACCESSION   DASUPW010000137 DASUPW010000000
VERSION     DASUPW010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309158"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..495)
                     /locus_tag="VFY44_03120"
     CDS             complement(292..495)
                     /locus_tag="VFY44_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEX5909458.1"
                     /translation="MPTGTVKWFSDEKGFGFITPDDGGKDLFVHHTGISGEGFKSLTE
                     GAKVNFDAESGDKGPKAVNVTQS"
     gene            632..1492
                     /locus_tag="VFY44_03125"
     CDS             632..1492
                     /locus_tag="VFY44_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HEX5909459.1"
                     /translation="MTGPLLLVDAPYLLYRAFFALPDKITGAEGKPVNALLGSVNAIL
                     SAAQEEGARATICCMGPDAAPYRTDLYPAYHAHRPPMPRELAWQWERAPALYEAMGWA
                     GAYDPELEADDLLGAYALAEEAAGGEAVILTGDRDLFQCATDNVRVALLGGRGKKGPE
                     RIDPAEVRKRYGVDPSQVPDFIALRGDPSDGLPGAKGIGEKRAADILKRSGSLEAALE
                     EVESGDAPSTLRDVADELRAFRDIATLRPPDVERPPDRAIDFAGGAAAARELGMERLA
                     DRLEKEAAAA"
     gene            complement(1489..>2206)
                     /locus_tag="VFY44_03130"
     CDS             complement(1489..>2206)
                     /locus_tag="VFY44_03130"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX5909460.1"
                     /translation="SESPEEYLEKGMDTAMLQAGYRKIAQEHGMPIDTTERPRMMATV
                     TACRAIVATRLNAPDLERRILRALRVLCFEGPLIDEPETIVMAANRAGIDPDELQQWM
                     GTDEVEQVLREDMARAREPMPAARVLDDRLANWSGGRRYTCPSYEVERVSDGVRISVP
                     GFQPFAAYDVLLANLVPDLGRRGEPMGVQEVLAWAGFPLATREVAEICELDDRDAREQ
                     LGHVAIEHHVGADGFWSLEA"
BASE COUNT          302 a          816 c          798 g          290 t
ORIGIN      
        1 cgcccaccgt ccccgccgtc gaccgcgagc cggccgagcg tcccgcgtcg cgccagccgc
       61 ggcgcgagcg cgcacggccg cgccaggatc acgggggcgg ccaggaccgg ccggcctggc
      121 gcccgcagga gggcgatcag cagcagcagc gtccacccgc gcctcccgcc taccgccggc
      181 agcccgcgcc gcagccaggg gctaccgagg cgagccggca ggagtgtgtg agaggcgggg
      241 cgagcgagcc cggccccgcg ccctcaggcg gggagccggg cgctcacagc gttacgactg
      301 ggtgacgttg acggccttgg ggcccttgtc accggactcg gcgtcgaagt tgaccttcgc
      361 gccttcggtg aggctcttga agccctcacc cgagattccc gtgtggtgaa cgaaaaggtc
      421 cttgccccca tcgtccgggg tgatgaaccc gaaacccttc tcatcgctga accacttaac
      481 ggtgccagta ggcatgtgga aaatcctcca cgtctttcat gttcgtgccg accgcgctga
      541 gggaacctga gctaccactg cgagcgcctg cactactcct tcgccgggca ccttcacccg
      601 gccgtacgcc gcagaccgta ccatcgtggc ggtgacgggt ccgctgctcc tggtcgacgc
      661 cccctacctg ctctaccggg cgttcttcgc gctgccggac aagatcaccg gcgccgaggg
      721 caagccggtg aacgcgctcc tgggatcggt gaacgcgatc ctgtcggcgg cccaggagga
      781 gggcgcccgc gccaccatct gctgcatggg cccggacgcc gcgccctacc gcaccgacct
      841 gtaccccgcc taccacgccc accgcccgcc gatgccacgc gagctggcgt ggcagtggga
      901 gcgggcgccc gcgctgtacg aggccatggg ctgggccggc gcctacgacc ccgagctcga
      961 ggccgacgac ctgctcggcg cctacgcgct cgcggaggag gccgccggcg gcgaggcggt
     1021 gatcctgacc ggcgaccgcg acctgttcca gtgcgcgacc gacaacgtgc gcgtggcgct
     1081 gctgggcggc cggggcaaga aggggcccga gcggatcgac ccggcggagg tgcgcaagcg
     1141 ctacggcgtc gacccgtcgc aggtgccgga cttcatcgcc ctgcgcggcg acccctcgga
     1201 cgggctgccc ggcgcgaagg gcatcggcga gaagcgcgcg gccgacatcc tcaagcgctc
     1261 cggctccctg gaggccgcac tggaggaggt cgagtccggt gacgccccgt cgacgctgcg
     1321 cgacgtggcc gacgagctgc gcgcgttccg cgacatcgcg accctgcgcc cacccgacgt
     1381 cgagcggccg cccgaccggg ccatcgactt cgccggcggc gcggccgcgg cgagggagct
     1441 gggaatggag cggctggccg accggctgga gaaggaggcc gcggccgcct aggcctcgag
     1501 cgaccagaag ccgtcggcgc ccacgtggtg ctcgatggcc acgtgcccga gctgctcgcg
     1561 cgcgtcgcgg tcgtccagct cgcagatctc ggccacctcg cgggtggcca gcgggaaccc
     1621 ggcccaggcc aggacctcct ggacgcccat cggctcgccg cgccggccca ggtcgggcac
     1681 gaggttggcc agcagcacgt cgtaggccgc gaagggctgg aacccgggca cggagatgcg
     1741 gacgccgtcg gagacgcgct cgacctcgta cgaggggcag gtgtagcggc gtccgcccga
     1801 ccagttggcc agccggtcgt ccagcacgcg cgcggcgggc atcggctcgc gggcgcgggc
     1861 catgtcctcg cgcagcacct gctcgacctc gtccgtgccc atccactgct gcagctcgtc
     1921 ggggtcgatg ccggcccggt tggccgccat gacgatcgtc tccggctcgt cgatcagcgg
     1981 gccctcgaag cagaggacgc gcagggcgcg caggatccgg cgctcgaggt caggggcgtt
     2041 cagccgcgtg gccacgattg cgcggcaggc cgtgaccgtg gccatcatcc gtggccgctc
     2101 ggtggtgtcg atgggcatgc cgtgttcctg ggcgatcttg cggtagccgg cctgcagcat
     2161 cgcggtgtcc atgcccttct ccaggtactc ctcggggctc tcggac
//
LOCUS       DASUPW010000138         2573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1693751, whole genome shotgun sequence.
ACCESSION   DASUPW010000138 DASUPW010000000
VERSION     DASUPW010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1693751"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1464
                     /locus_tag="VFY44_03135"
     CDS             <1..1464
                     /locus_tag="VFY44_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909461.1"
                     /translation="TYAERAAAQAGARLAHEEAAEVLAGAVRAREGDQPVDQVDLARL
                     LLALAAARMRAGQWDAARETHARAAGAAREARAPALLANAALGHSGGSFERYGTEDPE
                     SVALLREALDALPDEDSRLRAQVLARLSGVLYYSPGSEELTGPAAAEAIAMARRLGDD
                     VALATALTAAQYAYWRPGLAEQRADLADELLEVATRLGDPEAEAGAQAWRVTAVLELC
                     RIEAADAAMARHAELAERLQQPELLVHAAALRSMRALLEGRWEEAEAASQDVLSAGER
                     STAADALQFFGVEMIALRNEQLRMDEVGEQFGRLAREIAALPGWRAPVAWAHVQAGRL
                     DAARAELAELRRGGFAVLPHDANFDAALAIVSHVAGELDDAELAAEVEPLLRPLAQYW
                     VVMGPGPATLGPVAYCLGLLNLILGRLDPAERDFELALAKCRAMGARPYEAHTLLGLS
                     RVARERGDDEAADERRDQAVAMARELGMPRLLRDAGV"
     gene            1534..>2573
                     /locus_tag="VFY44_03140"
     CDS             1534..>2573
                     /locus_tag="VFY44_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5909462.1"
                     /translation="MESATTPEAGTTRPKALDATTMAEAFQTTAQQYADRPAIRTKGD
                     EQSYTWGEYAQKVEELARGLAALGLERGQAMGSMLTNRPEFHFFDTAAMHLGATPFSA
                     YNTYTVDQVTYLIQDSEAKIIVTEKAFLDTILAVRDADTPLETVIVVDGDGENDTLTV
                     AEVIEGGDAGFDFEGAWKGVDPDDVLTLIYTSGTTGPPKGVQLTHDNIVFAIGGIEDI
                     IQFPDGGRVISWLPYAHIAERQCSQYLPMVMGFTTTDCPDPRQVMAYMPEVRPTWFFA
                     VPRIWEKLKAGLEAMVAGEQDEQKKAGLEKAFEVGLKKVKLEQAGEPVPEELAAAYAE
                     LDDKVLSKIRETL"
BASE COUNT          378 a          877 c         1004 g          314 t
ORIGIN      
        1 acctacgcgg agcgggcggc cgcccaggca ggtgcgcggc tggcccacga ggaggcggcg
       61 gaggtgctgg ccggagcggt gcgggccagg gagggcgatc agcccgtgga ccaggtggac
      121 ctggcgcggc tgctgctggc cctggccgct gcgcgtatgc gcgccggtca gtgggatgcc
      181 gcccgcgaga cgcacgcgcg ggccgccggc gccgctcggg aggcgcgagc gcccgcgctg
      241 ctggccaacg cggcgctggg gcactccggg ggctcgttcg agcggtacgg caccgaggac
      301 ccggagagcg tggcgctcct gcgcgaggcg ctggatgcgc tgccggacga ggactcccgt
      361 ctgcgcgccc aggtgctggc gcggctcagc ggcgtcctgt actactcgcc cggatcggag
      421 gagctgaccg gcccggcggc ggcggaggcg atcgccatgg cccgccgcct cggcgacgac
      481 gtggcgctgg ccaccgccct gaccgccgcg cagtacgcct actggcggcc gggcctggcc
      541 gagcagcgtg ccgacctggc ggacgagctg ctggaggtgg ccacgcggct cggcgacccg
      601 gaggccgagg ccggggcgca ggcctggcgc gtgaccgcgg tgctggagct gtgccggata
      661 gaggcggccg acgccgccat ggcccgccac gccgagctgg ccgagcgcct gcaacagccg
      721 gagctgctgg tgcacgcggc tgcgctgcgg tccatgcgcg cactgctgga gggccgctgg
      781 gaggaggccg aggcggcgtc ccaggacgtg ctcagcgcag gcgagcggtc cacggcggcg
      841 gacgcactgc agttcttcgg tgtggagatg atcgcgctgc gcaacgagca gctgcggatg
      901 gatgaggtcg gcgagcagtt cggcaggctg gcgcgggaga tcgcggccct tccgggatgg
      961 cgagcaccgg tggcgtgggc ccacgtgcag gccggccgcc tggacgcggc acgcgccgag
     1021 ctcgccgagc tgcggcgcgg cgggttcgcg gtcctgcccc acgacgccaa cttcgacgct
     1081 gcgctggcca tcgtgagcca cgtcgccggc gagctcgacg acgccgagct ggccgccgag
     1141 gtggagccgc tgctccgccc gctcgcgcag tactgggtgg tcatgggtcc cggccccgcg
     1201 acgctggggc cggtcgccta ctgcctcggg ctgctgaacc tgatcctggg ccggttggat
     1261 ccggccgagc gtgacttcga gctcgcgctg gcgaagtgca gggcgatggg cgccaggccg
     1321 tacgaggccc acacgctcct cgggctgtcc agggtcgcgc gcgagcgagg cgacgacgag
     1381 gcagcggacg agcggcgcga ccaggccgtg gccatggcgc gggagctcgg gatgccccgt
     1441 ctgctgcgcg acgcgggggt gtgaccgcgc acctggccgg ttcccgtcag atagggtcct
     1501 ggctgcccag ccagcagccc tgaggagagg acgatggaga gcgctacgac ccccgaggcg
     1561 gggacgaccc gcccgaaggc cctcgacgcc acgacgatgg ccgaggcgtt ccagacgacg
     1621 gcgcagcagt acgccgaccg gcccgccatc cggaccaagg gcgacgaaca gagctacacg
     1681 tggggcgagt acgcgcagaa ggtcgaggag ctggcccgcg ggctggcggc gctggggctc
     1741 gagcgcggcc aggcgatggg ctcgatgctg accaaccggc ccgagttcca cttcttcgac
     1801 accgcggcga tgcacctggg cgcgacgccg ttctccgcgt acaacaccta cacggtggac
     1861 caggtcacgt acctgatcca ggactccgag gccaagatca tcgtcacgga gaaggcgttc
     1921 ctggacacga tcctggccgt gcgcgacgcg gacacgccac tggagaccgt gatcgtggtc
     1981 gacggcgacg gcgagaacga cacgctgacg gtggccgagg tcatcgaggg cggcgacgcc
     2041 ggcttcgact tcgagggcgc ctggaagggc gtggaccccg acgacgtgct gacgctgatc
     2101 tacacgtcgg gcacgaccgg gccgcccaag ggcgtccagc tgacccacga caacatcgtg
     2161 ttcgccatcg gcggcatcga ggacatcatc cagttccccg acgggggccg ggtgatctcg
     2221 tggctgccgt acgcgcacat cgccgagcgc cagtgcagcc agtacctgcc gatggtcatg
     2281 ggcttcacca ccaccgactg ccccgacccg cgccaggtca tggcctacat gcccgaggtg
     2341 cggcccacgt ggttctttgc cgtgccgcgg atctgggaga agctcaaggc cgggctcgag
     2401 gccatggtgg ccggcgagca ggacgagcag aagaaggccg gcctggagaa ggcgttcgag
     2461 gtcgggctca agaaggtcaa gctcgagcag gccggtgagc ccgtgcccga ggagctggcc
     2521 gccgcctacg ccgagctgga cgacaaggtg ctgtccaaga tccgcgagac gct
//
LOCUS       DASUPW010000139         2093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1693828, whole genome shotgun sequence.
ACCESSION   DASUPW010000139 DASUPW010000000
VERSION     DASUPW010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1693828"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1662
                     /locus_tag="VFY44_03145"
     CDS             <1..1662
                     /locus_tag="VFY44_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX5909463.1"
                     /translation="AHTALGEKTSASIRAWAGPIFVGMTATGALIARHVTDLFPTQTS
                     RFDLAQAARRGVIAPLRCVRIPPGVGVRSISKVPLRKGEVDTEFDQEELAALLDQMPF
                     NLAIADLYKTRFKDLPGVVYTAGVQHAHNVAAAFQDQGIKAVAVSGETPKRELTRTLA
                     AYERGEIDVLCNAQLLAEGWNSPRATVCMHLAPTASRRIYQQRVGRVTRRQPGKEAGI
                     VVDFVHPATTNDDPVVTLHSLLDRDVYRGGAIVVGPVRRGRGRRLRVERRVLPVTSDE
                     ERRAQVFERELWRIAVEHLSWGEQHAWSALAGARVSSNNWRRAKAMLHFDNTGELRRR
                     YLVTCLQRNRNTQLRLRALQEVGQLKDAEAFDEAVELVGGWSRDERREGVKVLLQAMA
                     EKPLGRRDQAARWIWHLAEYSREVHEEYAVSRWPETKRLLGLLVNSSGGAHARNARRL
                     VQASKKQDRRLSAAILAAALAHTPEAQQVLDGARMRMARKPSALARELLKNFPKGRRR
                     RGGRRRRGRGKGGGGDGAEVDAQATQNAEKQGGKGKGDAQERDAA"
     gene            complement(1663..>2093)
                     /locus_tag="VFY44_03150"
     CDS             complement(1663..>2093)
                     /locus_tag="VFY44_03150"
                     /inference="COORDINATES: protein motif:HMM:NF014310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909464.1"
                     /translation="GGPGGASPLVGIKWPNDVLVDGRKVCGILVEGRPQEQWAVLGIG
                     LNVLPVEFPPDLNAAALDDGRSVEQVLAALLERLEQRLTGADALEAWRERDTLLGRRI
                     SWADGAGTAAGVDESGALLVDTAGGRVALDAGEVHLDPLG"
BASE COUNT          290 a          805 c          750 g          248 t
ORIGIN      
        1 gcgcacacgg cgctgggcga gaagacctcc gcctcgatcc gcgcctgggc cggcccgatc
       61 ttcgtcggca tgaccgccac cggcgcgctc atcgcccgcc acgtcaccga cctcttcccc
      121 actcagacgt cgcgcttcga cctcgcgcag gccgcccgcc ggggcgtgat cgcgccgctt
      181 cgctgcgtgc gcatcccgcc gggcgtgggc gtgcgctcga tctcgaaggt gccgctgcgc
      241 aagggcgagg tcgacacgga gttcgaccag gaggagctgg ccgcgctgct ggaccagatg
      301 cccttcaacc tggccatcgc cgacctgtac aagacgcgct tcaaggacct ccccggcgtg
      361 gtctacacgg cgggcgtcca gcacgcccac aacgtggctg ccgccttcca ggaccagggc
      421 atcaaggcgg tcgccgtgtc cggcgagacg cccaagcgcg agctcacccg cacgcttgcc
      481 gcctacgagc gtggcgagat cgacgtgctg tgcaacgccc agctgctggc cgagggctgg
      541 aactcgcccc gggccaccgt ctgcatgcac ctggccccga ccgcgtcgcg ccgcatctac
      601 cagcagcgcg tgggccgcgt gacccggcgc cagccgggca aggaggcggg catcgtcgtg
      661 gacttcgtcc acccggcgac caccaacgac gaccccgtgg tcaccctgca ctcgctgctg
      721 gaccgcgacg tctaccgcgg cggcgccatc gtcgtcgggc ccgtgcgccg cggccgcggc
      781 cggcgcctgc gcgtcgagcg ccgcgtcctg ccggtgacct ccgacgagga gcgccgcgcg
      841 caggtcttcg agcgcgagct gtggcggatc gccgtggagc acctgagctg gggcgagcag
      901 cacgcctggt cggccctggc cggcgcgcgc gtgtcgtcca acaactggcg ccgcgcgaag
      961 gcgatgctgc acttcgacaa caccggtgag ctgcgccgcc ggtacctggt cacctgcctc
     1021 cagcgcaacc gcaacaccca gctgcgcctg cgcgcgctcc aggaggtcgg gcagctcaag
     1081 gacgccgagg ccttcgacga ggcggtcgag ctggtgggcg gctggtcgcg cgacgagcgc
     1141 cgcgagggcg tgaaggtgct gctgcaggca atggccgaga agccgctggg ccgccgtgac
     1201 caggcggccc gctggatctg gcacctggcc gagtactcgc gtgaggtgca cgaggagtac
     1261 gccgtgtcgc gctggcccga gaccaagcgc ctgctcgggc tgctggtcaa ctcctccggg
     1321 ggagcgcacg cgcgcaacgc gcgccggctg gtgcaggcct ccaagaagca ggaccggcgc
     1381 ctgtcggccg ccatcctggc tgcggcgctg gcgcacacgc ccgaggccca gcaggtgctg
     1441 gacggcgcgc gcatgcgcat ggcgcgcaag cccagcgcgc tggcccgcga gctgctgaag
     1501 aacttcccca agggccgtcg caggcgcggc ggccggcgac ggcgcggccg cggcaagggc
     1561 ggcggcggtg acggcgccga ggtggacgcc caggccacgc agaacgccga gaagcagggc
     1621 ggcaagggca agggcgacgc gcaggagcgc gacgcggcct agctagccga gcgggtccag
     1681 gtggacctcg ccggcgtcca gtgcgacccg gccgccggcc gtgtccacca gcagcgcgcc
     1741 ggactcgtcc acgccggcgg ccgtgcccgc gccgtccgcc cacgagatcc ggcgccccag
     1801 cagcgtgtcg cgctcgcgcc aggcctcgag cgcgtctgcg ccggtcagcc gctgctcgag
     1861 ccgttccagc agcgcggcca ggacctgctc gacgctccgg ccgtcatcca gtgcggcggc
     1921 gttcaggtcc ggcgggaact ccaccggcag gacgttcagg ccgatgccca gcaccgccca
     1981 ctgctcctgc gggcggccct cgaccaggat cccgcacacc ttgcgcccat cgaccagcac
     2041 gtcgttgggc cacttgatgc cgaccagggg gctcgcgccc cccggacccc ccg
//
LOCUS       DASUPW010000140         4121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1555626, whole genome shotgun sequence.
ACCESSION   DASUPW010000140 DASUPW010000000
VERSION     DASUPW010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555626"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VFY44_03155"
     CDS             complement(<1..407)
                     /locus_tag="VFY44_03155"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909465.1"
                     /translation="MQHREDGVAPQEPAALAAAFEHDPLVVVEAMSHGMEVECSVLGN
                     LEPEASVPGEIVLNADWYDYEAKYQAGGMELVAPARLPEAVRDRVRALAVDAFRRSDC
                     AGLARCDFFVEDPEGEARVLVNELNTMPGFTAT"
     gene            342..1166
                     /locus_tag="VFY44_03160"
     CDS             342..1166
                     /locus_tag="VFY44_03160"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEX5909466.1"
                     /translation="MLERGGQGGGLLGCDAVFPVLHGAYGEDGVVQGLLEALDVPYVG
                     AGVMSSAVCMDKVVFKELMAAAGVPQVAYAAVREGDDPAPLAELGLPVFVKPSRMGSS
                     VGISKVTDAGALPAALAAAFEHDPLVVVEAMSHGMEVECSVLGNLDPEASVPGEIVLN
                     ADWYDFEAKYQSGGMELVAPARLPEPVRDRVRALAVDAFRRSDCAGLARCDFFVEDPE
                     GDARVLVNELNTMPGFTATSVYAKLWQATGLEYGPLLDRLLELAVERHRRERGYRF"
     gene            complement(1163..2641)
                     /locus_tag="VFY44_03165"
     CDS             complement(1163..2641)
                     /locus_tag="VFY44_03165"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5909467.1"
                     /translation="MIGTLLNERFRLEEKIGSGGMSTVYRAWDPTLERWVAIKLMHRD
                     ISEEPDQLERFRREARAVARLSHPHVVTVIDFGEDEGTPYIVLEYVEGETLKDRIKRM
                     GRLPVAEAVAYGIEIGRALTCAHTAHLVHRDVKPQNVLIDMEGRAKVTDFGIARSLEV
                     DVAGLTADGRVLGTTDYVSPEQAMGKEVTEQSDVYSLGIVMFEMLTGEVPFRAESQVA
                     VAMKHVKEPIPDVQRIRPEASATLASVVEHATAKELVNRYATAEDMTEDLEEVLAIEV
                     ARAGEAGSEATTVLRALDSDTKGFVPVRLRNPRRWLLSVLAVLILAAGAVAYFASRTE
                     KGAGPPSTPRPAGLTAVSLGSDAANDYDPEGDDRESPESPQNVLDRNPSTQWDTERYD
                     SGLEGVGKSGVGLFVDAGRPVEARALKVTTSTPGFTAAVYASNEVPDEIGGWKRVAKP
                     TRVDEEQTIRFDDPGRYRRYLLWITKLPEGNKAALQEVNLYR"
     gene            complement(2881..3948)
                     /gene="purM"
                     /locus_tag="VFY44_03170"
     CDS             complement(2881..3948)
                     /gene="purM"
                     /locus_tag="VFY44_03170"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX5909468.1"
                     /translation="MADSAYARAGVDTGQAGAGVAALVGVLKTIDTGRQSRALLGSGH
                     YANVLRLSGDAGLALSTDGVGTKLIVAEQMGRYDTVGIDCIAMNVNDVICVGADPIAV
                     LDYVAIEQADPALLEAVGVGLKAGAEDAGVEIPGGELAVVPELIRGHPSPGGIDLVGM
                     CVGLVDIPRIVTGASVAPGDAIVGLPASGVHSNGFTLARKALTDLDETPPELDGASVG
                     EALLAPTVIYVRATRDLLASGLDVRGLAHITGEGLLNLLRLEADVGYRIDAPLPVLPI
                     FDLIADRSSTEPAEMHQVFNMGCGFCVVVPAEQADDAVALLAQRHPGAAVIGSVTDRA
                     GLVEVPGDGLAGREGQGFGPA"
BASE COUNT          539 a         1494 c         1485 g          603 t
ORIGIN      
        1 ctggtggcgg tgaagccggg catcgtgttc agctcgttga ccaggacccg ggcctcgccc
       61 tcggggtcct cgacgaagaa gtcgcaccgc gcaaggccgg cgcagtcgga gcggcggaag
      121 gcgtccacgg ccagggcccg cacgcggtcg cgcaccgcct cgggcaggcg cgccggggcc
      181 accagctcca tgccccccgc ctggtacttg gcctcgtagt cgtaccagtc ggcgttcagc
      241 acgatctcgc ccgggaccga ggcctcgggc tccaggttgc ccagcaccga gcactccacc
      301 tccatgccgt gcgacatggc ctcgaccacg accagcgggt cgtgctcgaa cgcggcggcc
      361 agggcggcgg gctcttgggg tgcgacgccg tcttcccggt gctgcacggc gcctacggcg
      421 aggacggggt ggtccagggg ctcctggagg cgctggacgt gccctacgtg ggtgccgggg
      481 tcatgtcctc ggccgtgtgc atggacaagg tggtgttcaa ggagctgatg gccgccgcgg
      541 gcgtgcccca ggtggcctac gccgccgtgc gcgagggcga cgaccccgcc ccgctcgcgg
      601 agctcggcct gccggtgttc gtcaagccgt cgcggatggg ctcgtccgtg ggcatctcga
      661 aggtcaccga cgccggcgcg ctgcccgccg ccctggccgc ggcgttcgag cacgacccgc
      721 tggtcgtggt cgaggccatg tcgcacggca tggaggtgga gtgctcggtc ctgggcaacc
      781 tggaccccga ggcctccgtg ccgggcgaga tcgtcctgaa cgccgactgg tacgacttcg
      841 aggccaagta ccagtcgggc ggcatggagc tggtggcccc cgcgcgcctg cccgagccgg
      901 tgcgcgaccg cgtgcgggcc ctggccgtgg acgccttccg ccggtccgac tgcgccggcc
      961 tggcgcggtg cgacttcttc gtcgaggacc ccgagggcga cgcccgcgtc ctggtcaacg
     1021 agctgaacac gatgcccggc ttcaccgcca ccagcgtgta cgcgaagctc tggcaggcca
     1081 ccgggctgga gtacggcccg ctgctggacc ggctgctcga gctggccgtc gagcgccacc
     1141 gtcgcgagcg cggctaccgc ttctagcggt agaggttgac ctcctggagc gccgccttgt
     1201 tgccctcggg cagcttggtg atccacagca ggtagcgccg gtagcgcccg ggatcgtcga
     1261 agcggatggt ctgctcctcg tcgacacggg tcggcttggc cacgcgcttc cagccgccga
     1321 tctcgtccgg cacctcgttc gaggcgtaca cggcggccgt gaacccgggc gtggacgtcg
     1381 tgaccttcag ggcgcgggcc tccacgggcc ggccggcgtc cacgaacagg ccgacgccgg
     1441 acttgcccac gccctcgagc ccgctgtcgt agcgctcggt gtcccactgc gtcgacgggt
     1501 tgcggtccag gacgttctgg ggcgactcgg gcgactcgcg gtcgtcgccc tcggggtcgt
     1561 agtcgttcgc ggcgtcggag cccagcgaca ccgcggtcag gccggcgggc cgcggggtcg
     1621 agggcgggcc ggcgcccttc tcggtgcgcg aggcgaagta ggccacggcg ccggcggcca
     1681 ggatgagcac ggccaggacc gagagcagcc accggcgcgg gttgcgcagc cgcaccggca
     1741 cgaagccctt ggtgtcgctg tccagggcgc gcaggacggt cgtggcctcg gacccggcct
     1801 cgccggcgcg ggcgacctcg atggccagca cctcctccag gtcctcggtc atgtcctccg
     1861 cggtggcgta gcggttgacc agctccttgg ccgtggcgtg ctcgaccacc gaggccagcg
     1921 tggccgacgc ctcgggccgg atccgctgca cgtcggggat cggctccttg acgtgcttca
     1981 tggccaccgc cacctggctc tcggcccgga acggcacctc gccggtgagc atctcgaaca
     2041 tcacgatgcc cagcgagtac acgtcggact gctcggtgac ctccttgccc atggcctgct
     2101 cgggcgagac gtagtcggtg gtgcccagca cgcggccgtc ggcggtcagg cccgccacgt
     2161 ccacctccag cgaccgcgcg atgccgaagt cggtgacctt ggcgcggccc tccatgtcga
     2221 tcagcacgtt ctggggcttg acgtcccggt gcaccaggtg cgcggtgtgc gcgcaggtca
     2281 gcgcgcgccc gatctcgatg ccgtaggcca ccgcctcggc caccggcagg cgtcccatgc
     2341 gcttgatccg gtccttcagc gtctcgccct cgacgtactc gagcacgatg tacggcgtgc
     2401 cctcgtcctc gccgaagtcg atgacggtga ccacgtgtgg gtgcgacagc cgcgccaccg
     2461 cgcgcgcctc gcggcggaac cgctccagct ggtcgggctc ctcggagatg tcccggtgca
     2521 tcagcttgat ggccacccac cgctccagcg tgggatccca cgcccggtag acggtggaca
     2581 tcccgccgga gccgatcttc tcctccaggc ggaagcgttc gttgaggagg gtgccgatca
     2641 cgctggtctc attgtgagtg attcgcccga tggggctggt ccggagttcg gcgcggccgg
     2701 gcggcgccct tttccaccag gccgcttcgt gggagcaacg tggtgcccgc ccaacccacg
     2761 ctcgcccttt tcagaaattc cttgccatgg gatggcaacg gcgaccaccg ggtggcaaga
     2821 agtttctgaa aacgccaagc gggggctcgc ggtggatccc cgcccggcct gcaacggggc
     2881 tcaggccggg ccgaagccct ggccctcgcg ccccgcgagc ccgtcgcccg ggacctccac
     2941 gaggcccgcg cgatcggtca ccgagccgat cacggcggcg cccgggtggc gctgggcgag
     3001 gagcgcgacc gcgtcgtcgg cctgctcggc cgggaccacc acgcagaagc cgcagcccat
     3061 gttgaagacc tgatgcatct cggccggctc ggtggaggag cggtcggcga tcaggtcgaa
     3121 gatcggcagc acgggcagcg gcgcgtcgat ccggtagccc acgtcggctt ccaggcgcag
     3181 caggttcagc agcccctcgc cggtgatgtg cgccagcccg cgcacgtcca ggcccgaggc
     3241 cagcaggtcg cgcgtggcgc gcacgtagat gaccgtcggc gccagcagcg cctcgcccac
     3301 ggatgcgccg tccagctcgg ggggcgtctc gtccaagtcg gtgagcgcct tgcgcgccag
     3361 cgtgaagccg ttggagtgca cgccgctggc gggcaggccc acgatggcgt cgcccggggc
     3421 caccgaggcg ccggtgacga tccgggggat gtccaccagc ccgacgcaca tgcccaccag
     3481 gtcgatgccg cctggggagg ggtggccgcg gatcagctcg ggcacgacgg cgagctcacc
     3541 gcccgggatc tccacgcccg cgtcctcggc accggccttc aggcccacgc cgacggcctc
     3601 gagcagcgcg ggatcggcct gctcgatggc cacgtagtcc aggacggcga tcgggtcggc
     3661 gccgacgcag atcacgtcgt tcacgttcat ggcgatgcag tcgatgccga cggtgtcgta
     3721 gcggcccatc tgctcggcga cgatgagctt ggtgcccacg ccgtcggtgg acagcgccag
     3781 cccggcgtcg ccggacaggc gcagcacgtt ggcgtagtgg ccagagccca gcagggcccg
     3841 gctctggcgc ccggtgtcga tcgtcttgag cacgccgacc agcgcggcca cgccggcgcc
     3901 ggcctggccg gtgtccacgc ccgcgcgtgc gtatgccgaa tcagccatcg gcgggggagt
     3961 taatcagcgt tgacgtaccg ccagggccgt ggagtcgtcc accaggatgc cggcccagcc
     4021 gcgcaccgcc tcgtggaccg cgtgcacgag ctcctcgatc gacccgccgg tgccgcgctg
     4081 gtcgtccagg gcctcgcgca gacgaccctc tccgagcatc t
//
LOCUS       DASUPW010000141         3783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1279169, whole genome shotgun sequence.
ACCESSION   DASUPW010000141 DASUPW010000000
VERSION     DASUPW010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279169"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VFY44_03175"
     CDS             <1..612
                     /locus_tag="VFY44_03175"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX5909469.1"
                     /translation="QASVYAGQLGKQLAERGVTAYDDGAKANAWGSDGIDDEGTPTQR
                     TTVIDDGELVSYLYDVQSARQDGVASTGNGRRQSFRHLPIPRMTNTYFAPGEASADEL
                     IASIDRGLYAVSFGGGQVEPATGDFVFGVSEGYLIEDGKVTAPVRGATLVGNGLQALA
                     AVDGIARDLDIATGYCGKAGQSVPAGVGQPHVRIRELTVGGTG"
     gene            609..1958
                     /locus_tag="VFY44_03180"
     CDS             609..1958
                     /locus_tag="VFY44_03180"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX5909470.1"
                     /translation="MSDLGALARQGVEAALAAGAGDAEAWVESSTSRQIRVYDGEVES
                     LSDSGGRGIGIRAFIDGRTGYSYGTDLEPGGLKAVAERAHALAAVADPDEFAGLPDET
                     GTTDVAGLESAAMADWDTERKVELALAVDRAARSRPGVSQVENTVYSDGEGTSAIANS
                     RGFTASTASTQAWAYSSAFAGEGQDLMTGMGIGLGRDPGELDAEAIGHEAADRALALV
                     GARQPSSRRCPVVLDAFVAASFAGFIGAMLSADAVQRGRSLFAGREGDEVAEAAFALT
                     DDGRHPDGPASGPFDGEGAATRRTALIEGGRVAGYLYDSRTARKDGRATTGNAGRGSY
                     RSQPSVGTHNLIVEPGSGSLDDLVAQAGEGLYVTEVSGLHSGVNPVSGTFSVGASGRL
                     IEGGELGTPVREITIASDLVGMLKAVLAVGGQPRWIPLGGSVQAVPLLIGEMSVSGS"
     gene            2080..2358
                     /locus_tag="VFY44_03185"
     CDS             2080..2358
                     /locus_tag="VFY44_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003215863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX5909471.1"
                     /translation="MTKSEFVDQVASESGLSKGDAGDAVDAVLTVIEGTLKRGGEINF
                     TGFGKFSVADRGARQGVNPQTGEKIQIAASKVPKFSAGSALKKSVKGG"
     gene            2366..3283
                     /gene="pyrF"
                     /locus_tag="VFY44_03190"
     CDS             2366..3283
                     /gene="pyrF"
                     /locus_tag="VFY44_03190"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX5909472.1"
                     /translation="MPEHFADRLAALVEERRSRVVLGLDPDPAKLWPGAVREIDAGGA
                     PGDAAAEAAEAVALHCRAAIEAAGPSCVAVKPQLACFERLGAAGWAALNATRDAAHEA
                     GLLVLADGKRGDVPVTAAAYAQALVGSTPTPYGPVQGLGADCFTVNPLLGRDSLEPLV
                     DAAAEHGAGVFALVSTSNPGAADRQDLDAGGRPLHEHVAALVDSIGAAHVGQCGLSLA
                     GAVTGATRPERLARLRELMPRAVFLLPGVGAQGGDPAALADAFRPHPAAGLVTASRSI
                     VNAYADRGGDPAVAARDAAAELQKTTWAL"
     gene            3330..3665
                     /locus_tag="VFY44_03195"
     CDS             3330..3665
                     /locus_tag="VFY44_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEX5909473.1"
                     /translation="MAHQPSRSPVRLLAPVALVLTFLAFAFVVLSSREDNDAAPASDT
                     STAPKSDTQASTAPKPKPKPATYTVKAGDNLGSISEKTQVPIETLQELNPELVPQALV
                     SGQKIKLKE"
BASE COUNT          510 a         1423 c         1376 g          474 t
ORIGIN      
        1 caggccagcg tctacgccgg ccagctgggc aagcagctgg ccgagcgcgg cgtgaccgcc
       61 tacgacgacg gggccaaggc gaacgcgtgg ggcagcgacg gcatagacga cgagggcacg
      121 ccaacccagc gcaccacggt catcgacgac ggcgagctgg tctcctacct ctacgacgtc
      181 cagagcgcgc gccaggacgg cgtcgcctcc accggcaacg gccgccggca gtcgttccgc
      241 cacctgccga tcccgcggat gaccaacacc tacttcgccc ctggcgaggc cagcgcggac
      301 gagctgatcg cctcgatcga ccggggcctg tacgcggtct ccttcggcgg cggccaggtg
      361 gagccggcca ccggcgactt cgtgttcggg gtcagcgagg gctacctgat cgaggacggc
      421 aaggtcacgg cgccggtgcg cggggccacg ctggtgggca acggcctgca ggcgctggcc
      481 gcggtggacg gcatcgcgcg cgacctggac atcgccaccg gctactgcgg caaggcgggc
      541 cagagcgtgc ccgccggcgt cggccagccg cacgtgcgca tccgcgagct caccgtgggc
      601 ggcaccggat gagcgacctc ggggcgctgg cccgccaggg cgtcgaggcc gcgctcgccg
      661 cgggcgccgg cgacgccgag gcctgggtcg agagctccac cagccgccag atccgcgtct
      721 acgacggcga ggtcgagagc ctcagcgact cgggcgggcg cgggatcggc atccgggcgt
      781 tcatcgacgg gcggaccggc tactcgtacg gcaccgacct ggagcccggc gggctgaagg
      841 cggtcgccga gcgcgcgcac gcgctggccg cggtggccga ccccgacgag ttcgccggcc
      901 tgcccgacga gaccgggacc accgacgtcg cgggcctgga gtcggccgcc atggccgact
      961 gggacaccga gcgcaaggtc gagctggcgc tggccgtcga ccgcgccgcc cgctcgcgcc
     1021 ccggcgtcag ccaggtcgag aacaccgtct actccgacgg cgagggcacg tcggccatcg
     1081 ccaactcgcg cggcttcacc gccagcaccg cctccaccca ggcgtgggcc tactcgtccg
     1141 ccttcgcggg cgagggccag gacctgatga cggggatggg catcgggctg ggccgcgacc
     1201 ccggcgagct ggacgccgag gccatcggcc acgaggcggc cgaccgcgcg ctggccctgg
     1261 tcggcgcgcg ccagccctcg agccggcgct gcccggtggt gctcgacgcg ttcgtggccg
     1321 cctcgttcgc gggcttcatc ggcgcgatgc tgtcggccga cgcggtccag cgcgggcggt
     1381 ccctgttcgc cggccgcgag ggcgacgagg tggccgaggc ggcgttcgcg ctgaccgacg
     1441 acggccgcca ccccgacggc ccggccagcg ggccgttcga cggcgagggg gccgccacgc
     1501 gccggaccgc cctgatcgag ggcggtcgcg tcgcggggta cctgtacgac tcgcgcacgg
     1561 cgcgcaagga cggtcgcgcc acgaccggga acgcgggccg cggctcctac cgctcgcagc
     1621 cctcggtggg cacccacaac ctgatcgtgg agccgggcag cggcagcctg gacgacctgg
     1681 tcgcccaggc cggcgagggc ctgtacgtga ccgaggtctc gggcctgcac tcgggcgtga
     1741 acccggtctc ggggacgttc tccgtgggcg cctcgggacg gctgatcgag ggcggcgagc
     1801 tgggtacgcc ggtgcgggag atcaccatcg ccagcgacct ggtgggcatg ctcaaggcgg
     1861 tgctcgcggt ggggggccag ccgcgctgga taccgctcgg aggcagtgtt caagcggttc
     1921 cgctgctcat cggcgagatg tccgtttcgg ggtcttgacc ccgcattttc cggctttccc
     1981 ggcccgcaaa tccccttcta aagccaaatc cgggcgggaa tcgtccgcga gcgccgttat
     2041 gttcgccccg atgtcgattc ccccgagaag gaggacaaca tgacgaagtc ggaattcgtc
     2101 gaccaggtgg ccagcgagtc tggcctttcg aagggcgacg cgggcgatgc agttgacgcc
     2161 gtcctgaccg tcatcgaggg cacgctgaag cgtggtgggg agatcaactt caccggcttc
     2221 ggcaagttct ctgtggctga tcgcggcgcc cgccagggtg tcaacccgca gaccggcgag
     2281 aagatccaga tcgccgcgag caaggtgccg aagttctcgg ccggtagcgc cctgaagaag
     2341 tcagtcaagg gcggctagct cacgcgtgcc tgagcacttt gccgaccggc tggcggcgct
     2401 cgtagaggag cgtcgcagcc gtgtcgtgct cgggctcgac ccggacccgg ccaaactgtg
     2461 gccgggcgcc gtccgcgaga tcgatgcggg cggcgcaccg ggcgacgcgg cggccgaggc
     2521 cgccgaggcg gtggccctcc actgccgcgc ggccatcgag gccgcgggac cgtcctgtgt
     2581 ggcggtcaag ccccagctgg cctgcttcga gcggctgggt gccgcgggct gggcagcact
     2641 caacgccacc cgcgacgcgg cccacgaggc cgggctgctg gtgctggccg acggcaagcg
     2701 cggcgacgtc cccgtgaccg ccgccgccta cgcgcaggcg ctggtgggct ccacgcccac
     2761 cccgtacggc ccggtccagg gcctcggcgc cgactgcttc accgtcaatc ccctcctggg
     2821 ccgcgactcg ctggagccgc tggtggacgc cgcggccgag cacggcgcgg gcgtgttcgc
     2881 gctggtcagc acctccaacc ccggcgccgc cgaccgccag gacctcgacg ccggcgggcg
     2941 cccgctgcac gagcacgtgg ccgccctggt ggactccatc ggcgccgccc acgtgggcca
     3001 gtgcggcctg tccctggccg gggcggtcac cggcgccacc cggcccgagc ggctggcgcg
     3061 cctgcgcgag ctgatgccgc gggccgtgtt cctgcttccc ggcgtgggtg cccagggtgg
     3121 cgaccccgcc gccctcgccg acgccttccg cccgcacccg gcggcgggcc tggtgaccgc
     3181 ctcgcgctcg atcgtgaacg cctacgcgga ccgcggaggc gatccggcgg tggcggcgcg
     3241 cgacgcggca gcggaactgc agaagaccac ctgggcgctc tagcgcccta ttctccgcct
     3301 ctgcaccccc ctcggggcgc caccccgcca tggcccatca accttcacgc agccccgtcc
     3361 gcctgctggc gccggtcgcg ctcgtcctga cgttcctcgc gttcgcgttc gtggtcctca
     3421 gctcgcgcga ggacaacgac gccgcgcccg cgtccgacac ctcgaccgcg cccaagtcgg
     3481 acacgcaggc cagcaccgcc cccaagccca agcccaagcc ggccacctac accgtgaagg
     3541 cgggcgacaa cctggggtcg atatccgaga agacccaggt cccgatcgag accctgcagg
     3601 agctcaaccc cgagctcgtc ccgcaggcgc tcgtgtccgg gcagaagatc aagctcaagg
     3661 agtgactgcc cggcctctca gggccgtgct ggtggccctc gtggcgctgg cgctggctcc
     3721 gctcgcccct gcccgcgcgg ccgaccccaa gcccggcctg gacgccgccg ccagcgcgat
     3781 cct
//
LOCUS       DASUPW010000142         3622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_586876, whole genome shotgun sequence.
ACCESSION   DASUPW010000142 DASUPW010000000
VERSION     DASUPW010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586876"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VFY44_03200"
     CDS             <1..654
                     /locus_tag="VFY44_03200"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5909474.1"
                     /translation="WLVTLPLDRRKVVLHLYSCAWAVFFVALNPLWRLNVTRRDLLPW
                     KGPAVIVANHASLVDILVVFDLFRPFKWVSKIENFKIPFVGWNMRLNGYVPLLRGDRD
                     SVVAMLARCSELLGKGNPVLFFPEGTRSKDGQMRPFKDGAFELAMEHRVPLIPVSVHG
                     TSRALPKHGMVLKEHVDAHVDVLEPMHPADYASVAELRDAARERIAVALAERDEDHS"
     gene            710..1204
                     /locus_tag="VFY44_03205"
     CDS             710..1204
                     /locus_tag="VFY44_03205"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX5909475.1"
                     /translation="MSATLEVTVQEPSELTGNLGWLLAQASHALSTELTAAFEAAGGT
                     PRGHCVLSTASTGAFTQKELADRIGLDKTTMVVTLDALEATGLARRVPSPHDRRARVI
                     EVTAAGKKAVREGEKVVQRIQEDVLASLPADEREALMEALAKLVCGPLSAPSACERAP
                     RRKA"
     gene            1327..2826
                     /locus_tag="VFY44_03210"
     CDS             1327..2826
                     /locus_tag="VFY44_03210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5909476.1"
                     /translation="MTAPPETEAKPRTQREKMLALYVLCTGMLMIILDGTIVNVALPS
                     IQSDLNFSQTDLAWVVNAYLIPFGGLLLLSGRLGDLIGRKRLFTSGLVLFVAASALCG
                     LASSQAMLVGARFLQGVGGALTSAVILGMIVTMFPGAREQAKAIGVYSFVASAGASIG
                     LLLGGVLTEAISWHWIFFVNLPIGLAALAAAHRLIDSDEGIGLSRGADVPGAVLVTGS
                     LMLGVYTIVGIPEEGWSSLQTLGLGAVSAALMAAFVARQHTAKTPLFPLRILRSRNVT
                     GANVVQVLMVAGLFGMFFLGALYLQGIQGYSPIEVGLAYLPVALLIGGVSLKLADKLI
                     LRFGARRVLIAGLTSVLGGLLLFTRAPLEATYVTDILPTMVLFGIGAGLSFPAMVSLA
                     MSGATQEDAGLASGLVNTTQQVGGALGLAVLATLSTTRTEDKLADGASQASALVDGYQ
                     LAFFVGAGFAAAAIAFAVTVLEPTPELEIEELPDVELEEGVEVGYREAA"
     gene            2851..3582
                     /locus_tag="VFY44_03215"
     CDS             2851..3582
                     /locus_tag="VFY44_03215"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX5909477.1"
                     /translation="MSAGALDLQQILQRLRASEWVDLTHSFAPGVPHYHQFPDEQREQ
                     LFDYSDGFRVHEYRHVGQWGTHVDPPSHFIEGGRTLDQLPVTDMVLELVVLDARSRVE
                     ADPDYAADAALVRAHEEEHGPVPEGAFVALRTGWSERWPDPEAFDNGGRRPGWDVSAL
                     EVLVDGRGIAAIGHETTDTDPAAVLAAGSTAAETLILDRDRWQIEGMASLDRLPARGA
                     LIVAAWPKPAGGSGFPARCFAILPS"
BASE COUNT          488 a         1285 c         1288 g          561 t
ORIGIN      
        1 tggctggtca cgctgccgct ggaccggcgc aaggtggtgc tccacctgta ctcctgcgcg
       61 tgggccgtgt tcttcgtggc cctgaacccg ctgtggcgcc tgaacgtgac ccggcgcgac
      121 ctgctgccgt ggaagggccc ggcggtgatc gtggccaacc acgcgtccct ggtggacatc
      181 ctggtggtgt tcgacctgtt ccggccgttc aagtgggtgt ccaagatcga gaacttcaag
      241 atcccgttcg tgggctggaa catgcgcctg aacggctacg tgcccctgct gcgcggcgac
      301 cgcgactcgg tggtggccat gctggcccgc tgctcggagc tgctgggcaa gggcaacccg
      361 gtgctgttct tccccgaggg gacccgcagc aaggacgggc agatgcgccc gttcaaggac
      421 ggcgcgttcg agctggccat ggagcaccgc gtgccgctga tcccggtgtc ggtgcacggc
      481 acgtcccgcg cactgcccaa gcacggcatg gtgctcaagg agcacgtcga cgcccacgtg
      541 gacgtgctgg agccgatgca cccggccgac tacgcctcgg tggccgagct gcgcgacgcc
      601 gcgcgcgagc ggatcgcggt ggcgctggcc gagcgcgacg aggaccactc gtagatcgtt
      661 ccgtaccaga tcgtccgata gcggacctat cgctggtacc cttgatgcca tgagcgccac
      721 cctcgaggtc acggtccagg agcccagcga gctcacgggc aacctcggat ggctgctggc
      781 ccaggccagc cacgcgctct cgaccgagct gaccgcggcc ttcgaggcgg cgggcggcac
      841 gccccgcggg cactgtgtgc tgtccaccgc cagcaccgga gcgttcaccc agaaggagct
      901 ggccgaccgg atcggcctgg acaagaccac gatggtggtc accctggacg ccctcgaggc
      961 cacggggctg gcgcggcgcg tgccctcccc ccacgatcgc cgcgcacgcg tgatcgaggt
     1021 cacggccgcc ggcaagaagg cggttcgcga gggcgagaag gtcgtccagc ggatccagga
     1081 ggacgtgctg gcaagcctgc cggccgacga gcgcgaggcg ctcatggaag cgctggccaa
     1141 gctggtctgc gggccgctct cggccccctc ggcgtgcgag cgcgcgccgc gccgcaaggc
     1201 ctgaagcacc ctcgcagtac gggtaacccc ccaatcggtc caaccgagat agtccgctac
     1261 ggatctatct gctacggtcg gatctcaact tactcttcgt aaggagcaca ccgtatgagc
     1321 gccaccatga ccgccccacc agagaccgaa gccaagccgc gtacccagcg ggagaagatg
     1381 ctggcgctgt acgtgctgtg caccggcatg ctcatgatca tcctggacgg gacgatcgtg
     1441 aacgtggcgc tgccctcgat ccagtccgac ctgaacttct cgcagaccga cctggcgtgg
     1501 gtggtcaacg cgtacctgat cccgttcggc ggcctcctgc tgctgtccgg ccgcctcggt
     1561 gacctgatcg ggcgcaagcg cctgttcacc tcgggcctgg tgctgttcgt ggccgcctcc
     1621 gcgctctgcg gcctggcctc cagccaggcg atgctggtcg gcgcgcgctt cctgcagggc
     1681 gtcggcgggg cgctgacctc cgcggtgatc ctgggcatga tcgtgaccat gttcccgggc
     1741 gcccgggagc aggcgaaggc catcggcgtg tacagcttcg tggcctccgc gggcgcctcc
     1801 atcggcctgc tgctgggcgg cgtgctgacc gaggccatca gctggcactg gatcttcttc
     1861 gtgaacctgc cgatcggcct ggccgcgctg gccgccgcac accggctgat cgactccgac
     1921 gagggcatcg gcctctcgcg cggcgccgac gtgccgggcg cggtgctggt gaccggctcg
     1981 ctgatgctcg gcgtgtacac catcgtcggg atccccgagg agggctggag ctcgctgcag
     2041 acgctgggcc tcggcgccgt gtccgcggca ctcatggcgg ccttcgtggc ccgccagcac
     2101 acggcgaaga cgccgctgtt cccgctgcgg atactgcgct cgcgcaacgt gaccggggcc
     2161 aacgtggtcc aggtgctgat ggtggccggc ctgttcggga tgttcttcct gggcgcgctg
     2221 tacctccagg gcatccaggg ctacagcccg atcgaggtgg gcctggcgta cctgccggtg
     2281 gcgctcctga tcggcggcgt gtcgctgaag ctggccgaca agctcatcct gcgcttcggc
     2341 gcccgccggg tgctgatcgc gggcctgacc tccgtgctgg gcggcctgct gctgttcacg
     2401 cgggcaccgc tggaggccac gtacgtgacc gacatcctgc ccacgatggt gctgttcggc
     2461 atcggcgccg gcctgtcgtt cccggccatg gtctcgctgg ccatgtcggg ggccacgcag
     2521 gaggacgccg gcctggcctc gggcctggtg aacaccaccc agcaggtggg cggcgcgctg
     2581 ggcctggccg tgctggccac gctctccacc acccgcaccg aggacaagct ggcggacggg
     2641 gcctcgcagg cctcggcgct ggtcgacggc taccagctgg cgttcttcgt cggcgccggc
     2701 ttcgccgccg cggccatcgc gttcgccgtc acggtgctgg agcccacccc cgagctcgag
     2761 atcgaggagc tgcccgacgt ggagctcgag gagggcgtcg aagtcggata ccgagaggct
     2821 gcctgaggcg aagctgtaaa gctggcggca gtgtccgccg gtgcgctcga cctccagcag
     2881 atcctccagc gcctacgcgc gagcgagtgg gtcgacctga cccactcgtt cgcgccgggc
     2941 gttccgcact accaccagtt cccggacgag cagcgcgagc agctgttcga ctactccgac
     3001 gggttccggg tgcacgagta ccgccacgtg ggccagtggg gcacgcacgt ggacccgccg
     3061 tcgcacttca tcgagggcgg gcgcaccctg gaccagctgc cggtcaccga catggtgctg
     3121 gagctggtgg tgctcgacgc gcgctcgcgc gtggaggccg accccgacta cgccgccgac
     3181 gcggcgctgg tgcgcgcgca cgaggaggag cacggccccg tgcccgaggg cgcgttcgtg
     3241 gcgctgcgca cgggctggtc ggagcgctgg cccgacccgg aggccttcga caacggcggc
     3301 cggcggccgg gctgggacgt cagcgcgctc gaggtcctgg tcgacgggcg cgggatcgcc
     3361 gccatcggcc acgagaccac cgacacggac ccggcggcgg tgctggccgc gggcagcacg
     3421 gcggccgaga cgctcatcct cgaccgcgac cggtggcaga tcgagggcat ggcctcgctg
     3481 gaccggctgc ccgcccgtgg cgccctgatc gtggccgcct ggcccaagcc cgccgggggc
     3541 tcgggcttcc ccgcgcgctg tttcgccatc ctgccctcat gagcgatctg cccgactccg
     3601 cccgctgcgt ggtcatcggc gg
//
LOCUS       DASUPW010000143         3080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_171913, whole genome shotgun sequence.
ACCESSION   DASUPW010000143 DASUPW010000000
VERSION     DASUPW010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171913"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VFY44_03220"
     CDS             <1..616
                     /locus_tag="VFY44_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909478.1"
                     /translation="APVRVASDGDQTAAAGPGPATGGGQSAGDSTGSLQVGAPVADLT
                     TRALSEGDDEAGAPSGQDAAVQGSTGSVQVGGPRVRSALRVASAGDSRTVPTATESVV
                     FAGPAPTVGIDEGSTGRADRGRGSRPAPNAGGAFPENAGGNAPGATSGPGSLVPAAYV
                     AATGAAGELPRTGLALMWLLGLGLALTAGGAALRRSAFARGAAA"
     gene            713..868
                     /locus_tag="VFY44_03225"
     CDS             713..868
                     /locus_tag="VFY44_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909479.1"
                     /translation="MLTATAAAGTAGGLRAWLATRAWLSPLALRRLTVALLALAVAAS
                     AIGLQSA"
     gene            complement(865..1995)
                     /locus_tag="VFY44_03230"
     CDS             complement(865..1995)
                     /locus_tag="VFY44_03230"
                     /inference="COORDINATES: protein motif:HMM:NF019522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEX5909480.1"
                     /translation="MLVAAVAFLALATVVGLVALWPDGGRPETAGGPAKTNAVDAKVV
                     TVQTVDCRGPQRQDCRRAVIEVDGRRSPITFGPVESAPDVEAGEDIRVAKVDAPPGAV
                     GSEPYSFVGRDRRGALIWLTAIFAAIVVALTRWRGVLALGGFALSLGLVTQFIVPAIA
                     QGESPMGVAIVGSFAVMFVTVGLTYGLTAQSAAAALGIAGSLLFAAVLGSVSVDAVKL
                     DGYGSEFSSVLSQSGSDVSLQGIVIAGLVIGALGVLADMAVTQASAVMALRRANPAMT
                     ARELFAAGFGVGRDHLVATTHTLVLAYVGAALPLLLVLYAVDLGTVDAINSEDVAEPI
                     VATLVGAISLLISVPVTTALATLVVAHVPADTVPEDHHGHAH"
     gene            complement(2090..2162)
                     /locus_tag="VFY44_03235"
     tRNA            complement(2090..2162)
                     /locus_tag="VFY44_03235"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2127..2129),aa:Thr,seq:ggt)
     gene            complement(2329..2410)
                     /locus_tag="VFY44_03240"
     tRNA            complement(2329..2410)
                     /locus_tag="VFY44_03240"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2374..2376),aa:Tyr,seq:gta)
     gene            2499..>3080
                     /locus_tag="VFY44_03245"
     CDS             2499..>3080
                     /locus_tag="VFY44_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909481.1"
                     /translation="MATVPAPYLKKALEGAEAHPAETEPVTSGMFTAILLAYIEKRGG
                     RAAVERTLELAGLLGRERDLRDESSWWTFVQKQALIHAAADVLDDPQVTMRAGEYALD
                     AGVGDALKAALRAFGSPELVYANVARANHKFNRHSVMKVHSRGDGHVTVAFRHIVDVR
                     PTRLDCLFNVGVLSCGPTLFGMAPARVTHTECVL"
BASE COUNT          411 a         1126 c         1165 g          378 t
ORIGIN      
        1 cgccccggtg cgggtggcca gcgacggtga ccagacggcg gccgccggac ccggtccggc
       61 gacgggcggc ggtcagtcgg ccggcgattc caccggatcg ctccaggtgg gcgccccggt
      121 cgcggacctg accaccaggg ccctcagcga gggcgacgac gaggccggag ccccgagcgg
      181 ccaggacgcg gccgtccagg gttccacggg cagtgtgcag gtcggcggtc cccgtgtcag
      241 gtccgccctg cgggtggcca gcgcgggtga ctcgcggacg gttcccaccg cgaccgagtc
      301 ggtcgtgttc gccgggccgg ccccgacggt cggcatcgac gaggggagca ccgggcgcgc
      361 ggaccgcggc cgtggctcca ggcctgcccc gaacgcgggc ggcgccttcc cggagaacgc
      421 cggcggcaac gcccccggcg cgacgtccgg gccgggatcg ctggtccccg cggcctacgt
      481 ggccgcgacg ggcgctgccg gcgagttgcc ccgcacgggc ctcgcgctga tgtggctgct
      541 gggcctgggg ctggcgctca cggccggcgg cgcggcgctg cggcgctcgg cgttcgcgcg
      601 tggtgcggcc gcgtgacggc ggatcggtcg ccggcgcgcg ggacagcgcc ggcggccggt
      661 cgccctctgc ggcgatgacg tccgacatac tgggcccgtg tgcgctcagt gcatgctcac
      721 ggcgaccgcc gccgccggca cagccggcgg gttgcgcgcc tggctggcga cgcgcgcgtg
      781 gctgtcgccg ctggccttgc gccggctgac ggtggcgctg ctggcgctgg ccgtggcggc
      841 cagcgcgatc ggcctgcaga gcgcctagtg ggcgtggccg tggtggtcct cgggcacggt
      901 gtccgccggc acgtgtgcga ccacgagggt ggccagcgcg gtggtcaccg ggaccgagat
      961 cagcagcgag atggcgccga ccagcgtggc cacgatcggc tccgccacgt cttccgagtt
     1021 gatggcgtcg acggtgccca gatcgaccgc gtagagcacc agcagcaggg gtagcgccgc
     1081 gccgacgtag gccaggacca gcgtgtgcgt ggtggccacc aggtggtccc gcccgacccc
     1141 gaacccggcc gcgaacagct cgcgcgcggt catggccggg ttcgcgcggc ggagggccat
     1201 gaccgcgctg gcctgcgtga cggccatgtc cgccagcacc ccgagcgcgc cgatgacgag
     1261 cccggcgatc acgattccct gcagcgacac gtcgctgccg gactgggaca ggacggacga
     1321 gaactcgctg ccgtagccgt cgagcttgac cgcgtccacg ctgacggacc cgagcacggc
     1381 ggcgaacagc aggctgccgg cgattcccag cgcggcggcg gcgctctggg cggtcaggcc
     1441 gtaggtcagg ccgaccgtca cgaacatcac cgcgaacgac ccgacgatgg ccacccccat
     1501 cggggactct ccctgcgcga tcgccggcac gatgaactgg gtgaccaggc ccaggctgag
     1561 cgcgaacccg cccagcgcca gcaccccgcg ccagcgggtc agggcgacca cgatggccgc
     1621 gaagatcgcg gtcagccaga tcagcgcgcc gcgccggtcg cggcccacga agctgtaggg
     1681 ctcggagccg accgcccccg gtggggcgtc gaccttggcg acccggatgt cctcaccggc
     1741 ctccacgtcc ggcgccgact cgaccgggcc gaacgtgatc ggggaccggc gcccgtcgac
     1801 ctcgatcacc gcacgccggc agtcctggcg ctgcgggccc cggcaatcca ccgtctgcac
     1861 ggtgaccacc ttcgcgtcga ccgcgttcgt cttcgccggg ccgccggcgg tctcgggccg
     1921 gcccccatcc ggccacagcg ccaccaggcc gaccacggtg gccagggcca ggaaggccac
     1981 cgcggcgacg agcatgcggc cccagccgac agagagaagt tgcacgcgct ccccttcgct
     2041 ggggcggcgg cggcctcctg cgtcggcggc cgcgcgggag gttcgcgaga gccctcgtcc
     2101 ggactcgaac cggagacccc ttccttacca tggaagtgct ctaccaactg agctacgagg
     2161 gcaggtcggg agagcctagc gagccttcgg ccaggggcgc atctgcgcgc ccacgcggac
     2221 ttgcggacct cgtgggtcgc cagacgcgca tcggcgcgca cgtggggacc cgttcggccc
     2281 ggacccgcca tgggtcggca cgtgcgcgat gcgcggcccc taggccgatg gtgggggcag
     2341 gattcgaacc tgcgaaggca gagccaacgc gtttacagcg cgtcccgttt gaccgcttcg
     2401 gtaccccacc ggggcgagcg gggagtctag agaggccgtc ctgcgtccgt ggtgcgccac
     2461 gcctcaagtg aagccccccg gacgccgatg ctccctcgat ggccacggtc ccggctccct
     2521 acctgaagaa ggccctggag ggcgctgaag cgcatcccgc cgagaccgag ccggtgacct
     2581 cggggatgtt caccgcgatc ctgctggcgt acatcgagaa gcgaggcggc cgggcggcgg
     2641 tcgagcgcac gctggagctg gccgggctgc tgggccgcga gcgcgacctc cgcgacgagt
     2701 cgagctggtg gacgttcgtg cagaagcagg cgctgatcca cgccgcggcc gacgtgctgg
     2761 acgaccccca ggtgaccatg cgcgcgggcg agtacgcgct cgacgccggc gtgggcgacg
     2821 cgctgaaggc ggcgctgcgg gcgttcggct cgccggagct ggtgtacgcg aacgtggccc
     2881 gcgccaacca caagttcaac cgccactcgg tgatgaaggt gcactcgcgc ggggacgggc
     2941 acgtgaccgt ggccttccgc cacatcgtcg acgtgcgccc gacccggctg gactgcctgt
     3001 tcaacgtggg cgtgctgagc tgcgggccga cgctgttcgg gatggccccc gcgcgcgtga
     3061 cccacaccga gtgcgtcctg
//
LOCUS       DASUPW010000144         5783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1279782, whole genome shotgun sequence.
ACCESSION   DASUPW010000144 DASUPW010000000
VERSION     DASUPW010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279782"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VFY44_03250"
     CDS             <1..406
                     /locus_tag="VFY44_03250"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX5909482.1"
                     /translation="RAGLANQPQSFAIDDGLELTGAFVAAAVRCAPPANKPTPAERDR
                     CAPYAERELELIGPRVILCLGAFAWDAALRLTGPPPKPKPKFGHGAEHAVPGGPVMLG
                     CFHVSQQNTFTGKLTEPMIDAVFERAKELAAR"
     gene            complement(392..1081)
                     /locus_tag="VFY44_03255"
     CDS             complement(392..1081)
                     /locus_tag="VFY44_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX5909483.1"
                     /translation="MHASIRTGLVLILLAAGCGGDAEEPRLGDARSTPGTTLVASLGD
                     SITAGSPAWDPDEAVRRQAGASDPRSQYEYWAERRLRGVRFRNCGVFGERTDQIARRL
                     DRCARGAKVLIVQGGINDIAQGRGVEEAARDLRAMARRGRELGLRVALVEVLPWDNGY
                     PRADPLIRALNRRIGAIGRDLRVPVYPWYDRLESPGRPGRMGPGLTADGDHPSVDGYR
                     RLAADVRLPRR"
     gene            1180..1599
                     /locus_tag="VFY44_03260"
     CDS             1180..1599
                     /locus_tag="VFY44_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909484.1"
                     /translation="MNRFTRADDEGPRTVRALLERPPSVQEKGWSEAVLELFAADPRL
                     EWVPVVGSGNRALAIAGRPLGMNVEPRLAGIEQVRPDETLPEASRRALARPASERLVP
                     LIRCDSRERYVAVVRIERIIEELADAYEAQPLPSPQS"
     regulatory      1598..1754
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            1764..2555
                     /locus_tag="VFY44_03265"
     CDS             1764..2555
                     /locus_tag="VFY44_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEX5909485.1"
                     /translation="MSRRLTPRNVLLTGALLALPLSAPAVATAQLGDETLRKGDSGAD
                     VKELQRELRAAGYRTAVDGEYGNDTVRRVRAFEGNEQLAVDGKVTPADAKKLQEVTER
                     GDNGEAELPTGGMAPGETEKKQTTPGAKATVGPDGFAVAPAGAPPEVQKAIAAGNEIA
                     KTPYKYGGGHAKLKDSGYDCSGSISYALRKAGIMKSSLASGPMMKFGESGPGEWITLY
                     ANSGHAYMVIAGLRFDTSARKQEGSRWSDEARSSKGYTARHPEGF"
     gene            2595..3992
                     /locus_tag="VFY44_03270"
     CDS             2595..3992
                     /locus_tag="VFY44_03270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-efflux pump"
                     /protein_id="HEX5909486.1"
                     /translation="MFSTGKGTQQRTALISVGAAIVLIAVKLGTGLATGSLAFLAEAA
                     HSATDLVAALLTLFAVRVAVRPADDDHQYGHGKAQHLAALGESAFLVLVSLVIGFLAA
                     QRLAEGGGHEIEVAWWAIAVLGLVITVDASRMVVSHRASRRHGSAALAANALHFGSDL
                     AGSVAVLVGLVFVSAGYESADAVAALVVAVLVIAAAVRLARQSIDVLMDRSTAESTAA
                     VEAALAAVDDRFVVRRIRARHAGDSHFVDLVVGIPPDAHISQAHATADDIEAAVERRL
                     PNTDVMVHVEPIEGEGDLRERATVAALSVAEVREVHNVRAMRTDGTYELTLHAKVPGG
                     QSLEEAHDTADRVESSIHRALPELARVHTHIEPLAETDEAVRPQSDKVTADRQAITDL
                     ALRHTGAEPLDVSFRDSAGGRIALVTVAVPGQKSLREAHRIAGRIEEDLRRERPDLAD
                     AVVHTEPAREEHPTG"
     gene            4005..>5783
                     /locus_tag="VFY44_03275"
     CDS             4005..>5783
                     /locus_tag="VFY44_03275"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX5909487.1"
                     /translation="MAGRLVIAVMAGLLALCAAVASADAATPPKPRALAGKATDSVGA
                     VVRRTKYGVPHVTARTIEGAAYGQAYAFAEDNLCTLADDMVTVNAERSRWFGPDASYT
                     VSGNQQKYGNLNSDFFYERINATGKIEALLAKKPPQGPLPELRQATRGFVRGYNAYLA
                     DVGGAKGLSDPRCKGEPWVRPITEQDLYRRYFQLGELVSGLIALDGIANAAPVGATSS
                     STRSGMVRELGRRKTGLGVGSNAWGLGGDATDNGKGAVVGNPHFPWAGSERFYQSHVT
                     VPGQIDVSGGTLYGVPAVLIGHTKGLAWSHTVATAWRLTPFELKLVPGDPYSYFLDGR
                     PTKMKATKLTVEVKGGQKRTRTVYETVHGPMFTSLLGLPLFPWTPATGYAMGDVNADN
                     FRYLNHFLETNKAQTVRQYDAIQRRYQGIPWVNSLAADSKGEAYFSMNGAIPNVPNEK
                     AQRCQTALGLVTFGQLGLPTLDGSRSDCEWGQAAGSADKGLLPNARTPSLFRRDYVAN
                     GNDSHWLINDKAPLEGFDRIVGIERAEVTPRTRLGLTMIRDRLAGRDGLPGNRFNQRN
                     LEWVALGNRQYLGELWRGELVRICRAD"
BASE COUNT          858 a         2135 c         2082 g          708 t
ORIGIN      
        1 ccgcgccggg ctggccaacc agccccagtc gttcgccatc gacgacgggc tggagctgac
       61 cggcgcgttc gtggccgcgg ccgtccgctg cgccccgccc gccaacaagc ccaccccggc
      121 cgagcgcgac cgctgcgcgc cctacgccga gcgggagctg gagctgatcg gcccccgggt
      181 gatcctgtgc ctgggcgcgt tcgcgtggga tgccgccctg cggctgaccg gcccgccccc
      241 caagcccaag ccgaagttcg gccacggggc cgagcacgcc gtgcccggtg gcccggtcat
      301 gctgggctgc ttccacgtca gccaacagaa cacgttcacg ggcaagctga ccgagccgat
      361 gatcgacgcc gtgttcgagc gcgcgaagga gctagcggcg cggtaggcgc acgtcggcgg
      421 ccagccgcct gtagccgtcg acggaggggt ggtcgccgtc cgcggtcagg ccggggccca
      481 tgcgaccggg ccggccgggg ctctcgagcc ggtcgtacca cgggtacacg ggcacgcgca
      541 ggtcgcggcc gatcgccccg atgcggcggt tgagcgccct gatcagcggg tccgcgcgcg
      601 ggtagccgtt gtcccagggc aggacctcca ccagcgccac ccgcagcccg agctcgcgcc
      661 cgcggcgggc catggcgcgc aggtcgcgcg ccgcctcctc gacccccctg ccctgggcga
      721 tgtcgttgat cccgccctgc acgatcagca ccttggcccc ccgggcgcac cggtccagcc
      781 ggcgcgcgat ctggtcggtg cgctcgccga acacgccgca gttgcggaag cgcacgcccc
      841 gcaggcgccg ctcggcccag tactcgtact ggctgcgcgg gtccgacgcg ccggcctgcc
      901 ggcgcaccgc ctcgtccgga tcccacgcgg gcgaaccggc ggtgatcgag tcgcccagcg
      961 aggccaccag ggtggtcccg ggggtgctcc gcgcgtcgcc gaggcgcggc tcctcggcat
     1021 cgccgccgca gcccgccgca agcaggatca ggaccaggcc tgtacgaatg gaggcgtgca
     1081 cgtcaggttc tcaatacgca ggccgtgcaa agtcggatcc gcgctggcga gggtgcgcca
     1141 ccgcacacag gctcgtagca attcgagcaa ggacgcttga tgaacaggtt caccagggca
     1201 gatgacgagg ggccacggac ggtccgggcg ctcctggagc ggccaccctc cgtgcaggag
     1261 aagggctgga gcgaggcggt gctggagctg ttcgccgccg acccccggct tgagtgggtg
     1321 cccgtggtgg ggtcgggcaa ccgcgcgctg gccatcgcgg gacggcccct gggcatgaac
     1381 gtggagccgc gcctggccgg gatcgagcag gtacggcccg acgagacgct cccggaggcc
     1441 agccggcggg cgctcgcgcg acccgcatcc gagcgcctcg tgccgctgat ccggtgcgac
     1501 agccgggagc gctacgtggc cgtggtgcgc atcgagcgga tcatcgaaga gctggctgac
     1561 gcatacgagg cgcagccgct accctccccg cagtcgtaag caaacccagc cgagtccgcg
     1621 cccgcccgac ggccgcggaa cgggggaccc accgggccac cggccctggg gcgaatcgcc
     1681 gtcgtagagc ggcgtagcgc acttttcagc gcgagcccgt cagctaaccc cgtaggcacg
     1741 agaagagagg gccgtatcaa cagatgtctc gccgcctgac cccacgtaac gtgctgctga
     1801 ccggcgcgct gctcgcgctg ccactgtccg cgcccgccgt cgcgaccgcc cagctgggcg
     1861 acgagaccct gcgcaagggc gactccggcg ccgacgtgaa ggagctccag cgtgagctgc
     1921 gggcggccgg ataccgcacc gcggtcgacg gcgagtacgg caacgacacc gtcaggcgcg
     1981 tgcgcgcctt cgagggcaac gagcagctgg ccgtggacgg caaggtcacg ccggccgacg
     2041 ccaagaagct ccaggaggtc accgagcgcg gcgacaacgg cgaggccgag ctgcccaccg
     2101 gcggcatggc cccgggcgag accgagaaga agcagaccac cccgggcgcc aaggcgaccg
     2161 tggggccgga cggcttcgcc gtcgcccccg ccggcgctcc gccggaggtc cagaaggcca
     2221 tcgccgccgg caacgagatc gccaagactc cgtacaagta cggcggcggc cacgccaagc
     2281 tcaaggacag cggctacgac tgctcgggct ccatctccta cgcgctgcgc aaggccggga
     2341 tcatgaagtc ctcgctggcc tccggcccga tgatgaagtt cggcgagagc ggccccggcg
     2401 agtggatcac cctctacgcc aactccggcc acgcctacat ggtcatcgcc ggccttcggt
     2461 tcgacaccag cgcgcgcaag caggagggct cgcgctggtc ggacgaggcg cgctccagca
     2521 agggatacac cgcgcgccac cccgaagggt tctaagcctc agaatgtgag gagggccggg
     2581 cgggccctcc tcacatgttc tccaccggca aaggcaccca gcagcgcacg gcgctgatca
     2641 gcgtcggcgc cgcgatcgtc ctgatcgcgg tcaagctggg cacgggcctg gccaccggct
     2701 cgctggcctt cctggccgag gccgcccact cggccaccga cctggtggcc gcgctgctca
     2761 ccctgttcgc cgtgcgcgtg gccgtccgcc ccgcggacga cgaccaccag tacggccacg
     2821 gcaaggccca gcacctggcg gcgctgggcg agagcgcgtt cctggtgctg gtgtcgctgg
     2881 tcatcggctt cctggcggcg cagcggctgg ccgagggcgg cggccacgag atcgaggtgg
     2941 cctggtgggc catcgccgtc ctggggctgg tgatcaccgt ggacgcatcg cgcatggtgg
     3001 tcagccaccg ggcctcgcgc cgccacggct ccgccgcgct ggccgcgaac gcgctgcact
     3061 tcggttccga cctggcgggc tcggtggccg tgctggtcgg gctcgtcttc gtgtcggcgg
     3121 gctacgagtc cgccgacgcc gtggccgcgc tggtggtggc ggtgctggtc atcgccgccg
     3181 cggtgcgcct ggcgcgacag tcgatcgacg tgctgatgga ccgcagcacc gccgagtcca
     3241 ccgccgcggt ggaggccgcg ctggcggcgg tcgacgaccg cttcgtggtc cggcgcatcc
     3301 gcgcccgcca cgcgggtgac agccacttcg tcgacctggt cgtgggcatc ccgcccgacg
     3361 cccacatctc gcaggcgcac gcgaccgccg acgacatcga ggccgcggtc gagcgccggc
     3421 tgcccaacac cgacgtgatg gtgcacgtgg agccgatcga gggcgagggg gacctgcgcg
     3481 agcgcgcgac cgtggccgcg ctgtcggtgg ccgaggtgcg cgaggtgcac aacgtgcgcg
     3541 cgatgcgcac cgacggcacc tacgagctga cgctgcacgc caaggtgccc ggtggccagt
     3601 cgctggagga ggcccacgac acggccgacc gtgtggagag ctcgatccac cgggcgctgc
     3661 ccgagctggc ccgcgtgcac acccacatcg agccgctggc ggagaccgac gaggccgtgc
     3721 gcccccagag cgacaaggtg accgccgacc gccaggccat caccgacctg gcgcttcgcc
     3781 acaccggagc cgagccgctg gacgtgagct tccgcgactc ggccggcggg cggatcgcgc
     3841 tggtcacggt cgcggtgccg ggccagaagt cgctgcgcga ggcgcaccgg atcgccggcc
     3901 ggatcgagga ggaccttcgc cgcgagcggc cggacctggc cgacgcggtg gtgcacaccg
     3961 agccggcgcg cgaggagcat cccacggggt aggatcgcgg cctcgtggct gggaggctcg
     4021 tgatcgcggt gatggccggg ctgctcgcgc tgtgcgcggc ggtcgcgtcc gccgacgccg
     4081 ccactccacc caagccgcgc gcgctggccg gcaaggccac cgacagcgtc ggcgccgtgg
     4141 tcaggcgcac caagtacggg gtgccccacg tgaccgcccg gaccatcgag ggcgccgcct
     4201 acgggcaggc ctacgcgttc gccgaggaca acctgtgcac cctggccgac gacatggtca
     4261 cggtgaacgc cgagcgctcg cggtggttcg gcccggacgc cagctacacc gtgtccggca
     4321 accagcagaa gtacgggaac ctgaactcgg acttcttcta cgagcgcatc aacgcgaccg
     4381 gcaagatcga ggcgctgctg gccaagaagc ctccgcaggg cccgctgccc gagctgcgcc
     4441 aggccacgcg cgggttcgtg cgcggctaca acgcctacct ggccgacgtg ggcggcgcca
     4501 agggcctgag cgacccgcgc tgcaagggcg agccgtgggt gcggccgatc accgagcagg
     4561 acctgtaccg gcgctacttc cagctgggcg agctggtgtc cgggctgatc gccctggacg
     4621 gcatcgccaa cgccgcgccg gtgggcgcca cgtcgtcgtc gacccggtcc gggatggtgc
     4681 gcgagctcgg ccggcgcaag accggcctgg gcgtgggctc caacgcctgg ggcctgggcg
     4741 gcgacgccac cgacaacggc aagggcgccg tggtgggcaa cccccacttc ccctgggccg
     4801 gctccgagcg cttctaccag tcgcacgtga ccgtgcccgg tcagatcgac gtctccggcg
     4861 gcacgctcta cggcgtcccg gcggtgctga tcgggcacac gaagggcctg gcctggtcgc
     4921 acaccgtggc caccgcgtgg cggctgaccc cgttcgagct caagctcgtc cccggcgacc
     4981 cctacagcta cttcctggac ggccggccga ccaagatgaa ggccacgaag ctgacggtcg
     5041 aggtcaaggg cggccagaag cgcacgcgca ccgtctacga gaccgtgcac ggccccatgt
     5101 tcacctcgct gctgggcctt ccgctgttcc cgtggacgcc ggccaccggc tacgcgatgg
     5161 gcgacgtgaa cgcggacaac ttccgctacc tcaaccactt cctggagacc aacaaggcgc
     5221 agacggtgcg ccagtacgac gccatccagc gccgctacca gggcatcccg tgggtgaact
     5281 cgctggccgc ggacagcaag ggcgaggcct acttctcgat gaacggggcg atccccaacg
     5341 tgcccaacga gaaggcgcag cgatgccaga ccgcgctggg cctcgtgacc ttcggccagc
     5401 tcggcctgcc cacgctggac ggctcgcgct cggactgcga gtggggccag gcggcggggt
     5461 cggccgacaa gggcctgctg cccaacgccc ggacgccgtc gctgttccgc cgcgactacg
     5521 tggccaacgg caacgacagc cactggctga tcaacgacaa ggcgccgctg gagggcttcg
     5581 accggatcgt gggcatcgag cgcgccgagg tgaccccgcg cacgcgcctg ggcctgacca
     5641 tgatccgcga ccgcctggcc ggccgcgacg gcctgcccgg caaccgcttc aaccagcgca
     5701 acctggagtg ggtcgcgctg ggcaaccgcc agtacctcgg cgagctctgg cgcggcgagc
     5761 tggtgcgcat ctgccgcgcc gac
//
LOCUS       DASUPW010000145         2195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_172704, whole genome shotgun sequence.
ACCESSION   DASUPW010000145 DASUPW010000000
VERSION     DASUPW010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172704"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VFY44_03280"
     CDS             complement(<1..275)
                     /locus_tag="VFY44_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909488.1"
                     /translation="MSVQRTLEERRDDLQALEGVLDLGDRRVQAVHHAHVGQARDGLD
                     GAVADALAEADARAALGRLGQPGDLSPEVVTALLQGSLHGHAHELRH"
     gene            304..1803
                     /locus_tag="VFY44_03285"
     CDS             304..1803
                     /locus_tag="VFY44_03285"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX5909489.1"
                     /translation="MTAPATLTHWIGGAPDPAPADRHTDVTESATGRVIARVPMAGTD
                     VVDRAVGAARAGADEWGQASIAARTKVMFAFRELVHAHREDLAALIVREHGKVFDDAL
                     GEVQRGLEVAEFACGVGELLKGAMSAQISRGVDSYSLPQPVGVAAGITPFNFPVMVPM
                     WMFPVALACGNGFVLKPSEQDPSASLMLADLLARAGLPEGAFNVVHGDRDAVDALLTH
                     PGVDSVSFVGSTPIARHIYETATAHGKRVQALGGAKNHAVVLPDADLDVAADALVSAG
                     YGSAGQRCMAVSVAVAVGDVAEPLIAKVRERMAALTVGDGADEGSDMGPLVSARHLEK
                     VRGLVDAGVQEGAELLEDGRGIDVGGDGHFIGPCLFDHVRPGMSIYDQEIFGPVLLVV
                     RCDTYPEALELVNANPHGNGAAVFTNDGGAARLFEQQVTAGMVGINVPIPVPMAYHSF
                     GGWKASLFGDLHVHGRHGVRFYTRDKVVTRRWVDPAGRRGVDLGFPTSD"
     gene            1868..>2195
                     /locus_tag="VFY44_03290"
     CDS             1868..>2195
                     /locus_tag="VFY44_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909490.1"
                     /translation="MARHITRRQALAGSAGLALAPALTAPGAALARRDATDHFAVDVP
                     LPKAGAARAGWTTSKPVATGRSFDLIGARWRSGRGVAVQVRVKKHGRWSRWLDLPNAL
                     DHGPDRG"
BASE COUNT          279 a          787 c          828 g          301 t
ORIGIN      
        1 tcatgcctaa gctcgtgtgc atgtccgtgc aacgaaccct ggaggagcgc cgtgacgacc
       61 tccgggctca ggtcgcccgg ctgaccaagg cgcccgagcg cggctcgggc atcagcttcg
      121 gcaagcgcgt cggcgacggc accatcgagg ccgtcgcgcg cctgtccgac gtgagcgtgg
      181 tggacagcct gaacgcgtcg atcgcccagg tcgagcacgc cctcgagcgc ctggaggtcg
      241 tcgcggcgct cctccagggt tcgttgcacg gacatgcaca cgagcttagg catgatggac
      301 gtcgtgaccg ctcccgcgac cctgacccac tggatcggcg gtgcgcccga cccggccccc
      361 gccgaccgcc acaccgacgt cacggagtcg gccaccggcc gcgtgatcgc gcgggtgccg
      421 atggccggta ccgacgtggt cgaccgcgcc gtgggcgccg cccgcgcggg ggcggacgag
      481 tggggccagg cctcgatcgc cgcccggacc aaggtcatgt tcgccttccg cgagctggtg
      541 cacgcccacc gcgaggacct ggcggcgctg atcgtccgcg agcacggcaa ggtcttcgac
      601 gacgcgctcg gcgaggtgca gcgcggcctg gaggtcgcgg agttcgcctg cggcgtcggc
      661 gagctgctca agggcgccat gtcggcgcag atctcgcgcg gggtcgacag ctactcgctg
      721 ccccagccgg tgggcgtggc cgcgggcatc acgccgttca acttcccggt gatggtgccc
      781 atgtggatgt tcccggtggc gctggcgtgc ggcaacgggt tcgtgctcaa gccgtccgag
      841 caggatccct cggcgtcgct gatgctggcg gacctgctgg cccgggccgg cctgcccgag
      901 ggcgccttca acgtcgtgca cggcgaccgc gacgcggtcg acgcgctgct gacccacccg
      961 ggcgtggact ccgtgtcgtt cgtgggctcg acgcccatcg cccgccacat ctacgagacc
     1021 gcaaccgcgc acggcaagcg cgtgcaggcc ctgggcggcg cgaagaacca cgccgtggtg
     1081 ctgcccgacg ccgacctcga cgtggccgcc gacgcgctgg tctcggcggg ctacggctcc
     1141 gccggccagc gctgcatggc ggtgtcggtc gcggtggccg tgggcgacgt ggccgagccg
     1201 ctgatcgcga aggtgcgcga gcgcatggcg gcgctgacgg tgggcgacgg cgccgacgag
     1261 ggatcggaca tgggtccgct ggtcagcgcc cgccacctgg agaaggtgcg cggcctggtc
     1321 gacgcgggcg tgcaggaggg ggcggagctg ctggaggacg ggcgcgggat cgacgtcggc
     1381 ggcgacgggc acttcatcgg cccgtgcctg ttcgaccacg tgcgccccgg catgagcatc
     1441 tacgaccagg agatcttcgg cccggtcctg ctggtggtgc gctgcgacac ctacccggag
     1501 gccctggagc tggtgaacgc caacccgcac ggcaacggcg cggcggtgtt caccaacgac
     1561 ggcggcgccg cgcggctgtt cgagcagcag gtgaccgccg gcatggtggg catcaacgtg
     1621 ccgatcccgg tgccgatggc ctaccactcg ttcggcggct ggaaggcgtc gctgttcggc
     1681 gacctgcacg tgcacggccg ccacggggtc cgcttctaca cccgcgacaa ggtcgtgacc
     1741 cggcgctggg tcgacccggc gggccggcgc ggcgtggacc tcggctttcc cacgtccgac
     1801 tgacgcccgg caagatctct tgcgtggcac caccgcatcg gggggtggtt gtcagggttg
     1861 agtgcgtatg gcccgacaca tcacgcggcg tcaggcgctg gccggcagcg ccggcctcgc
     1921 gctggccccc gcgctcaccg cgcccggggc cgcgcttgcc cgccgtgacg ccaccgacca
     1981 cttcgccgtg gacgtgccgc tgcccaaggc gggcgcggcc cgggccggct ggaccacctc
     2041 caagccggtg gcgaccgggc gctcgttcga cctgatcggc gcccgctggc gctcgggccg
     2101 cggcgtggcg gtccaggtgc gcgtgaagaa gcacgggcgc tggagccgct ggctggacct
     2161 gccgaacgcg ctggaccacg gccccgaccg gggcg
//
LOCUS       DASUPW010000146         2814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1973890, whole genome shotgun sequence.
ACCESSION   DASUPW010000146 DASUPW010000000
VERSION     DASUPW010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1973890"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="VFY44_03295"
     CDS             complement(<1..1025)
                     /locus_tag="VFY44_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909491.1"
                     /translation="MTHRDGSTLLIDGRATVDPHLDRSTEEPDMAAARGKHAPQSQPQ
                     NRRKPARKTSRGVVALTVVAIVFAIVPVIAPAVLSASDLYHWAQSHYGLGVVAELAAF
                     VVAALDALAVACILITLVCAMLDRSAGIFAWGAWVIAGISAMANYRFGTAPDAPADRF
                     WFFPLMSFLGPVVMHGAVKLIRSAVQEEGGRRARKTPTFGLARWLPIVGSPLDTYGAY
                     RTAQVMQIERADEAITAYHELCPDGSLGVVRAIRARDAAEAAHARDRAAWEEAQERAH
                     AEWEADRARRDADRAARATTPRGEPRARDAKNARSNRARSARQTARADWWTTARDDWY
                     RAEYAARL"
     gene            complement(1025..1615)
                     /locus_tag="VFY44_03300"
     CDS             complement(1025..1615)
                     /locus_tag="VFY44_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909492.1"
                     /translation="MSVALERGLYHLLLVPAIGIAGSWMTDWNWWTFVAIDTALSVAY
                     EVARVGVAGVTIGVKDMIPVLIMLRIGLPTPTRTGLIVAGILLAALAAEVGWYLLRRR
                     IHPAVHVGRHWRRPALALAAVLAAVMVGLFAAGMSLMAPQVGPLLALGVIPPAVAAYH
                     FQTPPRRGGSRTRSVPTVLTRVRDKTPQTALVGGVR"
     gene            complement(1633..1908)
                     /locus_tag="VFY44_03305"
     CDS             complement(1633..1908)
                     /locus_tag="VFY44_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909493.1"
                     /translation="MTISPIPGYTLDEAATAAAAERVRAANPHLLVRVARPGDFDHDT
                     VYEGGVLVLPVAVFQGPVVEMTWLGSGPRALSHSVVTAEGDVLAVTR"
     gene            complement(1905..2141)
                     /locus_tag="VFY44_03310"
     CDS             complement(1905..2141)
                     /locus_tag="VFY44_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909494.1"
                     /translation="MTTLALFRRPDRTPTQRLGSVTGYAMWAVAGILACYVTAQSVTP
                     LPFWPAYLLGAVAAVLAGTSVGCWLATRDRRYPS"
     gene            complement(2329..2595)
                     /locus_tag="VFY44_03315"
     CDS             complement(2329..2595)
                     /locus_tag="VFY44_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909495.1"
                     /translation="MITLASASNVNPRARAAWHIARRTLEVVSFLSFLSTGVGLCWAV
                     AAVYGLSEALGAAGWLQLAVALPLAAGVVDLVRRRTPNVRRYQP"
     gene            complement(2592..>2814)
                     /locus_tag="VFY44_03320"
     CDS             complement(2592..>2814)
                     /locus_tag="VFY44_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909496.1"
                     /translation="ALSCWDYVQRTGLLNECAPENRALIKAAYEDCYQTSGDATSDER
                     MPTVDLTRLCQLSAARTRAEASGYLRSAA"
BASE COUNT          421 a          930 c         1116 g          347 t
ORIGIN      
        1 aggcgcgcgg cgtactccgc gcggtaccag tcgtcgcgcg cggtggtcca ccagtccgcg
       61 cgcgcggtct ggcgcgcgct gcgcgcgcgg ttggagcgcg cgttcttcgc gtcgcgcgcg
      121 cgcggttcac cgcgcggtgt agtggcgcgc gccgcgcggt ctgcgtcgcg tcgcgcgcgg
      181 tcggcttccc actcggcgtg cgcgcgctcc tgcgcttcct cccacgccgc gcggtcgcgc
      241 gcgtgcgcgg cctcggcggc gtcgcgcgca cggatcgcgc ggaccacacc cagtgacccg
      301 tcggggcaca gttcgtggta ggcggtgatc gcctcatcgg cgcgctcgat ctgcatcacc
      361 tgcgcggtcc ggtacgcgcc gtaggtgtcc agcggggagc cgacgatcgg gagccagcgg
      421 gccagcccga acgtcggtgt cttgcgtgcc cgccggccgc cctcctcttg gaccgcggag
      481 cggatcagtt tgaccgcgcc gtgcatgacg accgggccga ggaagctcat gagggggaag
      541 aaccagaacc ggtcggccgg ggcgtcgggg gctgtcccga accggtagtt cgccatggcc
      601 gagatcccgg cgatgaccca cgcaccccag gcgaagatgc cggccgaccg gtcgagcatc
      661 gcgcacacga gcgtgatcag gatgcaggcg acggcgaggg cgtccagggc ggccaccacg
      721 aacgcggcca gttcggcgac cacgccgagg ccgtagtggg actgcgccca gtggtacagg
      781 tcggatgcgg acagcacggc cggggcgatc accgggacga tcgcgaacac gatcgccacc
      841 acggtgagcg cgaccacgcc tcggctggtc ttcctggccg gcttgcggcg gttctgcggc
      901 tgggactggg gcgcgtgctt tccacgtgcg gctgccatgt cgggctcctc ggtcgatcga
      961 tccagatgcg gatcaaccgt ggccctgcca tcgatgagga gggtggaccc gtcccggtgt
     1021 gtcatcagcg gacacccccg acgagggcgg tctgtggggt tttgtcccgg acccgggtga
     1081 gcacggtggg aaccgaccgg gtccgggacc ctccccgccg gggaggggtc tggaagtggt
     1141 acgccgccac agcgggcggg atcacgccga gggcgaggag cggcccgacc tgcggtgcca
     1201 tgagggacat cccggcggcg aacaggccga ccatgaccgc ggcgagcaca gcggcgaggg
     1261 cgagggcggg ccggcgccag tgccggccga cgtggacggc cgggtggatg cggcgtcgga
     1321 gcaggtacca gccgacctcg gcggccaggg cggcgagaag gatgccggcc acgatcagcc
     1381 cggtacgggt gggggtgggg agcccgatcc gcagcatgat caggacgggg atcatgtcct
     1441 tgacgccgat cgtgactccg gccacgccga cccgggccac ctcgtaggcg acggacaggg
     1501 cggtgtcgat ggcgacgaac gtccaccagt tccagtcggt catccaagat ccggcgatgc
     1561 cgatcgcggg aacgagcagc aggtggtaca ggccgcgctc cagcgcgacg gacacgacag
     1621 ccccgagcgc catcagcgag tcaccgccag gacgtcgccc tcagcggtga cgacggaatg
     1681 ggagagcgcc cgtgggccgc tgccgagcca cgtcatctcg acgacaggcc cctggaagac
     1741 ggcgacgggc aggaccagca cgccgccctc gtagacggtg tcgtggtcga agtcgccggg
     1801 acgggccacc cggaccagca ggtgcgggtt cgcggcgcgg acacgttcgg cggcggctgc
     1861 ggtggcggcc tcgtcgaggg tgtagccggg gattggtgag atggtcacga ggggtacctc
     1921 cggtcccggg tggccagcca gcagccgacc gacgtaccag ccagaacagc cgccacagcg
     1981 ccgagcaggt acgccggcca gaacggcagc ggggtgacgg actgggcggt gacgtagcag
     2041 gcgaggatcc cggcgaccgc ccacatggcg tagccggtca cgctgccgag ccgctgcgtg
     2101 ggtgtccggt cggggcgccg gaacagggcg agggtggtca tggggtgtac ctccgagggg
     2161 ttgagcggcg ctccagggcg accgcgcgga gagcgacgag cagccaggcg ccgacggtcg
     2221 cggcggccac ggtccaggcg ggccacgtga ccggctggcc ggtggtttcg gctaggacgg
     2281 cggcgcagac ggcgaggccg gcgacgcgga gccagaactc gtcgggggtc acggctggta
     2341 cctgcggacg ttcggggtgc ggcggcggac gaggtcgacc acgccggccg cgagcggcag
     2401 cgcgacggcc agttggagcc agccggccgc gccgagggct tcggacaggc cgtagaccgc
     2461 agccaccgcc cagcagaggc cgacgccggt cgacaggaag gacaggaacg agacgacctc
     2521 cagcgtgcgg cgggcgatgt gccacgccgc gcgggcacgg gggttgacgt tggaggcgga
     2581 agccagggtg atcatgctgc actcctgagg tagccggacg cctcagcgcg ggtccgggcg
     2641 gcggaaagtt ggcacagccg ggtcaggtcg accgtcggca tgcgctcatc ggacgttgcg
     2701 tcgcccgacg tctggtagca gtcctcgtag gcggccttga tcagcgcccg gttctcgggg
     2761 gcgcactcgt tgagcaggcc ggtccgctgg acgtagtccc agcaggaaag agcg
//
LOCUS       DASUPW010000147         5571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_588380, whole genome shotgun sequence.
ACCESSION   DASUPW010000147 DASUPW010000000
VERSION     DASUPW010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5571
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588380"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2469)
                     /gene="ppdK"
                     /locus_tag="VFY44_03325"
     CDS             complement(<1..2469)
                     /gene="ppdK"
                     /locus_tag="VFY44_03325"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX5909497.1"
                     /translation="MNADTQLVYDFAEGSRDMRDLLGGKGANVAEMTRVLGPERVPAG
                     FTITTEACVAYMEADRTEPEGLEEQVDAALERLEEHAGKQLGDDEDPLLVSVRSGARE
                     SMPGMLDTVLNLGMNDTSVEGLARATDSERFAWDSYRRFVQMFANVCRGVSGEAIEKA
                     IKKRKSDAGVELDTELEVDDLKGLVDDFKDLYRDETGEDFPQDPRDQLAQAIRAVFDS
                     WVGPRAKEYRRINRIPDEWGTAVNVQQMVFGNKGDSSCSGVAFSRDEITGAPEPSGDY
                     LVNAQGEDVVSGVRTPKDISEMKESMPDAHGELLEILQTLEKHYGDMQDTEFTVEEGQ
                     LFMLQTRSAKRPAQAAVRFAVDAEKEGLLDKGEALATIEPDRLDALLHPTFAPDAEYE
                     VVAKGVSASPGAAKGEVVFTADAAIAAEKDGRDVILVRPFTEADDVGGFHAAKGILTA
                     EGGKASHAALVARGMGKPCVAGAGDLEIDLDAKTVKAGEHTLEEGDLIAIDGTKGQVT
                     TDDVELVDPEVDQNFETVLGWADELRRLGVRTNADTPEDAKRAREFGAEGIGLCRTEH
                     MFMAADRQPKMRAVIVADSDEERKEALAELLPLQQEDFEGLFEEMQGLPVTIRLLDPP
                     LHEFLPVPHELEEGSSERRRAEELQETNPMLGTRGVRLAILHPEISEMQVAAMVRAAK
                     AVKDRCGTAPVLEIMVPLVAYEKELAFMREVIDRAVQDNDGGDLELKVGTMLELPRAC
                     FLADVIGEEADFFSFGTNDLTQTALGFSRDDAEGKFLDRYLEQKIIDRSPFETLDAPG
                     VGQLVRMGAWLGRSRNKELGLGICG"
     gene            2487..3062
                     /locus_tag="VFY44_03330"
     CDS             2487..3062
                     /locus_tag="VFY44_03330"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5909498.1"
                     /translation="MPVSEQIILARHGETEWSRSGRHTGRTDIPLTEEGRRQAEALRP
                     ALADLEPALVLTSPLSRAAETCRLAGLGDVAVEEPALMEWDYGDYEGLTSDEIHENRP
                     GWNVFTDGAAGGETPDQVAARVDGVIERMAGAGGLCVAFAHGHVLRVLGARWVELPPA
                     AGARLGLSTATLSVLGTEHGVRALARWNAAP"
     gene            complement(3223..3627)
                     /locus_tag="VFY44_03335"
     CDS             complement(3223..3627)
                     /locus_tag="VFY44_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909499.1"
                     /translation="MQPWLKAMGHAKGPMPDAWIEDGRAGALRRTGFPRRPRMEPGDR
                     LVYYASVWRVVFAVVEVVGDPTDADPVASNPRRWPWTVPVEPLLAVPLLSNAPPVEAI
                     GVAARSMSQQSHIRLRDEHFERALEALAGVGR"
     gene            complement(3630..4346)
                     /gene="recO"
                     /locus_tag="VFY44_03340"
     CDS             complement(3630..4346)
                     /gene="recO"
                     /locus_tag="VFY44_03340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX5909500.1"
                     /translation="MPGSFKTEAVVLRSIRYGEADRVLHLYSPEHGRVGAVAKGVRRT
                     KSRLGGRLEPMARVKLVLHRGRGELCTISAVDTVHAHAGAREHWTALKHATQACEAVL
                     KLFDSTDPNPAAYNLLCNKLALLHADPEGASQERMLAFRMKLLLAAGFAPELGSCAAC
                     GEGEGLGAFSPAAGGVVCAACEAGSFPLDAAAHEYMVGAVGRPLAQAPAAEPRALAQA
                     DRAIAETLEHHAHVRLRRVA"
     gene            4365..4625
                     /locus_tag="VFY44_03345"
     CDS             4365..4625
                     /locus_tag="VFY44_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HEX5909501.1"
                     /translation="MPDITMYTTTPCGFCSQAKALLDARGFEYDVVDMSRDPEAREEL
                     VARTGRMTFPQIMVGDRTVGGFQELLAAHRAGTLSEVLADAA"
     gene            4714..>5571
                     /locus_tag="VFY44_03350"
     CDS             4714..>5571
                     /locus_tag="VFY44_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909502.1"
                     /translation="MAAALLLTALCLAGRGDAQPPPTPEFTDWTTATDTKVDGTLQGA
                     AMSLTGEIGPSTVTNGSETGFNNSAFTPPLATSDSLQVRTATAGNPYTFVFTQPVTNP
                     VIHIGDLSGTVSFNPSVRLSRVSGDAGFTVSGSTVTGTGGANGTVVPIDTYTGLAFTA
                     TSNPADVFYLQLGAFPGAVGRPAPPPDFRVQRGSGGQVVVAPGASTRVPVEVARNSPS
                     SGPINLSSLAQGTGVTTRMEPTQVNGRDLERATLVVMAAKSAKPGIRTVTVRGSPGAA
                     GAGPTSRTVQ"
BASE COUNT          740 a         2112 c         1926 g          793 t
ORIGIN      
        1 gccgcagatg cccaggccca gctccttgtt gcgcgagcgc ccgagccatg cccccatgcg
       61 caccagctgc cccacccccg gcgcgtccag ggtctcgaac gggctgcggt cgatgatctt
      121 ctgctccagg tagcggtcca ggaacttgcc ctcggcgtcg tcgcgggaga accccagcgc
      181 cgtctgggtc aggtcgttgg tgccgaacga gaagaagtcg gcctcctcgc cgatcacgtc
      241 ggccaggaag cacgcgcgcg gcagctcgag catggtgccg accttcagct ccaggtcgcc
      301 gccgtcgttg tcctgcaccg cgcggtcgat gacctcgcgc atgaaggcca gctccttctc
      361 gtaggccacc agcggcacca tgatctccag caccggcgcg gtgccgcacc ggtccttgac
      421 cgccttggcg gcccggacca tggccgccac ctgcatctcg ctgatctcgg ggtgcaggat
      481 ggccaggcgc acgccgcggg tgcccagcat tgggttcgtc tcctggagct cctcggcccg
      541 ccggcgctcg gacgagccct cctccagctc gtgcggcacg ggcaggaact catggagcgg
      601 cgggtccagc agccggatgg tcaccggcag cccctgcatc tcctcgaaca ggccctcgaa
      661 gtcctcctgc tggagcggga gcagctcggc cagcgcctcc tttcgctcct cgtcggagtc
      721 ggccacgatc accgcacgca tcttgggctg gcggtccgcg gccatgaaca tgtgctcggt
      781 ccggcacagg ccgatgccct cggcgccgaa ctcgcgtgcg cgcttcgcgt cctcgggggt
      841 gtccgcgttg gtgcgcacgc ccaggcggcg cagctcgtcg gcccagccca gcaccgtctc
      901 gaagttctgg tccacctcgg ggtccaccag ctcgacgtcg tcggtggtca cctggccctt
      961 ggtgccgtcg atggcgatca ggtcgccctc ctccagggtg tgctcgcccg ccttgacggt
     1021 cttggcgtcg aggtcgatct ccaggtcgcc ggcgcccgcc acgcacggct tgcccatgcc
     1081 ccgggccacc agcgcggcgt ggctggcctt gccgccctcg gcggtcagga tgcccttggc
     1141 ggcgtggaag ccgccgacgt cgtcggcctc ggtgaacggc cgcaccagga tcacgtcgcg
     1201 cccgtccttc tcggccgcga tggccgcgtc ggcggtgaac acgacctcgc ccttcgccgc
     1261 gcccggcgac gcgctcacgc ccttggccac cacctcgtac tcggcgtcgg gagcgaacgt
     1321 ggggtgcagc agcgcgtcca gtcggtcggg ctcgatcgtg gccagggctt cgcccttgtc
     1381 cagcaggccc tccttctcgg cgtcgacggc gaaccgcacc gccgcctggg cggggcgctt
     1441 ggcgctccgc gtctggagca tgaacagctg cccctcctcg accgtgaact cggtgtcctg
     1501 catgtcgccg tagtgcttct ccagcgtctg caggatctcc agcagctcgc cgtgggcgtc
     1561 gggcatcgac tccttcatct ccgagatgtc cttgggcgtg cgcacgccgg agacgacgtc
     1621 ctcgccctgg gcgttcacca ggtagtcgcc cgagggctcc ggcgcgccgg tgatctcgtc
     1681 gcgtgagaag gccacgcccg agcacgagga gtcgcccttg ttgccgaaca ccatctgctg
     1741 cacgttcacc gcggttcccc actcgtcggg gatccggttg atgcggcggt actccttggc
     1801 ccgcggcccg acccacgagt cgaacaccgc ccggatggcc tgggccagct ggtcgcgcgg
     1861 gtcctgcggg aagtcctcgc cggtctcgtc gcggtacagg tccttgaagt cgtcgaccag
     1921 ccccttgagg tcgtcgacct ccagctcggt gtccagctcc accccggcgt cggacttgcg
     1981 cttcttgatc gccttctcga tcgcctcgcc gctgaccccg cggcagacgt tggcgaacat
     2041 ctgcacgaac cggcggtagg agtcccaggc gaagcgctcg gagtcggtcg cgcgggccag
     2101 gccctcgacc gaggtgtcgt tcatgcccag gttcaggacc gtgtccagca tgccgggcat
     2161 ggactcgcgg gcgccggagc gcaccgagac cagcagcggg tcctcgtcat cgcccagctg
     2221 cttgcccgcg tgctcctcca ggcgctcgag cgccgcgtcc acctgctcct ccaggccctc
     2281 gggctccgtc cggtcggcct ccatgtacgc cacgcacgcc tcggtggtga tggtgaaccc
     2341 ggccgggacc cgctcggggc cgagcacgcg ggtcatctcg gccacgttgg ctcccttgcc
     2401 tcccagcaga tcgcgcatgt cgcgcgagcc ctcggcgaag tcgtacacca gctgcgtatc
     2461 cgcgttcaca ggcaagattg gagtctatgc ccgtctcaga gcagatcatt ctcgcccgtc
     2521 acggcgagac cgagtggagc cgctccggcc gccacaccgg ccgcaccgac atcccgctga
     2581 ccgaggaggg ccgccgccag gccgaggccc tccggcccgc gctcgcggac ctggagccgg
     2641 cgctggtgct gaccagcccg ctgtcgcgcg ccgccgagac ctgccgcctg gccgggctgg
     2701 gcgacgtggc cgtggaggag ccggcgctga tggagtggga ctacggcgac tacgaggggc
     2761 tgaccagcga cgagatccac gagaaccgac cgggctggaa cgtcttcacc gacggcgccg
     2821 ccgggggcga gaccccggac caggtcgcgg cccgcgtcga cggcgtgatc gagcgcatgg
     2881 ccggcgccgg cgggctgtgc gtggcgttcg cccacgggca cgtgctgcgc gtgctgggcg
     2941 cgcgctgggt cgagctgccg ccggccgcgg gcgcgcggct ggggctgtcg acggccacgc
     3001 tgagcgtcct cggcaccgag cacggcgtcc gggccctggc gcgctggaac gccgcgccct
     3061 gaggagagtg gccgccgccg ggcccgcacc cgggcggcgg ccagatactg cgcgaagccg
     3121 catcgtggcc tccgcgttcg gtatcccctc ctggaaccgg gtggtaccgg ggtcagggcc
     3181 gaagttcggc cgccgccctt gtgagccgtc acagaacccc ggtcagcgcc cgacgccggc
     3241 cagcgcctcg agcgcgcgct cgaagtgctc gtcgcggagc cggatgtggc tctgctggct
     3301 catcgaccgc gccgcgacgc cgatggcctc gaccggcggg gcgttggaga gcagcgggac
     3361 ggccaggagc ggctcgacgg gcacagtcca gggccagcgc cgcgggttcg acgccaccgg
     3421 gtccgcgtcc gtggggtcgc cgaccacctc cacgaccgcg aacaccaccc gccagaccga
     3481 cgcgtagtag accagccggt cgccgggctc catccgcgga cggcggggaa agccggtgcg
     3541 ccggagcgcc ccggcgcggc cgtcctcgat ccacgcgtcg ggcatggggc ccttcgcgtg
     3601 gcccatcgcc ttgagccagg gctgcaccgc tacgcgacgc ggcgcaggcg cacgtgggcg
     3661 tggtgctcca gcgtctccgc gatggcgcgg tcggcctggg ccagggcgcg cggctcggcc
     3721 gccggggcct gggccagcgg ccggccgacg gcgccgacca tgtactcgtg ggcggcggcg
     3781 tccagcggga acgacccggc ctcacaggcg gcgcacacca cgcccccggc cgccggcgag
     3841 aacgcgccca gcccctcccc ctcgccgcag gcggcgcacg agcccagctc gggggcgaag
     3901 ccggcggcca gcaggagctt catgcggaag gccagcatcc gctcctgcga ggcgccctcg
     3961 ggatcggcgt gcagcagcgc cagcttgttg cacagcaggt tgtaggcggc cgggttgggg
     4021 tcggtcgagt cgaacagctt gagcaccgcc tcacaggcct gggtcgcgtg cttgagggcg
     4081 gtccagtgct cgcgcgcgcc ggcgtgggcg tgcacggtgt ccaccgccga gatggtgcac
     4141 agctcgccgc ggccgcggtg cagcaccagc ttcacccgcg ccatcggctc cagccgcccg
     4201 cccaaccgcg acttggtccg ccggaccccc ttggccaccg cccccaccct gccgtgctcg
     4261 gggctgtaca ggtgcagcac ccggtccgcc tcgccgtacc ggatcgagcg caacaccacc
     4321 gcttcggttt tgaacgatcc gggcacaaat acactttgaa cgagatgcca gacatcacga
     4381 tgtacaccac caccccctgc ggcttctgca gccaggccaa ggcgctgctg gacgcgcgcg
     4441 gcttcgagta cgacgtcgtc gacatgagcc gcgaccccga ggcccgcgag gagctcgtgg
     4501 ccaggaccgg gcggatgacc ttcccccaga tcatggtcgg cgaccgcacg gtcggcggct
     4561 tccaggagct gctggccgcc caccgcgccg gcacgctcag cgaagtgctc gcggacgccg
     4621 cgtaagaagt tcctggcgaa agcggcccgg ctggtgcaga ctggccgggt gattcagggg
     4681 gtcacccgtt cgcggcacgt cgctgtagcc atcgtggcgg ccgcgctcct cctgacggcc
     4741 ctgtgcctgg ccggccgcgg cgacgcgcag ccgccgccca caccggagtt caccgactgg
     4801 acgacggcca ccgacaccaa ggtggacggg acgctccaag gcgcggcgat gagcctcacc
     4861 ggggagatcg gcccctccac cgtcaccaac ggcagcgaga cgggtttcaa caactccgcg
     4921 ttcaccccgc cgctggccac cagcgactcg ctgcaggtgc ggacggccac cgccggcaat
     4981 ccctacacct tcgtgttcac gcagcccgtg accaacccgg tgatccacat cggcgacctg
     5041 tccgggacgg tgtcgttcaa cccgtccgtg cgactgagcc gggtcagcgg cgacgccggc
     5101 ttcaccgtct cgggatccac ggtcacgggc accggcggcg ccaacggcac cgtggtcccg
     5161 atcgacacgt acaccggcct ggcgttcacc gccacctcca accccgccga tgtgttctac
     5221 ctgcagctgg gcgcgttccc cggggcggtg gggcggccgg cgcccccgcc cgacttccgg
     5281 gtccagcgcg ggtcaggcgg ccaggtcgtc gtggcgcccg gcgccagcac gcgcgtgccc
     5341 gtcgaggtgg cgcgcaactc gccgtcctcc ggtccgatca acctgagcag cctcgcccag
     5401 ggcaccggcg tgaccacgcg gatggagccg acccaggtca acgggcggga cctggagcgc
     5461 gcgacgctcg tggtcatggc cgccaagagc gccaaaccgg ggatcaggac cgtcacggtc
     5521 aggggctccc cgggcgcagc cggcgcggga cccacctccc ggacggtcca g
//
LOCUS       DASUPW010000148         3990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1696982, whole genome shotgun sequence.
ACCESSION   DASUPW010000148 DASUPW010000000
VERSION     DASUPW010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1696982"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..681
                     /locus_tag="VFY44_03355"
     CDS             325..681
                     /locus_tag="VFY44_03355"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5909503.1"
                     /translation="MVGGVATVWIPVQDVERAVTFYRDALEFEVKQQDETWAEVDANG
                     LMIGLNGSEPDGAGVSGGPVVAFQVEDELENAVEELKGRGVTFDTDISDHPWGRIANF
                     NDSEGNDLQLYQPPKG"
     gene            complement(678..1271)
                     /locus_tag="VFY44_03360"
     CDS             complement(678..1271)
                     /locus_tag="VFY44_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5909504.1"
                     /translation="MFDHVTIRAADPAASLRFYEATLGAAGIAAWPDFRIETGPSPTQ
                     RLHIGFSVAGRDHVHAFWDAGVAAGYTDDGAPGLRPEYTGDYYGGFLLDPDGNSAEAV
                     HHRGIRRSGIVDHVWMRVADLDASRRFYDSIATDAGYRLNKELPGRVAYVSERPDGGD
                     FSIVEGEPTRNAHLAFPAGRADALTDPDGNTVELVRA"
     gene            complement(1256..2293)
                     /locus_tag="VFY44_03365"
     CDS             complement(1256..2293)
                     /locus_tag="VFY44_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909505.1"
                     /translation="MAELPIPDHFDPARVGEVWRVPYQERSVEAERWAARHALPAAAN
                     DDARVGLLLVDVQNTFCVPGFELFVAGASGQAAVDDNRRLSEFVYRNLGTITQMVPSL
                     DTHHAMQIFHAAWLIDADGNRPDPMTLVTADDARDGRWRVDPQVAAAAGVDPGYAQRQ
                     LEHYTRQLADRGKYDLTVWPYHAMLGGIGHALVSSVEEAVFFHGLARRSAPRFCVKGE
                     LALTEHYSMLGPEVTEGPDGEAIGGTDHALVETLLGFDAVIVAGQAKSHCLAWTIDDL
                     LAHHGVQEDELARRTYLLEDCTSPIVVPGVVDYTGEADAAFERFAAAGMHVVRSTDPI
                     EDWPDFPCSTT"
     gene            2381..3175
                     /locus_tag="VFY44_03370"
     CDS             2381..3175
                     /locus_tag="VFY44_03370"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5909506.1"
                     /translation="MTTTTTEGSAARQGALWGARPADWAETEARQLPTYRTVIERVGL
                     APGDRVADLGCGAGTFLGLAAEHGARVAGLDAARSLLAIAAERVPGADLRQGDLERLP
                     WDDDRFDLVTGFNSFFFAADIGAALAEARRVARPGADVVIQVWGRPDRCDLGAMIAAM
                     GQLRDARPAAPAGPPLWQPGVVEHMARGAGLEPRLAFDYSCAFDYADEDELVRGMAAA
                     GGVVEAAAAVGDEPVRRAILDSLARFRAADGSYRLANEWRSVVATA"
     gene            complement(3179..>3990)
                     /locus_tag="VFY44_03375"
     CDS             complement(3179..>3990)
                     /locus_tag="VFY44_03375"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HEX5909507.1"
                     /translation="GGPTQTKLPAGSGPAIDAGTANLLTTDQRGLPRTQDRLPANVTD
                     GTDIGAVEIPGESPVTPGRPVTPSSTPTICGRRAISLVRADIRGNKVRLTGLVGSRFY
                     GKTVTIQTDPKGAKNSAFKKTGTVKASSTGAFTVSVPKPASDDIASVRYRAVIGSAKS
                     PALKLPQSLTSRSVKSAKGTITVKGKVKLSVLGKRNKVKIRRLVCGRYRTVGSATPDA
                     KGNYSITFKSTAIRGVAFYRAESKVLRKPGSKVYVIQYARAIAIKTTSQTG"
BASE COUNT          518 a         1374 c         1492 g          606 t
ORIGIN      
        1 ctgggcggct tcatcatgtt cgtgtactcg agcctgctgg tggtcaccaa ccgccgctac
       61 ctgccgaagt cgttgcagct cggtgggtac cggctcgcga tcatgggcat ctcgatcgtc
      121 gtcctgggcg ccacctcggt catcgtcggg atcgaccagt tcgggcagct gctgtgagcg
      181 cgcgcttcgg agcccgtctg ctgatcgccc tgttcgtgtg ggcggcgttc gtgggggtcg
      241 cgtacgcgct cggcctgggt tagaccgtca tccccgggca acgggtgctc gtagacgtac
      301 tttccccccg aagaaggagc accgatggtc ggtggagtag ccacagtctg gatccccgta
      361 caggacgtcg agcgcgcggt cacgttctac cgcgacgcgc tggagttcga ggtcaagcag
      421 caggacgaga cctgggccga ggtggacgcc aacggcctga tgatcggcct caacggcagc
      481 gagcccgacg gcgccggcgt ctccggtggt cctgtggtcg cgttccaggt cgaggacgag
      541 ctggagaacg cggtcgagga gctcaagggc cgcggcgtca cgttcgacac cgacatctcc
      601 gaccacccct ggggccggat cgccaacttc aacgacagcg agggcaacga cctccagctg
      661 taccagccgc cgaagggcta ggcccgcacc agctcgacgg tgttgccgtc ggggtccgtg
      721 agcgcgtccg cgcggccggc cgggaacgcc aggtgggcgt tcctggtcgg ctcgccctcg
      781 acgatcgaga agtcgccgcc gtcgggccgc tcgctgacgt aggccacgcg gccgggcagc
      841 tccttgttca ggcggtagcc ggcgtcggtc gcgatcgagt cgtagaagcg gcgcgacgcg
      901 tccaggtcgg ccacccgcat ccacacgtgg tcgacgatcc cgctgcgccg gatcccgcgg
      961 tggtgcaccg cctcggcgct gttgccgtcg gggtccagga ggaagccgcc gtagtagtcg
     1021 ccggtgtact cggggcgcag gccgggcgcg ccgtcgtcgg tgtagccggc agccacgccc
     1081 gcgtcccaga acgcgtgcac atggtcgcgg ccggccaccg agaagccgat gtgcaggcgc
     1141 tgcgtcgggg aggggccggt ctcgatccgg aagtcgggcc aggccgcgat gccggccgcg
     1201 cccagcgtcg cctcgtagaa ccgcagcgac gccgccgggt cggcggcgcg gatggtcacg
     1261 tggtcgaaca cgggaagtcc ggccagtcct cgatcgggtc cgtggagcgc accacgtgca
     1321 tgccggccgc cgcgaagcgc tcgaacgcgg cgtccgcctc gcccgtgtag tccacaacgc
     1381 ccgggaccac gatcggcgag gtgcagtcct ccagcaggta ggtgcgccgg gccagctcgt
     1441 cctcctggac gccgtggtgg gcgagcaggt cgtcgatggt ccaggccagg cagtggctct
     1501 tggcctggcc ggccacgatc acggcgtcga agcccagcag cgtctccacc agcgcgtggt
     1561 cggtgccgcc gatggcctcg ccgtcgggtc cctcggtcac ctcggggccg agcatcgagt
     1621 agtgctcggt cagcgccagc tcgcccttga cgcagaaccg cggcgcgctg cgccgggcca
     1681 gcccgtggaa gaacaccgcc tcctcgaccg acgagaccag cgcgtgcccg atcccgccca
     1741 gcatcgcgtg gtagggccac acggtcaggt cgtacttgcc ccggtcggcc agctggcgcg
     1801 tgtagtgctc cagctggcgc tgggcgtacc cggggtccac accggccgcc gcggccacct
     1861 gggggtccac gcgccagcgc ccatcgcgcg cgtcgtcggc ggtgaccagc gtcatcgggt
     1921 cggggcggtt gccgtcggca tcgatgagcc aggcggcgtg gaagatctgc atcgcgtggt
     1981 gggtgtccag gctgggcacc atctgcgtga tcgtgcccag gttccggtag acgaactcgg
     2041 acaggcggcg gttgtcgtcc accgccgcct gcccggacgc cccggccacg aacagctcga
     2101 agccggggac gcagaacgtg ttctgaacgt ccaccagcag aaggcccacg cgggcgtcgt
     2161 cgttcgcggc cgccggcagc gcgtgacggg cggcccaccg ctcggcttcg acggaccgct
     2221 cctggtaggg cacccgccag acctcgccca cgcgggcggg gtcgaagtgg tcggggatcg
     2281 gcagctcggc catcgggctc cgacgttatt cggtcttgcc aagactacgg tccatagtgt
     2341 atagctatga aaaatagttt cgagggagag gaggtccccg atgaccacga ccaccaccga
     2401 aggcagcgcg gcccggcagg gcgcgctctg gggtgcccgg ccggcggact gggccgagac
     2461 cgaggcgcgt cagctgccca cctaccgcac ggtgatcgag cgcgtcgggc tcgcgccggg
     2521 cgaccgtgtg gccgatctgg gctgcggggc cggcaccttc ctgggcctgg ccgccgagca
     2581 cggcgcgcgg gtggcggggc tggacgccgc ccggagcctg ctggccatcg ccgccgagcg
     2641 tgtgcccggc gcggacctgc gccagggcga cctggagcgg ctgccgtggg acgacgaccg
     2701 gttcgacctg gtcaccggct tcaactcgtt cttcttcgcg gccgacatcg gggcggcgct
     2761 ggccgaggcg cggcgggtgg cgcggccggg tgccgacgtg gtgatccagg tctggggccg
     2821 gcccgaccgg tgcgacctgg gcgcgatgat cgcggcgatg gggcagctca gggacgcccg
     2881 gcccgcggcc ccggcgggac cgccgctgtg gcagcccggg gtggtcgagc acatggcccg
     2941 cggcgccggg ctggagccgc ggctggcctt cgactattcg tgcgcgttcg actacgccga
     3001 cgaggacgag ctggtgcgcg gcatggccgc cgccggcggc gtggtggagg cggccgcggc
     3061 cgtcggcgac gagcccgtcc gccgcgcgat cctggactcc ctggcccggt tccgcgcggc
     3121 cgacggctcg taccggctgg ccaacgagtg gcgcagcgtg gtggctaccg cctgagcgtc
     3181 agccggtctg cgacgtcgtc ttgatcgcga tcgcgcgggc gtactggatc acgtagacct
     3241 tcgagccggg cttgcgcagc accttcgact cggcccggta gaaggccacc ccgcggatcg
     3301 cggtgctctt gaacgtgatc gagtagttgc ccttggcgtc cggcgtggcc gagcccaccg
     3361 tgcggtagcg gccacagaca agccggcgga tcttcacctt gttgcgcttg cccagcaccg
     3421 agagcttgac cttgcccttc acggtgatcg tgcccttggc cgacttgacc gacctcgagg
     3481 tcagcgactg cggcagcttc agcgccggcg acttggccga gccgatgacc gcgcggtagc
     3541 ggaccgacgc gatgtcgtcg ctggcgggct tgggcaccga gacggtgaac gcgccggtgc
     3601 tggacgcctt gaccgtgccg gtcttcttga aggcggagtt cttggcgccc ttcgggtcgg
     3661 tctggatcgt gaccgtcttg ccgtagaacc gcgagcccac gagccccgtc agcctgacct
     3721 tgttgcccct gatgtcggcc cggaccaggc tgatggcgcg gcggccgcag atggtcgggg
     3781 tcgagctggg ggtcacgggg cggccgggcg tgaccgggct ctcgcccggg atctccacgg
     3841 cgccgatgtc ggtgccgtcg gtcacgttgg ccggcagccg gtcctgtgtg cggggcagcc
     3901 cgcgctggtc ggtggtcagg aggttcgccg tgccggcgtc gatggccggg cccgatcccg
     3961 ccgggagctt ggtctgcgtc ggcccgccgt
//
LOCUS       DASUPW010000149         2960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1836298, whole genome shotgun sequence.
ACCESSION   DASUPW010000149 DASUPW010000000
VERSION     DASUPW010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1836298"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VFY44_03380"
     CDS             complement(<1..617)
                     /locus_tag="VFY44_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5909508.1"
                     /translation="MPEFALQGKVALVTGAARGIGLETARLLHQRGASVVVCDIDRAA
                     TERAAGQIGERALAFPADVTDRDAMAAAVDAAVVTYGALDVVVANAGVGPPGQTMKAM
                     DLSMFERTLEIDLLGVVRTVHPALDPIARRGGQVVVISSVYAFATGSLVTPYAAAKAG
                     VEALGRALRVELAPHGAGATVAHFGFVDTSMVQEATEKSERDDVL"
     gene            635..1222
                     /locus_tag="VFY44_03385"
     CDS             635..1222
                     /locus_tag="VFY44_03385"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEX5909509.1"
                     /translation="MEQALPLIVATGWASGVNAYATVALMCVCQRADLIELPAAFART
                     DVLVVSLALFAIEFVADKVPLVDSAWDVFQTVARPLVGGTIGALVAGEDGATALVEAG
                     AAGGSGGLALASHSVKASIRLAVNTSPEPFSNVAVSLAEDLAVALVVWLATDHPWLAA
                     GVAVALLVCGVTVALFLAKAVRAGLRRLRARRAPS"
     gene            complement(1337..1720)
                     /gene="rplL"
                     /locus_tag="VFY44_03390"
     CDS             complement(1337..1720)
                     /gene="rplL"
                     /locus_tag="VFY44_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX5909510.1"
                     /translation="MATSTDEWIDELKGISVLELSERIKALEEAFGVSATAVAAPAAG
                     GGGGGGEAAAEEESSTVDVVLTGAGDKKIQVIKVVRAATGLGLKEAKALVDEAPKPIK
                     EGIEREEADKLKADVEEAGGTVEVK"
     gene            complement(1723..2460)
                     /gene="rplJ"
                     /locus_tag="VFY44_03395"
     CDS             complement(1723..2460)
                     /gene="rplJ"
                     /locus_tag="VFY44_03395"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX5909511.1"
                     /translation="MNREEKAAVIDQVATQIQEAEAIYAIDYRGISVTQVAELRSKLA
                     ESDASLRVVKNSLTELAADKAGAEHLKEHLEGPTAFTFVRGDAALAAKAIANFRKEYE
                     LLSFKGGVMGGEVLTADQIEDIAKLPAREVLDGRLVGILASPVTGLVNGLNNLIQGLA
                     LQLGQIAEQGLVTGEAPAPEPEPEAPAEEPAAEAEAPAAEAEAPAEETDSADEGATAE
                     ADADAEAPAAEETESNEEPSEGEDKEG"
     gene            complement(2604..>2960)
                     /locus_tag="VFY44_03400"
     CDS             complement(2604..>2960)
                     /locus_tag="VFY44_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX5909512.1"
                     /translation="GRLGRVLGPQGKMPNPKVGTVTPDVAKAVSESKAGKLEYRTDRH
                     AIVHLPIGKADFEESALVENYTALIDEIVRAKPSAAKGRYLHTVTITTSMNPGVKVDP
                     GATASAAAVEPAEAVA"
BASE COUNT          348 a         1053 c         1065 g          494 t
ORIGIN      
        1 tccaggacgt cgtcgcgctc gctcttctcg gtggcctcct ggaccatgct ggtgtccacg
       61 aacccgaagt gggcgaccgt ggcgccggcg ccgtgcggcg ccagctccac ccgcagcgcg
      121 cgccccaggg cctcgacccc cgccttggcg gcggcgtagg gcgtgaccag cgatccggtt
      181 gcgaacgcgt agaccgacga gatgaccacg acctgtcccc cgcgccgggc gatcgggtcc
      241 agcgccgggt gcacggtccg caccacgccg agcaggtcga tctccagcgt gcgctcgaac
      301 atcgagaggt ccatggcctt catcgtctgg ccgggaggac cgacgccggc gttggcgacc
      361 accacgtcga gggcgccgta ggtgaccacg gccgcgtcga ccgccgcggc catggcgtca
      421 cgatcggtca cgtccgccgg gaaggccagc gcgcgctcgc cgatctggcc cgccgcccgc
      481 tccgtggctg cgcggtcgat gtcgcacacc accaccgagg cgccacgctg gtggaggagg
      541 cgggccgtct cgagcccgat cccccgggcc gcgcccgtta ccagcgcgac cttcccctgt
      601 agcgcgaact cgggcattgc ccgatcctat ggcgatggaa caggcgctgc cgctgatcgt
      661 ggccaccgga tgggcgtcgg gggtgaacgc ctacgcgacc gtcgctctga tgtgcgtgtg
      721 ccagcgcgcg gacctgatcg agctgccggc ggccttcgcg cgcaccgacg tcctggtggt
      781 gtcgctggcg ctgttcgcga tcgagttcgt ggccgacaag gtgccgctgg tggacagcgc
      841 gtgggacgtg ttccagacgg tggcgcggcc gctggtgggc ggcacgatcg gcgcgctggt
      901 ggcgggcgag gacggcgcca ccgcgctggt cgaggccggg gcggccggcg ggtcgggcgg
      961 gctggcgctg gccagccact cggtcaaggc cagcatccgc ctggccgtga acacctcgcc
     1021 cgagccgttc tccaacgtgg ccgtcagcct ggccgaggac ctggcggtgg cgctggtggt
     1081 ctggctggcc accgaccacc cctggctcgc cgccggggtc gcggtggcgc tgctggtctg
     1141 cggcgtgacc gtggcgctgt tcctggccaa ggcggtgcgg gcggggctgc ggcggctgag
     1201 ggcccggcgc gcgccgtcct agaacgacga agggccggca agaagccggc cctcagacgt
     1261 acgaagcgcg ttctgcagcc gtcggacggc caccggacgc gcgggggttc cggggggcgc
     1321 gagccccccg gcccgattac ttgacctcga ccgtgcctcc ggcctcttcg acgtcggcct
     1381 tgagcttgtc ggcctcctcg cgctcgatgc cctccttgat gggcttcggc gcctcgtcga
     1441 ccagggcctt ggcctccttg aggcccaggc cggtcgccgc gcggacgacc ttgatgacct
     1501 ggatcttctt gtcgccggcg cccgtgagca cgacgtcgac cgtgctggac tcctcctcgg
     1561 cagcggcctc gccgccaccg ccgccgccac cggcggccgg ggcggccacc gccgtcgcgg
     1621 agacgccgaa ggcctcctcg agagccttga tgcgctcgga cagctcgagc accgagatgc
     1681 ccttgagctc gtcaatccat tcgtcagtgc tggtggccat ttctagccct ccttgtcttc
     1741 gccctcagag ggctcttcgt tgctttcagt ttcttcggcg gcgggggctt cggcgtcagc
     1801 atcagcttca gctgtcgctc cctcgtctgc agaatcggtt tcctcggccg gtgcctccgc
     1861 ctcggccgcc ggtgcctcgg cctcggctgc gggctcctcg gccggggcct cgggctcggg
     1921 ctcgggcgcc ggcgcctcgc cggtgaccag gccctgctcg gcgatctggc cgagctgcag
     1981 ggccaggccc tggatcaggt tgttcaggcc gttgaccagg ccggtgaccg gcgaggccag
     2041 gatgcccacc aggcggccgt cgagcacctc gcgcgccggc agcttggcga tgtcctcgat
     2101 ctggtcggcg gtcagtacct cgccgcccat gaccccgccc ttgaaggaga ggagctcgta
     2161 ctccttgcgg aagttcgcga tggccttcgc ggccagcgcg gcgtcgccgc ggacgaacgt
     2221 gaaggccgtc gggccctcca ggtgctcctt caggtgctcg gcgccggcct tgtcggcggc
     2281 gagctcggtg agcgagttct tgaccacccg cagggaggcg tccgactcgg ccagcttgga
     2341 gcgcagctcg gcgacctggg tcacggagat cccgcggtag tcgatggcgt agatcgcctc
     2401 ggcctcctgg atctgcgtgg ccacctggtc gatgaccgct gctttctctt ctctgttcaa
     2461 cgcgttgtcc tatctccgga acgaaatgag cccgcacgca ggcgagctcg ggaagaaggg
     2521 acttcgcggc ttcgcctgca ccgggcttat ggagaaaccc gccggtggtc tggggctgat
     2581 gttcagctgt tgcagcgtag cggctaggcc actgcctccg cgggctcgac cgccgcggcg
     2641 ctcgcggtgg cgcccggatc gaccttcacg cccgggttca tggacgtggt gatggtcacg
     2701 gtgtgcaggt agcggccctt ggcggcggac ggcttggcgc gcacgatctc gtcgatcagc
     2761 gcggtgtagt tctccaccag cgcgctctcc tcgaagtccg ccttgccgat cggcaggtgc
     2821 acgatggcgt gccggtcggt gcggtactcg agcttgccgg ccttggactc ggacacggcc
     2881 ttggccacgt ccggggtcac ggtgccgacc ttggggttgg gcatcttgcc ctgcggcccg
     2941 agcacgcggc ccaggcgacc
//
LOCUS       DASUPW010000150         6591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1143498, whole genome shotgun sequence.
ACCESSION   DASUPW010000150 DASUPW010000000
VERSION     DASUPW010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143498"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VFY44_03405"
     CDS             <1..214
                     /locus_tag="VFY44_03405"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5909513.1"
                     /translation="DNTMEIGGEKVQLGIPEQMRAMASMIIPLGRPGTPEEAAGGIFF
                     LCSPWSNYVHGQVLNITGGQFGGMIT"
     gene            complement(424..1857)
                     /locus_tag="VFY44_03410"
     CDS             complement(424..1857)
                     /locus_tag="VFY44_03410"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5715 family protein"
                     /protein_id="HEX5909514.1"
                     /translation="MARPRLLALGAIVVLIAAAGAWFALRDRGLPAADPPAALDAEAP
                     DPFAYTDARRTEFEQRATAGFARVVYAKSPGGVVATGARVAAYRDRIERVAADSGIPA
                     ETLEAIVFLESAGRPSAAADPKLEGAVGLTQILAETGQNLLDMRVDPAAARKLTRRID
                     RAERRGQTGRADRLRARRRKVDQRFDPDASLRATARYLNLAKGQLAGRDDYAVAGYHM
                     GIGNLQSVLRDYGSDPGNYTALYFGSTPTSRPAAYRRLAGLGDDSSTYLWRVKASESI
                     MRRYREDRAALEREDELQRAKNSSEEVLHPEGDSESFGTPDDLEAAYRDGKVRSFPNA
                     PARLGLRRDRQMGELARKLEQPRRLYRGLRPEAYALAAYAARMTRQAGGGRSPLIVTS
                     TVRDREYQRVLIGRNREATQNYSLHTTGYSFDVLRRYSGGRQAQAFQYTLTRLQALNL
                     IAWVREPAAIHITVSGDAKRLVPLLEG"
     gene            complement(1949..2620)
                     /locus_tag="VFY44_03415"
     CDS             complement(1949..2620)
                     /locus_tag="VFY44_03415"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5909515.1"
                     /translation="MSDLMKLDVVDTDGAIREAEEAVAGDSRADFFRKAALGGAATIG
                     GATLLGGFPALAMGKPSTKQDIEILNYALTLEYLENEFYKAAIKSGVIKTPGVLATAK
                     LVQADEAAHVAALPATIKKLGGKAVKKPKFDFSKAIGTEKAFLDTAVVLEDTGVAAYA
                     GQGPRLKTKAVVVAALGIHSVEARHASALRVLAGKKPVISPVDKPKSMKTVLAAVKKT
                     GFIKS"
     gene            2774..3679
                     /locus_tag="VFY44_03420"
     CDS             2774..3679
                     /locus_tag="VFY44_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEX5909516.1"
                     /translation="MAATAAEPQPAKLPLPGGSKGAAVQLHPLICARMHCAPGFLDRP
                     EGRLASLKTLVSRTSEDDLVDIPVVAFLVRHPSAGDVLVDTGLHPSVAVEPKNALGKW
                     GGLLFKDIRMATEDSIPAQLRALDVDPNTVRTVVMTHLHADHAGAVSEFPGATFVVDA
                     REWSAAADGSERDGYVRRQFDHGFDWRIVDFDGPTGDSFATFGRSVDLFGDGSVRLVS
                     TPGHSAGHVSVVLRLRDREALLCGDAAYTLDAIENDTVPYVVQDEHRYRRSLREIQLY
                     LEQTPDALVVPGHELAVMRRLDRVY"
     gene            complement(3689..6298)
                     /locus_tag="VFY44_03425"
     CDS             complement(3689..6298)
                     /locus_tag="VFY44_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909517.1"
                     /translation="MGGMRAWGACAVVVFATTAALSDAEPAAARPAAAAQVLTTSQAN
                     TLRLGYVRVRLDARRAGSVRLWSIAARGARGRSYRVTRSRLVRVRRPGSRVVGLRLSA
                     AGRRILGRCAAVALRPKATVRAKGRRRSVKLTGSSRAVRADAARCRATGAGRPAQGLA
                     APDPDPAPLGEVRTENADRCDFLDPAACLFPWPNDRFTKAAATPTGRQLDLNAQSVPR
                     NKLGNPIDPTDQNRADGFSPGNLIVTRVPGLDSQAAFDRTGAVPITDMARTYDPGQPV
                     VVINARTRKRQLIWAEVDSNPADPSDVTLIIRPGRNFDEGERYIVALRDLRRADGTTI
                     APREEFRAYRDALATSDPDIEARRAHHEDLFRTLAGAGIGRRNLYLAWDFTVASRQSL
                     TDRALSIRDDAFAQLGDTDLADSRIQGDSPTFVRNPDLPDGLTEQLPDAPLFDFKDLD
                     GYREFTPAQNATTARRITGTITVPCYLNLPGCPPGSSFSLGADGKPQRLPANTTTANV
                     ICTVPRKAVDQGAASPRARISLYGHGLLGSAGEVGGSSAQPFANDQNIVFCATDWIGM
                     STFDVPNVLTLLQDLSNFRSLVDRSQQGFVNFMYLGRWMSHPGGAAALDDFKVGGRSV
                     VDPSERLVYDGNSQGGILAGGLTALSPDFDRAVHGVPGMNYSTLLRRSVDFDTYAHGE
                     FEGVNSGLGLYQAYPDELERPLVLSLMQLLWDRGEANGYAHHMTDDPLPGTQPHQVLL
                     HPAFGDHQVANVAADVEARTIGARAHRPALFAGRSPDVTPLYDVPTAPAAWTGSGIVY
                     WDTGPLRDGGARGTPAPPAGNVPPRQGNDPHSAPRKDPKSWQQKSDFLRVGGTLTNPC
                     GLGPCFADGFAGP"
     gene            complement(6365..>6591)
                     /locus_tag="VFY44_03430"
     CDS             complement(6365..>6591)
                     /locus_tag="VFY44_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909518.1"
                     /translation="RNWKRLQFPKVTVQFGDAIQFDSVAEPTREQSQAASEQIMVRIR
                     GLWDGLRERGRSASIKAARSARRGTVVSGH"
BASE COUNT          832 a         2321 c         2501 g          937 t
ORIGIN      
        1 ggacaacacc atggagatcg ggggcgagaa ggtgcagctg ggcatccccg agcagatgcg
       61 cgcaatggcc tcgatgatca tccccctggg ccgcccgggc accccggagg aggccgccgg
      121 cggcatcttc ttcctgtgct cgccgtggtc gaactacgtg cacggccagg tgctgaacat
      181 caccggcggc cagttcggcg gaatgatcac ctgaggccgg ggcggcacgc tccgaccgcc
      241 cactccggga ccggcgcgcg tcacggaaga gcgcgaacgg tgacaagacc ctcgcccgac
      301 ccagcccaag ccgttacgcc gagcccgcag gggcggcccc gccgcgaccg gaacggttgt
      361 cggtttggcg gggtcccagc cccaccaagg cgacaagcgt tccagcgggg cccgaacccg
      421 acgtcagccc tccagcaacg gcaccagccg cttcgcgtca cccgagaccg tgatgtggat
      481 cgccgccggc tcccggaccc aggcgatcag gttcagcgcc tggagccggg tcagcgtgta
      541 ctggaacgcc tgagcctggc ggccgccgct gtagcgccgg agcacgtcga aggagtagcc
      601 ggtggtgtgc agcgagtagt tctgcgtggc ctcgcggttg cgcccgatca gcacccgctg
      661 gtactcgcgg tcgcgcaccg tgctggtgac gatcagcggt gagcggccgc cgccggcctg
      721 gcgcgtcatg cgcgcggcgt aggcggccag cgcgtaggcc tcgggccgca gcccgcggta
      781 cagccgccgc ggctgctcga gcttcctggc cagctctccc atctggcggt cccggcgcag
      841 ccccagccgc gccggcgcgt tggggaacga gcgcaccttg ccgtcgcggt aggccgcctc
      901 gaggtcgtcc ggggtgccga aggactcgga gtcgccctcg gggtgcagca cctcttcgga
      961 ggagttcttg gcccgctgga gctcgtcctc gcgctccagc gcggcgcggt cctcgcggta
     1021 gcggcgcatg atcgactcgg atgccttgac ccgccacagg taggtggacg agtcgtcgcc
     1081 cagccccgcc aggcgccgat aggcggccgg ccggctcgtg ggagtgctcc cgaagtagag
     1141 cgcggtgtag ttgcccgggt cggagccgta gtcgcgcagg accgactgga ggttgccgat
     1201 gcccatgtgg tagccggcga cggcgtagtc gtcgcgcccg gccagctggc ccttggccag
     1261 gttcaggtag cgggcggtgg cgcgcagcga ggcgtcgggg tcgaagcgct ggtcgacctt
     1321 gcgccggcgc gcccggaggc ggtcggcgcg gccggtctgg ccccggcgct cggcgcggtc
     1381 gatgcggcgg gtcagcttcc gcgccgcggc cgggtccacc cgcatgtcca gcaggttctg
     1441 cccggtctcg gccaggatct gggtcagccc gaccgcgcct tcgagcttgg ggtccgcggc
     1501 ggccgacggc cggccggcgc tctccaggaa gacgatcgcc tcgagggtct cggcggggat
     1561 gccgctgtcg gccgccacgc gctcgatccg gtcgcggtac gcggccaccc gggcgcccgt
     1621 ggccaccacg ccgcccgggc tcttggcgta caccacgcgg gcgaagccgg ccgtggcacg
     1681 ctgctcgaac tccgtccggc gggcgtccgt gtaggcgaac gggtcgggcg cctcggcgtc
     1741 cagcgccgcg ggcgggtcgg ccgccgggag gccccggtcg cgcagggcga accaggcgcc
     1801 ggcggcagcg atcagcacga cgatcgcgcc gagcgcgagt agacgggggc gggccaccgg
     1861 gccgaccgta gcgatcgcgg gtcgtccgca gaacgcagag agggcgcccg gaggcgccct
     1921 ctcggcacta cgtcccagga ggggggactt agctcttgat gaagccggtc ttcttcacgg
     1981 ccgcgagcac ggtcttcatg ctcttgggct tgtccaccgg cgagatgacc ggcttcttgc
     2041 cggccagcac gcgcagcgcg gacgcgtgac gggcctcgac cgaatggatg cccagggcgg
     2101 ccacgacgac ggccttggtc ttcagccgcg ggccctgtcc ggcgtaggcg gcgacgccgg
     2161 tgtcctcgag gacgacggcg gtgtccagga acgccttctc ggtgccgatg gccttggaga
     2221 agtcgaactt cggcttcttg acggccttgc cgccgagctt cttgatcgtc gccggcaggg
     2281 cggccacgtg ggcggcctcg tcggcctgga ccagcttggc ggtggcgagc acgcccgggg
     2341 tcttgatgac gccgctcttg atggccgcct tgtagaactc gttctcgagg tactcgaggg
     2401 tgagcgcgta gttcaggatc tcgatgtcct gcttggtcga gggcttgccc atcgccagag
     2461 cggggaaccc gcccagcagc gtggcgccgc cgatcgtggc cgcgccgccg agggcggcct
     2521 tgcggaagaa atcggcgcgg ctgtcaccgg cgacggcctc ttcggcctcg cggatggcac
     2581 cgtcggtgtc tacgacgtcc agcttcatca gatcggacat gtgaggtttc gtgctccctt
     2641 gtcgggttgg ggaactgcgt gcttctccct gtgctggcgc gccccgggag ccttcggatc
     2701 accgcccggg ccggttggcg cgagcccgct tgtggcttcg cgcccagccg ccccgccatc
     2761 tatggtcccg gccatggcag ccaccgccgc tgagccgcaa cccgccaagc tgccgctgcc
     2821 ggggggctcg aagggggccg cggtgcagct gcacccgctg atctgtgcgc ggatgcactg
     2881 cgcgcccggg ttcctggacc gccccgaggg gcggctggcg tcgctgaaga ccctggtctc
     2941 gcgcacatcc gaggacgatc tggtggacat ccccgtggtg gccttcctgg tccgtcaccc
     3001 cagcgcgggc gacgtgctgg tggacaccgg actgcaccct tcggtcgcgg tcgagcccaa
     3061 gaacgccctg ggcaagtggg gcgggctgct gttcaaggac atccgcatgg ccaccgagga
     3121 ctccatcccc gcgcagctgc gcgcgctgga cgtggacccg aacacggtcc gcaccgtggt
     3181 catgacccac ctgcacgccg accacgccgg cgccgtcagc gagttcccgg gggccacctt
     3241 cgtggtcgac gcgcgcgagt ggtcggccgc ggccgacggc tccgagcgcg acggctacgt
     3301 gcgacgccag ttcgaccacg gcttcgactg gcgcatcgtg gacttcgacg gcccgacggg
     3361 ggactcgttc gcgaccttcg gccgctcggt cgacctgttc ggcgacggca gcgtgcggct
     3421 ggtctccacc cccggccaca gcgccgggca cgtgtcggtg gtgctgcgcc tgcgcgaccg
     3481 cgaggcgctg ctgtgcggcg acgccgccta caccttggac gcgatcgaga acgacaccgt
     3541 gccctacgtc gtccaggacg agcaccgcta ccggcgctcg ctgcgcgaga tccagctgta
     3601 cctggagcag acgccggacg cgctggtggt gcccgggcac gagctcgcgg tcatgcgcag
     3661 gctcgaccgc gtctactgag cggcggggct agggcccggc gaacccgtcg gcgaagcacg
     3721 gcccgagccc acacgggttg gtcagcgtgc cgccgacgcg gaggaagtcc gacttctgct
     3781 gccaggactt ggggtccttg cgcggcgccg agtgggggtc gttgccctgc cgcggcggca
     3841 cgttgccggc cggcggcgcg ggcgtgcccc tggcgccgcc gtcgcgcagc gggccggtgt
     3901 cccagtagac gatgcccgac cccgtccagg ccgccggcgc cgtgggcacg tcgtacaggg
     3961 gcgtcacgtc gggcgagcgg ccggcgaaga gcgccggccg gtgggcacgc gcgccgatgg
     4021 tgcgggcctc gacgtcggcg gccacgttgg ccacctggtg gtcgccgaac gcggggtgca
     4081 gcagcacctg gtgcggctgg gtgcccggca gcgggtcgtc ggtcatgtgg tgcgcgtagc
     4141 cgttggcctc gccgcggtcc cacagcagct gcatcagcga cagcaccagc gggcgctcca
     4201 gctcgtcggg ataggcctgg tagagcccca gccccgagtt cacgccctcg aactcgccgt
     4261 gggcgtaggt gtcgaagtcc acgctgcggc gcagcagggt ggagtagttc atgcccggca
     4321 cgccgtgcac ggcgcggtcg aagtcggggg acagggcggt cagcccgccg gccaggatgc
     4381 cgccctggct gttgccgtcg tagaccaggc gctcggacgg gtcgacgacc gagcggccgc
     4441 ccaccttgaa gtcgtccagc gcggcggcgc cgccggggtg gctcatccac cgccccaggt
     4501 acatgaagtt cacgaagccc tgctggctgc ggtccaccag cgagcggaag ttggacaggt
     4561 cctggagcag ggtcagcacg ttgggcacgt cgaaggtgga catgccgatc cagtcggtcg
     4621 cgcagaagac gatgttctgg tcgttggcga acggctgcgc gctggagccc ccgacctcgc
     4681 ccgccgaccc cagcaggccg tggccgtaga gcgagatgcg cgcccgcggc gacgcggccc
     4741 cctggtcgac ggccttgcgc ggcacggtgc agatcacgtt ggccgtggtg gtgttcgcgg
     4801 ggagccgctg cggcttgccg tcggcgccca ggctgaacga cgacccgggc gggcagccgg
     4861 gcaggttcag gtagcacggc acggtgatcg tgccggtgat gcggcgggcg gtggtggcgt
     4921 tctgggcggg cgtgaactcg cggtagccgt ccaggtcctt gaagtcgaac aggggcgcgt
     4981 cgggtagctg ctcggtcagg ccgtcgggga ggtcggggtt gcgcacgaac gtcggcgagt
     5041 cgccctggat ccggctgtcg gccaggtcgg tgtcgcccag ctgggcgaac gcgtcgtcgc
     5101 ggatcgacag cgcccggtcg gtcaggctct ggcggctggc caccgtgaag tcccacgcca
     5161 ggtacaggtt gcgccgcccg atgcccgccc cggccagcgt ccggaacagg tcctcgtggt
     5221 gtgcccggcg ggcctcgatg tcggggtcgg aggtggccag cgcgtcgcgg taggcacgga
     5281 actcctcgcg cggcgcgatg gtggtgccgt cggcgcgcct caggtcgcgc agggcgacga
     5341 tgtagcgctc gccctcgtcg aagttgcgcc ccggccggat gatcagcgtc acgtccgatg
     5401 ggtccgccgg gttggagtcc acctcggccc agatcagctg gcgcttgcgc gtgcgggcgt
     5461 tgatgaccac cacgggctgg ccggggtcgt aggtgcgcgc catgtcggtg atgggcacgg
     5521 cgcccgtccg gtcgaaggcc gcctggctgt ccaggccggg cacgcgggtg acgatcaggt
     5581 tgcccggcga gaagccgtcg gcgcggttct ggtcggtcgg gtcgatcggg ttgccgagct
     5641 tgttcctggg cacggactgg gcgttgaggt ccagctggcg gccggtgggg gtggcggccg
     5701 ccttggtgaa gcggtcgttg ggccacggga acaggcaggc ggccgggtcc aggaagtcgc
     5761 accggtcggc gttctcggtg cgcacctcgc ccagcggcgc cgggtcggga tcgggcgcgg
     5821 ccaggccctg ggcgggacgg ccggcgccgg tggcccggca gcgcgcggcg tcggcgcgga
     5881 cggcccggct cgagccggtc agcttcacgc tcctgcgccg ccccttggcc cgcaccgtcg
     5941 ccttcggcct gagggccacc gccgcgcagc gccccaggat ccggcggccg gccgcgctca
     6001 ggcgcagccc gaccacgcgc gagcccgggc ggcgcacgcg caccaggcgc gagcgcgtga
     6061 cgcggtagga gcggcccctc gccccgcgcg cggcgatgct ccacagccgc acgctgccgg
     6121 cgcggcgggc gtccagccgg acgcgcacgt agcccagccg cagcgtgttc gcctggctgg
     6181 tggtcagcac ctgcgcggcg gcagccggcc tggccgcggc cggctcggca tcgctcagcg
     6241 cagcggtcgt cgcgaacacg acgacggcgc acgcacccca ggcacgcatc cctcccacgc
     6301 cctggatggt gaacgagcgc tgcccgcagg tctacgggca acctccgaaa tccgcgcgcc
     6361 ggttctagtg accgctcaca acggtgccgc ggcgggcgga tcgcgccgcc ttgatcgacg
     6421 ccgaccggcc acgctcgcgc aggccgtccc acaggccgcg gatgcggacc atgatctgct
     6481 cggacgcggc ctgggactgc tcacgcgtgg gctcggccac ggagtcgaac tggatcgcgt
     6541 cgccgaactg cacggtgacc ttcgggaact gcagccgctt ccagttgcgc a
//
LOCUS       DASUPW010000151         5091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_589701, whole genome shotgun sequence.
ACCESSION   DASUPW010000151 DASUPW010000000
VERSION     DASUPW010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589701"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VFY44_03435"
     CDS             complement(<1..500)
                     /locus_tag="VFY44_03435"
                     /inference="COORDINATES: protein motif:HMM:NF045177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909519.1"
                     /translation="MSVSGVEINGPDVARGDEVLTDEALELLAALHREINGRRKELLA
                     KRLEVQADLDAGGTLDFLEETKEIRDGDWQVSPAPPALRKRWVEITGPTSAKMVINAL
                     NSGSTGFMADFEDANSPSWANMVGGQANLKDAIRGTLEFDDEAKGKQYRLNEETATLL
                     VRPRGW"
     gene            complement(497..1777)
                     /locus_tag="VFY44_03440"
     CDS             complement(497..1777)
                     /locus_tag="VFY44_03440"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX5909520.1"
                     /translation="MEAGPIQIGMWDEDKPPEPSLIDDCVHCGFCLPTCPTYQLWGEE
                     ADSPRGRIVLMRAGHEEETELSGEMVGHFDNCLGCMACVTACPSGVQYDKLIEDTRAQ
                     VERKWERSGQDKWTRKIVFALFPHPERMRWLVPLMRFGAATKLDRLTASLPLGRIAEV
                     AKLAPAPPVRHALPEHVAARGESRGTVAVLRGCVQRVMFSGVNADTAAVLAAEGWDVE
                     APQDPSCCGALQLHAGMGTDATANVKKTIEALEGYDRIVANAAGCGSAMKDYGHVMRQ
                     DPDWAERAQAFAEKVVDVTEILAEGEPRAHRNPIEMKVAYHDACHLAHAQAVRTQPRD
                     LLRGIPGVELVEPAEWELCCGSAGIYNVVKPEAAAELGRRKAQNLLDTGAEAVVAANP
                     GCTLQITAHTRELGKELPVIHPVELLARSLRGDR"
     gene            complement(1777..2853)
                     /locus_tag="VFY44_03445"
     CDS             complement(1777..2853)
                     /locus_tag="VFY44_03445"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5909521.1"
                     /translation="MSAAVVEKLRDATEAGSTVRFAGGRTKFDWGGPAPADVEISTLE
                     LDRIVEHNVGDLTAVLDAGVRLADAQAAFAAEGQMLALDPPDLGATVGGVIATADSGP
                     LRHRYGSVRDLVVGMTVALSDGTVAKSGGKVIKNVAGYDLAKLFAGAYGTLGAILQVA
                     VRLHPLAPATVTAVGRSDDPRAVAAAASALTHARLELECLDVGWEDGSGAVMARSGGA
                     AAAEAAEHVSGLLAAEGLDASVDDDDGALWQAQRDAQRSEAQTVVRVSGLQDQTALLL
                     ELAQELDARVAGRAAYGLSWVTVAEPGYAERVRDALSPSPCAVLDGRGDLDAWGPRDP
                     GALALGERVRERFDPAGVCAPGLI"
     gene            complement(2850..4304)
                     /locus_tag="VFY44_03450"
     CDS             complement(2850..4304)
                     /locus_tag="VFY44_03450"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5909522.1"
                     /translation="MSADPALVAKLRAACGDEHVLTHHHQLRTYESDGLLQYAALPSA
                     VALPETAEQVQACVKACHEHEAPWVARGSGSGLSGGAMPVEDGVVIAVSRMRRILEVD
                     LANQRVVVEPGVTNIAVSEAVGPGHFYPPDPSSQIVCSIGGNVAENSGGAHCFKYGFT
                     TNYVTGLEVVLPDGQMVQLGGKGADIPGLDLVGAFVGSEGTLGVATKITLRVVPSPEA
                     VRTLVAFFDSTGEAGEVVSAMVSEGIVPGAIEMMDNLSIKAAEGATNAGYPQDAGAAL
                     VVELDGSEHECEARFDAVVKMCEDGGSWQVRVAQDEEERALIWKTRKAAFAAMGRIAP
                     NYYVQDSVIPRTRMAAVLRRIEELGAEYELQVANVFHAGDGNLHPLVCYDGSVEGEPE
                     RAEELAGMIVKACVDEGGSITGEHGVGVDKKRYMPSMFGEDDLATFQRLRCAFDPDGL
                     ANPGKVMPTPRLCGEVPGPYRKHPLEEAGIAERM"
     gene            complement(4301..>5091)
                     /locus_tag="VFY44_03455"
     CDS             complement(4301..>5091)
                     /locus_tag="VFY44_03455"
                     /inference="COORDINATES: protein motif:HMM:NF014683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEX5909523.1"
                     /translation="ETDTRRERWEAFAPYIIIVTVFAIVKLIDPVEKFLFELSGGTGF
                     DTDDKTVNGFDWPGLDVVTPDGEAASAVTFAFSFWDTPGTVVLFCGLLTVLAIKVPIR
                     DALRTYGETLKQLTLAIVTVMAVLGLAYVMNLSGMTSTLGLWIAGAGGILALLSPVIG
                     WLGVAITGSDTSSNALFGALQVQAAKEAGIDQTLLAAANSSGGVLGKMISPQNLAIGA
                     AAVGMAGKEGDIFRRVLFWSLVMTALMGILVYLQSTSILGWMVV"
BASE COUNT          681 a         1915 c         1763 g          732 t
ORIGIN      
        1 tgccacccgc gcgggcgcac cagcagcgtg gcggtctcct cgttcaggcg gtactgcttg
       61 cccttggcct cgtcgtcgaa ctccagcgtg ccgcggatgg cgtccttcag gttggcctgg
      121 ccgccgacca tgttggccca ggagggcgag ttggcgtcct cgaagtcggc catgaagccg
      181 gtggagccgg agttcagcgc gttgatgacc atcttggccg acgtcgggcc ggtgatctcg
      241 acccagcgct tgcgcagggc gggcggggcg ggggagacct gccagtcgcc gtcgcggatc
      301 tccttcgtct cctccaggaa gtcgagcgtg ccgcccgcgt ccagatcggc ctggacctca
      361 agccgcttgg ccagcagctc cttgcgccgg ccgttgatct cgcggtgcag cgcggccagc
      421 agctccagcg cctcgtcggt gagcacctcg tcgccgcgcg cgacgtccgg tccgttgatc
      481 tccactccgc tgacgctcat cggtctcctc tcagcgagcg ggccagcaac tcgaccgggt
      541 ggatcaccgg gagctccttg cccagctcgc gtgtgtgtgc ggttatctga agcgtgcagc
      601 cggggttggc cgccaccacg gcctcggccc cggtgtccag caggttctgt gccttgcgcc
      661 ggcccagctc ggccgccgcc tcgggcttga ccacgttgta gatgccggcc gatccgcagc
      721 acagctccca ctcggcgggc tcgaccagct cgacgccggg gatccctcgg agcaggtcgc
      781 ggggctgggt gcgcaccgcc tgggcgtggg ccaggtggca ggcgtcgtgg taggccacct
      841 tcatctcgat cgggttgcgg tgggcgcgcg gctcgccctc ggccaggatc tcggtcacgt
      901 cgaccacctt ctcggcgaag gcctgggcgc gttcggccca gtcggggtcc tggcgcatca
      961 cgtggccgta gtccttcatg gccgacccgc agccggcggc gttggccacg atccggtcgt
     1021 agccctccag cgcctcgatc gtcttcttca cgttggcggt ggcgtcggtg cccatgccgg
     1081 cgtgcagctg cagcgcgccg cagcaggacg ggtcctgcgg cgcctcgacg tcccagccct
     1141 cggcggccag cacggcggcg gtgtcggcgt tcacgccgct gaacatcacc cgctgcacgc
     1201 agccgcgcag cacggccacg gtgccgcgcg actcgccccg ggcggccacg tgctcgggca
     1261 gcgcgtgacg caccggcggg gccggggcca gcttggcgac ctcggcgatc cggcccagcg
     1321 gcagcgacgc ggtcagccgg tccagcttcg tggcggcgcc gaagcgcatc aggggcacca
     1381 gccagcgcat gcgctcgggg tgggggaaga gcgcgaacac gatcttgcgg gtccacttgt
     1441 cctggccgct gcgctcccac ttgcgctcga cctgggcgcg cgtgtcctcg atcagcttgt
     1501 cgtactgcac cccggacggg cacgcggtca cgcacgccat gcagcccagg cagttgtcga
     1561 agtggccgac catctcgccg gacagctcgg tctcctcctc gtggcccgcg cgcatcagca
     1621 cgatccggcc ccgcggcgag tcggcctctt cgccccacag ctggtaggtg gggcaggtgg
     1681 gcaggcagaa gccgcagtgc acgcagtcgt cgatcagcga cggctcgggc ggcttgtcct
     1741 cgtcccacat gccgatctgg atgggcccgg cctccatcag atcagccccg gggcgcacac
     1801 gccggcgggg tcgaagcgct cgcgcacgcg ctcgcccagc gccagcgcac cggggtcacg
     1861 cgggccccag gcgtccaggt cgccgcggcc gtccagcacg gcgcaggggg agggggagag
     1921 cgcgtcgcgc acacgctcgg cgtacccggg ctcggccacc gtgacccagg acagcccgta
     1981 ggcggcgcgg ccggccacgc gcgcgtcgag ctcctgcgcc agctccagca gcagcgcggt
     2041 ctggtcctgg aggccggaga cgcgcaccac ggtctgtgcc tcggaccgtt gcgcgtcgcg
     2101 ctgggcctgc cacagggcgc cgtcgtcgtc gtccacggag gcgtccagcc cctcggcggc
     2161 cagcaggccg gagacgtgct cggcggcctc ggccgccgcg gcaccgcccg agcgggccat
     2221 gaccgcgccg gagccgtcct cccaacccac gtccaggcac tccagctcca gccgcgcgtg
     2281 ggtcagcgcc gacgccgcgg cggccacggc gcgcgggtcg tcgcttcgcc ccaccgcggt
     2341 cacggtggcc ggcgccaggg gatgcaggcg aaccgcaacc tggaggatcg cgcccagagt
     2401 cccgtaggcg cccgcgaaca gcttggccag gtcgtagccg gccacgttct tgatcacctt
     2461 gccgccggac ttggccaccg tgccgtcgga cagggccacg gtcatgccca ccaccaggtc
     2521 gcgcacggag ccgtagcggt ggcgcagcgg cccggagtcg gcggtggcga tcacgccgcc
     2581 cacggtggcg cccaggtcgg gcgggtccag cgccagcatc tggccctcgg cggcgaacgc
     2641 ggcctgggcg tcggccagcc tcacgccggc gtccagaacg gcggtcaggt cgccgacgtt
     2701 gtgctcgacg atccggtcca gctccagggt ggagatctcc acgtcggccg gcgccgggcc
     2761 gccccagtcg aacttggtcc ggccgccggc gaagcgcacg gtggagcccg cctcggtcgc
     2821 atcgcgcagc ttctcgacga ccgcggcgct cacatccgct ccgcgatccc ggcttcctcg
     2881 agcgggtgct tgcggtacgg cccgggcacc tcaccgcaca ggcgcggggt gggcatgacc
     2941 ttgccggggt tggccaggcc gtcggggtcg aaggcgcagc gcaggcgctg gaacgtggcc
     3001 aggtcgtcct cgccgaacat ggagggcatg tagcgcttct tgtccacgcc cacgccgtgc
     3061 tcgccggtga tcgacccgcc ctcgtccacg cacgccttga cgatcatccc ggccagctcc
     3121 tcggcccgct cgggctcgcc ctcgaccgac ccgtcgtaac agaccagcgg gtgcaggttg
     3181 ccgtcgccgg cgtggaacac gttggccacc tggagctcgt actcggcgcc cagctcctcg
     3241 atccggcgca gcacggcggc catccgggtg cgcgggatca ccgagtcctg cacgtagtag
     3301 ttgggcgcga tccggcccat ggcggcgaag gcggccttgc gcgtcttcca gatcagcgcc
     3361 cgctcctcct cgtcctgcgc cacgcgcacc tgccacgagc cgccgtcctc gcacatcttg
     3421 accacggcgt cgaagcgggc ctcgcactcg tgctcggagc cgtccagctc gaccaccagc
     3481 gcggcccccg cgtcctgcgg atagccggcg ttggtggctc cctcggcggc cttgatcgac
     3541 aggttgtcca tcatctcgat ggcgccgggc acgatgccct cggagaccat ggccgagacc
     3601 acctcgcccg cctctccggt cgaatcgaag aaggccacga gcgtgcgcac cgcctccggc
     3661 gagggcacca cccgcagcgt gatcttggtg gccacgccca gcgtgccctc ggagcccacg
     3721 aacgcgccca ccaggtccag gccggggatg tcggcgccct tgccgccgag ctgcaccatc
     3781 tgcccgtcgg gcaggaccac ctccagcccg gtcacgtagt tggtggtgaa gccgtacttg
     3841 aagcagtgcg cgccgccgga gttctcggcc acgttgccgc cgatcgagca cacgatctgg
     3901 ctcgacgggt cgggcgggta gaagtggccc gggccgacgg cctcggacac ggcgatgttg
     3961 gtcacgccgg gctcgaccac cacgcgctgg ttggccaggt cgacctccag gatccggcgc
     4021 atccgcgaca cggcgatgac caccccgtcc tcgaccggca tcgcgccgcc cgacagcccc
     4081 gaccccgagc cgcgcgccac ccacggcgcc tcgtgctcgt ggcaggcctt gacgcacgcc
     4141 tgcacctgct cggccgtctc ggggagcgcc acggcgctgg gcagcgcggc gtactgcagc
     4201 agcccgtccg actcgtacgt gcgcagctgg tggtggtggg tcaggacgtg ctcgtcaccg
     4261 cacgcggcgc gcagcttcgc gaccagggcc gggtcggcgc tcaaaccacc atccagccca
     4321 gtatcgaggt cgattgcagg tacacgagga tccccatcag cgccgtcatc accagcgacc
     4381 agaacagtac ccgccggaag atgtccccct ccttcccggc catcccgacg gccgccgccc
     4441 cgatggccag gttctggggc gagatcatct tgcccagcac gccgccggag ctgttggccg
     4501 cggccaacag cgtctggtcg atgccggcct ccttggcggc ctgcacctgg agcgcgccga
     4561 acagcgcgtt cgaggacgtg tccgacccgg tgatggccac gcccagccag ccgatcaccg
     4621 gcgacagcag cgccaggatg ccgcccgcgc cggcgatcca caggcccagg gtcgaggtca
     4681 tgccgctcag gttcatcacg taggccaggc ccagcacggc catgacggtc acgatcgcca
     4741 gcgtcagctg cttcagcgtc tccccgtagg tgcgcagcgc gtcgcggatg ggcaccttga
     4801 tggccagcac ggtcagcagg ccgcagaaca gcaccaccgt gccgggggtg tcccagaacg
     4861 agaacgcgaa cgtcacggcg ctggccgcct ccccgtcggg cgtgaccacg tccaggccgg
     4921 gccagtcgaa gccgttcacg gtcttgtcgt ccgtgtcgaa gccggtgccg cccgagagct
     4981 cgaacaggaa cttctcgacc gggtcgatca gcttcacgat cgcgaagacg gtgacgatga
     5041 tgatgtaggg cgcgaacgcc tcccagcgct cgcgccgggt gtccgtctcc t
//
LOCUS       DASUPW010000152         3578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1144385, whole genome shotgun sequence.
ACCESSION   DASUPW010000152 DASUPW010000000
VERSION     DASUPW010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144385"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VFY44_03460"
     CDS             complement(<1..764)
                     /locus_tag="VFY44_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5909524.1"
                     /translation="MPEPRHGIFLAPFDELSEPAVVAELAARAEQAGWDGFFVWDHVE
                     YREPVRAVADPWITLAAVAVATERVTIGPMVTPLARRRPHQLARETVTLDRLSGGRLV
                     LGAGLGSPNTPEFRPERFGEEGDPKARAVLLDDGLEKLAAYWDGDFEPRPVQQPRIPV
                     WIASRWPSRRPLKRAARWDGMFPIEMPGPAELAELVAEAQSLRNVFAPFDVVVTHPAG
                     TESAPWVEAGATWCLSGFGPQPGRTEVEQAIDAGPS"
     gene            complement(769..1770)
                     /locus_tag="VFY44_03465"
     CDS             complement(769..1770)
                     /locus_tag="VFY44_03465"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5909525.1"
                     /translation="MARVFLTGGSGFIGGSLIERLHDRGDHVVGLARSDAAARVLSER
                     GAEVVRGNALDEDSMAAGMRGCDLAYHVAGLNSHCPKDPGLMMRVNVEGPQNAVRAAA
                     AAGIERVVLTSSAASVGEAQGTVGTEDSPHRGSYLSVYDRSKHEGELAAFAAAHHEEV
                     ELVAVNPSSVQGPGRTHGTGAIAIAYLNGKLPAFLDVPVSIVDIEDCADAHLLAAERG
                     RPGRRYVLNGATIPSTRALEIVSRLGGVEHKVRMIPPPVARAAAAASDGVYKLKEGTS
                     PLCAARVRTLLHGHRYDASRSERELGLTYTPVEETFARIVQWAVAEGLVEQPLAVAG"
     gene            complement(1773..2510)
                     /locus_tag="VFY44_03470"
     CDS             complement(1773..2510)
                     /locus_tag="VFY44_03470"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5909526.1"
                     /translation="MPPASELDERWSRTAVAARVREFLVCGVLGPCMDLYTRRTVSGR
                     DQLEHVDGPVVLVANHASHMDTPALLRALPGRRRRRTAVAAAVDYFYAKRRNAVAASL
                     VFGTVPVDRSGGGLSQDATGHLREWVDHGGTLLMFAEGTRTNDSKVGRLKSGAAVLAA
                     QQDVPLVPVYVAGTSEAMPRGHHWMVFRHRLFGERHPIEIRFGDPIRPRDGEEPAETM
                     ERVRLFLAESGAATEPDPRAVRAREPV"
     gene            complement(2564..>3578)
                     /locus_tag="VFY44_03475"
     CDS             complement(2564..>3578)
                     /locus_tag="VFY44_03475"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5909527.1"
                     /translation="SSGLQSDRYGDIADLTRGLRVVTPSGTLVVRPVPSTSTGPSVRE
                     MVLGSEGRLGLITEATVHVRRLAPDRQILGYLFPNWREGLAAMRDIAASEAEPSVTRV
                     SDAPETVFSFATRKASTPLDTVKSKALQLFLKRKGWDLEQMCLSFIGYEGTKQNVKIQ
                     RALTERIVKAHGGLGIGSSPGELYDQKKFDTPYIRDFLLDRGAPADVSETSAPWSELG
                     NVYDAVMEAGHGAFRELGVPGYLMCHMSHSYHAGACLYFTFAITPSGRRDSLEEYDTV
                     KNAIQQAFVDNGATLSHHHAVGTEHSRWLEQDISGPGVRMVEALLHGTDPGANLNPGK
                     IVK"
BASE COUNT          451 a         1326 c         1312 g          489 t
ORIGIN      
        1 taggagggcc cggcgtcgat ggcctgctcg acctcggtcc gcccgggctg gggcccgaag
       61 ccggacaggc accaggtggc gccggcctcg acccagggcg ccgactcggt gccggccggg
      121 tgggtgacca ccacgtcgaa gggcgcgaac acgttgcgca ggctctgggc ctcggccacc
      181 agctcggcca gctcggcggg accgggcatc tcgatcggga acatgccgtc ccagcgcgcg
      241 gcgcgcttca gcggacggcg gctgggccag cgtgaggcga tccacaccgg gatccggggc
      301 tgctgcaccg gccgtggctc gaagtcgccg tcccagtagg cggccagctt ctccaggccg
      361 tcgtccagga gcacggcgcg cgccttgggg tcgccctcct cgccgaagcg ctcgggccgg
      421 aactcgggcg tgttgggcga tcccagcccc gcgcccagga ccagccggcc gccgctgagg
      481 cggtccagcg tcaccgtctc gcgggccagc tggtgcggtc ggcgccgggc cagcggggtg
      541 accatgggcc cgatggtcac gcgctcggtg gccacggcga cggcggccag cgtgatccac
      601 ggatcggcca ccgcgcgcac gggctcgcgg tactcgacgt ggtcccagac gaagaagccg
      661 tcccagcccg cctgctcggc ccgcgccgcc agctcggcca cgacggccgg ctcgctgagc
      721 tcgtcgaacg gggccaggaa gatcccgtgg cgtggctcgg gcatcgcgtc agcctgccac
      781 ggccaggggc tgctccacaa gcccctcggc caccgcccac tgcacgatcc gggcgaaggt
      841 ctcctcgacc ggggtgtagg tcaggcccag ctcgcgctcc gagcgcgacg cgtcgtagcg
      901 gtgcccgtgc agcagggtgc gcacgcgcgc cgcgcacagc ggcgaggtgc cctccttgag
      961 cttgtacacg ccgtcgctgg cggcggccgc ggcgcgggcc acgggcggcg ggatcatccg
     1021 caccttgtgc tcgaccccgc ccagccggct gacgatctcc agcgcccggg tcgacgggat
     1081 cgtggcgccg ttgagcacgt agcgccgccc cgggcgcccg cgctcggcgg ccagcaggtg
     1141 ggcgtcggcg cagtcctcga tgtccacgat gctgaccggc acgtccagga aggcggggag
     1201 cttgccgttc aggtaggcga tggcgatggc tccggtgccg tgggtgcgcc cgggcccctg
     1261 caccgaggac gggttcaccg ccaccagctc gacctcctcg tggtgggcgg cggcgaaggc
     1321 cgccagctcg ccctcgtgct tggaccggtc gtagaccgac aggtatgagc cgcggtgggg
     1381 cgagtcctcg gtgcccaccg tgccctgcgc ctcgcccacc gaggccgccg acgaggtcag
     1441 caccacgcgc tcgatcccgg ccgccgcggc ggcccggacc gcgttctggg gcccctccac
     1501 gttcacgcgc atcatcaatc cggggtcctt cgggcagtgg ctgttcaggc cggccacgtg
     1561 gtaggccagg tcgcagccgc gcatcccggc ggccatggag tcctcgtcca gcgcgttgcc
     1621 gcgcaccacc tcggcgccgc gctcggacag cacgcgcgcc gcggcgtccg agcgggccag
     1681 ccccacgacg tggtcgccgc ggtcgtgcag gcgctcgatc agcgaccccc cgatgaagcc
     1741 gctgccgccg gtcaggaaga cccgtgccac ggtcagacgg gctcgcgcgc gcggaccgcg
     1801 cgcgggtcgg gctcggtggc cgcccccgac tcggccagga acaggcgcac gcgctccatg
     1861 gtctcggccg gctcctcgcc gtcccggggg cggatcgggt cgccgaagcg gatctcgatc
     1921 gggtggcgct cgccgaacag gcggtggcgg aagaccatcc agtggtgccc gcgcggcatc
     1981 gcctcgctgg tgccggccac gtagacgggc accagcggca cgtcctgctg ggccgccagc
     2041 acggcggcgc ccgacttcag gcgcccgacc ttgctgtcgt tggtgcgcgt gccctcggcg
     2101 aacatcagca gcgttccccc gtggtcgacc cactcgcgca ggtggccggt cgcgtcctgg
     2161 ctgagcccgc ccccgctgcg gtcgacgggc acggtgccga acaccagcga ggccgccacc
     2221 gcgttccggc gcttggcgta gaagtagtcc accgcggcgg cgacggccgt gcgccgccgg
     2281 cgcctgcccg gcagggcccg cagcagcgcc ggggtgtcca tgtggctggc gtggttggcc
     2341 accagcacca ccgggccgtc cacgtgctcg agctggtcac gcccgctgac cgtgcggcgc
     2401 gtgtacaggt ccatgcacgg gcccagcacg ccgcagacca ggaactcgcg cacgcgcgcc
     2461 gccacggcgg tgcgcgacca gcgctcgtcc agctccgacg cggggggcat acctctagta
     2521 ctccccggcc cggggccggt ttccacctgt tttcggttct gggctacttg acgatcttgc
     2581 ccgggttcag gttcgccccc gggtcggtcc cgtgcagcag cgcctccacc atccgcacgc
     2641 ccggcccgga gatgtcctgc tccagccagc gcgagtgctc ggtgcccacc gcgtggtggt
     2701 gcgagagcgt ggcgccgttg tccacgaagg cctgctggat cgcgttcttg accgtgtcgt
     2761 actcctccag cgagtcgcgc cggcccgacg gcgtgatggc gaacgtgaag tacaggcagg
     2821 cgcccgcgtg gtagctgtgg gacatgtggc acatcaggta gccgggcacg cccagctccc
     2881 tgaacgcccc gtgccccgcc tccatcacgg cgtcgtagac gttgcccagc tcgctccacg
     2941 gcgccgaggt ctcggacacg tcggcggggg cgccgcggtc cagcaggaag tcgcggatgt
     3001 agggggtgtc gaacttcttc tggtcgtaca gctcgcccgg gctggagccg atgcccaggc
     3061 cgccgtgggc cttgacgatc cgctcggtca gcgcgcgctg gatcttcacg ttctgcttgg
     3121 tgccctcgta gccgatgaac gagaggcaca tctgctccag gtcccacccc ttgcgcttga
     3181 ggaacagctg cagggccttc gacttgaccg tgtccagggg cgtcgaggcc ttgcgggtgg
     3241 cgaagctgaa cacggtctcg ggggcgtcgg acacgcgcgt gaccgagggc tcggcctcgc
     3301 tggcggcgat gtcgcgcatg gcggccaggc cctcgcgcca gttggggaac aggtagccca
     3361 ggatctggcg gtcgggcgcc agccggcgca cgtgcacggt cgcctcggtg atcaggccca
     3421 ggcgaccctc gctgcccagc accatctcgc gcacgctggg gccggtcgag gtggagggca
     3481 cagggcggac caccagcgtc ccggagggcg tgaccacccg caggccgcgc gtcaggtcgg
     3541 cgatgtcgcc gtaacggtcg gactgcaggc ccgacgag
//
LOCUS       DASUPW010000153         4760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1005860, whole genome shotgun sequence.
ACCESSION   DASUPW010000153 DASUPW010000000
VERSION     DASUPW010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005860"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..1239
                     /locus_tag="VFY44_03480"
     CDS             106..1239
                     /locus_tag="VFY44_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909528.1"
                     /translation="MAALPSAAHAAPGWSDLAVGSATTTSGAAVAGRSLAITTVTTNL
                     GSRGAYRAQTVVYLSRDGRRDPRDIAIGRGQVPALAPAARDTRMVSARVPNGLKGLFQ
                     VIACANADRPVRGARTRNNCRVVKHRLQAKPAPAPSPPEPSPAGSPPAPVLSPPGEEA
                     GPPAPPDTARLAVSASPRPFVLREVQSFTVTNTGQEPSAPVAISFAGADAAAYSVTAN
                     ACAGVHLAVGASCSVSARFVPPAGPGTGTATLNATAGAQQASTALSGQAFGTLRVNGG
                     VLNVLAEADLSSQAPTPVTIPIQNASLYPAPVAVRLNPSPVFVIAASTCGPDPLAPGA
                     SCAVELEAVNAPVGVTRGQLVVMNGPVTRVTVDILLSRLGSNF"
     gene            complement(1236..1490)
                     /locus_tag="VFY44_03485"
     CDS             complement(1236..1490)
                     /locus_tag="VFY44_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909529.1"
                     /translation="MPSDNFTEQRLAELEAKVDHMYRQLTGQGDIATVIPPMRREGAG
                     VSEAVREAARSGNMAEAIRLQRAETGQSLSEAKDAVTGAG"
     gene            complement(1632..1720)
                     /locus_tag="VFY44_03490"
     tRNA            complement(1632..1720)
                     /locus_tag="VFY44_03490"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1682..1684),aa:Ser,seq:tga)
     gene            1755..2420
                     /locus_tag="VFY44_03495"
     CDS             1755..2420
                     /locus_tag="VFY44_03495"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX5909530.1"
                     /translation="MDRISDRVHASLRSDILAGTLAPGDAVPSERRLAEDLGVNRHAV
                     REALKRLEQAGLITISQGGATRVRDWRDTGGLEILLDLQSGTEPPADLARSVLELRAS
                     VGVDAARRCAERADAEALARVEELAESTAAAMGQEGQAVDDGYAQLWRAVVLGSGNLA
                     YRLALNSLMAALAAYPEVAGVVRPAEPKKVRALGRAIAAADPAKAERAARALLEPDID
                     AFG"
     gene            2722..3177
                     /locus_tag="VFY44_03500"
                     /pseudo
     CDS             2722..3177
                     /locus_tag="VFY44_03500"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
     gene            complement(3360..4544)
                     /locus_tag="VFY44_03505"
     CDS             complement(3360..4544)
                     /locus_tag="VFY44_03505"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX5909531.1"
                     /translation="MLEVDGQSFLTIVVGAARAATTAGLMAGRLPVPVVVLEVVLGIM
                     IGPQLLGLAEPDQFVDFFASLGLGMLFFFAGYEIDFGRLKGEPIKLALLGWGLSLVLA
                     FTIGGLLAWAGVVLSLALTGAAMTTTAVGTLIPILDDAGELRTRFGTYLLGAGAVGEF
                     GPILMITLFLSTGRPLASALILVAFVIVSVLVALVAVRSMGTGWKLVEQTMEGSGQLG
                     VRLAVVLVFLLAFLAAELGLDLLLGGFMAGAIVRLALQGREVQVFESKLKAVGYGFFI
                     PFFFVVSGVKLNVEALAEDPVQLLKLPLFLACFLVVRGAPALLLYRKALAGRDRLSLA
                     FFSATGLPLIVAITTVGVERGEMRSSTAASLVAAGILSTSIFPLVGLKLRGDRSAAAA
                     AP"
BASE COUNT          697 a         1788 c         1649 g          626 t
ORIGIN      
        1 cggctcgagc ccgcgggcta gcggctcaag gcaggcctcc ttcgggccga tgagacgagg
       61 atgggcggtc gccggcgact ctcggtggct ctcgccctcc taggcatggc cgcccttccg
      121 tcggccgccc acgccgcgcc cggctggtcg gacctggccg tcgggtcggc cacgaccacc
      181 tccggcgccg ccgtggcggg cagaagcctc gccatcacca cggtcacgac caacctcgga
      241 tcgcgtggcg cctaccgcgc gcagacggtc gtctacctct cccgcgatgg ccgccgtgac
      301 ccccgcgaca tcgcgatcgg ccgcggccag gtccctgcgc tcgcgccggc cgcccgtgac
      361 acccggatgg tgtcggcgcg cgtgccgaac gggctcaagg gcttgttcca ggtgatcgcg
      421 tgcgccaacg ccgaccggcc cgtgcgcggc gccaggacgc ggaacaactg tcgggtcgtg
      481 aagcaccggt tgcaggcgaa gcccgctccg gcgccgtcgc ccccggagcc ctcacctgcc
      541 ggctcgccac cggcccctgt cctctccccg ccgggggaag aggccggacc ccccgccccg
      601 cccgacaccg cccggctggc ggtgagcgcg tctccccgcc ccttcgttct ccgggaggtc
      661 cagtcgttca ccgtcaccaa caccggccag gagccttctg cgccggtggc gatctccttc
      721 gcgggcgcgg acgccgccgc ctactccgtc accgcgaacg cgtgcgccgg ggtccatctc
      781 gccgtcggcg cgagctgctc ggtctcggcg cgcttcgtcc cgccggcggg acccggcacg
      841 ggcacggcga cgctgaacgc gaccgcgggt gcgcaacagg cgtcgaccgc cctctcaggc
      901 caggcgttcg gcacgttgcg cgtgaacggc ggtgtgctga atgtgctcgc tgaggctgac
      961 ctctcgtccc aggcccccac ccctgtgacc atcccgatcc agaacgccag cctgtatccc
     1021 gctcccgtgg cggtccggtt gaacccctct ccggtattcg tgatcgccgc cagcacgtgc
     1081 gggcccgacc cgctggcgcc cggtgcgagc tgcgccgtag agctcgaggc cgtcaacgcg
     1141 ccggtcgggg tcacgagggg tcagctggtc gtgatgaacg gccccgtcac ccgcgtcacg
     1201 gtcgacatcc tcctctcccg gctcgggagc aacttctagc ccgccccggt caccgcgtcc
     1261 ttcgcctcgc tcaggctctg accggtctcg gcgcgctgga gcctgatggc ctcggccatg
     1321 ttgccgctcc gggccgcctc gcgcacggcc tcggacacgc cggcgccctc gcgccgcatc
     1381 ggcgggatca cggtcgcgat gtctccctgg ccggtgagct ggcggtacat gtggtcgacc
     1441 ttggcctcga gctcggccag gcgctgctct gtgaagttgt cggaaggcat gggggcaacc
     1501 taaccggagc acgcgtgtgc cgtgcagctc gcgggttctg gagcgtcctg gcgcggaaga
     1561 ggccggcgaa gtgtgctccg cacacgagcc gtgccgatga caaagccagg gcacaccaga
     1621 acctgcgagg acggagcggg agggattcga acccccggac cacttgcgtg gtcggctgct
     1681 ttcaaggcag ctgcaataaa ccggactctg ccaccgctcc aacggtcgcg acggcgacac
     1741 gatagaaccg cagcgtggac cgcatctccg accgcgtgca cgcgtcgctg cgcagcgaca
     1801 tcctggccgg cacgctcgcg cccggtgacg cggtcccgtc cgagcgccgg ctggccgagg
     1861 acctgggcgt caaccgccac gcggtccgtg aggcactcaa gcgcctggag caggccggcc
     1921 tgatcaccat cagccagggc ggcgccaccc gcgtgcgcga ctggcgcgac accggcggcc
     1981 tggagatcct gctggacctc cagtcgggga ccgagccgcc ggcggacctg gcgcggtcgg
     2041 tcctggagct gcgggccagc gtgggggtgg acgcggcgcg gcggtgcgcc gagcgggcgg
     2101 acgccgaggc gctcgcgcgc gtggaggagc tggcggagag cacggcggcg gccatgggcc
     2161 aggagggcca ggccgtggac gacggctacg cccagctgtg gcgcgcggtg gtgctcggct
     2221 cgggcaacct ggcctaccgg ctggcgctga actcgctgat ggccgcgctg gccgcctacc
     2281 ccgaggtcgc gggggtcgtc cggccggcgg agccgaagaa ggtccgcgcc ctgggccgcg
     2341 cgatcgcggc ggcggacccg gcgaaggccg agcgcgccgc gcgcgccctg ctcgagccgg
     2401 acatcgacgc cttcggctga gccccgggcg cggggccccg agcgggccgg tcgccggcgg
     2461 ccgccggttg acagaccagc cggccaactg gtacaaccag tactggttag accagtcacg
     2521 accgctccgg aggcctcgcc cgtggaaaag ctcatcctca ccgcgatccc gttcttcttc
     2581 gtctcgatgg cgctggaggc gctggcgctc cgccacgccg cgcacgagaa cgagccgaag
     2641 ggctacgagc gcagcgacac ccgcaccagc ctggccatgg gcatcggcca cctgggcatc
     2701 ggggcggcgt ggaagctggt catgaccgcg atctacgcgg ccgtctacgt ggtcagcccg
     2761 ctgaagctgc ccaccgaggc gtggtggacc tgggtgatcc tcttcttcgc cgacgacctg
     2821 gcctactacg cctaccaccg cggccaccac cggatccggc tgttctgggc cacccacgtg
     2881 gtccaccaca gctcccagca ctacaacctg tcgaccgcgc tgcgccagga ctggacgccg
     2941 ttcagcgcca cgctgttctg gctcccgtcg gcgctgttct tcccgccgtg gatgatcttc
     3001 ctggcgctgg cctggaacct gctctaccag ttcacgctgc acacggaggc gatcgaccgc
     3061 tacccgcgcc cgatcgaaat ggtgttcaac acgcccagcc accaccgggt gcaccacggc
     3121 tcccaggagc agtacctgga ccgcaactac gcggggatcc tgatcatctg ggaccggctg
     3181 ttcggcacct tcgagcccga gggcgagcgg gtgcggtacg gcctgacgaa gaacatcgag
     3241 acgttcaacc ccgtgaaggt ggccttccac gagttcggct cgctgtggcg ggacgtccgc
     3301 gatgccccca cctggcgggc gcggatgggc tacctgttcc gcggcccggg gtgggagcct
     3361 cagggcgcgg cggcggccgc cgaccggtcg ccccgcagct tgagcccgac cagcgggaag
     3421 atgctggtgg acaggatgcc ggcggccacc agcgacgccg cggtggacga gcgcatctcg
     3481 ccgcgctcga cccccacggt ggtgatggcc acgatgagcg ggagcccggt ggccgagaag
     3541 aacgccagcg acagccggtc gcggcccgcc agcgccttgc ggtagagaag cagcgcgggc
     3601 gcgccgcgca ccaccaggaa gcaggccagg aacagcggca gcttcagcag ctgcaccgga
     3661 tcctcggcca gcgcctccac gttcagcttg acgccgctga ccacgaagaa gaacgggatg
     3721 aagaacccgt agccgacggc cttgagcttg gactcgaaca cctggacctc gcggccctgg
     3781 agcgccaggc gcacgatggc tccggccatg aagccgccca gcagcaggtc caggcccagc
     3841 tccgcggcca ggaaggccag caggaacacc agcaccacgg ccagccggac ccccagctgg
     3901 ccgctgccct ccatggtctg ctccaccagc ttccagccgg tgcccatcga gcgcaccgcc
     3961 accagcgcca ccagcacgct cacgatcacg aacgcgacca ggatcagcgc gctggccagc
     4021 ggccggcccg tggacaggaa gagggtgatc atcaggatcg gcccgaactc gcccaccgcg
     4081 ccggcgccca gcaggtaggt gccgaagcgg gtgcgcagct ccccggcgtc gtccaggatc
     4141 gggatcagcg tgcccacggc ggtcgtggtc atggctgcgc cggtcagcgc cagcgacagc
     4201 acgaccccgg cccaggccag cagcccgccg atggtgaagg ccaggaccag cgacaggccc
     4261 cagcccagca gcgcgagctt gatcggctcg cccttgagcc ggccgaagtc gatctcgtag
     4321 ccggcgaaga agaacagcat gcccaggccc aggctggcga agaagtccac gaactggtcc
     4381 ggctcggcca gcccgagcag ctgggggccg atcatgatgc ccaggaccac ctcgagcacc
     4441 accaccggca cggggaggcg ccccgccatg aggcccgcgg tggttgcggc acgggccgca
     4501 cccacgacga tcgtcaggaa cgactggccg tcgacctcga gcatgccgcg aggaacctat
     4561 ccgccccctc gcctggcgga taggcctgcg gtggcgtcag ccggggagct tgccgcggcc
     4621 cttgcggccg gcggaggcgc tcttgttggt cgccttgcgc gacttgccga acggcttctg
     4681 tgcccagcgt cccaggaatc cgggcaggac cccgccggcc ttctggcccg cgctcagggt
     4741 gctgtccacg ccgcgctgcg
//
LOCUS       DASUPW010000154         4501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_728771, whole genome shotgun sequence.
ACCESSION   DASUPW010000154 DASUPW010000000
VERSION     DASUPW010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728771"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1075
                     /locus_tag="VFY44_03510"
     CDS             167..1075
                     /locus_tag="VFY44_03510"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5909532.1"
                     /translation="MERADLPTGVSIAFDHEGAGGFPLLLIHGWPETRRIWSRNVDAL
                     AEAGFEVVAPDLRGFGDSGLAPDERYDAAAHARDMQALLEHLGHERCVVSAGDLGGVV
                     AQDLSLRFEGLVERMVLFNTIAPLLPAEYEAAGLGPTPPVNPQTSDYFVRQSRDADGL
                     AAELDTPERRRAYVAQMYGPRLWGGPDAFTRDEIDHLAEPFGDAESFRASIANYEYVG
                     GARTAPETPRMLELNPTPTLVLYGPEDHVIPYDFPRRMEVAFPEIVGPFVVAGAGHFL
                     MWEAARAFNRATRYFCLDLLTQSPAR"
     gene            1072..1710
                     /locus_tag="VFY44_03515"
     CDS             1072..1710
                     /locus_tag="VFY44_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909533.1"
                     /translation="MSASSSRAAELAAQQVEQIVAAAQEAADQIRAEAEGVRKEAREQ
                     GKRDAQRIRQKAKDESEATLMESRKQAVMMGQDARQEAEKAIKDAQEESERVREQTRR
                     AVEGRVAGAERAAAEVLKEAQALSGGQRQLGRSLEEQADRILRDVQAAHRRMQADLRV
                     QSGDELPTSSSSGRGTRPSAEPEPPPREASPRRRDGSSNPFDDLEVPGWTRK"
     gene            complement(1716..2777)
                     /locus_tag="VFY44_03520"
     CDS             complement(1716..2777)
                     /locus_tag="VFY44_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5909534.1"
                     /translation="MLELDVGARLGDVDLDARLAVAAGECLALAGPSGAGKSSLLRAA
                     AGLLRPDRGRVRCGERTWLDTDAGIDLAPERRRVGFLFQDYALFGHLSAWRNVAYGIG
                     AGPRAQRRRRAHELLDRFGLAARADARPATLSGGERQRVALARALAREPEVLLLDEPL
                     SALDAATRARAGRELAALIAEAGVPVLMVTHDFGEAALLADRVAVLDRGAVVQTGTAT
                     ELAAAPASAFVADLTGAVVLTGTAHAEGGLTAVDLDGGGRVVSTDAASGPVAVTVHPW
                     EISVEHAGPGAGGSARNRLPATVTSVTTVGNRVRVGLLGSQPMVAELTGEAAAGLGLS
                     TGVGAVAAFKATATRLVPR"
     gene            complement(2764..3567)
                     /locus_tag="VFY44_03525"
     CDS             complement(2764..3567)
                     /locus_tag="VFY44_03525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5909535.1"
                     /translation="MTRGTAFTGLLVAALTVVLAFLTLPLVAIFLDVGPGDLVDSLGD
                     EGSVDALLLSLEVTLIALVLIVVLGTPAAWLLATRSFPGRSVAITLVELPLVVPPAVA
                     GIGLLAALGPEGLLGGTLEAAGVELVFQTAGVVVALVFVASPFYIRQALAAFGALDPG
                     VIEASRTLGAGDGRTFLRVALPGARAGLVAGGALAWGRALGEFGATLVFAGSLSGVTQ
                     TAPLAIYERFSDDFTGALALSAVLVAASGAILLAVKPAGGARVLGDARA"
     gene            complement(3564..4337)
                     /gene="modA"
                     /locus_tag="VFY44_03530"
     CDS             complement(3564..4337)
                     /gene="modA"
                     /locus_tag="VFY44_03530"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5909536.1"
                     /translation="MRRAPLLLLAALALALAGCGDDGGSGSGGDAEPRLTVSAAASLK
                     GAFEAYGEEFPDATARFSFAGSDELAAQIRKGVKPDVLAAANTKLPDQLHAEGRVGKP
                     TIFAGNRLVLAVPADSARVGSLDDLAEPGVELAVGAESVPVGSYTREVLGRLPTGEEK
                     AILANVRSNEPDVKGVVGKLTQGAADAGFVYVTDVVATEGKVKAIELPASLQPSVEYG
                     VAVVKGAKEPEAARAFIEGLTDGAGARALDDAGFEPPPK"
BASE COUNT          609 a         1768 c         1630 g          494 t
ORIGIN      
        1 cccgaggggt agggcagcat ctcgagcacg tagctcttgg ggcgcccgtc gtcctcgttg
       61 gacacctccc aggtgcgctc gtcggcccac acctgctgcc agcggggctc gatctgcctg
      121 gggtcgtagc ggtcgtccac ctgcggaaga atagggtcgg cagatcgtgg agcgggctga
      181 ccttccaacc ggtgtctcga tcgcgttcga tcacgagggc gcgggcggct tcccgctgct
      241 cctgatccac ggctggccgg agacgcgccg gatctggtcg cgcaacgtgg acgcgctggc
      301 cgaggccggc ttcgaggtgg tcgcgccgga cctgcgcggg ttcggcgact cgggcctcgc
      361 ccccgacgag cgttacgacg cggcggccca cgctcgggac atgcaggcgc tgctcgagca
      421 cctggggcac gagcgctgcg tggttagcgc gggcgacctg ggcggcgtgg tggcgcagga
      481 cctgtcgctg cgcttcgagg gcctggtcga gcggatggtc ctgttcaaca ccatcgcccc
      541 cctcctcccg gccgagtacg aggccgcggg gctgggcccg accccgccgg tgaacccgca
      601 gacctccgac tacttcgtgc gccagtccag ggacgccgac gggctggcgg ccgagctgga
      661 cacgccggag cgccggcgcg cgtacgtggc ccagatgtac gggccgcggc tgtggggcgg
      721 cccggacgcg ttcacgcgcg acgagatcga ccacctggcc gagccgttcg gcgacgcgga
      781 gtcgttccgg gcctcgatcg ccaactacga gtacgtgggc ggcgcccgga ccgcgccgga
      841 gacgccgcgg atgctggagc tcaacccgac ccccacgctg gtcctctacg gccccgagga
      901 ccacgtgatc ccgtacgact tcccgcgccg gatggaggtc gcgttccccg agatcgtcgg
      961 gcccttcgtg gtggcgggcg cggggcactt cctgatgtgg gaggcggccc gtgcgttcaa
     1021 ccgtgcaacg cggtacttct gcctggacct cctgacacaa tcacctgcac gatgagcgct
     1081 tccagctcac gcgcggccga gctggccgcc cagcaggtag agcagatcgt ggccgccgcc
     1141 caggaggccg cggaccagat ccgcgccgag gcggagggcg tgcgcaagga ggcgcgcgag
     1201 cagggcaagc gcgacgccca gcgcatccgc cagaaggcca aggacgagtc cgaggcgacg
     1261 ctgatggaat cgcgcaagca ggccgtgatg atgggccagg acgcccgcca ggaggccgag
     1321 aaggcgatca aggacgcgca ggaggagtcc gagcgggtgc gcgagcagac ccgccgcgcc
     1381 gtggagggcc gcgtggccgg cgccgagcgc gccgccgccg aggtgctcaa ggaggcgcag
     1441 gcgctgtccg gcggccagcg ccagctcggg cgctcgctcg aggagcaggc cgaccggatc
     1501 ctgcgcgacg tgcaggccgc ccaccggcgc atgcaggccg acctgcgcgt gcagtccggc
     1561 gacgagctcc cgacgtcctc ctcgtcgggc cgcgggaccc ggcccagcgc cgagcccgag
     1621 ccgccgccgc gggaggcatc cccccgccgt cgcgacgggt cgtcgaaccc gttcgacgac
     1681 ctcgaagtgc cgggctggac gcggaagtag ccgcgctagc gcggcacgag ccgcgtcgcg
     1741 gtggccttga acgccgccac cgccccgacc ccggtggaca ggccgagccc tgccgccgcc
     1801 tcgccggtca gctcggcgac catcggctgc gagcccagga gccccacgcg cacgcggttg
     1861 ccgacggtgg tcacggaggt caccgtggcc ggcagccggt tgcgcgccga cccgccggcg
     1921 ccgggccccg cgtgctccac gctgatctcc cacgggtgca cggtcacggc caccgggccc
     1981 gaggcggcat cggtgctgac cacgcggccg ccaccgtcca ggtccaccgc ggtcagcccg
     2041 ccctcggcgt gcgccgtgcc ggtcagcacc acggcgcccg tcaggtcggc cacgaacgcc
     2101 gacgcgggcg ccgcggccag ctcggtggcg gtcccggtct gcaccaccgc accgcggtcg
     2161 agcacggcca cgcggtcggc cagcagcgcc gcctcgccga agtcgtgggt caccatcagc
     2221 accggtacgc cggcctcggc gatcagggcc gccagctccc ggccggcccg ggcgcgggtg
     2281 gcggcgtcca gcgccgacag cggctcgtcc agcagcagca cctcgggctc gcgcgccagc
     2341 gcccgcgcca gggccacgcg ctggcgctca cccccggaca gggtcgcggg ccgggcgtcc
     2401 gcgcgggcgg ccaggccgaa ccggtccagc agctcgtggg cccggcggcg ccgctgcgcg
     2461 cgcgggccgg ccccgatgcc gtaggccacg ttgcgccagg cgctcaggtg cccgaacagc
     2521 gcgtagtcct ggaacaggaa gccgacccgc cggcgctcgg gggccaggtc gatccccgcg
     2581 tccgtgtcca gccaggtgcg ctcgccgcag cgcacccgcc cgcggtcggg gcgcagcagg
     2641 ccggccgccg cccgcagcag cgagctcttg cccgcgcccg acgggccggc cagcgccagg
     2701 cactcgccgg cggccaccgc caggcgcgcg tccaggtcga cgtcgcccag ccgggcgccg
     2761 acgtcaagct cgagcatcgc ccagcacccg cgcgccgccg gccggcttga cggccaggag
     2821 gatcgcgccg ctggccgcca caagcaccgc cgacagcgcc agcgcgccgg tgaagtcgtc
     2881 cgagaagcgc tcgtagatgg ccagcggggc cgtctgggtg acgccgctca ggctgccggc
     2941 gaagaccagc gtggccccga actcgcccag cgcccggccc caggccagcg ccccaccggc
     3001 caccaggccg gcgcgcgccc ccggcagggc gacgcgcagg aacgtgcgcc cgtcgcccgc
     3061 ccccagcgtg cgcgaggcct cgatgacccc cgggtccagt gcgccgaacg ccgccagcgc
     3121 ctggcggatg tagaacgggc tggccacgaa caccagcgcc accaccacgc ccgcggtctg
     3181 gaacaccagc tcgacgcccg ctgcctcgag cgtgccgccc aggaggccct cgggccccag
     3241 tgcggccagc aggccgatgc cggccaccgc gggcggcacc accagcggca gctcgaccag
     3301 ggtgatggcc accgaccggc ccgggaacga gcgggtggcc agcagccacg cggcgggtgt
     3361 gcccagcacc acgatcagca ccagcgcgat cagcgtgacc tccaggctca ggagcagcgc
     3421 gtcgaccgac ccctcgtcgc ccaggctgtc caccaggtcg cccggcccga cgtccaggaa
     3481 gatcgccacc agcggcagcg tcaggaacgc cagcaccacg gtcagggcgg cgaccaggag
     3541 cccggtgaag gccgtcccgc gagtcacttc ggcggcggct cgaaccccgc gtcgtccagc
     3601 gcccgcgcgc cggccccgtc ggtcagcccc tcgatgaacg ccctggccgc ctcgggctcc
     3661 ttcgcgccct tcaccacggc cacgccgtac tccacgctcg gctgcagcga cgccggaagc
     3721 tcgatggcct tgaccttgcc ctcggtggcc acgacgtcgg tcacgtacac gaagccggcg
     3781 tcggcggcgc cctgggtgag cttgcccacc acccccttca cgtccggctc gttcgagcgc
     3841 acgttggcca ggatcgcctt ctcctcgccg gtgggcagac ggccgaggac ctcgcgggtg
     3901 taggacccga cgggcaccga ctcggccccc acggcgagct cgacgccggg ctcggccagg
     3961 tcgtccaggc tcccgaccct ggccgagtcc gccggcacgg ccagcaccag ccggttgccg
     4021 gcgaagatcg ttggcttgcc gaccctgccc tccgcgtgga gctggtcggg cagcttggtg
     4081 ttcgccgcgg cgaggacgtc gggcttgacg cccttgcgga tctgcgccgc cagctcgtcc
     4141 gagcccgcga acgagaaccg cgcggtcgcg tccgggaact cctcgccgta ggcctcgaac
     4201 gctcccttca gcgacgcggc ggccgacacg gtcagccgcg gctcggcgtc gccgccggag
     4261 ccgctgccgc cgtcgtcacc gcacccggcc agggcgagcg cgagcgcggc caggagcagg
     4321 agcggcgcgc gccgcatcac ccgctccccc gctcgaccat gaccgaggtg gccttcacgg
     4381 ccgcgatcgc aggcacgccg ggcgccaggc caagctcctc gaccgagtcg cgggtgacgg
     4441 ccgcgatcac gcggtgcggg ccggcctcga tctcgaccag ggccatgacc ccgtcgacct
     4501 c
//
LOCUS       DASUPW010000155         7030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1975920, whole genome shotgun sequence.
ACCESSION   DASUPW010000155 DASUPW010000000
VERSION     DASUPW010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7030
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1975920"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1541)
                     /locus_tag="VFY44_03535"
     CDS             complement(<1..1541)
                     /locus_tag="VFY44_03535"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5909537.1"
                     /translation="MEPTRNIAARAGRWSARHRKAAVLGWLAFVVAALVLGGATGTKL
                     LTDEEQGSGESGRADKALAKAFPQAADESVLVQSSKLDVDDPAFRAAVADVQRRLGRV
                     KGVEGVTSPYRSDAAIAKNRDAVLVNRKIPGDDDKAEEKIDSVLAATAAAQKAHPDLR
                     IAQFGDASADKAISKSFEDDFKKAEITSLPVTLLILVIAFGALAAAGVPLLLAISSVA
                     ATIGLIGPISQFAPVSESINSVILLIGLAVGVDYAMFYLRREREERAKGAGERAALEA
                     AAATSGRAVMISGFTVMIAMAGMYIAGDATFTSFATGTILVVAIAVLGSLTVLPAVLA
                     KLGDRVEKGRIPFLARLKSRDGESRVWNAVLDRVLRRPLAAALLSGALLVALAVPALG
                     LKTALPGVDTLPRDLTVMQTYDRIQAEFPGENIPATVVVQGDAVGSPQMKAAVASLQR
                     QVDRSPVLEGRVSTETSPGGNVTRIDVPIAGNGTDAKSRAALAELRDRAVPEAFGPLG
                     VKADVG"
     gene            complement(1606..2259)
                     /locus_tag="VFY44_03540"
     CDS             complement(1606..2259)
                     /locus_tag="VFY44_03540"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5909538.1"
                     /translation="MSDRAPGILARGPWAPEDVSVRWDDAPYAASPEADAAADATLAE
                     LAERGSPTHDGMAARMAGLDVGAGGVDLVCQPMRWSLRLGEDAADSLSALCVVRDGDG
                     RWLAGRRAAWVASWPGRWALGAGGSVEVGENPVDTLPRELHEEWSLVPDRLTVEALIR
                     IPSGMVMLVGLARVADASGLVMDPEHDEHAWWPPDPGDWPDEADPPLRLMATLLAGG"
     gene            complement(2256..2939)
                     /locus_tag="VFY44_03545"
     CDS             complement(2256..2939)
                     /locus_tag="VFY44_03545"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005851219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX5909539.1"
                     /translation="MVQHATEAEPAVPAPPRGPPPPARGGPLAFLRFAHRNAMLRPRY
                     ARLALTLLRRRLLTTYGRRLHTDGFAFIASDVKLQITPRGRVELGRWSWLGHGCKIRC
                     HEGLVSIGAKTVLGQECTISAYQHVSIGRECVIADRVMLIDFDHGMVEVERPIRLQGI
                     YKRDVRVGNNVWIGYGACILRGVTVGDNAVIGTNAVVTHDVPANAVVGGVPAKVIRMR
                     EEPRELRWE"
     gene            3021..3374
                     /locus_tag="VFY44_03550"
     CDS             3021..3374
                     /locus_tag="VFY44_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5909540.1"
                     /translation="MAATVLIVDDHPSFRQSARLLLESDGWEVVGEAADGHSALTAAQ
                     ELGPDLVLLDVQLPDLDGFEVARRLRSSPPAPSVVMTSSRDACDYGTLAVDSGARGFV
                     AKAELSGNALEALLR"
     gene            3371..5119
                     /locus_tag="VFY44_03555"
     CDS             3371..5119
                     /locus_tag="VFY44_03555"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX5909541.1"
                     /translation="MTGLRRALVGIGALALVVGGAVGAIIVSSDFTDVRGLVLAFALL
                     GSWGFVAAGLFVWWRRPDRKVGALMTLAGFSWLITSLTAAENPLIFSMAVALSNVFAA
                     VLMHLLLSFPDGELRTRGDRLTVWAGYGLTTVLVIPLYVLADPIDDCSDCPAYVLDAA
                     HVRLVEDVGWPVLNVIGVVVLSRVLWTVVHRWREASPPSRRVVGPVYLMGSLTLILLA
                     FALSVQLLSLPDAVGEVAFWLATTVFVLLPYVFLGGLLRSRVLQAGAVGRLMARLTET
                     GPGPGNLGHELAVALGDQTLTLAFWLPERGRYVDSTGQEVELPPIGSGRTVTPVERDG
                     ERVAAIIHDASLTEDSGLVEAVGSAAALALENERLDAELRARLEDLRASRARLVEAGM
                     AERRRLERDLHDGAQQRLVSLALSLRLARNKVDTDPGGAVEVMDGAARELELALEELR
                     ELARGIHPAVLSDRGLDAALRTLAARAPLPVDLRTSVNGIPDAVESAAYFVVSEALTN
                     VAKYAQASSAVVQVEQDHGFVMVAVSDDGIGGADPSAGTGLRGLGDRLSALDGRLEVD
                     SAPERGTTVRARIPCA"
     gene            5110..5760
                     /locus_tag="VFY44_03560"
     CDS             5110..5760
                     /locus_tag="VFY44_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5909542.1"
                     /translation="MRVVVAEDSVLLREGVVRLLSEAGFDVVAQTGDADDLRRRVYAH
                     KPDVAIVDVRMPPDHTDDGLRAALEIRDRQPATGVLVLSQYIEEGYALDLITQAGVDG
                     GVGYLLKDRVADVDRFLDAVRRVAGGGSALDPEVVAQLLGRRRRDDPLEALTPREREV
                     LELMAEGRSNVAIADTLVVTERAVEKHVTSIFHKLELTPTAGDHRRVLAVLAFLQT"
     gene            5832..6560
                     /locus_tag="VFY44_03565"
     CDS             5832..6560
                     /locus_tag="VFY44_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5909543.1"
                     /translation="MTSEPIVTATDLHRRYGEGDAAVDALDGVTVSFERGRFAAIMGP
                     SGSGKSTLMHTLAGLDRPTSGSVVVDGVELADLDDDELTNLRRDKLGFIFQFFNLLPV
                     LSAEENIVLPLSIAGRDPDQEWLNRLIDVVGLGDRRTHRPAELSGGQQQRVAVARALV
                     SRPAVVFADEPTGNLDSTASAEVLRLLRQAVDEFGQTVVMVTHEAHAAAYADRLVVLR
                     DGKVAHDGEAGTEAQVMEHMKDAG"
     gene            6566..>7030
                     /locus_tag="VFY44_03570"
     CDS             6566..>7030
                     /locus_tag="VFY44_03570"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5909544.1"
                     /translation="MRKLILRGLAERRSRLAGVVLAVFLGVSLVAGSLVFTDTINASF
                     DKLFERSLKGSDVVVTPREVVRQDQDEPPPFSASLLSRVRDTDGVEAASGAIFAVVRL
                     VDDKNENLGAGFAPNFVSSTVPERFDPLTYTDGRAPRTGSEAAIDSGTAERSG"
BASE COUNT          895 a         2564 c         2593 g          978 t
ORIGIN      
        1 cccacatcgg ccttcacgcc cagcggcccg aacgcctcgg gaaccgcgcg atcgcgcagc
       61 tcggccagcg cggcacgcga cttggcgtcc gtgccgttcc cggcgatcgg cacgtcgatc
      121 cgggtcacgt tcccgccggg gctggtctcg gtgctgaccc tgccctccag gaccgggctc
      181 cggtcgacct gccgttgcag cgaggccacg gccgccttca tctgcggcga gcccacggcg
      241 tcgccctgca ccaccacggt ggcggggatg ttctcgcccg ggaactcggc ctggatgcgg
      301 tcgtaggtct gcatcacggt caggtcgcgc ggcagcgtgt ccacgcccgg cagggcggtc
      361 ttcagcccga gcgccgggac ggccagcgcc accagcaggg cgcctgacag cagcgcggcc
      421 gccagcggcc ggcgcagcac ccggtccagc accgcgttcc acacccgcga ctcgccgtca
      481 cgggacttca gccgtgcgag gaacgggatc cggcccttct cgacccggtc gcccagcttg
      541 gccaggaccg cgggcagcac ggtcagtgag cccagcacgg cgatcgcgac caccaggatc
      601 gtgccggtgg cgaacgaggt gaacgtggcg tcgccggcga tgtacatccc ggccatggcg
      661 atcatgaccg tgaacccgga gatcatgacc gcgcggcccg aggtggcggc cgcggcctcc
      721 agcgcggcgc gctcgccggc gcccttggcg cgttcctcgc gttcgcggcg caggtagaac
      781 atggcgtagt cgacgccgac ggccaggccg atcagcagga tcaccgagtt gatcgactcc
      841 gacaccggcg cgaactggct gatggggccg atcaggccga tggtggcggc caccgacgag
      901 atggccagca gcagcggcac gccggcggcg gccagcgccc cgaaggcgat gaccaggatc
      961 agcagcgtca cgggcagcga ggtgatctcg gccttcttga agtcgtcctc gaacgacttg
     1021 gagatggcct tgtcggcgct ggcgtcgccg aactgggcga tgcgcaggtc cggatgggcc
     1081 ttctgggccg cggccgtcgc ggccaggacc gagtcgatct tctcctcggc cttgtcgtca
     1141 tcgccgggga tcttccggtt gaccaagacc gcatcgcggt tcttggcgat cgccgcgtcg
     1201 ctgcggtacg gcgaggtcac gccctcgacg cccttcacgc ggccgagcct gcgctggacg
     1261 tccgcgaccg cggcgcggaa cgccgggtcg tccacgtcga gcttcgagct ctgcaccagc
     1321 acggactcgt cggcggcctg ggggaaggcc ttggccagcg ccttgtcggc gcggccggac
     1381 tcgcccgagc cctgttcctc gtcggtcagg agtttcgtgc ccgtggcccc accgagcacc
     1441 agtgcggcca cgacgaacgc cagccatccg aggacggcgg ccttgcggtg ccgggcgctc
     1501 cagcgcccgg cccgcgctgc gatgttccgt gtgggttcca tgccgcccag aatcgctccg
     1561 gggcggctcc gttcccatgg gggagcccgg cgtctgcggg tgtggttatc ccccggccag
     1621 cagtgtggcc atcaggcgta gaggtgggtc ggcctcgtcc ggccagtcgc ccgggtcggg
     1681 cggccaccac gcgtgctcgt cgtgctcggg gtccatgacc agccccgagg cgtcggccac
     1741 ccgcgccagg cccaccagca tcaccatgcc cgacgggatc cggatcagcg cctcgacggt
     1801 cagccggtcg ggcaccagcg accactcctc gtgcagctcg cgcgggaggg tgtcgaccgg
     1861 gttctcgccc acctcgaccg acccgccggc gcccagggcc caccgccccg gccagctggc
     1921 cacccaggcg gcgcgccgcc cggccagcca gcgcccgtcg ccgtcgcgca ccacgcacag
     1981 cgccgacagc gagtcggccg cgtcctcgcc caggcgcagc gaccagcgca tgggctggca
     2041 caccaggtcc acgccgcccg cgcccacgtc cagcccggcc atccgggcgg ccatcccgtc
     2101 gtgggtgggc gagccgcgct cggccagctc cgccagcgtg gcgtccgccg ccgcgtcggc
     2161 ctcgggcgag gcggcgtagg gggcgtcgtc ccagcgcacg ctgacgtcct ccggcgccca
     2221 cgggccccgg gccaggatgc ctggcgcccg gtcgctcatt cccagcgcag ctcccgcggc
     2281 tcctcgcgca tgcggatgac cttcgcgggc acgccgccga ccaccgcgtt ggcgggcacg
     2341 tcgtgggtga ccacggcgtt ggtgccgatc acggcgttgt cgccgacggt cacgccgcgc
     2401 aggatgcacg cgccgtagcc gatccagacg ttgttgccca cgcgcacgtc gcgcttgtag
     2461 atgccctgga ggcggatcgg gcgctccacc tcgaccatcc cgtggtcgaa gtcgatgagc
     2521 atcacgcggt cggcgatcac gcactcgcgc ccgatcgaca cgtgctggta ggccgagatc
     2581 gtgcactcct ggccgagcac ggtcttggcc ccgatggaca ccagcccctc gtggcagcgg
     2641 atcttgcagc cgtggcccag ccacgaccag cgccccagct ccacgcgccc gcggggcgtg
     2701 atctgcagct tcacgtcgga ggcgatgaag gcgaagccgt cggtgtgcag gcggcggccg
     2761 tacgtggtca gcagccgccg gcgcagcagg gtcagcgcca gccgggcgta gcgcgggcgc
     2821 agcatcgcgt tgcggtgggc gaagcgcagg aaggccagcg ggccgccgcg ggcgggtggg
     2881 ggagggccgc gcggcggtgc gggaacggcg ggctcggcct cggtggcgtg ctggaccatc
     2941 gcgggccgga tgataggcgc ggggctggtg cggctagccg tattggcgat tcgttgcggg
     3001 ctggcgacaa tcatgctgca gtggcagcca cggtgctcat agtcgacgac caccccagct
     3061 tccgccagag cgctcggttg ctactggaat cggacggctg ggaggtggtc ggtgaggccg
     3121 ccgacggcca ctccgcgctg accgcggcgc aggagctcgg ccccgatctc gtgctcctcg
     3181 acgtgcagct gccggacctg gacggcttcg aggtcgcccg ccgcctgcgc tcgagcccgc
     3241 ccgccccctc ggtggtcatg acctccagcc gcgacgcgtg cgactacggc accctggcgg
     3301 tggacagcgg tgcgcgcggg ttcgtggcca aggccgagct gtcgggcaac gccctcgagg
     3361 ccctcctgcg atgaccggcc tgcggcgggc gctggtcggc atcggcgcgc tggccctggt
     3421 ggtaggcggc gccgtcgggg cgatcatcgt cagcagcgac ttcacggacg tgcgcgggct
     3481 ggtgctggcc ttcgccctcc tgggcagctg gggcttcgtg gccgccgggc tgttcgtgtg
     3541 gtggcgccgt cccgaccgca aggtgggggc gctgatgacc ctggccggct tcagctggct
     3601 catcacctcg ctgaccgccg ccgagaaccc gctgatcttc tcgatggcgg tcgctctgag
     3661 caacgtgttc gcggccgtcc tgatgcacct gctgctgtcg ttccccgacg gcgagctgcg
     3721 cacccgcggc gaccggctga cggtgtgggc cgggtacggc ctgaccaccg tcctggtgat
     3781 cccgctgtac gtgctcgcgg acccgatcga cgactgctcg gactgcccgg cctacgtgct
     3841 cgacgccgcc cacgtccgcc tggtcgagga cgtgggatgg ccggtgctca acgtgatcgg
     3901 cgtggtcgtg ctctcgcggg tgctctggac agtggtccac cgctggcgcg aggccagccc
     3961 gcccagccgg cgcgtggtcg gcccggtcta cctgatgggc tcgctgacgc tgatcctgct
     4021 ggcgttcgcc ctgtcggtgc agctgctgag cctgcccgac gccgtcggcg aggtggcgtt
     4081 ctggctggcc acgacggtgt tcgtgctcct gccctacgtg ttcctgggcg ggctgctgcg
     4141 cagccgcgtg ctccaggccg gggcggtggg caggctgatg gcgcgcctga ccgagacggg
     4201 gcccgggccg ggcaacctcg gccacgagct ggcagtggcc ctgggcgacc agaccctgac
     4261 gctggccttc tggctgcccg agcgcgggcg ctacgtggac tccaccggcc aggaggtcga
     4321 gctcccaccg atcgggtcgg gccggaccgt cacgcccgtg gagcgcgacg gcgagcgggt
     4381 ggcggccatc atccacgacg cgtcgctgac cgaggactcg gggctggtgg aggccgtggg
     4441 cagcgcggcc gcgctggcgc tggagaacga gcggctcgac gccgagctgc gggcgcggct
     4501 ggaggacctg cgcgcgtccc gcgcgcggct ggtggaggcg ggcatggccg agcgccgccg
     4561 gctggagcgc gacctgcacg acggggcgca gcagcggctg gtgtcgctgg cgctgtcgct
     4621 gcggctggcg cgcaacaagg tggacaccga ccccggcggc gcggtcgagg tcatggacgg
     4681 cgccgcgcgc gagctggagc tggccctcga ggagctgcgc gagctggcgc ggggcatcca
     4741 cccggccgtg ctgagcgacc gcggcctgga cgccgcgctg cgcacgctgg ccgcccgcgc
     4801 gccgctcccg gtggacctga ggacgagcgt caacggcatc cccgacgccg tcgagtcggc
     4861 cgcctacttc gtggtctcag aggcgctgac caacgtggcc aagtacgccc aggccagctc
     4921 ggcggtggtc caggtcgagc aggaccacgg cttcgtgatg gtggcggtca gcgacgacgg
     4981 catcggcggc gccgacccca gcgccggcac cggccttcgc ggcctcggtg accgcctcag
     5041 cgcgctggat gggcggctcg aggtggacag cgcccccgag cgcggcacca ccgtcagggc
     5101 gaggatcccg tgcgcgtagt cgtggccgag gactcggtgc tgctccgcga gggggtggtc
     5161 cgcctgctgt ccgaggccgg cttcgacgtc gtggcccaga ccggagacgc cgacgacctg
     5221 cgccggcgcg tgtacgcgca caagcccgac gtggccatcg tcgacgtgcg catgccgccc
     5281 gaccacaccg acgacggcct gcgcgccgcg ctggagatcc gcgaccgcca gcccgccacc
     5341 ggcgtgctgg tgctgtccca gtacatcgag gaggggtacg cgctggacct gatcacccag
     5401 gccggggtcg acggcggcgt ggggtacctg ctcaaggacc gcgtcgccga cgtggaccgc
     5461 ttcctggacg ccgtccggcg ggtggccggc ggcggctcgg cgctggaccc cgaggtcgtc
     5521 gcccagctgc tgggccggcg ccgccgcgac gacccgctcg aggccctgac cccgcgcgag
     5581 cgcgaggtgc tggagctgat ggccgagggc cgctccaacg tggccatcgc cgacaccctg
     5641 gtggtcaccg agcgcgccgt cgagaagcac gtcacctcga tcttccacaa gctcgagctg
     5701 acgcccacgg ccggggacca ccggcgcgtg ctggccgtgc tggccttcct gcagacctag
     5761 gggacatccc tgagatacgg gtagggatat ccacacttgt gcccgctggg ccaactggga
     5821 cagtggtgcc catgaccagc gagcccatcg tcaccgccac cgacctgcac cgccgttacg
     5881 gggagggcga cgccgccgtg gacgccctcg acggcgtgac cgtgtcgttc gagcgcgggc
     5941 gcttcgcggc catcatgggc ccgtcgggct cgggcaagtc cacgctgatg cacaccctgg
     6001 ccgggctgga ccggcccacc tcgggctcgg tcgtggtcga cggcgtcgag ctggcggacc
     6061 tggacgacga cgagctcacg aacctgcgcc gggacaagct cggcttcatc ttccagttct
     6121 tcaacctgct gccggtcctg tcggcggagg agaacatcgt gctgccgctg tcgatcgccg
     6181 gccgcgaccc cgaccaggag tggctgaacc ggctgatcga cgtcgtgggc ctgggcgacc
     6241 gccgcaccca ccggccggcc gagctctcgg gcggccagca gcagcgcgtg gccgtggccc
     6301 gggcgctggt ctcgcgcccg gcggtggtgt tcgccgacga gcccaccggc aacctggact
     6361 cgaccgcgtc ggccgaggtg ctgcgcctgc tgcgccaggc ggtcgacgag ttcggccaga
     6421 ccgtggtcat ggtcacccac gaggcccacg cggccgccta cgccgaccgg ctggtggtgc
     6481 tgcgcgacgg caaggtggcc catgacggcg aggccggcac cgaggcgcag gtcatggagc
     6541 acatgaagga cgcgggctag acccgatgcg aaagctgatc ctcagagggc tggccgagcg
     6601 ccggtcacga ctggccggcg tggtgctggc cgtgttcctg ggcgtgtcgc tggtggcggg
     6661 ctcgctggtg ttcaccgaca ccatcaacgc ctccttcgac aagctgttcg agcgctcgct
     6721 gaagggcagc gacgtcgtgg tcaccccgcg cgaggtcgtg cgccaggacc aggacgagcc
     6781 gccgccgttc tcggcctcgc tgctcagccg ggtgcgcgac accgacgggg tcgaggccgc
     6841 gtcgggcgcc atcttcgccg tcgtccgcct ggtcgacgac aagaacgaga acctgggcgc
     6901 cggcttcgcg cccaacttcg tctcgtccac ggtgcccgag cgtttcgatc cgctgaccta
     6961 caccgacggc cgcgcgccgc ggacggggtc cgaggccgcc atcgacagcg gcacggccga
     7021 gcgcagcggc
//
LOCUS       DASUPW010000156         6352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_36330, whole genome shotgun sequence.
ACCESSION   DASUPW010000156 DASUPW010000000
VERSION     DASUPW010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6352
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36330"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1941
                     /locus_tag="VFY44_03575"
     CDS             <1..1941
                     /locus_tag="VFY44_03575"
                     /inference="COORDINATES: protein motif:HMM:NF005539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5909545.1"
                     /translation="DGTIYVLVREGSRGKVDALVQSLGAEEGRIVPVAGDLFKEKLGV
                     EDFDEKIDHLFHLAAVYDMEADEEASEKANVQGTRNVIEFANAHDVGRFHHVSSIAVA
                     GSYKGVFQEDMFDEDQKLPHHYHRTKYESEAMVRRDVTAQTLVFRPGMVVGHSETGVM
                     DKVDGPYYMFNLLKKLRGALPAWFPLAGPEGGKTTIVPVDFVAKAMDHIAHLPDADLP
                     GDTYHLVDPDPMTTGGALNTFARAAHAPQMAMRVDENLTQAVPAAVRSGVMALPTVKK
                     IRDQLYGDLGIPPAAMEVRDFKCTFDARDTQRALAGTGIAVPPLDSYAGKLWDYWERN
                     MDPALFRDRSLATAIKDKRVMITGASSGIGLETALRIGEAGGTVFLVSRTREKLEEVA
                     RQVEEKGGTAHVFPADLSDLEDLERMAKEVIDEHGGVDVLINNAGRSIRRSVARSYDR
                     FHDYERTMQLNYFGALKLIMTLLPGMRERKSGHIINISSIGVQTNTPRFSAYVASKSA
                     LDAFSRCAAPECVADNVKFTTVYMPLVRTPMIAPTDMYKAFPTMSPEEAAQMLCDAMI
                     DKPKRTASRLGTFGEVLYALSPQTMDIVLNQAYNLFPDSSAAKGKDKNKEVEGGGDGG
                     SGDNEMSTEAVAMAYLLKGVHF"
     gene            complement(1938..2885)
                     /locus_tag="VFY44_03580"
     CDS             complement(1938..2885)
                     /locus_tag="VFY44_03580"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5909546.1"
                     /translation="MPPERQGTTAEAVAAATTFLKALREGLDQGLELPEAVVRSTGVL
                     PGGAKKALSRLDGRYTEDEWGFDEDFARAVEPLFDFLYDQWWRVHATGVSKVPAHGRV
                     LLAGNHAGILPWDATMMSIALLREHPLPRYPRFLVLDWAFQLPWVSTAIRKVGGVVAS
                     PYNALRLLEDDQLVAVFPEGVKGTGKPFSERYRLQRFGRGGFVELALRTGAPIVPVAV
                     VGSEEIYPMLGQSGLAARVLRAPYFPLTPTFPWLGPLGMVPLPSKWRIEFCDPIPTEA
                     YGPDAVNDRALVLELSERVRSTIQQKVYENLVERGGAFV"
     gene            complement(2875..3822)
                     /locus_tag="VFY44_03585"
     CDS             complement(2875..3822)
                     /locus_tag="VFY44_03585"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5909547.1"
                     /translation="MAGRRVLITGVGSYIGSALARRLSADPEVDYVAGLDMRPPAEPV
                     EGMHFIEADLRDQVIATLIPQEDVDVVVHNQIVRRPGPGMSERSMHDINVIGTLQMLA
                     ACERSDSIRAIVIRGSAGIYGSEPQAPQFFSEELARLFPLRTRFQRDVGEIENLFETY
                     ARRHPDVICTMLRYQPAVGPALDTQVTRYLRPPVAPTYLGFDPRIQVVHEEDGLGALL
                     AAVRRPVRGAVNVASPGTIGLTRMIRMAGRAPLPLAAPLFGSATNALTRAGLPSLSPD
                     FRRLLRYGRAVDTTRLVEEVGFTPTYTTPSAMEEFADAA"
     gene            complement(3824..4186)
                     /locus_tag="VFY44_03590"
     CDS             complement(3824..4186)
                     /locus_tag="VFY44_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909548.1"
                     /translation="MPDRPEIPDALREAVERTVQATVETRGKAQGAVDDLTGSVDDLV
                     KGAEKGLSARRRSVRAAVEERLPATQEDIKDLRAELRSIGRRLEAIEERLDKAVAPAK
                     KPAARKPAAKKRSSAKNS"
     gene            4215..4898
                     /locus_tag="VFY44_03595"
     CDS             4215..4898
                     /locus_tag="VFY44_03595"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909549.1"
                     /translation="MPEAIHLRPTAPLAERVLLPGDPHRALAVAQAILEEPRMFNHTR
                     GLWGYTGRARDGELLTVQATGMGAPSAAIVVRELIDLGARTLVRIGTCGALDDGLGLG
                     TLVSAEHALAADGTSRALGAGERVRADPELAAALSGAGAVPVTAVTTDLFYDGGDRLH
                     EWVAAGARVVEMEASAVMAVAAAGGARAAVLLGVTDLLADGGRDRIAGEEYEQLGVRL
                     GEAAWEALS"
     gene            complement(4895..5353)
                     /locus_tag="VFY44_03600"
     CDS             complement(4895..5353)
                     /locus_tag="VFY44_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909550.1"
                     /translation="MTDDHEDQGFVDRLSRQGEEALGRLAEELVANPVVSGAIGRALE
                     AREKAVLAQEAAMGALNLPSAADIERLTRRLRSVSQRLEGIEDALDRLEDKATAAAAA
                     AREAGDGGSEDLSKRLDEIATDLSKLREAVAPVADPVPRAQERLTVTDAS"
     gene            5592..>6352
                     /locus_tag="VFY44_03605"
     CDS             5592..>6352
                     /locus_tag="VFY44_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX5909551.1"
                     /translation="MKNRLAYFVACVALAAAAVATAFTYSAPDPAEAQGGELCVVIGG
                     QKVKVADLTQAQQLLGLPQVPCLDVGTPTTPAPAAPAPTNTTPAPAPTAPAVPKPGAG
                     KEQERGGSKKPASGDEKSGDKKKKSKGKSKSKAKAEIEGGAEDRKGRKGKRKKASRGI
                     RNPDGSPTPSNPGFVDALPGPSTASGVPNFVIRKFRVPVFLLPIYQAAGIQYGIRWEI
                     LAAINEIETDYGRNLNVSSAGALGWMQFMPATWRQ"
BASE COUNT          937 a         2270 c         2294 g          851 t
ORIGIN      
        1 gacgggacca tctacgtgct ggtgcgcgag ggctcgcgcg ggaaggtcga cgcgctggtc
       61 cagagcctgg gcgccgaaga gggacggatc gtccccgtgg ccggtgacct gttcaaggag
      121 aagctggggg tcgaggactt cgacgagaag atcgaccacc tgttccatct ggccgcggtg
      181 tacgacatgg aggccgacga ggaggcctcg gagaaggcca acgtgcaggg gacccgcaac
      241 gtcatcgagt tcgccaacgc gcacgacgtc ggccgcttcc accacgtcag ctcgatcgcg
      301 gtcgccggct cctacaaggg cgtgttccag gaggacatgt tcgacgagga ccagaagctg
      361 ccccaccact accaccggac caagtacgag tccgaggcca tggtccgccg cgacgtgacc
      421 gcgcagaccc tggtcttccg ccccggcatg gtcgtcgggc actccgagac gggcgtcatg
      481 gacaaggtcg acggcccgta ctacatgttc aacctgctca agaagctgcg cggcgcgctg
      541 cccgcgtggt tcccgctggc cgggcccgag ggcggcaaga ccacgatcgt cccggtggac
      601 ttcgtggcga aggcgatgga ccacatcgcc cacctgcccg acgccgacct gccgggcgac
      661 acctaccacc tggtcgaccc cgacccgatg accaccggcg gcgcgctgaa cacgttcgcc
      721 cgggccgcgc acgcgccgca gatggccatg cgcgtggacg agaacctgac ccaggccgtg
      781 cccgccgcgg tgcgctccgg ggtcatggcg ctgcccacgg tcaagaagat ccgcgaccag
      841 ctctacggcg acctgggcat ccccccggcg gccatggagg tgcgcgactt caagtgcacg
      901 ttcgacgcgc gcgacaccca gcgcgccctg gccggcaccg gcatcgcggt gcctccgctg
      961 gactcctacg cgggcaagct gtgggactac tgggagcgca acatggaccc ggcgctgttc
     1021 cgggaccgct cgctggccac ggccatcaag gacaagcgcg tgatgatcac gggcgcctcg
     1081 tcgggcatcg gccttgagac ggccctgcgc atcggcgagg cgggcggcac cgtgttcctg
     1141 gtctcgcgca cgcgggagaa gctggaggag gtggcgcgcc aggtcgagga gaagggcggc
     1201 acggcgcacg tgttcccggc cgacctgtcc gacctcgagg acctcgagcg gatggccaag
     1261 gaggtcatcg acgagcacgg cggcgtggac gtgctgatca acaacgcggg ccgctcgatc
     1321 cgccggtcgg tggcgcggtc ctacgaccgc ttccacgact acgagcgcac gatgcagctg
     1381 aactacttcg gcgcgctcaa gctgatcatg accctcctgc ccggcatgcg cgagcgcaag
     1441 agcggccaca tcatcaacat ctcgtcgatc ggcgtgcaga ccaacacgcc gcgcttctcg
     1501 gcctacgtgg cctcgaagtc ggcgctggac gccttctcac gctgcgcggc gcccgagtgc
     1561 gtggccgaca acgtcaagtt caccaccgtc tacatgccgc tggtgcggac cccgatgatc
     1621 gcgccgaccg acatgtacaa ggcgttcccc accatgtcgc cggaggaggc cgcgcagatg
     1681 ctgtgcgacg cgatgatcga caagcccaag cgcacggcgt cgcgcctggg caccttcggc
     1741 gaggtgctct acgccctgtc gccgcagacg atggacatcg tgctcaacca ggcctacaac
     1801 ctgttccccg attcctcggc ggcgaagggc aaggacaaga acaaggaggt cgaggggggc
     1861 ggcgacggcg gctcggggga caacgagatg tccacggagg ccgtggcgat ggcgtacctc
     1921 ctcaagggag tgcacttcta gacgaacgcc ccgccgcgct ccaccaggtt ctcgtacacc
     1981 ttctgctgga tcgtcgagcg gacccgctcc gacagctcca gcaccagggc gcggtcgttg
     2041 accgcgtccg ggccgtaggc ctcggtcggg atcgggtcgc agaactcgat ccgccacttg
     2101 gagggcagcg ggaccatgcc cagcggcccc agccacggga aggtgggcgt cagcgggaag
     2161 tagggcgcgc gcaggacccg ggcggccagg ccggactggc ccagcatcgg gtagatctcc
     2221 tcgctgccca ccacggccac cggcacgatc ggggcgccgg tgcgcagggc cagctcgacg
     2281 aacccgccgc ggccgaagcg ctggagccgg tagcgctcgg agaagggctt gccggtgccc
     2341 ttgaccccct cggggaacac cgcgacgagc tggtcgtcct ccagcagccg gagcgcgttg
     2401 tagggcgagg ccaccacgcc gccgaccttg cggatggcgg tcgagaccca gggcagctgg
     2461 aaggcccagt ccaggaccag gaagcgcggg tagcgcggca gcgggtgctc gcgcagcagc
     2521 gcgatcgaca tcatcgtcgc gtcccagggc aggatgcccg cgtggttgcc ggccagcagc
     2581 acgcgcccgt gggcgggcac cttcgacacc ccggtggcgt ggacgcgcca ccactggtcg
     2641 tagaggaagt cgaagagcgg ctcgacggcg cgggcgaagt cctcgtcgaa gccccactcg
     2701 tcctcggtgt agcggccgtc caggcggctc agcgccttct tcgcgccacc ggggagcacc
     2761 ccggtggagc gcacgaccgc ctcgggcagc tcgaggccct ggtccaggcc ctcgcgcagc
     2821 gccttcagga acgtggtcgc ggcggcgacc gcctccgccg tggtgccctg gcgctcaggc
     2881 ggcatcggcg aactcctcca tcgcggacgg ggtggtgtag gtgggcgtga agccaacctc
     2941 ctcgaccagc cgggtggtgt ccaccgcccg gccgtagcgc agcaggcgcc ggaagtcggg
     3001 ggacagcgag ggcagtcccg cgcgcgtgag cgcgttcgtg gcgctgccga acagcggcgc
     3061 ggccagcggc agcggcgcgc ggccggccat gcggatcatg cgcgtcaggc cgatcgtccc
     3121 cgggctggcc acgttcaccg ccccgcgcac cggcctgcgg accgcggcca gcagcgcgcc
     3181 caggccgtcc tcctcgtgca cgacctggat ccgcgggtcg aacccgaggt aggtgggcgc
     3241 caccggcggg cggaggtagc gcgtgacctg cgtgtccagg gcgggcccga ctgccggctg
     3301 gtagcgcagc atggtgcaga tcacgtcggg gtggcggcgg gcataggtct cgaacaggtt
     3361 ctcgatctcg ccgacgtcgc gctggaagcg ggtgcgcagc gggaacaggc gggccagctc
     3421 ctcgctgaag aactgcggcg cctggggctc ggagccgtag atgcccgcgg agccgcggat
     3481 gacgatcgcc cggatggagt ccgagcgctc gcacgccgcc agcatctgca gcgtcccgat
     3541 cacgttgatg tcgtgcatgc tgcgctcgga catgccgggc ccggggcggc ggacgatctg
     3601 gttgtgcacc accacgtcga cgtcctcctg cgggatcagc gtcgcgatca cctggtcgcg
     3661 caggtcggcc tcgatgaagt gcatgccctc gaccggctcg gccggagggc gcatgtccag
     3721 cccggccacg tagtccacct cgggatcggc gctcagccgc cgggccagcg cggagccgat
     3781 gtagctcccg acgccggtga tcaggacgcg acggccggcc acgtcaggag ttcttggcgg
     3841 agctgcgctt cttcgccgcg ggcttccgtg ccgcgggctt cttcgccgga gcgacggcct
     3901 tgtccaggcg ctcctcgatc gcctcgagcc ggcggccgat gctccgcagc tccgcgcgca
     3961 ggtccttgat gtcctcctgg gtcgccggca ggcgctcctc gaccgcggcg cggacgctcc
     4021 gtctccgcgc gctcaggccc ttctctgcgc ccttgaccag gtcgtcgacc gagcccgtca
     4081 ggtcgtcgac cgcgccctgc gccttgccgc gggtctccac ggtggcctgc acggtgcgct
     4141 cgacagcctc gcgcagcgcg tcgggtatct cgggccggtc gggcatcgcg ggccccagtc
     4201 tatggtgcgc gcccgtgccc gaggcgatcc acctcagacc caccgcgccc ttggccgagc
     4261 gggtgctgct gccgggcgac ccccaccgcg ccctggccgt ggcccaggcg atcctcgagg
     4321 agccgcggat gttcaaccac acgcgcgggc tgtggggcta cacgggccgg gcccgcgacg
     4381 gcgagctgct gacggtgcag gccaccggca tgggagcgcc gagcgcggcg atcgtggtcc
     4441 gtgagctgat cgacctgggg gcgcgcacgc tggtgcggat cgggacgtgc ggcgccctgg
     4501 acgacgggct cgggctgggc acgctggtca gcgccgagca cgccctggcc gcggacggca
     4561 ccagccgggc cctgggcgcc ggcgagcgcg ttcgcgccga cccggagctg gcggcggccc
     4621 tgagcggcgc cggcgccgtg cccgtcaccg ccgtgaccac cgacctgttc tacgacggcg
     4681 gcgaccggct ccatgagtgg gtggcggccg gcgcgcgggt ggtcgagatg gaggcctcgg
     4741 ccgtgatggc cgtggcggcg gcgggcggcg cccgcgcggc cgtcctgctc ggcgtcaccg
     4801 acctgctggc cgacgggggc cgcgaccgga tcgccggcga ggagtacgag cagctggggg
     4861 tccgcctcgg cgaggcggcg tgggaggcgc tgagctagct cgcgtcggtg acggtgaggc
     4921 gctcctgcgc gcgcggcacc ggatcggcca cgggcgcgac cgcctcgcgc agcttggaga
     4981 ggtcggtggc gatctcgtcc aggcgcttgc tcaggtcctc ggagccgccg tcgccggcct
     5041 cgcgggccgc ggccgcggcg gccgtcgcct tgtcctcgag ccggtccagc gcgtcctcga
     5101 tgccctcgag ccgctgggac acggagcgca gacggcgggt cagccgctcg atgtcggcgg
     5161 cggacgggag gttcagggcg cccatcgcgg cctcctgggc caggaccgcc ttctcgcggg
     5221 cctcgagggc ccgcccgatg gcgccgctca cgacgggatt ggccacgagc tcctcggcca
     5281 ggcgcccgag cgcctcctcc ccctgccgtg agagccggtc gacgaagccc tggtcttcat
     5341 ggtcgtcggt caccgcgggg aggctatcgg aatcggcccc ccgcggaccg gggcgagctc
     5401 ccgccgacag acctcaactc tcaagttgag ccttagactg cgccaccccc gcgatccggc
     5461 tctgcaatga gtgttgtgct ccgatgaagc ggtgacagga aacggcgcta agcgggtaaa
     5521 gcgtctgata gcttgcccgc gtcttacgtg ctccgcctgg ggctcggtgc tgggaggaac
     5581 tgatcgaggg gatgaagaac cggctggcct acttcgtcgc gtgcgtggca ctcgctgccg
     5641 cggcggtcgc caccgcgttc acgtactccg cgcccgaccc ggccgaggcg cagggcggcg
     5701 agctgtgtgt ggtcatcggc ggccagaagg tcaaggtcgc cgacctgacg caggcacagc
     5761 agctcctggg cctgccccag gtcccctgcc tggacgtcgg caccccgacg acacccgcgc
     5821 ccgccgcccc cgcgcccacg aacacgacgc ccgccccggc ccccaccgcc ccggccgtgc
     5881 ccaagcccgg cgccggcaag gagcaggagc gcggcggctc gaagaagccc gcctccggcg
     5941 acgagaagtc cggcgacaag aagaagaagt ccaagggcaa gtcgaagtcg aaggctaagg
     6001 ctgagatcga gggtggagcc gaggatcgca agggccggaa gggcaagcgc aagaaggcgt
     6061 ccaggggcat ccgcaatccc gacggctccc ccaccccgtc gaaccccggc ttcgtcgacg
     6121 cgctcccggg cccctcgacc gcctcgggcg tccccaactt cgtgatccgc aagttccgcg
     6181 tgccggtgtt cctgctgccg atctaccagg ccgccggcat ccagtacggc atccgctggg
     6241 agatcctggc ggccatcaac gagatcgaga ccgactatgg ccgcaacctg aacgtgtcct
     6301 cggccggggc gctgggctgg atgcagttca tgcccgcgac ctggcgccag ta
//
LOCUS       DASUPW010000157         6029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1421746, whole genome shotgun sequence.
ACCESSION   DASUPW010000157 DASUPW010000000
VERSION     DASUPW010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421746"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..489)
                     /locus_tag="VFY44_03610"
     CDS             complement(67..489)
                     /locus_tag="VFY44_03610"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5909552.1"
                     /translation="MTFDPDRPLAGARIATRLPCEDLERARRFYRDVLGLEPAEERDG
                     GLLYHGASGSFALFASSGRSSGESTQMAFEVDDLDAVVAALKERGLEFEEVEIPGFEI
                     RDGIMEVPGNYPSKGGRGERAVWFRDSEGNLMGLGQPL"
     gene            complement(486..1151)
                     /locus_tag="VFY44_03615"
     CDS             complement(486..1151)
                     /locus_tag="VFY44_03615"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX5909553.1"
                     /translation="MARVTGIGGFFFRAKDPDALNRWYAEHFGVIELESKDYDDAGWF
                     QDRGETVFFACEPGSTTPGPPEKEWSINFRVDDLDAMVERLRAAGIEVEPHAQEYPNG
                     RFAELADPEGNHVQLWEPNEASVARDPGPVIVAGHLDLDPADRDAFVADRAEFVRAAR
                     TAPGCLDFAMSADAVEPGRVNVLERWESKAALHAFRGDGPDDDSSARVVGFDVHDYPV
                     ASP"
     gene            complement(1275..1775)
                     /locus_tag="VFY44_03620"
     CDS             complement(1275..1775)
                     /locus_tag="VFY44_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX5909554.1"
                     /translation="MSLNPALDGVHLCPRCGAEADVSPPRSLICPDCGYKAYYNPTPA
                     AGAIPFGSDGRIWLMRRGTDPGRGLWTFPGGFVDLGESVPDAARREAREEMAVDLEVG
                     SLVGVYSRPDDRVLLVVYRASLPDGQVPAVSEEAAEVRAFAAGELPWDELAFWSTELA
                     LRDVLG"
     gene            complement(1772..2755)
                     /locus_tag="VFY44_03625"
     CDS             complement(1772..2755)
                     /locus_tag="VFY44_03625"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5909555.1"
                     /translation="MQQRKLGTEGLEVGAIGLGTMGMTMAYGAGDEEGGAATIERAHE
                     LGVTLFDTAELYGGGTGSNEQLVGRAVAGFRDEIVIATKFGFDMSADPIGSAFDSRPE
                     HIREVTENSLRYLGTDHIDLLYQHRVDPDVPIEDVAGTVKELIDEGKVRFFGLSEAGP
                     DVIRRAHEVQPVSALQTEYSIFERAVEADVLPVVRELGIGFVPYSPLGRGFLTGDVKP
                     ASEYPTDDMRSWDDRWQPGNYEKNVRAIEELTTLASEKGIAVTQLALAWLLAQGDDVV
                     PIPGTRNPERLAQNVSAADLELTQDDLARIAEIVPQGAFGSRYPQAMMPSW"
     gene            3020..3769
                     /locus_tag="VFY44_03630"
     CDS             3020..3769
                     /locus_tag="VFY44_03630"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX5909556.1"
                     /translation="MRIAVAGGTGQVGRHTVSALQEAGHEAVPLSRSAGVDVVTGEGL
                     DEALAGSQAVVDATNTTAADPDETRAFFGSATANLLDAGRRAGVRHHVVLSIVRVDEI
                     EGNAHYAGKRRQEELVREGPLPFTIVRAPQFFEFPEMVLGWTRDGDVAVVPPMKARAA
                     SAEDVGRALADIALSEPRDDVLEVVGPEVVDLADLARRATAARGDTVTVDAAWEGTPF
                     TEEMAGPPEPAPGALVLPTTAEEWLAGIGRG"
     gene            complement(3766..4449)
                     /locus_tag="VFY44_03635"
     CDS             complement(3766..4449)
                     /locus_tag="VFY44_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5909557.1"
                     /translation="MSLPPGQRALDEFPRFGTHMAYPPPRVLVDHAIAVGGAVADPFS
                     VPLAALADLPRREQTADFHCVAGWSATGLRWEGVPFARFYAEVIEPALSPGASITHVT
                     FQGRDGYRSTACLEDVLAEDVLIAEHLDGRPLDGDHGAPVRLVSPSQYGFISTKHLCR
                     IEVHTAEPQARYHRNRLNHLGLRLVEPHPRARVWEEERNGYLPAWAVRLPYRMLIPPI
                     RALAARGRR"
     gene            complement(4446..5042)
                     /locus_tag="VFY44_03640"
     CDS             complement(4446..5042)
                     /locus_tag="VFY44_03640"
                     /inference="COORDINATES: protein motif:HMM:NF022517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HEX5909558.1"
                     /translation="METAMALPESAHTSQPWRIHEIAHDFRLEDVWALPTPGGPHDFP
                     RLVEAFGSFDPGRDSSPPVRFLFALRWKIGELLKLDEPRTGTGSRVPSLRDRVPADLR
                     DGPAGPRSDTLPFTPLYLTENEWAGEIANQTMHGVLHVGWVPDGDGGYHGQMAVLVKP
                     NGLMGTAYMAAIAPFRHLVVYPQMLRDIGRRWRAAAAA"
     gene            5093..5656
                     /locus_tag="VFY44_03645"
     CDS             5093..5656
                     /locus_tag="VFY44_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5909559.1"
                     /translation="MAAPTRTPRESWIQEGLKALAAGGPDAVRVEALAAALGVTKGGF
                     YWHFDGRQALLDEMLDLWEREMVDEVIARVEKRGGDGRAKLVHLFSLAGAIGERLAIE
                     LAVRDWARRDKAVARRLRRVDNRRMDYMRPLFAEFCADEDDAEARTMLAFSLFVGSHF
                     LAASHAGRSRAQVMRLATERLLSERWS"
     gene            complement(5637..>6029)
                     /locus_tag="VFY44_03650"
     CDS             complement(5637..>6029)
                     /locus_tag="VFY44_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909560.1"
                     /translation="IGSAVNDATRELGGTLGVAVIGSVALSLYRGAFDGLRLPAAATD
                     AARESLGGAFQAAGALPPPLADSLRQRAEDGFLDGMAAGCLVAGGVALAGAVMVAWFL
                     PSQPAAAPQEEGQTGLAPEPAAVSSSAR"
BASE COUNT          819 a         2245 c         2182 g          783 t
ORIGIN      
        1 gagagctggt cggtggccac gccccgagcg tactcaaccg attggttgaa tacaagcctt
       61 cggtcgctag agcggctgcc ccaggcccat caggttgccc tcgctgtcac ggaaccacac
      121 ggcccgctcg ccgcggccac ccttgctcgg gtagttgccc ggcacctcca tgatcccgtc
      181 gcggatctcg aagccgggga tctcgacctc ctcgaactcc agcccgcgct ccttcagcgc
      241 cgccaccacc gcgtccaggt cgtcgacctc gaacgccatc tgggtggact cgccgctgga
      301 gcggcccgag gaggcgaaca gggcgaacga gcccgacgcg ccgtggtaga gcaggccgcc
      361 gtcgcgctcc tccgccggct cgaggcccag cacgtcgcgg tagaagcgcc gcgcgcgctc
      421 caggtcctcg cagggcaggc gggtggcgat gcgggcgccc gccagggggc ggtcggggtc
      481 gaaggtcacg ggctcgccac cgggtagtcg tgcacgtcga agccgacgac ccgcgccgag
      541 ctgtcgtcgt ccggcccgtc gccgcggaac gcgtggagcg ccgccttcga ctcccaccgc
      601 tcgagcacgt tcacgcgtcc cggctcgacg gcgtcggccg acatggcgaa atccaggcag
      661 cccggcgccg tgcgcgcggc gcgcacgaac tcggcgcggt cggccacgaa ggcgtcgcgg
      721 tcggcagggt cgagatccag atggccggcc acgatcaccg ggcccgggtc gcgtgccacc
      781 gacgcctcat tgggctccca cagctggacg tggttgccct cggggtcggc cagctcggcg
      841 aaacgcccgt tggggtactc ctgggcgtgg ggctcgacct cgatgccggc cgcgcgaagc
      901 cgctcgacca tcgcgtccag gtcgtcgacg cggaagttga tggaccactc cttctccggc
      961 gggccgggcg tggtcgagcc gggttcgcag gcgaagaaca cggtctcgcc gcggtcctgg
     1021 aaccagcccg cgtcgtcgta gtccttggac tccagctcga tcacgccgaa gtgctcggcg
     1081 taccagcggt tcagcgcgtc cgggtccttc gcgcggaaga agaagccacc gatgccggtc
     1141 acgcgggcca tggagtggaa cctatccggg cccgcgagca cggtgggtcg tcgcgggttc
     1201 gtaggcgtgc gccggcgcac tcctcctgac ccggaacgcc gcgaccgcgc cgcgggtcgg
     1261 tacgtgcgcc ccgcctagcc cagcacgtcg cgcagcgcca gctcggtcga ccagaacgcc
     1321 agctcgtccc acggcagctc gccggccgcg aacgcgcgca cctcggccgc ctcctcgctg
     1381 accgcgggga cctggccgtc gggaagcgaa gcccggtaga ccaccagcag cacgcgatcg
     1441 tcgggacgcg agtacacgcc cacgagcgag ccgacctcca ggtcgaccgc catctcctcg
     1501 cgcgcctcgc gccgcgcggc gtcgggcacc gactcgccga ggtccacgaa gccgccgggg
     1561 aacgtccaca gcccgcggcc cgggtcggtg ccgcgacgca tcagccagat ccggccgtcg
     1621 gagccgaacg ggatcgcgcc cgccgcgggc gtggggttgt agtacgcctt gtagccgcag
     1681 tcggggcaga tcagcgagcg cggcggcgac acgtccgcct cggccccgca gcgcgggcag
     1741 aggtggacgc cgtcgagggc ggggttcagg ctcaccagct gggcatcatc gcctgggggt
     1801 accggctgcc gaacgcgccc tggggcacga tctcggcgat ccgggccagg tcgtcctgcg
     1861 tcagctccag gtcggcggcg ctcacgttct gcgccaggcg ctccgggttc cgggtcccgg
     1921 ggatgggcac gacatcgtcg ccctgggcca gcagccaggc cagcgccagc tgggtgaccg
     1981 cgatgccctt ctcggaagcc agcgtggtca gctcctcgat ggcgcgcacg ttcttctcgt
     2041 agttgcccgg ctgccagcgg tcgtcccagc tgcgcatgtc gtcggtgggg tactccgagg
     2101 ccggcttcac gtcgccggtc aggaacccac gacccagcgg cgagtacggc acgaacccga
     2161 tccccagctc gcgcaccacg ggcaggacgt cggcctccac cgcgcgctcg aagatcgagt
     2221 actcggtctg cagcgcggac accggctgca cctcgtgcgc gcgccggatc acgtccggcc
     2281 cggcctcgct gaggccgaaa aagcgcacct tgccctcgtc gatcagctcc ttgaccgtgc
     2341 cggccacgtc ctcgatcggc acgtcggggt cgacccggtg ctggtagagc aggtcgatgt
     2401 ggtcggtgcc caggtagcgc aggctgttct cggtgacctc gcggatgtgc tcgggccggg
     2461 agtcgaacgc gctgccgatg gggtcggcgc tcatgtcgaa gccgaacttg gtggcgatca
     2521 cgatctcgtc gcggaagccc gcaacggccc tgcccaccag ctgctcgttg gacccggtgc
     2581 cgccgccgta gagctcggcg gtgtcgaaca gggtcacccc gagctcgtgg gcgcgctcga
     2641 tcgtcgcggc gccgccctct tcgtcgccgg ccccgtaggc catggtcatg cccatcgtcc
     2701 ccaggccgat ggcgccgacc tcgagtccct cggttccaag cttgcgctgc tgcatggtgc
     2761 tcctcacgtc aggtttgcga tgccctgacg ctagtcctcg ccacgcgggg cttctgcggg
     2821 gggcggccgc cgcgagcacc ccatgagcga gaccaaaccc gagctcgcgg cggaccgcca
     2881 acgcatcggg ccaaggtagg cccactcgcg cccgtgcgcg aaaagttcag aggcaccccc
     2941 cgaggtgtac gggtggccgt acacccgggc cgggcgacgg cggctagcgt cgacggcacc
     3001 cggagcgaag gagatcccaa tgcgcatcgc agtcgccggc ggcacgggcc aggtgggccg
     3061 ccacaccgtg tcggcactcc aggaggcggg ccacgaggcg gtgccgctct cacgctcggc
     3121 gggggtcgac gtggtcaccg gggaggggct cgatgaagcg ctggccggca gccaggcggt
     3181 ggtcgacgcg accaacacca ccgccgccga tccggacgag acgcgcgcct tcttcggctc
     3241 ggccaccgcc aacctgctgg acgccggccg gcgcgccggc gtgcgccacc acgtggtcct
     3301 ctcgatcgtc cgcgtggacg agatcgaggg caacgcccac tacgccggca agcgccgcca
     3361 ggaggagctg gtccgcgagg gcccgctgcc gttcacgatc gtgcgcgctc cccagttctt
     3421 cgagttcccc gagatggtcc tgggctggac ccgcgacggc gacgtcgcgg tggtgccacc
     3481 gatgaaggcg cgcgccgcct cggccgagga cgtgggacgc gccctggccg acatcgccct
     3541 gtccgagccg cgcgacgacg tgctcgaggt ggtcggcccc gaggtggtcg acctggccga
     3601 cctggcccgg cgggcgaccg ccgcccgcgg cgacaccgtg accgtcgacg ccgcctggga
     3661 ggggaccccg ttcaccgagg agatggccgg cccgcccgag ccggctcccg gtgcgctcgt
     3721 ccttccgacc acggccgagg agtggctcgc cgggatcggc cggggctagc ggcgcccgcg
     3781 ggcggccagc gccctgatcg gcgggatcag catccggtag ggcagccgca ccgcccacgc
     3841 cggcaggtac ccgttgcgtt cctcctccca cacccgcgcg cgggggtggg gctcgaccag
     3901 gcgcaggccc aggtggttga gccggttgcg gtggtaccgc gcctgcggct cggccgtgtg
     3961 gacctcgatc cggcacaggt gcttggtgga gatgaagccg tactggctcg ggctgaccag
     4021 ccgcacgggc gcgccgtggt cgccgtccag cggccgcccg tccaggtgct cggcgatcag
     4081 cacgtcctcg gccagcacgt cctccaggca cgcggtcgac cggtagccgt cgcggccctg
     4141 gaaggtcacg tgggtgatgg atgcgcccgg gctcagcgcc ggctcgatca cctcggcgta
     4201 gaagcgcgcg aacggcaccc cctcccagcg cagcccggtc gccgaccacc cggcgacgca
     4261 gtggaagtcg gcggtctgct cgcggcgcgg caggtcggcc agcgcggcca gcgggaccga
     4321 gaacgggtcg gccaccgcgc cgccgacggc gatcgcgtgg tcgaccagca cgcgcggggg
     4381 cgggtaggcc atgtgggtgc cgaagcgcgg gaactcgtcc agcgcgcgtt ggccgggggg
     4441 caggctcacg ccgcggccgc ggcgcgccac cgccgcccga tgtcgcgcag catctgcggg
     4501 tagaccacca ggtgccggaa cggggcgatg gccgccatgt aggccgtgcc catcaggccg
     4561 ttgggcttga ccagcacggc catctgcccg tggtagccac cgtcgccgtc gggcacccag
     4621 cccacgtgca gcaccccgtg catggtctgg ttggcgatct cccccgccca ctcgttctcg
     4681 gtcaggtaca ggggcgtgaa cgggagcgtg tccgagcggg ggcccgccgg gccgtcgcgc
     4741 aggtcggccg gcacgcggtc gcgcagcgag ggcacccggg agccggtccc cgtccgcggc
     4801 tcgtccagct tgagcagctc tccgatcttc caccgcaggg cgaacaggaa gcgcaccggc
     4861 ggcgaggagt cccggccggg gtcgaacgag ccgaacgcct ccaccagccg cgggaagtcg
     4921 tgaggcccgc ccggcgtggg cagcgcccag acgtcctcca gccggaagtc gtgcgcgatc
     4981 tcgtggatgc gccagggctg cgaggtgtgg gcggactcgg ggagggccat cgcggtctcc
     5041 atccatacgg ttacgtatcc atacggtacg gtatagatgc ccgcgagtca acatggccgc
     5101 ccccacccgc accccacgag agagctggat ccaggagggc ctgaaggccc tggccgccgg
     5161 cggccccgac gcggtccggg tcgaggcgct ggccgccgcc ctgggcgtca ccaagggcgg
     5221 cttctactgg cacttcgacg gccgccaggc gctgctggac gagatgctcg acctgtggga
     5281 gcgcgagatg gtcgacgagg tcatcgcgcg cgtcgagaag cgtggcggcg acggccgggc
     5341 gaagctcgtg cacctgttct cgctggccgg cgccatcggc gagcggctgg cgatcgagct
     5401 ggccgtccgc gactgggccc ggcgcgacaa ggcggtcgcc cgccgcctgc gccgcgtcga
     5461 caaccgccgg atggactaca tgcgcccgct gttcgccgag ttctgcgcgg acgaggacga
     5521 cgccgaggcc cgcaccatgc tcgccttctc gctgttcgtg ggcagccact tcctggcggc
     5581 ctcgcacgcc ggccgctcgc gcgcgcaggt catgcggctg gccaccgagc ggctgctcag
     5641 cgagcgctgg agctgacggc cgccggctcg ggcgccaggc ccgtctgtcc ttcctcctgc
     5701 ggcgccgcgg ccggctgcga gggcaggaac cacgccacca tcaccgcgcc cgccagcgcg
     5761 acgccgcccg ccaccaggca cccggccgcc atcccgtcca ggaatccgtc ctccgcccgc
     5821 tggcgcaggg agtccgccaa gggcgggggc agggcaccgg cggcctggaa ggccccgcca
     5881 agcgactcgc gcgcggcgtc ggtggccgcc gcaggcaggc gcaggccgtc gaacgcgccg
     5941 cggtagaggc tcaaggccac cgatccgatc accgccaccc ccagcgtgcc gcccagctcg
     6001 cgggtcgcgt cgttcaccgc cgagccgat
//
LOCUS       DASUPW010000158         3170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_175369, whole genome shotgun sequence.
ACCESSION   DASUPW010000158 DASUPW010000000
VERSION     DASUPW010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175369"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1637)
                     /locus_tag="VFY44_03655"
     CDS             complement(3..1637)
                     /locus_tag="VFY44_03655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5909561.1"
                     /translation="MASVASRARSLPRPNPSLLTSTPVIVAVVAGLLLISLYMRTRAL
                     GASLWMDEGLSIGIASQPFFDIPGVLKQDGAPPLYYMMLHVWMQVFGNGPSETQGLSV
                     AISLIAVPGGLWAGWTLFNRRAGLICAALAAFNPFLTIYAQETRMYSLMVVLSLLTCA
                     AFLHVYVYGHRKYLPLFAVLVAAILYTHSWGIFVTAGLIVSLIPPLLATDDRRAFFKD
                     VLIGFTGALILFLPWLPTLVYQAQHTGAPWLNSPRFGAPIQISKSLLGGGTVTVALIL
                     AAGSGLAAVLAARTPSRGTGGTGSEYGAPASRDRTAVYTALSIGVATLAVAWLFSQVT
                     PAWTTRYLGVALGPIFLLASFGLSRAGKLGLVALVIILGIWAVPKTKNLENKSNVSDL
                     AAQANPKLKKGDLVVTLQPEQQPLVNYNTKPGLIHATQLGRPATFGVMDWRDAYDKLK
                     DATPAKNLKPLTDRLPVGSHVLLVHPVTSNIGEWDAPWTQLVRRRSAQWGQAMELDKR
                     FRRDEAVPSFYRRARRIGVRGVLYTKISRTAPRDER"
     gene            complement(1651..>3170)
                     /locus_tag="VFY44_03660"
     CDS             complement(1651..>3170)
                     /locus_tag="VFY44_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909562.1"
                     /translation="GRPPVLGIHWVLLICLALSALTLLFPSTPTYDPWAWLLWGREIT
                     HFDLVTDGGPSWKPLPVMFNVPFSLFGADVAPYLWLWIARAGALLALAMSFRLARRLV
                     GRGAAGVVAGVFAAAFLLTTYQYVRDAALGNSEALLAGLFLWAFERHLDGRRDHALYL
                     GLAVALLRPEAWPFLGLYGLWLFVREPAMRLKVALVGLAIPLLWFGPELWGSGEPFRA
                     SSRAGKPNPGSAAFADHPGLEVAKRFAERAVIPQQVAGFVGVIVAALAWVRHRAQGAI
                     LALAGIGIAWILLVAVMTERGYAGNQRYLIVTTAALCVLGGIGIGRIFDGIREAVAER
                     SSARTGLRVAFAVFAVGLVALSPVIKAKVENVDKTLDKLRYEAALWHTLPGVVDKAGG
                     PDKLLACGNVYSGPFQTQMVAYQLGVHGINVGDVRPLKVSPAPGVIFRTRTVPGGPLV
                     ATVPDKRFRRAARSGRWQVWTAPRADARGRDCPAAGTDAPRVPPTARTTELSSSR"
BASE COUNT          518 a         1056 c         1208 g          388 t
ORIGIN      
        1 cgttatcgct catctcgggg ggctgttcgg gagatcttcg tgtaaaggac tccgcgtacc
       61 ccgatccggc gggcacggcg gtagaaggaa ggcactgcct catcgcgccg gaatcgcttg
      121 tccagctcca tcgcctgacc ccattgggcc gaacggcgac ggacgagttg ggtccaggga
      181 gcgtcccatt cgccgatgtt gctcgtgacg ggatgcacga ggaggacgtg actgccgacg
      241 ggcagcctat cagtgagtgg cttgaggttc ttggcgggcg tggcgtcctt gagcttgtcg
      301 taggcgtcgc gccagtccat gaccccgaac gtggccggcc ggccgagctg ggtcgcgtgg
      361 atcaggccgg gcttggtgtt gtagttgacc agcggctgct gctcgggctg gagggtgacc
      421 acgaggtcgc ccttcttgag cttggggttg gcctgggccg ccaggtcgga gacgttggac
      481 ttgttctcca ggttcttcgt cttggggacg gcccagatgc ccaggatgat caccagcgcc
      541 accaggccga gcttgcccgc gcgtgacagg ccgaacgagg ccagcaggaa gatcgggccc
      601 agggcgacgc ccaggtagcg ggtggtccag gcgggcgtga cctgcgagaa cagccacgcg
      661 acggccaggg tggccacgcc gatggacagc gccgtgtaga ccgcggtgcg gtcgcgcgac
      721 gcgggcgcgc cgtactcgct gccggtgccg cctgtgcccc gcgatggggt acgcgcggcc
      781 aggacggcgg ccaggccgga gccggcggcc aggatcagcg cgaccgtgac ggtgccaccg
      841 cccagcagcg acttggatat ctggatgggc gcgccgaagc ggggcgagtt gagccacggc
      901 gccccggtgt gctgggcctg gtagaccagc gtgggcagcc agggcaggaa caggatcagc
      961 gcgccggtga agccgatcag cacgtccttg aagaacgcgc gccggtcgtc ggtggccagc
     1021 agcggcggga tcagcgagac gatcaggccc gcggtgacga agatgcccca cgagtgcgtg
     1081 tagaggatgg ccgcgaccag gaccgcgaac agcggcaggt acttgcggtg gccgtagacg
     1141 tacacgtgca ggaaggcggc gcaggtgagc aggctgagca ccaccatcag cgagtacatc
     1201 cgcgtctcct gcgcgtagat cgtcaggaac gggttgaagg cggccagggc ggcgcagatc
     1261 aggcccgcgc gacggttgaa cagcgtccag ccggcccaca gcccgccggg gacggcgatc
     1321 agggagatgg cgaccgacag cccctgggtc tcggacgggc cgttgccgaa gacctgcatc
     1381 cagacgtgca gcatcatgta gtagagcggc ggcgccccgt cctgcttgag cacgcccggg
     1441 atgtcgaaga agggctgcga ggcgatcccg atcgacaggc cctcgtccat ccacagcgac
     1501 gcgcccagcg cgcgcgtgcg catgtacagc gagatcagca gcagtcccgc gacgacggcc
     1561 acgatcaccg gggtcgacgt gagcagcgag gggttggggc gtgggagcga gcgcgctcgc
     1621 gatgcgaccg aggccatcag ggaaagatag tcagcgcgag gacgacagct cggtcgtgcg
     1681 cgcggtcgga gggacacggg gtgcgtcggt gccggcggcc gggcagtcgc gcccgcgggc
     1741 gtcggcccgc ggggcggtcc atacctgcca ccggcccgag cgggccgcgc ggcggaagcg
     1801 cttgtccggc acggtggcca ccagcgggcc gccgggcacg gtgcgggtgc ggaagatcac
     1861 gccgggcgcc ggggacacct tcagcgggcg gacgtcgccg acgttgatgc cgtgcacgcc
     1921 cagctggtag gccaccatct gggtctggaa cgggccgctg tagacgttgc cgcaggccag
     1981 cagcttgtcc gggccaccgg ccttgtcgac cacgccgggc agcgtgtgcc agagggcggc
     2041 ctcgtagcgc agcttgtcca gggtcttgtc cacgttctcg accttcgcct tgatcaccgg
     2101 cgacagggcc acgaggccca cggcgaacac ggcgaaggcc acccgcagcc ccgtgcgcgc
     2161 gctgctgcgc tcggccaccg cctcgcggat gccgtcgaag atgcgcccga tgccgatgcc
     2221 gccgagcacg cacagggcgg ccgtggtcac gatcaggtag cgctggttgc cggcgtagcc
     2281 gcgctcggtc atcacggcca ccagcaggat ccacgcgatg ccgattcccg ccagcgccag
     2341 gatggcgccc tgggcccggt gccgcaccca ggccagggcg gccacgatca cgccgacgaa
     2401 gcccgcgacc tgctggggga tcaccgcgcg ctcggcgaac cgcttggcca cctcgagccc
     2461 cgggtggtcg gcgaaggcgg cgctgccggg gttgggcttg ccggcgcggc tggaggcccg
     2521 gaagggctcg cccgagcccc acagctccgg gccgaaccac agcagcggga tggccaggcc
     2581 gaccagggcc accttgagcc gcatcgcggg ctcgcgcacg aacagccaca ggccgtacag
     2641 gcccaggaac ggccacgcct ccgggcgcag cagcgccacg gccaggccca ggtagagcgc
     2701 gtggtcgcgg cgcccgtcca agtgccgctc gaaggcccac aggaacaggc cggccagcag
     2761 cgcctcggag ttgccgagcg cggcgtcgcg cacgtactgg taggtggtca gcaggaaggc
     2821 ggcggcgaag acgccggcca ccacacccgc cgcgccgcgg ccgaccagcc ggcgcgccag
     2881 gcggaaggac atggccagcg ccagcagggc gcccgcgcgg gcgatccaca gccacaggta
     2941 gggcgcgacg tcggcgccga agagcgagaa cgggacgttg aacatgaccg gcagcggctt
     3001 ccacgacggg ccgccgtccg tgaccaggtc gaagtgggtg atctcgcgcc cccacagcag
     3061 ccaggcccag gggtcgtagg tcggtgtgga cgggaacagc agggtcagcg ccgacagcgc
     3121 aaggcagatc agcagcaccc agtggatgcc caggacgggc gggcggccgg
//
LOCUS       DASUPW010000159         3263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1976470, whole genome shotgun sequence.
ACCESSION   DASUPW010000159 DASUPW010000000
VERSION     DASUPW010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1976470"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VFY44_03665"
     CDS             complement(<1..330)
                     /locus_tag="VFY44_03665"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX5909563.1"
                     /translation="MSATTETGIERLAAAFAGVGGRAALMPYLMGGHPDIEQSKACCA
                     AAADAGADLIELGVPFSDPLADGPVIHAAGTKALDAGVTPYDVLDVCADASRRLPVVL
                     MVYANVVL"
     gene            complement(327..1499)
                     /gene="trpB"
                     /locus_tag="VFY44_03670"
     CDS             complement(327..1499)
                     /gene="trpB"
                     /locus_tag="VFY44_03670"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX5909564.1"
                     /translation="MSASEAPGREREFGPYGGRFVPETLIPALDELEAAWLEAREDPA
                     YTTELAELLRDYAGRPTPLYRARRLSAELGRTVYLKREDLLHTGAHKINNALGQALLA
                     KRMGKSRVIAETGAGQHGVGIATACALLDLECVVYMGAEDMRRQKPNVERMNLLGASV
                     ESVDIGARTLKEAVSAAIRDWVANVGDTHYVIGSAVGPAPYPAMVRDLQRTIGDEIRE
                     QIQAAEGRLPDRLIACVGGGSNAIGAFAPFVGDRDVTLVGVEAAGDGLESGRHSAPLT
                     TGARGVLHGALAAVMQDDDGQILEAHSVSAGLDYPGAGPEHAWLRDEGRVTYDAVGDA
                     DAVAAFRRLARLEGIIPALEPSHALAWLMANPAGELDVLVLSGRGDKDLAEVLEQP"
     gene            complement(1496..2143)
                     /locus_tag="VFY44_03675"
     CDS             complement(1496..2143)
                     /locus_tag="VFY44_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX5909565.1"
                     /translation="MGTKIKICGITDPEDARLAVELGAWALGLNFWPESVRTCPADAA
                     EDIAASFKRSVELAGVFVNQTLDEIATAADRYELSLIQLHGDEGPAYCREVARRTGAR
                     VIKAARVRDAASVRDLEAFHTDFHLLDAAVPGQRGGTGQSFSWELAGTHRGAVPLILA
                     GGLTPDNVGEAVRVASPYAVDTASGTEASPGHKDPARMTAFFRAVRTTEPEPSTA"
     gene            complement(2377..>3263)
                     /locus_tag="VFY44_03680"
     CDS             complement(2377..>3263)
                     /locus_tag="VFY44_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5909566.1"
                     /translation="HIITNQHVVAGADEFRVRFGEDGEPIEAELLGTDPSVDLALLKV
                     DPADVKDLKVLPLGDSSDARVGAPTIAIGNPFGFTRTVTTGIVSAVQRTIEAPNNFSI
                     ADVIQTDAAINPGNSGGPLLDGQGNVIGINAQIATGGQSSGGSIGIGFAIPVNTLKKL
                     LPDLKAGQSIKRGFLGVTTASVNNEVADQLKLSVKSGAIIQDVTEGGPADKAGLRAGG
                     NPTADGLASGGDVIVAVDGKPVKSSDELVAIIAGKKPGDEVEIEYLRGRSGDRKTATV
                     TLGSRPAKVDTQNQPQLP"
BASE COUNT          396 a         1193 c         1205 g          469 t
ORIGIN      
        1 gagcaccacg ttcgcgtaga ccatcagcac caccggaagg cgccgggaag cgtcggcgca
       61 gacgtccagg acgtcgtagg gggtcacgcc ggcgtccagc gccttggtcc cggcggcgtg
      121 gatcacgggg ccgtcggcca gcgggtcgga gaacggcacg cccagctcga tcaggtcggc
      181 gcccgcgtcc gccgcggcgg cacagcacgc cttcgactgc tcgatgtccg ggtgcccgcc
      241 catcaggtag ggcatcagcg cggcccgccc gcccacgccg gcgaacgcgg cggccaggcg
      301 ctcgatcccg gtctccgtgg tggcgctcac ggctgctcga gcacctcggc caggtccttg
      361 tcgccgcggc cggagaggac cagcacgtcc agctcgcccg cggggttggc catcagccag
      421 gccagcgcgt gcgagggctc cagcgcgggg atgatcccct ccaggcgcgc gagcctgcgg
      481 aaggcggcca cggcgtccgc atcgcccacc gcgtcgtagg tcacccggcc ctcgtcgcgc
      541 agccaggcgt gctcggggcc cgcgcccggg tagtccaggc cggccgagac cgagtgggcc
      601 tccaggatct ggccgtcgtc gtcctgcatg accgccgcca gggcgccgtg cagcaccccg
      661 cgcgcgccgg tggtcagcgg cgcggagtgg cgcccgcttt ccagcccgtc gccggcggcc
      721 tcgacgccca ccagcgtcac gtcgcggtcg cccacgaacg gggcgaacgc gccgatggcg
      781 ttggagccgc cgcccacgca ggcgatcagc cggtcgggca gccgcccctc ggcggcctgg
      841 atctgctcgc ggatctcgtc gccgatcgtg cgctggaggt cgcgcaccat cgccgggtag
      901 ggcgccgggc cgacggccga gccaatcacg tagtgggtgt cgcccacgtt ggccacccag
      961 tcgcggatgg cggcgctgac cgcctccttg agcgtgcgcg cgccgatgtc cacggactcc
     1021 accgaggcgc ccagcaggtt catccgctcc acgttgggct tctggcggcg catgtcctcg
     1081 gcgcccatgt acacgacgca ctccaggtcc agcagcgcgc aggcggtggc aatgcccacg
     1141 ccgtgctggc cggcgcccgt ctcggcgatc acgcggctct tgcccatccg cttggccagc
     1201 agcgcctgcc ccagggcgtt gttgatcttg tgcgcgccgg tgtgcagcag gtcctcgcgc
     1261 ttgaggtaga ccgtccggcc cagctccgcg gacagccgcc gggcgcggta cagcggcgtg
     1321 ggccggcccg cgtagtcgcg cagcagctcg gccagctcgg tggtgtaggc cgggtcctcg
     1381 cgggcctcca gccacgcggc ctcgagctcg tccagcgccg ggatcagcgt ctcgggcacg
     1441 aagcggccgc cgtaggggcc gaactcgcgc tcgcgcccgg gtgcctcgga cgcgctcatg
     1501 ccgtgctggg ctcgggctcg gtggtccgga cggcgcggaa gaacgcggtc atcctggcgg
     1561 ggtccttgtg gcccggcgac gcctccgtgc cgctggccgt gtcgaccgcg tacggagagg
     1621 ccacgcggac ggcctcgccc acgttgtccg gggtcaggcc gccggccagg atcagcggca
     1681 ccgcgccgcg gtgcgtcccg gccagctccc agctgaagct ctggcccgtg cccccgcgct
     1741 ggcccggcac ggccgcgtcc agcaggtgga agtcggtgtg gaaggcttcc aggtcgcgca
     1801 ccgaggcggc gtcgcgcacg cgcgcggcct tgatcacccg ggcaccggtg cgccgggcga
     1861 cctcacggca gtaggcgggc ccctcgtctc cgtgcaactg gatcaggctc agctcgtacc
     1921 ggtcggcggc cgtggcgatc tcgtcgagcg tctggttcac gaacaccccg gccagctcga
     1981 cgctccgctt gaacgatgcg gcgatgtcct cggccgcgtc ggccgggcac gtgcgcacgc
     2041 tctcgggcca gaagttcagc cccagcgccc acgcgcccag ctcgacggcc aggcgcgcgt
     2101 cctcgggatc ggtgatcccg cagatcttga tcttcgtgcc cacgctccaa agggtcgcag
     2161 gtggcgccgc cggccagggc gcccggcggg gcgttcgacc accggccggc cacgcgcggt
     2221 ggatttctcc cgctgaccgg gagaaatgca ccgcgggtcg atccgggcgt cacggatccg
     2281 cgccgaccgt cacggaaggc gcacaggtcc gccgccctac ccagccaagc cgttgcgccg
     2341 gacccgccgg ggccgctgtc ggaaggccag ccggcgctac ggcagctggg gctggttctg
     2401 cgtgtcgacc ttggcgggcc ggctgcccag cgtgacggtg gccgtcttgc ggtcaccgct
     2461 gcggccgcgc aggtactcga tctcgacctc gtcgccgggc ttcttgccgg cgatgatggc
     2521 caccagctcg tccgagctct tcacgggctt gccgtcgacg gccacgatca catcgccgcc
     2581 tgaggcgagg ccgtccgcgg tcgggttgcc gccggcacgc aggcccgcct tgtcggccgg
     2641 gccgccctcg gtcacgtcct ggatgatcgc gcccgacttc acggacagct tgagctggtc
     2701 ggccacctcg ttgttcacgc tggcggtggt cacgcccagg aagccgcgct tgatcgactg
     2761 gccggccttg aggtcgggca ggagcttctt gagcgtgttc acggggatgg cgaagccgat
     2821 gccgatcgag ccgccggagg actggccgcc ggtggcgatc tgggcgttga tgccgatcac
     2881 gttgccctgc ccgtccagga gcgggccgcc cgagttgccg gggttgatgg ccgcgtcggt
     2941 ctggatcacg tcggcgatgc tgaagttgtt gggcgcctcg atggtgcgct ggaccgcgga
     3001 gacgatgccc gtggtgaccg tgcgggtgaa gccgaacggg ttgccgatgg cgatggtcgg
     3061 ggcacccacg cgggcgtccg aggagtcgcc cagcggcagc accttgaggt ccttgacgtc
     3121 ggccgggtcg accttgagca gcgcgaggtc cacggacggg tcggtgccga ggagctcggc
     3181 ctcgatcggc tcgccgtcct cgccgaagcg gacgcggaac tcgtcggcgc cggcgacgac
     3241 gtgctggttg gtgatgatgt ggc
//
LOCUS       DASUPW010000160         4422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_37147, whole genome shotgun sequence.
ACCESSION   DASUPW010000160 DASUPW010000000
VERSION     DASUPW010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37147"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VFY44_03685"
     CDS             complement(<1..590)
                     /locus_tag="VFY44_03685"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5909567.1"
                     /translation="MTLVLAGTEDHPHARVSLVTALEAGPSHAYLFHGPAGVGKREAA
                     RAFAAELLAQGSPDPDGVRHRVHSGTHPDLTWVRPTGAHVMRRSDVAQAVVDAAGRTP
                     FEASRRVFVLERVDALNDEAANSLLKTLEEPASFVHLILLTDSLGQVMETVVSRCQLV
                     RFDPLPPARIAQALEAEGVEAARALACARLALGNGER"
     gene            complement(587..1186)
                     /gene="tmk"
                     /locus_tag="VFY44_03690"
     CDS             complement(587..1186)
                     /gene="tmk"
                     /locus_tag="VFY44_03690"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX5909568.1"
                     /translation="MPGLFVTLEGIDRSGKSTQAALLADALGDGAVAVREPGGTAAGE
                     RVRDILKDPGVELGPEAEALLFAAARAELVRQVIRPALDKGRTVVCDRFLDSSLAYQG
                     TARDMGLDTVRRINAPAVGDLVPDVTVLLEIDTGAAAGRGAEDDRFEREGDALQRAVA
                     EAYGALADAEPDRFVRVDAARDPEAVHADVLAAVREAGG"
     gene            complement(1188..2207)
                     /gene="galE"
                     /locus_tag="VFY44_03695"
     CDS             complement(1188..2207)
                     /gene="galE"
                     /locus_tag="VFY44_03695"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX5909569.1"
                     /translation="MAWLLTGGAGYIGAHIALAFRQAGTDVVVLDDLSTGYRGYVPDD
                     VDFVDGSVEDADAVARALDTREITGIVHLAGLKYAGVSVEQPLEFFRANVTGTQVLLE
                     AVAERGIANFLFSSSASWYGTPDAEIVDEDTPPAPESPYGQSKVIGEWLLRDVAVGNP
                     GLNHSSLRYFNAVGSGPVELADHSPHNLFPKVFRALSRGEAPLVFGTDYDTPDGSCVR
                     DYVHVVDLADAHVAVAKHLEAGRPAAPAYNVGRGQGSSVLEVVGTILRETGYDVEPEV
                     RDRRPGDPARIVGAVDRIAADLDWHARHDLDDMVSSAWAAWQHQLEVHGGAPPDGARL
                     AIARA"
     gene            complement(2218..>4422)
                     /locus_tag="VFY44_03700"
     CDS             complement(2218..>4422)
                     /locus_tag="VFY44_03700"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX5909570.1"
                     /translation="AFANGSIGIPEESRLQVAVALISLATLGGLLYGSGLRVQASPAA
                     GWGVALLVAFAGWAALSITWSIAPDESWLEANRALAYALVAGLGTVLGSSLPRAAERV
                     GLAVLAIATVIALYALGGKLFPWLHVGGLIDLNHTAGFSRLRAPLDYWNALGLVCVVA
                     VPLAMRVVVELRYRAAVRVAGAMAMVVLLTTLALTYSRGGLIVLVVAAALTVALGPER
                     LRLGAALAAGVAGSVPPILTVFLSDSLTTDGISVDDRTGMGLVLALTLLLGLGIAAVA
                     ALAIGRAGERLRMSPAGARWAKRAALGVAALALVVVLGALAVSDRGVGGTISHEADQF
                     TKPKLDRQNDPARVLRTNSGNRWVWWEEAVRGFEDRPVTGFGAGSFPLTHRHYRESTL
                     EVLQPHSEPLEFLTETGLVGALLALGGLALLIVAAIRGVRVRAPGPERAFACALLAGV
                     VAWSVHIWVDWDWEVPGVTLAVLIVLGVLAATPRDAGGPARQPVGPRPGRGPLMALGA
                     AAAFVVVALAVLPAAATTLTTDAFTAASGGSAAGLRTGEETAALAKRLNPLSLDPVYA
                     QASFAERGNQPRKAARLLVDAVNEQPDNPDAWNRLLRFQALLDDTTAATASLRVLFDL
                     DPVLANRVAVYTALVNYDPARSASATGTPLPERLRRPRRAPADVRDRVTRQFGGGGAQ
                     DTPAPAPAPAPAPTPTPAPTPSPSPTPDRTPAPKPQPKTPAAPEGDPFRLEG"
BASE COUNT          562 a         1687 c         1679 g          494 t
ORIGIN      
        1 cgctcgccgt tgcccagcgc cagccgcgcg caggccagcg cacgcgccgc ctccacgccc
       61 tcggcctcca gcgcctgcgc gatccgcgcc ggcggcagcg ggtcgaagcg caccagctgg
      121 cagcgcgaga ccaccgtctc catgacctgg cccagcgagt cggtcagcag gatcaggtgc
      181 acgaacgagg ccggctcctc cagcgtcttc agcaggctgt tggccgcctc gtcgttcagg
      241 gcgtccacgc gctcgagcac gaacacgcgg cgcgaggcct cgaacggcgt gcgcccggcg
      301 gcgtcgacca cggcctgggc cacgtcggag cggcgcatca cgtgcgcgcc ggtggggcgc
      361 acccaggtca ggtccgggtg ggtgcccgag tggacgcggt ggcggacgcc gtcggggtcg
      421 ggcgagccct gcgccagcag ctcggccgcg aacgcccgcg ccgcctcgcg cttgcccacc
      481 cccgcgggcc cgtggaagag gtacgcgtgc gaggggccgg cctcgagcgc ggtcaccagg
      541 ctcacgcgcg cgtgcggatg gtcctcggtc ccggccagca cgagcgtcat ccgcccgcct
      601 cccgcaccgc ggccagcacg tcggcgtgca cggcctcggg gtcgcgggcg gcgtccacgc
      661 gcacgaaccg gtccggctcg gcgtcggcca gcgcgccgta ggcctcggcc accgcgcgct
      721 ggagcgcgtc gccctcgcgc tcgaaccggt cgtcctcggc cccgcgcccc gcggcggcgc
      781 cggtgtcgat ctccagcagc acggtcacgt cggggaccag gtcgcccacg gccggcgcgt
      841 tgatgcgccg cacggtgtcc agccccatgt cgcgggccgt gccctggtag gccagcgagg
      901 agtcgaggaa gcggtcgcag accacggtcc ggcccttgtc gagcgccggg cggatgacct
      961 ggcgcaccag ctcggcgcgc gcggcggcga acagcagcgc ctcggcctcc gggcccagct
     1021 cgacgcccgg gtccttgagg atgtcgcgca cccgctcgcc ggccgccgtg ccgccgggct
     1081 cgcgcacggc cacggcgccg tcgcccagcg cgtcggccaa cagcgcggcc tgggtgctct
     1141 tgcccgaccg gtcgatgccc tcgagggtga cgaacagccc gggcacgtca ggcgcgcgcg
     1201 atcgccagcc gtgcgccgtc gggcggggcg ccgccgtgga cctcgagctg gtgctgccag
     1261 gcggcccagg cgctggagac catgtcgtcc aggtcgtggc gggcgtgcca gtccaggtcg
     1321 gcggcgatcc ggtccacggc gcccacgatc cgggcggggt cgccgggccg gcggtcgcgc
     1381 acctcgggct ccacgtcgta gcccgtctcg cgcaggatcg tcccgaccac ctcgagcacg
     1441 gacgagccct ggccgcggcc gacgttgtac gcgggagcgg cgggacggcc ggcctccagg
     1501 tgcttggcca ccgccacgtg cgcgtcggcc aggtcgacca cgtgcacgta gtcgcgcacg
     1561 cacgagccgt cgggcgtgtc gtagtcggtg ccgaacacca gcggcgcctc gccgcgcgac
     1621 agcgcgcgga agaccttggg gaacaggttg tgcgggctgt ggtcggccag ctcgaccggg
     1681 cccgagccga ccgcgttgaa gtagcgcagc gaggagtggt tgaggcccgg gttgccgacc
     1741 gccacgtcgc gcagcagcca ctcgccgatg accttgctct ggccgtacgg gctctcgggc
     1801 gccggcgggg tgtcctcgtc cacgatctcg gcgtcgggcg tgccgtacca cgacgcgctg
     1861 ctggagaaca ggaagttggc gatgccccgc tcggccaccg cctccagcag cacctgggtg
     1921 ccggtcacgt tcgcgcggaa gaactccagc ggctgctcga ccgagacccc ggcgtacttc
     1981 agcccggcca ggtggacgat gcccgtgatc tcacgcgtgt ccagcgcgcg ggccaccgcg
     2041 tcggcgtcct cgaccgaccc gtcgacgaag tcgacgtcgt ccggcacgta gccgcggtag
     2101 cccgtggaca ggtcgtccag caccaccacg tcggtgcccg cctggcggaa cgccagggcg
     2161 atgtgggcgc cgatgtatcc ggctccgccg gtgagcagcc aggccatgcg ggggacccta
     2221 gccctcgagc cggaacgggt cgccctcggg ggcggccggt gtcttgggct gcggcttggg
     2281 agccggcgtg cggtccggtg tcgggctggg gctgggcgtc ggagccggcg tcggggtggg
     2341 tgccggcgct ggcgcgggtg ccggcgcggg cgtgtcctgg gcgcccccgc cgccgaactg
     2401 ccgcgtgacc cggtcgcgca cgtcggccgg ggcgcgccgc gggcggcgca ggcgctcggg
     2461 cagcggcgtg cccgtggcgc tggccgagcg ggccgggtcg tagttcacga gcgcggtgta
     2521 gacggccacg cggttggcca gcaccgggtc gaggtcgaag agcacgcgca gcgaggccgt
     2581 ggccgcggtg gtgtcgtcca gcagcgcctg gaagcgcagc aggcggttcc aggcgtccgg
     2641 gttgtcgggc tgctcgttca ccgcgtccac cagcagccgc gcggccttgc gcggttggtt
     2701 gccacgctcg gcgaacgagg cctgcgcgta cacggggtcc agcgagagcg ggttcaggcg
     2761 cttggccagg gccgccgtct cctcgccggt gcgcaacccg gccgcgctgc cgccggacgc
     2821 ggcggtgaag gcgtcggtgg tcagggtcgt tgcggcggcg ggcaggacgg ccagcgcgac
     2881 caccacgaac gcggccgcgg cgcccagcgc catcagcggc ccgcggcccg ggcgcgggcc
     2941 tacgggctgc cgcgcgggtc cgccggcgtc ccgcggcgtc gcggccagga cgccgagcac
     3001 gatcagaacg gccagcgtga cccccgggac ctcccagtcc cagtcgaccc agatgtgcac
     3061 gctccacgcc accaccccgg ccaggagcgc gcacgcgaac gcgcgttcgg ggccgggcgc
     3121 ccggacgcgg acgccgcgga tggcggccac gatgagcagc gccagcccgc cgagcgcgag
     3181 cagcgcgccg accaggcccg tctcggtcag gaactccagc ggctcgctgt ggggctggag
     3241 cacctccagc gtgctctcgc ggtagtggcg gtgggtcagc gggaacgatc cggcgccgaa
     3301 gccggtgacc gggcggtcct cgaagccgcg cacggcctcc tcccaccaca cccagcggtt
     3361 gccggagttg gtgcgcagca cgcgcgcggg atcgttctgg cggtcgagct tgggcttggt
     3421 gaactggtcg gcctcgtgcg agatcgtgcc gcccacgccg cggtcggaca cggccagcgc
     3481 gccgagcacg accacgagcg ccagcgcggc gacgcccagc gcggcgcgct tggcccacct
     3541 ggcccccgcc ggcgacatcc gcagccgctc gcccgcgcgg ccgatggcca gggcggccac
     3601 ggcggcgatg cccagcccca gcagcagcgt cagcgccagc accaggccca tgcccgtgcg
     3661 gtcgtcaacg ctgatcccgt cggtggtcag gctgtcgctc aggaacaccg tcaggatggg
     3721 cggcaccgag ccggcgacgc cggcggccag ggccgcgccc aggcgcaggc gctcgggccc
     3781 cagggcgacg gtcagcgcgg ccgccacgac caggacgatg aggcccccgc gcgagtaggt
     3841 cagcgccagc gtggtcagca gcaccaccat cgccatggcc ccggccaccc gcaccgcggc
     3901 ccggtagcgc agctcgacca ccacgcgcat ggccagcggc accgccacca cgcacaccag
     3961 cccgagcgcg ttccagtagt ccagcggcgc gcgcaggcgc gagaagcccg cggtgtggtt
     4021 caggtcgatc aggccgccga cgtgcagcca ggggaacagc ttgccgccga gcgcgtacag
     4081 cgcgatgacc gtggcgatgg ccagcacggc caggcccacg cgctcggccg cgcgcgggag
     4141 gctggagccg agcacggtgc ccagcccggc caccagcgcg taggccagcg cccggttcgc
     4201 ctcgagccag ctctcgtcgg gggcgatcga ccacgtgatc gacagcgcgg cccagcccgc
     4261 gaacgccacc agcagcgcca cgccccagcc ggccgccggg gaggcctgga cgcgcagccc
     4321 cgagccgtac agcagcccac cgagggtggc cagcgagatc agcgccaccg ccacctggag
     4381 ccggctctcc tcggggatcc cgatcgaccc gttggcgaac gc
//
LOCUS       DASUPW010000161         6644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_176681, whole genome shotgun sequence.
ACCESSION   DASUPW010000161 DASUPW010000000
VERSION     DASUPW010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176681"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VFY44_03705"
     CDS             <1..439
                     /locus_tag="VFY44_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909571.1"
                     /translation="QGNWIPHKLRDATEDGIFFAGDSAGHCLPLTAEGIRTALHFGLV
                     CGDELREVVAGTRTREQALQRYHAFNDEHEWKFRWMLRVQGWVPRLRPRPLRALVAAM
                     GRHQRLTDWAFRHYLRIAAPDRVGPAPKHAPVPDTGERAATAA"
     gene            complement(443..670)
                     /locus_tag="VFY44_03710"
     CDS             complement(443..670)
                     /locus_tag="VFY44_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909572.1"
                     /translation="MNLQTARAALWTLVGAVVLLGIFFFALGGVDPAEAEVMAILIVV
                     LGVLWLIHFWRVRQVTGDIRMQRGDRERRGF"
     gene            complement(691..3312)
                     /gene="polA"
                     /locus_tag="VFY44_03715"
     CDS             complement(691..3312)
                     /gene="polA"
                     /locus_tag="VFY44_03715"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX5909573.1"
                     /translation="MATDGTRELFLIDGNSLVYRAFFALPESIATSRGVPTNAIFGFA
                     SMLVKLMTEHGQKPTVVVWDAGHSGRKDMAPDYKAGRASRPDLLKEQWPALEPLVDAF
                     GYTNVRSDGFEADDVIATLAGRAAREGVDVTIVTGDRDAFQLIEDVEGGGKVRIMATG
                     RGITDTKLYDREAVVDRYGIPPEKIPDFYGLKGDTSDNIPGVPGIGDKTASTLLQEYG
                     DLETVLASVDKISGAKRKENLTNHADDARLSRDLATAQRDVPVELDLDGSLLREPDRS
                     RLRDTFREYELRDPLRRLEEALGDEEAAAPRPEASSSVKAKLKEAAPADLPGMKGDLA
                     VLAAARPEPEEGQLPGFDEGETLRFAAWAGGDHVLAGEAPGGFAELLTGWGDRPVVAH
                     DWKEIASKEDPAVAPPLQHDTVVAAYLLDPARRGYPLRDVAEEQGLGADVSGGEDPLA
                     EEAMLTRALAERQRAELEELGLMRLLNEVELPLVDVLVEMQREGVKLDVPQVRTIAKR
                     VTEQAQELEREIWELAGEEFTIGSPQQLGVILFEKLGLSRKRRGKTGYSTDARVLQAI
                     RSEHEIIGKIETWRELTKLASTYLDAFPDLVGPDSRLRTTFSQVTAATGRLSSTNPNL
                     QNIPIRTELGREIRACFVADEGHRLISADYSQVELRLLAHIAGEDVLKEIFRKGEDVH
                     TATAEQILGGKPDPGTRSKAKMVNYGIVYGLSAYGLADRLQIEQDEAQAFIDAYLDRF
                     PAVKKFIDDTIATATDQGYVTTEFGRIRRIPELRARNYQTRTLGERLAVNMVIQGTAA
                     DIIKVAMVDCRAALRDAGLKTRTVLQIHDELLFEAPEAEVEQASEIVREQMAGAFEMD
                     PPLEVDVADGENWLAAK"
     gene            3378..4826
                     /locus_tag="VFY44_03720"
     CDS             3378..4826
                     /locus_tag="VFY44_03720"
                     /inference="COORDINATES: protein motif:HMM:NF015882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEX5909574.1"
                     /translation="MASPSAQFSLTLRVELPHESGTLSKVTAAITKAGGAIVAVDTVE
                     AGGTGTVREITVDCETAEHRGRVVKAVQGVREAKLVETTDRTFQLHRRGKIYTGLSSP
                     VATRDDLSMAYTPGVARVCSAIAENRQKAFKYTIKANTVAVVTDGTAVLGLGDIGPEA
                     AMPVMEGKAMLFKEFADVDAFPIALDTKDPDKIVETVKLMAPTFGGINLEDISSPRCF
                     EIEERLKAELDIPVFHDDQHGTAIVTLAALINACRLTQRPLESLSVAMAGAGAAGVAV
                     AKILMGAGVTSIVACDRFGAIHSGRQDYEAGSMNAPKTWLAENTNSERRDGSPADVLE
                     GTDLFIGLSGPGIIQARDLQRMNADPFVFAMANPNPEVTPEEAAPYVRVMATGRSDFP
                     NQINNVLAFPGIFRGALDSRAQAITEEMKLAAARGIASTITDDELASDYIIPSVFNRE
                     VSRKVAQAVAEEAERTGATQSLADTAEFSIPG"
     gene            4839..5759
                     /locus_tag="VFY44_03725"
     CDS             4839..5759
                     /locus_tag="VFY44_03725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEX5909575.1"
                     /translation="MKVTVTGATGTLGREVVAELKGRGDHVTVLSRDADRARQTLGQE
                     VTALEWADPKSQAPPAEAFAGQDAVVHLLGEPIAQRWSDDTKREIRDSRVLTTRNLVA
                     VLRDLDPAERPEVLVSQSAVGWYGARGDEKVDESEPVADDFLAQVCKDWEAEAQAAEE
                     LGLRVVRTRTGVVLSEDGGALEKMLPPFKLGVGGPVAGGKQYVPWVHTDDVVGSLLHC
                     LDQGSVAGPVNVTAPEPVTNAELSKALGRVLHRPAFAPVPGLAVKLLYGEMAIIVTTG
                     SRAVPTALERSGYAFRQADLEAALRAATGR"
     gene            complement(5756..6145)
                     /locus_tag="VFY44_03730"
     CDS             complement(5756..6145)
                     /locus_tag="VFY44_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909576.1"
                     /translation="MSLTLGQVVVPSEGLDSGLYMPLYLVLILYVVLFVTGRVFEGRD
                     DPRTETVLDAGFALMCLAAVYVAILLVYAFASEFDLIVDMVEIMAIMIGFFALLVVAL
                     MGIELLFGLGGRRRRKGVEVPPPEKTD"
     gene            complement(6142..6414)
                     /locus_tag="VFY44_03735"
     CDS             complement(6142..6414)
                     /locus_tag="VFY44_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909577.1"
                     /translation="MTVFALLGAKSLYLLFVWLISAIVASYLSDRKGYGEKPGLVTGM
                     LLSVLAIVIWLVFPARKDSRWKLQGAFGRGDGRTVAQLRAEQEDSP"
     gene            complement(6411..>6644)
                     /locus_tag="VFY44_03740"
     CDS             complement(6411..>6644)
                     /locus_tag="VFY44_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5909578.1"
                     /translation="LDEPSMGIAPILVERIYETIAQINEQGMTILLVEQNANFALGVS
                     KRGYVLETGEVALSDEADKLRDNPEVQKAYLGA"
BASE COUNT          975 a         2380 c         2356 g          933 t
ORIGIN      
        1 ccagggcaac tggatcccgc acaagctgcg cgacgcgacc gaggacggca tcttcttcgc
       61 cggcgactcg gccgggcact gccttccgct gaccgcggag ggcatccgca ccgcgctgca
      121 cttcggcctg gtgtgcggcg acgagctgcg cgaggtggtc gccggcacgc ggacccgcga
      181 gcaggcgctg cagcgctacc acgccttcaa cgacgagcac gagtggaagt tccgctggat
      241 gctgcgcgtg cagggctggg tgccgcgcct gcggccgcgc ccgttgcggg ccctggtggc
      301 cgcgatgggc cgccaccagc ggctgaccga ctgggccttc cggcactacc tgcggatcgc
      361 ggcgcccgac cgcgtcggcc ccgcccccaa gcacgcgccg gtgcccgaca ccggcgagcg
      421 cgcggctacc gccgcctgag cctcagaagc cgcgccgctc gcggtcgccg cgctgcatcc
      481 ggatgtcccc ggtgacctgg cgcacccgcc agaagtggat cagccacagg acgcccagca
      541 ccacgatcag gatcgccatg acctcggcct cggccgggtc gaccccgccg agcgcgaaga
      601 agaagatgcc cagcaggacc acggcgccca ccagcgtcca gagcgcagcc cgtgccgtct
      661 gcaggttcat ggcgcgaaaa tggtaccgcg ctacttcgcg gcgagccagt tctcgccgtc
      721 ggccacgtcg acctccagcg gggggtccat ctcgaacgcg cccgccatct gctcgcggac
      781 gatctccgac gcctgctcga cctcggcctc cggcgcctcg aacagcagct cgtcgtggat
      841 ctggagcacg gtgcgtgtct tcagcccggc gtcgcgcagc gcggcgcggc agtcgaccat
      901 ggccaccttg atgatgtcgg cggcggtgcc ctggatgacc atgttcaccg ccagccgctc
      961 gcccagcgtg cgggtctggt agttgcgggc ccgcagctcc gggatccgcc ggatgcggcc
     1021 gaactcggtg gtcacgtagc cctggtcggt tgccgtggcg atggtgtcgt cgatgaactt
     1081 cttgaccgcc gggaagcggt ccaggtacgc gtcgatgaag gcctgcgcct cgtcctgctc
     1141 gatctggagc cggtcggcca ggccgtacgc cgagagcccg tagacgatgc cgtagttgac
     1201 catcttggcc ttcgagcgcg tgccgggatc gggcttgccg cccaggatct gctcggcggt
     1261 ggcggtgtgc acgtcctcgc ccttgcggaa gatctccttg agcacgtcct cgccggcgat
     1321 gtgggccagc agccgcagct ccacctggga gtagtcggcg ctgatcagcc ggtggccctc
     1381 gtcggccacg aagcaggccc ggatctcgcg ccccagctcg gtgcggatgg ggatgttctg
     1441 caggttgggg ttggtcgacg agagccggcc ggtggcggcg gtgacctggc tgaaggtggt
     1501 gcgcaggcgg ctgtccgggc ccacgaggtc ggggaaggcg tccaggtagg tcgaggccag
     1561 cttggtcagc tcgcgccagg tctcgatctt cccgatgatc tcgtgctcgg agcggatggc
     1621 ctggagcacc cgggcgtcgg tggagtagcc ggtcttgccg cgccgcttgc gcgacaggcc
     1681 gagcttctcg aacaggatca cgccgagctg ctggggcgag ccgatcgtga actcctcccc
     1741 cgccagctcc cagatctcgc gctcgagctc ctgggcctgc tcggtcacgc gcttggcgat
     1801 ggtgcgcacc tgcggcacgt cgagcttgac gccctcgcgc tgcatctcga cgaggacgtc
     1861 gaccagcggc agctcgacct cgttcagcag ccgcatcagg cccagctcct cgagctcggc
     1921 gcgctggcgc tcggccaggg cgcgggtgag catggcctcc tcggccagcg ggtcctcgcc
     1981 cccggagacg tcggcgccca ggccctgctc ctcggccacg tcgcggagcg ggtagccccg
     2041 ccgcgccgga tccaggaggt aggcggccac gaccgtgtcg tgctgcagcg gcggcgccac
     2101 cgccgggtcc tccttggagg cgatctcctt ccagtcgtgc gccaccaccg gccggtcgcc
     2161 ccagccggtc agcagctcgg cgaacccgcc gggcgcctcg ccggccagca cgtggtcgcc
     2221 gccggcccag gcggcgaagc gcagcgtctc gccctcgtcg aagcccggca gctggccctc
     2281 ctcgggctcg ggccgcgcgg cggccaggac ggccaggtcg cccttcatcc cgggcaggtc
     2341 ggccggagcg gcctctttga gcttggcctt gaccgacgac gaggcctcgg gccggggcgc
     2401 ggcggcctcc tcgtccccca gcgcctcctc gaggcggcgc aacgggtcgc gcagctcgta
     2461 ctcgcggaag gtgtcgcgca ggcgcgagcg gtcgggctcg cgcagcagcg agccgtccag
     2521 gtcgagctcc acgggcacgt cccgctgggc ggtggccagg tcgcgcgaca ggcgcgcgtc
     2581 gtcggcgtgg ttggtcaggt tctccttgcg cttggcgccc gagatcttgt ccaccgaggc
     2641 caggaccgtc tccaggtcgc cgtactcctg cagcagcgtg gaggcggtct tgtcgccgat
     2701 gccggggacg ccggggatgt tgtccgaggt gtcgcccttg agcccgtaga aatcggggat
     2761 cttctccggc gggatgccgt accggtccac caccgcctcg cggtcgtaga gcttggtgtc
     2821 ggtgatcccg cggccggtgg ccatgatccg caccttgccg ccgccctcca cgtcctcgat
     2881 cagctggaac gcgtcgcggt cgccggtcac gatcgtcacg tcgacgccct cgcgcgcggc
     2941 gcgcccggcc agcgtggcga tcacgtcgtc ggcctcgaac ccgtccgagc gcacgttggt
     3001 gtagccgaac gcgtccacca gcggctccag cgcgggccac tgctccttga gcaggtcggg
     3061 gcgcgacgcg cggcccgcct tgtagtcggg ggccatgtcc ttgcggcccg agtggccggc
     3121 gtcccacacc acgacggtcg gcttctgacc gtgctcggtc atcagcttca ccagcatcga
     3181 ggcgaagccg aagatggcgt tcgtcggcac gccgcgcgag gtggcgatcg attccgggag
     3241 cgcgaagaac gcgcggtaga ccagcgagtt gccgtcgatc aggaagagct cgcgggtgcc
     3301 gtcggtggcc acgggcccat cctacgaggc gtaccggggc acggccctgc gcccgaatat
     3361 gatcgggtac cccgccgatg gcctccccca gcgcacagtt ctccctgacc ctccgggtcg
     3421 agctccccca cgagtcggga acgctgagca aggtcaccgc cgccatcacc aaggcgggcg
     3481 gggccatcgt ggccgtcgac accgtcgagg ccggcggcac cggcaccgtg cgcgagatca
     3541 ccgtggactg cgagacggcc gagcaccgcg ggcgcgtggt caaggccgtg cagggcgtcc
     3601 gcgaggccaa gctggtcgag accaccgacc gcacgttcca gctgcaccgg cggggcaaga
     3661 tctacacggg gctgtcgtcg cccgtggcca cgcgcgacga cctgtcgatg gcctacacgc
     3721 cgggcgtcgc gcgcgtgtgc agcgccatcg ccgagaaccg ccagaaggcc ttcaagtaca
     3781 cgatcaaggc gaacacggtg gccgtggtca ccgacggcac cgccgtgctg ggcctgggcg
     3841 acatcggccc cgaggcggcc atgccggtca tggagggcaa ggcgatgctg ttcaaggagt
     3901 tcgccgacgt ggacgccttc ccgatcgcgc tggacaccaa ggacccggac aagatcgtgg
     3961 agaccgtgaa gctgatggcc cccacgttcg gcggcatcaa cctggaggac atcagctcgc
     4021 cgcgctgctt cgagatcgag gagcggctga aggcggagct ggacatcccg gtgttccacg
     4081 acgaccagca cgggaccgcg atcgtcacgc tggccgccct gatcaacgcc tgccggctga
     4141 cccagcgccc gctggagagc ctgtcggtgg ccatggcggg cgcgggcgcc gccggggtcg
     4201 ccgtggccaa gatcctgatg ggcgcgggcg tgaccagcat cgtggcctgt gaccgcttcg
     4261 gcgcgatcca ctccggccgc caggactacg aggcggggtc gatgaacgcg cccaagacct
     4321 ggctcgcgga gaacaccaac tccgagcgcc gcgacggctc accggccgac gtgctggagg
     4381 gcaccgacct gttcatcggc ctgtccgggc ccgggatcat ccaggcccgc gacctgcagc
     4441 ggatgaacgc cgacccgttc gtgttcgcca tggccaaccc caaccccgag gtcacgcccg
     4501 aggaggccgc cccctacgtg cgcgtcatgg ccacgggccg ctcggacttc cccaaccaga
     4561 tcaacaacgt gctggccttc ccggggatct tccgcggcgc cctggactcg cgcgcgcagg
     4621 ccatcaccga ggagatgaag ctggccgcgg cccgcgggat cgcctcgacg atcaccgacg
     4681 acgagctggc ctcggactac atcatcccca gcgtcttcaa ccgcgaggtg agccgaaagg
     4741 tggctcaggc cgtagccgaa gaagcggaac ggaccggcgc cacccagtcg ctggccgaca
     4801 ccgcggaatt ctcgataccc ggctagagga gtcatcacgt gaaggtcacc gtcacgggcg
     4861 ccaccggcac cctgggacgc gaggtcgtgg ccgagctgaa gggccgcggc gaccacgtca
     4921 cggtgctctc gcgcgacgcc gaccgcgccc gccagacgct ggggcaggag gtgaccgcgc
     4981 tggagtgggc cgaccccaag tcccaggcac ccccggccga ggcgttcgcc ggccaggacg
     5041 cggtcgtcca cctgctgggc gagccgatcg cccagcgctg gagcgacgac accaagcgcg
     5101 agatccgcga ctcgcgggtg ctgaccaccc gcaacctggt ggccgtcctg cgtgacctcg
     5161 acccggccga gcggccggag gtgctggtct cccagtcggc ggtgggctgg tacggcgccc
     5221 gcggcgacga gaaggtggac gagtccgagc cggtggccga cgacttcctg gcccaggtct
     5281 gcaaggactg ggaggccgag gcgcaggccg ccgaggagct ggggctgcgc gtggtgcgca
     5341 cccgcaccgg cgtggtgctg tccgaggacg gcggcgcgct ggagaagatg ctgcccccgt
     5401 tcaagctcgg cgtcggcggg ccggtggcgg gcgggaagca gtacgtgccc tgggtccaca
     5461 ccgacgacgt ggtcggatcg ctgctgcact gcctggacca gggctcggtg gccgggccgg
     5521 tcaacgtgac cgcgccggag ccggtgacca acgccgagct gtccaaggcg ctgggccgcg
     5581 tgctgcaccg cccggcgttc gcgccggtgc cgggcctggc ggtcaagctg ctctacggcg
     5641 agatggccat catcgtgacc accggctcgc gggccgtgcc gaccgcgctg gaacggtcgg
     5701 gctacgcgtt ccgccaggcc gacctggaag ccgcgctgcg agcggcgacc gggcgctagt
     5761 cggtcttctc gggcggcggg acctcgacgc ccttgcgccg ccggcgcccg ccgagcccga
     5821 agagcagctc gatgcccatc agcgccacca cgagcagggc gaagaagccg atcatgatgg
     5881 ccatgatctc gaccatgtcc acgatcaggt cgaactcgga cgcgaacgcg tagaccagca
     5941 ggatcgccac gtacacggcg gccaggcaca tgagcgcgaa cccggcgtcc agcaccgtct
     6001 ccgtccgcgg atcgtcgcgg ccctcgaaca cccggcccgt gacgaacagg accacgtaga
     6061 ggatcagcac caggtagagg ggcatgtaga gccccgagtc cagtccctcg gagggcacca
     6121 cgacctgtcc cagggtgagg ctcacggcga atcctcctgc tcggcgcgca gctgggcgac
     6181 cgtgcggccg tcaccgcggc cgaacgcgcc ctgaagcttc cagcgcgagt ccttgcgggc
     6241 cgggaacacg agccagatga cgatggccag gacgctcagc agcatcccgg tgaccaggcc
     6301 gggcttctcg ccgtagccct tgcggtcgga caggtaggag gccacgatgg ccgagatcag
     6361 ccacacgaac agcaggtaca gggacttggc gcccaggagc gcgaagacgg tcatgcgccc
     6421 aggtacgcct tctggacctc ggggttgtca cgcagcttgt cggcctcgtc gctgagggcg
     6481 acctcaccgg tctcgagcac gtacccgcgc ttgctgacgc cgagcgcgaa gttcgcgttc
     6541 tgctcgacca gcaggatggt catgccctgc tcgttgatct gggcgatggt ctcgtagatg
     6601 cgctccacca ggatcggggc gatgcccatc gagggctcgt ccag
//
LOCUS       DASUPW010000162         2641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_730402, whole genome shotgun sequence.
ACCESSION   DASUPW010000162 DASUPW010000000
VERSION     DASUPW010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730402"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1327
                     /locus_tag="VFY44_03745"
     CDS             <1..1327
                     /locus_tag="VFY44_03745"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEX5909579.1"
                     /translation="ARNHHPHLELMLAVPTTGAVLNPVNTRLTADQVAWILNHSMARV
                     LFADAELVGLLDPVRDRLEYLERVIVIDDEYEDLLAGAEPDHSPVAAAENDALALCYT
                     SGTAGDPKGVLFSHRSTILHALSLTMVDNHAVSRADTVMPITAVCHVLAWSLPYAAAL
                     TGADLVLQGPSNDPRHLAALIESEGVTTGAAVPTVWLDMEPLFDGDEFDLSSMRELLI
                     GGMPVPKPLIRRYRERGITIAQGWGMTEMSPSGTMSRQTGDGEGASNQGTAMPLVELR
                     LMGDDGAELDWDGATVGELEVRGPCVIAEYYEPTEDASDRFRDGWLRTGDLARIDPDG
                     TVEIVDRAKDLVKSGGEWISSVELEGAIAAHPAVLEAAVVAVPDQKWGERPLALVVVD
                     TALEQPELAGFLEERVARWWIPERVELVDEIPKTSVGKYDKRRIRELYA"
     gene            1324..1806
                     /locus_tag="VFY44_03750"
     CDS             1324..1806
                     /locus_tag="VFY44_03750"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX5909580.1"
                     /translation="MSDDWAFASPPKPWSEAFSDDVAFLFSKIGARANKLYAELLAPL
                     DLRPNQVAVLQYLAASEGASQREIVSGLWIDAGSLVAMLDLFEERGLAERRRNPSDRR
                     ASAVHLTGRGREQLARALELSKEVERRLLGPLPVAERRQLHALLKSITGLNGAGAGPV
                     "
     gene            1820..>2641
                     /locus_tag="VFY44_03755"
     CDS             1820..>2641
                     /locus_tag="VFY44_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5909581.1"
                     /translation="MSSLPNLSSIEFWAQPADERERGFRRLRDEAPLSWQDQPETRLL
                     PAEDGSGGYWAATRFEDIQEVSRDTERFVSGRGVMLEDVPQEFLDAAQSFLAMDGDRH
                     RNLRKLVSSGFTPRQVHHLEDGIRSDVGQILDSLAGHEQGDFVELVAKQLPLMTIMRM
                     LGVPEHDRERLVHQADAMVSWNDPDYIGEREPLEVIGTAIATLHQACIEYCEQRREQP
                     GDDLISALVQAEVDGERLSNEQIASFFVLLSVAGNDTTRHTTSHTMRQLTLHPDQR"
BASE COUNT          391 a          949 c          944 g          357 t
ORIGIN      
        1 cgcgcgcaac caccatcccc acctggagct gatgctggcc gtgccgacca cgggcgcggt
       61 gctgaacccg gtgaacaccc gcctgaccgc cgaccaggtg gcctggatcc tcaaccactc
      121 catggcccgg gtgctgttcg ccgacgccga gctggtcggc ctgctggacc cggtccgcga
      181 ccggctcgag tacctcgagc gggtgatcgt catcgacgac gagtacgagg acctgctggc
      241 cggcgccgag cccgaccact caccggtggc agcggccgag aacgacgcgc tcgccctctg
      301 ttacacgtcg ggcacggccg gcgaccccaa gggcgtgctg ttctcgcacc gctcgacgat
      361 cctgcacgcg ctgtcgctga cgatggtcga caaccacgcc gtctcccgcg cggacaccgt
      421 catgcccatc accgccgtct gccacgtgct ggcgtggtcg ctgccctacg ccgccgcgct
      481 caccggtgcc gacctggtgc tccaggggcc gtcgaacgac ccgcgccacc tggccgccct
      541 gatcgagtca gagggcgtga ccaccggcgc cgccgtgccc acggtgtggc tggacatgga
      601 gccgctgttc gacggcgacg agttcgacct gtcctcgatg cgcgagctgc tcatcggcgg
      661 catgccggtg cccaagccgc tgatccgccg ctaccgcgag cgcgggatca cgatcgccca
      721 gggctggggc atgaccgaga tgtcgcccag cgggaccatg tcgcgccaga ccggcgacgg
      781 cgagggggca tccaaccagg gcaccgcgat gccgctggtc gagctgcgcc tgatgggcga
      841 cgacggcgcc gagctggact gggacggcgc caccgtcggc gagctggagg tacgcggccc
      901 gtgcgtgatc gccgagtact acgagcccac cgaggacgcc tcggaccgct tccgcgacgg
      961 ctggctgcgc accggcgacc tggcccggat cgaccccgac ggcacggtcg agatcgtcga
     1021 ccgggccaag gacctggtca agtccggcgg cgagtggatc agctcggtgg agctggaggg
     1081 cgccatcgcc gcccacccgg cggtcctgga ggccgcggtg gtggcggtgc ccgaccagaa
     1141 gtggggcgag cggccgctgg ccctggtggt ggtggacacg gcgctcgagc agcccgagct
     1201 ggccgggttc ctggaagagc gcgtggcccg ctggtggatc cccgagcgcg tcgagctggt
     1261 ggacgagatc ccgaagacgt ccgtcggcaa gtacgacaag cggcgcatcc gcgagctcta
     1321 cgcatgagcg acgactgggc cttcgcctcg ccccccaagc cctggtccga ggcgttctcc
     1381 gacgacgtgg ccttcctgtt ctcgaagatc ggcgcgcgcg cgaacaagct ctacgccgag
     1441 ctgctggccc cgctggacct gcgaccgaac caggtcgcgg tgctgcagta cctggcagcg
     1501 tccgagggcg cgtcgcagcg cgagatcgtc agcggcctct ggatcgacgc cggctccctg
     1561 gtggccatgc tcgacctgtt cgaggagcgc ggcctggccg agcgccggcg caaccccagc
     1621 gaccggcgcg cctcggcggt gcacctgacc ggccgaggcc gcgagcagct ggcgcgggcg
     1681 ctggagctgt cgaaggaggt cgagcgccgc ctgctcggtc cgctgccggt ggccgagcgc
     1741 cggcagctgc acgcgctgct gaagtccatc acgggattga acggcgcggg cgccggcccg
     1801 gtgtaggacc attttggccg tgtcctcgct gccgaacctg tccagcatcg agttctgggc
     1861 ccagccggcc gacgagcgcg aacgcggctt ccggcgcctg cgcgacgagg cgccgctgtc
     1921 gtggcaggac cagcccgaga cgcggctcct gccggccgag gacggcagcg gcggctactg
     1981 ggccgccacc cgcttcgagg acatccagga ggtctcgcgc gacaccgagc ggttcgtctc
     2041 cggccggggg gtgatgctgg aggacgtgcc ccaggagttc ctggacgcgg cccagtcgtt
     2101 cctggccatg gacggcgacc gccaccgcaa cctccgcaag ctggtgtcca gcggcttcac
     2161 cccgcgtcag gtgcaccacc tcgaggacgg gatcaggagc gacgtgggcc agatcctgga
     2221 ttcgctggcc gggcacgagc agggcgactt cgtggagctg gtggccaagc agctgccgct
     2281 gatgacgatc atgcggatgc tgggcgtgcc cgagcacgac cgtgagcgcc tggtccacca
     2341 ggccgacgcg atggtgtcct ggaacgaccc cgactacatc ggcgagcgcg agccgctgga
     2401 ggtcatcggc accgccatcg ccacgctgca ccaggcctgc atcgagtact gcgagcagcg
     2461 ccgcgagcag ccgggtgacg acctgatcag cgcgctggtg caggccgagg tcgacggcga
     2521 gcgcctgagc aacgagcaga tcgcctcctt cttcgtgctg ctgtcggtgg cgggcaacga
     2581 cacgacgcgc cacacgacgt cgcacacgat gcgccagctc acgctgcacc ccgaccagcg
     2641 g
//
LOCUS       DASUPW010000163         2964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1977682, whole genome shotgun sequence.
ACCESSION   DASUPW010000163 DASUPW010000000
VERSION     DASUPW010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1977682"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="VFY44_03760"
     CDS             <1..846
                     /locus_tag="VFY44_03760"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5909582.1"
                     /translation="RELLTDPEIDVVSVASYDDAHFDQIRTALEHGKHVFAEKPLVLY
                     ADQARQIAGLLAERPDLVLSTNVPLRRSPRFVRLRDRIQAGELGRLFLMEADYEYGRR
                     HKLEQGWRADIPYYSAFLGGGIHMVDLLLWLTGERVTEVLGAHANRIATEGSGFKHPD
                     LVTATMRTETGALMKVNANLGSVGPHFHAVRLYGTDATFVNGLPDGVLHRPEGVSEVV
                     DDPYPGVGKEVLIGSFLDAILTGEPAQVTARDVFDTLSVCMAVQHALETGETVPVEDL
                     LPSTG"
     gene            846..1997
                     /locus_tag="VFY44_03765"
     CDS             846..1997
                     /locus_tag="VFY44_03765"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX5909583.1"
                     /translation="MATQQMIHFGRPMVGDREREAVADVLSGTTLTHGPKVKEFEAAF
                     AEFTGAPHAIAVATCMAALHLSYLAIDLGPGDEVIVPAQTHVATAHAVELCGAKPVFL
                     DCGPRTGNLDLDQLEGLITERTRAIGLVHYLGLPLDMDRLMEVARRHDLYVVEDAAVA
                     LGATVDGTHVGLVGDVGCFSFYPVKHITTGEGGMVITNRDDLADRISKQRAFGIDKSV
                     LADRRHTGAYEIEHVGLNYRMGETGAAMGVVQMERLPGFLEHRERIFGILEQGLAEVD
                     ALDLVSSNHQGNRRASHYCLVARLADDGADREQVIERLKSAGVGTSVYYPKALPDTAF
                     YAQRYGYAPGSCPNATRISTRSLAFPCGPHVTEADAERIVQTVKETLST"
     gene            1994..>2964
                     /locus_tag="VFY44_03770"
     CDS             1994..>2964
                     /locus_tag="VFY44_03770"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5909584.1"
                     /translation="MSDLTDQRIALIGGAGFIGHNLALELKSRGAEVAAIDGLEVNHL
                     VHYAASSDPNRDLYIKILLERLRLLEDAGIPLMRQDARDYNGLSNALNEFQPTSIVHL
                     AAVAHANRSNKDPFSTFDHSMRTLENALDWSRGSGLERFVFFSSSMVYGNFETEEITE
                     DHPLNPIGIYGALKLGGEKLVIAYNQVFDLPFTILRPSALYGPRCVSRRVSQAFIESA
                     LVGNTLRVDGEGEERVDFTYIDDVVNGVIASLTHDGARNEIFNMTAGKGRSLKDLVTL
                     VQDHFPEIEVEYVERDNLRPFRGTLLMDKAAEKMGHRPATELEDGLS"
BASE COUNT          489 a         1054 c         1001 g          420 t
ORIGIN      
        1 cgggagctgc tcaccgaccc ggagatcgac gtggtgtcgg tggcctccta cgacgacgcc
       61 cacttcgacc agatccgcac ggcgctggag cacggcaagc acgtgttcgc cgagaagccg
      121 ctggtgctct acgccgacca ggcgcgccag atcgccggcc tgctggccga gcgccccgac
      181 ctggtgctgt ccaccaacgt gcccctgcgg cgcagcccgc gcttcgtgcg gctgcgcgac
      241 cggatccagg ccggcgagct gggccggctc ttcctgatgg aggccgacta cgagtacggg
      301 cgcaggcaca agctcgagca gggctggagg gccgacatcc cctactactc ggcattcctg
      361 ggcggcggga tccacatggt cgacctgctg ttgtggctga ccggggagcg cgtgaccgag
      421 gtgctgggcg cgcacgcgaa ccgcatcgcc accgagggat ccgggttcaa gcacccggac
      481 ctggtgaccg cgacgatgag aaccgagacg ggcgcgctga tgaaggtgaa cgccaacctg
      541 ggcagcgtcg gcccgcactt ccacgccgtg cgcctctacg gcaccgacgc caccttcgtc
      601 aacgggctgc cggacggcgt cctgcaccgc cccgaggggg tctccgaggt ggtggacgat
      661 ccctacccgg gggtgggcaa ggaggtcctg atcggctcgt tcctggacgc gatcctgacc
      721 ggcgagccgg cccaggtgac cgcccgcgac gtgttcgaca cgctgtcggt gtgcatggcc
      781 gtccagcacg cgctggagac cggggagacg gttcccgtcg aggatctcct accctcgaca
      841 ggctgatggc cacccagcag atgatccatt tcggccggcc catggtcggc gaccgcgagc
      901 gcgaggccgt ggccgacgtg ctgtcgggca ccacgctcac ccacggcccg aaggtcaagg
      961 agttcgaggc ggcgttcgcc gagttcaccg gcgcgcccca cgccatcgcg gtggccacct
     1021 gcatggccgc gctgcacctg agctacctgg ccatcgacct ggggcccggg gacgaggtca
     1081 tcgtcccggc gcagacgcac gtggccaccg cgcacgccgt ggagctgtgc ggcgccaagc
     1141 ccgtgttcct ggactgcggg ccgcgcacgg gcaacctcga cctggaccag ctcgagggcc
     1201 tgatcaccga gcgcacccgc gccatcggcc tcgtgcacta cctgggcctg cccctggaca
     1261 tggaccgcct gatggaggtg gcccgcaggc acgacctgta cgtggtcgag gacgccgcgg
     1321 tggccctggg cgcgaccgtg gacggcaccc acgtgggcct ggtcggcgac gtcggctgct
     1381 tttcgttcta ccccgtcaag cacatcacca ccggcgaggg gggaatggtc attaccaatc
     1441 gcgacgacct ggccgaccgg atctccaagc agcgggcgtt cgggatcgac aagagcgtgc
     1501 tggccgaccg ccgccacacc ggcgcctacg agatcgagca cgtgggcctg aactaccgca
     1561 tgggcgagac cggcgccgcc atgggcgtcg tgcagatgga acggctgccg gggttcctcg
     1621 agcaccgcga gcggatcttc gggatcctcg agcagggcct ggccgaggtg gatgcgctcg
     1681 acctcgtgag ctcgaaccac cagggcaacc gccgggccag ccactactgc ctggtcgccc
     1741 gcctggccga cgacggcgcc gaccgcgagc aggtgatcga gcgtctgaag tccgccggtg
     1801 tgggtaccag cgtctactac cccaaggcgt tgccggacac cgcgttctac gcgcagaggt
     1861 acggctacgc tcccggatcc tgtccgaacg cgaccaggat cagcacccgc tccctggcct
     1921 tcccgtgcgg cccgcacgtg accgaggccg acgccgagcg gatcgtccag accgtgaagg
     1981 agaccctcag cacatgagcg acctgaccga ccagcgcata gccctcatcg ggggcgccgg
     2041 gttcatcggc cacaacctgg ccctggagct caagagccgt ggcgcggagg tggccgccat
     2101 cgacggcctc gaggtcaacc acctggtgca ctacgccgcc tcctcggacc ccaaccgcga
     2161 cctgtacatc aagatcctgc tggagcggct gaggctgctg gaggacgccg gcatcccgct
     2221 gatgcgccag gacgcccgcg actacaacgg gctgtcgaac gccctgaacg agttccagcc
     2281 cacgtcgatc gtccacctgg cggccgtggc gcacgccaac cgctcgaaca aggacccgtt
     2341 ctcgaccttc gaccactcga tgcgcacgct cgagaacgcg ctcgactggt cgcgcggctc
     2401 ggggctcgag cggttcgtct tcttctccag ctccatggtc tacggcaact tcgagaccga
     2461 ggagatcacc gaggaccacc cgctgaaccc gatcgggatc tacggcgccc tgaagctggg
     2521 cggcgagaag ctggtgatcg cctacaacca ggtcttcgac ctgccgttca cgatcctgcg
     2581 cccatcggcc ctgtacggcc cgcgatgcgt gtcgcgccgc gtcagccagg cgttcatcga
     2641 gagcgcgctg gtgggcaaca ccctgcgcgt ggacggcgag ggcgaggagc gggtggactt
     2701 cacctacatc gacgacgtgg tcaacggcgt gatcgcctcg ctgacccacg acggggcccg
     2761 caacgagatc ttcaacatga ccgccggcaa gggccgctcg ctgaaggacc tggtcacgct
     2821 ggtccaggac cacttccccg agatcgaggt cgagtacgtg gagcgcgaca acctgcgccc
     2881 gttccgcggc acgctgctca tggacaaggc ggcggagaag atgggccatc gcccggcgac
     2941 cgagctggag gacggcctgt ccaa
//
LOCUS       DASUPW010000164         3496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1423554, whole genome shotgun sequence.
ACCESSION   DASUPW010000164 DASUPW010000000
VERSION     DASUPW010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423554"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /locus_tag="VFY44_03775"
     CDS             complement(<1..1258)
                     /locus_tag="VFY44_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909585.1"
                     /translation="MRVAGAIAAAVAVLLAGAGSAHAFDPVREAANVAKSGEREQVWL
                     QPGYQVQLGLESQRTQTEAALTAARDPERNFLTDLCNSKGLACAGDIRLNDWKANGYG
                     LVERVLWTARNGSTVAGRVWATRAGARKRPAIVITNGSIQASEELYWYAAQTLAKAGY
                     VVVTFDPQMQGLSDTYGEGGDRNEGFPSQTTGNTFYDWTQDAIDFLLSSPAKPFCARA
                     SRSGTSHCAKQDRRVKAGLNAAYNPMWKLVEGHRIGIAGHSYGASGVSWMGQQDKRVD
                     AVVAWDNLCDPSLRVGQRGDSPTAGATCSSGAQGPPPGHRVPSLGLSADSFLGETRAS
                     DPDPLAKSAGSRSFTKGGVDTGSITIRGGTHFEFSYIPMQPFKATLRGIDLTTWYTRA
                     WFDRYVKEDPAADRQLLSARWRSDPGD"
     gene            complement(1302..1940)
                     /locus_tag="VFY44_03780"
     CDS             complement(1302..1940)
                     /locus_tag="VFY44_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909586.1"
                     /translation="MTLSPRFTAISWLVAGVITLAGMLATPWESSTETADYLATIGSN
                     ATQGQIAALLLHFGYMALVPAFFGVYWLCREHGGRLRAAGGALAVFGAIGLPGLLAVD
                     FYGIALYETLSLKEAVKVEEAVADMPGSAILAMSAVLPMMVGGVLLFVAAYRARVLPL
                     WMPIVLALTMFGPMALEPVFAIAMGAGVLAIGVGMAVNWLRPGVAEPVLTTA"
     gene            complement(2004..>3496)
                     /locus_tag="VFY44_03785"
     CDS             complement(2004..>3496)
                     /locus_tag="VFY44_03785"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_function: GO:0003997 - acyl-CoA oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX5909587.1"
                     /translation="NVQELRTTATYDPDTEEFVIHTPDDWARKEWIGNAACDGVLAAV
                     FCQLVVGGQERGVHALVVPIRDEKGKPAEGVRIEDIGPKLGLDGVDNGRLWFDQVRVP
                     RENLLDRYASVSPEGGYTSPIEKPTKRFFTMLSTLIQGRISVCGAAINASKVALTIAV
                     RHGNARRQFGPPGGDEVTLMTYRTHRRRLLIPLAENYALHFAQERLVSGLEDVFGEDG
                     ADDETRRELETMAAALKAVGTWHATDTIQGCREACGGQGYLKANRFAALKADTDVFTT
                     FEGDNTVLLQLAAKNLLTDFQHEFSELDPFGTFAFVAEQVAGTVAERTAVRELLGRIG
                     DDLLPGRDDEGDLLERETQLEFFRWREQHVKSAAARRLKGGMDAGADPFEVLNDCQDH
                     VVLVGRVYAERVVMEAFADAVERCPDPALQAVLARLCDLYALSRVEADRGWFQEHGKL
                     SSTRSKAVLKGVNTACDELAEHAALLVEAYGVPDAALGDAREIQGN"
BASE COUNT          469 a         1279 c         1244 g          504 t
ORIGIN      
        1 ggtcgccggg atcgctgcgc cagcgggcgg agagcagctg gcggtccgcg gccgggtcct
       61 ccttgacgta gcggtcgaac caggcgcggg tgtaccacgt ggtcagatcg atgccgcgca
      121 gcgtggcctt gaacggctgc atcgggatgt aggagaactc gaagtgggtg ccgccgcgga
      181 tggtgatcga gccggtgtcc acgccgccct tggtgaacga ccgcgagccc gcgctcttgg
      241 ccagcgggtc ggggtcgctg gcccgcgtct cgcccaggaa cgagtccgcg ctcagcccca
      301 gcgacggcac gcggtggccg gggggcgggc cctgcgcgcc cgacgagcag gtggcgccgg
      361 cggtgggcga gtcgccccgc tgcccgaccc gcagcgacgg gtcgcacagg ttgtcccagg
      421 ccacgaccgc gtccacgcgc ttgtcctgct ggcccatcca gctgacgccc gaggcgccgt
      481 aggagtggcc ggcgatgccg atccggtggc cctcgaccag cttccacatg gggttgtagg
      541 cggcgttcag gccggccttg acgcgccggt cctgcttggc gcagtggctg gtgcccgagc
      601 ggctggcacg ggcgcagaac ggcttggccg ggctggacag caggaagtcg atggcgtcct
      661 gggtccagtc gtagaaggtg ttgcccgtgg tctgggaggg gaagccctcg ttgcggtcgc
      721 cgccctcgcc gtaggtgtcc gacaggccct gcatctgggg gtcgaaggtg acgaccacgt
      781 acccggcctt ggccagcgtc tgggcggcgt accagtacag ctcctccgag gcctggatcg
      841 agccgttggt gatcacgatg gccgggcgct tgcgcgcgcc cgccctggtg gcccacacgc
      901 ggccggccac cgtggagccg ttgcgcgcag tccacagcac gcgctcgacc aggccgtatc
      961 cgttcgcctt ccagtcgttc aggcggatgt cgcccgcgca ggccagcccc ttgctgttgc
     1021 acaggtcggt caggaagttg cgctcggggt cccgggccgc ggtcagcgcg gcctcggtct
     1081 gggtgcgctg gctctccagg cccagctgca cctggtagcc gggctggagc cagacctgct
     1141 cgcgctcgcc gctcttggcc acgttggccg cctcgcgcac gggatcgaac gcgtgcgccg
     1201 acccggcacc cgccagcagc acggccaccg ccgccgcgat cgctcccgca acccgcatgg
     1261 gcgcgaagct accgcagcgg cggccacgcc gcgccggtcg ctcaggcggt ggtcaggact
     1321 ggctcggcca cgccggggcg cagccagttg acggccatgc ccaccccgat cgccaacacg
     1381 cccgcgccca tcgcgatggc gaacaccggc tccagcgcca tcggcccgaa catggtcagc
     1441 gcgagcacga tcggcatcca cagcggcagc acccgggccc ggtaggcggc cacgaacagc
     1501 agcacgccgc cgaccatcat cggcagcacc gcgctcatgg ccaggatcgc ggagccgggc
     1561 atgtcggcca cggcctcctc gaccttcacg gcctccttca gcgacagcgt ctcgtacagc
     1621 gcgatgccgt agaagtcgac cgccagcagg ccgggcaggc cgatggcgcc gaacacggcc
     1681 agcgcgccgc cggcggcgcg cagcctgccg ccgtgctcac ggcacagcca gtacacgccg
     1741 aagaacgccg gcaccagcgc catgtagccg aagtgcagca acagcgcggc gatctggccc
     1801 tgggtcgcgt tgctgccgat cgtggccagg tagtccgcgg tctcggtgct ggactcccag
     1861 ggggtggcca gcatgccggc cagcgtgatg acgccggcca cgagccagct gattgcggtg
     1921 aatctcggtg agagtgtcat ggcgcgatcg tgcgccgcgg cggcggctcg cgcaccgggg
     1981 aacaccccag tgacgtgcgt cgctcagttg ccctggatct cgcgcgcgtc gcccagcgcg
     2041 gcgtcgggca cgccgtaggc ctcgaccagc agcgccgcgt gctcggccag ctcgtcgcag
     2101 gcggtgttca cgcccttgag caccgccttg gaacgcgtgg aggacagctt gccgtgctcc
     2161 tggaaccagc cccggtcggc ctccacgcgg ctgagcgcgt acaggtcgca cagccgagcc
     2221 aggacggcct gcagcgccgg gtcgggacaa cgctcgacgg cgtcggcgaa ggcctccatg
     2281 accacgcgct cggcgtacac ccggcccacc agcaccacgt ggtcctggca gtcgttgagc
     2341 acctcgaacg ggtccgcgcc cgcgtccatg ccgcccttca ggcggcgcgc ggcggcggac
     2401 ttcacgtgct gctcgcgcca gcggaagaac tccagctggg tctcgcgctc gagcaggtcg
     2461 ccctcgtcgt cgcggccggg cagcaggtcg tcgccgatcc ggcccagcag ctcgcgcacg
     2521 gccgtgcgct cggccaccgt gcccgccacc tgctcggcca cgaacgcgaa ggtgccgaac
     2581 gggtccagct cgctgaactc gtgctggaag tcggtcagca ggttcttggc cgccagctgc
     2641 agcagcaccg tgttgtcgcc ctcgaacgtg gtgaacacgt cggtgtcggc cttgagcgcg
     2701 gcgaaccggt tggccttgag gtagccctgc ccgccacagg cctcgcggca gccctggatc
     2761 gtgtcggtcg cgtgccaggt gccgacggcc ttcagcgcgg ccgccatggt ctccagctcg
     2821 cggcgggtct cgtcgtcggc gccgtcctcg ccgaacacgt cctccagccc ggacaccagg
     2881 cgctcctggg cgaagtgcag cgcgtagttc tcggccagcg ggatcagcag ccgccggcgg
     2941 tgggtgcggt aggtcatgag cgtgacctcg tcgccgccgg gtggcccgaa ctggcgccgg
     3001 gcgttgccgt ggcgcacggc gatcgtcagc gccaccttcg aggcgttgat ggccgcgccg
     3061 cacacgctga tccggccctg gatgagcgtg ctgagcatgg tgaagaagcg cttggtgggc
     3121 ttctcgatcg ggctggtgta gccgccctcg ggcgacacgc tggcgtagcg gtccagcagg
     3181 ttctcgcgcg gcacgcgcac ctggtcgaac cacagccggc cgttgtccac gccgtccagg
     3241 cccagcttcg ggccgatgtc ctcgatccgc acgccctcgg cgggcttgcc cttctcgtcg
     3301 cggatgggca ccaccagcgc gtgcaccccg cgctcctggc cgccgaccac cagctggcag
     3361 aacacggccg ccaggacccc gtcgcaggcc gcgttgccga tccactcctt acgcgcccag
     3421 tcgtcgggcg tgtggatcac gaactcctcc gtgtccgggt cgtaggtcgc cgtcgtgcgc
     3481 agctcctgca cgttcg
//
LOCUS       DASUPW010000165         3161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_177194, whole genome shotgun sequence.
ACCESSION   DASUPW010000165 DASUPW010000000
VERSION     DASUPW010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177194"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VFY44_03790"
     CDS             <1..666
                     /locus_tag="VFY44_03790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEX5909588.1"
                     /translation="IMRRTKGRLEGRWAQPCFQRIDESKEGRPKIAAHRGLLGFPDGS
                     QNISPKTDDELIWTGREVPAWARGGTYLAARLIRLKIERWDRLSMTAQDDSIGRRKLS
                     GSPLEGGSEQSTPKLGRETPRDAHIRLANPRTPGSEKRRFLRRGFVYNDGFDEYGLLD
                     SGSVFLAFCRDVQEQFEATKRNMIGQDLDEYAVAIGGGYFYVPPGVDGRDDYLARRLV
                     EKS"
     gene            663..2954
                     /locus_tag="VFY44_03795"
     CDS             663..2954
                     /locus_tag="VFY44_03795"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX5909589.1"
                     /translation="MKQAGAFEVWAARAWNVFNEGKPFSIVYPVMVLLAATPLGLAPD
                     GGLGWALLGALAVAVPLSRFSFPLRGRALLWIAFAFSIPMLEPWRAPALLLGALAGWV
                     FFTVFFWGSLYYHLRTGAPWTNFTRFWRLVATNSDPTSGNMLEQVPKMVMALSAATLL
                     AEEPTGTSAGAIAVTVGVACAFGWMAWRRFDRDVLPAYPARFGRDAEPAASARRVYVI
                     VVDGANRERMWQAHTPTMDRLAREGTEYLDVDPAYPARTVVCFSSMLTGATPAEHGMR
                     SNFAPRLGVRAESVFQTLERAGKRGRLVGIAHLLDPFGEDVVRSVTSVQPTERIDHSL
                     TAAGRKVVEDEDPDLLVLQLLAADQLGHVRGTRNHEYLEQMEQTDRHVGDFLAFLGER
                     GKLDDATVILMADHGQGRGIGGHGHLDWGERPVPFVVWGKGAVPGAVSTEPRSVCELA
                     HTVSGLLGVDSPSAARGRALIPAGDPLVHAPPARDGRCLAIVVARDEEPRLPAVLDAL
                     PPEAAGMPVDVLVVDDGSQDATPDLARAAGAEVVAHPESLGLGAALRTGLEHARDAGY
                     AAAVYLDGDGEYDAAELARVRAPVARGRADYVLGSRFLGAREGMTWHRDLANRATTAL
                     LGTLMGTVLSDGQTGYRAFSAKALRAARINHDYNYAQVLTLSLWGAGIDPVEVPITYR
                     RRSGGRSFVSYPEYFARVTPALWRQFRASRTARASTAAPIPAASAYGQADSPKRGNSA
                     VSGPNGASGRGSTSPSEPSSTAR"
BASE COUNT          409 a         1190 c         1158 g          404 t
ORIGIN      
        1 atcatgcgcc gcacgaaggg ccgcctggag ggccgctggg cccagccctg cttccagcgc
       61 atcgacgagt cgaaggaggg ccggcccaag atcgccgccc accgcggcct gctgggcttc
      121 cccgacggct cgcagaacat ctcccccaag accgacgacg agctcatctg gaccgggcgc
      181 gaggtgccgg cctgggcccg aggcggcacc tacctggcgg cccgcctgat ccggctcaag
      241 atcgagcgct gggaccggct gtcgatgacc gcgcaggacg actcgatcgg ccgccgcaag
      301 ctgtcggggt cgccgctgga gggcggcagc gagcagtcga cgcccaagct gggccgcgag
      361 accccgcgcg acgcgcacat ccgcctggcc aacccgcgca ccccgggcag cgagaagcgc
      421 cgcttcctgc gccgcggctt cgtctacaac gacggcttcg acgagtacgg cctgctggac
      481 tccggctcgg tgttcctggc cttctgccgt gacgtgcagg agcagttcga ggccaccaag
      541 cgcaacatga tcggccagga cctggacgag tacgcggtgg ccatcggcgg cggctacttc
      601 tacgtgccgc ccggggtcga cggccgggac gactacctgg cccgccggct ggtcgagaaa
      661 tcttgaagca ggcaggcgcg ttcgaggtct gggccgcccg ggcctggaac gtcttcaacg
      721 agggcaagcc gttctcgatc gtctacccgg tgatggtgct gctggcggcc acgccgctgg
      781 ggctggcccc cgacggcggg ctgggctggg cgctgctggg cgccctggcc gtggccgtcc
      841 cgctgtcgcg gttctcgttc ccgctgcgcg gccgggcgct gctgtggatc gccttcgcct
      901 tctcgatccc catgctcgag ccctggcgcg cgccggccct gctgctgggc gcgctggccg
      961 gctgggtgtt cttcaccgtc ttcttctggg gctcgctgta ctaccacctg cgcaccgggg
     1021 cgccgtggac caacttcacc cgcttctggc gcctggtggc caccaactcg gaccccacca
     1081 gcggcaacat gctcgagcag gtgccgaaga tggtcatggc gctcagcgcg gccacgctgc
     1141 tggcggagga gccgaccggc accagcgccg gcgcgatcgc cgtcacggtg ggggtggcgt
     1201 gcgcgttcgg ctggatggcc tggcgccgct tcgaccgcga cgtgctgccc gcctacccgg
     1261 cccggttcgg ccgtgacgcc gagcccgccg ccagcgcacg gcgcgtctac gtgatcgtgg
     1321 tggacggcgc caaccgcgag cgcatgtggc aggcccacac gccgacgatg gatcgcctgg
     1381 cccgcgaggg caccgagtac ctggacgtgg accccgccta cccggcccgc accgtggtct
     1441 gcttctcgtc catgctcacc ggcgccacgc cggccgagca cggcatgcgc tccaacttcg
     1501 cgccccggct gggcgtgcgc gccgagtcgg tgttccagac gctggagcgc gcgggcaagc
     1561 gcggccggct ggtgggtatc gcacacctgc tggacccgtt cggcgaggac gtggtccggt
     1621 cggtgacatc ggtccagccc accgagcgca tcgaccactc gctgaccgcg gccgggcgca
     1681 aggtggtcga ggacgaggac cccgacctgc tggtgctcca gctgctggcg gcggaccagc
     1741 tgggccacgt gcgcggcacg cgcaaccacg agtacctgga gcagatggag cagaccgacc
     1801 gccacgtggg cgacttcctg gccttcctgg gcgagcgcgg caagctcgac gacgccaccg
     1861 tgatcctgat ggccgaccac ggccagggcc gcggcatcgg cggccacggc cacctggact
     1921 ggggcgagcg cccggtgccg ttcgtggtct ggggcaaggg cgccgtgccc ggcgccgtca
     1981 gcaccgagcc gcgctcggtc tgcgagctgg cccacaccgt cagcgggctg ctgggggtcg
     2041 actcaccgtc ggccgcccgc ggccgcgcgc tgatcccggc cggtgacccg ctggtgcacg
     2101 caccgccggc gcgcgacggg cgctgcctgg ccatcgtggt cgcccgcgac gaggagccgc
     2161 gcctgcctgc cgtgcttgac gcgttgcccc ccgaggccgc cgggatgccg gtcgacgtcc
     2221 tggtggtgga cgacggctcg caggacgcca cgccggacct ggcccgcgcg gccggggccg
     2281 aggtggtcgc gcacccggag tcgctgggcc tgggcgccgc gctgcgcacc ggcctcgagc
     2341 acgcgcgcga cgccggctac gcggcggcgg tgtacctgga cggcgacggc gagtacgacg
     2401 ccgccgagct ggcgcgggtg cgggcgccgg tggcccgcgg ccgcgccgac tacgtgctgg
     2461 gctcgcgctt cctgggcgcg cgcgagggca tgacctggca ccgcgacctg gccaaccgcg
     2521 ccaccaccgc gctgctgggc acgctgatgg gcaccgtgct cagcgacggc cagaccggct
     2581 accgcgcgtt ctccgcgaag gcgctgcgcg ccgcgcggat caaccacgac tacaactacg
     2641 cccaggtgct gaccctgtcg ctgtggggcg cgggcatcga cccggtggag gtgccgatca
     2701 cctaccggcg ccgctcgggc gggcgctcgt tcgtgtccta cccggagtac ttcgcccgcg
     2761 tgacgccggc gctctggcgc cagttccggg cctccaggac ggccagggcc agcaccgcgg
     2821 cgccgatccc cgcggccagc gcgtacggcc aggccgattc gccgaagcgc gggaacagcg
     2881 cggtcagcgg gccgaacggc gcgtcgggcc gtggctcgac cagcccgtcc gagcccagca
     2941 gcaccgccag gtagagccac accgacgaca ccgtcgtgag caccgccagg gcgatccccg
     3001 tgcgcggcag gcggcgcagc ccccaggcca cgagcggcac cgacagcgcc agcgcggcca
     3061 gcaggtgccg cggcgggaac cagtagccga acatggtcgg cgccaggaag ccggccacca
     3121 cgagctgcgc ccccagcgct gcggcgcaca tcgtggccgt g
//
LOCUS       DASUPW010000166         2760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_316659, whole genome shotgun sequence.
ACCESSION   DASUPW010000166 DASUPW010000000
VERSION     DASUPW010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316659"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VFY44_03800"
     CDS             <1..568
                     /locus_tag="VFY44_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5909590.1"
                     /translation="VFGAIVAVGDWIALAIIGVPAAGLWGLLAFVTNYVPAIGFVLGV
                     VPPAILGLLAGGWGELVAIVVVYSLLNFVIQTLIQPRFVGDSVGLSMTVTFLALLFWG
                     WVLGALGALLAIPLTLLVKALLVDVDPRGHWLDAILREEPRAPRTMRAKRRMHARRAA
                     LASVRRLHHPRRPTAPTTAEPDPAGHLQ"
     gene            677..940
                     /locus_tag="VFY44_03805"
     CDS             677..940
                     /locus_tag="VFY44_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909591.1"
                     /translation="MKGDRVEIVIDAGGSTRTYDIAATRAGRRVEVTHGRGVVEVAET
                     TRGGTPVRTARFMAGRVLALVEHPAPRPALDDDAAVTALRHSA"
     gene            1067..1486
                     /locus_tag="VFY44_03810"
     CDS             1067..1486
                     /locus_tag="VFY44_03810"
                     /inference="COORDINATES: protein motif:HMM:NF015927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HEX5909592.1"
                     /translation="MLESIGFAAAYTGVGIALLVLGFYALDLLTPGHLGRHIAEERSV
                     NAAVVLAAGFLGQGAIIFASIWTNATSGFGQALLYTVVFGILGVVLQAVAFVALDVLI
                     PGRLGHHLMEPEFHPASLVSAAVQLSVAAIIVASIWP"
     gene            1604..2371
                     /gene="fabG"
                     /locus_tag="VFY44_03815"
     CDS             1604..2371
                     /gene="fabG"
                     /locus_tag="VFY44_03815"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEX5909593.1"
                     /translation="MSEATAGTRVAIVTGAARGIGAATAIRLAQDGFAVAVLDLDEAS
                     AKGTVETIESAGGRALAVGADVSDSAQVEAAVERIAAELGGPTVLVNNAGVLRDNMLF
                     KMSDSDWDIVMNVHLRGAFLMTRAAQKHMIDAKWGRIVNLSSTSALGNRGQANYSTAK
                     AGLQGFTKTLAIELGKFGVTANAIAPGFIETEMTKATAERMGIPFEDFLKGAASQIPV
                     ARTGKPEDVAHLVSFFVSEGAGFVSGQVVYAAGGPKA"
     gene            2437..>2760
                     /locus_tag="VFY44_03820"
     CDS             2437..>2760
                     /locus_tag="VFY44_03820"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5909594.1"
                     /translation="MSDDVGQVERRGAVQVISINRPQARNALDAAVARLVAAAVERVA
                     AELGPPVVLINNAGITRDNMLFKMTDDDWDTVMNVHLRGAFLMSRAVQSQMVEQKFGR
                     IVNLSS"
BASE COUNT          365 a         1011 c          992 g          392 t
ORIGIN      
        1 ggtcttcggg gccatcgtcg cggtcggcga ctggatcgcg ctggcgatca tcggcgtccc
       61 cgcggcgggg ctgtgggggc tgctggcgtt cgtcaccaac tacgtgcccg cgatcggctt
      121 cgtcctgggc gtggtgccgc cggcgatcct gggcctgctg gccggtggct ggggcgagct
      181 cgtcgcgatc gtcgtcgtct actcgctgct caacttcgtg atccagacgc tgatccagcc
      241 gcgcttcgtc ggcgactccg tcggcctgtc gatgacggtc accttcctgg cgctgctctt
      301 ctgggggtgg gtgctcgggg cgctgggtgc cctcctcgcc atcccgctga cgctcctggt
      361 caaggccctc ctggtcgacg tcgacccgcg cgggcactgg ctggacgcca tcctgcgcga
      421 ggagccacgg gcgccgcgca ccatgcgcgc caagcgccgg atgcacgccc ggcgggccgc
      481 gctggcctcg gtccggcggc tgcaccatcc gcgccgtccg actgcgccga ccacggccga
      541 gccggaccct gcgggacacc tccagtaacg gcatccgcgc agctcagcac gcgtgtcgcc
      601 ggaatgtcgg acccctctgg gacgttgtcc ccacgaagcc ggtggcgtcg tgcgacgccc
      661 ggcggaggag gcaggcatga agggcgatcg ggtcgagatc gtgatcgacg cgggcggcag
      721 cacgcgcacc tacgacatcg cggcgacgcg ggccggccgg cgggtcgagg tgacccacgg
      781 ccggggcgtg gtggaggtgg ccgagaccac ccgcggaggg acgccggtgc gcacggcgcg
      841 gttcatggcc gggcgggtgc tcgcgctcgt ggagcacccc gcgccacggc cggcgctcga
      901 cgacgatgcg gcggtgaccg cgctgcggca ctccgcctga gagacgctgt gacgcacccg
      961 ccgggcgtgt gaggcccccg gtccgtgcgg agaggtgacc ggtgcggtag acctggcagg
     1021 ccgtcggccc cgtcgccgcg cccgtcacca cctggaggtc caccccgtgc tggagagcat
     1081 cggcttcgcc gccgcctaca caggggtcgg catcgcgctg ctggtcctgg gcttctacgc
     1141 cctcgacctg ctcacccccg ggcacctggg ccggcacatc gccgaggagc ggtcggtcaa
     1201 cgccgccgtc gtcctcgccg cgggcttcct ggggcagggc gcgatcatct tcgccagcat
     1261 ctggaccaac gcgaccagcg ggttcggcca ggcgctgctc tacaccgtgg tgttcggcat
     1321 cctcggcgtc gtgctgcagg ccgtggcgtt cgtcgccctc gacgtgctca tccccgggcg
     1381 gctcggccac cacctcatgg agccggagtt ccacccggcc agcctggtca gcgcggccgt
     1441 ccagctgtcg gtcgccgcga tcatcgtcgc ctccatctgg ccctgacggt cgtcgcccgg
     1501 accttgtgac gggcgccgca ggacgcggac gtaacgtgtc gagcgcagct gcggccgcgc
     1561 cgctcgtacc ggcgccggcc ccacccacgt gagaggaaca ttgatgagcg aggcaaccgc
     1621 aggcacccgt gtggccatcg tgaccggggc cgcccgcggc atcggcgcgg caaccgcgat
     1681 ccggctggcc caggacgggt tcgccgtcgc cgtgctggac ctggacgagg cctccgcgaa
     1741 gggcacggtc gagaccatcg agtccgccgg tggccgggcg ctggccgtgg gcgccgacgt
     1801 gagcgactcg gcgcaggtcg aggccgccgt cgagcggatc gccgcggagc tcggcggccc
     1861 gaccgtgctg gtcaacaacg ccggcgtgct gcgggacaac atgctgttca agatgtccga
     1921 cagcgactgg gacatcgtca tgaacgtgca cctgcggggc gccttcctca tgacccgcgc
     1981 ggcgcagaag cacatgatcg acgccaagtg gggccggatc gtcaacctgt ccagcacctc
     2041 ggcgctgggc aaccgcggcc aggccaacta ctcgacggcc aaggccggcc tgcagggctt
     2101 caccaagacc ctggccatcg agctgggcaa gttcggcgtc accgccaacg ccatcgcccc
     2161 gggcttcatc gagaccgaga tgaccaaggc gaccgccgag cgcatgggca tcccgttcga
     2221 ggacttcctc aagggcgccg cctcgcagat cccggtggcc cgcaccggca agcccgagga
     2281 cgtcgcgcac ctggtgtcct tcttcgtcag cgagggtgcc ggcttcgtgt ccggccaggt
     2341 cgtctacgcc gccggcggtc cgaaggcctg atccaccgcg gggcccggcc gccgtgcggc
     2401 gccgggcccc gcgtccgctc caccggaggc gggcacgtgt ccgacgacgt cggccaggtc
     2461 gagcgccgcg gcgccgtcca ggtcatcagc atcaaccggc cgcaggcgcg caacgcgctc
     2521 gacgccgcgg tcgcccgcct ggtggccgcc gccgtcgagc gggtggccgc cgagctcggg
     2581 ccgcccgtcg tcctgatcaa caacgccggg atcacccggg acaacatgct gttcaagatg
     2641 accgacgacg actgggacac ggtcatgaac gtgcacctgc gcggcgcgtt cctcatgagc
     2701 cgcgcggtgc agagccagat ggtcgagcag aagttcggcc ggatcgtgaa cctgtcgagc
//
LOCUS       DASUPW010000167         4771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_593256, whole genome shotgun sequence.
ACCESSION   DASUPW010000167 DASUPW010000000
VERSION     DASUPW010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593256"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /gene="dtd"
                     /locus_tag="VFY44_03825"
     CDS             complement(<1..278)
                     /gene="dtd"
                     /locus_tag="VFY44_03825"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR00256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX5909595.1"
                     /translation="MRAIVQRVSQASVSVDGEEVSAIGPGLLVLLGVHRDDSEEQADR
                     MAGKLGALRIFEDEEGRMNLSVADTGGQILCVSQFTLYGDTRKGNRPG"
     gene            302..730
                     /locus_tag="VFY44_03830"
     CDS             302..730
                     /locus_tag="VFY44_03830"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEX5909596.1"
                     /translation="MAAVDVIDQYLEALPGDSRRLAHGEWGVTVAPEQAGGWPLHVGL
                     VLQDGLVRAQAHALDSPDGIDPWMLMWWNRQTRMARFGCTREREVWVHADVPVAAVDE
                     REIDRVLGLVVEGAVAVREYQRSRLEPAPEAGGGWLDGAS"
     gene            complement(788..1126)
                     /locus_tag="VFY44_03835"
     CDS             complement(788..1126)
                     /locus_tag="VFY44_03835"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5909597.1"
                     /translation="MYTRGSALLDAGDFAAATVPLEQARRLEPGKTSIREALGRAYFR
                     SGRFEEAREEFAAVVEQAPVNDYAHFCLGKALANTGRRAAARRHAVLAASMRPDRADY
                     RALRDRLKTA"
     gene            complement(1180..2430)
                     /gene="coaBC"
                     /locus_tag="VFY44_03840"
     CDS             complement(1180..2430)
                     /gene="coaBC"
                     /locus_tag="VFY44_03840"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX5909598.1"
                     /translation="MARILLGVSGGIAAYKAVELTRLAMAAGHSVRVVQTESSLRFVG
                     RATFEGITGAPVLVDEFEPDPARGAFPGDPEPDHAAISHLELVRRADAFVIAPASANT
                     LAKLAGGHADNLLSSAALACTAPLVLAPAMNNHMWEHPATGANVETLRLRGAAIVEPE
                     TGPLASKGEWGAGRLADPATILARVESVLAGGSGALSGLRVLVTAGGTREPIDSVRYV
                     GNRSSGRMGVALAAEAAARGAEVTLVGANLAVAAPAGVRAVTVQTAAQLRDAVEAEFD
                     ATDVLLMAAAVADFRPAGPAADKIKKTGREGLTVELEPTDDVLAGVSARRSPGQTLVG
                     FAAEHGEGGLEHARDKLARKGLDAVVLNDIARRDIGFDTDQNEVTVITGSGERAVPIG
                     SKRAVAAVILDEVERMRTAHGARS"
     gene            complement(2481..2711)
                     /gene="rpoZ"
                     /locus_tag="VFY44_03845"
     CDS             complement(2481..2711)
                     /gene="rpoZ"
                     /locus_tag="VFY44_03845"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX5909599.1"
                     /translation="MIKPRLDTLLEQVDSHYACVLVAAKRARQINSYYHNLGEGTFDE
                     YPPPMVETGSKNYLKIALDEVAEGKIKYRYRD"
     gene            complement(2708..3259)
                     /gene="gmk"
                     /locus_tag="VFY44_03850"
     CDS             complement(2708..3259)
                     /gene="gmk"
                     /locus_tag="VFY44_03850"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX5909600.1"
                     /translation="MITGPSGVGKGTLIKLLLERSPGLQLSVSATTRQPREGEVNGRE
                     YHFLSREDFDQRVENGEFIEHAEYAGNRYGTLRSELERPADGIVLEIEIQGARQVRET
                     LPEAYQVFIAPPSLEALRERLEGRASDTDEQIARRLEVAPVEMAAQEEFRKVVVNADL
                     DEALEELVALAATMSAPDEPESP"
     gene            complement(3289..3642)
                     /gene="mihF"
                     /locus_tag="VFY44_03855"
     CDS             complement(3289..3642)
                     /gene="mihF"
                     /locus_tag="VFY44_03855"
                     /inference="COORDINATES: protein motif:HMM:NF041260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HEX5909601.1"
                     /translation="MPVANETSSQSSSAAGLTPERSLTQRMDALQRANEIRTRRATLK
                     RDLKAGRVKIHGLLLDPPKYLETAKVFDLLLAVPKYGRVKVNRILTHCRISPSKTIGG
                     LSERQRNELVSYLRR"
     gene            complement(3730..4632)
                     /locus_tag="VFY44_03860"
     CDS             complement(3730..4632)
                     /locus_tag="VFY44_03860"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEX5909602.1"
                     /translation="MSDLAIDLCGVALDGPVLNASGTFDAIAARRAFGDALLERFPFD
                     AFVTKTVTLAPRQGNPPPRLWETPGGMVNSIGLPNKGLEGFLAQDLPQLAELPVPLVV
                     SVMGFDHQELARLVETVAARDEVAMLELNVSCPNVETGVLVGADPGETDAAVRAVRDR
                     TAKPLVVKLSPNSVDPSAVARAAAGAGADAVSLINTLKGMPLHPRTREPWLGGGTGGL
                     SGPAVRVVALEQVAAVSRAVDVPIVGMGGITSGGDALDFLTAGATVIAVGTESFRDPS
                     VGRRIGAELSALGGRETIRKHQPV"
BASE COUNT          633 a         1733 c         1709 g          696 t
ORIGIN      
        1 aaccccggcc ggttgccctt gcgcgtgtcc ccgtaaaggg tgaactggct cacgcacagg
       61 atctggccgc cggtgtcggc caccgacagg ttcatccgcc cctcctcgtc ctcgaagatc
      121 cgcagcgcgc cgagcttgcc ggccatcctg tcggcctgct cctcggagtc gtcgcggtgc
      181 accccgagca gcaccagcag gccgggcccg atcgcggaca cctcctcgcc gtcgacgctc
      241 accgacgcct gcgagacccg ctggacgatc gccctcacgc cgggcaccgt actgtcaggg
      301 catggccgca gtggacgtca tagaccagta cctggaagcg ctgcccggcg actcccgccg
      361 cctggcgcac ggcgagtggg gcgtgaccgt cgcccccgag caggccggcg gctggccgct
      421 gcacgtgggc ctggtgctcc aggacggcct ggtgcgcgcc caggcgcacg cgctcgactc
      481 gcccgacggc atcgacccct ggatgctgat gtggtggaac cgccagaccc ggatggcccg
      541 cttcggctgc acccgcgagc gcgaggtgtg ggtgcacgcc gacgtcccgg tggccgccgt
      601 cgacgaacgc gagatcgacc gcgtcctggg cctggtcgtg gagggcgcgg tggcggtgcg
      661 cgagtaccag cgctcgcgcc tggagccggc gcccgaggcg ggcggcggct ggctggacgg
      721 cgcctcctag gcagcgcgcg ttcggtttct cccgccaggc gggaggaatg cgccgcgcgc
      781 ggtgcggcta ggccgtcttg agccggtcgc gcagcgcccg gtagtcggcg cggtccgggc
      841 gcatcgacgc ggccaggacg gcgtggcggc gggcggcggc gcggcggccg gtgttggcca
      901 gcgccttgcc caggcagaag tgggcgtagt cgttgaccgg ggcctgctcg accacggcgg
      961 cgaactcctc gcgcgcctcc tcgaaccgcc ccgagcggaa gtaggcccgg cccagggcct
     1021 cgcggatcga ggtcttgccg ggctcgagcc tgcgggcctg ctccagcggg accgtcgccg
     1081 cggcgaagtc accggcgtcc agcagcgccg agccgcgcgt gtacaggtcg tagacccgct
     1141 cgccgcggtc gcgctcctcg cgctcgcgtt cctactcgct cacgagcgag ctccgtgtgc
     1201 ggtcctcatc ctttccacct catcgaggat gacagccgcc acggcacgct tggagccgat
     1261 cgggacggcg cgctccccgg atccggtgat cacggtgacc tcgttctggt ccgtgtcgaa
     1321 gccgatgtcg cggcgggcga tgtcgttgag caccacggcg tccaggccct tgcgggccag
     1381 cttgtcgcgc gcgtgctcca ggcccccctc cccgtgctcg gcggcgaaac cgaccagcgt
     1441 ctggcccggg ctccggcgcg cggacacgcc ggccagcacg tcgtcggtgg gctccagctc
     1501 cacggtcagc ccctcgcggc ccgtcttctt gatcttgtcc gcggccgggc cggcgggccg
     1561 gaagtcggcc accgccgccg ccatcagcag cacgtcggtg gcgtcgaact cggcctcgac
     1621 cgcgtcccgg agctgggcgg cggtctgcac ggtcaccgcg cggacgccag cgggggcggc
     1681 gacggccagg ttggcgccca ccagcgtgac ctcggcgccg cgggcggcgg cctcggcggc
     1741 cagcgccacc cccattcgcc cggacgagcg gttgcccacg taacggacgg agtcgatcgg
     1801 ctcgcgggtc ccgccggcgg tcaccaggac gcgtaggccc gagagcgcgc ccgagccgcc
     1861 ggccagcacc gactcgaccc gggccaggat cgtggccggg tcggccagcc gtccggcgcc
     1921 ccactcgccc ttggaggcca gcgggccggt ctcgggctcg acgatggcgg cgccgcgcag
     1981 gcgcagggtc tcgacgttcg cgcccgtggc cgggtgctcc cacatgtggt tgttcatcgc
     2041 cggggccagc acgagcgggg cggtgcaggc cagggcagcg ctgctgagca ggttgtcggc
     2101 gtggcctccg gccagcttcg ccagcgtgtt ggccgaggcc ggcgcgatca cgaacgcgtc
     2161 ggcgcgccgg accagctcca ggtgcgagat ggccgcgtga tcgggctcgg ggtcgcccgg
     2221 gaaggcgccc cgggcggggt cgggctcgaa ctcgtcgacc agcacgggcg cgccggtgat
     2281 gccctcgaag gtggcccggc ccacgaagcg caggctggac tcggtctgca ccacgcggac
     2341 ggagtgcccg gcggccatgg ccagccgggt cagctcgacg gccttgtagg cggcgatgcc
     2401 gccggagacg cctaggagga tgcgggccat caggccctca acggtacccc agccccggcg
     2461 ggctcaggcg ccgtgaatcc ctagtcccgg tagcggtact tgatcttgcc ctcggcgact
     2521 tcgtccaggg cgatcttgag gtagttcttc gagccggtct cgaccatcgg cggcgggtac
     2581 tcgtcgaacg tcccctcgcc gaggttgtgg tagtacgagt tgatctgccg ggcgcgcttg
     2641 gcggccacga gcacgcaggc gtagtgagag tcgacctgct cgagaagtgt gtccaggcgg
     2701 ggcttgatca aggggattcc ggttcgtcgg gggcagacat cgtagcggcg agcgcgacga
     2761 gctcctccag cgcctcgtcc aggtcggcgt tcacgacgac cttccggaac tcctcctggg
     2821 ccgccatctc gaccggtgcg acctccagcc ggcgggcgat ctgctcgtcg gtgtccgagg
     2881 cgcggccctc cagccgctcc ctgagcgcct ccagcgaggg cggtgcgatg aacacctggt
     2941 aggcctcggg aagggtctcg cggacctggc gcgcgccctg gatctcgatc tcgagcacga
     3001 tcccgtcggc cgggcgctcg agctccgagc gcagcgtgcc gtaacggttc ccggcgtact
     3061 cggcgtgctc gatgaactcg ccgttctcga ccctctgatc gaagtcctca cgactcagaa
     3121 agtgatactc gcggccgttc acctcgccct cgcgcggctg gcgggtggtg gcggacaccg
     3181 acagctggag tcccgggctg cgttcgagca gcagcttgat gagcgttccc ttgccgacgc
     3241 cggagggacc cgtgatcacc aggaccgggg tctgggggga tcgtgcctct atcgccggag
     3301 gtacgagacg agctcgttgc gctggcgctc ggacaggccg ccgatggtct tgctcggcga
     3361 gatccggcag tgcgtgagaa tccggttcac cttgacccgg ccgtacttgg gcaccgccag
     3421 cagcaggtcg aaaaccttgg ccgtttccag gtatttcggc ggatcgagca gcaggccgtg
     3481 gatcttcacg cgccccgcct tgaggtcgcg cttgagcgtg gcccggcgcg tccggatctc
     3541 gttcgcgcgc tggagggcgt ccatgcgctg ggtcagcgag cgctcggggg tgaggccggc
     3601 ggcggaggag ctctgggagg aagtttcgtt cgcgaccggc atgaggcggc gatcatacac
     3661 acggatttcg caacgtcgac gcctctcgac gcttttcctg cctaattcat ctgaacctct
     3721 agttgaggtt cagactggtt gatgtttcct gatggtttcg cgacctccca aggcggacag
     3781 ctctgccccg atccggcgtc ccacggacgg gtcacgaaag ctctccgtgc ccactgcgat
     3841 caccgttgcg cccgccgtca ggaagtccag cgcgtcgcca cccgaggtga taccgcccat
     3901 ccccacaatg ggtacatcaa ccgccctgga cacggccgcg acctgctcca gggccaccac
     3961 ccggaccgcc gggccggaca gcccgccggt gccgccaccg agccagggct cgcgcgtccg
     4021 ggggtgcagc ggcatcccct tgagcgtgtt gatcagcgaa acggcgtcgg ccccggcgcc
     4081 ggccgccgcc cgcgcgaccg cggagggatc gaccgagttg gggctgagct tgaccaccag
     4141 cggcttggcg gtccggtcgc gcaccgcccg gacggcggcg tcggtctccc ccgggtcggc
     4201 gcccaccagc acgccggtct ccacgttggg gcaggacacg ttcagctcga gcatcgccac
     4261 ctcgtcgcgg gcggccaccg tctccacgag ccgtgccagc tcctggtggt cgaagcccat
     4321 caccgacacc accagcggca ccgggagctc cgccagctgg ggcaggtcct gggcgaggaa
     4381 gccctccagc cccttgttgg gcagcccgat ggagttgacc atccccccgg gggtctccca
     4441 cagccggggc ggcgggttgc cttggcgcgg cgccagggtc acggtcttgg tcacgaaggc
     4501 gtcgaacggg aagcgctcca gcagcgcgtc cccgaaggcg cgcctcgcgg caatggcgtc
     4561 gaaggtgccc gaggcgttca gcaccggccc gtccagggcc acgccgcaca ggtcgatcgc
     4621 caggtcgctc atgccaccgc cagctcggcg ccggtgaaca cgggcccgtc gacgcacacc
     4681 cgctgatagc ccgcggccgt gggcaccacg cacccgaagc aggcgccgta gccgcacgcc
     4741 atccccgact ccagcgccag ctggcagtcg a
//
LOCUS       DASUPW010000168         3923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_177974, whole genome shotgun sequence.
ACCESSION   DASUPW010000168 DASUPW010000000
VERSION     DASUPW010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177974"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="VFY44_03865"
     CDS             complement(<1..1268)
                     /locus_tag="VFY44_03865"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HEX5909603.1"
                     /translation="MEILQEIGSYAGLAAVLGLAVLSALYFSQARDVKRLREWAGRAP
                     ERAPETTGVQRVAAKPVPRAPGQAAATQAGAPAAPGAKPVPPAPAGARAAAAGAAPAT
                     AVGPAAATPAARAEAAKTGKDDEEADATGSDNGTDEGNVLSQDTVVHPPPPPPGEDDD
                     EYDEDFDDTGDHEQVSEEDDALDDDGDWDDTGEHDPTDPESHWEDTGDEPAIAPRPAT
                     PAASRPIAPGAPVPAAGTPTPSASASTSPATSSGGSILPPYAHSRPGGRAPEGGNGGP
                     RLFASRGRAIAVVAAGVLVLGGIALGATQLLGGDDEPAGSAGGDNASQNAPATETTPS
                     KGGGKKPPLNRGNVRVAVLNGTTVPGLAAQIGDQIERQGFRLGTVTNFNDQQRAESVV
                     LYAPGAEREAAEVGRRLKISQREPIDAESQG"
     gene            complement(1294..3213)
                     /locus_tag="VFY44_03870"
     CDS             complement(1294..3213)
                     /locus_tag="VFY44_03870"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEX5909604.1"
                     /translation="MRRLGQVVVDACLVALAYYLAFVLRFDSGIPERYQELLAVTIGF
                     VVVLKVALFAAFGLYSKLWRFVDQADFESILKAVVVSSFVLIAGLFLLPFGVSDPPRG
                     VMALDFLITLALVGGARFLVRAVVERPMRQPLVQTADTEVLVVGAGNGGQQVALELRR
                     NPELSSAVLGFVDDDPRKRGMLVAGHRVFGTTDDLPRILDDTKPDEVVIAIPSAPGEL
                     RLKVVTACRDRDIPVRTLPTVFELLSRGSNLLRQVREVKVEDVLGREPVRSEIETVGS
                     YLRDRVVVVTGAGGSIGAELCRQIARVKPRRLILVDHAENNLFEIRRHLEDEQHFGSL
                     AAVLADCKDATRMQELFADEKPSVVFHAAAYKHVGLMEENPVEAVRNNAVATRIVAAA
                     AGEAGVERFVLVSTDKAVAPATVMGASKALAEWAVEAAQNRYRDTRYASVRFGNVLGS
                     SGSVVPIFRRQIEAGGPVTVTDAEMTRYFMTIPEAVQLIIHAADLVPTPSGPRAGESN
                     AQPRSSTFVLEMGDPVKIVDLARNMIRLSGKEPDVDIAIEVVGRRPGEKIHEELFEAG
                     ERPEPTPADRIVAAVRPRLDASWVEDAFARIEDLVFNGDAAALADAVAQMSAERSLTA
                     HYAPEPGDRGVPDPR"
     gene            complement(3240..>3923)
                     /locus_tag="VFY44_03875"
     CDS             complement(3240..>3923)
                     /locus_tag="VFY44_03875"
                     /inference="COORDINATES: protein motif:HMM:NF015940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HEX5909605.1"
                     /translation="PAATAFDYEWAKVQHNVNCRLARAIVVPDAIPPERLDRYGAKGK
                     VRAYPGLKEEYYLADFAADRAVLDELGLDDSQPIAVVRTPPAVSLYHRFENPVFARVL
                     DRLAATAQTVVLPRTAEQRAELDGRDGLIVPERAIDAQSLIAFSDLVVSAGGTMNREA
                     VALGAPVFTTFEGKLGAVDERLLDEGRLRRLEDPDQVQVTKRAKHDGDRIRRDPADLA
                     KLLLSAREQ"
BASE COUNT          491 a         1408 c         1481 g          543 t
ORIGIN      
        1 ccctggctct cggcgtcgat gggctcgcgc tgggagatct tcagccgccg gcccacctcg
       61 gcggcctcgc gctcggcgcc gggggcgtag agcaccaccg actcggcgcg ctgctggtcg
      121 ttgaagttgg tgaccgtgcc caggcggaag ccctggcgct cgatctggtc gccgatctgc
      181 gcggcgaggc cggggaccgt cgtgccgttg agcacggcga cgcgcacgtt gccgcggttg
      241 agcggcggct tcttgccccc gcccttgctg ggcgtggtct cggtcgcggg cgcgttctgg
      301 gaggcgttgt caccgccggc ggaaccggcc ggctcgtcgt cgccgcccag gagctgggtg
      361 gcgcccagcg cgatgccgcc gagcaccagg acgccggccg cgaccacggc gatggcgcgg
      421 ccgcgcgagg cgaacagccg tgggccgccg ttgccgccct cgggggcgcg gccgccggga
      481 cggctgtgcg cgtacggggg caggatgctg cccccgctcg aagtcgcggg cgacgtggag
      541 gcggaggcgg agggggtcgg cgtgcccgcg gcgggcacag gcgcgccggg cgcgatcggg
      601 cgcgaggcgg ccggcgtggc gggacgcggc gcgatcgcgg gctcgtcgcc ggtgtcctcc
      661 cagtgcgact cggggtcggt ggggtcgtgc tcgcccgtgt cgtcccagtc gccgtcgtcg
      721 tcgagggcgt cgtcctcctc ggagacctgc tcgtggtcgc cggtgtcgtc gaagtcctcg
      781 tcgtactcgt cgtcgtcctc ccccgggggc gggggcggcg ggtgcacgac agtgtcctgg
      841 gacaggacgt tgccctcgtc ggtcccgttg tcggagccgg tggcgtcggc ctcctcgtcg
      901 tccttcccgg tcttggccgc ctcggcgcgc gcggccggag tggcggcggc agggccgacc
      961 gcggtggcgg gcgcggcccc ggcggccgcg gcgcgggctc cggccggcgc cggcgggacg
     1021 ggcttggccc ccggtgcggc gggcgccccg gcctgcgtgg cggccgcctg gcccggtgcg
     1081 cgcggcaccg gcttggcggc cacgcgctgc acgcccgtgg tctcgggtgc gcgctcgggg
     1141 gctcgccccg cccactcccg cagccgcttg acgtcgcggg cctgcgagaa gtacagggcg
     1201 gagagcacgg ccaggcccag gacggcagcc aggccggcgt acgagccgat ctcctgaaga
     1261 atctccatcg agaaggtgtg aagggtaacc gcgctaccgg gggtccggca cgccgcggtc
     1321 ccctggctcg ggtgcgtagt gcgcggtcag ggaccgctcg gccgacatct gggccaccgc
     1381 atcggccagc gcggcggcgt ccccgttgaa gaccaggtcc tcgatccggg cgaaggcgtc
     1441 ctcgacccag gaggcgtcca gccgggggcg gacggcggcc acgatccggt cggccggcgt
     1501 gggctcgggc cgctcgccgg cctcgaacag ctcctcgtgg atcttctcgc ccggcctccg
     1561 gccgacgacc tcgatggcga tgtccacgtc cggctccttg cccgacaggc ggatcatgtt
     1621 gcgggccagg tccacgatct tgaccgggtc gcccatctcc aggacgaagg tggacgagcg
     1681 cggctgggcg ttggactcgc cggcacgcgg gcccgagggc gtgggcacca ggtcggcggc
     1741 gtggatgatc agctgcacgg cctcggggat ggtcatgaag tagcgcgtca tctccgcgtc
     1801 ggtcaccgtg accggcccgc ccgcctcgat ctgacgccgg aagatgggca ccaccgagcc
     1861 cgaggagccc agcacgttgc cgaaccgcac cgacgcgtag cgcgtgtcgc ggtagcggtt
     1921 ctgggcggcc tcgacggccc actcggccag ggccttggac gcccccatga cggtcgccgg
     1981 cgccacggcc ttgtccgtgg acaccaggac gaaccgctcc acgcccgcct cgcccgcggc
     2041 ggcggccacg atgcgcgtgg ccaccgcgtt gttgcgcacg gcctcgacgg ggttctcctc
     2101 catcaggccc acgtgcttgt aggcggcggc gtggaagacc acggacggct tctcgtcggc
     2161 gaacagctcc tgcatccgcg tggcgtcctt gcagtcggcc agcaccgcgg ccagcgagcc
     2221 gaagtgctgc tcgtcctcca ggtggcggcg gatctcgaag aggttgttct cggcgtggtc
     2281 gaccaggatc agccggcgcg gcttgacgcg cgcgatctgg cggcacagct cggcgccgat
     2341 cgatccgccc gcgccggtga ccacgaccac ccggtcgcgc aggtaggagc ccaccgtctc
     2401 gatctcggag cgcacgggct cgcgccccag cacgtcctcg accttgacct cgcgcacctg
     2461 gcgcagcagg ttggagccgc gcgagagcag ctcgaacacc gtgggcagcg tgcgcacggg
     2521 gatgtcgcga tcgcggcagg cggtcacgac cttgaggcgc agctcgcccg gggccgacgg
     2581 gatggcgatg accacctcgt cgggcttggt gtcgtcgagg atgcggggca ggtcgtcggt
     2641 ggtgccgaac acgcggtgcc cagccaccag catgcccctc ttgcgcgggt cgtcgtccac
     2701 gaagcccagg acggcgctgg acagctcggg gttgcggcgc agctccaggg ccacctgctg
     2761 gccgccgttg ccggcgccca cgaccaggac ctcggtgtcg gcggtctgca cgagcggctg
     2821 gcgcatcggc cgctcgacca cggcgcggac caggaaccgg gccccgccca ccagcgccag
     2881 cgtgatcagg aagtccagcg ccatgacccc gcgcggcggg tccgagacgc cgaagggcag
     2941 caggaacagg ccggcgatga gcacgaagct cgagaccacg acggccttga ggatcgactc
     3001 gaagtccgcc tggtcgacga accgccacag cttggagtag aggccgaacg cggcgaacag
     3061 cgccaccttc aggaccacga cgaacccgat cgtgaccgcc agcagctcct ggtagcgctc
     3121 ggggatgccc gagtcgaagc gcaggacgaa cgccaggtag tacgcgagcg cgaccaggca
     3181 ggcgtcgacc accacctgtc cgaggcggcg cacggcgacc gaggatgtca tccgtttgct
     3241 cactgttctc gcgcggatag cagcagtttg gccaaatcgg ccgggtcccg gcggatccgg
     3301 tccccatcgt gcttcgcccg cttggtcacc tggacctggt cgggatcctc gaggcggcgt
     3361 aggcgccctt cgtcgagcag ccgctcgtcc accgcgccca gctttccttc gaacgtggtg
     3421 aacaccgggg cccccagtgc caccgcctcg cggttcatgg taccgccggc ggagacgacg
     3481 aggtcgctga atgcgatcag gctctgcgcg tcgatcgcgc gctcgggcac gatcaggccg
     3541 tcgcggccgt ccagctcggc gcgctgctcg gccgtgcgcg gcagcacgac ggtctgggcg
     3601 gtggccgcca gccggtccag gacccgcgcg aacaccgggt tctcgaaccg gtggtagagc
     3661 gacaccgcgg gcggtgtgcg gacgacggcg atcggctgcg agtcgtccag ccccagctcg
     3721 tccagcacgg cccggtcggc cgcgaagtcg gccaggtagt actcctcctt caggcccggg
     3781 tacgcgcgca ccttgccctt ggcgccgtag cggtccaggc gctcgggcgg gatcgcgtcc
     3841 ggcaccacga tcgcccgcgc gaggcggcag ttgacgttgt gctggacctt cgcccactcg
     3901 tagtcgaacg ccgtggcggc cgg
//
LOCUS       DASUPW010000169         3629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1148604, whole genome shotgun sequence.
ACCESSION   DASUPW010000169 DASUPW010000000
VERSION     DASUPW010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148604"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(326..2368)
                     /locus_tag="VFY44_03880"
     CDS             complement(326..2368)
                     /locus_tag="VFY44_03880"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX5909606.1"
                     /translation="MPTTTATLSAPLGRLLFTVRLLVQGNPALGPGLLAMVAFVAMAG
                     SEAGFYPSLWYGIALLTLALLVATVVAVGIPRDVPRTVLAGVLLMAGFAAWQYLSIAW
                     AGENGQALDGANRTLFYTTVLALFAFWPWNPRGARLVLGTFGLGISAIALVQLLKFQS
                     DPLPYLNDARFFEPAGYINANVAMWVIATFPCLYLGAAREVAPLSRGLFLACAGLLSG
                     VTLMGQSRGWLVAIPFAVLIFVVTSRHWRRSLVAVLAVAAGTAAISSSLLAVHDEFSA
                     GAIDDMASEAALGILVVAALLGLGGTLAGMAERRLKPAGQEFRRRRERAVGAALIVIV
                     LALGAGAWAKAGNPIERLDNSWQQFKSLDTQAGQGQSRFSTGGTNRYDFWVVAWDLFG
                     EHPVRGVGADGFQPYYLRQGKSLERPRYAHSLQLGVLAQTGLVGALLAFGAMAALAAA
                     ALRAIAKGYAPVGAAAGAAAAAFAYWFLHGSVDWFWEFAGLGAPALALLGLAAGLAPR
                     GNSPVPRPPLPTSSLPTGVLAAGGVAVAVATVFLAGPWIADLEIDRAAKAWRGSPDGA
                     FKRLDRARKLNPMSAQADLSAGTIALRLGRLDQAERSFRRARAREPENAYALLELGVI
                     AAERDDRAEATALLRRVTRLNPRDEIAARALRDVRAGRYVTTARINRLLLQRARGE"
     gene            2358..2504
                     /locus_tag="VFY44_03885"
     CDS             2358..2504
                     /locus_tag="VFY44_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909607.1"
                     /translation="MVGKIVYWLAVVAISLALVIALILFFESRDDSSLEGAAVPAPVA
                     LTGA"
     gene            complement(2491..3099)
                     /locus_tag="VFY44_03890"
     CDS             complement(2491..3099)
                     /locus_tag="VFY44_03890"
                     /EC_number="3.4.22.70"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEX5909608.1"
                     /translation="MKAVLRFVASVLMVSGVLMIADAGATLLWQEPVSAFVAQRQQND
                     LEEAFAATPESQPKPKEGRPPKQKPIPGSAVAQLSFPTLDRKYYVIEGTKTTDLRKGP
                     GHYPDTALPGQSKTVAIAGHRTTYGAPFRTIDKLKRGDQIDLRMRYGTYVYKVQKTRI
                     VAPTETSVTDNVGYDRLVLTACHPLYSAAQRIVVFARLVKRP"
     gene            complement(3109..>3629)
                     /locus_tag="VFY44_03895"
     CDS             complement(3109..>3629)
                     /locus_tag="VFY44_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909609.1"
                     /translation="HYEGILKIDPATAEAVEPIDEVQEAVVQCMDCMHDQPHLSLTFY
                     EEDGRRVSRIEAVEDRWERMVTGTPWVASCTVAVDESEVESCSGPEAADSLAFAAFGD
                     HGTREFFTHVRFHKHDDSGQIAIHMLIELYARSSDEASEVLTSAARGTLELTSLAEES
                     RPPAAGGRDDNH"
BASE COUNT          516 a         1267 c         1332 g          514 t
ORIGIN      
        1 ttgccctcga tctgattctg gacggcgccg tcgtcgggcg tgtagccgtc cacggaggac
       61 tgcgcgaacg cgaccgatgt cagcgacatc gccatcaaaa ctgaggcgag tgcgattgca
      121 agtgtctttc tcatctgttg cgtagtccct tggattcgag ttccggtcga aaagcgtcgt
      181 agttcgccga ccccagccta gcccttcctt gggccgctga accctagcgg gcagggccga
      241 cacccccgta ccgcagaagt cttttgtagc acggtagttg gcatcccgcc aatttgtatt
      301 cgtgtcaccc gtatggggtg agttctcact cacccctggc gcgttgcaat agcaggcggt
      361 ttatgcgcgc cgtggtgaca tagcgcccgg cgcgcacgtc gcgcagggcc cgggccgcga
      421 tctcgtcgcg cgggttcagc cgggtcaccc ggcgcagcag cgcggtcgcc tcggcccggt
      481 cgtcgcgctc ggcggcgatc acgcccagct ccagcagggc gtaggcgttc tcgggctcgc
      541 gcgccctggc ccggcggaag gaacgctcgg cctggtccag ccggcccagg cgcagggcga
      601 tcgtgcccgc cgacaggtcg gcctgggccg acatcgggtt cagcttgcgc gcgcggtcca
      661 ggcgcttgaa cgccccgtcc ggggatcccc gccaggcctt ggccgcgcgg tcgatctcga
      721 ggtcggcgat ccaggggccg gccaggaaga cggtggccac cgccacggcg acgcctccgg
      781 cggccagcac gcctgtcggc agcgaggacg tgggcagcgg gggccggggg acgggcgagt
      841 tgccgcgcgg cgccaggccg gcggccaggc ccagcagggc cagggccggg gcgcccagcc
      901 cggcgaactc ccagaaccag tccaccgagc cgtgcaggaa ccagtaggcg aaggccgccg
      961 cggcggcgcc tgccgccgcc ccgacgggcg cgtagccctt ggcgatcgcc cgcagcgccg
     1021 ccgccgccag cgccgccatg gcgccgaagg ccagcagcgc gccgaccagc ccggtctggg
     1081 ccagcacccc cagctgcagc gagtgcgcgt agcgggggcg ctccagggac ttgccctggc
     1141 gcaggtagta gggctggaag ccgtcggcgc ccacaccgcg gaccggatgc tcgccgaaca
     1201 ggtcccaggc gacgacccag aagtcgtagc ggttggtccc gcccgtcgag aagcgggact
     1261 ggccctgccc ggcctgggtg tccaggctct tgaactgctg ccagctgttg tccaggcgct
     1321 cgatcgggtt gcccgccttg gcccacgcgc cggcgcccag ggccaggacg atcacgatca
     1381 gcgcggcccc gacggcgcgc tcgcggcgcc tgcggaactc ctggccggcg ggcttgagcc
     1441 ggcgctcggc catcccggcc agcgtccccc cgaggcccag gagcgccgcg acgaccagga
     1501 tccccagcgc ggcctcggag gccatgtcgt cgatggcccc cgcgctgaac tcgtcgtgca
     1561 cggccaggag cgaggacgag atggccgcgg tgccggcggc cacggccagg acggccacca
     1621 gcgaccggcg ccagtggcgg ctggtcacca cgaagatcag gaccgcgaac gggatcgcca
     1681 ccagccagcc gcggctctgg cccatcagcg tcacgcccga gagcagcccc gcgcaggcca
     1741 ggaacaggcc ccggctcagc ggcgccacct cgcgcgccgc gcccaggtac aggcacggga
     1801 acgtggcgat gacccacatg gccacgttgg cgttgatgta ccccgcgggc tcgaagaagc
     1861 gggcgtcgtt gaggtagggc agcgggtcgg actggaactt caggagctgg accagggcga
     1921 tggccgagat gcccaggccg aacgtgccca gcaccagccg cgcgccgcgc gggttccagg
     1981 gccagaaggc gaacagcgcc aggacggtgg tgtagaagag cgtgcggttg gcgccgtcca
     2041 gcgcctggcc gttctcgccc gcccaggcga tggacaggta ctgccaggcg gcgaagccgg
     2101 ccatcagcag gaccccggcc aggaccgtgc gcggcacgtc gcgggggatg ccgacggcga
     2161 cgacggtggc caccagcagg gccagggtca gcagcgcgat gccgtaccag aggctggggt
     2221 agaacccggc ctcggagccg gccatggcca cgaaggccac catggccagc aggccggggc
     2281 ccagcgcggg gttgccctgc accagcaggc ggacggtgaa cagcaggcgt ccgaggggtg
     2341 ccgatagggt cgcggtcgtg gtcggcaaga tcgtctactg gctcgcggtg gtcgccatct
     2401 cactggcgct ggtcatcgcg ctcatactct tcttcgagtc gagggacgat tcctcgctgg
     2461 agggcgcggc cgtgcccgcc cccgtcgcgc tcacgggcgc ttgacgagcc tggcgaagac
     2521 cacgatccgc tgcgccgcgc tgtacagcgg gtggcaggcg gtcagcacca gccggtcgta
     2581 gccgacgttg tcggtcaccg aggtctcggt gggggcgacg atgcgggtct tctgcacctt
     2641 gtagacgtag gtgccgtacc gcatccgcag gtcgatctgg tcgcctcgct tgagcttgtc
     2701 gatggtccgg aacggcgccc cgtaggtggt gcggtgcccg gcgatggcca cggtcttgct
     2761 ctggccgggg agcgcggtgt cggggtagtg gcccggtccc ttgcgcaggt cggtcgtctt
     2821 ggtgccctcg atcacgtagt acttgcggtc cagggtcggg aagctcagct gggccacggc
     2881 cgagccgggg atcggcttct gcttcggcgg ccggccctcc ttcggcttcg gctggctctc
     2941 gggcgtggcg gcgaacgcct cctcgaggtc gttctgctgg cgctgggcca cgaacgcgct
     3001 gaccggctcc tgccacagaa gggtggcgcc ggcgtcggcg atcatcagca ccccgctgac
     3061 catcaggacc gaagccacga accggaggac cgccttcatc gcgtcaggtc agtggttgtc
     3121 gtcgcggccg ccggcggccg gtgggcgtga ctcttcggcc agcgacgtca gctccagcgt
     3181 gccgcgggcg gccgaggtca gcacctcgct ggcctcgtcg gaggagcgcg cgtacagctc
     3241 gatgagcatg tggatggcga tctggcccga gtcgtcgtgc ttgtggaagc gcacgtgggt
     3301 gaagaactcg cgcgtgccgt ggtcgccgaa cgcggcgaag gcaagcgagt ccgcggcctc
     3361 cgggccggag caggactcga cctcgctctc gtccaccgcc acggtgcagg aggcgaccca
     3421 gggcgtgccc gtgaccatgc gctcccagcg gtcctcgacg gcctcgatcc ggctcacccg
     3481 gcggccgtcc tcctcgtaga aggtcaggct caggtggggc tggtcgtgca tgcagtccat
     3541 gcactggacc acggcctcct ggacctcgtc gatgggctcg acggcctcgg cggtcgcggg
     3601 atcgatcttc aggatcccct cgtagtgca
//
LOCUS       DASUPW010000170         4715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1009838, whole genome shotgun sequence.
ACCESSION   DASUPW010000170 DASUPW010000000
VERSION     DASUPW010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009838"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1601)
                     /locus_tag="VFY44_03900"
     CDS             complement(<1..1601)
                     /locus_tag="VFY44_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909610.1"
                     /translation="MRRRLSGALPVVLLVVFIALPALSATGQLPQLNDLFALGKGEGQ
                     TPAKAPVQTNPEPPLVATPRANCVSGGPREPGIQGRVPANAAANGLSCNTTLIGRAGN
                     SGGFKVHRYVDRAGHECAYYDTALLFPTNAVRLDGTSGGVAVLDMSNPAKPVETARLT
                     ELPMLSPHESVELNTKRGLLAAVLGNPSTYPGLVSIYDVSQDCRKPVLQSTASVARLG
                     HESGFSEDGKTFYATGTAVETISAIDVTDPKQPKNIWQGAVTSHGMSLRPDGNRAYLA
                     DPGGQLVTLDTSEIQARKPNPQAREISRLTWKSASIPQNALPFTKDGKPYILEFDEYT
                     AGTSGGGNADEVGAGRIIDMSDERKPRVIAQLRLQVNQPADHAAAREDPGALSPVQGY
                     AAHYCDLDSRVDPKVVACSFITSGLRVFDVTELTKPKEIGYFVAPTKGRTENGYMASS
                     FAMSKPEIVGDRREVWYSDGATGFYNVRVDERVWPSSSSAATGRGCLARRSPVGPRNF
                     GRIRLGMTRKTLARRLPDPVRRTKRS"
     gene            1692..3104
                     /locus_tag="VFY44_03905"
     CDS             1692..3104
                     /locus_tag="VFY44_03905"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5909611.1"
                     /translation="MGHETHDDFETQFWLNTVTAGAWITVLMCVAGGAYTVFFVEAPD
                     RLAVGASIVVTAIGGVIVRWGIPWRSLIGSRWRELAFGTWTALTIVLIATSAALDGGA
                     TSPLALMLFLPVVFASLAYPLRLVLTSALLAVAAFLALVLVAAPSTAYVAAFCLALAG
                     TALMALWQAANHDGWRHELARSSRTDPLTGLLNRRGFAAASEAAFSALERHGRPVTLV
                     VLDLDLFKAYNDTRGHQAGDELLCWVAGRLEGEMRPSDAVARLGGDEFAVLLPDTGQA
                     VAERVIARLRAALEPRARHCLGRASAPEQGTTFDSLYRIADTDMYQRKLLRLADPLDE
                     ESTEAAFEHRKRSRALSADDVLAGITDAFFALDGQWRFVYVNEPAERLLEQPAHELLG
                     RSIWSQFPEAVGSRFDRIYRHVASTGQPQLFAERYGPLERTFSVKASPLNDGISVYFH
                     DVSGEPETRTSAVDPAAAGA"
     gene            complement(3142..4521)
                     /locus_tag="VFY44_03910"
     CDS             complement(3142..4521)
                     /locus_tag="VFY44_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5909612.1"
                     /translation="MIFRQIIHEDLGSAAYLVGDEGTGRAALVDPALQVDEYLRQLRY
                     AGLTLEHIVETHTHADRVSGHGQLAAATGATIHIHRIAGVEYEHEPFEDGWELELGRV
                     VLRALHTPGHRPEHTSFLVTDRARGDEPWAVLTGDALFVADIGRPDLAVEPEEGARDL
                     FSSLHDKLLTLPPYVEVWPGHLGGSLCGGPGMDRKVSSTIGYEREHNPVLAHSDRDRF
                     VADAIDGLAPQPPRFERIVRVNSGPLQSRPVAPGPLSPRQLEARRDAGALVVDVRTDQ
                     QFDDAHIPGAVSNTALRAGFGTKLAWLVEEGQEVVLVGRDDHDALAAADLAASVDVTA
                     VSGYLAGGMTSWREDQRPVERIERIDIDTLHERWEGDQAPQVLDVRDPDEWEAGHLPG
                     SLHRPYHAIHAVPDGLDPDRPVAVLCASGQRAGVGASLLQHFGVAEVAHVAGGGVGTW
                     ERRGWPVER"
     gene            complement(4518..>4715)
                     /locus_tag="VFY44_03915"
     CDS             complement(4518..>4715)
                     /locus_tag="VFY44_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909613.1"
                     /translation="GSAMFGLGWAIADTCPGPVAAQLGQGLPWALCTAAGITGGVLLF
                     QARRRTAAPASAPATTATGRA"
BASE COUNT          589 a         1714 c         1734 g          678 t
ORIGIN      
        1 gagcgcttgg tcctgcgcac ggggtcgggc aggcggcggg ccagcgtctt cctggtcatg
       61 cccaggcgga tccggccgaa gttcctgggc cccaccggcg accggcgcgc cagacagccc
      121 cggccggtgg ccgcgctgga ggaggacggc cagacgcgct cgtcgacgcg gacgttgtag
      181 aagccggtcg cgccgtcgga gtaccagacc tcgcgccggt cgccgacgat ctcgggcttg
      241 gacatcgcga agctgctggc catgtagccg ttctccgtcc ggcccttggt gggcgccacg
      301 aagtaaccga tctccttcgg cttggtcagc tcggtcacgt cgaacacgcg caggccggag
      361 gtgatgaacg agcacgcaac gaccttcggg tccacgcgcg aatccaggtc gcagtagtgc
      421 gccgcgtagc cctggacggg gctgagggcc ccggggtcct cgcgcgcggc cgcgtggtcg
      481 gccggctggt tgacctgcag gcgcagctgg gcgatcaccc gcggcttgcg ctcgtcggac
      541 atgtcgatga tgcggcccgc gcccacctcg tcggcgttgc cgccgccgct cgtgcccgcg
      601 gtgtactcgt cgaactccag gatgtacggc ttgccgtcct tggtgaacgg cagcgcgttc
      661 tgggggatcg acgccgactt ccatgtcagg cggctgatct cccgcgcctg cgggttgggc
      721 ttgcgcgcct ggatctcgct ggtgtccagc gtgaccagct ggccgccggg atcggccagg
      781 taggcccggt tgccgtcggg ccgcagcgac atgccgtgtg aggtgaccgc tccctgccag
      841 atgttcttcg gctgcttggg gtcggtcacg tcgatggcgg agatggtctc gacggcggtg
      901 cccgtggcgt agaaggtctt cccgtcctcg gagaacccgc tctcgtggcc caggcgggcg
      961 accgaggcgg tggactggag caccggcttg cggcagtcct ggctgacgtc gtagatggac
     1021 accaggcccg ggtaggtcga ggggttgccc agcacggcgg ccagcaggcc gcgcttggtg
     1081 ttcagctcca ccgactcgtg cgggctgagc atgggcagct cggtcagcct ggccgtctcg
     1141 accggcttgg cggggttgga catgtccagc acggccacgc cgccggaggt gccgtccagc
     1201 ctgaccgcgt tggtcgggaa cagcagcgcg gtgtcgtaat aggcgcactc gtggccggcc
     1261 cggtccacgt accggtgcac cttgaacccg cccgagttgc cggcgcgccc gatcagggtg
     1321 gtgttgcagc tcagcccgtt cgcggcggcg ttggcgggga cccggccctg gatgcccggc
     1381 tcgcgcggac cgcccgagac gcagttggcc cgcggcgtgg ccacgagcgg cggttcgggg
     1441 ttggtctgca ccggggcctt ggcgggggtc tggccctcgc ccttgccgag cgcgaacagg
     1501 tcgttcagct gcggcagctg gccggtcgcg ctcagcgcgg gaagcgcgat gaacaccacc
     1561 agcaacacca ccggcagggc ccctgagagt ctcctccgca ccgccgcgat gctacaccgg
     1621 caccggcccc accgcgccgg tccgcggggg attcctcaag ggacggtcgg gggcgtcgat
     1681 agaccgagta catgggtcac gagacgcacg acgacttcga gacccagttc tggctgaaca
     1741 cggtcacggc aggggcatgg atcaccgtgc tcatgtgcgt cgcgggcggg gcctacaccg
     1801 tcttcttcgt cgaggcgccg gaccggctcg cggtcggcgc gtcgatcgtg gtcacggcga
     1861 tcgggggcgt gatcgtgcgc tggggcatcc cgtggcgctc cctgatcggc tcgcgctggc
     1921 gcgagctcgc gttcgggacc tggacggccc tgaccatcgt gttgatcgcg acctccgccg
     1981 cgctggacgg gggcgccacg agcccgctcg cgctgatgct gttcctgccg gtggtcttcg
     2041 cctcgctggc ctatcccctg cggctggtgc tcacgtcggc gctgctggcc gtggcggcct
     2101 tcctggcgct ggtgctcgtc gcggccccca gcaccgccta cgtggccgcg ttctgcctgg
     2161 cgctggccgg caccgcgctg atggccctgt ggcaggcggc caaccacgac gggtggcggc
     2221 acgagctcgc acgcagctcg cgcaccgacc cgctgaccgg gctgctgaac cggcgtgggt
     2281 tcgcggcggc gtccgaggcc gcgttcagcg cgctcgagcg ccacggcagg ccggtgaccc
     2341 tcgtggtcct ggacctggac ctgttcaagg cctacaacga cacccgcggc caccaggccg
     2401 gcgacgagct gctgtgctgg gtcgcgggca ggctggaggg cgagatgcgc ccctcggacg
     2461 cggtggcccg cctgggtggg gacgagttcg ccgtgctgct cccggacacc gggcaggcgg
     2521 tcgcggagcg cgtgatcgcc cgcctcaggg ccgccctgga gccccgtgcg cggcactgcc
     2581 tgggccgggc cagcgccccc gagcagggaa ccacgttcga ctcgctgtac cgcatcgccg
     2641 acaccgacat gtaccagcgc aagctcctgc gcctggcgga tccgctggac gaggaatcca
     2701 cggaggccgc cttcgagcac cgcaagcgca gcagggcgct ctcggccgac gacgtcctgg
     2761 ccggaatcac cgacgcgttc ttcgcgctgg acggccagtg gcgcttcgtg tacgtgaacg
     2821 agcccgcgga gcggctgctg gaacagccgg cgcacgagct gctcggccgc tcgatctggt
     2881 cacagttccc tgaggccgtg ggctcgcggt tcgaccgcat ctaccgccac gtggcctcca
     2941 ccggccagcc ccagctgttc gccgagcgct acggcccgct ggagcggacg ttctcggtca
     3001 aggcgagccc actgaacgac ggcatctcgg tctacttcca cgacgtctcc ggcgagccgg
     3061 agaccagaac ctccgcggtg gacccggcgg ccgccggcgc ctagtcgacc gggggccggc
     3121 acgcccggcc gcgcacgcac atcaccgctc gacaggccag ccgcgccgct cccacgtgcc
     3181 cacgccgcca ccggccacgt gggcgacctc cgcgacgccg aagtgctgga gcagcgaggc
     3241 gccgacgccg gcgcgctgac ccgaggcgca cagcaccgcg acgggccggt ccgggtccag
     3301 gccgtccggg acggcgtgga tggcgtggta gggccggtgc agcgaccccg gcaggtgccc
     3361 ggcctcccac tcgtccgggt cgcggacgtc cagcacctgc ggggcctggt cgccttccca
     3421 ccgctcgtgc agcgtgtcga tgtcgattcg ctcgatccgc tcgaccggcc gctgatcctc
     3481 gcgccagctg gtcatgccgc cggccaggta gcccgagacg gcggtcacgt ccaccgacgc
     3541 ggccaggtcc gccgcggcca gcgcgtcgtg gtcgtcgcgc ccgaccagca cgacctcctg
     3601 gccctcctcg accagccagg ccagcttggt gccgaacccg gcgcgcagcg cggtgttgga
     3661 caccgcgccg gggatgtggg catcgtcgaa ctgctggtcg gtgcggacgt ccaccaccag
     3721 cgcgccggcg tcccggcgcg cctcgagctg gcgtgggctc aggggcccgg gcgccaccgg
     3781 gcgtgactgc agcggccccg agttcacccg cacgatgcgc tcgaagcggg gcggctgggg
     3841 cgccagcccg tcgatggcgt cggccacgaa gcggtcgcgg tcggagtggg ccagcaccgg
     3901 gttgtgctcg cgctcgtagc cgatggtgga ggacaccttg cggtccatgc ccggaccgcc
     3961 gcacagggac ccgcccaggt ggccgggcca cacctcgacg tacggcggca gggtcaggag
     4021 cttgtcgtgc agcgagctga acaggtcgcg ggcgccctcc tcgggctcca ccgccaggtc
     4081 gggacggccg atgtccgcca cgaacagggc gtcgccggtg agcaccgccc acggctcgtc
     4141 accgcgagcg cggtccgtga ccaggaagga ggtgtgctcg ggccggtggc cgggtgtgtg
     4201 cagcgcccgc agcaccaccc gccccagctc gagctcccag ccgtcctcga acggctcgtg
     4261 ctcgtactcg acgcccgcga tccggtggat gtggatcgtc gcgccggtcg cggcggccag
     4321 ctgaccgtgc cctgacaccc ggtcggcgtg ggtgtgggtc tccacgatgt gctccagcgt
     4381 caggccggcg tagcgcagct ggcgcaggta ctcgtccacc tgcagcgccg ggtcgaccag
     4441 ggccgcccgc cccgtgcctt cgtcgcccac caggtacgcg gcggagccca ggtcctcgtg
     4501 gatgatctgg cggaagatca cgcccggccg gtggccgtgg tcgcgggcgc gctcgcgggc
     4561 gccgccgtgc ggcgccgggc ctggaacagc agcaccccgc cggtgatccc ggccgcggtg
     4621 cacagcgccc acgggagccc ctggcccagc tgggccgcca ccggccccgg gcaggtgtcg
     4681 gcgatggccc accccaggcc gaacatcgcg ctgcc
//
LOCUS       DASUPW010000171         4535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_179068, whole genome shotgun sequence.
ACCESSION   DASUPW010000171 DASUPW010000000
VERSION     DASUPW010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179068"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..1196)
                     /locus_tag="VFY44_03920"
     CDS             complement(51..1196)
                     /locus_tag="VFY44_03920"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX5909614.1"
                     /translation="MALSALQELEARAVVGTYARNPVEFVRGQGTTLWDADGNEYLDF
                     LAGISVAQIGHCHPALVEAVTEQAGRLMHVGNLFYTEPSMRLAGRLSDLALGGKAFFC
                     NSGTEAVECAIKLARRHRPKGDFVVMERGFHGRTMGALSATPQEAKQAPFAPLVPGFK
                     VVPHDDAEALAAAVDGHTAAVLIEPVQGEGGIHPLSDATLAAARTACDEHGAMLIFDE
                     IQCGMGRTGDWWGWQRSGIRPDAMTVAKGLGGGLPIGACLTAPAYADVLQPGDHGSTF
                     AGGPVIAAGANAVLDVISEDGFLGDVAAKGARLSDALVGLGLQPRGRGLMLAFAAGDA
                     PALALRLLTEHRLVVNATGPDTIRLLPPLTVSEEEIDEAAARISASL"
     gene            complement(1187..2029)
                     /gene="argB"
                     /locus_tag="VFY44_03925"
     CDS             complement(1187..2029)
                     /gene="argB"
                     /locus_tag="VFY44_03925"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX5909615.1"
                     /translation="MRDVATLLEALPYIREFHGRTVVIKYGGAAMVDPVLREEFARDV
                     VLLKYVGLNPVVVHGGGPAITGYMERLGMEVEFVDGLRVSDAATVEVAKMVLVGKQNK
                     DIVLLLGRHGQPAVGLCGDDGMLFQVRKQERADGRDIGFVGEIERVNVGVLNHIAEDY
                     IPVIASVGADPEGQSYNVNADAAAAAVASALGATKCIFLTDVVGWLSDPEDPESRIST
                     GTADDVEKALATVQGGMRPKLEACVQALRGGVQNAYIIDGRRPHALLLELFTDEGIGT
                     KLWP"
     gene            complement(2039..3286)
                     /gene="argJ"
                     /locus_tag="VFY44_03930"
     CDS             complement(2039..3286)
                     /gene="argJ"
                     /locus_tag="VFY44_03930"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX5909616.1"
                     /translation="MTAPFFRSKWVDRPEHATELAPTALPEGFVAGGVAAGIKPEGED
                     VGLLFSEREATTSSARFTTNALVAAPVTVAREADLSALRAITVNSGNANVSDGERGLE
                     TARAQQRAAAEALDLNPAHVAVASTGVIARELPRDKAVAGIRHAAQSLAPNAQAFSES
                     ILTTDKGPKRACLELALPSGGTVRLAGQAKGGGMISPHFATMLCFVQTDAALEAETVE
                     LLTRVCVKRSFDRISVDGQLSTNDAVFVQANGASGVQVQPESPDELAVGEALDALLRQ
                     LALEMVADGEGASRVGRVVVRGAPESVGPVARAVANSPLVKTALAGADPNFGRILQAV
                     GQGLAGRAPFDIDLAIDGLPVVRGGAMVELDYPDDWVRVEASFAADELEFDLKVPGDG
                     GEAEVFFSDLTHEYVTINADYTT"
     gene            complement(3283..4251)
                     /gene="argC"
                     /locus_tag="VFY44_03935"
     CDS             complement(3283..4251)
                     /gene="argC"
                     /locus_tag="VFY44_03935"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX5909617.1"
                     /translation="MIGAAGYGGALGAGIVQRHPGLELTAVTARSDAGRRHDELYPIH
                     RIGLVMDEFDPDRVAELADSALVAYPHKAAAGAVRELRARGLKVVDFSADFRLGQDAY
                     EHWYQPHEAPELLGEAVYGLPEINGHRDQIREAELVAGPGCNSTAALLGLWPLRDHLR
                     DAVVDIKTGVSGAGREATQETHYVSASDNVNAYKTEGHRHQAELEQELPAGVGITFVP
                     HLIPVDQGLLATNYALTDGDLTREDVRELLHAAYDGEPFVDVVDSPPHTAHVRGTNRA
                     HVYGTVTGNRVLVESAIDNLWKGAAGQAVQDLNLMLGLDETEGLDR"
     gene            complement(4259..>4535)
                     /locus_tag="VFY44_03940"
     CDS             complement(4259..>4535)
                     /locus_tag="VFY44_03940"
                     /inference="COORDINATES: protein motif:HMM:NF015129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909618.1"
                     /translation="PAVRQDIAVVTPAGVEAEAIRLTVLDAGGELLTGARVFDVYEGE
                     QVGEGNRSLALALEFRAPDRTLTDEEVAGPRAAIEGALAEKGWSLRA"
BASE COUNT          581 a         1688 c         1635 g          631 t
ORIGIN      
        1 gacggccgcc agggtcgcga ggcgcgggga cccgtgatga agaccaccgg ctagagcgac
       61 gcgctgatcc gcgccgccgc ctcgtcgatc tcctcctcgg agaccgtgag cggcggcagc
      121 agccggatcg tgtccggccc ggtggcgttc accacgaggc ggtgctcggt gaggaggcgc
      181 agcgccagtg ccggcgcgtc gccggccgcg aaggccagca tcaggccgcg cccgcgcggc
      241 tgcaggccca ggccgaccag cgcgtcggac agccgggcgc ccttggccgc gacgtccccg
      301 aggaagccgt cctcggagat cacgtccagc accgcgttgg cgccggccgc tatcaccggc
      361 ccgccggcga aggtggagcc gtggtcgccg ggctggagca cgtcggcgta ggccggcgcc
      421 gtgaggcagg cgccgatcgg cagcccgccg cccagcccct tggccaccgt catcgcgtcg
      481 gggcggatcc ccgagcgctg ccagccccac cagtcgccgg tgcggcccat gccgcactgg
      541 atctcgtcga agatgagcat cgcgccgtgc tcgtcgcacg ccgtccgggc ggcggccagg
      601 gtggcgtccg acaacgggtg gatgccgccc tcgccctgca cgggctcgat cagcacggcg
      661 gcggtgtggc cgtcgacggc cgcggccagc gcctcggcgt cgtcgtgggg cacgactttg
      721 aagcccggta cgagcggcgc gaacggcgcc tgcttggcct cctggggcgt ggccgacaag
      781 gcgcccatgg tgcggccgtg gaaaccgcgc tccatgacca cgaagtcccc cttcgggcgg
      841 tgacggcggg ccagcttgat ggcgcactcg acggcctcgg tgcccgagtt gcagaagaac
      901 gccttgccgc ccagcgccag gtccgacagg cgcccggcca ggcgcatcga gggctcggtg
      961 tagaacaggt tgcccacgtg catcaggcgc ccggcctgct cggtcacggc ctccaccagc
     1021 gccggatgac agtggccgat ctgcgccacc gagatgccgg ccaggaagtc caggtactcg
     1081 ttgccgtcgg cgtcccagag ggtggtgccc tggccgcgga cgaactcgac cgggtttcgc
     1141 gcgtacgtgc ccaccacggc acgcgcctcc agctcctgca gcgcgctcag ggccacagct
     1201 tggtcccgat gccctcgtcg gtgaacagct ccagcagcag cgcgtgcggg cgccggccgt
     1261 cgatgatgta cgcgttctgc acgcccccgc gcagggcctg gacgcaggcc tcgagcttgg
     1321 gccgcatgcc gccctggacg gtggccagcg ccttctccac gtcgtcggcg gtgcccgtcg
     1381 agatccgcga ctcggggtcc tcggggtccg agagccagcc cacgacgtcg gtcaggaaga
     1441 tgcatttggt ggcgccgagg gcggaggcga cggcggccgc cgccgcgtcg gcgttcacgt
     1501 tgtagctctg cccttcgggg tccgcgccca cggaggcgat caccgggatg tagtcctcgg
     1561 cgatgtggtt cagcacgccc acgttcacgc gctcgatctc gcccacgaag ccgatgtcgc
     1621 gcccgtccgc gcgctcctgc ttgcggacct ggaacagcat gccgtcgtcg ccgcacaggc
     1681 ccactgccgg ctgcccgtgg cggcccagca gcagcacgat gtccttgttc tgcttgccga
     1741 ccagcaccat cttggccacc tccacggtgg ccgcgtcgga cacgcgcagg ccgtccacga
     1801 actccacctc catgcccagg cgctccatgt agcccgtgat ggcggggccg ccgccgtgca
     1861 ccaccacggg gttcaggccc acgtacttga gcagcaccac gtcgcgcgcg aactcctcgc
     1921 gcagcaccgg gtcgaccatg gcggcgccgc cgtacttgat gaccaccgtg cgcccgtgga
     1981 actcgcggat gtagggcagc gcctcgagga gtgttgcgac gtctctcatg cgtggtccct
     2041 aggtcgtgta gtcggcgttg atggtcacgt actcgtgcgt caggtcgctg aagaagacct
     2101 cggcctcgcc gccgtcgccc ggcaccttga ggtcgaactc cagctcgtcg gcggcgaacg
     2161 acgcctccac gcggacccag tcgtccgggt agtcaagctc caccatcgcg ccgcccctga
     2221 ccacgggcag gccgtcgatg gccaggtcga tgtcgaacgg ggcgcggccg gccaggccct
     2281 ggccgacggc ctgcaggatg cggccgaagt tggggtcggc gccggccagc gcggtcttga
     2341 ccagcgggga gttggccacc gcgcgcgcca ccggccccac cgactccggt gctccgcgca
     2401 cgaccacgcg ccccacgcgg gacgcgccct cgccgtcggc caccatctcc agggccagct
     2461 ggcgcagcag ggcgtccagg gcctcgccca ccgccagctc gtccggcgac tcgggctgga
     2521 cctgcacgcc ggacgcgccg ttggcctgca cgaacacggc gtcgttggtg gacagctgac
     2581 cgtccaccga gatccggtcg aagctgcgct tcacgcacac gcgggtcagc agctcgacgg
     2641 tctccgcctc cagcgcggcg tcggtctgga cgaagcacag catcgtcgcg aagtgcggcg
     2701 agatcatgcc cccgcccttg gcctggccgg ccaggcggac cgtgccgccg gaaggcagcg
     2761 ccagctccag gcacgcgcgc ttggggccct tgtctgtggt caggatcgac tccgagaagg
     2821 cctgggcgtt gggggccagc gactgggcgg cgtggcggat cccggccacg gccttgtcgc
     2881 gcggcagctc gcgcgcgatc acgcccgtgg aggccacggc gacgtgcgcc gggttcaggt
     2941 ccagcgcctc ggcggcggcc cgctgctggg cgcgagcggt ctccaggccg cgctcgccgt
     3001 cggacacgtt ggcgttgccc gagttcacgg tgatcgcgcg cagggcgctc agatcggcct
     3061 cacgggccac ggtcaccggc gcggccacca gcgcgttggt ggtgaagcgg gcggacgagg
     3121 tggtggcctc gcgctcggag aacagcaggc cgacgtcctc gccctcgggc ttgatcccgg
     3181 ccgccacgcc gccggcgacg aagccctcgg gcagcgcggt cggggccagc tcggtcgcgt
     3241 gttcgggccg gtcgacccac ttcgagcgga agaacggcgc ggtcaccggt ccaggccctc
     3301 ggtctcgtcc aggcccagca tcaggttcag gtcctgcacg gcctggcccg cggcgccctt
     3361 ccagaggttg tcgatggccg actcgaccag cacgcggttg ccggtcacgg tcccgtacac
     3421 gtgcgcgcgg ttggtgccgc gcacgtgcgc ggtgtgcggc gggctgtcca ccacgtccac
     3481 gaacggctcg ccgtcgtagg cggcgtgcag cagctcgcgc acgtcctcgc gggtcaggtc
     3541 gccgtcggtg agcgcgtagt tcgtggccag caggccctgg tcgaccggga tcaggtgggg
     3601 cacgaacgtg atcccgaccc cggccggcag ctcctgctcc agctcggcct ggtggcggtg
     3661 cccctcggtc ttgtaggcgt tcacgttgtc cgaggccgac acgtagtggg tctcctgggt
     3721 ggcctcgcgg ccggcgccgg agacgccggt cttgatgtcc acgacggcgt cgcgcaggtg
     3781 atcgcgcagc ggccacaggc ccagcagcgc ggcggtcgag ttgcagccgg gcccggcgac
     3841 gagctcggcc tcgcggatct ggtcgcggtg gccgttgatc tcgggcaggc cgtacacggc
     3901 ctcgcccagc agctcgggtg cctcgtgcgg ctggtaccag tgctcgtagg cgtcctggcc
     3961 cagccggaag tcggccgaga agtccaccac cttcaggccc cgcgcgcgca gctcgcgcac
     4021 cgcgccggcg gcggccttgt gcgggtaggc caccagcgcc gagtcggcca gctccgccac
     4081 ccggtcgggg tcgaactcgt ccatgaccag gccgatgcgg tggatcgggt agagctcgtc
     4141 gtggcgcctg ccggcgtcgc tgcgcgcggt gaccgcggtc agctccaggc ccgggtggcg
     4201 ctgcacgatg ccggcgccca gcgcgccgcc gtagccggcg gctcctatca cggcgaccct
     4261 atgcacggag ggaccacccc ttctccgcga gtgcgccttc gatggcggcg cgcggccccg
     4321 cgacctcctc gtcggtgagc gtccggtcgg gggcacggaa ctccagggcc agcgccagcg
     4381 agcggttgcc ctcccccacc tgctcgccct cgtacacgtc gaacacgcgc gcgcccgtga
     4441 gcagctcgcc gccggcgtcc aggaccgtca gccggatggc ctccgcctcc acgcccgccg
     4501 gggtgaccac cgcgatgtcc tgccggacgg ccggg
//
LOCUS       DASUPW010000172         2647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_594625, whole genome shotgun sequence.
ACCESSION   DASUPW010000172 DASUPW010000000
VERSION     DASUPW010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594625"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VFY44_03945"
     CDS             <1..548
                     /locus_tag="VFY44_03945"
                     /inference="COORDINATES: protein motif:HMM:NF015279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909619.1"
                     /translation="MDASGKDKVLLETVGVGQAEVDIIDHADTVVLCLMPGSGDSIQA
                     LKAGVMEIPDIIVVNKMDHPLTDTMVREVRGVLSLGDQTGGWRVPIVKTEAARGEGVE
                     ELYEKMTEHRAFIQSEGTLAERRRRNLMNEVMALTAGRLQRRLEESLAEDESVQKLLD
                     RVVAREIDPSTAATELLEREL"
     gene            800..982
                     /locus_tag="VFY44_03950"
     CDS             800..982
                     /locus_tag="VFY44_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909620.1"
                     /translation="MPTVTVKVKKKCCKSGPRCKKCPAVYKKLSNQGHAEQLSKRLYV
                     VEGKVPKKAMKLARKR"
     gene            1120..1989
                     /locus_tag="VFY44_03955"
     CDS             1120..1989
                     /locus_tag="VFY44_03955"
                     /inference="COORDINATES: protein motif:HMM:NF020932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EfeM/EfeO family lipoprotein"
                     /protein_id="HEX5909621.1"
                     /translation="MALGAGACGEDRGNVDVEGSTTGGSKTGTTAATVDRSKTDPKQL
                     AAAMSKVKAYAVEQTGALVTSTQKLQQAIDSGDVDAAKKAYEKARPYYERIEPLVVLF
                     PELDGKIDAREDDFPKKAEDPNWTGFHPIERDLWKDEKITAATEKLAAGLYEDSTRLN
                     DLMVKAVVKPEVVIPGTAELIDEVEESKITGEEERYSKLDLPTFVANLDGAKAFYETL
                     SPLVKAKDPSLDKQIQEAFDDAYAEVNKLKKGGDFVSYDELTDAQQKEVKQTIEALAE
                     PLARVQGVLGVDK"
     gene            1986..>2647
                     /locus_tag="VFY44_03960"
     CDS             1986..>2647
                     /locus_tag="VFY44_03960"
                     /inference="COORDINATES: protein motif:HMM:NF016173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase domain-containing protein"
                     /protein_id="HEX5909622.1"
                     /translation="MTDTSRRRFLQGAAGGGAAVAAGAFGVSRLTDADEAEAKERLLS
                     WKGPRQAGIVSERTPYGMIAAFDVVTDDLALLMRDLTARVEELTQGWPDRLDDLTNES
                     LPPSDTGELGYDRRKDGRLSITVGFGDALFDDRFGLADKKPPALKKMPSFPVDNLDPK
                     RTHGDIVILVQSDHMMVTHHAMRDIMRRTKGRLEGRWAQACFQRIDEKKEGRPKIAAH
                     RGL"
BASE COUNT          475 a          884 c          946 g          342 t
ORIGIN      
        1 tcatggacgc ctccggcaag gacaaggtcc tgctggagac cgtgggggtg gggcaggccg
       61 aggtggacat catcgaccac gccgacaccg tggtgctgtg cctgatgccc ggctcgggtg
      121 actcgatcca ggcgctgaag gccggggtca tggagatccc ggacatcatc gtggtcaaca
      181 agatggacca cccgctgacc gacacgatgg tgcgcgaggt ccgcggcgtc ctgtcgctgg
      241 gcgaccagac cggcggctgg cgcgtgccca tcgtcaagac cgaggccgcc cgcggcgagg
      301 gcgtcgagga gctctacgag aagatgaccg agcaccgcgc gttcatccag tccgagggca
      361 cgctggccga gcgccggcgg cgcaacctga tgaacgaggt catggccctg accgcgggcc
      421 gcctccagcg ccgcctggag gagtcgctgg ccgaggacga gtcggtgcag aagctgctgg
      481 accgggtggt ggcgcgggag atcgatccaa gcaccgcggc caccgagctg ctcgagcgcg
      541 agctctgacg cgcgccggcg ttctgacgtc ggccccacca gttgcccggc gggaacggcg
      601 gctgggtggg gccggacgct cgcgcacgtg cgggcccacg tgatgctcgg gacggtttgg
      661 gccgggcggt gcacgccggc gcgcatctcc ccgcccatga ggctctcggc tccgcgtggg
      721 ccgggatctg cgcccctccg gacgtccgtt tgcaggcagt tagggccacc taagccaacc
      781 ctgctacctt gcgccgcccg tgccgaccgt caccgtcaag gtcaagaaga agtgctgcaa
      841 gtccggcccg cgctgcaaga agtgcccggc ggtctacaag aagctgtcca accagggaca
      901 cgcggagcag ctgagcaagc gcctgtacgt cgtcgagggc aaggtgccca agaaggcgat
      961 gaagctggca cgcaagcgct gatctgtctc cagaccccgc gcggagcggg gttaggctag
     1021 cctaagcacg ttctttcttg ggcatccctt atggacggag gcggtttgga acgcaggcgc
     1081 tgggcgatcg ttgcgagcag tgcggcactg ggagtcggtg tggccctggg cgccggggcg
     1141 tgtggcgagg accgcggcaa cgtcgacgtc gagggctcga cgaccggcgg cagcaagacc
     1201 ggcaccacgg cggccaccgt cgaccgcagc aagaccgacc ccaagcagct ggccgcggcc
     1261 atgtccaagg tcaaggccta cgccgtcgag cagacgggcg cgctggtcac ctccacccag
     1321 aagctccagc aggcgatcga ctcgggcgac gtggacgccg cgaagaaggc ctacgagaag
     1381 gcgcggccct actacgagcg catcgagccg ctggtggtgc tgttccccga gctggacggc
     1441 aagatcgacg cccgcgagga cgacttcccc aagaaggccg aggacccgaa ctggaccggc
     1501 ttccacccga tcgagcgtga tctgtggaag gacgagaaga tcaccgccgc caccgagaag
     1561 ctggccgcgg gcctgtacga ggactccacg cgactgaacg acctgatggt caaggccgtg
     1621 gtcaagcccg aggtcgtcat ccccggcacc gccgagctga tcgacgaggt cgaggagtcc
     1681 aagatcaccg gcgaggagga gcgctactcc aagctcgacc tgccgacctt cgtggccaac
     1741 ctggacggcg ccaaggcctt ctacgagacc ctctcgccgc tggtgaaggc gaaggacccg
     1801 tcgctggaca agcagatcca ggaggcgttc gacgacgcct acgccgaggt gaacaagctc
     1861 aagaagggcg gcgacttcgt ctcctacgac gagctcaccg acgcccagca gaaagaggtc
     1921 aagcagacca tcgaggcgct tgccgagccg ctggcccgcg tacagggtgt gctcggcgtg
     1981 gacaagtgac cgacacctcc cgacggcgct tcctccaggg agcggccggg ggaggggcgg
     2041 cggtggccgc gggcgcgttc ggcgtctcgc ggctcaccga cgccgacgag gcggaggcga
     2101 aggagcgcct gctgtcgtgg aagggcccgc gccaggccgg catcgtgagc gagcgcacgc
     2161 cgtacggcat gatcgccgcc ttcgacgtgg tcaccgacga cctggcgctg ctgatgcgtg
     2221 acctgaccgc gcgggtcgag gagctgaccc agggctggcc cgaccgcctg gacgacctga
     2281 ccaacgagtc gctgccgccg tcggacacgg gcgagctggg ctatgaccgg cgcaaggacg
     2341 gccgcctgag catcaccgtc ggcttcggcg acgcgctgtt cgacgaccgc ttcgggctgg
     2401 cggacaagaa gccgccggcg ctgaagaaga tgccgtcgtt cccggtggac aacctggacc
     2461 ccaagcgcac ccacggcgac atcgtgatcc tggtccagtc cgaccacatg atggtcaccc
     2521 accacgcgat gcgggacatc atgcgccgca ccaaggggcg cctcgagggc cgctgggccc
     2581 aggcctgctt ccagcgcatc gacgagaaga aggaggggcg gccgaagatc gccgcccacc
     2641 gcggcct
//
LOCUS       DASUPW010000173         2618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1149139, whole genome shotgun sequence.
ACCESSION   DASUPW010000173 DASUPW010000000
VERSION     DASUPW010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149139"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1498
                     /locus_tag="VFY44_03965"
     CDS             <1..1498
                     /locus_tag="VFY44_03965"
                     /inference="COORDINATES: protein motif:HMM:NF016859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909623.1"
                     /translation="MRGLMTDPTGKVIELPIRSSFREGLTVLEYFISTHGARKGLADT
                     ALRTADSGYLTRRLVDVAQDVIVREEDCGTKEGIDMPLFGEDGKPNENLLGRHTSVKF
                     GDLKKGELIAKPSLAALVEAAGDDEAATVPVRSVLKCEADTGICQKCYGIAPATGKMV
                     AIGDAVGIIAAQSIGEPGTQLTLRTFHTGGVAGLDITQGLPRVVELFEARKPKGLAKL
                     AEVDGKASIERTDKAIKVTVTDDAGEEHAYSFPVRTRLFVADGDTVKAGDQLNEGSIY
                     PHELLDIRGRTDTEVYLVQEVQKVYRSQGVDIKDKHIEIIVRQMMKKVRVDQKGDSDY
                     LPGAFVDRYEMARVNGDLKKAKSEQAQFEEIILGITKASLATDSFLSAASFQETTKVL
                     TDAALEGKTDRLLGLKENVIIGKLIPAATGLKHYRRLEIEPTEPVRRHSPEELGLLDE
                     NDLAAELGLTEDGELEGFGFGQQTQGNGETGAFGDELAELPAEKKDES"
     gene            1618..2076
                     /locus_tag="VFY44_03970"
     CDS             1618..2076
                     /locus_tag="VFY44_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909624.1"
                     /translation="MTRIPTLAIAATAAALVAPGAAAGQEPSYPRCTFSAPKTIKVSA
                     ATRTGVPVRITCNRPSKMLAALDLKFGTRQDDKWALSHNGGIPGISRGRPVQIERTGV
                     AHARLTRAAGRFLNRYPRSAVYVHVAAQNPEHPERYKDVANRRLVTFLGR"
     gene            complement(2081..>2618)
                     /locus_tag="VFY44_03975"
     CDS             complement(2081..>2618)
                     /locus_tag="VFY44_03975"
                     /inference="COORDINATES: protein motif:HMM:NF037735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD domain-containing protein"
                     /protein_id="HEX5909625.1"
                     /translation="ALPKIASRPNTDRKPVLFVDVDGVISLFGFPPGLDDLPGPFHWV
                     DGIAHCIPPHTGPMLERLGERFELVWATGWEEKANEYLPGILGLPFKELPVLTFDGRA
                     VFGSSHWKLDAIDEYAADRPAAWIDDNMDGACELWADHREGPTLLVRTDPPVGLTEEH
                     VELLLFWADEVAAQNEVV"
BASE COUNT          443 a          910 c          917 g          348 t
ORIGIN      
        1 catgcgcggc ctgatgacgg acccgaccgg aaaagtgatc gagctgccga tccgctcctc
       61 gttccgcgag gggctgacgg tgctcgagta cttcatctcg acccacggtg cgcgtaaggg
      121 actggccgac acggctctgc gtacggccga ctccggctac ctgacgcggc gtctggtcga
      181 cgtcgcccag gacgtgatcg tccgcgagga ggattgcggc accaaggagg gcatcgacat
      241 gcccctcttc ggtgaggacg gcaagcccaa cgagaacctg ctcggacgcc acaccagcgt
      301 caagttcggg gacctcaaga agggcgagct gatcgccaag ccgagcctgg ccgcgctggt
      361 cgaggcggcc ggcgacgacg aggccgccac ggttccggtg cgctcggtcc tcaagtgcga
      421 ggccgacacc ggcatctgcc agaagtgtta cggcatcgcg ccggccaccg gcaagatggt
      481 ggccatcggc gacgcggtcg gcatcatcgc cgcgcagtcg atcggcgagc ccggcacgca
      541 gctgacgctg cggacgttcc acaccggcgg cgtcgccggc ctggacatca cgcagggtct
      601 accgcgagtg gtggagctgt tcgaggcacg caagccgaag ggcctggcca agctggccga
      661 ggtcgacggc aaggcgtcca tcgagcgcac cgacaaggcg atcaaggtca cggtgaccga
      721 cgacgcgggc gaggagcacg cgtactcgtt cccggtccgc acgcgcctgt tcgtggccga
      781 cggcgacacc gtcaaggccg gcgaccagct caacgagggg tcgatctacc cgcacgagct
      841 gctggacatc cgcggtcgta ccgacaccga ggtctacctc gtgcaggagg tccagaaggt
      901 ctaccgctcg cagggcgtgg acatcaagga caagcacatc gagatcatcg tgcggcagat
      961 gatgaagaag gtccgcgtgg accagaaggg cgactcggac tacctgccgg gcgccttcgt
     1021 cgaccgctac gagatggctc gcgtcaacgg tgacctcaag aaggcgaagt ccgagcaggc
     1081 ccagttcgag gagatcatcc tcggcatcac caaggcctcg ctggccaccg actcgttcct
     1141 gtcggcggcc tccttccagg agaccaccaa ggtgctcacc gacgccgcgc tcgagggcaa
     1201 gacggaccgc ctcctcgggc tcaaggagaa cgtgatcatc ggcaagctga tccccgccgc
     1261 gaccggtctg aagcactacc ggcgcctgga gatcgagccc accgagcccg tccggcgcca
     1321 ctcgccggag gagctggggc tgctggacga gaacgacctg gccgccgagc tcggcctgac
     1381 cgaggacggc gagctggagg gcttcggctt cggccagcag acccagggca acggcgagac
     1441 cggcgccttc ggcgacgagc tggccgagct gcccgccgag aagaaggacg agtcctaggc
     1501 cccaccgggc cgcagatcgc agcgacgggc cgcctccggg cggcccgtcc cgttcttggg
     1561 ggcaggtgta gggacgtccc tgatggcagg aggggccggc gggcggatgc tgtgtccatg
     1621 acacggatcc ccacactcgc catcgccgcc acggcggccg ccctggtggc ccccggcgcc
     1681 gccgcgggcc aggagccctc gtaccccagg tgcaccttct cggcgccgaa gacgatcaag
     1741 gtgtccgcgg caacccggac cggggtcccg gtgcggatca cctgcaaccg gccctcgaag
     1801 atgctggccg cgctggacct gaagttcggc accaggcagg acgacaagtg ggcgctgtcg
     1861 cacaacggcg ggatccccgg gatctcgcgc gggcggcccg tccagatcga gcgcaccggg
     1921 gtggcccacg cgcggctgac ccgcgccgcc ggccggttcc tcaaccgcta cccgcggtcg
     1981 gcggtctacg tgcacgtggc ggcgcagaac cccgagcacc cggagcgcta caaggacgtg
     2041 gcgaaccgcc ggctggtgac gttcctgggc cgctgagcgg ctagacgacc tcgttctggg
     2101 ccgccacctc gtcggcccag aacagcagca gctccacgtg ttcctcggtc aggcccacgg
     2161 gcgggtcggt gcgcaccagc agcgtcggcc cctcgcggtg gtcggcccac agctcgcagg
     2221 cgccgtccat gttgtcgtcg atccaggccg ccgggcggtc ggcggcgtac tcgtcgatgg
     2281 cgtccagctt ccagtgcgag gagccgaaca ccgcgcgccc gtcgaaggtc aggaccggca
     2341 gctccttgaa gggcaggccc aggatcccgg gcaggtactc gttggccttc tcctcccagc
     2401 cggtggccca gaccagctcg aagcgctccc ccagccgctc cagcatcggg cccgtgtggg
     2461 gcgggatgca gtgggcgatc ccgtcgaccc agtggaaggg ccccggaagg tcgtccagcc
     2521 cgggcgggaa gccgaacagg ctgatcaccc cgtccacgtc cacgaacagc acgggcttgc
     2581 ggtctgtgtt tgggcgagag gcgatcttgg gtagtgcg
//
LOCUS       DASUPW010000174         3105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_179598, whole genome shotgun sequence.
ACCESSION   DASUPW010000174 DASUPW010000000
VERSION     DASUPW010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179598"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1286
                     /locus_tag="VFY44_03980"
     CDS             <1..1286
                     /locus_tag="VFY44_03980"
                     /inference="COORDINATES: protein motif:HMM:NF037020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX5909626.1"
                     /translation="ATGGTTGRIVAIAVAYGVGSGVVLFALAVGGRKLFERVRGVGRG
                     PALQRALGIVLVLTAVAMGTQLDVRFQTTLADHLPQFVVNPTGGIERSAAARERLADV
                     RGEAKFDRATADAKPVSAAGGGRSKYLDLGPAPGFEGGGTWLNTRTPVSLRELRGRVV
                     LIDFWTYTCINCIRTLPQLRTLDARYRRDGLSVVGVHTPEFPFERKTSNVRAAIARNK
                     LRYPVVQDNAYGIWNAYGNQYWPAKYLIDARGRVRYTHFGEGGEKATESAIRALLAES
                     GDRRLGARAKEARAIKPGAGMQTPETYFGAKRAQGWLVPPVAGTRTYRVPGRLPLNGF
                     ALGGRWKVTGESATAVRNATVTARFRARRVYLVLRSTGKRRRDVRVRVDGRTVRTVAV
                     RGDDIYTAVDLPRAGDHDLRLDFAPGVSGYAFTFG"
     gene            1510..3060
                     /locus_tag="VFY44_03985"
     CDS             1510..3060
                     /locus_tag="VFY44_03985"
                     /EC_number="1.2.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF006916.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinic semialdehyde dehydrogenase"
                     /protein_id="HEX5909627.1"
                     /translation="MAETETLATTNGTGDVNGAREQIQVENPATGEVAGSVPRMLPED
                     VAAMVSRARKAQPGWEALGFAGRARVLKRAQAWVINNSDRIIQTIVSETGKTWEDAQL
                     AEVGYAAPAFGFWAKNAEKYLAEEKIRSASPFLLGRKVVLRYRPVGVVGVIGPWNYPL
                     TNSFGDCIPALAAGNAVVLKPSEVTPLTSLLMAECLEESGVPRDVYQVLTGLGDVGAQ
                     LVDEVDFIMFTGSTATGKKVAERAARTLTPVGLELGGKDPMIVLRDADVEKAANAATY
                     YSMQNGGQTCISIERAYVEEPVYDEFVKLVTEKVGALRQGVPTERGAVDVGAVTFEAQ
                     SGIVDSHVRDAVDKGARVLTGGHAGQGPGRYYEPTVLVDVDHSMKCMTEETFGPTLPI
                     MKVRDAEEALRLANDSPYGLQASVWTKDTARGEALARRVESGAVCVNDAQINYAALEL
                     PMGGWKLSGLGSRHGAGGIRKYAKQQTILVTGFAPKRDLHMFPYKAGTTGLLGRAMKL
                     LYGRGKRD"
BASE COUNT          469 a         1104 c         1135 g          397 t
ORIGIN      
        1 gcgccaccgg cggcaccacc gggcggatcg tggccatcgc cgtggcctac ggcgtcggct
       61 ccggcgtggt cctgttcgcc ctggcggtgg gtggccgcaa gctgttcgag cgcgtgaggg
      121 gggtgggccg cgggccggcg ctccagcgcg cgctgggcat cgtcctggtg ctgaccgcgg
      181 tggccatggg aacgcagctg gacgtgcgtt tccagaccac gctggccgat cacctgcccc
      241 agttcgtcgt caaccccacc gggggcatcg agcgctcggc cgcggcccgg gagcgactgg
      301 ccgacgtccg cggcgaggcg aagttcgacc gcgccaccgc cgatgccaag ccggtcagcg
      361 ccgccggcgg cgggaggtcg aagtacctgg acctgggtcc tgctccgggt ttcgagggcg
      421 gcggcacatg gctcaacacg aggacgccgg tctcccttcg cgagctgcgc ggccgggtcg
      481 tgctgatcga cttctggacc tacacctgca tcaactgcat ccgcacgcta ccccagctca
      541 ggacgctgga cgcgcgctac cggagggacg gcctgagcgt ggtgggcgtg cacacgcccg
      601 agttcccctt cgagcgcaag acctccaacg tgcgcgcggc catcgcccgg aacaagctcc
      661 gctacccggt ggtccaggac aacgcgtacg gcatctggaa cgcgtacggc aaccagtact
      721 ggcccgccaa gtacctgatc gacgcgcgtg ggcgcgtgcg ctacacccac ttcggcgagg
      781 gtggcgagaa ggccaccgag tcggccatcc gcgccctgct ggccgagagt ggcgaccgca
      841 ggctgggcgc acgcgccaag gaggccaggg cgatcaagcc cggcgccggc atgcagacgc
      901 ccgagaccta cttcggcgcc aagcgcgccc agggctggct ggtgccgccg gtggcgggca
      961 cgcgcaccta ccgcgtcccc ggccggctgc cgctgaacgg gttcgccctc ggcgggcgct
     1021 ggaaggtcac cggcgagtcg gccaccgccg tccggaacgc caccgtgacc gcccgcttcc
     1081 gcgcgcggcg cgtgtacctg gtgctgcgct cgaccggcaa gcgccgccgt gacgtgcgcg
     1141 tgcgggtcga cggacgcacg gtccggaccg tggccgtgcg cggcgacgac atctacaccg
     1201 cggtcgacct gcccagggcg ggggaccacg acctccggct ggacttcgcg ccgggcgtca
     1261 gcggatacgc gttcacgttc gggtgacgca cccgccgtca agggtgagtc actacttccg
     1321 tctctccact cgacgcttct gcgaactcat caggcaccgg gtcacgaacg agcgaacggt
     1381 caccgcccgg gcgcctaccc agccaagcct tccgccgacc ccgcagggga cgtaacacgg
     1441 agaacgaaac tggccagccg gccagtcccc gttatgctcc ccgggcacgc gcgcgaccgg
     1501 agggtgagaa tggctgagac ggagacgctg gcgaccacga acggcacggg ggacgtcaac
     1561 ggcgcccgcg agcagatcca ggtcgagaac ccggcgacgg gcgaggtggc cggctcggtg
     1621 ccgcgcatgc tgcccgagga cgtggccgcg atggtctcgc gcgcccgcaa ggcacagccg
     1681 ggctgggaag cgctgggctt cgccggccgc gcccgggtgc tcaagcgcgc gcaggcgtgg
     1741 gtgatcaaca actccgaccg gatcatccag acgatcgtgt ccgagaccgg gaagacctgg
     1801 gaggacgcgc agctggccga ggtgggctac gcggcccccg cgttcggctt ctgggccaag
     1861 aacgccgaga agtacctggc cgaggagaag atccgctccg cctcgccgtt cctgctgggc
     1921 cgcaaggtcg tgctgcgcta ccgccccgtc ggcgtggtgg gcgtgatcgg cccctggaac
     1981 tacccgctga ccaacagctt cggcgactgc atcccggcgc tggccgccgg caacgcggtg
     2041 gtgctcaagc cgtcggaggt cacgccgctg acgtcgctgc tcatggccga gtgcctggag
     2101 gagtcgggcg tcccgcgcga cgtctaccag gtgctcacgg gcctgggtga cgtgggcgcc
     2161 cagctggtcg acgaggtcga cttcatcatg ttcaccggct ccaccgcgac cggcaagaag
     2221 gtcgccgagc gcgcggcccg gacgctgacc ccggtcggcc tggagctggg cggcaaggac
     2281 ccgatgatcg tgctgcgcga cgccgacgtg gagaaggcgg ccaacgcggc cacctactac
     2341 tcgatgcaga acggcggcca gacgtgcatc tcgatcgagc gcgcctacgt cgaggagccg
     2401 gtctacgacg agttcgtgaa gctggtcacc gagaaggtgg gggcgctgcg tcagggcgtg
     2461 cccaccgagc gcggcgcggt ggacgtgggg gcggtcacgt tcgaggccca gtcgggcatc
     2521 gtggactccc acgtgcgcga cgccgtggac aagggcgcgc gcgtgctcac cggcggccac
     2581 gccggccagg gtcccgggcg ctactacgag cccacggtcc tggtcgacgt ggaccacagc
     2641 atgaagtgca tgaccgagga gaccttcggc ccgacgctcc cgatcatgaa ggtccgcgac
     2701 gccgaggagg cgctgcggct cgccaacgac tcgccgtacg gcctgcaggc ctcggtgtgg
     2761 acgaaggaca cggcccgcgg cgaggcgctc gcgcgccgcg tcgagtccgg cgcggtgtgc
     2821 gtgaacgacg cgcagatcaa ctacgccgcg ctcgagctgc cgatgggcgg ctggaagctg
     2881 tcgggtctgg gctcgcgcca cggcgccggc ggcatccgca agtacgccaa gcagcagacg
     2941 atcctcgtca ccgggttcgc gcccaagcgc gacctgcaca tgttcccgta caaggccggc
     3001 acgaccggcc tgctcggccg cgcgatgaag ctgctctacg gccgcgggaa gcgcgactga
     3061 cgagcgcgcc gccggcgcgc cgcgagggcg ccccgccggc gcggc
//
LOCUS       DASUPW010000175         6722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1426923, whole genome shotgun sequence.
ACCESSION   DASUPW010000175 DASUPW010000000
VERSION     DASUPW010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6722
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426923"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VFY44_03990"
     CDS             <1..879
                     /locus_tag="VFY44_03990"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX5909628.1"
                     /translation="LRRMPVAIVADSTAYLPPERLERHSITVVPLYVVFGSDRTVRED
                     LITDYPAYFEELRSAEALPGTSQPSVGDFVAAYEPLLEQGYDIASVHISEGLSGTGQS
                     GRQAAEQLAREGKGGERIRILDSRTAAGGTGLMVLAAAKAAEDGGDLDAVEANMEAAR
                     ANFKMWFAVDTLEFLRRGGRIGAANAWIGSTLKIKPILTVESEMVPVERVRTAKRSFE
                     RMVDYARQRHESGADGWVVQHISVPDQAEELIRRCTEIFECDPTFVSEVGPVLSTHTG
                     PGMLGVGSVPRDLVPA"
     gene            926..2050
                     /locus_tag="VFY44_03995"
     CDS             926..2050
                     /locus_tag="VFY44_03995"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5909629.1"
                     /translation="MQPRDSLSRVVLRTVLIVVAVVFALYILYLVRKPISWLLIATFL
                     AVALTGPVNLLQRHMKRGFAITLVFLGLLLTPVAIGALVLPPIVNGADDLARNAPAYA
                     NDIADFVRDDPRLKEVNEDYDITSKLEEEAAQLPARLGDAATVLRDVGFGIVNSIFAV
                     VTILIMTAFILGGGRRWIEHGLRYLPVERAERVERVLQRSARAVGSYVAGVLFQAFTA
                     GILAYIVLSILGVPFAAPLAVTIFFLDLIPMVGATIGAVIVALVTVFNDFPTDTIIWV
                     IWAIVYQQIENNVVQPQIQKRAVQINPFLVIVSVLFGGTLLGILGAVIAVPVAASIQI
                     AIREYMDERGIKPRDQIGPAEEPPPEPPGEAPGGPEPAPA"
     gene            complement(2047..2289)
                     /locus_tag="VFY44_04000"
     CDS             complement(2047..2289)
                     /locus_tag="VFY44_04000"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEX5909630.1"
                     /translation="MSDLPANRTPEQIRSNIETTRQELAFSVNDLRSKVTEITNWRKQ
                     LADNREAALATAAVAGFVIGGGVAATVSLFRRRRGR"
     gene            complement(2286..2750)
                     /locus_tag="VFY44_04005"
     CDS             complement(2286..2750)
                     /locus_tag="VFY44_04005"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX5909631.1"
                     /translation="MSAPPPNSDKSVGEIVSEVSEKASLLVREEIALAKAEVAGKVKT
                     LGKGAGVAAAAGVFLFFAFIFFLEALAWFFNDLLNVVTAIWLGFLIVFLILVALAVIA
                     GLLAKKWLSSGPPTPDMAIEEAKATRDQFEHQTIERDQVTRSLERDKETTEP"
     gene            complement(2747..2947)
                     /locus_tag="VFY44_04010"
     CDS             complement(2747..2947)
                     /locus_tag="VFY44_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909632.1"
                     /translation="MEASDSSNVSAAPWATGDQPKQQSPADAHTAAAEADAFAERPEV
                     YVGAAFAGGLALAVGLRWLGSR"
     gene            complement(2947..3084)
                     /locus_tag="VFY44_04015"
     CDS             complement(2947..3084)
                     /locus_tag="VFY44_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909633.1"
                     /translation="MDNELVKRLVWSGMLAGVGALSTIVANKIATALWIRLFNEDPPD
                     Y"
     gene            complement(3095..3736)
                     /locus_tag="VFY44_04020"
     CDS             complement(3095..3736)
                     /locus_tag="VFY44_04020"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5909634.1"
                     /translation="MAARPGNGGGVDKRRLILDAAITVFARQGFYNCRVSDVADEAGV
                     AYGLVYHYFDSKEEILNELFLERWQIMLDAIAEIDARDDLAVRDKLQLVASFIIDSYS
                     NEPDLMKVIIVEVTRAANSFGRIHLDKIREAYRGIAEIVEAGRADGDFKQDISAEFAA
                     MCFYGAIEQLLSGWIFDLLPPTEDEFERAKGQVVEAICDGLATPVADGRRVSG"
     gene            complement(3736..4563)
                     /locus_tag="VFY44_04025"
     CDS             complement(3736..4563)
                     /locus_tag="VFY44_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX5909635.1"
                     /translation="MRAAAVQLNSTDDKAANMASADRLTRAAAADGADLVVLPEKVNL
                     LGPDAALRDGAETLEGATVAWARETAEELGIDLVAGSFAERREGHDKLSNTSVHVGPD
                     GQVRGVYRKIHMFDVTVEGKEYRESAAQERGEEIVLSDAEGARLGLTVCYDLRFPELY
                     RILTLRGARVLVVPSAFTRATGTAHWDTLVRARAIENQAFVVAADQIGIHPPDNESFG
                     GSQIVDPWGDVLARAGDEVGFVAADLDFERQDEVRATLPSLANRVPSAYRWEQEVGV"
     gene            complement(4622..6037)
                     /locus_tag="VFY44_04030"
     CDS             complement(4622..6037)
                     /locus_tag="VFY44_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5909636.1"
                     /translation="MSSTVPAALTHNPFLRIAQDIRHEVAERGNFPPGELGFSARRTH
                     QLATDPLPVLLSMYERYGPVFTVRTLHVPQVFALGPEANHYITVSHAANFRWRDGGFA
                     ELEPLLGDGLLTIDGPYHRRARKVMLPGFHRTQIAAAADVMVDEIDAALGHWRPDTSF
                     DLYTWARTLAMRIAMKALMGLDPDGDGVGARAAEEFEKALHFYGTDYHVRVVRGPGSP
                     WAKMLRARGELDRIVYGEIDRRRRAGVGQDDILDMLLAATDDEGNPLTDREVRDQAIT
                     LLFAGHDTTTSTISFLFYELARHPHEVALLLDEQQRVLEGGRAPAAKDLAGGMPRLEM
                     VLDEVLRLYPPAWIGPRRAVEAFELAGAHVPAGAPVNYSSWASHRLPDVWPDPEAFLP
                     ERFAPEAKAALPKGAYVPFGGGSRTCIGMRFGEMEIRAIATMVLQRFRLELVPGRTMT
                     IRQMPTLSPRHGMPVTVRARS"
     gene            6098..>6722
                     /locus_tag="VFY44_04035"
     CDS             6098..>6722
                     /locus_tag="VFY44_04035"
                     /inference="COORDINATES: protein motif:HMM:NF022427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HEX5909637.1"
                     /translation="MLAAPVRGPAVRNLGLPLPPQAMPGWRRGRPLKSWRYLGVYTPE
                     LMLCVGDARIGPARQRWWALAWPDGRLRERTTIRRGGVTMDPARVRVHAGDVRIDLAL
                     DEPAAVETISPADGGYVWTAKRAPIGVTGMVTVGPNRHHIDGPFGFVDDSAGYHDRHT
                     VWRWSAGVGTASDGRPVAWNLVDGIHDDARASERTLWVDGAPQEIGPN"
BASE COUNT          938 a         2411 c         2375 g          998 t
ORIGIN      
        1 ctccggcgaa tgcccgtcgc catcgtcgcc gacagcacgg cgtacctccc ccccgagcgc
       61 ctcgagcgtc actccatcac cgtggtcccg ctgtacgtgg tcttcggctc cgaccgcacc
      121 gtgcgcgagg acctgatcac cgactacccg gcctacttcg aggagctgcg cagcgccgag
      181 gcgctgccgg ggacctcgca gccgtcggtc ggcgacttcg tggccgcgta cgagccgctc
      241 ctggagcagg gctacgacat cgcctcggtg cacatctccg aggggctctc gggcacgggc
      301 cagtccgggc gccaggcggc cgagcagctg gcccgcgagg gcaagggcgg cgagcggatc
      361 cggatcctgg actcgcgcac cgcggcgggc gggaccgggc tgatggtcct ggccgcggcc
      421 aaggccgccg aggacggcgg cgacctggat gcggtcgagg ccaacatgga ggcggccagg
      481 gccaacttca agatgtggtt cgccgtcgac acgctcgagt tcctgcgccg cggcgggcgg
      541 atcggcgcgg ccaacgcgtg gatcggctcg acgctgaaga tcaagccgat cctgaccgtg
      601 gagagcgaga tggtcccggt cgagcgggtg cgcaccgcga agcgctcgtt cgagcggatg
      661 gtcgactacg cccgccagcg ccacgagtcc ggcgccgacg gctgggtcgt ccagcacatc
      721 tcggtgcccg accaggccga ggagctgatc cggcgctgca ccgagatctt cgagtgcgac
      781 ccgaccttcg tcagcgaggt cggtccggtg ctgtccaccc acaccgggcc gggcatgctg
      841 ggcgtgggct cggtcccgcg cgatctggtc cccgcctagg cctctcgccc cgggagcgtc
      901 cgggcgtgcc cgcacaatcg ggcccgtgca gccacgcgac tccctctcca gggtggtcct
      961 ccggaccgtc ctgatcgtcg tcgcagtcgt cttcgcgctc tacatcctct acctggtgcg
     1021 caagccgatc agctggctgc tgatcgccac gttcctggcg gtcgccctga ccggccccgt
     1081 gaacctgctc cagcggcaca tgaagcgcgg gttcgccatc acgctggtgt tcctgggcct
     1141 gctgctcacg ccggtcgcga tcggcgccct ggtgctgccg ccgatcgtca acggcgccga
     1201 cgacctggcc cgcaacgcgc cggcctacgc caacgacatc gccgacttcg tccgcgacga
     1261 cccgcggctg aaggaggtca acgaggacta cgacatcacg tcgaagctcg aggaggaggc
     1321 ggcccagctg cccgcgcgcc tcggcgacgc cgccaccgtg ctgcgcgacg tgggcttcgg
     1381 gatcgtgaac tcgatcttcg ccgtggtgac gatcctgatc atgaccgcgt tcatcctcgg
     1441 cggcggcaga cgctggatcg agcacgggct gcggtacctg cccgtggaac gcgcggaacg
     1501 ggtcgagcgg gtgctccagc gctccgcgcg agcggtcggc agctacgtgg ccggggtgct
     1561 gttccaggcc ttcacggccg ggatcctggc ctacatcgtg ctgtcgatcc tgggcgtgcc
     1621 gttcgcggcc cccctggccg tgaccatctt cttcctggac ctgatcccca tggtcggcgc
     1681 caccatcggc gcggtgatcg tggccctggt caccgtgttc aacgacttcc cgaccgacac
     1741 gatcatctgg gtcatctggg cgatcgtcta ccagcagatc gagaacaacg tggtccagcc
     1801 ccagatccag aaacgcgcgg tccagatcaa cccgttcctg gtcatcgtct ccgtgctgtt
     1861 cggtggcacg ttgctgggca tcctgggggc ggtcatcgcc gtgcccgtgg cggcgtcgat
     1921 ccagatcgcc atccgcgagt acatggacga gcgtggcatc aagccgcgcg accagatcgg
     1981 gccggccgag gaaccgccgc ccgagccccc cggtgaagcg cccggcgggc cggagcccgc
     2041 gccggcctag cgcccgcggc gcctgcggaa cagcgagacc gtcgcggcga cgccgccgcc
     2101 gatcacgaaa ccggcaaccg ccgcggtggc cagcgcggcc tcgcggttgt cggccagctg
     2161 cttgcgccag ttggtgatct cggtgacctt ggaacgcagg tcgttgaccg agaacgccag
     2221 ctcctgacgc gtggtctcga tgttcgagcg aatctgctcc ggagtgcggt ttgcgggcag
     2281 gtcgctcatg gctcggtcgt ctccttgtcg cgctccaggg aacgggtcac ctgatcgcgc
     2341 tcgatggtct ggtgctcgaa ctggtcacgg gtggccttgg cttcctcgat cgccatgtcc
     2401 ggcgtgggtg gcccgctgga gagccatttc ttggccagca gcccggcgat cacggccagc
     2461 gcgaccaaga tcaggaagac gatcaggaag cccagccaga tcgcggtgac cacgttcagg
     2521 aggtcgttga agaaccaggc cagcgcctcg aggaagaaga tgaaggcgaa gaacaggaac
     2581 acgccggcgg cggcggcgac gccggcgccc ttgccgagcg tcttgacctt gccggcgacc
     2641 tcggccttgg ccagcgcgat ctcctcgcgc accagcagcg aggccttctc ggagacctcc
     2701 gagacgatct cgccgacgga cttgtcggag ttgggcgggg gcgcgctcat cggctgccca
     2761 gccatctgag cccgacggcc agggccaggc cgcccgcgaa ggcggcgccc acgtagacct
     2821 ccggccgctc ggcgaaggcg tcggcctcgg cggccgcggt gtgcgcgtcg gccggggact
     2881 gctgcttggg ctggtcgccc gtcgcccagg gggcggcgga cacgttgctg ctgtccgagg
     2941 cttccatcag tagtcgggcg ggtcctcgtt gaacaggcgg atccacaggg ccgtggcgat
     3001 cttgttggcc acgatcgtgc tcagggcgcc cacaccggcg agcatgccgc tccagacgag
     3061 gcgcttgacg agttcgttgt ccattggcgg tgattcaacc agacacgcgg cggccgtcgg
     3121 caaccggggt ggccaggccg tcgcatatgg cttcgactac ctggcccttg gcccgctcaa
     3181 actcgtcctc ggtcggcggc agcagatcga agatccagcc cgacagcagc tgctcgatgg
     3241 cgccgtagaa gcacatggcg gcgaactcgg ccgagatgtc ctgcttgaaa tcgccgtcgg
     3301 cccggcccgc ctcgacgatc tcggcgatgc cgcggtaggc ctcgcggatc ttgtccaggt
     3361 ggatgcgccc gaacgagttc gcggcgcgcg tgacctcgac gatgatcacc ttcatcaggt
     3421 ccggctcgtt gctgtacgag tcgatgatga agctggccac cagctgcagc ttgtcgcgca
     3481 ccgccaggtc gtcgcgagcg tcgatctcgg cgatcgcgtc cagcatgatc tgccaccgct
     3541 ccaggaagag ctcgttgagg atctcctcct tcgagtcgaa gtagtggtag accagcccgt
     3601 aggccacgcc ggcctcgtcg gccacgtccg agacgcggca gttgtagaag ccctggcgcg
     3661 cgaacaccgt gatggccgcg tccaggatca ggcggcgctt gtccacccct ccgccgttgc
     3721 cgggccgcgc cgccatcaga cccccacctc ctgctcccac cggtacgccg agggcacgcg
     3781 gttggccagg ctgggcagcg tggcgcggac ctcgtcctgg cgctcgaagt ccaggtcggc
     3841 ggccacgaag cccacctcgt cgccggccct ggccagcacg tcgccccagg ggtcgacgat
     3901 ctgcgagccg ccgaagctct cgttgtcggg cggatggatg ccgatctggt cggcggccac
     3961 cacgaaggcc tggttctcga tcgcccgcgc ccgcaccagc gtgtcccagt gggcggtccc
     4021 ggtggcgcgg gtgaacgccg acggcaccac cagcacgcgc gcgccgcgca gggtcaggat
     4081 ccggtagagc tcggggaagc gcaggtcgta gcacacggtc aggcccagcc gggcgccctc
     4141 ggcgtcggag agcacgatct cctcgccccg ctcctgggcc gcggactcgc ggtactcctt
     4201 gccctccacg gtcacgtcga acatgtggat cttgcggtag acgccgcgca cctggccgtc
     4261 ggggcccacg tggaccgagg tgttggagag cttgtcgtgg ccctcacggc gctcggcgaa
     4321 cgagccggcc accaggtcga tgcccagctc ctccgcggtc tcgcgcgccc acgccacggt
     4381 cgcgccctcg agcgtctcgg cgccgtcgcg cagcgccgcg tcggggccca gcaggttgac
     4441 cttctccggc agcacgacca ggtcggcgcc gtcggcggcc gcggcacggg tgagccggtc
     4501 ggcggacgcc atgttggccg ccttgtcgtc cgtcgagttg agctgcactg ctgccgcgcg
     4561 catgaccctc cccggtgctc gcgattgact ggtcagtcaa cgagtctagg gcttcggagc
     4621 gtcaggaacg cgcccgcacg gtcacgggca tgccgtggcg cgggctgagg gtgggcatct
     4681 ggcggatggt catcgtgcgg cccggcacca gctccagccg gaagcgctgg agcaccatcg
     4741 tcgcgatggc gcggatctcc atctcgccga agcgcatgcc gatgcacgtg cgcgagccgc
     4801 ccccgaacgg cacgtaggcg cccttgggca gggcggcctt ggcctcgggc gcgaaccgct
     4861 cgggcaggaa cgcctcggga tccggccaga cgtcgggcag ccggtggctg gcccaggagc
     4921 tgtagttcac cggggcgccg gcgggcacgt gggcgcccgc cagctcgaac gcctcgacgg
     4981 cgcgccgcgg cccgatccag gccggcgggt acagccgcag gacctcgtca agcaccatct
     5041 ccaggcgcgg catgccgccg gccaggtcct tcgcggccgg cgcccggccg ccctccagga
     5101 cacgctgctg ctcatccagc agcagcgcca cctcgtgcgg gtgccgcgcc agctcgtaga
     5161 acaggaagct gatcgtcgag gtggtggtgt cgtggcccgc gaacagcagc gtgatggcct
     5221 ggtcgcgcac ctcgcggtcg gtgagcgggt tgccctcgtc gtcggtggcc gccagcagca
     5281 tgtccaggat gtcgtcctga cccacgccgg cgcggcggcg gcggtcgatc tcgccgtaga
     5341 cgatccgatc gagctcgccc cgggcacgca gcatcttcgc ccacggcgag cccggtccgc
     5401 gcaccacccg cacgtggtag tcggtgccgt agaagtgcag cgccttctcg aactcctccg
     5461 ccgcccgtgc ccccacgccg tcgccgtcgg gatccaggcc catcagcgcc ttcatggcga
     5521 tccgcatcgc cagcgtgcgc gcccacgtgt agaggtcgaa ggacgtgtcc ggccgccagt
     5581 ggccgagcgc ggcgtcgatc tcgtcgacca tcacgtccgc cgcggcggcg atctgggtcc
     5641 ggtggaagcc cggcagcatg accttgcgcg cgcgccggtg gtaggggccg tcgatggtca
     5701 gcaggccatc gcccagcagc ggctcgagct cggcgaagcc gccgtcgcgc cagcggaagt
     5761 tggccgcgtg cgagacggtg atgtagtggt tggcctcggg gccgagcgcg aacacctgcg
     5821 gcacgtgcag cgtgcgcacg gtgaacaccg gcccgtagcg ctcgtacatc gacagcagca
     5881 ccggcaacgg gtcggtggcc agctggtgtg tgcggcgggc gctgaagccc agctcgccgg
     5941 gcgggaagtt gccgcgctcg gcgacctcgt ggcggatgtc ctgcgcgatc cgcaggaacg
     6001 ggttgtgcgt cagtgcggcg gggaccgtgg acgacacggc gcgaaccata ccgggctggc
     6061 cagccagcca gttttgccgg ggctatagcc tgcgcccgtg ctcgcagcac ccgtacgtgg
     6121 ccccgccgtg cgcaacctcg gcctgcccct cccgccccag gcgatgccgg gctggcgtcg
     6181 cgggcggccg ctcaagtcct ggcgctacct gggcgtctac acccccgagc tgatgctgtg
     6241 cgtgggcgac gcgcggatcg ggccggcgcg ccagcgctgg tgggcgctgg cgtggcccga
     6301 cggccgcctg cgcgagcgga ccacgatccg gcgcggcggc gtgaccatgg accccgcgcg
     6361 cgtgcgggtg cacgccggcg acgtgcggat cgacctcgcg ctcgacgagc ccgccgccgt
     6421 cgagaccatc tccccggccg acggcggcta cgtgtggacg gccaagcggg ccccgatcgg
     6481 cgtgaccggg atggtcaccg tgggcccgaa ccgccatcac atcgacggcc cgttcggctt
     6541 cgtcgacgac tcggccggct accacgaccg gcacacggtg tggcgctgga gcgcgggcgt
     6601 cggcaccgcc tccgacggcc ggccggtggc ctggaacctc gtggacggca tccacgacga
     6661 cgcccgtgcc agcgagcgca cgctgtgggt ggacggcgcg ccgcaggaga tcggccccaa
     6721 cc
//
LOCUS       DASUPW010000176         2080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_873201, whole genome shotgun sequence.
ACCESSION   DASUPW010000176 DASUPW010000000
VERSION     DASUPW010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873201"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /gene="pgk"
                     /locus_tag="VFY44_04040"
     CDS             <1..403
                     /gene="pgk"
                     /locus_tag="VFY44_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX5909638.1"
                     /translation="KLQLPVDVVAGRAFDADTETQAIDGLDVPDGWMGLDVGERTAAR
                     YAQVIAGAGTVFWNGPMGAFELAPFEAGTRAVAQAVADCPGTTVVGGGDSAAAIAEFG
                     LADRVTHLSTGGGAALELLEGKTLPGVEVLS"
     gene            404..1159
                     /gene="tpiA"
                     /locus_tag="VFY44_04045"
     CDS             404..1159
                     /gene="tpiA"
                     /locus_tag="VFY44_04045"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX5909639.1"
                     /translation="MPDRKPYIAGNWKLWGTRAQAADYCKAFAPLLDDPAADVGLFVP
                     FTALDTVVSGLEGSGVAVGAQTMSAEDSGAFTGEVSAAMLTEAGATAVVLGHSERRQY
                     YGETDRALQAKVPAALEAGLAPILCVGETEEEREAGDMERKLRHQIGEGLEAVPEERL
                     ADVTVAYEPIWAIGTGQVATPDQAQEAIGFVRALVGGRSEEAAQRIRVLYGGSVKPGN
                     AAEILSQPDVDGALVGGASLDPEGFAQIVAAAG"
     gene            1156..>2080
                     /gene="gpmI"
                     /locus_tag="VFY44_04050"
     CDS             1156..>2080
                     /gene="gpmI"
                     /locus_tag="VFY44_04050"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX5909640.1"
                     /translation="MSQTFPAVTLIVLDGWGLAEPGPGNAVELADTPVFDELWEKHPH
                     TQLTAWGPAVGLPEGQMGNSEVGHLNLGAGQIVAQDLKRIDDAVADGSFAENEVLRDA
                     CAAARESGRLHLLGLVSEGGVHASMDHLKALLELAADQGVPDVVVHAFTDGRDTGPKS
                     GAGYLETVQGWLDATGARIADVTGRYFAMDRDKREERIAKAMDAIVRGEAEGRHADSA
                     VRAVEDAYERDETDEFVEPTLVGEEGTVRDGDACVFFNFRPDRARELTEALNAELDLG
                     YFATLTQYQEDWDYPVAFPPERPEVTLASTLA"
BASE COUNT          291 a          746 c          788 g          255 t
ORIGIN      
        1 caagctccag ctgccagtcg acgtggtggc cggcagggcg ttcgacgccg acaccgagac
       61 ccaggccatc gatggcctgg acgtgcccga cggctggatg ggcctggacg tcggcgagcg
      121 caccgccgca cgctacgccc aggtcatcgc cggcgccggc accgtgttct ggaacggccc
      181 catgggggcc ttcgagctgg ccccgttcga ggccggcacc cgtgccgtgg cgcaggccgt
      241 ggccgactgc cccggcacca cggtggtggg cgggggcgac tccgccgccg ccatcgccga
      301 gttcggcctg gccgaccggg taacccatct gtccacgggc gggggcgccg ccctcgagct
      361 cctggagggc aagacactcc cgggagtgga ggttctttcg tagatgccag accgcaagcc
      421 ctacatcgcc ggcaactgga agctctgggg caccagggcc caggccgccg actactgcaa
      481 ggcgttcgcg ccgctgctgg acgaccccgc ggccgacgtg gggctcttcg tgcccttcac
      541 cgccctggac accgtggtgt ccggcctgga gggcagcggc gtggcggtcg gcgcccagac
      601 catgtccgcc gaggactcgg gcgcgttcac cggcgaggtg tccgcggcca tgctcaccga
      661 ggccggcgcc accgccgtgg tcctgggcca ctccgagcgg cgccagtact acggcgagac
      721 cgaccgcgcg ctgcaggcca aggtcccggc cgcgctggag gcgggcctgg ccccgatcct
      781 gtgcgtgggc gagaccgagg aggagcgcga ggccggcgac atggagcgca agctgcgcca
      841 ccagatcggc gagggcctgg aggccgtgcc cgaggagcgg ctggccgacg tgaccgtggc
      901 ctacgagccc atctgggcca tcggcaccgg ccaggtggcc acgcccgacc aggcccagga
      961 ggccatcggc ttcgtgcgcg cgctcgtggg cgggcgctcc gaggaggccg cccagcgcat
     1021 ccgcgtcctg tacggcggat cggtcaagcc cggcaacgcc gccgagatcc tgtcccagcc
     1081 cgacgtggac ggcgcgctgg tcggaggggc gtcgctggac cccgagggct tcgcgcagat
     1141 cgtcgccgcc gcgggatgag ccagaccttc ccggccgtca ccctgatcgt cctcgacggc
     1201 tggggcctgg ccgagccggg accgggcaac gccgtggagc tggccgacac gccggtgttc
     1261 gacgagctgt gggagaagca cccgcacacc cagctgaccg cgtggggccc ggcggtgggc
     1321 ctgcccgagg gccagatggg caactccgag gtgggtcacc tgaacctggg cgccggccag
     1381 atcgtggccc aggacctcaa gcgcatcgac gacgcggtgg cggacggctc gttcgccgag
     1441 aacgaggtgc tgcgcgacgc ctgcgccgcc gcccgcgagt cgggccggct gcacctgctg
     1501 ggcctggtct ccgagggcgg cgtgcacgcg tcgatggacc acctgaaggc gctgctggag
     1561 ctggccgccg accagggcgt gcccgacgtg gtggtgcacg ccttcaccga cgggcgcgac
     1621 accggtccca agtcgggcgc cggctacctg gagacggtgc agggctggct ggatgcgacc
     1681 ggcgcgcgca tcgccgacgt gacgggccgc tacttcgcga tggaccgcga caagcgcgag
     1741 gagcggatcg cgaaggccat ggacgccatc gtccgcggcg aggccgaggg gcgccacgcc
     1801 gacagcgcgg tccgggccgt ggaggacgcg tacgagcgcg acgagaccga tgagttcgtc
     1861 gagcccaccc tggtgggtga ggagggcacc gtccgcgacg gcgacgcctg cgtgttcttc
     1921 aacttccgtc ccgaccgcgc gcgcgagctg acagaggcgc tgaacgccga gctggacctg
     1981 ggctacttcg ccaccctgac gcagtaccag gaggactggg actacccggt ggccttcccg
     2041 cccgagcggc ccgaggtcac gctggcgtcg acgctggccg
//
LOCUS       DASUPW010000177         3895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1427279, whole genome shotgun sequence.
ACCESSION   DASUPW010000177 DASUPW010000000
VERSION     DASUPW010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427279"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VFY44_04055"
     CDS             <1..808
                     /locus_tag="VFY44_04055"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX5909641.1"
                     /translation="TAEIKERLSDELDYEQEAQAQRAFARAFRGHPFVVIPDVVTSLS
                     REKVLVTEWVEGIGFEKVRELPEDERNRYGEMIFRFFFGSMHRNAHFSGDPHPGNFRF
                     MPDGRIAFLDFGMTKVVHREHIENEAQALRLGMEGDAQGLFDHLAGMGFFDPEDPVMT
                     PEKVLEHFRAVTSWYVEDREVTIDKDVVAQVLIDFGDPRSDYWEMLRHENLPPEAILG
                     RRMEGLTVGVLGALGCTANWHRIWREWLYGDPPTTPLGEEEAEFYGLAAA"
     gene            808..1440
                     /locus_tag="VFY44_04060"
     CDS             808..1440
                     /locus_tag="VFY44_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEX5909642.1"
                     /translation="MPLPFSTEAMEHLMAADPVMEAIVGRVGPLDPEARTRGRPDDSY
                     GALVRSITGQQLSVKAARTIYLRLADLYGGRTPTPAELIATDPDDLRAVGLSYRKAAY
                     LRDLAERIESGELAPERFPELEDREVSEMLTAVKGVGQWTADVFMMFHLGREDVLPVG
                     DLGIRKAAEIEYGLRKLPSPDRLRRLARPWRPYRTLACVYLWESLDNAPD"
     gene            1528..1761
                     /locus_tag="VFY44_04065"
     CDS             1528..1761
                     /locus_tag="VFY44_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909643.1"
                     /translation="MSEMIGSAEKPPDGVPSNGIIVLNDLEQGRSLVIGLFETEADLK
                     TGDEALRAMDSPPEAAGDVSSVEFYEVAVDRRM"
     gene            complement(1766..2512)
                     /locus_tag="VFY44_04070"
     CDS             complement(1766..2512)
                     /locus_tag="VFY44_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909644.1"
                     /translation="MRPSSAWLSSAALLTLAAAAAPATAAGATVDLSGTAYEFNKPAT
                     RLAGATVRVAERPRLRATVRKNGAYRLAVPDRARVTPYVDAPGYHRIYLQTFTTAGKD
                     LTRVNFQVPTDAVYNALATLLKVPLDAKGNIRACAIVSTFSTRKVRNLSFNGFVRYGA
                     HGVAGATASATPKLKSPFYFNEQVIPDPAQRRSSKDGGVIWTGVRSGRYVVRGHHPRT
                     RFASFVATCKPGRIVNANPPWGLHELKPAR"
     gene            complement(2484..2912)
                     /locus_tag="VFY44_04075"
     CDS             complement(2484..2912)
                     /locus_tag="VFY44_04075"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5909645.1"
                     /translation="MAKPGDELLNPHTGQTITFVSIEDDLLVMESSYKAGGAQAPAHL
                     HPSQEERFEVLSGAVLTAVDGEQQMLVEGDRITIPAGTPHTFGGHPEQDGTVRWEVRP
                     ALRTDELFELLFGLADGSRTMPDDGSNPLEEYAPEFRLAV"
     gene            complement(2923..3264)
                     /gene="erpA"
                     /locus_tag="VFY44_04080"
     CDS             complement(2923..3264)
                     /gene="erpA"
                     /locus_tag="VFY44_04080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX5909646.1"
                     /translation="MTEIAADKVRGFLAQQETEGEVGLRIGVRGGGCSGFQYALALDE
                     KKEEDQVFDHEGIRLIVDPASMRYVDGSQVDYTESLMGAGFEVKNPNVVASCGCGSSF
                     RIADEDPSCGS"
     gene            3344..3571
                     /locus_tag="VFY44_04085"
     CDS             3344..3571
                     /locus_tag="VFY44_04085"
                     /inference="COORDINATES: protein motif:HMM:NF013851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HEX5909647.1"
                     /translation="MPTAEHLKERLVEAFPDADVDVSGDDGQHFRAEIVSARFEGLTR
                     IQQHKLVQEVFGGELGGAIHALSIKTSTPEG"
     gene            3574..>3895
                     /gene="grxD"
                     /locus_tag="VFY44_04090"
     CDS             3574..>3895
                     /gene="grxD"
                     /locus_tag="VFY44_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEX5909648.1"
                     /translation="MDNAEIRDFIDSSIKENEVMLFMKGTPEAPACGFSMRTSGALNA
                     LGVRYAALDILPDPRIREELSGLSGGPTIPQLFVDGELVGGSDIVLEMFESGELAEVL
                     GVEQP"
BASE COUNT          590 a         1311 c         1404 g          590 t
ORIGIN      
        1 gaccgccgag atcaaggagc gcctgtccga cgagctggac tacgagcagg aggcccaggc
       61 ccagcgcgcg ttcgcgcgcg ccttccgcgg ccatccgttc gtggtgatcc ccgacgtggt
      121 gacctcgctg tcgcgcgaga aggtgctggt gaccgagtgg gtcgagggca tcggcttcga
      181 gaaggtgcgc gagctgcccg aggacgaacg caaccgctac ggcgagatga tcttccgctt
      241 cttcttcggc tccatgcacc gcaacgcgca cttctcgggc gacccccacc cgggcaactt
      301 ccgcttcatg cccgacggcc ggatcgcgtt cctggacttc ggcatgacca aggtcgtgca
      361 ccgcgagcac atcgagaacg aggcccaggc gctgcgcctg ggcatggagg gcgacgccca
      421 gggcctgttc gaccacctgg cgggcatggg cttcttcgat cccgaggacc cggtcatgac
      481 gcccgagaag gtgctggagc acttccgggc cgtgaccagc tggtacgtgg aggaccgcga
      541 ggtgaccatc gacaaggacg tggtggccca ggtgctgatc gacttcggcg acccgcgctc
      601 ggactactgg gagatgctcc gccacgagaa cctcccgccc gaggccatcc tgggccggcg
      661 gatggagggc ctgaccgtgg gcgtcctggg cgcgctgggc tgcaccgcca actggcaccg
      721 gatctggcgc gagtggctct acggcgaccc gcccaccacg ccgctgggtg aggaagaggc
      781 cgagttctac ggcctcgccg cggcgtagtg ccgctgccct tctcgaccga ggcgatggaa
      841 cacctgatgg ccgccgaccc ggtcatggag gcgatcgtgg ggcgggtggg gccgctggac
      901 ccggaggcgc gcacgcgcgg gcggcccgac gactcctacg gcgcactggt gcggtcgatc
      961 acgggccagc agctgtcggt caaggccgcg cgcaccatct acctgcgcct ggccgacctc
     1021 tacggcgggc gcacccccac cccggccgag ctgatcgcca ccgaccccga cgacctccgc
     1081 gccgtcggcc tcagctaccg gaaggccgcg tacctgcgcg acctggccga gcggatcgag
     1141 agcggcgagc tggccccgga gcgcttcccg gagctggagg accgcgaggt ctcggagatg
     1201 ctgaccgcgg tgaagggcgt cggccagtgg accgccgacg tgttcatgat gttccacctg
     1261 ggccgcgagg acgtactgcc cgtcggggac ctgggcatcc gcaaggcggc ggagatcgag
     1321 tacgggctgc gcaagctgcc ctcgcccgac cggctcaggc gcctggcccg gccctggcgg
     1381 ccgtaccgga cgctggcgtg cgtctacctg tgggagtcgc tggacaacgc accggactag
     1441 ggcgatcctg ggatcgaccc ggaggaggtt gcgatgtacg cacgagtagc gcgttgggag
     1501 ggctccaacg cggaggccct gcggaacatg tccgaaatga tcggctcggc cgagaagccg
     1561 cccgacgggg tgccgtcgaa cggcatcatc gtcctgaacg acctcgagca gggccgctcg
     1621 ctggtgatcg ggctgttcga gaccgaggcg gacctgaaga ccggcgacga ggccctcagg
     1681 gcgatggaca gtccccccga ggccgccggc gacgtgagct cggtcgagtt ctacgaggtc
     1741 gcggtggacc gacgaatgtg acgctctagc gggccggctt gagctcgtgc aggccccacg
     1801 gcgggttggc gttcacgatc cggccgggct tgcaggtggc cacgaacgac gcgaagcgtg
     1861 tccgcggatg gtggccccgg accacgtacc ggccggagcg caccccggtc cagatcacgc
     1921 cgccgtcctt cgacgatcgc cgctgggcgg gatcggggat gacctgctcg ttgaagtaga
     1981 aggggctctt cagcttcggc gtggccgagg ccgtggcgcc cgcgacaccg tgggcgccgt
     2041 agcggacgaa gccgttgaag ctcaggttgc gcaccttgcg cgtgctgaac gtggagacga
     2101 tggcgcacgc acggatgttg cccttcgcgt cgagcggcac cttcagcagc gtggccagcg
     2161 cgttgtacac ggcgtcggtg ggcacctgga agttcacccg ggtgaggtcc ttgcccgcgg
     2221 tggtgaaggt ctgcaggtag atgcggtggt agcccggagc gtcgacgtag ggcgtgaccc
     2281 gcgcgcggtc gggcacggcc agccggtagg cgccgttctt gcgcacggtg gcgcggagcc
     2341 tggggcgctc ggccacgcgg acggtcgcgc cggccaggcg cgtggcgggc ttgttgaact
     2401 cgtacgcggt gccgctcagg tccacggtgg cgccggccgc ggtggcaggc gcggcggcgg
     2461 ccgccagcgt gagcagggcg gcgctagaca gccaggcgga actcgggcgc atattcctcc
     2521 agggggttgg atccgtcgtc gggcatcgtg cgtgagccgt cggccaggcc gaacagcaac
     2581 tcgaagagct cgtcggtgcg cagcgcgggg cgcacctccc agcggacggt gccgtcctgc
     2641 tcggggtggc cgccgaaggt gtggggcgtg cccgccggga tcgtgatccg gtcgccctcg
     2701 accagcatct gctgctcgcc gtcgaccgcc gtcaacaccg cgcccgacag cacctcgaag
     2761 cgctcctcct gggaggggtg caggtgcgcg ggcgcctgcg cgccgccggc cttgtaggag
     2821 gactccatga ccagcaggtc gtcctcgatc gagacgaacg tgatcgtctg acccgtgtgc
     2881 gggttcagga gttcgtcgcc gggcttggcc actgccgctc ggctaggagc cgcagctggg
     2941 gtcttcgtcc gcgatgcgga acgaggagcc gcagccgcag gaggcgacga cgttggggtt
     3001 cttgacctcg aacccggcgc ccatgaggct ctcggtgtag tcgacctgcg agccgtcgac
     3061 gtagcgcatg ctggcgggat ccacgatcag gcggatgccc tcgtggtcga agacctggtc
     3121 ctcttccttc ttctcgtcga gggccagcgc gtactggaag ccggaacagc cgccgccgcg
     3181 cacaccgatg cgcaggccga cttcgccctc ggtctcctgc tgggccagga agccacggac
     3241 cttgtccgct gcgatctcgg tcatcgtcac catgctttcc aaaagatagc aatgggacgg
     3301 gctctggcaa ataacgccat ccgaccccgc tatcttcgtg gtcgtgccga ccgctgagca
     3361 cctcaaggag cgcctcgtgg aagcgttccc ggacgccgac gtcgacgtga gcggcgacga
     3421 cggacagcac ttccgtgccg agatcgtctc ggcccgattc gaggggctca cccgcatcca
     3481 gcagcacaag ctggtccagg aagtgttcgg cggcgagctc ggtggcgcga tccacgccct
     3541 ctcgatcaag acttcgactc cggaaggatg accatggaca acgctgagat ccgcgacttc
     3601 atcgacagct ccatcaagga gaacgaggtg atgctcttca tgaagggcac gcccgaggcc
     3661 ccggcctgcg gcttctccat gcgcacctcc ggcgccctga acgcgctggg cgtccgttac
     3721 gccgccctgg acatcctccc cgacccgcgc atccgcgagg agctctcggg cctctccggc
     3781 gggccgacga tccctcagct cttcgtggac ggcgagctcg tgggcggctc cgacatcgtc
     3841 ctggagatgt tcgagtcggg cgagctggcc gaggtgctcg gtgtggagca gcccg
//
LOCUS       DASUPW010000178         5272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_42018, whole genome shotgun sequence.
ACCESSION   DASUPW010000178 DASUPW010000000
VERSION     DASUPW010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5272
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42018"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VFY44_04095"
     CDS             <1..879
                     /locus_tag="VFY44_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909649.1"
                     /translation="LPLRTVTATAEAVVGLGRLGDPNGPLARLEQLAMLTADDQPLGR
                     ALATGGPLDRLLAEGGVADRLLAEDGPLDRLLAEDGALDRLLAEGGALDRLTAEGGTL
                     DRVLEEQGVLERLFAEDGLIEQMLVVDGTIDRLVQPDGTLNQLLEPGGSIEQLLAEDG
                     PVAHLGDLSETLSRLADLDVTLQRLGALEETLRALGALGDSLNRLATVAAALETLAES
                     TQGLPQLPGRVQAVETQVSSIGDQLGRLEPSLDSLSASVTGLTEMAGRIPGGRKARKA
                     AEKAAREQTEQTEDQK"
     gene            complement(881..1492)
                     /locus_tag="VFY44_04100"
     CDS             complement(881..1492)
                     /locus_tag="VFY44_04100"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX5909650.1"
                     /translation="MPALGDFDAILSDLDGVLVDSDAAIARGYDEWADGHGLDRELVR
                     SLYPGTPARQVVEQAAPWLDPAAEAEAIDRIHVAHGKEVRALPGAAALLDDPPGPLAV
                     VTSCSRILAPARFEAAGLRAPAVVVTADDIEVGKPDPAAYLEGAQRLGIAPATCLVIE
                     DAPAGVRAGKAAGMTVLALTTTHAAGELGEADHLAADLTAREF"
     gene            complement(1503..2132)
                     /locus_tag="VFY44_04105"
     CDS             complement(1503..2132)
                     /locus_tag="VFY44_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5909651.1"
                     /translation="MELAGLIDRALVPPDQADAGDPTRERTLDAAVAEAAAVGLERMT
                     VEDVVRRTGLGRMTVYRRFPRRDDLVEALLLRECRRFLAAVSAALDGDGDPEQRVAAA
                     FTAAMAFVRTHPLTRRAADSEPGAVLDAVAAGDGRILAMGRGFIAAQITAGHPDADPR
                     RVAQVADVVARLFVTYVGIPPEDPDPADEAALADFARRTLVPLLAQAVD"
     gene            complement(2135..3052)
                     /locus_tag="VFY44_04110"
     CDS             complement(2135..3052)
                     /locus_tag="VFY44_04110"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX5909652.1"
                     /translation="MSQLYHPRQWVTKPVTEIMGRHDERELYAQPPGDPGLCGPGSVS
                     WQIHSDLSAIAVAGTAAITMELLHPSVMAGVSDLSTYRTDPLRRARNTAGYVIATTFA
                     GTAAAEDLIARVRRVHGRIAGVRPDGVAYRASDPVLLGWVHTSIPWMVMRAYERYTRP
                     LLPAERDRYLAEQAVIGRLGGAGEIPETVAELDEYVETMRPELAVTEQTREFFDFLVD
                     SDDPLSMPGPLRRPGNAFQLHASLSLLPAWARALTGYDHPAAIRRRVYDPHLRRTART
                     LRWAFGTPPYAGMAAERAAAARAPAAVAA"
     gene            3091..4719
                     /locus_tag="VFY44_04115"
     CDS             3091..4719
                     /locus_tag="VFY44_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX5909653.1"
                     /translation="MSIASEAAGRAMGLPRPLSRDVVVQRDLEVPMPDGALLLADRYA
                     PKPDVPSPTVLVRSPYGRRGPVGLLFGRLLAERGLQAVVQSVRGTFGSGGDFDPFDER
                     DDGLATLEWVGRQPWHEGGIGMTGPSYLGLTQWAVARGAGDALAAMAPSVTASNFHGQ
                     AMGSGAISLETGLSWVTMMAVQERRGAPLHMLRRLRQLPGVIDELPVGGLDALAAGAE
                     VPFYADWLAHTTADDPYWTARDHSADVADVTAPVQLVGGWSDIFLPWMLEDFVALQDA
                     GRSPQLMVGPWTHTAPELARVSAREGIAWLRAHLLGDRRMLDPAPVRVWVGGAREWRS
                     MDTWPPPSQPRALHARAGGRLTEDEPEADEAPDRYRYDPAHPTPSVGGPTLLARDPVT
                     DNTGLEARDDVLVFTTDPLEHDLEVIGPVSAVVHVRADAEHFDVFARVCDVLPSGTSL
                     NVCDVLARVEPGRFDPDADGVHEVLLDLWPTARRFKAGHRIRLQVSSGAHPRYARNLG
                     TGEELATAQRMRAVDVTVLHDAAHPTAVTLPVTA"
     gene            4756..4971
                     /locus_tag="VFY44_04120"
     CDS             4756..4971
                     /locus_tag="VFY44_04120"
                     /inference="COORDINATES: protein motif:HMM:NF022608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2945 domain-containing protein"
                     /protein_id="HEX5909654.1"
                     /translation="MRKYKKGSNVRWSWGNGTGYGQVTDSFTRRVQRTIDGSKIVKNG
                     TDRCPAYLIEQDDGSRVLKLHSEIERS"
     gene            4990..>5272
                     /locus_tag="VFY44_04125"
     CDS             4990..>5272
                     /locus_tag="VFY44_04125"
                     /inference="COORDINATES: protein motif:HMM:NF017809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HEX5909655.1"
                     /translation="MRHLVDFFARRAVPGVEEADGGTYRRSLALAHGDGVLEVRSDGS
                     TGVTLDDERDRPDAEQRLRDLLDLDTDTAPIAGALGDDPLIGGLVRAQPD"
BASE COUNT          660 a         2002 c         1981 g          629 t
ORIGIN      
        1 ctgcccctcc gcacggtcac ggcgaccgcg gaggcggtgg tcgggctggg gcgcctcggc
       61 gatcccaacg ggccgctggc ccggctcgag cagctggcca tgctcaccgc cgacgaccag
      121 ccgctgggcc gcgcccttgc caccggcggc ccgctggacc ggctgctggc cgagggcggg
      181 gtggccgacc ggctgctggc cgaggacgga ccgctggacc ggttgctggc cgaggacggg
      241 gcgctggacc ggctgctggc cgagggcggc gcgctggacc gcctgaccgc cgagggcggc
      301 acgctggacc gcgtgctcga ggagcagggc gtgctggagc gcctgttcgc cgaggacggc
      361 ctgatcgagc agatgctggt cgtggacggc accatcgacc gcctggtcca gcccgacggc
      421 accctgaacc agctgctcga gcccggcggg agcatcgagc agctgctggc cgaggacggg
      481 ccggtggccc acctgggcga cctgtccgag acgctctcgc gcctggccga cctggacgtg
      541 acgctccagc gcctcggcgc gctggaggag acgctgcgcg cgctcggcgc cctgggcgac
      601 tcgctgaacc gcctggccac ggtggccgcc gcgctggaga ccctggccga gtccacccag
      661 gggctgccgc agctgccggg acgggtccag gcggtcgaga cgcaggtctc ctcgatcggc
      721 gatcagctcg gccggctcga gccgtcgctc gactcgctca gcgccagcgt gacggggctg
      781 accgagatgg ccggccggat cccgggcggg cgcaaggcgc gcaaggccgc cgagaaggcg
      841 gcgcgcgagc agaccgagca gaccgaggac cagaagtagc tcagaactcg cgggcggtca
      901 ggtcggcggc caggtggtcg gcctcgccga gctcgcccgc cgcgtgtgtg gtggtcagcg
      961 ccagcacggt catgcccgcc gccttgcccg cgcgcacgcc cgcgggcgcg tcctcgatca
     1021 ccaggcaggt cgcgggcgcg atccccagcc gctgtgcgcc ctccaggtag gccgcgggat
     1081 cgggcttgcc gacctcgatg tcgtcggcgg tcaccaccac ggccggcgcg cgcaggccgg
     1141 cggcctcgaa ccgcgcgggg gccaggatgc ggctgcacga ggtcactacg gccagcggcc
     1201 cgggcgggtc gtccagcagc gccgcggcgc cgggcagcgc ccgtacctcc ttgccgtggg
     1261 ccacgtggat gcggtcgatc gcctcggctt cggccgccgg gtccagccac ggcgccgcct
     1321 gctccacgac ctggcgggcc ggggtgcccg ggtacagcga ccgcaccagc tcgcggtcca
     1381 ggccgtggcc gtccgcccac tcgtcgtagc cgcgcgcgat ggcggcgtcc gagtccacca
     1441 ggacgccgtc caggtcgctc aggatcgcgt cgaagtcgcc cagcgccggc acccggccga
     1501 ggctagtcca cggcctgcgc cagcagcggg accagcgtcc ggcgcgcgaa gtccgcgagg
     1561 gcggcctcgt cggccgggtc ggggtcctcc ggcgggatgc ccacgtaggt cacgaacagc
     1621 cgcgccacca cgtcggccac ctgggcgacc cggcgcgggt ccgcgtccgg gtggccggcc
     1681 gtgatctgcg cggcgatgaa gccgcgcccc atggccagga tgcgcccgtc gcccgcggcc
     1741 accgcgtcca gcacggcccc gggctcggag tcggccgccc ggcgggtcag ggggtgggtg
     1801 cgcacgaacg ccatcgcggc cgtgaacgcc gcggccaccc gctgctcggg atcgccgtcg
     1861 ccgtccaggg cggcggacac cgcggccagg aagcggcggc actcgcgcag caacagcgcc
     1921 tcgaccaggt cgtcccggcg cgggaaccgg cggtacacgg tcatccgccc cagcccggtg
     1981 cggcgcacca cgtcttcgac ggtcatccgc tccaggccca cggccgcggc ctcggccacg
     2041 gccgcgtcca gggtccgctc gcgggtggga tcgccggcgt ccgcctggtc gggcggcacc
     2101 agggcgcgat cgatcagccc cgccagctcc acggctaggc ggccaccgcc gccggcgccc
     2161 gcgccgcggc cgctcgctcg gcggccatcc ccgcgtaggg aggcgtgccg aacgcccagc
     2221 gcagggtccg cgcggtccgg cgcaggtgcg ggtcgtagac ccgccgccgg atcgccgccg
     2281 ggtggtcgta cccggtcagc gcccgcgccc aggccgggag caggctcagg gacgcgtgca
     2341 gctggaacgc gttgccgggg cggcgcagcg ggccgggcat gctcagcggg tcgtcggagt
     2401 cgaccaggaa gtcgaagaac tcgcgcgtct gctcggtgac cgccagctcg gggcgcatcg
     2461 tctccacgta ctcgtccagc tcggcgaccg tctccgggat ctcgccggcg ccgcccaggc
     2521 ggccgatcac cgcctgctcg gccaggtagc ggtcgcgctc ggcgggcagg agcgggcggg
     2581 tgtagcgctc gtaggcgcgc atgaccatcc acgggatcga ggtgtgcacc cagcccagca
     2641 gcacggggtc gctcgcgcgg tacgccaccc cgtcgggccg cacgcccgcg atccgcccgt
     2701 gcacgcgccg gacgcgcgcg atcaggtcct cggccgcggc cgtgccggcg aaggtcgtgg
     2761 cgatcacgta gcccgccgtg ttgcgcgcgc gccgcagcgg gtcggtgcgg taggtggaca
     2821 ggtcgctcac gcccgccatg accgaggggt gcaggagctc catggtgatg gccgcggtcc
     2881 cggcgacggc gatcgcggac aggtccgagt ggatctgcca cgacacgctg cccggtccgc
     2941 acagccccgg gtcgccgggc ggctgcgcgt acagctcacg ctcgtcgtgg cgccccatga
     3001 tctcggtcac gggcttcgtc acccactgcc gggggtggta gagctgagac atgatcagta
     3061 ccagtcatac cacatgccac actcaccggc atgagcatcg ccagcgaagc cgcgggccgg
     3121 gcgatgggcc tgccccgccc gctcagccgc gacgtggtcg tgcagcgcga cctcgaggtg
     3181 cccatgcccg acggcgcgct gctgctggcc gaccgctacg cgcccaagcc cgacgtgccc
     3241 tcgccgacgg tgctggtgcg ctcgccctac gggaggcgcg gcccggtggg gctgctgttc
     3301 gggcggttgc tggccgagcg cgggctccag gccgtggtgc agagcgttcg cggcacgttc
     3361 ggctcgggcg gtgacttcga cccgttcgac gagcgcgacg acggcctggc cacgctggag
     3421 tgggtcggcc gccagccctg gcacgagggc ggcatcggca tgaccgggcc cagctacctg
     3481 ggcctgaccc agtgggcggt ggcccgcggc gccggcgatg cgctggccgc gatggccccc
     3541 tcggtcacgg cctccaactt ccacggccag gccatgggca gcggagcgat ctcgctggag
     3601 acgggcctgt cctgggtcac gatgatggcg gtgcaggagc gccgcggcgc gccgctgcac
     3661 atgctgcgca ggctgcgcca gctccccggt gtcatcgacg agctgcccgt gggcggcctg
     3721 gacgcgctgg ccgccggcgc cgaggtcccc ttctacgccg actggctggc gcacaccacc
     3781 gccgacgacc cctactggac cgcgcgcgac cactccgccg acgtggccga cgtgaccgcg
     3841 ccggtccagc tcgtgggcgg ctggtcggac atcttcctgc cctggatgct cgaggacttc
     3901 gtcgcgctcc aggacgcggg ccggtcgccc cagctgatgg tcggcccgtg gacgcacacc
     3961 gcgcccgagc tggcccgcgt cagcgcccgc gagggcatcg cgtggctgcg cgcccacctg
     4021 ctgggcgacc ggcggatgct cgacccggcg ccggtgcgcg tgtgggtcgg cggcgcgcgc
     4081 gagtggcgga gcatggacac ctggccgccg ccgtcccagc cgcgcgccct tcacgcgcgc
     4141 gcgggcgggc ggctgaccga ggacgagccg gaggcggacg aggccccgga ccgctaccgc
     4201 tacgacccgg cgcacccgac cccctcggtc ggcggcccga cgctgctggc ccgcgacccc
     4261 gtgaccgaca acaccgggct cgaggcgcgc gacgacgtcc tggtgttcac caccgacccg
     4321 ctggagcacg acctcgaggt catcggcccg gtctcggcgg tggtccatgt gcgcgccgac
     4381 gccgagcact tcgacgtgtt cgcgcgcgtg tgcgacgtgc tgccgtccgg gacctcgctg
     4441 aacgtgtgcg acgtgctggc ccgcgtcgag cccggccgct tcgaccccga cgccgacggc
     4501 gttcacgagg tcctgctcga cctgtggccc acggcgcgcc gcttcaaggc cggtcaccgc
     4561 atccggctgc aggtctccag cggagcccac ccgcggtacg cgcgcaacct cggaaccggc
     4621 gaggagctgg ccacggccca gcggatgcgg gccgtggacg tcaccgtgct gcacgacgcc
     4681 gcgcatccca ccgcagtcac gctgccggtg acggcctgag taatcccggc accgccccgc
     4741 atccgtatcg cgggcatgcg caagtacaag aagggctcga acgtgcgctg gtcctggggg
     4801 aacggaaccg gctacggcca ggtcaccgac agcttcaccc ggcgggtcca gcgcaccatc
     4861 gacgggtcga agatcgtcaa gaacggcacc gaccggtgtc cggcgtacct gatcgagcag
     4921 gacgacggca gccgcgtcct caagctgcac tcggagatcg agcgcagctg atctgcgacc
     4981 gtccgggtgg tgcgccacct ggtcgacttc ttcgcgcgca gggcggtgcc cggggtcgag
     5041 gaggccgacg gcggcacgta ccgccgcagc ctggcgctcg cacacggcga cggcgtgctg
     5101 gaggtccgct ccgacggctc caccggcgtc acgctcgacg acgagcgtga ccgccccgac
     5161 gccgagcagc gcctccgcga cctgctcgac ctggacaccg acacggcgcc gatcgccggg
     5221 gcgctgggcg acgacccgct gatcggcggg ctggtccgcg cgcagccgga cc
//
LOCUS       DASUPW010000179         3021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_459375, whole genome shotgun sequence.
ACCESSION   DASUPW010000179 DASUPW010000000
VERSION     DASUPW010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459375"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFY44_04130"
     CDS             complement(<1..462)
                     /locus_tag="VFY44_04130"
                     /inference="COORDINATES: protein motif:HMM:NF024688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEX5909656.1"
                     /translation="MAELLDTIDGPEDLKGLDDAQLAQVAQEVRELIIDTIGEIGGHF
                     GANLGTCEIAVALHSLLESPRDKILWDVGHQAYPHKVLTGRRDQLPTIRKYEGLAPFC
                     SIGESEHDIMGAGHASTSVSYAVGLKEAMRQGRGEDGKVVAVIGDGALTGGV"
     gene            complement(472..1359)
                     /locus_tag="VFY44_04135"
     CDS             complement(472..1359)
                     /locus_tag="VFY44_04135"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX5909657.1"
                     /translation="MSTAAALYPTELQERVDAYLAELPLGGSAQGVETLVEAMRYSLL
                     AGGKRVRPVLALATAEALGHDPESVLPFAGAIEMIHTYSLIHDDLPAMDDDELRRGKP
                     TCHVAYGEDVAILAGDGLFAEALRLAAAEQVADPAALLSAVAELTAAVGVDGMVGGQF
                     LDVTAPGDLGPADLRRVHELKTGRLIAASVNCVLHLCQASPSARLTYGRFAAELGVLF
                     QIVDDILDVTGDDTALGKPSGSDERHGKRTYVTVFGLDRARELARESHSEARAALAES
                     AGDTETLERVADHILTRQN"
     gene            complement(1356..1550)
                     /locus_tag="VFY44_04140"
     CDS             complement(1356..1550)
                     /locus_tag="VFY44_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909658.1"
                     /translation="MAGELDDLVRELEDAATRLRAGELEPKEAAELVDHCARLAVSIG
                     GELEALGRRAENDPDQEQLL"
     gene            complement(1550..1930)
                     /gene="nusB"
                     /locus_tag="VFY44_04145"
     CDS             complement(1550..1930)
                     /gene="nusB"
                     /locus_tag="VFY44_04145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX5909659.1"
                     /translation="MRRSDQRRAAVVALYQADLTNRSAPDLLDARAPEFARELVEGVE
                     RDRAQIDGFISRHSEGWTLDRIAPLERNILRVSLHELIHREDVPAEVSIDEAVEAAKE
                     LCGADAPGYVNGVLGAVHRELVNA"
     gene            complement(1938..2498)
                     /gene="efp"
                     /locus_tag="VFY44_04150"
     CDS             complement(1938..2498)
                     /gene="efp"
                     /locus_tag="VFY44_04150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX5909660.1"
                     /translation="MISTNQLKNGTHIEVDGQVFKVVEFQHVKPGKGPAFVRTKLKRQ
                     SDGNVLDKTFRAGEKFRPIRTETRRMQYLYADGTDAHFMDVETYEQLTIAQEQVADQL
                     RWVRESEEVEVLYVDDKPADVQVPAAVEMAVAETEPGLRGDTASGGGDKPATLESGVV
                     VRVPLFVDVGDKVRVDTRSGEYVSRA"
     gene            2543..>3021
                     /locus_tag="VFY44_04155"
     CDS             2543..>3021
                     /locus_tag="VFY44_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX5909661.1"
                     /translation="MPDLHVLALFAAAAIPVALFPGPAVAFIVTTSLRHGSGFGVRAT
                     AGVESGYVIHVLAAVCGLSALLAASTVAFSAVKLAGAAYLAWLAVGAWRSARRAGDEP
                     VVEEAAAPPAGSAFRQGFAVGALNPKTAVFFLAFLPQFADPSRGPVATQVLVLGLAF"
BASE COUNT          358 a         1125 c         1055 g          483 t
ORIGIN      
        1 cacgccgccg gtcagcgcgc cgtcgccgat cacggcgacg accttgccgt cctcgcccct
       61 gccctggcgc attgcctcct tgaggccgac ggcgtaggag accgacgtgg acgcgtggcc
      121 cgcgcccatg atgtcgtgct cggactcgcc gatcgagcag aacggggcca ggccctcgta
      181 cttgcggatc gtgggcagct ggtcgcggcg ccccgtcagc accttgtgcg ggtaggcctg
      241 gtggccgacg tcccagagga tcttgtcccg cgggctctcc agcagcgagt gcagcgccac
      301 cgcgatctcg cacgtgccca ggttcgcgcc gaagtggccg ccgatctcgc cgatggtgtc
      361 gatgatcagc tcgcgcacct cttgcgccac ctgcgccagc tgcgcgtcgt cgagcccctt
      421 gaggtcctcg gggccgtcga tggtgtccag gagttcagcc atgtcgtggg gtcaattctg
      481 ccgggtaagg atgtggtcgg ctacccgctc cagcgtctcc gtgtcccccg ccgactcggc
      541 aagcgcggcg cgggcctcgg agtgcgactc gcgcgccagc tcgcgggccc gatcgaggcc
      601 gaacacggtc acgtaggtgc gcttgccgtg ccgctcgtcg gatccgctcg gcttgcccag
      661 ggcggtgtcg tctcccgtca cgtcgaggat gtcgtcgacg atctggaaca ggacgcccag
      721 ctctgcggcg aaccggccgt aggtgagtct ggcagacggc gatgcctggc acaggtgcag
      781 cacgcagttc accgaggcgg cgatcagccg gccggtcttc agctcgtgca cgcggcgcag
      841 gtcggccggg cccaggtcac cgggcgcggt cacgtccagg aactgcccgc ccaccatgcc
      901 gtcgaccccg accgcggcgg tcagctcggc caccgcggag agcagcgcag ccgggtcggc
      961 cacctgctcg gcggcggcca ggcgcagcgc ctcggcgaac agcccgtcgc ccgccaggat
     1021 ggccacgtcc tcgccgtagg ccacgtggca ggtgggcttg ccgcggcgca gctcgtcgtc
     1081 gtccatggcc ggcaggtcgt cgtggatcag cgagtaggtg tggatcatct cgatggcgcc
     1141 cgcgaacggg agcaccgact cggggtcgtg gcccagcgcc tcggcggtgg ccagcgccag
     1201 aaccggccgc acgcgcttgc cgccggccag cagcgagtac cgcatggcct cgacgagcgt
     1261 ttccacgccc tgcgcggagc ctcccagcgg caactcggcc aggtacgcgt ccacccgttc
     1321 ctgcagctcg gtcgggtaga gcgcggcggc cgtgctcaca gcagctgttc ctggtccggg
     1381 tcgttctcgg cccggcgccc cagcgcctcc agctcgcccc cgatgctcac ggccaggcgg
     1441 gcgcagtggt ccaccagctc ggccgcctcc ttgggctcca gctcacccgc gcgcagcctg
     1501 gtggccgcgt cctccagctc gcgcaccaga tcgtccagct cgccggccat caggcgttca
     1561 ccaactcccg gtgcacggct ccgagcaccc cgttcacgta cccgggcgcg tcggccccgc
     1621 acagctcctt cgcggcctcc acggcctcgt cgatggacac ctcggcgggg acgtcctcgc
     1681 ggtggatgag ctcgtggagc gacacgcgca ggatgttgcg ttccagcggc gcgatccgat
     1741 caagcgtcca cccctcggag tggcgggaga tgaagccgtc gatctgggca cggtcgcgct
     1801 cgacgccctc gacgagctcg cgggcgaact cgggcgcgcg cgcgtcgagc aggtcgggtg
     1861 cgctgcgatt ggtgaggtcg gcctggtaca gggccaccac cgcggcgcgt ctctggtcgc
     1921 tgcgccgcac ccgtctccta ggcccgcgag acgtattcgc ccgagcgggt gtccacccgg
     1981 accttgtcgc ccacgtccac gaacagcggc acgcgcacca cgacgccgga ctccagcgtg
     2041 gccggcttgt cgccgccgcc cgacgcggtg tcgccgcgca ggcccggctc ggtctcggcc
     2101 acggccatct cgaccgcggc gggcacctgc acgtcggcgg gcttgtcgtc cacgtacagg
     2161 acctcgacct cctcgctctc gcggacccag cgcagctggt cggccacctg ctcctgcgcg
     2221 atggtcagct gctcgtaggt ctcgacgtcc atgaagtggg cgtcggtgcc gtcggcgtac
     2281 aggtactgca tccgccgggt ctcggtccgg atggggcgga acttctcgcc cgcgcggaag
     2341 gtcttgtcca gcacgttgcc gtcgctctgg cgcttgagct tggtgcgcac gaacgcgggg
     2401 cccttgccgg gcttcacgtg ctggaactcg accaccttga agacctggcc gtccacctcg
     2461 atgtgggtgc cgttcttgag ctggttcgtc gaaatcacgt ccggcgaagg ctaccgcccg
     2521 ctcgtgggag gatggggtcc cgatgcccga tctgcacgta ctcgccttgt tcgccgccgc
     2581 ggcgatcccc gtggccctct tccccggccc ggccgtggcc ttcatcgtga ccaccagcct
     2641 gcgccacggc agcggcttcg gcgtgcgcgc cacggcgggt gtggagagcg gctacgtgat
     2701 ccacgtgctg gccgccgtgt gcggcctgtc ggcgctgctg gcggcctcca ccgtggcctt
     2761 ctcggcggtc aagctggcgg gcgcggccta cctggcctgg ctggcggtgg gcgcctggcg
     2821 ctcggcgcgc cgcgccggtg acgagcccgt ggtcgaggag gccgccgctc cgccggcggg
     2881 ctcggccttc cgccagggct tcgccgtggg cgcgctcaac ccgaagacgg cggtgttctt
     2941 cctggccttc ctgccccagt tcgccgaccc ctcgcgcggg ccggtggcca cccaggtgct
     3001 ggtgctgggc ctggccttca t
//
LOCUS       DASUPW010000180         8494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_181398, whole genome shotgun sequence.
ACCESSION   DASUPW010000180 DASUPW010000000
VERSION     DASUPW010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181398"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VFY44_04160"
     CDS             <1..520
                     /locus_tag="VFY44_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX5909662.1"
                     /translation="TAEAVTRLCELGLRPVLLTGDNRATAEAVAAQVGIDDVVAEVLP
                     GDKARVVREHQDAGRVVAMVGDGVNDAPALAQADLGLAIGTGTDVAIEASDLTLVSGD
                     LRAAADAIRLSRATLRTIKQNLGWAFGYNVAAIPLAAVGLLNPVLAGAAMAFSSLSVV
                     ANALRLRAFKAG"
     gene            539..709
                     /locus_tag="VFY44_04165"
     CDS             539..709
                     /locus_tag="VFY44_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909663.1"
                     /translation="MAGYDDDDDWVGEPPEGRYTRDRADPGFWKGQKPVPYLAAAVGL
                     VVLVLLIVLLAG"
     gene            776..1048
                     /locus_tag="VFY44_04170"
     CDS             776..1048
                     /locus_tag="VFY44_04170"
                     /inference="COORDINATES: protein motif:HMM:NF025385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4235 domain-containing protein"
                     /protein_id="HEX5909664.1"
                     /translation="MAKILFTPFSVAFSLIAGLVARKVFDRVWHAIDDEDPPDPGEEL
                     EPLPRILLAAGLQATVFALARALFDRQARKTFRGLTGTWPGPKDKS"
     gene            1171..2520
                     /locus_tag="VFY44_04175"
     CDS             1171..2520
                     /locus_tag="VFY44_04175"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5909665.1"
                     /translation="MSTVGRVAALGAVIAAVVLVALVLFKPGGDQYTLTAQFINAGQL
                     VKGNPVQTGGTPIGSVGDIRITENGQAQVKLNIKEKYAPVPSGTRASIRQFSQSGIAN
                     RYVDLSYPDRKVGTLDDGARLDVDRTSTAVDLDELFNTLDPPTRKALQGFFKGQARQF
                     RGAGTDANEAFRYLNPALSSTSRLFREATRDQPTLERFLVDSSKLVTTLAERRDDLAG
                     LVGNLNDTTRALGDQKVALASSIERLPPFMRRANTTFVNLRAALNDVDPLVDASYRPA
                     RALQPFLTQARAFAADAEPTVRDLRVTVRKPGSANDLIDFVNSIPPLADIAVDPAVRN
                     GKSRRGAFPETVDAFKGGAPQIADARPYTTDFLGWLDDFSTTGGGFDALGAYARGHIS
                     LAENIPAPGSPVKSGQFRRCPGGAEEAAPDGSNVASAEEAQALGCDRFQRATGDVGP"
     gene            2517..4211
                     /locus_tag="VFY44_04180"
     CDS             2517..4211
                     /locus_tag="VFY44_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909666.1"
                     /translation="MRRLIAIVGLLALLGGAAVFAGAKDDEAKAGTEYKIIFDNAFGL
                     TKGGDFRVGGVKAGSTPSFKAVTPKGGPPKAEVTAKITEPGFGDFREDASCNIKPQSL
                     IGEYYVDCQPGSPSKPRLKKGGTVPVSQTSSTIPQDLVNNILRRPYKERLRLVINELG
                     TGLAGRPEDLAAVLRRAHPGLRETSATLRILGNQNRTIENFIRDSDTVVSALEARKQE
                     VTRWIDEAGDTAEITATRRNELASTFNKLPGFLDELDPTMKRLGQLTDEQIPLLADAQ
                     RAAPSLDTFLTRLGPFAQASRPAIRSLGNASEKGTRAFKRGSEEVAELKRLGAQAAPT
                     AKPLAQFLDSLDDRRRAIDNDPRGKVGAPPAGDASNRGQANNGGFTGLESLWTYFFWQ
                     SQSLNAFDNIGHVLRLGITVSDCSPLENRTLQSNPELKAKFDKCNAYLGPNQPGVTTP
                     DFTRTGSAQASNKSKKDGDSGAEPASKVGERRSAGQPDAGPVPGQRDLSKPQIALPPA
                     VQELVDGLTKNTLPEQTKDKLDTEKVDQLLSGEGPKSTTPGAPPSPQQLLDFLLAP"
     gene            4208..5767
                     /locus_tag="VFY44_04185"
     CDS             4208..5767
                     /locus_tag="VFY44_04185"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5909667.1"
                     /translation="MRNRSASATLVASPVLVGAVTLLITIVAVFIAYNANSGLPFVPT
                     YDLKAEVPTGNKLVAGNEVRVGGFRVGVVDEITPKVVRRDGETKTVALLTMKLDKTVE
                     PLAKSTTVKIRPRSALGLKYVQITPGKGKQSFAAGATIPLKNASEGLELEDVYSTLDA
                     KTRRNSQTALDGFGDAFTGRGRALNTSIQALNPFFSFLTPVMSTLNDPETGLADFFPE
                     LGETVGQLAPVAQVQAQVFADMATTFAALSSDPGALQQTIERSPPTIDTAVRSFRVQR
                     PFLADFADLSSRLRPAVNELPRSLPAINRAFKVGTPVLPRTVALNGRLGENFDALDDL
                     FENPNTLLALRDLRTTLTVVRPAMEFVAPYQTVCNYWNYFVYPLGEHQSEVSPLGGTV
                     QQQGARLPNVLQANSLGSIRSSRPVDTPPGVDPVGASVGPEQLQRFYSQPYQPAIDAQ
                     GNADCQVGQNGYIKGPLGPNRYKPGTAPNGDPSGGNAGVSIANFPILSGGTYVSRKLG
                     INNLRDVDKLR"
     gene            5803..7311
                     /locus_tag="VFY44_04190"
     CDS             5803..7311
                     /locus_tag="VFY44_04190"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5909668.1"
                     /translation="MTPFMAGIVGIVVVVFAVFMAFTKANPFSNPYELKGAFETANNI
                     KPNSPVRIAGVEVGKVKKVEAVEEGGKGEGAAIVTMEMKKSALPIHEDATMKIRPRVF
                     LEGNFFVDVKPGSPSSPKLDSGKTIPTTQTAAPVQFGDVLTALQSDTRSDLQVFLKEY
                     SKGLEGPGAEGFNEALRTGPAAFRSTAIASDALLGLEPTKDLQRLFRGQARTFAALNE
                     DENALKGLVTNFNTVAGAFASQDAALERSVPALDRTLRVGSPALASLNSALPTLRVFS
                     REALPGVRSSGPTLEASLPFIRQARLLVGPKELRGTARQLRLQIPNFVTLSKRSQPLL
                     RQARLLSSCTNNVLVPFAETPVPNPDEPRDTGQKPLRQANRGFVGLAGESRVNDGNLS
                     YFRTSAVAPPLNVRPAAPSDGGNQPPPRRPDVPCETQEKPNLNAPGGSILQFPTSSAG
                     ATARAVQARRQIDGPKLQKAGALWRKYNKQTVAPQKKKFDKAVDKLAEESKP"
     gene            7308..>8494
                     /locus_tag="VFY44_04195"
     CDS             7308..>8494
                     /locus_tag="VFY44_04195"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5909669.1"
                     /translation="MKKALKDHLGDFVAILALVILAVGIGGYILSNQRLRFPLVEEKP
                     FVVKVELDDAQAVQPGQGQTVRSAGVEIGEIGKVQLKDGKALVELQLEPEYKSYVKRD
                     ATALLRTKTGLKDMFIEVDPGTGKTLKENETIASASTAPDIDPDEILAALDTDTRSYL
                     QLLVSGAGKGLKGRGSDLQQTYARLGPTNRDLARVNSAVARRKSNLKNLINKYGLLTT
                     ELATKDRQIVRLVRSSNAVFESLATQNNQIASAVNKLPGTLDTTSDTLEKVNTYGQRL
                     TPALQSLRAPIRKLDPANKAVLPLVREATPQIRDQIRPFTRVATPYIRDLGTGAKGLS
                     AGAPDLTVSFDKLNRLFNIGAFNPNGAEGLTGNPVVDRNRQEGYLYWLAWTAQNTNSL
                     FNTA"
BASE COUNT         1411 a         3173 c         2834 g         1076 t
ORIGIN      
        1 cacggccgag gcggtgacgc ggctgtgcga gctgggtctg cggcccgtgc tgctgaccgg
       61 tgacaaccgc gccacggccg aggcggtggc ggcccaggtg ggcatcgacg acgtcgtggc
      121 cgaggtgctg ccgggcgaca aggcgcgggt ggtgcgcgag caccaggacg ccggccgcgt
      181 ggtggccatg gtcggggacg gggtcaacga cgcacccgcg ctggcccagg ccgacctggg
      241 cctggccatc ggcacgggca ccgacgtggc catcgaggcc tccgacctga cgctggtctc
      301 gggcgacctg cgtgccgccg cagacgcgat ccgcctgagc cgcgccacgc tgcggaccat
      361 caagcagaac ctgggctggg ccttcggcta caacgtcgcg gccatcccgc tggcggcggt
      421 cggcctgctg aacccggtgc tggccggagc ggcgatggcg ttctcaagcc tgagcgtggt
      481 ggccaacgcc ctgcgcctgc gcgcgttcaa ggcgggctga gcggggtagt cccctgccat
      541 ggccggctac gacgatgacg acgactgggt gggcgagccg ccggagggcc gttacacgcg
      601 cgaccgcgcc gacccgggct tctggaaggg gcagaagccg gtcccctacc tggccgccgc
      661 ggtcgggctg gtcgtcctcg tactgctgat cgtgctcctc gcgggatgag gcggaaaccg
      721 gccaagggtc gtgggggacc gggggcgtga gcccccggcg atagtctccg ccgccgtggc
      781 gaagatcctt tttaccccgt tcagcgtcgc cttctcgctc atcgcgggcc tcgtggcgcg
      841 caaggtcttc gaccgcgtct ggcacgcgat cgacgacgag gatccgcccg atccgggcga
      901 ggaactcgag ccgttgccgc ggatcctgct ggcggccggg ctgcaggcca cggtgttcgc
      961 gctggcccgc gcgctcttcg accgccaggc gcgaaagacg ttccgcgggc tcaccggcac
     1021 gtggccgggg cccaaggaca agtcttaagc agtctttaac cctttcgccc agccggtgtg
     1081 gtctcacccc gtaaggggta gattccagcg gctgatacga gggcggcgcc tgccgtctca
     1141 gggcggagga acggggaagc gatgggatcg gtgagcacgg tgggaagggt tgccgcactc
     1201 ggtgcggtca tcgctgccgt ggtgctggtc gccctcgtgc tcttcaagcc cggcggcgac
     1261 cagtacacgc tgaccgccca gttcatcaac gccggccagc tggtcaaggg caacccggtg
     1321 cagaccggcg gcacgccgat cggctcggtg ggcgacatcc gcatcaccga gaacggccag
     1381 gcccaggtca agctgaacat caaggagaag tacgcgccgg tgcccagcgg cacccgtgct
     1441 tccatccgcc agttctcgca gtcgggcatc gccaaccgct acgtggacct gtcctacccc
     1501 gaccgcaagg tcgggaccct ggacgacggg gcgcggctgg acgtggaccg gacctcgacc
     1561 gcggtcgacc tggacgagct gttcaacacc ctcgacccgc ccacccgcaa ggcgctccag
     1621 ggcttcttca aggggcaggc gcgccagttc cgcggcgccg gcaccgacgc caatgaggcc
     1681 ttccgctacc tcaacccggc gctctcctcc accagccggc tcttccgcga ggccacccgc
     1741 gaccagccga ccctcgagcg cttcctggtc gacagctcca agctggtgac cacgctggcc
     1801 gagcgccgcg acgacctggc cggcctggtc gggaacctga acgacaccac gcgcgcgctg
     1861 ggcgaccaga aggtggcgct ggccagctcg atcgagcggc tgcccccgtt catgcgccgc
     1921 gccaacacca cgttcgtgaa cctgcgcgcc gcgctgaacg acgtggaccc gctggtggac
     1981 gcctcgtacc gccccgcgcg ggcgctccag ccgttcctga cccaggcccg ggcgttcgcc
     2041 gccgacgccg agcccacggt gcgcgacctg cgcgtgaccg tgcgcaagcc cggctcggcc
     2101 aacgacctga tcgacttcgt gaactcgatc ccgccgctgg ccgacatcgc cgtcgacccg
     2161 gccgtgcgca acggcaagtc ccgccgcggc gccttccccg agaccgtcga cgccttcaag
     2221 ggcggcgcgc cgcagatcgc cgacgcccgg ccctacacca ccgacttcct gggctggctg
     2281 gacgacttct ccaccaccgg cggcgggttc gacgccctgg gcgcctacgc ccgtggccac
     2341 atctcgctgg ccgagaacat ccccgcgccc ggcagcccgg tcaagagcgg tcagttccgc
     2401 cgctgtccgg gcggcgccga ggaggccgca cccgacggct ccaacgtggc cagcgccgaa
     2461 gaggcccagg cgctgggctg tgaccggttc cagcgtgcca ccggggacgt gggcccgtga
     2521 ggcggctgat cgccatcgtc ggcctcctgg ccctgctggg cggcgccgcg gtgttcgccg
     2581 gcgcgaagga cgacgaggcc aaggccggca ccgagtacaa gatcatcttc gacaacgcgt
     2641 tcggcctgac caagggcggc gatttccggg tgggcggcgt caaggccggc tccacgccct
     2701 cgttcaaggc agtcaccccc aagggcggcc cgcccaaggc cgaggtcacg gccaagatca
     2761 ccgagccggg cttcggcgac ttccgcgagg acgccagctg caacatcaag ccccagtcgc
     2821 tgatcggcga gtactacgtg gactgccagc cggggtcgcc gagcaagccg cgcctgaaga
     2881 agggcgggac ggtgccggtc agccagacca gctccaccat cccgcaggac ctggtcaaca
     2941 acatcctgcg ccggccctac aaggagcggc tgcggctggt catcaacgag ctgggcaccg
     3001 ggctcgccgg ccggcccgag gacctggccg ccgtgctgcg gcgagcgcac ccgggactgc
     3061 gtgagaccag cgccacgctg cgcatcctgg gcaaccagaa ccgcacgatc gagaacttca
     3121 tccgcgactc cgacacggtg gtctcggcgc tggaggcgcg caagcaggag gtcacgcgct
     3181 ggatcgacga ggcgggcgac accgcggaga tcaccgccac gcgccgcaac gagctggcct
     3241 cgaccttcaa caagctgccc gggttcctgg acgagctgga cccgacgatg aagcgcctgg
     3301 gccagctcac cgacgagcag atccccctgc tggccgacgc ccagcgggcg gcaccgagcc
     3361 tggacacgtt cctgacccgc ctcgggccgt tcgcgcaggc ctcgcggccg gccatccggt
     3421 cgctgggcaa cgcctccgag aagggcacgc gggcgttcaa gcgcggcagc gaagaggtgg
     3481 ccgagctcaa gcgcctgggc gcgcaggccg cgcccaccgc gaagccgctg gcccagttcc
     3541 tggactcgct ggacgatcgc cgccgcgcca tcgacaacga cccgcgcggc aaggtggggg
     3601 caccgcccgc cggcgacgcg tccaaccgcg gccaggccaa caacggcggc ttcaccggcc
     3661 tggagtcgct gtggacctac ttcttctggc agagccagtc gctgaacgcg ttcgacaaca
     3721 tcggccacgt cctgcggctg ggcatcacgg tcagcgattg cagcccgctg gagaaccgca
     3781 cgctgcagag caaccccgag ctgaaggcca agttcgacaa gtgcaacgcc tacctcgggc
     3841 ccaaccagcc cggcgtgacc accccggact tcacccgcac cggcagcgcg caggccagca
     3901 acaagagcaa gaaggacggc gactccggcg ccgagcccgc ctccaaggtg ggcgagcgcc
     3961 gctccgccgg ccagcccgac gccggccccg tgcccggcca gcgcgacctg tccaagccgc
     4021 agatcgccct gcccccggcg gtccaggagc tggtcgacgg cctgaccaag aacaccctgc
     4081 ccgagcagac caaggacaag ctggacaccg agaaggtgga ccagctgctc agcggtgagg
     4141 gccccaagag caccacgccc ggcgcgcctc cgagcccgca acagctcctc gacttcctcc
     4201 tggcgccatg aggaaccggt ccgccagcgc aaccctcgtg gccagcccgg tgctggtggg
     4261 cgccgtcacg ctgctgatca cgatcgtggc cgtcttcatc gcctacaacg ccaactccgg
     4321 cttgccgttc gtgccgacct acgacctcaa ggccgaggtg cccaccggca acaagctggt
     4381 ggccggcaac gaggtccgcg tcggcggctt ccgcgtcggc gtggtggacg agatcacccc
     4441 gaaggtggtc cgcagggacg gcgagaccaa gaccgtcgcg ctcctgacga tgaagctgga
     4501 caagaccgtc gagccgcttg cgaagagcac cacggtgaag atccggcccc ggtcggcgct
     4561 gggcctgaag tacgtgcaga tcacgcccgg caagggcaag cagtcgttcg ccgccggcgc
     4621 cacgatcccg ctgaagaacg ccagcgaggg cctggagctg gaggacgtgt actccacgct
     4681 ggacgccaag acccggcgca actcgcagac cgcgctcgac gggttcggcg acgccttcac
     4741 cggccgcggc cgcgcgctga acacctcgat ccaggccctg aaccccttct tctcgttcct
     4801 gacgccggtc atgagcacgc tcaacgaccc ggagacgggc ctggccgact tcttccccga
     4861 gctgggcgag acggtgggcc agctggcgcc ggtggcgcag gtccaggccc aggtgttcgc
     4921 cgacatggcc accacgttcg cggcgctcag ctcggacccg ggcgcgttgc agcagaccat
     4981 cgagcgctcg ccgccgacca tcgacaccgc cgtgcgctcg ttccgcgtcc agcgtccctt
     5041 cctggccgac ttcgccgacc tgtccagccg tctgcgcccg gccgtgaacg aactgccgcg
     5101 atcgctgccc gccatcaacc gagcattcaa ggtcgggacg ccggtgctgc cgcgcaccgt
     5161 tgcgctgaac ggccgcctgg gcgagaactt cgacgcgctg gacgacctgt tcgagaaccc
     5221 gaacacgctg ctggcgctgc gcgacctgcg caccacgctg accgtggtcc ggccggcgat
     5281 ggagttcgtg gcgccctacc agacggtctg caactactgg aactacttcg tctacccgct
     5341 gggcgagcac cagtccgagg tcagcccgct gggcggcacg gtccagcagc agggcgcccg
     5401 gctgcccaac gtcctgcagg ccaactcgct gggctcgatc cgcagctcgc ggccggtgga
     5461 cacgccgccc ggcgtcgacc ccgtgggcgc cagcgtcggc cccgagcagc tgcagcgctt
     5521 ctacagccag ccctaccagc cggccatcga cgcccagggc aacgccgact gccaggtggg
     5581 ccagaacggc tacatcaagg gcccgctcgg acccaaccgc tacaagccgg ggacggcccc
     5641 caacggtgat cccagcggcg gcaacgccgg ggtcagcatc gccaacttcc cgatcctgtc
     5701 cggcggcacc tacgtgtcac gcaagctggg catcaacaac ctccgcgacg tggacaagct
     5761 gcgctgatgg cccgccagaa gaaacgccgg gacaagcggg gcatgacgcc tttcatggcg
     5821 ggcatcgtcg gcatcgtcgt cgtggtcttc gccgtgttca tggccttcac gaaggccaac
     5881 ccgttctcga acccctacga gctcaagggc gcgttcgaga ccgccaacaa catcaagccc
     5941 aactcgcccg tgcgtatcgc cggggtcgag gtgggcaagg tcaagaaggt cgaggcggtc
     6001 gaggagggcg ggaagggcga gggcgcggcg atcgtgacga tggagatgaa gaagtcggcg
     6061 ctgcccatcc acgaggacgc gacgatgaag atccgcccgc gcgtgttcct ggagggcaac
     6121 ttcttcgtgg acgtcaagcc cgggtcgccg tcgtcgccca agctggacag cggcaagacg
     6181 atccccacca cgcagaccgc ggcgccggtg cagttcggcg atgtgctgac cgccctccag
     6241 agcgacaccc ggtccgacct ccaggtgttc ctgaaggagt actccaaggg cctcgagggc
     6301 ccgggtgccg agggcttcaa cgaggcgctg cgcaccggcc cggcagcgtt ccgcagcacg
     6361 gccatcgcca gcgacgccct gctgggactc gagcccacca aggacctgca gcgcctgttc
     6421 cgcggccagg cgcgcacctt cgccgcgctg aacgaggacg agaacgcgct caagggcctg
     6481 gtcaccaact tcaacaccgt cgccggcgcc ttcgccagcc aggacgcggc gctggagcgc
     6541 tcggtcccgg ccctggaccg gacgctgcgc gtcggctcgc ccgcgctggc ctcgctcaac
     6601 tcggccctgc ccaccctgcg cgtgttctcc cgcgaggcgc tgcccggcgt gcgctccagc
     6661 ggcccgaccc tggaggcctc gctgcccttc atccgccagg cccggctgct tgtcggtccc
     6721 aaggagctgc gcggcaccgc ccgccagctg cgcctgcaga tcccgaactt cgtcacgctc
     6781 agcaagcgct cccagccgct gctgcgccag gcgcggctgc tgtcctcgtg caccaacaac
     6841 gtgctggtgc ccttcgccga gaccccggtg cccaaccccg acgagcccag ggacacgggc
     6901 cagaagccgc tgcgccaggc caaccgcggc ttcgtcggcc tggccggcga gagccgcgtg
     6961 aacgacggca acctgtccta cttccgcacc agcgcggtcg cgccgcccct gaacgtgcgc
     7021 ccggcggctc cctccgacgg tggcaaccag cccccgccgc ggcggcccga cgtgccctgt
     7081 gagacccagg agaagcccaa cctgaacgcg cccggcggca gcatcctgca gttccccacc
     7141 agcagcgccg gcgccaccgc ccgggcggtc caggcgcgca ggcagatcga cgggcccaag
     7201 ctccagaagg ccggcgccct gtggcgcaag tacaacaagc agacggtcgc gccgcagaag
     7261 aagaagttcg acaaggccgt cgacaagctg gccgaggaga gcaagccgtg aagaaggccc
     7321 tgaaagacca cctcggggac ttcgtcgcca tcctggcgct ggtcatcctg gccgtcggga
     7381 tcggcggcta catcctgtcc aaccagcggc tgcgcttccc cctcgtggag gagaagccgt
     7441 tcgtggtcaa ggtcgagctc gacgacgccc aggcggtgca gccgggccag ggccagaccg
     7501 tgcgcagcgc cggcgtcgag atcggcgaga tcggcaaggt ccagctcaag gacggcaagg
     7561 cgctggtgga gctccagctc gagcccgagt acaagagcta cgtcaagcgc gacgccaccg
     7621 cgctgctgcg caccaagacc gggctcaagg acatgttcat cgaggtcgac ccgggcaccg
     7681 gcaagacgct gaaggagaac gagacgatcg cctccgccag cacggcgccc gacatcgacc
     7741 cggacgagat cctggccgcg ctggacaccg acacccgctc ctacctccag ctgctggtgt
     7801 cgggcgccgg caagggcctg aagggccgcg gcagcgacct ccagcagacc tacgcccgcc
     7861 tcgggcccac caaccgcgac ctggcccgcg tgaactcggc cgtggcccgg cgcaagagca
     7921 acctcaagaa cctgatcaac aagtacgggc tgctgaccac cgagctggcc accaaggacc
     7981 gccagatcgt gcgcctggtg cgctcctcga acgcggtgtt cgagtcgctg gccacgcaga
     8041 acaaccagat cgccagcgcg gtgaacaagc tgccgggcac gctggacacc accagcgaca
     8101 cgcttgagaa ggtcaacacc tacggccagc ggctgacccc ggcgctgcag tcgctgcgcg
     8161 cgcccatccg caagctcgac cccgcgaaca aggcggtgct gccgctggtg cgcgaggcca
     8221 cgccccagat ccgcgaccag atccggccgt tcacgcgcgt cgccacgccc tacatccgcg
     8281 acctcggcac gggcgccaag ggcctcagcg ccggcgcccc cgacctgacg gtgtcgttcg
     8341 acaagctcaa ccggctgttc aacatcggcg ccttcaaccc caacggcgcc gagggcctga
     8401 ccggcaaccc cgtggtcgac cgcaaccgcc aggagggcta cctgtactgg ctggcctgga
     8461 ccgcgcagaa caccaactcg ctgttcaaca cggc
//
LOCUS       DASUPW010000181         3809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1289680, whole genome shotgun sequence.
ACCESSION   DASUPW010000181 DASUPW010000000
VERSION     DASUPW010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289680"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VFY44_04200"
     CDS             complement(<1..520)
                     /locus_tag="VFY44_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909670.1"
                     /translation="MRRRDLIVRAAGVVLTGELVVHQLRYLIVPASQDAHGYLPLLGV
                     ASVVALAAGAGHLAATLERARRTGRDERVALGFLRAWGMVVAGVLALFWAQETFEALL
                     LGAALAAPLAPLTGGGWVALPLALALGAVLALALTGARAAVRAAAQRARAGVERVRPR
                     RRRLPEAALLVSF"
     gene            complement(554..1357)
                     /locus_tag="VFY44_04205"
     CDS             complement(554..1357)
                     /locus_tag="VFY44_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX5909671.1"
                     /translation="MNLSDQGPIRLAVLDSDTGFVRVLENRLEGLDWPHLHLEAAPRV
                     EEMAAMRVDALVLDLALLGRDAWDYVEQLAAAAPTLGIVVCTGRSTVAQRVRGLRAGA
                     DDWVSKPCHPEEVIARVEAVVRRRTHASGFGDDAPVTAGDLEIRADQFQAFSGGRSAD
                     LTRREFEVLTLLAHAEGKVLQREEIYQRVWGYTMAHGDRSVDVFIRKVRQKLEAVSPP
                     WRYIHTHFGVGYRFDPELRDAGAPEPQAPAMGDAALDEAFEQITAGLPD"
     gene            1472..1822
                     /locus_tag="VFY44_04210"
     CDS             1472..1822
                     /locus_tag="VFY44_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5909672.1"
                     /translation="MEAATNGNLQAGEVVVRPSEYMAAADGRPLNLTSRELELLTALV
                     ERQGRIVSREELHRSVWGEPYRKSDRSVDVYIAKLRQKLDDAAPDRRFIHTHFGFGYR
                     FQPEETRSHLSRLS"
     gene            1946..2905
                     /locus_tag="VFY44_04215"
     CDS             1946..2905
                     /locus_tag="VFY44_04215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5909673.1"
                     /translation="MNKWLALAATGVLALGVAACGDDSGSGGGGGGGKDLSGSIKIDG
                     SSTVGPFAQAAAEGFQGENPNVKVSVGTSGTGGGFEKFCAGETDISDASRPIKDDEEV
                     PLCKKGGVKYKEIQVANDGIAVVSNKDLKIDCMTTDELKKLWNQGSKVTAYNQVNPKF
                     PSTKASLFGPGTDSGTFDFFTDEINGEEGVSRKEYQPSEDDNVLVQGVEGDKGGLGYF
                     GFSYYEQNQDKLNLIGIDGGDGCVKPSSKSIQDNSYKPLSRPLFMYPSEKSLARPEVK
                     AFMDYLVENQDEIAKTSQIVPLTEAQKTKGANELKAAEAAAGK"
     gene            2909..>3809
                     /gene="pstC"
                     /locus_tag="VFY44_04220"
     CDS             2909..>3809
                     /gene="pstC"
                     /locus_tag="VFY44_04220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX5909674.1"
                     /translation="MEAATQTTRATPKLGVARRRIGEDVIKGVLLLAALLSVLTTTGI
                     VLSLVRETIVFFEDVPIADYLFGTDWSPVIKPQSFGVLPLVWGTLFVTAIALVVAIPL
                     GLGSAIYLSEYATPRVRRIFKPILEMLAGVPTIVFGYFALTFFTPNLLNDLAKLDVSV
                     FNVLSASIVMGFMIVPTIASVSEDAMSAVPQSLREGSFGLGASRLQTSLRVVFPAALS
                     GIVASVVLGASRAVGETMIVLVAAGQVAQINADPREPEFTMASYIASIAKGDLATGSV
                     QYKTVFAVGMLLFLITLVLNAFSI"
BASE COUNT          626 a         1341 c         1308 g          534 t
ORIGIN      
        1 agaaggacac gagcagcgcc gcctcgggga gccgccgccg gcgcggacgc acgcgctcca
       61 cgcccgcgcg ggcgcgctgc gccgcggcgc gaaccgcggc ccgggcgccg gtcagggcca
      121 gcgccagcac cgcgccgagg gcgagcgcca gcggcagcgc cacccagccg cccccggtca
      181 ggggcgccag cggcgccgcc agggcggcgc ccagcagcag cgcctcgaag gtctcctggg
      241 cccagaacag cgccagcacg ccggccacga ccatgcccca ggcgcgcagg aagcccagcg
      301 ccacgcgctc gtcgcggccg gtgcggcgcg cccgttccag ggtggcggcc aggtggcccg
      361 cgcccgcggc cagggccacc accgaggcca cgccgagcag cggcaggtag ccgtgcgcgt
      421 cctgggacgc cggcacgatc aggtagcgca gctggtgtac gacgagctcg ccggtgagca
      481 cgaccccggc ggcgcggacg atcaggtcac ggcggcgcat cccgccgcag tatggcgaac
      541 gagggaggaa gccctagtcg ggcaggccgg cggtgatctg ctcgaacgcc tcgtccaggg
      601 cggcatcgcc catcgcgggc gcctgcggct ccggggcgcc ggcgtcgcgg agctcggggt
      661 cgaaccggta gccgacgccg aagtgggtgt ggatgtagcg ccaggggggc gagaccgcct
      721 ccagcttctg gcgcaccttg cggatgaaga cgtcgaccga ccggtcgccg tgggccatcg
      781 tgtagcccca gacgcgctgg tagatctcct cgcgctggag caccttgccc tcggcgtggg
      841 ccagcagcgt caggacctcg aactcgcgcc gggtcaggtc ggcgctgcgg ccgcccgaga
      901 aggcctggaa ctggtcggcg cggatctcca ggtcgccggc ggtcacgggc gcgtcgtcgc
      961 cgaagcccga cgcgtgtgtg cggcggcgca ccacggcctc gacccgggcg atgacctcct
     1021 ccgggtgaca gggcttggag acccagtcgt cggcgcccgc gcgcagcccg cgcacgcgct
     1081 gggcgacggt cgagcgaccg gtgcagacca cgatgcccag ggtgggcgcg gcggcggcca
     1141 gctgctcgac gtagtcccag gcgtcacggc ccagcagggc caggtccagg accagcgcgt
     1201 cgacgcgcat ggcggccatc tcctccacgc ggggagcggc ttccaggtgc aggtggggcc
     1261 agtcgaggcc ttcgagccgg ttctccagca cgcgcacgaa tccggtgtcg gaatcgagca
     1321 cggccaggcg gatcggtcct tgatcgctca ggttcacgtg tgcccagcat acgccacagc
     1381 cccgtgccgt tacgcgcgtt tacaggtttt tcacaggccg gtcacgcgcg cataacggcg
     1441 gttccgcaca tagctggcag cttgcactgc catggaagcg gcgaccaacg ggaacctgca
     1501 ggcgggcgag gtcgtggtgc ggccctccga gtacatggcc gcggccgacg gccggccgct
     1561 gaacctgacg tcgcgtgagc tcgagctcct gacggcgctg gtcgagcgcc agggacgcat
     1621 cgtcagccgc gaggaactgc accgcagcgt gtggggcgag ccctaccgca agtccgaccg
     1681 ctcggtggac gtctacatcg ccaagctgcg ccagaagctg gacgacgcgg cgcccgaccg
     1741 gcgcttcatc cacacccact tcgggttcgg ctaccgcttc cagcccgagg aaacgcggag
     1801 ccacctttcg cgcctctcct agggcgaaaa gccctatttg cgggctttca cattcctttc
     1861 acatggtgtg cacaacccgg caacagactg ccggatacgc catgcgacct tcgccccaca
     1921 catgaataac tcaaggagaa atcgcatgaa caagtggctc gcgctggccg cgacgggcgt
     1981 gctggcactg ggagtcgccg cctgtggcga cgactccgga agtggcggcg gaggcggagg
     2041 cggcaaggac ctctccggct cgatcaagat cgacggctcc agcaccgtgg gccccttcgc
     2101 ccaggctgcg gccgagggct tccagggtga gaaccccaac gtgaaggtgt ccgtgggcac
     2161 ctcgggcacc ggcggcggct tcgagaagtt ctgcgccggc gagaccgaca tctccgacgc
     2221 ctcgcgtccg atcaaggacg acgaggaggt cccgctctgc aagaagggcg gcgtgaagta
     2281 caaggagatc caggtggcca acgacggcat cgccgtggtc agcaacaagg acctgaagat
     2341 cgactgcatg accaccgacg agctgaagaa gctctggaac cagggctcca aggtcacggc
     2401 ctacaaccag gtcaacccga agttcccgag caccaaggcc agcctgttcg gcccgggcac
     2461 ggactccggc acgttcgact tcttcaccga cgagatcaac ggtgaagagg gcgtctcgcg
     2521 caaggagtac cagccgtccg aggacgacaa cgtgctggtc cagggcgtcg agggcgacaa
     2581 gggcggcctc ggctacttcg gcttctccta ctacgagcag aaccaggaca agctgaacct
     2641 gatcggcatc gacggcggcg acgggtgcgt caagccctcc tccaagtcga tccaggacaa
     2701 cagctacaag ccgctgtccc ggccgctgtt catgtacccc agcgagaagt cgctggcccg
     2761 gcccgaggtg aaggccttca tggactacct ggtcgagaac caggacgaga tcgcgaagac
     2821 ctcgcagatc gtccccttga ccgaggccca gaagaccaag ggcgccaacg agctcaaggc
     2881 ggcagaggcc gcagccggca agtaaccgat ggaagcggcg acccagacca cgcgtgccac
     2941 ccccaagctg ggggtggcac gccgccgcat cggcgaggac gtgatcaagg gggtcctcct
     3001 cctcgcggcc ctcctgtcgg tgctgaccac caccggcatc gtgctctccc tggtgcggga
     3061 gacgatcgtc ttcttcgagg acgtcccgat cgccgactac ctcttcggca ccgactggtc
     3121 gccggtgatc aagccccaga gcttcggcgt cctgccgctg gtgtggggca cgctgttcgt
     3181 gaccgccatc gccctggtgg tggccatacc gctgggcctg ggctcggcga tctacctgag
     3241 cgagtacgcc accccgcggg tgcgccggat cttcaagccc atcctggaga tgctggccgg
     3301 cgtgccgacg atcgtgttcg gctacttcgc gctgaccttc ttcacgccga acctgctgaa
     3361 cgacctggcc aagctggacg tgagcgtgtt caacgtcctg tcggcctcga tcgtcatggg
     3421 gttcatgatc gtcccgacga tcgcgtcggt ctccgaggac gccatgtccg cggtgccgca
     3481 gtcgctgcgc gagggctcgt tcggcctcgg cgccagccgc ctccagacct ccctgcgcgt
     3541 ggtctttccc gccgccctgt cgggcatcgt cgcctccgtc gtgctgggcg cctcgcgcgc
     3601 cgtcggcgag acgatgatcg tcctggtggc cgcggggcag gtcgcccaga tcaacgccga
     3661 cccccgggag cccgagttca ccatggcttc gtacatcgcc tcgatcgcca agggcgatct
     3721 ggccaccggg agcgtgcagt acaagaccgt gttcgcggtc gggatgctgc tgttcctgat
     3781 cacgctggtc ctgaacgcct tctccatcc
//
LOCUS       DASUPW010000182         7582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1289912, whole genome shotgun sequence.
ACCESSION   DASUPW010000182 DASUPW010000000
VERSION     DASUPW010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7582
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289912"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..1471)
                     /locus_tag="VFY44_04225"
     CDS             complement(155..1471)
                     /locus_tag="VFY44_04225"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5909675.1"
                     /translation="METRYTRSGDVHIAYQAMGHGPALLYVPTWVSQMEQLGSEPRMA
                     TFIERLCSFARLITFDRRGSGLSDPVEGAPTLEEQIDDILAVMEAVGTERTALVASLE
                     GGPLAITFAASHPDKVSSLILYATFARTRWAEDYDFPPRDHERGTRLDRVIAQWGQGA
                     IPRALAPSRAGDPAFADWAGRMERYSASPGTIRKMFDTIAATDVRHVLPTLSVPTLVM
                     HRRDDAFLMVEHSRYLAEHIPGARYVELEGTDSLFSEGDSEAILGEIEEELTGSRHAP
                     EPDRVLATVMFTDIVDSTRHAAGMGDARWRELLARQERLTRAEVERHRGRTIKSTGDG
                     VLATFDGPARAIRAAQAVRDGSSGLGLEVRAGLHTGECELVGDDVAGLAVHIGARVMG
                     LAGAGEVLTSSTVKDLVAGSGIDFGERGEHELRDVPGRWRLFSVGR"
     gene            1548..1727
                     /locus_tag="VFY44_04230"
     CDS             1548..1727
                     /locus_tag="VFY44_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909676.1"
                     /translation="MAVPALVAGVALALAGVLVWALLRREAAHRAVIAEMAAREERFR
                     TLATQAPGGLYEADL"
     gene            1734..4472
                     /locus_tag="VFY44_04235"
     CDS             1734..4472
                     /locus_tag="VFY44_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5909677.1"
                     /translation="MTFASERFCQMAGRRPDQITGTSWLDLVHPDDVELVRENWSRSV
                     QEGTTFELEYRLRHPDGQVRWATGRNTELYDEQGALRGFAGSSLDVTARHAAEEDRRL
                     RAEITAHMSEGVVLVRHSDNQIVFANRRLEEMFGYAEGQMLGMSASRLNADGRGDVAS
                     TVATILAAVERDGAWRGEIYNVRRNGTRFWTWANVSAFEHRDHGMVSLSIQSDVTERR
                     RVDEELRNERRLLAEAQAIGHVGSWEIKLSDATATWSEEMVRLHGMPAGSRPPRRYLD
                     LVHPDDRADVRARAVAMVQQPEEFEIEYRIVGMDGRERMIQVHGAPVADGTPRLAGTA
                     RDVTTARESERALRVAEERFHSAFADAPIGMALTTVDGTFHEVNQALADLTGYDADEL
                     VGSHFSTITHPDDVAADAERIQAVIDGRAETFQNEKRYVHASGELVWVSVHGVAIRDA
                     DGRPVHLLKQVVDVSERRSYEAKLQHMADHDPLTGLLNRRSFERELEKHMTRVGRYGA
                     EGAAIVLDVDHFKYVNDTHGHNVGDELIVRVAQMLRERLRDTDVLARLGGDEFAVLLP
                     REKRESATAVAEDLLEQVRTVTSPGGSGKRPRRLTASVGVAAFTGEDGLTGEDVLVNA
                     DLAMYDAKEAGRDQVSVSRGDGLGEARMKGRVTWAERIRRALEEDRFVLLAQPIVDLA
                     SGKASQYELLLRMRDDTGDLIPPGAFLAIAERLDLIQDIDRWVTTRAIELLGEYHDAG
                     MKVTFEVNLSGRSIGDPALLELIEQRLAQTGVSPSWLIFEITETSAVANIPRAREFGE
                     RLSELGCRFALDDFGAGFGSFYYLKHLPFDYLKIDGEFVRNCVTNATDRLIIESVVGI
                     ARGLHKLTIAEFVGDEETVRMLDDLGVDFAQGYHTGKPAPLEELIGAKGAVARSPV"
     gene            4537..6180
                     /locus_tag="VFY44_04240"
     CDS             4537..6180
                     /locus_tag="VFY44_04240"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEX5909678.1"
                     /translation="MPKRKASDVFVECLEAEGVKHVFGIPGEETLDLNESLSRSSIDF
                     VPVRHEQGGGYMADTYGRLTGNAGVCLGTLGPGALNLTTPVADAYLDRAPLVALTGQG
                     DLERMHKESHQYIDLVRVMAPITKWNARLNDPHIIPEVVRKAFKIAEGEKPGSTHIEL
                     PEDVMEAEIDAGPLPRGRTTRPEPQARELLKAADVVRAATNPVALAGNGVIRANATPA
                     LREFVRATGIPVAETFMGKGLIPVDSPKALGSVGLQSGDYSMAGFDEADVVLAIGYDL
                     VEHSPEHWNGSKDKKIICIDSLPAEVDEYFVPEVELIGDIYHTLSRLAEECRHVPHQG
                     GSPRLREVVHGRFDAAKDDDHFPVQPPRALYEIRKALDREDILVSDVGLHKLWIGRMF
                     PAHEPNTVLIANGLAGMGFALPAAIAAKIVHPDRKVVTVSGDGGFLMNNQELETAVRL
                     RTPIVNVIWENQQFGSIVWKQDKKFGQHFGTDFTNPDFVKLAESFGLPAWRVGSADEF
                     GQRLTHALGLDLPSVIVLPIDYSIDVAISEELGHETTAT"
     gene            6221..>7582
                     /locus_tag="VFY44_04245"
     CDS             6221..>7582
                     /locus_tag="VFY44_04245"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX5909679.1"
                     /translation="MASTEQQVVDAVPKQLFIGGQWRDGTGGGTIDVEDPATGETLTA
                     VADATPEDAEAALAAADSAQAEWAATPPNERSRILQDAFELLNERADDLALLMTLEMG
                     KTLVESKGEITYAAEFFRWNAGEALRIDGQYKVAGNAASRVLTMRQPIGPSLMITPWN
                     FPMAMGTRKIGPAIAAGCTMVVKPAKLTPLSMLALAGILEEVGLPGGVLNVVTAASSG
                     STMGPLIADPRTRKLSFTGSTEVGRTLIEQSAGQVLKVSMELGGNAPFVVFEDADLDA
                     AVDGAMVAKMRNIGEACTSANRFHVAAAVAEEFAQKLAERMGAMKVARGTEDGAEVGP
                     LIDDDQRGKVAELVDDAVDKGAEALTGGKAVDGPGYFYEPTVLSGISDDARLLKEEIF
                     GPVAPIIPFDSEDEAVAAANDTEFGLVAYVYTSDLKRGIRVCESLETGMIGLNQGLGS
                     NAGA"
BASE COUNT         1157 a         2755 c         2680 g          990 t
ORIGIN      
        1 ggcgaacacg gtcaggtcga tgcggcccgc gcggctgatg tcggcgccgg tgaagacctt
       61 ctcgggcggg gcctgggcgt cggcgcccgg cacgaacagg aggccggcga aagccatcac
      121 gagactcacc ctccggcgcg acaccgccag caggctagcg cccgacgctg aacagccgcc
      181 agcgccccgg cacgtcgcgc agctcgtgct cgccgcgctc gccgaagtcg atgcccgagc
      241 ccgcgaccag gtccttgacc gtgctggagg tcagcacctc gccggcgccg gccaggccca
      301 tcacgcgcgc cccgatgtgc acggccagcc cggccacgtc gtcgcccacc agctcgcact
      361 cgccggtgtg cagccccgcc cgcacctcca gccccaggcc ggacgagccg tcgcggaccg
      421 cctgcgccgc gcggatggcg cgcgccggcc cgtcgaaggt ggccagcacg ccgtcaccgg
      481 tggacttgat ggtgcgccca cggtggcgct cgacctcggc ccgggtcagg cgctcctggc
      541 gggccagcag ctcgcgccag cgcgcgtcgc ccatcccggc cgcgtggcgg gtggagtcca
      601 cgatgtcggt gaacatcacg gtggccagga cccggtcggg ctcgggcgcg tgacgcgatc
      661 cggtcagctc ctcctcgatc tcgccgagga tggcctcgct gtcgccctcc gagaacagcg
      721 agtcggtgcc ctccagctcc acgtaccggg cccccgggat gtgctcggcc aggtagcgcg
      781 agtgctcgac catcaggaac gcgtcgtcgc gccggtgcat caccagggtg ggcaccgaca
      841 gcgtgggcag cacgtgccgc acgtcggtcg ccgcgatggt gtcgaacatc ttgcggatcg
      901 tccccgggct ggcggagtag cgctccatcc ggcccgccca gtcggcgaag gccgggtcgc
      961 cggcgcggct gggcgccagc gcgcgcggga tggcgccctg gccccactgg gcgatcacgc
     1021 ggtccaggcg cgtgccgcgc tcgtggtcgc gcggcgggaa gtcgtagtcc tcggcccagc
     1081 gcgtgcgcgc gaaggtggcg tacaggatca gcgagctgac cttgtccggg tggctggcgg
     1141 cgaaggtgat ggccagcggg ccgccctcca gcgaggccac cagcgccgtc cgctcggtgc
     1201 cgaccgcctc catcacggcc aggatgtcgt cgatctgctc ctccagcgtt ggcgccccct
     1261 ccaccgggtc ggacaagccg gacccgcgcc ggtcgaaggt gatcaaccgc gcgaacgagc
     1321 acaggcgctc gatgaacgtg gccatcctcg gctcggagcc cagctgctcc atctgcgaca
     1381 cccaggtggg cacgtagagc agcgccggcc cgtggcccat ggcctggtag gcgatgtgca
     1441 cgtcgccgct gcgcgtgtac cgggtctcca cggcagcagg ctaccgccgc gccggtgtgc
     1501 gaaaacccct tcctaaaggg gacacctggg gtgccgatag accggacatg gccgttcccg
     1561 cccttgtggc cggtgtcgcg ctggcgctgg ccggcgtgct ggtctgggcg ctgctgcgcc
     1621 gggaggccgc ccaccgggcc gtgatcgcgg agatggcggc cagggaggag cggttccgga
     1681 ccctggccac gcaggcgccc ggcgggctct acgaggccga cctctagggc cgcgtgacct
     1741 tcgccagcga gcggttctgc cagatggcgg gccgcaggcc cgaccagatc acggggacat
     1801 cctggctcga cctggtgcac ccggacgacg tcgagctggt ccgcgagaac tggagccgct
     1861 cggtccagga gggcaccacg ttcgagctgg agtaccggct ccggcacccg gacggacagg
     1921 tgcgctgggc caccggccgg aacaccgagc tctacgacga gcagggcgcg ctccgcggct
     1981 tcgccggcag cagcctggac gtgacggcgc gccacgccgc ggaggaggac cgccgcctgc
     2041 gcgcggagat caccgcccac atgtccgagg gcgtcgtgct ggttcgccac tccgacaacc
     2101 agatcgtgtt cgccaaccgg cgcctcgagg agatgttcgg gtacgccgag ggccagatgc
     2161 tgggcatgag cgcgtcgcgg ctgaacgcgg acgggcgcgg cgacgtcgcc tccaccgtgg
     2221 cgaccatcct ggccgcggtc gagcgcgacg gggcgtggcg cggcgagatc tacaacgtgc
     2281 gccgcaacgg cacgcgcttc tggacctggg cgaacgtctc ggcgttcgag caccgggacc
     2341 acggcatggt gtcgctctcc atccagagcg acgtgaccga gcgccggcgg gtcgacgagg
     2401 agctccgcaa cgagcgccgg ctgctggccg aggcgcaggc catcggccac gtgggcagct
     2461 gggagatcaa gctctccgat gccacggcca cgtggtcgga ggagatggtg cgactgcacg
     2521 gcatgccggc ggggtcgcgg cccccgcgcc gctacctcga cctggtgcac ccggacgacc
     2581 gcgcggacgt gcgggcccgg gcggtggcca tggtccagca gccggaggag ttcgagatcg
     2641 agtaccggat cgtgggcatg gacggccgcg agcgcatgat ccaggtgcac ggcgcgccgg
     2701 tcgccgacgg cacgccacgg ctcgccggga cggcgcgcga cgtgaccacc gcccgcgagt
     2761 ccgagcgcgc cctgcgcgtg gccgaggagc gcttccacag cgccttcgcc gacgccccga
     2821 tcggcatggc tctgaccacc gtcgacggca ccttccacga ggtcaaccag gcgctggccg
     2881 acctcaccgg ctacgacgcg gacgagctgg tgggctcgca cttcagcacc atcacccatc
     2941 ccgacgacgt ggccgccgac gccgagcgga tccaggccgt catcgacggc cgggccgaga
     3001 cgttccagaa cgagaagcgc tacgtgcacg cgtcgggcga gctggtgtgg gtgtccgtcc
     3061 acggggtcgc catccgcgac gccgacggcc ggcccgtgca cctgctgaag caggtcgtgg
     3121 acgtctccga gcggcgcagc tacgaggcca agctccagca catggccgac cacgaccctc
     3181 tgaccgggct gctcaaccgg cgcagcttcg agcgcgagct cgagaagcac atgacgcgcg
     3241 tgggccgcta cggcgccgag ggcgccgcca tcgtcctgga cgtcgaccac ttcaagtacg
     3301 tgaacgacac ccacggccac aacgtgggcg acgagctcat cgtccgcgtg gcccagatgc
     3361 tgcgcgagcg cctgcgcgac accgacgtgc tggcccgcct gggcggcgac gagttcgccg
     3421 tgctgctgcc gcgcgagaag cgcgagagcg ccaccgccgt ggccgaggac ctgctcgaac
     3481 aggtgcgcac ggtgacctcg ccgggcgggt caggcaagcg cccgcggcgg ctaaccgcca
     3541 gcgtgggcgt ggccgccttc accggcgagg acgggctgac cggggaggac gtgctggtca
     3601 acgccgacct ggccatgtac gacgccaagg aggccgggcg cgaccaggtc tcggtgtcac
     3661 gcggcgacgg cctcggcgag gcgcgcatga agggacgggt cacgtgggcc gagcgcatcc
     3721 gccgcgccct ggaggaggac cgcttcgtgc tgctggccca gccgatcgtg gacctggcca
     3781 gcggcaaggc cagccagtac gagctgctgc tgcgcatgcg cgacgacacc ggcgacctga
     3841 tcccgccggg cgcgttcctg gccatcgccg agcgcctgga cctgatccag gacatcgacc
     3901 gctgggtcac cacccgcgcg atcgagctgc tgggcgagta ccacgacgcg ggcatgaagg
     3961 tcaccttcga ggtcaacctc tccgggcgct cgatcggcga cccggcgctg ctggagctca
     4021 tcgagcagcg gctggcccag accggcgtgt cgcccagctg gctgatcttc gagatcaccg
     4081 agacgtcggc cgtggccaac atcccgcgtg cccgcgagtt cggcgagcgc ctgtccgagc
     4141 tgggctgccg cttcgcgctg gacgacttcg gcgccggttt cggctcgttc tactacctca
     4201 agcacctgcc gttcgactac ctcaagatcg acggcgagtt cgtccgcaac tgtgtcacca
     4261 acgccaccga ccggctgatc atcgagagcg tggtcggcat cgcccgcggg ctgcacaagc
     4321 tgaccatcgc cgagttcgtg ggcgacgagg agacggtcag gatgctcgac gacctgggcg
     4381 tggacttcgc ccagggctac cacaccggca agcccgcccc gctcgaggag ctgatcggcg
     4441 caaagggcgc cgtcgccaga tcccctgtgt aggtcctcgg gcgtgctcag ccgatcggcg
     4501 atcggcttgc gcgcggcccg ctgtagtgtc ccggcggtgc caaagcgcaa ggcgtcggac
     4561 gtgttcgtgg agtgcctgga ggccgagggg gtcaagcacg tcttcggcat ccccggtgag
     4621 gagaccctgg acctgaacga gtcgctctcg cgctcgtcga tcgacttcgt gcccgttcgc
     4681 cacgagcagg gcgggggcta catggccgac acctacggcc gcctgaccgg caacgccggc
     4741 gtgtgcctgg gcacgctcgg cccgggcgcg ctgaacctga ccacgcccgt ggccgacgcc
     4801 tacctggacc gtgcgccgct ggtggcgctg accggtcagg gcgacctgga gcgcatgcac
     4861 aaggagtcgc accagtacat cgacctggtg cgcgtgatgg cgcccatcac caagtggaac
     4921 gcgcgcctga acgaccccca catcatcccc gaggtcgtgc gcaaggcgtt caagatcgcc
     4981 gagggcgaga agcccggctc cacccacatc gagctgcccg aggacgtcat ggaggccgag
     5041 atcgacgccg ggccgctccc gcgcgggcgc acgacccgcc ccgagcccca ggcgcgcgag
     5101 ctgctcaagg ccgccgacgt ggtccgcgcc gccaccaacc cggtcgcgct ggccggcaac
     5161 ggcgtgatcc gcgccaacgc cacgcccgcg ctgcgcgagt tcgtgcgcgc caccggcatc
     5221 ccggtggccg agaccttcat gggcaagggc ctgatccccg tcgactcgcc caaggcgctc
     5281 ggctcggtcg gcctccagtc gggcgactac tccatggccg gcttcgacga ggccgacgtg
     5341 gtgctggcca tcggctacga cctggtcgag cactcacccg agcactggaa cggctccaag
     5401 gacaagaaga tcatctgcat cgactcgttg ccggccgagg tcgacgagta cttcgtgccc
     5461 gaggtcgagc tgatcggcga catctaccac acgctctcgc ggctggccga ggagtgccgc
     5521 cacgtgcccc accagggcgg atcgccgcgc ctgcgcgagg tcgtgcacgg ccgcttcgac
     5581 gcggccaagg acgacgacca cttccccgtc cagcccccgc gggcgctgta cgagatccgc
     5641 aaggcgctcg accgcgagga catcctggtc tccgacgtcg gcctgcacaa gctgtggatc
     5701 gggcgcatgt tcccggccca cgagcccaac accgtgctca tcgccaacgg cctggccggc
     5761 atgggcttcg cgctccccgc cgccatcgcg gccaagatcg tccaccccga ccgcaaggtc
     5821 gtgaccgtct ccggcgacgg cggcttcctg atgaacaacc aggagctcga gaccgccgtc
     5881 cgcctgcgca ccccgatcgt gaacgtgatc tgggagaacc agcagttcgg gtcgatcgtc
     5941 tggaagcagg acaagaagtt cggccagcac ttcgggaccg acttcaccaa cccggacttc
     6001 gtcaagctgg ccgagtcgtt cgggctgccc gcctggcgcg tcggctcggc cgacgagttc
     6061 ggccagcgcc tgacccacgc gctgggcctg gacctgccct cggtgatcgt gctgccgatc
     6121 gactactcga tcgacgtggc catctccgag gagctgggcc acgagaccac cgcgacctag
     6181 ccgctgatcg aacccgccgg ccccgggtag cgttgggcgc atggcttcca ccgaacagca
     6241 ggtcgtcgac gccgtcccca agcagctctt catcggaggc cagtggcgtg acggcaccgg
     6301 cggcggcacc atcgacgtcg aggacccggc caccggcgag acgctgaccg cggtcgccga
     6361 cgccacaccc gaggacgccg aggcggcgct ggcggcggcg gactcggccc aggccgagtg
     6421 ggcggcgacg ccccccaacg agcgcagccg catcctccag gacgcgttcg agctcctgaa
     6481 cgagcgcgcc gacgacctgg cgctgctcat gaccctggag atgggcaaga ccctggtcga
     6541 gtccaagggc gagatcacct acgcggccga gttcttccgc tggaacgcgg gcgaggcgct
     6601 gcgcatcgac ggccagtaca aggtcgccgg caacgccgcc agccgcgtgc tgaccatgcg
     6661 ccagccgatc gggcccagct tgatgatcac cccctggaac ttcccgatgg ccatgggcac
     6721 gcgcaagatc gggccggcca tcgccgcggg ctgcacgatg gtggtcaagc ccgccaagct
     6781 gacgccgctg tccatgctcg ccctggccgg gatcctggag gaggtcggcc tgccgggcgg
     6841 cgtcctgaac gtggtcaccg ccgcctccag cggcagcacg atgggcccgc tgatcgccga
     6901 cccgcgcacc cgcaagctgt ccttcaccgg ctccaccgag gtcggccgca cgctcatcga
     6961 gcagtcggcc gggcaggtgc tgaaggtgtc gatggagctc ggcggcaacg cccccttcgt
     7021 ggtgttcgag gacgccgacc tggacgccgc ggtcgacggg gcgatggtcg ccaagatgcg
     7081 caacatcggc gaggcctgca cgtcggccaa ccgcttccac gtggccgccg cggtggccga
     7141 ggagttcgcg cagaagctgg ccgagcgcat gggcgcgatg aaggtggcgc gcggcaccga
     7201 ggacggcgcc gaggtcggcc cgctgatcga cgacgaccag cgcggcaagg tggccgagct
     7261 ggtggacgac gcggtcgaca agggcgccga ggcactgacc ggcggcaagg cggtcgacgg
     7321 tcccggctac ttctacgagc cgaccgtgct cagcggcatc agcgacgacg cgcgcctgct
     7381 caaggaggag atcttcggcc cggtcgcgcc gatcatcccg ttcgactccg aggacgaggc
     7441 cgtcgcggcg gccaacgaca ccgagttcgg cctcgtggcc tacgtgtaca cgtccgacct
     7501 caagcgcggc atccgcgtct gcgagtcgct ggagacgggg atgatcggcc tgaaccaggg
     7561 gctcggatcc aacgcgggcg cc
//
LOCUS       DASUPW010000183         2925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1152260, whole genome shotgun sequence.
ACCESSION   DASUPW010000183 DASUPW010000000
VERSION     DASUPW010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152260"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="VFY44_04250"
     CDS             complement(<1..1131)
                     /locus_tag="VFY44_04250"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5909680.1"
                     /translation="MSAKAFASLAEGESARWEHQISSADVDAFAALSGDDNPLHMDDA
                     FARQQGYRGRVVHGMLLGAYLSRVVGTMLPGPGVVWLSQEVRFVGPAYIGDTVEVAVR
                     ITHKSPALRTLVLESTITNQHGDKLVTGEGKMMMLAQKTTVPWTEMVALVTGGSRGIG
                     ASVARALGDRGATVVVNYHERRDPAEEVVAAIEAAGGRALAVQADIADEQATVALADA
                     ALEAFGRVDAVVNNATPPIERKPLEELSWAEVDRYWRSYVQSAFLLSERLVPGMKERG
                     FGRFVHMLTTATWGTPPRDVAGYVAAKSGLWGLARSMAVELAPHGITVNGVSPSAVIT
                     DQWAEASDNRRRALAMSVPAQRLASPEEVASTVLFLLGEEGSY"
     gene            complement(1128..1367)
                     /locus_tag="VFY44_04255"
     CDS             complement(1128..1367)
                     /locus_tag="VFY44_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX5909681.1"
                     /translation="MRDQVREVVGQVFGLSPADIADDASIETVEGWDSLHHMELMLEL
                     EMRFGVQMTTDVMPTLISIPAIEEYLQEQGAGPRA"
     gene            complement(1360..>2925)
                     /locus_tag="VFY44_04260"
     CDS             complement(1360..>2925)
                     /locus_tag="VFY44_04260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HEX5909682.1"
                     /translation="QALNPSSELYADGPDGVLLIPAAEDVLAELFDDPGSASDQTALV
                     DERLAEIEASVTAILDRLPAATCHVVAYGADRAPVEHVLDPGDPARGQGAVDRWVDGL
                     RGLSRLSPRVVIVDWERHVRRIGSAGLTDPRLWYLGRMRLNPDGLAELAELTARQVAA
                     YRGGAHKVAVVDLDGTLWGGVVGEAGVSGLELGQEGAGLAFRDFQAELARLRATGIVL
                     AVSSKNNRDEAVEAIETHPAMALRLSDFAAERINWADKATSLREMAQELNLGLDSFVF
                     LDDNPVERDWVRQALPEVTVPDLPEDPAHRPAFLRECGLFDRIVLTDADTARAQSYAA
                     QGSRTRLREGSMSFEDFLRSLEQEVTIEAVGDATLERAAQLCQRTNQFNLTTRRHTAA
                     DIERMMASDTHQLYTLAVSDRFGQSGVTGLAILELDGADATIDTLLLSCRVLGRRVED
                     AFLSFLADRARAGGARTLVGMYVPTDRNGQTAGFFPDRGFEDAGEGRFALDLTESAVP
                     MPAEMDVRVGADA"
BASE COUNT          388 a         1117 c         1015 g          405 t
ORIGIN      
        1 gtacgagccc tcctcgccga gcaggaacag cacggtcgag gccacctcct ccgggctggc
       61 cagccgctgg gcgggcacgc tcatggccag ggcgcggcgg cggttgtccg aggcctcggc
      121 ccactggtcg gtgatcacgg ccgacggcga cacgccgttc acggtgatcc cgtgcggcgc
      181 cagctccacg gccatgctcc gggccaggcc ccacaggccg ctcttggccg ccacgtagcc
      241 ggccacgtcg cggggcggcg tcccccacgt ggccgtggtc agcatgtgca cgaagcgccc
      301 gaacccccgc tccttcatgc ccggcaccag ccgctcggac agcaggaacg cgctctgcac
      361 gtaggagcgc cagtaccggt ccacctcggc ccagctgagc tcctcgagcg gcttgcgctc
      421 gatcggcggc gtcgcgttgt tgaccaccgc gtccacgcgc ccgaaggcct ccagcgcggc
      481 gtccgccagc gcgaccgtgg cctgctcgtc cgcgatgtcg gcctggaccg ccagcgcccg
      541 gccgcccgcc gcctcgatgg cggccacgac ctcctcggcc gggtcacggc gctcgtggta
      601 gttgaccacc acggtggcgc cgcggtcgcc cagggcgcgc gccaccgagg cgccgatgcc
      661 ccggctgccg cccgtgacca gggccaccat ctccgtccag ggcactgtgg tcttctgggc
      721 gagcatcatc atcttccctt ccccggtgac gagcttgtcg ccgtgctggt tggtgatcgt
      781 ggactcgagc acgagcgtac gcagcgccgg gctcttgtgg gtgatgcgga cggccacctc
      841 gacggtgtcg ccgatgtacg cgggcccgac gaaccggacc tcctgggaga gccagacgac
      901 gcccggcccc gggagcatgg tcccgaccac ccgcgacagg taggcgccga ggagcatccc
      961 gtgcacgacc cggccgcggt agccctgctg gcgggcgaac gcgtcgtcca tgtgcagcgg
     1021 gttgtcatcg cccgaaagcg ccgcgaaggc gtccacgtcg gcgctggaga tctggtgttc
     1081 ccaccgcgcg ctctctccct ccgccagcga ggcgaacgcc ttcgcgctca tgcccgaggg
     1141 ccggccccct gctcctgcag gtactcctcg atcgcgggga tggagatcag cgtgggcatc
     1201 acgtcggtgg tcatctgcac gccgaagcgc atctccagct ccagcatcag ctccatgtgg
     1261 tgcagcgagt cccagccctc gacggtctcg atgctggcgt cgtcggcgat gtccgccggc
     1321 gacaggccga agacctgccc caccacctcc ctgacctggt cacgcatcgg cgcccaccct
     1381 aacgtccatc tccgcgggca tcgggaccgc ggactcggtc agatcgagcg cgaacctgcc
     1441 ctcgccggcg tcctcgaacc cgcggtcggg gaagaagccg gcggtctggc cgttgcggtc
     1501 ggtggggacg tacatcccga ccagcgtccg cgcgccgccg gcgcgggcgc gatcggccag
     1561 gaacgacagg aaggcgtcct cgacccgccg tcccagcacc cggcagctga gcagcaacgt
     1621 gtcgatggtg gcgtcggctc cgtccagctc gaggatcgcc aggccggtga cgccgctctg
     1681 gccgaaccgg tcgctcacgg cgagcgtgta cagctggtgc gtgtccgagg ccatcatccg
     1741 ctcgatgtcg gccgcggtgt gccggcgcgt ggtcaggttg aactggttgg tgcgctggca
     1801 cagctgcgcc gcccgctcca gcgtggcgtc cccgaccgcc tcgatggtca cctcctgctc
     1861 cagggagcgc aggaagtcct cgaacgacat gctgccctcg cgcaggcgcg tgcggctgcc
     1921 ctgcgccgcg tagctctggg cccgcgccgt gtcggcgtcg gtgagcacga tccggtcgaa
     1981 caggccgcac tcgcgcagga aggcgggccg gtgggccggg tcctccggca gatcgggcac
     2041 cgtgacctcc ggcagcgcct ggcgcaccca gtcgcgctcc acggggttgt cgtccaggaa
     2101 gacgaacgag tccaggccca ggttcagctc ctgcgccatc tcgcgcaggc tcgtggcctt
     2161 gtccgcccag ttgatgcgct cggccgcgaa gtcggacagc cggagcgcca tggcggggtg
     2221 ggtctcgatg gcctccaccg cctcgtcccg gttgttcttg ctggacacgg caagcacgat
     2281 gcccgtggcc cgcaggcgcg ccagctcggc ctggaagtca cggaacgcca ggcccgcacc
     2341 ctcctggccc agctcgagcc cggacacgcc ggcctcgccg accacgccgc cccacagcgt
     2401 gccgtccagg tcgaccaccg cgaccttgtg cgcgccgccg cggtacgccg cgacctggcg
     2461 cgcagtcagc tcggccaact cggccaggcc gtcggggttc agccgcatcc ggccgaggta
     2521 ccagagccgc gggtcggtca ggcccgcgct gccgatgcgg cgcacgtgcc gctcccagtc
     2581 cacgatcacc acccgcggcg agagccgcga gaggccgcgc aggccgtcca cccagcggtc
     2641 gaccgcaccc tggccccggg ccgggtcgcc ggggtccagc acgtgctcca cgggcgcgcg
     2701 gtccgcgccg tacgcgacca cgtggcaggt ggcggcgggc aggcggtcca ggatcgccgt
     2761 caccgaggcc tcgatctcgg ccagccgctc atcgaccagg gcggtctggt cggaggcgct
     2821 tcccgggtcg tcgaacagct cggccaggac gtcctcggcg gcggggatca gcaggacccc
     2881 gtcgggcccg tccgcgtaca gctccgagga agggttcagc gcctg
//
LOCUS       DASUPW010000184         2596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1983432, whole genome shotgun sequence.
ACCESSION   DASUPW010000184 DASUPW010000000
VERSION     DASUPW010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1983432"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VFY44_04265"
     CDS             complement(<1..329)
                     /locus_tag="VFY44_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909683.1"
                     /translation="MEIVLIIVAVVVVIVLIAAIVGGNKRKKVKNQQRRVEARDHREE
                     AEVRDARAQAREAEAEQQAAIAKREQAKAEEQRVHAQRERKAADERHEHADQIDPDVD
                     AKARREER"
     gene            complement(395..1189)
                     /locus_tag="VFY44_04270"
     CDS             complement(395..1189)
                     /locus_tag="VFY44_04270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5909684.1"
                     /translation="MSVLDRPPTGRDAVHHHESDEQLLERYAQGSLEAREELARRHMP
                     MARRLAARHRRSSEATEDLEQVAYLGLLKTIDRYDPGLGSFVGYAVTTIRGELKRHFR
                     DHGWTMHVTRPVQERYLLVSAAADSLGASQGHPPTVKELAAETGLTLDEVIEALDAAH
                     GYSPPSLDAPAGADPDDPSRTLADTVGLVERGYDRVELGEAIGPAFRQLPARQQDMLR
                     MRFIEDLTQSEIAARCGVSQMHVSRLLRRSLNELLDTVGPEGRIPA"
     gene            1476..1856
                     /locus_tag="VFY44_04275"
     CDS             1476..1856
                     /locus_tag="VFY44_04275"
                     /inference="COORDINATES: protein motif:HMM:NF021568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na-translocating system protein MpsC family
                     protein"
                     /protein_id="HEX5909685.1"
                     /translation="MAPSSSAPDESRLQGGELLAAISNQIVRLLRDATGRGPTQCKTF
                     WAGDEMILVQIKGGYSAAEHTLADAGHEDEVHSSRRALQDSVEEQMRSIVETQVGRPV
                     IAFMSNTHLDPDMMIEVFALGPES"
     gene            1901..2155
                     /locus_tag="VFY44_04280"
     CDS             1901..2155
                     /locus_tag="VFY44_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor"
                     /protein_id="HEX5909686.1"
                     /translation="MAENHGPSVKDDEQYEALRREGASKEKAARIANTDRQTAGKRGG
                     QSESYEDWTKDDLESRAAELDIDGRSDMSKDQLIDALRNH"
     gene            complement(2130..>2596)
                     /locus_tag="VFY44_04285"
     CDS             complement(2130..>2596)
                     /locus_tag="VFY44_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909687.1"
                     /translation="CDTGTDFAKGFYRRPFRAGGQVADHGGGRVTELTAKPLLRRFFP
                     ELADSLQPLAGEVAATRQLLASVPFVTGYGVDIALLIDAWRAVGVDRMAQVDLDVRQN
                     RHQPLADLGPMASAVLDAVISRLLAQSGAPGVEERPPMDRRPRPVSDYAARR"
BASE COUNT          366 a          904 c          940 g          386 t
ORIGIN      
        1 cgctcctcgc ggcgggcctt ggcgtctacg tcgggatcga tctggtcggc gtgctcgtgc
       61 cgctcgtcgg cggccttgcg ctcgcgctgg gcgtggacac gctgctcctc ggccttggcc
      121 tgctcgcgct tggcgatcgc ggcctgctgc tcggcctcgg cctcgcgggc ctgagcgcgc
      181 gcatcccgga cctccgcttc ctcgcggtgg tcgcgcgcct cgacgcgacg ctgctggttc
      241 ttgaccttct tgcgcttgtt gcctccgacg atcgcggcga tcaggacgat caccactacg
      301 acggcgacga tgatcaggac gatttccatg gttgctgctc ctctcctcgg gtgggcggcc
      361 tccccgcctc gaacctcgga gatacccggg accgtcaggc cggaatccgg ccctccggcc
      421 cgaccgtgtc cagcagctcg ttcagggacc ggcgcaggag gcgcgagacg tgcatctgcg
      481 agaccccgca gcgggcggcg atctcggact gcgtcaggtc ctcgatgaag cgcatgcgca
      541 gcatgtcctg ctgacgggcg ggcagctgac ggaacgcggg gccgatggcc tcgcccagct
      601 cgacccggtc gtacccgcgc tccaccaggc cgaccgtgtc ggccagggtc cgggaggggt
      661 cgtcggggtc ggcgccggcc ggggcgtcca gggacggcgg cgagtagccg tgcgcggcgt
      721 ccagggcctc gatgacctcg tccagggtca gcccggtctc ggcggccagc tccttgacgg
      781 tcggggggtg gccctgcgag gcgccgagcg agtcggccgc ggcgctgacc agcaggtagc
      841 gctcctgcac gggacgggtc acgtgcatcg tccagccgtg gtcgcggaag tggcgcttga
      901 gctcgccgcg gatggtggtc accgcgtagc ccacgaacga gcccaggccc gggtcgtagc
      961 ggtcgatggt cttcagcagg cccaggtacg cgacctgctc caggtcctcc gtggcctcgc
     1021 tgctgcggcg atggcgcgca gccaggcggc gggccatcgg catgtgccgc cgggccagct
     1081 cctcgcgcgc ttccagcgag ccctgcgcat agcgctcgag cagctgctcg tcgctttcat
     1141 ggtggtggac tgcatcccgc ccggtgggcg gtcgatcgag gactgacatt cggttcctaa
     1201 cgcgtcgaac aagtcgatcg caggatccga ggcgctggcc gagacgacca ggtgcgtccc
     1261 ctacgtactc cgggggccga gcgccgcaaa gcgacctcga ctctgtgtca gagggtatca
     1321 atcgtcacgc cccactccac ccttcgcgtt tgcgggagcg ccgctccggt caggtccccc
     1381 ttgcggcact tgccccgtcc cagttcgcag cctgagggaa cggtgtgctg agggcgcggg
     1441 tcggggtcgc accaggacgg taggatttcc ggtctatggc accgtcctcg agcgcccccg
     1501 acgagtcccg tctgcagggc ggcgagctgc tcgccgcgat ctcgaatcag atcgtgcggt
     1561 tgctccgcga cgccacgggc cgcggcccga cgcagtgcaa gacgttctgg gccggcgacg
     1621 agatgatcct ggtccagatc aagggcgggt actcggcggc cgagcacacg ctggccgacg
     1681 ccggccacga ggacgaggtc cactccagcc ggagggcgct ccaggacagc gtggaggagc
     1741 agatgcgcag catcgtcgag acgcaggtcg gccgcccggt gatcgcgttc atgagcaaca
     1801 cccacctgga ccccgacatg atgatcgagg tcttcgcgct cgggcccgag tcctgaccca
     1861 ccgcgggttt tggccgcgcc gtcacggata gtcgtacggc atggctgaga accatggacc
     1921 aagcgtgaag gacgacgagc agtacgaggc gctgcggcgc gagggcgcca gcaaggagaa
     1981 ggcggcgcgg atcgccaaca cggaccgcca gaccgccggc aagcgcggcg ggcagtccga
     2041 gagctacgag gactggacca aggacgacct ggagtcacgg gcggccgagc tggacatcga
     2101 cggccgctcg gacatgtcca aggaccagct catcgacgcg ctgcgtaatc actgaccggc
     2161 cgggggcgcc tgtccattgg cgggcgttcc tcgacgccgg gcgctccgga ctgcgccagc
     2221 aggcggctga tcaccgcgtc cagtaccgct gaggccatcg gcccgaggtc cgccagcggc
     2281 tggtggcggt tttggcgcac gtccaggtcg acctgggcca tccggtcgac gccgaccgcg
     2341 cgccacgcgt cgatcagcag cgcgatgtcc accccgtagc cggtcacgaa cggcaccgac
     2401 gccagcagct gccgcgtggc ggccacctcg ccggccagcg gttggaggct gtccgccagc
     2461 tcggggaaga accggcgcag caggggcttg gccgtgagct cggtcacccg gccgccgccg
     2521 tggtcggcga cctggccgcc ggcccggaag gggcggcggt agaagccctt ggcgaagtcg
     2581 gtcccggtgt cgcagg
//
LOCUS       DASUPW010000185         3053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_875943, whole genome shotgun sequence.
ACCESSION   DASUPW010000185 DASUPW010000000
VERSION     DASUPW010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875943"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VFY44_04290"
     CDS             complement(<1..287)
                     /locus_tag="VFY44_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5909688.1"
                     /translation="MSRLEGKVAVITGTASGIGAEMARVFRENGATVVGVDLNEGDAG
                     DLNIRADVTDEQQVAGMYGRTKQEFGRVDVIVNNAGINPPEDMSVLDTPVET"
     gene            complement(284..1633)
                     /locus_tag="VFY44_04295"
     CDS             complement(284..1633)
                     /locus_tag="VFY44_04295"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5909689.1"
                     /translation="MISVVEPATAGVMAEVPRAGADDVDAAVERAKAAFPAWRAVEPA
                     ERSAVLRRLADAVEERLEDLAVLEARNAGKPIGDARGEMGMVVETFRYYAGAPERLLG
                     DTIPVAGGIDMTFREPLGVVGLIVPWNFPLVIAAWKLGPALAAGNTVVLKPAELTPLT
                     ALELEKIAATVLPEGVLNVVAGPGSVCGSRLVEHPDVAKVAFTGSTEVGRGIARQAAD
                     TIKRVTLELGGKSANIVFADADLEAAAAAAPMSVFGNAGQDCCARSRILVQRDAMDRF
                     TELLDAAVREVRVGDPLDESTQMGPLISAAHREEVASFVPEGAPVATRGSAPEGDGFW
                     YPPTVLAPVDQHDRAAVEEIFGPVVCVIAFDDEADAVAKANDTIYGLSGSVWTGDGAK
                     ALRVARAVDTGVLSINSNTSVRVSTPFGGFKQSGVGRELGPHALDHYTEVKNVYFAT"
     gene            complement(1630..2352)
                     /locus_tag="VFY44_04300"
     CDS             complement(1630..2352)
                     /locus_tag="VFY44_04300"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX5909690.1"
                     /translation="MTVAVGICAAIEKVRWGAWDTEVHLTPRNYARAVQDADAVALIL
                     PPDDHALEDPARLLDHVDALILAGGADVDPASYGARPHEQTRGSWPERDRFELALTHA
                     ALERDMPVLGICRGMQMLNVACGGTIDQHLPDSLGHDDHRHTPGTFGDHDVRVEPGSL
                     AEQAAGGTSIPVKSHHHQGVAELGEDLVVTGWSEPDQVIEAIELPGRPYALGVLWHPE
                     EDLRANVVKSLVEAAERAKAAA"
     gene            complement(2349..>3053)
                     /locus_tag="VFY44_04305"
     CDS             complement(2349..>3053)
                     /locus_tag="VFY44_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEX5909691.1"
                     /translation="EINFHYGEALRVCDDHAIYKNGAKEIADQEEMAITFMAKFNELE
                     GNSCHIHCSLGSEDGANVFGEDRPTFERFVAGQLAAMRELTMFFAPHINSYKRFADGS
                     FAPTAVAWGEDNRTCAIRAVGHGPSLRFENRLPGADVNPYLALAAMIAAGLKGIDEEM
                     ELEPALEGNAYTAGRERVPSTLRDARELFAGSELAREAFGQEVVDHYLNHADVELKAF
                     DAAVTDWEKVRGFERL"
BASE COUNT          391 a         1131 c         1078 g          453 t
ORIGIN      
        1 gtctcgaccg gggtgtccag cacgctcatg tcctcgggcg ggttgatgcc ggcgttgttg
       61 acgatcacgt ccacgcgccc gaactcctgc ttggtgcgcc cgtacatgcc ggccacctgc
      121 tgctcgtcgg tcacgtcggc gcggatgttc aggtcgccgg cgtcaccctc gttgaggtcc
      181 accccgacca ccgtcgcacc gttctcgcgg aacacgcgcg ccatctcggc gccgatgccg
      241 gacgccgtcc cggtgatcac ggccaccttg ccctcgagcc tgctcatgtc gcgaagtaca
      301 cgttcttgac ctcggtgtag tggtccagcg cgtgcgggcc cagctcgcgg ccgacgccgg
      361 actgcttgaa gccgccgaag ggcgtgctga cccgcaccga ggtgttggag ttgatcgaca
      421 gcaccccggt gtccacggcg cgggcgacgc gcagcgcctt ggccccgtcg cccgtccaca
      481 ccgagcccga gagcccgtag atggtgtcgt tggccttggc caccgcatcc gcctcgtcgt
      541 cgaaggcgat cacgcacacc accgggccga agatctcctc gaccgcggcg cggtcgtgct
      601 ggtcgaccgg cgcgagaacc gtcggcgggt accagaaccc gtcgccctcg ggtgcgctgc
      661 cgcgggtggc caccggcgcg ccctcgggca cgaacgacgc cacctcctcg cggtgcgcgg
      721 ccgagatcag cgggcccatc tgcgtggact cgtccagcgg gtcgcccacg cgcacctcgc
      781 gcaccgcggc gtccagcagc tcggtgaagc ggtccatggc gtcgcgctgg accaggatgc
      841 gcgagcgcgc gcagcagtcc tggccggcgt tgccgaacac cgacatcggc gcggccgccg
      901 cggcggcctc gaggtcggcg tcggcgaaca cgatgttggc cgacttgccg cccagctcga
      961 gcgtgacccg cttgatggtg tcggcggcct ggcgggcgat gccgcggccg acctcggtcg
     1021 acccggtgaa cgcgaccttg gccacgtcgg ggtgctcgac caaccgcgag ccgcagacgc
     1081 tgccgggccc ggcgaccacg ttcagcacgc cctcgggcag caccgtggcg gcgatcttct
     1141 ccagctccag cgcggtcagc ggcgtcagct ccgcgggctt gagcaccacg gtgttgccgg
     1201 ccgccagcgc cggccccagc ttccaggcgg cgatgaccag cgggaagttc cagggcacga
     1261 tcaggccgac cacgccgagc ggctcgcgga aggtcatgtc gatgccgccg gccaccggga
     1321 tcgtgtcgcc cagcagccgc tcgggcgcgc ccgcgtagta gcggaaggtc tcgaccacca
     1381 tgcccatctc gccgcgggcg tcgccgatgg gcttgccggc gttgcgcgcc tccagcaccg
     1441 ccaggtcctc cagccgctcc tcgaccgcgt cggccagccg gcgcaggacg gccgaccgct
     1501 ccgcgggctc gaccgcccgc caggccggga acgcggcctt cgcgcgctcg actgcggcgt
     1561 ccacgtcgtc agctccggcc cgcgggacct cggccatgac gcccgcggtc gcgggctcga
     1621 ccacggagat cacgcggccg ccttcgcccg ctcggccgcc tcgaccagcg acttgaccac
     1681 gttggcgcgc aggtcctcct cggggtgcca gagcacgccc agcgcgtacg ggcggccggg
     1741 cagctcgatg gcctcgatca cctggtcggg ctccgaccat ccggtgacca cgaggtcctc
     1801 gcccagctcg gccacgccct gatggtggtg cgacttcacc gggatcgacg tgccgcccgc
     1861 cgcctgctcg gccagcgagc cgggctccac gcgcacgtcg tggtcgccga aggtgcccgg
     1921 ggtgtggcga tggtcgtcgt ggcccaggct gtccgggagg tgctggtcga tggtgccgcc
     1981 gcaggccacg ttcagcatct gcatgccgcg gcagatgccc agcaccggca tgtcgcgctc
     2041 cagcgcggcg tgggtcagcg ccagctcgaa gcggtcgcgc tcgggccagc tgcccctggt
     2101 ctgctcgtgc gggcgggcgc cgtagctggc ggggtccacg tcggcgccgc cggccaggat
     2161 cagcgcgtcc acgtggtcca gcagccgggc ggggtcctcc agggcgtggt cgtccggggg
     2221 caggatcagc gccacggcgt cggcgtcctg gaccgcgcgg gcgtagttgc gcggggtcag
     2281 gtgcacctcg gtgtcccagg cgccccagcg gaccttctcg atggccgcgc agatgcccac
     2341 ggcgaccgtc aaagccgttc gaaccctcgg accttctccc aatcggtgac cgccgcgtcg
     2401 aaggccttga gctccacgtc cgcgtggttg aggtagtggt cgacaacctc ctggccgaag
     2461 gcctcccggg ccagttccga gccggcgaac agctcgcgcg cgtcgcgcag ggtcgagggc
     2521 acgcgctcgc ggccggcggt gtaggcgttg ccctccagcg cgggctccag ctccatctcc
     2581 tcgtcgatgc ccttgagccc ggcggcgatc atggccgcca gcgccaggta ggggttcacg
     2641 tcggcgcccg gcaggcggtt ctcgaaccgc agcgagggac cgtggcccac cgcgcggatg
     2701 gcgcacgtgc ggttgtcctc gccccaggcc accgcggtgg gcgcgaacga gccgtcggcg
     2761 aagcgcttgt acgagttgat gtggggcgcg aagaacatgg tcagctcgcg catggcggcc
     2821 agctgcccgg cgacgaagcg ctcgaaggtc ggccggtcct caccgaacac gttggcgccg
     2881 tcctcggagc ccagcgagca gtggatgtgg cacgagttgc cctccagctc gttgaacttg
     2941 gccatgaagg tgatggccat ctcctcctgg tcggcgatct ccttggcgcc gttcttgtaa
     3001 atcgcgtggt cgtcgcagac ccgcagtgcc tcgccgtagt ggaagttgat ctc
//
LOCUS       DASUPW010000186         3189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1153966, whole genome shotgun sequence.
ACCESSION   DASUPW010000186 DASUPW010000000
VERSION     DASUPW010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153966"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFY44_04310"
     CDS             complement(<1..462)
                     /locus_tag="VFY44_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909692.1"
                     /translation="MLGGMMRWAALALAAALALPAMAFAAGFDDPSSGDSSQQLDDGV
                     PVLPYDNLQRDDTPDDPDYDRAEPDDPQGGGSTNVFSERFDLFGFAPARTRTTARYRD
                     PTGPNFGNGMVSGFNAAGAWKITRGDPRVSVAVLDTGVNWDNAGLRIQVRLN"
     gene            complement(502..864)
                     /locus_tag="VFY44_04315"
     CDS             complement(502..864)
                     /locus_tag="VFY44_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909693.1"
                     /translation="MARVALLCPDLLFGSKVEGGLAVAGHTVNRFDDEASIRTAAQFH
                     DLLVVDLTTDLVDGPALVTSMRERGELEAVPTLGFYSHVDVETRRRAQDAGFSRVVPR
                     SRMAREMAALVDGLLPPA"
     gene            complement(875..1237)
                     /locus_tag="VFY44_04320"
     CDS             complement(875..1237)
                     /locus_tag="VFY44_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909694.1"
                     /translation="MALAEDFQRVVDSLPPDWTQVELDLRLLDEDRYIDAATMLTQIN
                     AMPYSENEWHFRLRAANRWGHAAAHQAVAGTLALLDDQGIEGRLVAREARSGRVPVRQ
                     LWGRPESVRQELRRRWAQ"
     gene            complement(1248..2561)
                     /locus_tag="VFY44_04325"
     CDS             complement(1248..2561)
                     /locus_tag="VFY44_04325"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX5909695.1"
                     /translation="MAIDLIFALLLVVANGVFVATEFSVARLRPTQVNELVRERRPGA
                     KSAAHAVEHIDSYLSACQLGITLSSLGLGALGEPAFHDLIEPVLGDGAEVAGFGLATI
                     VAFSVITTLHVVLGELAPKSAAISRTVSVALVMAPIMRVFYMATRPLVDLFNGMGNLV
                     LKPFGIPPASEAGSQPHSEDELRELLRESSQGGLIEADEQQLSDAALVFGDVRAREAM
                     IPRADIDDVQLDYDVHTVARVAIETGRTRLPVHPPEGGLEDAVGVINAKDLLPLLIGE
                     DREADISGLVRPLAHVSESARVDEVLREMRADRLHLALVHDEHGTVTGLITMEDILEE
                     LVGEITDEFDDDEEDAFRPEGDRLIVDGSAPVRLLAERLETEIEGPHESTIGGYLSEE
                     LARVPDEGETVALLGRNARILEVEGTRIVSLAVLAPEPEDDEAQR"
     gene            complement(2585..>3189)
                     /locus_tag="VFY44_04330"
     CDS             complement(2585..>3189)
                     /locus_tag="VFY44_04330"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX5909696.1"
                     /translation="TGKPVATSALAERLAVSPASALAMLKRLVSLGLVTHQPYRGTEL
                     TEAGERVALEVIRHHRLLEAYLAEALGMSWDRVHAEAEVLEHAISPELSELIAEKLGH
                     PTHDPHGDPIPTLDGAIDEQDTVPLAELEPGQSGVFTRISDSDPEMLRYLSQRGIAPG
                     DALEVLERQPFDGPLTVRFGAREHSLGGTLARVMRVAVGR"
BASE COUNT          432 a         1162 c         1167 g          428 t
ORIGIN      
        1 gttcaggcgc acctggatgc gcaggccggc gttgtcccag ttcacgccgg tgtccagcac
       61 cgccaccgag acgcgcgggt cgccgcgggt gatcttccag gcgcccgcgg cgttgaagcc
      121 cgagaccatc ccgttgccga agttcggtcc cgtggggtcg cggtagcgcg cggtggtgcg
      181 ggtccgcgcc ggcgcgaagc cgaacaggtc gaagcgctcg gagaagacgt tggtggaccc
      241 gccgccctgg gggtcgtcgg gctcggcccg gtcgtagtcg gggtcgtcgg gcgtgtcgtc
      301 gcgctggagg ttgtcgtagg gcagcacggg caccccgtcg tcgagctgct gcgacgagtc
      361 gccggacgac gggtcgtcga acccggccgc gaacgccatc gcgggcagcg caagggccgc
      421 cgccagggcg agcgccgccc atcgcatcat gcctcccagc atgcggccac cctcccacag
      481 ggccggccgc cgtgcgccgg ttcaggccgg gggcagcagc ccgtccacga gcgcggccat
      541 ctcgcgcgcc atccgcgacc gcgggaccac gcgcgagaac ccggcgtcct gggcgcggcg
      601 gcgggtctcg acgtccacgt gggagtagaa gcccagcgtg ggcacggcct ccagctcgcc
      661 gcgctcgcgc atcgaggtca ccagcgccgg gccgtcgacc aagtcggtgg tcaggtccac
      721 caccagcagg tcgtggaact gggcggcggt gcggatcgac gcctcgtcgt cgaagcggtt
      781 gacggtgtgg cccgccacgg ccaggccgcc ctcgaccttg gatccgaaca acaggtccgg
      841 gcagagcagg gcgacgcggg ccaccccggc cccgctactg cgcccagcgg cggcgaagct
      901 cctggcggac cgactcgggg cggccccaga gctggcgtac cgggacgcgg cccgagcggg
      961 cctcgcgggc caccagccgg ccctcgatgc cctggtcgtc cagcagcgcc agcgtgccgg
     1021 ccaccgcctg gtgggcggcc gcgtggcccc agcggttggc cgcccgcagg cggaagtgcc
     1081 actcgttctc ggagtagggc atcgcgttga tctgcgtgag catcgtcgcg gcgtcgatgt
     1141 agcggtcctc gtccagcagg cgcaggtcca gctcgacctg ggtccagtcc ggcggcaggc
     1201 tgtccacgac gcgctggaag tcttcggcta gggccacggg cgcaagccta ccgctgagcc
     1261 tcgtcgtcct cgggctcggg cgccagcacg gccaggctga cgatgcgggt gccctcgacc
     1321 tcgaggatgc gggcgttgcg gcccaggagc gccaccgtct ccccctcgtc cggcacgcgc
     1381 gccagctcct ccgacaggta gccgccgatc gtgctctcgt gcgggccctc gatctcggtc
     1441 tccagccgct cggccagaag ccgcacggga gcgctgccgt ccacgatcag ccggtcgccc
     1501 tcggggcgga acgcgtcctc ctcgtcgtcg tcgaactcgt ccgtgatctc gccgaccagc
     1561 tcctcgagga tgtcctccat cgtgatcagg ccggtcacgg tgccgtgctc gtcgtggacc
     1621 agcgccaggt gcaggcggtc ggcgcgcatc tcgcgcagca cctcgtccac gcgcgcggac
     1681 tcggagacgt gcgcaagcgg gcggaccagc ccgctgatgt ccgcctcgcg gtcttcgccg
     1741 atcagcagcg gcagcaggtc cttggcgttg atcaccccga ccgcgtcctc cagcccgccc
     1801 tcgggcgggt gcacgggaag ccgcgtgcgg ccggtctcga tggccacgcg ggccacggtg
     1861 tgcacgtcgt agtccagctg cacgtcgtcg atgtcggcgc gggggatcat cgcctcgcgc
     1921 gcccggacgt cgccgaacac cagcgccgcg tccgagagct gctgctcgtc ggcctcgatc
     1981 agcccgccct gcgagctctc gcgcagcagc tcgcgcagct cgtcctcgct gtggggctgg
     2041 ctgccggcct cggaagcggg cgggatcccg aacggcttga gcaccaggtt gcccatgccg
     2101 ttgaacaggt ccaccagcgg ccgggtggcc atgtagaaga cgcgcatgat cggcgccatc
     2161 accagcgcca ccgacacggt gcgcgagatg gcggcgctct tgggggccag ctcgcccagc
     2221 accacgtgca gcgtggtgat caccgagaac gccacgatcg tggccaggcc gaagcccgcc
     2281 acctcggcgc cgtcgcccag gaccggctcg atcaggtcgt ggaaggcggg ctcgcccagg
     2341 gcgcccaggc ccagcgacga gagcgtgatg cccagctggc aggccgacag gtaggagtcg
     2401 atgtgctcga cggcgtgggc cgcggacttg gcccccggcc ggcgctctcg gaccagctcg
     2461 ttcacctgtg tgggccgcag ccgggccacg gagaactcgg tggccacgaa cacgccgttg
     2521 gccaccacca gcagcagggc gaagatcagg tcgatggcca cgtgcgcagg acgttagccg
     2581 tcgctcaacg gccgacggcc acacgcatga cgcgcgcgag ggtgccgccc agggagtgct
     2641 cgcgcgcgcc gaagcggacc gtcagcggcc cgtcgaacgg ctggcgctcc agcacctcca
     2701 gcgcatcgcc gggggcgatg ccgcgctgtg acaggtagcg cagcatctcg ggatccgagt
     2761 ccgagatgcg ggtgaacacc ccggactggc cgggctcgag ctcggccagc ggcaccgtgt
     2821 cctgctcgtc gatggccccg tccagggtgg ggatcgggtc cccgtggggg tcgtgcgtcg
     2881 ggtggccgag cttctcggcg atcagctcgg acagctcggg cgagatggcg tgctcgagca
     2941 cctcggcctc ggcgtgcacg cggtcccagg acatgcccag cgcttcagcc aggtaggcct
     3001 ccagtaagcg gtggtggcgg atgacctcga gcgccacccg ctcgcccgcc tcggtcagct
     3061 cggtgccgcg gtagggctgg tgggtcacca ggcccagcga gaccaggcgc ttgagcatcg
     3121 ccaacgccga cgcgggcgac accgccaggc gctcggccag cgccgaggtg gccacgggct
     3181 tgccggtgc
//
LOCUS       DASUPW010000187         5488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_599482, whole genome shotgun sequence.
ACCESSION   DASUPW010000187 DASUPW010000000
VERSION     DASUPW010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599482"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="VFY44_04335"
     CDS             <1..874
                     /locus_tag="VFY44_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909697.1"
                     /translation="PGAPLRPVGHGGAMRLHRLLPVPVCLLALAAPAAAKPVKYQLSV
                     QGTQVTTWDYVKRQAPTCDWPESESGRQAIEFGTGAGKTTITMSASRKGALTLAPGMV
                     SLPAKATLQRHYRRMFAQQSPCPGGGPYGGGEGPPRNAVGTAKCNVTGKVDFRMGGTV
                     QSVFDTVDPMRPPTALRAPAGFLIARADPHWGEALGSSERSLPARCSATGQNDADIAI
                     TDGEREWSGSVIESKSWLALKKVRKLRRGKTLKFSLKRRKSYPSSTHPTPGNPDTKGK
                     TVLDVDVALKRISR"
     gene            complement(864..2330)
                     /locus_tag="VFY44_04340"
     CDS             complement(864..2330)
                     /locus_tag="VFY44_04340"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX5909698.1"
                     /translation="MSILRTKSVEQSIADTEEGEHQLDKALGPIDLTVIGVGVIIGTG
                     IFVLTGEAAANLAGPSVAISFVVAGVVCALAALCYAEFASTVPVAGSAYTFAYASLGE
                     LFAWIIGWDLILELALGSATVASGWSDYLRVVMDETFGVNLPAFIDGEGHNLAAAGIV
                     LVVTALLCVGIKVSNRVNAVVVAIKVTVVLLVIIAGFFYIKGGNYSPFVPPTGTEPQA
                     ASDPSLLEEIGLGTGSFGIGGIFTAAALVFFAYIGFDVVATAAEETKKPQRDMPIGIF
                     GSLAICTTLYVLVSLVVTGMVSFDKIEVEAPLAAAFQSVGKDGIAQIISFGALAGLFT
                     VMLILMLGQSRVFFAMSRDKLLPPFFSHVSPRFKTPYRSTLATGAVVALMAGLIPLST
                     LAQLVNIGTLFAFMVVAAGVIILRRNQPDLERAFRAPLVPFLPIVSILASFYLTLNLP
                     AETWERFLIWMALGFVIYFVYARHKSKLATEGETAASG"
     gene            complement(2340..3605)
                     /locus_tag="VFY44_04345"
     CDS             complement(2340..3605)
                     /locus_tag="VFY44_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C
                     domain-containing protein"
                     /protein_id="HEX5909699.1"
                     /translation="MRGVRIAGMVIAAWLAALVAAPTAGAADDRRQTLRQLDLQYELA
                     GDVPMRNVPWVFTHNSYNSPAEMGLALSPQDSNQRITVVDQLDEGVRSLELDVHLFLG
                     RPVLCHSTGPHLGCTTEKTFRQVLTQIRAWMNRNPRQVLLLYLEGHLDDAAGYDLGSA
                     DLDAVVGDLVYRPPSRGARCDPLPLELTRNQVRAAGKRLVLMGDCGDGARWPAYVHDD
                     RQRLTASTNAPFRDFPDCGPDYTRAEYESRQVRYYEDATGVGAATGSGHGRIAPPLVG
                     RMTRCGVDLIGFDLLERGDARVAAQVWSWGPGEPARGHDCAIQGAGGRWFARPCGERH
                     RVACRDSTGLWRIARKATARGAARACGRPGLLQAVPRTGYEGQRLLSAQKRAGSGAVW
                     LGIRRRGDGWKAYERKGCRRRALARRCPG"
     gene            complement(3622..4362)
                     /locus_tag="VFY44_04350"
     CDS             complement(3622..4362)
                     /locus_tag="VFY44_04350"
                     /inference="COORDINATES: protein motif:HMM:NF018496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HEX5909700.1"
                     /translation="MVNRVDVGSVLSRAFAIYKDQAAVLLPVAFGLFLIVGVLQGLLG
                     GLLVLLATVASLIASTLYTGMVVKLVDDVRDGRRDNSAGDLLGSAAPVIAPLILAGLV
                     VGLLVVIGFILLIVPGLILLTIFAVVAPVIVIERAGVFEALGRSRALVKGNGWNVFGV
                     IIVAFLISFVVSAVLGGIGAAIGGVVAHLIFSVIANTLTAPIGALVAAVLYFTLRGDT
                     AAPAPGANVATTPDTSATPPPPPPPPAQ"
     gene            complement(4417..>5488)
                     /locus_tag="VFY44_04355"
     CDS             complement(4417..>5488)
                     /locus_tag="VFY44_04355"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5909701.1"
                     /translation="ATTFTDRAERNKALGLWGAMGGVGGATGALLGGALTQTLNWRWV
                     LFINLPIGLFATFAAWRVVAEGRAERPGRHFDVAGAVSVTAGLVVLTYGIVRTEVNGW
                     ASATTLVTLGAGLVLLVAFVFIEARLAREPLMPLRIFRSLPLSAANLVVFALGSSVFA
                     MWYFVTLYLQNVLGYSPLRAGFAFLPMTVLIVICSRFAGPLVSRRGPWGMLAAGMSMI
                     AVGLALFSQVSPDGTYLGDVLAPSLLCAAGIGFSFVPVTIAATSGVDPSESGLASGLV
                     NTSRQVGGSLGLAVLATIATTRTADLAGVTSAAEAATAGFDRAFTVGAVFAVAGALAA
                     LFAARTSHNRRRRAEPAPATPA"
BASE COUNT          878 a         1964 c         1998 g          648 t
ORIGIN      
        1 ccccggtgcg ccgctgcggc ccgtggggca cggtggcgcc atgcgactcc accgactgct
       61 ccccgttccc gtctgcctgc tggcgctggc cgctcccgcg gctgcgaagc cggtgaagta
      121 ccagctctcc gtgcagggca cccaggtcac cacctgggac tacgtgaagc gccaggcgcc
      181 cacgtgcgac tggcccgagt ccgagagcgg gcgccaggcg atcgagttcg gcaccggtgc
      241 cggcaagacc acgatcacca tgagcgcgag ccgcaagggc gcgctgacgc tggcgccggg
      301 catggtcagc ctgcccgcca aggccacgct gcagcgccac taccggcgca tgttcgccca
      361 gcagtcaccg tgccccgggg gcggccccta cggtggcggc gagggcccgc ccaggaacgc
      421 ggtcggcacc gccaagtgca acgtgaccgg caaggtcgac ttccgcatgg gcggcacggt
      481 gcagtcggtg ttcgacacgg tcgacccgat gcgaccgccg accgccctgc gcgccccggc
      541 cgggttcctg atcgcccggg ccgacccgca ctggggcgag gccctgggca gcagcgagcg
      601 ctcgttgccc gcgcgttgct cggccacggg ccagaacgac gccgacatcg ccatcaccga
      661 cggcgagcgc gagtggtcgg gctcggtgat cgagtccaag tcctggctgg cgctgaagaa
      721 ggtcaggaag ctgcgccgcg gcaagacgct gaagttctcg ctcaagcgca ggaagtccta
      781 ccccagctcg acgcacccga ccccgggcaa cccggacacg aagggcaaga cggtgctgga
      841 cgtggacgtg gcgctgaagc ggatcagccg ctagcggccg tctcgccctc ggtggccagc
      901 ttgctcttgt ggcgggcgta gacgaagtag atgacgaacc cgagcgccat ccagatcagg
      961 aaccgctccc aggtctcggc cggcaggttc agcgtcaggt agaacgacgc caggatcgag
     1021 acgatcggca ggaacgggac cagcggggcg cggaaggcgc gctcgaggtc gggctggttg
     1081 cgccggagga tgatcacccc ggcggcgacc accatgaagg cgaacagggt gccgatgttc
     1141 accagctgcg ccagcgtgct cagggggatc agcccggcca tgagcgccac cacggcgccg
     1201 gtggccaggg tggagcggta cggcgtcttg aaccgcggcg agacgtgtga gaagaacggc
     1261 ggcagcagct tgtcgcggct catggcgaag aacacccgcg actggccgag catcaggatc
     1321 agcatgaccg tgaacaggcc cgccagcgcc ccgaacgaga tgatctgcgc gatcccgtcc
     1381 ttgcccaccg actggaacgc ggcggccagc ggggcctcga cctcgatctt gtcgaacgag
     1441 accatgccgg tgaccacgag cgagaccagc acgtacagcg tggtgcagat ggccagcgac
     1501 ccgaagatgc cgatgggcat gtcgcgctgg ggcttcttgg tctcctccgc ggcggtggcc
     1561 accacgtcga agccgatgta ggcgaagaac accagcgcgg cggcggtgaa gatgccgccg
     1621 atcccgaacg agcccgtgcc caggccgatc tcctccagca ggctcgggtc ggaggccgcc
     1681 tggggctcgg tgccggtggg gggcacgaac ggcgagtagt tgccgccctt gatgtagaag
     1741 aacccggcga tgatcaccag caggacgacg gtgaccttga tggccaccac cacggcgttc
     1801 acgcggttgg agaccttgat gcccacgcac agcagcgccg tgaccacgag cacgatcccg
     1861 gcggcggcca ggttgtggcc ctcgccgtcg atgaacgccg gcaggttcac gccgaacgtc
     1921 tcgtccatga ccacgcgcag atagtccgac catcccgacg ccaccgtcgc cgagcccagc
     1981 gccagctcca ggatcaggtc ccagccgatg atccaggcga acagctcgcc caggctggcg
     2041 taggcgaagg tgtaggccga ccccgccacc ggcacggtcg aggcgaactc ggcgtagcac
     2101 agcgccgcga gcgcgcagac caccccggcc acgacgaacg agatggccac cgacggcccg
     2161 gccaggttcg ccgcggcctc gccggtgagc acgaagatgc cggtgccgat gatgaccccg
     2221 accccgatca cggtcaggtc gatcggcccg agcgccttgt ccagctggtg ctcgccctcc
     2281 tccgtgtccg cgatcgactg ttcgacggac ttggttcgga ggatgctcat cgggtcgcct
     2341 taccccgggc agcggcgcgc taacgcacgc cggcgacatc ccttgcgctc gtaggccttc
     2401 cagccgtcgc cgcggcggcg gatgcccagc cacaccgcgc ccgagccggc gcgcttctgc
     2461 gcggacagca gccgctggcc ctcgtagccg gtccggggca cggcctggag caggcccgga
     2521 cgcccgcagg cgcgggcggc gccgcgcgcg gtggccttgc gcgcgatccg ccacaggccg
     2581 gtggagtcgc ggcaggccac gcggtggcgc tcgccgcacg ggcgcgcgaa ccagcgcccg
     2641 ccggcgccct ggatcgcgca gtcgtgcccg cgcgccggct cgcccgggcc ccagctccac
     2701 acctgggcgg ccacgcgggc gtcgccgcgc tccaggaggt cgaagccgat caggtcgacc
     2761 ccgcagcggg tcatccgccc caccagcggc ggagcgatcc ggccgtggcc cgagccggtg
     2821 gcggcgccga cgccggtggc gtcctcgtag tagcgcacct ggcgcgactc gtactcggcg
     2881 cgcgtgtagt cgggcccgca gtccgggaag tcgcggaacg gcgcgttggt ggaggccgtc
     2941 agccgttggc ggtcgtcgtg cacataggcg ggccagcggg cgccgtcgcc gcagtcgccc
     3001 atcagcacca gccgcttgcc cgcggcgcgg acctggttgc gggtgagctc cagcggcagc
     3061 gggtcgcagc gcgcgccccg gctgggcggg cggtagacca ggtcgcccac cacggcgtcc
     3121 aggtcggcgg acccgaggtc gtagcccgcg gcgtcgtcca ggtggccctc caggtacagc
     3181 agcagcacct ggcgcgggtt gcggttcatc caggcccgga tctgggtcag cacctggcgg
     3241 aaggtcttct cggtggtgca gcccaggtgc gggccggtgg agtggcacag caccggccgg
     3301 cccaggaaca ggtgcacgtc cagctccagg ctgcgcaccc cctcgtccag ctggtcgacg
     3361 acggtgatcc tctggttgct gtcctggggc gacagcgcca gccccatctc ggccgggctg
     3421 ttgtacgagt tgtgggtgaa cacccacggg acgttgcgca tgggcacgtc gccggccagc
     3481 tcgtactgca ggtccagctg gcgaagggtc tgcctgcggt cgtccgccgc ccctgccgtg
     3541 ggcgcggcca ccagggcagc cagccaggca gcgatgacca tccccgcgat ccggacaccc
     3601 cgcacgcggg gcagcgtagc cctactgggc gggcggaggc ggcggtgggg gaggcgtggc
     3661 cgaggtgtcc ggcgtggtcg ccacgttggc gcccggggcc ggcgcggccg tgtccccgcg
     3721 cagcgtgaag tacaggaccg cggccacgag cgcgccgatg ggcgcggtca gggtgttggc
     3781 gatcacgctg aagatgaggt gggccaccac gccgccgatg gcggcgccga tgccgccgag
     3841 caccgcgctg accacgaacg agatcaggaa ggcgacgatg atgacgccga acacgttcca
     3901 gccgttgccc ttgaccagcg cccgggagcg gcccagggcc tcgaagacgc cggcgcgctc
     3961 gatcacgatg accggcgcga ccacggcgaa gatggtcagc aggatcagcc cggggacgat
     4021 cagcaggatg aagccgatca ccacgagcag gccgacgacc aggccggcca ggatcagcgg
     4081 tgcgatgacc ggggccgccg agcccaggag gtcgcccgcg gagttgtcgc ggcgaccgtc
     4141 gcggacgtcg tcgacgagct tgacgaccat gccggtgtac agcgtgctgg cgatcaggct
     4201 ggcgaccgtg gccaggagca ccaggagacc gcccaggagg ccctggagca cgcccacgat
     4261 gaggaacagg ccgaaggcca ccgggagcag caccgccgcc tggtccttgt agatggcgaa
     4321 tgccctgctg agaacgctgc cgacgtcgac tctgttgacc atgtgcgcca acataccctg
     4381 ggccccggtc gtcagcggcg aacccggacg aacggtctag gcgggggtgg ccggcgccgg
     4441 ctcggctctg cgccgccggt tgtggctggt gcgggcggcg aacagcgcgg ccagcgcgcc
     4501 cgccaccgcg aacaccgcgc ccaccgtgaa ggcgcggtcg aagccggccg tggcggcctc
     4561 cgccgcgctg gtgacgccgg ccaggtcggc ggtgcgcgtg gtggcgatgg tggccaggac
     4621 cgccaggcca agcgacccgc ccacctggcg tgaggtgttc accagccccg aggccaggcc
     4681 cgactcggac gggtcgacgc cgctggtggc ggcgatggtc accggcacga acgagaagcc
     4741 gatcccggcc gcgcacagga gcgacggcgc caggacgtcg ccgaggtagg tgccgtccgg
     4801 gctgacctgg ctgaacagcg ccagcccgac cgcgatcatc gacatgcccg cggccagcat
     4861 gccccacggg ccgcggcgcg agaccagcgg cccggcgaag cgcgagcaga tcacgatcag
     4921 cacggtcatg ggcaggaacg cgaaccccgc gcgcaggggc gagtagccga ggacgttctg
     4981 caggtagagc gtcacgaagt accacatggc gaacaccgag ctgccgagcg cgaagaccac
     5041 caggttggcc gcgctcagcg gcagcgagcg gaagatccgc agcggcatca gcggctcgcg
     5101 cgccagccgc gcctcgatga acacgaacgc caccagcagc acgagcccgg cgcccagcgt
     5161 gaccagcgtc gtcgccgagg cccacccgtt gacctcggtg cgcacgatgc cgtaggtcag
     5221 caccaccagc ccggcggtca ccgagaccgc gccggccacg tcgaagtggc gcccggggcg
     5281 ctcggcgcgc ccctcggcca ccacgcgcca ggcggcgaag gtggcgaaca ggccgatcgg
     5341 caggttgatg aacagcaccc agcgccagtt cagcgtctgc gtcagcgcgc cgcccagcag
     5401 cgcgccggtg gcgccgccga cgccgcccat cgcgccccac agccccagcg ccttgttgcg
     5461 ctcggcgcgg tcggtgaacg tggtggcc
//
LOCUS       DASUPW010000188         2379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1708094, whole genome shotgun sequence.
ACCESSION   DASUPW010000188 DASUPW010000000
VERSION     DASUPW010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1708094"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VFY44_04360"
     CDS             <1..250
                     /locus_tag="VFY44_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909702.1"
                     /translation="MRREEPWGSSRAGWVPGEQSSPVGPAFQGVKWDDPSAFDRAARR
                     CGTGPPPQPERSAVAAGMFGVLVAALFMAYRRTRIARR"
     gene            247..531
                     /gene="gatC"
                     /locus_tag="VFY44_04365"
     CDS             247..531
                     /gene="gatC"
                     /locus_tag="VFY44_04365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX5909703.1"
                     /translation="MIDREQVLYVARLARLRLSEEEIERMTGELSSILGHIDTISGLD
                     LDGVEPTARVVDVENVLRPDEPRPSLPREAVLEQAPDTDGEGFRVPSPGA"
     gene            528..1940
                     /gene="gatA"
                     /locus_tag="VFY44_04370"
     CDS             528..1940
                     /gene="gatA"
                     /locus_tag="VFY44_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX5909704.1"
                     /translation="MSDLLDLTGAQAAERIAAGEVSAPELFDAYRDRAGADELNAYLW
                     VADGAPNVADGPLAGVPLAVKDLFCTEGVPSQAASRILEGYRPPYTATAVRRLTDAGA
                     ALLGKTNMDEFAMGSSNENSGFGPVRNPWDTAKVPGGSSGGSAAAVAAGSAPWAIGTD
                     TGGSIRQPASLCGIVGMKPTYGAVSRYGMIAFASSLDQAGPLTRDVTDAALLLRHMVG
                     ADPCDSTSTGVPDPIELPTAERLDGLRFGVPPQLTGGGIDPGVSEVFDATLRRIEELG
                     GTLTEIDLPHAEHAIAAYYVIAPAEASANLARFDGVRYGRRADGVTDLLSLYEETREQ
                     GFGDEVKRRIMIGTYALSSGYYDAYYGSAQKVRTKILDDFRAAYEQVDLIVTPAAPTT
                     AFDIGELAGDPLAMYQQDFCTVPFSLAGLPGISIPAGLAGGLPVGFQLAGPAFSENRI
                     LDAAYALEQAIGFEPGGARG"
     gene            1933..>2379
                     /locus_tag="VFY44_04375"
     CDS             1933..>2379
                     /locus_tag="VFY44_04375"
                     /inference="COORDINATES: protein motif:HMM:NF014930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HEX5909705.1"
                     /translation="MAEWEPVIGLEIHVQLQTRTKMFCGCALSFGEEPNTRTCPVCLG
                     LPGALPVANAEAVHYALMIGMALGCQIAPRSIFHRKNYFYPDSPKAYQISQYDIPVCG
                     PGRLGDIRIHRAHLEEDAAKLVHAGASGRIHSAESSVVDFNRCGTPL"
BASE COUNT          333 a          882 c          850 g          314 t
ORIGIN      
        1 gatgcgccgg gaggagccct ggggctcgtc ccgggcgggg tgggtgccgg gggagcagtc
       61 gagccccgtc ggcccggcgt tccagggcgt caagtgggac gacccgtccg ccttcgaccg
      121 cgcggccagg agatgcggca cggggccgcc gccccagccg gagcgcagcg cggtggcggc
      181 cggcatgttc ggcgtcctgg tggccgcgct cttcatggcg tatcgcagga ctagaatcgc
      241 gcgccgatga tcgaccgcga acaggtcctg tacgtagccc gcctggcccg cctgcgtctc
      301 tccgaggagg agatcgagcg gatgaccggc gagctgagct cgatcctggg ccacatcgac
      361 accatctcgg ggctggacct ggacggggtg gagcccaccg cacgggtcgt ggacgtcgag
      421 aacgtcctgc ggcccgacga gccgcggccc agcctgccgc gcgaggccgt cctggagcag
      481 gcgcccgaca ccgacggcga gggcttccgc gtgcccagcc ccggcgcatg agcgacctgc
      541 tcgacctgac cggcgcccag gccgccgagc ggatcgccgc cggcgaggtc tcggcgccgg
      601 agctgttcga cgcctaccgc gaccgcgccg gggccgacga gctgaacgcc tacctgtggg
      661 tggccgacgg cgcgcccaac gtggccgacg ggccgctggc cggcgtgccg ctggcggtca
      721 aggacctgtt ctgcaccgag ggcgttccca gccaggccgc ctcgcgcatc ctggagggct
      781 accggccgcc gtacaccgcg accgcggtgc gccgcctgac cgacgccggc gcggccctgc
      841 tgggcaagac gaacatggac gagttcgcca tgggctcgtc caacgagaac tccggcttcg
      901 gcccggtcag gaacccgtgg gacacggcca aggtgccggg gggatcgtcc ggcggcagcg
      961 ccgcggcggt ggccgcaggt tcggctccgt gggccatcgg caccgacacc ggcgggtcca
     1021 tccgccagcc cgcgtcgctg tgcgggatcg tgggcatgaa gcccacctac ggcgccgtgt
     1081 cgcgttacgg gatgatcgcc ttcgcctcgt cgctggacca ggcggggccc ctgacccggg
     1141 acgtcaccga cgccgcgctg ctgctgcgcc acatggtcgg cgccgacccg tgcgactcca
     1201 cctccacggg cgtgcccgac ccgatcgagc tgcccaccgc ggaacgcctg gacggcctgc
     1261 gcttcggcgt gccgccccag ctgaccggcg gcggcatcga ccccggcgtg tccgaggtct
     1321 tcgacgccac gctgaggcgg atcgaggagc tcggcggaac cctcaccgag atcgacctgc
     1381 cgcacgccga gcacgcgatc gccgcctact acgtgatcgc gccggccgag gccagcgcca
     1441 acctggcccg cttcgacggc gtgcgctacg gccgccgcgc cgacggcgtc accgacctgc
     1501 tgtcgctgta cgaggagacc cgtgagcagg gcttcggcga cgaggtcaag cgccggatca
     1561 tgatcggcac ctacgcgctg tcctcgggct actacgacgc ctactacggc agcgcccaga
     1621 aggtgcgcac caagatcctg gacgacttcc gcgcggccta cgagcaggtc gacctgatcg
     1681 tcaccccggc cgcgcccacc accgccttcg acatcggcga gctggccggc gacccgctgg
     1741 ccatgtacca gcaggacttc tgcaccgtgc ccttctcgct cgccgggctg ccgggcatct
     1801 cgatccccgc cggcctggcc ggggggctgc cggtgggctt ccagctggcg ggccccgcgt
     1861 tcagcgagaa ccggatcctg gacgccgcgt acgcgctgga gcaggccatc ggcttcgaac
     1921 cggggggcgc tcgtggctga gtgggaaccc gtcatcgggc tggagatcca cgtccagctg
     1981 cagacgcgga ccaagatgtt ctgcggctgc gcgctgtcgt tcggcgagga gcccaacacc
     2041 cgaacgtgcc cggtctgcct gggactgccc ggcgcgctgc cggtggccaa cgccgaggcg
     2101 gtgcactacg cgctgatgat cgggatggcg ctgggctgcc agatcgcgcc gcggtcgatc
     2161 ttccaccgca agaactactt ctatcccgac tcgcccaagg cgtaccagat cagccagtac
     2221 gacatcccgg tctgcgggcc gggacggctg ggcgacatcc ggatccaccg cgcccacctg
     2281 gaggaggacg cggccaagct ggtgcacgcg ggcgcgtccg gccggatcca ctcagccgag
     2341 tccagcgtgg tcgacttcaa ccgctgcggc acgccgctg
//
LOCUS       DASUPW010000189         2122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_46736, whole genome shotgun sequence.
ACCESSION   DASUPW010000189 DASUPW010000000
VERSION     DASUPW010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46736"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VFY44_04380"
     CDS             <1..624
                     /locus_tag="VFY44_04380"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX5909706.1"
                     /translation="PGFWTVACDYATGRRVAFGRPGAPDADLADAVAASCAIPGFYRP
                     VDIGGRRYVDGGVRSASNLDLLAGHGLDLVICLNPTSTRVAGGAGLRGVNPLDRVMGL
                     NRAASGRRLGSEAKRVRAGGSEVVLIQPTSEDLAVIGNNLMSGKRRNQIIDTARRTVA
                     RALNLPGIREHLSGLPAGEPHKITRPPGPPDQWPEIVPAPASPARAA"
     gene            827..1657
                     /locus_tag="VFY44_04385"
     CDS             827..1657
                     /locus_tag="VFY44_04385"
                     /inference="COORDINATES: protein motif:HMM:NF015915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5909707.1"
                     /translation="MGEITKQIQARVPKADLDERDPDYIRDTLPRLWLLASLWFRGEV
                     RGLERIPAEGPVLLVGNHSGGNVTPDTGVFTLAFNTYFGVERRFYQLAHNLVLSWPGL
                     GFLRKYGTVAASPANADQALASGAALLVYPGGDYEVHRPSWDAAKVDFDGRKGFLRLA
                     LQHDVPIVPVVSVGGQETALFLSRGEGLAKALRLDKIARLKVLPISLALPWGLNVGDM
                     LGHLPAPAKITIQVLKPIHLREQYGKSPDIDRIYADVIDQMQDTLNALQDERTFPVIG
                     "
     gene            1667..>2122
                     /locus_tag="VFY44_04390"
     CDS             1667..>2122
                     /locus_tag="VFY44_04390"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5909708.1"
                     /translation="MRVEEHVDVGARRKDVWEKLSDTSEWPRLIDNVRQMQPDGESED
                     HAQLGSRYRMRMHVGSADVGGLVEVVELDEGRDMAWTSVTGIDQRGRWRLRDLEDGGT
                     RVTLRLSYGAPGGVVGALTEQIAVGQVRTNVRRGLARLKADIDGVDAEVE"
BASE COUNT          307 a          763 c          792 g          260 t
ORIGIN      
        1 cccggcttct ggaccgtggc ctgcgactac gccaccggcc gccgcgtcgc cttcggccgc
       61 cccggcgcgc ccgacgccga cctggcggac gcggtcgcgg cgtcgtgcgc catccccggc
      121 ttctaccggc cggtcgacat cggcggccgc cgctacgtgg acggcggcgt gcgctcggcc
      181 tcgaatctgg acctcctggc gggccacggc ctggacctgg tgatctgcct gaacccgacc
      241 tccacccggg tggccggcgg cgccgggctg aggggcgtga acccgctgga ccgcgtgatg
      301 ggcctgaacc gcgccgcgtc gggccggcgc ctgggcagcg aggccaagcg cgtccgcgcc
      361 ggcggcagcg aggtggtgct gatccagccg accagcgagg acctggcggt gatcggcaac
      421 aacctgatgt ccggcaagcg ccgcaaccag atcatcgaca cggcgcggcg caccgtggcc
      481 cgggccctga acctgccggg catccgggag cacctgtccg gcctcccggc cggcgagccg
      541 cacaagatca cccggccgcc cggcccgccc gaccagtggc cggagatcgt gcccgcgccc
      601 gcgtcgcccg cccgcgcggc ctgaccaccc gggcgccgca ccgcgcccgc cgtagtggca
      661 gactcccgag tcgatggcgg cgggatcgag aggaacgagc gacaggggtc gctcgtcgcg
      721 gaacggtgcg tcccggaacg ggacatcgtc caatggcgca tcgagccatg acgaaccggc
      781 gaaggccccg gggctcggcg acgcgcaggc cctgatcggc cgtgcgatgg gggagatcac
      841 caagcagatc caggcgcgcg tgcccaaggc cgacctggac gagcgcgacc ccgactacat
      901 ccgcgacacg ctgccgcggc tgtggctgct ggccagcctc tggttccgcg gcgaggtgcg
      961 gggcctggag cggatcccgg ccgagggccc ggtgctgctg gtgggcaacc actcgggcgg
     1021 caacgtgacc cccgacaccg gcgtgttcac gctggccttc aacacctact tcggcgtcga
     1081 gcggcgcttc taccagctgg cccacaacct ggtcctgtcc tggcccggcc tgggcttcct
     1141 gcgcaagtac ggcaccgtgg cggcgtcgcc ggccaacgcc gaccaggcgc tggcctcggg
     1201 cgcggcgctg ctggtctacc cgggcggcga ctacgaggtc caccgcccct cctgggacgc
     1261 ggccaaggtc gacttcgacg gccgcaaggg cttcctgcgc ctggcgctcc agcacgacgt
     1321 gccgatcgtg ccggtggtct ccgtgggggg ccaggagacc gcgctcttcc tcagccgcgg
     1381 cgagggcctg gccaaggcgc tgcgcctgga caagatcgcc cggctgaagg tgctgccgat
     1441 ctcgctggcg ctgccctggg gcctgaacgt gggcgacatg ctcggccacc tgcccgcgcc
     1501 ggcgaagatc acgatccagg tcctgaagcc gatccacctg cgcgagcagt acggcaagag
     1561 cccggacatc gaccggatct acgccgacgt gatcgaccag atgcaggaca cgctgaacgc
     1621 gctccaggac gagcgcacgt tcccggtgat cgggtaaggg gccggcatgc gcgtcgagga
     1681 acacgtggac gtcggggccc gccgcaagga cgtgtgggag aagctgtccg acacctcgga
     1741 gtggccgcgc ctgatcgaca acgtccgcca gatgcagccc gacggcgaga gcgaggacca
     1801 cgcccagctg gggtcgcgct accggatgcg gatgcacgtg ggctcggccg acgtgggcgg
     1861 gctggtggag gtcgtggaac tggacgaggg ccgcgacatg gcctggacca gcgtgaccgg
     1921 catagaccaa cgcgggcgct ggcgcctgcg cgacctggag gacggtggga cgcgggtcac
     1981 cctgcggctg agctacggcg cccccggcgg ggtcgtgggc gcgctgaccg agcagatcgc
     2041 ggtcgggcag gtgcggacga acgtgcggcg tgggctcgcg cggctgaaag cggacatcga
     2101 cggcgttgac gccgaagtgg ag
//
LOCUS       DASUPW010000190         3314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1848532, whole genome shotgun sequence.
ACCESSION   DASUPW010000190 DASUPW010000000
VERSION     DASUPW010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1848532"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VFY44_04395"
     CDS             complement(<1..416)
                     /locus_tag="VFY44_04395"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5909709.1"
                     /translation="MGNAPLGDLVVLDFSRVLAAPLATMLLADHGATVLKVERPGRGD
                     DTRGWGPPHAPDGTATYFLSVNRGKHSVALDLADDADLAVARELAEGADVVVQNFRPG
                     VMERLGLDHETLSAANPRLISCTVTGFGSGAGAAMPG"
     gene            463..1302
                     /locus_tag="VFY44_04400"
     CDS             463..1302
                     /locus_tag="VFY44_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5909710.1"
                     /translation="MPTDTTVPAVTLNDGVAIPQLGFGVFQIPPGDTVEATLRAFQTG
                     YRHIDTAAAYQNEAEVGQAFRASGLDRDEVFITTKCFNDSHGYEQAKQAFQSSIDRLE
                     LEFVDLYLIHWPVPSQDKYVETWKAFIELKEQGLIRSIGVSNFQPPHLRRIIDETGVT
                     PSVNQVELHPRFQQGGLRREHEDLGVVTEAWSPLAQGAVLDDPVLGAIAEEHGKTTGQ
                     VVLRWHVQLGNVVFPKSVTPERIEENFGIFDFHLSEDEMGRIEALDAGERQGPDPDTF
                     VRP"
     gene            complement(1299..1781)
                     /locus_tag="VFY44_04405"
     CDS             complement(1299..1781)
                     /locus_tag="VFY44_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909711.1"
                     /translation="MAYDVEAWSDFFVATAGATAALAGLLFVAVSINLERILALPGVA
                     DRALGTLVLLLGALLVAIFALAPGQSDLAVGLEVAGTGGLTTAVAGRLAMRIQADQRS
                     HMISVAVISFWGTVPFLVGGLSLAEGTGGGLYWVLAGTVGAIAGAVSNAWVVLVEILR
                     "
     gene            complement(1786..>3314)
                     /locus_tag="VFY44_04410"
     CDS             complement(1786..>3314)
                     /locus_tag="VFY44_04410"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEX5909712.1"
                     /translation="GALMQQVSERGASIETALLFFDLEWNELEDAKAEKLLEADELSK
                     VRHYLRTLRRYRDHQLTEPEERILTETQVTGGSAFARLFTELTSSVEVDLPGSEDPVP
                     LMEALSLLQSPDRDQRRTAAEGVTAALQPGLRTRAYIFNTLLQDKSTKDRLRSYPHWL
                     ASRNLANEASDESVTALIDAVVGRYDLARRWYQLKARLLGLDKLEYYDRMAPVAEAEQ
                     HIPYDEAKATVLDCYRNFSAELGDVASSFFEGSYIDAPPLPGKQGGAFCAYTVPSAHP
                     YVLLNYTSRPGDVLTMAHELGHGVHAALARPKGMFEFTTPLTVAETASIFGETIVLER
                     LLTDAPDASARLSLLAESLDGAVAAVFRQIAMNRFEHRIHTERRESGELSTDAFAAAW
                     SDTQADLLGDSVAPSEDYASWWSYVPHFVNTPGYVYAYAYGHLLALSVYRRFEEEGPG
                     FTPAYLDLLRAGGSMPPEELGKIVGVDLTDAGFWSSGLDLIERQLDLAEKAAEEAGRG
                     "
BASE COUNT          501 a         1174 c         1195 g          444 t
ORIGIN      
        1 ccgggcatgg ccgcgcccgc gccggagccg aacccggtga ccgtgcacga gatcagccgc
       61 gggttggccg ccgacagggt ctcgtggtcc aggcccaggc gctccatgac gccgggccgg
      121 aagttctgga caaccacgtc cgccccctcg gccagctcgc gcgcgaccgc caggtcggcg
      181 tcgtcggcca ggtccagcgc cacgctgtgc ttgccgcggt tcaccgacag gaagtaggtg
      241 gccgtgccgt cgggtgcgtg gggcgggccc cagccgcggg tgtcgtcacc ccggcccggg
      301 cgctcgacct tgagcacggt cgcgccgtgg tcggccagga gcatggtggc cagcggggcg
      361 gccaggacgc gggagaagtc caggaccacc aggtcgccga gcggagcatt gcccatgcgc
      421 ccagatggtt tcagacgccg cgggacgagc atgcgacggg ggatgcctac cgacacgaca
      481 gtcccagccg tcacgctcaa cgacggggtc gcgatccccc agctggggtt cggcgtcttc
      541 cagatcccgc ccggcgacac cgtcgaggcc accctgcgcg cgttccagac cggctaccgg
      601 cacatcgaca ccgcggccgc ctaccagaac gaggcggagg tcggccaggc cttccgcgcg
      661 tcggggctgg accgcgacga ggtgttcatc accaccaagt gcttcaacga ctcgcacggc
      721 tacgagcagg ccaagcaggc gttccagtcc agcatcgacc gcctggagct ggagttcgtg
      781 gacctgtacc tgatccactg gccggtgccg tcccaggaca agtacgtgga gacctggaag
      841 gcgttcatcg agctcaagga gcagggcctg atccgctcca tcggcgtgtc caacttccag
      901 ccgccgcacc tgcgccggat catcgacgag acgggcgtga cgccgtcggt caaccaggtc
      961 gagctgcacc cgcgcttcca gcaggggggc ctgcggcgcg agcacgagga cctgggcgtc
     1021 gtgaccgagg cctggagccc gctggcccag ggcgccgtgc tggacgaccc cgtgctgggg
     1081 gcgatcgccg aggagcacgg caagaccacg ggccaggtgg tgctgcgctg gcacgtccag
     1141 ctgggcaacg tggtgttccc caagtccgtc acccccgagc ggatcgagga gaacttcggg
     1201 atcttcgact tccacctgtc cgaggacgag atggggcgga tcgaggcgct ggacgcgggc
     1261 gagcgccagg gcccggaccc ggacacgttc gtccggccct agcgcaggat ctcgaccagc
     1321 acgacccagg cgttggacac cgcgccggcg atggctccca cggtcccggc caggacccag
     1381 tagagcccgc cgccggtgcc ctcggccagg ctcaggccac cgaccaggaa cggcacggtg
     1441 ccccagaagc tgatgaccgc gacgctgatc atgtgcgacc gctggtcggc ctggatgcgc
     1501 atggccagcc ggcccgccac ggccgtcgtg agcccgccgg tgccggcgac ctcgagcccg
     1561 accgccaggt cggactgtcc cggcgcgagc gcgaagatcg ccacgagcag cgcgcccagg
     1621 agcagcacca gcgtgcccag cgcccggtcg gccacgccgg gcagggcgag gatccgttcg
     1681 aggttgatcg acaccgccac gaacagcagg ccggccagcg ccgcggtggc gccggcggtg
     1741 gctacgaaga agtcgctcca ggcctcgacg tcgtaggcca tcgcctcagc cgcgcccggc
     1801 ttcctccgcc gccttctcgg ccaggtccag ctggcgctcg atcaggtcca gccccgagct
     1861 ccagaagccg gcgtcggtca ggtccacgcc gacgatcttg cccagctcct cgggcggcat
     1921 cgatccgccg gcgcgcagca ggtcgaggta ggcgggggtg aagcccgggc cctcctcctc
     1981 gaaccggcgg tacaccgaca gcgccagcag gtggccgtag gcgtaggcgt acacgtagcc
     2041 cggcgtgttc acgaagtggg ggacgtacga ccaccacgag gcgtagtcct cggacggggc
     2101 gaccgagtcg ccgagcaggt cggcctgggt gtccgaccag gcggcggcga acgcgtcggt
     2161 ggacagctcg ccggactcgc gccgctcggt gtggatgcgg tgctcgaacc ggttcatggc
     2221 gatctggcgg aagaccgcgg ccacggcgcc gtccagcgac tcggccagca gcgacagccg
     2281 cgccgacgcg tcgggggcgt cggtcagcag ccgctccagc acgatcgtct cgccgaagat
     2341 cgaggcggtc tcggccacgg tcagcggcgt cgtgaactcg aacatgccct tggggcgggc
     2401 cagcgcggcg tgcacgccgt ggcccagctc gtgcgccatg gtcagcacgt cgccgggccg
     2461 cgaggtgtag ttcaggagca cgtacgggtg cgccgacgga acggtgtacg cgcagaacgc
     2521 gccgccctgc ttgcccggca gcggcggggc gtcgatgtac gagccctcga agaaggacga
     2581 cgcgacgtcg cccagctcgg ccgagaagtt gcggtagcag tccagcaccg tcgccttggc
     2641 ctcgtcgtag gggatgtgct gctcggcctc ggccaccggc gccatccggt cgtagtactc
     2701 gagcttgtcc agccccagca gccgggcctt gagctggtac cagcgccggg ccaggtcgta
     2761 gcggccgacc acggcgtcga tcagcgcggt cacggactcg tcgctggcct cgttggccag
     2821 gttccgcgac gccagccagt gcgggtagga gcgcaggcgg tccttggtcg acttgtcctg
     2881 cagcagcgtg ttgaagatgt acgcgcgggt gcgcagcccc ggctgcagcg cggcggtcac
     2941 gccctcggcg gccgtgcggc gctggtcacg gtcggggctc tgcaggaggc tgagcgcctc
     3001 catcagcggc actgggtcct cgctgccggg caggtccacc tcgaccgacg acgtcagctc
     3061 ggtgaacagc cgcgcgaagg ccgagccgcc ggtcacctgg gtctcggtga ggatgcgctc
     3121 ctcgggctcg gtcagctggt gatcgcggta gcggcgcagc gtgcgcaggt agtgacgcac
     3181 cttggacagc tcatcggcct ccagcagctt ctcggccttc gcgtcctcca gctcgttcca
     3241 ctcgaggtcg aagaacagca gcgcggtctc gatcgaggct ccgcgctcgg acacctgctg
     3301 catcagggcg ccgg
//
LOCUS       DASUPW010000191         2217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1293694, whole genome shotgun sequence.
ACCESSION   DASUPW010000191 DASUPW010000000
VERSION     DASUPW010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293694"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..1480
                     /locus_tag="VFY44_04415"
     CDS             248..1480
                     /locus_tag="VFY44_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5909713.1"
                     /translation="MSRSRTIFLVATGLVALHVADDSFLQPQAGTTAGDHLVSGLVPL
                     AGLGAAVWMTARTGDGARAAIDLLLGVFGIVAGLEAVHYAREVGPTGDDFTGLLSIPA
                     GIALIGLGLVTVWTTRDREGSLGRRVLRRTGIGLAAFVVFQFVVFPLSVGYVVTHAAR
                     PPVEHIDLGGRDTDVRFRTNDGLRLHGSYVPSRNGAAVIVAFGRKGSQPHARMLARHG
                     YGVLIFDRRGEGHSDGDPNSYAWSDGERDLRAAVDFLQRRPDVDPGRIGGLGLSVGGE
                     TFLQAAAHDRDIRAVVSEGASARSFGELRSVPGSAPLTAATMAGITAGTAVFSNATPP
                     PHLKDLVGRIAPRPMLLIYATEGTGGEEHTFNRAYHRAARAPKAIWEIPESGHVGGLE
                     ARPREYERRVAGFFDRSL"
     gene            complement(1477..1992)
                     /locus_tag="VFY44_04420"
     CDS             complement(1477..1992)
                     /locus_tag="VFY44_04420"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5909714.1"
                     /translation="MSDRPPFDPIAEAARQWEAHWGPEPVAAMTGVTSIMRVQQILMA
                     RLNALLKPWELSFPRYEALMLLFYSRRGSLPMGKMGARLQVHPTSVTSLIDGLEGMGL
                     ARREPHEHDRRTTLASITPEGRRVAEQATAALHGARFGLDGLDDDQLAEVSGLLDRLR
                     EGAGDIAPARA"
     gene            2037..>2217
                     /locus_tag="VFY44_04425"
     CDS             2037..>2217
                     /locus_tag="VFY44_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX5909715.1"
                     /translation="MSQAYQQTPLEGWEEAYAATPERDAEFSTMSGIPIDPLYAPEEV
                     DEKIGYPGQYPYTRGV"
BASE COUNT          290 a          824 c          796 g          307 t
ORIGIN      
        1 gctgatccgg ggcgtgctgc tcccggcgac catgaagctc ctgggcgacc gcaactggtg
       61 gctgcccagg aagctgaact ggctgcccca ggtcgagcac gagggtgagg tggagccggt
      121 cacggcgtag acccctcaca taccgaagca ccggtgcccg ccctcaggcg ggcaccggtc
      181 gtttcagggg gtcggatcag ggtctccgcc cgatgtccgg atcccgctcc cggagcagca
      241 tggggccatg agccgatcac gcaccatctt cctcgtggcc accggtctcg tggccctgca
      301 cgtggccgac gactcgttcc tccagcccca ggccggcacc accgccggcg accatctggt
      361 cagcggcctg gtgccgctgg cggggctcgg tgccgcggtc tggatgaccg cccggaccgg
      421 cgacggcgcc cgcgccgcga tcgacctgct gctgggggtc ttcgggatcg tggcggggct
      481 cgaggccgtg cactacgcca gggaggtcgg ccccacgggc gacgacttca ccgggctgct
      541 gtccataccc gccgggatcg cgctgatcgg gctcggcctg gtcaccgtct ggaccacccg
      601 tgaccgcgag ggcagcctcg ggcgccgcgt gctccggcgg acgggcatcg ggctcgccgc
      661 gttcgtggtg ttccagttcg tcgtcttccc gctctcggtg gggtacgtgg tcacgcatgc
      721 ggcgcgtccg ccggtcgagc acatcgacct cggcggccgt gacaccgacg tccgcttccg
      781 gaccaacgac ggcctcaggc tgcacggctc gtacgtgcct tcgcgcaacg gggccgccgt
      841 gatcgtggcc ttcggccgca agggcagcca gccgcacgcc cggatgctgg cccgccacgg
      901 ctacggggtg ctgatcttcg accgccgggg cgagggccac agcgacggcg accccaactc
      961 ctacgcgtgg tccgacggcg agcgggacct gcgcgccgcg gtcgacttcc tccagcgccg
     1021 gccggacgtc gaccccggcc ggatcggcgg cctcggcctg tccgtcggcg gggagacctt
     1081 cctccaggcc gccgcgcacg accgcgacat ccgcgcggtg gtcagcgagg gggcgtccgc
     1141 gcgctcgttc ggcgagctgc gcagcgtgcc cgggtcggcg ccgctgaccg ccgccaccat
     1201 ggcgggcatc accgccggca ccgcggtgtt ctcgaacgcc acgccgccgc cgcacctgaa
     1261 ggacctggtc ggccggatcg cgccgaggcc gatgctgctg atctacgcca ccgagggcac
     1321 ggggggcgag gagcacacgt tcaaccgcgc ctaccaccgc gccgcgcgcg ccccgaaggc
     1381 catctgggag atccccgagt ccgggcacgt cggcggcctc gaggcgcgcc cacgcgagta
     1441 cgagcgccgg gtcgccgggt tcttcgaccg gtcgctctag gcccgggccg gcgcgatgtc
     1501 gccggcgccc tcgcgcaggc ggtccagcag ccccgacacc tcggccagct ggtcgtcgtc
     1561 caggccgtcg aggccgaagc gcgccccgtg cagcgccgcg gtcgcctgct cggccacgcg
     1621 ccgcccctcg ggggtgatcg aggccagcgt ggtgcgccgg tcgtgctcgt gtggctcgcg
     1681 ccgggccagg cccatgcctt cgagcccgtc gatcaggctg gtcacgctgg tggggtggac
     1741 ctgcagccgg gcgcccatct tgcccatggg cagggagccg cggcggctgt agaacagcag
     1801 catcagcgcc tcgtagcgcg ggaacgacag ctcccacggc ttgagcagcg cgttcagccg
     1861 cgccatcagg atctgctgca cgcgcatgat cgacgtgacg cccgtcatgg ccgccacggg
     1921 ctcggggccc cagtgggcct cccactgacg ggcggcttcg gcgatggggt cgaagggggg
     1981 ccggtcgctc atgcgcattg cagatactag gacatccgac tatcgtcctc ccaaccatga
     2041 gccaggccta ccagcagacg cccctcgaag gctgggagga ggcgtacgcc gccacccccg
     2101 agcgcgacgc cgagttctcg acgatgtcgg ggatcccgat cgatccgctg tacgcgcccg
     2161 aggaggtcga cgagaagatc ggctaccccg gccagtatcc gtacacgcgc ggggtgt
//
LOCUS       DASUPW010000192         5148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1570458, whole genome shotgun sequence.
ACCESSION   DASUPW010000192 DASUPW010000000
VERSION     DASUPW010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5148
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570458"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VFY44_04430"
     CDS             <1..516
                     /locus_tag="VFY44_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909716.1"
                     /translation="DPPAFDILFWNADSTNLPAALHRDFLELSLENSITHPGELTVLD
                     TPVDLTKVKAPTYVVAGIADHIIPWQNAYKSVHLYGSDPRFVLSTSGHIAALVNPPGN
                     EKASFQTNPANPESPDEWLEGASKKRGSWWEDWSEWLAEHSGDDKRKPAKLGQRGFTP
                     LGDAPGEYVHG"
     gene            complement(473..1333)
                     /locus_tag="VFY44_04435"
     CDS             complement(473..1333)
                     /locus_tag="VFY44_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909717.1"
                     /translation="MTSGAQPLDVLADGVEGASLDPAPPGSQGEPRRWWGNHLGEFRW
                     QAELARLMVDPVYRGQGIPRGDGAPVVLIPGLFAGDGSLEVMARWLKRMGYDARGSGI
                     VSNVDCSDRALDRLEHRVARVRGETGRKVALIGHSRGGHFAKALSHRHPDWISASISM
                     GAGLDQPFDVSVPLKGVLKVARGVFARTSDRVERNGCFTEHCRCKFTADFTGTVPEEV
                     PFTAIYSKGDGVVWWEACRADWATNIEVTGSHVGLAFNRKVYKAVAETLAEHTALSRE
                     RTPRERPLAA"
     gene            1558..3252
                     /locus_tag="VFY44_04440"
     CDS             1558..3252
                     /locus_tag="VFY44_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5909718.1"
                     /translation="MALIEDSPEYALLVREMLGAATDGPLEIAHFEDMNAALPHLRDQ
                     QVDCVLMDLGLPDTTGLGGLAQVQSVAPDLPIVVLSGEQSEEIAVQAVHEGAQDYLVK
                     RSATGEHVLRSVRYAMERKRAQVALAHQALHDSLTGLPNRTLLMDRTDQALARAERTQ
                     GRVALLFLDLDRFKLVNDSLGHDAGDRLLCRVAERLKSLIRPSDTVARFGGDEFMVLC
                     DRVEDDRQAILVAERLSGGLAEPFVLEGRELFVGASIGIAFGADRSATAVSLIRDADQ
                     AMYRAKERGSRYEMADGTASRRAAVRLSTETQLHRALDRKELRLFFQPEVDLIRNSIF
                     SVEALLRWQHPDRGMLGPGDFLSSAEENGLIVPIGEWVIDTAATQLAGWRNAGLCAPD
                     MSTSINLSLRQLAEPGLLETVQGAIERAGIPPEALCFEITESVVAADEAEVMRHLGQL
                     RELGVGLSLDDFGVGLSSLSALDRYPLDMLKIDRSFVGRLASGGERAERMFSAVLGAA
                     RAVGLKAVAEGIETRSQLETVKRLGCDAAQGFYLCRPEAAGTLSPRLLSLTPSAVA"
     gene            3252..>5148
                     /locus_tag="VFY44_04445"
     CDS             3252..>5148
                     /locus_tag="VFY44_04445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5909719.1"
                     /translation="MSAVAAAPATGRTRRIGRWRALSTVQSVQVALLTVAGVTGVAGV
                     LTYVLHEDLLAPYPGVTPFAFNVGLAICALTVGVILDGHRASRALGAFVAVVGALTLV
                     EHASGTDLGIGRVLFDPFRETSVGRMAPNTAVCVTFAGVALATRGRVSTVLAAVPALI
                     GLMAALGYTDPDAKALASIGQSVNMSLPTAVGLCTSSLALILARERGFFTRPTGGGTI
                     IRRLGLMVLLLPAVGGSVLVAGIRNDVFSAASGIWLLTVAAVLSALGALFWVASVVDR
                     EEENTRATLALQTETAANLTEGLCLRRESDGVIISVNPALKEIFGYGAGDLTGTSRRL
                     LTEENERRMRQELADHGAWMTEVETERADGSLFWCSVKASNFEDPEHGLMRVALYHDV
                     TERREAEARRRQAEKRSAAALVELERSNAELEQFAHVASHDLSEPLRVVGGFVGLLQK
                     RYEGKLDDDADRFIDATVSGVERMQALIDALLAYSRVGSGDLQVDAVDTGRVAAEAVR
                     VQERRIEEAGGEVVIGDLPTVPGDAVLLERVFQNLVSNAMKFRADGVAPRVTIGAERS
                     DFGVWHFEVEDNGMGIPPEHAERIFGMFQRLQGRQMEGTGIGLSITRRILERHCGRIW
                     AEACDGGG"
BASE COUNT          713 a         1857 c         1878 g          700 t
ORIGIN      
        1 gacccgcccg cgttcgacat cctgttctgg aacgccgact cgaccaacct gccggccgcg
       61 ctgcaccgcg acttcctgga gctgtcgctg gagaactcga tcacccatcc gggcgagctg
      121 accgtgctgg acaccccggt cgacctgacc aaggtcaagg cgcccaccta cgtcgtcgcg
      181 gggatcgccg accacatcat cccgtggcag aacgcctaca agtccgtgca cctgtacggc
      241 tcggacccca ggttcgtgct gtccaccagc ggccacatcg cggcgctggt gaacccgccc
      301 ggcaacgaga aggcctcgtt ccagaccaac cccgccaacc cggagtcgcc cgacgagtgg
      361 ctggagggcg cctcgaagaa gcgcggctcg tggtgggagg actggtcgga gtggctggcc
      421 gagcactccg gggacgacaa gcgcaagccg gccaagctcg gccagcgggg cttcacgccg
      481 ctaggggacg ctcccgggga gtacgttcac ggctgagcgc cgtgtgctcg gccagcgtct
      541 cggccaccgc cttgtagacc ttgcggttga aggccagccc cacgtggctg ccggtgacct
      601 cgatgttggt ggcccagtcg gcgcggcacg cctcccacca gaccacgccg tcgcccttcg
      661 agtagatggc ggtgaacggc acctcctcgg gcaccgtgcc ggtgaagtcg gcggtgaact
      721 tgcaccggca gtgctcggtg aagcagccgt tgcgctcgac ccggtcgctg gtgcgcgcga
      781 acacaccgcg ggccaccttg agcacgccct tgagcggcac gctgacgtcg aacggctggt
      841 ccaggccggc gcccatcgag atggacgccg agatccagtc gggatggcgg tggctcagcg
      901 ccttggcgaa gtgcccgccg cggctgtgcc cgatcagcgc gaccttgcgc ccggtctccc
      961 cgcgcacgcg cgcgacccgg tgctccaggc ggtccagggc gcggtccgag cagtcgacgt
     1021 tgctgacgat gcccgagccg cgggcgtcgt agcccatgcg cttcagccag cgggccatga
     1081 cctccagcga gccgtcgccg gcgaacaggc cggggatgag caccaccggg gcgccgtcgc
     1141 cgcgcgggat gccctgcccc cggtacacgg gatcgaccat cagccgggcc agctcggcct
     1201 gccaccggaa ctcgcccagg tggttgcccc accagcggcg gggctcaccc tggcttccgg
     1261 gcggcgcggg gtccagggag gccccctcca cgccgtcggc caacacgtcc agcggctgcg
     1321 cccccgaggt catggcgaaa ggctagcgcc gggcccgggt cgctcccgcg ccaaccgtgc
     1381 ttgagcgcgg acgcgccaac tggacggatg ttcgatccag gggctgtccg aaggggtcga
     1441 gaggctgagc agcagcacgc ccgccccggc caaggactcg cctccgtgcc gactgaaccc
     1501 gccccaagcg cccgccccag ccacccccca cccgagcccg gcctgtccac gctccacgtg
     1561 gccctgatcg aggacagccc cgagtacgcg ctgctggtgc gcgagatgct cggcgcggcc
     1621 accgacggcc cgctcgagat cgcccacttc gaggacatga acgccgcgct gccccacctg
     1681 cgcgaccagc aggtggactg cgtgctgatg gacctggggc tgcccgacac caccggcctg
     1741 ggcgggctgg cgcaggtgca gtcggtggca ccggacctgc cgatcgtggt gctcagcggg
     1801 gagcagagcg aggagatcgc cgtccaggcc gtgcacgagg gcgcgcagga ctacctggtc
     1861 aagcgctcgg cgacgggtga gcacgtgctg cgctccgtgc gctacgccat ggagcgcaag
     1921 cgcgcccagg tggcgctggc ccaccaggcg ctgcacgact cgctgaccgg cctgcccaac
     1981 cggaccctgc tgatggaccg caccgaccag gcgctggccc gggccgagcg gacccagggc
     2041 cgggttgccc tcctgttcct ggacctggac cggttcaagc tggtcaacga cagcctgggc
     2101 cacgacgccg gcgaccggct gctgtgccgg gtggccgagc ggctcaagag cctgatccgc
     2161 ccgtccgaca ccgtcgcccg cttcggcggc gacgagttca tggtgctgtg cgaccgggtc
     2221 gaggacgacc gccaggcgat cctggtggcc gagcgcctca gcggcgggct ggccgagccc
     2281 ttcgtgctcg agggccgcga gctgttcgtg ggcgcctcga tcgggatcgc cttcggcgcc
     2341 gaccgcagcg ccaccgcggt gagcctgatc cgcgacgccg accaggccat gtaccgcgcg
     2401 aaggaacgcg ggagccggta cgagatggcc gacggcaccg cctcccggcg cgccgcggtg
     2461 cggctgagca ccgagacgca gctgcaccgg gcgctggacc gcaaggagct ccggctcttc
     2521 ttccagcccg aggtggacct gatccgcaac tccatcttca gcgtcgaggc gctgctgcgc
     2581 tggcagcacc ccgaccgggg catgctgggc cccggcgact tcctgagcag cgccgaggag
     2641 aacgggctga tcgtgcccat cggcgagtgg gtcatcgaca ccgcggccac ccagctggcc
     2701 ggctggcgca acgccggcct gtgcgcgccc gacatgagca ccagcatcaa cctctcgctg
     2761 cgccagctgg ccgagccggg cctgctggag accgtgcagg gggccatcga gcgcgcgggg
     2821 atcccgcccg aggcgctgtg cttcgagatc accgagagcg tcgtggccgc cgacgaggcc
     2881 gaggtcatgc ggcacctggg ccagctgcgc gagctgggcg tcgggctgag cctggacgac
     2941 ttcggcgtcg ggctgtcctc gctgagcgcg ctggaccgct atccgctgga catgctcaag
     3001 atcgaccgtt cgttcgtcgg ccggctggcc agcggcgggg agcgcgccga gcgcatgttc
     3061 tcggccgtgc tgggcgccgc ccgtgccgtg gggctcaagg ccgtggccga gggcatcgag
     3121 acgcggtcgc agctggagac cgtcaagcgc ctgggctgcg acgcggccca gggcttctac
     3181 ctctgccggc ccgaggcggc cggcacgctg agcccgcgcc tgctgagcct gaccccgagc
     3241 gcggtggcct gatgagcgcg gtcgctgccg ccccggcgac cggccggacc cgccgcatcg
     3301 gccgctggcg cgcgctgtcc accgtccagt cggtccaggt ggcgctgctc accgtggccg
     3361 gcgtcaccgg tgtggccggg gtgctgacgt acgtgctcca cgaggacctg ctggcgccct
     3421 accccggggt gacgccgttc gcgttcaacg tcgggctggc catctgcgcg ctgaccgtcg
     3481 gcgtgatcct ggacggccac cgggcgtcgc gtgcgctcgg cgcgttcgtg gccgtggtgg
     3541 gcgccctgac cctggtcgag cacgccagcg gcaccgacct gggcatcggg cgggtgctgt
     3601 tcgacccctt ccgcgagacc tccgtgggac gcatggcgcc caacaccgcg gtctgcgtga
     3661 ccttcgccgg cgtcgcgctg gccacccgcg gccgtgtgtc gaccgtcctg gccgcggtcc
     3721 ccgcgctgat cggcctgatg gccgcgctgg gctacaccga ccccgacgcc aaggcgctgg
     3781 cgtcgatcgg ccagagcgtg aacatgtccc tgcccacggc cgtgggcctg tgcaccagca
     3841 gcctggcgct gatcctggcg cgggagcgcg gcttcttcac ccgccccacc ggtggcggca
     3901 ccatcatccg ccgcctcggg ctgatggtgc tgctgctgcc ggccgtcggc ggctcggtcc
     3961 tggtcgccgg gatccgcaac gacgtgttct cggcggcgtc gggcatctgg ctgctgaccg
     4021 tggccgccgt gctgtccgcg ctcggggcgc tgttctgggt cgcgagcgtg gtcgaccgcg
     4081 aggaggagaa cacgcgcgcc acgctggcgc tgcagaccga gaccgcggcg aacctgaccg
     4141 agggcctgtg cctgcgacgc gagagcgacg gggtgatcat ctcggtgaac cccgcgctga
     4201 aggagatctt cggctacggc gccggcgatc tgaccgggac cagccgccgg ctgctgaccg
     4261 aggagaacga gcgccgaatg cgccaggagc tggccgacca cggcgcctgg atgaccgagg
     4321 tcgagaccga gcgcgccgac ggctcgctgt tctggtgctc ggtcaaggcg tcgaacttcg
     4381 aggaccccga gcacggcctg atgcgcgtcg cgctctacca cgacgtgacc gagcgccgcg
     4441 aggccgaggc gcgccggcgc caggccgaga agcgcagcgc cgccgcgctg gtggagctgg
     4501 agcgctcgaa cgccgagctg gagcagttcg cgcacgtggc ctcccacgac ctgagcgagc
     4561 cgctgcgcgt ggtcggcggg ttcgtcgggc tgctccagaa gcgctatgag ggcaagctcg
     4621 acgacgatgc cgaccgcttc atcgacgcca cggtgtcggg cgtcgagcgg atgcaggcgc
     4681 tgatcgacgc gctcctggcc tactcccgcg tgggctcggg cgacctccag gtggacgcgg
     4741 tggacaccgg gcgcgtggcg gccgaggccg tccgggtgca ggagcggcgg atcgaggagg
     4801 cggggggcga ggtggtcatc ggcgacctgc ccaccgtgcc cggggacgcg gtgctgctgg
     4861 agcgcgtgtt ccagaacctg gtgtccaacg ccatgaagtt cagggctgac ggggtggccc
     4921 cgcgcgtgac catcggcgcc gagcgctcgg acttcggggt ctggcacttc gaggtcgagg
     4981 acaacgggat gggcatcccg cccgagcacg ccgagcgcat cttcggcatg ttccagcggc
     5041 tccagggacg ccagatggag ggcacgggga tcgggctgtc catcaccagg cgcatactcg
     5101 agcgacactg tggtcgtatc tgggccgaag cgtgcgacgg cgggggga
//
LOCUS       DASUPW010000193         2787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1017375, whole genome shotgun sequence.
ACCESSION   DASUPW010000193 DASUPW010000000
VERSION     DASUPW010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017375"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="VFY44_04450"
     CDS             complement(<1..1496)
                     /locus_tag="VFY44_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HEX5909720.1"
                     /translation="MAKDAKNGNGNGSYGAQDITVLEGLEAVRKRPGMYIGSTGIRGL
                     HHLIYEVMDNSVDEALAGEADAVSITIHPDNAVTVTDNGRGIPVGIMEKEGKPAAEVV
                     LTVLHAGGKFGDGGGYKVSGGLHGVGISVVNALTEKLDLTIWTEGHEHTQSYERGIPQ
                     GELTKGAKTDRTGTSITFMPDAEIFETVEYERETLEQRFREMAFLTRGLKIDFADERA
                     EGFKATFKYDGGIVDFVKHLHSQGTRDPLHKKIVFITEETEVGEVEVALQWNTSYQEN
                     LLSFANNINTIEGGTHLSGFRSALTRTINAYARSKGELKEKDNNLSGEDVREGLTAIV
                     SVKVQDPQFEGQTKTKLGNPPVEGFVQAAVNRGLAEFLEENPADGRDIIRKAVSASRA
                     REAARKARDLTRRKSALENTPLPGKLADCSVRDPALAEIFIVEGDSAGGSAKQGRDRN
                     TQAVLPLRGKIINVEKNRIDKVLSNNEIQAMITAIGTGIRDEFDIERAR"
     gene            complement(1714..2031)
                     /locus_tag="VFY44_04455"
     CDS             complement(1714..2031)
                     /locus_tag="VFY44_04455"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEX5909721.1"
                     /translation="MRRREPRPLGAALEGVVGAARPQTLLARVQGVWEEAVGATVAAE
                     AQPRAESSGTVAVECSSGVWAQELELLAPDLIDRLNDALGAPDHGPVRALRLRVGRPL
                     RGV"
     gene            complement(2028..>2787)
                     /gene="recF"
                     /locus_tag="VFY44_04460"
     CDS             complement(2028..>2787)
                     /gene="recF"
                     /locus_tag="VFY44_04460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HEX5909722.1"
                     /translation="FLPERLELVKGAPSPRRAHLDRFVAAVWPARAEARTAYSRALSQ
                     RNALLGRIRAGASSPATLDTWDAELARHGLQLMGDRREAVTLLAPRFAELGTLLGLPD
                     AVALEYRPRSPAADPAELAAELAERRTADLERGFTAHGPHRDDLRLVHGGRSLRQYGS
                     QGQQRVALLALLFAERDVLRDQRGTPPLMLLDDVMSELDGSRRALLADLLREGGQSLL
                     TTTELEHVPDAGDEGTTVIEVERGSLHPAVGATP"
BASE COUNT          341 a          981 c         1007 g          458 t
ORIGIN      
        1 cgggcccgct cgatgtcgaa ctcgtcgcgg atgcccgtgc cgatggccgt gatcatggcc
       61 tggatctcgt tgttcgagag gaccttgtcg atccggttct tctcgacgtt gatgatcttg
      121 ccgcgcaggg gcagcaccgc ctgcgtgttg cggtcgcggc cctgcttggc cgagccgccg
      181 gccgagtcgc cctcgacgat gaagatctcg gccagcgccg gatcgcgcac cgagcagtcg
      241 gcgagcttgc cggggagcgg ggtgttctcc agcgccgact tgcgccgggt caggtcgcgc
      301 gccttgcgcg cggcctcgcg tgcgcgggat gccgagacgg ccttgcggat gatgtcgcgg
      361 ccgtccgcgg ggttctcctc gaggaactcg gccaggccgc ggttgaccgc ggcctgcacg
      421 aagccctcga ccggcgggtt gccgagcttg gtcttggtct ggccctcgaa ctgcgggtcc
      481 tggaccttca ccgacacgat ggccgtcagg ccctcgcgca cgtcctcgcc gctgaggttg
      541 ttgtccttct ccttgagctc gcccttcgag cgcgcgtacg cgttgatggt ccgcgtcagc
      601 gccgaccgga agccggacag gtgcgtgccg ccctcgatcg tgttgatgtt gttggcgaac
      661 gagagcaggt tctcctggta ggaggtgttc cactggagcg ccacctccac ctcgccgacc
      721 tcggtctctt cggtgatgaa cacgatcttc ttgtgcaggg gatcgcgtgt gccctggctg
      781 tgcaggtgct tgacgaagtc gacgatgccg ccgtcgtact tgaaggtcgc cttgaagccc
      841 tcggcgcgct cgtccgcgaa gtcgatcttc aggccccgcg tcaggaaggc catctcgcgg
      901 aagcgctgct ccagcgtctc gcgctcgtac tcgacggtct cgaagatctc ggcgtcgggc
      961 atgaacgtga tgctcgtgcc ggtgcggtcg gtcttcgcgc ccttggtcag ctccccctgg
     1021 gggatgccgc gctcatagct ctgggtgtgc tcgtgcccct cggtccagat cgtgaggtcg
     1081 agcttctcgg tcagcgcgtt gaccacggag atgccgacgc cgtgcaggcc accggagacc
     1141 ttgtagcccc cgccgtcgcc gaacttgccg cccgcgtgca ggacggtgag gacgacctcg
     1201 gcggccggct tgccctcctt ctccatgatc cccacgggga tgccgcgccc gttgtcggtc
     1261 acggtgaccg cgttgtcggg gtggatcgtg atcgacaccg cgtcggcctc gccggccagc
     1321 gcctcgtcga cggagttgtc catcacctcg tagatcaggt ggtgcagccc gcggatcccg
     1381 gtcgatccga tgtacatgcc ggggcgcttg cgcacggcct ccaggccctc gaggacggtg
     1441 atgtcctgcg cgccgtagct gccgttcccg ttgccgttct tcgcgtcctt cgccaagcga
     1501 aacctttcgt caccacatac gtgaagaggc cccggtcggg cggatgatcg ccacgctccg
     1561 gggcactcgg tgagccggtc ttagatatta gcaggatagg tcccgcaccg gacgttcccg
     1621 ggcgttacaa aaggcctgca aatccgcaca aaacgcgtga cccggccgcg ggctggttgt
     1681 cggtcgttat ggcgatggtc acgcgggggc gtctcacacc cctctgaggg ggcggcccac
     1741 gcgcagcctc agggcccgga ccgggccgtg gtcgggggcg ccgagggcgt cgttgaggcg
     1801 gtcgatcagg tcgggggcca gcagctcgag ctcctgcgcc cacacccccg acgagcactc
     1861 caccgccacc gtgccggacg actccgcccg cggctgggcc tcggcggcca cggtcgcgcc
     1921 gacggcctcc tcccagacgc cctgcacccg ggccagcagc gtctgcgggc gggccgcccc
     1981 gacgacgccc tcgagggccg ccccgagcgg tcgcggctcg cgccgtctca cggggtggcc
     2041 cccaccgcgg gatggagcga cccgcgctcg acctcgatca ccgtcgtccc ctcgtcgccg
     2101 gcgtcgggca cgtgctccag ctcggtcgtg gtgaggaggc tctggccgcc ctcgcgcagg
     2161 aggtcggcca ggagggcgcg gcgcgagccg tcgagctcgg acatgacgtc gtcgaggagc
     2221 atcagcggcg gcgtcccgcg ctggtctcgc aggacgtcgc gctcggcgaa caggagggcc
     2281 aggagggcga cccgttgctg gccctgggat ccgtactggc gcagcgagcg cccgccgtgg
     2341 accaggcgca ggtcgtcgcg gtgtggcccg tgggcggtga acccgcgctc gaggtccgcg
     2401 gtccggcgct ccgccagctc cgcggccagc tcggccgggt cggcggcggg cgagcgcggc
     2461 cggtactcca gcgcgacggc gtcgggcaag ccgaggaggg ttccgagctc ggcgaagcgg
     2521 ggcgccagca gcgtcaccgc ctcgcgccgg tcgcccatca gctggaggcc gtgccgcgcg
     2581 agctcggcgt cccaggtgtc cagcgtcgcc gggctgctcg cccccgcccg gatgcgcccc
     2641 aggagcgcgt tgcgctggct cagggcgcgc gagtaggcgg tgcgggcctc ggcgcgggcg
     2701 ggccacacgg ccgccacgaa ccggtccagg tgggcacggc gcggtgacgg tgcgcccttg
     2761 acgagctcga ggcgctcggg caggaac
//
LOCUS       DASUPW010000194         4832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_186970, whole genome shotgun sequence.
ACCESSION   DASUPW010000194 DASUPW010000000
VERSION     DASUPW010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186970"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="VFY44_04465"
     CDS             complement(<1..1014)
                     /locus_tag="VFY44_04465"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909723.1"
                     /translation="MVVCVLIPRFALLAALGDRRALLSEPVALAPEAGREQTIGEVSA
                     PAEAFGIVAGMRVGEALSRCPELRLEPPDPEGVRSLWHEVLDRIESIGAAPESQHAGA
                     AFFAAGGLEGLHGGDLAGVLDATRRALGPGSRLGAAESRFAAHCAARQTRPRRAPVVV
                     GQDETTRFLAPLPAALLRSRPELAELPDVLERLGIRTLGDLAALPSRAVAERFGHPGL
                     LALDLARGRDTPLVPRRPPEPVSERIDLPDAASGPQLERALELLIARALARRERRGRT
                     MRSLAVAARFVAGGTWRCAVTLRHASADPGRIALALTPKLAELPAPAESLALEVEAFG
                     PPAT"
     gene            1317..2213
                     /locus_tag="VFY44_04470"
     CDS             1317..2213
                     /locus_tag="VFY44_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909724.1"
                     /translation="MARDTGMPETDASFDFARERRRRAVGKLSSTLRLAPADVNVILP
                     FDEVVEALGRRGEKRLGLKSIALDSIVGTVDRPREFDRDFRPTTNRVRTRWERIATAQ
                     RQGKPMPPIDVYRIDELHFVKDGHHRVSVARAMGFESIDAYVTEVLTVVRPDPGSSRI
                     DLPLKSHERLFRERVPLPPEDHARIDLSDEWRYAGLAEGVEAWGFRAMQARQEPMTRE
                     EVAKAWFDDEYVPVIETLREADLLGGGSETEAYIRVITLRFLTLGTHEWTEEVLERLR
                     GEIRRPSRGDDTMVRRLRKELR"
     gene            complement(2214..3317)
                     /locus_tag="VFY44_04475"
     CDS             complement(2214..3317)
                     /locus_tag="VFY44_04475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX5909725.1"
                     /translation="MSFDLDAALDNTVKIGASDLHVKVAACPRVRVAGRLVDLPGSRE
                     VTPEDAIAVRDRVITAPGKQAEMEEIGSTDSSYAAAGHRFRATAFTQRTFPSFVFRAV
                     PDAPDAETLGLPAAVAKWGGDVKRGLVVVTGPTGSGKSTTCAAVIDGINSTRDCHILT
                     IEDPIEFLHQDKRAIVCQREIGQDAPSYHVALRAAMRQDPDVIMVGEVRDEETAMTAL
                     RAAETGHLVLCTMHTIDASDTIQRFVDFFGEQRGPLARQMLAGTLVGVCSQRLIPAIK
                     GGMALNAEVLINSSRVQDLIVEGMSTSEVHKAIKEGDFYGMQSFDQSLLELVKEGSVS
                     QEDALSYASEPNDFRLSLQTVGAAGQYAGVTGS"
     gene            3620..>4832
                     /locus_tag="VFY44_04480"
     CDS             3620..>4832
                     /locus_tag="VFY44_04480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX5909726.1"
                     /translation="MTDGTVQYFVVDAPEGATLQDIRTLVPGEPIGFADVPPGSAPTT
                     PTTPTTPAPKPPESDFGELQDDNKPAKPGKQPNQKDPGTKRKPKPKVETQPEQVLKAP
                     VQPEGERKRKKRVRRRGATVRPETRGADGAPLPGNPGFVDALPGPSTAQGVPNFVIRK
                     FRVPVFLLPIYQAAGIQYGVRWEVLAAINEIETDYGRNLNVSSAGALGWMQFMPATWR
                     QYGTDANKDGKKDPYNPVDAIFAAARYLKAAGYEDDVRRSIFAYNHADWYVDSVMLRA
                     RLIAGVPADLVGSLTGLTEGRFPVAARARYADDLKEQKVKKARAGENAANVVEDQDGR
                     RSVEIFAKENSPVVATNDGVVKKIGRSEKLGRYLILQDVYGNRYMYAGLGEISQYYPV
                     PKSDPSGARTSL"
BASE COUNT          739 a         1784 c         1702 g          607 t
ORIGIN      
        1 ggtggccggc gggccgaagg cctcgacctc cagcgccagc gactcggccg gcgcgggcag
       61 ctcggccagc ttgggggtca gggccagggc gatccggccc gggtcggcgc tggcgtggcg
      121 cagcgtgacc gcgcagcgcc aggtgccccc ggccacgaac cgggccgcca cggccagcga
      181 gcgcatggtc cgcccgcgcc gctcgcgccg ggccagcgcg cgggcgatca gcagttccag
      241 cgcccgctcg agctgggggc cggaggccgc gtcgggcagg tcgatgcgct cggacaccgg
      301 ctcgggcggg cgccgcggga ccagcggggt gtcacggccc cgggccaggt ccagggccag
      361 caggccgggg tgaccgaagc gctcggccac cgcccgcgag ggcagcgccg ccaggtcgcc
      421 gagcgtgcgg atgcccagcc gttcgagcac gtccgggagc tcggccagct cgggccgcga
      481 gcgcagcaat gctgcgggca gcggggccag gaagcgcgtg gtctcgtcct ggcccaccac
      541 cacgggcgcc cgccgcggcc tggtctggcg ggcggcgcag tgggcggcga agcgcgactc
      601 ggccgccccc aggcgcgacc ccggcccgag cgcgcgccgg gtggcgtcca ggacgccggc
      661 caggtcgcca ccgtgcaggc cctcgagccc acccgccgcg aagaaggccg ctccggcgtg
      721 ctgggactcg ggcgcggcgc cgatcgactc gatccggtcc agcacctcgt gccacaggct
      781 gcgcacgccc tcgggatcgg gtggctccag gcgtagctcc gggcaccgcg acagcgcctc
      841 ccccacccgc atgccggcca cgatgccgaa cgcctccgcc ggcgccgaga cctcgccgat
      901 cgtctgctcg cggcccgcct cgggtgccag cgccaccggc tcggacagca gcgcgcgccg
      961 atcccccagg gccgccagga gggcgaatcg ggggatgagg acacacacga ccacgtacga
     1021 acatacgttc gcatacgggc gggccgcggc gcaaggtccc ttgcgcgcca ggtgggcacc
     1081 ccgccccgac acccggccgg cgaacgccgc gcggcacatt tctcccgccc agcgggagca
     1141 atccaccgcg cgcgaagaac cgcgtcacag aacgccgcaa cccgtgacgg gaccgttacg
     1201 gccctccgcc ctacccagcc gcagttttct cgccgagccc gcagggacgc cagacggaag
     1261 ccggcagagc cgagcacgag cccacgttgc gccccgccgt accctccaag gagggaatgg
     1321 cacgcgatac cgggatgccc gagaccgacg ccagcttcga cttcgcgcgc gaacggcgcc
     1381 ggcgggccgt cgggaagctc tcctcgacgc tgcgcctggc tcccgcggac gtgaacgtga
     1441 tcctgccctt cgacgaggtc gtcgaggcgc tggggcgccg gggcgagaag cggctggggc
     1501 tgaagtcgat cgcgctggac tcgatcgtgg gcaccgtgga ccgcccgcgc gagttcgacc
     1561 gcgacttccg ccccaccacc aaccgcgtcc gcacgcgctg ggagcggatc gccaccgcgc
     1621 agcgccaggg caagccgatg cccccgatcg acgtctaccg gatcgacgag ctgcacttcg
     1681 tcaaggacgg ccaccaccgc gtgtcggtgg cccgggcgat gggcttcgag tcgatcgacg
     1741 cctacgtgac cgaggtcctg accgtggtcc ggcccgaccc cgggtcgtcc cggatcgacc
     1801 tgccgctgaa gagccacgag cggctcttcc gcgagcgcgt gccgctcccc ccggaggacc
     1861 acgcgcggat cgacctctcc gacgagtggc ggtacgccgg cctggccgag ggcgtcgagg
     1921 cctggggctt ccgcgccatg caggcccgcc aggagccgat gacgcgcgag gaggtggcca
     1981 aggcctggtt cgacgacgag tacgtgccgg tcatcgagac gctccgcgag gccgacctgc
     2041 tgggcggcgg cagcgagacc gaggcctaca tccgggtgat cacgctgcgc ttcctgaccc
     2101 tgggcaccca cgagtggacc gaagaggtgc tcgagcgcct gcgcggcgag atccgccgcc
     2161 cctccagggg cgacgacacg atggtgcgcc ggctgcgaaa ggaactgcgc tagctaggag
     2221 ccggtgacgc cggcgtactg cccggccgcc ccgaccgtct gcagcgagag gcggaagtcg
     2281 ttgggctcgc tggcgtagct gagcgcgtcc tcctgggaga ccgaaccctc cttgaccagc
     2341 tcgagcaggc tctggtcgaa ggactgcatc ccgtagaagt cgccctcctt gatcgccttg
     2401 tgcacctcgg aggtggacat gccctcgacg atcaggtcct ggacccgcga gctgttgatc
     2461 agcacctcgg cgttcagcgc catgccgccc ttgatggccg ggatcagccg ctgcgagcac
     2521 acgccgacga gcgtgccggc cagcatctgg cgcgccagcg gcccgcgctg ctcgccgaag
     2581 aagtccacga accgctggat cgtgtccgag gcgtcgatgg tgtgcatggt gcacagcacc
     2641 aggtggccgg tctcggcggc gcggagcgcg gtcatggccg tctcctcgtc gcggacctcg
     2701 ccgaccatga tcacgtcggg gtcctggcgc atcgccgcgc gcagcgcgac gtggtagctg
     2761 ggggcgtcct ggccgatctc gcgctggcag acgatcgccc gcttgtcctg gtgcaggaac
     2821 tcgatcgggt cctcgatggt caggatgtgg cagtcgcggg tcgagttgat cccgtcgatc
     2881 acggccgcgc aggtggtgct cttgcccgac ccggtggggc cggtgaccac caccaggccg
     2941 cgcttgacgt cgccgcccca cttggccacg gcggcgggca ggcccagcgt ctcggcgtcc
     3001 ggcgcatcgg gcacggcccg gaacacgaac gaggggaagg tgcgctgggt gaaggcggtg
     3061 gcgcggaaac ggtgcccggc ggcggcgtag gacgagtcgg tcgagccgat ctcctccatc
     3121 tccgcctgct tgccgggagc ggtgatgacc cggtcgcgga cggcgatggc gtcctcgggg
     3181 gtcacctccc gggagccggg caggtcgacg aggcggcccg cgacccgcac gcgcgggcag
     3241 gccgcgactt ttacgtgcag gtccgaggcg ccgatcttga cggtgttgtc gagggccgca
     3301 tcgagatcga aggacatggg gtgccaatcg tccggaaggg ggcgcacctt gagggggcga
     3361 agagtgtttg cgtgaattgg gattcctgcg tgccgcggtc gcgcccttgc gcgcgagaag
     3421 aaccggcccc gccggtggtt acgctggatg cgaggctcaa gctggcggtc gcgccgccga
     3481 tacgcataga gagcacgtcc cgccccggat gatgaagcga gcgatccaca ccctctgcct
     3541 gctggcggcc cttgccgcag ccttggcgac cgcgctcacg atccccgcct ccgcggagaa
     3601 gcgcaacgtg gccgttcgta tgaccgacgg tacggtccag tacttcgtcg tggacgcgcc
     3661 cgagggcgcg accctccagg acatccgcac cctggtcccg ggcgagccga tcggcttcgc
     3721 ggacgttccc ccgggctccg cgcccacgac ccccaccacg ccgaccacgc ctgctcccaa
     3781 gcctccggag agcgatttcg gcgagctgca ggacgacaac aagcctgcaa agcccggaaa
     3841 acagccgaac cagaaggatc ccggcaccaa gcgtaagcct aagcctaagg tcgagactca
     3901 gccggagcag gtccttaagg ctccagtaca gcctgagggt gagcgaaagc gcaagaagag
     3961 ggtgcgccgc cgcggcgcga ccgtccgtcc ggagacccgc ggcgccgacg gcgcccccct
     4021 ccccggcaac cccggcttcg tcgacgccct ccccgggccc tccaccgccc agggcgtccc
     4081 caacttcgtg atccgcaagt tccgggtgcc ggtgttcctg ctgccgatct accaggccgc
     4141 cgggatccag tacggcgtcc gctgggaggt gctggcggcc atcaacgaga tcgagaccga
     4201 ctatggccgc aacctgaacg tctcctccgc cggcgcgctg ggctggatgc agttcatgcc
     4261 cgcgacctgg cgccaatacg gcaccgatgc caacaaggac ggcaagaagg acccctacaa
     4321 cccggtcgac gccatcttcg ccgccgcccg ctacctcaag gccgccggct acgaggacga
     4381 cgtccgccgc tcgatcttcg cctacaacca cgccgactgg tacgtggact ccgtgatgct
     4441 gcgcgcccgc ctgatcgccg gcgtgcccgc cgacctggtc ggctcgctga ccggcctgac
     4501 cgagggccgc ttccccgtcg ccgcccgcgc ccgctacgcc gacgacctca aggagcagaa
     4561 ggtcaagaag gccagggccg gcgagaacgc cgccaacgtc gtggaggacc aggacggccg
     4621 ccgctcggtc gagatcttcg ccaaggagaa ctccccggtc gtggccacca acgacggcgt
     4681 cgtgaagaag atcgggcgca gcgagaagct cggccgctac ctgatcctcc aggacgtgta
     4741 cggcaaccgc tacatgtacg ccggcctcgg cgagatctcc cagtactacc ccgtgccgaa
     4801 gtccgacccc tcgggcgctc gcaccagcct ga
//
LOCUS       DASUPW010000195         4075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1017843, whole genome shotgun sequence.
ACCESSION   DASUPW010000195 DASUPW010000000
VERSION     DASUPW010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017843"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="VFY44_04485"
     CDS             complement(<1..1048)
                     /locus_tag="VFY44_04485"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX5909727.1"
                     /translation="MREAVIVDAVRTPIGRGKPGKGGLNPIHPVDLLAHSLREIVDRT
                     GIDPERIDDVIGGCVDQVNEQAMNVTRSAVLAAGLPETVPATTVDRQCGSSQQAVHFG
                     AQGVMAGAYDIVIAAGVESMSRVPMWSNVTNGADPFGPGVAARYPDGLVPQGISAELI
                     AAKWELPRSELDRFSMESHQRAAAAHADGAFDDELIAIDGLDTDESIRPGTTLETLAG
                     LKPAFEDPAAKERFPQIDWKVTAGNSSPINDGSSAVMITTPEIAKSLGLEPKARLHAF
                     SVAGDDPVMMLTGVIPATAKVLEKAGMTIEDIDLFEVNEAFASVVLAWQRETGADLSK
                     VNVAGGAIALGHPLG"
     gene            1114..1716
                     /locus_tag="VFY44_04490"
     CDS             1114..1716
                     /locus_tag="VFY44_04490"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5909728.1"
                     /translation="MATETEETREDGRRTRGRKTRASILDMAADLASVDGLEGLSIGR
                     LAVATGMSKSGLFAHFGSKEELQVATYFHARERFLAEVVLPPREFEPGRERVDALVEC
                     WLRYMRTEVFRGGCFFSEVRTEFGSREDGPVRRVVVKGTLRWLDLLARELDALPGDRD
                     VEQLAFEIDALGTAANYRFQLLQDPAVFDQAAAAISARLG"
     gene            complement(1723..3159)
                     /locus_tag="VFY44_04495"
     CDS             complement(1723..3159)
                     /locus_tag="VFY44_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5909729.1"
                     /translation="MPDAADFVAACSRARDAQRDWAATPAPRRGRAIQQIGRIVEANK
                     EALARVMTAEMGKPIAESRGEVQEVIDTADFFLGEGRRLYGQTVPSEMADKQLFTFRN
                     PVGVAAIITAGNFPTAVPAWYLIPALLCGNAVVWKPAEYTPEIGRALTSLFQAGGLPE
                     GVLNTVIADGPATFEGLEQALGQGLVDKVGFTGSSAVGSKIGELCGRHLQNPCLELGG
                     KNPLVVMDDADLDLAVEGALFSGFGTAGQRCTSMGTAIVHESVHDDFLARFTTATEGA
                     VVGDPFEDVLYGPMINQRFADRFEEWLGLVEDHHDVSGSSATGRITADNPRDGFVGDP
                     AEGLYFHPTIVAGVTAGDAIYSTETFGPIVGVARFGDFDEAVELANGHGYGLSAAIYT
                     SDAAHALKFRQRVSAGMLSVNNSTSGAEAHLPFGGNGKSGNGSRLSGMWVLDQFTRWQ
                     SMNWDYAGKLQKAQMDIDDVEADLDFRL"
     gene            complement(3170..>4075)
                     /locus_tag="VFY44_04500"
     CDS             complement(3170..>4075)
                     /locus_tag="VFY44_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5909730.1"
                     /translation="GARGVLDPSHPSWLGLPPHLPEVGALWDYADLVVSVGSDLDGMM
                     TQNFAQPQPPKMVALNVDAGDAVKNYAADVVVEGDVGRTARALAGELPPRADPPSLAA
                     LRTDTLDRVREDEPEGTAFLAEMAQAIDPGTAVFADMCIPGYWLASMHPFARPRRLAY
                     PVGWGTLGFAFPASIGAALGQDDPVLCVCGDGGFMFAAGELAAVAQERAPLTILVVDD
                     GGYGMLRFDQTKAATEPFGVDLVTPDWSRLAGAFGIECEEAAIGAGLADVLRRRLASR
                     EPSMVVVRTELPPPPSTSPRWYRRA"
BASE COUNT          549 a         1455 c         1479 g          592 t
ORIGIN      
        1 ccccgagcgg atggccgagg gcgatggcgc cgccggccac gttgaccttg gacaggtcgg
       61 cgcccgtctc gcgctgccag gccagcacca cgctggcgaa ggcctcgttg acctcgaaca
      121 ggtcgatgtc ctcgatcgtc atcccggcct tctccagcac cttggccgtg gcggggatca
      181 cgccggtgag catcatcacc gggtcgtcgc cggccaccga gaacgcgtgc aggcgcgcct
      241 tgggctccag ccccagcgac ttcgcgatct cgggcgtggt gatcatcacg gccgagctgc
      301 cgtcgttgat gggggaggag ttgcctgcgg tgaccttcca gtcgatctgg gggaaccgct
      361 ccttcgcggc cgggtcctcg aacgccggct tgagcccggc cagggtctcc agggtggtgc
      421 ccggccggat cgactcgtcg gtgtccaggc cgtcgatggc gatcagctcg tcgtcgaacg
      481 caccgtcggc gtgcgcggcc gcggcacgct ggtggctctc catcgagaac cggtcgagct
      541 cggaccgcgg cagctcccac ttggcggcga tcagctcggc gctgatgccc tggggcacca
      601 ggccgtcggg gtagcgggcg gccacgccgg ggccgaacgg gtcggcgccg ttggtcacat
      661 tcgaccacat gggcacgcgg ctcatgctct ccacgccggc ggcgatcacg atgtcgtagg
      721 cgccggccat gacgccctgc gcgccgaagt ggacggcctg ctgggacgac ccgcactggc
      781 ggtcgaccgt cgtggcgggc accgtctccg gcagcccggc ggccagcacg gccgagcggg
      841 tcacgttcat cgcctgctcg ttgacctggt ccacgcagcc gccgatcacg tcgtcgatgc
      901 gctcggggtc gatgccggtg cggtccacga tctcgcgcag cgagtgggcc agcaggtcca
      961 cggggtggat ggggttgagg ccgcccttgc cgggcttgcc acgcccgatc ggtgtgcgga
     1021 cggcgtcgac gatcactgct tcacgcatct gaggctcctt tgtacgaacg gtcgttcttt
     1081 tagattagag cacgatcgtg ctactttcgt cgagtggcca ccgagaccga ggagaccagg
     1141 gaggacgggc gccggacgcg cggccgcaag acgcgcgcat cgatcctgga catggccgcc
     1201 gacctggcat ccgtcgacgg cctcgagggg ctgagcatcg gccggctggc ggtggccacc
     1261 ggtatgagca agagcggcct gttcgcccac ttcggctcca aggaggagct gcaggtggcc
     1321 acgtacttcc acgcccgcga gcgcttcctg gccgaggtgg tcctgccccc gcgcgagttc
     1381 gagcccggcc gcgagcgcgt ggatgccctg gtcgagtgct ggctgcgcta catgcgcacc
     1441 gaggtcttcc ggggcggctg cttcttcagc gaggtgcgca ccgagttcgg ctcgcgcgag
     1501 gacgggccgg tgcgccgggt cgtggtgaag gggacgctgc gctggctgga cctgctggcc
     1561 cgtgagctgg acgcgctgcc gggcgaccgg gacgtggagc agctggcgtt cgagatcgac
     1621 gcgctgggca ccgccgccaa ctaccgcttc cagctgctcc aggacccggc cgtgttcgac
     1681 caggccgccg ccgcgatcag cgcgcggctg ggctgacgcc ggctagagcc ggaagtccag
     1741 atcggcctcg acgtcgtcga tgtccatctg ggccttctgg agcttgccgg cgtagtccca
     1801 gttcatcgac tgccagcggg tgaactggtc cagcacccac atgcccgaaa gccgcgagcc
     1861 gttgccggac ttgccgttgc cgccgaaggg caggtgcgcc tcggcgcccg aggtggagtt
     1921 gttcaccgac agcatccccg cgctgacgcg ctggcggaac ttcagcgcgt gggcggcatc
     1981 gctggtgtag atcgccgccg acagcccgta gccgtggccg ttggccagct cgacggcctc
     2041 gtcgaagtcg ccgaagcgag cgacgcccac gatgggcccg aaggtctcgg tggagtagat
     2101 ggcgtcgccg gcggtcacgc ccgcgacgat cgtggggtgg aagtagaggc cctcggcggg
     2161 atcgccgacg aacccgtcgc gggggttgtc ggccgtgatc cgcccggtgg ccgaggagcc
     2221 ggacacgtcg tggtggtcct ccaccagccc cagccactcc tcgaagcggt cggcgaagcg
     2281 ctggttgatc atcggcccgt acaggacgtc ctcgaacggg tcgccgacca cggcgccctc
     2341 ggtcgcggtg gtgaagcggg ccaggaagtc gtcgtggacc gactcgtgca cgatcgcggt
     2401 gcccatcgac gtgcagcgct ggcccgcggt gccgaagccg ctgaacagcg cgccctccac
     2461 ggccaggtcc aggtcggcgt cgtccatgac caccagcggg ttcttgccgc ccagctccag
     2521 gcacgggttc tgcaggtggc gcccgcacag ctcgccgatc ttcgagccga cggcgctgga
     2581 gccggtgaag ccgaccttgt ccaccagccc ctggccgagc gcctgctcga gcccctcgaa
     2641 ggtggcgggg ccgtccgcga tcacggtgtt cagcacgccc tcgggcaggc cgccggcctg
     2701 gaacaggctg gtcagcgccc ggccgatctc cggcgtgtac tcggcgggct tccacaccac
     2761 cgcgttgccg cacagcagcg cggggatcag gtaccaggcg ggcacggccg tcgggaagtt
     2821 gccggcggtg atgatggccg ccacgccgac cgggttacgg aacgtgaaca gctgcttgtc
     2881 ggccatctcc gacggcacgg tctggccgta caggcgccgg ccctcgccca ggaagaagtc
     2941 ggcggtgtcg atgacctcct ggacctcgcc gcgcgactcg gcgatgggct tgcccatctc
     3001 ggcggtcatc acccgcgcca gggcctcctt gttggcctcc acgatgcggc cgatctgctg
     3061 gatcgcgcgg ccgcgccggg gcgcgggggt ggcggcccag tcgcgctggg cgtcgcgggc
     3121 gcgggagcag gcggctacga agtcggcggc atcgggcatg gcacggactc tacgcccgcc
     3181 ggtaccaccg tggcgacgtg ctgggcgggg gcggcagctc ggtgcgcacc accaccatcg
     3241 acggctcgcg cgaggccagc cggcggcgca gcacgtcggc caggcccgcg ccgatcgcgg
     3301 cctcctcgca ctcgatcccg aacgcgccgg ccagccggct ccagtcgggc gtgaccaggt
     3361 cgacgccgaa cggctcggtg gcggccttcg tctggtcgaa gcgcagcatt ccgtagcccc
     3421 cgtcgtccac gaccaggatg gtcagtggcg cccgttcctg ggccaccgcc gccagctcgc
     3481 cggccgcgaa catgaagccg ccgtcgccgc acacgcacag caccgggtcg tcctgcccca
     3541 gcgcggcgcc gatcgacgcg gggaaggcga agcccagcgt gccccagccc accgggtagg
     3601 ccagccgccg cgggcgtgcg aacgggtgca tcgacgccag ccagtagccg gggatgcaca
     3661 tgtcggcgaa gacggcggtg ccggggtcga tcgcctgcgc catctccgcc aggaacgccg
     3721 tgccctcggg ctcgtcctcc cgcacgcggt ccagggtgtc cgtgcggagg gcggccagcg
     3781 acggcgggtc ggcccggggc ggcagctcgc ccgccagcgc ccgcgcggtc cggcccacgt
     3841 cgccctcgac caccacgtcg gccgcgtagt tcttgaccgc gtcgcccgcg tccacgttca
     3901 gcgccaccat cttcggcggc tggggctggg cgaagttctg ggtcatcatc ccgtccaggt
     3961 cactgcccac ggacacgacc aggtcggcgt agtcccacaa cgctcccacc tcgggcaggt
     4021 gcggcgggag gccgagccac gacgggtgcg acgggtccag gaccccccgc gcgcc
//
LOCUS       DASUPW010000196         2523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1710198, whole genome shotgun sequence.
ACCESSION   DASUPW010000196 DASUPW010000000
VERSION     DASUPW010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1710198"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..1183
                     /locus_tag="VFY44_04505"
     CDS             50..1183
                     /locus_tag="VFY44_04505"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX5909731.1"
                     /translation="MTARMPLALTAALSAAALVACGSESSEDSAAAPAPATDTRAEDT
                     AQADGSQAGAAANRPRTIATGLAVPWGIDFEPGRNGDALVAERTTGRIIRIARPGGKK
                     SVAHRIGGVATGSSEGGLLGLAVSPTYSRDKLVYAYYTRSGESENRIVRFKLGGRPQT
                     VLTGLQAASIHNGGRIAFGPDGKLYATVGDAGDTSNAQNRSSQNGKILRMNPDGSVPA
                     DNPFGGSRVWSLGHRNPQGIDWDSKGRLWAAEFGQNTFDEVNLIQKGKNYGWPDVEGR
                     GDTQGGKFVNPKVTWRTSDSSPSGLAISKGRLFVAALGGEGLWRSRLSGANTSKPAKL
                     YAGRYGRIRTVVRAPDGSVWFSTSNRDGRGDPKRGDDRILRIR"
     gene            1246..>2523
                     /locus_tag="VFY44_04510"
     CDS             1246..>2523
                     /locus_tag="VFY44_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909732.1"
                     /translation="MKQAGAWALGALLAAVIALPADARGQGPFLPYQGPDTWRSPGGI
                     AVSPDGRNVYVSGRQGLLTLERNAVDGSLKLGTWRVDPYGGTGGGVLMAPDGSSVYVV
                     RQDESDAKPPVKAFRRAPDGGLTAIDVDDSRQRNIEPTGAGISPLGDRVFAPNSYRGI
                     GLYLRAPTGELRLTGEVPIPSPQGPSVHEPMVMDSAGRLYVGLGFETSEIGVFEPVGD
                     SIELVQRVPCFCGSRGMTITTSGRRLVVGAAWRALAFERDPVTGRLGPMVDGYGSGGG
                     LGWQARPLATHGQSMYMLDEDSVHETRFTDTRWQVLRTWRNGVGGANGLDDPRALAVS
                     PDGRNVYVASGSRYGDDGPPGTVAVFARDPATGALSFTSLFTGPHPEPGANLRINGGA
                     RYTNSRDVRVTARSVDKAGVVLSNDPGFLGSKYF"
BASE COUNT          361 a          912 c          921 g          329 t
ORIGIN      
        1 agggggagat gcgcctcacc cggtgaggcg cccagctacc gtccgtcgca tgaccgcgcg
       61 tatgccgctc gccctgaccg ccgccctctc cgccgccgcg ctcgtcgcgt gcgggagcga
      121 atcgtccgag gactccgcgg ccgcgccggc gcccgcgacg gacacccggg ccgaggacac
      181 cgcacaggcc gacggcagcc aggccggggc cgcggcgaac cgcccgcgca ccatcgccac
      241 cggcctcgcg gtcccgtggg gaatcgactt cgagccgggc cgcaacggcg acgcgctggt
      301 ggccgagcgc accaccggcc gcatcatccg gatcgcgcgc cccggcggga agaagtccgt
      361 ggcccaccgg atcggcggcg tggccaccgg cagcagcgag ggcggcctcc tgggcctggc
      421 cgtctcgccc acctactcca gggacaagct cgtctacgcc tactacacgc gcagcggcga
      481 gagcgagaac cggatcgtgc gcttcaagct gggcgggcgc ccccagacgg tgctgaccgg
      541 cctccaggcg gcgtcgatcc acaacggcgg ccggatcgcc ttcggccccg acggcaagct
      601 ctacgccacc gtcggcgacg cgggggacac gtccaacgcc cagaaccgca gctcgcagaa
      661 cggcaagatc ctgcggatga acccggacgg ctccgtgccg gcggacaacc ccttcggcgg
      721 ctcgcgcgtc tggtcgctgg gccaccgcaa cccgcagggc atcgactggg actccaaggg
      781 ccggctgtgg gcggccgagt tcggccagaa cacgttcgac gaggtgaacc tgatccagaa
      841 gggcaagaac tacggctggc ccgacgtcga gggccgcggc gacacccagg gcgggaagtt
      901 cgtgaacccg aaggtcacct ggcgcacctc cgactcctcc ccgagcggcc tggccatctc
      961 caagggccgc ctgttcgtgg cggcactggg cggcgagggg ctctggcgct cgcgcctgag
     1021 cggcgccaac accagcaagc cggccaagct ctacgccggc cgctacgggc gcatccgcac
     1081 cgtggtcagg gcgcccgacg ggtcggtctg gttctcgacc tcgaaccgcg acggccgcgg
     1141 cgatcccaag cgcggcgacg accggatcct gcgcatcagg taggtcgccg ggcgcgcggg
     1201 gaacccggat cggccgtatg ttggccgtgt gaccggaggg ggcaggtgaa gcaggcggga
     1261 gcctgggcgc tgggtgcgct cctggccgct gttatcgcct tgccggcgga cgcgcggggg
     1321 cagggcccgt tcctgcccta tcagggcccg gacacctggc gcagcccggg cgggatcgcc
     1381 gtgagccccg acggaaggaa cgtgtacgtc tccggccgtc agggactcct cacgctcgaa
     1441 cggaatgccg tggacggttc gctgaagctc ggtacgtggc gggtcgatcc gtacggcggt
     1501 accggtggcg gggtcctcat ggcgccggac ggttcctcgg tctacgtggt ccgccaggac
     1561 gagtcggatg cgaagccgcc ggtcaaggcc tttcgccgcg cgccggatgg cggcctgacg
     1621 gcgatcgacg tcgacgacag ccggcagcgc aacatcgagc ccacgggcgc gggcatctcg
     1681 cccctgggcg accgggtgtt cgcgccgaac tcctaccgcg ggatcggcct ctacctccgc
     1741 gctccgaccg gtgagctgcg gctgacgggt gaggtgccga tcccgtcgcc ccagggcccc
     1801 agcgtccatg agcccatggt catggactcg gccggccgcc tctacgtggg gctcgggttc
     1861 gagacctccg agatcggcgt cttcgaaccg gtcggtgact cgatcgagct cgtccagagg
     1921 gtgccgtgct tctgcgggtc gcgcgggatg acgatcacga cgagcgggcg ccggctggtg
     1981 gtgggcgcgg catggcgcgc gctcgccttc gagcgcgacc ccgtcaccgg tcgcctgggc
     2041 ccgatggtcg acgggtacgg atccggcggg ggcctgggct ggcaggcgcg gccgctcgcg
     2101 acccacggcc agtcgatgta catgctggac gaggacagcg ttcacgagac gcgcttcacc
     2161 gatacgcgct ggcaggtgct gcgcacatgg cggaacggag tcggcggcgc gaacgggctg
     2221 gacgaccctc gcgccctggc ggtctccccg gacggccgga acgtgtacgt ggcctcggga
     2281 tcgcggtacg gcgacgacgg ccccccgggg acggtcgccg tgttcgcccg cgatcccgcg
     2341 acgggcgccc tgagcttcac gtccctgttc accgggccgc accccgagcc cggcgccaac
     2401 ctgaggatca acgggggagc ccgctacacg aactcccgtg atgtccgcgt gacggccagg
     2461 tccgtcgaca aggccggtgt ggtgctgtcg aacgatcccg gcttcctcgg ctcgaagtac
     2521 ttc
//
LOCUS       DASUPW010000197         4902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1849748, whole genome shotgun sequence.
ACCESSION   DASUPW010000197 DASUPW010000000
VERSION     DASUPW010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1849748"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(279..1262)
                     /locus_tag="VFY44_04515"
     CDS             complement(279..1262)
                     /locus_tag="VFY44_04515"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909733.1"
                     /translation="MDPVLSVRAPLRISLGGGGTDLPSWYRSHGGFVLSAAIDRYVRV
                     RVSPASGPRFRLEHLYLEEVEDPAEVEHPILRAALARHWNGRPLDLVSISEVPPGTGL
                     GSSGAYTVCSIKALDVASGRDPSAAELAEAACDVELGDLGRTVGKQDQYAAAHGGVNA
                     FTFGQDGGVDVRRLALGPATRAAFDQRFLLFGTGGEQRSAADMLATQVERTLAGDDEL
                     RRNLDGAEELARSACAALEADEPDRLGELMNDQWALKRERLAGVPMDRVEVLRVAALE
                     AGAVGARLVGAGGGGYLLVCAADPEPVRAALTEARAPELTFGLDEHGCVTE"
     gene            1290..1907
                     /locus_tag="VFY44_04520"
     CDS             1290..1907
                     /locus_tag="VFY44_04520"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5909734.1"
                     /translation="MQLTSIDLAADPGAWAAAGFAVAEDAVTLGAVRVRLRGADAGRG
                     LTGWSFAAPPAGPLDGLPEPSAAPAPDPGGQAHPNGITGVDHVVAFSPDLERTVAALE
                     AAGLDLRRRREGPTGAGSARQAFFRVGEAILEVVEHPPRAREAGDADAPARLWGLALL
                     TPDIEATAAAAGDLIGAPKDAIQPGRRIATFTRDAALGAAVAVIQ"
     gene            2009..2200
                     /locus_tag="VFY44_04525"
     CDS             2009..2200
                     /locus_tag="VFY44_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909735.1"
                     /translation="MTATGVTVDRNPELEFQLLVDLPGQEPYRVAHRLVVSDRVRHRF
                     GAGRAVPVSVDAAGTLAIG"
     gene            2228..2644
                     /locus_tag="VFY44_04530"
     CDS             2228..2644
                     /locus_tag="VFY44_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909736.1"
                     /translation="MGLFKNMKDAMGQVPDAQAAAAAAQQQAGISGTDMGAGLQARGA
                     LEGQAHEQNRILSIGSPGSATIKSHVDTGEQVAGNPVWILEIEVTPEGGAAYTVQKRE
                     IISSVAMSGYADGTTMPCRIDPADSNTIAFGDKPFM"
     gene            2647..2910
                     /locus_tag="VFY44_04535"
     CDS             2647..2910
                     /locus_tag="VFY44_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909737.1"
                     /translation="MGYWVRVVLGVALIVGGVAVFGYSTYGLIDIGTCASGGPYVSAR
                     ECPEGTGTLIVSLFPATLVTLVGVGLFASRGGRATAPGLPPAA"
     gene            2947..3396
                     /locus_tag="VFY44_04540"
     CDS             2947..3396
                     /locus_tag="VFY44_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909738.1"
                     /translation="MGMFKDMKDMVGVVRSDELKELKRKADAQPKTSMMDGLKAANQA
                     MDTAAEYQAMAGAGGLAGANPFANANAYSQTTPGSATVTAIADTGTRINESPVVELDL
                     MVTVAGREPYPVKHRQVISPVVMANFQPGSVITVRVDPADQSKIIIG"
     gene            3413..3898
                     /locus_tag="VFY44_04545"
     CDS             3413..3898
                     /locus_tag="VFY44_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909739.1"
                     /translation="MRTLPPIGWLNVLLGVVLGVGGFAMGQPLTGGILLVSLVASGAF
                     MIWLGSGWDQPLDDAAELYKYGRPANGQVLAVEETLLSTDGTRTAKLRVQISPVNEST
                     YTSTRTVALPGGRVPAVGQQVTVKFDPNSKKNFVLLEENVQVKDHITASMEQFSTGLK
                     A"
     regulatory      4013..4173
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            4184..4750
                     /locus_tag="VFY44_04550"
     CDS             4184..4750
                     /locus_tag="VFY44_04550"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX5909740.1"
                     /translation="MKTTLRTGALAFAIALGAAAPAAAAPAVNSPSVPAARPAENAFP
                     VRGPFNWGQEAAAFGGPRAGRSHEGQDVMSRTGTPIVAVADGTVLETGNDGGRGNYLN
                     LYDEARKVTYVYMHMVSPAAAKPGQRVGAGQRLGGVGCTGSCFGDHLHFEVRAGRGGE
                     GAARDPRPLLEKWRAADRIPATLPPGQS"
BASE COUNT          709 a         1793 c         1806 g          594 t
ORIGIN      
        1 gccggtgttg aagccgagct tgaggtcgtg ggccatcggc cgaccctatc tcgacgccgc
       61 cgttctcgcc gcggggccgc accagtggct cggccgacgg ctgggccggg tagggcgcgg
      121 gacgctccgt tcgaagccgt cgccgaggga acagatctcg gcccgcgtcg tgcgaacgcg
      181 gtcgtgggtc cgcacatgcg cgtccacgcg cagatcctga cccgcgccgc gaaggcgacg
      241 gcgtgggccg agacatgcgc ggccgtcgcc ccgcgcccct actccgtcac gcagccgtgc
      301 tcgtccagcc cgaacgtcag ctcgggcgcg cgggcctccg tcaaggccgc gcgcaccggc
      361 tcggggtcgg cggcgcagac cagcaggtag ccgccgcccc cggcgcccac cagcctcgcg
      421 ccgaccgcgc cggcctccag cgccgccacg cgcagcacct cgacccggtc catgggcacg
      481 ccggccaggc gctcgcgctt gagcgcccac tggtcgttca tgagctcgcc gaggcgatcg
      541 ggctcgtcgg cctccagcgc ggcacaggcc gagcgggcca gctcctcggc cccgtccagg
      601 ttgcggcgca gctcgtcgtc gccggccagc gtgcgttcga cctgggtggc cagcatgtcg
      661 gccgccgagc gttgctcgcc gccggtgccg aagagcagga agcgctggtc gaacgcggca
      721 cgggtcgcgg ggccgagcgc cagccggcgc acgtccacgc cgccgtcctg gccgaaggtg
      781 aacgcgttca cgccgccgtg ggcggcggcg tactggtcct gcttgccgac ggtgcggccc
      841 aggtcgccca gctcgacgtc gcaggcggcc tccgccagct cggccgcgga cgggtcgcgc
      901 ccggacgcca cgtccagcgc cttgatcgag cacaccgtgt acgcgccgga cgagcccagc
      961 ccggtgccgg ggggcacctc gctgatggac accaggtcga gcgggcgccc gttccagtgc
     1021 cgggccaggg cggcgcggag gatcgggtgc tcgacctcgg ccggatcctc gacctcctcc
     1081 aggtacaggt gctccaggcg gaagcgcggc ccggaggcgg gggacacgcg cacgcgcacg
     1141 taccggtcga tggccgccga cagcacgaac ccgccgtggc tgcgatacca ggacggcagg
     1201 tcggtgccgc cgccgccgag ggagatgcgc aggggagcgc gcacggagag caccgggtcc
     1261 acgcgtgcgc atgatggact atgggcccga tgcagctgac ctccatcgac ctggccgccg
     1321 acccgggcgc ctgggccgcc gcgggcttcg cggtggccga ggacgccgtg accctgggcg
     1381 cggtgcgggt gcgcctccgc ggcgcggacg ccggccgggg gctgacgggg tggagcttcg
     1441 ccgcgccgcc cgccggtccc ctggacggcc tgcccgagcc gtcggccgcc cccgcgcccg
     1501 accccggcgg gcaggcccac cccaacggca tcaccggcgt cgaccacgtg gtggccttca
     1561 gcccggacct ggagcgcacg gtcgccgcgc tggaggcggc ggggctggac ctgcgccgcc
     1621 ggcgcgaggg accgaccggg gccggatcgg cccgccaggc cttcttccgc gtcggcgagg
     1681 ccatcctcga ggtggtcgag cacccgcccc gggcgcggga ggccggcgac gcggacgcgc
     1741 cggcgcggct gtggggcctc gccctgctga cgcccgacat cgaggcgacc gccgctgccg
     1801 cgggcgatct gatcggcgcg ccgaaggacg cgatccagcc cggacggcgg atcgccacgt
     1861 tcacgcgcga cgcggcgctg ggcgccgccg tcgccgtgat ccagtgacct gagactggac
     1921 tgagaccagc tcgatactgc gcggggcccg gggccgacct acgttggctt caccgatgga
     1981 cgtacgaggt gaagccacta tcgagagcgt gaccgcgacc ggggtgacgg ttgaccgcaa
     2041 ccccgagctc gagttccagc tgctcgtcga cctgccgggg caggagccct accgggtggc
     2101 ccacaggctg gtggtctccg accgcgtgcg acaccgcttc ggcgccggac gggcggtgcc
     2161 cgtgagcgtc gacgcggccg gaaccctggc catcggctga cccgagacac cacacgagga
     2221 gacacggatg gggctgttca agaacatgaa ggacgcgatg ggccaggtgc ccgacgcaca
     2281 ggcggcggcc gcggcggccc agcagcaggc cggcatcagc ggcaccgaca tgggcgccgg
     2341 gctccaggcc cgcggcgcgc tcgagggcca ggcgcacgag cagaaccgga tcctgtcgat
     2401 cggcagcccg ggctcggcca cgatcaagag ccacgtggac accggcgagc aggtggccgg
     2461 caaccccgtg tggatcctcg agatcgaggt cacgcccgag ggcggcgccg cctacacggt
     2521 ccagaagcgg gagatcatct cgtcggtggc catgagcggc tacgccgacg gcaccacgat
     2581 gccctgccgg atcgacccgg ccgacagcaa cacgatcgcg ttcggcgaca agccgttcat
     2641 gtagccatgg ggtactgggt gcgagtggtg ctgggggtcg ccctgatcgt gggcggcgtg
     2701 gccgtgttcg gctacagcac ctacgggctg atcgacatcg gcacctgcgc ctcgggcggg
     2761 ccgtacgtgt ccgcccgcga gtgcccggag ggcaccggca cgctgatcgt gtcgctgttc
     2821 ccggccacgc tcgtgacgct ggtcggcgtg gggctgttcg cctcgcgcgg cgggcgggcg
     2881 accgcacccg ggctcccacc ggcggcctga gacagagaaa cgaacgaagc agaaggagaa
     2941 cgcacgatgg ggatgttcaa ggacatgaag gacatggtcg gcgtggtgcg cagcgacgag
     3001 ctcaaggagc tcaagcgcaa ggccgacgcg cagccgaaga cgtcgatgat ggacgggctg
     3061 aaggcggcca accaggccat ggacacggcg gccgagtacc aggcgatggc cggcgccggc
     3121 gggctcgcgg gcgcgaaccc gttcgccaac gccaacgcgt acagccagac cacgccgggc
     3181 agcgccaccg tgaccgccat cgcggacacg ggcacgcgca tcaacgagtc gccggtggtg
     3241 gagctggacc tgatggtcac cgtggccggc cgtgagccgt acccggtcaa gcaccgccag
     3301 gtgatctcgc cggtggtcat ggccaacttc cagccgggct cggtcatcac ggtgcgcgtg
     3361 gaccccgcgg accagtccaa gatcatcatc ggctgaggcg atcggcatgt cgatgagaac
     3421 actccccccg atcggctggc tcaacgtcct gctgggcgtg gtgctcggcg tcggcggctt
     3481 cgccatgggc cagcccctga ccggcggcat cctgctggtc tcgctggtgg cctcgggcgc
     3541 gttcatgatc tggctgggca gcggctggga ccagccgctg gacgatgcgg ccgagctgta
     3601 caagtacggc cgcccggcca acggccaggt gctggccgtc gaggagaccc tgctgtccac
     3661 cgacgggacc cgcacggcca agctgcgcgt gcagatctcg ccggtgaacg agtccaccta
     3721 cacgtccacg cgcacggtcg cgctgcccgg gggccgcgtg ccggccgtcg gccagcaggt
     3781 gaccgtcaag ttcgacccca acagcaagaa gaacttcgtg ctgctggagg agaacgtcca
     3841 ggtcaaggac cacatcaccg cctccatgga gcagttcagc accgggctga aggcgtaggg
     3901 cgggcccggc ctcgccccgg ccccatacgg ggcggggccg gtcctgatgt gtcggagcgg
     3961 gaagattcct gcaaatcggg gggttcgcgg aggctcctgc taccttccca cgctgttctg
     4021 aaggccgagt ccccgccgtc atgcggccgg gaacggggga accaccggca cgcgaagtgc
     4081 gtgcccgggg cgaatcgccg ccagttccgg cggcgtagcg gcagaccgtc gcgagcccgt
     4141 cagctcacct cgtaggccgt ccccgacgca ctggagcaag aggttgaaga cgacgcttcg
     4201 cacgggcgcc ctggcgttcg ccatcgccct gggcgccgcc gcgcccgccg ccgccgcacc
     4261 cgcggtgaac tccccatccg tgccggccgc ccggccggcc gagaacgcct tcccggtccg
     4321 tggcccgttc aactggggcc aggaggcggc cgccttcgga ggtccccgcg cgggccgctc
     4381 gcacgagggc caggacgtga tgtcgcgcac cggcaccccc atcgtcgccg tggccgacgg
     4441 caccgtgctc gagaccggca acgacggcgg ccgcggcaac tacctcaacc tctacgacga
     4501 ggcccggaag gtcacctacg tgtacatgca catggtgtcc ccggccgccg ccaagcccgg
     4561 ccagagggtc ggtgccggcc agcggctggg cggcgtgggc tgcaccggct cctgcttcgg
     4621 cgaccacctg cacttcgagg tgcgcgccgg gcgcggcggc gagggtgccg cccgcgaccc
     4681 gcgccccctg ctggagaagt ggcgggccgc ggaccgcatc ccggccacgc tgccgccggg
     4741 ccagagctag gtaacagctc ggaaacctcc cggtagcagg cgtttcacag cggggtcacg
     4801 ctcacgccac atgaggccgt cccgcacgcg gtggcctgtg aggcatcgca ggcacgccca
     4861 cactcgtcag ccgccgccac ttcgtcggca tgggcggcat gg
//
LOCUS       DASUPW010000198         7005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1018395, whole genome shotgun sequence.
ACCESSION   DASUPW010000198 DASUPW010000000
VERSION     DASUPW010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018395"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1702
                     /gene="ftsH"
                     /locus_tag="VFY44_04555"
     CDS             <1..1702
                     /gene="ftsH"
                     /locus_tag="VFY44_04555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX5909741.1"
                     /translation="EYDVTPGGSGLFVQLLFSLLPILLIVGLLFFFMNQMQGGGNRVM
                     QFGKSKAKLVSKDTPKTTFADVAGADEAVEELHEIKEFLETPKKFQALGARIPKGVLL
                     YGPPGTGKTLLARAVAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKQNSPCII
                     FMDEIDAVGRHRGAGMGGGHDEREQTLNQLLVEMDGFTMTDNIILIAATNRPDILDPA
                     LLRPGRFDRQIVVDRPDRKGRAKILEVHTRGKPLAKVIDLDNLAAQTPGFTGADLQNL
                     VNEAALLSARQGKREIDHLQLEEGIMRVIAGPEKKTRVMSEKERRITAFHEMGHAIVA
                     HFLENTDPVHKISVISRGQALGYTISLPTEDKFLTSRAELQDTMAMTLGGRAAEELVF
                     QEVTTGASNDLEKVTASAKQMVMRFGMSEKLGPRVFGHDSGQPFLGREFSNEPDYSDD
                     VAREIDSEVRRLVEEAHQSARDILERYSEALHLTSEILLKRETIEREQFIDLLDGKSE
                     DEVFGPDEPIVPRGTPPAAPEVPARKPERAPSPLPRPGLAGGSADIRGLDAPEKPDLS
                     "
     gene            1746..2096
                     /locus_tag="VFY44_04560"
     CDS             1746..2096
                     /locus_tag="VFY44_04560"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5909742.1"
                     /translation="MSSPFDRYVAAWNAGDLDGWLDAHAPDAEYVTFAGPEPRTFAGH
                     DGLREAWAEGRANWEVFRFQVLGDEGGVLEVGFRGIEQVQGIELSGVLWFRVDERDGR
                     LARVWSALDANELP"
     gene            complement(2110..3102)
                     /locus_tag="VFY44_04565"
     CDS             complement(2110..3102)
                     /locus_tag="VFY44_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX5909743.1"
                     /translation="MFAALAVTGDAASATPQIESTRLDGAVAGKPVELRVRATDPQAP
                     VTGMVAGFGRGESGFGLSSCLPPDSAGVPFGPAAAPGGRTTLTAPHTYDTPGTRDVGA
                     RIMSAGCTGGQPSTLIRLRVPVVPAGQRPQPVQPQSTREVPLGVPFPALPGLGQIPAG
                     RVDLPVGLPVAGVTAACPGSYRRFRRTRRGERAARSALLCLLNAERAKRGLPRVRGNG
                     RLVRAANGHSRAMVTRRFFAHVGPGSIGLVDRLRRAGYIPARGGTWLVGENIGFGRGR
                     YTRPATMHRAWMNSTPHRAAMLERRFREVGFGIVSGRPYGGGGATFTADFGRRR"
     gene            complement(3292..4419)
                     /locus_tag="VFY44_04570"
     CDS             complement(3292..4419)
                     /locus_tag="VFY44_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX5909744.1"
                     /translation="MALLSRGVAVATVVLALGMAGGAQAAPLIDSTLLKGNAVTGRTI
                     ELQVRASDRQAPVTGMVVGFGSGESGYGLSNCLPPDYRGRSFGPVAAPGRKVTLAAPH
                     IYPRPGKRSVVANVISGGCSPGQSSTAQALQVEVVRPGEAPKPIITTPPISVPVGQLL
                     PELPGLGELPVGSVNVPALPVQLPSIPGLPTVPLPTVPLPTLPTLPTLPGLPGLPRAT
                     ATAKCPHRNRWYSRSRAGERRAKAALLCLLNAERARRKLRPLRANARLARAARGHSRD
                     MVRRRYFAHIGAGASTPTKRLYAARYLPGAGRWAVGENIGFGTGAMTRPASIHSAWMH
                     STPHRAAMLSTRFREVGFGIYPGIPFQSRGATFTADFATTR"
     gene            4511..5329
                     /gene="folP"
                     /locus_tag="VFY44_04575"
     CDS             4511..5329
                     /gene="folP"
                     /locus_tag="VFY44_04575"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX5909745.1"
                     /translation="MDPVARVMGVVNVTPDSFSDGGRFLDPAAAVAHGAELAAQGAAI
                     LDVGGESTRPGAEPVGQDEELRRVVPVVRALAERLPGVTVSVDTTKAEVARQAVAAGA
                     AVVNDVSALRGDPELVDVVAQTGAGCCLMHMLGEPRTMQDDPRYGDVVSEVKAFLEER
                     LAFAVRHGVDERSVWLDPGIGFGKTARHNLELLARLDEIVALGRPVVVGTSRKTFLGS
                     LAGGRDPDHRLPGTIATNVMALERGAAIFRVHDVAAVVDALAVAGATVAAMRRG"
     gene            5322..5735
                     /gene="folB"
                     /locus_tag="VFY44_04580"
     CDS             5322..5735
                     /gene="folB"
                     /locus_tag="VFY44_04580"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX5909746.1"
                     /translation="MADDLDDDAFDEEEGAFEPTVTVAIAGLSLYTRHGVSDAERELG
                     QRLVFDIEFELTDCDAMVTDRVEDTVDYGEVCEQVALAAQERSYKTLERLCAAVADRL
                     MERYDAQSLSVKATKPEPPIPLPVDEVSVEVLREA"
     gene            complement(5751..6332)
                     /locus_tag="VFY44_04585"
     CDS             complement(5751..6332)
                     /locus_tag="VFY44_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909747.1"
                     /translation="MTALAPAVLLSAAVLLAAGAGFGGLSSLGQVADGPTLPSVDGIQ
                     ASGRSRADAPPASPVAALAAQTPATASGSGAAGPAAAGGGQGPGQTRANAPVGNPQRP
                     ATTSPGVRTPAQTPSGTAPANPSAPSTGSAPSTPNTPTQNAPVTPQLPSTPQLPSTPA
                     PVQQLLDLPRNLPAPVGPLTDRLLDTVTGIAPR"
     gene            6387..>7005
                     /locus_tag="VFY44_04590"
     CDS             6387..>7005
                     /locus_tag="VFY44_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909748.1"
                     /translation="MVEPGPSPGTDHPASRRRRSLARSLTLALVGLTLVLALAAAAGI
                     GGIYSARQDYEDSLARAYSLEAASAALFSAGVVEQAALQSTGRAGADLRRRARAAYRD
                     RARRARVLAAGDPASRRLVDRAVAGQERVVRLSRGPSRSARARARLTRRALVQGQRAE
                     TALTARQAVRRAEARDRTRDDTRSATLIAGGAGLLALLGAVALVVS"
BASE COUNT          934 a         2557 c         2648 g          866 t
ORIGIN      
        1 cgagtacgac gtcacccccg gcggcagcgg gctgttcgtg cagctgctgt tcagcctgct
       61 cccgatcctg ctcatcgtcg gcctgctgtt cttcttcatg aaccagatgc agggcggcgg
      121 caaccgcgtc atgcagttcg gcaagagcaa ggccaagctc gtcagcaagg acaccccgaa
      181 gacgaccttc gcggacgtgg ccggcgccga cgaggccgtc gaggagctgc acgagatcaa
      241 ggagttcctc gagaccccga agaagttcca ggcgctcggc gcccggatcc cgaagggcgt
      301 cctgctgtac gggccgccag gcaccggcaa gacgctgctg gcccgtgccg tggccggcga
      361 ggccggcgtg ccgttcttct ccatctccgg ctcggacttc gtcgagatgt tcgtgggcgt
      421 cggcgcctca cgcgtgcgtg acctgttcga gcaggccaag cagaactccc cctgcatcat
      481 cttcatggac gagatcgacg ccgtcggccg ccaccgcggc gccggcatgg gcggcggtca
      541 cgacgagcgc gagcagaccc tgaaccagct cctggtggag atggacgggt tcacgatgac
      601 cgacaacatc atcctgatcg cggccaccaa ccggcccgac atcctggatc ccgcgctgct
      661 gcgccccggt cgcttcgacc gccagatcgt ggtcgaccgc cccgaccgca agggccgcgc
      721 caagatcctc gaggtccaca cccgcggcaa gccgctggcc aaggtcatcg acctggacaa
      781 cctggccgcc cagacgcccg gcttcaccgg cgccgacctg cagaacctgg tcaacgaggc
      841 cgccctgctc tcggcccgcc agggcaagcg cgagatcgac cacctccagc tggaggaggg
      901 gatcatgcgc gtgatcgccg gcccggagaa gaagacccgg gtcatgagcg agaaggagcg
      961 ccggatcacc gcgttccacg agatgggcca cgccatcgtc gcccacttcc tggagaacac
     1021 cgacccggtg cacaagatct ccgtgatctc ccgcggccag gcgctgggct acacgatctc
     1081 gctgcccacc gaggacaagt tcctgaccag ccgcgccgag ctccaggaca ccatggccat
     1141 gaccctgggc ggccgcgccg ccgaggagct ggtcttccag gaggtcacca ccggcgcctc
     1201 gaacgacctc gagaaggtca ccgcgtccgc caagcagatg gtcatgcgct tcggcatgtc
     1261 cgagaagctc ggcccgcgcg tgttcggcca cgacagcggc cagccgttcc tgggccgcga
     1321 gttctccaac gagcccgact actccgacga cgtggcgcgc gagatcgaca gcgaggtccg
     1381 ccgcctggtc gaggaggccc accagtcggc ccgcgacatc ctggagcgct actccgaggc
     1441 gctgcacctg acctcggaga tcctgctcaa gcgcgagacc atcgagcgcg agcagttcat
     1501 cgacctgctg gacggcaaga gcgaggacga ggtcttcggc cccgacgagc cgatcgtccc
     1561 gcgcggcacc ccgccggccg cccccgaggt cccggcgcgc aagcccgagc gggcgccgtc
     1621 gccgctgccc cgccccggcc tggccggagg ctccgcggac atccgcgggc tcgacgcccc
     1681 agagaagcct gacctctcct agcgggagga gcgcagccga tcgtcgatcg gcgagcaccg
     1741 caccgatgtc gtcccccttt gaccgttacg tcgccgcgtg gaacgcgggc gacctggacg
     1801 ggtggctgga cgcgcacgcg cccgacgccg agtacgtgac cttcgccggc cccgagccgc
     1861 gcacgttcgc cggccacgac gggctgcgcg aggcctgggc cgagggccgc gccaactggg
     1921 aggtgtttcg cttccaggtc ctgggcgacg agggcggcgt gctggaggtc ggcttccgcg
     1981 gcatcgagca ggtccagggc atcgagctca gcggcgtcct gtggttccgc gtcgacgagc
     2041 gcgacgggcg cctggcccgc gtctggtcgg cgctggacgc caacgagctg ccgtagcgcg
     2101 cggccgccgt cagcggcggc ggccgaagtc ggcggtgaac gtggccccgc cgcccccgta
     2161 cgggcgaccg ctgacgatgc cgaagccgac ctcgcggaag cggcgctcga gcatcgcggc
     2221 gcggtgcggc gtgctgttca tccacgcccg gtgcatggtc gcgggccgcg tgtagcgacc
     2281 gcggccgaag ccgatgttct cgcccaccag ccacgtaccg ccccgcgcgg ggatgtaccc
     2341 ggccctccgc agccggtcga ccagcccgat cgacccgggc ccgacgtggg cgaagaagcg
     2401 ccgcgtgacc atcgcccgtg agtggccgtt ggccgcgcgg accaggcggc cgttgccgcg
     2461 cacgcgcggg aggccgcgct tggcgcgctc ggcgttcagc aggcacagca gcgcgctgcg
     2521 ggcggcccgc tcccctctcc gggtccggcg gaaccgccgg taggagcccg ggcaggcggc
     2581 ggtcacaccg gccacgggca accccaccgg gaggtccacc cggccggccg ggatctgacc
     2641 cagccccggc agcgcgggga agggcacacc cagcggcacc tcacgggtcg actgcggctg
     2701 gaccggctgc ggcctctgcc cggccggaac cacgggcacg cgcagccgga tcagcgtcga
     2761 cggctggccg ccggtgcagc cggcggacat gatccgagcc ccgacatcgc gggtccccgg
     2821 cgtgtcgtag gtgtgcggcg cggtaagggt cgtgcggcct cccggcgcgg cggcgggccc
     2881 gaagggcacg ccggccgagt ccggcggcag gcagctgctc aggccgaagc cggactcacc
     2941 gcggccgaag cccgcgacca tgcccgtcac cggcgcctgc gggtcggtgg cccggacccg
     3001 gagctcgacc ggctttccgg ccaccgcgcc gtccaggcgc gtcgactcga tctgcggcgt
     3061 cgccgacgcc gcgtcaccgg tcacggcgag cgccgcgaac acccccgctg cccctgcggc
     3121 cgccaaggcc gccctgcccg tcctgccagc atccatgctg tccccctaaa gtcccctgaa
     3181 gagcgattcc ctgctcggtg ggccaacgta ggtgcgatcc ccgatccgcg ccaacgggtg
     3241 caacgaaggc accggatcgg cgcggaaagc tcacggcaac ctgccgcccg ctcagcgcgt
     3301 ggtggcgaag tccgcggtga acgtggcccc gcgggactgg aacgggatcc cgggatagat
     3361 gccgaagccg acctcccgga agcgcgtgct gagcatcgcg gcgcggtgcg gcgtgctgtg
     3421 catccacgcg ctgtggatgc tcgccgggcg ggtcatggcg ccggtcccga agccgatgtt
     3481 ctcgccgacc gcccagcgcc cggcgcccgg gaggtagcgc gctgcgtaca ggcgcttggt
     3541 cggggtcgag gccccggccc cgatgtgggc gaagtaccgc ctgcgaacca tgtcgcgcga
     3601 gtggccccgg gccgcccgcg ccagcctcgc gttcgcgcgc agggggcgca gcttgcggcg
     3661 ggcgcgctcg gcgttcagca ggcacagcag cgcggccttg gccctgcgct cgccggcgcg
     3721 ggaccggctg taccagcggt tgcggtgcgg gcacttggcg gtcgcggtcg cccgcgggag
     3781 cccgggcagg cccggcaggg tcgggagcgt ggggagcgtg gggaggggca cggtgggcaa
     3841 cgggaccgtc ggcagcccgg gaatggacgg cagctggacc ggcagggcgg ggacgttgac
     3901 cgacccgacc ggcagctcgc ccagcccggg cagctcgggc aggagctggc cgacgggcac
     3961 gctgatcggc ggggtggtga tgatcggctt gggcgcctcg cccgggcgca cgacctccac
     4021 ctggagcgcc tgcgccgtgg acgactggcc cggcgagcag ccgccggaga tcacgttggc
     4081 gacgaccgag cgcttgcccg gcctggggta gatgtggggc gcggccagcg tgaccttgcg
     4141 gcccggtgcg gcgacggggc cgaacgagcg gccgcggtag tcggggggca ggcagttgct
     4201 caggccgtat cccgactcgc cgctgccgaa gccgaccacc atgccggtga cgggggcctg
     4261 gcggtccgac gcccgcacct ggagctcgat cgtgcgcccg gtcacggcgt tgcccttgag
     4321 cagggtcgag tcgatcaggg gggcggcttg cgcgccaccc gccatcccca gcgccaggac
     4381 gacggtcgcc acggccacac ctcgggacaa gagggccatt gcgccgaacc taacccacga
     4441 ggggatgctc gcgccaccca caagcgcagg aagcgggccg agcgggtcgg cgccatccat
     4501 actccgcccc atggatcccg tcgcccgggt catgggcgtg gtcaacgtga cgcccgactc
     4561 cttctcagac ggggggcggt tcctggaccc cgcggcggcg gtggcccacg gcgccgagct
     4621 cgcggcccag ggcgccgcca tcctggacgt cgggggcgag tccacccgcc ccggcgccga
     4681 gccggtgggc caggacgagg agctgcggcg cgtggtcccg gtggtgcgcg cgctggccga
     4741 gcgcctgccc ggcgtcacgg tctcggtcga caccacgaag gcagaggtgg cgcggcaggc
     4801 ggtggccgcc ggcgcggccg tggtcaacga cgtgtccgcc ctgcgcggcg acccggagct
     4861 ggtggacgtg gtggcccaga ccggggccgg ctgctgcctg atgcacatgc tcggagagcc
     4921 gcggacgatg caggacgacc cgcgctacgg cgacgtggtg tccgaggtca aggcgttcct
     4981 ggaggagcgg ctggccttcg cggtgcggca cggcgtggac gagcgctcgg tctggctcga
     5041 cccgggaatc ggcttcggca agaccgcccg ccacaacctg gagctgctgg cccggctgga
     5101 cgagatcgtg gccctgggcc ggcccgtggt ggtgggcacc tcgcgcaaga cgttcctcgg
     5161 cagcctggcc ggagggcgcg acccggacca ccgcctgccg ggcacgatcg ccaccaacgt
     5221 gatggcgctg gagcgcggcg cggccatctt ccgcgtccac gacgtggcgg ccgtggtcga
     5281 tgccctggcg gtggccggtg ctacggtcgc ggcgatgcgc cgtggctgac gacctggacg
     5341 acgacgcctt cgacgaggag gagggcgcgt tcgagcccac cgtcacggtg gccatcgccg
     5401 ggctctccct gtacacgcgg cacggtgtct cggacgccga gcgcgagctg ggccagcggc
     5461 tggtgttcga catcgagttc gagctgacgg actgcgacgc gatggtcacg gaccgggtcg
     5521 aggacacggt cgactacggc gaggtgtgcg agcaggtcgc gctggcggcc caggaacggt
     5581 cctacaagac gctcgagcgc ctgtgcgccg ccgtcgccga ccggttgatg gagcgctacg
     5641 acgcccagtc gctgagcgtc aaggccacca agccggagcc accgatcccg ttgcccgtgg
     5701 acgaggtctc cgtcgaggtg ctgcgggagg cctgaccgtg cggcgcggtc tcagcgcggt
     5761 gcgatgcccg tgaccgtgtc cagcaggcgg tcggtcagcg ggccgacggg cgcgggcagg
     5821 ttgcggggca ggtccagcag ctgctgcacc ggggccggcg tggacggcag ctggggcgtc
     5881 gacggcagct gcggcgtgac cggagcgttc tgggtggggg tgttcggggt ggacggcgcc
     5941 gagcccgtgg acggggccga cgggttcgcg ggcgcggtgc ccgacggcgt ctgcgcgggc
     6001 gtgcggacgc ccggcgaggt ggttgcggga cgctgcgggt tgccgacggg cgcgttcgcg
     6061 cgcgtctggc cggggccctg tccgcccccg gccgcggcgg gacctgccgc acccgacccg
     6121 gacgcggtgg cgggggtctg cgcggccagc gcggccaccg gtgaggccgg cggggcgtcg
     6181 gcgcgggagc gtccggacgc ctggatgccg tcgaccgacg gcagcgttgg cccgtcggcc
     6241 acctggccca gcgagctcag cccgccgaag cccgcgcccg cggccagcag gaccgcggcg
     6301 ctgagcaaca cggccggggc cagtgcggtc actcctgcgg ccttcgtggc ctgccgtcgc
     6361 caatccgtgg tcaactccac agggtcatgg tagagcccgg tccaagccct ggcaccgatc
     6421 atccggccag tcgccggagg cgatcgctgg cgcgcagcct gaccctggcg ctggtcggcc
     6481 tgaccctggt gctggccctg gcggccgccg cgggcatcgg cggcatctac tcggcacgcc
     6541 aggactacga ggactcgctg gcgcgcgcgt actcgctgga ggcggcctcg gccgccctgt
     6601 tctcggccgg cgtggtggag caggcggcgc tccagagcac cggccgcgcg ggcgccgacc
     6661 tgcgccgccg ggcgcgtgcc gcctaccgcg accgtgcccg ccgggcccgg gtgctggccg
     6721 cgggcgatcc ggccagccgc cggctggtgg accgcgccgt cgccggccag gagcgggtgg
     6781 tgcgcctgag ccgcggcccc tcgcgctcgg cgcgcgcgcg tgcgcgcctg acccgtcgcg
     6841 cgctcgtgca gggccagcgc gccgagaccg cgctgaccgc gcgccaggcc gtccggcgcg
     6901 ccgaggcccg cgaccggacg cgtgacgaca cgcgttcggc cacgctgatc gccggcgggg
     6961 cgggtctgct ggcgctgctg ggcgccgtcg cgctcgtggt gtcgc
//
LOCUS       DASUPW010000199         7747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1295339, whole genome shotgun sequence.
ACCESSION   DASUPW010000199 DASUPW010000000
VERSION     DASUPW010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7747
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295339"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VFY44_04595"
     CDS             complement(<1..904)
                     /locus_tag="VFY44_04595"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX5909749.1"
                     /translation="MPSWRDGESHKRARCREQNEATAEAAEGAFRCECGDRSCACTIA
                     MTPGEYSAVRGYPTHFAVTRNHENPESEQVIEERPGFSVVETVAGDATKLARRGDPRQ
                     RRRDARWTEPPAPKAPETVPIEHETTERVVRTVVLALPLAALVAGGWLAWGGALRWHD
                     LVVLAVTYTVTGLGITVGYHRLFTHRSFKTTRVVRAALAVLGSMAVEGPVTEWVATHR
                     KHHQFSDQEGDPHSPHTDHEPGFRGTVRGLVHAHVGWMFRGRDMANPARYAKDLQGDR
                     DLRFISRTFPLWVAVGMAIPFGLGV"
     gene            complement(897..1238)
                     /locus_tag="VFY44_04600"
     CDS             complement(897..1238)
                     /locus_tag="VFY44_04600"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5909750.1"
                     /translation="MVATEHLDNGIPVVAVTGELDLATAPALEEPLQAICDQATGPVA
                     VDLGGCGFIDLRGLHVLLSAQERLDRAQRTLMLITGNPSVLRILQVTRVDCLFEIHPS
                     RAAATGAYLDA"
     gene            1479..2078
                     /locus_tag="VFY44_04605"
     CDS             1479..2078
                     /locus_tag="VFY44_04605"
                     /inference="COORDINATES: protein motif:HMM:NF015799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEX5909751.1"
                     /translation="MSPEEFKSLRVLIANEREERLQTTTTMVAAMGHEVIARELQVGE
                     VAAATAEALPDVALVGLGDSTQHALDLIARIVQEAACPVVAILESNDPVFVNEAAKRG
                     IFAYVSAGDPAELQSALDIVLRRFGEFHSLEGAFGRRAMTERAKGILMERHGVGEREA
                     FELLRSHSRQTGRKLIEVAEAVATSYLLLPAPDKPAPEG"
     gene            complement(2083..2397)
                     /locus_tag="VFY44_04610"
     CDS             complement(2083..2397)
                     /locus_tag="VFY44_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909752.1"
                     /translation="MPTTQIRLRHTLPVLALTAAFAVGCGGETTDISAAVDDTNKNLQ
                     GAAKLDCADEIDGGEGTKFDCTLKGTKSGKTETVNLKIEKDGIAPVDEKDYVAALQKV
                     AG"
     gene            2456..3388
                     /gene="cysK"
                     /locus_tag="VFY44_04615"
     CDS             2456..3388
                     /gene="cysK"
                     /locus_tag="VFY44_04615"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX5909753.1"
                     /translation="MAAPTANLADTVGDTPMVTLTRVAPDCGAELVAKLEVYNPAGSV
                     KDRIGVAMIDAAEAEGRITPGKTTIVEATSGNTGIALAFVCAARGYDLVLAMPQGMSR
                     EREGLLRLYGAEVRITESMGGMDEAVAAAREIAAEKGDAFICDQFSNPANPDIHRRTT
                     AEEIWRDMDGNVDALVCGVGTGGTVTGAGGRLKELNPDLTVVAVEPKGSPVLSGGRPG
                     PHRIQGIGAGFVPPVLDRDILDEVITVSDDDAIATAQRCARTEGVLAGISCGAALTGA
                     IELGSRPEMARKRVVVVMPDSGERYVSAPFFNPA"
     gene            3442..4527
                     /locus_tag="VFY44_04620"
     CDS             3442..4527
                     /locus_tag="VFY44_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909754.1"
                     /translation="MLSRSLLGAAGVTLALALLPGSALSQTKTQGTNLSGPGAAASGA
                     EAFGCETRWQPGLAYPGYYQREPTGHTTCTAFSPGLSTNDTHLVPGPGTVSKVRVLSG
                     ANPAPLRIQIVKRIFQTNPNPPYQITDAQCCTGTGSESVTFQPSANGVTEVALNPPLK
                     VTTTPSTNGASGHHDIVAVSAVGPGNLPIMDLGENSLNAGIYSSTPTMQMFYPKVQTG
                     LQGQAQHDYANYIVAMNYDWAAEGAGAGGGGGGGGGGTGTKVAPIKLKSKSLRLKKGK
                     VKAKIACTAARNQACKGRARLRTATRKPKLLASKALNIKGGKSKTVTFSLSRKARKRV
                     PRKSNKVTLAVDLGAAGSFAKAMKLKR"
     gene            4538..5299
                     /locus_tag="VFY44_04625"
     CDS             4538..5299
                     /locus_tag="VFY44_04625"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEX5909755.1"
                     /translation="MTLSPQELERYSRQLLMDDWGGQTQERVRGSRAIVIGTGALGSP
                     VATYLAAAGVGTIGLVDHDRVELSNLHRQPLFFTPDVGAPKAELAAARLGLLNPEVAV
                     EPYPVELTAQNADAIVMGADVVVDCTDSFESRYVVNDACCAQGVPLVEAAVVAFEAQL
                     LSIRPGGSACYRCVFPTAPPPEARRGCREAGVLGAMAGVVGSLQALEALKLLSGVGTP
                     LTDTLLRIDGLTAEQTLVATSRRDDCPACGGTSSL"
     gene            5304..5942
                     /gene="cysE"
                     /locus_tag="VFY44_04630"
                     /pseudo
     CDS             5304..5942
                     /gene="cysE"
                     /locus_tag="VFY44_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017638459.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
     gene            6081..>7747
                     /locus_tag="VFY44_04635"
     CDS             6081..>7747
                     /locus_tag="VFY44_04635"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5909756.1"
                     /translation="MGELRLSWAERLRAQLPQGRTLPRESWRARHRAILILAGAHAVA
                     VPLYGIAQGFPVGHCLLEGSGIGLLVLVAWLAGRQDRTRLASSAASVSLMTASAMTVH
                     LSSGVTEAHFHFFVMIVVLTLYEDWAPFFVSLAYVLIHHGVGSIVDPEMLYSHGSQAQ
                     EHPLRFAVIHGGFVAAAGIAGVSTWRHNEHARERALAAEREADKLYRSVVETLNEGVA
                     LQGPDGSIRTANAAAARILGSTNERMVGKSSIRGHRDVIREDGTPWPEAEQPAQTTAR
                     TGEGLRDVVMGLRSPAGEVTWISVTTKPLQREADGWSVVTSVTDITERRRAQAELQRL
                     ADRDPLTGLLNRRRLEAELVGLLAEADRYEDTGAVVVLDLDNFKYVNDSLGHAVGDEL
                     ICSTAEALRRRLRETDTLARLGGDEFAVLLPRIDEESAATVTRSLLEVVRDEAVVNTD
                     DGPRRTTASAGIAMIEPGRVASEVMAHADMAMYRAKEQGKDGAVVFEPGADRQAQARA
                     GLSWAERIRSALAHDGLFLEAQPIVALGEAGGAARYELLVRMRTDDG"
BASE COUNT         1131 a         2768 c         2818 g         1030 t
ORIGIN      
        1 ccaccccgag cccgaagggg atggccatgc cgaccgccac ccacaggggg aacgtacggc
       61 tgatgaagcg aaggtcgcgg tcgccctgga ggtccttggc gtagcgcgcg gggttggcca
      121 tgtcccggcc gcggaacatc cacccgacgt gtgcgtgcac gagcccgcgg acggtgcccc
      181 ggaagcccgg ctcgtgatcg gtgtgggggc tgtggggatc gccctcctgg tcggagaact
      241 ggtggtgctt gcgatgcgtg gcgacccact cggtcaccgg gccctccacg gccatcgagc
      301 ccagcacggc cagcgcggcg cgcaccacac gcgtggtctt gaagctgcgg tgggtgaaca
      361 ggcggtggta gccgacggtg atgcccagcc cggtgaccgt gtaggtcacg gccaggacca
      421 cgaggtcgtg ccagcgcagc gcgccgcccc aggccagcca cccacccgcc acgagggccg
      481 ccagcggaag cgccaggacc acggtccgga ccacgcgctc ggtggtctcg tgctcgatcg
      541 ggaccgtctc gggcgccttg ggggccggcg gctcggtcca gcgggcgtcg cgccggcgct
      601 ggcggggatc gccccggcgg gcgagcttgg tggcgtcgcc cgcgacggtc tccaccacgg
      661 agaatcccgg cctctcctcg atcacctgct cgctctcggg gttctcgtgg ttgcgggtca
      721 cggcgaagtg ggtggggtat ccgcgcacgg cggagtactc gcccggggtc atcgcgatcg
      781 tgcaggcgca ggaccggtcg ccgcactcac agcggaacgc gccctcggcc gcctcggccg
      841 tggcctcgtt ctgctcccgg cagcgggcgc gcttgtgcga ctcgccgtcg cgccagctag
      901 gcatcgaggt aggcccctgt ggccgcggcg cgggagggat ggatctcgaa caggcagtcg
      961 acgcgggtga cctggaggat ccggagcacg ctcgggttcc cggtgatcag catcaacgtg
     1021 cgctgggcgc ggtccagccg ctcctgcgcg ctcagcagga cgtgcaggcc ccggaggtcg
     1081 atgaagccgc agcctccgag gtccacggcc accgggccgg tggcctggtc gcagatggcc
     1141 tggagcggct cctcgagtgc gggcgccgtg gccaggtcga gctctcccgt caccgcgacc
     1201 accggtatgc cgttgtccag gtgctccgtg gccaccacga agctcgacct cggcagcgtc
     1261 gggcccaaga cacagggcgt ggtgtgcatt tccatgatcc gtcctccgga catcgcggtg
     1321 gcgccggcca agggctgagg ccgacagtgc tgcgaccgga tgctgaggtc gggttcgcgc
     1381 ggaccgtgaa tctggggtcc gtgctctacc gagggtagcg cgactgcttg cgcccggcca
     1441 cccgcgctcc taatctgatc ggagaaagcg ttcccgcagt gagtcccgaa gagttcaagt
     1501 cgcttcgcgt cctgatcgcc aacgagcgcg aggaacgcct ccagacgacc acgaccatgg
     1561 tcgccgcgat gggccacgag gtcatcgccc gtgagctgca ggtgggcgag gtggcggccg
     1621 cgacggccga ggcgctcccc gacgtcgcgc tggtcgggct gggcgacagc acccagcacg
     1681 ccctggacct gatcgcgcgg atcgtgcagg aggcggcgtg cccggtcgtg gcgatcctgg
     1741 agagcaacga cccggtcttc gtgaacgagg cggcgaagcg ggggatcttc gcctacgtga
     1801 gcgccggcga cccggccgag ctgcagagcg cgctggacat cgtgcttcgc cgcttcggcg
     1861 agttccacag cctggagggc gcgttcgggc gccgggcgat gaccgagcgc gccaagggga
     1921 tcctgatgga gcgccacggg gtgggcgagc gcgaggcctt cgagctgctg cgctcccact
     1981 ccaggcagac cgggcgcaag ctgatcgagg tggccgaggc cgtggccacc agctacctgc
     2041 tgcttccggc gccggacaag ccggcgccgg aggggtgacg ggtcagccgg cgaccttctg
     2101 cagcgccgcg acgtagtcct tctcgtcgac gggcgcgatg ccgtccttct cgatcttgag
     2161 gttgaccgtc tcggtcttgc cgctcttggt gcccttgagc gtgcagtcga acttcgtgcc
     2221 ctcgccgccg tcgatctcgt cggcgcagtc cagcttggcg gccccctgga ggttcttgtt
     2281 ggtgtcgtcg acggccgcgc tgatgtcggt cgtctcgccg ccgcagccga cggcgaaggc
     2341 cgcggtcagg gcgaggacgg gaagggtgtg gcgcaggcgg atctgggtgg tgggcatggc
     2401 gcatggttcc tcgccgtgtt ccgaatgcct ttaagcaccg atacggttcg gcgccatggc
     2461 agcaccgacc gccaacctgg ccgacaccgt cggcgatacg cccatggtca ccctgacccg
     2521 cgtggctccc gactgcggtg ccgagctggt ggccaagctg gaggtctaca acccggccgg
     2581 cagcgtcaag gaccgcatcg gcgtggccat gatcgacgcg gccgaggccg agggcaggat
     2641 caccccgggc aagaccacga tcgtggaggc caccagcggc aacaccggga tcgcgctggc
     2701 cttcgtgtgc gccgcccgcg gctacgacct ggtcctggcc atgccgcagg gcatgagccg
     2761 tgagcgcgag gggctgctcc gcctctacgg ggccgaggtg cgcatcaccg agtccatggg
     2821 gggcatggac gaggcggtgg cggccgccag ggagatcgcg gccgagaagg gcgacgcgtt
     2881 catctgcgac cagttctcca accccgccaa ccccgacatc caccggcgca ccaccgccga
     2941 ggagatctgg cgcgacatgg acggcaacgt ggacgccctg gtctgcggcg tcggcaccgg
     3001 cggcaccgtg accggcgccg gcggaaggct gaaggagctg aacccggatc tgacggtggt
     3061 ggcggtcgag ccgaagggct cgccggtgct gtcgggcggc cgccccggcc cgcaccggat
     3121 ccaggggatc ggcgccggct tcgtcccccc ggtgctggac cgcgacatcc tggacgaggt
     3181 gatcaccgtc tccgacgacg acgccatcgc caccgcccag cggtgcgcgc gcaccgaggg
     3241 cgtgctggcg gggatctcgt gcggcgcggc gctgaccggg gccatcgagc tgggctcccg
     3301 cccggagatg gcccgcaagc gggtcgtcgt cgtcatgccc gactcgggcg agcgctacgt
     3361 gagcgcgccc ttcttcaacc cggcctagca cccccgctag acctggggcg ggctcacccg
     3421 atcaggtgta ttgctggcgc catgctctcg cgctccctgc tcggcgcggc cggcgtcacg
     3481 ctggccctcg cgctgcttcc cggctccgcc ctctcccaga ccaagaccca gggcaccaac
     3541 ctgagcgggc ccggggcggc cgcatcgggc gccgaggcgt tcggctgcga gacccgctgg
     3601 cagcccgggc tggcctaccc cggctactac cagcgcgagc cgaccggcca caccacctgc
     3661 accgccttct cgccgggcct gagcacgaac gacacgcacc tggtgccggg gcccggcacg
     3721 gtcagcaagg tccgcgtgct ctccggcgcg aaccccgctc cgctgcgcat ccagatcgtc
     3781 aagcgcatct tccagaccaa tcccaacccg ccgtaccaga tcaccgacgc ccagtgctgt
     3841 accggcacgg gcagcgagag cgtgaccttc cagcccagcg ccaacggcgt gaccgaggtc
     3901 gcgctgaacc cgccgctcaa ggtcacgacc acgccctcga ccaacggcgc gtccggtcac
     3961 cacgacatcg tcgcggtcag cgccgtcggc ccggggaacc tgccgatcat ggacctgggc
     4021 gagaactcgc tgaacgccgg catctactcc tcgacgccga cgatgcagat gttctacccc
     4081 aaggtccaga ccgggctgca gggacaggcg cagcacgact acgccaacta catcgtggcc
     4141 atgaactacg actgggccgc cgagggcgcg ggcgccggcg ggggcggtgg aggcggcggc
     4201 ggaggcaccg gcacgaaggt cgcgccgatc aagctcaaga gcaagtcgct caggctcaag
     4261 aagggcaagg tcaaggccaa gatcgcctgc accgccgcca ggaaccaggc gtgcaagggc
     4321 agggcccgcc tgcgcaccgc caccaggaag cccaagctgc tggccagcaa ggccctgaac
     4381 atcaagggcg gcaagtccaa gacggtcacg ttctcgctgt cgcgcaaggc gcgcaagcgc
     4441 gttcccagga agagcaacaa ggtcacgctg gcggtcgacc tcggcgccgc gggcagcttc
     4501 gcgaaggcga tgaagctgaa gcgctagaac tcacggcgtg accctctcgc cgcaggagct
     4561 cgagcgatac tcgcgccagc tcctgatgga cgactggggc gggcagaccc aggagcgggt
     4621 gcgcgggtca cgggcgatcg tgatcgggac cggggcgctg ggcagcccgg tggccaccta
     4681 cctggccgcc gcgggcgtgg gcaccatcgg gctggtcgac cacgaccggg tggagctgtc
     4741 gaacctgcac cgccagccgc tgttcttcac gcccgacgtg ggcgcgccca aggccgagct
     4801 ggccgccgcc aggctcgggc tgctgaaccc cgaggtcgcg gtcgagccgt acccggtcga
     4861 gctgaccgca cagaacgccg acgccatcgt catgggggcc gacgtggtgg tggactgcac
     4921 cgacagcttc gagagccggt acgtggtcaa cgacgcgtgc tgcgcgcagg gggtaccgct
     4981 ggtggaggcg gccgtggtgg ccttcgaggc ccagctgctg agcatccggc cgggcggatc
     5041 ggcgtgctac cggtgcgtgt tccccacggc gccgccgccc gaggcgcgcc ggggttgccg
     5101 cgaggcgggc gtcctgggag cgatggccgg ggtggtggga tcgctccagg cactcgaggc
     5161 cctgaagctc ctgagcggcg tcgggacgcc gctgaccgac accctcctgc gcatcgacgg
     5221 gctgaccgcc gagcagacgc tggtggccac ctcgcgccgg gacgattgtc ccgcctgcgg
     5281 cggtacgtca tccttgtgac ctgatgctcg gactcggaac cctggcccgg gtggcgcgcg
     5341 aggtgcgcgc cgacctgaca gcagcgcagg accgcgaccc cgcggcgcgc agcgtcggcc
     5401 gggccgagat cctcctgacc tacggcggcg tgcaggcgct cctggcccac agggtggccc
     5461 acgcgctgca caccaccggc gtgccgctgg ccccgcgcgt ggtggccaac accagccgcg
     5521 tggtcaccgg cgtcgagatc cacccggccg ccgacatcgg cgacgcgctg ttcatcgacc
     5581 acggcgccgg ggtggtcatc ggcgagaccg cccgcatcgg cgacaacgtg accctgtacc
     5641 agggcgtgac cctgggcggg accgggttcc agcgcggcaa gcgccacccg acggtgggtg
     5701 acgacgtcgt cgtgggctcc ggcgcgaagc tgctgggccc gatcgaggtg ggccattgcg
     5761 ccaagatcgg tgccaactcg gtggtcatcc acgacgtgcc ggccgattcc acggtggtcg
     5821 gcaaccccgg gcacccggtg cgggtcaacg ggcgcaggcc cgagggcccc gacgccgact
     5881 gggcccatct gcccgatccg gtcgccgacg cgctcaaggg gctggccagc cgcgtgaacg
     5941 agctcgaggg cctcgtggcc gagctgacgg gcaagaagcc cgagcgggcg gacgtgacgc
     6001 cgctgcaacg ccgccgccac ggcggcgatc ccgccggcgg ctgacctcaa gtaccgcgcg
     6061 cacgagccga tgagacccat atgggtgaac tccgcctgtc gtgggccgaa cgcctgcgcg
     6121 cccagctccc acaaggacga acgcttccac gcgagtcatg gcgggcccgc caccgcgcga
     6181 tcctgatcct ggccggcgcc cacgccgtgg ccgtgccgct ctacgggatc gcgcagggtt
     6241 tccccgtcgg gcactgcctg ctggagggct cgggcatcgg cctgctggtg ctcgtcgcct
     6301 ggctcgccgg ccgccaggac cggacccggc tggcctcgtc cgcggcctcc gtctcgctga
     6361 tgaccgcctc ggcgatgacc gtgcacctgt cctcgggcgt gaccgaggcg cacttccact
     6421 tcttcgtgat gatcgtcgtg ctgaccctgt acgaggactg ggcgccgttc ttcgtctcgc
     6481 tggcgtacgt gctgatccac cacggcgtgg gcagcatcgt cgacccggag atgctctaca
     6541 gccacggcag ccaggcgcag gagcacccgc tgaggttcgc cgtcatccac ggcgggttcg
     6601 tggccgccgc gggcatcgcc ggcgtgtcca cctggaggca caacgagcac gcgcgcgagc
     6661 gcgcgctggc cgccgagcga gaggccgaca agctctaccg gtccgtggtc gagacgctga
     6721 acgagggcgt ggcgctccag ggccccgacg ggtccatccg tacggccaac gccgccgcgg
     6781 cgcggatcct gggcagcacc aacgagcgga tggtcggcaa gagcagcatt cgcggccacc
     6841 gcgacgtgat ccgcgaggac ggcaccccct ggccggaggc cgagcaaccc gcacagacca
     6901 cggcacggac gggcgagggc ctgcgcgacg tggtcatggg gttgcgctcg cccgccgggg
     6961 aggtcacctg gatctcggtg accaccaagc ccctccagcg cgaggcggac ggctggtcgg
     7021 tggtcacctc ggtcaccgac atcaccgagc gccgccgcgc ccaggccgag ctccagcgcc
     7081 tggccgaccg cgacccgctg accggcctcc tgaaccgccg ccgcctcgag gccgagctcg
     7141 tgggcctgct ggccgaagcc gaccgctacg aggacaccgg cgccgtcgtg gtgctggacc
     7201 tggacaactt caagtacgtg aacgactcgc tgggccacgc ggtcggggac gagctgatct
     7261 gctccaccgc cgaggccctg aggcggcggc tgcgcgagac cgacacgctg gcccggctgg
     7321 gcggcgacga gttcgccgtc ctcctgcccc ggatcgacga ggaatcggcg gccacggtca
     7381 cccgctcgct gctcgaggtc gtgcgcgacg aggccgtggt caacaccgac gacggcccgc
     7441 gccggacgac ggcctcggcc ggcatcgcca tgatcgagcc cggccgcgtg gccagcgagg
     7501 tcatggccca cgcggacatg gccatgtacc gggccaagga gcagggcaag gacggggcgg
     7561 tggtgttcga gcccggcgcg gaccgccagg cccaggccag ggccggcctc tcgtgggccg
     7621 agcggatccg ctccgcgctg gcccacgacg ggctgttcct ggaggcccag ccgatcgtgg
     7681 cgctgggcga ggccggcggc gccgcccggt acgagctgct ggtgcggatg cgcaccgacg
     7741 acgggga
//
LOCUS       DASUPW010000200         2133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1295419, whole genome shotgun sequence.
ACCESSION   DASUPW010000200 DASUPW010000000
VERSION     DASUPW010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295419"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VFY44_04640"
     CDS             <1..421
                     /locus_tag="VFY44_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909757.1"
                     /translation="TVEVNHNSPAYYLNEDRYADVKVPRSATRDARPDWRVLDHAGRV
                     DWHDHRIHWMAKTRPGVVEDPDARTKVFDWSVPIRVDGRPGKVTGDLHWVPRPGGSLP
                     AAAVGGLAALVVAGGALVVVVRRRRRRAGPEDTGEAW"
     gene            415..>2133
                     /locus_tag="VFY44_04645"
     CDS             415..>2133
                     /locus_tag="VFY44_04645"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEX5909758.1"
                     /translation="MVTRRLLTAVVVLVLGLALAPEAGAHALVTSTSPTRGAALEKEP
                     ERVVLRFSEAVETSFGAVRVFDAKGRRVDTGATGRPSASSVSARLRPGLADGPYTATY
                     RVVSSDSHPVSGGFTFTVGESGGASAASVADLVDSGGPGMATTTAFQAVRAVGYAAIA
                     IVVGGLLFLLVVWAPGFRAVADGDDSWRRAAEAMTGRVRRLVVVAAGAGALAAVLGVV
                     LQGATASGTSFTGALDGQVLHDVLATRFGRAWTGRLAAFALVGLALTLPAAARRPVPR
                     WLLALPLGYLVVAPALSGHAGATDQLLLVPSDVVHVAAMSAWVGGLVMLVFALPRATR
                     ALAPPDRTAVLAQCVTRFSTVALVAVAALVVTGVIQSVVQLDAFSDLWSSAFGRAVLV
                     KTALLLVLLGLGAYNRQRTRPQLVERARAGETPGATGLRLRRAIRAEVALLAAVLVAA
                     GALVGYSPTAGSAAAGPFSANENLGPARLELTVDPARVGANEIHLYLFDRRTGAQYDR
                     AKELTVSASLPAKRIGPLSLAARKTGPGHYTVRGASLVPAGDWVLAVESRVSAFDAFD
                     AKVEVPV"
BASE COUNT          213 a          818 c          808 g          294 t
ORIGIN      
        1 cacggtcgag gtcaaccaca actcgccggc gtactacctc aacgaggacc gctacgccga
       61 cgtgaaggtg cccaggtcgg ccacgcggga cgctcgcccg gactggcggg tgctggacca
      121 cgccgggcgc gtcgactggc acgaccaccg catccactgg atggccaaga cccggccggg
      181 cgtggtcgag gaccccgacg cgcgcacgaa ggtcttcgac tggtcggtcc ccatccgcgt
      241 cgacgggcgg ccgggcaagg tcacgggcga cctgcactgg gtgccgcggc ccggtgggtc
      301 gctaccggcc gccgccgtcg gcgggctcgc ggcgctggtc gtggccggcg gcgcgctggt
      361 cgtcgtcgtc cgccgacgcc ggcggcgggc ggggccggag gacaccggag aagcatggtg
      421 acccgtcgcc tgctgaccgc cgtcgtcgtg ctcgtcctgg ggctggccct ggccccggag
      481 gccggggccc acgcgctggt gacctcgacc agccccaccc gcggcgccgc gctcgagaag
      541 gagcccgagc gggtggtact gcgcttctcg gaggccgtgg agaccagctt cggcgccgtc
      601 cgcgtgttcg acgccaaggg ccggcgcgtg gacaccggtg cgaccgggcg accctccgcg
      661 tccagcgtgt ccgcccggct gcgccccggg cttgccgatg gcccctacac cgccacctac
      721 cgcgtggtct cctcggactc gcacccggtc tcgggcgggt tcaccttcac cgtcggggag
      781 tccggcggcg cctccgcagc gtcggtggcc gacctggtgg acagcggcgg cccgggcatg
      841 gccacgacca ccgcgttcca ggcggtgcgc gccgtgggat acgcggccat cgccatcgtc
      901 gtcggcggcc tgctgttcct gctcgtggtc tgggccccgg gcttccgggc cgtggcggac
      961 ggcgacgact cctggcgccg ggccgccgag gcgatgaccg ggcgcgtgcg ccggctggtg
     1021 gtggtcgcgg ccggcgcggg cgcgctcgcg gccgtcctgg gggtggtgct ccagggcgcc
     1081 acggcctcgg gcaccagctt caccggcgcc ctcgacggcc aggtgctgca cgacgtgctg
     1141 gccacgcggt tcgggcgcgc ctggacgggc cgcctggccg cgttcgcgct cgtgggcctg
     1201 gcgctgacct tgccggccgc ggcccgccgc cccgtgccgc ggtggcttct ggcccttccc
     1261 ctgggctacc tggtcgtcgc gccggccctg tccgggcacg cgggcgcgac cgaccagctg
     1321 ctcctggtgc ccagcgacgt cgtccacgtg gccgcgatga gcgcatgggt cggcgggctc
     1381 gtgatgctgg tgttcgccct cccccgcgcc acccgtgcgc tggcgccccc cgaccgcacg
     1441 gccgtcctgg cgcagtgcgt gacccggttc tcgacggtcg cgctggtcgc cgtcgcggcg
     1501 ctcgtcgtca ccggcgtgat ccagtcggtg gtccagctcg acgcgttcag cgatctctgg
     1561 agcagcgcct tcggccgcgc cgtcctggtc aagaccgccc tgctgctcgt cctgctgggc
     1621 ctgggcgcgt acaaccgcca gcgcacgcgg ccgcagctgg tcgagcgggc cagggccggg
     1681 gagacgcccg gcgccaccgg gctccggctg cgccgggcca tccgggccga ggtcgccctg
     1741 ctggccgcgg tgctggtggc tgcgggcgcg ctggtcggct actcgccgac cgcgggcagc
     1801 gctgccgcgg gcccgttctc ggccaacgag aacctcggcc cggcccggct cgagctgacc
     1861 gtggacccgg cgcgggtcgg ggccaacgag atccacctct acctgttcga ccgccgcacc
     1921 ggagcgcagt acgaccgggc gaaggagctg accgtctcgg cctcgctgcc cgcgaagcgc
     1981 atcggaccgc tgtcgctggc ggccaggaag acggggcccg gccactacac cgtgcgcggc
     2041 gcctcgctgg tgcccgccgg cgactgggtc ctcgccgtag agtcgcgcgt cagcgcgttc
     2101 gacgccttcg atgccaaggt cgaggtcccg gtc
//
LOCUS       DASUPW010000201         2175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1850502, whole genome shotgun sequence.
ACCESSION   DASUPW010000201 DASUPW010000000
VERSION     DASUPW010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1850502"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VFY44_04650"
     CDS             complement(<1..307)
                     /locus_tag="VFY44_04650"
                     /inference="COORDINATES: protein motif:HMM:NF042761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HEX5909759.1"
                     /translation="MTRAAIMAAAFGAAFGFLLSWGTLTDPDTIRAMLELKDAYVFLM
                     MGSAVGVGFAGVRLLTRRPTRALVTGEPVVARTDRIERRHLAGSAMFGLGWAIADTCP
                     "
     gene            complement(304..786)
                     /locus_tag="VFY44_04655"
     CDS             complement(304..786)
                     /locus_tag="VFY44_04655"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HEX5909760.1"
                     /translation="MAHRMEFLADRPPWFVLGPCLGLLIVGLLATLNVRMGVLGGWSD
                     VLDRANGRADTVSWKGWFFLGVIAGGLVFHLLAGQTTTGDGSGYGWLSREISSPVLVG
                     AVLVGAGALIGYGAKVAGGCTSGNGLGGTSFGSPAGLVSTATFMGTAVAATLLMGVVL
                     "
     gene            complement(1028..1666)
                     /locus_tag="VFY44_04660"
     CDS             complement(1028..1666)
                     /locus_tag="VFY44_04660"
                     /inference="COORDINATES: protein motif:HMM:NF015423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEX5909761.1"
                     /translation="MDEGFDATLLLWLNLVGTFVFGLSGGLAAVRARLDAFGVVVLAG
                     VVGLAGGVIRDLLIGVPPATFRDWRYLAAVGAAGAVCFVAPRAVDRLSPGVLFLDAVG
                     LAVFCVTGASKAIDYGLGPVQATLLGAITGIGGGMLRDVLLREVPTVLRHELYAIPAL
                     AGAAVVAIASEAGSDSAAFALTGAAVCFALRMVGLRYGIGVPSPRHRLGDEP"
     gene            complement(1701..>2175)
                     /locus_tag="VFY44_04665"
     CDS             complement(1701..>2175)
                     /locus_tag="VFY44_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909762.1"
                     /translation="GTAAERGDASGPKPAAGGTNATDRRSATDRRGAQHPTARSDTPA
                     HGGGDQTGAPAAGHENGADGGKALGHTKEPAPATGNGQGNGNAYGLNKPNRGNGIANG
                     HGPPAGAPGLGQGQGNGNGLGNGNGLGNGLDKVKEKVRSVAAPIAGPEEPEGKTK"
BASE COUNT          307 a          862 c          742 g          264 t
ORIGIN      
        1 ccgggcaggt gtcggcgatg gcccacccca ggccgaacat cgcgctgccg gccaggtggc
       61 ggcgctcgat ccggtcggtc cgggccacca ccggctcacc ggtgaccagg gcacgcgtcg
      121 gccgacgggt caggagcctg accccggcga agccgacgcc gaccgccgag cccatcatca
      181 ggaacacgta ggcgtccttc agctcgagca tcgcccggat cgtgtcggga tcggtcagcg
      241 tgccccagct gagcaggaag ccgaacgccg ccccgaacgc cgcggccatg atcgccgcgc
      301 gcgtcacagc accaccccca tcagcagcgt ggcggcgacg gccgtgccca tgaacgtggc
      361 cgtggacacc agccccgccg gggagccgaa cgaggtcccg cccaggccgt tgccggaggt
      421 gcagccgccc gcgaccttgg cgccgtagcc gatcagcgcc cccgccccca ccagcaccgc
      481 gccgaccagg acgggcgacg agatctcacg cgacagccat ccgtagccgc tgccgtcgcc
      541 ggtggtggtc tgtccggcca gcaggtggaa caccagcccg ccggcgatca ccccgaggaa
      601 gaaccagccc ttccaggaga cggtgtcggc ccggccgttg gcccggtcca gcacgtccga
      661 ccagcccccg agcacgccca tcctcacgtt cagcgtggcc agcagtccga cgatcagcag
      721 ccccaggcag gggccgagaa cgaaccacgg cggtcggtcg gccaggaact ccattcggtg
      781 cgccacctcc gggaaaaatc gattcttcac ctgacactag acaaccccgg tgggtcaagc
      841 gtccaacacg ggtgtttcac ccgcctgcgc tcggatagga cactggtacg cgagcggccc
      901 tgaaagaggg cgtgggggga cgggccccct tcgtctggtc gcgagcaccg agcgcggggc
      961 ccattccgag tgcgtttccc tcggtgggcc ccgagcgccc gttccgaccc ggccgcgcta
     1021 cggagaccta tggctcgtcg cccagacggt gacgcggcga cgggaccccg atcccgtacc
     1081 ggaggcccac catccgcagc gcaaagcaca ccgcggcccc cgtcagcgcg aacgccgcgc
     1141 tgtcgctccc ggcctcggac gcgatcgcga cgacggccgc gccggccagc gccgggatcg
     1201 cgtagagctc gtggcgcagc accgtcggca cctcgcgcag caggacgtcc cgcaacatgc
     1261 cgcccccgat gccggtgatg gcgccgagca gcgtcgcctg cacgggaccg aggccgtagt
     1321 cgatcgcctt gctcgccccc gtgacgcaga agacggcgag cccgacggcg tccaggaaca
     1381 gcacgcccgg gctgagccgg tccacggccc tcggggcgac gaagcacacc gcgcccgccg
     1441 ccccgacggc cgccagataa cgccagtcgc ggaaggtcgc cggcggcacg ccgatcagca
     1501 ggtccctgat cacgccgccc gcaaggccca ccacgccggc cagcacgacc accccgaacg
     1561 cgtccagcct ggcccggacg gcggccagcc cgccgctcag cccgaagacg aacgtcccga
     1621 ccaggttcag ccacagcagc agcgtcgcgt cgaagccctc gtccacggcg cgaggatacg
     1681 ggcgcggcgg cgccgggtcc ctacttcgtc ttgccctccg gctcctcggg cccggcgatg
     1741 ggcgcggcga ccgacctgac cttctccttg accttgtcga ggccgttgcc caacccgttg
     1801 ccgttgccca gcccgttgcc gttgccctgg ccctgcccca ggcccggagc gccggcgggc
     1861 gggccgtggc cgttggcgat gccgttgccg cggttgggct tgttcagccc gtaggcgttg
     1921 ccgttgccct ggccgttgcc cgtggccggc gccggctcct tcgtgtgccc gagcgccttg
     1981 ccgccgtcgg cgccgttctc gtggccggcg gcgggcgcgc cggtctggtc gcctccgccg
     2041 tgggcgggag tatccgaccg ggccgtcgga tgctgcgcgc cgcggcggtc cgtggcgctc
     2101 ctgcgatcgg tcgcgttcgt gccgcccgca gccggcttcg ggccggaggc gtcaccccgc
     2161 tcggccgcgg tcccg
//
LOCUS       DASUPW010000202         4954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_327513, whole genome shotgun sequence.
ACCESSION   DASUPW010000202 DASUPW010000000
VERSION     DASUPW010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327513"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VFY44_04670"
     CDS             <1..504
                     /locus_tag="VFY44_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909763.1"
                     /translation="ARLGRNRTKQRKRLEGTLLSDRKGIDRYCLKKGGGALRVGYPTS
                     RLASKLSKKTRKRIKSKAVYMGTTSKAFSISKLKVGSRTKTLRKRLKGEKRYVVGKNV
                     WYVAKGKKARVLFRARKSKVLELALADRGLTATKTGTVRLLRAWDKRGKTKAKAKKKS
                     KAKSKKK"
     gene            complement(598..1248)
                     /locus_tag="VFY44_04675"
     CDS             complement(598..1248)
                     /locus_tag="VFY44_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909764.1"
                     /translation="MPALDPPSARSLWVPTPARLVRLMAGLWLFGTGEGLLVHSDLGN
                     SPWTVLAEGVSVHSPLSIGAATILISFLVLLVWIPLRQRPGLGTIANAIIVGVGVDVT
                     LAYMPETSGVVPWLEIVAGIALVGLGSGLYINAALGPGPRDGLMTGIHGRTGWPIAPV
                     RAGIELSALTAGVVLGGTAGVGTLAFALGVGPAVALGLRLLPEGARPSSPAPAGSA"
     gene            1336..3588
                     /gene="pnp"
                     /locus_tag="VFY44_04680"
     CDS             1336..3588
                     /gene="pnp"
                     /locus_tag="VFY44_04680"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX5909765.1"
                     /translation="MSIDVTRVSVEIGGTEISFETGKLAKQASGSVVVQAGDTMVLGT
                     ATAGNLRDVDFLPLTVDVEERMYAAGKIPGSFFKREGRAGEKGTLTARMIDRPIRPLF
                     PKGWRRETQLVSIPMSIDHVHPYDILAMNGASAALMVSDIPFPQPVGAVRIGQDEEGN
                     FVVNPDEEDNLDASLDLVVSGTEEAILMVEAGAKEISEAAILDALDIAHGEIKKLCAA
                     QRELAEKAGKPKAEVEVPQVDESVFAQVKAEFETALTTATSVEDKLKRQDETKAVEEN
                     ALKSLSGDESAEDYKEKRAAVQMAFDKLEKDLIRKRIAIDKVRPDGRAADQVRDIWIE
                     VGVAPKTHGSTVFTRGQTQAFSVATLGTTRDEMRLDTLGLETSRRYWHHYNFPPFSVG
                     EAGFMRGPKRRDIGHGALAERALVPVIPSAEEFPYSIRVVSDIFESNGSSSMASVCGS
                     SLSLMDAGVQIKAPVAGIAMGLIKEGDDYIVLSDIAGVEDHLGDMDFKVAGTEKGITA
                     LQMDIKITGVTFDILSDALAQAKVGRTHILGKMAEAIEGPRPELSKNAPSIGQIQIDT
                     DKIGMIIGKGGETIRALSEEFEAQIDIDDDGTIRVFAINGDQGDALVERIESMTKEIE
                     VGDEFDGKVVKTTTFGAFIELSKGNDGLLHISNVKPGERVDAVEDVLNRGDEIAVRVV
                     EVDKERGRVGLRLADDPDIAGKSAEELASVGTGGGGGGGGGRDRGGRGGRDRGGRGGN
                     GGGGHSRDRR"
     gene            3630..4868
                     /locus_tag="VFY44_04685"
     CDS             3630..4868
                     /locus_tag="VFY44_04685"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5909766.1"
                     /translation="MVSERVDSVRSTALGIWMRTGSRDEAPEQAGLSHFLEHLLFKGT
                     DRFTSLEIDETFDAMGAEVNAGTSKETTTVYSRFLDRHLDRAFDVMSDMVLRPTYPDI
                     DSERQVVIEEIAMYEDEPSDKVHDVLADAVFGDHPLGRPVIGRAEVIAGVPVPDIAAY
                     RDEHYVSSNLVVAGAGSIDHARLVELVQGTLDDRVGAPAPGLVNGAAIPPDPRLCFHA
                     KETEQYHLALGAPGLARGDERRFALRVLDTILGGSSSSRLFQEVREKRGLAYAVYSFA
                     SQFIDSGQVGLYVGTRPDNVDEAMQVIGDELRRVAADGVTEAELERARENVKGRTTLA
                     MESTLARMNRLGSSLLTGVPLLSLDEVLARVDAVTLEDVQELAGELFDPARMSAAGVG
                     GSEDVFRRGVGKVNPELAAA"
BASE COUNT          886 a         1688 c         1752 g          628 t
ORIGIN      
        1 gcgcgcctgg ggcgcaaccg caccaagcag cgcaagaggc tcgagggcac gctgttgtcc
       61 gaccgcaagg gcatcgaccg ctactgcctg aagaagggcg ggggcgcgct gcgggtgggc
      121 tacccgacca gccggctggc ctcgaagctg tccaagaaga cccgcaagcg catcaagagc
      181 aaggccgtct acatgggcac cacgtccaag gccttctcga tctccaagct caaggtgggc
      241 tcgaggacca agacgctgcg caagcggctc aagggcgaga agcgctacgt ggtcggcaag
      301 aacgtctggt acgtggccaa gggcaagaag gcccgcgtcc tgttccgggc gcgcaagagc
      361 aaggtcctgg agctggccct ggccgaccgg ggcctgaccg ccaccaagac cggcacggtc
      421 cgcctgctgc gcgcgtggga caagcgcggc aagacgaagg cgaaggccaa gaagaagagc
      481 aaggcgaagt cgaagaagaa gtaggcccgt cccggcaaac ggttcatacc tttgggcggc
      541 cagagcgtct caaaggtatg aaccgcgatc tggggccgct ggctacccag ccgccgttca
      601 ggccgagccc gccggggcgg gtgacgaagg acgcgcaccc tccggcagca acctgagccc
      661 cagcgccacc gccggcccca cgcccagggc gaaggccagc gttcccacgc ccgcggtgcc
      721 gcccagcacc acgccggccg tgagcgccga gagctcgatc ccggcgcgca cgggcgcgat
      781 cggccacccg gtccggccgt ggatcccggt catcagcccg tcgcggggcc cggggccgag
      841 cgcggcgttg atgtacagcc cgctgcccag gcccaccagc gcgatcccgg ccacgatctc
      901 cagccagggc accacgcccg acgtctcggg catgtaggcc agggtcacgt cgacgcccac
      961 gccgacgatg atcgcgttgg cgatcgtgcc cagccccggc cgctggcgca gcgggatcca
     1021 gaccaggagc acgaggaacg agatcaggat cgtcgccgcc ccgatggaca gcggcgagtg
     1081 caccgacacg ccctcggcca ggaccgtcca cggcgagttg cccaggtcgg agtgcaccag
     1141 caggccctcg ccggtgccga acagccacag gccggccatc agccgcacca gccgcgcggg
     1201 tgtcggaacc cacagcgagc gggcagaagg agggtcgaga gcaggcaaga ccactgctag
     1261 cctgccggat tgactcctaa acgagaagtc accagagaga gagccgccaa ccggaggcgg
     1321 caccgaaagg aacatatgag tattgacgta acccgtgtct cagttgagat cggcggtacc
     1381 gagatcagct tcgagaccgg caagctcgcc aagcaggcga gcggctccgt ggtcgtgcag
     1441 gcgggggaca ccatggtcct cggcaccgcg accgcaggaa acctccgcga cgtcgacttc
     1501 ctcccgctga cggtggatgt cgaagagcgc atgtacgccg cgggcaagat ccccggcagc
     1561 ttcttcaagc gcgagggtcg cgccggcgag aagggcaccc tgaccgcccg catgatcgac
     1621 cgcccgatcc gcccgctctt ccccaaggga tggcggcgtg agacccagct ggtttcgatc
     1681 cccatgtcga tcgaccacgt gcacccctac gacatcctcg cgatgaacgg cgccagcgcc
     1741 gcgctgatgg tgtccgacat cccgttcccg cagcccgtcg gcgcggtgcg catcgggcag
     1801 gacgaggaag gcaacttcgt cgtcaacccc gacgaggagg acaacctcga cgcgtccctg
     1861 gacctcgtgg tctcgggcac cgaagaggcc atcctgatgg tcgaggccgg cgccaaggag
     1921 atctccgagg ccgccatcct ggacgccctg gacatcgcgc acggcgagat caagaagctg
     1981 tgcgccgccc agcgcgagct ggccgagaag gccggcaagc ccaaggccga ggtcgaggtc
     2041 ccgcaggtcg acgagtccgt cttcgcccag gtgaaggccg agttcgagac cgcgctgacc
     2101 accgcgacct ccgtcgagga caagctcaag cgccaggacg agaccaaggc ggtcgaggag
     2161 aacgcgctga agtcgctctc cggcgacgag agcgccgagg actacaagga gaagcgcgcc
     2221 gcggtccaga tggcgttcga caagctcgag aaggacctga tccgcaagcg gatcgcgatc
     2281 gacaaggtgc ggcccgacgg ccgtgccgcc gaccaggtgc gcgacatctg gatcgaggtc
     2341 ggcgtggccc cgaagacgca cggctcgacc gtgttcaccc gcggtcagac gcaggccttc
     2401 tccgtggcca ccctgggcac cacgcgcgac gagatgcgcc tggacaccct cggcctggag
     2461 accagccggc gctactggca ccactacaac ttcccgccct tctcggtcgg ggaggcgggc
     2521 ttcatgcgcg gtcccaagcg gcgtgacatc ggtcacggcg ccctggccga gcgtgcgctg
     2581 gtcccggtga tcccgtcggc cgaggagttc ccgtactcca tccgcgtggt ctcggacatc
     2641 ttcgagtcca acggctcgtc gtccatggcc tcggtctgcg gcagctcgct gtcgctgatg
     2701 gacgccggtg tgcagatcaa ggctccggtg gcgggcatcg ccatgggcct gatcaaggag
     2761 ggcgacgact acatcgtgct ctccgacatc gcgggcgtcg aggaccatct gggcgacatg
     2821 gacttcaagg tcgccggcac cgagaagggc atcaccgccc tccagatgga catcaagatc
     2881 acgggcgtga cgttcgacat cctgagcgac gcgctcgccc aggccaaggt cggccgcacg
     2941 cacatcctgg gcaagatggc cgaggccatc gagggcccgc gcccggagct gtccaagaac
     3001 gctccgagca tcggccagat ccagatcgac accgacaaga tcggcatgat catcggcaag
     3061 ggcggggaga ccatccgcgc gctgtccgag gagttcgagg cccagatcga catcgacgac
     3121 gacggcacca tccgcgtctt cgcgatcaac ggggaccagg gcgacgccct ggtcgagcgg
     3181 atcgagtcga tgaccaagga gatcgaggtc ggcgacgagt tcgacggcaa ggtggtcaag
     3241 accaccacgt tcggcgcctt catcgagctc tccaagggca acgacggcct gctgcacatc
     3301 agcaacgtca agcccggtga gcgcgtcgac gcggtcgagg acgtcctgaa ccgcggcgac
     3361 gagatcgccg tgcgcgtggt cgaggtcgac aaggagcgag gccgcgtcgg cctgcgcctg
     3421 gccgacgatc cggacatcgc cggcaagagc gccgaggagc tggcttcggt cggcaccggt
     3481 ggcggcggcg gaggcggcgg aggccgtgac cgcggtggac gtggcgggcg cgaccgtggc
     3541 ggtcgcggag gcaacggcgg cggcggacac agccgcgacc ggcgctgaac gacgcagccg
     3601 agataacgac gctcgattcg ggcgtccggg tggtcagcga acgggtcgat tccgttcgct
     3661 cgaccgccct cggcatctgg atgcggacgg gaagtcgcga cgaggccccc gagcaggcgg
     3721 gcctgtcgca cttcctggag catctgctct tcaagggcac cgaccgcttc acctcgctgg
     3781 agatcgacga gaccttcgac gccatgggcg ccgaggtcaa cgcgggcacc agcaaggaga
     3841 cgaccacggt ctactcacgg ttcctggacc gccacctgga ccgcgcgttc gacgtgatgt
     3901 cggacatggt cctgcgcccg acgtacccgg acatcgactc cgagcgccag gtggtgatcg
     3961 aggagatcgc catgtacgag gacgagccct cggacaaggt gcacgacgtg ctggccgacg
     4021 ccgtgttcgg cgaccacccg ctgggacgcc cggtgatcgg cagggccgag gtcatcgccg
     4081 gtgtgccggt gcccgacatc gccgcctacc gcgacgagca ctacgtctcg tcgaacctgg
     4141 tcgtggccgg cgccggcagc atcgatcacg cgcgcctggt ggagctggtg cagggcacgc
     4201 tggacgaccg cgtgggcgca ccggcgcccg ggctggtcaa cggcgcggcc atcccgcccg
     4261 acccacggct gtgcttccat gccaaggaga ccgagcagta tcacctggcg ctgggcgccc
     4321 cgggcctggc ccgcggtgac gagcgccggt tcgcgctgcg ggtgctggac accatcctgg
     4381 gcggatcgag ctcatcgcgg ctgttccagg aggtgcgcga gaagcgcggc ctggcctacg
     4441 cggtctactc cttcgccagc cagttcatcg actccggcca ggtcggcctg tacgtgggca
     4501 cgcgtcccga caacgtggac gaggccatgc aggtgatcgg cgacgagctg cgccgcgtgg
     4561 ccgccgacgg cgtgaccgag gccgagctgg agcgcgcgcg ggagaacgtg aagggccgca
     4621 ccacgctggc catggagtcc acgctggcgc gcatgaaccg cctgggaagc tcgctgctga
     4681 ccggcgtgcc gctgctgagc ctggacgagg tcctggcccg cgtggacgcg gtgacgctgg
     4741 aggacgtcca ggagttggcc ggagagctgt tcgacccggc ccggatgtcc gcggccgggg
     4801 tggggggcag cgaggacgtc ttccgccgcg gcgtcggcaa ggtgaacccg gagctcgcgg
     4861 cggcgtgatc cgcgtcgcgg tgtccggcgc cgccgggcgc atgggggcca cggtctgcgg
     4921 cgccgtcgag ggcgccgagg acatggagct gacg
//
LOCUS       DASUPW010000203         4150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_49317, whole genome shotgun sequence.
ACCESSION   DASUPW010000203 DASUPW010000000
VERSION     DASUPW010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49317"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VFY44_04690"
     CDS             complement(<1..243)
                     /locus_tag="VFY44_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909767.1"
                     /translation="MGRRRWTIALAAAAAVAAAPSAQAAKPKPSPVRVQVLSTTQNAV
                     ASSKRVRVRVSSARRGFVRLGGRVVTTPGGKRRIVRL"
     gene            386..982
                     /locus_tag="VFY44_04695"
     CDS             386..982
                     /locus_tag="VFY44_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909768.1"
                     /translation="MKRIVPSPAMAVAFVALLVAFTGSSFAAPAREAASKLVSGTKIT
                     KSSITSKQIKNGSLLAADFKKGQLPRGATGARGAQGPQGAQGAQGPQGQQGPGAPVAY
                     AEVGQSGALGKRSGAVSSVQENGGGSPGQYEVVFNRNISNCVYVASHVSDLASPAAGG
                     IATTGPLSAQDNQNAVSVSTRNSAGALAFAPFTIAVYC"
     gene            complement(1001..>4150)
                     /locus_tag="VFY44_04700"
     CDS             complement(1001..>4150)
                     /locus_tag="VFY44_04700"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909769.1"
                     /translation="VIPRNPLDVLAQQIVAVVATGDDWKAGELHELVRRAYPYSDLSR
                     ELLDSVLDMLDGRYPSDEFSELRPRIVWDRVEDTIRARKGARQLAVTNAGTIPDRGLY
                     GVHLPDGRRVGELDEEMVYEARPGQTFMLGASTWRIEEITRDRVIVTPAPGAPGALPY
                     WKGDGVGRPAELGRAIGEFARTAVDREPAELAADYDLDDRAASNLVTYLREQREATRV
                     VPSDQTIVVERFRDEIGDWRLCVLSPFGGRVHAAWGLALSARIRDRFGLESDAIWSDD
                     GIIVHLPDADEPPGAELVLVEPDEVEELVVGELSASALYGARFRENAARALLLPRAYP
                     GKRTPLWQQRLKAQSLLEVAKKYGQFPIVLETYRECLRDVLDLPGLEELLRGLHTREL
                     SLVEAETQRASPFASSLLFDYVATYMYEGDTPNAERRAAALSLDRDLLRELLGQEELR
                     DLIDPAALAEVEDDLQRRSERTRAANADSLHDVLRSVGDLTRQEMDERVLPDVSVDAM
                     LEVLSGDRRAVEMRVGGEQRWIASEDAGLYRDAFGAVPPGGLPAAFLDETPDPFQRLV
                     RRFARTHGPFTTRELHDRFGVDPTPVLQALESDGGLVRGELRPGGSEREWCDTDVLRR
                     LRRASLAALRKEVEPAEQQALARFLPSWQGVDTSPPGGAGVDRLRELLVPLQGVALAP
                     SVWEQDVLPRRAGAYSAGWLDQLCASGEVVWVGAGTLGRSTGKVTLYFREDAPLIGPP
                     NIKGDPPAGEVHDAIRERLRAGASFWTDLLADVAREPAELQEALWDLVWAGEVTNDAF
                     APLRAPRLSLAKQQSGTARRFSRRRRPGTPQVQGRWSLTDRLFAGAPEHSTRMRAISE
                     LMLERYGIVTRETVLAEGIPGGFSSLYGELANLETLGTARRGYFVEGLGGAQFALPAA
                     IERLRGLRSDEPAGALVLAATDPANPYGAQLPWPKRENEPSRPRRVRGAHLVTLDAEP
                     SLYVEKGGKGLLALRDPAEGDWLPAAFEALAEHVRRGRLKRLAVERFDGQPVVGSPYE
                     QLLIDAGFRLGPRRLTLSA"
BASE COUNT          539 a         1570 c         1536 g          505 t
ORIGIN      
        1 gagccggacg atccggcgct tgccgcccgg tgtggtcacc acgcggcccc ccaggcgcac
       61 gaagccgcgc ctggccgacg acacccgcac gcggacgcgc ttggaggagg ccaccgcgtt
      121 ctgggtcgtg ctcagcacct ggacgcgcac gggggagggc ttgggcttcg cggcctgcgc
      181 cgagggggct gccgccacgg ccgccgccgc ggccagcgcg atcgtccagc gccgcctccc
      241 catggcccga tcttgccacc cccaaggccg cccgtgcgcc ggtccgccca atactggggg
      301 ccgggtttag ggggcctcct gatggcaggg cggccgggcc gggcgagggt ggcgccagtc
      361 aaccacgacc tggaggtcaa tccgcatgaa gcgcatcgtt ccctcacccg ccatggccgt
      421 cgccttcgtg gcactgctgg tggcgttcac cggctcgtcg ttcgccgccc cggcgcgcga
      481 ggccgcgtcc aagctggtca gcggcaccaa gatcacgaag agctcgatca ccagcaagca
      541 gatcaagaac ggatccctgc tggccgccga cttcaagaag ggccagctgc cccgcggcgc
      601 gaccggcgct cgcggcgccc agggcccgca gggcgcccag ggcgctcagg gtccccaggg
      661 ccagcagggg cccggcgccc cggtggccta cgcggaggtc ggccagagcg gcgcgctggg
      721 caagcgctcc ggtgccgtct cgtccgtcca ggagaacggc ggcggtagcc ccgggcagta
      781 cgaggtggtc ttcaaccgca acatctcgaa ctgcgtctac gtggcctccc acgtcagcga
      841 cctggcctcg ccggctgccg gcgggatcgc caccaccggg ccgctgagcg cccaggacaa
      901 ccagaacgcc gtctcggtga gcacgcgcaa cagcgccggt gcgctggcct tcgcgccgtt
      961 caccatcgcc gtctactgct gaggggctcg cccgcggcgg tcaggcgctc agcgtcagcc
     1021 gccgcgggcc cagccggaag cccgcgtcga tgagcagctg ctcgtacggc gagccgacca
     1081 cgggctgccc gtcgaagcgc tcgaccgcca gccgcttgag gcggccgcgg cgcacgtgct
     1141 cggccagcgc ctcgaacgcg gcggggagcc agtcgccctc ggccgggtcg cgcagcgcca
     1201 gcagcccctt gccgcccttc tccacgtaca gggacggctc ggcgtccagg gtcacgaggt
     1261 gggctccgcg gacgcggcgg gggcgggatg gctcgttctc gcgcttgggc cacggcagct
     1321 gggcgccgta ggggttggcg gggtccgtgg cggccagcac cagcgcgccc gccggctcgt
     1381 cggaacgcag gccgcgcagc cgctcgatgg cggcgggcag cgcgaactgg gcgccgccga
     1441 ggccctcgac gaagtaaccg cggcgcgcgg tgcccagcgt ctccaggttg gccagctcgc
     1501 cgtacagcga cgagaacccg ccggggatgc cctcggccag caccgtctcg cgggtgacga
     1561 tgccgtagcg ctccagcatc agctcggaga tggcgcgcat gcgggtggag tgctccggcg
     1621 cgccggcgaa cagccggtcg gtcagcgacc agcggccctg cacctgcggg gtgcccgggc
     1681 ggcgccggcg cgagaagcgg cgggccgttc ccgactgctg cttggccagc gacagccgcg
     1741 gggcgcgcag gggcgcgaac gcgtcgttgg tgacctcgcc cgcccacacc aggtcccaca
     1801 gcgcctcctg gagctccgcc ggttcccggg ccacgtcggc cagcaggtcg gtccagaacg
     1861 acgccccggc gcgcaggcgc tcgcggatcg cgtcgtggac ctcgcccgcg ggcgggtcgc
     1921 ccttgatgtt cggcgggccg atcagcgggg cgtcctcacg gaagtagagg gtcaccttgc
     1981 ccgtgctgcg gcccagggtc ccggcgccca cccagacgac ctcgcccgac gcgcacagct
     2041 ggtccagcca cccggcggag taggcgcctg cgcgtcgggg cagcacgtcc tgctcccaga
     2101 ccgacggcgc cagggcgacg ccctgcagcg ggaccagcag ctcgcgcagc cggtcgaccc
     2161 cggcgccacc ggggggcgag gtgtccacgc cctgccacga gggcaggaag cgcgccagcg
     2221 cctgctgctc ggccggctcg acctccttgc gcagcgcggc cagcgaggcc cgccgcagcc
     2281 ggcgcagcac gtcggtgtcg caccactcgc gctcgctgcc gccggggcgc agctcgccgc
     2341 ggaccaggcc accgtcggac tccagcgcct ggagcaccgg tgtggggtcc acgccgaacc
     2401 ggtcgtgcag ctcgcgggtg gtgaacgggc cgtgcgtgcg ggcgaagcgc cgcaccaggc
     2461 gctggaacgg gtcgggcgtc tcgtccagga aggcggccgg cagcccgccg gggggcacgg
     2521 cgccgaaggc gtcgcggtac aggccggcgt cctcggaggc gatccagcgc tgctcgccgc
     2581 ccacccgcat ctcgaccgcg cgccggtcgc ccgacaggac ctcaagcatg gcgtcgaccg
     2641 acacgtcggg cagcacgcgc tcgtccatct cctggcgggt caggtcgccg acggagcgca
     2701 gcacgtcgtg cagcgagtcg gcgttggcgg cccgcgtccg ctcgctgcgc cgctgcaggt
     2761 cgtcctccac ctcggccagc gcggccgggt cgatcaggtc gcgcagctcc tcctggccga
     2821 gcagctcgcg cagcaggtcg cggtccagcg acagcgcggc ggcgcggcgc tccgcgttgg
     2881 gcgtgtcgcc ctcgtacatg tacgtggcca cgtagtcgaa cagcagcgac gaggcgaacg
     2941 gagacgcgcg ctgggtctcg gcctcgacca ggctgagctc gcgggtgtgc agcccacgca
     3001 gcagctcctc caggccgggc aggtcgagca cgtcgcgcag gcactcgcgg taggtctcca
     3061 gcacgatcgg gaactggccg tacttcttgg ccacctccag gagcgactgc gccttcagcc
     3121 gctgctgcca cagcggcgtg cgcttgcccg ggtaggcgcg cgggagcagc agggcgcggg
     3181 cggcgttctc gcggaagcgg gcgccgtaca gggcggaggc cgacagctcg ccgaccacca
     3241 gctcctcgac ctcgtccggc tcgaccagaa ccagctcggc cccgggcggc tcgtccgcgt
     3301 cgggcaggtg gacgatgatg ccgtcgtcgg accagatggc gtcggactcg agcccgaagc
     3361 ggtcgcggat ccgggcgctc agggccaggc cccacgccgc gtgcacgcgg ccgccgaacg
     3421 gggacagcac acacagccgc cagtcgccga tctcgtcgcg gaagcgctcg accacgatcg
     3481 tctggtcgct gggcaccacg cgcgtggcct cccgctgctc gcgcaggtag gtgaccaggt
     3541 tcgaggcggc gcggtcgtcc aggtcgtagt cggcggccag ctcggcgggc tcgcggtcga
     3601 ccgccgttcg ggcgaactcg ccgatggccc tgcccagctc ggccgggcgg ccgacgccgt
     3661 cgcccttcca gtagggcagc gccccgggcg caccgggggc gggggtcacg atcacccggt
     3721 cgcgggtgat ctcctcgatc cgccaggtcg aggcgcccag catgaacgtc tgcccggggc
     3781 gggcctcgta gaccatctcc tcgtccagct cgccgacgcg gcggccgtcg ggcaggtgca
     3841 cgccgtacag gccgcggtcg gggatcgtgc cggcgttggt caccgccagc tggcgcgcgc
     3901 ccttgcgcgc gcggatggtg tcctccaccc ggtcccacac gattcgcggc cgcagctcgg
     3961 agaactcgtc ggacgggtag cggccgtcca gcatgtccag cacgctgtcc agcagctcgc
     4021 ggctcaggtc ggagtagggg taggcgcgcc gcaccagctc gtgcagctcg cccgccttcc
     4081 agtcgtcgcc cgtggccacc accgccacga tctgctgggc cagcacgtcc agcgggttgc
     4141 gcgggatcac
//
LOCUS       DASUPW010000204        10251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1157996, whole genome shotgun sequence.
ACCESSION   DASUPW010000204 DASUPW010000000
VERSION     DASUPW010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 10251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10251
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157996"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /gene="groEL"
                     /locus_tag="VFY44_04705"
     CDS             <1..1138
                     /gene="groEL"
                     /locus_tag="VFY44_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX5909770.1"
                     /translation="EKVGKDGVVNVEEGQTFGMDLEFTEGMQFDKGYISPYMVTDQDR
                     MEATLEDPYILIANQKIGSVRDVLPVLEQVIQSGKPILIIAEDVEGEALATLVVNKLR
                     GTFTGVAVKAPGFGDRRKRMLEDIAILTGAEVITEEMGLKLENTQLTQLGRARRIVVD
                     KDSTTIVDGSGDSDAIKGRINQIKNEVESTDSDFDREKLQERLAKLAGGVAVVKVGAA
                     TETELKEKKHRVEDALQATRAALEEGIVTGGGVALLRSVDAVKVDALEGDEQTGARII
                     LRALEEPARQLAENAGLEGSVVVNDVRKAKKGHGLNAETGEIVDLVAAGVIEPAMVTR
                     SALQNAASIAKNILTTEAIVAEIEDENGGGGMPGGGMPDMGGMM"
     gene            1275..1799
                     /locus_tag="VFY44_04710"
     CDS             1275..1799
                     /locus_tag="VFY44_04710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5909771.1"
                     /translation="MNDLRDPQQFDRAYNEHAPAMLASANRVLRDNAASEDVVHDVFM
                     ALWRKPDSFDPARGNLGSYLTMMARSRALDRWRTRVARESAVDRVKAQIRPESVVSED
                     AAEPVVRRDSSRRVLHALDKLPGDQREAVLLAFGKGLTAREIAKTAGVPLGTAKSRVR
                     LGLAKARTELTAAA"
     gene            1796..2662
                     /locus_tag="VFY44_04715"
     CDS             1796..2662
                     /locus_tag="VFY44_04715"
                     /inference="COORDINATES: protein motif:HMM:NF021577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DICT sensory domain-containing protein"
                     /protein_id="HEX5909772.1"
                     /translation="MSTEGLSIREVTSRTGVGAATLRMWEQRHGFPVPKRLPSGHRRY
                     SHLDVELIRQVMRDRESGLELKAAVDNAKRAASGERPVIEDDSIYSGLRRRRPDLKSY
                     LLTKATLIQVSHAIEDECASASQRGLLFGSFQRESFYRSAEPRWRDLAATAECAFVLA
                     DFGEVSAPDDGPTEIPIDGSEPVGREWSLVYDAPAFGAVLSGWERPGQDDVADMDRRF
                     EALWSVEPELVRDASTVAYGIVERVAPETVEGIEELLARPVEPQQPSLTQLTALTNRM
                     VAYVGGAGFQTA"
     gene            complement(2659..3543)
                     /locus_tag="VFY44_04720"
     CDS             complement(2659..3543)
                     /locus_tag="VFY44_04720"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX5909773.1"
                     /translation="MILLTGATGTVGRAVLRRLIDAGSEPVRCLVRDPRRLGPDRVRV
                     QLTLGDLADPGAFRQALRGVQTVVHLGASIRDQPSGSLEALNGLATVRLVRAAERAGA
                     SRFLFFSALGASAQSDARFMRSKAKAAGAVAASALQSTVFAPSIVYTPGDPWLSLLER
                     LALLPALPVSGDGRAVYQPIWAEDVADCVVAALDRPGSDGHREFDLAGPQSLSYDDIV
                     RAALLPLGRSRPLVHVPLPVVKAVLNGGERVLGPAAPATWEEAKLMEESMTTSRGTAD
                     AEALGVNPLRMSAVLGSA"
     gene            3596..3871
                     /locus_tag="VFY44_04725"
     CDS             3596..3871
                     /locus_tag="VFY44_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX5909774.1"
                     /translation="MAYVIAEPCIGQKDNSCVEVCPVDCIHPTPDEPDYDNVEQLFID
                     PEECIDCDACVEACPVDACFAEDQLPDEWSKFTEVNAAYFKDGAGAP"
     gene            complement(3868..5022)
                     /locus_tag="VFY44_04730"
     CDS             complement(3868..5022)
                     /locus_tag="VFY44_04730"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEX5909775.1"
                     /translation="MPTEQQITDALRKVIDPELRKDIVELGMVRSIEPGAGGSVHIRV
                     SLTTAGCPIRSHFITAVQENVGALEGIESVSVDFDVLSDQEKQTLQQRLGRQGGLPQG
                     ALARVRNVICVGSGKGGVGKSTVTANLAAALKAEGKSAACMDADVWGYSIPRMLGVHG
                     RPKVTADRKIVPLEGAGGVRAISIEFFLDREDQAITWRGPMLHKAIRQFLEDVDWGEL
                     DYLLIDLPPGTGDVSMTLAQLLPQSKFVIVTTPQPAAQKVAKRAADTAQRYDLEIAGV
                     VENMSGFVTPDGDRVAIFGEGGGQELADELDVPLLGKIPLEEALRVGADEGRPLVLDD
                     PDAPSAQAIRHTARGLIAATPQVDPVFQERSGPALEGVPEITGTALPMAN"
     gene            complement(5012..5548)
                     /locus_tag="VFY44_04735"
     CDS             complement(5012..5548)
                     /locus_tag="VFY44_04735"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5909776.1"
                     /translation="MPGDVHDPVSRASYSFTRNGDDLYVETWMEPGGGLPPHWHPRQE
                     EVWFCIDGRVEFRLGKEKRVIGPEDGEMLVRRDTVHGLKAVEDRTVHLGCRVSPALDL
                     EAFLTESAAAANQGLFMKGGIPKGLKGTRWAADFLDRYGDETVMVFPPQFVQKATKAL
                     FGGRGKRAPSATMESDAH"
     gene            5598..6200
                     /locus_tag="VFY44_04740"
     CDS             5598..6200
                     /locus_tag="VFY44_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5909777.1"
                     /translation="MASTATPRLARSERRDLVLRAGGAEFGRRGYAAARIEDIAAAAG
                     VTKPIVYRHFESKKGLYLALLRKHETDLPTFLEGAGEGTTPILELWLDYVRANSHAWL
                     MLFRDHSGDDEIRRVREEVSVTARKVLAAFVAARRPDLDPAEVEPIAEMLTSGLAGLA
                     LWWIDRPETPKAVVLAAAASITEPVLTTKKTDGATKDAAG"
     gene            complement(6336..6992)
                     /locus_tag="VFY44_04745"
     CDS             complement(6336..6992)
                     /locus_tag="VFY44_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909778.1"
                     /translation="MAEQTATQRKAAAKKAAATRKRNAATSGAKSTRSAAKGTATTAQ
                     RNTRNTRRSTSSAAKRTATTAQRTAKTGAESGILSVEALAVNAERAVLIPVGAALTAR
                     DNVVEAVAPYTSRDTAEKEITKLQKQVTTDLKKYERRGNTARNRVRREAKRTRTRVER
                     ELRQRRNQATRLVKRNRREAEKQVKAVRRDVEAQVKTTRSEVETQINKVQDQVTATLS
                     "
     gene            complement(7126..8160)
                     /locus_tag="VFY44_04750"
     CDS             complement(7126..8160)
                     /locus_tag="VFY44_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX5909779.1"
                     /translation="MIDWGVARQIARFASRPGQMPDLGVDLPALARELEAPVGAHTGL
                     APDGPVPPVEVVGRAEWAEANLATLSDLLDPVAGRMEHRFDAAGPFAGALRAGTGVTM
                     AAEVGLVTGFMAQHVLGQYELSLLALHPRPRLLMVAPNLDQAAAKLGADRESFLRWVT
                     IHELVHAFQFGGVPWLREHLGGLLGDYLETVDVQISSGAAGGAPSLPNPGELVARFQE
                     GGLAALVQSPAQREIMERVQVAMAVVEGHAEHVMDALAPELVPHHEGLRDAMEQRRDD
                     RSAPQRILMRLLGMDMKMRQYRQGKAFCDAVAAEGGTALLNRVWESPASLPSAGELSR
                     PARWTARIAA"
     gene            complement(8157..9068)
                     /gene="ligD"
                     /locus_tag="VFY44_04755"
     CDS             complement(8157..9068)
                     /gene="ligD"
                     /locus_tag="VFY44_04755"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX5909780.1"
                     /translation="MAAKAVEVDVQGRKLRLSNLEKVLYPEAGFTKGQVIDYYTRAAP
                     VLLNHLRGRALTLKRYPNGVDDKYFYEKQRPSHAPEWVRSMPIQSGRRGDHRVIDYVL
                     CDDLPTMVWLANLADLELHPSLALAEDPDSPTVMAFDLDPGAPAALGECCEVALILRD
                     TLDHLGLECHPKTSGSKGMQVYVPLNTGAGYDDVKPFAHGVAKVLEQRHDLIVSVMKK
                     EVRKGKVFIDWSQNDRHKTTVGPYSLRARPRPTVSTPLSWDEVDHGAGGGAADLVFES
                     ADVLERIEEHGDLFAGVLETHQELPDL"
     gene            complement(9069..9968)
                     /locus_tag="VFY44_04760"
     CDS             complement(9069..9968)
                     /locus_tag="VFY44_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX5909781.1"
                     /translation="MARAIWSGAISFGLVNIPVKLYSAVQRKTVRFNQLDGADNTRIQ
                     QKRVNPDSGEEVPYDRLVKGYEISPDRYVIIEPAELDSLQPEKTRTIDIQDFVDLHEI
                     DPIYYDHPYYLAPGTGAEKAYKLLYDAMEASEKVGIARVVIRSKEQLVAIRPREGILA
                     METMLFSDEVVSPEDVADMPEGKKAKVSKKEVDMAQQLIESLTTEFDPSQYRDEYRDA
                     VIEMIERKAEGHEIEVSAPPDEEPDEVPDLMAALEASISGSKRQSKPKTQPKPKAKAK
                     SSSSNGSKSKAKPKSKAKSTAKK"
     gene            complement(9996..>10251)
                     /locus_tag="VFY44_04765"
     CDS             complement(9996..>10251)
                     /locus_tag="VFY44_04765"
                     /inference="COORDINATES: protein motif:HMM:NF016557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909782.1"
                     /translation="YAGKVGSGFTQATLASTVAALGPLATGESPFAGRQPPKGTQFVE
                     PELVAHVEFGEWTRTGTLRAPVFRGLREDMDAQEVVREEG"
BASE COUNT         1473 a         3652 c         3585 g         1541 t
ORIGIN      
        1 cgagaaggtc ggcaaggacg gcgtcgtgaa cgtcgaggag ggccagacgt tcggcatgga
       61 cctcgagttc accgagggca tgcagttcga caagggctac atctcgccct acatggtcac
      121 cgaccaggac cgcatggagg cgacgctcga ggatccgtac atcctcatcg ccaaccagaa
      181 gatcggctcc gtccgcgacg tgctgcccgt gctggagcag gtcatccagt cgggcaagcc
      241 gatcctgatc atcgccgagg acgtcgaggg cgaggccctg gcgaccctgg tcgtgaacaa
      301 gctccgcggc accttcaccg gtgttgcggt caaggccccg ggcttcggcg accgccgcaa
      361 gcgcatgctc gaggacatcg cgatcctcac gggcgccgag gtcatcaccg aggagatggg
      421 cctcaagctg gagaacaccc agctgaccca gctcggccgt gcccgccgca tcgtggtcga
      481 caaggactcg accaccatcg tggacggttc cggcgactcc gacgccatca agggccggat
      541 caaccagatc aagaacgagg tcgagtccac ggactccgac ttcgaccgtg agaagctgca
      601 ggagcgcctg gccaagctgg ccggcggtgt tgcggtggtc aaggtcggcg ctgccaccga
      661 gaccgagctc aaggagaaga agcaccgcgt cgaggacgcg ctgcaggcca cccgcgccgc
      721 cctcgaggag ggcatcgtga ccggtggcgg cgtggcgctg ctccgttcgg tcgacgcggt
      781 caaggtcgac gccctcgagg gcgacgagca gaccggcgcg cgcatcatcc tgcgtgctct
      841 ggaggagcct gcccgtcagc tcgccgagaa cgcggggctc gagggctccg tggtggtcaa
      901 cgacgtccgc aaggcgaaga agggccacgg cctgaacgcc gagacgggcg agatcgtgga
      961 cctggtcgcg gccggcgtga tcgagccggc catggtcacc cgctctgccc tgcagaacgc
     1021 ggcgtccatc gccaagaaca tcctcacgac cgaggccatc gtggccgaga tcgaggacga
     1081 gaacggcggc ggcggcatgc cgggcggcgg catgcccgac atgggcggca tgatgtagcc
     1141 agagcagcgc tctcgccacg agagcctgtc gcagcacacg aaggcccggc cattgcgccg
     1201 ggccttcgtc gttgcggagg agaaaatcct ctacactctg ttgagataat cctcaacaag
     1261 agcgaggaac cctcatgaac gacctccgtg acccccagca gttcgaccgc gcgtacaacg
     1321 aacatgcccc cgcgatgctc gcctccgcca accgcgtcct gcgcgacaac gcagcgtccg
     1381 aggacgtggt ccacgatgtg ttcatggccc tgtggcgcaa gcccgactcg ttcgaccccg
     1441 cgcgcgggaa cctggggagc tatctgacga tgatggcccg cagccgcgcg ctcgaccgct
     1501 ggcgcacccg cgtggcccgc gagtccgccg tcgaccgcgt caaggcccag atccggcccg
     1561 agtccgtggt gtccgaggac gccgccgagc cggtcgtgcg ccgcgacagc agccggcgcg
     1621 tgctccacgc cctggacaag ctgccgggcg accagcgcga ggcggtgctg ctggccttcg
     1681 gcaaggggct gaccgcgcgg gagatcgcca agacggccgg cgtgccgctg ggcaccgcca
     1741 agagccgtgt ccgcctgggg ctggccaagg cccgcacgga gctgaccgca gccgcgtgag
     1801 caccgagggg ctcagcatcc gtgaggtgac cagccgcacg ggtgtcgggg ccgccaccct
     1861 gcggatgtgg gagcagcgcc acggcttccc cgtccccaag cgcctgccca gcggccaccg
     1921 ccgctactcg cacctggacg tcgagctgat ccggcaggtc atgcgcgacc gggagtccgg
     1981 cctcgagctc aaggccgccg tcgacaacgc caagcgcgcc gcgagcggcg agcgcccggt
     2041 gatcgaggac gactcgatct actcggggct gcgccggcgc cggccggacc tgaagtccta
     2101 tctgctcacc aaggcgacgc tgatccaggt cagccacgcg atcgaggacg agtgtgcgtc
     2161 cgcgtcccag cgcggcctcc tgttcggcag cttccagcgc gagagcttct accgctcggc
     2221 cgagccccgc tggcgcgacc tggccgccac cgccgagtgc gcgttcgtgc tggccgactt
     2281 cggcgaggtc tccgcccccg acgacggccc caccgagata cccatcgacg gcagcgagcc
     2341 ggtcggccgc gagtggtcgc tggtatacga cgcgcccgcc ttcggagccg tgctcagtgg
     2401 ctgggagcga ccgggacagg acgacgtggc ggacatggac cgccgtttcg aggccctgtg
     2461 gagcgtggag cccgagctgg tgcgcgacgc gtccaccgtg gcctacggga tcgtcgagcg
     2521 ggtggccccg gagacggtcg agggcatcga ggaactgctg gcgcggccgg tcgagcccca
     2581 gcagcccagc ctgacccagc tgaccgcgct gaccaaccgg atggtcgcct acgtgggcgg
     2641 cgccgggttc cagacggcct aggccgagcc cagcaccgcg ctcatcctga gcgggttcac
     2701 gcccagcgcc tcggcgtcgg ccgtgccccg cgaggtcgtc atcgactcct ccatcagctt
     2761 ggcctcctcc caggtggccg gcgcggccgg ccccaggacc cgctcgcccc cgttcagcac
     2821 cgccttcacc accggcagcg gcacgtgcac cagcggccgc gaccggccca ggggcagcag
     2881 cgcggcgcgc acgatgtcgt cgtaggacag cgactgcggc ccggccaggt cgaactcgcg
     2941 gtggccgtcc gagcccggcc ggtccagggc ggcgaccacg cagtcggcca cgtcctcggc
     3001 ccagatcggc tggtacaccg cgcggccgtc cccggacacc ggcagcgccg gcagcagggc
     3061 caggcgctcc agcagcgaca gccaggggtc cccgggcgtg tagacgatcg acggcgcgaa
     3121 cacggtgctc tggagcgcgc tggcggccac cgcgcccgcg gccttggcct tgctgcgcat
     3181 gaaccgggcg tcggactggg cgctggcgcc cagggccgag aagaacagga accgcgatgc
     3241 gccggcgcgc tcggccgccc gcaccaggcg caccgtggcc agcccgttca gggcctccag
     3301 cgatccggac ggctggtcac ggatcgacgc gcccaggtgg accacggtct ggacgccgcg
     3361 cagcgcctgg cgaaacgcgc ccgggtcggc caggtcgccg agggtcagct ggacccgcac
     3421 acggtccggt cccaggcggc gcgggtcgcg caccaggcag cgcaccggct cggacccggc
     3481 gtcgatcaac cggcgcagta ccgcgcggcc gaccgtaccc gtcgctcccg tgagtagaat
     3541 cacgcgtcgc cgagactaac ggcgctggac ccccgatctc gcaaggaact ctcccatggc
     3601 ctacgtcatc gccgaaccgt gcatcggcca gaaggacaac tcgtgtgtcg aggtgtgccc
     3661 ggtggactgc atccacccca ccccggacga gcccgactac gacaacgtcg agcagctgtt
     3721 catcgacccc gaggaatgca tcgactgcga cgcgtgcgtc gaggcctgcc ccgtcgacgc
     3781 ctgcttcgcc gaggaccagc tgccggacga gtggtcgaag ttcaccgagg tgaacgccgc
     3841 ctacttcaag gacggagcgg gcgcgcccta gttggccatg ggaagcgcgg tgcccgtgat
     3901 ctcgggcacg ccttccagtg ccgggcccga gcgctcctgg aagaccgggt ccacctgtgg
     3961 cgtggcggcg atcaggccgc gcgccgtgtg gcggatcgcc tgcgcggacg gggcatccgg
     4021 gtcgtccagc accagcgggc ggccctcgtc ggcgcccacg cgtagggcct cctcgagggg
     4081 gatcttgccc agcagcggca cgtccagctc gtcggccagc tcctggccgc cgccctcgcc
     4141 gaagatggcc acgcggtcgc cgtccggggt cacgaagccg gacatgttct ccaccacgcc
     4201 ggcgatctcc aggtcgtagc gctgcgcggt gtcggccgcg cgcttggcga ccttctgcgc
     4261 cgcgggctgc ggcgtggtga cgatcacgaa cttggactgc ggcagcagct gcgccagcgt
     4321 catcgagacg tcgcccgtgc ccgggggcag gtcgatcagc aggtagtcca gctcgcccca
     4381 gtccacgtcc tccaggaact ggcggatggc cttgtgcagc atcggcccgc gccaggtgat
     4441 ggcctggtcc tcacgatcca ggaagaactc gatcgagatg gcgcggacgc cgccggcgcc
     4501 ctcgagcggc acgatcttgc ggtcggccgt gaccttgggc cggccgtgca cgcccagcat
     4561 gcgcgggatc gagtagcccc acacgtcggc gtccatgcag gcggcggact tgccctcggc
     4621 cttgagcgcg gcggccaggt tggcggtcac ggtggacttg ccgacgccgc ccttgcccga
     4681 gccgacgcag atgacgttcc tgacccgggc cagcgcgccc tgcgggagcc cgccctggcg
     4741 gcccaggcgc tgctgcaggg tctgcttctc ctggtcggag agcacgtcga agtcgacgct
     4801 gaccgactcg atgccctcga gcgcgcccac gttctcctgg acggcggtga tgaagtgcga
     4861 gcggatcggg cacccggccg tggtcaggga gacccggata tggacgctgc cgccggcgcc
     4921 gggctcgatc gagcggacca tgcccagctc gacgatgtcc ttgcgcagct cggggtcgat
     4981 gaccttcctg agggcgtcgg tgatctgctg ttcagtgggc atcgctttcc attgtcgcag
     5041 agggcgcgcg cttgcccctt cctccgaaca gggccttcgt ggccttctgc acgaactgcg
     5101 gcgggaacac catgacggtc tcgtcgccgt agcgatccag gaagtccgcg gcccaccgcg
     5161 tgcccttcag ccccttcggg atcccgccct tcatgaacag gccctggttg gcggcggcgg
     5221 cgctctcggt caggaaggcc tcgaggtcga gcgcgggtga cacgcggcac cccaggtgca
     5281 ccgtgcggtc ctcgaccgcc ttgagcccgt ggaccgtgtc ccggcggacc agcatctcgc
     5341 cgtcctcggg cccgatcacg cgcttctcct tgcccaggcg gaactccacc cggccgtcga
     5401 tgcagaacca cacctcctcc tggcgcgggt gccagtgggg cggcaggcca ccgccgggct
     5461 ccatccaggt ctccacgtac aggtcgtctc cgttgcgcgt gaacgagtac gaggcgcggg
     5521 agacagggtc gtgaacgtcg ccgggcatgc gggctcctct cggttctccg gtgttaccgc
     5581 ttggtaactt atcaccgatg gccagcaccg caacccccag gctcgcgcgc tccgagcggc
     5641 gcgacctggt gctccgggcc gggggcgccg agttcgggcg ccggggctac gcggcggccc
     5701 ggatcgagga catcgccgcc gccgccggcg tgaccaagcc catcgtctac cgccacttcg
     5761 agtccaagaa ggggctgtac ctggcgctgc tgcgcaagca cgagaccgac ctgcccacct
     5821 tcctggaggg ggccggcgag ggcaccaccc ccatcctcga gctgtggctg gactacgtcc
     5881 gggcgaactc gcacgcctgg ctcatgctgt tccgcgacca ctccggcgac gacgagatcc
     5941 gcagggtgcg ggaggaggtc agcgtgacgg cgcgcaaggt gctggccgcg ttcgtggccg
     6001 cgcgccgccc ggatctcgac ccggccgagg tcgagcccat cgccgagatg ctgaccagcg
     6061 gcctggccgg cctggccctg tggtggatcg atcgcccgga gacgccgaag gcggtcgtcc
     6121 tggccgcggc ggcgagcatc accgagcccg tcctgaccac gaaaaaaacc gacggcgcca
     6181 caaaggacgc cgccggctga gggaggagag atactgctac tgccgacgag atatgaggtc
     6241 ggcagcctta ccccggaccc ggtctctccg gccggccggg gggaagtgtt cgcgaccggg
     6301 ccccgcgtcc ttgcggggcc ggtcgctgat gacgcttacg acagagtcgc ggtcacctgg
     6361 tcctgcacct tgttgatctg cgtctcgacc tcggaccggg tcgtcttgac ctgggcctcg
     6421 acgtcgcgac ggacggcctt gacctgcttc tcggcctcgc ggcggttgcg cttgaccagg
     6481 cgggtggcct ggttgcggcg ctgacgcagc tcacgctcga cgcgggtgcg cgtgcgcttg
     6541 gcctcgcggc ggacacggtt ccgagcggtg ttgccacgac gctcgtactt cttgaggtcg
     6601 gtggtcacct gcttctggag cttggtgatc tccttctcgg cggtgtcgcg gctggtgtac
     6661 ggagccacgg cctcgaccac gttgtcacgc gcggtcaggg cggcaccgac ggggatcagc
     6721 acggcgcgct cggcgttgac ggccagagcc tcgacgctga ggatgcccga ctcggcaccg
     6781 gtcttggcgg tgcgctgagc ggtggtcgcg gtccgcttgg cggcgctgct ggtgctgcga
     6841 cgggtgttgc gcgtgttgcg ctgcgcggtc gtcgcggtgc ccttggcggc cgagcgggtg
     6901 ctcttcgcac cgctggtcgc ggcgttgcgc ttgcgggtgg cggcggcctt cttggcggcg
     6961 gcctttcgct gggttgcggt ctgctctgcc atgtggttct tacctctcct tcaccgggat
     7021 aactcgaagc ttattacttc taagttcaat acaactatac catcgaacac ctgtacgtaa
     7081 accccctcga gggcctcgtt acctgtgatg tccgtcacag aggagctagg ccgcgatgcg
     7141 ggcggtccag cgcgccgggc ggctgagctc gccggcgctg gggagcgagg cgggcgactc
     7201 ccagacacgg ttcaggaggg cggttccgcc ctcggcggcg acggcgtcgc agaacgcctt
     7261 gccctgccgg tactggcgca tcttcatgtc catgcccagc agccgcatca ggatccgctg
     7321 gggcgcggag cggtcgtcgc ggcgctgttc catggcgtcg cgcagccctt cgtgatgggg
     7381 caccagctcg ggtgcgagcg cgtccatgac gtgctcggcg tggccctcga ccacggccat
     7441 ggccacctgc acccgctcca tgatctcccg ctgggcgggg ctctggacca gcgcggccag
     7501 gccgccctcc tggaatcggg ccaccagctc gccggggttg ggcagcgacg gcgcgccgcc
     7561 cgccgccccg ctggagatct ggacgtccac ggtctccagg tagtccccca gcaggccgcc
     7621 caggtgctcg cgcagccacg gcacgccccc gaactggaac gcgtgcacca gctcgtggat
     7681 ggtcacccag cgcaggaagc tctcgcggtc ggcgcccagc ttcgccgcgg cctggtccag
     7741 gttgggcgcc accatcagca gccgcgggcg cgggtgcagc gccaggagcg agagctcgta
     7801 ctggcccagc acgtgctggg ccatgaagcc ggtgaccagc ccgacctcgg ccgccatggt
     7861 cacgccggtc ccggcgcgca gcgcgcccgc gaacggcccg gccgcgtcga agcggtgctc
     7921 catccggccg gcgaccgggt ccaacaggtc ggacagggtg gccaggttgg cctcggccca
     7981 ctcggcccgg ccgaccacct ccaccggcgg caccgggcca tcgggggcca ggccggtgtg
     8041 cgcgcccacc ggcgcctcca gctcgcgcgc cagcgccggc aggtccacgc ccagatccgg
     8101 catctggccc gggcgcgagg cgaagcgcgc gatctgacgg gccacgcccc agtcgatcac
     8161 aggtccggca gctcctggtg cgtctcgagc acgccggcga acaggtcgcc gtgctcctcg
     8221 atccgctcca gcacgtcggc cgactcgaac accaggtcgg ccgcgccgcc cccggcgccg
     8281 tggtcgacct cgtcccagct gaggggggtg gacacggtcg gtcgcgggcg cgcgcgcagg
     8341 gaataggggc cgacggtggt cttgtgccgg tcgttctggc tccagtcgat gaagaccttg
     8401 cccttgcgga cctccttctt catgaccgag acgatcaggt cgtggcgctg ttccagcacc
     8461 ttggccacgc cgtgcgcgaa cggcttgacg tcgtcgtagc ccgcgcccgt gttcagcggc
     8521 acgtacacct gcatgccctt cgagcccgag gtcttgggat ggcactccag gcccaggtgg
     8581 tccagcgtgt cgcgcaggat cagggcgacc tcacagcact cgcccagcgc ggccggcgcg
     8641 cccgggtcca ggtcgaaggc catgaccgtc ggcgaatcgg ggtcctcggc cagcgccagc
     8701 gacgggtgca gctccaggtc ggccaggttg gccagccaca ccatcgtggg gaggtcgtcg
     8761 cacaggacgt agtcgatcac gcggtggtcg ccacggcggc cgctctggat cggcatcgac
     8821 cgcacccact cgggcgcgtg cgagggcctc tgcttctcgt agaagtactt gtcgtccacc
     8881 ccgttggggt agcgcttgag cgtcagcgcg cgcccgcgca ggtggttcag caggaccggc
     8941 gccgcgcgcg tgtagtagtc gatcacctgg cccttggtga acccggcctc ggggtacagc
     9001 accttctcca ggttggagag gcgcagcttg cgcccctgga cgtcgacctc gacggccttg
     9061 gccgccacct acttcttcgc ggtcgacttg gccttggact tcggcttggc cttggacttc
     9121 gagccgttgg aggaggagga cttggccttg gccttcggct tgggctgcgt cttcggcttg
     9181 ctctggcgct tggagccgga gatcgacgcc tcgagggcgg ccatcaggtc cggcacctcg
     9241 tcgggctcct cgtccggcgg ggccgagacc tcgatctcgt ggccctcggc cttgcgctcg
     9301 atcatctcga tcacggcgtc gcggtactcg tcgcggtact ggctggggtc gaactcggtg
     9361 gtcagcgact cgatcagctg ctgggccatg tcgacctcct tcttggagac cttggccttc
     9421 ttgccctcgg gcatgtcggc cacgtcctcg ggcgacacca cctcgtcgga gaacagcatg
     9481 gtctccatgg ccaggatgcc ctcgcgcggg cggatggcca ccagctgctc cttcgagcgg
     9541 atgaccacgc gggcgatgcc caccttctcc gaggcctcca tggcgtcgta gagcagcttg
     9601 taggccttct cggcgccggt gccgggcgcc aggtagtacg ggtggtcgta gtagatcggg
     9661 tcgatctcgt gcaggtcgac gaagtcctgg atgtcgatcg tgcgcgtctt ctcgggctgg
     9721 agcgagtcca gctcggcggg ctcgatgatc acgtagcggt cggggctgat ctcgtagccc
     9781 ttgaccaggc ggtcgtacgg gacctcctcg ccgctgtccg ggttcacccg cttctgctgg
     9841 atgcgcgtgt tgtccgcgcc gtcgagctgg ttgaagcgca ccgtcttgcg ctgcaccgcg
     9901 gagtacagct tcacggggat gttcacgagg ccgaaggaaa tcgccccact ccagatcgct
     9961 cgagccatag gcctaaagct acccggagcc gccggttatc cctcttcccg cacgacctct
    10021 tgcgcgtcca tgtcctcgcg cagcccccgg aacaccggcg cccgcagcgt gcccgtgcgg
    10081 gtccactcgc cgaactccac gtgggccacg agctcgggct cgacgaactg cgtgcccttg
    10141 ggcggctggc ggcccgcgaa cggggactcg cccgtggcca ggggccccag cgcggcgacg
    10201 gtcgaggcca gcgtggcctg ggtgaagccg ctgccgacct tgccggcgta g
//
LOCUS       DASUPW010000205        10025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1850976, whole genome shotgun sequence.
ACCESSION   DASUPW010000205 DASUPW010000000
VERSION     DASUPW010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 10025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10025
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1850976"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VFY44_04770"
     CDS             <1..231
                     /locus_tag="VFY44_04770"
                     /inference="COORDINATES: protein motif:HMM:NF016054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909783.1"
                     /translation="YTGDSVPAGPDLELVAALDGAVGVPVVAEGRYASPQQVRDAFLA
                     GAHAVVVGTAITDPMALTRRLAGATPARGRPA"
     gene            228..983
                     /locus_tag="VFY44_04775"
     CDS             228..983
                     /locus_tag="VFY44_04775"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX5909784.1"
                     /translation="MSTSRHRPPALPRRLAGDSPKGAQLREILEGLLSVLPAGSALPS
                     ERELAERYGVARMTVRGELDRLTGDGLAYRIQGRGTFVSEPRVAQANALTSFTEDMRA
                     RGLEPSSRLIAREEHPAGGRMARRLELRPSAPLVRVDRLRLADAEPLAVEQVTLPLER
                     FPEAATADLAATSLFELLEERWDARPAEADQRVLAVPVQGPDAELLGVQAGHPGLRFE
                     SLARDHAGLPLYFAVSLFRGDRYEIDLRQERPS"
     gene            980..1996
                     /locus_tag="VFY44_04780"
     CDS             980..1996
                     /locus_tag="VFY44_04780"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX5909785.1"
                     /translation="MSTLMAADMEAQPEVLRGLAGRRAEVLERLGRHSGTLRGTIIVA
                     RGSSDYAAVFGRYVLEAATGRPVALAAPSLTTLYGVRTDLEGWLAVGVSQSGRTPEIV
                     SVLERYRDAGALTVAVTNEAASDLSGAAHETVELGAGDERAIPATKTFTAQLAAFALI
                     AEAWGTPPWREADWDALPEAVRHVLADPAPAERAAERVGDAAEMVCVARGYLTAVALE
                     AALKLREAAGVMAEGWSAADFRHGPIAAARPGVPALAVSAAGPAAADVDELAATLASG
                     GATVLRLSGGPAADLPFPASPAEPLAAIPAAVRAQQLALAVALRRGADPDSPAGLSKV
                     TPTR"
     gene            complement(1986..3095)
                     /locus_tag="VFY44_04785"
     CDS             complement(1986..3095)
                     /locus_tag="VFY44_04785"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5909786.1"
                     /translation="MLRLGVGAALVDGVTVEGDVEIQDGVVAQVALTPAGTGIAVPGF
                     VDAHINGYAGVDFLRTDLDGYRHVRGALAATGVVALQPTFVTSAPEAYEPASRVAAEA
                     AAAGRDGLPLVLGLHLEGPFLSPAWPGAHDPEHLRAPDAALLDELLAPGGVTTVTLAP
                     ELDGALELVDTLAARGVLVSCGHSDADAAQAHAAFDRGARAITHVHNAHRRWRPRDPG
                     LGGVALARPDVHVQAIVDGVHLAPETEYGAFLAAGTRFVMVTDAVEAAADQGGRHMLG
                     DRPIEVRDGAVRLPDGTLAGSVATLDASVRRLVAQGASLASAVHAASTAPARMLGRPD
                     LGALRPGAPAHIAVLDDRLAVTRTLVAGAEAFATA"
     gene            3174..3551
                     /locus_tag="VFY44_04790"
     CDS             3174..3551
                     /locus_tag="VFY44_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909787.1"
                     /translation="MIARRSAAAAACAAALALVSCGGSDEPARPGGKGQAPDPNDKRA
                     VALDCIVNKEQLQAKLVGEQSIQVGAEGGPRIEFLISGGEAEGRQFQGDAQGAEQIGS
                     TLLFLNDGTEDQIEKLEKCLDDQ"
     gene            complement(3564..5744)
                     /locus_tag="VFY44_04795"
     CDS             complement(3564..5744)
                     /locus_tag="VFY44_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5909788.1"
                     /translation="MSGREAAASEGSEAPKPKQAGAGLEATSLPWAFLHLAVLTAFAL
                     GQPLFDLLGKNPEFFAARGSSGGDIVTFALLVVVGPPLIGVLIEFLIGLVSKPARQVT
                     HLVFITLLTALGVIQFLKDSFSGTFVLIALSLAVGALLAFGYSRAEPVRSFLSVLSPA
                     PLLLLILFLFASPVSDIVTAGDAEAKDVKGGSNAPIVFLGLDEFPGISIMDAQGKIDA
                     KRFPGFAELAKNSTWFPNAHSIYDSTSRAWPAIMDGNYPEKDKRLPTSADHPNSIFAI
                     LGKSHSMNVSEEATTVCPRDLCEDTRLDEPYFDRIKSMTDDLGLVYQHMIAPPGVEKD
                     LDTVSETWGDFGGGGGGAGGSGAGAAPASGSEQKGDGGKKTLENLRGGRTERLDEWIA
                     SIKDTRRPTLNFKHLLLPHVPWQYLPDGRTYSPAGTDPIPQISRQSYKDQGQVDVLQQ
                     RHLLQVGFVDSEIQRLIAHLKKEGLWDKAMVVVTADHGVSFKKDQFDRRKANDTNVDE
                     LAPVPLFIKAPGQEKGRVNRENVETTDVLPTMLDILNIESPEKTDGKSAYSAAVRNRD
                     EFKMLKRDLSGWIKMPESEFEQRKTEKTRQRVAKYGEGKDGPGRIYRIGPNQQLIGQK
                     AQSAGTSQSKVTLTAPRAYANVNPDGATLPTWVTGRVSGGTQVDIAVAVNGTVRAVGN
                     TFKLATGGGQLIGVMVPENSFKKGRNTVEVFQVENGRLLKMGGS"
     gene            complement(5744..7213)
                     /locus_tag="VFY44_04800"
     CDS             complement(5744..7213)
                     /locus_tag="VFY44_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909789.1"
                     /translation="MTTPSDTSQDALVNAESGSFRDPESRIFYADGAVYRALSPDGLA
                     DFEALTETKLFDAAVADGRLVGTERVEDTGSLPDLLVHERAGVLRHERIPFVSYPYEW
                     PFSMLKDAALLQLDLLEASLKEGLTLKDASSYNVQFRGAQPAFVDVGSFERRREGEGW
                     VGYRQFCMLFLYPLMLQANKDVPFHGILRGQIDGISPQQMRNLSSFRDRFRKGYPSNV
                     FIHARLEAKYADRAAEVKGEMKRANIKKEILLAQVSKLRKVVQGLEWNPPQGVWVAYG
                     ERNSYTDSDAERKDEFVREVSTSQRWPLTWDIGANNGRYSRIAAEGSDYVLALDADQG
                     PMELLYRGLRDEGAKNILTLTMNLADQSPNLGWRGAERKDLAGRGKPDLVLALALIHH
                     TTVTANVPIAEFVDWLASLGTSLVIEFPTREDPMVKKLLSGKREGLHPDYERGNFERV
                     LAGAFDVERRERLASGTRLLYFARPKGSGSPIDLTATEG"
     gene            complement(7329..7520)
                     /locus_tag="VFY44_04805"
     CDS             complement(7329..7520)
                     /locus_tag="VFY44_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909790.1"
                     /translation="MSTVIAYLDAGTGSQIAALVAGGFAAFAVTAKMYWNRVLVFLHI
                     RKPEDETATTATAEGPDTK"
     gene            7650..8693
                     /locus_tag="VFY44_04810"
     CDS             7650..8693
                     /locus_tag="VFY44_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5909791.1"
                     /translation="MKLIIQIPCKDEEAQLPGTLADLPREVPGFDVVEWLVIDDGSTD
                     RTIEVARTGGVDHIVKLTNNKGLAAGFQAGLDACLKLGADVIVNTDADNQYYGGDIHK
                     LVEPIIARSADMVVGDRETDTIEHFSPLKKRLQRLGSAVVRRASGTNVPDTTSGFRAY
                     NREAALQMVVVSRFTYTLETIIQAGKTLVAVDHVPIRTNPQTRESRLFPSMWSYVRRN
                     SVAIFRVYALYEPLRVFMIAAGIVALPAAVIWARFIYFFLIGEGEGHVQSLILGSTLL
                     IIAVIFVALGVIGDLLAASRSMQQRTLERVRRVELSLGVEPSHYEPAKSPGAGTTGAD
                     SGTATGKREEVRL"
     gene            8690..9328
                     /locus_tag="VFY44_04815"
     CDS             8690..9328
                     /locus_tag="VFY44_04815"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5909792.1"
                     /translation="MSSGVATPGSTDPGTAPTGNTFDKYGSTNPVVRRRMARFHGTLD
                     ELWHQAAPASVLDVGCGEGVLTHEWAERLGDKRIVGIDLDDPGLQAEWATRQRPNLEY
                     RIEEATALPFADGEFELATAIEVLEHVPEPEATVAEMARVASRHLLVSVPREPMWRGL
                     NMARGAYIKDLGNTPGHVNHWSKRSFVKLLSRHGEVVEARSPFPWSMLLVRL"
     gene            9337..>10025
                     /locus_tag="VFY44_04820"
     CDS             9337..>10025
                     /locus_tag="VFY44_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909793.1"
                     /translation="MAEAEEGGYQKGAALLSLGIGTTGLVTYGYFALASHNLTEAQYG
                     GITLLWSAVFITVSVLYRPVEQLLSRTIAERDANGVTGNQHLRVAATIQLALGIVFVI
                     AALALKGPIQGDLFNGSTTLYRVLIVAVLAYAVSYFARGFLAGRRRFGLYGALVLMEA
                     CSRVLFAVAVAVGIAEGQSVVALGMAAAPIVSLAVVPLALSRTRAPASAPVMTEEEAS
                     AEARHLDEVSA"
BASE COUNT         1338 a         3593 c         3662 g         1432 t
ORIGIN      
        1 tacaccggcg actcggtgcc tgcggggccc gacctggagc tggtggcggc cctggacggc
       61 gccgtcggcg tgcccgtggt ggccgagggc cggtacgcgt ccccgcagca ggtgcgcgac
      121 gcgttcctgg ccggcgcgca cgccgtggtg gtgggcacgg cgatcaccga cccgatggcc
      181 ctgacgcggc ggctggccgg ggcgaccccg gcgcgggggc ggccggcgtg agcacctcgc
      241 ggcaccggcc cccggccctg ccccgccggc tggccggcga cagccccaag ggcgcgcagc
      301 tgcgcgagat cctggaaggc ctgctgtccg tgctccccgc cggcagcgcg cttccgtccg
      361 agcgcgagct ggccgagcgc tacggcgtgg cgcgcatgac ggtccgcggc gagctggatc
      421 gcctgaccgg tgacgggctg gcctaccgga tccagggccg cggcaccttc gtgtccgagc
      481 cccgcgtggc ccaggccaac gcgctgacct cgttcaccga ggacatgcgc gcccgcgggc
      541 tggagcccag ctcccggctg atcgcgcgcg aggagcaccc cgccgggggc cggatggcgc
      601 gccggctcga gctccgcccc tccgccccgc tcgtgcgcgt ggaccgcctg cgcctggccg
      661 acgccgagcc gctggcggtc gagcaggtga cgctgccgct ggagcgcttc cccgaggcgg
      721 cgaccgcgga cctggccgcc acgtcgctgt tcgagctgct cgaggaacgc tgggacgcgc
      781 gcccggccga agccgaccag cgcgtgctgg ccgtgcccgt gcagggcccc gacgccgagc
      841 tgctgggggt ccaggccggc caccccgggc tgcgcttcga gtcactggcc cgcgaccacg
      901 cggggctgcc gctgtacttc gccgtgtccc tgttccgagg cgaccggtac gagatcgacc
      961 tgcgccagga gcggccctcg tgagcacgct catggccgcc gacatggagg cgcagcccga
     1021 ggtgctgcgc ggcctggccg ggcgccgcgc ggaggtgctg gagcgcctgg gccggcactc
     1081 gggcacgctg cgcggcacga tcatcgtcgc ccgcggctcg tccgactacg cggcggtgtt
     1141 cggccgctac gtgctcgagg ccgccaccgg ccgcccggtg gcgctggccg caccgagcct
     1201 gaccacgctc tacggcgtcc ggaccgatct cgagggctgg ctggcggtgg gcgtcagcca
     1261 gtcgggccgg acgccggaga tcgtctccgt gctggagcgc taccgcgacg ccggcgcgct
     1321 gaccgtggcg gtcaccaacg aggccgccag cgacctgtcc ggcgccgcgc acgagaccgt
     1381 ggagctgggc gccggcgacg agcgcgcgat ccccgccacg aagacgttca ccgcccagct
     1441 ggcggcgttc gcgctgatcg ccgaggcgtg gggcacgccg ccgtggcgag aagccgactg
     1501 ggacgccctg cccgaggcgg tccggcacgt gctggccgat cccgcgcccg ccgagcgcgc
     1561 cgcggagcgg gtgggcgacg ccgccgagat ggtgtgcgtg gcccggggct acctgaccgc
     1621 ggtcgcgctg gaggcggcgc tcaagctgcg cgaggcggcc ggcgtgatgg ccgagggatg
     1681 gtcggccgcc gacttccgcc acggcccgat cgcggccgcc cgtcccggcg tgccggccct
     1741 ggccgtcagc gccgccgggc ccgcggcggc cgacgtggac gagctggcgg ccaccctggc
     1801 ctccggcggc gccaccgtcc tgcgcctgtc cggcggcccg gccgccgacc tgccgttccc
     1861 ggcctcgccc gccgagccgc tggctgccat acccgccgcg gtgcgcgccc agcagctggc
     1921 cctggcggtg gcgctgcggc gcggcgcgga ccccgacagc ccggccgggc tgtccaaggt
     1981 cacccctacg cggtagcgaa cgcctcggcg ccggccacca gcgtccgggt caccgcgagc
     2041 ctgtcgtcca gcaccgcgat gtgggcgggc gcccccgggc gcaacgcgcc caggtccggg
     2101 cggcccagca tccgggcggg cgcggtggag gcggcgtgca cggcggaggc cagcgaggcg
     2161 ccctgggcca ccagccggcg cacggaggcg tccagcgtgg ccacgctgcc ggccagcgtc
     2221 ccgtcgggga gccgcaccgc gccgtcgcgc acctcgatcg gcctgtcgcc cagcatgtgg
     2281 cggccgccct ggtcggccgc cgcctcgacg gcgtcagtga ccatgacgaa gcgcgtgccc
     2341 gccgccagga aggcgccgta ctcggtctcg ggcgccaggt gcaccccgtc gacgatggcc
     2401 tggacgtgca cgtcgggccg cgccagcgcc accccgccca gaccggggtc gcgcgggcgc
     2461 cagcggcgat gggcgttgtg cacgtgggtg atggcgcggg cgccgcggtc gaaggcggcg
     2521 tgcgcctggg ccgcgtcggc gtcggagtgg ccgcaggaga ccagcacccc gcgggccgcc
     2581 agcgtgtcca ccagctccag cgcgccgtcc agctcgggcg ccagcgtgac cgtggtcacc
     2641 ccgcccggcg ccagcagctc gtccagcagc gccgcgtcgg gcgcccgcag gtgctcgggg
     2701 tcgtgtgcgc ccggccacgc gggcgacagg aacgggccct ccaggtgcag gcccagcacc
     2761 agcggcagcc cgtcgcggcc ggccgcggcc gcctccgcgg cgacccgcga cgcgggctcg
     2821 tacgcctcgg gcgccgaggt cacgaacgtc ggctgaagcg cgacgacgcc ggtggcggcc
     2881 agcgcgccgc gcacgtggcg gtagccgtcc aggtcggtgc gcaggaagtc gacgcccgcg
     2941 tagccgttga tgtgggcgtc cacgaacccg ggcacggcga tgccggtgcc ggcaggcgtc
     3001 agcgccacct gggccaccac cccgtcctgg atctccacgt cgccctcgac ggtcacgccg
     3061 tcgaccagcg ccgcgcccac tcctagccgc aacacgccct cctgactggt atagaccagc
     3121 gcagtctaga cccgcccggt cgctcccccg ccgcagccac taccctgccc gcgatgatcg
     3181 ctcgacgctc cgcggcggct gccgcctgtg ccgccgccct cgccctggtc tcgtgcgggg
     3241 gaagcgacga gcccgcgcgc cccggcggga agggccaggc gcccgacccc aacgacaagc
     3301 gcgcggtggc gctggactgc atcgtcaaca aggagcagct ccaggccaag ctggtcggcg
     3361 agcagtcgat ccaggtcggc gccgagggcg gcccccggat cgagttcctg atctccggcg
     3421 gcgaggccga gggccgtcag ttccagggcg acgcccaggg cgcggagcag atcgggtcga
     3481 cgctgctgtt cctcaacgac ggcaccgagg accagatcga gaagctcgag aagtgcctgg
     3541 acgaccagta gagcgtcagc cgctcagctg ccgcccatct tcagcagccg cccgttctcg
     3601 acctggaaca cctcgacggt gttcctgccc ttcttgaagc tgttctcggg caccatcacg
     3661 ccgatcagct gaccgccgcc ggtggccagc ttgaaggtgt tgcccaccgc gcgcacggtg
     3721 ccgttgaccg cgacggcgat gtccacctgc gtgccaccgg agacgcggcc ggtgacccag
     3781 gtcgggaggg tcgcgccgtc cgggttcacg ttcgcgtagg cgcgcggggc ggtcagggtg
     3841 accttcgact gtgacgtgcc cgcggactgc gccttctggc cgatcagctg ctggttgggg
     3901 ccgatgcggt agatccggcc cgggccgtcc ttgccctcgc cgtacttggc cacgcgctgg
     3961 cgggtcttct cggtcttgcg ctgctcgaac tcggactccg gcatcttgat ccagcccgag
     4021 aggtcgcgct tgagcatctt gaactcgtcc cggttgcgca cggcggccga gtacgccgac
     4081 ttgccgtcgg tcttctcggg ggactcgatg ttcaggatgt ccagcatcgt gggcagcacg
     4141 tcggtggtct ccacgttctc gcggttgacc ctgcccttct cctggccggg ggccttgatg
     4201 aacagcggca cgggcgcgag ctcgtccacg ttggtgtcgt tggccttgcg gcggtcgaac
     4261 tggtccttct tgaacgagac cccatggtcg gcggtgacca cgaccatcgc cttgtcccac
     4321 aggccttcct tcttgaggtg cgcgatcagc cgctggatct cgctgtccac gaagcccacc
     4381 tgcagcaggt ggcgctgctg gagcacgtcg acctgcccct ggtccttgta ggactggcgc
     4441 gagatctggg ggatcgggtc ggtgcccgcc gggctgtagg tgcggccgtc gggcaggtac
     4501 tgccagggca cgtgcggcag cagcaggtgc ttgaagttca gcgtcggccg gcgggtgtcc
     4561 ttgatcgacg cgatccactc gtccagccgc tcggtgcggc cgccgcggag gttctcgagc
     4621 gtcttcttgc cgccgtcgcc cttctgctcg gaccccgatg cgggcgcggc accggcgccc
     4681 gagccgccgg ctcccccgcc cccgccgccg aagtcacccc acgtctccga gacggtgtcc
     4741 aggtccttct cgacgccggg cggcgcgatc atgtgctggt agaccaggcc caggtcgtcg
     4801 gtcatcgact tgatccggtc gaagtagggc tcgtccaggc gcgtgtcctc gcacaggtcg
     4861 cgcgggcaga ccgtggtggc ctcctcgctg acgttcatcg agtggctctt gccgaggatc
     4921 gcgaagatcg agttgggatg gtcggccgag gtggggagcc gcttgtcctt ctcggggtag
     4981 ttgccgtcca tgatggccgg ccacgcgcgg ctggtggagt cgtagatcga gtgcgcgttg
     5041 gggaaccagg tgctgttctt cgccagctcg gcgaagcccg ggaaccgctt ggcgtcgatc
     5101 ttgccctggg cgtccatgat cgagatgccg gggaactcgt ccaggcccag gaacacgatc
     5161 ggcgcgttcg atccgccctt gacgtccttg gcctcggcgt cgccggcggt gacgatgtcc
     5221 gacaccggcg aggcgaacag gaacaggatc agcagcagca gcggcgccgg cgagagcacg
     5281 ctgaggaagg agcgcaccgg ctcggcgcgc gagtagccga acgccagcag ggcgccgacg
     5341 gccagcgaca gcgcgatgag cacgaaggtg ccgctgaacg agtccttcag gaactggatc
     5401 actcccagcg ccgtgagcag cgtgatgaac accaggtgcg tcacctgacg cgcgggcttg
     5461 gacaccaggc cgatcaggaa ctcgatcagc acgccgatca gcggcgggcc gacgaccacc
     5521 agcagggcga aggtgacgat gtcgcccccg gacgatccgc gggcggcgaa gaactcgggg
     5581 ttcttgccca gcaggtcgaa cagcggctgg cccagcgcga acgcggtcag caccgccagg
     5641 tgcaggaagg cccagggcag cgaggtggcc tcgaggccgg cgcctgcctg cttgggcttg
     5701 ggggcctcgg atccttccga ggcggcggcc tctcggccgc tcatcagccc tcggtcgccg
     5761 tcaggtcgat cggcgagccg ctgcccttgg gacgggcgaa gtacagcagc cgggtgcccg
     5821 aggccagacg ctcacgacgc tcgacgtcga aggcgccggc cagcacgcgc tcgaagttcc
     5881 cgcgctcgta gtcggggtgc agcccctcgc gcttgccgct gagcagcttc ttgaccatcg
     5941 ggtcctcgcg cgtggggaac tcgatgacca gcgacgtgcc cagcgaggcc agccagtcca
     6001 cgaactcggc gatcggcacg ttggccgtga ccgtggtgtg gtggatcagg gccagggcca
     6061 gcaccaggtc gggcttgccg cggccggcca ggtccttgcg ctcggcgccg cgccagccca
     6121 ggttgggcga ctggtcggcc aggttcatgg tcagcgtcag gatgttcttg gcgccctcgt
     6181 cgcgcaggcc gcggtagagc agctccatcg ggccctggtc ggcgtccagc gccagcacgt
     6241 agtccgagcc ctcggcggcg atgcgcgagt agcggccgtt gttcgcgccg atgtcccagg
     6301 tcagcggcca gcgctggctg gtggacacct cgcgcacgaa ctcgtccttg cgctcggcgt
     6361 cggagtcggt gtagctgttg cgctcgccgt aggccaccca gacgccctgc ggcgggttcc
     6421 actccagccc ctggacgacc ttgcgcagct tggagacctg ggccagcagg atctccttct
     6481 tgatgttcgc gcgcttcatc tcgcccttga cctcggcggc gcggtcggcg tacttggcct
     6541 ccaggcgcgc gtggatgaac acgttgctgg ggtagccctt gcggaaccgg tcgcggaagc
     6601 tggacaggtt gcgcatctgc tgcgggctga tgccgtcgat ctggccgcgc aggatgccgt
     6661 ggaagggcac gtccttgttg gcctggagca tcagggggta caggaagagc atgcagaact
     6721 ggcggtagcc cacccagccc tcgccctcgc gccggcgctc gaagctgccg acgtcgacga
     6781 aggcgggctg cgcgccgcgg aactggacgt tgtacgacga cgcgtccttg agggtcaggc
     6841 cctccttcag cgacgcctcg agcaggtcga gctgcagcag cgcggcgtcc ttgagcatcg
     6901 agaacggcca ctcgtagggg taggagacga acgggatgcg ctcgtgcctc agcacgcccg
     6961 cgcgctcgtg gaccagcagg tcgggcagcg aacccgtgtc ctcgacgcgc tcggtgccca
     7021 ccaggcggcc gtcggccacc gcggcgtcga acagcttggt ctcggtcagg gcctcgaagt
     7081 cggccaggcc gtcgggcgac agcgcgcggt agaccgctcc gtccgcatag aagatgcggc
     7141 tctcggggtc acggaaggac cccgactcgg cgttgacgag ggcgtcttga gaagtgtcgc
     7201 tcggcgtagt cattcagagg tgttcgggcc tgcgcccgag gcgttcagcg ggctgccagg
     7261 gcggcagcgc gaacggaagc gtagctggcg gggagcgcgc gggtagtgcg ccctccccgc
     7321 cggaccggct acttggtgtc cggaccctcg gcggtcgccg tggtcgcggt ctcgtcctcc
     7381 ggcttgcgga tgtggaggaa gaccagaacg cggttccagt acatcttggc ggtgacggcg
     7441 aacgcggcga aaccgccggc cacgagggct gcgatctggc tgccggtgcc ggcgtcgaga
     7501 taggcgatga cagtgctcat aaaagattga ctccgtgacc gttaagggtt caggacagcg
     7561 gcccaaggta gcaaaggacg ccggcggcgt acgggccttt acatggatgt gaagccaccc
     7621 gcaggcgctg ggctaccctc tcccgcgacg tgaagctcat catccagatc ccctgcaagg
     7681 acgaagaggc ccagctaccg ggcacgctgg ccgatctccc gcgcgaggtt ccgggcttcg
     7741 acgtcgtcga atggctggtg atcgacgacg ggtccaccga ccgcacgatc gaggtcgcgc
     7801 gcacgggcgg cgtggaccac atcgtgaagc tgaccaacaa caagggcctg gccgccggct
     7861 tccaggcggg cctggacgcc tgcctgaagc tgggcgccga tgtgatcgtg aacacggacg
     7921 ccgacaacca gtactacggc ggcgacatcc acaagctcgt ggagccgatc atcgcccgca
     7981 gcgccgacat ggtcgtgggc gaccgcgaga ccgacacgat cgagcacttc tcgccgctga
     8041 agaagcggct gcagcggctg ggcagcgcgg tcgtgcggcg cgcctcgggc accaacgtcc
     8101 ccgacaccac gtcggggttc cgcgcctaca accgcgaggc cgcgctgcag atggtggtgg
     8161 tctcgcgctt cacctacacg ctggagacga tcatccaggc cggcaagacg ctggtggccg
     8221 tggaccacgt gcccatccgg accaacccgc agacgcgcga gtcgcgcctg ttcccgtcga
     8281 tgtggtccta cgtgcggcgc aacagcgtcg cgatcttccg cgtgtacgcg ctgtacgagc
     8341 cgctgcgcgt gttcatgatc gccgcgggca tcgtggccct gcccgccgcg gtgatctggg
     8401 cccggttcat ctacttcttc ctgatcggcg agggcgaggg ccacgtccag tcgctgatcc
     8461 tgggctccac cctgctgatc atcgccgtga tcttcgtggc gctgggcgtg atcggggacc
     8521 tgctggccgc gtcgcgctcg atgcagcaac gcaccctgga gcgcgtgcgc cgcgtcgagc
     8581 tcagcctggg tgtcgagccc tcccactacg agccggccaa gtcccccggc gcgggcacca
     8641 cgggcgcgga ctcgggcacg gccaccggca agcgggaaga ggtgcgcctg tgagctccgg
     8701 cgtcgccacc cccgggtcga ccgatcccgg caccgccccc acgggcaaca cgttcgacaa
     8761 gtacggctcc acgaaccccg tcgtgcgccg ccggatggcg cgcttccacg gcacgctcga
     8821 cgagctttgg caccaggccg cgccggcctc ggtgctggac gtgggctgcg gcgagggcgt
     8881 gctcacgcac gagtgggccg agcgcctggg cgacaagcgg atcgtcggca tcgacctgga
     8941 cgaccccggg ctccaggccg agtgggccac ccgccagcgc cccaacctgg agtaccggat
     9001 cgaggaggcc accgcgctgc cgttcgccga cggcgagttc gagctggcca ccgcgatcga
     9061 ggtgctggag cacgtgcccg agcccgaggc caccgtcgcc gagatggccc gtgtggccag
     9121 ccgccacctg ctggtgtcgg tcccgcgcga gcccatgtgg cgcgggctga acatggcccg
     9181 cggcgcctac atcaaggacc tgggcaacac gcccggccac gtgaaccact ggtccaagcg
     9241 ctcgttcgtg aagctgctct cgcggcacgg tgaagtggtg gaggcgcgct cgcccttccc
     9301 ctggtccatg ctgctcgttc gcctgtaagg ggccccatgg ccgaggcaga ggaaggcggc
     9361 taccagaagg gcgccgcgct gctgtccctg ggcatcggga ccacggggct ggtcacctac
     9421 ggctacttcg cgctggcctc gcacaacctg accgaggcgc agtacggcgg gatcacgctg
     9481 ctgtggtcgg cggtcttcat cacggtctcg gtgctctacc gcccggtcga gcagctgctg
     9541 tcgcggacga tcgccgagcg cgacgcgaac ggcgtgacgg gcaaccagca cctgcgcgtg
     9601 gccgcgacga tccagctggc gctgggcatc gtgttcgtga tcgccgcgct ggcgctcaag
     9661 gggccgatcc agggcgacct gttcaacggc tcgaccacgc tctaccgcgt gctgatcgtg
     9721 gccgtgctgg cctacgcggt cagctacttc gcgcgcgggt tcctggccgg ccggcgccgg
     9781 ttcggcctgt acggcgcgct ggtgctgatg gaggcctgct cgcgcgtcct cttcgccgtg
     9841 gccgtcgcgg tcggcatcgc cgagggccag tccgtggtgg cgctgggcat ggcggccgcg
     9901 ccgatcgtct cgctggcggt ggtgccgctg gcgctctcac gcacgcgcgc gccggcgagc
     9961 gcgcccgtga tgaccgagga ggaggcgagc gcggaggcgc gccacctgga cgaggtgtcg
    10021 gccat
//
LOCUS       DASUPW010000206         2898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1851052, whole genome shotgun sequence.
ACCESSION   DASUPW010000206 DASUPW010000000
VERSION     DASUPW010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1851052"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..1186
                     /locus_tag="VFY44_04825"
     CDS             212..1186
                     /locus_tag="VFY44_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX5909794.1"
                     /translation="MLTRSIRLAPLLLFLLAGACGQPAEPPVGGPDEACTVPTVNDAP
                     APASALAAFAYDRAAAYDPVLQPVGTQPGVAIYALSFLSPRGTGRATGLLFVPEGAGP
                     FAGLLLQHGLPGNAQQMTGEAVVLARQGAVVVALDAPFARRGGSPVRFTAADSAEQVQ
                     LMTDLQRAVDLLVARPDVDASRIGYMGVSYGGAMGALFVGIERRLRTGILVVADGGLV
                     SHFTGPEDGGGPVDGLPCAQRQRWLESMIPIEPIKYVGFAPPTPLLLQSGRQDNLVPP
                     RAAELLHAAVRDPRTIRWYEAGHGLTAEAARDRLLWLQQSLGMRAPAS"
     gene            1320..2390
                     /locus_tag="VFY44_04830"
     CDS             1320..2390
                     /locus_tag="VFY44_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5909795.1"
                     /translation="MKTTKALFPVAAVAASALLWAACTHGTQLVSGGTTIDRDDIAGE
                     VTDALAGSQAVPTLSAPPTATTGVQPRDTAGPLLEELARIAAASKGTVGVAAIDLATG
                     DVTAFNADERFAMASTFKVPVAVYALRLAQEGRLSLTEPVPVRREDMVEPGILFEHFR
                     HPGVAVSLLNAMELSVTTSDNGATDIILGRVGGPAAVQAWLQSGGYTSMNIGRRTLRE
                     TFTPGVAPQPADAGTAGLDRTVTPREMARFLADLHRGQLLAAEGTTTILEMMERTAGD
                     RLALYLPPGTTVRHKTGTLLGGEGVSVNDVGYVTRPNGAVIVMAVFIRNSPESVAHST
                     RDRVIGHVARAIYDDIQLRAAR"
     gene            2504..>2898
                     /locus_tag="VFY44_04835"
     CDS             2504..>2898
                     /locus_tag="VFY44_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909796.1"
                     /translation="MKTTKALFAGAAVAACALVWAASTYGAPPAPGGMAIDADDIAGV
                     VRGANGPEAGVWVIAETSDLPTKLAKIVVTDHQGRYLLPDLPRASYDVWVRGYGLVDS
                     PKQRAEPGTMLDLKATPAPNARAAAEYYPA"
BASE COUNT          426 a         1011 c         1057 g          404 t
ORIGIN      
        1 cgggcggatc gcggcggtac atcaatcaga cggagctggt ggatgaagac gacggaggca
       61 cgcgtcgcgg acgcggcggt ggcggcgtcc acgcgctccg gggggcctgc gcgcgcgcat
      121 cgtggcggga cggccggacc gacggtccga ccgtcccggt tgggacagga taagcgttct
      181 ccgggtccag ctcatgcccg ggggtacagc catgctcacg cgctccatcc gcctcgctcc
      241 actgctcctg ttcctgctcg ccggcgcctg cggccagccg gcggagccgc ccgtcggcgg
      301 acccgatgag gcctgcaccg tcccgacggt gaacgacgcg cccgcacccg cctccgccct
      361 ggccgcgttc gcctacgacc gcgcggcggc ctacgacccg gtgctccagc cggtcggcac
      421 ccagccgggc gtggcaatct acgcgctgag ctttctcagc ccgcgcggca ccggccgcgc
      481 cacggggctg ctctttgtcc ccgagggtgc gggcccgttc gcgggattgc tgctgcagca
      541 cggattgccg ggcaacgccc agcagatgac gggcgaggcg gtggtgctgg cgcgccaggg
      601 ggcggtggtg gtggcgctcg acgcgccatt cgcgcggcgc gggggcagcc cggtgcgctt
      661 taccgcggcc gacagcgcgg agcaggtgca gctcatgacc gacctgcagc gcgccgtgga
      721 cctgctcgtg gcgcgcccgg acgtggacgc cagccggatc ggctacatgg gcgtaagcta
      781 tggcggggcg atgggcgcac tcttcgtcgg catcgagcgg cggctcagga cggggatcct
      841 cgtggtggcc gacggcgggc tcgtctcgca cttcaccggg cccgaggacg gcggcggccc
      901 cgtggacggg ctcccctgcg cgcagcggca gcgctggctg gagtcgatga tccccatcga
      961 gccgatcaag tacgtcggct tcgcgccgcc gacgcccctg ctgctgcagt cggggcggca
     1021 ggacaacctg gtgccgccgc gcgccgccga gctgctgcac gccgcggtgc gcgatcccag
     1081 gaccatccgc tggtacgagg cggggcacgg ccttaccgcc gaggccgcgc gcgaccgcct
     1141 gctgtggctg cagcagtcgc tggggatgcg cgccccggcc tcctgagccg gcgcccgcgg
     1201 acaggcgcgg aagcgagccc gtcgcggaac tgcagcgtcg gtcgtgacaa ccggaaccgg
     1261 cacgcgttgt tcgtccagct gatccgaacg cgccggaaca acctcacggg agctggcaga
     1321 tgaagacgac gaaggcactc ttcccggttg cggccgtggc ggcctccgcg ctgctctggg
     1381 cggcctgcac gcacggcacg cagctcgtct ccggagggac gacgatcgat cgcgacgaca
     1441 tcgcggggga ggtgacggat gcgctggcgg gttcgcaggc agtacccacg ttgtctgctc
     1501 ctccgacggc gacaaccgga gtgcagccgc gggatactgc gggccctttg ctcgaggagc
     1561 tcgcgcgtat cgctgcagcg agcaagggga cggtcggcgt cgccgccatc gacctcgcga
     1621 ccggtgacgt gaccgcgttc aatgccgacg aacgcttcgc gatggcgagc accttcaagg
     1681 ttcccgtggc cgtctacgcg ctgcgcctgg cccaggaggg gcggctgtcc ttgaccgagc
     1741 cggttcccgt ccgccgcgag gacatggtcg aaccgggcat tctgttcgag cacttccgcc
     1801 atcccggggt cgccgtctcg ctgctcaatg ccatggagct gagcgtcact accagcgaca
     1861 acggcgccac cgacatcatc ctcggacgtg tcggagggcc ggcagcggtg caggcgtggc
     1921 tgcaatccgg gggatatacg tcgatgaaca tcgggcgccg cacgttgaga gagaccttta
     1981 cgcccggcgt cgcgccccag ccggcggatg ccggaaccgc cggtctcgat cgcaccgtga
     2041 ccccacgcga gatggcgcgg ttcctggccg acctgcaccg cgggcagctg cttgccgccg
     2101 aggggactac caccatcctc gagatgatgg aacgcacggc cggcgaccgc cttgcgctct
     2161 accttccgcc cggcaccacc gtgcgccaca agaccgggac attgcttggc ggtgaggggg
     2221 tgtcggtcaa cgacgtcggg tacgtcacga gaccgaatgg cgcagtcatc gtgatggcgg
     2281 tcttcatcag gaacagcccg gagtcggtcg cacactccac gcgtgacagg gtgatcggcc
     2341 acgtcgcccg cgcgatctac gacgacatcc agcttcgcgc ggcccggtaa gctcactgcg
     2401 acagcagggt gccggcgcgc gcaagcgtcg gtcatgacaa ccggaacgca tgattcgttc
     2461 agctgatccg aacgcaccgg acaaacctca agggagctgg cagatgaaga cgaccaaggc
     2521 actcttcgcg ggcgcggcgg tggcggcctg cgcgctggtg tgggcggcca gcacgtacgg
     2581 cgcgcccccc gcccccggcg ggatggcgat cgatgccgac gacatcgcgg gggtggtgag
     2641 gggggcgaac ggccccgagg cgggggtgtg ggtgatcgcc gagacctccg acctgccgac
     2701 gaagctcgcc aagatcgtgg tgaccgacca ccaggggcgg tacctcctcc cagacctccc
     2761 gcgggcgagc tacgacgtct gggtgcgcgg gtacgggctg gtcgactcgc cgaagcagcg
     2821 ggccgagccc gggacgatgt tggacttgaa ggccaccccc gcgccgaatg cgcgcgccgc
     2881 ggctgagtat tacccggc
//
LOCUS       DASUPW010000207         2743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1296101, whole genome shotgun sequence.
ACCESSION   DASUPW010000207 DASUPW010000000
VERSION     DASUPW010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296101"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /locus_tag="VFY44_04840"
     CDS             complement(<1..1245)
                     /locus_tag="VFY44_04840"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX5909797.1"
                     /translation="MPLAPRTALVAVLMLLACAAPAAARPNVVLVMTDDQTPESIRVM
                     NRTKALVGAPGTTFTNSVATFPLCCPSRATYLSGQYAHNHRVLNNVPPFGGFTRFDQA
                     NALGAWMQGAGYRTMHVGRTLNGYGTDTPDITAVPPGWNDWLVPLEPTVYNYERWRMN
                     ENGQILELPGLERPTEFQTDYLSRRATELIERTAPTRQPFFLSLTYPAPHVGRTSTVA
                     PRHRGVFASLPMPLTPAFSEADVRDKPRVIRRRPRLSLAQMGLVQGAYRQQLESLLSV
                     DEGVAQVMGALQRTGALANTLVIFTSDNGLMYGQHRIRSAKVFPYEESIRVPLMMRGP
                     GVPAGLRYGKQAGNIDLAPTILDAADALPGRLIDGVSLLDLAARPQERSGRELVIENG
                     RGLKTQSQYRGLRNDRYVYIRHD"
     gene            1274..2143
                     /locus_tag="VFY44_04845"
     CDS             1274..2143
                     /locus_tag="VFY44_04845"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5909798.1"
                     /translation="MSERGTFEAVPAPLLVLGGIATTQLGASLAQQIFDEAGPSGTVF
                     LRVAFAALVLVVLWRPRWRGHSRRDLGLALLFGLSLAFMNLTFYESLDRIPQGVAVTI
                     EFAGPLTVAVLGSRRFMDLVWVTLAAAGIVLLANLGGAGVDMTGVALALAAGVFWGSY
                     IIISARVGQTFPRMEGLALAMVFGAVVLIPFGVAGGGTALLDPHVLAVGAGVAVLSSA
                     IPYSLELEALRRLPTGTFGVLMSLEPAVAALSGLVVLGQVLDTREWAGIALVVAASAG
                     AATFATRETVARD"
     gene            2157..>2743
                     /locus_tag="VFY44_04850"
     CDS             2157..>2743
                     /locus_tag="VFY44_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX5909799.1"
                     /translation="MSHPDSFGAKAELEVGGTTHEIFRLDALQSEFDVARLPYSLKVL
                     LENLLRNEDGESVTKETIESVAKWVASDEPSIEIAYSPSRVLMQDFTGVPAIVDLAAM
                     RDAMEELGGDPTKINPLVPVELVIDHSIQVDSFAEKMAFKKNAELEFERNQERYAFLR
                     WGQTAFDNFSVVPPNTGICHQVNLEYLSRVVTARDG"
BASE COUNT          356 a          927 c         1032 g          428 t
ORIGIN      
        1 gtcgtggcgg atgtacacgt agcggtcgtt gcgcagcccc cggtactgcg actgggtctt
       61 caggccgcgg ccgttctcga tcaccagctc acgcccgctg cgctcctgcg ggcgcgccgc
      121 caggtccagc agcgagacgc cgtcgatcag ccggcccggc aacgcgtcgg ccgcgtccag
      181 gatggtgggc gccaggtcga tgttgcccgc ctgtttgccg tagcgcaggc cggcgggcac
      241 gcccggcccg cgcatcatca gcggcacccg gatcgactcc tcgtagggga agaccttggc
      301 cgagcggatc cggtgctgtc cgtacatcag cccgttgtcg gaggtgaaga tgaccagcgt
      361 gttggccagg gcgccggtgc gttgcagcgc gcccatgacc tgcgccacgc cctcgtccac
      421 cgacagcagc gactccagct gctggcggta ggcgccctgg accaggccca tctgggccag
      481 gctcaggcgc gggcggcggc ggatgacccg cggcttgtcg cgcacgtccg cctcgctgaa
      541 ggcgggcgtc agcggcatcg gcagcgaggc gaagacgccg cggtggcggg gcgcgacggt
      601 ggacgtgcgc ccgacgtgcg gcgccgggta ggtcagcgac aggaagaacg gctggcgcgt
      661 gggggccgtg cgctcgatca gctcggtggc acggcggctc aggtagtcgg tctggaactc
      721 ggtgggccgc tcgaggccgg gcagctccag gatctgtccg ttctcgttca tgcgccagcg
      781 ctcgtagttg tagacggtgg gctccagcgg caccagccag tcgttccacc ccggcggcac
      841 cgccgtgatg tcgggcgtgt ccgtcccgta gccgttgagc gtgcgcccca cgtgcatggt
      901 gcggtagccg gcgccctgca tccacgcgcc gagcgcgttg gcctggtcga agcgggtgaa
      961 gccgccgaac ggcgggacgt tgttcagcac ccggtggttg tgcgcgtact ggccgctgag
     1021 gtaggtggcc cgcgacgggc agcacagcgg gaacgtggcc accgagttgg tgaaggtggt
     1081 gcccggcgcc ccgaccagcg ccttggtccg gttcatgacc cggatcgact ccggcgtctg
     1141 gtcgtcggtc atgaccagga ccacgttggg gcgcgcggcc gccggcgcgg cacaggccag
     1201 cagcatcaga acggcgacga gcgcggtgcg cggcgcgagg ggcatccgcc ggagggtagg
     1261 tagcgtggcg gcggtgagcg agcgcggcac cttcgaggcg gtccccgcgc cgctgctggt
     1321 gctgggcggc atcgcgacga cgcagctggg cgcgtcgctg gcgcagcaga tcttcgacga
     1381 ggccgggccc agcggcaccg tcttcctgcg cgtggccttc gccgccctgg tgctggtggt
     1441 cctgtggcgc ccgcggtggc gcggccacag ccggcgcgac ctgggcctgg cgctgctgtt
     1501 cgggctctcg ctggcgttca tgaacctgac cttctacgag tcgctcgacc ggatcccgca
     1561 gggcgtcgcg gtgaccatcg agttcgccgg gcccctgacg gttgcggtcc tgggctcccg
     1621 gcggttcatg gacctggtgt gggtcacgct ggccgccgcg ggcatcgtgc tgctggccaa
     1681 cctgggcggc gcgggagtcg acatgacggg cgtggccctg gccctggccg cgggcgtgtt
     1741 ctggggctcc tacatcatca tcagcgcccg ggtgggccag acgttcccgc ggatggaggg
     1801 cctggcgctg gcgatggtgt tcggggccgt ggtgctgatc ccgttcggcg tcgccggggg
     1861 cggcacggcg ctgctggacc cgcacgtgct ggccgtcggg gccggcgtgg cggtcctgtc
     1921 ctcggcgatc ccctactcgc tggagctgga ggcgctgcgg cgcctgccca ccggcacctt
     1981 cggcgtgctg atgagcctgg agcccgcggt ggcggcgctc tcgggcctgg tcgtgctcgg
     2041 ccaggtgctc gacacgcgcg agtgggccgg catcgcgctg gtggtcgcgg ccagcgcggg
     2101 ggccgccacc ttcgcaaccc gggaaacggt tgcccgggac tagtagcgtt gagcccgtga
     2161 gccacccaga cagctttggt gccaaggccg aactcgaagt cggcggtacc acccacgaga
     2221 tcttccgcct cgatgccctg cagtcggagt tcgacgtggc gcgcctgccc tactcgctga
     2281 aggtgctgct ggagaacctg ctgcgcaacg aggacggcga gtcggtcacc aaggagacca
     2341 tcgagtcggt ggccaagtgg gtggcctccg acgagccctc gatcgagatc gcctactcac
     2401 ccagccgcgt gctgatgcag gacttcaccg gcgtgccggc catcgtcgac ctggccgcca
     2461 tgcgcgacgc catggaggag ctgggcggcg acccgaccaa gatcaacccg ctggtgccgg
     2521 tcgagctggt catcgaccac tcgatccagg tggactcgtt cgccgagaag atggcgttca
     2581 agaagaacgc cgagctggag ttcgagcgca accaggagcg ctacgcgttc ctgcgctggg
     2641 gccagacggc gttcgacaac ttctccgtgg tgccgcccaa caccggcatc tgccaccagg
     2701 tgaacctgga gtacctgtcg cgcgtggtca ccgcccgcga cgg
//
LOCUS       DASUPW010000208         3428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1989567, whole genome shotgun sequence.
ACCESSION   DASUPW010000208 DASUPW010000000
VERSION     DASUPW010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1989567"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /gene="mltG"
                     /locus_tag="VFY44_04855"
     CDS             <1..1247
                     /gene="mltG"
                     /locus_tag="VFY44_04855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX5909800.1"
                     /translation="PLPPEPEPEPEPEPEPAPAVRPPTPPAPFPSARPAPRLEGETPE
                     QRRRATASALLQRRRESGTKPPRKRRGPLRVVALAVLVLVVGVVGWFVLSLFQPLKGD
                     GDGDVQVRIPTGASVGQIADLLAARDVISSPFFFQARATVSGRRDDFKAGRFTLKRGM
                     SNAAAMDEISEGPPADVVRVAVPEGRSRREVARIVGDRLRGDYIAATRRSPQLDPRRY
                     GAPRSASLEGFLFPSTYEFKKGSRTSRLVSEQVGLFKRNFARVDLAFAKRRKLNAYDI
                     LTIASMIDREASVARERPVIASVIYNRLREGIPLGIDATIRYATNNWSRPLTQSQLAI
                     DSPFNTRTRRGLPPGPIGSPGLAAIRAAAQPARTQYLFYVVKPGTCGEHAFAKTDAEF
                     QRDVERYNRERARRGGKSPTRC"
     gene            1247..2038
                     /gene="aroE"
                     /locus_tag="VFY44_04860"
     CDS             1247..2038
                     /gene="aroE"
                     /locus_tag="VFY44_04860"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX5909801.1"
                     /translation="MAPPLLGVAGFPVAHSRSPVMHAAALAELGLDWEYLKLPVPPDL
                     FAETMRALPRSGFRGVNVTVPHKEAALALADSSSPVAAAVGAANTLSFADGGAIAADN
                     TDAPGFLAALGQDARGLRATVLGAGGSARAVAWALADAGAERVAVVNRTPGRAVALAE
                     DLGVDVDTTPAAGDLLVNCTSVGLEPTEDGQALDSLGLGALEPPAVVVDLVYGDGPTP
                     VERWAAAGGARFVGGREVLVQQGALSLALWTGRPAPVDVMRAALR"
     gene            2098..2637
                     /locus_tag="VFY44_04865"
     CDS             2098..2637
                     /locus_tag="VFY44_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909802.1"
                     /translation="MSRPFVLTVLGVLLAVATFASMRSAAERAQADETADAPAVQPAP
                     GATTTTPKPAAPAKTDTTPAKKPKAAKKEPAPAVRGVPANVGKALQKRRTVVLFFRQP
                     AADDAATAEAVRSVRGIKGVSVFSAPITKLARYRRVVNDLGVTQAPAVVIVGKDRKAR
                     LLEGYIDPVSLRQQVKDGR"
     gene            2703..>3428
                     /locus_tag="VFY44_04870"
     CDS             2703..>3428
                     /locus_tag="VFY44_04870"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX5909803.1"
                     /translation="MDATGLTPPTRRTGPSRFIADVIVALGYVDRQQADAAVQESRRV
                     GKRTGQVLIDWGALSREQLAHALAERFGLDYVDLSVFQPDLGAVNLISPQAAKRFDAV
                     PIGFDESGALIVAMADPSNVLALDDLKLMTGHEVRPAVASAEDIAGVIARMSRLDEAV
                     ATALEEGEEDEELAPVTEIRESAEDAPVIKLVNSIIGQAVEEGVSDIHLEPDGRDMRV
                     RFRVDGVLKETTTIPRRMVAGVIS"
BASE COUNT          446 a         1309 c         1248 g          425 t
ORIGIN      
        1 agccgcttcc cccggagccg gaaccggagc ccgagcccga gccggagccc gcacccgctg
       61 tccggccccc gacgccgccc gctcccttcc cgagcgcgcg cccggccccg cggctggagg
      121 gcgagacccc cgagcagcgc cgccgcgcca ccgcctcggc gctgctgcag cgccggcgcg
      181 agtccgggac caagcctccc cgcaagcgcc gcgggccgct gcgggtcgtc gcgctggccg
      241 tgctcgtcct ggtggtgggc gtcgtgggct ggttcgtgct gtcgctgttc cagccgctca
      301 agggcgacgg cgacggcgac gtgcaggtgc gcatccccac gggcgccagc gtggggcaga
      361 tcgccgacct gctggctgcg cgggacgtga tctcgagccc gttcttcttc caggcccggg
      421 ccacggtgtc cggccgccgc gacgacttca aggcggggcg gttcacgctc aagcgcggca
      481 tgagcaacgc cgccgccatg gacgagatct ccgagggccc gccggccgac gtggtgcgcg
      541 tggccgtgcc cgaggggcgg tcccggcgcg aggtcgcccg gatcgtcggc gaccgcctgc
      601 gcggcgacta catcgcggcc acccgccgct caccgcagct ggacccccgc cggtacggcg
      661 ccccccgcag cgccagcctc gaggggttcc tgttcccgtc cacgtacgag ttcaagaagg
      721 gcagccgcac ctcgcggctg gtctccgagc aggtcgggct gttcaagcgc aacttcgcca
      781 gggtcgacct ggcgttcgcc aagcgccgca agctcaacgc ctacgacatc ctcacgatcg
      841 cctcgatgat cgaccgcgag gcgtcggtgg cgcgggagcg gccggtcatc gcctcggtga
      901 tctacaaccg cctgcgcgag gggatcccgc tggggatcga cgcgaccatc cgctacgcca
      961 ccaacaactg gtcgcggccg ctcacgcagt cgcagctggc catcgactcg cccttcaaca
     1021 cgcggacgcg ccgcggcctg cccccggggc cgatcgggag ccccggcctg gccgccatcc
     1081 gcgccgccgc gcagcccgcg cggacgcagt acctgttcta cgtggtcaag ccgggcacgt
     1141 gcggcgagca cgcgttcgcc aagacggacg ccgagttcca gcgcgacgtc gagcgctaca
     1201 accgcgagcg cgcgcgccgc gggggcaagt cgcccacgcg ctgctgatgg cgccgcccct
     1261 gctcggggtc gccgggttcc cggtggccca cagccgctcc ccggtcatgc acgccgcggc
     1321 gctggccgag ctgggcctgg actgggagta cctcaagctg ccggtgcccc ccgacctgtt
     1381 cgccgagacg atgcgggcgc tgccgcgctc gggcttccgc ggcgtgaacg tgaccgtgcc
     1441 ccacaaggag gcggccctgg cgctggcgga cagcagctcg ccggtggccg cggcggtcgg
     1501 cgccgccaac accctgagct tcgccgacgg tggcgcgatc gcggccgaca acaccgacgc
     1561 gccgggcttc ctggccgcgc tggggcagga cgcgcgcggc ctgcgggcga cggtgctggg
     1621 cgccggggga tcggcacgcg ccgtcgcgtg ggccctggcc gacgcgggag ccgagcgcgt
     1681 ggccgtggtc aaccgcacgc ccgggcgcgc ggtggcgctg gccgaggacc tgggcgtgga
     1741 cgtggacacc acgcccgcgg ccggtgacct gctggtcaac tgcaccagcg tcggcctcga
     1801 gcccaccgag gacggccagg cgctggactc gctgggcctg ggcgccctgg agccgcctgc
     1861 cgtggtggtg gacctggtct acggcgacgg gccgaccccg gtcgagcgct gggccgccgc
     1921 cggtggcgcg cgcttcgtgg gtggcaggga ggttctggtg cagcagggcg cgctcagcct
     1981 ggcgctctgg acggggcgcc cggcccccgt ggacgtgatg cgcgccgccc tgcgctgaaa
     2041 aaccgcgttc acgggactca accattcgtc cgggctgccg atacaccagg catggcagtg
     2101 tcccggccat tcgtgctgac cgtgctcggc gttcttctcg ccgtcgccac cttcgcctcc
     2161 atgcgctccg ccgccgagcg ggcccaggcc gacgagacgg ctgacgcgcc ggcggtgcag
     2221 cccgcgcccg gtgccaccac cacgacgccg aagccggccg ctcccgccaa gaccgacacc
     2281 acccccgcca agaagcccaa ggcggcgaag aaggagcccg cccccgcggt acgaggcgtg
     2341 ccggcgaacg tgggcaaggc gctccagaag cggcgcaccg tggtcctgtt cttccgccag
     2401 cccgccgccg acgacgcggc gaccgccgag gccgtgcgct ccgtgcgcgg catcaagggc
     2461 gtgtcggtgt tctcggcgcc catcaccaag ctggcccgct accgccgcgt ggtcaacgac
     2521 ctgggcgtga cccaggcgcc ggccgtggtc atcgtgggca aggaccgcaa ggcccgcctg
     2581 ctcgagggct acatcgatcc cgtgtccctg cgccagcagg tcaaggacgg acgatgagcg
     2641 aggccccgcc acaggggccg ggagcgccgg gcgcgggttc cgtcgcggcc ctcaagcctg
     2701 aggtggacgc caccgggctg acgccgccga cccgccggac cggcccgtcc cgcttcatcg
     2761 ccgacgtgat cgtcgcgctc gggtacgtcg atcgccagca ggcagacgcc gcggtgcagg
     2821 agtcgcgccg ggtgggcaag cgcaccggcc aggtgctgat cgactggggc gccctgtccc
     2881 gcgagcagct ggcgcacgcg ctggccgagc gcttcggcct ggactacgtg gacctgtcgg
     2941 tcttccagcc cgatctgggc gccgtgaacc tgatcagccc gcaggccgcc aagcggttcg
     3001 acgcggtgcc gatcggcttc gacgagtcgg gcgcgctgat cgtggccatg gccgacccgt
     3061 ccaacgtgct ggccctggac gacctgaagc tcatgaccgg ccacgaggtg cgccccgccg
     3121 tggcctccgc cgaggacatc gccggcgtga tcgcccggat gagccgcctg gacgaggccg
     3181 tcgccaccgc cctcgaggag ggggaggagg acgaggagct ggcgccggtc accgagatcc
     3241 gcgagtccgc cgaggacgcg ccggtcatca agctcgtgaa ctccatcatc ggccaggccg
     3301 tggaggaggg cgtgtcggac atccacctcg agcccgacgg ccgcgacatg cgcgtgcgct
     3361 tccgcgtgga cggcgtcctg aaggagacca ccacgatccc gcgccgcatg gtcgccgggg
     3421 tgatctcc
//
LOCUS       DASUPW010000209         2211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1851261, whole genome shotgun sequence.
ACCESSION   DASUPW010000209 DASUPW010000000
VERSION     DASUPW010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1851261"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VFY44_04875"
     CDS             complement(<1..807)
                     /locus_tag="VFY44_04875"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEX5909804.1"
                     /translation="MIDDGIDTAAPLMGATSEVGRLGTVLLHRPGAELRRLTPRNNDE
                     LLFDGIPWVDRAQEEHDAFAQALRDRGVEVLHLDRLLAEVLSFPSARAELIAAAVDDP
                     RLGATLQRATTTHLSGLDPEDLASALIAGLAHDELHGGKGLAFEVMDRGDFVVPPLPN
                     LLFTRDSSVWVGDEVAVTSLAMPARHRESTITAAVYAHHPRFAGVEQLYGAHLEHLEG
                     GDVLLLSPGVVAVGVGERTTPGGAERLARRLFARRLAHTVLVVPIAQQRAT"
     gene            complement(891..1340)
                     /locus_tag="VFY44_04880"
     CDS             complement(891..1340)
                     /locus_tag="VFY44_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909805.1"
                     /translation="MTQQSNWQAQAGIGPGQRGQAATGWAAWISFAAVILAVIGVLQI
                     MQGLAALIDDGFYVVRESGLVVNVDYSVWGWTHMLLGIVAILVAAGLLAGNTVARVAG
                     VILAMVSAIVNLAFLPAYPWWSTVVIVFDVLVIYALTAHGGEMKQRH"
     gene            1500..>2211
                     /locus_tag="VFY44_04885"
     CDS             1500..>2211
                     /locus_tag="VFY44_04885"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5909806.1"
                     /translation="MDLFDLTGKVAVVTGGTRGIGLMMARGLLQAGARVYISSRKAEA
                     GDAAVAELSSFGQVRSVPADLSQEEECARLAAAVGEREDEVHVLVNNAGATWGAPSLE
                     EFPSSAWDKVLDLNLKAPFFLTRAFLPLLEEAATADDPARIVNVGSIDGLHVPPMPTY
                     SYSTSKAALHHLTRVLAKELGPRHITVNAVAPGPFESKMMAATLAAAGDAIAHAIPLK
                     RIGRPDDMAGAAIFLSSRA"
BASE COUNT          300 a          816 c          817 g          278 t
ORIGIN      
        1 cgtcgcccgc tgctgggcga tcgggacgac gagcacggtg tgcgccaggc ggcgggcgaa
       61 cagccgtcgg gccagccgct ccgcgccgcc gggcgtcgtg cgctccccga ccccgaccgc
      121 gaccaccccg ggggacagca gcagcacgtc gccgccctcc aggtgctcga ggtgcgcgcc
      181 gtagagctgc tcgacgccgg cgaaccgcgg gtggtgggcg tagacggcgg cggtgatcgt
      241 gctctcgcgg tgccgggcgg gcatggccag cgacgtcacg gccacctcgt cgcccaccca
      301 gaccgaggag tcccgggtga agagcaggtt gggcagcggg gggacgacga agtcgccgcg
      361 gtccatgacc tcgaaggcca ggcccttccc gccgtgcagc tcgtcgtggg cgaggccggc
      421 gatgagcgcg ctggcgaggt cctcggggtc cagcccggac aggtgcgtcg tggtggcccg
      481 ctgcagcgtg gcgcccaggc gcgggtcgtc caccgccgcg gcgatgagct cggcgcgggc
      541 cgaggggaac gacagcacct ccgccagcag ccggtccagg tgcagcacct cgacgccgcg
      601 gtcgcgcagc gcctgggcga acgcgtcgtg ctcctcctgc gcgcggtcca cccacgggat
      661 gccgtcgaac agcagctcgt cgttgttgcg gggggtgagc cgccgcagtt cggcgcccgg
      721 gcggtgcagc aggaccgtgc ccagccggcc gacctcgctg gtggcgccca tgagcggggc
      781 ggcggtgtcg atcccgtcgt cgatcatggg cagaggctcg cacaggccgg ccgggtgcgg
      841 cgcgccacct cgccggatgc cggcgcgggg cgccggtcag cgggccgtga tcagtgacgc
      901 tgcttcatct ccccgccgtg ggcggtcagg gcgtagatga cgagcacgtc gaagacgatg
      961 accaccgtcg accaccacgg gtaggcgggc aggaaggcca ggttcacgat ggcgctcacc
     1021 atcgcgagga tcacgccggc cacgcgcgcc acggtgttgc cggcgagcag gcccgccgcg
     1081 acgaggatcg ccacgatccc gaggagcatg tgcgtccagc cccacaccga gtagtcgacg
     1141 ttgacgacca gcccgctctc ccgcaccacg tagaagccgt cgtcgatgag cgcggccagg
     1201 ccctgcatga tctgcagcac cccgatgacg gccaggatca ccgcggcgaa cgagatccag
     1261 gcggcccagc cggtcgccgc ctgacctcgc tgcccggggc cgatccccgc ctgtgcctgc
     1321 cagttcgact gctgggtcat cgacctctcc ctccggtggc aacgtcctcc ccggatgctg
     1381 tcgccgcgcc cgggcgaccg cctcatccgg aacaggtgag ccgggcccgg ccgggcggct
     1441 ggctagggtg cccgggaggc cggcggcgag gccggcccca cccgatccgg aggcagtccg
     1501 tggacctgtt cgacctgacc ggcaaggtgg ccgtcgtcac cggtggcacc cgcggcatcg
     1561 gcctgatgat ggcccgcggc ctcctgcagg cgggggcccg cgtctacatc agctcccgca
     1621 aggccgaggc cggtgacgcc gccgtcgcgg agctctcctc cttcggccag gtccgctccg
     1681 tccccgccga cctgtcgcag gaggaggagt gcgcccggct ggccgcggcc gtcggcgagc
     1741 gcgaggacga ggtgcacgtc ctggtcaaca acgccggggc gacgtggggc gcgccgtcgc
     1801 tggaggagtt cccgtcatcg gcctgggaca aggtgctcga cctgaatctg aaggcgccgt
     1861 tcttcctgac gcgggccttc ctgcccctgc tcgaggaggc ggccaccgcc gacgaccccg
     1921 cgcgcatcgt gaacgtcggc agcatcgacg gactgcacgt gccgcccatg ccgacctact
     1981 cgtactcgac gagcaaggcg gcgctgcacc acctcacgcg ggtgctggcc aaggagctgg
     2041 ggccgcggca catcacggtc aacgccgtcg ccccggggcc gttcgagtcc aagatgatgg
     2101 cggcgacgct ggcggcggcg ggcgacgcca tcgcccacgc catcccgctc aagcgcatcg
     2161 ggcgccccga cgacatggcc ggcgcggcca tcttcctgtc gtcgcgtgcg g
//
LOCUS       DASUPW010000210         2914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1435641, whole genome shotgun sequence.
ACCESSION   DASUPW010000210 DASUPW010000000
VERSION     DASUPW010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435641"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VFY44_04890"
     CDS             <1..573
                     /locus_tag="VFY44_04890"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX5909807.1"
                     /translation="LGVFVLMTLESACIPVPSEATMLFAGFNVSDGEYSLFAAVAVGS
                     VANLVGSWIAYGIGYYGRIDILEKHGKKLHIKKSHLELADRWFERHGDATVFFTRMLP
                     IIRTFISLPAGVAKMPFWRFSVLTLAGCIPWVLMLTYIGKEAGDNWEKWKDSLHYVDY
                     AVAAAIVIGVAYLVIRRWRRPPPDAAVESA"
     gene            638..2347
                     /locus_tag="VFY44_04895"
     CDS             638..2347
                     /locus_tag="VFY44_04895"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX5909808.1"
                     /translation="MSSQHASPSAAGAPSRFTRAPERSSAEPLDLVADHLRRHPRIDG
                     GAFQTVDQGRRLVRPAVSWFASPVLAEIVSQVLERPYARSSPGFTEATLERGRPLFLP
                     RVEDWEAAPSLRQLLDRAGPAAGAELWTALARASVIACPVRDSLGRIVGTLEAASFDP
                     ARPLTREDLGDVAVIADLAALAYERSSFLAQETARNRIELVLKRAAADITGSLETEEV
                     LRHVAEHALPAVNADRAVVCRMLPSGELSRAAAAPTEGGPVIDAGQVAQVAQARAAAR
                     FEGDVASAHVPLVLGPRLFGVLSVAREHPFSEDETEILLRLARTSVGAIVNAAHFEQE
                     RGVARALTRGFVPDSPPSMDEYETAALYQPAGGESAGGDVYGLWKRPGGDLALLIGDV
                     AGKGAAAAGTSAMTRFFVEARSWDADGPGQILAQTGRMLHERLPADTFVTAFMAVLRD
                     GELRYANAGHLPPLLMRADGLTAPVQAMGLPLGIDPEAEYEEQTLILESGDLLYAYTD
                     GLTEARRHGEMLGEGRLREALDAQRGAAGSVDDLVRAVHEAVRGWAGILVDDSTALAV
                     RQR"
     gene            2363..>2914
                     /locus_tag="VFY44_04900"
     CDS             2363..>2914
                     /locus_tag="VFY44_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEX5909809.1"
                     /translation="MGDSAYARAGVDTGQAGAGVAALVGVLKTIDTGRESRALLGSGH
                     YANVLRLTGDSGLALSTDGVGTKLIVAEQMGRYDTVGIDCIAMNVNDVICVGADPIAV
                     LDYVAIEQADPALLEAVGVGLKAGAEDAGVEIPGGELAVVPELIRGHPSPGGIDLVGM
                     CVGLVDIPRIVTGASVAPGDAIVG"
BASE COUNT          368 a         1054 c         1083 g          409 t
ORIGIN      
        1 ctcggcgtct tcgtgctgat gaccctggag tcggcctgca tccccgttcc cagcgaggcc
       61 acgatgctgt tcgccggctt caacgtgtcc gacggcgagt actcgctgtt cgccgccgtg
      121 gccgtcggct cggtggccaa cctggtcgga tcctggatcg cgtacgggat cggctactac
      181 ggccggatcg acatcctgga gaagcacggc aagaagctgc acatcaagaa gagccacctg
      241 gagctggccg accgctggtt cgagcgccac ggcgacgcca ccgtgttctt cacccggatg
      301 ctgcccatca tccgcacctt catctcgctg ccggcgggcg tggcgaagat gccgttctgg
      361 cgcttctcgg tgctcacgct ggccggctgc atcccctggg tcctgatgct gacctacatc
      421 ggcaaggagg ccggggacaa ctgggagaag tggaaggact cgctgcacta cgtggactac
      481 gcggtggccg cggccatcgt gatcggcgtg gcctatctgg tcatccgccg ctggcgccgg
      541 ccgccgccgg atgccgccgt cgagagcgcc tgatacgggc gcgttcgcgg gttagcgcgc
      601 tcgcgggatc ggacctatct tgggaccgat cgccccgatg agctcccagc acgcgtcccc
      661 atccgcggcc ggagcaccgt cgcgcttcac gcgcgcgccg gaacgctcca gcgccgagcc
      721 cctggacctg gtggcggacc acctgcgccg tcatccgcgg atcgacggtg gcgccttcca
      781 gaccgtggac cagggccggc ggctcgtgcg cccggcggtc tcgtggttcg cgtcgccggt
      841 gctggccgag atcgtttccc aggtgctcga acgcccctac gcgcgctcca gccccgggtt
      901 caccgaggcg acgctggaac ggggccggcc gctgttcctg ccccgggtcg aggactggga
      961 ggccgcgccg tcgctgcgcc agctgctcga ccgggccggc cccgccgccg gcgccgagct
     1021 gtggaccgcg ctggcgcgcg catccgtgat cgcctgcccg gtgcgggact cgctgggcag
     1081 gatcgtcggc acgctcgagg cggcctcgtt cgaccccgcc cgcccgctga cgcgcgagga
     1141 cctgggcgac gtcgcggtca tcgccgacct ggcggcgctg gcctacgagc gctccagctt
     1201 cctggcccag gagacggcgc gcaaccggat cgagctggtg ctcaagcgcg ccgccgccga
     1261 catcaccggc tccctggaga ccgaggaggt gctgcggcac gtggccgagc acgcgttgcc
     1321 cgcggtgaac gccgaccgcg ccgtggtctg ccggatgctg cccagcggtg agctgtcccg
     1381 ggcggccgcc gcgccgaccg agggcgggcc ggtcatcgac gccggccagg tggcgcaggt
     1441 cgcgcaggcc cgcgcggccg cgcggttcga gggcgacgtg gcctcggcgc acgtcccgct
     1501 ggtgctgggg ccgcgcctgt tcggggtgct gtcagtggcc cgggagcacc cgttctccga
     1561 ggacgagacc gagatactgc tgcgcctggc ccggacctcg gtcggcgcca tcgtcaacgc
     1621 ggcccacttc gagcaggagc gcggggtggc gcgggcgctc acgcgcgggt tcgtccccga
     1681 ctcgccgccc tcgatggacg agtacgagac cgccgcgctg taccagccgg ccggcggcga
     1741 gagcgccggt ggcgacgtgt acggcctgtg gaagcgcccc ggtggggacc tggcgctgct
     1801 gatcggcgac gtggccggca agggcgcggc ggccgcgggc accagcgcca tgacgcgctt
     1861 cttcgtggag gcgcgcagct gggacgcgga cgggcccggc cagatcctgg cccagaccgg
     1921 caggatgctg cacgagcgcc tgcccgccga caccttcgtg accgcgttca tggccgtgct
     1981 gcgcgacggc gagctgcgct acgcgaacgc cggccacctg ccgccgctgc tcatgcgggc
     2041 cgacggcctg accgcccccg tgcaggccat gggcctgccg ctgggcatcg acccggaggc
     2101 cgagtacgag gagcagacgc tgatcctgga atccggcgac ctgctctacg cctacacgga
     2161 cggcctgacc gaggcccgcc gccacggcga gatgctcggc gagggccgtc tgcgcgaggc
     2221 cctggacgcc cagcgcggcg ccgccggctc ggtggacgac ctggtgcggg ccgtgcacga
     2281 ggccgtgcgg ggctgggccg ggatcctggt cgacgactcc acggcactgg ccgtacgcca
     2341 gcgctgatta actcccccgc cgatgggtga ctcggcatac gcacgcgccg gcgtggacac
     2401 cggccaggcc ggcgccggcg tggccgccct ggtgggggtc ctcaagacga tcgacaccgg
     2461 ccgcgagagc cgggcgctgc tgggctcggg ccattacgcg aatgtcctgc ggctgacggg
     2521 cgactccggg ctggcgctgt ccaccgacgg ggtgggcacc aagctcatcg tggccgagca
     2581 gatgggccgc tacgacaccg tcggcatcga ctgcatcgcc atgaacgtga acgacgtgat
     2641 ctgcgtgggc gccgacccga tcgcggtgct ggactacgtg gccatcgagc aggccgaccc
     2701 cgcgctgctc gaggcggttg gcgtgggcct caaggcgggc gccgaggacg cgggcgtgga
     2761 gatcccgggc ggcgagctgg ccgtggtgcc cgagctgatc cgcggtcacc cctccccggg
     2821 cggcatcgac ctggtgggca tgtgcgtggg cctcgtggac atcccgagga tcgtgaccgg
     2881 cgcctcggtg gcccccggcg acgccatcgt gggc
//
LOCUS       DASUPW010000211         2191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_50496, whole genome shotgun sequence.
ACCESSION   DASUPW010000211 DASUPW010000000
VERSION     DASUPW010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50496"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VFY44_04905"
     CDS             complement(<1..519)
                     /locus_tag="VFY44_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5909810.1"
                     /translation="MFKEEAAWIRWILGRQDLPEGGTVADIGSSTLFARAVGQPYVEG
                     QVLQPLRDRGLSITHVDAVEDPGVDVVADLADPATDLPGLIGGQADLVLCLSVLAFVR
                     DLPPALAHVASAVKPGGLIVATTPRRYRVTRNPVDNGWRPTPTELLDRLQADGVRFER
                     IAAAELVIHDNEE"
     gene            complement(530..1741)
                     /locus_tag="VFY44_04910"
     CDS             complement(530..1741)
                     /locus_tag="VFY44_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5909811.1"
                     /translation="MAVAVPAADMTDEQKAITDMVRQFADEQILPNAEHYDHEDEFPE
                     PIVEQMKELGLFGVTIPEEYGGMGLDLTTYVMIVEELSRGWISISGIINTHFIGSYLL
                     MKYGNDEQKQNYLPRMATGEIRAAFSLSEPELGSDVQAIKTQAKKLDDGNYEINGQKM
                     WVTNGLRSTLVFTLVKTDPDAEKRHKGMTCFIAEKEAGVHENEGEYAGFNVPPQLKKM
                     GYKGVESTELVFDGYKCRAENILGGEEAGLNKGFAQMMDALEVGRVNVAARGVGIAKR
                     ALELALRYSQERKTFGVQIADHQAIQFKLADMATQAEAAQLLTRQAARLKDAGERSDL
                     EAGMAKLFASEAGRFCVEESFRIHGGYGYSKEYEIERLYRDAPLLLIGEGTSEIQRMV
                     IGKKLLQRHKI"
     gene            1879..>2191
                     /locus_tag="VFY44_04915"
     CDS             1879..>2191
                     /locus_tag="VFY44_04915"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX5909812.1"
                     /translation="MRTVVLAATLLLATAACADAARPDPNYPAGFSERVLVDGLQAPT
                     AVAWTPDGRMLIVEKGGVVKVATRASADATEILDISGRVNAAGDRGLLGVAVDANYGS
                     GA"
BASE COUNT          299 a          752 c          762 g          378 t
ORIGIN      
        1 ctcctcgttg tcgtggatca ccagctccgc ggcggcgatc cgctcgaagc gcaccccgtc
       61 cgcctggagc cgatccagga gctcggtcgg cgtcggccgc cagccgttgt ccaccgggtt
      121 gcgggtgacg cggtaccggc gcggcgtggt ggccacgatc aggcccccgg gcttgaccgc
      181 cgaggccacg tgggccagcg cgggtggcag gtcgcgcacg aaggccagca cgctcaggca
      241 cagcaccagg tcggcctggc cgccgatcag gccgggaagg tcggtggccg ggtcggccag
      301 gtcggccacc acgtccacgc ccgggtcctc gaccgcgtcc acgtgggtta tcgacaggcc
      361 gcgatcgcgc agcggctgca gcacctggcc ctccacgtag ggctggccga ccgcgcgcgc
      421 gaacagcgtc gaggagccga tgtcggccac ggtgccgccc tcgggcaggt cctgcctgcc
      481 caggatccac ctgatccagg cggcttcctc cttgaacatg cccgggcgcc tagatcttgt
      541 gccgctgcag gagcttcttg ccgatcacca tgcgctggat ctcggacgtg ccctcgccga
      601 tcaacagcag cggcgcgtcg cggtagaggc gctcgatctc gtactccttg gagtagccgt
      661 agccgccgtg gatgcggaac gactcctcga cgcagaagcg gcccgcctcg gaggcgaaca
      721 gcttggccat gccggcctcc aggtcggagc gctcgcccgc gtccttgagg cgggcggcct
      781 ggcgggtcag cagctgcgcg gcctcggcct gggtggccat gtcggccagc ttgaactgga
      841 tggcctggtg gtcggcgatc tggacgccga acgtcttgcg ctcctgggag tagcgcaggg
      901 ccagctccag ggcgcgcttg gcgatgccca cgccgcgggc cgccacgttc acgcggccga
      961 cctcgagcgc gtccatcatc tgcgcgaagc ccttgttcag gcccgcttcc tcgccgccga
     1021 ggatgttctc ggcgcggcac ttgtagccgt cgaagaccag ctcggtggac tccacgccct
     1081 tgtagcccat cttcttgagc tgcggcggca cgttgaagcc ggcgtactcg ccctcgttct
     1141 cgtggacgcc cgcctccttc tcggcgatga agcaggtcat gcccttgtgg cgcttctcgg
     1201 cgtccgggtc ggtcttgacc agggtgaaca ccagcgtgga gcgcaggccg ttggtgaccc
     1261 acatcttctg gccgttgatc tcgtagttgc cgtcgtccag cttcttggcc tgcgtcttga
     1321 tggcctggac gtcggagccc agctcgggct cggacagcga gaacgcggcc cggatctcac
     1381 cggtggccat gcgcggcagg tagttctgct tctgctcgtc gttgccgtac ttcatgagca
     1441 ggtaggagcc gatgaagtgg gtgttgatga tcccggagat cgagatccag ccccgcgaga
     1501 gctcctcgac gatcatcacg taggtcgtca ggtccaggcc catcccgccg tactcctcgg
     1561 ggatggtcac cccgaacagg ccgagctcct tcatctgctc cacgatcggc tcggggaact
     1621 cgtcctcgtg gtcgtagtgc tcggcgttcg ggaggatctg ctcgtccgcg aactggcgca
     1681 ccatgtcggt gatcgccttc tgctcgtcgg tcatgtcggc ggcggggaca gctacggcca
     1741 tgggattcgg cgctccgggg ttgcttacgg ggccagtaag aggggtccgg aggatacctg
     1801 ctggcggatc agcggaacct gacctacctt ggccgagtgt cggggtacca gtatgggggc
     1861 accgctctac tgagagcggt gcgaacggtc gtgctcgcag caacgctgct gctggccacg
     1921 gctgcgtgcg cggacgccgc gcgaccggat cccaactacc ccgccggctt ctccgagcgg
     1981 gtgctggtcg acggcctcca ggcccccacc gcggtggcct ggaccccgga cggacggatg
     2041 ttgatcgtcg agaagggcgg cgtggtcaag gtggccaccc gggcctccgc cgacgccacc
     2101 gagatcctgg acatctccgg ccgggtgaat gccgccggcg accgcgggct gctcggcgtc
     2161 gcagtggacg ccaactacgg ctcgggcgcc g
//
LOCUS       DASUPW010000212         2430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_50849, whole genome shotgun sequence.
ACCESSION   DASUPW010000212 DASUPW010000000
VERSION     DASUPW010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50849"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /gene="wecB"
                     /locus_tag="VFY44_04920"
     CDS             complement(<1..1030)
                     /gene="wecB"
                     /locus_tag="VFY44_04920"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX5909813.1"
                     /translation="MTVIGNRPQFVKAAAVSRLLRERHEEVLVHTGQHYDDELSRVFF
                     DELGVPAPDRQLGAGGGSNTEQTARILGLLEGVIQAEAPGMVMVYGDTNSTLAAALAA
                     AQARVPIAHVEAGMRSFDRAMPEELNRVLSDHASDLLLCSTQTAMDNLEREHVPGERH
                     LVGDVMADVSLTFGPIARERSRALAEHGLEAGEYVLATAHRAGNVDDPARLERLVSLL
                     EAVPGRVVLPLHPRTAARLQEFGLRGRLDAAEGVQLLPPLGYLDFMRLAMDARAVLTD
                     SGGVQKEAYLLGVRCVTLRETTEWVETGDAGWNTLVGLDRAAAVAALEAPAPAGERPE
                     LYGGGKAAG"
     gene            1050..1172
                     /locus_tag="VFY44_04925"
     CDS             1050..1172
                     /locus_tag="VFY44_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909814.1"
                     /translation="MKKLLGGAVGVLTHAAWWVRYQVDGTARDDPSDAPAADDD"
     gene            complement(1169..1969)
                     /locus_tag="VFY44_04930"
     CDS             complement(1169..1969)
                     /locus_tag="VFY44_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX5909815.1"
                     /translation="MSDELAMLDGVIAPSAETVLPVTDQGFIRGDGAFEVVRVYGGSA
                     FLMDEHLDRIERSAANLRLDGPVERELIHGEVTELLERRAGDQPFDGALRLILTRGGR
                     RLLMTEPLPELPERLRLATVTYAPTRVLDGVKSLSYAANMLCSRLARERGFDDAVLVT
                     PHGRVLELPTASLFWVGDDGVLCTTPLSEHVLASITRAHLMRLLEVEERVITAEQLLE
                     VREAFVASTAREVHPVAAIEDRQFEQVGEVTRKAAGAFSAHIDELLGR"
     gene            complement(1982..>2430)
                     /locus_tag="VFY44_04935"
     CDS             complement(1982..>2430)
                     /locus_tag="VFY44_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5909816.1"
                     /translation="PMVYNYFGSKEGIYLAYLARAGRDLLNRMLAADVPGGSLQERLT
                     AGAGAFFGFVGENHDGWRVLREEMVQQREAVGGQFATMRDAIHALLSEVLLEEGGDPA
                     RVDAFARALTGAGESLADWWLEHPDTPVEVLVETLVAMAGAAAPAR"
BASE COUNT          326 a          921 c          869 g          314 t
ORIGIN      
        1 ggcccgcggc cttgccgccg ccgtagagct ccgggcgctc cccggccggg gccggggcct
       61 cgagcgcggc cacggcggcc gcgcggtcca gcccgaccag cgtgttccag cccgcgtccc
      121 cggtctcgac ccactcggtg gtctcgcgca gcgtcacgca gcgcacgcct agcaggtagg
      181 cctccttctg gaccccgccc gagtcggtca ggacggcgcg ggcgtccatg gcgaggcgca
      241 tgaagtccag gtagcccagc ggcggcagca gctgaacccc ctcggccgcg tccaggcggc
      301 cgcgcaggcc gaactcctgg aggcgcgcgg ccgtccgcgg gtgcagcggc agcacgacgc
      361 gccccggcac cgcctccagc agcgacacca gccgctcgag gcgcgccggg tcgtccacgt
      421 tgcccgcccg gtgcgcggtg gccagcacgt actccccggc ctccaggccg tgctcggcca
      481 gggcgcgtga gcgctcgcgc gcgatcgggc cgaacgtcag cgacacgtcg gccatgacgt
      541 caccgaccag gtggcgctcg ccgggcacgt gctcgcgctc gaggttgtcc atcgcagtct
      601 gcgtcgagca cagcagcagg tcgctggcgt ggtcgctcag gacccggttg agctcctcgg
      661 gcatcgcccg gtcgaaggag cgcatgcccg cctccacgtg ggcgatcggc acgcgcgcct
      721 gcgcggccgc gagggctgcg gccagcgtgg agttggtgtc tccgtacacc atcaccatcc
      781 cgggcgcctc ggcctggatc acgccctcca gcaggcccag gatccgggcg gtctgctcgg
      841 tgttcgaccc accgccggcg cccagctggc ggtccggcgc gggcaccccg agctcgtcga
      901 agaagacccg cgagagctcg tcgtcgtagt gctggccggt gtgcacgagc acctcctcgt
      961 gccgctcgcg caggagccgg gagacggccg ccgccttgac gaactgcggt cggttcccga
     1021 tgacggtcaa gatacgcatg gtgggtgcga tgaagaagct attgggcgga gccgtcggcg
     1081 tgctgaccca cgcggcgtgg tgggtgcgct accaggtgga cggcacggcc cgtgacgacc
     1141 cctccgacgc gcccgcggcg gacgacgact agcggcccag cagctcgtcg atgtgggcgc
     1201 tgaaggcgcc cgcggccttg cgcgtgacct cgccgacctg ctcgaactgg cggtcctcga
     1261 tcgccgcgac cgggtgcacc tcgcgcgcgg tcgacgccac gaacgcctcg cgcacctcca
     1321 gcagctgctc ggcggtgatc acgcgctcct cgacctcgag caggcgcatc aggtgcgccc
     1381 gcgtgatcga ggccaggacg tgctcggaga ggggcgtggt gcacaggacg ccgtcgtcgc
     1441 cgacccagaa cagcgaggcg gtgggcagct ccagcacgcg gccgtgcggc gtgaccagca
     1501 ccgcgtcgtc gaagccgcgt tcgcgggcca ggcggctgca gagcatgttg gcggcgtagg
     1561 agagcgactt cacgccgtcc agcacgcgcg tgggtgcgta ggtcacggtg gccaggcgca
     1621 ggcgctcggg caactcgggc agcggctcgg tcatcagcag ccgccggccc ccgcgcgtga
     1681 ggatcaggcg cagcgcgccg tcgaagggct ggtcacccgc gcgccgctcc agcagctccg
     1741 tgacctcgcc gtggatcagc tcgcgctcca cggggccgtc caggcgcagg ttggcggccg
     1801 agcgctcgat ccggtccagg tgctcgtcca tcaggaacgc cgagcccccg tagacgcgca
     1861 cgacctcgaa ggcgccgtcg ccccggatga acccctggtc ggtgaccggg aggaccgtct
     1921 cggccgacgg cgcgatcacg ccgtccagca tcgccagctc gtcgctcatg ggtccttctt
     1981 atcagcgcgc cggggccgct gcgcccgcca tcgcgaccag cgtctccacc agcacctcca
     2041 cgggcgtgtc ggggtgctcg agccaccagt cggccagcga ctcgcccgcc ccggtcagcg
     2101 cgcgcgcgaa cgcgtccacc cgggcggggt ctcccccctc ctcgagcagg acctccgaga
     2161 gcagcgcgtg gatcgcgtcg cgcatggtgg cgaactggcc gccgacggcc tcacgctgct
     2221 ggaccatctc ctcgcgcagc acccgccagc cgtcgtggtt ctcgcccacg aagccgaaga
     2281 aggcgccggc accggcggtc aggcgctcct gcagcgaccc gccgggcacg tcggccgcga
     2341 gcatccggtt cagcaggtcc cggcccgccc gcgccaggta ggccaggtag atgccctctt
     2401 tcgagccgaa gtagttgtag accatcggct
//
LOCUS       DASUPW010000213         2235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_50936, whole genome shotgun sequence.
ACCESSION   DASUPW010000213 DASUPW010000000
VERSION     DASUPW010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50936"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VFY44_04940"
     CDS             <1..537
                     /locus_tag="VFY44_04940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX5909817.1"
                     /translation="PATMVWVHCVEVHSFLAAYRAYARPISRAEQDRYLAEQVEAAVL
                     LGTPRHRVPDSVDAYRDYFASVRHELCTSPEAEAAIGFVRRPSLPGLPPAQRVLLQPA
                     FRAMGDAATSLVPSSLRELAGLPARGPRELPSRVAVSAASNAVAVGLRIPVLRYAVEE
                     PLTRTLGTKRRPRVPAAA"
     gene            576..>2235
                     /gene="ggt"
                     /locus_tag="VFY44_04945"
     CDS             576..>2235
                     /gene="ggt"
                     /locus_tag="VFY44_04945"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5909818.1"
                     /translation="MRRLPFAIALIVIAAAAFVAGVSVAHAKPGSKLRPPASGERGVV
                     ATESPAAARVGRSVLEGGGNAVDAAAATVFALNVARPQSCGIGGGGFMVYRSAKGTPA
                     ALDFRETAPARFSPNTLTPDGLHKDFTGHLTVGVPGTVAGLQAALHRYGTRSLAQSIR
                     PAERLAARGFAVPRSLSEAMEANADRLRLFPTAAKQFLRDGKPYPAGSVLRQPELADT
                     LRTLRKEGPKAFYRGSIARKIDAEMDRTRAKPIPGDSALLTKGDLRRYEAKWREPLVG
                     TFRGRKLVAMPPPTSGGIAVLEMLNILENYDNKASGQSSAETLHRIIEAQKLAFADRG
                     EYVADPDFVRQPTRTLTSKQYAARRAALIDPAKANPEPKPGLGPLATVANGAENPAGT
                     TTHLSVIDAWGNSVALTCTIEQEFGSAVVAPGTGFLLNNEMTDFGKPGSANEARAFKR
                     PRSSMAPIIASEAGRPVNVTGGAGGSRIIMGSLFSVFNRIEYGLPLNQAVDAERVDAN
                     EFPDKGIKVEDARLAPGVLDSLRARGHQLDLLGEYDTRPRVQAAG"
BASE COUNT          287 a          886 c          780 g          282 t
ORIGIN      
        1 ccggccacga tggtgtgggt ccactgcgtg gaggtgcact cgttcctggc cgcctaccgc
       61 gcctacgcgc gccccatctc ccgcgccgag caggaccgtt acctggccga gcaggtggag
      121 gcggcggtgc tgctgggcac cccccgccac cgcgtgcccg actcggtcga cgcctaccgc
      181 gactacttcg cctccgtgcg ccacgagctg tgcacgtcgc ccgaggccga ggccgcgatc
      241 ggcttcgtgc gccggccctc gctgcccggc ctgcctcccg cccagcgcgt gctgctccag
      301 cccgcgttcc gcgccatggg cgacgcggcc acgtcgctgg tgccgtcgtc gctgcgtgag
      361 ctcgccgggc tgcccgcccg cggcccgcgg gagctgccca gccgggtcgc ggtgtccgcg
      421 gcgtcgaacg cggtggccgt cgggctgcgg atcccggtgc tgcgctacgc cgtcgaggag
      481 cccctcacgc gcaccctggg caccaagcgc cgcccgcgcg tgcccgcggc cgcctgaccg
      541 tttcaccgcc cggccggagg ggtagccgta agggcgtgcg ccgccttccc ttcgccatcg
      601 ccctgatcgt catcgccgcc gcggccttcg tggccggcgt gtccgtcgcc cacgccaagc
      661 cgggctcgaa gctgcgcccg cccgccagcg gcgagcgcgg cgtggtggcc accgagtcgc
      721 cggcggcggc ccgcgtcggc cgctccgtcc tcgagggtgg cggcaacgcc gtcgacgccg
      781 ccgcggccac cgtcttcgcc ctgaacgtgg cccggccgca gtcgtgcggc atcggcggcg
      841 gcggcttcat ggtctaccgc tcggccaagg gcacgcccgc cgcgctggac ttccgcgaga
      901 ccgcgcccgc gcgcttctcg cccaacacgc tgacccccga cgggctgcac aaggacttca
      961 ccggacacct gaccgtgggg gtgccgggca cggtggccgg gctgcaggcg gcgctgcacc
     1021 gctacggcac gcgcagcctg gcgcagtcga tccggcccgc cgagcggctg gccgcgcgcg
     1081 gcttcgccgt gccccggtcg ctgtccgagg ccatggaggc caacgccgac cggctcaggc
     1141 tcttccccac cgcggcgaag cagttcctgc gcgacggcaa gccctacccg gccgggtcgg
     1201 tcctgcgcca gcccgagctg gccgacacgc tgcgcacgct ccgcaaggag gggccgaagg
     1261 cgttctaccg gggctcgatc gcccgcaaga tcgacgccga gatggaccgc acgcgcgcca
     1321 agccgatccc cggcgactcc gctctgctga ccaagggcga cctgcggcgc tacgaggcca
     1381 agtggcgcga gccgctggtg ggcaccttcc gcggccgcaa gctggtggcc atgcccccgc
     1441 ccacctccgg cggcatcgcc gtcctggaga tgctgaacat cctcgagaac tacgacaaca
     1501 aggcgtcggg acagagctcg gccgagacgc tgcaccgcat catcgaggcg cagaagctgg
     1561 ccttcgccga ccgcggcgag tacgtggccg accccgactt cgtgcgccag cccacgcgaa
     1621 cgctgacctc gaagcagtac gccgcccggc gcgccgccct gatcgacccg gccaaggcca
     1681 accccgagcc caagcccggc ctgggccccc tggccacggt ggccaacggg gccgagaacc
     1741 ccgccggaac gaccacccac ctgtcggtca tcgacgcctg gggcaactcg gtcgcgctga
     1801 cctgcaccat cgagcaggag ttcggctccg ccgtggtcgc gccgggcacc ggcttcctgc
     1861 tgaacaacga gatgaccgac ttcggcaagc ccggctccgc gaacgaggcg cgcgcgttca
     1921 agcgcccgcg ctcgtcgatg gcgcccatca tcgccagcga ggcggggcgc ccggtcaacg
     1981 tgaccggtgg cgccggcggc tcgcggatca tcatgggctc gctgttctcg gtgttcaacc
     2041 ggatcgagta cgggctgccg ctgaaccagg cggtcgacgc cgagcgcgtg gacgccaacg
     2101 agttccccga caaggggatc aaggtcgagg acgcgcggct ggcgcccggg gtcctggact
     2161 ctctgcgggc acgcggtcac cagctcgacc tgctgggcga gtacgacacg cgcccgcggg
     2221 tgcaggccgc gggct
//
LOCUS       DASUPW010000214         3881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1021024, whole genome shotgun sequence.
ACCESSION   DASUPW010000214 DASUPW010000000
VERSION     DASUPW010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021024"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VFY44_04950"
     CDS             <1..308
                     /locus_tag="VFY44_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909819.1"
                     /translation="VGRRLEDEPATKRATTTVRRAVGAALPPRTRKRITQRLNMDHPP
                     REKWERLQRHYAFWQQKWGWDMLNPDMDAVLERWGGTEVCWRYDDALRAAGEEILAR"
     gene            305..1288
                     /locus_tag="VFY44_04955"
     CDS             305..1288
                     /locus_tag="VFY44_04955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5909820.1"
                     /translation="MKVGVPIISTNEGELLRHTLPLVLAQEDVEVVVFDNASDDSTAE
                     VAAELGVRYVRFEERHSFGRAMNAAVRTMDTEAVLFVQPDCFVSPGMVAAAAAHLDDP
                     TVGSVAPKLIRTEGPGPEQRLDVLDTAGMSLDRRRKNGLVGHGTPSLGFDTVAEAFGA
                     DGAAALYRRAALEDAAVDGHVFDEDLVMFRDGIPADWASDADLAWRVRLMGWRCVYEP
                     GAVAHHIRRYSPSTRGRVPEWQRMVQFRNRYLMMVKNDPLPALARDLPRILLYEALAL
                     GFVLLRERFLLRGYLEAARAFPRMRAKRRVLQRRRRAREAPAPPYGLLPPA"
     gene            1306..2076
                     /locus_tag="VFY44_04960"
     CDS             1306..2076
                     /locus_tag="VFY44_04960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEX5909821.1"
                     /translation="MEAGSAPADDTTALFDIRIQTAPPAEMLRAIVGYARDGERRRVS
                     YVNAHVLNQSFADPELRRALRESDLVYCDGYGVRLAARMIGLPVPHRMTGADWVWTVA
                     AMCQEEGLSLYLLGSEPGASADAAAALRRWYPELKVHGTHHGYFGLGTPHAERVIEHL
                     AETKPDILLVGMGTPQQELWVEQNQDRIEASVVWTVGALFDYVSDRVPRAPRWIADNG
                     FEWIFRLAVEPRRMWRRYLLGNPAFLYRILEERRRPGR"
     gene            2130..>3881
                     /locus_tag="VFY44_04965"
     CDS             2130..>3881
                     /locus_tag="VFY44_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909822.1"
                     /translation="MPARAAAVIAAVLALAALSIAVYAGVKGVAGVPDAARGVLATLL
                     LFGVTGYAPAVLLLPRALRPHLPFFALGLGAAVSCLGLSVLGFFGAPLVASLVVLLGG
                     GLVAGLLLRVRRGPARADDADMAAAGGRTVAWAWPAYIALILVALCLIPTFRAGYGSV
                     PGTNPDGMLSVGTAEFLQHNPPRAVDPGLPVDQMPPVWRSKYPIYYGLAGAATLSGLD
                     TVAAYTGFAAVVTALAALGFMLLARYALRAPPVVALVALALVAFARVVPYLAIHPYFN
                     QLWGTLALGFMLLFGLRFLDDPNRRDAIAALLFVALGLSAYPLMVVFPALAFVAAAVS
                     LRRRGHGGWIRGLGLPRGRRSLLLWVPLGILVVPAALIVTLGVLEKSASATNLMLGRE
                     DLKAWRGDLTSFPGPDFYLGPPGVTGYVVAAAVLVVAFLGLRRAPATARAALGAAIAG
                     GLVLGLFFRLRTYGEYFDLKVLSFLTPLLLVATVPWLAERLSGPRTAARYAVMGAVAL
                     VLALQVAGLREELGRTALQVDRETFVLRDVARGLPPGSIRVDVPPSGRHLWASYFLNE
                     RRLSSYTPVLFTTYPHVP"
BASE COUNT          427 a         1461 c         1422 g          571 t
ORIGIN      
        1 acgtgggccg gcgcctggag gacgagccgg ccaccaagcg cgcgaccacc accgtccgtc
       61 gcgccgtggg cgccgcgctg ccaccacgca cccgcaagcg gatcacccag cggctgaaca
      121 tggaccaccc gcccagggag aagtgggagc ggcttcagcg ccactacgcc ttctggcagc
      181 agaagtgggg ctgggacatg ctcaaccccg acatggacgc cgtgctggag cgctggggcg
      241 ggaccgaggt gtgctggcgc tacgacgacg ccctgcgcgc cgcgggcgag gagatcctgg
      301 ccaggtgaag gtcggcgtcc ccatcatctc gaccaacgag ggcgagctgc tgcgtcacac
      361 cctcccgctg gtgctggccc aggaggacgt cgaggtcgtg gtgttcgaca acgcctcgga
      421 cgacagcacc gccgaggtgg ccgccgagct gggcgtgcgc tacgtgcgct tcgaggagcg
      481 gcactcgttc ggccgggcca tgaacgcagc cgtgcggacc atggacaccg aggccgtgct
      541 gttcgtgcaa ccggactgct tcgtgtcacc gggcatggtg gccgcggcgg cggcccacct
      601 ggacgacccg accgtgggct cggtcgcacc gaagctcata cggaccgagg gcccggggcc
      661 cgagcagcgc ctggacgtgc tggacaccgc gggcatgtcg ctggaccgca ggcgcaagaa
      721 cggcctggtc gggcacggca ccccctcgct gggcttcgac accgtggccg aggcgttcgg
      781 cgccgacggc gccgcggcgc tgtaccggcg cgcggcgctg gaggacgccg ccgtggacgg
      841 gcacgtgttc gacgaggacc tggtcatgtt ccgcgacggc atcccggccg actgggcctc
      901 cgacgccgac ctggcctggc gcgtgcggct gatgggctgg cggtgcgtgt acgagccggg
      961 cgccgtggcc caccacatcc gccgctacag cccgtccacg cgcgggcgcg tgccggagtg
     1021 gcagcggatg gtgcagttcc gcaaccgcta cctgatgatg gtcaagaacg acccgctccc
     1081 cgcgctggcg cgcgacctgc cgcggatcct gctctacgag gccttggcgc tgggcttcgt
     1141 cctgctgcgc gagcgcttcc tgctgcgcgg ctacctcgag gccgcgcggg cgttcccgcg
     1201 catgcgcgcc aagcgccgcg tgctccagcg ccggcggcgc gcgcgcgagg ccccggcccc
     1261 tccctacggg ctgctgcccc cggcctgatg tctaccctgc cctccatgga agcggggtcc
     1321 gcgccggcgg acgacaccac ggcgctgttc gacatccgga tccagacggc gccgccggcc
     1381 gagatgctgc gcgcgatcgt gggctacgcg cgcgacggcg agcgccgccg cgtgagctac
     1441 gtgaacgccc acgtgctcaa ccagtccttc gccgaccccg agctgcgccg cgccctgcgc
     1501 gaatcggacc tggtctactg cgacggctac ggggtgcggc tggccgcccg gatgatcggg
     1561 ctgcccgtcc cccaccggat gaccggcgcc gactgggtgt ggaccgtggc cgcgatgtgc
     1621 caggaggagg gcctgtcgct gtacctgctg ggctccgagc cgggggcctc ggccgacgcc
     1681 gccgccgcgc tccggcgctg gtaccccgag ctcaaggtcc acggcaccca ccacggctac
     1741 ttcggcctcg gcacccctca cgcggagcgc gtgatcgagc acctggccga gaccaagccg
     1801 gacatcctgc tggtgggcat gggcaccccg cagcaggaac tgtgggtgga gcagaaccag
     1861 gaccggatcg aggcgtcggt ggtgtggacc gtcggcgcgc tgttcgacta cgtgtccgac
     1921 cgcgtccccc gcgcgccgcg ctggatcgcc gacaacggct tcgagtggat cttccggctg
     1981 gccgtcgagc cgcgccggat gtggcgccgc tacctgctgg gcaaccccgc cttcctgtac
     2041 cgcatcctcg aggagcggcg gcggccggga cgatgagcag cgcggccgtc cgcgctccag
     2101 ggggaggccc ccgccaggac gcgcccgcga tgccggcgcg cgcagcggcg gtgattgccg
     2161 cggtgctggc gctggccgcg ctgtcgatcg cggtgtacgc cggcgtcaag ggcgtggccg
     2221 gggtgcccga cgcggcgcgc ggcgtcctgg ccaccctcct gctgttcggc gtcactggct
     2281 acgcgccggc agtcctgctg ctcccgcgcg cgctgcgccc gcacctgccg ttcttcgccc
     2341 tggggctcgg cgccgcggtc agctgcctgg gcctgtcggt gctcggcttc ttcggcgcgc
     2401 cgctcgtggc gtcgctggtg gtgctcctgg gcggcggcct cgtggcgggc ctgctcctgc
     2461 gcgtccggcg cggccccgct cgcgcggacg acgccgacat ggccgcggcg ggcgggcgca
     2521 cggtcgcctg ggcgtggccg gcgtacatcg cgctgatcct ggtcgcgctg tgcctgatcc
     2581 ccaccttccg cgccggctac ggctcggtcc ccggcaccaa ccccgacggg atgctcagcg
     2641 tgggcacggc cgagttcctc cagcacaacc cgccgcgcgc ggtcgacccc gggctccccg
     2701 tggaccagat gccaccggtc tggcgctcga agtacccgat ctactacggc ctggccggcg
     2761 cggccacgct cagcggcctg gacaccgtgg ccgcctacac cggcttcgcg gcggtggtca
     2821 cggcgctggc ggcgctgggc ttcatgctgc tggcccgcta cgcgctgcgc gctccaccgg
     2881 tggtcgccct ggtggcgctg gcgctggtgg cgttcgcgcg cgtggtcccg tacctggcca
     2941 tccaccccta cttcaaccag ctgtggggga cgctggcgct gggcttcatg ctgctgttcg
     3001 gcctgcggtt cctggacgac cccaaccgcc gtgacgccat cgccgcgctc ctgttcgtcg
     3061 ccctgggcct gtcggcctac ccgctgatgg tggtgttccc ggcgctggcg ttcgtggccg
     3121 ccgccgtgtc gctgcgccgc cgcgggcacg gcgggtggat ccgggggctg ggcctgcccc
     3181 ggggacggcg ctccctgctg ctgtgggtgc cgctggggat cctggtggtg ccggcggccc
     3241 tgatcgtcac gctcggcgtg ctcgagaagt cggccagcgc caccaacctg atgctgggcc
     3301 gcgaggacct caaggcctgg cgcggcgacc tgacctcgtt ccccggcccc gacttctacc
     3361 tcgggccgcc cggcgtcacg ggctacgtgg tggccgcggc ggtgctggtg gtggccttcc
     3421 tgggcctccg gcgcgccccg gccacggcgc gcgcggcgct gggcgcggcc atcgcgggcg
     3481 ggctggtgct ggggctcttc ttccggctgc gcacctacgg cgagtacttc gacctcaagg
     3541 tcctgtcgtt cctgaccccc ctgctcctgg tggccacggt gccgtggctg gccgagcggc
     3601 tgtccggccc gcgcaccgcc gcccgctacg cggtcatggg ggcggtggcc ctggtgctgg
     3661 cgctccaggt cgccgggctg cgtgaggagc tgggacgcac cgcgctccag gtggaccgcg
     3721 agaccttcgt cctgcgggac gtcgcccgcg ggctcccacc gggcagcatc cgcgtggacg
     3781 tcccgccaag cgggcgccac ctgtgggcgt cctacttcct gaacgagcgc cggctgagct
     3841 cctacacccc ggtgctcttc accacctacc cgcacgttcc c
//
LOCUS       DASUPW010000215         4731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_467934, whole genome shotgun sequence.
ACCESSION   DASUPW010000215 DASUPW010000000
VERSION     DASUPW010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467934"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1600
                     /locus_tag="VFY44_04970"
     CDS             <1..1600
                     /locus_tag="VFY44_04970"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5909823.1"
                     /translation="ASLCLDGVTVALAVAALAAALVATPILDASAGDDPLATLTNLAY
                     PVADLMLLAIVIAAFGLTGWRPGRVWLLLGFGLGAMALSDGTYLYQAAKGTYNGGLVD
                     IGWVVAAVLVALAALSPPAPTSPPRLTGARTIVVPTVAALTAVAMETYDHFTRLPTVA
                     ALLSSATLMAVAARMGMAFLENQRMLAASRREASQDALTGLGNRRELNMRLVAATALQ
                     PERGWVRLLILLDLDGFKAYNDSFGHPAGDSLLVRLGARLAQVAAPHGRAFRLGGDEF
                     CVLVDVEPAMCDVVVAGVAEALSERGDGFTIAPSLGTVRLPGEAATPTAALHLADRRM
                     YADKARSRSSATHQTRDVLVSAMREHQPELHEHVTDVAALAARLADELGMPAEERDRI
                     VRAAELHDIGKVAIPDAILNKPGPLNDEEWQFMRRHTLIGERILMAAPALRPVAELVR
                     SSHERWDGQGYPDGLAAEKIPLGARVVTLCDAFEAMVAERPYREAMTEAEALAEIERC
                     SGSQFDPAVVKAFQAVMNRRSAAV"
     gene            complement(1604..2917)
                     /gene="ispG"
                     /locus_tag="VFY44_04975"
                     /gene_synonym="gcpE"
     CDS             complement(1604..2917)
                     /gene="ispG"
                     /locus_tag="VFY44_04975"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX5909824.1"
                     /translation="MASKRQIDVGGVKIGGGAPVAVQSMTKTETADYDATLKQIHAIA
                     DAGADVVRCAVPREKDVEALGRLVKVSPIPIIADIHFNHTLALKAIDAGAHCVRLNPG
                     NIGGPEKVAEVADKAKAAGIPLRIGVNSGSLPKHLHELERENSVEALVQAAVEFVELM
                     ERLEFQDFKVSIKSTSVPNTIASNRLLSQKIDYPLHLGITEAGTKWTGSLKSAVGLGT
                     LLADDIGDTIRISLSTFHAEEEVKVAWEILKALKLREKGPVLIACPTCGRLQFDMDSV
                     VQDIENRLTAYEDAIEVAVLGCAVNGLGEAAHADFGITGAKNEGLIFAHGKPLRKVKQ
                     DDLVDELFAEIDKSIDAGTIVFEEEKAAEGAEWLERIEEENAGELTPERLAALEAKAA
                     TAEAMNTGTELPMAPSNGANRKQVKPKERLDEDVSPTAGRRFTRA"
     gene            3007..4467
                     /locus_tag="VFY44_04980"
     CDS             3007..4467
                     /locus_tag="VFY44_04980"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX5909825.1"
                     /translation="MGAGAALTGASGADAAQAAPETDSPTAAQQRQIRADHLAKAAEP
                     PAPAPPRAPTPAPADPAPAPVKKLTNAPPPPPPPAPEPVAPKPAPPAPTAVREPQIRE
                     RPLPRPPAPVARAAAAPAPAPQPPAPPVTQPTPQRGADGLTPGQRRLIDEDRRAAAAV
                     PPPAPTPPAQVRGDDGLTPGQRRLIDEDARAAQASPPPPPPVDPSTLSPGQRRLIEED
                     RRAAEAQDDPTTPATVMNWNLGRGADYLPASAGTDRDELDDVAATVAEELPDIVTLQE
                     VYTDYTSVSLDDPPDDLEVIVDRIEEETGVRYHVARAPATVKAEDPSGWAPVGGGPFG
                     NAVLSREPFLSEEYEQLPPDGDEGRVTQEVVTTVNGATVTVYNTHLSKDGPPRPAQLE
                     EAFDRAGSVPGPVIFAGDFNVGPDGSDPYARDEGFRRLTEAEGLRCNGTSRAAIDQVL
                     GSNGVRGEQRPGFECGPSDHPYQLVDVEIPETGDPR"
     gene            complement(4464..>4731)
                     /locus_tag="VFY44_04985"
     CDS             complement(4464..>4731)
                     /locus_tag="VFY44_04985"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HEX5909826.1"
                     /translation="GALEGQFAVAAVGVPMGPVTPEAIHEGLKRLAEALAPWGTGLSY
                     LNFTDEPSDASTAFSEADYARLRAVKAELDPERVIRGGQEIEPG"
BASE COUNT          625 a         1773 c         1693 g          640 t
ORIGIN      
        1 cgccagcctc tgcctggacg gcgtgacggt ggccctggcc gtcgccgcgc tcgcggctgc
       61 gctcgtggcc acgccgatcc tggacgcctc ggccggggac gatcccctgg ccacgctcac
      121 gaacctggcc tacccggtgg ccgacctgat gctgcttgcc atcgtgatcg cggccttcgg
      181 gctgaccggc tggcggccgg gccgcgtgtg gctgctgctg ggcttcggcc tgggcgcgat
      241 ggcgctctcc gacggcacgt acctctacca ggcggccaag ggcacgtata acggaggcct
      301 ggtcgacatc ggctgggtcg tggccgccgt cctcgtggcg ctggccgccc tgtcgcctcc
      361 ggcgccgacc tctccgccgc gcctgacggg cgcccgcacg atcgtggtgc ccacggtggc
      421 cgccctgacc gcggtggcca tggagaccta cgaccacttc acgcggctgc cgacggtggc
      481 cgcgctgctg tcctcggcga cgctgatggc ggtggccgcg cgcatgggga tggcctttct
      541 ggagaaccag cggatgctcg ccgccagccg gcgtgaggcg tcgcaggacg ccctcaccgg
      601 cctgggcaac cggcgcgagc tgaacatgag gctggtggcc gccacggccc tgcaacccga
      661 gcggggctgg gtccggctgc tgatcctgct cgacctggac ggcttcaagg cctacaacga
      721 ctcgttcggg catcccgccg gcgactccct cctggtccgg ctcggcgccc gcctggccca
      781 ggtcgccgcg ccccacggcc gcgcgttcag gctcggcggc gacgagttct gtgtcctggt
      841 cgacgtcgag cccgcgatgt gcgacgtcgt cgtggccggg gtcgccgaag cgctgtccga
      901 gcgcggtgac ggcttcacga tcgcgccctc gctcggcacc gtccgcctgc ccggcgaggc
      961 ggcgaccccg acggccgccc tgcacctggc cgaccggcgc atgtacgccg acaaggcccg
     1021 cagccgctcg tccgccacgc accagacccg cgacgtcctg gtctcggcca tgcgcgagca
     1081 ccagcccgag ctgcacgagc acgtgaccga cgtggccgcg ctggcggcgc ggctggcgga
     1141 cgagctgggc atgccggccg aggagcgcga ccggatcgtg cgcgcggccg agctgcacga
     1201 catcggcaag gtcgccatcc ccgacgccat cctgaacaag cccggccctc tgaacgacga
     1261 ggagtggcag ttcatgcgcc gccacacact gatcggcgag cggatcctga tggccgcgcc
     1321 cgccctgcgc ccggtcgccg agctggtccg ctccagccac gagcgctggg acgggcaggg
     1381 ctaccccgac ggcctggccg cggagaagat ccccctgggc gcccgcgtgg tcacgctgtg
     1441 cgacgcgttc gaggccatgg tggccgagcg gccctaccgc gaggcgatga ccgaggcgga
     1501 ggccctggcc gagatcgagc gctgctccgg gagccagttc gaccccgccg tggtgaaggc
     1561 gttccaggcg gtcatgaacc gccgttccgc cgccgtctga ccctcaggcg cgggtgaagc
     1621 ggcggcctgc cgtgggcgag acgtcctcgt ccaggcgctc cttgggcttg acctgcttgc
     1681 ggttggcgcc gttggacggg gccatcggca gctcggtgcc ggtgttcatg gcctcggcgg
     1741 tggcggcctt ggcctccagc gcggccaggc gctccggggt cagctcaccg gcgttctcct
     1801 cctcgatgcg ctcgagccac tcggcgccct cggcggcctt ctcctcctcg aacacgatcg
     1861 tgccggcgtc gatcgacttg tcgatctcgg cgaacagctc gtcgaccagg tcgtcctgct
     1921 tgaccttccg cagcggcttg ccgtgggcga agatcaggcc ctcgttcttg gcgccggtga
     1981 tgccgaagtc ggcgtgcgcg gcctccccca gcccgttgac cgcgcagccc agcacggcca
     2041 cctcgatggc gtcctcgtag gcggtcaggc ggttctcgat gtcctggacc acggagtcca
     2101 tgtcgaactg gagccggccg caggtggggc aggcgatcag gacggggccc ttctcgcgca
     2161 gcttcagcgc cttgaggatc tcccaggcca ccttgacctc ctcctccgcg tggaaggtcg
     2221 agagcgagat ccggatggtg tccccgatgt cgtcggccag cagcgtgccc aggccgaccg
     2281 cggacttcag cgagccggtc cacttcgtgc cggcctcggt gatgcccagg tgcagcgggt
     2341 agtcgatctt ctggctgagc agcctgttcg aggcgatcgt gttgggcacc gaggtggact
     2401 tgatcgacac cttgaagtcc tggaactcca gccgctccat cagctcgacg aactccaccg
     2461 cggcctgcac cagcgcctcg accgagttct cgcgctccag ctcgtgcagg tgcttgggca
     2521 gcgagccgga gttcacgccg atgcgcagcg ggatgccggc ggccttggcc ttgtcggcca
     2581 cctcggccac cttctcgggg ccgccgatgt tgccggggtt caggcggacg cagtgggcgc
     2641 ccgcgtcgat ggccttcagc gccagcgtgt ggttgaagtg gatgtcggcg atgatcggga
     2701 tcgggctgac cttgaccagc cggcccaggg cctccacgtc cttctcacgc ggcaccgcgc
     2761 accggaccac gtccgcgccg gcgtcggcga tcgcgtggat ctgcttcagg gtcgcgtcgt
     2821 agtcggcggt ctcggtcttg gtcatcgact gcaccgcaac cggggcgccg ccgccgatct
     2881 tcacgccgcc gacgtctatc tgccgcttgg aggccatcgc actgaggata ccgaggggcc
     2941 tcgcccccgt ttgggcaggg ctcgtgaagg gaatcgaggg gtatgcgaac gggggccagg
     3001 atcatcgtgg gggcgggagc cgccctgacc ggggcaagcg gcgccgacgc ggcacaggcc
     3061 gcgcccgaga ccgacagccc gaccgcggcg caacagcgcc agatccgcgc ggatcacctg
     3121 gccaaggcgg ccgagccgcc ggcacccgcc ccgccccggg cgcccacccc ggcgccggcg
     3181 gacccggccc cggcgcccgt gaagaagctg accaacgcgc cacccccgcc accgccgccc
     3241 gcgccggagc cggtggcgcc gaagcccgcc ccgccggcgc ccaccgccgt ccgggagccc
     3301 cagatccgcg agcgcccgct tcccaggccc ccggcaccgg tggcccgggc ggccgcggct
     3361 ccggctccgg ccccgcagcc tcccgcaccc ccggtgaccc agccgacccc gcagcggggc
     3421 gccgacggcc tcacccccgg gcagcggcgc ctgatcgacg aggaccgccg cgccgccgca
     3481 gcggtgccgc cgcccgcgcc cacgcccccg gcgcaggtgc ggggcgacga cggcctgacg
     3541 cccggccaga ggcgcctgat cgacgaggac gcgcgcgcgg cgcaggccag cccaccccca
     3601 cccccgccgg tggatccatc gacgctcagc cccggccagc ggaggctgat cgaggaagac
     3661 cggcgcgccg ccgaagccca ggacgatccg acgacgccgg cgacggtgat gaactggaac
     3721 ctcggccggg gggccgacta cctgcccgcg agcgcgggaa ccgaccgcga cgagctcgac
     3781 gacgtggccg cgacggtggc ggaggagctg ccggacatcg tgacgctcca ggaggtgtac
     3841 acggactaca cctcggtctc gctggacgac ccaccggacg atctggaggt catcgtggac
     3901 cggatcgagg aggagaccgg cgtccggtac cacgtggcgc gagccccggc cacggtgaag
     3961 gcggaggacc cgtcgggatg ggccccggtg ggtggcggcc cgttcggcaa cgcggtgctg
     4021 tcccgcgagc ccttcctctc cgaggagtac gagcaactgc cccccgacgg cgacgaggga
     4081 cgggtcacgc aggaggtggt cacgaccgtc aacggagcga cggtgacggt ctacaacacc
     4141 cacctgagca aggacgggcc cccgcgtccc gcccagctgg aggaggcgtt cgaccgagcg
     4201 gggtcggtcc ccggtccagt catcttcgcg ggtgacttca acgtcggacc cgacgggtcg
     4261 gatccgtacg cgcgtgatga ggggttccgc cgcctcaccg aggcggaggg gctgcgctgc
     4321 aacgggacct cacgagcggc gatcgaccag gtgctgggct ccaacggggt ccgaggcgag
     4381 cagcgaccgg gcttcgagtg cgggccctcg gaccatccct accagctggt cgacgtcgag
     4441 atccccgaga cgggcgatcc ccgctagccc ggctcgatct cctggccccc gcggatcacg
     4501 cgctcggggt ccagctcggc cttgaccgcg cgcagccgcg cgtagtcggc ctccgagaag
     4561 gcggtcgagg cgtcgctggg ctcgtcggtg aagttcaggt acgagagccc ggtgccccac
     4621 ggcgcgagcg cctcggccag ccgcttgagg ccctcgtgga tggcctcggg cgtgacgggg
     4681 cccatcggga caccgaccgc ggcgaccgcg aactggccct cgagcgcccc c
//
LOCUS       DASUPW010000216         5854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_743841, whole genome shotgun sequence.
ACCESSION   DASUPW010000216 DASUPW010000000
VERSION     DASUPW010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5854
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743841"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VFY44_04990"
     CDS             complement(<1..614)
                     /locus_tag="VFY44_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909827.1"
                     /translation="MAALLTRLAAVVAPPFCWGCGADTGRGDPLCRACRGALRWLGGD
                     PVRLQGVPLWSAVAYEGPARALVRALKYRGAPGLADPLAAAIAAGAPRDLLEPGSALV
                     PVPMPAARRRRRGYNQAERIATALGRRTGLDVAGCLERGAAARQVGRGRQQRLLEAAS
                     GVRAVASPPRRALLVDDVATTGATLAACATALRRAGTGSVAAVT"
     gene            673..1545
                     /locus_tag="VFY44_04995"
     CDS             673..1545
                     /locus_tag="VFY44_04995"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX5909828.1"
                     /translation="MDGADLVTVLAASPQLKWLPDPLVLAPLAVLVAIYVRRFRRARR
                     EAGGRGAGWPQALAFAAGMLALVAALASPVDALGEEYLFSAHMVQHVLLGDIAPALLL
                     LSLSRVILRPATRRLMWVERALGPFASPWTGIAIWLVIMYAWHIPAAYDAALENPLIH
                     VLEHAAFFTAGLMVWWPLIQPVPMRRRLTGLHTVAYIGTAKFGLAALGLYLTWSGTVL
                     YDYYESVPRVWGLTAIGDQNTGGAIMMVEQSLTFVIALVVLFSRMLIQSEIDQKRRER
                     LEDSARDQAGAAAP"
     gene            complement(1549..1722)
                     /locus_tag="VFY44_05000"
     CDS             complement(1549..1722)
                     /locus_tag="VFY44_05000"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HEX5909829.1"
                     /translation="MKQFSCGAVVPGCTASFSAESEQEILSQVAAHAQRDHGMDDVPP
                     EVVEQVRANITDA"
     gene            1826..2956
                     /locus_tag="VFY44_05005"
     CDS             1826..2956
                     /locus_tag="VFY44_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5909830.1"
                     /translation="MLRTEPDDVTAPDWPSLLDDAISGRLPLDIHFQPIVDLRRGVVC
                     GYEALSRFPAALGLPPDRWFAAADRLGVGTRLEARAVATILSHRAALAPNCFLSLNLS
                     PDAACSPTLQQLLRDEGDLRGVVLEITERSPVEDYDALVEALAPLRALGATLAIDDAG
                     AGYSSMRHIVTLEPQFVKLDRSLVQDLDNDPRKSAAITALGAFAGEVDAWVVAEGVET
                     PGELSALSSLQMPLAQGFLLGRPGPVMAPLDSDLQRVMRERADHGGGVGGLMQMAITV
                     RPGEDERSAFGGFSPAAEVAVRLDEYDRPVALLVRRGGQIDEQPVMCTDAATPPGELA
                     LRAMTRDPALRFTPAACCDARGRLLGLVPIERLVESLARAGG"
     gene            2966..5524
                     /locus_tag="VFY44_05010"
     CDS             2966..5524
                     /locus_tag="VFY44_05010"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5909831.1"
                     /translation="MGDVHIVGERPPDSTPLPIAAALRRLSFPALAAQDVEGVHATLA
                     QELIEVFAADHAHVTQIAPDRATGRGVQYVPAEGTVTVGATYTHSLDGPSGVVKVALS
                     GVPLVIPDAAGSEDVNQVLVERFGVASAVFLPVIFDEGPLAVAVVCWHEPHEVSSREL
                     ALMMLLSDQCAATLAVMDMRASLNAQAEREAALARAASALNARLDLQAVLETLCHEGA
                     VALNGDVTGFYLGDGEKGGVAVAAHGMAAGSDWYGFVMRPGEGVAGQVLQTGRTVVSN
                     AYQAEVDVPASDELRSVTTAVAVPVRWDDRLKGALSVGFRSMRPVTDVDIETLQAMAD
                     LAAVACSNAEAFERAQVAARTDSLTGLLNHGAVHMRLREEIARVSRTGEPLSCLLADL
                     DRFKPVNDAHGHLVGDQILRQVADAIADEFRAYDGLGRFGGDEFVLVLPGVDTEGATE
                     AAHRLRRSIDRALRDEALAEVTASVGVARWREPLTSGELLDRADRALLVAKRKGKDQV
                     AVANQQTEADLVRLDAGGGEPSALLADLWDMVSQCEHPREVLTRLPALLTESLRLADC
                     VLVHPKLLGDPDALLRRVDGAIARTSLPALREALALTAPLPLLEITGAHAAVALQHDG
                     QLHGLLIMRSDESPFPLASLRLAELISGQAVTALIGQVGGASRTAVGALAAAIDARDN
                     YTHVHSEQVVALATGVARRLGLPEREVELIRDAAMLHDVGKVAIPNEILYKPGPLDEA
                     EWAVMREHPVIGERIILRTPELVAIAPIVRHEHERWDGDGYPDGLGTTDIPVGSRVIL
                     ACDAYNAMITARPYRDPMSDADARAELEEGAGSQFDPVVVAALLAVLTEQAAAL"
     gene            5534..>5854
                     /locus_tag="VFY44_05015"
     CDS             5534..>5854
                     /locus_tag="VFY44_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909832.1"
                     /translation="MEISVAGEGDLDELLPLMRAYCDFYDVAPSDEDLLALSRALIAD
                     PGREGVQLIARGDDGAAARRCGEAARSVGGQPQPDGPQRLSFRGHVHRAGREVGGLAA
                     GKEPD"
BASE COUNT          712 a         2181 c         2206 g          755 t
ORIGIN      
        1 taggtgacgg ccgccaccga gccggtgccc gcgcggcgta gcgcggtcgc gcaggcggcc
       61 agcgtcgccc ccgtggtggc cacgtcgtcg accaggagcg cgcgccgcgg gggcgacgcc
      121 accgcccgga cgccggaggc cgcctcgagc agccgctgct ggcgcccgcg cccgacctgg
      181 cgcgcagccg cgccccgctc gaggcacccg gccacgtcga gcccggtgcg ccggccgagc
      241 gccgtcgcga tccgctccgc ctggttgtag ccccggcgcc gccggcgcgc cgcgggcatg
      301 ggcaccggga ccagcgcgga gccaggctcg agcaggtccc gcggcgcacc cgcggcgatg
      361 gccgcggcca gcgggtcggc caggccgggc gccccgcggt acttgagcgc ccgcaccagc
      421 gcccgggccg ggccctcgta ggcgaccgcc gaccacagcg ggacgccctg gagccggacg
      481 gggtcgccgc cgagccagcg cagggcgccg cgacaggccc ggcagagggg atcgccgcgg
      541 cccgtgtcgg cgccgcagcc ccagcagaac ggcggcgcga cgacggcggc gagacgggtg
      601 agcagggcgg ccatggcgcc agcgtcccgc agcgcgtgtt gcgggtggga tgccggtgcc
      661 acaatcgcgt gcgtggacgg tgccgatctc gtgaccgtgc tggcggcgag cccccagctg
      721 aagtggctgc cggaccccct ggtcctggcc ccgctcgcgg tcctggtcgc gatctacgtg
      781 cgccgcttcc gccgggcccg gcgcgaggcg ggcgggcggg gcgcgggctg gccccaggca
      841 ctggccttcg ccgcggggat gctggcgctg gtggccgcgc tggcgtcgcc ggtggacgcg
      901 ctgggcgagg agtacctgtt ctcggcccac atggtccagc acgtgctgct gggcgacatc
      961 gcgccagcgc tgctgctgct ctcgctctcg cgcgtgatcc tgcgcccggc cacccggcgg
     1021 ctgatgtggg tcgagcgggc actcggcccg ttcgcctccc cctggacggg catcgcgatc
     1081 tggctggtga tcatgtacgc ctggcacatc ccggcggcct acgacgcggc gctggagaac
     1141 ccgctgatcc acgtcctcga gcacgcggcc ttcttcaccg cggggctgat ggtgtggtgg
     1201 ccgctgatcc agcccgtccc catgcggcgg cggctgaccg gcctgcacac ggtggcctac
     1261 atcggcacgg ccaagttcgg gctggccgcg ctcggcctgt acctgacctg gtcgggcacg
     1321 gtgctctacg actactacga gagcgtgccg cgcgtctggg gcctgacggc catcggggac
     1381 cagaacaccg gcggcgcgat catgatggtc gagcagtcgc tgaccttcgt gatcgccctg
     1441 gtggtcctgt tctcgcggat gctgatccag tccgagatcg accagaagcg ccgcgagcgg
     1501 ctcgaggact cggcccgcga ccaggccggc gcggccgccc cctgagcgct acgcgtcggt
     1561 gatgttggcg cgcacctgct cgacgacctc gggcgggacg tcgtccatgc cgtggtcgcg
     1621 ctgggcgtgg gcggcgacct ggctgaggat ctcctgctcg gactcggcgc tgaacgacgc
     1681 ggtgcagccg ggaacgacgg cgccgcatga gaactgcttc atcgtgcttc tcccctgacg
     1741 tggttgaccc ctctgtcgtc ggcacgcgcc cacgccggct tgagcgccct aaaggggcgg
     1801 gcgcaacggt cgatgggaac cagggatgct gcggaccgag ccagacgacg tgacggcgcc
     1861 cgactggccg tcgttgctcg acgacgccat cagcggccgc ctgccgctgg acatccactt
     1921 ccagccgatc gtcgacctgc gccgcggggt ggtctgcggc tacgaggcgc tgtcgcgctt
     1981 ccccgcggcg ctgggcctgc cgcccgaccg ctggttcgcc gcggccgacc ggctcggcgt
     2041 gggcacccgg ctggaggcgc gcgcggtggc cacgatcctg tcccaccgcg cggccctggc
     2101 gcccaactgc ttcctgtccc tgaacctgag cccggacgcc gcctgctcgc ccacgctcca
     2161 gcagctcctg cgggacgagg gcgacctgcg cggcgtggtc ctggagatca ccgagcggtc
     2221 gccggtcgag gactacgacg cactggtgga ggccctggcc ccgctgcggg cgctcggggc
     2281 gaccctggcc atcgacgacg ccggcgccgg ctactcgagc atgcgccaca tcgtgaccct
     2341 ggaaccgcag ttcgtgaagc tcgaccggtc gctggtccag gacctggaca acgacccgcg
     2401 caagagcgcc gccatcaccg ccctcggcgc cttcgccggc gaggtggacg cgtgggtcgt
     2461 ggccgagggc gtggagaccc ccggcgagct gtccgcgctc tcgtcgctgc agatgccgct
     2521 ggcgcagggc ttcctgctcg gccgccccgg gcccgtgatg gcgcctctgg actccgacct
     2581 ccagcgtgtg atgcgcgagc gcgccgacca cggcggcggc gtcggcggcc tgatgcagat
     2641 ggcgatcacg gtccgccccg gcgaggacga gcgatcggcc ttcggcggct tctcgccggc
     2701 cgccgaggtg gccgtgcgcc tggacgagta cgaccgcccg gtcgcgctgc tggtgcgccg
     2761 cggcggccag atcgacgagc agccggtgat gtgcaccgac gccgcaacgc ccccgggcga
     2821 gctggcgctg cgggccatga cccgcgaccc cgccctgcgc ttcacccccg ccgcctgctg
     2881 cgacgcccgc gggcggctgc tggggctggt gccgatcgag cgcctggtcg agtcgctggc
     2941 ccgggcgggc gggtagctca cggccatggg tgacgtccac atcgtcggag agcggccgcc
     3001 ggacagcacg cccctgccca tcgccgcggc cctgcgccgc ctgtcgttcc cggccctggc
     3061 cgcccaggac gtcgaggggg tgcacgcgac cctggcccag gagctgatcg aggtgttcgc
     3121 agccgaccac gcccacgtga cgcagatcgc gcccgaccgg gccaccggcc gcggcgtgca
     3181 gtacgtgccg gccgagggca cggtcaccgt gggcgccacg tacacccact cgctggacgg
     3241 cccctcaggc gtggtcaagg tggccctgtc cggcgtgccg ctggtgatcc ccgacgccgc
     3301 cggctcggag gacgtgaacc aggtgctggt ggagcgcttc ggcgtcgcca gcgcggtctt
     3361 cctgccggtg atcttcgacg aggggccgct ggccgtggcc gtggtctgct ggcacgagcc
     3421 gcacgaggtg tcctcgcgcg agctggcgct gatgatgctg ctgagcgacc agtgcgccgc
     3481 caccctggcc gtcatggaca tgcgcgcgtc gctgaacgcg caggccgagc gcgaggccgc
     3541 gctggcgcgc gccgcgtcgg ccctgaacgc gcggctggac ctgcaggcgg tgctggagac
     3601 gctgtgccac gagggggcgg tggcgctgaa cggcgacgtc accggcttct acctgggcga
     3661 cggcgagaag ggcggtgtgg ccgtggccgc ccacgggatg gcggccggct cggactggta
     3721 cggcttcgtc atgcggcccg gcgagggggt ggcgggccag gtgctgcaga ccggccggac
     3781 cgtggtcagc aacgcctacc aggccgaggt cgacgtcccg gccagcgacg agctgcgctc
     3841 ggtgaccacc gcggtcgcgg tcccggtgcg ctgggacgac cggctcaagg gcgcgctctc
     3901 ggtgggcttc cgctccatgc gccccgtcac cgacgtggac atcgagacgc tccaggccat
     3961 ggccgacctg gccgccgtgg cgtgcagcaa cgccgaggcc ttcgagcgcg cccaggtggc
     4021 cgcccggacc gattcgctga ccggcctcct gaaccacggc gccgtgcaca tgcggctgcg
     4081 cgaggagatc gcgcgcgtca gccggaccgg cgagccgctg tcctgcctgc tggccgacct
     4141 cgaccggttc aagcccgtga acgacgcgca cgggcacctc gtgggcgacc agatcctgcg
     4201 ccaggtggcc gacgcgatcg ccgacgagtt ccgcgcctac gacgggctcg ggcgcttcgg
     4261 cggcgacgag ttcgtgctgg tgctccccgg cgtggacacc gagggcgcga ccgaggcggc
     4321 gcaccgcctg cgccgcagca tcgaccgcgc gctgcgcgac gaggccctgg ccgaggtgac
     4381 cgcgtcggtc ggcgtggccc gctggcgcga gccgctgacc agcggcgagc tcctggaccg
     4441 ggccgaccgg gcgcttctgg tggccaagcg caagggcaag gaccaggtcg cggtcgccaa
     4501 ccagcagacc gaggccgacc tggttcgcct ggacgccggc ggcggcgagc cctccgccct
     4561 gctggccgac ctgtgggaca tggtcagcca gtgcgagcac ccgcgcgagg tgctgacgcg
     4621 gctgcccgcc ctgctgaccg agtcgctgag gctggccgac tgcgtcctgg tgcaccccaa
     4681 gctgctcggc gaccccgacg cgctcctgcg ccgggtggac ggcgccatcg cccgcacgtc
     4741 gctgcccgcg ctgcgcgagg ccctggccct gaccgccccg ctgccgcttc tggagatcac
     4801 gggcgcgcac gccgcggtgg cgctccagca cgacggccag ctgcacggcc tgctgatcat
     4861 gcgctccgac gagtcgccgt tcccgctggc ctcgctgcgg ctggccgagc tgatcagcgg
     4921 tcaggcggtg accgcgctga tcgggcaggt cggaggtgcc tcccgcaccg cggtcggcgc
     4981 gctggccgcg gccatcgacg cgcgcgacaa ctacacgcac gtgcactccg agcaggtggt
     5041 ggcgctggcc accggcgtcg cccgccggct ggggctgccc gagcgcgagg tggagctgat
     5101 ccgcgacgcc gcgatgctgc acgacgtggg caaggtcgcg atcccgaacg agatcctcta
     5161 caagcccggg cctctggacg aggccgagtg ggcggtgatg cgcgagcacc cggtcatcgg
     5221 cgagcggatc atcctgcgca cgcccgagct ggtggccatc gcccccatcg tccgccacga
     5281 gcacgagcgc tgggacggcg acgggtaccc ggacggcctg ggcaccaccg acatccccgt
     5341 cggcagccgc gtgatcctgg cctgcgacgc ctacaacgcg atgatcacgg cgcggcccta
     5401 ccgcgacccg atgtccgacg ccgacgcgcg cgccgagctg gaggagggcg ccggcagcca
     5461 gttcgacccg gtcgtggtgg ccgcgctgct ggcggtgctc accgagcagg ccgcggcgct
     5521 ctagagtcgg cccatggaga tatccgtggc cggcgagggc gacctggacg agctgctgcc
     5581 gctgatgcgc gcgtactgcg acttctacga cgtggcgccc tccgacgagg atctgctggc
     5641 gctgtcacgg gcgctgatcg ccgaccccgg ccgcgaaggg gtccagttga tcgcccgcgg
     5701 cgacgacggc gccgccgccc gacggtgcgg ggaggccgcc cggagcgtcg gaggacagcc
     5761 gcagccagac gggccgcaac gactgtcctt ccgtggtcac gtccaccggg ccggccggga
     5821 ggtcggcggg ctcgccgcag ggaaggagcc ggac
//
LOCUS       DASUPW010000217         4796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1991742, whole genome shotgun sequence.
ACCESSION   DASUPW010000217 DASUPW010000000
VERSION     DASUPW010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1991742"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VFY44_05020"
     CDS             complement(<1..755)
                     /locus_tag="VFY44_05020"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5909833.1"
                     /translation="MGVLDGKSAIVTGSARGIGRATAELLAEQGAQVLINDLDGDVAE
                     QAAGEIQGETAVFSGDLTQGDTPDKLVQTAIDAFGKIDIIVNNAGYTIDAPLHKMTDD
                     QFQRMIDIHNVVPFRVLRAAAPHLREPAKKEKEEGTEVFRKIVNVSSISGTFGNAGQA
                     NYSSGKSGVVGLTKTMAKEWGQFKINVNAVAFGFIDTRLTQAKESDNTMEIGGEKVQL
                     GIPEQMRAMASMIIPLGRPGTPEEAAGGIFFLCS"
     gene            822..1523
                     /locus_tag="VFY44_05025"
     CDS             822..1523
                     /locus_tag="VFY44_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909834.1"
                     /translation="MTRRVPCFGVLLIALTFAAPAEASRAPTKAEAKAIKKGFFKGRS
                     AKTTTIRRMRVSTAATGYAAVSYRTDVKTSTVFSPPTPVVLKKGGGKWKPVTAAKTPK
                     KVKKDLKVKAAKSNVKLSGEVSATWTRPARCSSSGVSIYDPGSDLQLSIQQSREEGYG
                     LRDARGVRTVVAVYRNRGTELAYESGAPADATAPSGVFYREPIGGFVNASLAPPPVPE
                     IRPFAVEVKGDWICG"
     gene            1523..2677
                     /locus_tag="VFY44_05030"
     CDS             1523..2677
                     /locus_tag="VFY44_05030"
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5909835.1"
                     /translation="MPSRTGQEVLADVVGRVDPAHVAERMVAVFRAEIAGYRRLPEPV
                     VVNQILAICRDNVELFFGSITDGKAPAEADLARFRESARDRAGEGMPLEDLLHAYRLG
                     GRMGWQSLVDAADEAERPYLLEGAERLMDYIDRVSSVVAQAYLDERQHLVTEEERRLR
                     DLFEALQRGGPLHGGLRDLADRIRFPLAEAYRPFAYALPTGAAYDHAQLAAELRSRGV
                     LALTEGDRVTGLAPPDGDEPVDGRDAILAVGEPTARGHLHDALEELRLLVDLGRVLGA
                     SGRLEPESFVTELLVARSPRLGALVRSRALGPLEEYAEKRGSDLIDTLETFVASGLDR
                     REAAERLHVHPNTLDYRLGRAAELTGLDLGRPDDLVLTVLALKQRALFSG"
     gene            complement(2667..3641)
                     /locus_tag="VFY44_05035"
     CDS             complement(2667..3641)
                     /locus_tag="VFY44_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909836.1"
                     /translation="MRRILLALTVAAVAGCAPAAQAATVTVAGGTLRVAAPPGEVNAV
                     EVSAGALGSLTIRDTGTDPVAAGACTPTLEPNAVTCPAAGLTRVDADLGDQDDSFSLY
                     APLPGRVRGGPGDDQLYGGGSKDTLDGGSGADTADGGRGDDRVVMRDRKTDTVACGEG
                     RDRARAEVLDELDVSCEIVDYAPAGRIGSLRARTGGGRFVAVPGQVDTRVDRRILPAV
                     LYLVRRYHLRLGDGYKVGGNHKEKGEHPLGLGLDIYPGAGGSWRQIDKLAKWAEPRQN
                     RPRPPFRWVGYDGDYNHGRGNHLHLSWMHSAGRPGRPVRSVWTWAVSR"
     gene            complement(3671..>4796)
                     /locus_tag="VFY44_05040"
     CDS             complement(3671..>4796)
                     /locus_tag="VFY44_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5909837.1"
                     /translation="EEHGELRESIRRFVAAELRPHANEWEEAEWFPDEVFAKLAANGF
                     LGLKYPERYGGEGGDHLHDAVFTEELAGCGSGGVAAGIGAHIGIATPPVFKFGTEEQK
                     QRFLVPAIKGERIAALGITEPDAGSDVAGIKTRAEKVDGGYVVNGSKTFITNGVRADY
                     VVTAVKTTADGGHSGLSFLLMESDMEGFSVSKKLDKMGWRASDTAELAFSDVFVPEEN
                     LLGEENKGFYLIMANFQWERLLMALGAVGSMERVIEATVAYALERNAFGRQIGHFQAI
                     RHKVAEMALKLETGRSLTYHALRLFHDGQDAIKEVTMAKLATQRAAFEVADDAIQIHG
                     GAGYMAEYEIERVARDLRLGPIGGGTDEVMKEILGKQLGL"
BASE COUNT          629 a         1710 c         1735 g          722 t
ORIGIN      
        1 gagcacagga agaagatgcc gccggcggcc tcctccgggg tgcccgggcg gcccaggggg
       61 atgatcatcg aggccattgc gcgcatctgc tcggggatgc ccagctgcac cttctcgccc
      121 ccgatctcca tggtgttgtc cgactccttg gcctgggtca ggcgggtgtc gatgaagccg
      181 aaggccacgg cgttcacgtt gatcttgaac tggccccact ccttggccat ggtcttggtc
      241 aggccgacca cgccggactt gcccgacgag tagttggcct ggcccgcgtt cccgaacgtg
      301 cccgagatcg aggagacgtt cacgatcttg cggaagacct cggtgccctc ctccttctcc
      361 ttcttggccg gctcgcgcag gtgcggggcg gcggcgcgca gcacgcggaa gggcacgacg
      421 ttgtggatgt cgatcatccg ctggaactgg tcgtcggtca tcttgtgcag cggggcgtcg
      481 atggtgtagc ccgcgttgtt gacgatgatg tcgatcttgc cgaaggcgtc gatcgcggtc
      541 tggacgagct tgtccggggt gtcgccctgg gtcaggtcgc cgctgaagac ggcggtctcg
      601 ccctggatct ccccggccgc ctgctcggcc acgtcgccgt cgaggtcgtt gatcagcacc
      661 tgcgcgccct gctcggccag cagctcggcg gtggcgcggc cgatgccgcg tgccgatccg
      721 gtgacgatgg cggacttgcc gtccagtact cccacgttga tctctccttg gtcagctcga
      781 agaactcgcg ggagactatt ggcaggtgta taagaccgct catgactcgt cgagtgccgt
      841 gcttcggggt cctgctcatc gcgctgacgt tcgcggcgcc ggccgaggcg tcacgcgcgc
      901 ccaccaaggc cgaggccaag gcgatcaaga aggggttctt caagggccgg tcggcgaaga
      961 cgacgacgat ccggcggatg cgggtgtcca cggccgccac cggctacgcg gccgtgagct
     1021 accggacgga cgtgaagacc agcaccgtgt tctcgccccc caccccggtc gtgctcaaga
     1081 agggcggcgg gaagtggaag ccggtcacgg cggccaagac gccgaagaag gtgaagaagg
     1141 acctgaaggt caaggcggcc aagagcaacg tcaagctctc cggcgaggtc tcggccacgt
     1201 ggacgcgtcc ggcgcggtgc agctcctccg gcgtgtcgat ctacgacccc ggctccgatc
     1261 tccagctgag catccagcag tcgcgcgagg agggctacgg cctgcgcgac gcgcgtggcg
     1321 tgcgcacggt ggtggccgtc taccgcaaca ggggcaccga gctggcctac gagtccggcg
     1381 cgcccgcgga cgccaccgcc ccgtccggcg ttttctaccg cgagcccatc ggagggttcg
     1441 tgaacgccag cctcgcgccg ccgcccgttc ccgagatcag gccgttcgcc gtcgaggtga
     1501 agggcgactg gatctgcggc tagtgcccag ccgcaccggc caggaggtgc tggccgatgt
     1561 agtgggccgg gtcgatcccg cccacgtggc cgagcggatg gtggccgtct tccgggcgga
     1621 gatcgccggc taccggcgcc tgcccgagcc ggtggtggtc aaccagatcc tggccatatg
     1681 ccgcgacaac gtcgagctgt tcttcggctc gatcaccgac ggcaaggcgc cggccgaggc
     1741 cgacctggcc cggttccgcg agtcggcccg cgaccgcgcc ggggagggca tgccgctgga
     1801 ggacctgctg cacgcctacc ggctcggcgg gcggatgggc tggcagtccc tggtggacgc
     1861 cgccgacgag gcggagcggc cgtacctgct ggagggcgcg gagcggctga tggactacat
     1921 cgaccgcgtc tcctcggtgg tggcgcaggc ctatctggac gagcgccagc acctggtcac
     1981 cgaggaggag cggcgcctgc gtgacctgtt cgaggcactc cagcgcggcg gcccgctgca
     2041 cggcggcctg cgcgacctgg ccgaccggat ccggttcccg ctggccgagg cctaccgccc
     2101 gttcgcgtac gcgttgccga ccggcgccgc ctacgaccac gcccagctgg cggccgagct
     2161 gcgcagccgc ggggtgctgg ccctgaccga gggcgaccgc gtgaccgggc tggcgccgcc
     2221 cgacggagac gagccggtgg acggccgcga cgccatcctc gccgtcggcg agcccaccgc
     2281 acgcggccac ctgcacgacg cccttgagga gctgcgcctg ctggtggacc tggggcgggt
     2341 gctcggcgcg agcggccggc tggagcccga gtcgttcgtc accgagctgc tggtggcccg
     2401 ctcgccccgc ctgggcgcgc tggtgcgctc gcgcgcgctg ggcccgctgg aggagtacgc
     2461 cgagaagcgt gggtcggacc tgatcgacac gctggagacg ttcgtggcca gcgggctgga
     2521 ccggcgcgag gcggccgagc gcctgcacgt gcacccgaac acgctggact accggctggg
     2581 acgggccgcc gagctgaccg gcctggacct gggtcgcccc gacgatctgg tgctgacggt
     2641 gctggcgctc aagcagcgcg cgctgttcag cggctgacgg cccacgtcca gaccgaccgg
     2701 accggtcggc cggggcgccc ggcgctgtgc atccaggaca ggtgcaggtg gttgccgcgg
     2761 ccgtggttgt agtcgccgtc gtagccgacc cagcggaacg ggggccgggg ccggttctgg
     2821 cgcggctcgg cccacttcgc cagcttgtcg atctggcgcc agctgccgcc cgcgcccggg
     2881 tagatgtcca gccccaggcc cagcgggtgc tcgcccttct ccttgtggtt gccgcccacc
     2941 ttgtagccgt cgcccaggcg caggtggtag cggcgcacca ggtacaacac ggccggcagg
     3001 atgcgccggt ccacgcgggt gtcgacctgg cccggcacgg ccacgaagcg ccccccgccg
     3061 gtccgcgcgc gcagcgagcc gatgcgcccg gcgggcgcgt agtccacgat ctcgcaggac
     3121 acgtccagct cgtccagcac ctcagcgcgg gcgcggtcgc gtccctcgcc gcaggccacg
     3181 gtgtcggtct tgcggtcgcg catgaccacg cggtcgtctc cgcggccacc gtcggcggtg
     3241 tcggcgccgc tgccgccgtc gagggtgtcc ttcgagccgc cgccgtagag ctggtcgtcg
     3301 cccgggccgc cgcgcacgcg gcccggcagc ggcgcgtaca gcgagaagga gtcgtcctgg
     3361 tcgcccaggt cggcgtccac gcgcgtcagg cccgccgcgg ggcaggtgac cgcgttcggc
     3421 tccagcgtgg gggtgcaggc gccggcggcc acggggtcgg tgcccgtgtc ccggatcgtc
     3481 agcgacccga gggcgccggc cgagacctcg acggcgttga cctcgccggg cggggcggcc
     3541 acgcgcagcg tgccgccggc gacggtcacg gtggccgcct gggcggccgg cgcgcagccc
     3601 gccacggcgg ccacggtcag ggcgagcagg atccggcgca tccgctctgt gtcggcagca
     3661 gagcggcggg ctaaagcccg agctgcttgc ccaggatctc cttcatgacc tcgtcggtgc
     3721 cgccgccgat cggccccagg cgcaggtcgc gggccacgcg ctcgatctcg tactcggcca
     3781 tgtacccggc cccgccgtgg atctggatgg cgtcgtcggc gacctcgaac gcggcgcgct
     3841 gggtggccag cttggccatc gtgacctcct tgatcgcgtc ctggccgtcg tggaacaggc
     3901 gcagcgcgtg gtaggtcagc gagcggccgg tctccagctt cagggccatc tccgcgacct
     3961 tgtggcggat ggcctggaag tggccgatct ggcggccgaa ggcgttgcgc tccagcgcgt
     4021 aggccacggt ggcctcgatc acccgctcca tcgagcccac cgcgccgagc gccatcaaca
     4081 gccgctccca ctggaagttg gccatgatca ggtagaagcc cttgttctcc tcgcccagca
     4141 ggttctcctc gggcacgaac acgtccgaga aggccagctc ggcggtgtcc gaggcgcgcc
     4201 agcccatctt gtccagcttc ttggagacgc tgaagccctc catgtcgctc tccatgagca
     4261 ggaacgacag gccggagtgc ccgccgtccg cggtggtctt gaccgcggtg accacgtagt
     4321 cggcccgcac gccgttggtg atgaaggtct tcgagccgtt gaccacgtag ccgccgtcca
     4381 ccttctcggc gcgcgtcttg atgccggcca cgtccgagcc ggcgtcgggc tcggtgatgc
     4441 ccagggcggc gatccgctca cccttgatcg cgggcaccag gaagcgctgc ttctgctcct
     4501 cggtgccgaa cttgaacacc ggcggcgtcg cgatcccgat gtgcgcgccg atcccggcgg
     4561 ccacgccgcc cgacccgcaa cccgccagct cctcggtgaa cacggcgtcg tgcaggtggt
     4621 cgccgccctc gccgccgtac cgctcggggt acttcagtcc caggaagccg ttggcggcca
     4681 gcttggcgaa cacctcgtcg gggaaccact cggcctcctc ccactcgttc gcgtgggggc
     4741 ggagctccgc ggccacgaag cggcggatcg actcgcgcag ctcgccgtgc tcctcg
//
LOCUS       DASUPW010000218         3362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1714209, whole genome shotgun sequence.
ACCESSION   DASUPW010000218 DASUPW010000000
VERSION     DASUPW010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1714209"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VFY44_05045"
     CDS             <1..196
                     /locus_tag="VFY44_05045"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEX5909838.1"
                     /translation="PKSDTQASTAPKPKPKPATYSVKAGDNLGSIAEKTQVPVETLQE
                     LNPELDPQALVAGQKIKLKE"
     gene            193..1461
                     /locus_tag="VFY44_05050"
     CDS             193..1461
                     /locus_tag="VFY44_05050"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX5909839.1"
                     /translation="MTARLSRAVLAALVALALAPLAPAQAADPKPNLDAAASAILIDA
                     RDGSVLLAKRPEAERSMASTTKLMTALLTLERTEPDQVFTASGYRAAPIESKISLRPG
                     ERMRVSDLFEGLMLESANDAAVTLAEGISGSRPAFVRDMNERAAALGLRHTRYSNPIG
                     LDEGDNHSSARDLAMLARRLMTNPRFAAVVRQPSAVLESGARRRVVNNRNDLVGRYPY
                     VKGVKTGRTREAGYVLVGAASNAAGARVISVVMGTPSESARDATSLALLQYGLKQFQR
                     RKVLDADTPVASAKVEYRDERTELVPRSDLLVLARRGEKVRTRVDAPEELGETPAGRR
                     VGTIAVLREGKVIDRVPLVTAAEVPGTGPLRRLVNALGGVVPLVLILVFISVIVVVAV
                     RQRSRRRERERAERRRARNRARARAEGQIE"
     gene            1458..2435
                     /locus_tag="VFY44_05055"
     CDS             1458..2435
                     /locus_tag="VFY44_05055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HEX5909840.1"
                     /translation="MIITVTLNAAIDKTLAVPNFRLGRRHRAVEQTSMAGGKGVNVAR
                     ALKAIGRPVIATGFAGGPTGTRIIEQLTDEHILNDFVRIREESRMSTAVVDPTSGEQT
                     EINERGPTVSEGELDLFVDKLLYLAQGAAVCVFSGSLPGGVDPGLYARLVGELRKMGV
                     FTVIDAEGDPMRLATRAGPDVVSPNELEAEELVGHEFADAEDRATAVAEIVDMGAREA
                     LMTMSDGALALLGTDTDATRPRELVRATLDPLEPVSSVGSGDAFLAGWVAAHYENRPA
                     HECLRFGVACGAESTQHFGAGVLDPREVGRLLNEVELEVLDRDALLLGR"
     gene            2471..>3362
                     /locus_tag="VFY44_05060"
     CDS             2471..>3362
                     /locus_tag="VFY44_05060"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5909841.1"
                     /translation="MDQRPGGSPIKRRDVYPNAVVDGQELQQNLPGRLRSRWQSLMVS
                     KGERDEAEIEEQIRTVPGVTRANTVAMISPKGGVGKTTSSFVVGNLLSNTLRMRTIVV
                     DANPDFGTLAALAPDQLRPERNLADLINDLEKVHTAAELGPYVSRLPSGLHLLGAPTD
                     AELMAKLGPEAYGHLLAFLSIFYEVILLDLGTGITDPLARFAVERSDQLVLVTTPEFI
                     TSQSVAGALRYLAHERATLVLNQASGGHEADIKQIEAHFREQRLRRSVTIPRDERLRT
                     MLDTGTYSLGALDRDTRRPVK"
BASE COUNT          512 a         1282 c         1154 g          414 t
ORIGIN      
        1 gcccaagtcg gacacccagg ccagcaccgc ccccaagccc aagcccaagc cggccaccta
       61 ctccgtcaag gcgggcgaca acctggggtc gatagccgag aagacccagg tcccggtcga
      121 gaccctgcag gagctcaatc ccgagctcga cccgcaggcc ctcgtggccg ggcagaagat
      181 caagctcaag gaatgaccgc acggctttcc agggccgtgc tggccgccct cgtggcgctg
      241 gcgctggctc cgctcgcccc tgcccaggcg gccgacccca agccgaacct cgacgctgcc
      301 gccagcgcga tcctgatcga cgcccgcgac ggctccgtgc tgctggccaa gcgcccggag
      361 gccgagcggt ccatggccag caccaccaag ctcatgaccg cgctgctcac gctcgagcgc
      421 accgagcccg accaggtgtt caccgcctcc ggctaccggg cggcgccgat cgagtcgaag
      481 atcagcctgc ggccgggcga gcggatgcgc gtctccgacc tcttcgaggg cctgatgctg
      541 gagagcgcca acgacgccgc ggtcacgctg gccgagggca tctcggggtc acggcccgcg
      601 ttcgtgcgcg acatgaacga gcgcgccgcc gcgctcggcc tgcgccacac gcgctactcc
      661 aacccgatcg gcctggacga gggcgacaac cactccagcg cccgcgacct ggcgatgctg
      721 gcccggcggc tgatgaccaa cccgcgcttc gccgcggtcg tgcgccagcc ctcggcggtg
      781 ctggagagcg gcgcgcgccg gcgcgtcgtg aacaaccgca acgacctggt cgggcgctac
      841 ccgtacgtca agggcgtcaa gaccggccgc acgcgcgagg ccggctacgt gctggtgggg
      901 gcggcctcca acgccgcggg cgcgcgcgtc atcagcgtgg tcatgggcac gccgagcgag
      961 tcggcgcgcg acgccacctc gctcgccctc ctgcagtacg gcctgaagca gttccagcgc
     1021 cgcaaggtcc tggacgccga taccccggtg gcctccgcca aggtcgagta ccgcgacgag
     1081 cgcaccgagc tggttccgcg cagcgacctg ctggtgctgg cccgccgcgg cgagaaggtg
     1141 cgcacgcgcg tggacgcgcc cgaggaactg ggggagaccc ccgccggacg ccgcgtgggc
     1201 accatcgccg tgctgcgcga gggcaaggtc atcgaccgcg tgccgctggt caccgcggcc
     1261 gaggtgccgg gcaccgggcc gctgcggcgg ctggtcaacg ccctcggcgg ggtggtccca
     1321 ttggtgctga tcctcgtatt catttccgtc atagtggttg tggcggtgcg ccaacgatca
     1381 cgccgccggg aacgcgagcg agctgagcgc aggagggcac ggaaccgggc cagagcccga
     1441 gcagaggggc agatcgaatg atcatcaccg tcacactcaa cgccgcgatc gacaagacgc
     1501 tggccgtgcc caacttccgc ctgggccgcc gccaccgcgc ggtcgagcag acctccatgg
     1561 ccgggggcaa gggcgtgaac gtggcccggg cgctgaaggc catcggccgc cccgtgatcg
     1621 ccaccggctt cgccggcggg ccgaccggca cgcgcatcat cgagcagctg accgacgagc
     1681 acatcctcaa cgacttcgtg cgcatccgcg aggagtcgcg catgtccacc gcggtcgtgg
     1741 acccgacctc cggcgagcag accgagatca acgagcgcgg cccgaccgtg tccgagggcg
     1801 agctggacct gttcgtggac aagctcctgt acctggccca gggcgcggcc gtgtgcgtgt
     1861 tcagcggcag cctgcccggc ggcgtggacc ccggcctgta cgcccgcctg gtcggcgagc
     1921 tgcgcaagat gggcgtgttc acggtcatcg acgccgaggg cgacccgatg cgcctggcca
     1981 cccgcgccgg ccccgacgtg gtcagcccca acgagctcga ggccgaggag ctggtgggcc
     2041 acgagttcgc cgacgccgag gaccgcgcca ccgccgtggc cgagatcgtg gacatgggcg
     2101 cccgcgaggc gctcatgacc atgtccgacg gcgcgctggc cctgctgggc accgacaccg
     2161 acgccacccg cccgcgcgag ctggtgcggg ccacgctgga cccgctggag ccggtctcgt
     2221 cggtcggctc gggcgatgcc ttcctggccg gctgggtggc cgcccactac gagaaccgcc
     2281 ccgcccacga gtgcctgcgc ttcggcgtcg cctgcggcgc cgaatcgacc cagcacttcg
     2341 gcgccggcgt cctcgacccg cgggaggtcg gccggctgct gaacgaggtc gagctcgagg
     2401 tgctggaccg cgacgccctc ctgctgggcc ggtagagcca cagcgccccc ggcgctaagg
     2461 tgcgcgcccg atggaccagc gaccgggcgg aagccccatc aagcggcgcg acgtctaccc
     2521 caacgcggtg gtggacggcc aggagctcca gcagaacctg cccggccggc tgcgctcgcg
     2581 ctggcagtcg ctcatggtca gcaagggcga gcgcgacgag gcggagatcg aggagcagat
     2641 ccgcaccgtc cccggcgtca cccgcgccaa caccgtcgcc atgatcagcc ccaagggggg
     2701 cgtgggcaag accacctcca gcttcgtggt gggcaacctg ctgtccaaca cgctgcgcat
     2761 gcgcacgatc gtcgtggacg ccaaccccga cttcggcacc ctggccgcgc tggcgcccga
     2821 ccagctgcgc cccgagcgca acctggccga cctgatcaac gacctcgaga aggtgcacac
     2881 cgccgccgag ctgggcccgt acgtgtcgcg cctgcccagc ggcctgcacc tgctgggcgc
     2941 gcccaccgac gccgagctga tggccaagct cggccccgag gcctacggcc acctgctggc
     3001 cttcctgtcg atcttctacg aggtgatcct gctggacctg ggcaccggca tcaccgaccc
     3061 gctggcccgg ttcgcggtcg agcgctcgga ccagctggtc ctggtcacca cccccgagtt
     3121 catcacctcc cagtcggtgg ccggggcgct gcgctacctg gcccacgagc gcgccacgct
     3181 ggtgctcaac caggcttccg gcgggcacga ggccgacatc aagcagatcg aggcccactt
     3241 ccgcgagcag cggctgcggc gctcggtcac gatcccgcgc gacgagcgcc tgcgcaccat
     3301 gctcgacacc ggcacctact cgctcggcgc actggaccgc gacacccgcc gccccgtgaa
     3361 gc
//
LOCUS       DASUPW010000219         4603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1437437, whole genome shotgun sequence.
ACCESSION   DASUPW010000219 DASUPW010000000
VERSION     DASUPW010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437437"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="VFY44_05065"
     CDS             <1..863
                     /locus_tag="VFY44_05065"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX5909842.1"
                     /translation="KSSRGRISRAPDLGDHMLSTDGVLTRTVADSAVLLDLLAGYETG
                     DATWATPPEASFAEQAAREPGALRIALTFDLPVESELDPQYEQAARDTAKLLEELGHH
                     VEEVDAPWKGVDLLPTFSVLWAANVSVSATFSGMLGGREPGPDTMEPLSMWLYETGRD
                     IAAPDYLLAVGQLHAFSRGVVGFMDNWDVLITPSLAKLPVRHGEIDPLGDNPAYEFKK
                     SGEFTPYTAGMNVTGQPAISLPLGQSEEGLPLGTQLIGRPEDEGTLLALSAQLEQARP
                     WADRVAPELS"
     gene            914..3154
                     /locus_tag="VFY44_05070"
     CDS             914..3154
                     /locus_tag="VFY44_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909843.1"
                     /translation="MAVAIAALAVAGSALAQPLRQSTAQVQINPGGAFDPAKPGFFAL
                     GPGAGQKPNLRELPGARAQANRSKRRTSRTYFAAINDFQLADEESPARIDSLAPVQPN
                     TSAWRPQEALMPQTIDATIRRLNQFTAASPNSGLKGRRARMDLALIGGDNSDNQQENE
                     NTWVRQLLEGGQTLDPNSGIADYSSCTPQQRTALLQRPADEALRYTGLQDYDDYNDGA
                     GDGNFYDPTRPAGVYGAWPQYAGLMDVAQRPFVATGLRNGAAPVPTYLTNGNHDGAVQ
                     GYVSATRSADELATGCFKPYINSPTTNGPVSSVFSASTGFPVPPDPRRRFVDRAALKR
                     IYAAGGQRDAHGFGFVDQGQNAASAGAASYYAWTPKKGLRFVSLDTVSEGSGARGGAE
                     GNLDDPQYQWLRGELRRAKAAKQLVVVFGHHPIRRLIAPTPDEAAGPCTGAPGCDSDP
                     RPSSPIHLRADVERVLNANANVVAYLSGHSHVNRIRPCATRCTKKGNWWSIETTAVAD
                     WPQQTRLMEVMDNEDGTLSLLGTPVDHAGPAALPPPSPDPAATGAFSGDQLASMARAF
                     SYNDPRAVRKAAGGKGDRNVELMVRDPFAGKGAGLCAGVTAKTTGGTVNGAVLGRRRA
                     TVRRAVTKYALKAKSGRVDRYCLVGGGFLRVGFVGDRSAIALSSNRSQRLSKVKVGST
                     VKTVRKRLRGERSYRAGSFTWYVATASRARVVVQTRRGKVVQMGLADKKRTSTRKKTA
                     ALLRGF"
     gene            complement(3113..4165)
                     /locus_tag="VFY44_05075"
     CDS             complement(3113..4165)
                     /locus_tag="VFY44_05075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5909844.1"
                     /translation="MADLKLSVLDQSPISEGSTGSRALHQTIELARLADRLGYHRYWV
                     AEHHGGPMLAGPSPEVLIGPIAAATERIRVGSGGVMLPHYSPFKVAESFSLLAGLFPE
                     RIDLALGRASGTDPMTAHALQRDRTNPMPDDFPNQLAELLAYLEDRLPANHPFARLAK
                     ALPGLPEKPEPWLLGSSQQSAVWAGELGLPYAFADFINPNGAPIAELYRDKFVDSERL
                     AAPSLAIGAWALAADTEEEAQELASSSMMAMRELRAGRLIAVPPVEKALRYLERQDEA
                     VPAGGGRRMTLGTGEQVRAGLEELAAQYGADEVIVVTITHDPEARRHSYELIAGAFGL
                     EAGSEPAEQRGGLLPR"
     gene            4239..>4603
                     /locus_tag="VFY44_05080"
     CDS             4239..>4603
                     /locus_tag="VFY44_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909845.1"
                     /translation="MAERAKKTTAVVTATLGSLAAPSAAEAMLPPDMGGPPPTRDDEP
                     RDAPAPKPAAPAKPAQRDPAPAPAPTPVRDPVQRQQPVRRTEPRPQPLPEPERRVARS
                     DAPPAVASPDQIDRRRDAR"
BASE COUNT          645 a         1770 c         1618 g          570 t
ORIGIN      
        1 tcaagtccag cagggggcgg atctcccgcg ctccggacct gggcgaccac atgctgtcca
       61 cggacggcgt cctgacccgc acggtggccg actcggccgt cctgctggac ctgctggccg
      121 gctacgagac cggcgacgcg acgtgggcca cgccgcccga agcctcgttc gccgagcagg
      181 ccgcgcgcga gccgggcgcg ctgcggatcg ccctgacctt cgacctgccg gtggagtccg
      241 agctggaccc ccagtacgag caggcggcgc gcgacacggc gaagctgctg gaggagctcg
      301 gccaccacgt ggaggaggtg gacgcgccgt ggaagggcgt cgacctgctg cccacgttct
      361 cggtgctgtg ggcggccaac gtgtcggtca gcgccacgtt cagcggcatg ctgggcggcc
      421 gcgagcccgg ccccgacacc atggagccgc tgtccatgtg gctgtacgag accgggcgcg
      481 acatcgccgc gccggactac ctgctggccg tcggccagct ccacgccttc tcgcgcggcg
      541 tggtcggctt catggacaac tgggacgtgc tgatcacgcc gtcgctggcc aagctgccgg
      601 tgcgccacgg cgagatcgac cccctgggcg acaaccccgc gtacgagttc aagaagtcgg
      661 gtgagttcac gccctacacc gccggcatga acgtgaccgg ccagcccgcc atctcgctgc
      721 ccctgggcca gagcgaggag gggctgccgc tgggcaccca gctgatcgga cgccccgagg
      781 acgagggcac gctgctggcg ctgtcggccc agctcgagca ggcccggccg tgggcggatc
      841 gtgtcgcgcc ggaactctcg tagccgttct gtgtttcgct gtccggatgc gccgagccct
      901 gaccaggagc cttttggctg tcgccatcgc cgcgctggcg gtggccggta gcgccctggc
      961 ccagcccctg cgccagtcca cggcccaggt ccagatcaac cccggcggcg cgttcgatcc
     1021 cgccaagccc ggcttcttcg cgctgggacc gggcgcgggc cagaagccga acctgcgcga
     1081 gctgcccggc gcccgcgccc aggccaaccg gtccaagcgg cgcacctcgc gcacctactt
     1141 cgccgccatc aacgacttcc agctggccga cgaggagtcg cccgcccgga tcgactcgct
     1201 ggctcccgtc cagcccaaca ccagcgcctg gcgcccgcag gaggcgctga tgccgcagac
     1261 catcgacgcc accatccggc ggctgaacca gttcacggcg gccagcccca acagcggcct
     1321 caaggggcgg cgggcgcgga tggacctggc gctgatcggc ggcgacaact ccgacaacca
     1381 gcaggagaac gagaacacct gggtgcgcca gctgctcgag gggggccaga cgctggaccc
     1441 caacagcggc atcgccgact actcgtcctg caccccccag cagcgcaccg ccctgctcca
     1501 gcggccggcc gacgaggccc tgcgctacac cggcctccag gactacgacg actacaacga
     1561 cggcgcgggc gacggcaact tctacgaccc gacccgcccc gcgggcgtct acggcgcgtg
     1621 gccccagtac gcgggcctga tggacgtggc ccagcgcccg ttcgtcgcca ccggcctgcg
     1681 caacggcgcc gccccggtgc ccacctacct gaccaacggc aaccacgacg gtgccgtgca
     1741 gggctacgtg tcggccaccc gcagcgcgga cgagctggcc acgggctgct tcaagccgta
     1801 catcaacagc cccaccacca acgggccggt gagctcggtg ttctcggcct ccaccggctt
     1861 cccggtgccg cccgacccgc gccggcggtt cgtggaccgc gccgccctca agcgcatcta
     1921 cgcggccggc ggccagcgcg acgcgcacgg gttcgggttc gtggaccagg gccagaacgc
     1981 cgcctccgcc ggagcggcct cctactacgc gtggacgccg aagaagggcc tgcgcttcgt
     2041 ctcgctggac accgtctccg agggctccgg cgcccgcggc ggcgccgagg gcaacctgga
     2101 cgacccccag taccagtggc tgcgcggcga gctgcgcagg gccaaggccg ccaagcagct
     2161 ggtggtggtc ttcggccacc acccgatccg gcggctgatc gcccccaccc ccgacgaggc
     2221 cgcgggcccg tgcaccgggg cgcccgggtg cgactccgat ccgcgcccgt cgtcccccat
     2281 ccacctccgc gcggacgtgg agcgggtgct gaacgccaac gccaacgtgg tggcctacct
     2341 ctcagggcac tcgcacgtga accgcatccg cccgtgcgcc acccgctgca ccaagaaggg
     2401 caactggtgg tcgatcgaga ccacggcggt ggccgactgg ccgcagcaga cgcggctgat
     2461 ggaggtcatg gacaacgagg acggcacgct gtccctgctg ggcacgccgg tcgaccacgc
     2521 cgggccggcc gcgctgccgc ccccctcccc cgaccctgcc gccaccggcg cgttctcggg
     2581 cgaccagctg gcctcgatgg cccgggcctt ctcctacaac gacccacgcg ccgtgcgcaa
     2641 ggccgcgggc ggcaagggcg accggaacgt ggagctgatg gtgcgcgacc cgttcgcggg
     2701 caagggtgcc ggcctgtgcg ccggcgtgac cgccaagacc accggcggca ccgtgaacgg
     2761 ggcggtgctg ggccgcaggc gggccaccgt gcgccgcgcc gtgaccaagt acgccctcaa
     2821 ggccaagagc ggccgcgtgg accgctactg cctggtgggc gggggcttcc tgcgggtggg
     2881 cttcgtgggc gaccgctcgg ccatcgcgct cagctccaac cggtcacagc gcctgagcaa
     2941 ggtcaaggtg ggctcgaccg tgaagaccgt gcgcaagcgc ctgcgcggcg agcgctccta
     3001 ccgcgccggc tcgttcacct ggtacgtggc caccgcgtcc agggcccggg tcgtggtcca
     3061 gacccggcgc ggcaaggtcg tgcagatggg gctggccgac aagaagcgga cgtcaacgcg
     3121 gaagaagacc gccgcgctgc tccgcgggtt ctgagccggc ctccaggccg aaggcgccgg
     3181 cgatcagctc gtaggagtgc cggcgtgcct cgggatcgtg ggtgatggtc accacgatca
     3241 cctcgtcggc gccgtactgc gcggccagct cctccagccc cgcccgtacc tgctcgccgg
     3301 ttcccagggt catgcgccgg ccaccgcctg cggggaccgc ctcgtcctgg cgctcgaggt
     3361 agcgcagcgc cttctccacc ggcggcacgg cgatcaggcg cccggcgcgc agctcgcgca
     3421 tggccatcat cgacgacgac gccagctcct gcgcctcctc ctcggtgtcg gccgccagcg
     3481 cccacgcgcc gatggccagg ctcggcgcgg ccagccgctc ggagtccacg aacttgtcgc
     3541 ggtagagctc ggcgatgggc gcgccgttgg ggttgatgaa gtcggcgaag gcgtagggca
     3601 ggcccagctc gccggcccag acggcgctct gctgcgacga gcccagcagc cacggctcgg
     3661 gcttctcggg caggcccggc agcgccttgg ccagccgtgc gaaggggtgg ttggccggca
     3721 gccggtcctc caggtaggcc aggagctcgg ccagctggtt ggggaagtcg tccggcatcg
     3781 ggttcgtgcg gtcgcgctgc agagcgtgcg cggtcatcgg atcggtgccc gaggcgcggc
     3841 ccagcgccag gtcgatgcgc tccgggaaca ggccggccag caggctgaag ctctcggcca
     3901 ccttgaacgg gctgtagtgg ggcagcatca cgccgccgct gcccaccctg atgcgctcgg
     3961 tggccgccgc gatcgggccg atcagcacct cggggctcgg ccccgccagc atcggcccgc
     4021 catggtgctc ggccacccag tagcggtggt agccgaggcg gtcggccagg cgcgcgagct
     4081 cgatggtctg gtgcagcgcg cgcgacccgg tcgagccttc cgagatcggt gactggtcca
     4141 ggacgctgag cttcaggtcg gccatgctca cgacgttaga ggtgttgatt cgtccacccc
     4201 gcgtttcacc cgccccctgt cctggggacc ctctccacgt ggcggaacgc gcgaagaaga
     4261 cgacggccgt ggtcacggcg accctgggca gcctggcggc gccgtcggcg gccgaggcga
     4321 tgctgccgcc cgacatgggc gggcccccgc cgaccaggga cgacgagccg cgcgacgccc
     4381 ccgcgcccaa accggccgcc cccgccaagc cggcccagcg cgaccccgcg ccggcgcccg
     4441 ccccgacccc cgtccgcgac ccggtccagc gccagcagcc ggtccgccgg accgagccgc
     4501 ggccgcagcc ccttcccgag cccgagcggc gcgtggcccg ctcggacgct ccgccggcgg
     4561 tggcctcccc ggaccagatc gaccgccggc gcgatgcccg gaa
//
LOCUS       DASUPW010000220         3541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_884008, whole genome shotgun sequence.
ACCESSION   DASUPW010000220 DASUPW010000000
VERSION     DASUPW010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884008"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2040
                     /locus_tag="VFY44_05085"
     CDS             <1..2040
                     /locus_tag="VFY44_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909846.1"
                     /translation="DDGLTQLERTSPVRAFTFVLPKSWTESNLKLVATAIGAKGPANQ
                     NVVLCLSTSCGNPSETLFSVRFAEPPKASEMFLNALQQPVYHPKDRSTTYVGPSAPRI
                     FEKLVAAYPGTIWWLDPTNTGWRTPRFRAFDVATDTAILEPAIAWDKRIGQPGNGTIG
                     TFVQGENPGIRRGRVAVASARTIGGDTLWRPMTSVAHEVFHVFGFPHAAPCKDGNGKG
                     SGGEAWPLADGRLGGVGLDTTPGSGGKSGPFRVIPDRGPTAPAHDLMSYCAKFGNNDP
                     TTWISVYNWSRAMRRTSGALPPISRISRSGGRASASRAGRTAAVDELAVMARGDAAGV
                     RVTSVDRATRPSQGGTPSTFRLVGRNAAGQVQGVAALTQDDLDGPAGSPPSALLSGQL
                     PRAGVTRVDIVDQTGRTVATQATAATKPTARLVSPRRGSRTGRGANLSVRWRTGPGAQ
                     AFRAVIETSANGGRTFQRVWGGPNTGRARIPIGLLRASRAARIRLRVGDGFQEAQATS
                     GRFTLVPRRPSVRIAEPVRGQVVDAGGAVRLFGSATDDRGREIGGRRLRWTAGRTVLG
                     RGADISAALPAGTRRVRLTATSRGRRGSASVAVRVRASTPYFLRLRAKRRGTKTTLTV
                     AASQPARLSVRGKRYGVTTRTKRIRVRVGRKKAVRLRLILSAGGKRSTNTITVRR"
     gene            complement(2041..2880)
                     /gene="mutM"
                     /locus_tag="VFY44_05090"
     CDS             complement(2041..2880)
                     /gene="mutM"
                     /locus_tag="VFY44_05090"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX5909847.1"
                     /translation="MPELPEVETVRRQLAPHLTGRRIEAASVLDPRWCEPAPPEQVQD
                     AVSGRVIESVDRRGKYLTFALEDEVHLVMHLRMTGNLLFVPGGHEDEPRFLRVRFQLD
                     DGNCVLFTDQRRFGTGVVLLGDDSLAEYYDARLGVEPLGDDFTPEALHALARGRRAPV
                     KAFLLNQERIAGVGNIYADEALYRARIHPLKPVGTLRRPKLAALRDAVVESLQLGIDS
                     KGATIDDFRNADGAYGSFQDRFLVHLREGEPCGRCGTTIRKIRAAGRGTYFCPKCQPR
                     PRV"
     gene            complement(2880..3461)
                     /locus_tag="VFY44_05095"
     CDS             complement(2880..3461)
                     /locus_tag="VFY44_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909848.1"
                     /translation="MRPRPILLAALAAGVLPAAGCGEQRPPVATGPVAPSAKTVEYEF
                     PRAGMTLRLPANLQVDRQARLPAVFRAGLVEPFVSAFAYRRAEQVPRNARELAAARRR
                     LVRVTQGRDKGYRLIRSRPTEVGGARALELLGEQTLGRTRLRIRSLHVYRGRAEYVIE
                     IGAPRGQFARFDRGVTPVIERTLDVSGRVARRN"
BASE COUNT          475 a         1345 c         1289 g          432 t
ORIGIN      
        1 gacgacgggc tcacccagct cgaacgcacg tcgccggtca gggcgttcac cttcgtgctg
       61 cccaagtcgt ggacggagag caacctgaag ctcgtggcca cggcgatagg agcgaagggc
      121 ccggccaacc agaacgtcgt cctgtgcctg tccacgtcct gcggcaaccc ctccgagacg
      181 ctcttctccg tccgcttcgc ggagcccccg aaggcgagcg agatgttcct gaacgcgctg
      241 cagcaaccgg tctaccaccc caaggaccgc tcgaccacgt acgtcggccc gtcggcgccg
      301 cgcatcttcg agaagctcgt ggccgcctat cccggcacga tctggtggct ggacccgacc
      361 aacacgggct ggcgtacgcc gcgtttccgg gcgttcgacg tcgccaccga cacggcgatc
      421 ctcgagcctg ccatcgcctg ggacaagcgc atcggccagc cgggcaacgg cacgatcggc
      481 acgttcgtgc agggcgagaa ccccgggatc cggcgcggac gcgtcgccgt ggccagcgcc
      541 cggaccatcg gcggcgacac gctgtggcgc ccgatgacct ccgtggccca cgaggtcttc
      601 cacgtgttcg gcttcccgca cgcggcaccg tgcaaggacg ggaacggcaa gggcagcggt
      661 ggcgaggcct ggccgttggc cgacggccgc ctgggtggcg tcgggctgga caccacaccg
      721 ggctccgggg gcaagagcgg tcccttccgg gtgatcccgg atcggggccc cacggccccg
      781 gcccacgacc tgatgagcta ctgcgccaag ttcggcaaca acgaccccac gacctggatc
      841 tcggtctaca actggagccg ggcgatgcgc cgcacctccg gcgccctgcc gccgatctcc
      901 cgcatcagcc ggagcggtgg ccgcgcgagc gccagccggg ccggccggac cgccgccgtc
      961 gacgagctgg ccgtgatggc ccgcggggac gccgccggcg tgcgcgtgac ctcggtggat
     1021 cgcgcgaccc gtccctccca gggcgggacg ccctccacct tccggctggt gggacggaac
     1081 gcggccggcc aggtccaggg cgtggcggcc ctgacccagg acgacctgga cggcccggcc
     1141 gggtcgcccc cttcggccct gctcagcggc cagcttcccc gcgccggcgt cacccgggtg
     1201 gacatcgtcg accagacggg ccggacggtg gccacccagg ccacggcggc caccaagccc
     1261 acggcccggc tggtctcgcc ccggcgcggc tcgcgcacgg gacggggggc gaacctcagc
     1321 gtgcgctggc gcacgggccc gggcgcccag gccttccggg cggtgatcga gacgtcggcc
     1381 aacgggggac gcaccttcca gcgcgtctgg ggcggcccga acacgggccg cgcccggatc
     1441 ccgatcggcc tgctccgcgc gagccgcgcg gcccggatcc ggctacgagt cggcgacggc
     1501 ttccaggagg cccaggccac ctccggccgc ttcaccctgg tcccccgtcg cccgagcgtg
     1561 cgcatcgccg agcccgtgcg cggccaggtg gtcgacgccg gcggagccgt ccggctgttc
     1621 gggtccgcca ccgacgaccg cggccgtgag atcggcggcc ggaggctgag atggaccgcg
     1681 ggccgcacgg tcctgggccg cggcgccgac atctccgcgg cgcttccggc gggtactcgg
     1741 cgcgtccgtc tgaccgcgac gtcgcgcggg cggcgcggat ccgcctcggt ggccgtccgc
     1801 gtgcgggcca gcaccccgta cttcctgcgc ctgcgggcca agcgccgggg aaccaagacc
     1861 acgctgaccg tggccgccag ccagccggcc cggctgtcgg tgcgcgggaa gcgctacggc
     1921 gtgaccaccc gcaccaagcg gatccgcgtc cgggtgggtc gcaagaaggc ggtcaggctg
     1981 cgcctgatcc tgtcggccgg cggcaagcgc agcaccaaca ccatcaccgt ccggcgctga
     2041 tcagacccgg ggccggggct ggcacttcgg gcagaagtac gtgccacggc cggccgcgcg
     2101 gatcttgcgg atcgtcgtgc cgcaccgccc gcagggctcg ccctcgcgca ggtgcaccag
     2161 gaaacggtcc tggaagcttc cgtacgcgcc gtcggcgttg cggaagtcgt cgatggtggc
     2221 gcccttggag tcgatcccca gctgcagcga ctcgacgacc gcgtcgcgca gcgcggccag
     2281 cttggggcgg cgcagggtcc ccaccggctt cagcggatgg atgcgggccc ggtagagcgc
     2341 ctcgtcggcg tagatgttgc ccacccccgc gatccgctcc tggttgagga ggaaggcctt
     2401 gaccggcgcc cgccgtcctc gcgcaagggc gtgcagcgcc tcgggcgtga agtcgtcgcc
     2461 cagcggctcc acgcccagcc gggcgtcgta gtactcggcc agcgagtcgt cgcccaggag
     2521 cacgacgccg gtgccgaacc ggcgctgatc ggtgaacagc acgcagttgc cgtcgtccag
     2581 ctggaagcgc acgcgcagga agcgcggctc gtcctcgtgg ccgccgggga cgaacagcag
     2641 gttcccggtc atgcgcaggt gcatgaccag gtggacctcg tcctccaacg cgaaggtcag
     2701 gtacttgccg cgccggtcca cggactcgat cacccgtccc gacacggcgt cctggacctg
     2761 ctccggcggt gccggctcgc accagcgcgg gtccagtacg gacgcggcct cgatccggcg
     2821 cccggtcagg tggggcgcca gctggcggcg cacggtctcg acctcgggca gctcgggcat
     2881 cagttgcggc gggccacgcg gcccgagacg tccagcgtcc gctcgatcac cggcgtgacc
     2941 ccgcggtcga agcgggcgaa ctggccccgc ggcgccccga tctcgatcac gtactcggcc
     3001 cggccccggt acacgtgcag gctgcggatg cgcaggcggg tccgccccag cgtctgctcg
     3061 cccagcagct ccagcgcgcg ggcgccgccc acctcggtgg gccgcgagcg gatcagccgg
     3121 taacccttgt cgcggccctg ggtcacgcgc accagccggc ggcgggcggc ggccagctcg
     3181 cgggcgttgc gcggcacctg ctcggcacgc cggtaggcga acgccgacac gaacggctcg
     3241 accaggccgg cccggaacac cgccggaagg cgggcctggc gatccacctg caggttggcc
     3301 ggcagccgca gggtcatgcc cgcgcgcggg aactcgtact cgacggtctt ggcgctcggc
     3361 gccaccgggc cggtggccac gggcgggcgc tgttcgccgc agccggccgc cgggagcacc
     3421 ccggccgcca gcgccgcgag aaggatcggg cggggacgca cgcccgcggt tgtatcagcg
     3481 agcggccgcg cgcgggtgcg cgcggaagta cacgtccttg agcctctcgc tcgagaggtg
     3541 c
//
LOCUS       DASUPW010000221         2131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1714623, whole genome shotgun sequence.
ACCESSION   DASUPW010000221 DASUPW010000000
VERSION     DASUPW010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1714623"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="VFY44_05100"
     CDS             <1..968
                     /locus_tag="VFY44_05100"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX5909849.1"
                     /translation="IVTDTSHTKARILDVRADRIAAALDEGKVVLVAGFQGVSTDSDV
                     TTLGRGGSDTTAVALAAALEADVCEIYTDVAGVFSADPRIVPDARKLSVVSFEEMLEM
                     SASGAGVLQLRSVEYARNHNLPIHCRSSFDDAPGTFVQSEEVTMERPLVTAITHSTDE
                     ARITLTKLPDKPGIAGRVLTVLADSNMNVDMIIQNEPESDQRADMSFTVPRDDLPTAI
                     DALEAVREELGIEAVTSDPDMAKVSVVGAGMKSHPGVAAKTFSVLGQAGVNIEMISTS
                     PIKISCVIREEHVKTAVLELHAAFELGDGTIHREDVRGDHRPVVS"
     gene            1003..>2131
                     /locus_tag="VFY44_05105"
     CDS             1003..>2131
                     /locus_tag="VFY44_05105"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX5909850.1"
                     /translation="MGSTILGVLRERGFPADEVVPLASARSAGRNIDWGDRTLTVQEL
                     TDESLQGFDLALFSAGGSISAEWAPKFADAGAVVVDNSSYWRMHDDVPLVVAGVNPDA
                     IQDHQGIVANPNCTTMQAMMPLKPILDTVGIERIILSSYQAVSGTGQKAVEELHDQAR
                     AVIEAQELPAPSVYPHQIAFNVVPQVESFKDGDDYTTEERKVMAETRKILGAPGIGIT
                     ATCVRVPVYTGHSESINLQTRDPFSPEECRELLAATPGVQVIDDPANGLYPMAIDAAG
                     QDDVFVGRIRRDDSHERCLNLWVVGDNLRKGAATNAVQVAELLVERGLLRGAAAGAAG
                     TTALNAVTYLDMVVRGRPASDAPERVVGKLAGRAGVEMPGGR"
BASE COUNT          346 a          758 c          744 g          283 t
ORIGIN      
        1 ggatcgtgac cgacacctcg cacaccaagg cccggatcct ggacgtgcgc gcggaccgga
       61 tcgcggccgc cctggacgag ggcaaggtcg tgctggtggc cggcttccag ggcgtgtcca
      121 ccgactccga cgtgaccacc ctgggccggg gcgggtcgga caccaccgcc gtcgccctcg
      181 cggccgcgct ggaggccgac gtgtgcgaga tctacaccga cgtggccggc gtgttcagcg
      241 ccgacccgcg gatcgtgccc gacgcgcgca agctcagcgt ggtctcgttc gaggagatgc
      301 tggagatgtc ggcctcgggc gccggggtgc tgcagctgcg gtcggtcgag tacgcccgca
      361 accacaacct gcccatccac tgccggtcca gcttcgacga cgcacccggt accttcgtac
      421 agtccgagga agtcaccatg gaacgaccac tagtcaccgc catcacccac tccaccgacg
      481 aggcgcggat cacgctgacg aagctgcccg acaagcccgg catcgccggc cgggtgctca
      541 cggtgctggc cgacagcaac atgaacgtgg acatgatcat ccagaacgag ccggagtccg
      601 accagcgcgc ggacatgtcg ttcaccgttc cccgtgacga cctgcccacg gccatcgacg
      661 cgctggaggc cgtgcgcgag gagctgggca tcgaggccgt gaccagcgac ccggacatgg
      721 ccaaggtgtc ggtggtcggc gccggcatga agagccaccc gggcgtggcg gccaagacct
      781 tcagcgtgct gggccaggcg ggcgtgaaca tcgagatgat ctcgacctcg ccgatcaaga
      841 tctcgtgcgt gatccgcgag gagcacgtca agaccgccgt gctggagctg cacgcggcgt
      901 tcgagctggg cgacggcacg atccaccgcg aggacgtccg cggcgatcac cggccggtgg
      961 tgagctgacg tgcgcctggc ggtcgtaggc gcgaccggag cggtggggtc cacgatcctc
     1021 ggcgtcctgc gcgagcgcgg gttccccgcc gacgaagtcg tgccgctggc gtccgcgcgc
     1081 tcggccggcc gcaacatcga ctggggcgac cggaccctga cggtccagga gctcaccgac
     1141 gaatcgctcc agggcttcga cctggcgctg ttctcggccg gcggctcgat cagcgccgag
     1201 tgggcgccca agttcgccga cgccggagcc gtggtggtgg acaactcctc ctactggcgc
     1261 atgcacgatg acgtgccgct ggtggtggcc ggggtcaacc ccgacgcgat ccaggaccac
     1321 cagggcatcg tggccaaccc caactgcacg accatgcagg cgatgatgcc gctgaagccg
     1381 atcctggaca cggtgggcat cgagcggatc atcctgtcca gctaccaggc cgtctccggg
     1441 accgggcaga aggccgtcga ggagctgcac gaccaggcac gcgcggtgat cgaggcccag
     1501 gagctgccgg cgccttccgt ctacccgcac cagatcgcct tcaacgtggt gccccaggtc
     1561 gagtcgttca aggacggcga cgactacacc accgaggagc gcaaggtcat ggccgagacg
     1621 cgcaagatcc tgggcgcgcc cggcatcggc atcaccgcca cctgcgtccg cgtgcccgtc
     1681 tacacgggcc actccgagtc gatcaacctc cagacccgcg accccttctc gcccgaggag
     1741 tgccgcgagc tgctggccgc caccccgggc gtccaggtca tcgacgaccc ggccaacggc
     1801 ctctacccga tggccatcga cgcggccggc caggacgacg tgttcgtggg ccgcatccgc
     1861 cgcgacgact cacacgagcg ctgcctgaac ctgtgggtgg tgggcgacaa cctgcgcaag
     1921 ggcgccgcca ccaacgcggt ccaggtggcc gagctgctgg tcgagcgcgg gctgctgcgc
     1981 ggcgccgcgg cgggcgccgc cgggaccacg gcgctcaacg ccgtgaccta cctcgacatg
     2041 gtcgtacggg gtcgtccggc tagcgacgcg ccggagcggg tcgtcgggaa actggccggc
     2101 cgggcgggcg tggagatgcc cggtggacgc a
//
LOCUS       DASUPW010000222         3023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_469260, whole genome shotgun sequence.
ACCESSION   DASUPW010000222 DASUPW010000000
VERSION     DASUPW010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469260"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..1625
                     /locus_tag="VFY44_05110"
     CDS             123..1625
                     /locus_tag="VFY44_05110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5909851.1"
                     /translation="MADTETHTDPHYDRRWLILGVIGIAQLMVVLDMTIVNIALPSIQ
                     NDLGFSDDQRQWLITAYALAFGSLLLLGGRISDLFGRKRAFVTGLLGFAAASAIGGAA
                     QSFGVLVGARALQGVFGALLAPAALSLLATIFTTPSERGKAFGVFGAIAGAGGAVGLL
                     LGGALTELDWRWCLFVNLVFAVPAAIAASGLLHKIPSPNRPKLDLPGTAIATTGLLAL
                     VYGFAHAENSGWTDPITLGLLAGAAVLLAGFVALERRVANPLLPLRIVLDRNRGGGYL
                     AMGMAAVGVFGVFIFLTYYMQQILGFSPIQTGLAFMPMNLGIVTTAMVVNTRLLARTG
                     PRPLIPTGMVLASVAMLLLTRTGVDSAYASHVLPSLILLGVGFGLTFAPVFSGATEGV
                     EPRDSGIASAMVNTSQQVGGAVGTALLSTLVVSATTSFAEGKAPSPALAAEAAVHGYH
                     VAFWVSAGIFALAAVVTGSLLRSGPPLSTLHAREIAAAEAEDRDAVLVAG"
     gene            1668..2645
                     /locus_tag="VFY44_05115"
     CDS             1668..2645
                     /locus_tag="VFY44_05115"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5909852.1"
                     /translation="MSARAWTSFAAVSVLWGIPYLFIKVAVEDGVPPAFLAWARVLLA
                     ALVLCALAWHRGLFASLRGRWRWVVFYAVVEISIPFPLIAAGEQHVSSSLAAILIAAV
                     PLIIAVLAIRFDADERVRGARLIGLLMGFTGVVALVGIDVAGRSDELLGAVFILIAAV
                     GYACGPMTLNRKMSDIEPVAMMGASLAVASVVLTPFAFADVPDATPSAEALASVVVLG
                     LLCTAAAFVLFGGLLLEVGPGRAAVITYVAPVVAVGLGVAVLGERPGVGAAAGLLLIL
                     AGSWLSTDGRLPPGMGAWVARVRARRGGRSPERTPAPAAPQAPERIVGA"
     gene            complement(2716..>3023)
                     /locus_tag="VFY44_05120"
     CDS             complement(2716..>3023)
                     /locus_tag="VFY44_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909853.1"
                     /translation="LALIRRAELPQPQTNVVVAGKEVDLYWPEARLGVEVDAFSTHGS
                     AAAFEDDHKLDADLEAANLRIVRFTGRRIRAHPEAVTAQLAAILALRLGGLPPPRRR"
BASE COUNT          321 a         1094 c         1099 g          509 t
ORIGIN      
        1 gtcacgtctg agcgtcttgt tcgtcatctg tatcgaaggg tagtgcagat gcggaggaca
       61 tacctccact tctatgctac ggtaaccgga gcttctgtct ccgcttgatg taaaggatcc
      121 caatggccga caccgagacg cacaccgatc cccactacga ccgccgttgg ctcatcctcg
      181 gcgtcatcgg catcgcccag ctgatggtcg ttctggacat gacgatcgtg aacatcgcgc
      241 tgccgtcgat ccagaacgac ctcggcttct ccgacgacca gcgccagtgg ctgatcaccg
      301 cctacgcgct cgccttcggc tcgctactgc tcctgggcgg gcggatcagc gacctgttcg
      361 ggcgcaagcg cgcgttcgtg accggcctgc tgggcttcgc cgccgcgtcc gccatcggcg
      421 gcgccgcgca gagcttcggc gtgctcgtgg gcgcgcgggc actgcagggc gtcttcggcg
      481 cgctgctcgc gccggccgcg ctgtcgctgc tggccacgat cttcaccacc ccgtccgagc
      541 gcggcaaggc gttcggcgtc ttcggcgcca tcgcgggcgc cggcggcgcg gtcggcctgc
      601 tgctgggcgg cgcgctgacc gagctcgact ggcgctggtg cctgttcgtg aacctggtgt
      661 tcgccgtgcc cgccgcgatc gccgcctcgg ggctgctgca caagatcccc tcgcccaacc
      721 ggcccaagct ggacctgccg ggcacggcca tcgccaccac cgggctgctg gccctggtct
      781 acgggttcgc ccacgccgag aacagcggct ggaccgatcc gatcacgttg ggcctgctgg
      841 ccggcgccgc cgtgctgctc gccgggttcg tggcgctcga gcgccgggtg gccaacccgc
      901 tgctgccgct gcggatcgtc ctggaccgca accggggcgg tggctacctg gcgatgggca
      961 tggccgccgt cggcgtgttc ggggtgttca tcttcctgac ctattacatg cagcagatcc
     1021 tgggcttcag cccgatccag accggcctgg ccttcatgcc catgaacctg ggcatcgtga
     1081 ccaccgcgat ggtggtcaac acgcggctgc tggcccggac cggcccccgc ccgctgatcc
     1141 ccaccggcat ggtgctggcg tcggtggcga tgctgctgct cacccgcacg ggcgtggact
     1201 cggcgtacgc cagccacgtg ctgccgtcgc tgatcctgct gggcgtgggc ttcggcctga
     1261 cgttcgcgcc ggtgttctcg ggcgcgaccg agggcgtcga gccgcgcgac tccggcatcg
     1321 cctcggccat ggtcaacacc tcgcagcagg tgggcggcgc cgtcggcacc gcgctgctga
     1381 gcacgctggt ggtgtcggcc acgacctcgt tcgccgaggg caaggcgcca tcgccggcgc
     1441 tggccgccga ggcggcggtg cacggctacc acgtggcctt ctgggtctcg gccgggatct
     1501 tcgccctggc cgccgtggtc accgggtcgc tgctgcgctc cggcccgccg ctgtccacgc
     1561 tgcacgcgcg tgagatcgcg gccgccgagg ccgaggaccg cgacgcggtc ctggtggccg
     1621 gttaacccca ctaccgccgc ggggcggggc tgtggttcca tggccgggtg agcgcccggg
     1681 catggaccag cttcgccgcc gtgtcggtgc tgtgggggat cccgtacctg ttcataaagg
     1741 tcgccgtcga ggacggcgtg ccgccggcct tcctcgcctg ggcgcgcgtg ctgctggccg
     1801 cgctggtcct gtgcgcgctg gcgtggcacc ggggcctgtt cgcctcgctc cgggggcgct
     1861 ggcgctgggt cgtgttctac gcggtggtcg agatctcgat cccgttcccg ctgatcgccg
     1921 ccggtgagca gcacgtctcg tcctcgctgg ccgcgatcct gatcgccgcc gtgccgctga
     1981 tcatcgcggt gctggccatc cgcttcgacg ccgacgagcg ggtgcgcggg gcgcgcctga
     2041 tcggcctgct gatgggcttc accggcgtgg tcgccctggt gggcatcgac gtggccggcc
     2101 gctccgacga gctgctgggc gccgtcttca tcctcatcgc cgcggtcggc tacgcctgcg
     2161 gtccgatgac gctgaaccgc aagatgagcg acatcgagcc ggtggccatg atgggcgcct
     2221 cgctggccgt ggcctcggtg gtgctgaccc cgttcgcgtt cgccgacgtc cccgacgcga
     2281 ccccgtcggc cgaggcgctg gcctcggtcg tggtcctggg cctgctgtgc acggccgccg
     2341 cgttcgtcct cttcggcggc ctgttgctcg aggtcggccc tggccgcgca gcggtcatca
     2401 cctacgtcgc cccggtcgtg gccgtgggcc tcggcgtggc cgtgctgggc gagcgcccgg
     2461 gcgtgggtgc cgccgcgggc ctgctgctga tcctcgcggg ctcgtggctg tccaccgacg
     2521 gccggctgcc gccgggcatg ggggcgtggg tggccagggt gcgggcgcgg cgcggcgggc
     2581 ggtcccccga gcgcaccccc gcaccagccg ccccacaagc gcccgagcgc atcgtgggtg
     2641 cgtaggcgcg caccggtacg cacgtgcgcg cccgtgtggt ggcggagccg aagcgggcgc
     2701 gctgatgcat ggccgtcagc gccggcgcgg gggcggcagc ccgcccaggc gaagcgccag
     2761 aatcgccgct agctgcgccg tcaccgcctc cgggtgcgcg cggatccggc ggccggtgaa
     2821 gcggacgatc cggaggttgg ccgcctcgag gtcggcgtcg agcttgtggt cgtcctcgaa
     2881 ggcggcggcc gagccgtgcg tcgagaacgc atcgacctcc acgccgaggc gggcctcggg
     2941 ccagtagagg tcgacctcct ttccggccac caccacgttc gtctgcggtt gtggcagctc
     3001 ggcgcggcgg atcagggcga gca
//
LOCUS       DASUPW010000223         4756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_745091, whole genome shotgun sequence.
ACCESSION   DASUPW010000223 DASUPW010000000
VERSION     DASUPW010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745091"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VFY44_05125"
     CDS             <1..973
                     /locus_tag="VFY44_05125"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEX5909854.1"
                     /translation="HEPDLDAQADDVFGDDLLTLIFTACHPVLSTEARIALTLRLVGA
                     LTTPEIARAFVVPEKTMAQRIVRAKKTLKTAGVSFETPHGDERDERLASVLEVVYAIF
                     NEGYSATAGDDWMRPALTEDALRLGRILAGLEPDEAEVHGLMALMEIQASRAKARTDA
                     DGVPILLLDQDRSRWDQLLIRRGLGALERAEDLAAGRPGPYTLQAAIAACHARAREAE
                     DTDWDRIAFLYANLATRTGSPVVELNRAVAVGMASGPEAGLAVVDQLVAAGDLEGYHL
                     LPSVRGDLLDKLGRGDEARVEFERAAAMTANQRERALLLERAAGTAR"
     gene            complement(918..1601)
                     /locus_tag="VFY44_05130"
     CDS             complement(918..1601)
                     /locus_tag="VFY44_05130"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEX5909855.1"
                     /translation="MATKKRSYGDACGIARALDAVGERWALMVVRELLLGPKRFTDLR
                     TGLPHVSPDVLAQRLKDLEISGVLAKRKLPPPAASQVYELTPKGLALEPVLIALGRWG
                     GSEAAPPTEEMDMSVDAHLLSLRTLFDPEAAGDLKLSVALELDGQSFFATVAGAALVA
                     ERGEAPTPDVTLTGPRSSLIELVRGMRSLDEVLDSGDATLDGDRQGFERFIELFPLPA
                     PAAEPALAG"
     gene            complement(1629..2585)
                     /locus_tag="VFY44_05135"
     CDS             complement(1629..2585)
                     /locus_tag="VFY44_05135"
                     /inference="COORDINATES: protein motif:HMM:NF027219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HEX5909856.1"
                     /translation="MRAFTCGNCGRLAPFESNRCLHCGAEHGFDWGERELVAFWPDER
                     ARCRHRATAACNAVVAAEGDACAACALTRTRPADDDPAGFARFAEAEAAKRRLVFELA
                     ELGLPLDGGLAFDMLSSEREPVTTGHADGVVTIDLAEVDDAHREAVRAQMAEPYRTVL
                     GHLRHEIGHYYQPIVVADWDACRSLFGDEREDYQQAMNRHYEHGAPDDWPQRYVSAYA
                     TMHPWEDWAETFAHYLHIADTVQTALAYGVRVEGPPGLAPMPPDEGEFSTILASWLPL
                     TYALNALNRSMGAEDLYPFVLVPPVIEKLEFVHQCVVGYSKH"
     gene            2609..3322
                     /locus_tag="VFY44_05140"
     CDS             2609..3322
                     /locus_tag="VFY44_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5909857.1"
                     /translation="MPTVQRIDHDAVAVLRLNRPERRNAMDSELLGELLGALEDLARS
                     DHLRALVFSTTSEQALSAGADVSEQLDEAGGVARMEAFTRLYAAVEAFPVPTVAVCVG
                     NVVGAGAELAAGCDLRVGGDNLKLAWAGARHGVPVGPARLVPLVGLSRAKELVYTGRV
                     VSAGEAEEIGFLNRVVPAARAEESALELAGQVAAGSPESLRQLKAMFRSLGGDAERVA
                     YENGLLTEFQRDGAGLPRG"
     gene            complement(3319..>4756)
                     /locus_tag="VFY44_05145"
     CDS             complement(3319..>4756)
                     /locus_tag="VFY44_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEX5909858.1"
                     /translation="RWRSGRGVAVQVRVKKHGRWSRWLDLPNALDHGPDRGAKANGTD
                     PAWIGGARTFQLRTKGRPGGLRAHMVVAGRRGLNRAAVARTTQAGGPTIITRSQWGAA
                     SFKGSPGYGSVQMAYVHHTVSLNDYSKAESPAVVRGIQRYHQSSNGWSDVGYNFLVDR
                     FGQIYEGRAGGIDRAVIGAQAQGWNSVSTGIASVGTHSSTEVSPEAFNAIASLLGWKL
                     SIHGVPVQGTVTLRSAGGASNRFPSGRMVKFQRVSGHRDGCSTSCPGDALYAQLPALR
                     ARAGGQSFETGLSIRLPRQKVAQGKAAAVAGRLVFPDGAPPSGVGLEVQARTAQGWIT
                     MGQTATAPDGSWTTQILVPYSRALRARVPSNGARAELRSPTVRLQVAPTIRAAMAKKD
                     IRRRVRPVVHGRTIPVRKKQKVTVTVERRIGRRWIRVLRITTRARNGRFRLKLPLLRA
                     GRHRVTVTTPTDRLGAAGKSKQLALRVR"
BASE COUNT          606 a         1695 c         1817 g          638 t
ORIGIN      
        1 ccacgagccc gacctggacg cgcaggccga cgacgtcttc ggcgacgacc tgctgacgct
       61 gatcttcacc gcctgccatc cggtcctgag caccgaggcc cggatcgcgc tgacgctgcg
      121 gctggtcggc gcgctgacca cgcccgagat cgcgcgtgcc ttcgtggtgc ccgagaagac
      181 gatggcccag cggatcgtgc gcgcgaagaa gacgctcaag accgccgggg tgtcgttcga
      241 gaccccgcac ggcgacgagc gcgacgagcg cctggcctcg gtgctggagg tggtctacgc
      301 gatcttcaac gagggctact cggccaccgc gggcgacgac tggatgcgcc ccgcgctgac
      361 cgaggacgcg ctccggctgg gccggatcct ggccgggctg gagcccgacg aggccgaggt
      421 gcacgggctg atggcactga tggagatcca ggcctcacgc gccaaggcgc ggaccgatgc
      481 cgacggcgtg ccgatcctgc tgctggacca ggaccgctcg cgctgggacc agctgctgat
      541 ccggcgcggg ctgggggcgc tggagcgcgc ggaggacctg gccgcgggcc ggccgggccc
      601 gtacacgctg caggcggcca tcgccgcctg ccacgcccgc gcccgcgaag ccgaggacac
      661 cgactgggac cggatcgcct tcctgtacgc caacctcgcg acccggaccg ggtcgccggt
      721 ggtggagctg aaccgcgcgg tggcggtggg gatggcctcg gggcccgagg cgggcctggc
      781 ggtggtcgac cagctggtgg cggccggcga cctggagggc taccacctgc tgcccagcgt
      841 ccgcggggac ctgctggaca agctcggccg cggcgacgag gcgcgcgtcg agttcgagcg
      901 cgcggccgcg atgaccgcta accagcgaga gcgggctctg ctgctggagc gggcagcggg
      961 aacagctcga tgaagcgctc gaagccctga cggtcgccat ccagcgtcgc gtcgcctgag
     1021 tccagcacct catcaaggct gcgcatgccg cggaccagct cgatcaggct cgagcgcggg
     1081 ccggtcagcg tcacgtcggg ggtgggggcc tcaccgcgct cggccaccag cgcggcgccg
     1141 gccaccgtgg cgaagaacga ctgcccgtcc agctccaggg ccacgctcag cttcaggtcg
     1201 ccggcggcct cggggtcgaa cagggtgcgc agggacagca ggtgggcgtc cacgctcatg
     1261 tccatctcct cggtgggcgg cgcggcctcg gagccgcccc agcggccgag ggcgatcagc
     1321 accggctcca gggccaggcc cttcggcgtc agctcgtaca cctgcgacgc ggcggggggc
     1381 gggagcttgc gcttggccag cacgccgctg atctccaggt ccttgagccg ctgcgccagc
     1441 acgtcggggc tgacgtgcgg gagcccggtg cgcaggtcgg tgaaccgctt cgggcccagc
     1501 agcagctcgc ggaccaccat cagggcccat cgctcgccta ccgcgtccag cgcccgcgcg
     1561 atcccgcagg cgtcgccgta tgagcgcttc ttcgtagcca tggcgcccat tgtagcgcca
     1621 tttcctactc agtgcttgga ataaccaacc acgcattggt gcacgaactc gagcttctcg
     1681 atcacgggcg ggaccagcac gaacgggtac aggtcctcgg cgcccatgct gcggttgagc
     1741 gcgttcagcg cgtaggtcag cggcagccag ctggccagga tcgtggagaa ctccccctcg
     1801 tcgggcggca tcggggccag gcccggcggg ccctccaccc gcacgccgta ggccagggcg
     1861 gtctgcacgg tgtcggcgat gtgcaggtag tgggcgaacg tctcggccca gtcctcccac
     1921 gggtgcatgg tcgcgtacgc gctgacgtac cgctgcggcc agtcgtccgg cgcgccgtgc
     1981 tcgtagtggc ggttcatcgc ctgctggtag tcctcgcgct cgtcgccgaa cagcgagcgg
     2041 cacgcgtccc agtcggcgac cacgatcggc tggtagtagt ggccgatctc gtgccgcagg
     2101 tggcccagca cggtgcggta gggctcggcc atctgggccc ggacggcctc gcggtgggcg
     2161 tcgtccacct cggccaggtc gatggtcacc accccgtcgg cgtggcccgt ggtcaccggc
     2221 tcgcgctcgc tggagagcat gtcgaaggcc agcccgccgt ccagcggcag ccccagctcg
     2281 gccagctcga acaccagccg ccgcttggcc gcctcggcct cggcgaagcg ggcgaagccg
     2341 gcggggtcgt cgtcggccgg gcgcgtccgg gtcagcgcac aggcggcgca ggcgtcgccc
     2401 tcggcggcca ccaccgcgtt gcacgccgcc gtggcccggt gccggcagcg ggcgcgctcg
     2461 tcgggccaga aggccacaag ctcgcgctcg ccccagtcga aaccgtgctc ggcgccgcag
     2521 tgcaggcagc ggttgctctc gaagggggcc agccggccac agttgccaca ggtaaaggct
     2581 ctcaccggcc aagcgtagga tgccgcgggt gccgaccgtc cagcgcatcg accacgacgc
     2641 cgtcgcggtg ctccgcctga accgccccga gcgtcgcaac gccatggact cggagctgct
     2701 cggcgagctg ctgggggcgc tcgaggacct ggcgcgctcg gaccatctgc gggcgctggt
     2761 gttctccacg acctccgagc aggcgctgtc cgcgggcgcc gacgtgtccg agcagctgga
     2821 cgaggccggc ggcgtggcgc ggatggaggc gttcacccgc ctgtacgcgg cggtcgaggc
     2881 ctttcccgtg cccacggtgg ccgtgtgcgt gggcaacgtg gtgggcgcgg gcgccgagct
     2941 ggccgccggc tgcgacctgc gcgtgggcgg cgacaacctc aagctcgcct gggcgggcgc
     3001 gcgccacggc gtgcccgtcg ggccggcgcg gctggtgccg ctggtgggcc tcagccgggc
     3061 gaaggagctg gtctacaccg gccgcgtggt gtccgccggg gaggccgagg agatcgggtt
     3121 cctgaaccgc gtggtgcccg cggcccgcgc cgaggagtcg gcgctggagc tggccggcca
     3181 ggtggcggcg ggaagcccgg agtcgctgcg ccagctgaag gcgatgttcc gctcgctcgg
     3241 cggcgacgcc gagcgggtcg cctacgagaa cgggttgctc accgagttcc agcgcgacgg
     3301 cgccgggctg ccgcggggct agcgcacccg cagcgccagc tgcttcgact tgccggccgc
     3361 gcccaggcgg tcggtgggcg tggtcaccgt gacccggtgg cggccggcgc gcaggagcgg
     3421 cagcttcagg cggaagcggc cgttcctggc ccgcgtggtg atgcgcagga cgcgaatcca
     3481 gcggcggccg atgcggcgct cgaccgtgac cgtgaccttc tgcttcttgc gcaccgggat
     3541 cgtgcggccg tgcacgacgg ggcggacccg gcggcggatg tccttcttgg ccatggccgc
     3601 gcggatggtg ggtgccacct ggaggcgcac cgtcggcgag cgcagctcgg cccgggcgcc
     3661 gttggagggg acgcgcgccc tgagcgcgcg cgagtagggc accaggatct gggtggtcca
     3721 gctgccgtcg ggggcggtgg cggtctggcc catcgtgatc cagccctggg cggtgcgggc
     3781 ctggacctcg aggccgaccc cgctgggcgg cgcgccgtcg gggaacacca gccgcccggc
     3841 caccgcggcg gccttgccct gcgccacctt ctggcgcggg agccggatgc tcaggccggt
     3901 ctcgaagctc tggcccccgg cccgagcccg cagcgcgggc agttgggcgt acagggcgtc
     3961 gccggggcag ctggtcgagc agccgtcgcg gtggccggag acgcgctgga acttgaccat
     4021 gcggccgctg gggaagcggt tggaggcccc gccggcggag cgcagcgtga ccgtgccctg
     4081 caccggcacg ccgtggatcg agagcttcca gcccagcaac gaggcgatgg cgttgaaggc
     4141 ctcggggctc acctcggtcg agctgtgcgt gcccaccgag gcgatgccgg tggagaccga
     4201 gttccagccc tgggcctggg cgccgatcac ggccctgtcg atgccgccgg cgcggccctc
     4261 gtagatctgg ccgaagcggt cgaccaggaa gttgtagccc acgtcgctcc agccgttgga
     4321 gctctggtgg tagcgctgga tgccgcgcac gaccgcgggc gactcggcct tggagtagtc
     4381 gttcagcgac accgtgtggt gcacgtaggc catctgcacc gagccgtagc cgggcgagcc
     4441 cttgaacgac gccgcccccc actgggagcg ggtgatgatc gtcgggcctc cggcctgggt
     4501 cgtgcgcgcg accgccgcgc ggttgagccc gcgccggccc gccaccacca tgtgggcgcg
     4561 cagcccgccg gggcggccct tggtgcgcaa ctggaaggtc cgcgccccgc cgatccaggc
     4621 cgggtcggtg ccgttggcct tcgcaccccg gtcggggccg tggtccagcg cgttcggcag
     4681 gtccagccag cggctccagc gtccgtgctt cttcacgcgc acctggacgg ccacgccgcg
     4741 gcccgagcgc cagcgg
//
LOCUS       DASUPW010000224         3173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1161391, whole genome shotgun sequence.
ACCESSION   DASUPW010000224 DASUPW010000000
VERSION     DASUPW010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161391"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VFY44_05150"
     CDS             complement(<1..405)
                     /locus_tag="VFY44_05150"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5909859.1"
                     /translation="MAQVGNEELSLHHRRIRERGVNPWVYWPVRMVLKPFLLLFLRMR
                     RTGREHVPTEGPVILAANHRSFLDPFVIGCCVKQPIYFVAKRELFDRRIAGWILNCLG
                     AFPIKRGESDEESMATALALLERGETVMIFPEG"
     gene            449..982
                     /locus_tag="VFY44_05155"
     CDS             449..982
                     /locus_tag="VFY44_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909860.1"
                     /translation="MVDFKRTLNGALAGGVAAATWAAQQPADKRAFASGYDDVELLGK
                     AVTRGESWPAAGLALHVANGAAFGATYSALRPFLPGPLVGRAVSMALIEHVALWPLGR
                     LVDTHHPARKELTTLTGNGRAFAQATWRHFLFGLVLGELERRLNPEGEDEPPEIPVSS
                     NGHGNIELAVGAGSDAS"
     gene            996..1068
                     /locus_tag="VFY44_05160"
     tRNA            996..1068
                     /locus_tag="VFY44_05160"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1029..1031,aa:Thr,seq:tgt)
     gene            complement(1117..1305)
                     /locus_tag="VFY44_05165"
     CDS             complement(1117..1305)
                     /locus_tag="VFY44_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909861.1"
                     /translation="MSIIGLIITIIVAAVVYALCLALGLPSIVGIIAAILVLLAGIPS
                     GGFGLGGRFGGGRAAPPR"
     gene            complement(1385..2071)
                     /gene="sigH"
                     /locus_tag="VFY44_05170"
     CDS             complement(1385..2071)
                     /gene="sigH"
                     /locus_tag="VFY44_05170"
                     /inference="COORDINATES: protein motif:HMM:NF006148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HEX5909862.1"
                     /translation="MAALTSVASVGSPAASRTKAYSQPELEDTYLIALAKQGRADAYN
                     TILKRYHGFVRLKASSYFLIGGDADDLIQEGLVGLYKAIRDYRTDRESSFRNFAELCI
                     TRQIITAVKTSTRNKHTPLNEYVSFSQTPASSGGDGDPTLDELLPGPTVHDPVNQVIS
                     SEELKSLVACLSSVLSELESSVLSLYLDGYSYEVIGERLDCDTKTVDNALQRVKRKVG
                     SHLDSRAVPA"
     gene            complement(2218..2937)
                     /gene="rlmB"
                     /locus_tag="VFY44_05175"
     CDS             complement(2218..2937)
                     /gene="rlmB"
                     /locus_tag="VFY44_05175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX5909863.1"
                     /translation="MILYGRNPVREALRGSRRGVHRVWATEAAQRSEDWLAEAPELHT
                     ADATWIEERAGSADHQGICAEADGYPYADPGRLLAGEQALVVALDEVQDPHNLGAVCR
                     VAESVGAAGVVIPERRAAEVTAAAAKASAGAVEHLPVARVRNLADWLGEAKETGAWVY
                     GAAAGAEAVGYDEPDYRGRVVLVLGSEGKGLRPRVAAACDQLVELPQLGRVGSLNVST
                     AAAALMYGILQFRKVRLDRGP"
     gene            complement(2934..>3173)
                     /locus_tag="VFY44_05180"
     CDS             complement(2934..>3173)
                     /locus_tag="VFY44_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909864.1"
                     /translation="GETLGVGVLEDMLRVLGLENLLDPDAGDGPDAEAERMLAEREQA
                     RAERDFARADELRDRLAAAGWVVRDTPEGARLVRG"
BASE COUNT          449 a         1152 c         1101 g          471 t
ORIGIN      
        1 gccctccggg aagatcatca cggtctcgcc gcgctccagc agggccagcg ccgtggccat
       61 cgactcctcg tcggattcgc cccgcttgat cgggaacgcg ccgaggcagt tcaggatcca
      121 gcccgcgatc cggcggtcga acagctcgcg cttggccacg aagtagatcg gctgcttgac
      181 gcagcagccg atgacgaacg ggtcgaggaa gctgcggtgg ttggcggcca ggatcacggg
      241 gccctcggtg ggcacgtgct cgcggccggt gcggcgcatg cgcaggaaca gcagcaggaa
      301 cggcttgagc accatgcgca cgggccagta gacccacggg ttcacgccgc gctcgcggat
      361 gcggcggtga tgcagcgaca gctcctcgtt cccgacctgg gccatacggt ggattacccg
      421 gacgggggcg gcccgttacg ctgcgtcgat ggtggatttc aagcgcaccc tgaacggggc
      481 tctggcgggc ggggtcgcgg ctgccacctg ggccgcccag caacccgccg acaagcgtgc
      541 cttcgccagc ggctatgacg atgtggagct gctgggcaag gccgtcacgc gaggcgagtc
      601 gtggccggcg gccgggctgg ccctgcacgt ggccaacggc gccgcgttcg gcgccaccta
      661 cagcgccctg cgtcccttcc tgcccggccc gctggtcggc cgcgccgtgt cgatggcgct
      721 gatcgagcac gtcgcgctgt ggccgctggg ccggctggtg gacacccatc accccgcacg
      781 caaggagctg acaacgctga ccggcaacgg tcgcgccttc gcgcaggcca cctggcgcca
      841 cttcctgttc ggcctggtgc tgggcgagct ggagcggcgc ctgaaccccg agggcgagga
      901 cgagccgccg gagatcccgg tgtcctcgaa cggccacggg aacatcgagc tggcggtcgg
      961 cgccggcagc gacgcgtcct agactgagcg ctccggccgg agtagctcag ctggtagagc
     1021 aacgctcttg taaagcgtag gtcgccggtt cgagcccggc ctccggcttc ggcgcgcgcc
     1081 cacgctcgcg cgcctcgccg tcacgtcagc tccgggtcag cggggcggtg cggctctgcc
     1141 gccgccgaag cgtcctccca ggccgaagcc tccactcggg atgcctgcca gcaggaccag
     1201 gatcgcggcg atgatgccga cgatcgacgg aagccccagg gccaggcaca acgcgtagac
     1261 cactgcggcc acgatgatcg tgatgatcaa tccgatgatg ctcattgcac tctcctctca
     1321 gggcccgccg ggtcggcgag cctgagaggg gctaccccgg cgcggatgcg ccaaaccgca
     1381 cgacctacgc cggcaccgcc cgcgagtcca ggtgcgagcc caccttgcgc ttcacgcgct
     1441 ggagcgcgtt gtcgaccgtc ttggtgtcgc agtccaggcg ctcaccgatg acctcgtagg
     1501 agtagccgtc caggtacagg gagaggaccg agctctccag ctcggacaga acgctggaca
     1561 ggcacgccac caggctcttg agctcctcgc tggagatcac ctggttcacg ggatcgtgga
     1621 cggtcggccc cgggagcagc tcgtccaggg tgggatcgcc gtcgccaccg gaggaagccg
     1681 gcgtctgcga gaacgagacg tactcgttca gcggcgtgtg cttgttgcgg gtggaggtct
     1741 tcaccgccgt gatgatctgg cgggtgatgc acagctccgc gaagttgcgg aagctggact
     1801 cacggtcggt gcggtagtcg cggatggcct tgtagaggcc gaccaggccc tcctggatca
     1861 ggtcgtcggc gtcgccgccg atcaggaagt acgagctggc cttcagccgc acgaacccgt
     1921 ggtagcgctt caggatcgtg ttgtacgcgt ccgccctgcc ctgcttggcg agcgcgatga
     1981 ggtacgtatc ctcgagttca ggttgagagt aagccttcgt acgcgatgcc gccggtgacc
     2041 cgacgcttgc aaccgaggtg agtgcagcca cgatgctcct tccagacccc tccctcgggg
     2101 ccctgaagcc atgtgacgcg ttacctcccc tggcgccgcg ttcacgccgt tccggttggg
     2161 tctcgatctc tcgatctcaa cctccagtcc agccttatgg gcgggagtat ctgagagtta
     2221 tggacccctg tcaagccgca ccttgcgaaa ttgcaagatc ccgtacatca gggcggcggc
     2281 ggcggtgctc acgttcagcg atccgacccg cccgagctgg ggcagctcga ccagctggtc
     2341 gcaggcggcc gccacccgcg gcctgagccc cttgccctcc gagcccagga ccagcaccac
     2401 ccggccgcgg tagtccggtt cgtcgtaccc gacggcctcc gcgccggccg cggcgccgta
     2461 gacccatgcg cccgtctcct tggcctcccc cagccagtcg gccaggttcc gcacacgggc
     2521 cacgggcagg tgctcgaccg cgcccgcgga cgccttggcg gcggccgcgg tgacctccgc
     2581 ggcccggcgc tcggggatca cgacccccgc ggcgcccacc gactcggcca cgcggcagac
     2641 cgcgcccagg ttgtgggggt cctgcacctc gtccagcgcg accaccagcg cctgctcgcc
     2701 ggccagcagc cgcccggggt cggcgtacgg gtagccgtcg gcctcggcgc agatgccctg
     2761 gtggtcggcg ctgccggcgc gctcctcgat ccacgtggcg tcggccgtgt gcagctcggg
     2821 cgcctcggcc agccagtcct cggaccgctg ggccgcctcg gtggcccaca cgcggtggac
     2881 gccgcggcgg ctgccgcgca gcgcctcacg gaccgggttg cgcccgtaga ggatcaaccg
     2941 cgcacgagcc gcgcgccctc gggggtgtcg cgcaccaccc agcccgcggc ggccagccgg
     3001 tcgcgcagct cgtcggcgcg ggcgaagtcg cgctcggccc gcgcctgctc gcgctcggcc
     3061 agcatccgct cggcctcggc gtcgggaccg tcgccggcgt cggggtccag caggttctcc
     3121 aggcccagca cgcgcagcat gtcctccagg acgcccacgc ccagcgtctc ccc
//
LOCUS       DASUPW010000225         4674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1022611, whole genome shotgun sequence.
ACCESSION   DASUPW010000225 DASUPW010000000
VERSION     DASUPW010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022611"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..1738)
                     /locus_tag="VFY44_05185"
     CDS             complement(101..1738)
                     /locus_tag="VFY44_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909865.1"
                     /translation="MRGIRGLLTAALAVAALTVCPGAAEAAGWSAPAVAAPDGSPAAV
                     GLDDAGTITVLYFDSDRRPFLASRGPTAGRFGAPVPVGPPGAMAQLAVAPSGRVVVMY
                     SESACPCNATSKLFYRAGPSAATLSAPRLLTGNAHFLPTLSIAANGYAIAAWASSDAV
                     EVATMPPGQGFGAPQQLVKADAATGGGIDFSTSVEAAVNDRGDALVAWDGGKNVQTLD
                     ASYRPAGAAFSPPERLTGEGGYPFTVAMHPAAVGTVRYAARGGAAVRSRGPLGGFTEP
                     APIGGGPFGDLVVSPAGPQAAIWGDQDESRNWRDPGSWRRYVATRRPDGTFAGPRPLT
                     GVVSAEPPAAGLDSLGNLFVAWGEGDLGKGRIRAMTMNRFDPTPADITPPADGGYGVP
                     PGAMAVNAEGGVAEAYGGVPSWVPGGGVGVLVLERKPDQVAPGLAVRPAARRLGRVAV
                     RVRCSEACRARATVNSAARGPRARRSRSTRSRPVTLRRGARGRLTLAVPRSERRRLAR
                     TLRRRGRVKVTLVVRVEDAAGNHRSVRRRLSARRLLR"
     gene            complement(1785..2222)
                     /locus_tag="VFY44_05190"
     CDS             complement(1785..2222)
                     /locus_tag="VFY44_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012117385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEX5909866.1"
                     /translation="MAKVLYTAEATVTGGRAEGHGRTSDGALEVDLRIPADLGGPGGG
                     TNPEQLFAVGYASCFESAMQTVARRLKVEADDVSVDSKVELSPTSEGGFGLSVALDVT
                     LPSIDDAAQAAELVEKAHKVCPYSNATRGNIEVRLTANGQAVG"
     gene            complement(2251..3063)
                     /locus_tag="VFY44_05195"
     CDS             complement(2251..3063)
                     /locus_tag="VFY44_05195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX5909867.1"
                     /translation="MSDGYFTDQSMLRRVHRERAIALSGPRALLMQAAHPLAVTGLLA
                     HSTAMDEPYDRLARTAETMSTIGFGSREEADHVTSHVRQMHTRVKGKIKEPVGAFPAG
                     TPYRADQPDLLLWVLFTLVDSGVVVYRKYVGAMSRAEEAGYWDDYKVVGELFGLERRD
                     MPDTLDDLHEYRREMLEGDVLLVTDWARRRAREIVMEPPIPWVAQPLLQTANFITIGL
                     LPDRIRKEYGFFPLPPAPVRKAVVAGGAEYAKRVLIPFLPDRLRLVPAARAA"
     gene            3100..3837
                     /locus_tag="VFY44_05200"
     CDS             3100..3837
                     /locus_tag="VFY44_05200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HEX5909868.1"
                     /translation="MPAQAYAGKECVCQFRKGLCDQTCVQGMLETPFYIACLKLTGRR
                     CLVVGGGDIGLEKIEGLLACDGSVHVVAPEVCDAVHALVEEGSITWEKREYRLEDLEG
                     TFMVIAATNDSEVNIGVYEDAEKRAMLVNVVDVPPLCNFILPAIVRTGPLAIAISTAG
                     ASPALAKRMKNQIAEEYGDTYARLAIMLNEVRGWAKGTLPTYQDRKVFFESIVNGDPD
                     PIQLLRDGDEDAVRDIIAAAQRTAAPA"
     gene            complement(3842..>4674)
                     /locus_tag="VFY44_05205"
     CDS             complement(3842..>4674)
                     /locus_tag="VFY44_05205"
                     /inference="COORDINATES: protein motif:HMM:NF013810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5909869.1"
                     /translation="LAPAAAAESSLDNPPPGANDFGCKPSAAHPEPVVLVHGLGATMG
                     ENWGYIAPILKARGYCVFALTYGMDPRFRYAGGVIPIEQSAEELRVFVDRVLASTGAA
                     KVDLVGHSEGTFMPQWWLKFLGGAAKTDDYVAMTPLYDGTTLAGLDRLRDALAPFGLS
                     QPVVDGVSRFCASCPQFLRGSETVEKLKAGGKAAPGVDYTTILTRYDELVVPYTSGLL
                     PAPATNHIVQRVCPNDISEHAAVAFDPVVARLILNALDPATARTPSCLGLPPLAPPPA
                     "
BASE COUNT          658 a         1718 c         1709 g          589 t
ORIGIN      
        1 ccgtgggcac gatgcggctg agcgggcgcg acttccgcac cgggaagtac gaggacgagg
       61 tgctgatgga gtacgtggac gaggagcggg cggcctcggc ctagcgcagg aggcgcctgg
      121 cggacagccg gcggcggacg gagcggtggt tgccggccgc gtcctcgacc cgtacgacga
      181 gcgtgacctt gacacgcccg cgccgacgca gcgtgcgcgc gagcctgcgc cgctccgacc
      241 gcgggacggc cagcgtcagc cgccccctcg ccccccgccg gagggtgacc ggacgcgacc
      301 gcgtcgagcg cgaccggcgc gcccgggggc cgcgggcggc gctgttgacg gtagcccggg
      361 cccggcacgc ctcgctgcag cgcacccgca ccgcgacccg gccgaggcgg cgcgcggccg
      421 gacggacggc caggccgggc gcaacctgat cgggcttgcg ctcgagcacc aacacgccca
      481 cccctccgcc cgggacccag gacgggacgc cgccgtaggc ctcggcgacc ccgccctcgg
      541 cgttcaccgc catcgccccc ggagggacgc cgtatccgcc atcggccggc ggggtgatgt
      601 cggccggggt cggatcgaag cggttcatgg tcatggcgcg gatccggccc ttgcccaggt
      661 cgccctcgcc ccaggccacg aagaggttgc ccagcgagtc caggcctgcg gcgggcggtt
      721 cggccgagac cacccccgtc aggggccggg ggccggcgaa cgtcccgtcg ggccggcggg
      781 tggccacgta ccgcctccag ctcccggggt cgcgccagtt ccgggactca tcctggtcgc
      841 cccagatcgc ggcctgcggg cccgccgggc tgaccacgag gtcgccgaac ggcccgcccc
      901 cgatgggagc cggctcggtg aagcccccga gcgggccgcg gctcctgacc gccgcgccgc
      961 cgcgggcggc atagcgcacg gttcccacgg ccgcagggtg catggcgacg gtgaacgggt
     1021 acccgccctc gccggtgaga cgctcgggcg gggagaacgc ggcgcccgcg gggcggtagg
     1081 acgcatccag tgtctgcacg ttcttgccgc cgtcccacgc gaccagcgcg tcgccgcgat
     1141 cgttcacggc agcctcgacc gacgtcgaga agtcgatccc cccgcccgtg gctgcgtcgg
     1201 ccttgaccag ctgctggggc gcgccgaacc cctgacccgg gggcatcgtc gccacctcga
     1261 cggcgtcgga ggaggcccag gcggcgatgg cgtacccgtt ggccgcgatg gacagcgtcg
     1321 gcaggaagtg ggcgttcccc gtcaggaggc ggggcgccga cagcgtcgcg gcgctggggc
     1381 ccgcccggta gaagagcttg ctcgtggcgt tgcaggggca ggcgctctcg gagtacatga
     1441 ccacgacccg cccggacggc gcgaccgcga gctgggccat cgcccccggc ggccccaccg
     1501 gcaccggagc accgaacctg ccggcggtgg gtccgcgcga cgccaggaag gggcgccggt
     1561 cggagtcgaa gtacagcacc gtgatcgtgc ccgcatcgtc gagcccgacc gcggcgggag
     1621 agccatcggg ggccgcgacg gcgggtgccg accaccctgc cgcctcggcg gcacccgggc
     1681 agacggtcaa cgccgcaacc gcgagcgcgg ccgtcaagag ccccctgatt ccccgcacgc
     1741 ggggcagact actcccggat cgaccgggcc gcgaggggga cgcctcagcc gaccgcctgg
     1801 ccgttggcgg tcagcctcac ctcgatgttc ccccgcgtcg cgttcgagta cgggcacacc
     1861 ttgtgcgcct tctcgaccag ctcggcggcc tgggcggcgt cgtcgatgct gggcagcgtc
     1921 acatccagcg ccaccgacag gccgaagcca ccctcggagg tggggctgag ctcgaccttg
     1981 gagtccaccg acacgtcgtc ggcctcgacc ttgagccgcc gcgccacggt ctgcatcgcg
     2041 gactcgaagc acgacgcgta gcccacggcg aacagctgct ccgggttggt gccgccgccc
     2101 gggccgccca ggtcggcggg gatccgcagg tcgacctcga gcgcgccgtc gctggtgcgc
     2161 ccgtgccctt cggcgcggcc gccggtcacg gtcgcctcgg cggtgtacag gaccttcgcc
     2221 atctcgctcc tccggttggg gagacgaaac ctacgccgcg cgcgccgccg gcaccagccg
     2281 cagccggtca ggcaggaacg ggatcagcac gcgcttggcg tactcggcgc cgccggccac
     2341 gacggccttg cgcacgggtg ccggcggcag cgggaagaag ccgtactcct tgcggatccg
     2401 gtcgggcagc aggccgatgg tgatgaagtt ggccgtctgc agcagcggtt gcgcgaccca
     2461 ggggatgggc ggctccatca cgatctcgcg cgcgcggcgg cgggcccagt cggtgaccag
     2521 cagcacgtct ccctccagca tctcgcggcg gtactcgtgc aggtcgtcga gcgtgtcggg
     2581 catgtcacgg cgctccaggc cgaacagctc gccgaccacc ttgtagtcgt cccagtagcc
     2641 ggcctcctcg gcgcgcgaca tggcgccgac gtacttgcgg tagaccacga cgcccgagtc
     2701 caccagcgtg aacagcaccc acagcagcag gtcgggctgg tcggcgcggt agggcgtccc
     2761 ggccgggaag gcgcccaccg gctccttgat cttccccttc acccgcgtgt gcatctggcg
     2821 cacgtgggag gtcacgtggt ccgcctcctc gcggctgccg aacccgatcg tgctcatcgt
     2881 ctcggccgtg cgcgccagcc ggtcgtaggg ctcgtccatg gcggtcgagt gggcgagcag
     2941 cccggtgacc gccagcggat gcgcggcctg catcagcagc gcccgcggcc ccgacagggc
     3001 gatggcgcgc tcgcggtgga cgcgccgcag catcgactgg tccgtgaagt atccgtcgct
     3061 catctgcaac gcaatacggc gcgtagactg gctcggacca tgcccgccca agcgtacgcc
     3121 ggaaaagaat gcgtctgcca gttccgaaag ggcctgtgcg accagacctg tgtccagggg
     3181 atgctggaga cgccgttcta catcgcctgc ctgaagctga ccgggcgccg ctgcctcgtg
     3241 gtcggcgggg gcgacatcgg gctggagaag atcgagggcc tgctggcctg cgacggctcc
     3301 gtgcacgtgg tggcgcccga ggtctgcgac gcggtccacg cgctggtgga ggaggggtcg
     3361 atcacctggg agaagcgcga gtaccggctc gaggacctgg agggcacctt catggtcatc
     3421 gccgcgacca acgacagcga ggtgaacatc ggcgtctacg aggacgccga gaagcgggcg
     3481 atgctggtga acgtggtcga cgtgccgccg ctgtgcaact tcatcctccc cgccatcgtg
     3541 cgcaccggcc cgctggccat cgccatctcc accgcgggcg ccagcccggc gctggccaag
     3601 cggatgaaga accagatcgc cgaggagtac ggcgacacct acgcccgcct ggcgatcatg
     3661 ctcaacgagg tccgcggctg ggccaagggc acgctgccca cctaccagga ccgcaaggtc
     3721 ttcttcgagt cgatcgtcaa cggcgatccg gacccgatcc agctgctccg cgacggcgac
     3781 gaggacgccg tgcgcgacat catcgccgcg gcgcagcgca ccgccgcgcc ggcctgagcg
     3841 cctacgcggg aggcggggcc agcggcggca gccccaggca ggatggcgtc ctcgcggtcg
     3901 ccgggtccag cgcgttcagg atcagccgcg cgaccaccgg gtcgaacgcc acggccgcgt
     3961 gctcggagat gtcgttgggg cacacccgct ggacgatgtg gttcgtcgcg ggcgccggca
     4021 gcaggccact ggtgtacggc accacgagct cgtcgtagcg cgtgaggatg gtcgtgtagt
     4081 ccacgcccgg cgccgccttg ccgccggcct tcagcttctc gacggtctcg gagccgcgca
     4141 ggaactgcgg gcacgaggcg cagaaccgcg acacgccgtc caccaccggc tgcgagagcc
     4201 cgaacggcgc cagcgcgtcc cgcaggcggt ccagcccggc gagggtcgtc ccgtcgtaca
     4261 acggcgtcat ggccacgtag tcgtcggtct tggccgcgcc gcccaggaac ttgagccacc
     4321 actggggcat gaacgtgccc tcggagtggc cgaccaggtc gaccttggcc gcgccggtcg
     4381 acgccaggac gcggtccacg aacacccgca gctcctcggc gctctgctcg atcgggatca
     4441 ccccgccggc gtaccggaag cgcgggtcca tgccgtaggt cagggcgaac acgcagtagc
     4501 cgcgcgcctt caggatcgga gcgatgtagc cccagttctc gcccatcgtg gcgcccaggc
     4561 cgtgcacgag caccaccggc tcggggtggg cggcgctggg cttgcagccg aagtcgttgg
     4621 cgcccggcgg cgggttgtcc aggctggact ccgccgcggc ggcgggcgcc agca
//
LOCUS       DASUPW010000226         6303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1854253, whole genome shotgun sequence.
ACCESSION   DASUPW010000226 DASUPW010000000
VERSION     DASUPW010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1854253"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VFY44_05210"
     CDS             complement(<1..323)
                     /locus_tag="VFY44_05210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5909870.1"
                     /translation="MRVLIDTSYVGRGPSGTAVYVQQLVRALRAEGIEVVEAAQRRRL
                     APGGGNPLRSAANAALDQLWLHGGLARAARAAAADVVHHPLPAHSRRIAVPQVVTVHD
                     VAFAG"
     gene            complement(336..1274)
                     /locus_tag="VFY44_05215"
     CDS             complement(336..1274)
                     /locus_tag="VFY44_05215"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5909871.1"
                     /translation="MRIVVTGAAGFIGSHLSERLLAAGHEVRGLDSFTRFYARAHKEA
                     NLAAAHRAPGFTLYEDTASTPEGLARAFTGADAVCHLAGRPGVRGGAPHAFEEANVRT
                     TESVLSAASAAGARRVILASSSSVYGPAAVPVTEDAPLRPLSHYGRSKLRAERVAGRK
                     AERLGLDLVTLRYFTVYGPRQRPDMAFARYAAAALEDGQMAVLGDGRQTRDFTYVTDV
                     CDATVAALERGRPGATYNVSGGRPATLDEALTLLARSLGAAARLTPAAADGREPRSTA
                     ADLARARDELGWEPAVGLAEGLDHQAAHARATALAV"
     gene            complement(1287..1865)
                     /locus_tag="VFY44_05220"
     CDS             complement(1287..1865)
                     /locus_tag="VFY44_05220"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5909872.1"
                     /translation="MPSRYDDEIWRLVPEDAGPPPAHLAAFARSLDGDGRVLDLGAGD
                     GRLSAELAATRLTLADVSEVALARAAARLPEAAIVALDPDAPLPLDDSAFDLVLCAET
                     LEHVRDVQLLLSEARRVLVPGGTLAVSTPAHGRRTGLAAAALGFERAFDPLSPHLRFF
                     TARSLAGLLDAMGFDPGPVRTQAGTLLARATR"
     gene            1885..3000
                     /locus_tag="VFY44_05225"
     CDS             1885..3000
                     /locus_tag="VFY44_05225"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX5909873.1"
                     /translation="MRVAIDARPLDLPHLRAQGIGRYAQGLLGPLDEVARERGGDLVA
                     LRAPRRGGFGLDAAAGEQRTLRRPPVPARWADLPEQALLPLDLRRARAELVHGLSIYR
                     TAYAPGVPSVTTILDVAPLMWPEHYLRGGRVHRLLYAAARRATLLLAISDAARADVIA
                     HLRVPEERVITVPLAAADHFCRTDARSAIDGPYLLYVGGLANPDWRKNLDGLLEGFAR
                     WQREGDRPEALVMAGGIGPEGERLERRARELGARVVFPGFVADADLPALYGGATCFVT
                     ASRYEGFGLPALEAVSCGTPVVAFDAGAIPEVAGPGALLAPEGDVAALMRLAGSVCDE
                     QPLRDRLRAGGLEHAKRYSWKRTAELTWEAYERCLAS"
     gene            2985..4127
                     /locus_tag="VFY44_05230"
     CDS             2985..4127
                     /locus_tag="VFY44_05230"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5909874.1"
                     /translation="MSRVLIVGAGLTGCTLAHRLAAEGVESVLLERSEVPGGLIRSEH
                     MEGVLYEPHGSHIFHTEDEEVWKLANAMTPFNDYRHRVDINVEGKLLNWPILLSDIDA
                     QSRSEEIHRELEERRDVDAAARAEAGNFEEWCLDLMGPVLYERYIRPYTEKQWGRPAR
                     ELSAQWAPRRVSVRWDNDPYLFPDPFQGWPAGPNGYTDLIDGLLANELVSVRCGTDAT
                     LESIDGLARDERADMVVLTCPLDVFCAERFGALEWRGIHVASVHIPHVDLAQGAMVVN
                     YPGFEYPYIRIHETKHASRQECAGTVLGFEFANAPSRYYPIETERNRSLNDRYQNHLR
                     EQMGSERVVFAGRLANYLYIDMDDCMRQALDAAEGEVLPALGQRVR"
     gene            4127..4918
                     /locus_tag="VFY44_05235"
     CDS             4127..4918
                     /locus_tag="VFY44_05235"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5909875.1"
                     /translation="MAARPAVDVVVPFRGDAADRPRLDGLELREGDTLVVEDNDVTPG
                     YGRNRGAARGSAEWLVFLDADVDPAPDLLDRYFEPEPEAGTALLAGAVEDRAGGSIAA
                     RYAHRRGLMSQENTLAWGRWAFAQTANAAVRRSAIEAVGGFREDIRAAEDADLAYRLK
                     AAGWQMERRDGARAAHVPRASVRALLRQRMAHGAGGAWLDRAYPGSVPAKRWPGFVWW
                     MARYTATGLARAARDRDPDRALVAILEPLDNLAYELGRSRSNERA"
     gene            5100..5978
                     /locus_tag="VFY44_05240"
     CDS             5100..5978
                     /locus_tag="VFY44_05240"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5909876.1"
                     /translation="MQRPSVDVIVPFKGSPAALADLRRRLGELVLAPGDSALVVDNGP
                     EPLPVPDGPVPVHWVGQVPTPAFARNAGAGRGSADWIVFCDADTHAPPGLLDHYFDPP
                     PAERTGLIGGGVLDEPAPANAGVVARYGHLRGVMSQEGTFDFGDRWGYPKTANVACRR
                     AAFEQVGGFTERIRAAEDADLTYRLRAAGWEVERREHASVVHSSRTTLRGLVVQQALW
                     GAGGAWVAERYPGSVPLVGRAGLVRWALSTTLGGFARAVRERSRDAAIMAVMRPVEAL
                     AWEYGRRLLSNEREAR"
     gene            5975..>6303
                     /locus_tag="VFY44_05245"
     CDS             5975..>6303
                     /locus_tag="VFY44_05245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX5909877.1"
                     /translation="MTPRVSLLLPNRDNAPVLDLVLDRLAVNSQYDDLELVVVDDGST
                     DGSREILRRWRDSGRFADFQLIEREPSGVIETLNAGLQAATGELVVQLDGDASVETPG
                     WVGKMVAL"
BASE COUNT          778 a         2358 c         2418 g          749 t
ORIGIN      
        1 atcccggcga aggccacgtc gtgcaccgtg accacctgcg gcaccgcgat ccggcggctg
       61 tgggcgggca gcgggtggtg gaccacgtcg gccgccgcgg cccgggcggc gcgggccagg
      121 cccccgtgca gccacagctg gtccagggcg gcgttggcgg cgctgcgcag cgggttgccg
      181 ccaccgggcg cgagccgcct gcgctgggcc gcctccacca cctcgatccc ctcggcgcgc
      241 agcgcgcgca ccagctgctg cacgtagacc gccgtgcccg acgggccgcg ccccacgtac
      301 gaggtgtcga tcagcacccg caccgggggc ctggctcaga ccgccagggc ggtggcccgg
      361 gcgtgcgcgg cctggtggtc cagcccctcg gccaggccga ccgccggctc ccagcccagc
      421 tcgtcgcggg cccgggccag gtccgcggcg gtggagcggg gctcgcggcc gtccgcggcc
      481 gccggcgtca gccgtgccgc cgcgccgagc gacctggcca gcagggtcag cgcctcgtcc
      541 agcgtggccg ggcggccacc ggagacgttg taggtggcgc ccggccggcc acgctccagc
      601 gcggccacgg tggcgtcgca gacgtcggtc acgtaggtga agtcgcgcgt ctggcggccg
      661 tcgcccagca ccgccatctg cccgtcctcc agcgcggcgg cggcgtaccg ggcgaaggcc
      721 atgtcgggcc gctggcgcgg gccgtacacc gtgaagtagc gcagcgtgac cagatcgagc
      781 cccagccgct cggccttgcg tcccgccacc cgctccgccc gcagcttcga gcggccgtag
      841 tgcgagagcg gccgcagcgg cgcgtcctcg gtcacgggca ccgccgccgg gccgtagacc
      901 gacgaggacg aggccaggat cacacgccgc gcgcccgcgg cggaggccgc gctgagcacg
      961 gactcggtgg tgcgcacgtt ggcctcctcg aacgcgtgcg gcgcgccgcc gcggacgccc
     1021 ggccggcccg ccaggtggca gacggcgtcg gccccggtga acgcccgggc caggccctcg
     1081 ggggtggagg cggtgtcctc gtacagcgtg aagccggggg cgcggtgcgc cgcggccagg
     1141 ttggcctcct tgtgcgcccg cgcgtagaag cgcgtgaacg agtccaggcc acggacctcg
     1201 tggccggcgg ccagcagccg ctcgctcagg tgggagccga tgaacccggc ggctcctgtg
     1261 accacgatcc gcatggagcg tgctttctac cgggtcgcgc gcgccagcag ggtcccggcc
     1321 tgtgtgcgga cgggcccggg gtcgaagccc atcgcgtcca gcaggccggc cagcgaccgg
     1381 gccgtgaaga agcgcaggtg cggcgacagc gggtcgaagg cgcgctcgaa gcccagcgcc
     1441 gcggcggcca ggccggtgcg ccgcccgtgg gcgggcgtgg acaccgccag cgtgccgccc
     1501 ggaaccagga cgcgccgtgc ctccgacagc agcagctgga cgtcgcgcac gtgctccagc
     1561 gtctcggcgc acagcaccag gtcgaacgcg gagtcgtcca ggggcaacgg cgcgtcggga
     1621 tccagcgcca cgatcgccgc ctccggcaac cgcgcggccg cccgggccag ggcgacctcg
     1681 gacacgtcgg ccagcgtcag ccgggtggcg gcgagctccg cgctcaggcg cccgtcgccc
     1741 gcgcccaggt ccagcacgcg cccgtcgccg tccagcgagc gggcgaacgc cgccaggtgg
     1801 gcgggcggcg gcccggcgtc ctcgggcacc agccgccaga tctcgtcgtc gtagcgcgag
     1861 ggcaagcccc gtagcctacg gcgcttgcga gtagccatcg acgcacggcc gctggacctg
     1921 ccgcacctgc gcgcgcaggg catcggccgc tacgcccagg gcctgctcgg gccgctggac
     1981 gaggtggcgc gcgagcgcgg tggcgacctg gtggcactcc gtgcgccccg gcggggcggc
     2041 ttcgggctgg atgccgcggc cggggagcag cgcaccctgc gccgcccgcc ggtcccggcg
     2101 cgctgggcgg acctgcccga gcaggcgctg ctgccgttgg acctccggcg cgcacgcgcc
     2161 gagctggtcc acggcctgtc gatctaccgg accgcgtacg ccccgggcgt gccctcggtg
     2221 accacgatcc tggacgtggc gccgctgatg tggcccgagc actacctgcg tggcggccgg
     2281 gtgcaccggc tcctgtatgc ggcggcacgc cgggcgacgc tgctgctggc catctccgac
     2341 gccgcccgcg cagacgtgat cgcccacctc cgcgtgccgg aggagcgggt gatcacggtg
     2401 ccgctggcgg cggcggacca cttctgtcgc acggacgcgc gctccgcgat cgacggcccc
     2461 tacctgctgt acgtcggcgg gctggccaac cccgactggc gcaagaacct ggacggcctg
     2521 ctcgaggggt tcgcgcgctg gcagcgcgag ggcgaccggc ccgaggcgct ggtcatggcc
     2581 ggcgggatcg ggcccgaggg cgagcgcctg gaacggcgcg cccgggagct gggcgcgcgg
     2641 gtggtgttcc ccggcttcgt cgccgacgcc gatctgcccg cgctgtacgg gggcgccacc
     2701 tgcttcgtga ccgccagccg ctacgagggg ttcggcctgc cggcgctgga ggccgtgtcc
     2761 tgcggcacgc ccgtggtggc cttcgacgcg ggcgccatcc ccgaggtggc cgggcccggg
     2821 gcgctgctcg cccccgaggg cgacgtcgcc gccctgatgc ggctggccgg ctcggtgtgc
     2881 gacgagcagc cgctccgcga tcgcctccgg gccgggggcc tggagcacgc gaagcgctac
     2941 tcctggaagc gcaccgccga actgacctgg gaggcttacg agcgatgtct cgcgtcctga
     3001 tcgtcggagc cggcctgacc ggctgcacgc tggcccaccg gctggccgcc gagggggtcg
     3061 agtccgtgct gctcgagcgc tccgaggtcc cgggcgggct gatccggtcc gagcacatgg
     3121 agggcgtgct ctacgagccc cacggctcgc acatcttcca caccgaggac gaggaggtgt
     3181 ggaagctggc gaacgcgatg acgccgttca acgactaccg ccaccgcgtg gacatcaacg
     3241 tggagggcaa gctcctgaac tggccgatcc tgctgtcgga catcgacgcc cagtcgcgga
     3301 gcgaggagat ccaccgcgag ctggaggagc gccgcgacgt ggacgccgcc gcgcgggcgg
     3361 aggccggcaa cttcgaggag tggtgcctgg acctgatggg gccggtgctc tacgagcgct
     3421 acatccggcc ctacaccgag aagcagtggg gccgcccggc gcgcgagctg tccgcacagt
     3481 gggccccgcg gagggtgtcg gtgcggtggg acaacgaccc ctacctgttc cccgacccct
     3541 tccagggctg gcccgcgggg cccaacggct acaccgacct gatcgacggg ctgctggcca
     3601 acgagctggt gtcggtgcgc tgcggcaccg acgcgacgct ggagtcgatc gacgggctcg
     3661 cgcgcgacga gcgggccgac atggtcgtgc tgacgtgccc gctggacgtg ttctgcgccg
     3721 agcggttcgg cgcgctggaa tggcgcggca tccacgtggc gtcggtgcac atcccgcacg
     3781 tggacctggc ccagggcgcg atggtcgtga actaccccgg cttcgagtac ccgtacatcc
     3841 ggatccacga gaccaagcac gcctcccgcc aggagtgcgc gggcaccgtt ctgggcttcg
     3901 agttcgccaa cgcccccagc cgctactacc ccatcgagac cgagcgcaac aggtcgctga
     3961 acgaccgcta ccagaaccac ctgcgcgagc agatggggag cgagcgggtg gtgttcgcgg
     4021 gccggctggc caactacctc tacatcgaca tggacgactg catgcgccag gccctggacg
     4081 ccgccgaggg cgaggtcctg ccggcgcttg gccaacgcgt gcgctgatgg ccgcccggcc
     4141 ggcggtggac gtggtggtcc cgttccgcgg cgacgcggcc gaccgccctc gcctggacgg
     4201 cctggagctg cgcgagggcg acacgctcgt ggtcgaggac aacgacgtga cgcccggcta
     4261 cgggcgaaac cgcggcgccg cccgcgggag cgccgagtgg ctggtgttcc tggacgccga
     4321 cgtcgacccc gcccccgacc tgctggaccg ctacttcgag ccggagccgg aggcgggcac
     4381 cgcgctgctg gcgggcgcgg tcgaggaccg ggcgggcggc tcgatcgccg cccgctacgc
     4441 gcaccggcgc ggcctgatga gccaggagaa cacgctggcc tggggccggt gggcgttcgc
     4501 ccagaccgcc aacgccgccg tgcgccgcag cgccatcgag gccgtcggcg ggttccgcga
     4561 ggacatccgc gccgccgagg acgccgacct ggcctaccgc ctcaaggcgg cgggctggca
     4621 gatggagcgc cgcgacggcg cccgggcggc ccacgtgccc cgggccagcg tgcgcgccct
     4681 gctgcgccag cgcatggccc acggcgccgg cggcgcctgg ctcgaccgcg cctaccccgg
     4741 ctcggtcccg gcgaagcgct ggccgggctt cgtgtggtgg atggcccgat acacggccac
     4801 cgggctggcc cgcgccgcgc gcgaccgcga ccccgaccgg gcgctggtgg ccatcctcga
     4861 gccgctggac aacctggcct acgagctggg ccgctcgcgc agcaacgagc gggcctgagc
     4921 ccaccgtcat ccggggggac gaatcccggc ccgcgacgga ccgcgctcct cgtgggccgg
     4981 agaacggacc ttggacgcgc acatccgggc ccggaaccgg ccgggcgggc tcggggcccg
     5041 gatgtgtacc cggtcgacgt cgatcaccgg ctggacttgg ccgccctaga ctccccgcgg
     5101 tgcagcggcc gagcgtggac gtgatcgtgc ccttcaaggg cagtcccgcc gccctggcgg
     5161 acctgcgccg gcgcctcggc gagctcgtgc tggcgcccgg cgactcggcg ctggtggtgg
     5221 acaacgggcc ggagccgctg ccggtcccgg acgggccggt gcccgtgcac tgggtcgggc
     5281 aggtgcccac ccccgcgttc gcccggaacg ccggcgcggg ccgcggcagc gccgactgga
     5341 tcgtgttctg cgacgccgac acccacgccc cgcccgggct gcttgaccac tacttcgacc
     5401 cgccaccagc ggagcgcacc gggctgatcg gcggcggcgt cctggacgag ccggctcccg
     5461 ccaatgcggg cgtggtggcg cggtacggcc acctgcgcgg cgtgatgagc caggagggca
     5521 cgttcgactt cggcgaccgc tggggctacc ccaagacggc caacgtggcc tgccgccggg
     5581 ccgcgttcga gcaggtcggc gggttcacgg agcggatccg ggccgcggag gacgccgacc
     5641 tgacctaccg cctgcgcgcc gcgggctggg aggtcgagcg ccgcgagcac gcgtcggtcg
     5701 tgcactccag ccgcaccacc ctgcgcggcc tggtggtgca gcaggcgctg tggggcgccg
     5761 gcggcgcgtg ggtggccgag cgctaccccg ggtccgtgcc gctggtcggc cgcgccggcc
     5821 tggtgcgctg ggcgctgtcc accaccctcg gcgggttcgc ccgcgcggtc cgcgagcgca
     5881 gccgcgacgc ggccatcatg gccgtgatgc gcccggtgga ggcgctggcc tgggagtacg
     5941 ggcgccggct gctctcgaac gagcgggagg cgcgatgacc ccgcgcgtgt cgctgctgct
     6001 gcccaaccgc gacaacgccc cggtgctgga cctggtgctg gaccgcctgg ccgtgaactc
     6061 gcagtacgac gacctggagc tcgtggtcgt ggacgacggc tccaccgacg gcagccggga
     6121 gatcctgcgg cgctggcgcg acagcggccg cttcgccgac ttccagctca tcgagcgcga
     6181 gcccagcggc gtgatcgaga ccctgaacgc gggcctgcag gccgccacag gcgagctggt
     6241 cgtccagctg gacggcgacg cctccgtgga gaccccgggc tgggtgggga agatggtggc
     6301 cct
//
LOCUS       DASUPW010000227         3288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1854313, whole genome shotgun sequence.
ACCESSION   DASUPW010000227 DASUPW010000000
VERSION     DASUPW010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1854313"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /gene="panB"
                     /locus_tag="VFY44_05250"
     CDS             complement(<1..646)
                     /gene="panB"
                     /locus_tag="VFY44_05250"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX5909878.1"
                     /translation="MSARPTAEAPPAKGKPVTLSALAQKKALGEPIAMVTAYDFPSAQ
                     VAEEAGVDVVLVGDSGAMTVLGYPSTVPVTTDEMLMLAAAVRRGLRTPMLVGDLPFGS
                     YEVSDEQAVATAQRFVKEAGCDAVKLERGGVVAERAAAIVAAGIPVMGHVGLTPQTAT
                     ALGGYRSQGRTAERAMQVVEDALALQEAGCFACVFEAVPSELTEAVMPQIEIPVI"
     gene            complement(643..1023)
                     /gene="panD"
                     /locus_tag="VFY44_05255"
     CDS             complement(643..1023)
                     /gene="panD"
                     /locus_tag="VFY44_05255"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEX5909879.1"
                     /translation="MRRTMLKSKIHRATLTGSDLNYVGSITIDPDLLEAADIREHEQV
                     HVVDVDNGARFETYTILGRRGSGDMQVNGAAARLVHTGDKVIVVSYAEYDEAELDAYE
                     PRVVHVNADNDIVTVDDAVSELLS"
     gene            complement(1020..1871)
                     /gene="panC"
                     /locus_tag="VFY44_05260"
     CDS             complement(1020..1871)
                     /gene="panC"
                     /locus_tag="VFY44_05260"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF014611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX5909880.1"
                     /translation="MRTIAELRAALAPERAAGRTVGLVPTMGFFHEGHLSLMRAARAE
                     NDVVVVSLFVNPTQFGPGEDLDSYPRDEQRDADQARAEGVDLLFAPAPEEVYPEGFAT
                     EVRVSGLTDVLDGDPARRGAAHFNGVTTVVCKLLNMVGPDAAYFGQKDAQQALVVTRM
                     ARDLDVPARIEVRPTVREPDGLAMSSRNAYLEPDERERAVALSRALAAADDVVAAGER
                     GSEAVLAAARAELDAAGIAPEYLELRSATDLSPVERVNGSTLLAVAAHVGRARLIDNI
                     VLGGTRT"
     gene            complement(1877..2647)
                     /locus_tag="VFY44_05265"
     CDS             complement(1877..2647)
                     /locus_tag="VFY44_05265"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEX5909881.1"
                     /translation="METIDTDQPFTPQRVSVVGPGRLGRVLTAALGQAGYEVAGPLGR
                     GESPPRADVALLCVPDGQIRSASEAVAGSARMVGHTSGATPLSALAAAGAEQFGIHPL
                     QTFTGAETPRHVLGVGCAIAGSSPAATDVARELALSLGMVPFELSDERRAVYHAAASA
                     ASNFLVTLEAAAERLAGGAGLPPDQARTLLAPLVRSTVENWAELGPERALTGPVARGD
                     EDTVTSQRAAVADTAPELLGLFDVMVDRTRALAAGEAL"
     gene            2770..>3288
                     /locus_tag="VFY44_05270"
     CDS             2770..>3288
                     /locus_tag="VFY44_05270"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX5909882.1"
                     /translation="MTFVTEARSRRLAAMGRPAAERREDLVALYKEARACTRCPLHES
                     RTQVVFGAGNADADLMFVGEAPGATEDQQGIPFVGRAGQLLNELLQEQGMDREDVFIA
                     NVLKSRPPDNRDPQPSEIEACRPYLERQVELIEPRVICTLGNFATKLLTGSPVGITRV
                     HGQPQERTLGDRT"
BASE COUNT          426 a         1194 c         1228 g          440 t
ORIGIN      
        1 cgatcacggg gatctcgatc tgcggcatca ccgcctcggt cagctcggac ggcaccgcct
       61 cgaacacgca ggcgaagcag cccgcctcct gcagagcgag cgcgtcctcg accacctgca
      121 tggcgcgctc cgcggtgcgg ccctgcgagc ggtagccgcc cagcgccgtg gcggtctgcg
      181 gcgtgaggcc gacgtggccc atcaccggga tgccggccgc gacgatcgcc gccgcgcgct
      241 cggcaaccac gccgccgcgc tcgagcttga ccgcgtcaca gccggcctcc ttgacgaacc
      301 gctgcgcggt ggccacggcc tgctcgtcgg agacctcgta ggagccgaag ggcaggtcgc
      361 ccaccagcat cggggtgcgc aggccgcggc gcacggcggc ggccagcatg agcatctcgt
      421 ccgtggtcac gggcaccgtg ctggggtagc ccagcaccgt catggcgccg ctgtcgccga
      481 ccaggaccac gtccacgccg gcctcctcgg ccacctgggc gctggggaag tcgtaggcgg
      541 tgaccatggc gatcggctcg cccagggcct tcttctgggc cagggcggac agggtcacgg
      601 gcttgccctt ggcgggcggg gcctcggctg tgggtcgcgc gctcacgaga gcagctccga
      661 gactgcgtcg tccaccgtga cgatgtcgtt gtcggcgttc acgtgcacga cgcgcggctc
      721 gtaggcgtcc agctcggcct cgtcgtactc cgcgtaggag accacgatga ccttgtcgcc
      781 ggtgtgcacc aggcgcgcgg cggcaccgtt gacctgcatg tcacccgagc cgcgccggcc
      841 caggatcgtg taggtctcga accgcgctcc gttgtccacg tcgaccacgt gtacctgctc
      901 gtgctcgcgg atgtccgcgg cctccagcag gtcggggtcg atggtgatcg atcccacgta
      961 gttcaggtca ctgcccgtga gcgtggcccg gtggatcttg gacttgagca tggtccgtct
     1021 catgtgcgag tccctcccag gacgatgttg tcgatcagtc ttgcccgccc cacgtgggcg
     1081 gctacggcca gcagcgtcga gccgttcacg cgctcgacgg gggaaaggtc ggtggcggag
     1141 cgcagctcca ggtactcggg cgcgatcccg gcggcgtcca gctcggcgcg ggcggcggcc
     1201 agcacggcct cgctcccacg ctctccggcg gccacgacat cgtcggcggc ggccagcgcg
     1261 cggctcaggg ccacggcgcg ctcgcgctcg tcgggttcca ggtaggcgtt gcgcgagctc
     1321 attgccagcc cgtccggctc gcggacggtg gggcggacct cgatccgggc gggcacgtcc
     1381 aggtcgcgcg ccatccgggt gaccaccagc gcctgctggg cgtccttctg gccgaagtag
     1441 gcggcgtcgg ggccgaccat gttcagcagc ttgcagacca cggtcgtcac gccgttgaag
     1501 tgggcggcgc cgcggcgggc ggggtcgccg tccagcacgt cggtcaggcc gctgacgcgg
     1561 acctcggtgg cgaagccctc ggggtagacc tcctcgggcg cgggggcgaa cagcaggtcc
     1621 acgccctcgg cccgggcctg atcggcgtcg cgctgctcgt cgcgcgggta ggagtccagg
     1681 tcctcgcccg ggccgaactg ggtcgggttg acgaacaggg acaccacgac cacgtcgttc
     1741 tcggcccgcg ccgcgcgcat cagcgacagg tggccctcgt ggaagaagcc catggtcggc
     1801 accaggccga cggtgcgccc ggccgcgcgc tcgggggcca gcgcggcccg cagctcggcg
     1861 atggtgcgca cggtcctcac agcgcctccc cggcggccag cgcccgggtg cggtcgacca
     1921 tcacgtcgaa caggcccagc agctcgggcg cggtgtcggc cacggcggcg cgctggctgg
     1981 tcaccgtgtc ctcgtccccg cgggccaccg ggccggtcag cgcccgctcg gggccgagct
     2041 cggcccagtt ctccacggtg ctgcgcacca gcggggccag cagggtccgg gcctggtcgg
     2101 gcggcagccc ggcgccgccc gccagccgct cggcggccgc ctccagggtc accaggaagt
     2161 tggacgccgc cgaggcggcg gcgtggtaga cggcgcggcg ctcgtcggac agctcgaacg
     2221 gcaccatccc caggctcagg gccagctcgc gcgccacgtc ggtcgcggcg gggctggagc
     2281 cggcgatggc gcagcccacc cccagcacgt ggcgcggcgt ctccgcgccg gtgaacgtct
     2341 gcagcgggtg gatgccgaac tgctcggcgc ccgcggcggc cagcgcggac agcggtgtgg
     2401 cgccgctggt gtggccgacc atgcgggccg agcccgcgac ggcctcggag gcggagcgga
     2461 tctggccgtc gggcacgcac agcagcgcca cgtcggcccg gggaggcgac tcgccgcggc
     2521 ccagcgggcc cgcgacctcg tagcccgcct ggcccagcgc ggcggtcagc acccggccga
     2581 ggcggccggg acccaccacg ctgacgcgct ggggcgtgaa cggctgatct gtatcgatcg
     2641 tttccatcgt cctgccggcc cggcggcggg tgcggctggt cgtgtgatgt ggagagctag
     2701 gtccactccc gagacgggta gcggcctgct gtggacagga tactccccgg ggcggcgtac
     2761 gccacaaccg tgacgttcgt cacagaggcg cgctcgcgta ggctggcggc gatgggccgc
     2821 cctgccgccg agcgccgaga ggacctggtc gccctctaca aggaggcgcg cgcgtgcacg
     2881 cgctgcccgc tccacgagtc gcgcacccag gtggtgttcg gggccggaaa cgccgacgcc
     2941 gacctgatgt tcgtgggtga ggcgccggga gccaccgagg accagcaggg catcccgttc
     3001 gtgggccgcg ccggccagct cctgaacgag ctgctccagg agcaggggat ggaccgcgag
     3061 gacgtgttca tcgcgaacgt cctgaagagc aggccgccgg acaaccgcga cccccagccg
     3121 agcgagatcg aggcctgccg cccctacctg gagcgccagg tcgagttgat cgagccgcgc
     3181 gtgatctgca cgctcggcaa cttcgcgacc aagctgctga ccgggtcccc ggtggggatc
     3241 acgcgggtgc acggccagcc gcaggagcgc acgctgggtg accgcacc
//
LOCUS       DASUPW010000228         2110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1299730, whole genome shotgun sequence.
ACCESSION   DASUPW010000228 DASUPW010000000
VERSION     DASUPW010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299730"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VFY44_05275"
     CDS             complement(<1..341)
                     /locus_tag="VFY44_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909883.1"
                     /translation="MRAPGAEQVERVLALRTLGAPQRRLMRGRRGTDVEQAEPEPVPT
                     SRATVIRPEPFSDPSRAEGWLGDLRGSEDEREAEVAAALEVLNRALHAHRTARADPGA
                     RDVTRDSALVVR"
     gene            complement(415..2007)
                     /locus_tag="VFY44_05280"
     CDS             complement(415..2007)
                     /locus_tag="VFY44_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909884.1"
                     /translation="MDSVMLRARLIAGVPADLVGSLTGLTEGRFPVAARARYADDLQE
                     KEAVKRVKRGNAANVVESDDSRDDIEIFAKQGAPVVATNDGEIKKIGVSKKLGRYVTL
                     QDVYGNRYTYSGLGSTAKYYPVPKADASDPKLSARAVKANGDDAKDPKPTASASAGRQ
                     PQKAGKDRPAAKVRLKANQDKDAADEVEPPPAASVPIKERLFAHPDMPAARENGGLDQ
                     LLDAKGTKGFETYANYFSRPFGANAKDVRLRRLKVGSRVIGGTILGRIGGNDEGKASH
                     LTFGIRPAGKGAPKIDPKPILDGWKLLEATAVYRASGRNALYGDESNSLSIGQVLLLP
                     KPLLEKRVLSDNRIEIYQGGEDDIKTGQIDRRVLATLAYLAESGLKPTVTSLKGNHGL
                     LTASGNVSQHSSGNAVDIAKINGIPILGHQEPGGITEQTVRRIMRLQGTMAPDQVISL
                     LELGGPTLAMGDHADHIHVGFRPLFGDNKRLGRQAMAVLKPGQWSDLLKQLRSIDNPV
                     VPTKPSKYALPAKPASDAHRGE"
BASE COUNT          260 a          728 c          777 g          345 t
ORIGIN      
        1 cggaccacca gcgccgagtc ccgcgtcacg tcgcgcgcgc ccgggtccgc gcgggcggtg
       61 cggtgggcgt gcagggcgcg gttgagcacc tccagcgccg ccgccacctc cgcctcgcgc
      121 tcgtcctcgg acccgcgcag gtccccgagc cagccctccg cgcgggatgg gtcggagaag
      181 ggctcaggcc ggatcaccgt ggcccgcgac gtgggcacgg gctcgggctc ggcctgctcg
      241 acgtcggtgc cgcggcgccc gcgcatcagc cggcgctggg gcgcgcccag cgtgcgcagc
      301 gccagcacgc gctctacctg ctcggctccg ggcgcccgca ccaggtagcg gccgtcggcc
      361 gggcccagca ggtagccgaa ctccagctgg acgcagccga agggagccgc tgcgctactc
      421 gccgcgatgg gcgtctgagg ccggcttggc cggcagcgcg tacttcgagg gcttcgtggg
      481 cacgaccggg ttgtcgatcg agcgcagctg cttgagcagg tcggaccact ggcccggctt
      541 gagcacggcc atggcctggc gcccgagcct cttgttgtcc ccgaacagcg gccggaagcc
      601 cacgtggatg tggtcggcgt ggtcacccat ggccagcgtc ggcccgccca gctccagcag
      661 gctgatgacc tggtcggggg ccatggtgcc ctgaaggcgc atgatccggc gcacggtctg
      721 ctcggtgatg ccgcccggct cctggtggcc caggatcggg atgccgttga tcttggcgat
      781 gtcgaccgcg ttgcccgagc tgtgctggga gacgttgccg gaggcggtca gcagcccgtg
      841 gttgcccttg agcgaggtga cggtgggctt gagcccggac tcggccaggt aggccagcgt
      901 ggccagcacg cggcggtcga tctggccggt cttgatgtcg tcctcgccgc cctggtagat
      961 ctcgatccgg ttgtcggaca gcacgcgctt ctccagcagc ggcttgggca gcagcagcac
     1021 ctggccgatc gagaggctgt tcgactcgtc gccgtacagg gcgttgcgac cggaggcgcg
     1081 gtagaccgcg gtggcctcca gcagcttcca gccgtccagg atcggcttgg ggtcgatctt
     1141 cggggcgccc ttgccggcgg ggcggatgcc gaaggtcagg tgcgaggcct tgccctcgtc
     1201 gttgccgccg atgcggccga ggatggtgcc accgatcacg cgcgagccca ccttcaggcg
     1261 gcgcaggcgg acgtccttgg cgttcgcgcc gaaggggcgc gagaagtagt tggcgtaggt
     1321 ctcgaagccc ttggtgccct tggcgtccag cagctggtcc aggccgccgt tctcacgcgc
     1381 cgccggcatg tccgggtgcg cgaacaggcg ctccttgatc ggcaccgagg ccgcgggcgg
     1441 cggctcgacc tcgtcggcgg cgtccttgtc ctggttggcc ttcagccgca ccttcgccgc
     1501 cgggcggtcc ttgccggcct tctgcggctg gcgcccggcc gaggccgagg cggtgggctt
     1561 ggggtccttg gcgtcgtcgc cgttggcctt cacggcgcgc gcggagagct tggggtcgct
     1621 cgcgtcggcc ttgggcacgg ggtagtactt ggcggtgctg cccaggccgg agtaggtgta
     1681 gcggttgccg tacacgtcct gcagggtcac gtagcggccc agcttcttgg agacgccgat
     1741 cttcttgatc tcgccgtcgt tggtggccac cacgggcgcg ccctgcttgg cgaagatctc
     1801 gatgtcgtcg cggctgtcgt cggactcgac cacgttggcg gcgttgccgc gcttgacgcg
     1861 cttgaccgcc tccttctcct ggaggtcgtc ggcgtagcgg gcgcgggcgg cgacggggaa
     1921 gcggccctcg gtcagaccgg tcagcgaccc gaccaagtca gccggcacac cggcgatcag
     1981 ccgggcgcgc aacatcaccg agtccacata ccaatcggca tggttgtagg caaagatcga
     2041 ccgccgcaca tccttctcat aacccgccgc cttcagatac cgccccgcgg caaagatcgc
     2101 atcaacaggg
//
LOCUS       DASUPW010000229         4144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1576781, whole genome shotgun sequence.
ACCESSION   DASUPW010000229 DASUPW010000000
VERSION     DASUPW010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576781"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /gene="rpsD"
                     /locus_tag="VFY44_05285"
     CDS             complement(<1..342)
                     /gene="rpsD"
                     /locus_tag="VFY44_05285"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX5909885.1"
                     /translation="MGRYTGPVERLSRRAGVELELKGERRANRKSGLERRGYAPGEHG
                     RSSGRRRSTYGQQLLAKQTAKRFYGVRERQFRRYVSEAQKSHDALTGDRLLQLLELRL
                     DNVVCRLGFAST"
     gene            complement(410..1363)
                     /gene="holA"
                     /locus_tag="VFY44_05290"
     CDS             complement(410..1363)
                     /gene="holA"
                     /locus_tag="VFY44_05290"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX5909886.1"
                     /translation="MADLKPVYLIHGDDGAKIDEWRTRVRRRAEAEHGPGGLEVFDAK
                     ASTADQLAGTMAALTFATGTRYFLADDVSAWKAADIDPLVQALGGMAPDTVVVLIARA
                     KPLKGLVKAVEAAGGEVREHAAPKPWEMPRWVSARAGEQGARMDSDAAKELVSIVGSG
                     QQRLAREIEKLILAVHPQTRITAEDVEEMAAGETPPKVYDLADAVVGQDLRRALALAE
                     ELALDESPSRLVYPVVRRLREVHRVLALLDAGVPEKDVAGQMKMPPWMAKKAIALART
                     ADRETVERALCRFADLELDLRGGGTLDEHTAVTLAMTRAAA"
     gene            complement(1373..1963)
                     /locus_tag="VFY44_05295"
     CDS             complement(1373..1963)
                     /locus_tag="VFY44_05295"
                     /inference="COORDINATES: protein motif:HMM:NF044515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5909887.1"
                     /translation="MTPPATTAPRRADAVRNRQRVLDAATEAFTELGLDARMEDIARR
                     AGVGVGTVYRHFPTKEALLGELLAEKWRALRDTVQHSLDNEPDPWEAYAGALKRNAEI
                     MAGDAAVRGAVMLSDSPEVWDHAAGAHQELMAVTEVLVERGKAAGVLRADLQVTDIPP
                     MMAGVCATIDVRGPAGQADWRRHLELALDGTRRRAA"
     gene            2060..3523
                     /locus_tag="VFY44_05300"
     CDS             2060..3523
                     /locus_tag="VFY44_05300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5909888.1"
                     /translation="MSADTATDNTKWFALALLAMTQFVIILDASIVNVALPSIGTDLR
                     LSQDDLSWVVNAYTLTFGGFLLLGGRLADLLGRRRMFISGLILFGISSLAGGLAAEEW
                     QLIAARAFQGLGAAIISPAALSIVTTTFTEGKERNTALGVWGAVAGAGGAAGVLMGGV
                     LTEYLGWEWVLWVNVPIALVAAALAPRLLVESRDETATSFDVPGAVSVTAGLAVLVYA
                     LVDAESAGWGSTQTLGLIALALVLLASFIAIESRTAHPLMPFSIFRLRTLRGANIVGL
                     LVGMSLFSMFFFISLYLQQVLGYEPLKAGLAYLPLSILIIISAGVASNLVTRIGFKPT
                     LVTGMLFVSAGLLWFSQVSAPGGSYVSDVLFPSMLAAIGLGMSFVPVTIAAVTGTEPA
                     EAGLASGLINTSQQIGGALGLAVTATVATAATTSAFEGGERVRTVALTEGFQNAFLTC
                     AGFALLGALLAALLISSRDSRDHAEAAQRGDVDPVAA"
     gene            3596..>4144
                     /locus_tag="VFY44_05305"
     CDS             3596..>4144
                     /locus_tag="VFY44_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909889.1"
                     /translation="MPFDPLFKPLQAPGLDLPHRIALGPLTRNRAKGTVPGEMNAEYY
                     GQRATAALTITEGTHPAPEGQGYLDIAGLYTEGHEEGWKLVADRVHEGGGRLWIQVMH
                     CGRVAHPRFTDGRAPVAPSAVKPEGGTWSPEGELGFVTPRALETDELPGLIGDYVQAA
                     ERAVRAGADGIELHAANGYLLHQ"
BASE COUNT          498 a         1545 c         1474 g          627 t
ORIGIN      
        1 ggtggaggcg aagccgagcc ggcagaccac gttgtccagg cgcagctcca gcagctgcag
       61 cagccggtcg ccggtgaggg cgtcgtggga cttctgggcc tcgctgacgt agcggcggaa
      121 ctggcgctcg cgcacgccgt agaagcgctt ggcggtctgc ttggccagca gctgctggcc
      181 gtaggttgac cggcgccggc ccgacgagcg gccgtgttcg ccaggcgcgt agccgcggcg
      241 ttccagcccg ctcttgcggt tcgcgcggcg ctcgcccttc agttccagct ccacgccggc
      301 gcggcgcgag aggcgctcta ccggccccgt gtaacggccc atgcggtcct ttcccgatct
      361 cttagggtca cctaatactt aggtatacct aattcgccgg gaagaggggc tacgccgctg
      421 cgcgggtcat cgccagcgtg acggcggtgt gctcgtccag cgtgccgccg ccgcgcagat
      481 cgagctcgag gtcggcgaag cgacacagcg cccgctccac ggtctcgcgg tcggcggtgc
      541 gggccagggc gatcgccttc ttggccatcc agggcggcat cttcatctgg cccgccacgt
      601 ccttctcggg aaccccggcg tccaggagcg ccaggacgcg gtgcacctcg cgcaggcggc
      661 gcaccaccgg gtagaccagg cggctcgggc tctcgtccag ggccagctcc tcggccagtg
      721 ccagcgcccg ccgcaggtcc tggcccacga cggcgtcggc caggtcgtag accttgggcg
      781 gggtctcccc cgccgccatc tcctcgacgt cctcggcggt gatgcgggtc tgcgggtgca
      841 cggccaggat cagcttctcg atctcccggg ccaggcgctg ctggccgctg cccacgatcg
      901 agacgagctc cttcgccgcg tcggagtcca tgcgcgcacc ctgctcgccc gcgcgtgccg
      961 agacccagcg cggcatctcc cagggcttcg gggccgcgtg ctcgcgcacc tcgccgccgg
     1021 cggcctcgac ggccttgacc agccccttca gcggcttcgc gcgcgcgatc aggaccacga
     1081 ccgtgtcggg ggccatccct cccagggcct ggaccagggg gtcgatgtcg gccgccttcc
     1141 aggcgctgac gtcgtcggcc aggaagtacc gggtgccggt ggcgaaggtc agcgccgcca
     1201 tcgtgccggc cagctggtcg gcggtggacg ccttcgcgtc gaacacctcg agcccgccgg
     1261 ggccgtgctc ggcctcggcg cggcggcgga cgcgggtgcg ccactcgtcg atcttggcgc
     1321 cgtcgtcgcc gtggatcagg tagacgggct tgaggtcggc cacgcggcga tcttaggccg
     1381 cacgacggcg ggtgccgtcc agggccagct ccaggtggcg acgccagtcg gcctggcccg
     1441 ccgggccgcg cacgtcgatg gtcgcgcaga ccccggccat catcggcggg atgtcggtga
     1501 cctggaggtc ggcgcggagc acgcccgcgg ccttcccgcg ctcgaccagc acctcggtga
     1561 ccgccatgag ctcctggtgc gcgccggcgg cgtggtccca cacctcgggc gagtcggaca
     1621 gcatgaccgc gccccgtacc gccgcatccc cggccatgat ctcggcgttg cgcttcagcg
     1681 cgccggcgta ggcctcccac gggtcgggct cgttgtcgag cgagtgctgg acggtgtcac
     1741 gcagcgcacg ccacttctcg gccaggagct cgccgagcag cgcctccttc gtcgggaagt
     1801 ggcggtacac ggtgcccacc cccaccccgg cccggcgcgc gatgtcctcc atccgcgcgt
     1861 ccagcccgag ctcggtgaac gcctcggtcg ccgcgtccag gacccgctgg cggttgcgca
     1921 cggcgtcggc gcggcggggc gcggtggtgg ccggcggcgt catggcccgg atcctagcgg
     1981 taaacggaac caccgttccg catctgctag cttcgcggaa tcgccgttcc gcttacgccg
     2041 ccgacccgct aggagcccca tgtccgccga cacggccacc gacaacacga agtggttcgc
     2101 cctggccctc ctggccatga cccagttcgt gatcatcctg gacgcgtcga tcgtcaacgt
     2161 ggcgctgccc tccatcggca ccgacctccg gctctcccag gacgacctgt cgtgggtcgt
     2221 caacgcctac acgctgacgt tcggcggctt cctgctcctg ggcggccgcc tggccgacct
     2281 gctgggccgg cggcgcatgt tcatctccgg gctgatcctg ttcggcatct cgtcgctggc
     2341 gggcggcctg gccgcggagg agtggcagct gatcgccgcc cgcgcgttcc agggcctcgg
     2401 cgccgcgatc atctcgcccg ccgccctgtc gatcgtgacc accacgttca ccgagggcaa
     2461 ggagcgcaac acggccctgg gcgtctgggg cgcggtggcg ggagccggcg gagcggccgg
     2521 cgtgctgatg ggcggcgtcc tgaccgagta cctgggctgg gagtgggtgc tgtgggtgaa
     2581 cgtgccgatc gcgctcgtgg ccgccgccct ggccccgcgg ctgctggtcg agagccggga
     2641 cgagaccgcg acgagcttcg acgtgcccgg cgcagtgtcg gtcaccgccg gcctggcggt
     2701 gctggtctac gccctggtcg acgccgagtc ggcgggctgg ggctcgaccc agacgctggg
     2761 tctgatcgcc ctggcgctgg tgctgctggc ctcgttcatc gcgatcgagt cgcgtaccgc
     2821 gcacccgctg atgccgttct cgatcttccg cctgcgcacg ctgcggggcg cgaacatcgt
     2881 ggggctgctg gtgggcatgt cgctgttctc gatgttcttc ttcatctcgc tgtacctgca
     2941 gcaggtgctg ggctacgagc cgctcaaggc gggcctggcc tacctgccgc tgagcatcct
     3001 gatcatcatc agcgccggcg tggcctcgaa cctggtcacc cggatcggct tcaagcccac
     3061 gctggtgacc ggcatgctgt tcgtctcggc cggcctgctc tggttcagcc aggtgtccgc
     3121 ccccggcggc agctacgtca gcgacgtgct gttcccctcg atgctggccg ccatcggcct
     3181 ggggatgtcg ttcgtgcccg tgacgatcgc cgcggtcacc ggcaccgagc cggccgaggc
     3241 cgggctggcc tcggggctga tcaacacgtc gcagcagatc ggcggcgccc tgggcctggc
     3301 ggtcacggcc accgtggcca cggccgccac cacgagcgcc ttcgagggcg gcgagcgggt
     3361 ccgcacggtc gcgctgaccg agggtttcca gaacgccttc ctgacgtgcg ccggcttcgc
     3421 gctcctgggc gccctgctgg cggccctgct gatctcgtcg cgcgacagcc gcgaccacgc
     3481 cgaggcggcc cagcgcggcg acgtggatcc cgtcgcggcc tgagcgcgcc gcggcgttcg
     3541 cgccaaccgg cgcaccgctt ggacgcaccg gcgcgcggcg cctaacgtcg cctggatgcc
     3601 cttcgacccg ttgttcaagc ccctccaggc acccggcctc gacctgccgc accggatcgc
     3661 gctgggcccg ctgacgcgca accgcgcgaa gggcaccgtg cccggcgaga tgaacgccga
     3721 gtactacggc cagcgggcca ccgcggcgct gaccatcacc gagggcaccc acccggcccc
     3781 cgaggggcag ggctacctgg acatcgccgg cctgtacacc gagggccacg aagagggctg
     3841 gaagctggtc gccgaccgcg tgcacgaggg cggcgggcgg ctgtggatcc aggtcatgca
     3901 ctgcggccgc gtcgcgcacc cccggttcac cgacggacgc gccccggtgg ctccctccgc
     3961 ggtgaagccg gagggcggca cctggtcgcc cgagggcgag ctcgggttcg tgaccccgcg
     4021 cgccctggag accgacgagc tgccgggcct gatcggcgac tacgtccagg ccgccgaacg
     4081 cgcggtccgc gccggcgccg acggcatcga gctgcacgcc gccaacgggt acctgctgca
     4141 ccag
//
LOCUS       DASUPW010000230         2896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1023884, whole genome shotgun sequence.
ACCESSION   DASUPW010000230 DASUPW010000000
VERSION     DASUPW010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023884"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1154)
                     /locus_tag="VFY44_05310"
     CDS             complement(48..1154)
                     /locus_tag="VFY44_05310"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5909890.1"
                     /translation="MRVCVTGGTGFLGGHVVRELLEAGHEVRVVYRDERRLSRLGALE
                     PEPVKADLFDRAAMRRAVRGCDQLFHVAGFVGSRPPELAWQVNALGPRRAVEAAAAED
                     VGRVVVTSSVAGLGPVAPDAIGTEEDPFRGGALGLVYPDSKHEGELEALGTAARLGIE
                     VVVVNPSYVLGVPVDRSQPGETSTRMIGNYLRGRLPAVVNGRTDFSDVRDVARGHLRA
                     AERGEPGGRYVLGGHDGDWVGLIELVAELSGVHHPVAVLPPMLGAVAERAEALRLPTP
                     IASEGILLMGLNWSYSSRKARGELGYRSRPLEGTVRDTVDWYRELIDAGTLPSSITPL
                     SLGAAGMRVAGRAGLAALARAAGRRTGRTLVTGG"
     gene            1246..2532
                     /locus_tag="VFY44_05315"
     CDS             1246..2532
                     /locus_tag="VFY44_05315"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEX5909891.1"
                     /translation="MRIHPLPRTATALLVAGTAAFCAAASPAAAAPYVPGEVIVKYKD
                     DAVTGGAVGPAPSSHRVAVRGGSSVKATLTRLRRDPRVQYAVPNYKARIAGLTPNDPG
                     FRQQWNLQGGFGINMPDAWDLAKARKAPGGRGVTVAVLDTGVAYQRFGRYRRAPDLSR
                     FVRGYDYVDDDRHPNDENGHGTHVAGTIAQSTGNATGAAGIAYQARIMPVRVLDAEGA
                     GDTFAIARGIRYAVRNKAKIINMSLEFDASVRASQIPDLVSAMRYAKRKGVLIVAAAG
                     NQADSQVAYPARAKDALAVSATTIRGCLAEYSNVGSDVDLTAPGGGRDAVLSDNPYDA
                     TTCKPELAGQFIYQQTFTSSVRNFGLPRGYEGTSMAAPHVSGVAALLVASKRLGARPK
                     PAALTRHLEVTARDLGQPGFDKRYGYGLLDAAAALR"
     gene            complement(2548..>2896)
                     /locus_tag="VFY44_05320"
     CDS             complement(2548..>2896)
                     /locus_tag="VFY44_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5909892.1"
                     /translation="IYLVSAYEPVPEGRLRDERQQVPEDLQWLVNPREDVESTLEGGA
                     ENLAESGLEVETVAREGDPADAILDVAEETGAGLIVVGNKGMTGAKRFLLGSVPNKVS
                     HHAPCSVLIIRTT"
BASE COUNT          399 a         1124 c         1018 g          355 t
ORIGIN      
        1 cagggccagg cgcatgtagt actcgtcgcg agggaagaag gcgtccgtca tccgccggtg
       61 accagggtgc gccccgtgcg ccggcccgcg gcccgggcca gcgcggccag cccggcgcgg
      121 ccggccacgc gcatgcccgc ggcgcccagc gagagcggcg tgatgctgga gggcaacgtc
      181 cccgcgtcga tgagctcgcg gtaccagtcg acggtgtcgc gcacggtccc ctcgagcggg
      241 cggctgcggt agcccagctc gccccgggcc ttgcgcgagg agtagctcca gttcagcccc
      301 atcagcagga tgccctcgga ggcgatcggg gtgggcagcc gcagcgcctc ggcgcgctcg
      361 gccaccgccc cgagcatggg cggcagcacc gccaccgggt ggtggacccc cgacagctcg
      421 gccaccagct cgatcagccc cacccagtcg ccgtcgtggc cgcccagcac gtagcgcccg
      481 ccgggctcgc cgcgctcggc ggcgcgcagg tgcccgcggg ccacgtcgcg cacgtcgctg
      541 aagtcggtgc ggccgttgac cacggccggc agtcgcccgc ggaggtagtt gccgatcatg
      601 cgggtcgagg tctcccccgg ctggctgcgg tcgaccggga cgccgagcac gtaggagggg
      661 ttgaccacca cgacctcgat ccccagccgg gccgcggtgc ccagcgcctc cagctcgccc
      721 tcgtgcttgc tgtcggggta gaccaggccc agcgcgccgc cgcggaaggg gtcctcctcg
      781 gtgccgatgg cgtcgggcgc gaccgggccc agcccggcga cgctggacgt gaccaccacg
      841 cgcccgacgt cctcggccgc cgcggcctcg accgcccggc gcgggcccag cgcgttgacc
      901 tgccacgcca gctcgggcgg gcgcgagccc acgaagccgg ccacgtggaa gagctggtcg
      961 cagccgcgca ccgcccggcg catggcggcg cggtcgaaca ggtcggcctt gacgggctcg
     1021 ggctcgaggg ccccgagacg gctgagccgg cgctcgtcgc ggtagaccac gcgcacctcg
     1081 tggcccgcct cgagcagctc acggaccacg tggccgccca ggaacccggt accacccgtt
     1141 acacaaaccc tcatggaacc ggcatgatcc accgaaggaa gccggacaac ttccttaggt
     1201 ttcgggtgtt ccgggcctcg tacactcggt ctggcaccgt aaaccatgcg catccaccca
     1261 cttccccgca ccgcgaccgc cctgctggtc gccggcaccg ccgccttctg cgcggccgcg
     1321 tcgcctgccg cggcagcgcc gtacgtgccg ggcgaggtca tcgtcaagta caaggacgac
     1381 gcggtcaccg gcggcgcggt cggcccggcg ccgtcctcgc accgggtggc ggtccgtggc
     1441 ggctcgtcgg tcaaggccac gctgacccgg ctgcggcgcg acccgcgcgt gcagtacgcg
     1501 gtgcccaact acaaggcgcg gatcgccggc ctgaccccca acgaccccgg cttccgccag
     1561 cagtggaacc tccagggcgg gttcggcatc aacatgcccg acgcctggga cctggccaag
     1621 gcccgcaagg cgcccggcgg gcggggcgtg accgtggccg tgctggacac cggcgtggcc
     1681 taccagcgct tcggccgcta ccgcagggcg cccgacctga gccggttcgt gcgcggctac
     1741 gactacgtcg acgacgaccg ccatcccaac gacgagaacg gccacggcac ccacgtggcg
     1801 ggcacgatcg cccagagcac cggcaacgcc accggcgccg cgggcatcgc ctaccaggcc
     1861 aggatcatgc cggtgcgggt gctggacgcc gagggcgcgg gcgacacctt cgccatcgcg
     1921 cgcggcatcc gctacgcggt ccgcaacaag gccaagatca tcaacatgag cctggagttc
     1981 gacgcctccg tgcgcgcctc gcagatcccc gacctggtct cggccatgcg ctacgccaag
     2041 cgcaagggcg tgctgatcgt ggccgccgcc ggcaaccagg ccgacagcca ggtggcctac
     2101 ccggcccggg ccaaggacgc gctggccgtg tcggccacca cgatccgcgg ctgcctggcc
     2161 gagtactcga acgtgggctc ggacgtggac ctgaccgctc ccggcggggg ccgcgacgcg
     2221 gtgctctcgg acaacccgta cgacgcgacg acctgcaagc ccgagctggc gggccagttc
     2281 atctaccagc agacgttcac ctccagcgtg cgcaacttcg ggctgccgcg gggctacgag
     2341 ggcacctcga tggccgcgcc gcacgtgtcc ggcgtggccg cgctgctggt ggcctcgaag
     2401 cgcctgggcg cccgccccaa gccggcggcg ctgacccgcc acctcgaggt gaccgcgcgc
     2461 gacctgggcc agcccgggtt cgacaagcgc tacggctacg gcctgctgga cgcagcggcc
     2521 gcgctgcgct gagcttccgc gcgaccgtca ggtcgtccgg atgatcagca cgctgcaggg
     2581 cgcgtggtgc gagaccttgt tgggaaccga gcccagcagg aagcgcttgg cgccggtcat
     2641 gcccttgttg cccacgacga tcagcccggc gccggtctcc tcggccacgt ccaggatggc
     2701 gtcggcgggg tcgccctcgc gggccaccgt ctcgacctcc aggcccgact cggccaggtt
     2761 ctcggcgccg ccctccagcg tcgactccac gtcctcgcgc gggttgacca gccactggag
     2821 gtcctccggc acctgctggc gctcgtcgcg cagccgcccc tcgggcaccg gctcgtaggc
     2881 gctgaccagg tagatc
//
LOCUS       DASUPW010000231         5459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_332479, whole genome shotgun sequence.
ACCESSION   DASUPW010000231 DASUPW010000000
VERSION     DASUPW010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5459
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332479"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VFY44_05325"
     CDS             complement(<1..505)
                     /locus_tag="VFY44_05325"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5909893.1"
                     /translation="MATQVSEKEARQVAEDAREQDWKLPSFGKGLFMGDFQLELIHPQ
                     PGIDPAKKEKGERFLADLKAFLTEKVDPFEIEREAKIPDEVLDGLKELGALGMKVPEE
                     YGGLGLSQVHYNQALMLAGVWHASISTLLSAHQSIGVAEPLMGFGSEEQKREWLPKVA
                     KDHISAFL"
     gene            633..2636
                     /gene="uvrB"
                     /locus_tag="VFY44_05330"
     CDS             633..2636
                     /gene="uvrB"
                     /locus_tag="VFY44_05330"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF003673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX5909894.1"
                     /translation="MPPFKINSAYVPVADQPKARDGLVAGVENGERFQTLLGATGSGK
                     TATMAFTIERLQRPTLVIAHNKTLAAQLCNEFRELFPQNSVEYFVSYYDYYQPEAYVA
                     AQDLYIEKDSSINEEIERLRHAATASLFGRRDTIVVASVSCIFGLGSPEKYDRLMLLL
                     KKGEMIDRDATLRKLVDIQYSRNDTALGRGTFRVRGDTLEVFPAYAETAYRAVLFGDE
                     IEALQEFDPLTGEVLKDVEHVGVWPATHYATDQDTIDRAVSEIRDELEARTKELEAEG
                     KPLESHRLRQRTQFDMEMLRELGFCNGIENYSRILDGRKPGERPYCLVDFFPDDFVCF
                     IDESHQTVPQIGAMYEGDRSRKTTLVEYGFRLPSALDNRPQTFDEFMSITNSLVFDSA
                     TPGPFERDHSSTIVEQIVRPTGVVDPDIDVRETKNQIDDLMNEIKMRTEQGERTLVTT
                     LTKKMSEDLTDYLLEYGVRVRYLHSEVDTLERIQIIRELRLGEFDVLVGVNLLREGLD
                     LPEVSLVAILDADKEGFLRGETSLIQTIGRAARNIKGQVIMYADKETAAMRTAISETD
                     RRREIQVAYNLEHGITPANIQKGVSDIGEFLAQESKVPGRKSRRKSALEDGMSAEEIA
                     RTLVELEEEMLVAAEDLRFEYAAKLRDEIKALRRELDEVEAGA"
     gene            2719..3879
                     /locus_tag="VFY44_05335"
     CDS             2719..3879
                     /locus_tag="VFY44_05335"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX5909895.1"
                     /translation="MALDRQAIERTDFSQARKGYDPDEVDKHLAEIAAQVDELKAQAA
                     EQPESKPATLAGAAAERVRAIVEAAETSAGDIESSSRAEADHILGEARREAEKERADA
                     KAAAEKERTEARDAAQNERSTAKSEAERERSEARSEAERVRAEAAEAARTTREKAESD
                     AASHVATVEAATQQMRSRADTVESDLGGLVDELRTTISSLVESVRSNAGSLESELQGI
                     RAGLAEVREAQPEAPPEPEPAPVVEQAPAEEPAAEEPAAEPVAEEPVAEEETVVDEIP
                     AEETPAEEPVVEEEPEVEVAEELPAEEEPAAEEEPVQEAVEPEPEPEPAPQEPAPSSS
                     GPGDGTEGARLIALNMALNGTPREETSRYLEENFDLEDQGTILDEVYARVGG"
     gene            complement(3881..4399)
                     /locus_tag="VFY44_05340"
     CDS             complement(3881..4399)
                     /locus_tag="VFY44_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX5909896.1"
                     /translation="MGRLIYSAITSLDGYTEDASGDFQWSAPDDEVHRFVNDLTRPVG
                     THLLGRKTHDVMAYWETDDPDWGATEADFASIWRGAEKIVYSRSARRFDPEEVRELKA
                     TRERDLAIGGAELAAQAITAGLVDEYQQFLNPIVVGGGKHFLPQEVRFELELLEQRRF
                     ANGVVFLRYAAV"
     gene            complement(4410..>5459)
                     /locus_tag="VFY44_05345"
     CDS             complement(4410..>5459)
                     /locus_tag="VFY44_05345"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX5909897.1"
                     /translation="RAIEDRTDEEELERAPAPVRRRLITGAFWLTVCGVSLYLVGPTV
                     LDTLDEADRVRGIAPGWLAAMAGLQLLSLACLWELQRIALHTRDMTSVATSQLAGNAL
                     AKVAPGGGAMGAALQYRMLVQSGLPRAGSASALTATNVLVFAVVLALPVLAVPAIAGG
                     AVNHRLIQATLIGVLVFIGLFVVGVAMLGFDRPLLATARLAQRVRNRLRRRSEPARGL
                     PARLVAERDRILTILGRDWKRALAATVGRWAFDDATLLAALAAVGSNPRPSLVLLAFC
                     AAQVLAQIPFTPGGLGFVEAGLTGMLALAGVSAQDALVATFAYRLFSYWLMMPVGLGA
                     LGVHRIALRRRAAAT"
BASE COUNT          902 a         1953 c         1914 g          690 t
ORIGIN      
        1 gcaggaaggc cgagatgtgg tccttggcca ccttgggcag ccactcgcgc ttctgctcct
       61 cggagccgaa gcccatcagc ggctcggcga cgccgatcga ctggtgggcg ctcagcaagg
      121 tggagatgga ggcgtgccac acgccggcca gcatcagcgc ctggttgtag tgcacctgcg
      181 acaggcccag gccgccgtac tcctcgggca ccttcatgcc cagcgcgccc agctccttga
      241 ggccgtccag cacctcgtcg gggatcttgg cctcgcgctc gatctcgaac gggtcgacct
      301 tctcggtcag gaacgccttc aggtcggcca ggaagcgctc gcccttctcc ttcttggccg
      361 ggtcgatgcc gggctggggg tggatcagct ccagctggaa gtcgcccatg aacagcccct
      421 tgccgaagct gggcagcttc cagtcctgct cccgtgcgtc ctcggcgacc tgacgcgctt
      481 ccttctcgct cacctgcgtg gccatgtgtg tggagtccct ccggagatcg tgtccatcgg
      541 gtgtacccgg atcggggcgg cggctcacgc agccgggcgc gccggattgc ggacatcccg
      601 tgaccgcaac cgccgctaac gtcagacggc ccgtgccgcc cttcaagatc aactccgcgt
      661 acgtcccggt agccgaccag ccgaaggccc gcgacggcct cgtggccggg gtcgagaacg
      721 gggagcggtt ccagacgctg ctgggcgcca cgggctcggg caagacggcc acgatggcct
      781 tcacgatcga gaggctgcag cggccgacgc tggtcatcgc ccacaacaag acgctggccg
      841 cgcagctgtg caacgagttc cgggagctgt tcccgcagaa ctcggtcgag tacttcgtca
      901 gctactacga ctactaccag cccgaggcct acgtggcggc gcaggacctg tacatcgaga
      961 aggactcctc gatcaacgag gagatcgagc ggctgcgcca cgccgcgacc gcgtcgctgt
     1021 tcggccgccg cgacaccatc gtcgtggcca gcgtctcctg catcttcggc ctgggctcgc
     1081 ccgagaagta cgaccgcctg atgctgctgc tcaagaaggg cgagatgatc gaccgcgacg
     1141 ccaccctgcg caagctggtg gacatccagt actcgcgcaa cgacaccgcc ctgggccggg
     1201 gcacgttccg ggtgcgcggc gacacgctcg aggtcttccc cgcctacgcc gagaccgcgt
     1261 accgcgccgt gctcttcggc gacgagatcg aggcgcttca ggagttcgac ccgctgaccg
     1321 gcgaggtgct caaggacgtc gagcacgtgg gcgtctggcc ggccacccac tacgccaccg
     1381 accaggacac catcgaccgc gcggtcagcg agatccgcga cgagctggag gcgcggacca
     1441 aggagctgga ggcggagggc aagccgctcg agagccaccg cctgcgccag cgcacccagt
     1501 tcgacatgga gatgctgcgc gagctgggct tctgcaacgg gatcgagaac tactcgcgga
     1561 tcctggacgg gcgcaagccc ggtgagcggc cgtactgcct ggtcgacttc ttcccggacg
     1621 acttcgtctg cttcatcgac gagtcgcacc agaccgtgcc ccagatcggc gccatgtacg
     1681 agggcgaccg ctcgcgcaag acgacgctgg tcgagtacgg gttccgcctg ccgagcgcgc
     1741 tggacaaccg gccccagacc ttcgacgagt tcatgtcgat caccaacagc ctggtcttcg
     1801 actcggccac gcccggcccg ttcgagcgcg accactcgtc gacgatcgtc gagcagatcg
     1861 tgcgcccgac cggcgtggtg gaccccgaca tcgacgtgcg cgagaccaag aaccagatcg
     1921 acgacctgat gaacgagatc aagatgcgca ccgagcaggg cgagcgcacg ctggtgacca
     1981 cgctcacgaa gaagatgtcc gaggacctga ccgactacct gctcgagtac ggcgtgcggg
     2041 tgcgctacct gcactccgag gtggacacgc tggagcggat ccagatcatc cgcgagctac
     2101 gcctgggcga gttcgacgtc ctggtggggg tgaacctgct gcgcgagggc ctggacctgc
     2161 ccgaggtgtc gctggtggcc atcctggacg ccgacaagga gggcttcctg cggggcgaga
     2221 cctcgctgat ccagacgatc ggccgcgcgg cccggaacat caagggccag gtgatcatgt
     2281 acgccgacaa ggagacggcc gcgatgcgga ccgcgatctc cgagaccgac cgccggcgcg
     2341 agatccaggt ggcgtacaac ctcgagcacg gcatcacgcc ggccaacatc cagaagggcg
     2401 tctcggacat cggcgagttc ctggcccagg agtccaaggt gccgggccgc aagtcgcgtc
     2461 gcaagtcggc gctggaggac gggatgtcgg cggaggagat cgcgcggacg ctcgtggagc
     2521 tcgaggagga gatgctggtc gcggccgagg atctgcgctt cgagtatgcg gcgaagctcc
     2581 gcgacgagat caaggccctg cggcgggagc tcgacgaggt cgaagctggc gcctgagggg
     2641 agtcgttccg ccccgatctg ggctactctc ctgcggtcag ggagttgaag tacgaccttt
     2701 tcggcacgaa gggacactgt ggcactggat cggcaggcaa tcgagcggac ggacttctcg
     2761 caggctcgta aggggtatga cccggacgag gtggacaagc acctggcgga gatcgccgcc
     2821 caggtcgacg agctgaaggc gcaggccgcc gagcagcccg agtccaagcc cgcgaccctc
     2881 gccggcgccg ccgccgagcg tgtgcgcgcg atcgtcgagg ccgctgagac cagcgccggc
     2941 gacatcgagt cgagctctcg tgccgaggcc gaccacatcc tgggcgaggc ccgccgcgag
     3001 gccgagaagg agcgcgcgga cgccaaggcc gccgccgaga aggagcgcac cgaggcccgc
     3061 gacgccgccc agaacgagcg ttcgacggcc aagtccgaag ccgagcgcga gcgctccgag
     3121 gccaggtccg aggccgaacg ggtgcgcgcg gaggccgccg aggcggcccg cacgacccgc
     3181 gagaaggccg agtccgacgc cgcgagccac gtggccaccg tcgaggccgc cacccagcag
     3241 atgcgcagcc gggccgacac cgtcgagagc gatctcggcg gcctggtgga cgagctccgc
     3301 accaccatct cctcgctggt cgagagcgtc cggtccaacg ccggctcgct cgagtccgag
     3361 ctacagggca tccgcgccgg gctggccgag gtccgcgaag cccagcccga ggcgccgccc
     3421 gagcccgagc ccgccccggt cgtcgagcag gcgcccgccg aggagcccgc cgccgaggag
     3481 cctgcggccg agccggtcgc cgaggagccc gtggccgagg aggagaccgt cgtcgacgag
     3541 atcccggccg aggagacgcc cgccgaggag cccgtcgtcg aggaggagcc cgaggtcgag
     3601 gtggccgagg agctgcccgc cgaggaggag cccgcggccg aggaggagcc ggtccaggag
     3661 gccgtcgagc cggagcccga gcccgagccc gcgccccagg agcccgcccc gtcgtccagc
     3721 ggacccggtg acggcaccga gggcgcgcgc ctgatcgcgc tgaacatggc gctcaacggc
     3781 acgccgcgtg aggagacctc ccgctacctc gaggagaact tcgacctcga ggaccagggc
     3841 accattctcg acgaggtcta cgcccgcgtg ggcggctgag ctacaccgcc gcgtagcgca
     3901 ggaacaccac gccgttcgcg aagcggcgct gctccagcag ctccagctcg aaccggacct
     3961 cctgcggcag gaagtgcttg ccgcccccga ccacgatcgg gttcaggaac tgctggtact
     4021 cgtccacgag cccggcggtg atcgcctgcg cggccagctc ggccccgccg atcgcgaggt
     4081 cgcgctctcg ggtcgccttc agctcgcgga cctcttccgg gtcgaaccgc cgcgccgacc
     4141 gggagtagac gatcttctcc gcccctcgcc agatggacgc gaagtcggcc tcggtggcgc
     4201 cccagtccgg gtcgtcggtc tcccagtagg ccatgacgtc gtgcgtcttg cgccccagca
     4261 ggtgggtgcc gaccggccgc gtcaggtcgt tcacgaaccg gtgcacctcg tcgtccgggg
     4321 cgctccactg gaagtcgccg gacgcgtcct cggtgtaccc gtccagcgag gtgatcgcgg
     4381 agtagatcag ccggcccacg gcgggaagcc taggtggcgg cggcgcgacg gcgcagcgcg
     4441 atccggtgca cgcccagcgc gcccagcccc acgggcatca tcagccagta cgagaacagc
     4501 cggtaggcga acgtggccac cagcgcatcc tgcgccgaca cgcccgccag ggcgagcatg
     4561 cccgtcaggc ccgcctcgac gaagcccagc ccgccgggcg tgaacgggat ctgggccagc
     4621 acctgcgcgg cgcagaaggc caggaggacc agtgacggcc gcgggttgga gccgacggcg
     4681 gccagcgcgg ccagcagcgt cgcgtcgtcg aacgcccagc ggccgacggt ggccgccagg
     4741 gcgcgtttcc agtcgcgccc caggatggtc aggatccggt cccgctcggc caccaggcgg
     4801 gccggaagcc cgcgcgcggg ctcggaccgg cgccgcagcc ggttgcgcac gcgctgcgcc
     4861 aggcgcgcgg tggccagcag cgggcggtcg aagcccagca tcgcgacccc gaccacgaac
     4921 aggccgatga acaccagcac cccgatcagc gtggcctgga tcagccggtg gttcacggcg
     4981 ccgccggcga tcgcggggac cgccaggacc ggcagggcga gcaccacggc gaacaccagc
     5041 acgttcgtgg cggtcagggc cgacgccgat cccgcccgcg ggagcccgga ctggaccagc
     5101 atccggtact gcagcgcggc tcccatggcg ccgcctccgg gagccacctt ggccaacgcg
     5161 tttccggcca gctgcgaggt ggccaccgac gtcatgtcgc gggtgtgcag ggcgatccgc
     5221 tgcagctccc acaggcaggc cagggacagc agctggaggc cggccatcgc cgccagccag
     5281 cccggcgcga tgccccgcac ccggtccgcc tcgtccagcg tgtccagcac cgtcgggccc
     5341 accaggtaga gcgagacccc gcacaccgtc agccagaacg cgccggtgat caggcgcctg
     5401 cggaccggcg cgggagcccg ctcgagctcc tcctcgtcgg tcctgtcctc gatcgcgcg
//
LOCUS       DASUPW010000232         2508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1300572, whole genome shotgun sequence.
ACCESSION   DASUPW010000232 DASUPW010000000
VERSION     DASUPW010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300572"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..1236
                     /locus_tag="VFY44_05350"
     CDS             199..1236
                     /locus_tag="VFY44_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX5909898.1"
                     /translation="MAFRWFRSGSFIPVNDIAVDLGTANTLVYVKGEGIVLNEPSVVA
                     MEKATNKIKGIGLEAKRMLGRTPEGITAVRPLKDGVIADVDVTEIMLRYFLETVTSKR
                     FLKLKPTVVVGVPSGITELERRAVRQSASAAGAKEVYMVAEPMAAAIGVGLPVETPTG
                     NMVIDIGGGTTEIAVIALSGIVCDTSIRVGGDEIDNAIVTFMRKNYNLMIGEATAEAV
                     KIQIGSAYSTGDEREMDVKGRDLVSGIPKTVRVHSQEIRECIQEPIQAIVEAVRRALE
                     ITPPELASDIVDRGIVMTGGGALIRGLDTLIARETNLPIHVDEDPLTCVVRGAGRILD
                     DVAKYKGVLTS"
     gene            1296..2075
                     /locus_tag="VFY44_05355"
     CDS             1296..2075
                     /locus_tag="VFY44_05355"
                     /inference="COORDINATES: protein motif:HMM:NF018435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX5909899.1"
                     /translation="MAGLFAALSFVVMMLPEVYKDGLGHSLRATVLRPFIAVQRGAAD
                     RQGRYADASSLRAERDSLASFLVGQATMAAENRQLRQLLGFRERLTYSFKAAEARPEA
                     GPGSEGVLRLSAGARDGVRPGAPVITAEAVKIQIGSAYSTGDEREMDVKGRDLVSGIP
                     KTVRVHSQEIRECIQEPIQAIVEAVRRALEITPPELASDIVDRGIVMTGGGALIRGLD
                     TLIARETNLPIHVDEDPLTCVVRGAGRILDDVVKYRGVLTN"
     gene            2144..>2508
                     /locus_tag="VFY44_05360"
     CDS             2144..>2508
                     /locus_tag="VFY44_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909900.1"
                     /translation="MFTALAALVLLMPEPYKDGLGHAVRATVLRPFIAVQRGSADRQG
                     RYADAAALRAERDSLASFLVGQATLAAENRQLRQLLGFRERLTYSFVAAEATPLAGPG
                     SAGVYRISAGRRDGVGDGAP"
BASE COUNT          391 a          840 c          944 g          333 t
ORIGIN      
        1 aaacacccgc ccacccccgg catgccgcct gcatcagcac gcggcggctc ccggcggggc
       61 accggggctt tcgggttgcg tatgtgcgtg gagcggccta gcttgccggc cgttcacaca
      121 cccacgcaac gagagcgggc aggcggttct gcccgaggcg cccggcggcc ccgcagccgg
      181 cgaggaagct cagacgacat ggcgtttcgc tggttccgat cgggcagctt catcccggtc
      241 aacgacattg ccgtagacct gggaaccgcc aacaccctgg tctacgtcaa gggcgagggt
      301 atcgtcctga acgagccctc ggtggtggcc atggagaagg ccaccaacaa gatcaagggg
      361 atcgggctgg aggccaagcg catgctgggc cgcacccccg aggggatcac cgcggtgcgg
      421 cccctgaagg acggggtgat cgccgacgtg gacgtgacgg agatcatgct ccgctacttc
      481 ctggagacgg tgaccagcaa gcggttcctc aagctcaagc ccacggtcgt cgtgggcgtg
      541 cccagcggca tcaccgagct ggagcggcgg gcggtgcggc agagcgcgtc ggcggccggc
      601 gccaaggaag tctacatggt ggccgagccc atggcggccg ccatcggcgt ggggcttccg
      661 gtggagacgc ccacgggcaa catggtcatc gacatcggcg gcgggacgac ggagatcgcc
      721 gtgatcgcgc tgtcggggat cgtgtgcgac accagcatcc gcgtgggggg cgacgagatc
      781 gacaacgcca tcgtcacctt catgcgcaag aactacaacc tgatgatcgg cgaggccacg
      841 gccgaggcgg tcaagatcca gatcggcagc gcgtacagca ccggcgacga gcgcgagatg
      901 gacgtcaagg ggcgcgacct ggtgagcggc attcccaaga cggtgcgcgt gcactcgcag
      961 gagatccgcg agtgcatcca ggagccgatc caggccatcg tcgaggccgt gcgtcgggcg
     1021 ctggagatca ccccgccgga gctggccagc gacatcgtgg accgggggat cgtgatgacc
     1081 ggcggcggcg cgctcatccg cgggcttgac acgctgatcg cgcgcgagac caacctgccg
     1141 atccacgtgg acgaagatcc gctaacctgc gtggtgcggg gtgccgggcg catcctggac
     1201 gacgtggcca aatacaaggg agtgttgacc tcctgagcgc cttcagacct tacctgcgcg
     1261 gcgcgggcga acgcggccgc aagcgcgacc tggcggtggc ggggctgttc gcggccctga
     1321 gctttgtggt gatgatgctg cccgaggtgt acaaggacgg ccttggccat tccctgcgcg
     1381 ccaccgtgct gcggccgttc atcgccgtgc agcgcggcgc ggccgaccgg cagggccgct
     1441 acgccgacgc cagcagcctg cgcgccgagc gcgactcgct ggccagcttt ctggtcggcc
     1501 aggccaccat ggccgcggag aaccggcagc tgcggcagct gctgggcttt cgcgagcggc
     1561 tgacctactc gttcaaggcc gccgaggcgc gccccgaggc ggggcccggc tccgagggcg
     1621 tgctgcgcct gagcgccggc gcgcgcgacg gggtgcgccc cggggcgccg gtgatcacgg
     1681 ccgaggcggt caagatccag atcggcagcg cctacagcac gggcgacgag cgcgagatgg
     1741 acgtcaaggg ccgggacctg gtgagcggca tccccaagac ggtgcgggtg cacagccagg
     1801 agatccgcga gtgcatccag gagccgatcc aggcgatcgt cgaggccgtg cggcgggcgc
     1861 tggagatcac cccgccggag ctggccagcg acatcgtgga ccgggggatc gtgatgacgg
     1921 ggggcggcgc gctgatccgc gggctggaca cgctcatcgc ccgcgagacc aacctgccca
     1981 ttcacgtgga cgaggacccg ttgacctgcg tggtgcgcgg cgccgggcgc atcctggacg
     2041 acgtggtcaa gtaccgagga gtgttgacca actgagcacg ttcagaccct acctgcgcgg
     2101 cgcgggggag cgcggccgca agcgcgacat ggcggtggcg ggggtgttca cggcccttgc
     2161 cgcgctggtg ctgctcatgc ccgagccgta caaggacggg ctggggcacg ccgtgcgcgc
     2221 cacggtgctg cggccgttca tcgccgtaca gcgcggctcg gccgaccgcc aggggcgcta
     2281 cgccgacgcg gccgccctgc gcgccgagcg cgactcgctg gccagctttc tggtggggca
     2341 ggccacgctg gcggccgaga accggcagct gcggcagctg ctgggctttc gcgagcggct
     2401 cacctactcg ttcgtggccg ccgaggccac gcccctggcg gggcccggct cggccggcgt
     2461 gtaccgcatc tccgccggcc gccgcgacgg ggtgggcgac ggggcgcc
//
LOCUS       DASUPW010000233         2756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_885940, whole genome shotgun sequence.
ACCESSION   DASUPW010000233 DASUPW010000000
VERSION     DASUPW010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885940"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VFY44_05365"
     CDS             complement(<1..513)
                     /locus_tag="VFY44_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909901.1"
                     /translation="MRSRSYAALALTLTAAFGVSGCSLGGESDRPPQLGAKSDDKEAA
                     VKLGFPSTATKNTVRVGGADATADAAGVASAVFPATATSNRPTAVVLVDKDDWQGGVT
                     GAVLSSRPIGAPILISDGDELPAVTEDTLKRLKPTGSDLSKDAQVIRIGDKPARPDGF
                     KTAVLEGKDEY"
     gene            630..1907
                     /locus_tag="VFY44_05370"
     CDS             630..1907
                     /locus_tag="VFY44_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5909902.1"
                     /translation="MDFTLNDEQIEFQRKVRRFATEVIRPIADKYDREQSVPWEAIQA
                     AREWGLHGTELLMQMGSDSEGLMGVIYAEEMHYGCAGIALAISGSSLAAAGIAASGTP
                     EQVGKWVPECFGEGDQIKLGAYAVTEAGAGSDVKSLRTTAKLDESTNEWVLNGTKVFI
                     TNGGIADVTVVVATVDPELGHRGQASFIVEKDREGHTQGKKEDKIGIRASHTAEVVLD
                     NVRVPMENLLGGKEKLDKKLEKARSGQKSGPSNALATFEITRPMVGASALGIAQAAYE
                     YTLDYLEDKSDENGPLIEQQHVQQVLADVATEIEAARMLVWRAAWMGRAGKPMTGGQG
                     SMSKLKAGDVTMWATTTLIDLVGPYAQTTDCPLEKYFRDAKIYQLFEGTAQVQRLVIA
                     RMQRSDYRERVAAEAEVRDQAPGNPQGDPVAAG"
     gene            complement(2263..>2756)
                     /locus_tag="VFY44_05375"
     CDS             complement(2263..>2756)
                     /locus_tag="VFY44_05375"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5909903.1"
                     /translation="DRFRSLPIWRGGPLVGALLGDSARYVLSGLVIMVLGLILGFRPD
                     GGVVGVVAAMALVIVFAFGMSWVFTTLGLLLRTPNAVLNAGFMGVFPLIFISNIFVRP
                     STLPDGLREVVEVNPISILTTATRDLMAGNSAGGDIAICLAVAVGLTLVFAPITVRLY
                     GRG"
BASE COUNT          443 a          967 c          984 g          362 t
ORIGIN      
        1 gtactcgtcc ttgccctcga ggaccgcggt cttgaagccg tcgggacggg cgggcttgtc
       61 gccgatgcgg atgacctgtg cgtccttgct caggtccgac ccggtgggct tgaggcgctt
      121 gagcgtgtcc tcggtgacgg cgggcagctc gtcgccgtcg gagatcagga tcggggcgcc
      181 gatcgggcgc gagctgagca ccgcgccggt cacgccgccc tgccagtcgt ccttgtccac
      241 cagcaccacg gcggtgggcc ggttggaggt cgcggtggcg gggaacacgg cgctggccac
      301 gccggcggcg tcggccgtgg cgtcggcgcc gcccacgcgg acggtgttct tggtggccgt
      361 cgacgggaag cccagcttga ccgcggcctc cttgtcgtcg gacttcgcgc ccagctgggg
      421 cggacggtcg gattcgcccc ccaggctgca gccggagacg ccgaaggcgg cggtcagggt
      481 gagggccagg gccgcgtagc tccgagagcg cacgcggcgc actgtaactg gaccggccgc
      541 ccggccacgt cggtgtcgcg gttgactggc cggtcaacta agattgttga ctgacgagtc
      601 aatcgcctta gcccccggga ggcaccgccg tggatttcac cctcaacgac gagcagatcg
      661 agttccagcg caaggtccgt cgtttcgcca ccgaggtcat ccgcccgatc gcggacaagt
      721 acgaccgtga gcagtccgtc ccgtgggagg ccatccaggc cgcgcgcgag tggggcctgc
      781 acggcaccga gctcctgatg cagatgggca gcgactccga gggcctcatg ggcgtcatct
      841 acgccgagga gatgcactac ggctgcgccg gcatcgcgct cgcgatctcc ggctcgtcgc
      901 tggccgccgc cgggatcgcc gcctcgggca cccccgagca ggtcggcaag tgggtccccg
      961 agtgcttcgg cgagggcgac cagatcaagc tgggcgccta cgccgtgacc gaggccggcg
     1021 ccggctccga cgtcaagtcg ctgcgcacca ccgccaagct cgacgagtcc acgaacgagt
     1081 gggtcctgaa cggcaccaag gtcttcatca ccaacggcgg catcgccgac gtgaccgtgg
     1141 tcgtggccac cgtcgatccc gagctgggcc accgcggcca ggcctcgttc atcgtcgaga
     1201 aggaccgcga gggccacacg cagggcaaga aggaggacaa gatcggcatc cgcgcctccc
     1261 acaccgccga ggtggtgctg gacaacgtgc gcgtcccgat ggagaacctc ctgggtggca
     1321 aggagaagct ggacaagaag ctcgagaagg cccgctcggg ccagaagagc ggcccctcca
     1381 acgccctggc caccttcgag atcacgcgcc cgatggtggg cgcctccgcg ctgggcatcg
     1441 cccaggccgc ctacgagtac acgctcgact acctcgagga caagtccgac gagaacggcc
     1501 cgctgatcga gcagcagcac gtgcagcagg tcctggccga cgtggccacc gagatcgagg
     1561 ccgcccgcat gctggtctgg cgcgccgcct ggatgggccg cgccggcaag ccgatgaccg
     1621 gcggccaggg ctcgatgtcc aagctcaagg ccggcgacgt gacgatgtgg gccaccacca
     1681 cgctgatcga cctggtcggc ccctacgcgc agaccaccga ctgcccgctg gagaagtact
     1741 tccgcgacgc gaagatctac cagctgttcg agggcacggc ccaggtccag cggctggtca
     1801 tcgcgcgcat gcagcgctcc gactacaggg agcgcgtcgc ggccgaggcc gaggtccgcg
     1861 accaggcccc gggcaacccc cagggcgacc cggtcgcggc cggctagcca cgtcgcggag
     1921 ttctgccgcg caacctcgcg gcggccttgg cgaacggcgg ctgagtaggg gcacgctgaa
     1981 tcacgagttc tgcccgtcag acgggcagaa cttctcacgc agccggtcgt tcgccccgca
     2041 gcgggccggc cgcggctgac ggggaccgga tccgacccgc ctcgcctggc gcccgtcgtg
     2101 gggccggtca tgctcgtggg cgagcacctg cgcacccgga agcgcgttgt gcgcttgtgg
     2161 gtcgagaggt gttcccccgc gccggagggc ttgcggccag gtcccgccgc cctacccagc
     2221 cggccgttcg ccgacctcgc tcaggcggct ggcggaggtc gcctagcccc tgccgtacag
     2281 ccggacggtg atgggggcga acaccagggt caggcccacc gccaccgcca ggcagatcgc
     2341 gatgtcgccg ccggcggagt tcccggccat caggtcgcgg gtggccgtgg tcaggatcga
     2401 gatcgggttc acctccacga cctccctgag cccgtccggc agggtggagg ggcgcacgaa
     2461 gatgttggag atgaagatca gggggaagac gcccatgaac ccggcgttga gcacggcgtt
     2521 gggggtgcgc agcagcagcc cgagcgtcgt gaagacccag gacatgccga aggcgaacac
     2581 gatcaccagc gccatcgcgg cgaccacgcc gaccacgccg ccgtcgggac ggaagcccag
     2641 gatcagcccg agcaccatga tcaccaggcc cgagagcacg tagcgcgcgc tgtcgccaag
     2701 cagcgcgccc accagcgggc cgccgcgcca gatcggcagt gagcggaacc ggtcga
//
LOCUS       DASUPW010000234         2588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_332692, whole genome shotgun sequence.
ACCESSION   DASUPW010000234 DASUPW010000000
VERSION     DASUPW010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332692"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1832)
                     /locus_tag="VFY44_05380"
     CDS             complement(3..1832)
                     /locus_tag="VFY44_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5909904.1"
                     /translation="MPGAAAAENCPELPIGDCSTVTVPLDRTGAVPGVVKLAVARVPA
                     ARRPSRGTILFLAGGPGESALAGGENLVSLFRRNAPDYDLLTFDQRGTGRSGGLNCSA
                     LAGRGSEASIFGRCGAQLGAKRAFYRTSDSVEDIEAVRVAAGGGQVSILGVSYGGRVA
                     GEYVRRYPAAVSRLVLDSPSTLAGTDPFFLQRQRALPRVLRSICGRACRSFTRSPIGD
                     MTRVADRLAKRSLRTYVVTPRGTRLKVRFAAADLYGLVSLSDLDPVTRARLPGAVAAA
                     RRGDGAPLARALTGTVSALGGPAQQAGPVSDALFAATTCAEAPLPWSPASAPDGGRDR
                     ALSARVRQLGNRPFEPFGARAVIPASFLAQCKRWPAVTPGVRAAPQGPAVPTLVINGT
                     EDLRTPVEEGRELAAAYPGGRALAVPAAGHSAITTDTTRCAPRAGFAFLAGAPAPAAC
                     PRAAREVATAGRPPLSLRGVGGRSRKAKTVRAVRLTAQDMLVSLQGTSTARFGGLRGG
                     YATAGLRPPRVRLTGYQFVPGVKVSGVLNRRRGRLVGTLTVFGGGSVPASVKVSRSGR
                     FDARFAGEPTVITSASSTGEPLRVPARPRRPALPGPLVPDALR"
     gene            complement(1927..>2588)
                     /locus_tag="VFY44_05385"
     CDS             complement(1927..>2588)
                     /locus_tag="VFY44_05385"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5909905.1"
                     /translation="AVLHERAAMPVPDGIDWPAAGGLAEVFVTAHDAVFTQAGLRPGE
                     RLLVHGAAGGVGTAAIQMARAAGASVTATVRNADLRDRVAELGAEVIDPEGFADHGPF
                     DVVLELVGGPNLPDDVKAIATGGRIVVIGVGGGPKAELNLLALMGKRGRISASTLRAR
                     PLEEKAAAMRRVEAELLPLFASGALEVPVAATYELDRAEEAYDQFAAGGKFGKIVLTT
                     G"
BASE COUNT          317 a         1005 c         1008 g          258 t
ORIGIN      
        1 cgtcagcgga gcgcgtccgg cacgagcggc cccggcagcg ccgggcgccg cggccgcgcg
       61 ggcacgcgca gcggctctcc ggtggacgac gccgaggtga tcaccgtggg ctcgcccgcg
      121 aaccgggcgt cgaagcggcc cgaccggctg accttcacgc tcgccggcac cgagccgccc
      181 ccgaacacgg tgagcgtccc gaccaggcgg ccgcgcctgc ggttgagcac gcccgagacc
      241 ttcacgcccg ggacgaactg gtagccggtc agccgcacgc ggggcgggcg caggccggcg
      301 gtggcgtagc ccccgcgcag gccgccgaag cgcgccgtcg aggtgccctg gagggagacc
      361 agcatgtcct gagcggtcag gcgcacggcg cgcaccgtct tggccttgcg cgaccggccc
      421 cccacgccgc gcagcgacag cggcgggcgc ccggccgtgg ccacctcgcg ggcggcgcgc
      481 gggcaggcgg cgggcgcggg cgcgccggcc aggaaggcga agccggcccg gggggcgcag
      541 cgggtggtgt cggtggtgat ggccgagtgc cccgcggcgg gcacggccag cgcgcgcccg
      601 cccgggtacg cggccgccag ctcgcggccc tcctcgaccg gcgtgcgcag gtcctcggtg
      661 ccgttgatga ccagcgtcgg gaccgcgggc ccctgcgggg cggcgcgcac gccgggcgtg
      721 accgccggcc agcgcttgca ctgggccagg aagctggccg ggatcacggc gcgggcgccg
      781 aagggctcga acggcctgtt gcccagctga cgcacgcggg cggacagcgc ccggtcgcgg
      841 cccccgtcgg gcgcggacgc cgggctccag ggcagcggcg cctcggcgca ggtggtggcg
      901 gcgaacaggg cgtcgctgac cggcccggcc tgctgggccg gcccgcccag cgcggacacg
      961 gtgccggtga gcgcccgggc cagcggagcg ccgtcgcccc gccgggcggc ggccacggcg
     1021 ccgggcagcc gggcgcgggt gaccgggtcc aggtcgctca gcgacaccag cccgtacagg
     1081 tcggcggccg cgaagcgcac cttcagccgc gtgccccgcg gggtcacgac gtacgtgcgc
     1141 agggaccgct tggccagccg gtccgccacc cgcgtcatgt caccgatcgg cgagcgcgtg
     1201 aacgagcggc aggcccgccc gcagatggac cgcagcacgc gcgggagcgc gcgctggcgc
     1261 tgcaggaaga agggatcggt gccggccagc gtgctgggtg agtccaggac cagcctggag
     1321 accgcagccg ggtagcggcg cacgtactcg cccgccacgc gcccgccgta ggagacgccg
     1381 aggatggaga cctgtccgcc accggcggcg acacgcaccg cctcgatgtc ctcgaccgag
     1441 tcgctggtgc ggtagaaggc gcgcttggcg cccagctgcg ctccgcagcg cccgaagatg
     1501 ctggcctcgc tgccgcgccc ggcgagcgcg ctgcagttga gcccgcccga gcggcccgtg
     1561 ccgcgctggt cgaaggtcag caggtcgtag tcgggcgcgt tgcgccggaa caggctgacc
     1621 aggttctcgc cgccggccag ggcggactcg ccggggccgc cggccaggaa gaggatggtg
     1681 ccgcgggagg gccggcgggc ggccggcacg cgcgccacgg ccagcttcac cacgcccggc
     1741 acggcgcccg tccggtccag cggcacggtg accgtgctgc agtcgccgat cgggagctcc
     1801 gggcagttct cggcggccgc cgcgcccggc atcgacccga gcacggcccc agcggccgcc
     1861 agggccaccg cgagtcgcgc gaaccggtgc ggtcgtcgcc cccagatacc ccgtatctgc
     1921 ccggattcag ccagtggtca acacgatctt gccgaacttg ccgccggcgg cgaactggtc
     1981 gtaggcctcc tcggcccggt ccagctcgta cgtggccgcc accgggacct ccagggcgcc
     2041 cgaggcgaac agcggcagca actcggcctc gacccggcgc atcgcggccg ccttctcctc
     2101 cagcggccgg gcccgcagcg tggacgcgct gatgcgcccg cgcttgccca tcagggccag
     2161 caggttcagc tcggccttgg ggccgccgcc cacgccgatg accacgatcc ggcccccggt
     2221 ggcgatggcc ttgacgtcgt cgggcaggtt cggcccgccg accagctcca ggaccacgtc
     2281 gaacgggccg tggtcggcga agccctcggg atcgatgacc tcggcgccca gctcggccac
     2341 ccggtcgcgc aggtcggcgt tgcgcaccgt ggcggtcacc gaggcgccgg ccgcgcgcgc
     2401 catctggatc gcggcggtgc ccacgccgcc ggcggcgccg tgcaccagca gccgctcgcc
     2461 cggccgcagg ccggcctggg tgaagaccgc gtcgtgcgcg gtcacgaaga cctcggccag
     2521 cccgccggcc gccggccagt cgatcccgtc gggcaccggc atggccgcgc gctcgtgcag
     2581 cacggcca
//
LOCUS       DASUPW010000235         2044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_54462, whole genome shotgun sequence.
ACCESSION   DASUPW010000235 DASUPW010000000
VERSION     DASUPW010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54462"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..655)
                     /locus_tag="VFY44_05390"
     CDS             complement(47..655)
                     /locus_tag="VFY44_05390"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX5909906.1"
                     /translation="MAWREEVAREKRAAFAGQEYWGRPVPGFGDLGARIYILGLAPAA
                     HGANRTGRVFTGDRSGDFLFAALFRAGLANQPQSFAIDDGLELTGAFVAAAVRCAPPA
                     NKPTPDERDRCAPYAERELELIGPRVVLCLGAFAWDAALRLTGPPPKPKPKFGHGAEH
                     AVPGGPVMVGCFHVSQQNTFTGKLTEPMIDAVFERAKELAAR"
     gene            complement(694..1515)
                     /locus_tag="VFY44_05395"
     CDS             complement(694..1515)
                     /locus_tag="VFY44_05395"
                     /inference="COORDINATES: protein motif:HMM:NF011648.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5909907.1"
                     /translation="MATRLIGRPKQEQTVSGGSMTGFQGMRWLARRETLRVTKIWTQT
                     ILAPVVSSLLFILVFGLSLSGRVRNVSGVPYEEFIVPGLIAMAMVQAAFANNSSTLFQ
                     ARFDRYINDILSSPMHPWQMTAGFVIGGIYRGFLIGAALLVLAVILTGVGIAHPFALA
                     AVVVLCLVLFAAFGTVVGVFAQSWDSSSFIANVVILPLAFLGGTFYSVSLLPTPWEEL
                     SHINPIFYIVQAMRFGLLGESDVSIWLSLGVVLALTIPAYMWAQWLFTSGRKLKA"
     gene            complement(1515..>2044)
                     /locus_tag="VFY44_05400"
     CDS             complement(1515..>2044)
                     /locus_tag="VFY44_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5909908.1"
                     /translation="RSLLARSWIGLAEQDINLDRFLTVRETLIYHGGYFGFPTDEAGE
                     RADEMIDAFDLRAKRDVRTTKLSGGQRRRLLLARALMHRPRLVILDEPTAGVDFELRQ
                     ELWAYIRQLHAEGTTIVLTTHYLEEAEELCEEIALIRGGKLIARDTADGLRESYGADT
                     LSGVYVKAMHEAAAV"
BASE COUNT          319 a          713 c          742 g          270 t
ORIGIN      
        1 ccacccctcc gtcgacggct acaggcggct ggccgccgac gtgcgcctac cgcgccgcta
       61 gctccttcgc gcgctcgaac acggcgtcga tcatcggctc ggtcagcttg cccgtgaacg
      121 tgttctgctg gctgacgtgg aagcagccga ccatgaccgg gccaccgggc acggcgtgct
      181 cggcgccgtg gccgaacttc ggcttgggct tggggggagg gccggtcagc cgcagggcgg
      241 cgtcccacgc gaaggcgccc aggcacagga ccacccgggg gccgatcagc tccagctccc
      301 gctcggcgta gggcgcgcag cggtcgcgct cgtccggggt gggcttgttg gcgggcgggg
      361 cgcagcggac ggccgcggcc acgaacgcgc cggtcagctc cagcccgtcg tcgatggcga
      421 acgactgggg ctggttggcc agcccggcgc ggaacagcgc ggcgaacagg aagtcgcccg
      481 agcggtcgcc ggtgaacacc cgcccggtgc ggttggcccc gtgggccgcg ggcgccaggc
      541 ccaggatgta gatgcgcgcg cccaggtcgc cgaagccggg gaccggccgg ccccagtact
      601 cctggccggc gaacgccgcg cgcttctcgc gcgcgacctc ctcgcgccac gccaccagcc
      661 gcgggcaggc gcggcaggcg gtgatctcgg ccgctaggcc ttcagcttgc gaccgctggt
      721 gaacagccac tgggcccaca tgtacgccgg gatggtcagg gcgagcacga cgcccagcga
      781 cagccagatg ctcacgtcgc tctcgcccag caggccgaag cgcatggcct ggacgatgta
      841 gaagatcggg ttgatgtggc tgagctcctc ccacggggtg ggcagcagcg acaccgagta
      901 gaaggtgccg cccaggaagg ccagcggcag gatcaccacg ttggcgatga agctcgagct
      961 gtcccagctc tgggcgaaca cgccgaccac ggtgccgaag gcggcgaaca gcaccaggca
     1021 cagcaccacc acggcggcca gggcgaacgg gtgggcgatg ccgacgccgg tcaggatcac
     1081 ggccagcacc agcagcgccg cgccgatcag gaagccgcgg tagatgccgc cgatcacgaa
     1141 gcccgcggtc atctgccacg gatgcatggg cgaggacagg atgtcgttga tgtaacggtc
     1201 gaaccgggcc tggaacagcg tcgacgagtt gttggcgaac gcggcctgca ccatggccat
     1261 ggcgatcagg ccgggcacga tgaactcctc gtagggcacg ccggacacgt tgcgcacgcg
     1321 gccgctcagc gacagcccga acaccaggat gaacagcagc gagctgacca ccggggccag
     1381 gatcgtctgg gtccagatct tggtcacgcg cagggtctcg cgccgtgcca gccagcgcat
     1441 gccctggaag cccgtcatgg agccacccga gacggtctgc tcctgcttgg gcctgccgat
     1501 cagcctggtg gccatcagac cgcagccgcc tcgtgcatgg ccttcacgta cacgccggag
     1561 agggtgtcgg cgccgtagga ctcgcgcagg ccgtcggcgg tgtcgcgcgc gatcagcttg
     1621 ccgccgcgga tcagggcgat ctcctcgcac agctcctcgg cctcctcgag gtagtgcgtg
     1681 gtcagcacga tcgtggtgcc ctcggcgtgc agctggcgga tgtaggccca cagctcctgg
     1741 cgcagctcga agtccacgcc ggcggtcggc tcgtccagga tcaccaggcg cgggcggtgc
     1801 atcagcgcgc gcgccagcag cagccggcgg cgctggccgc cggacagctt ggtggtgcgc
     1861 acgtcgcgct tggcgcgcag gtcgaacgcg tcgatcatct cgtcggcgcg ctcgcccgcc
     1921 tcgtcggtgg ggaagccgaa gtagccgccg tggtagatca gcgtctcgcg cacggtcagg
     1981 aaccggtcca ggttgatgtc ctgctcggcc agcccgatcc agctccgtgc gagcagcgac
     2041 cgcg
//
LOCUS       DASUPW010000236         4292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_746819, whole genome shotgun sequence.
ACCESSION   DASUPW010000236 DASUPW010000000
VERSION     DASUPW010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746819"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="VFY44_05405"
     CDS             <1..1082
                     /locus_tag="VFY44_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909909.1"
                     /translation="VVPRTLLIALVMLVALPGVAQAAVSTGDEPEPANGLTPPTATQD
                     PSISGSATVGQSLTANLGTWDSPPLTSVDLDHIWLRCSASGTGCAQVAGATPSYALTG
                     ADVGRIIKLRVRGTTQGLGSGYRERDATGVVISAAPVFPVAPSNVTPPTITGTAREGE
                     VLTLNTGTWTGSDPITYAYGWARCSPDCRPVAATQRYRVTSADVGSQLAAVVSARNAI
                     GTVNATLRTGAVAAKVASTTKLKRLSPFPLMLIDGRVAGRITSISTLRLRRVPGGATI
                     GIRCAGRGCPFKRSTIKVRKGKSRTVALRRLQRRMRAGTTVVITVRKGETLGKYIRLR
                     FRRGTAPARVDRCVAPKSSKPVKCS"
     gene            1073..1822
                     /locus_tag="VFY44_05410"
     CDS             1073..1822
                     /locus_tag="VFY44_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909910.1"
                     /translation="MLLSRRSIALAAAAAVSVAAGAQAQTPPAGQPVNRSIPVVTQSG
                     STLSTTTGSWIGDTRPFTYEWLRCADASTTTCFVVPGQTAATYAITGLDAGARLRSRV
                     TASNQLGSRTALSEPTPPVAVSSPVTTGPRALPRLSPFPVLVVTGRTRGARTRITGLL
                     VRGPRGARVAVSCRGACRAKPFGGRIPAGRRARLKKGELLYRAGAILEIRVTGRAKVG
                     KFTRLRMRRGRAPARLDRCLAPGATAPVACS"
     gene            1931..3607
                     /locus_tag="VFY44_05415"
     CDS             1931..3607
                     /locus_tag="VFY44_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909911.1"
                     /translation="MALTALRAHLSDPKRMFSFVGVIGASIALSDCDGCDYPIKEESG
                     AEIVVDGTLEAGKPAAVAVYGQVGKGEKCPTSLRISYNEPSTPGLTPEQTWNESETVT
                     AQRVDEDAPQSAGKPCRLVAKGRALTVLARGTKPRKVEITAAIHVKEKDEIRRSTATK
                     TVTVNPPGGRPVQQPVSANPPAGPVARIVVDRAPIVREASNVFLDGRASYDPDGSIAK
                     YEWDLDNNGAYDHTGPEVQTTPDPGSSQLFRLRVTDSSGLQTVTTRTVPVVGAAQTVF
                     ADPNFGPHDVRVGNSYAVDPAEFINDADKAELDWETDGVLEQTLTGDLSTQTFTSRAY
                     AAPGPQIQSVRYSVTADPALAPATYYRVASVAPAARAGLAAKAKKLTLSLNSGTVKIK
                     RRGRLVVGNNGFRITGMRVTGKGRGGVPRGTPRSLRGAFKAIASGRYAAALDGTANLL
                     GRQVINSGKATVVVRSSKSRRTQVCLTVTQPAGGGPGRVAILGATGKAKGLTGKGVTP
                     PAIVTASGRPVFDRAVLTARKGKARGLSRACKALARDLDGKKAKKKRRKG"
     gene            complement(3639..>4292)
                     /locus_tag="VFY44_05420"
     CDS             complement(3639..>4292)
                     /locus_tag="VFY44_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5909912.1"
                     /translation="ALAQAERARELGGALGWEVNVFSWRHPAVRALLGLGEMERAGEV
                     AAEAVAHARRWGARSYVAEALRTQARAEPARELELLEEAVTLLEGGERRLERAHASVE
                     LGLALGRAGQAPGAREVLRRGLDLADACTATALAVRARDGLVATGARPRRARSSGRAA
                     LTPAELRVARLAADGLTNRQIAEALFVTVKAVKWHLGNIYRKLEIDSRDRLGEALGR"
BASE COUNT          594 a         1678 c         1520 g          500 t
ORIGIN      
        1 ctgtggtccc cagaaccctc ctcatcgcgc tggtcatgct ggtcgccctg cccggggtgg
       61 cacaggcggc cgtgtcgacc ggcgacgagc cggagccggc gaacgggctc acgccgccca
      121 ccgccaccca ggacccgtcg atctccgggt cggcgaccgt gggtcagagc ctcacggcga
      181 acctcggcac ctgggactca cccccgctga cgtccgtcga cctcgaccac atctggttgc
      241 ggtgcagcgc gtccggcacc gggtgcgccc aggtggccgg cgcgacgccc tcatacgcac
      301 tgacaggcgc tgacgtcggg cggatcatca agctcagggt gcgcgggacg acccagggcc
      361 tgggcagcgg ttaccgcgag cgcgacgcca ccggcgtcgt catctcggcc gcccccgtct
      421 tccccgtcgc ccccagcaac gtgaccccgc ccacgatcac cggcaccgcc cgcgagggcg
      481 aggtgctgac gctgaacacg ggcacgtgga cgggcagcga cccgatcacc tacgcctacg
      541 gctgggcccg ctgctccccg gactgccgcc cggtggcggc cacgcagcgc taccgcgtga
      601 ccagcgccga cgtgggcagc cagctggcgg cggtcgtgag cgcgcgcaac gccatcggca
      661 cggtcaacgc gacgctgcgg accggcgccg tggccgcgaa ggtggccagc accaccaagc
      721 tcaagcggct ctcccccttc cccctgatgc tcatcgacgg gcgcgtggcc ggccgcatca
      781 ccagcatctc cacgctgcgc ctgcgccgcg tgccgggcgg cgcgaccatc ggcatccgct
      841 gcgccgggcg cggctgcccg ttcaagcgga gcacgatcaa ggtgcgcaag ggcaagagcc
      901 ggaccgtcgc cctgcgccgc ctgcagcgcc ggatgcgcgc gggcacgacc gtcgtgatca
      961 ccgtgcgcaa gggcgagacg ctgggcaagt acatccggct gcgcttccgc cgtggcaccg
     1021 cccccgcgcg ggtggaccgg tgcgtggcgc cgaagtcctc caagcccgtc aagtgctcct
     1081 gagccgccgc agcatcgccc tggcggcggc cgccgcggtg tccgtcgccg cgggggcgca
     1141 ggcgcagacg ccgcccgccg gccagcccgt gaaccggtcc attccggtgg tcacccagtc
     1201 gggctcgacc ctgagcacca cgaccgggag ctggatcggc gacacgcggc cgttcacgta
     1261 cgagtggctg cgctgcgccg acgcgtcgac caccacctgc ttcgtcgtcc cggggcagac
     1321 ggcggccacc tacgcgatca ctggcctgga cgcgggcgcg cggctgcggt cgcgggtcac
     1381 cgcctcgaac cagctgggct cgcgcacggc gctgtccgag cccacgccgc cggtggccgt
     1441 ctcctcgccc gtgaccaccg gcccgcgggc gctgccgcgc ctgtccccct tccccgtgct
     1501 ggtcgtcacc ggccgaacgc ggggcgcgcg gacccgcatc accgggctcc tggtgcgcgg
     1561 gccgaggggc gcccgcgtcg cggtcagctg ccgcggggcg tgccgggcca agccgttcgg
     1621 cggccgcatc cccgccggcc gccgggcccg cctgaagaag ggtgagctgc tctaccgggc
     1681 cggcgcgatc ctggagatcc gcgtgaccgg ccgcgccaag gtgggcaagt tcacccgcct
     1741 gcgcatgcgg cgcggccgtg ccccggcgcg gctggaccgc tgcctggcgc ccggcgccac
     1801 ggcgcccgtg gcctgctcct aggccaaaac cctggacccc cggtccaggg cgcccggggc
     1861 cccttggtcc cagagtggcg gcacccacgt ccggcacggg agccgggcgg ccagctcgcg
     1921 aggaatccac ttggctctca ccgctctgcg cgcccacctg tcggacccga agcgcatgtt
     1981 ctccttcgtc ggcgtgatcg gcgcgtccat cgccctctcc gactgcgacg gctgcgacta
     2041 cccgatcaag gaagagtcgg gcgccgagat cgtggtggac ggcacgctcg aggccggcaa
     2101 gccggccgcc gtggcggtct acggccaggt cggcaagggc gagaagtgcc ccaccagcct
     2161 gcggatctcc tacaacgagc cgagcacccc gggcctgacg cccgagcaga cctggaacga
     2221 aagcgagacg gtcaccgcgc agcgcgtcga cgaggacgcc ccccagagcg cgggcaagcc
     2281 ctgccgcctg gtggcgaagg gccgcgctct gaccgtgctg gctcgcggca ccaagccccg
     2341 caaggtggag atcaccgccg cgatacacgt gaaggaaaag gacgagatcc gccgctccac
     2401 ggccacgaag acggtcacgg tgaacccacc cggaggcagg ccggtccagc agcccgtctc
     2461 ggccaacccg cccgccggcc cggtggcccg gatcgtggtg gaccgcgcgc ccatcgtccg
     2521 tgaggcctcg aacgtgttcc tggacggccg cgcctcctac gaccccgacg gctcgatcgc
     2581 caagtacgag tgggacctgg acaacaacgg cgcctacgac cacaccggcc ccgaggtcca
     2641 gacgaccccc gatcccggtt cgtcccagct gttccgcctg cgtgtgaccg acagctcggg
     2701 cctccagacc gtgaccaccc gcaccgtgcc cgtcgtgggt gcggcccaga ccgtgttcgc
     2761 cgacccgaac ttcgggccgc acgacgtgcg cgtgggcaac tcctacgccg tcgacccggc
     2821 cgagttcatc aacgacgccg acaaggcgga gctggactgg gagaccgacg gcgtgctcga
     2881 gcagacgctg accggcgatc tgagcacgca gacgttcacc agccgggcgt acgccgcccc
     2941 cgggccgcag atccagtcgg tgcggtactc ggtgaccgcc gacccggcgc tggctccggc
     3001 cacctactac cgggtggcca gcgtggcccc tgcggcgcgg gcagggctcg ccgcgaaggc
     3061 caagaagctg acgctctcgc tgaacagcgg caccgtgaag atcaagcggc gaggccgcct
     3121 ggtcgtgggg aacaacgggt tccggatcac gggcatgcgt gtgaccggca agggccgcgg
     3181 cggcgtgccc aggggaacgc ccaggtcgct tcgcggcgcc ttcaaggcga tcgcctcggg
     3241 tcgctacgcg gccgcgctgg acggcacggc caacctcctg ggccgccagg tgatcaacag
     3301 cggcaaggcg accgtggtgg tgcgtagctc caagagccgc cggacccagg tctgcctgac
     3361 cgtgacccag ccggccggtg gcggtccggg ccgggtggcg atcctcggcg ccacgggcaa
     3421 ggccaagggc ctgaccggga agggcgtgac gccgccggcg atcgtcaccg cctcgggccg
     3481 gcccgtgttc gacagagcgg tcctgaccgc ccgcaagggc aaggccaggg gcctcagccg
     3541 cgcctgcaag gccctggccc gcgacctgga cggcaagaag gccaagaaga agcgccgcaa
     3601 gggctagggg tacggccgcg cgggaggcgc tcgggcggtc agcgcccgag cgcctccccg
     3661 agccggtcgc gcgagtcgat ctcgagcttg cggtagatgt tgcccaggtg ccacttgacc
     3721 gccttgacgg tcacgaacag ggcctcggcg atctgacggt tggtgagccc gtcggccgcc
     3781 aggcgggcca cgcgcagctc ggcgggggtc agcgcggcgc gtcctgagga ccgcgcccgg
     3841 cgggggcggg cccccgtggc caccagccca tcgcgcgcgc ggactgccag tgccgtggcc
     3901 gtgcacgcgt ccgccaggtc gagaccgcgt cgcaggacct cgcgggcgcc gggagcctgg
     3961 cccgcccggc ccagcgccag ccccagctcc acgctcgcgt gggcgcgctc cagccgccgc
     4021 tcgcccccct ccagcagcgt cacggcctct tccagcagct ccagctcgcg tgcgggctcg
     4081 gcccgggcct gggtccgcag cgcctcggcc acgtagctcc gcgcgcccca gcggcgcgcg
     4141 tgggccaccg cctcggcggc gacctcgccg gcccgctcca tctcgcccag gcccaggagc
     4201 gcgcgcacgg cgggatggcg ccagctgaac acgttgacct cccagcccag ggctcccccg
     4261 agctcgcgcg cccgttcggc ctgcgccagc gc
//
LOCUS       DASUPW010000237         3427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1994537, whole genome shotgun sequence.
ACCESSION   DASUPW010000237 DASUPW010000000
VERSION     DASUPW010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1994537"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VFY44_05425"
     CDS             <1..461
                     /locus_tag="VFY44_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5909913.1"
                     /translation="MLALTEHPDQRAHLLGDIDGRMDTAVEEFVRWASPVMTFRRTAT
                     QDTRVRDVEIAAGDKVVLFYPSGNRDERAIDDPYAFDVSRDPNRHLGFGGGGPHYCMG
                     AALAKLQLRTLFGQILHRYPELEVADPTYLVGNFVNGVSALPMQRGPAAA"
     gene            complement(458..1918)
                     /locus_tag="VFY44_05430"
     CDS             complement(458..1918)
                     /locus_tag="VFY44_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEX5909914.1"
                     /translation="MSTPGSTQLLIGGDWRDAAGGASFERTDPFTGDPAGSAAAGGRA
                     DARAAADAAAAAFGEWSATPPAGRRAGLQKAAALLRERAQDIAAIVTAETGGTFGWGM
                     FNCDLAAGMLEEAAAQTTAVTGEVIPSNVPGLTAMSVRRPAGVVVGIAPWNAPVILGT
                     RAVATPLAFGNTVVLKASEVCPRTHAAIAEALHDAGLPAGAVNLVTHEADAAAEVVDE
                     LIAHPAVRRVNFTGSTRVGRLIAENAARHLKRVLLELGGKAPLVVLGDADLDEAVAAA
                     KFGAFMHQGQICMSTERVVADVTVADEFASALASKAGELTVGDPRDPATQIGPLVGPA
                     AIERVSGLVEDARSKGAQVLCGGQADGPLFPPTVVHGVTAEMDIYTEESFGPVVAVTA
                     VDGADEAVAVANDTEYGLAAAVFGSDVPTALDVARRIESGICHVNGATVHDEPQMPFG
                     GVKASGWGRFGGTAAIEEFTELRWLTVQDGPRHYPI"
     gene            complement(1958..>3427)
                     /locus_tag="VFY44_05435"
     CDS             complement(1958..>3427)
                     /locus_tag="VFY44_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEX5909915.1"
                     /translation="TGITQQMVDTAPPPGDVLEEFAALLEGRVLVAHNASFDARVLEQ
                     AFQRAGLEWPDPPVLCTVALARKCAPLVRQRKLALLADALGIQVDVVHRALPDATTCA
                     QILCALFPRLCAAAPTVADAVAALTSSRRRGRREPAKQAARRAPEDRPDMKHLPKDPG
                     VYVFRDERGRPLYVGKSVSLRTRARAHFCAPSGWTERAEVADYRPTNSELGALVLENR
                     LIKQWRPPGNKALKRTDKWAYVLCRLDVEYPVLEVSAEPASGRAVSIGPVRGKKLAAE
                     LADHLTSMYRLRHCGRRMTRREHPSIYGQMGRCCSPCLGDLDPNAYRGQVDKVLALFE
                     HEHGAAEPLLAEIDDRMAAAARERRYERAAALLRRRERLEALLGRLDGVLRAVHADPR
                     LVLARHPVQARFDALWLVNGRVADWGPLPEEPGEVARRSRSVLDAAGRAERTSVPAED
                     VDEVRIASAWMAEHRPPELSLAEAVEPERVERFVAAAAA"
BASE COUNT          423 a         1335 c         1252 g          417 t
ORIGIN      
        1 ccatgctggc cctgaccgag cacccggacc agcgcgcgca cctgctgggg gacatcgacg
       61 gccggatgga caccgcggtc gaggagttcg tgcgctgggc cagcccggtc atgaccttcc
      121 gccgcaccgc cacgcaggac acgcgcgtgc gcgacgtcga gatcgccgcg ggggacaagg
      181 tcgtgctgtt ctacccgtcg ggcaaccggg acgagcgcgc gatcgacgac ccgtacgcgt
      241 tcgacgtcag ccgcgacccc aaccgccacc tgggcttcgg cggcggcggt cctcactact
      301 gcatgggcgc ggcgctggcc aagctgcagc tgcgcacgct cttcggccag atcctgcacc
      361 gctaccccga gctcgaggtg gccgacccca cctatctggt cggcaacttc gtgaacggcg
      421 tcagcgcgct gccgatgcag cgcgggccgg cagccgccta gatggggtag tggcgcgggc
      481 cgtcctggac cgtcagccag cgcagctcgg tgaactcctc gatggccgcg gtgcccccga
      541 aacggcccca gccgctggcc ttgacgccgc cgaagggcat ctgcggctcg tcgtgcacgg
      601 tcgccccgtt cacgtggcag atgcccgact cgatccgccg cgccacgtcc agcgccgtgg
      661 gcacgtcgga gccgaacacc gcggcggcca ggccgtactc ggtgtcgttg gccacggcga
      721 ccgcctcgtc ggcgccgtcg accgcggtca ccgccacgac cgggccgaac gactcctccg
      781 tgtagatgtc catctcggcc gtaacgccgt gcacgacggt gggcgggaac agcggcccgt
      841 cggcctgacc gccgcacagc acctgcgcgc ccttcgagcg cgcgtcctcg accaggcccg
      901 acacgcgctc gatcgcggcg gggcccacca gcgggccgat ctgggtggcc gggtcgcgcg
      961 ggtcgcccac ggtcagctcg cccgccttgg acgccagcgc cgaggcgaac tcgtcggcca
     1021 ccgtgacgtc ggccacgacg cgctcggtgg acatgcagat ctggccctgg tgcatgaacg
     1081 cgccgaactt ggcggcggcc acggcctcgt ccaggtcggc gtcgcccagc accaccagcg
     1141 gcgccttgcc gcccagctcc agcagcacgc gcttgaggtg ccgggccgcg ttctccgcga
     1201 tcaggcggcc cacgcgcgtg gagccggtga agttcacgcg gcgcaccgcc gggtgggcga
     1261 tcagctcgtc gaccacctcg gcggccgcgt cggcctcgtg ggtcaccagg ttcaccgcgc
     1321 ccgcgggcaa gccggcgtcg tggagcgcct ccgcgatcgc cgcgtgcgtg cgcgggcaca
     1381 cctcggacgc cttcaggacc acggtgttgc cgaaggccag cggggtggcc accgcgcgcg
     1441 tgcccaggat caccggggcg ttccagggcg cgatgcccac caccaccccc gcgggccggc
     1501 gcacggacat cgccgtcagg ccgggcacgt tggacgggat cacctcgccg gtcacggccg
     1561 tggtctgggc ggccgcctcc tcgagcatcc cggcggccag gtcgcagttg aacatccccc
     1621 agccgaacgt gccgccggtc tcggcggtca cgatcgcggc gatgtcctgc gcgcgctcgc
     1681 gcagcagtgc cgccgccttc tggagaccgg cacgccggcc ggccggggga gtggccgacc
     1741 actcgccgaa ggcggccgcc gccgcgtccg cggccgccct ggcgtcggcc cgcccgcccg
     1801 cggcggccga cccggcgggg tcgccggtga acgggtcggt gcgctcgaag ctggcgccgc
     1861 cggcggcgtc gcgccagtcg ccgccgatca ggagttgggt gctgccgggc gtgctcatgc
     1921 ccgaggggta cccgccgggc gcgggaggta gaccgcccta ggcggccgcc gcggccacga
     1981 accgctcgac gcgctccggc tccaccgcct cggccagcga cagctcgggc gggcggtgct
     2041 cggccatcca ggccgaggcg atccgcacct cgtccacgtc ctcggccggg accgacgtgc
     2101 gctcggcgcg gcccgccgcg tccagcaccg agcggctgcg gcgcgcgacc tcgccgggct
     2161 cctcgggcag cgggccccag tcggccaccc ggccgttgac cagccacagc gcgtcgaagc
     2221 gggcctggac cgggtggcgg gccagcacca gccgcgggtc ggcgtgcacg gcgcgcagca
     2281 cgccgtccag gcggcccagc agcgcctcca ggcgctcgcg ccggcgcagc agggcggcgg
     2341 cccgctcgta gcggcgctcg cgcgcggcgg cggccatccg gtcgtcgatc tcggccagca
     2401 gcggctcggc cgcgccgtgc tcgtgctcga acagggccag caccttgtcc acctggccgc
     2461 ggtaggcgtt ggggtccagg tcgccgaggc agggcgagca gcagcggccc atctgcccgt
     2521 agatcgacgg atgctcgcgc cgggtcatcc gccggccgca atggcgcagc ctgtacatcg
     2581 aggtcaggtg gtcggccagc tcggcggcca gcttcttgcc ccgcaccggg ccgatgctca
     2641 ccgcccgccc cgacgccggc tcggccgaga cctccaggac cgggtactcg acgtcgaggc
     2701 ggcacaggac gtaggcccac ttgtcggtgc gcttgagcgc cttgttcccc ggcggccgcc
     2761 actgcttgat caggcggttc tccagcacca gcgcgcccag ctcggagttc gtgggccggt
     2821 agtcggccac ctccgcgcgc tcggtccagc cggacggcgc gcagaagtgc gcccgggcgc
     2881 gcgtgcgcag cgagaccgac ttgcccacgt acagcggccg gccgcgctcg tcgcggaaca
     2941 cgtagacgcc ggggtccttg ggcaggtgct tcatgtccgg ccggtcctcg ggtgcccgcc
     3001 gcgccgcctg cttcgccggc tccctgcgcc cgcgccgccg cgacgaggtc agcgcggcca
     3061 cggcgtcggc cacggtcggc gccgccgcgc acagccgcgg gaacagcgcg cacaggatct
     3121 gggcgcaggt ggtggcgtcg ggcagcgccc ggtggaccac gtcgacctgg atccccagcg
     3181 cgtccgccag cagggcgagc ttgcgctggc gcaccagcgg cgcgcacttg cgcgccaggg
     3241 cgacggtgca cagcacgggc ggatcgggcc actccagccc ggcgcgctgg aaggcctgct
     3301 ccaggacccg cgcgtcgaag gaggcgttgt gggccaccag cacccgtccc tccagcagcg
     3361 ccgcgaactc ctccagcacg tcgccgggcg ggggcgcggt gtccaccatc tgctgggtga
     3421 tgccggt
//
LOCUS       DASUPW010000238         2972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1717049, whole genome shotgun sequence.
ACCESSION   DASUPW010000238 DASUPW010000000
VERSION     DASUPW010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1717049"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1362
                     /locus_tag="VFY44_05440"
     CDS             <1..1362
                     /locus_tag="VFY44_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEX5909916.1"
                     /translation="LAPDRGAFVGGPGGTAVASLSPELFLERRGRRVRTAPIKGTRPR
                     SADAAELEGSAKDRAENTMIVDLMRNDLGRVCEPGSVTVDALARARPHAGVWHLVSEV
                     SGDLRADAGDADLLRACVPPGSVTGAPKIAAQNVIAEVESTGREAYTGAIGFASPAAG
                     LELSVAIRTFEFSGPRAWLGVGGGVVADSDPAAEARECAVKAAPLLAAIGARSTFDPP
                     GDPNMPRVARRGPTPTPRPDPEAGVFSTLLVRDGLAIAAAGHLARLGASVAELYGAEL
                     PADLADRIETAAAAHPGPGRLRVTYAPGDAEPATIVVKPFDPDRPPATLAPVTVPGGI
                     GAHKWADRRLLDALAEEAAPALPLLVDLDGYVLESWGANVFARLPGGTLATPPLDGRI
                     LPGVARAQLLKVHTGAAERRLTLDEVLAAEEVFLTSALGVQVAEIAPEPTSASTSTPS
                     PTR"
     gene            complement(1296..2132)
                     /locus_tag="VFY44_05445"
     CDS             complement(1296..2132)
                     /locus_tag="VFY44_05445"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001392.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX5909917.1"
                     /translation="MDLWQAIILGLVEGFTEFLPISSTGHLTILEKLMGFQIDDPDVT
                     AFTAIIQSGAVLATLLYLRGDIGRILTALAQGVADARERETRDFRFGIAVAVGCIPIA
                     IVGLAFKDQIETTLRSLWFVGGALILWSGVMYYADVNATQERGEDDVRTKDTLIIGIV
                     QCLALIPGVSRSGATMSAGLLRGLDRVTVTRLSFFLSIPALGAATVLQAATEYDRIST
                     GVGWGATLLATAVSFVAAYVAISWLLRYIASHNYSVFIIYRVGLGVLVLALVGSGAIS
                     AT"
     gene            complement(2157..>2972)
                     /gene="fabI"
                     /locus_tag="VFY44_05450"
     CDS             complement(2157..>2972)
                     /gene="fabI"
                     /locus_tag="VFY44_05450"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF005908.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEX5909918.1"
                     /translation="VAPPGLRTGAAVMLEGRKLLITGVVTRQSIAFAVAEQAQRDGAE
                     VLLTGFGRARRLTERAAKRLPDPPEVLELDVNKPEDVEALRDELERRWGQVDGVLHAI
                     AYAPEEALGGNFLETSTESAQQAFATSAYSLKALGAGLRPLLAAAPDGGSVVGLDFDA
                     SVAWPAYDWMGVSKAALESVSRYLARDLGCDGTRVNLVAAGPIATAAAGGIPGFDDLA
                     GAWRRQAPLGWDSDDAGAVADAVLFLLSDRSRAISGEILHADGGFHAMGAVPA"
BASE COUNT          392 a         1127 c         1101 g          352 t
ORIGIN      
        1 ctggcccccg accgcggcgc cttcgtgggc ggtcccggcg gcacggccgt ggccagcctg
       61 tcgcccgagc tgttcctgga gcgccgcggg cggcgggtgc gcacggcgcc gatcaagggc
      121 acgcggccgc gctcggctga cgccgccgag ctggagggct cggccaagga ccgggcggag
      181 aacacgatga tcgtggacct gatgcgcaac gacctgggcc gcgtgtgcga gcccgggtcg
      241 gtgacggtgg acgcgctggc ccgggcgcgg ccgcacgcgg gcgtgtggca cctggtctcc
      301 gaggtctcgg gcgacctgcg cgcggacgcc ggcgacgccg acctgctgcg cgcctgcgtc
      361 ccaccgggct cggtgaccgg cgcgccgaag atcgcggcgc agaacgtgat cgccgaggtg
      421 gagtcgaccg gccgcgaggc gtacacgggc gccatcggct tcgcctcccc cgccgccggc
      481 ctcgagctca gcgtggccat ccgcaccttc gagttctcgg gcccccgcgc ctggctgggc
      541 gtgggcggcg gcgtggtggc cgactccgac ccggccgccg aggcgcgcga gtgcgcggtc
      601 aaggccgccc ccctgctggc ggccatcggc gcgcgcagca cgttcgatcc gcctggcgac
      661 cccaacatgc cgcgcgtggc ccggaggggg cccacgccga ccccgcggcc cgaccccgag
      721 gccggcgtgt tcagcacgct gctggtgcgc gacggcctgg ccatcgccgc cgccggccac
      781 ctggcccgcc tgggcgccag cgtggccgag ctctacggcg ccgagctgcc cgccgacctg
      841 gccgaccgga tcgagaccgc cgccgccgcg cacccgggcc ccggccgcct gcgcgtcacc
      901 tacgcgcccg gagacgccga gcccgccacg atcgtcgtca agcccttcga tccggaccgc
      961 ccacccgcga cgctggcgcc ggtcaccgtc cccggcggga tcggcgcgca caagtgggcc
     1021 gaccgccgcc tgctggacgc gctggccgag gaggcggcgc ccgcgctgcc gctgctggtg
     1081 gacctggacg ggtacgtgct cgagagctgg ggcgcgaacg tgttcgcgcg cctgcccggc
     1141 gggacgctgg ccacgccccc gctggacggc cggatcctgc ccggcgtggc gcgcgcgcag
     1201 ctgctgaagg tgcacaccgg cgccgccgag cgccggctca cgctggacga ggtcctggcc
     1261 gccgaggagg tcttcctgac cagcgccctg ggcgttcagg tggcggagat cgcgcccgag
     1321 ccgaccagcg ccagcaccag cacgcccagc cccacccggt agatgatgaa caccgagtag
     1381 ttgtggctgg cgatgtagcg cagcagccac gagatggcca cgtaggcggc cacgaacgag
     1441 acggcggtgg ccagcagcgt cgccccccac cccacgccgg tggagatgcg gtcgtactcg
     1501 gtggccgcct ggagcacggt ggcggcgccc agcgccggga tcgacaggaa gaacgagagc
     1561 cgcgtcacgg tcacgcggtc gagcccgcgc agcaggccgg ccgacatggt ggcgccggag
     1621 cgcgacacgc ccgggatcag cgccaggcac tggacgatgc cgatgatcag cgtgtccttg
     1681 gtgcgcacgt cgtcctcgcc gcgctcctgg gtggcgttca cgtcggcgta gtacatgacg
     1741 ccgctccaca ggatcagcgc gccgcccacg aaccacaggc tgcgcagggt cgtctcgatc
     1801 tggtccttga aggccagccc gacgatggcg atggggatgc agcccaccgc caccgcgatg
     1861 ccgaaacgga agtcgcgcgt ctcgcgctcg cgcgcgtcgg caaccccctg cgccagcgcg
     1921 gtcaggatgc gcccgatgtc gccgcgcagg tagagcagcg tggccaggac ggcgccgctc
     1981 tggatgatcg cggtgaacgc ggtcacgtcg gggtcgtcga tctggaagcc catcagcttc
     2041 tcgaggatcg tcagatggcc cgtcgaggag atcgggagga actcggtgaa tccctcgacc
     2101 agccccagga tgattgcctg ccagaggtcc aagcggcgca ggctagctgc gcggggctag
     2161 gccgggacgg cccccatggc gtggaacccc ccgtcggcgt gcaggatctc gccgctgatc
     2221 gcgcgtgagc ggtcgctcag gaggaagagc acggcgtcgg ccaccgcgcc cgcgtcgtcg
     2281 gagtcccagc ccagcggggc ctggcgccgc caggcgccgg ccaggtcgtc gaagccgggg
     2341 atgccgcccg ccgccgcggt ggcgatcggg ccggcggcca ccaggttcac gcgcgtgccg
     2401 tcgcagccca ggtcgcgggc caggtagcgc gagaccgact ccagcgccgc cttcgacacg
     2461 cccatccagt cgtaggcggg ccaggccacg gaggcgtcga agtccagtcc gaccacgctg
     2521 ccgccgtcgg gcgcggcggc cagcagcggg cgcaggcccg cgcccagcgc cttgagcgag
     2581 tacgcgctgg tggcgaacgc ctgctgcgcg ctctcggtcg aggtctccag gaagttgccg
     2641 cccagggcct cctcgggcgc gtaggcgatg gcgtgcagca ccccgtccac ctgcccccag
     2701 cgccgctcga gctcgtcgcg cagcgcctcc acgtcctcgg gcttgttcac gtccagctcc
     2761 agcacctcgg gcggatcggg caggcgcttg gccgcccgct cggtcagccg ccgagcgcgc
     2821 ccgaagccgg tcagcagcac ctcggcgccg tcgcgctggg cctgctcggc cacggcgaac
     2881 gcgatcgact gcctggtcac gactccggtg atcaggagct tcctgccctc cagcatcaca
     2941 gcggcaccgg tacgaaggcc gggcggcgcg ac
//
LOCUS       DASUPW010000239         2584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1301001, whole genome shotgun sequence.
ACCESSION   DASUPW010000239 DASUPW010000000
VERSION     DASUPW010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301001"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="VFY44_05455"
     CDS             complement(<1..1088)
                     /locus_tag="VFY44_05455"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5909919.1"
                     /translation="MCAHRLHPEHDITVFEAEDRVGGHTNTVRVDAADGTHWVDTGFI
                     VFNDRNYPNFEPLLAELGVATQKSRMSFSVSDGSGGFEYSGSPRGLFAQPGNAVDPRF
                     LRMVADLVRFNREVRGLLELAPDEGPSLREFLDEGGYGEWFTERLIVAQASAVWSADP
                     EQMWSFPAAFLARFFHNHGMLGFTGRPQWRTVTGGSRRYLEEITRPFADRIRTGAPVR
                     TIERTAEGVLVSADGCETELFDEVVVAAHADQALGMLARPTAAERDVLGAVAYQPNQA
                     VLHTDTSVMPRRRAAWASWNAHLVPGSREARLTYWMNLLQSLERTPEGVLVRAAGHET
                     ELFDEVVVAAHADQALGMLDRPTEAERDV"
     gene            1243..1629
                     /locus_tag="VFY44_05460"
     CDS             1243..1629
                     /locus_tag="VFY44_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5909920.1"
                     /translation="MDTSRLKALPLFEGVSDEDLDKIAPFVSEVSVSEGKHLVDEGDY
                     AYEFMAIEEGEAEVVRGDETLATLSAGDYFGEIGLLEDDRRNATVIARSPMRLLTLDQ
                     WDMKRLEKAIPSAAEKIRETVRSRRQ"
     gene            1677..>2584
                     /locus_tag="VFY44_05465"
     CDS             1677..>2584
                     /locus_tag="VFY44_05465"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX5909921.1"
                     /translation="MVGLIAGSGQDDPAGTTDTAEPPASRGGGRAPAEAARLPLERRV
                     GELLVMSFDGPTAPGYIVRRLRSGEGTGVILFAKNAPDAASLRSLTGGLQRAAGGAAL
                     VMTDQEGGDIRTLPFSPPEASQGRQGSVDVVTASARDAARGLRAGGVNVNLAPVGDVA
                     DSLGSVVAGRAFPGGGREVAELTAASIRAHGRERVGATAKHFPGLGRATENTDDTSVT
                     VPASRAELEGTDLLPFTAAVRAGTPLVMSSHALYPALDRSRIASQSPAILKGLLRGRL
                     DFDGAVVTDSIEAQAVLDRSAVGVAA"
BASE COUNT          350 a          922 c          970 g          342 t
ORIGIN      
        1 acgtcgcgct ccgcctccgt ggggcggtcc agcatgccca gcgcctggtc ggcgtgggcg
       61 gccaccacca cctcgtcgaa cagctcggtc tcgtgccccg cggcgcggac caggaccccc
      121 tcgggcgtgc gctccaggga ctggagcagg ttcatccagt acgtcagcct cgcctcgcgc
      181 gagcccggga ccaggtgggc gttccaggag gcccaggcgg cacggcggcg gggcatcacc
      241 gacgtgtcgg tgtgcagcac ggcctggttg ggctggtagg ccaccgcgcc cagcacgtcg
      301 cgctcggcgg ccgtgggccg ggccagcatg cccagcgcct ggtcggcgtg ggccgccacc
      361 acgacctcgt cgaacaactc ggtctcgcag ccgtcggcgc tgaccaggac gccctcggcg
      421 gtgcgctcga tcgtccgcac cggggcgccg gtgcggatcc tgtcggcgaa cgggcgggtg
      481 atctcctcca ggtagcggcg cgagccgccg gtcaccgtgc gccactgcgg ccggccggtg
      541 aagcccagca tgccgtggtt gtggaagaag cgggccagga aggcggcggg gaacgaccac
      601 atctgctccg ggtcggccga ccacacggcg gaggcctggg ccacgatcag ccgctcggtg
      661 aaccactcgc cgtagccacc ctcgtccagg aactcgcgca gcgagggccc ctcgtcaggc
      721 gccagctcca gcaggccgcg cacctcgcgg ttgaaccgca ccaggtcggc gaccatgcgc
      781 aggaagcgcg ggtccaccgc gttgccgggc tgggcgaaca gcccgcgtgg gctgccgctg
      841 tactcgaagc cgccgctgcc gtcgctgacc gagaagctca tccgcgactt ctgcgtggcc
      901 acccccagct cggccagcag cggctcgaag ttggggtagt tgcggtcgtt gaagacgatg
      961 aagccggtgt cgacccagtg ggtgccgtcg gcggcgtcga cacgcacggt gttggtgtgc
     1021 ccgcccacgc ggtcctcggc ctcgaacacg gtgatgtcgt gctcggggtg gagccgatgg
     1081 gcgcacacca atcccgagat accggtgccg acgactgcta tccgcgaggc catggttcat
     1141 tcctacggac gcaacggccc tcccgatcag tcgtgcgtgc gcgccaagtg ggttagattc
     1201 cgcacggagg tcgccggtac atcgcgaatg gaggactgaa ggttggacac cagccgactg
     1261 aaggcgctgc cgttgttcga aggggtatcg gacgaggatc tggacaagat cgcgccgttc
     1321 gtgagcgagg tgtccgtgtc cgagggcaag cacctcgtcg acgaagggga ctacgcgtac
     1381 gagttcatgg ccatcgagga gggcgaggcc gaggtggtcc gcggtgatga gacgctggcc
     1441 acgctgagcg cgggggacta cttcggcgag atcgggctgc tggaggacga ccgccgcaac
     1501 gccacggtca tcgcacgctc gccgatgcgg ctcctgacgc tggaccagtg ggacatgaag
     1561 cggctggaga aggccatccc gtccgcggcc gagaagatcc gggagaccgt ccggtcgcgt
     1621 cggcagtagc gcgccgccgc ctggccgcgc tgggcgcggt cgccctgctc gcgctggtgg
     1681 tgggcctgat cgccggctcg ggtcaggacg acccggccgg gacgacggac acggccgagc
     1741 cgccggcgtc ccgcggtggt ggccgggcgc cggccgaggc cgcccgcctg cccctggagc
     1801 gccgcgtcgg cgagctgctg gtcatgagct tcgacggacc gaccgcgccg ggctacatcg
     1861 tgcgccgcct gcgcagcggc gagggcaccg gcgtgatcct gttcgccaag aacgcgcccg
     1921 atgccgcctc cctgcgctcg ctgaccggcg gcctccagcg cgcggccggg ggcgcggccc
     1981 tggtgatgac cgaccaggag ggcggcgaca tccgcaccct gcccttctcg ccccccgagg
     2041 cctcgcaggg acggcaggga tcggtggacg tcgtgaccgc gtccgcccgc gacgccgccc
     2101 gcgggctgcg cgccggcggc gtgaacgtga acctggcccc ggtgggcgac gtggccgact
     2161 cgctgggctc cgtcgtggcg gggcgcgcgt tccctggcgg ggggcgcgag gtggccgagc
     2221 tgaccgcggc ctccatccgc gcccacgggc gcgagcgggt gggggccacg gccaagcact
     2281 tccccgggct gggccgggcg accgagaaca ccgacgacac ctccgtgacc gtgcccgcct
     2341 cgcgcgcgga gctggagggc acggacctgc tgccgttcac cgccgcggtg cgcgcgggca
     2401 cgccgctggt catgagctcg cacgcgctgt acccggcgct ggaccgcagc cggatcgcct
     2461 cccagtcgcc ggcgatcctg aagggcctcc tgcgcggccg gctggacttc gatggcgccg
     2521 tggtcaccga cagcatcgag gcccaggccg tgctcgaccg ctccgccgtg ggcgtggccg
     2581 ccga
//
LOCUS       DASUPW010000240         4389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1163783, whole genome shotgun sequence.
ACCESSION   DASUPW010000240 DASUPW010000000
VERSION     DASUPW010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163783"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VFY44_05470"
     CDS             <1..788
                     /locus_tag="VFY44_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5909922.1"
                     /translation="MGDGANDAGLSRRHIIDSVEASLRRLGAGHIDLLQVHEWDGQTP
                     LEETLSALDDLVRAGKVRYVGCSNYTGWQLVKAMGVADRRGFEPFVSQQIHYTPQARE
                     AEFELLPAGLDQGLGTLVWSPLAGGLLSGKYRRGQDGPEGSRHVGDWDEPPVHDADAL
                     YDLVDVLAEVGDAHGVSAAQVTLAWLLTRPAVTSLVVGARTEEQLGDNLAAAALELSA
                     EEVERIDAASRPHLPYPLWHQAANASDRLSEADRAYLAPHLEK"
     gene            complement(980..1462)
                     /locus_tag="VFY44_05475"
     CDS             complement(980..1462)
                     /locus_tag="VFY44_05475"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX5909923.1"
                     /translation="MAARPPSDSRSELALWIGLPESNSAGNAHGGVILKLCDEVAGIA
                     AIRHCGRRVVTVGFDRMTFQRPVLVGELVTVRATVNAAWRSSMEVGVRVEKETLRTGA
                     IEHTSTAYITMVALDDDGRPAEVPEIDPQTDDEHRRAREAQVRRDNRLAERTQLDEGR
                     "
     gene            1540..2280
                     /locus_tag="VFY44_05480"
     CDS             1540..2280
                     /locus_tag="VFY44_05480"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEX5909924.1"
                     /translation="MSTVIKAKISRLLDKSEDPGETLDYGYQKQVELLQNVKKGIADV
                     VTSKKRLQMQSTKLEQSVVKLDTQARQAISQGREDLARAALERKTVAQTELQSLDTQI
                     KELETQQEQLTANEKKLRTKIETFRTKKEVIKAQYSAAEAQVKISEAANGVGEEMADL
                     GMAIQRAEDKTEQMKARAGAVEELEAAGTFDDLTQLGGGQDDIDRELAALGAGSQVDD
                     ELSKMKAELGAGGGSGDAPALEEGKEGQ"
     gene            2277..2555
                     /locus_tag="VFY44_05485"
     CDS             2277..2555
                     /locus_tag="VFY44_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909925.1"
                     /translation="MIVRVSGEGQFKLPDEDAERLNDLDNRAVAAVEAGDRAGFAELW
                     AQMLELVRTDGQALEEDELEESDVILPPRDVTFEEAQGEFTGDGLIPD"
     gene            2590..3534
                     /locus_tag="VFY44_05490"
     CDS             2590..3534
                     /locus_tag="VFY44_05490"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEX5909926.1"
                     /translation="MQRHGLAQRAPASAAMDVISRLCGLHAQVASSAEAMLAARVEGP
                     PVVQGMLDRGELVKTWSMRGTLHLLPAHEYPMWQAAFSTFDNFLKPAWSRASGLWPED
                     VERLIAAVGEALEGSLLTREELAAAVEERTDAHTGQKARESWGAVLKPAAWAGKLVYG
                     ASDGRNVRFTAPPPYEPVDPEQALLEVTRRYLALNGPATREDLKRWWGIQSPARAGKM
                     IEALGDEAVQVDVDGEPRWLLAGDVGEAAAAAPSGAEALVPAFDQYVVGLPRDVEALL
                     PAEHKPRVYRQAGWLTPCVVVDGRIAGVWDGDGEERWA"
     gene            3531..4109
                     /locus_tag="VFY44_05495"
     CDS             3531..4109
                     /locus_tag="VFY44_05495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX5909927.1"
                     /translation="MSFHRLCVFAGSSAGADPAYAEGARALAAELLRRDIGMVYGGAQ
                     TGLMGVTADGVLEGGGEVIGVIPTGLGDREIAHPGLSDLQMVSTMHERKARMGDLADG
                     FVVLPGGLGTLEELMEVVTWNQLGIHAKPIGLLDAGGYWAPLEALIGHAADQGFVSYG
                     SAQMLLRDPDPAALLDAMAGWRPPETRRWVDA"
     gene            complement(4106..>4389)
                     /locus_tag="VFY44_05500"
     CDS             complement(4106..>4389)
                     /locus_tag="VFY44_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909928.1"
                     /translation="ADGGEDRKIGMVEEGETYTRLICDGNREVLDYVVGAKGYSENYE
                     ASVKLGTYDLWKLGTDAYIAPGLSGPKTLAADVRAECGCGETVDGGYGS"
BASE COUNT          650 a         1500 c         1671 g          568 t
ORIGIN      
        1 ccatgggcga cggtgccaac gacgccggcc tctcgcgccg gcacatcatc gactcggtgg
       61 aggcgtcgct gcgccgcctg ggcgccggcc acatcgacct gctccaggtg cacgagtggg
      121 acggccagac gccgctggag gagacgctct cggcgctgga cgacctggtc cgcgccggca
      181 aggtgcgcta cgtcggctgc tcgaactaca ccgggtggca gctggtcaag gctatgggcg
      241 tcgccgatcg ccgcggcttc gagccgttcg tcagccagca gatccactac acgccgcagg
      301 cccgcgaggc cgagttcgag ctgctgcccg ccgggctgga ccagggcctg gggacgctgg
      361 tgtggagccc gctcgcgggc gggctgctga gcggcaagta ccgccgcggc caggacggcc
      421 ccgagggctc acgccacgtg ggcgactggg acgagccgcc ggtgcacgac gccgacgccc
      481 tgtacgacct ggtcgacgtg ctggccgagg tcggcgacgc ccacggcgtc tccgcggccc
      541 aggtcacgct ggcctggctc ctgacgcgcc cggccgtgac gtcgctggtg gtgggcgcac
      601 gcacggagga gcagctgggc gacaacctgg cggcggccgc gctggagctg agcgccgagg
      661 aggtcgagcg catcgacgcg gcgagccggc cgcacctgcc gtacccgctg tggcaccagg
      721 cggcgaacgc gtcggaccgc ctgagcgagg ccgaccgggc ctacctggcg ccccacctgg
      781 agaagtagtt ctccgcaggc cgcaccggac ctggctacgg aagtcgccca gatcgacggt
      841 tcatgcgttt gggtggctgt agcgccctaa acgcgcgaac cgttcttcgc ggcgaccggg
      901 acgtgactga acggtcgcag cgccctcgcc ctacccagcc gccgtgtcgc cgatcagctg
      961 gtgcggccaa cggcgagaac tagcgcccct cgtccagctg ggtccgctcg gccaggcggt
     1021 tgtcccgccg tacctgggcc tcgcgggcgc ggcggtgctc gtcgtcggtc tgggggtcga
     1081 tctcgggcac ctcggcggga cggccgtcgt cgtccagggc gaccatcgtg atgtaggcgg
     1141 tggaggtgtg ctcgatcgcg ccggtgcgca gggtctcctt ctccacgcgc acgccgacct
     1201 ccatcgagga gcgccaggcg gcgttcaccg tggcgcgcac cgtgaccagc tcgccgacca
     1261 ggaccgggcg ctggaaggtc atgcgatcga agcccacggt gaccacccgc cggccgcagt
     1321 ggcggatggc ggcgatgccg gcgacctcgt cgcacagctt caggatcacg ccgccgtggg
     1381 cgttgccggc ggagttggac tcggggagcc cgatccacag ggccagctcg gagcgcgaat
     1441 cggacggggg tcgggcggcc aagagccact agtctaggtc gctcaagcgt tcacgaggtt
     1501 ccgaggaggc agcacgacat ggcaggactg ggcggacgga tgtccaccgt catcaaggcg
     1561 aagatctcgc ggctgctgga caagtccgag gatccgggcg agacgctcga ctacggctac
     1621 cagaagcagg tcgagctact gcagaacgtc aagaagggga tcgccgacgt ggtgacctcc
     1681 aagaagcgcc tgcagatgca gtcgaccaag ctcgagcagt ccgtggtcaa gctggacacg
     1741 caggcgcgcc aggcgatctc gcagggccgt gaggatctgg cccgcgccgc gctggagcgc
     1801 aagaccgtgg cccagaccga gctccagtcg ctggacacgc agatcaagga gctggagaca
     1861 cagcaggagc agctgaccgc gaacgagaag aagctgcgca ccaagatcga gaccttccgc
     1921 accaagaagg aagtgatcaa ggcgcagtac tccgccgcgg aggcgcaggt caagatctcc
     1981 gaggccgcca acggcgtcgg cgaggagatg gccgacctgg gcatggccat ccagcgcgcc
     2041 gaggacaaga ccgagcagat gaaggcccgc gcgggcgccg tcgaggagct ggaggccgcc
     2101 ggcacgttcg acgacctgac ccagctgggc ggcggacagg acgacatcga ccgcgagctg
     2161 gccgccctgg gcgccggcag ccaggtggac gacgagctct cgaagatgaa ggccgagctc
     2221 ggcgcgggcg gaggcagcgg cgacgcgcct gccctcgagg aagggaagga gggtcagtga
     2281 tcgtccgtgt gtcgggcgag ggccagttca agctccccga cgaggacgcc gagcgcctga
     2341 acgacctgga caaccgcgcg gtggccgccg tggaggcggg cgaccgcgcc ggcttcgccg
     2401 agctgtgggc gcagatgctg gagctggtcc gcaccgacgg ccaggccctg gaagaggacg
     2461 agctcgagga gtcggacgtg atcctgcccc cgcgcgacgt gaccttcgaa gaggcccagg
     2521 gcgagttcac cggcgacggt ctcatccctg actgagcagc tctcctggga gcaggcggcg
     2581 gcgtggcgca tgcagcgcca cgggctggcg cagcgcgcgc cggcctccgc cgccatggac
     2641 gtgatcagcc ggctctgcgg gctgcacgcg caggtggcgt cgtcggccga ggcgatgctg
     2701 gcggcgcggg tcgagggccc gcccgtcgtc cagggcatgc tcgaccgcgg cgagctggtc
     2761 aagacgtggt cgatgcgcgg cacgctgcac ctgctgccgg cgcacgagta ccccatgtgg
     2821 caggcggcgt tctcgacctt cgacaacttc ctcaagcccg cctggtcgcg cgcctccggg
     2881 ctgtggcccg aggacgtcga gcgcctgatc gcggccgtgg gggaggcgct ggagggctcg
     2941 ctgctcacgc gcgaggagct ggccgccgcg gtggaggagc gcacggacgc ccacaccggc
     3001 cagaaggccc gcgagagctg gggcgccgtg ctcaagcccg cggcgtgggc cggcaagctg
     3061 gtctacgggg ccagcgacgg ccggaacgtc cgcttcaccg caccgccgcc ctacgagccc
     3121 gtggacccgg agcaggcgct gctggaggtc acgcgccgct acctggcgct gaacgggccg
     3181 gccacgcgtg aggacctcaa gcgctggtgg ggcatccagt cgccggcgcg ggcggggaag
     3241 atgatcgagg ctctgggcga cgaggcggtg caggtcgacg tggacggcga gccccgctgg
     3301 ctgctggctg gggacgttgg ggaggcggca gcggcggcgc cctcgggggc ggaggcgctg
     3361 gtcccggcct tcgaccagta cgtggtcggc ctgccgcgcg acgtcgaggc gctgctgccc
     3421 gccgagcaca agccgcgtgt ctaccgccag gccggctggc tgacgccgtg cgtggtcgtg
     3481 gacgggcgga tcgccggggt ctgggacggc gacggcgagg agcgatgggc gtgagcttcc
     3541 accggctgtg cgtgttcgcc ggctccagcg cgggcgccga cccggcctac gccgagggcg
     3601 cccgggccct ggccgccgag ctgctgcggc gcgacatcgg catggtctac ggcggcgcgc
     3661 agaccggtct gatgggcgtg accgccgacg gggtgctgga gggcgggggc gaggtcatcg
     3721 gcgtgatccc caccggcctg ggggaccgcg agatcgcgca ccccgggctg agcgacctcc
     3781 agatggtgtc gaccatgcac gagcgcaagg cccggatggg cgacctggcc gacgggttcg
     3841 tggtgctgcc gggcggcctc gggaccctgg aggagctgat ggaggtcgtc acctggaacc
     3901 agctgggcat ccacgcgaag ccgatcgggc tcctggacgc cggtggctac tgggcgccgc
     3961 tggaggcgct gatcggccac gccgcggacc agggcttcgt ctcctacggc agtgcccaga
     4021 tgctgctgcg cgatcccgac cccgccgcgc tcctggacgc gatggcgggc tggcgcccac
     4081 ccgagacgcg ccgctgggtc gacgcctagc tgccgtaccc gccgtccacg gtctcgccgc
     4141 agccgcactc ggccctgacg tcggcggcca gggtcttggg gccgctgagc ccgggtgcga
     4201 tgtaggcgtc ggttccgagc ttccacaggt cataggtgcc cagcttcacc gacgcctcgt
     4261 agttctcgga gtagcccttg gcgcccacca cgtagtccag gacctcgcgg ttgccgtcgc
     4321 agatcaggcg cgtgtaggtc tcgccctcct ccaccatgcc gatcttgcgg tcctcgccgc
     4381 cgtccgcgc
//
LOCUS       DASUPW010000241         3096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1025291, whole genome shotgun sequence.
ACCESSION   DASUPW010000241 DASUPW010000000
VERSION     DASUPW010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025291"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VFY44_05505"
     CDS             complement(<1..368)
                     /locus_tag="VFY44_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5909929.1"
                     /translation="MSAVDAAAAQGVDEDRVRKAIATIERPAPTGALAATRAFAWRAM
                     LKVRHVPEQLLDVTVTPVMFLLMFMYLYGGAMFGSVDAYRDYIVPGVLVQTILFTTVY
                     SGVVLNTDMTKGVVDRFRSLP"
     gene            complement(365..1342)
                     /locus_tag="VFY44_05510"
     CDS             complement(365..1342)
                     /locus_tag="VFY44_05510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5909930.1"
                     /translation="MTDQLAIEATGLVKHFGDTKAVDGVDLAVRTGAVYGVLGPNGAG
                     KTTTIRMLATLLAPDGGSAKVLGHDVATEGDAVRHKVSLTGQLASVDEDLTGRENLML
                     LGKLLGLRGGAKARADELLEAFGLEQASAKLVKNYSGGMRRRLDIAASIVVTPELMFL
                     DEPTTGLDPRSRNQVWDIVRALVAAGTTILLCTQYLEEADQLAEGIAVIDHGKVIAEG
                     TPAQLKATVGSGALKVRLLDPAERADAERVLASALDGEVDAQGGDASLLTAPCADADR
                     AAAAVSALSTAGIGVADFSLGQPSLDEVFLALTGRPVDEPGPELEEQAA"
     gene            1418..2218
                     /locus_tag="VFY44_05515"
     CDS             1418..2218
                     /locus_tag="VFY44_05515"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX5909931.1"
                     /translation="MDFTFDPGAAAALLVAEALYVRAWRVLGGRGYRVPRGQLAAWHV
                     GLLAMVVALMSPIGGLSDELLVAHMGEHLILADLGAPLMLVGIRSPVYAFLLPAPALK
                     RLARRKRLRAFFRTLRQPLVAVPLWILILYGWHFTFLFENAIRHDWVHLLQHQSFIVG
                     SLLVWWSVIEPKRRRAGGDLWKVPYLIGARLPGMLLGMAFIIMGSVAYADPYAVTAPQ
                     HGWSALTDQQVAGGLMMATDLAVMLFALMFFFYRSAQDHDRAETAAAV"
     gene            2215..2730
                     /locus_tag="VFY44_05520"
     CDS             2215..2730
                     /locus_tag="VFY44_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909932.1"
                     /translation="MRRLAAASALVLALGAGGCSLGGDDEGGGSGGADRGAATATGPE
                     MPPGVVKPGEPPEAAAIRGWSEAVNARQFGRAASFFARDAIVDQGQAIRLPDRSAAAA
                     FSKSLPCKSRVTDIKDEGRTIVAAFDLRPGTGPKSACDGDARVRFTFSGNKFTEFRQL
                     IEPETAPGQSV"
     gene            2730..>3096
                     /locus_tag="VFY44_05525"
     CDS             2730..>3096
                     /locus_tag="VFY44_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5909933.1"
                     /translation="MEVRRLGRSGLRVSTLTLGTMGFAGAGGFADIGDIDVDGARRQL
                     DLCIDAGVNLVDTANMYSDGASEEVLGEALDGGRRDKLMVATKVRFPMGDGANDAGLS
                     RRHIIDSVEASLRRLGAGHI"
BASE COUNT          393 a         1123 c         1135 g          445 t
ORIGIN      
        1 ggcagcgagc ggaaccggtc gaccacgccc ttggtcatgt cggtgttcag caccacgccc
       61 gagtagacgg tggtgaacag gatggtctgc accagcacgc cggggacgat gtagtcgcgg
      121 taggcgtcga ccgagccgaa catcgccccg ccgtagaggt acatgaacat cagcaggaac
      181 atcaccggcg tgaccgtcac gtccagcagc tgctcgggca cgtggcgcac cttgagcatc
      241 gcccgccagg cgaaggcccg ggtggcggcg agcgcgcccg tgggcgcggg ccgctcgatc
      301 gtggcgatcg ccttgcggac ccggtcctcg tccacgccct gtgccgcggc ggcgtcgacg
      361 gcgctcacgc cgcctgctcc tcgagctcgg ggccgggctc gtcgaccggg cggccggtca
      421 gggccaggaa cacctcgtcc aggctgggct ggcccagcga gaagtcggcc accccgatgc
      481 ccgcggtgga cagggcgctg acggcggcgg cggcgcggtc ggcgtccgcg cacggcgcgg
      541 tcagcaggct ggcgtcgccg ccctgggcgt cgacctcacc gtcgagggcg gaggccagca
      601 cgcgctcggc atcggcgcgc tcggcggggt ccagcagtcg caccttcagg gcgcccgagc
      661 cgacggtggc cttgagctgc gcgggggtgc cctcggcgat gaccttgccg tggtcgatca
      721 ccgcgatgcc ctcggccagc tggtcggcct cctcgaggta ctgcgtgcac agcaggatgg
      781 tggtgccggc ggcgaccagc gcgcggacga tgtcccacac ctggttgcgc gagcgcgggt
      841 ccaggccggt cgtgggctcg tccaggaaca tcagctccgg cgtgaccacg atgctggccg
      901 cgatgtccag ccgccggcgc atgccgcccg agtagttctt gacgagcttg gcggaggcct
      961 gctccagccc gaacgcctcc agcagctcgt ccgcgcgcgc cttggcgccg ccgcgcaggc
     1021 ccagcagctt gcccaggagc atcaggttct cgcgcccggt caggtcctcg tcgaccgagg
     1081 ccagctggcc ggtcaggctg accttgtggc gcacggcgtc gccctcggtc gccacgtcgt
     1141 ggcccagcac cttcgcgctg ccgccgtcgg gggccaggag ggtggccagc atgcggatcg
     1201 tggtggtctt gcccgcgccg ttggggccca gcacgccgta cacggcaccg gtccgcacgg
     1261 ccaggtccac gccgtcgacg gccttcgtgt cgccgaagtg cttgaccagc ccggtggcct
     1321 cgatcgccag ttggtcggtc acgggggcaa cgtagcgcgg ccacgggccg gccagggtgc
     1381 ggctagcacg agagcttcgg gtaggcacaa tctgtccgtg gacttcacgt tcgaccccgg
     1441 cgcggccgcc gcgttgctgg tggccgaggc gctgtacgtg cgcgcctggc gcgtgctggg
     1501 cgggcgcggc taccgggtcc cgcgcggcca gctggcggcc tggcacgtgg gcctgctggc
     1561 catggtcgtc gccctgatgt cgcccatcgg cgggctcagc gacgagctgc tcgtggccca
     1621 catgggcgag cacctgatcc tggccgacct gggcgccccg ctgatgctgg tgggcatccg
     1681 ctcacccgtc tacgcgttcc tgctgcccgc cccggcgctc aagcggctgg cgcggcgcaa
     1741 gcggctacgc gccttcttcc gcacgctccg tcagccactc gtggccgtgc cgctgtggat
     1801 cctgatcctg tacggctggc acttcacgtt cctgttcgag aacgcgatcc gtcacgactg
     1861 ggtccacctt ctccagcacc agagcttcat cgtgggctcg ctgctggtct ggtggtcggt
     1921 gatcgagccc aagcgccgcc gcgccggcgg cgacctgtgg aaggtgccct acctgatcgg
     1981 cgcgcgcctg cccgggatgt tgctgggcat ggccttcatc atcatgggca gcgtcgccta
     2041 cgccgacccc tacgctgtga ctgcaccgca gcacgggtgg tccgccctga ccgaccagca
     2101 ggtcgccggg gggctgatga tggccacgga cctggccgtg atgctgttcg cgctgatgtt
     2161 cttcttctac cgctcagccc aggaccacga ccgcgccgag acggccgcgg cggtatgagg
     2221 cgcctggccg cggcatccgc gctggtgctg gccctcggcg cgggcggctg cagcctaggc
     2281 ggcgacgacg agggcggcgg gtccggcggc gccgaccgcg gcgcggccac cgccaccggc
     2341 ccggagatgc ccccgggcgt ggtcaagccc ggcgagccgc cggaggccgc ggcgatccgc
     2401 ggctggagcg aggccgtgaa cgcgcgccag ttcgggcggg cggcatcgtt cttcgcgcgc
     2461 gacgcgatcg tcgaccaggg ccaggccatc cgcctgcccg accgcagcgc cgccgcggcg
     2521 ttcagcaaga gcctgccctg caagtcccgg gtcacggaca tcaaggacga gggccgcacg
     2581 atcgtcgccg cgttcgacct gcggcccggc accgggccca agagcgcgtg cgacggcgac
     2641 gcgcgcgtgc ggttcacgtt ctcgggcaac aagttcaccg agttccgcca gctgatcgag
     2701 ccggagaccg ctccgggcca gagcgtctga tggaggtccg ccggctgggc cgcagcggcc
     2761 tgcgcgtctc cacgctgacg ctgggcacga tgggcttcgc cggcgccggc gggttcgccg
     2821 acatcggcga catcgacgtc gacggcgccc gccgccagct cgacctgtgc atcgacgccg
     2881 gcgtgaacct ggtcgacacg gccaacatgt actccgacgg cgcgtccgag gaggtcctgg
     2941 gcgaggcgct ggacggcggg cgccgcgaca agctgatggt ggcgaccaag gtccgcttcc
     3001 cgatgggcga cggcgccaac gacgccgggc tctcgcgccg acacatcatc gactcggtgg
     3061 aggcgtcgct gcgccgcctg ggcgccggcc acatcg
//
LOCUS       DASUPW010000242         2165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_609400, whole genome shotgun sequence.
ACCESSION   DASUPW010000242 DASUPW010000000
VERSION     DASUPW010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609400"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..591
                     /locus_tag="VFY44_05530"
     CDS             25..591
                     /locus_tag="VFY44_05530"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEX5909934.1"
                     /translation="MPFSNSPFRRAAEALEAKAALDAPGKPVRAVAGKLLSPAPVKAA
                     LSGSWLGHALHPILTDVVIGSFVSASVLDLVGGEDGEAGAERLLAVGIASYAPTALTG
                     VSDWADRAGDDAGVRRVGIVHAAGNAIALSLYTASLASRRAGSQQRGKLLALAGGAVM
                     GIGGYLGGHLAYVQRVGVETRPAPAAAS"
     gene            588..1646
                     /locus_tag="VFY44_05535"
     CDS             588..1646
                     /locus_tag="VFY44_05535"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5909935.1"
                     /translation="MKIVVTGASGNVGTSVLTALGADPGVREIVGLARRPPAGRTEKT
                     TWVPGDVADADLTTLFRGADAVIHLAWLIQPSRDQATLWRANVEGSARVFHAVAAAGV
                     PTLAYASSVGAYSPGPKDREVDEGWPTGGIDSSYYSRQKAEVERHLDRFEKEAPGCRV
                     VRLRPGLIFKRDAATEIRRYFAGPFLPDLLVRHRGLLPLLPLTTGLVFQAVHSLDVGE
                     AFRLAVTTDSAHGAFNVAADPVLDPARLAALVGARPVKVPAKVLRAAAAATWKARLQP
                     SSPGWLDMALMVPLMDTTRARQELGWTPRRTSEEALSELLDGMGHGAGYDTPPLSPDS
                     SGPFRFRELLSGVGARSR"
     gene            complement(1559..2059)
                     /locus_tag="VFY44_05540"
     CDS             complement(1559..2059)
                     /locus_tag="VFY44_05540"
                     /inference="COORDINATES: protein motif:HMM:NF020413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 domain-containing protein"
                     /protein_id="HEX5909936.1"
                     /translation="MADSSDPLERFVRAHDGGTYEHALSELRAGRKTSHWMWFVFPQI
                     EGLGRSAMAQRYAIASLEEARAYLAHPVLGPRLHECAEALLGQSGSSAEEILGGIDAV
                     KLRSSMTLFARADPGDPTFTRVLGRYFGGDADPATDQRLRAPTPESSSRNRNGPDESG
                     DSGGVS"
BASE COUNT          261 a          811 c          785 g          308 t
ORIGIN      
        1 gtgtgccggg taggcgctga accgatgccc ttctcgaact ccccattcag gcgcgccgcc
       61 gaggcactgg aggccaaggc ggctctggac gcccccggca agcccgtccg tgccgtcgcc
      121 ggcaagctgc tgtcgcccgc gccggtcaag gccgcgctca gcggaagctg gctcggacat
      181 gccctgcacc cgatcctgac cgacgtggtc atcggatcgt tcgtgagcgc ctcggtgctg
      241 gacctcgtcg gtggcgaaga tggcgaggcc ggggccgagc ggcttctggc ggtcggcatc
      301 gcgagctacg cgccgaccgc gctcaccggg gtcagcgact gggccgaccg ggccggcgac
      361 gacgcgggcg tgcgccgggt gggcatcgtg cacgccgccg gcaacgccat cgcgctttcc
      421 ctctacaccg cctcgctggc ctcccgccgg gccggttcgc agcagcgggg caagctgctg
      481 gccctggcgg gcggcgccgt gatgggcatc ggcggctacc tgggtggcca tctggcctac
      541 gtgcaacgcg tcggcgtgga gacgcggccg gccccggccg ccgcctcgtg aagatcgtcg
      601 tcacaggtgc gtcgggcaac gtcggcacca gcgtcctgac cgctctcggt gcggatcccg
      661 gcgtgcgcga gatcgtgggc ctggcgcgcc gtcctccggc cggccggacc gagaagacca
      721 cctgggtccc gggcgacgtg gcggacgccg acctgaccac gctgttccgc ggcgccgacg
      781 ccgtgatcca tctggcgtgg ctgatccagc cctcccgtga ccaggccacg ctgtggcgcg
      841 ccaacgtgga gggcagcgcc agggtcttcc acgccgtcgc ggccgccggg gtccccaccc
      901 tggcctacgc ctcctcggtc ggcgcctact cccccgggcc gaaggaccgt gaggtggacg
      961 aggggtggcc caccggcggc atcgactcct cgtactactc gcgccagaag gccgaggtcg
     1021 agcgccacct ggaccggttc gagaaggagg cccccggctg ccgcgtcgtg cgcctgcgcc
     1081 cgggcctgat cttcaagcgc gacgccgcca cggagatccg gcgctacttc gccgggccgt
     1141 tccttcccga cctgctcgtg cgccaccgcg ggctcctgcc cctgctgccg ttgaccaccg
     1201 gcctggtgtt ccaggcggtg cactcgctcg acgtcgggga ggcgttccgc ctggccgtga
     1261 ccaccgattc cgcccacggc gcgttcaacg tggcggccga cccggtgctc gacccggcgc
     1321 ggctggccgc cctcgtgggc gcccggccgg tcaaggtgcc cgccaaggtc ctgcgcgccg
     1381 ccgccgccgc cacgtggaag gcacgcctgc agcccagctc accggggtgg cttgacatgg
     1441 cgctgatggt cccgttgatg gacaccaccc gtgcgcggca ggagctcggc tggacgccgc
     1501 gccgcacgtc ggaggaagcg ctgtccgagc tgctcgacgg catgggccac ggcgcgggct
     1561 acgacacgcc gccgctgtcc ccggactcat ccgggccgtt ccggttccgt gagctgctct
     1621 cgggcgtcgg tgcgcgcagc cgctgatcgg tcgccggatc ggcatcgccg ccgaagtacc
     1681 gccccaggac ccgggtgaac gtggggtcgc cgggatccgc ccgggcgaag agggtcatcg
     1741 acgaccgcag cttcacggcg tcgatcccgc cgaggatctc ctcggcgctg gagcctgact
     1801 ggcccagcag ggcctccgcg cactcgtgga ggcgcgggcc caggacggga tgggccaggt
     1861 acgccctggc ctcctccagc gaggcgatgg cgtagcgctg cgccatcgcg ctgcgcccca
     1921 gtccctcgat ctgggggaag acgaaccaca tccagtggct ggtcttccgc cccgcgcgca
     1981 gctcgctcag ggcgtgctcg tacgtgccgc cgtcgtgcgc gcggacgaat cgttccagcg
     2041 gatccgagga gtcggccatc gatccttcct gaccggggag cacggtcgca aacgcgccgc
     2101 ccgggtgatt tccgcgccgc gggttggccg tgcggcggcc gggtagccga cggcgatggt
     2161 cgtcg
//
LOCUS       DASUPW010000243         2314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1441097, whole genome shotgun sequence.
ACCESSION   DASUPW010000243 DASUPW010000000
VERSION     DASUPW010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441097"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1292)
                     /locus_tag="VFY44_05545"
     CDS             complement(135..1292)
                     /locus_tag="VFY44_05545"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5909937.1"
                     /translation="MIDFELTDEQKLIRDTAREFADNEIAPRARENDREERFDHELVA
                     KMAELGFIGPILPEEYGGRGIDYRTYGLIVEEIGRVDSSARTVISVCTSLFGSAIARW
                     GTEEQKQRIIPGLTDGTGLGCFGLTEPDSGSDAASLKTRAEKTDGGWKLTGQKQWISL
                     GNVAKWALVFAQTDPEKAHKGLAAFVVPTDADGFSSSTIHGKLGLKASDTAELALDGV
                     EVGDDALLGEVGDGFKVAMTSLDSGRFSVASGCVGICQGCLDASVAYAKERTQFGRPL
                     AAFQLVQALIADMVLDTEAGRSLVWKAGWLKDQGRPNTLETSTAKLFATEAAIRTANN
                     AIQVHGGSGYVDDYPVERHLRDARVATLYEGTSQIQKLIIGRAMTGVNAMT"
     gene            complement(1289..>2314)
                     /locus_tag="VFY44_05550"
     CDS             complement(1289..>2314)
                     /locus_tag="VFY44_05550"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX5909938.1"
                     /translation="EDNRNVARMASLLAGFPVEVPGATVNRLCASGLEAVSQAARAVR
                     SDEGDLILAGGVESMSRAPMVTGKPERGFPRGGMELQDTTIGWRFVNPRMEELHGTDS
                     MGETAENVARRYDVSREDQDAFALESHRRAVAAAEAGFFDDELVTVEAPQPKGDPLAV
                     HADEGPRADSSLERLAKLRAIFREGGSVTAGNSSPINDGAACLVVASEERAGELGAEP
                     LARIVATGAAGVDPSYMGVGPIPATRLALRRAGLEMEDVDLVELNEAFASQSLACQRE
                     LGIDHEKLNVNGGAIAIGHPLGASGARLIGTLARELRRRGGRYGVATMCIGVGQGLAT
                     VIENPAA"
BASE COUNT          289 a          859 c          814 g          352 t
ORIGIN      
        1 gtcggcgtcg aagccctcga ggtcctcgac gatctcgcgc agcatgggcg cggtcagggt
       61 gttgcggctg gccgcgtggt cgatgcggat cagcccgacg gcgccgtcgc gctcggtggt
      121 gacggcggtc tccatcacgt catcgcgttc acgccggtca tggcgcgacc gatgatcagc
      181 ttctggatct gcgaggtgcc ctcgtagagc gtggccacgc gcgcgtcgcg caggtggcgc
      241 tcgaccgggt agtcgtccac gtagcccgag ccgccgtgca cctggatcgc gttgttggcg
      301 gtgcggatgg ccgcctcggt ggcgaacagc ttcgcggtcg aggtctccag cgtgttcggc
      361 ctgccctggt ccttgagcca gcccgccttc cacaccagcg agcggccggc ctcggtgtcc
      421 agcaccatgt cggcgatcag cgcctgcacc agctggaacg cggccagcgg gcggccgaac
      481 tgggtccgct ccttggcgta ggccaccgac gcgtccaggc agccctggca gatgcccacg
      541 cagcccgagg ccacgctgaa gcggcccgag tccagcgagg tcatggccac cttgaagccg
      601 tcgccgacct cgcccagcag ggcgtcgtcg cccacctcga ccccgtccag cgcgagctcg
      661 gcggtgtcgg aggccttgag gcccagcttt ccgtggatgg tgctcgagga gaagccgtcc
      721 gcgtcggtcg gcaccacgaa cgccgcgagc cccttgtggg ccttctcggg atcggtctgg
      781 gcgaagacca gcgcccactt cgccacgttg cccagcgaga tccactgctt ctggccggtc
      841 agcttccagc cgccgtcggt cttctcggca cgggtcttca gcgacgcggc gtccgagccg
      901 ctgtcgggct cggtcaggcc gaagcagccc aggccggtcc cgtcggtcag ccccgggatg
      961 atccgctgct tctgctcctc ggtcccccag cgggcgatgg ccgagccgaa cagcgacgtg
     1021 cacaccgaga tcaccgtgcg cgcggacgag tccacgcgcc cgatctcctc gacgatcagc
     1081 ccgtaggtgc ggtagtcgat gccgcggccg ccgtactcct cgggcaggat cgggccgatg
     1141 aacccgagct cggccatctt ggccaccagc tcgtggtcga agcgctcctc gcggtcgttc
     1201 tcgcgggcgc ggggcgcgat ctcgttgtcg gcgaactcgc gcgcggtgtc gcggatcagc
     1261 ttctgctcgt cggtcagctc gaagtcgatc atgccgcggg gttctctatc acggtggcca
     1321 ggccctgacc gaccccgatg cacatcgtcg ccacgccgta gcgcccgccg cgccggcgca
     1381 gctcgcgggc cagggtgccg ataaggcgtg cccccgaggc acccagcggg tggccgatgg
     1441 cgatggcgcc gccgttcacg ttcagcttct cgtggtcgat gccgagctcg cgctggcagg
     1501 ccagcgactg cgaggcgaac gcctcgttga gctcgaccag gtcgacgtcc tccatctcca
     1561 ggcccgcgcg ccgcagcgcc agccgggtgg cggggatcgg ccccacgccc atgtaggagg
     1621 gatccacgcc cgcggcgccg gtggccacga tgcgggccag cggctcggcg cccagctcgc
     1681 ccgcgcgctc ctcgctggcg accaccaggc acgctgcgcc gtcgttgatg ggggacgagt
     1741 tgccggcggt cacgctgccg ccctcgcgga agatcgcgcg cagcttggcc aggcgctcca
     1801 gcgaggaatc ggcccgtggc ccctcgtcgg cgtgcacggc cagcgggtcg cccttgggct
     1861 ggggcgcctc cacggtgacc agctcgtcgt cgaagaagcc cgcctcggcc gcggccaccg
     1921 cgcgccggtg cgactccagc gcgaacgcgt cctggtcctc gcgcgagacg tcgtagcgcc
     1981 gggccacgtt ctcggccgtc tcgcccatgg agtccgtgcc gtgcagctcc tccatccgcg
     2041 ggttgacgaa ccgccagccg atggtggtgt cctgcagctc catcccgccg cgggggaacc
     2101 cgcgctcggg cttgccggtg accatcgggg cgcggctcat ggactccacg ccgccggcca
     2161 ggatcaggtc gccctcgtcg ctgcgcacgg cgcgcgcggc ctggctgacg gcctcgaggc
     2221 cggacgcgca caggcggttg acggtggcgc cgggcacctc gaccgggaac ccggccagca
     2281 gcgacgccat ccgcgccacg ttgcggttgt cctc
//
LOCUS       DASUPW010000244         3873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_472654, whole genome shotgun sequence.
ACCESSION   DASUPW010000244 DASUPW010000000
VERSION     DASUPW010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472654"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VFY44_05555"
     CDS             complement(<1..555)
                     /locus_tag="VFY44_05555"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX5909939.1"
                     /translation="MTSLTESADGGTAPGGRVGNVDVLRAVAALMVLAGHAYLLSGRP
                     LGILADRWYDVLVLGGATGVWLFFAISGYVISKPFLDRLVTGQPLPALRGYALRRVVR
                     IFPLYVAALTAFIVIAGAGDIKPWHYPVHFLLLHNLVPGRETALLGVSWTLSIEVLFY
                     CAVPLLALAVRRWRPNVTAEWLAGA"
     gene            626..2353
                     /locus_tag="VFY44_05560"
     CDS             626..2353
                     /locus_tag="VFY44_05560"
                     /inference="COORDINATES: protein motif:HMM:NF023500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3556 domain-containing protein"
                     /protein_id="HEX5909940.1"
                     /translation="MGFLQPSPPPVDMDEWQRLPHLERIKPLAQDWALNGFGTPYAVY
                     LLYIVKLVIYVAGAIVVIGATTPGLGGIGDFGDWWTQPIVFQKLVVWTLLWEVVGLGA
                     GSMPLTFRFSPMIGGILYWLRPGTVRQPPWPERVPLTAGSTRTPVDVLLYAGLLAAGV
                     YLLVAGDGGTPVRLEPVAVGVTLALLGLLGLRDKLPFLAARAEVYGTLLVVCLFPLDN
                     LIVAAQLTFVGIWWGAASSKLNRHFPFVVSVMISNTPWNRSKFAKRRLYTNHPEDLRP
                     SREGALTAHLGTVIEFTLPLLLLVSKGGTIGTIAVVGMVIFHVHILSTFPLAVPLEWN
                     LFMIFGVLFLFGHYGDVPLSTLDNPLLMAILAVLLVGLPVLGNLRPDLISFLPSMRYY
                     AGNWATSQWLFRKETGAEETLDREMPKPAPIVVEQLTKFYGRDLAELLLAKGLAFRSM
                     HSHGRALNALHAHAVDDLEAYHVREGELISGVVCGYNFGDGHFHDHRLLAAVQERCGF
                     EPGDLRVICLESQPAHRKTQRYRILDAADGVLEEGEVDVVEMVSRQPWLGGEPFPVRV
                     DTPAATAAR"
     gene            2350..3768
                     /locus_tag="VFY44_05565"
     CDS             2350..3768
                     /locus_tag="VFY44_05565"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5909941.1"
                     /translation="MSTAVVVGSGPNGLACAALLATRGVSVTVIEAADTIGGGTRTSE
                     LTVPGLLHDDCSAAHPMGPGAPSLTSLGLEDHGLEWAWPEIDLAHPLDGGGAGVMVRS
                     IGDTAAGLGRDGRAWRAVFGSPSAHFDDLMADIMRPVQHVPAHPLRLLRFGIPAAAPA
                     TWLGQAFRTDEGRALFGGVAAHAFSPLNRPMSSSVAMALTSACHRFGWPVARGGSQAI
                     ADALASVVGAHGGRIETGRRVTSLDEVADADTVVLDLAPRGVADVAGDRLPRRVARAY
                     RRYRHGPGAFKVDLAVEGGVPWAAEPARRAGTVHAIGSFAETVAAERDVARGRMPERP
                     YVLVGQQYLADPSRSNGDTHPVWAYAHVPSGHAGDATEAVIDQLERFAPGLRERIVGQ
                     HTRSPAQLEAYNPNYVGGDIITGANTPVQTVIRPRVARDPYETGIPGVYICSAATPPG
                     AGAHGMNGYNAALSALRRLERA"
BASE COUNT          523 a         1395 c         1393 g          562 t
ORIGIN      
        1 cgcgccggca agccactcag ccgtgacgtt ggggcgccat ctccggacgg caagcgccaa
       61 gagcggcacg gcgcagtaga acagcacctc gatcgacagc gtccacgaga cccccagcag
      121 cgctgtctcg cgtcctggca cgaggttgtg aaggagcagg aagtggaccg ggtagtgcca
      181 cggcttgatg tcccccgcgc cggcgatgac gatgaacgcg gtcagcgccg ccacgtagag
      241 cgggaagatc cgaaccacgc gcctgagggc gtacccgcgc agggccggaa gcggctgtcc
      301 ggtcaccagg cgatccagga acggcttgct gatcacgtag cccgagatcg cgaagaacag
      361 ccacacgccg gtcgcgcccc cgaggaccag cacgtcgtac caccggtcgg ccaggatccc
      421 gagaggccgc ccgctgagca ggtacgcgtg cccggccaac accatcagcg ccgccacggc
      481 acgcaggaca tcgacattgc cgacccgccc gcccggcgcg gttcccccgt cggcggactc
      541 ggtgagggac gtcacggcgg caagcgtact tctcgtttcc cgttcaggcg caggtcaccg
      601 gcccaggtgg tagacacctc gctgcatggg cttcctccaa cccagccccc cgcccgtcga
      661 catggacgag tggcagcggc tccctcacct cgagcggatc aagcccttgg cccaggactg
      721 ggcgctgaac ggcttcggga cgccctacgc ggtgtacctg ctctacatcg tgaagctcgt
      781 gatctacgtc gccggggcga tcgtggtcat cggcgccacc acgccgggcc tggggggcat
      841 cggggacttc ggcgactggt ggacccagcc gatcgtgttc cagaagctcg tggtgtggac
      901 gctgctgtgg gaggtcgtgg gcctgggcgc ggggtcgatg ccgctgacgt tccggttctc
      961 gccgatgatc ggcgggatcc tgtactggct gcggccgggc accgtgcgcc agccgccgtg
     1021 gccggagcgc gttccgctga ccgccggcag cacccgcacg cccgtggacg tgctgctgta
     1081 cgccgggctg ctggccgcag gcgtgtacct gctggtggcg ggggacggtg ggaccccggt
     1141 ccgcctggag cctgtggccg tgggcgtgac gctcgccctg ctcggcctgc tgggcctgcg
     1201 cgacaagctc ccgttcctgg ccgcccgcgc cgaggtctac gggacgctgc tggtcgtctg
     1261 cctgttcccg ctggacaacc tgatcgtggc ggcgcagctc acgttcgtgg gcatctggtg
     1321 gggggccgcg tcgtccaagc tcaaccgcca cttcccgttc gtggtgtccg tgatgatcag
     1381 caacacgccg tggaaccggt ccaagttcgc caagcgacgg ctgtacacga accatcccga
     1441 ggacctgcgc ccgtcccgtg agggcgcgct gacggcccac ctggggacgg tgatcgagtt
     1501 caccctcccg ctgctgctcc tggtcagcaa gggcgggacc atcgggacca tcgcggtggt
     1561 cggcatggtc atcttccacg tgcacatcct gtccacgttc ccgctggccg taccgctgga
     1621 gtggaacctg ttcatgatct tcggcgtcct gttcctgttc ggccactacg gcgacgtgcc
     1681 gctgtcgacg ctggacaacc cgctgctgat ggccatcctg gccgtgctgc tggtggggct
     1741 gcccgtgctg ggcaacctcc ggccggacct gatctcgttc ctgccctcga tgcgctacta
     1801 cgccgggaac tgggcgacga gccagtggct gttccgcaag gagacggggg cggaggagac
     1861 cctggaccgt gagatgccca agcccgcccc gatcgtggtc gagcagctga ccaagttcta
     1921 cggccgcgac ctggccgagc tgctgctggc caagggcctg gccttccgct cgatgcactc
     1981 gcacgggcgc gccctgaacg ccctgcacgc gcacgcggtc gacgacctgg aggcctacca
     2041 cgtgcgcgag ggcgagctga tctccggggt ggtgtgcggc tacaacttcg gcgacgggca
     2101 cttccacgac caccggctgc tggcggcggt gcaggagcgc tgcggcttcg agcccggtga
     2161 cctgcgcgtg atctgcctgg agtctcagcc ggcgcaccgc aagacgcagc gctaccggat
     2221 cctggacgcc gccgacggcg tcctggagga aggggaggtc gacgtcgtgg agatggtctc
     2281 gcgccagccc tggctgggcg gcgagccgtt ccccgtgcgc gtggacaccc cggccgccac
     2341 agcggcccgg tgagcaccgc ggtcgtcgtc gggtccggcc ccaacggcct ggcctgcgcc
     2401 gccctgctgg ccacgcgcgg ggtgtcggtc acggtcatcg aggcggccga caccatcggt
     2461 ggcggcaccc ggaccagcga gctgaccgtg ccggggctgc tccacgacga ctgctcggcc
     2521 gcccatccga tgggccccgg agcgccgtcg ctgacctcgt tgggcctgga ggatcacggc
     2581 ctggagtggg cgtggcccga gatcgacctg gcccacccgc tcgacggcgg cggggcgggg
     2641 gtcatggtgc gctcgatcgg cgacacggcc gccggcctgg ggcgcgacgg ccgcgcctgg
     2701 cgagccgtgt tcgggtcgcc gtccgcgcac ttcgacgacc tgatggcgga catcatgcgc
     2761 cccgtccagc acgtgccggc ccacccgctg cgcctgctgc gcttcgggat cccggccgcc
     2821 gcgcccgcga cctggctggg tcaggccttc cgcaccgacg agggccgggc cctgttcggt
     2881 ggggtggccg cccacgcctt cagcccgctg aaccggccca tgagctcatc cgtggcgatg
     2941 gcgctgacca gcgcctgcca ccgcttcggc tggcccgtcg cgcgaggcgg ctcgcaggcc
     3001 atcgccgacg cgctcgcctc ggtggtgggc gcgcacggcg gcaggatcga gacgggccgg
     3061 cgggtcacat cgctcgacga ggtggccgac gcggacacgg tggtgctgga cctggcgccc
     3121 cgcggcgtcg ccgacgtggc cggcgaccgg ctgccgcggc gggtcgcgcg cgcctaccgt
     3181 cgctaccgcc acgggccggg cgcgttcaag gtggacctgg cggtcgaggg cggcgtgccc
     3241 tgggccgccg agccggcccg gcgggcgggc acggttcacg ccatcggctc cttcgccgag
     3301 accgtggccg cggagcgcga cgtggcgcgg gggcggatgc ccgagcggcc gtacgtgctg
     3361 gtgggccagc agtacctggc cgacccgagc cggtcaaatg gcgacaccca cccggtgtgg
     3421 gcgtacgcgc acgtgcccag cggccatgcc ggggacgcca cggaggcggt catcgaccag
     3481 ctcgagcggt tcgctcccgg actgcgtgaa cggatcgtcg gccagcacac ccggtcgccg
     3541 gcgcagctgg aggcctacaa ccccaactac gtcggcggcg acatcatcac cggcgccaac
     3601 acccccgtgc agacggtgat ccgcccgcgc gtggcgcggg atccgtacga gaccggcatc
     3661 ccgggggtct acatctgctc ggccgccacg cctcccggtg cgggagcgca cgggatgaac
     3721 ggctacaacg ccgcattgtc tgcgctgcgc cggctcgaac gggcctgacg ctcaagcact
     3781 cctccgtccg gtcgatagct cgggcatggt cagcgaatcc cgtgacgagt gccccggctg
     3841 cagcgcggcc ggaccgtgct gctgcggctg gca
//
LOCUS       DASUPW010000245         3406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_748209, whole genome shotgun sequence.
ACCESSION   DASUPW010000245 DASUPW010000000
VERSION     DASUPW010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748209"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VFY44_05570"
     CDS             <1..308
                     /locus_tag="VFY44_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909942.1"
                     /translation="YSTEAGGDEGMTPAEVAEEAKEGYYDAQFGVPGGSYETYLRGLA
                     TARNPSELLAFVQNSSFGNSSSVVDDLLRLQMQPIGEDGRPEPRTGSLPGSIDVYDD"
     gene            complement(310..621)
                     /locus_tag="VFY44_05575"
     CDS             complement(310..621)
                     /locus_tag="VFY44_05575"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEX5909943.1"
                     /translation="MSGQWAALVAGCAVMAFVIKSVGPVVFGGRPLPERLRAVVFLVA
                     APLLAALVVTQAMAEGDELKLDAETAGVALAGVAGWFRVPSTICLLLAAAVTGGLRAL
                     G"
     gene            complement(618..1304)
                     /locus_tag="VFY44_05580"
     CDS             complement(618..1304)
                     /locus_tag="VFY44_05580"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEX5909944.1"
                     /translation="MNASFRRGVKEGLPFAVAGGLLSISFGVLANDTGLGAWGAILMS
                     AVVFGGSAQFASVGILSTGGSLGTALLAATLVNSRFLPMGVALAPYLPGGPLKRALQG
                     QPLVDPSWVLARDTDGAFDRHVLFGASAAQYVTWVSGTVLGAFVGTGAIEPDSLGVDA
                     MFPAFFLILLLEELRQPGMRGVALGGALIAVVLVPLTPIGVPVLVASGAALVGLRRGP
                     HEAAPDWGET"
     gene            complement(1301..1669)
                     /locus_tag="VFY44_05585"
     CDS             complement(1301..1669)
                     /locus_tag="VFY44_05585"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5909945.1"
                     /translation="MIEAAGGVVVRGDRVAVVHRPRYDDWSLPKGKLDEGETFEEAAL
                     REVQEETGLVCELGDELPSTEYHDRKGRPKVVRYWRMTVVQDPGFEPNDEVDELRWLP
                     AAEATPLLSYEHDRKLLEGL"
     gene            complement(1666..2517)
                     /gene="thrB"
                     /locus_tag="VFY44_05590"
     CDS             complement(1666..2517)
                     /gene="thrB"
                     /locus_tag="VFY44_05590"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX5909946.1"
                     /translation="MNRHRIVRAPASSANLGPGYDVLAAALSLELELEVEETGEFSVV
                     SDTDGVPLDRTNLCVRAFEKLHSADGLAFSIRSQIPPAGGLGSSAACIVAGLAAADHM
                     FELDAPLFEPACELEGHPDNVAAALYGGFVICGPGDPVRIEPSEGLEGIIARPPAPVS
                     TEQARAALPDQVPMTEASFNIGHASLLTLGLARSDFSLIGRGLDDRLHQPRRAALYPR
                     SIELLHRAREFGAIGATISGAGPSVLFWCQWQTTGDVQSALKAAAPDCEVQRVNFAPG
                     GVDVRDL"
     gene            complement(2514..>3406)
                     /gene="thrC"
                     /locus_tag="VFY44_05595"
     CDS             complement(2514..>3406)
                     /gene="thrC"
                     /locus_tag="VFY44_05595"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013085328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX5909947.1"
                     /translation="GCDVYLKVEGANPTGSFKDRGMTVAVSAAIGEGAEAVICASTGN
                     TAASLAAYAARAGLRGAVIVPEGKIATGKLAQALMHGARVIALSGNFDEALALVRQLA
                     DKHPIALVNSVNEYRIEGQKTSAFEICDELGEAPDALCIPVGNAGNVTAYWRGFEEYG
                     VRPRLYGFQAEGAAPLVLGAPVAAPETVASAIRIGNPARWEEAMGAFTGSRGQVRAVS
                     DQQILDAYDLLAAREGVFCEPASAASVAGLLAHGAEGRVVCVLTGHGLKDPQTAMNRA
                     SSVIPCRPDIASVEAAVLEP"
BASE COUNT          494 a         1313 c         1197 g          402 t
ORIGIN      
        1 actactcgac cgaggccggc ggcgacgagg gcatgacgcc ggccgaggtg gcggaggagg
       61 ccaaggaggg ctattacgac gcccagttcg gcgtccccgg cggctcgtac gagacctacc
      121 tgagggggct ggccacggcg cgcaacccca gcgagctcct ggcgttcgtg cagaactcga
      181 gcttcggcaa ctcctcttcg gtggtcgacg acctgctgcg gctccagatg cagccgatcg
      241 gcgaggacgg gcgccccgag ccccggacgg gctcgctccc gggctccatc gacgtctacg
      301 acgactgaat cagcccaggg cgcgcaggcc gccggtgacg gcggcggcga gcagcaggca
      361 gatggtggac ggcacgcgga accagcccgc gacccccgcg agcgccacgc ccgccgtctc
      421 ggcgtcgagc ttgagctcgt cgccctcggc catcgcctgg gtgaccacca gcgccgccag
      481 cagcggcgcc gccaccagga acaccaccgc gcgcaggcgc tcgggcagcg ggcggccgcc
      541 gaagaccacc ggccctaccg acttgatcac gaaggccatg accgcgcagc cggccacgag
      601 cgccgcccac tggccgctca cgtctcaccc cagtcgggcg cggcctcgtg cgggccccgg
      661 cgcaggccca ccagggccgc cccgctggcc accagcaccg gcacgccgat gggggtcagg
      721 ggcaccagca cgacggcgat cagcgcgccg ccgagcgcga cgccgcgcat ccccggctgg
      781 cgcagctcct ccagcagcag gatcaggaag aacgcgggga acatggcgtc cacgcccagg
      841 ctgtcgggct cgatggcgcc ggtgcccacg aacgcgccga gcaccgtgcc cgagacccag
      901 gtcacgtact gcgccgcgct ggcgccgaac aggacgtggc ggtcgaacgc gccgtccgtg
      961 tcgcgggcca ggacccagga cgggtccacc agcggctggc cctggagcgc tcgcttgagc
     1021 ggcccgccgg gcaggtaggg cgccagggca acccccatcg gcaggaaccg cgagttcacc
     1081 agcgtcgcgg ccagcagcgc ggtgcccagg ctcccgccgg tggacaggat gcccacggag
     1141 gcgaactgcg ccgagccgcc gaacaccacc gcggacatca ggatcgcgcc ccacgcgccc
     1201 agcccggtgt cgttggccag cacgccgaag gagatggaca gcagcccgcc ggcgacggcg
     1261 aacggaaggc cttcctttac cccccgccgg aaagaggcgt tcacaaccct tccagcagct
     1321 tgcgatcgtg ctcgtaggag agcagcggcg tggcctccgc cgcgggcagc caccgcagct
     1381 cgtcgacctc gtcgttgggc tcgaagcccg ggtcctgcac cacggtcatc cgccagtagc
     1441 gcacgacctt cggccggccc ttgcggtcgt ggtactccgt cgagggcagc tcgtcgccga
     1501 gctcgcacac caggccggtc tcctcctgca cctcgcgcag cgcggcctcc tcgaaggtct
     1561 cgccctcgtc cagcttgccc ttgggcagcg accagtcgtc gtaacggggg cggtgcacca
     1621 ccgccacgcg gtcgccgcgg accaccacgc cacccgccgc ctcgatcaca ggtccctcac
     1681 gtccacgccg ccgggcgcga agttcacgcg ctgcacctcg cagtcgggcg ccgccgcctt
     1741 gagcgccgac tgcacgtcac ccgtggtctg ccactggcac cagaacagga ccgaggggcc
     1801 cgcgcccgag atcgtggccc cgatggcgcc gaactcccgt gcgcggtgca ggagctcgat
     1861 cgaacgcggg tacagggccg cgcggcgcgg ctggtgcagc cggtcgtcca ggccgcgccc
     1921 gatcagggag aagtccgatc gcgccagccc gagcgtcagc agcgaggcgt ggccgatgtt
     1981 gaaggaggcc tcggtcatcg gcacctggtc gggaagggcg gcgcgggcct gctcggtgga
     2041 caccggcgcg ggcgggcggg cgatgattcc ctccaggccc tcggagggct cgatccgcac
     2101 cgggtcgccc gggccgcaga tcacgaagcc gccgtacagc gcggcggcca cgttgtcggg
     2161 gtgtccctcc agctcgcagg cgggctcgaa cagcggcgcg tccagctcga acatgtggtc
     2221 cgcggcggcc aggccggcga cgatgcaggc agcgctggag ccgaggcccc ccgcgggcgg
     2281 gatctgcgac cggatcgaga acgcgagccc gtcggcgctg tggagcttct cgaacgcgcg
     2341 cacgcacagg ttggtccggt ccagcggcac cccgtcggtg tccgagacca ccgagaactc
     2401 gccggtctcc tccacctcca gctccagctc cagcgacagg gccgccgcca gcacgtcgta
     2461 cccgggcccc aggttggccg aggacgcggg cgcgcggacg atccggtgcc ggttcacggc
     2521 tccagcaccg cggcctccac gctggcgatg tcgggccggc acgggatcac cgaggacgcg
     2581 cggttcatgg ccgtctgcgg gtccttcagg ccgtggccgg tcagcacgca caccacacgg
     2641 ccctcggcgc cgtgggccag cagcccggcg accgacgccg cggacgcggg ctcgcagaac
     2701 acgccctcac gcgcggccag caggtcgtag gcgtccagga tctgctggtc gctgaccgcg
     2761 cgcacctggc cgcgcgagcc ggtgaaggcg cccatggcct cctcccaccg ggccgggttg
     2821 ccgatcctga tcgccgaggc caccgtctcc ggggccgcga ccggcgcgcc gagcaccagc
     2881 ggcgccgccc cctcggcctg gaagccgtag agcctcgggc gcacgccgta ctcctcgaag
     2941 ccgcgccagt acgccgtcac gttgcccgcg ttgccgaccg ggatgcacag ggcgtcgggc
     3001 gcctccccca gctcgtcgca gatctcgaac gccgacgtct tctgaccctc gatccggtac
     3061 tcgttgaccg agttgaccag cgcgatcggg tgcttgtcgg ccagctggcg caccagcgcc
     3121 agcgcctcgt cgaagttccc gctgagcgcg atcacccggg cgccgtgcat cagcgcctgg
     3181 gccagcttgc cggtggcgat cttgccctcg gggacgatca cggcgccccg caggccggcg
     3241 cgtgccgcgt acgcggccag gctggccgcg gtgttgccgg tggaggcgca gatcaccgcc
     3301 tcggccccct cgccgatggc ggccgacacg gccacggtca tcccgcggtc cttgaagctg
     3361 cccgtggggt tggcgccctc gaccttcagg tacacgtcgc acccgg
//
LOCUS       DASUPW010000246         6214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_195314, whole genome shotgun sequence.
ACCESSION   DASUPW010000246 DASUPW010000000
VERSION     DASUPW010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6214
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195314"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VFY44_05600"
     CDS             <1..214
                     /locus_tag="VFY44_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEX5909948.1"
                     /translation="GEDFGTGRTIGEVELLDTRTRRWRRLPDMRTPRHGLGGVARANR
                     VYSLLGGPRPGLHFSSTTEFLDVRAP"
     gene            211..732
                     /locus_tag="VFY44_05605"
     CDS             211..732
                     /locus_tag="VFY44_05605"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5909949.1"
                     /translation="MIPLKDDIPARRPPVVTFVAIAAAALAWLVAGDRLGVDGGGWTL
                     LVAAAFLWLFAPSVEDAMGQARFTVFLVAGGALTWALDPAAAVAGSVAAVLGAYAVLY
                     PRAHVVALNLVPGFMTVTQVPAPAVLVLWLPLQALAGGLDPAPLAGLAAGAAAARPLA
                     HRVQHDYERAPAF"
     gene            732..911
                     /locus_tag="VFY44_05610"
     CDS             732..911
                     /locus_tag="VFY44_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909950.1"
                     /translation="MPGRNAALVATLALIALLGYLTVNVLIREGFDILVATSLLILAM
                     FSFGVVGALLNPPEE"
     gene            908..1894
                     /locus_tag="VFY44_05615"
     CDS             908..1894
                     /locus_tag="VFY44_05615"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX5909951.1"
                     /translation="MSGPLRGGRRRRRLVPALLVLFVLALPVAAAAGAYLWVTADDGP
                     GAQPEVVDTPSVVPEEGGSLEARSPAPVRLTGGNAFDPRFRKAPRAALVFDVDDGRVL
                     IRREPERVLPMASLTKIMTALAVTSETRPDEKVRITPAALKYQGSGVGVLPKGKRVPL
                     EALMNGLMLVSGNDAAIALADHVSGSERQFVRLMNEKARLWGLRCTRFASSHGLEKGN
                     RSCAADLAVMTRLAMRNPRITGIVRRKEASFRFPIKGGKLYLYGHNPLIRAGYRGAIG
                     LKTGYTDEAGRCFVGVARRDGRTLGVVLLHSPNPLKHATALLDQGFRAEGAR"
     gene            1914..1986
                     /locus_tag="VFY44_05620"
     tRNA            1914..1986
                     /locus_tag="VFY44_05620"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1947..1949,aa:Ala,seq:tgc)
     gene            complement(2165..2788)
                     /locus_tag="VFY44_05625"
     CDS             complement(2165..2788)
                     /locus_tag="VFY44_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909952.1"
                     /translation="MGLLDDAIRQHLELKRAHGADEAELKRKEDEALGPVRREAEPAA
                     EGGGEDAAAPEQEPVADAPTAPAAEEPFAEEPFAEAPVAEAPPAEEPVAQEPAAFADD
                     DPFADDEPFADEPAPVAEEPAAEEPPPRVSNTPTRGFDALSEEELEDEPFADEPAPPP
                     APAEPPAGDAEDVLEETPEFLQETPEHDRLWFEQKPPRDFDFDDEEK"
     gene            2878..4092
                     /gene="fdhA"
                     /locus_tag="VFY44_05630"
     CDS             2878..4092
                     /gene="fdhA"
                     /locus_tag="VFY44_05630"
                     /EC_number="1.2.1.46"
                     /inference="COORDINATES: protein motif:HMM:TIGR02819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018467 - formaldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde dehydrogenase,
                     glutathione-independent"
                     /protein_id="HEX5909953.1"
                     /translation="MPDNRGVAYIGAGKVEVQNIDFPELELKDGPGVHPDNVGRKTPH
                     GAILKIVSTNICGSDQHMVRGRTTAPENLILGHEITGEVIETGPGVEFIKEGDLVSVP
                     FNIACGRCTMCKDGHTGVCLNTNPDRPGSAYGYVDMGGWVGGQAEYVMVPYADWNLLK
                     FPDKDQAMEKILDLTMLSDIFPTGYHGCVTAGVTTGSSVYIAGAGPVGLAAAHSAQLL
                     GAAVVIVGDMQTQRLDQAKAFGCEVIDLNKATPEEGIEQILGEPVVDAAVDCVGFEAR
                     GHGEGAGEAPATVLNSLMSVTRAAGKLGIPGLYVTGDPGAVDDAAKEGSLSVAIGVGW
                     AKSLSFTTGQCPVMRYHRQLMNSILHGKAEIAKAVNATVITLDEAPKGYEDFDKGAAQ
                     KFVLDPHGLVAA"
     gene            complement(4097..4936)
                     /locus_tag="VFY44_05635"
     CDS             complement(4097..4936)
                     /locus_tag="VFY44_05635"
                     /inference="COORDINATES: protein motif:HMM:NF014951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909954.1"
                     /translation="MSTVHLLTGESMPKPDPETHLLTAELESRGVGHRVLPWTDPDAG
                     RGADLVVVRTPWDYIHRCDEFLRVCRAAEPPVLNPPDVLAWNSHKSYLVELAEAGVPV
                     VPTRLVRAGEAAALAADEAEVALVVKPAVSSGSRGAGRFEPGDPAAAAHLADLVEDAD
                     ALVQPYVASVAERGERSLIFLGGEFSHAVDKVPAQGDYRVQEMYGGRYRAHVPTRAER
                     AAAERALAAAPSAQELLFARVDLVDGAGGPEVMELELIEPELFLGFEAAAPRRLADLI
                     AAS"
     gene            complement(4933..>6214)
                     /locus_tag="VFY44_05640"
     CDS             complement(4933..>6214)
                     /locus_tag="VFY44_05640"
                     /inference="COORDINATES: protein motif:HMM:NF014998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX5909955.1"
                     /translation="AGPSVSAGGVPLEEADRTILALEGPTVVGHTCKVVLVGPGAPAV
                     PELRESIAARLDAAPALTRRLGGPGDAPEWVPDPGFDVSEHVVAAGDGAPVARSRLRD
                     EVARLFAQHLDRDRPLWRIDVLALDDGATALVWRLHHALADGTASMRLARGMLWDAVP
                     AGEPLPDQPHTAAAHSADEERRQAHLAAFLRREFARSAGRSPFDGHIGRRRQIAFASV
                     SLKRLHDAGRALAGATLNDSVLAVVAGALRAWVQHHHGDLGGVRVRVPVSLHHEGDDA
                     GNRDSFFTVALPLNEPDPVARLCAVHDATAVRKADHDAEEMDQLLQGLAGVSPRLSRF
                     CERIEGSPRHFAVNVSNVPGPRTPVSVLGAPVESLHSVAEIGERHALRITAISLAGTL
                     CFGFCADPDLVDDVQAMADGVEQGAAELDRLAGV"
BASE COUNT          811 a         2282 c         2293 g          828 t
ORIGIN      
        1 cggcgaggac ttcgggaccg ggcgcacgat cggcgaggtg gagctgctgg acacccgtac
       61 gcgccgctgg aggcggctcc cggacatgcg caccccgcgc cacggcctcg gcggcgtggc
      121 acgcgccaac cgcgtctact cgctgctggg cgggccccgg cccggcctgc acttctcctc
      181 caccaccgag ttcctggacg tgcgggcgcc gtgatcccgc tcaaggacga catccccgcc
      241 cgccgtcccc cggtggtcac cttcgtggcc atcgcggccg ccgcgctggc ctggctggtg
      301 gccggcgacc ggctgggcgt ggacgggggc ggatggaccc tcctggtggc cgccgcgttc
      361 ctgtggctgt tcgccccgtc ggtggaggac gcgatggggc aggcgcggtt caccgtgttc
      421 ctcgtggccg gcggcgcgct gacgtgggcg ctcgaccccg ccgccgcggt ggccgggtcg
      481 gtggcggcgg tcctgggcgc ctacgccgtg ctgtaccccc gcgcgcacgt ggttgcgctg
      541 aacctggtgc cggggttcat gaccgtcacc caggtcccgg cgccggccgt gctggtcctg
      601 tggctgccgc tccaggcgct ggccggcggg ctcgaccccg cgccgctggc cgggctggcc
      661 gccggcgcgg ccgccgcgcg cccgctggcc cacagggtgc aacacgacta cgagcgcgcg
      721 cccgccttct gatgcccggc cggaacgccg cgctcgtggc tacgctggcg ctgatcgccc
      781 tgctgggcta cctgaccgtg aacgtcctga tccgagaagg cttcgacatc ctggtggcca
      841 cgtccctgct gatcctggcc atgttcagct tcggcgtggt gggcgccctg ctcaacccgc
      901 ccgaggaatg agcgggccgc tccggggagg ccggcggcgc cggcggctgg tgcccgccct
      961 gctggtgctg ttcgtcctgg ccctgccggt ggccgcggcg gcgggcgcct acctgtgggt
     1021 gaccgccgac gacggcccgg gcgcccagcc cgaggtggtc gacacgccca gcgtggtgcc
     1081 cgaggagggc gggtcgctgg aggcgcgctc gcccgccccg gtgcgcctga ccggcggcaa
     1141 cgcgttcgat ccccggttcc gcaaggcgcc ccgggccgcg ctggtgttcg acgtggacga
     1201 cgggcgcgtg ctgatccggc gcgagcccga gcgcgtgctg cccatggcca gcctgaccaa
     1261 gatcatgacg gcgctggccg tgacctccga gacccggccc gacgagaagg tccggatcac
     1321 ccccgctgcg ctgaagtacc agggctccgg cgtcggggtg ctgcccaagg gcaagcgcgt
     1381 gccgctggag gcgctgatga acgggttgat gctggtgtcg ggcaacgacg ccgccatcgc
     1441 cctggccgac cacgtctcgg gcagcgagcg ccagttcgtt cggctgatga acgagaaggc
     1501 gcggctgtgg ggcctgcgct gcacccggtt cgcctcctcg cacgggctgg agaagggcaa
     1561 ccgctcctgc gccgccgacc tggcggtgat gacccgcctg gccatgcgca acccgcgcat
     1621 caccggcatc gtgcggcgca aggaggccag cttccgcttc ccgatcaagg gcggcaagct
     1681 ctacctgtac ggccacaacc ccctgatccg cgccggctac cgcggcgcca tcgggctcaa
     1741 gacgggctac accgacgagg cgggccgctg cttcgtgggc gtggcccggc gcgacggccg
     1801 gaccctgggc gtggtgctgc tgcactcgcc gaacccgctc aagcacgcca ccgcgctgct
     1861 ggaccagggg ttcagggccg agggcgcgcg ctgagtccta ccatgacgtc tgcggggcca
     1921 tagctcagct ggaagagcgt cgcgtttgca acgcgaaggt cgccggttcg aacccggctg
     1981 gctccactgg agtgctggac gtttgttgga cgcccggccc ccgcgagtaa agacccctcc
     2041 tcccgtgcgt cgagacccga tgtacgacgg cacggagggt tccgtcgaca tagcggcacg
     2101 ggacgccgcg aaacatgaga cggggatcgc cggggcgggc ggtccccgtt tctgtcttcg
     2161 ggagctactt ctcttcgtcg tcgaagtcga agtcgcgtgg cggcttctgc tcgaaccaga
     2221 gccggtcgtg ctccggcgtc tcctggagga actcgggcgt ctcctcgagc acgtcctcgg
     2281 cgtcgccggc cgggggctcg gcgggcgcgg gcggcggtgc gggctcgtcg gcgaacggct
     2341 cgtcctcgag ctcctcctcc gacagcgcgt cgaacccgcg cgtgggcgtg ttgctcaccc
     2401 tgggcggcgg ctcctcggcc gcgggctcct cggccacggg ggccggctcg tcggcgaacg
     2461 gctcgtcatc ggcgaagggg tcgtcgtcgg cgaacgcggc cggctcctgg gcgaccggct
     2521 cctcggccgg cggcgcctcg gccacggggg cctcggcgaa cggctcctcg gcgaagggct
     2581 cctcggccgc gggcgccgtg ggcgcgtcgg cgacgggctc ctgctccggc gcggcggcgt
     2641 cctcgccgcc cccctcggcg gcgggctccg cttcgcgtcg caccggtccc agcgcctcgt
     2701 cctccttgcg cttgagctcg gcctcgtcgg ccccgtgcgc gcgtttgagt tccaggtgct
     2761 gccggatggc gtcgtcgaga agacccatcg gcgcgaagcc taatagatcc gcccgaaggt
     2821 tccgccgcgt gggcatcggg cacccaacag gtagaggaga gcgaaggagg agcgagcatg
     2881 cctgacaacc gcggcgtggc ctatatcggt gccggcaagg tcgaggtgca gaacatcgac
     2941 tttcccgagc tggagctgaa ggacggcccc ggcgtccatc ccgacaacgt gggccgcaag
     3001 acgccacacg gggcgatcct gaagatcgtc tcgaccaaca tctgcggatc ggaccagcac
     3061 atggtccgcg gccgcaccac cgcccccgag aacctgatcc tggggcacga gatcaccggc
     3121 gaggtcatcg agaccgggcc cggcgtggag ttcatcaagg agggcgacct ggtctcggtc
     3181 cccttcaaca tcgcctgcgg gcgctgcacc atgtgcaagg acggccacac cggcgtgtgc
     3241 ctgaacacca acccggaccg gcccggatcg gcgtacgggt acgtggacat gggcggctgg
     3301 gtcggaggcc aggcggagta cgtgatggtc ccgtacgccg actggaacct gctcaagttc
     3361 cccgacaagg accaggcgat ggagaagatc ctggacctga cgatgctgtc ggacatcttc
     3421 cccaccggct accacggctg tgtgacggcc ggggtgacca cggggtcgtc ggtctacatc
     3481 gccggcgcgg gcccggtggg cctggccgcg gcgcactcgg cgcagctgct gggcgccgcc
     3541 gtggtgatcg tcggcgacat gcagacccag cgcctggacc aggcgaaggc gttcggctgc
     3601 gaggtcatcg acctgaacaa ggccacgccg gaggagggca tcgagcagat cctgggcgag
     3661 cccgtcgtgg acgcggccgt ggactgcgtg ggcttcgagg cccgcgggca cggtgagggc
     3721 gccggtgagg cgccggcaac ggtgctgaac tcgctgatga gcgtgacccg ggccgccggc
     3781 aagctgggca tcccgggcct gtacgtgacg ggtgacccgg gcgccgtcga cgacgcggcg
     3841 aaggagggct cgctctcggt cgcgatcggc gtgggctggg cgaagtcgct gtcgttcacc
     3901 accggccagt gcccggtgat gcgctaccac cgccagctga tgaactcgat cctgcacggc
     3961 aaggcggaga tcgccaaggc cgtgaacgcc acggtgatca ccctggacga ggccccgaag
     4021 ggctacgagg acttcgacaa gggcgcggcc cagaagttcg tgctggaccc gcacggtctg
     4081 gtggccgcct gaacggtcag gacgcggcga tcagatcggc cagccggcgc ggcgcggcgg
     4141 cctcgaagcc gaggaagagc tcgggctcga tgagctcgag ctccatgacc tcggggccgc
     4201 cggcgccgtc gaccaggtcc acgcgggcga agagcagctc ctgcgccgac ggcgccgccg
     4261 ccagggcccg ctcggccgcc gcccgctcgg cccgggtcgg gacgtgggcc cggtagcgcc
     4321 cgccgtacat ctcctgcacc cggtagtcgc cctgcgcggg caccttgtcg accgcgtgcg
     4381 agaactcccc gcccaggaag atcagcgacc gctcgccgcg ctcggccacc gaggccacgt
     4441 agggctggac gagcgcatcg gcatcctcga ccaggtcggc caggtgggcg gccgcggccg
     4501 ggtcgccggg ctcgaaccgc cccgcgccgc gcgagccgct ggacacggcg ggcttgacca
     4561 ccagcgccac ctcggcctcg tccgccgcca gcgccgccgc ctcaccggcg cggaccagcc
     4621 gcgtgggcac caccggcacg ccggcctccg caagctccac caggtacgac ttgtggctgt
     4681 tccaggccag cacgtccggc gggttcagga cgggcggctc cgcggcccgg cacacccgta
     4741 ggaactcgtc gcagcggtgg atgtaatccc agggggtacg gaccaccacg aggtccgcgc
     4801 cgcggccggc gtccgggtcg gtccagggca gcacacggtg ccccacccct ctcgactcga
     4861 gctccgccgt cagcaggtgg gtctccgggt cgggcttggg catcgactcg ccggtgagaa
     4921 ggtggaccgt gctcatacgc ccgccagccg gtccagctcg gcggcgccct gctccacgcc
     4981 gtcggccatg gcctgcacgt cgtccaccag gtcggggtcg gcgcagaagc cgaagcacag
     5041 cgtcccggcc agcgagatgg cggtgatgcg cagcgcgtgg cgctcgccga tctcggccac
     5101 cgagtgcagc gactccaccg gcgcgcccag cacgctgacc ggcgtgcgcg ggccggggac
     5161 gttggacacg ttcacggcga agtggcgcgg gctgccctcg atgcgctcgc agaaccgcga
     5221 caggcgcggc gacacgcccg cgagcccctg gagcagctgg tccatctcct cggcgtcgtg
     5281 atcggccttg cgcaccgcgg tcgcgtcgtg caccgcgcac aggcgggcca ccgggtcggg
     5341 ctcgttcagc ggcagcgcca ctgtgaagaa ggagtcgcgg ttgccggcat cgtcgccttc
     5401 gtggtgcagg ctgaccggaa cccgcacgcg gacgccgccg aggtcgccgt ggtggtgctg
     5461 cacccacgcc cgcagggcgc ccgcgaccac ggccagcacc gagtcgttca gggtcgcgcc
     5521 ggccaacgcg cggcccgcgt cgtgcagccg tttgagggac accgacgcga acgcgatctg
     5581 gcgccgccgg ccgatgtgcc cgtcgaacgg cgaccgcccc gccgagcgcg cgaactcacg
     5641 ccgcaggaag gcggccaggt gggcctgccg gcgctcctcg tcggcggagt gggcggccgc
     5701 cgtatgcggc tggtcgggca gcggctcgcc ggccggcacc gcgtcccaca acatcccgcg
     5761 ggccaggcgc atgctggccg tgccgtcggc cagcgcgtga tgcagacgcc acaccagcgc
     5821 ggtggcgccg tcgtccagcg ccaggacgtc gatgcgccac aggggccggt cgcggtccag
     5881 gtgctgcgcg aacaggcggg ccacctcgtc acgcagccgc gaccgggcga cgggcgcgcc
     5941 gtcgcccgcg gccaccacgt gctcggacac gtcgaagccc gggtcgggaa cccattcggg
     6001 cgcatccccg ggaccgccca gccgccgggt cagggccggc gccgcgtcca gccgggcggc
     6061 gatcgactcg cgcagttccg gcacggcggg cgcgcccggg cccacaagca ccaccttgca
     6121 cgtgtggccc accaccgtcg ggccctccag ggccaggatc gtccggtcgg cctcctccag
     6181 cgggacgccg ccggcgctca cgctcgggcc tgct
//
LOCUS       DASUPW010000247         2802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1442099, whole genome shotgun sequence.
ACCESSION   DASUPW010000247 DASUPW010000000
VERSION     DASUPW010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442099"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1165)
                     /locus_tag="VFY44_05645"
     CDS             complement(149..1165)
                     /locus_tag="VFY44_05645"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX5909956.1"
                     /translation="MPVLAHDELLQRQAAVSAVVVLGLTALWGLTGGGVFWPGWVAFG
                     LLLLMALVVSLRWAWAQTPGLTRRLAVQGAVSAVLVVMEIGLWAGTGFGPLWPYWSAL
                     GYSVALAAHWLIAVLWFQMHPEREQKLNRRVEELTRTRRGALDVEAAELRRIERDLHD
                     GAQARLVALSMQLGRAEERLGDQPEVAAEFRRARDEASAVIGELRDLARGIAPPVLAD
                     RGLPAAVEALGRRAPIPVTVTAQLAGRPAPVIETAAYFVVAEALTNAVKHGGGAPAHV
                     TVSQLGDRLTAAVRDEGPGGAQVDLGGGLEGLRARVEALDGTLVVSSAAGAGTTVTAE
                     LPCA"
     gene            complement(1243..2127)
                     /gene="rapZ"
                     /locus_tag="VFY44_05650"
     CDS             complement(1243..2127)
                     /gene="rapZ"
                     /locus_tag="VFY44_05650"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX5909957.1"
                     /translation="MAEIRTRLRDLVVITGYSGAGKSTAMNVFEDAGYFCVDNLPPEM
                     IRGLVDLFAHEGSKVERAAVVCDVRGGTYFESLADAVDSLQAADLPHRILFLDAAEDT
                     LLDRYKETRRRHPLAPESSIAHGIEAERELLEPIKQRAEVVMDTTEIKAADLRRRIAA
                     ELLPRAVEQRLAVTITSFGFKHGPPRDADLTLDVRFLPNPHYVSELKPLTGLDPRVVE
                     YVGRDGQLGTFYEKLEPLLDFLLPAYMAEGKAHLMVAIGCTGGRHRSVAIAEHLGALY
                     GARDDLFVEVVHRDAGKA"
     gene            complement(2127..>2802)
                     /locus_tag="VFY44_05655"
     CDS             complement(2127..>2802)
                     /locus_tag="VFY44_05655"
                     /inference="COORDINATES: protein motif:HMM:NF020048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX5909958.1"
                     /translation="PMRIECFDISTLGETHTVASMVVFEGGAPKKSDYRRFKVRGAAQ
                     DDFAAMAEVLSRRVAQYRTQQELSPHEKQYDASFGSLPTLIVIDGGKGQLGAGLKELA
                     ELTELGTTVISLAKRIEEVFIPGRPRPLVMGHDEAPLQLLQRIRDEAHRFAIEFHRGR
                     RDKAMTHSFLDDLPGVGPTRKRALLAHFGTPDRILKASREELEGVSGLPGKVAREMHQ
                     YLHRVD"
BASE COUNT          376 a         1066 c          994 g          366 t
ORIGIN      
        1 acgtccacga tcgccagatc ggggcggtgg ccgccgagga cgcgcagcag gccctcgccg
       61 tgctcggcga cggcgacgac gtcgatgtcg cgctcgcgca gcagggcgac gagcccctcg
      121 cgcagcagcg cctggtcctc ggcgatgact acgcgcacgg cagctccgcc gtgaccgtgg
      181 tgcccgcacc ggcggcgctg gagaccacga gggtgccgtc cagcgcctcg acgcgggcgc
      241 gcaggccctc gaggccgccg cccaggtcca cctgggcgcc gcccggcccc tcgtcgcgga
      301 ccgcggcggt cagccggtcg ccgagctggc tgacggtcac gtgcgcgggc gcgccgccgc
      361 cgtgcttgac cgcgttggtc agcgcctcgg ccaccacgaa gtaggcggcg gtctcgatga
      421 ccggcgcggg ccggcccgcc agctgggcgg tcaccgtgac cgggatcggc gcccgccggc
      481 ccagcgcctc gacggccgcg ggcagcccgc ggtcggccag caccggcggc gcgatgcccc
      541 gcgccaggtc gcgcagctcg ccgatcacgg cgctggcctc gtcgcgggca cggcggaact
      601 cggccgccac ctcgggctgg tcgccgagcc tctcctcggc ccgtcccagc tgcatgctca
      661 gcgccaccag ccgcgcctgc gcgccgtcgt gcaggtcacg ctcgatccgg cgcagctcgg
      721 ccgcctccac gtccagcgcg ccgcgccggg tgcgggtcag ctcctcgacg cgccggttca
      781 gcttctgctc gcgctcgggg tgcatctgga accacagcac cgcgatcagc cagtgcgcgg
      841 ccagcgccac cgagtagccc agcgcggacc agtacggcca cagcgggccg aagcccgtgc
      901 ccgcccacag gccgatctcc atcaccacga ggaccgccga gaccgcgccc tgcacggcca
      961 gccgccgggt cagccccggg gtctgcgccc acgcccagcg cagcgacacc accagcgcca
     1021 tcagcagcag cagcccgaag gccacccagc cgggccagaa cacgccgccg ccggtcaggc
     1081 cccacagcgc ggtcagcccg agcacgacca cggccgagac ggcggcctgg cgctggagca
     1141 gttcgtcgtg ggccaggacg ggcatcgaac cgaatatgcc agagccgttg ggccctgccc
     1201 agggggccag ggaccgtccg cgggtggggc taacccccgg gctcaggcct tgccggcgtc
     1261 ccggtgcacg acctcgacga agaggtcgtc gcgggcgccg tagagcgccc ccaggtgctc
     1321 ggcgatggcc acgctgcggt ggcggccgcc ggtgcagccg atggccacca tcaggtgcgc
     1381 cttgccctcg gccatgtacg ccggcagcag gaagtccagc agcggctcga gcttctcgta
     1441 gaaggtgccc agctggccgt cgcggcccac gtactcgacc acgcgcggat cgagcccggt
     1501 caggggcttg agctcggaca cgtagtgcgg gttgggcagg aagcgcacgt ccagcgtcag
     1561 gtcggcgtcg cgcggcggcc cgtgcttgaa gccgaacgag gtgatcgtga ccgccagccg
     1621 ctgctcgacg gcgcgcggca gcagctcggc cgcgatccgc cggcgcaggt ccgccgcctt
     1681 gatctcggtg gtgtccatga ccacctcggc ccgctgcttg atgggctcca gcagctcgcg
     1741 ctcggcctcg atcccgtgtg cgatcgagga ctccggcgcc agcggatggc gccggcgcgt
     1801 ctccttgtag cggtccagca gcgtgtcctc ggcggcgtcc aggaacagga tccggtgcgg
     1861 caggtcggcc gcctggaggc tgtccaccgc atccgcgagc gactcgaagt aggtgccgcc
     1921 acggacgtcg cacaccacgg ccgcgcgctc gaccttcgag ccctcgtggg cgaacaggtc
     1981 caccaggccg cggatcatct ccggcggcag gttgtccacg cagaagtagc ccgcgtcctc
     2041 gaacacgttc atggcggtcg acttgccggc gcccgagtag ccggtgatca ccacgaggtc
     2101 gcgcaggcgc gtcctgatct cggccatcag tcgaccctgt ggaggtactg gtgcatctca
     2161 cgcgccactt tgcccggtag cccggacact ccctccagct cctcgcggct ggccttgagg
     2221 atccggtcgg gggtgccgaa gtgcgccagc agcgcccgct tgcgggtggg ccccaccccg
     2281 ggcaggtcgt ccaggaacga gtgggtcatg gccttgtcgc ggcggccgcg gtggaactcg
     2341 atcgcgaacc ggtgcgcctc gtcgcggatg cgctggagca gctgcagcgg ggcctcgtcg
     2401 tggcccatca ccagcggccg cggccggccc gggatgaaca cctcctcgat ccgcttggcc
     2461 aggctgatca ccgtcgtgcc cagctcggtc agctcggcca gctccttcag gccggcgccc
     2521 agctggccct tgccgccgtc gatcacgatc agcgtgggca gcgacccgaa gctggcgtcg
     2581 tactgcttct cgtggggcga gagctcctgc tgggtgcggt actgggccac ccgccgcgac
     2641 agcacctcgg ccatggcggc gaagtcgtcc tgggcggcgc cgcggacctt gaagcggcgg
     2701 tagtcggact tcttcggcgc gcccccctcg aacaccacca tcgaggcgac cgtgtgcgtc
     2761 tcgcccaggg tggagatgtc gaagcactcg atcctcatgg gc
//
LOCUS       DASUPW010000248         3158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1442677, whole genome shotgun sequence.
ACCESSION   DASUPW010000248 DASUPW010000000
VERSION     DASUPW010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442677"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VFY44_05660"
     CDS             <1..186
                     /locus_tag="VFY44_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909959.1"
                     /translation="GFSANAAKATEEKEKEKDKDAGAKAGEAKQQDEPAAGEAKDESD
                     AGTEDEQKPAAGTVVAP"
     gene            183..1682
                     /locus_tag="VFY44_05665"
     CDS             183..1682
                     /locus_tag="VFY44_05665"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5909960.1"
                     /translation="MIKQSPSLGKMLAMGAFALSCVAILLYLWLTFGGSVPLRSEGYR
                     FTIKFPEATQLAQEADVRISGVNVGKVKTKKPDNQTGLTSAVLELQSQYAPIPRDTRA
                     ILRQKTLLGETYVELTPGNRDAPPLNDGGTLSTAQVAPTVELDEIFRAFDPKTRRAFS
                     TWLDAQGRGVEKGGADLNRALAQLTPFAEDVDDVLRILRRQDGATRGLVRDTGEVFNA
                     LSERRGQLQSLITNSNRVFETTAARDRQLADTFVAFPTFLREARTTTRRLTTFAQDTD
                     PLVTQLRPAARELSPTLQNVQAIAPDLRGLFTDLGPLIKVSRKGLPALDGILDDSRPL
                     LARFEPYLRTLQPTFDYLGLYKREITALLGNAAGTTQAVDPGTNTSKLIHYLRTTNPI
                     SPEVLATYEKRLPSNRSNPYPEPGAYTQLARGLPTFGSYLCANPGPATSLAPSSRNPL
                     LTDNVRRLVEKYAYTPRGVVSPSCREQAPLGRLVEQGGRFPQLRPIEAP"
     gene            1693..>3158
                     /locus_tag="VFY44_05670"
     CDS             1693..>3158
                     /locus_tag="VFY44_05670"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5909961.1"
                     /translation="MREDFFGSLARAVTRRPTLVLAVVGVLALAGAALALRLEPQTGV
                     DTLVSGSSDRAKASEGFKKDFGDDAVVILVKGSKGKGDLQRTLLSPDLGRLIKLEGCL
                     SGNVPEKGLKQLPAPCRELAKLKPAKVVYGPGTFINTAVNQITDQLTQRQQATTQQAE
                     QVAESARKASAKRGDSKARQRQLAAAARNQVQSQFTQQTLQLALRYGLTGLPSLDNPD
                     FISTLVFDNKRGVGQPKTRFAYLFPSSTSAVVQVRLRPDLSEADRARSIELIQEVTRD
                     KALKPTAGARYVVTGIPVVVQGLADAVRSAIFVLLGAALLLMAATLALVFRTRLRLLP
                     LAVALAAAALTFGALSLAGGSLTMASIAVLPVLIGLAVDYAIQFQARHDEGRRRGLGD
                     REAAVSAATTGGPTIATAGLATAVGFLVLLLSPVPLVRGFGALLVLGVVLALACAVTA
                     GFAALVRLAPAERGPRLPGVRARAQGAWDRVAGSGAGQA"
BASE COUNT          462 a         1197 c         1104 g          395 t
ORIGIN      
        1 ggcttcagcg ccaacgccgc caaggcgacg gaggagaagg agaaggagaa ggacaaggac
       61 gccggcgcca aggccggtga ggccaagcag caggacgagc ccgccgccgg cgaggccaag
      121 gacgagtccg acgccggcac cgaggacgag cagaagccgg cagccgggac ggtggtggcc
      181 ccgtgatcaa gcagagcccc agcctgggca agatgctggc catgggggcg ttcgcgctct
      241 cgtgcgtggc gatcctgctc tacctctggc tgaccttcgg cggctcggtg ccgctgcgct
      301 cggagggcta ccggttcacg atcaagttcc ccgaggccac ccagctggcc caggaggccg
      361 acgtccgcat ctcgggcgtg aacgtgggca aggtcaagac caagaagccc gacaaccaga
      421 ccgggctgac ctcggccgtg ctggagctgc agagccagta cgcgcccatc cccagggaca
      481 cccgcgcgat cctgcgccag aagacgctgc tgggcgagac ctacgtggag ctcaccccgg
      541 gcaaccgcga cgccccgccg ctgaacgacg gcggcacgct gagcacggcc caggtggcgc
      601 ccacggtgga gctggacgag atcttccgcg ccttcgaccc caagacccgg cgcgcgttca
      661 gcacgtggct ggacgcccag ggccgcggcg tcgagaaggg cggcgcggat ctgaaccggg
      721 cgctggccca gctgaccccc ttcgccgagg acgtggacga cgtgctgcgc atcctgcgcc
      781 gccaggacgg cgccacgcga ggcctggtgc gcgacacggg cgaggtcttc aacgcgctga
      841 gcgagcgccg cggccagctc cagagcctga tcaccaactc gaaccgggtc ttcgagacca
      901 ccgccgcgcg cgaccgccag ctggccgaca ccttcgtggc cttcccgacc ttcctgcgcg
      961 aggcgcgcac caccacccgg cgcctgacca cgttcgccca ggacacggac ccgctggtga
     1021 cccagctgcg tcccgcggcc cgcgagctct cgccgacgct ccagaacgtg caggccatcg
     1081 cgccggacct gcgcggcctg ttcaccgacc tcggcccgct gatcaaggtc tcgcgcaagg
     1141 gcctgccggc gctggacggc atcctggacg acagccgccc gctgctggcc cggttcgagc
     1201 cctacctgcg cacgctgcag cccacgttcg actacctggg cctgtacaag cgcgagatca
     1261 ccgcgctgct gggcaacgcc gccggcacca cgcaggcggt ggacccgggg accaacacca
     1321 gcaagctgat ccactacctg cgcaccacca acccgatcag ccccgaggtc ctggccacct
     1381 acgagaagcg cctgcccagc aaccgctcga acccctaccc cgagccgggg gcctacaccc
     1441 agctggcgcg cgggctgccc acgttcggca gctacctgtg tgccaacccc ggcccggcga
     1501 cctcgctggc gccctcctca cgcaacccgc tgctgaccga caacgtccgg cgcctggtcg
     1561 agaagtacgc ctacaccccg cgcggcgtgg tctcgccgtc ctgccgcgag caggccccgc
     1621 tcggccggct cgtggagcag gggggcaggt tccctcagct gcgtccgatc gaagctccct
     1681 agtctccggc tcgtgaggga ggacttcttt ggctcgctgg cgcgggcggt cacgcggcga
     1741 cccacgctgg tgctggccgt ggtgggcgtc ctggcgctgg ccggggcggc gctcgccctg
     1801 cgcctcgagc cccagaccgg cgtggacacg ctcgtgtccg gctcgtccga ccgggccaag
     1861 gccagcgagg gcttcaagaa ggacttcggc gacgacgccg tggtgatcct ggtcaagggc
     1921 agcaagggca agggcgacct ccagcgcacg ctgctcagcc cagacctggg ccggctgatc
     1981 aagctcgagg gctgcctgtc gggcaacgtc ccggagaagg gcctcaagca gctgccggcg
     2041 ccgtgccgcg agctggccaa gctcaagccg gcaaaggtgg tctacgggcc cggcaccttc
     2101 atcaacaccg ccgtcaacca gatcaccgac cagctgaccc agcgccagca ggccacgacc
     2161 cagcaggccg agcaggtggc cgagtcggcc cgcaaggcct cggccaagcg cggggactcg
     2221 aaggcgcgcc agcgccagct ggccgcggcc gcccgcaacc aggtccagag ccagttcacc
     2281 cagcagaccc tgcagcttgc gctgcgctac ggcctgacgg gcctgccctc gctggacaac
     2341 cccgacttca tctccacgct ggtgttcgac aacaagcgcg gggtgggcca gcccaagacg
     2401 cgcttcgcgt acctgttccc cagctccacc tcggcggtgg tccaggtgcg gctgcggccg
     2461 gacctgagcg aggccgaccg ggcacgctcg atcgagctga tccaggaggt cacgcgcgac
     2521 aaggccctga agcccaccgc cggtgcgcgc tacgtggtca ccggcatccc ggtggtcgtc
     2581 caggggctgg ccgacgccgt gcgcagcgcc atcttcgtgc tgctgggcgc ggcgctgctg
     2641 ctcatggcgg cgacgctggc gctggtgttc cgcacccggc tgcgcctgct gccgctggcg
     2701 gtggcgctgg ccgccgccgc gctgaccttc ggcgccctgt ccctggccgg cggcagcctg
     2761 accatggcct cgatagccgt cctgcccgtg ctgatcggcc tggcggtgga ctacgcgatc
     2821 cagttccagg cccggcacga cgagggccgc agacgcgggc tcggcgaccg cgaggccgcg
     2881 gtgtcggcgg ccacgaccgg cgggcccacc atcgccacgg ccggcctggc cacggccgtc
     2941 ggcttcctgg tgctgctgct gtcgccggtg ccgctggtgc gcgggttcgg cgcgctcctg
     3001 gtgctgggcg tggtgctggc gctggcctgc gccgtgaccg ccggcttcgc cgcgctggtg
     3061 cgcctggcgc ccgccgagcg gggcccgcgg ttgcccggcg tgcgcgcccg cgcgcagggc
     3121 gcctgggacc gcgtggccgg ctccggagcg gggcaggc
//
LOCUS       DASUPW010000249         3189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1996997, whole genome shotgun sequence.
ACCESSION   DASUPW010000249 DASUPW010000000
VERSION     DASUPW010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1996997"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VFY44_05675"
     CDS             <1..431
                     /locus_tag="VFY44_05675"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX5909962.1"
                     /translation="RWRRRRDLDRGEARCTRQRRHRAFDLGPGPVQLAPGEELARADP
                     AAPGETVHGVARRRGVSTSQLFTWRKQLRAEAGLPPSPLTVPGFAAVAIAPVPPLAGD
                     AAEAPSGLIEIELADGGRVRISGAADPAVVAAALRALAGR"
     gene            428..775
                     /gene="tnpB"
                     /locus_tag="VFY44_05680"
     CDS             428..775
                     /gene="tnpB"
                     /locus_tag="VFY44_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536669.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEX5909963.1"
                     /translation="MIPFPGGARVWLATGRTDMRKGMNGLALQVQEVLERDPYSGHLF
                     VFRGRRGDLLKILWHDDQGMCLFVKRLEAGRFVWPSPAAGVVTITPGQLGYLLEGIDW
                     RMPRHTWRPVAAG"
     gene            933..2504
                     /locus_tag="VFY44_05685"
     CDS             933..2504
                     /locus_tag="VFY44_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEX5909964.1"
                     /translation="MLHAERAARQTAEARLAERDVAIARQADEVAALRLEIERLKLLL
                     AKARRAQFGRSSERGAELVEQLELRLADLEESVAEEEASAEIAAPGKRASPSGGAARK
                     PARRPLPAHLPRERIVYPAPCSCPECGGPVRKLGEDVTETLECEPRRWKVVEHVREKV
                     SCRACEAVSQPPAPSHPIARGRAGPGLLALVLAAKYGQHLPLTRQSAIYAREGVEIDV
                     STLADWVGSSTTALVPMVLAIRTHVFAALRLHGDDTTVPVLAKGRTRTGYVWAYVRDD
                     RPFGGPDPPAVAFLYSPDRRGEHPERHLAGFAGILQADAYAGFDRLYAPKRAPGPILE
                     AACWAHARRKLFELAAVGKAPVAAEAVRRIDELFAIERDISGKPAAERLAAREERAKP
                     LVLELEAWLRVQHARVSPKSETGKAIAYALNHWKALTRFLGDGRICLSNNAAERALRG
                     VAVGRRNWTFAGSDRGGERAAAIYTLIETCKLNGVDPQAWLADVLARLPDHPAKRIGD
                     LLPWNWAAARNISPS"
     gene            2545..3063
                     /locus_tag="VFY44_05690"
     CDS             2545..3063
                     /locus_tag="VFY44_05690"
                     /inference="COORDINATES: protein motif:HMM:NF040080.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6398 domain-containing protein"
                     /protein_id="HEX5909965.1"
                     /translation="MASFDRPTSVPKGMQATYDAVVHLTDEVCDRHLNAEYRELARAM
                     AAALCRKRPSPLASGQPRTWACAILYELGRVNFLSDRASQPYMTLAELCTAFGVSQGT
                     ASAKARVIENTLRVRPFDPEWTLPSLRDQNPLTWMAEVNGLLVDLRQMPREVQEIAFE
                     KGMIPYIPADRK"
BASE COUNT          431 a         1164 c         1174 g          420 t
ORIGIN      
        1 gacggtggcg tcgcaggcgc gatctcgacc gcggcgaagc ccggtgcacc cggcagcgcc
       61 ggcaccgtgc gttcgatctc gggcccggcc cggtacagct tgcgccaggc gaagagctgg
      121 ctcgggctga ccccgctgcg ccgggcgaga cggtgcacgg ggtggcgcgg cggcgcgggg
      181 tcagcaccag ccagctcttc acctggcgca agcagctgcg cgccgaggcc gggctcccgc
      241 cgtccccgct gacggtgccg ggcttcgcgg ccgtggcgat cgcgcccgtg ccgccgctcg
      301 cgggcgatgc ggcggaggcg ccatccggcc tgatcgagat cgagctggcg gacggtggcc
      361 gggtgcggat ctcgggtgct gccgacccag cggtggtcgc ggcggcgctc cgcgcgctgg
      421 ccgggcgatg atcccgttcc cgggcggggc gcgggtctgg ctggccaccg gccgcaccga
      481 catgcgcaag ggcatgaacg gtctcgcgct ccaggtgcaa gaggtgctcg agcgtgatcc
      541 gtactcgggc catcttttcg tctttcgtgg ccgccggggt gatcttttga agatcctctg
      601 gcatgacgac cagggcatgt gcctgttcgt gaagaggctg gaggccggcc ggttcgtctg
      661 gccctcgccg gccgcgggcg tcgtgacgat tacaccaggt cagctcggct atttgctcga
      721 aggaatcgac tggaggatgc cccgacacac ctggagaccc gtcgcggcgg gctgatcggg
      781 cctgtcggag gctcccgatc gcgccggggc cgggctgcgg cacggtgact cgggtggggc
      841 gatccgggcc gacaagccgg cggctatgtg cttgaatctc cgtcgtgaac agccccgagc
      901 cgctccccga cgatctcgcc gcggcgcacg cgatgctgca cgccgagcgc gcggcacggc
      961 agaccgccga ggcgcggctt gccgagcggg acgtcgcgat cgcccgccag gccgacgagg
     1021 tcgccgccct ccggctcgag atcgagcgcc tcaagctcct gctcgccaag gcgcgccggg
     1081 cgcagttcgg ccgctcgtcc gagcgcgggg ccgagctcgt cgagcagctc gagctgcggc
     1141 tcgccgatct cgaggagagc gtggccgaag aggaggcctc ggccgagatc gcggcacccg
     1201 ggaagcgggc ctcgccgtca ggcggcgccg cccgcaagcc ggcccgtcgg ccgctgccgg
     1261 cgcacttgcc gcgcgagcgc atcgtctacc cggcgccctg cagctgcccc gagtgcggcg
     1321 gcccggtgcg caagctgggc gaggacgtga ccgagacctt ggagtgcgag ccgcgccgct
     1381 ggaaggtggt cgagcacgtg cgcgagaagg tcagctgccg cgcctgcgag gcggtcagcc
     1441 agcctccggc gccctcgcac ccgatcgccc gcggccgggc cggccccggc ctgctggcgc
     1501 tggtgctggc ggccaagtac ggccagcacc tgccgctcac gcggcagagc gcgatctacg
     1561 cccgggaagg ggtggagatc gacgtcagca ccctcgccga ctgggtgggt tcgtcgacca
     1621 ccgccctggt gccgatggtg ctggcgatcc ggacccacgt cttcgcggcc ctgcggctgc
     1681 acggcgacga caccacggtc ccggtgctgg ccaaggggcg gaccaggacc gggtacgtgt
     1741 gggcgtacgt gcgcgacgac cggccgttcg gcgggcccga cccgccggcg gtggcgttcc
     1801 tctactcccc ggaccgccgc ggcgagcacc ccgagcgcca cctggccggg ttcgccggca
     1861 tcctgcaggc ggacgcctac gccggctttg accggctcta cgccccgaag cgcgcgccgg
     1921 ggccgatcct cgaggccgcc tgctgggcgc acgcccgcag gaagctgttc gagctggccg
     1981 cggtcggcaa ggcgccggtc gcggccgagg cggtgcggcg gatcgacgag ctgttcgcga
     2041 tcgagcgcga catcagcggc aagcccgccg cggagcgcct ggcggcccgg gaggaacggg
     2101 cgaagccgct ggtgctcgag ctcgaggcct ggctgcgcgt ccagcacgcg cgggtttcgc
     2161 ccaagtccga gaccggcaag gcgatcgcct acgcgctcaa ccactggaag gcgctcaccc
     2221 gcttcctcgg ggacgggcgc atctgcctga gcaacaacgc ggccgagcgc gcgttgcgcg
     2281 gcgtggcggt cgggcgccgg aactggacct tcgccggctc cgaccggggc ggcgagcggg
     2341 cggcggcgat ctacaccctg atcgagacct gcaagctcaa cggcgtcgac ccacaagcct
     2401 ggctggccga cgtactggcc cgactgccgg atcatcccgc caagcggatc ggcgacctcc
     2461 tgccatggaa ctgggcggcc gcgcgaaaca tctccccgag ctgagcccgc gcgccaccgg
     2521 gctccacccg gcacgaggtc cggcatggcg agcttcgacc gcccgacgtc cgtccccaag
     2581 ggcatgcagg cgacctacga cgcggtcgtg cacctgacgg acgaggtctg cgaccggcac
     2641 ctgaatgccg agtaccgcga gctggcgcgg gccatggcgg ccgccttgtg ccgcaagcgg
     2701 ccgagcccgc tcgcctcggg ccagccgcgg acctgggcct gcgccatcct ctacgagctg
     2761 ggccgggtca acttcctctc cgatcgcgcg tcgcagccct acatgaccct ggccgagctg
     2821 tgcaccgcgt tcggggtcag ccagggcacc gcgagcgcca aggcgcgcgt catcgagaac
     2881 acgctccgcg tccgcccgtt cgacccggag tggacgctgc cgagcttgcg cgaccagaac
     2941 ccgctaacgt ggatggccga ggtcaacggc ctgctggtcg acctgcgcca gatgccgcgc
     3001 gaggtccagg agatcgcgtt cgagaagggg atgatcccct acatccccgc cgaccgaaag
     3061 tgaggttcgg caccacgccg tgccggcgca cctcgatcac ggccgtcaac ccccgacgcc
     3121 gcgtccctca ccggaggcat acttgaggtt ccttaccgac gccgctgcta ttgggcgctt
     3181 cggatcgaa
//
LOCUS       DASUPW010000250         3332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_196365, whole genome shotgun sequence.
ACCESSION   DASUPW010000250 DASUPW010000000
VERSION     DASUPW010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196365"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VFY44_05695"
     CDS             complement(<1..584)
                     /locus_tag="VFY44_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5909966.1"
                     /translation="MALSTQRLTLAYGDRPVVQDLDLEIMEGGLTAIVGANACGKSTL
                     LRGLARLLKPRAGAIMLDGRSIAEIPTRELARRLGLLPQTPVAPEGVTVEDLVSRGRY
                     PHQTFLQQWSEEDQEKVDWAIEATAIGDLRTRVLDELSGGQRQRAWVAMALAQDTELL
                     LLDEPTTYLDLAHQIEVLDLLQELNERDGRTIVMV"
     gene            complement(587..1627)
                     /locus_tag="VFY44_05700"
     CDS             complement(587..1627)
                     /locus_tag="VFY44_05700"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX5909967.1"
                     /translation="MSSVTVRSRRVGVSARIDARLAAVTAALAVACLLAVAGNVSYGE
                     FGLPVTDVVATLFGFGDQATRVIVLDLRLPRALVALLAGAALAMSGVIFQTFGRNALA
                     SPDIIGVTTGAAFAGVSAFVLGASPELAPLAAFGGALTATALMYALAWRGGLSPFRLV
                     LVGVGISALALAGISFVLIQGRVEQIQEAYTWLVGSVNLRAWSDFWPLAAGLAVLVPA
                     AAVLTRSLDALALGDETARSLGVHVERTRLALVTVGAGLAAVGVAAAGPVAFVAFVAP
                     LIARRLTGVHGAAVMPAAALCGAAMMPAADVLGRVVFAPTEMPVGIVTSLLGAPVFLF
                     LLFRANRARGAA"
     gene            complement(1624..2628)
                     /locus_tag="VFY44_05705"
     CDS             complement(1624..2628)
                     /locus_tag="VFY44_05705"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX5909968.1"
                     /translation="MTGALKPTAGLAALLTGVVVAGLASLALGSLSVSLHDVVGAFTG
                     FDDSDAQVIVRDVRGPRTLVALAVGAGLGTAGAVAQGVTRNPLADPGILGISLGASFF
                     VVAAIFVLGISSALGYVWFAFIGAVVAGVTVYGLGATGPGNRAAPINLALAGAAVAAL
                     FGALTSAVLVVDTQTLNEFRFWVVGSVAGRDLDTLAVAGPFMAVGLAGALFAGRALNA
                     LALGEDVARALGQRVVRTRAVLLASFVLLSGGAVAIAGPIAFVGLIVPHIARGLVGAD
                     YRWVVPYSALIGAILITGADVVGRLVAKPDEIQVGIVMAVLGVPVFITLVRRRKLAEV
                     "
     gene            complement(2664..>3332)
                     /locus_tag="VFY44_05710"
     CDS             complement(2664..>3332)
                     /locus_tag="VFY44_05710"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX5909969.1"
                     /translation="VIFWLQEAGLPMTGANVARAMQVSAPTVHEMIGRLEKDGYVTRA
                     GDKSLSFTETGLEHAEGIVRRHRLIERFLTDVFDIPWDQVHEEAEKLEHWMSPTVEER
                     MLRAIGDVKTCPHGHPIFTKERIEGVPLADVEEGAKVTVLRFENEAEDLLHYLMDAGL
                     TPGQEGTLSQSGDEEVAFEVEGKRLAVTRSVAETVSVLADPSPPQRTALPEHLVLAKE
                     HWGR"
BASE COUNT          515 a         1266 c         1183 g          368 t
ORIGIN      
        1 accatcacga tcgtccgccc gtcgcgctcg ttgagctcct ggagcagatc cagcacctcg
       61 atctggtggg ccaggtccag gtacgtggtg ggctcgtcga gcagcaggag ctcggtgtcc
      121 tgcgccagcg ccatggccac ccaggcccgc tggcgctgac cgcccgagag ctcgtccagc
      181 acgcgcgtgc gcaggtcgcc gatggctgtg gcctcgatgg cccagtccac cttctcctgg
      241 tcctcctcgg accactgctg gaggaaggtc tggtgcgggt agcgcccgcg cgagaccagg
      301 tcctcgaccg tcacgccctc gggggcgacc ggtgtctggg gcagcagccc caggcggcga
      361 gccagctcgc gcgtggggat ctccgcgatc gaccggccgt ccagcatgat cgcgccggcg
      421 cgcggcttga gcaggcgggc cagcccgcgc agcagcgtgg acttgccgca cgcgttggcg
      481 ccgacgatgg ccgtcagccc gccctccatg atctccaggt ccaggtcctg gaccacgggc
      541 cggtcgccgt aggccagcgt cagcctctgc gtgctcaggg ccacggtcag gccgctcccc
      601 gggcgcggtt ggcacggaac agcaggaaca ggaacaccgg cgctcccagc agcgaggtca
      661 cgatccccac cggcatctcg gtgggcgcga acacgacccg gcccagcacg tcggccgcgg
      721 gcatcatcgc ggcgccgcac agcgcggcgg ccggcatcac cgcggcgccg tgcacgccgg
      781 tcagccggcg cgcgatcagc ggggccacga aggccacgaa cgccaccggc ccggcggcgg
      841 cgacgccgac cgcggccagc cccgcgccca ccgtgaccag cgccaaccgg gtccgctcga
      901 cgtgaacgcc caggctccgg gccgtctcgt cgcccagggc cagcgcgtcc agggaccggg
      961 tcagcacggc cgcggccggc accaggacgg ccagccccgc ggccagcggc cagaagtcgc
     1021 tccacgcgcg caggttcacg ctgcccacca gccacgtgta ggcctcctgg atctgctcca
     1081 cccggccctg gatgagcacg aaggagatgc cggccagcgc cagcgccgag atgccgacgc
     1141 cgaccagcac gagccggaac ggcgacaacc cgccgcgcca ggccagcgcg tacatcagcg
     1201 cggtcgcagt cagcgcgccg ccgaacgcgg ccagcggcgc cagctcggga gaggcgccca
     1261 gcacgaacgc ggacacgccg gcgaacgccg caccggtcgt cacgccgatg atgtccggcg
     1321 acgccagcgc gttgcggccg aaggtctgga agatcacgcc cgacatggcc agcgcggccc
     1381 cggccagcag cgcgaccagc gcgcgcggca ggcggaggtc cagcacgatc acgcgcgtgg
     1441 cctggtcgcc gaagccgaag agcgtggcca ccacgtcggt cacgggcagg ccgaactcgc
     1501 cgtaggacac gttgccggcc accgccagca ggcacgccac ggccagggcc gcggtgacgg
     1561 cggccaggcg cgcgtcgatg cgcgcggaca cgccgaccct gcgcgagcgg acggtcaccg
     1621 agctcatacc tcggccagct tccggcgccg caccagcgtg atgaacacgg gcacgccgag
     1681 gacggccatc acgatgccca cctggatctc gtcgggcttg gccaccaggc ggccgaccac
     1741 gtcggcgccc gtgatcagga tcgccccgat cagcgccgag tacggcacca cccagcggta
     1801 gtccgcgccc accagcccgc gggcgatgtg ggggacgatc aggcccacga acgcgatcgg
     1861 gccggcgatg gccaccgcgc ccccggacag cagcacgaac gaggccagca gcacggcgcg
     1921 cgtgcggacc acgcgctggc ccagcgcccg cgcgacgtcc tcacccagcg ccagcgcgtt
     1981 cagcgcgcgg ccggcgaaca gggccccggc caggccgacg gccatgaacg gcccggccac
     2041 ggcgagcgtg tccaggtcgc ggcccgccac cgagcccacg acccagaagc ggaactcgtt
     2101 cagcgtctgg gtgtcgacca cgagcacggc cgacgtgagc gcgccgaaca gggccgcgac
     2161 cgcggccccc gccagcgcca ggttgatcgg ggccgcccgg ttgcccggcc cggtcgcgcc
     2221 gagcccgtac accgtgacgc ccgccacgac ggcgccgatg aaggcgaacc agacgtagcc
     2281 cagcgccgag ctgatgccca gcacgaagat ggccgcgacc acgaagaacg aggcacccag
     2341 ggagatcccg aggatgccgg ggtcggccag cgggttgcgg gtcacgccct gggcgaccgc
     2401 ccccgccgtc ccgaggccgg cgcccacggc cagggccacc agggtgcgcg ggccccgcac
     2461 gtcgcggacg atgacctgcg cgtcggagtc gtcgaagccg gtgaaggcgc cgacaacgtc
     2521 gtgcagcgag accgaaagcg agcccagcgc cagcgatgcc agcccggcca cgacgacccc
     2581 ggtcaggagc gccgccaggc ccgcggtcgg tttgagcgcg ccggtcacgc gccccgatgt
     2641 tagttagggc agcctaataa ggctcagcgg ccccagtgct ctttggcgag cacgaggtgt
     2701 tccggcagcg cggtgcgctg cggcggcgag gggtccgcga gcacggagac ggtctcggcg
     2761 acgctgcggg tgacggccag gcgcttgccc tcgacctcga aggccacctc ctcgtcgccg
     2821 ctctggctga gcgtgccctc ctggcccggc gtcaggccgg cgtccatcag gtagtgcaga
     2881 aggtcctcgg cctcgttctc gaagcgcagg acggtgacct tggcgccctc ctccacgtcg
     2941 gccagcggca cgccctcgat gcgctccttg gtgaagatcg ggtggccgtg gggacaggtc
     3001 ttgacgtcgc cgatggccct gagcatccgc tcctcgacgg tcggcgacat ccagtgctcg
     3061 agcttctcgg cctcctcgtg cacctggtcc cacggaatgt cgaacacgtc ggtcaggaag
     3121 cgctcgatca ggcggtggcg gcggacgatg ccctcggcgt gctcgaggcc cgtctcggtg
     3181 aaggagagcg acttgtcgcc ggcgcgggtc acgtagccgt ccttctccag ccggccgatc
     3241 atctcgtgca ccgtcggggc ggacacctgc atggcccggg cgacgttggc gcccgtcatg
     3301 ggcaggccgg cctcctggag ccagaagatg ac
//
LOCUS       DASUPW010000251         2464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1997206, whole genome shotgun sequence.
ACCESSION   DASUPW010000251 DASUPW010000000
VERSION     DASUPW010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1997206"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFY44_05715"
     CDS             <1..207
                     /locus_tag="VFY44_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909970.1"
                     /translation="DSVHCELKAGAQVIDQVDMKTLPALAAIPVSMQAVTTTPVVDQV
                     SITCDVSVANGSANYSSIIAVPAN"
     gene            complement(238..1686)
                     /locus_tag="VFY44_05720"
     CDS             complement(238..1686)
                     /locus_tag="VFY44_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909971.1"
                     /translation="MSFTARLALLGAVLLTSLLAVPPAFAAGQPGVQRLSYRAGPYHV
                     GPGQNKIEFAPTAQKPQVDGYIVGIRPNLRHADGSIPKTNRIMFHHGVWVNLKGRDAT
                     SGGPERFYAAGEEKTNLALPKGYGYAYKASDPWILNYMIHNLVARADDVYITYDIDFI
                     PASSPAAAAIKPARPVWMDVENGKLYPVFDVFKGSGRKGMFTYPTQNPSAYAGRSSRP
                     NRWRVDRDSVLISTAGHLHAGGLWNDLYVNRGGRRARLFRSVAKYFEPLGPVSWDLAM
                     TVTPEKYRVGLKKGDLLSTTVTYESKRASWYESMGIMVSWLADAGPGVDPFKRKVDVE
                     GKTTHGHLPENDVHGGKDIDAENPSDVASGAFSSNIAISNFGYLPKNRMGGRTPVVRR
                     GQSITFTSTDAAQDIYHSVTSCRLPCNKSTGIGYPIANGPVEFDSGQLGFGGPPTPDR
                     NTWSTPKRLKTGTYAYFCRIHPAMRGAFRVKK"
     gene            1785..2204
                     /locus_tag="VFY44_05725"
     CDS             1785..2204
                     /locus_tag="VFY44_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909972.1"
                     /translation="MISANRRLIALSAVAVALVCAPFALAASSGTYKGKIEYQGYDVK
                     FKVSGGKVTKFSARMLQDCGDGLASFTIAPKGGFPIKGNKVDAKRTDTYGKSKATVEL
                     KGKFSGSKFTGSVREYDFVPGSGIVCDTLKRKFKAKK"
     gene            complement(2215..>2464)
                     /locus_tag="VFY44_05730"
     CDS             complement(2215..>2464)
                     /locus_tag="VFY44_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909973.1"
                     /translation="CSRDCSLQVTVELSGTAGATRAPKARTTLRAGAGPGRGFVVRLP
                     KAAQRRLSRSRSPGLTVRLLARSGSESRALTRQVRLRR"
BASE COUNT          366 a          832 c          876 g          390 t
ORIGIN      
        1 gactcggtcc actgcgagct gaaggcagga gcccaggtga tcgaccaggt ggacatgaag
       61 acgctgcccg cgctggcggc catcccggtg tccatgcagg cggtcaccac gacgccggtc
      121 gtcgatcagg tgagcatcac ctgtgacgtc tcggtggcca acggctcggc caactacagc
      181 agcatcatcg ccgtgcccgc caactaggcc ggctccagcg cgcggcgggc cgggtcgcta
      241 cttcttgacc cggaacgccc cgcgcatggc cgggtggata cggcagaagt aggcgtaggt
      301 gccggtcttg agccgcttgg gcgtggacca ggtgttgcgg tccggcgtgg gcggcccgcc
      361 gaagcccagc tggcccgagt cgaactcgac cggtccgttg gcgatcgggt agccgatgcc
      421 cgtcgacttg ttgcacggca gccggcacga ggtgaccgag tggtagatgt cctgggccgc
      481 gtcggtgctg gtgaaggtga tcgactggcc gcggcggacc accggcgtgc gcccgcccat
      541 cctgttcttg ggcaggtagc cgaagttgct gatggcgatg ttcgagctga acgcgcccga
      601 ggccacgtcg gaggggttct cggcatcgat gtccttgcca ccgtgcacgt cgttctcggg
      661 caggtgaccg tgggtggtct tgccctccac gtcgaccttc cgcttgaacg gatccacgcc
      721 cgggccggcg tcggccagcc aggagaccat gatccccatc gactcgtacc aggacgcccg
      781 cttggactcg tacgtcaccg tcgtggacag caggtcgccc ttcttcaggc ccacccggta
      841 cttctccggg gtgaccgtca tcgccaggtc ccacgacacg gggccgagcg gctcgaagta
      901 cttggccacc gagcggaaca ggcgggcgcg cctgcccccg cggttcacgt acaggtcgtt
      961 ccacaggccg cccgcgtgca ggtgcccggc ggtgctgatg agcacgctgt cgcggtccac
     1021 ccgccagcgg ttggggcggc tcgaccggcc ggcgtaggcc gaggggttct gcgtcgggta
     1081 ggtgaacatg cccttgcgcc cggagccctt gaacacgtcg aagaccgggt agagcttgcc
     1141 gttctccacg tccatccaga ccgggcgggc gggcttgatg gccgccgcgg ccggagagct
     1201 ggcggggatg aagtcgatgt cgtaggtgat gtagacgtcg tcggcccggg ccacgaggtt
     1261 gtggatcatg tagttcagga tccacggatc ggatgccttg taggcgtagc cgtagccctt
     1321 cgggagggcc aggttggtct tctcctcgcc ggccgcgtag aagcgctcgg ggcctccgct
     1381 ggtggcgtcc ctgcccttca ggttcaccca cacgccgtgg tggaacatga tccggttggt
     1441 cttcgggatc gagccgtcgg cgtggcgcag gttcggccgg atgcccacga tgtagccgtc
     1501 gacctgcggc ttctgggccg tcggtgcgaa ctcgatcttg ttctggccgg ggccgacgtg
     1561 gtaggggccg gcgcgatagc tcaggcgctg cactccgggc tgtccggccg cgaacgcggg
     1621 cggcacggcc aggagtgagg tcaggagcac cgcgccgagc agggccaggc gagcggtgaa
     1681 ggacacgcag gcgagcctcc cacaccttcg ccgccggcgc cagcgatcgg acggatatcg
     1741 atcgcggatg gcgccgtcgt cacatccgtg tcaggttggc gcgcgtgatc tcagccaacc
     1801 gccgtctcat cgccctgtcc gccgtcgccg tcgcgctggt gtgcgccccg ttcgcgctcg
     1861 ccgcgtcctc gggcacctac aaggggaaga tcgagtacca gggctacgac gtgaagttca
     1921 aggtctccgg cgggaaggtc accaagttct cggcccggat gctccaggac tgcggcgacg
     1981 gcctggccag cttcaccatc gcccccaagg gcggcttccc catcaagggc aacaaggtgg
     2041 acgccaagcg caccgacacc tacggcaagt ccaaggccac cgtcgagctg aagggcaagt
     2101 tcagcggctc caagttcacc ggctcggtcc gcgagtacga cttcgtgccc ggcagcggca
     2161 tcgtctgcga cacgctgaag cgcaagttca aggccaagaa gtaagcgcgg ccgcctaccg
     2221 gcgcagccgc acctggcggg tcagcgcgcg cgactccgag ccgctccggg ccagcagccg
     2281 caccgtcagg ccgggcgagc gggaccggct gagcctgcgc tgtgccgcct tggggagccg
     2341 cacgacgaag ccgcgcccgg ggccggcgcc ggcgcgcagg gtcgtccgcg ccttcggcgc
     2401 ccgcgtcgcg ccggcggtcc cgctcagctc taccgtgacc tgcagggagc agtcgcgtga
     2461 gcac
//
LOCUS       DASUPW010000252         2438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_611592, whole genome shotgun sequence.
ACCESSION   DASUPW010000252 DASUPW010000000
VERSION     DASUPW010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611592"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="VFY44_05735"
     CDS             <1..872
                     /locus_tag="VFY44_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5909974.1"
                     /translation="SGDWLLPPAVLKSAYQNTPDQLELVESGGGRWAAMNTKAEPFDD
                     PNVRKAVVAAFDRQGALLALGGKAVGEVATHFLPPGTPGFDQAGGTAGTGVDFLASPT
                     GDAAVSARYMQAAGYASGKYEGDEILMVGPRDGNGKAISQLAKGAFEKVGFKVNLRLL
                     SQQVVMTKYCGNPGAAVAVCPNIGWIRDFADGQTYLDSTFNGKNILPVGNANVSQLDD
                     PGVNGLIEAAKVETDPAARASKWGEVDRAITALAPAVPLTWDRTPMAHSKNVEAVANE
                     SLGVWDFSFTALR"
     gene            952..>2438
                     /locus_tag="VFY44_05740"
     CDS             952..>2438
                     /locus_tag="VFY44_05740"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEX5909975.1"
                     /translation="MSLTWLDSPPPAPQLGFDLGEADGRVPRVPVLASVDVDGALAEG
                     FGFAAFRPGQREAVLAARDGRDVLVVMPTGSGKSLCYQLPALMRTDLTLVVSPLVSLM
                     QDQVEALSLVAPGRVAQISGLQDTATNRRALDGAISGRVRVLYVSPERFSSPGFLERL
                     REARIGLFVVDEAHCVSQWGHDFRPDYFRLADAARWLGARAIVASTATATPQVATDIV
                     ERLGLRDPVRVSTGFDRPNLAYSVVPCPSKEAVAGRIVAALSEDGALPAIVYAGTRAE
                     AERLCERLGRELSVEVVAYHAGLPRHVRADVQRRFMDGSAPVVVATNAFGMGVDKADV
                     RTVCHESVPSSIEAYYQEAGRAGRDGKPARALLFSTSRDKGLHVFFIERAAVEDDRLR
                     DVARAIARSATGSPPVYRLHINDLATDESEVESVRAIVGYMVRAGVVQPAPSGPDHVA
                     GRLVGTWDRGSLAACRVAAQEGTRQRWRQYRAVWAWVEGEGCRRAG"
BASE COUNT          316 a          859 c          913 g          350 t
ORIGIN      
        1 tctcaggcga ctggctgctt ccgccggccg tgctcaagag cgcctaccag aacacgccgg
       61 accagctcga gctcgtcgag tccggcggcg gtcgctgggc ggcgatgaac accaaggccg
      121 agccgttcga cgaccccaac gtgcgcaagg ccgtcgtggc cgcgttcgat cgccagggcg
      181 cgctcctggc gctcggcggc aaggccgtcg gcgaggtcgc gacgcacttc ctgccgccgg
      241 gcacgcccgg cttcgaccag gccggtggca cggccgggac cggcgtggac ttcctcgcct
      301 ccccgacggg tgacgccgcg gtgtcggcgc gctacatgca ggccgcgggc tacgcctcgg
      361 gcaagtacga gggcgacgag atcctcatgg tcgggccgcg cgacggcaac ggcaaggcga
      421 tctcccagct ggccaagggc gcgttcgaga aggtcggctt caaggtcaac ctgcgcctgc
      481 tctcgcagca ggtcgtcatg accaagtact gcggcaaccc gggcgcggcc gtggccgtgt
      541 gcccgaacat cggctggatc cgggacttcg ccgacgggca gacctacctg gactcgacgt
      601 tcaacggcaa gaacatcctg ccggtcggca acgcgaacgt ctcccagctc gacgatccgg
      661 gcgtcaacgg cctgatcgag gccgccaagg tcgagaccga cccggcggcg cgtgcttcca
      721 agtggggcga ggtcgaccgc gcgatcaccg cgctggcccc ggccgtgccg ctgacgtggg
      781 accgcacccc gatggcgcac tccaagaacg tcgaggcggt cgccaacgag tcgctgggcg
      841 tgtgggactt ctccttcacc gcgcttcgtt gaaagtcacc cgcagttcgt gacaaaactg
      901 cagaaaatgg agggcagcgt ccggaccggc ggctagtgtc gccggtccga catgtccctc
      961 acctggctcg acagcccgcc tcccgcgccc cagctggggt tcgacctggg tgaggcggac
     1021 gggcgggtgc cgcgggtccc cgtgctggcg tctgtcgatg tggacggcgc gttggctgag
     1081 gggttcggct tcgccgcgtt ccggcccggg cagcgcgagg cggtgctcgc cgcgcgggac
     1141 gggcgggacg tgctggtggt gatgcccacc ggctcgggca agtcgctctg ctaccagctg
     1201 ccggcgttga tgcggacgga cctgacgctg gtcgtctcgc cgctggtctc gctgatgcag
     1261 gaccaggtgg aggcgctgtc gctcgtggcg cccgggcggg tcgcgcagat cagcggcctg
     1321 caggacacgg cgaccaaccg ccgggcgctg gacggggcga tctcggggcg cgtccgtgtc
     1381 ctgtacgtct cgccggagcg cttctcctca cccggtttcc tggagcggct gcgggaggcg
     1441 cggatcggcc tgttcgtggt cgacgaggcg cactgcgtgt cccagtgggg gcacgacttc
     1501 cggccggact acttccgcct ggcggacgcg gcgcgctggc tcggggcgcg ggccatcgtg
     1561 gcctccaccg cgacggccac tccacaggtg gcgacggaca tcgtcgagcg gctcggcctg
     1621 cgcgaccccg tgcgcgtgtc caccggcttc gaccgcccga acctggccta ctcggtcgtg
     1681 ccgtgcccgt cgaaggaggc ggtggcgggg cggatcgtcg cagcgttgtc ggaggatggt
     1741 gctctgcccg ccatcgtcta cgcgggcacg cgggcggagg ccgagcggct gtgcgagcgg
     1801 ctgggacggg agctgagcgt ggaggtggtc gcctaccacg ccgggctgcc caggcacgtc
     1861 cgcgccgacg tgcagcggcg cttcatggac ggctcggcgc ccgtcgtcgt cgccaccaac
     1921 gcgttcggca tgggcgtgga caaggccgac gtgcggaccg tctgccacga gtccgtcccg
     1981 tcctcgatcg aggcctacta ccaggaggcc ggtcgcgcgg ggcgcgacgg caagccggcg
     2041 cgcgcgctcc tgttctccac cagccgcgac aagggcctgc acgtgttctt catcgagcgc
     2101 gcggccgtcg aggacgatcg cctgcgggac gtcgcacggg ccatcgcccg gtcggcgacg
     2161 ggctccccgc cggtctaccg gctgcacatc aacgacctcg cgacggacga gagcgaggtg
     2221 gagtccgtcc gcgcgatcgt cggctacatg gtccgggcgg gcgtcgtcca gcccgcgccc
     2281 tcaggccccg accacgtcgc gggacgcctg gtgggcacct gggaccgtgg gtccctcgcc
     2341 gcctgccggg tggccgcgca ggagggcacg cgccagcgct ggcgccagta ccgcgccgtc
     2401 tgggcctggg tggagggcga gggctgccgc cgcgccgg
//
LOCUS       DASUPW010000253         3857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1303953, whole genome shotgun sequence.
ACCESSION   DASUPW010000253 DASUPW010000000
VERSION     DASUPW010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303953"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VFY44_05745"
     CDS             <1..394
                     /locus_tag="VFY44_05745"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEX5909976.1"
                     /translation="IWIGHPEAAAILMKLQGANTPRPMTHDLINDVLSELEVACTRVA
                     VTELRENTFYALITLQLGGRDVEIDSRPSDALALAVRTGAPIFAAEEVITESAIEFEH
                     EVEDQEEVVEKFKDFLDNVSPEDFGAES"
     gene            398..856
                     /locus_tag="VFY44_05750"
     CDS             398..856
                     /locus_tag="VFY44_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909977.1"
                     /translation="MPDEVEFGPPLPGDAQHATVRIDGDKARIEVRGLDGKTDKRKVT
                     ATEARAWIADVERRHREAAEWSARARERTDERRGEREALLTSLDLGCGRCGGTLAKGS
                     RVKLLAGDRPEQALRDEDFAAGRRSIVAYDEYACERCGAVQLFKPGFLAG"
     gene            complement(831..1787)
                     /locus_tag="VFY44_05755"
     CDS             complement(831..1787)
                     /locus_tag="VFY44_05755"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX5909978.1"
                     /translation="MNRVAVLKGGRSLERQVSLRSGANVEDALERLGHEVVSVDVGGD
                     LIRRLRDERPDVVFVALHGRDGEDGTIQELLEILDIPYTGSGVLACSRAIDKVMTKHL
                     LREEGIPTPDFVAFTQTAFRELGAADTLEAIEERLAFPIVVKPSRQGSSLGIKFASSA
                     ADVPGALVAAFSYDERVLLERHVQGRDLAVSVLEGPDGPEALPVVEAVPADGDPYDYE
                     SRYDIGRTTFLCPAELGEDVTARAQELALATYGLLGCYGFARVDLMLSEAGELSVLEA
                     NSIPGLTDTSLLPQAAEAAGIGFDQLVERIVELSFTRPGIPA"
     gene            complement(1784..3178)
                     /locus_tag="VFY44_05760"
     CDS             complement(1784..3178)
                     /locus_tag="VFY44_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX5909979.1"
                     /translation="MSPPSQPPSRSTKRDLERYAGLFAARTRVMTSSAMRDIMAVTAR
                     PEVISLAGGLPDTSTFPPETLAAVTARIAQESSAKALQYGPTDGLRETKECIAEVMAE
                     EGMGVDLDDVVVTTGGQQVIDLVVKTLIDPGDVIIAEGPTYPGAVPTFNAYQADVVHI
                     DMDSQGMRTDRLEETIDRIEAEGRRLKFIYTVPTFQNPGGATMSLERRRQLVRVAAER
                     EVLVLEDNPYGLLRYEGEALPPLYALDGGVYVMYLGTFSKILSPGIRLGWVSAPPPVL
                     EKINVGKQGADLCTSTLSQLMVQAYFERGDWREYVGSLTEVYRPRRDAMLDALEEFFP
                     AQAVWNRPQGGMFVWVTLPDFIDTTDLLARALRDNVAFVPGAGAFLDGRGHNSMRLNF
                     SASDEERTREGIRRIGKVITEQMELYGTLTGEMPAISTEELEVARQEDDAEAGARVVE
                     LRRKRDGRRRAEGA"
     gene            3286..>3857
                     /locus_tag="VFY44_05765"
     CDS             3286..>3857
                     /locus_tag="VFY44_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 family protein"
                     /protein_id="HEX5909980.1"
                     /translation="MSIVLDIGQGAGLASATGVRPFLPPLLAGALARGDIGIDFDDSG
                     WQFLESPAFLVIVLALAVAAYLAERGGVDRRTLLIATTTVAAVLGGMLFAGSLAAGDG
                     PPVAGLVGGVACAVLAALAVGGVFERAAKRLDDSAAGLLLVYADIAALVLAAIAIFVP
                     PVAFLAVIAFVVLLIRGSGGGDEKYAGLRIL"
BASE COUNT          515 a         1340 c         1446 g          556 t
ORIGIN      
        1 gatctggatc ggtcatcccg aggcggcggc catcctcatg aagctccagg gcgcgaacac
       61 cccgcggccg atgacgcacg acctgatcaa cgacgtgttg tcggagctcg aggtggcgtg
      121 cacccgtgtg gcggtgaccg agctgcgcga gaacaccttc tacgccctga tcaccctgca
      181 gctgggcgga cgggacgtgg agatcgactc gcgcccgtcg gacgcgctgg cgctggccgt
      241 gcgcaccggc gccccgatct tcgcggccga ggaggtcatc accgagtcgg cgatcgagtt
      301 cgagcacgag gtcgaggacc aggaggaggt cgtcgagaag ttcaaggact tcctcgacaa
      361 cgtctctccc gaggacttcg gcgccgagtc gtaggccgtg ccggacgagg tcgagttcgg
      421 cccgcccctg cccggcgacg cgcagcacgc gacggtccgg atcgacggcg acaaggcgcg
      481 gatcgaggtc cggggcctgg atggcaagac cgacaagcgc aaggtcacgg cgaccgaggc
      541 gcgagcgtgg atcgcggacg tggagcgccg ccaccgcgag gccgcggagt ggagtgcgcg
      601 cgcgagggag cgcaccgacg agcgtcgggg cgagcgcgag gcgctgctga cctcgctgga
      661 cctgggctgc ggacgctgcg gcgggacgct ggccaagggg tcacgcgtga aactgctggc
      721 cggcgaccgc cccgagcagg cgctacgcga cgaggacttc gccgctggac ggcgctcgat
      781 cgtggcctac gacgagtacg cgtgcgagcg ctgtggcgcc gtgcagctgt tcaagccggg
      841 attcctggcc gggtgaacga gagctcgacg atccgctcga cgagctggtc gaacccgatg
      901 ccggccgcct cggcggcctg ggggaggagg ctggtgtcgg tcaggccggg gatggagttg
      961 gcctccagca ccgacagctc gccggcctcg gagagcatca ggtccacgcg cgcgaagccg
     1021 taacacccga gcagcccgta ggtggccagc gccagctcct gggcccgcgc ggtgacgtcc
     1081 tcgcccagct ccgccgggca caggaaggtg gtgcggccga tgtcgtaccg cgactcgtag
     1141 tcgtaggggt cgccgtcggc gggcacggcc tcgacgaccg ggagcgcctc gggcccgtcc
     1201 ggcccctcga gcacggacac ggcgaggtcg cggccctgga cgtggcgttc cagcagcacg
     1261 cgctcgtcgt aggagaaggc ggccacgagc gcgccgggca cgtcggcggc ggagctggcg
     1321 aacttgatgc ccagcgacga tccctggcgc gagggcttga ccacgatcgg gaacgccagc
     1381 cgctcctcga tggcctcgag ggtgtccgcc gcgcccagct cgcggaacgc ggtctgggtg
     1441 aaggccacga agtccggcgt ggggatcccc tcctcccgga ggaggtgttt ggtcatgacc
     1501 ttgtcgatgg cccgcgagca ggccagcacg cccgagccgg tgtacggaat gtccaggatt
     1561 tccaggagtt cctggatcgt cccgtcctcg ccgtcgcggc cgtgcagcgc gacgaacacg
     1621 acgtccgggc gctcgtcgcg cagccgccgg atcaggtcgc cgcccacgtc gaccgagacc
     1681 acctcgtggc ccaggcgctc gagcgcgtcc tccacgttgg cgccggaccg cagcgacacc
     1741 tgccgctcga gtgagcgccc gcccttcaga accgcgacgc ggttcacgcg ccctcggccc
     1801 ggcgccggcc gtcgcgcttg cggcgcagct cgacgacccg ggccccggcc tcggcgtcgt
     1861 cctcctggcg ggcgacctcg agctcctcgg tgctgatggc gggcatctcg ccggtgagcg
     1921 tgccgtacag ctccatctgc tcggtgatga ccttgccgat gcggcggatg ccctcgcgcg
     1981 tgcgctcctc gtcggaggcg gagaagttca gccgcatcga gttgtggccg cggccgtcca
     2041 ggaaggcgcc ggcgccgggc acgaacgcga cgttgtcgcg cagggcgcgg gccaggaggt
     2101 cggtggtgtc gatgaagtcg ggcagcgtga cccacacgaa catcccgccc tggggccggt
     2161 tccacaccgc ctgggcgggg aagaactcct ccagcgcgtc cagcatcgcg tcgcggcggg
     2221 ggcggtagac ctcggtcagc gaccccacgt actcgcgcca gtcgccgcgc tcgaagtagg
     2281 cctgcaccat cagctgcgac agcgtcgacg tgcacaggtc ggcgccctgc ttgccgacgt
     2341 tgatcttctc gagcaccggt ggcggcgcgg acacccagcc cagccggatg ccgggcgaga
     2401 ggatcttcga gaacgtgccc aggtacatca cgtagacgcc gccgtccagg gcgtacagcg
     2461 gcggcagcgc ctcgccctcg tagcgcagca ggccgtaggg gttgtcctcc aggaccagca
     2521 cctcgcgctc ggccgcgacc cgcacgagct ggcgccgccg ctcgagcgac atcgtggcgc
     2581 cgcccgggtt ctggaacgtg ggcacggtgt agatgaactt cagccgccgg ccctcggcct
     2641 cgatccggtc gatcgtctcc tccaggcggt cggtgcgcat gccctgggag tccatgtcga
     2701 tgtggaccac gtccgcctgg taggcgttga aggtggggac ggccccgggg taggtggggc
     2761 cctcggcgat gatcacgtcg cccgggtcga tcagggtctt gaccaccagg tcgatcacct
     2821 gctggccgcc ggtggtgacg accacgtcgt ccaggtcgac gcccatgccc tcctcggcca
     2881 tgacctcggc gatgcactcc ttggtctcgc gcaggccgtc ggtggggccg tactgcagcg
     2941 ccttggccga cgactcctgg gcgatcctgg cggtgacggc ggccagggtc tccggcggga
     3001 aggtggatgt gtcgggcagc cctccggcca gcgagatcac ctcgggccgg gcggtcacgg
     3061 ccatgatgtc gcgcatggcc gaggacgtca tgacccgcgt gcgcgccgcg aacaggccgg
     3121 cgtagcgttc gaggtcgcgc ttggtggagc gcgacggggg ctgtgacggg gggctcatgg
     3181 tgcgtcgttc gccgccgcgg cgggcggtcc tggcgccgag ccgggccgcc cccgtggtcg
     3241 tctatcgtcc ggtcgcagcg taccggtccc gcggggcctc cagccatgag catcgtcctc
     3301 gacatcggcc aaggcgcagg cctggcatcc gccacgggcg tgcgcccgtt cctgcccccg
     3361 ctcctggcgg gagcgctcgc gcggggcgac atcgggatcg acttcgacga cagcggatgg
     3421 cagttcctgg agagcccggc cttcctggtg atcgtcctgg ccctggccgt ggcggcctac
     3481 ctggccgagc gtggcggcgt ggaccgccgc acgctgctca tcgcgaccac gaccgtggcc
     3541 gcggtgctgg gcgggatgct gttcgccggc tcgctggccg cgggcgacgg cccgccggtc
     3601 gccggcctcg tgggcggcgt cgcgtgcgcc gtgctggcgg cgctggccgt ggggggcgtg
     3661 ttcgagcgcg ccgcgaagcg gttggacgac tcggccgccg ggctgctcct ggtgtacgcc
     3721 gacatcgcgg cgctggtgct cgcggccatc gcgatcttcg tcccgccggt ggcgttcctg
     3781 gccgtgatcg ccttcgtggt gctgctcatc cggggcagcg gcggcggcga cgagaagtac
     3841 gccggcctgc ggatcct
//
LOCUS       DASUPW010000254         2963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_336128, whole genome shotgun sequence.
ACCESSION   DASUPW010000254 DASUPW010000000
VERSION     DASUPW010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336128"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="VFY44_05770"
     CDS             complement(<1..1089)
                     /locus_tag="VFY44_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909981.1"
                     /translation="MRSPARIAAAALLASPGVLVVFLSFSGGGYFPNVYGLGAAAMAV
                     ALILRVTLARRPFAGLSGISMAVIVLLTLFAGWTLASALWSDTGGRAFLEFERALLYL
                     LAFILFASIPQSSDRLRWMLRGVAAGLVIVCAVALTTRVAADVWPIGPNIAGSRLSYP
                     VTYWNALGLMAAGAGLLCLHLTADEREPLAVRALGAAALPVVLCTLLFTFSRGAIVAG
                     VVGLVLYVVVAHPRGLLGALLATLVPVGFALKAAYDAELLQSESFASAAAVDQGHEVA
                     LVVIGAALVAGLLRIAVSPLDTRLARVRVPRVPRAAQAGLAALAVAVVAGAAVAAGAS
                     GYVSRQYDGFVNGGGLKNDEAQLRSRLTN"
     gene            1183..2130
                     /locus_tag="VFY44_05775"
     CDS             1183..2130
                     /locus_tag="VFY44_05775"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5909982.1"
                     /translation="MPTLLAASAGGHLDELIRLRPRLGPLGDDVVWVTFETPQSRSRL
                     ADENVVFLPLIAPRDYQALARSLPRARKLLSEIAPEAVVSTGSAIALSLIPLARARGI
                     PCHYVESAARSDGPSLTGRLLAKVHGVHLYTQYADWTGKGWHYAGSVFDGFAPGPRLE
                     LADVSRVVVTLGMIKPYGFESLVEKLLTLLPPTAEVLWQTGSTDVSRYPIEAHPTLPA
                     RELDAAMREADLVIAHSGIGSSLAAIEAGHAPLLVPRRGHRGEHVDDHQQLIAADLAE
                     RGLAIAREVDELTFDDVAYAAQARVQRVEASPPLMLAVA"
     gene            2151..>2963
                     /locus_tag="VFY44_05780"
     CDS             2151..>2963
                     /locus_tag="VFY44_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909983.1"
                     /translation="MVGSDNTPMTVCFDLDGTLCTNTYGAYDEAEPFPWAIARVNALA
                     EAGHRIIVFTARGTATGLDWRETTEGQLQRWGVRYDELQLGKPSAAVYVDDRAVHTTA
                     WREGDAWAVPGFGVPAPEGRREPLPEPLALQRTTVVESGRTYGGVPFLAEEHVDRVLR
                     RAAGAGIAELPEPRELTTALDTALARARAAGEPDVVFTFAVADGVAPGFLDVADERAG
                     GGVVTVRSIEQARAGLAGSPATLGGRRAAAIDGRLVLGDAGDRPGPLGALLEE"
BASE COUNT          421 a         1108 c         1086 g          348 t
ORIGIN      
        1 gttggtcagc cgcgagcgca gctgggcctc gtcgttcttg aggccgccgc cgttcacgaa
       61 cccgtcgtac tggcgcgaga cgtacccgga ggcccccgcc gccaccgcgg ccccggcgac
      121 caccgccacg gccagggcgg ccaggccggc ctgcgccgcg cgcgggaccc gcggcacgcg
      181 aacgcgggcc agccgggtgt ccaggggcga gacggcgatc cgcagcagcc cggccaccag
      241 cgcggcgccg atcaccacga gggccacctc gtggccctgg tccacggcgg ccgccgacgc
      301 gaagctctcg ctctgcagca gctcggcgtc gtacgcggcc ttgagcgcga agcccaccgg
      361 cacgagggtg gccagcagcg cgccgagcag cccgcgggga tgggcgacca ccacgtacag
      421 cacgaggccg accacgccgg cgacgatcgc cccgcgcgag aaggtgaaca gcagcgtgca
      481 caggaccacc ggcagcgctg ccgcgcccag cgcccgcacg gccaggggct cgcgctcgtc
      541 ggcggtcaga tgcaggcaca gcagcccggc gcccgcggcc atcaggccca gggcgttcca
      601 gtaggtgacc ggatagctca gccggctgcc ggcgatgttg ggcccgatcg gccacacgtc
      661 ggcggccacc cgcgtggtca gggcgacggc gcagacgatc acgagcccgg cggcgacgcc
      721 gcgcagcatc cagcgcagcc ggtcggagga ctgcgggatc gacgcgaaca ggatgaacgc
      781 cagcaggtac agcagggcgc gctcgaactc caggaaggcc cgcccgccgg tgtccgacca
      841 gagggccgag gccagcgtcc agccggcgaa cagcgtcagc agcacgatca ccgccatcga
      901 gatcccgctg aggccggcga acggccgccg cgccagcgtc acgcgcagga tcagcgccac
      961 cgccatcgcc gccgccccca gcccgtagac gttggggaag tatccgccgc ccgagaacga
     1021 caggaacacc accagcacgc cgggggacgc cagcagcgcg gccgcggcga tgcgtgctgg
     1081 cgatctcacc ggcggcatca taggaacagt gccgtcccgg tcggcgctcc ttgtcccgcg
     1141 aacgggtggc gcggtcgtgg ctgctggctt acgctggatc tggtgcccac gctgttggcc
     1201 gcctccgccg gcggacatct tgacgagctg atccgcctga ggcctcgcct cgggccactc
     1261 ggcgacgacg tggtctgggt gaccttcgag acgccgcaga gccggtcccg gctcgccgac
     1321 gagaacgtcg tcttcctgcc cctgatcgcg ccgagggact accaggcgct ggcccggagc
     1381 cttccccggg cccgcaagct cctgagcgag atcgccccgg aggccgtggt gagcaccgga
     1441 tcggcgatcg cgctgagcct gatccccctg gcccgggcgc gcggcatccc gtgtcactac
     1501 gtggagagcg ccgcccgcag cgacgggccg tcgttgaccg gccggctgct cgccaaggtg
     1561 cacggggtcc acctgtacac ccagtacgcg gactggacgg gcaagggctg gcactacgcc
     1621 ggttcggtct tcgacggctt cgcccccggg ccgaggctcg agctggccga cgtgagccgg
     1681 gtcgtggtga cgctggggat gatcaagccg tacgggttcg agagcctggt cgagaagctg
     1741 ctgaccctcc tccctcccac ggccgaggtc ctgtggcaga ccggctcgac cgacgtctcc
     1801 cggtacccca tcgaggcgca tcccaccctc cccgccaggg agctggacgc ggcgatgcgt
     1861 gaggcggacc tggtgatcgc ccattcgggt atcgggtcga gcctggccgc gatcgaagcc
     1921 ggccacgcgc ccctcctggt gccacgccgg ggccacaggg gcgagcacgt cgacgaccac
     1981 cagcagttga tcgcggccga tctggccgaa cgcggcctgg ccatcgcgcg cgaggtggat
     2041 gagctcacgt tcgacgatgt ggcgtatgcg gctcaggcca gggtccagcg ggtggaggcg
     2101 tctccgcctc tgatgctggc cgtcgcctga cctccgatag gttcgccccc ttggtcggca
     2161 gtgacaacac gccaatgacc gtctgcttcg acctggacgg gacgctgtgc acgaacacgt
     2221 acggcgccta cgacgaggcg gagccgttcc cgtgggcgat cgcacgcgtg aacgcgctgg
     2281 ccgaggcggg ccaccggatc atcgtcttca cggcccgcgg caccgccacc ggcctggact
     2341 ggcgtgagac caccgagggc cagcttcagc gctggggcgt gcgctacgac gagctccagc
     2401 tgggcaagcc cagcgccgcg gtgtacgtgg acgaccgtgc cgtgcacacc accgcctggc
     2461 gcgagggcga cgcctgggcg gtccccggct tcggcgtccc cgcccccgag ggcaggcgtg
     2521 agccgctgcc cgagccgctg gcgctacagc gcaccacggt ggtcgagagc ggccgcacct
     2581 acggcggcgt cccgttcctg gccgaagagc acgtggaccg ggtcctgcgg cgcgccgccg
     2641 gcgccggcat cgccgagctg cccgagcccc gggagctgac gaccgcgctg gacaccgccc
     2701 tggcccgggc gcgcgccgcc ggtgagcccg acgtggtctt caccttcgcc gtggccgacg
     2761 gcgtggcccc gggcttcctg gacgtggcgg acgagcgggc cggcggcggc gtggtgacgg
     2821 tgcgcagcat cgagcaggcg cgggccgggc tggcgggcag ccccgccacc ctgggcgggc
     2881 ggcgggcggc cgcgatcgac gggcgcctgg tcctcggcga cgccggcgac cgcccggggc
     2941 cgctcggtgc cctgctcgag gag
//
LOCUS       DASUPW010000255         4921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_611821, whole genome shotgun sequence.
ACCESSION   DASUPW010000255 DASUPW010000000
VERSION     DASUPW010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611821"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1715)
                     /locus_tag="VFY44_05785"
     CDS             complement(<1..1715)
                     /locus_tag="VFY44_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HEX5909984.1"
                     /translation="MDPAARTHPPALNGLPRASGLYDPAHEHDGCGVACLARLDGKVT
                     RETVERAIGALVNLEHRGAEGADPSTGDGAGILMQIPDAFMRAEVHPDLPPAGHYGIA
                     FCFLPQEPGRCKEVEVLLEGIAREEGLEPITWRDVPVEPGEAGERSREVAPTMRQLFM
                     DAGSLEGEDLERKLYVVRRRGENESGGDLYLPSCSSRTIVYKGMLSAPQLARYYPDLA
                     DERLASALAIVHSRFSTNTFPSWALAHPFHMSAHNGEFNTLLGNVSWMRAREARLAAG
                     PLGDDVARTLPAIPVGSSDSAAFDAVLELLVRGGRTLEHSVMLMTPEAHEGRPDMPGP
                     LSDFYDYGSMLMEPWDGPASVTFTDGKILGATLDRNGLRPGRWVVTRDGWFALASEAG
                     AFHVPSEDVERVGRLLPGRLLVVDPERGGLLEDRQAELDVAGDRPYGAWFAEHSLGID
                     DLPSARPQERPAFSREQRQLAFGWTQEDVRSVLAPMAGGAEAVGSMGNDVALAAISER
                     QPPLFTYFKQLFAQVTNPAIDPVREKIVMSLRTVVGPEGDLFEETPEQARRLVLEHPV
                     INDEDL"
     gene            1907..4435
                     /gene="clpB"
                     /locus_tag="VFY44_05790"
     CDS             1907..4435
                     /gene="clpB"
                     /locus_tag="VFY44_05790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEX5909985.1"
                     /translation="MTVKTQEALAAAQRLAQTRRNAQVGPHHLLVALLEQDGGIVVPV
                     LRRAGADPDAVRRKANQALDEAPTVTGDTSAQPTPDASLGELFAKADETARGLGDEYV
                     STEHLLLALAADPKIDVGASAEQLAEAVQGVRGPHRVTDQNPEDRYQALERFGRDLTE
                     AAEQGKLDPVIGRDDEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIAEGLAQRIVSGDV
                     PDSLRDRRVIALDIGSLIAGAKYRGEFEDRLKAVLKEIADAEGQVILFMDELHTIVGA
                     GAAEGAVDAANLLKPMLARGELRAVGATTLDEYRKHIEKDAALERRFQPVHVGEPSVE
                     ATIAILRGLKERYEVHHGVRVQDSAIVAAATLSHRYIADRFLPDKAIDLIDEAASRLR
                     IEIDSMPEEIEVVERRIQQLEIEHQALKKEKDESSVARREAIERELADLREESSEKKA
                     HWQGEKEAIDAIRAAKEELEQAHRDVERAEREADLERAAELRYGRIPELQQLVADTEA
                     SLRELQSEHTYLKEEVDDEDVAEVVARWTNIPVSRLMESEVAKLVHMEERLHDRVIGQ
                     EEAVEAVSNALRRSRAGLQDPDQPIGSFLFIGPTGVGKTELARALAEFMFDSQDAMVR
                     IDMSEYMEKHSVSRLVGAPPGYVGYDEGGQLTEAVRRRPYSVVLLDEIEKAHTDVFNV
                     LLQLMDDGRLTDGQGRTVNFTNTVVIMTSNVAGGIAGVEATFKPEFVNRLDEVVEFAA
                     LTREQIGQIVELQVDQLIARVRERGVEVELTDAAKELLGNLGYDPTYGARPLKRVIQK
                     RLVDKLALKLLENEFVPGDTVRVDAEEGELTFTGVDIAHGTPVA"
     gene            4416..4769
                     /locus_tag="VFY44_05795"
     CDS             4416..4769
                     /locus_tag="VFY44_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909986.1"
                     /translation="MGRRSRKRAQQGGGAPAATSDYTADDGSVLTLRDQLSAGTRKKL
                     GDLDAKPAADAEDRWQRRVEFLFERLAVRWVIADLPLEGQKELLGRYRMASSEERQRV
                     RQALDAHLADHYPDL"
BASE COUNT          763 a         1717 c         1810 g          631 t
ORIGIN      
        1 aggtcctcgt cgttgatcac cgggtgctcc agcaccaggc gccgcgcctg ctcgggcgtc
       61 tcctcgaaca ggtcgccctc ggggccgacc acggtgcgca ggctcatcac gatcttctcg
      121 cgcacggggt cgatggccgg gttggtgacc tgcgcgaaca gctgcttgaa gtaggtgaac
      181 agcggcggct ggcgctcgga gatggcggcc agcgccacgt cgttgcccat ggagcccacg
      241 gcctcggcgc cgccggccat gggggccagc accgagcgca cgtcctcctg ggtccagccg
      301 aaggccagct ggcgctgctc gcgcgagaac gcgggccgct cctgcggccg ggcggagggc
      361 aggtcgtcga tgcccagcga gtgctcggcg aaccacgcgc cgtagggccg gtcgccggcc
      421 acgtccagct cggcctggcg gtcctccagc aggccgcccc gctcggggtc cacgaccagc
      481 aggcggccgg gcagcagccg cccgacgcgc tcgacgtcct cgctgggcac gtggaaggcg
      541 cccgcctccg aggccagcgc gaaccagccg tcgcgggtga ccacccagcg gcccgggcgc
      601 aggccgttgc ggtccagcgt ggcgcccagg atcttgccgt cggtgaacgt gaccgacgcg
      661 ggcccgtccc acggctccat gagcatcgag ccgtagtcgt agaagtcgct gagcgggccg
      721 ggcatgtccg gccggccctc gtgcgcctcg ggcgtcatca gcatcaccga gtgctcgagc
      781 gtgcgcccgc cgcggaccag cagctcgagc acggcgtcga acgcggccga gtccgagctg
      841 cccacgggga tggccggcag ggtccgggcc acgtcgtcgc ccagcgggcc ggcggccagg
      901 cgcgcctcgc gcgcccgcat ccagctgacg ttgcccagca gcgtgttgaa ctcgccgttg
      961 tgggcgctca tgtggaacgg gtgcgccagc gcccagctgg ggaaggtgtt ggtcgagaag
     1021 cgcgagtgca cgatggccag ggcgctggcc aggcgctcgt cggccaggtc cgggtagtag
     1081 cgcgccagct gcggcgcgga gagcatgccc ttgtagacga tggtgcgcga cgagcagctg
     1141 ggcaggtaga ggtcgccgcc cgactcgttc tcgccccggc ggcggaccac gtagagcttg
     1201 cgctccaggt cctcgccctc cagcgagccg gcgtccatga acagctggcg catggtgggc
     1261 gccacctcgc gtgagcgctc gccggcctcg cccggctcca ccgggacgtc gcgccaggtg
     1321 atgggctcca ggccctcctc gcgtgcgatg ccctcgagca ggacctcgac ctccttgcag
     1381 cggccgggct cctggggcag gaagcagaag gcgatgccgt agtggccggc gggcggcagg
     1441 tcggggtgca cctcggcgcg catgaacgcg tcggggatct gcatcaggat gccggcgccg
     1501 tcgccggtcg acgggtccgc gccctcggcc ccgcggtgct ccaggttcac cagtgcgccg
     1561 atggcgcgct cgaccgtctc gcgcgtgacc ttgccgtcca gccgggccag gcaggcgacg
     1621 ccacagccgt cgtgctcgtg cgccgggtcg tacagacccg acgcgcgggg gagcccgttc
     1681 agggcaggag gatgtgtgcg ggccgcgggg tccaccggtg aaagagtacg gacgtcggcc
     1741 ggaaaacggc agtgccggat gtccaaagcg gtcgtccact tggtccaggg ttcggccccg
     1801 aatctctttg gacatccgcg ccgtgtccct ctaatctaag tgtcacagac ttagatttat
     1861 ccgaccgcga cagaccgagg aacccacgcc gatgcagcca gaccggatga ccgtgaagac
     1921 acaggaggcc ctcgccgccg cgcagcggct ggcccagacg cgccgcaacg cccaggtggg
     1981 cccccaccac ctgctggtgg cgctgctcga gcaggacggc ggcatcgtcg tgcccgtgct
     2041 ccggcgtgcc ggggcggacc ccgacgcagt gcgccgcaag gcgaaccagg ccctggacga
     2101 ggcccccacg gtcaccggcg acacctcggc ccagcccacc cccgacgcgt cgctgggcga
     2161 gctgttcgcc aaggccgacg agacggcgcg cggcctgggc gacgagtacg tgtccaccga
     2221 gcacctcctg ctggcgttgg ccgccgaccc caagatcgac gtgggcgcgt cggccgagca
     2281 gctggccgag gccgtccagg gcgtgcgcgg cccgcaccgc gtgaccgacc agaaccccga
     2341 ggaccgctac caggcgctcg agcgcttcgg ccgcgacctg accgaggcgg ccgagcaggg
     2401 aaagctcgac ccggtcatcg gccgcgacga cgagatccgg cgcgtgatcc aggtgctgtc
     2461 gcgccgcacc aagaacaacc ccgtcctgat cggcgagccc ggcgtgggca agaccgccat
     2521 cgccgagggg ctggcccagc ggatcgtgtc cggcgacgtg cccgactcgc tgcgcgaccg
     2581 gcgcgtgatc gcgctggaca tcggctcgct gatcgccggc gccaagtacc gcggcgagtt
     2641 cgaggaccgg ctcaaggccg tgctcaagga gatcgccgat gccgagggcc aggtgatcct
     2701 gttcatggac gagctgcaca ccatcgtcgg cgccggcgcg gccgagggcg ccgtggacgc
     2761 cgccaacctg ctgaagccga tgctggcgcg cggcgagctg cgcgcggtcg gcgcgaccac
     2821 cctggacgag taccgcaagc acatcgagaa ggacgccgcc ctggagcgcc ggttccagcc
     2881 ggtgcacgtc ggcgagccgt cggtcgaggc caccatcgcc atcctgcgcg ggctcaagga
     2941 gcgctacgag gtccaccacg gcgtgcgggt ccaggactcg gcgatcgtcg cggcggccac
     3001 gctgtcgcac cgctacatcg ccgaccgctt cctgcccgac aaggcgatcg acctgatcga
     3061 cgaggccgcc tcgcggctgc ggatcgagat cgactcgatg ccggaggaga tcgaggtcgt
     3121 cgagcgccgc atccagcagc tggagatcga gcaccaggcg ctcaagaagg agaaggacga
     3181 gtccagcgtg gcccgccgcg aggccatcga gcgtgagctg gccgacctgc gtgaggagtc
     3241 gtcggagaag aaggcccact ggcagggcga gaaggaggcc atcgacgcga tccgcgccgc
     3301 caaggaagag ctggagcagg cccaccgcga cgtcgagcgg gccgagcgcg aagccgacct
     3361 ggagcgtgcg gccgagctgc gctacggcag gattcccgag ctccagcagc tcgtggccga
     3421 caccgaggcg agcctgcgcg agctgcagtc cgagcacacc tacctcaagg aggaggtcga
     3481 cgacgaggac gttgccgagg tcgtggcgcg ctggaccaac atccccgtct cgcggctgat
     3541 ggagtccgag gtggcgaagc tggtgcacat ggaggagcgc ctgcacgacc gggtgatcgg
     3601 ccaggaggag gcggtcgagg cggtgtccaa cgcgcttcgg cgctcacgcg ccgggctcca
     3661 ggaccccgac cagccgatcg gctcgttcct gttcatcggc cccacgggcg tgggcaagac
     3721 cgagctggcg cgcgcgctgg ccgagttcat gttcgactcc caggacgcga tggtccgcat
     3781 cgacatgtcg gagtacatgg agaagcactc ggtgtcgcgg ctggtgggcg cgccccccgg
     3841 ctacgtgggc tacgacgagg gcgggcagct gaccgaggcc gtgcgccggc gcccgtacag
     3901 cgtcgtgctg ctggacgaga tcgagaaggc gcacaccgac gtgttcaacg tgctgctgca
     3961 gctcatggac gacggtcgcc tgaccgacgg ccagggccgt acggtcaact tcaccaacac
     4021 cgtggtgatc atgacctcca acgtggccgg cgggatcgcg ggcgtggagg caaccttcaa
     4081 gcccgagttc gtgaaccggc tggacgaggt ggtcgagttc gccgccctga cgcgcgagca
     4141 gatcggccag atcgtcgagc tccaggtgga ccagctgatc gcccgcgtgc gcgagcgcgg
     4201 cgtggaggtc gagctgaccg acgccgccaa ggagctgctg ggcaacctgg gctacgaccc
     4261 cacgtacggc gcccggccgc tcaagcgcgt gatccagaag cgcctggtcg acaagctggc
     4321 gctgaagctc ctggagaacg agttcgtgcc cggcgacacc gtgcgcgtgg acgccgagga
     4381 gggcgagctg accttcaccg gtgtagacat cgcgcatggg acgccggtcg cgtaagcggg
     4441 cacagcaggg cgggggagcc cccgccgcca cgtcggacta caccgccgac gacggcagcg
     4501 tgctgacgct gcgcgaccag ctcagcgccg gcacgcgcaa gaagctgggt gacctggacg
     4561 ccaagcccgc cgcggacgcc gaggaccgct ggcagcgccg ggtcgagttc ctgttcgagc
     4621 gcctggccgt gcgctgggtg atcgcggacc tgccgctgga gggccagaag gagctgctgg
     4681 gccgctaccg gatggcctcg tccgaggagc ggcagcgggt ccgccaggcg ctggatgcgc
     4741 acctggcgga ccactacccc gatctctagg ggagctgcgt ccagacctcg ccggtggagg
     4801 ccgccgtggc cgagttggcc cggcgcacga tccggtagac gcgcacctgg aagcggacgc
     4861 gctgcagcgt cttcgtgctg taggagcgct tcgtgtcgaa ctgcacccgg taggtgccgc
     4921 t
//
LOCUS       DASUPW010000256         2909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_474935, whole genome shotgun sequence.
ACCESSION   DASUPW010000256 DASUPW010000000
VERSION     DASUPW010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474935"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VFY44_05800"
     CDS             complement(<1..687)
                     /locus_tag="VFY44_05800"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5909987.1"
                     /translation="MAVLLGFLGVLGFSGSLPATRVAVEGLDPTFVGVGRAVVAGLLA
                     AALLAATRQPWPRRAQVRPLAMVALGVVFGFPLFTALAMHSLPSAQGAVIVGVLPAAT
                     AVAAVLFAGERPSGAFWAASGAGLVAVLVFAGAQGAGLPSGAHGLVLIAVALCALGYA
                     KGGALSRELGGWQTICWALVLSLPLTIPVTVVTLLVGDLSAGADAWAGFAYVSLISMF
                     LGFFAWYAGLA"
     gene            771..1991
                     /locus_tag="VFY44_05805"
     CDS             771..1991
                     /locus_tag="VFY44_05805"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5909988.1"
                     /translation="MAVPAVLQPGEVIGGRYEIVAPISSGAMGAVFRARADGREVALK
                     LLTNPGQATRFDIEARLLGRLSHPRVVDVVDHGRDDGRDWLVMELVEGPDLGRVLSER
                     GAPGLPAADVLEAARQAAEALEYVHGQQIVHRDVKPHNLLMAGDGVVLVDFGVAREVT
                     AEAGTRGIGTPRFMAPEVLVGEGVSPRSDVYGLAATVWMLLSGSPPAYGDPAPLPEVA
                     PELEQALRRALDPRPEHRFASAAALAAALGSPVRTAAGRTLAATLPGTEHAGVLESVV
                     RTAAGVFDAAAASIALIDETTGELVYRSSWGVLAEETVGIRLAPGQGLAGAAVAAMEP
                     VVVPDCRADERFAAQVAAGIGYVPHTMLVVPLQRDGVAVGALSILDRRDGEAMDQADV
                     ARAALFADLALAVL"
     gene            1988..2893
                     /locus_tag="VFY44_05810"
     CDS             1988..2893
                     /locus_tag="VFY44_05810"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5909989.1"
                     /translation="MSGYDSVLLPGDHFAGYLVDLELGRGGMGRVYRATREADGAEVA
                     LKLLPPELAGEDRYRRRFARESRLAASLSHPHLVPVLDSGEHEGALWIAMQLIDGRDL
                     GAVLTEQGTIHPANAARIVAQVGGGLDAAAGEGLVHRDVKPANVFVVARDGHPHAYLG
                     DFGLSKATDSQSGLTATGKFLGTVAYAAPEQAQGEPVGPATDVYQLGGLLYRALSGSQ
                     PFPRPRQIATVMAHIKDPPPRPSEAEPACPKAFDRVVATAMAKKPGDRYPTASALGEA
                     ALAAAEGAGPPPGWPEAGTFDPVPD"
BASE COUNT          389 a         1096 c         1115 g          309 t
ORIGIN      
        1 ggccagcccg gcgtaccagg cgaagaagcc caggaacatg ctgatcagcg aaacgtaggc
       61 gaagccggcc caggcgtcgg cgccggcgga caggtcgccc acgagcaggg tgacgaccgt
      121 gaccgggatc gtcagcggca gcgacagcac cagcgcccag cagatggtct gccagccccc
      181 gagctcccgg gacagcgccc cgcccttcgc gtagcccagc gcgcacaggg cgacggcgat
      241 cagcaccagc ccgtgcgccc cgctcggcag gcccgcgccc tgcgcgccgg cgaacaccag
      301 gacggccacc agccccgccc ccgacgccgc ccagaacgcg ccgctgggcc gctcgcccgc
      361 gaacagcacg gcggcgacgg ccgtggccgc gggcagcacg ccgacgatga ccgccccctg
      421 cgcggagggc agcgagtgca tggccagcgc ggtgaacagc gggaagccga acaccacccc
      481 cagggcgacc atggcgagcg ggcgcacctg cgcgcgacgt ggccacggct gccgggtagc
      541 ggccagcagc gcggcggcca gcagaccggc gaccacggcg cggccaacac cgacgaacgt
      601 ggggtccagt ccctcgaccg cgacgcgcgt ggcgggcagc gagccgctga agcccagaac
      661 accgaggaag cccaggagca ccgccacgcc ggcgacgcta cgcccgggag ggcgccggcg
      721 gaacgtccta caaccgggtc ttgtgcgacg aggcgggtta cgctggcgca gtggcggtac
      781 ccgcggtact gcagccgggt gaggtgatcg gagggcgcta cgagatcgtc gcccccatct
      841 cctcgggcgc catgggcgcc gtcttccgcg cccgcgccga cggccgcgag gtcgcgctca
      901 agctgctgac caacccgggc caggcgaccc ggttcgacat cgaggcccgc ctgctggggc
      961 gcctgagcca cccgcgcgtg gtggacgtcg tcgaccacgg ccgggacgac ggccgcgact
     1021 ggctggtcat ggagctggtc gaggggcccg acctgggccg ggtcctgagc gagcggggcg
     1081 cgcccgggct gccggcggcc gacgtgctcg aggcggcgcg ccaggccgcc gaggcgctgg
     1141 agtacgtgca cggccagcag atcgtccacc gtgacgtgaa gccccacaac ctcctgatgg
     1201 ccggcgacgg ggtcgtgctg gtggacttcg gcgtggcccg cgaggtcacc gccgaggcgg
     1261 gcacgcgcgg gatcgggaca ccgcggttca tggcccccga ggtgctcgtg ggcgaagggg
     1321 tgtcgccgcg cagcgacgtc tacggcctgg ccgcgaccgt gtggatgctg ctgtccggat
     1381 cgccgcccgc gtacggcgat ccggcgccgc tgcccgaggt ggccccggag ctggagcagg
     1441 cgctgcggcg cgcgctcgac ccgcggcccg agcaccggtt cgcctccgcg gccgcgctgg
     1501 ccgcagcgct gggctccccg gtccggacgg cggccgggcg cacgctggcc gccaccctgc
     1561 ccggcaccga gcacgccggc gtgctggagt cggtggtgcg cacggcggcg ggcgtgttcg
     1621 acgccgccgc ggcctcgatc gcgctgatcg atgagaccac cggcgagctg gtctaccgct
     1681 cctcctgggg cgtgctggcg gaggagacgg tgggcatccg gctggcgccc gggcagggcc
     1741 tggcgggggc ggcggtcgcc gcgatggagc cggtggtggt cccggactgc cgcgccgacg
     1801 agcgcttcgc cgcccaggtg gccgccggga tcggctacgt gccgcacacc atgctggtgg
     1861 tgcccctcca gcgcgacggc gtcgcggtcg gcgcgctgtc gatcctcgac cgccgcgacg
     1921 gcgaggccat ggaccaggcc gacgtggccc gggccgccct gttcgccgac ctggcgctgg
     1981 cggtcctgtg agcggctacg acagcgtcct gctgcccggc gaccacttcg ccggctacct
     2041 ggtggacctc gagctggggc gcggcggcat gggccgcgtg taccgcgcca cccgcgaggc
     2101 cgacggcgcg gaggtcgcgc tcaagctcct gccgcccgag ctggccggcg aggaccgcta
     2161 ccggcgccgg ttcgcgcgcg agtcgcggct ggcggcgtcg ctctcgcacc cccacctggt
     2221 gccggtgctg gactccggcg agcacgaggg cgcgctgtgg atcgccatgc agctgatcga
     2281 cggccgggac ctgggggcgg tgctgaccga gcagggcacc atccacccgg ccaacgccgc
     2341 ccggatcgtg gcccaggtcg gcggcggcct ggacgccgcg gccggcgagg gcctggtgca
     2401 ccgggacgtc aagccggcca acgtgttcgt cgtggcccgc gacgggcacc cgcacgccta
     2461 cctcggcgac ttcgggctga gcaaggccac cgactcgcag agcgggctga cggccaccgg
     2521 caagttcctg ggcaccgtgg cctacgccgc ccccgagcag gcccagggcg agccggtcgg
     2581 ccccgccacc gacgtctacc agctgggcgg cctgctctac cgggcgctgt cgggcagcca
     2641 gccgttcccg cgcccgcgcc agatcgcgac cgtcatggcc cacatcaagg acccgccacc
     2701 caggccctcg gaggccgagc cggcgtgtcc gaaggcgttc gaccgggtgg tggccacggc
     2761 gatggccaag aagccggggg atcgctaccc cacggcatcc gccctgggcg aggccgcgct
     2821 ggcggcggca gagggcgcgg gcccgccacc cggatggccc gaggcgggta cgttcgatcc
     2881 agttcccgac tgaggagaga catgcccta
//
LOCUS       DASUPW010000257        10201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1859902, whole genome shotgun sequence.
ACCESSION   DASUPW010000257 DASUPW010000000
VERSION     DASUPW010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 10201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10201
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1859902"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VFY44_05815"
     CDS             <1..362
                     /locus_tag="VFY44_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX5909990.1"
                     /translation="LVATARALPALRGTGVQVDALVGSAGDPGERYAGGDLDPEPLNV
                     LRTEAEAGGSWEGAGGRGRWDAVAPPGPERDSFGCGDSFAAGLTYGLGAGRDLAAAVA
                     EGARAGADCATRSGPYG"
     gene            complement(359..700)
                     /locus_tag="VFY44_05820"
     CDS             complement(359..700)
                     /locus_tag="VFY44_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5909991.1"
                     /translation="MTPLSDLQEEISPAKARELHEAGEVQLVDVREQDEWDAGRIEGA
                     RHVPVAQLSDQAESLERDKPVVFYCLSGSRSAMATQAFNASGFDAHNMTGGIAAWNAD
                     GLPMEGEVAQH"
     gene            complement(711..1088)
                     /locus_tag="VFY44_05825"
     CDS             complement(711..1088)
                     /locus_tag="VFY44_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909992.1"
                     /translation="MDDETLSQEEKDEKAEQEVKEIEDNPPENLEDWPDGDAKYKTLG
                     GPDGNTGYEEGPTANLGEADVRHHEDGSVTVGGEEVDNPDDYKGDPIPGGPTDPNAAD
                     PEGLKNDDGEPESDAGKSESDDE"
     gene            1161..1535
                     /locus_tag="VFY44_05830"
     CDS             1161..1535
                     /locus_tag="VFY44_05830"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5909993.1"
                     /translation="MTERVESGSPYEARWGFCRALRTGDRIEVAGTAPIWPDGSCDPD
                     PGVQARRCLEIIEQALGELGAGLEHVVRTRMFISDRAHQDAVGEAHGAVFGDHRPVAT
                     MVVCDLLDPRWAVEIEAEAVLS"
     gene            complement(1765..3183)
                     /locus_tag="VFY44_05835"
     CDS             complement(1765..3183)
                     /locus_tag="VFY44_05835"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX5909994.1"
                     /translation="MQLPLASFLQDPDFINVLYIVAIILFILGMRQVTGPTTARRGNA
                     TAAVGMAVAVTATLLQEQVGDYGLIALGVVIGTVIGVPAAQRVKMTAMPQMVALFNGV
                     GGGAVALISWAEYRHYLEIGAGNPELDTLIPILFAAIIGSVSFWGSNIAFGKLQDLIP
                     QKPIAVPGQQVVNLVLLVLAIGASVLIASGEESEGLFILILVAAGILGNMVVLPIGGA
                     DMPVVISLLNALTGLSAAAAGVALDNTALIVGGIIVGASGTILTNLMAEAMNRSIPNI
                     VAGGFGGGAAASGGGDAEEKPHKSTDAMSVAMALTQADAVVVAPGYGLAVAQAQHTVR
                     ELADELEKRGTEVNYAIHPVAGRMPGHMNVLLAEADVPYDALKEMDEINPEFGTTDVT
                     LVIGANDVTNPAAREDESSPIYGMPILDVDKSQSVVVFKRSMSPGFAGIDNPLFYNEN
                     TEMLFGDAKASVQEVLSAVKDL"
     gene            complement(3188..3484)
                     /locus_tag="VFY44_05840"
     CDS             complement(3188..3484)
                     /locus_tag="VFY44_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018207317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX5909995.1"
                     /translation="MDLTTELLILVLAAFVGFEVISKVPNSLHTPLMSATNAIHGIVV
                     LGGILVIGSADGVLNKTILVIAIAFGTINVVGGFLVTDRMLEMFKARPKPSEKE"
     gene            complement(3487..4614)
                     /locus_tag="VFY44_05845"
     CDS             complement(3487..4614)
                     /locus_tag="VFY44_05845"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEX5909996.1"
                     /translation="MKLSVPKESSAGERRVATVPEVVKKLSTSGVDVVVEPGAGAESH
                     VPDSAYEDAGASTGDGFSGDVVAKVAPPSPEEISKLAKGSVLIGFLAPLTAGDTAKAL
                     ADAGVTSFAMEAIPRITRAQSMDALSSQATVGGYRAALITAEELPRFLPMLTTAAGTI
                     RPAKVLVLGAGVAGLQAIATSRRLGAVVSAFDVRSAVEEQIQSLGAKFVKLDIGLEDA
                     EDAGGYARALTDEEQQRQREALADVIAQHDGVISTAAVPGRKAPLLIIEDAVKRMKPG
                     SVIVDLAAETGGNCELTKAGETVIEHDVKIIGAVNLPATMPDHASQLYAKNVQNLVEL
                     MTDDEGGLTLNFEDEIIKGACITHEGQIVHEGAKKAAGGGS"
     gene            complement(4657..5376)
                     /locus_tag="VFY44_05850"
     CDS             complement(4657..5376)
                     /locus_tag="VFY44_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5909997.1"
                     /translation="MINLIRGEAIKMRSTRTAIGFTIAGVGLTVLFVLVSTLAGEPST
                     VIEKRETLSVAPLFILFVVFGVVGATGEYRHRTVAPAVLIAPDRLRLLLARTLAYAVT
                     AAGVALAMLLTSFVLGLPLMAGNEGGALDFGDYAALVGGTVLGAALGSALGVGFGALV
                     GNQVAGVVSVLVYLFVVDSLIGVAKSDLIPYTIGTAAPALGGLEIDEAFGFGGSLAVL
                     AAWTLGFIGLGMWRESRREVT"
     gene            complement(5373..6275)
                     /locus_tag="VFY44_05855"
     CDS             complement(5373..6275)
                     /locus_tag="VFY44_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5909998.1"
                     /translation="MPGAAIEVEGLTKRYGGQTVVDDVSFSVRAGAVTGFLGPNGAGK
                     TTTLRCLLGLARATEGKATILGSAYRDLDDPARTVGANLEVSGAHPGRSGRQHLRSLA
                     AMAGIPDSRVNEVLELTEMTEAADRRYGKYSMGMRQRIGLAAALLGEPEILVLDEPAN
                     GLDPAGIRWLRDMLRATAAQGRTVLVSSHLLAEMAQTVNDVVVIHKGRVVKQGPVGEL
                     AGAARGATIVRSPQAGALAAALRSAGAEVTEFEDGALNVDGMDAAAVGDAALAAGLAV
                     HQLYEAQASLEDVFFQLTDGEAAT"
     gene            complement(6289..6759)
                     /locus_tag="VFY44_05860"
     CDS             complement(6289..6759)
                     /locus_tag="VFY44_05860"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5909999.1"
                     /translation="MRPIRAADKDALSDALAHLSPASVHARFLGPKSRFSSSELRYLT
                     EVDGHDHVALVAELAEAPGTIAGVGRWVRLPDAPDTAEFAIVVGDPLQGKGLGSVLAD
                     DLVALAKAHGISRFTATALSENAAIVRIMQRLSDHLERHHGGHGVSEISFELAA"
     gene            complement(6911..7882)
                     /locus_tag="VFY44_05865"
     CDS             complement(6911..7882)
                     /locus_tag="VFY44_05865"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5910000.1"
                     /translation="MRITLALFAALALALTASACGDDDSDSSGGGDSGGGSAELIKKD
                     PANAKKKTIIVGSKNFDEQYILGEIYAQALQAAGFKVEKKLNLGSEQIAYKALKSGQV
                     DMYPEYTGTSLTSFFKVKTDDVPKDPDAAYEEAKKDYAKEGIVAMPRTSFENTYRIAT
                     TKEKQKSILKGAKTVSEVAKLPNASKITIAGFPECEQRTDCLLGLKNLYKWTPKFVSD
                     EGKYQPIDKDRSDTGFVFSTDGELTLGKYAIIEDDKGLFPPYNISLGVRDSTYKELGK
                     EGQDVILKVQKPLTEKAMQELNSRVSLDKQEPEAVAAAYLKESGFTK"
     gene            complement(7891..8658)
                     /locus_tag="VFY44_05870"
     CDS             complement(7891..8658)
                     /locus_tag="VFY44_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5910001.1"
                     /translation="MSTTIALLGDFGDAIKFIFQERESVGGGVQVGGIPELWGFTKTH
                     LLVSLAAMVVASAISIPIGLYLGHKGKGEFLAISVSNVGRAVPAVVLVFFLVAYLGNG
                     FLNITVVLVALAIPPILTNTYVGTAQVDRDIVDAARGMGMRDGQIVRRVRLPLAMPTI
                     FAGIRTSAVSVLATATIGPYADVKTLGWPILTPQTYGFSGQLGAALLVAFITLIADAG
                     IGYLQHVVTPKGIKLAGEQGPVRRRLALPTRRREAAT"
     gene            complement(8655..9374)
                     /locus_tag="VFY44_05875"
     CDS             complement(8655..9374)
                     /locus_tag="VFY44_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5910002.1"
                     /translation="MISADLFGLFAQLQTRNRQGQANCVSEDGFCPDWIADNWQRYQD
                     PFWEHVFLTLVSVAAGFLIAFTLALIAHRRQWLIGPITGFTGALYTIPSVAFFFLLQP
                     FTGLGKTTAIVALTCYTLLIIFRNVTTGLQAVPDEAKDAARGMGLTENQLLFRVELPL
                     AAPEILAGLRIATTTTVGLAALAFLAGAGGLGAELDQQPYFKSNVVVAGGLCVLLAAV
                     LDLLILGVQRLTTPWQRAAAT"
     gene            complement(9371..>10201)
                     /locus_tag="VFY44_05880"
     CDS             complement(9371..>10201)
                     /locus_tag="VFY44_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5910003.1"
                     /translation="GWDKQRISARVDELLQLVSLDPDETRDRYPAQLSGGQRQRVGVA
                     RALAVDPPLMLMDEPFGAIDPINRERLQNEFLRLQKEIRKTIVFVTHDIDEAIKMGDK
                     IAVLQKGGKLAQFASPAELLMDPASKFVEDFVGADRALKRLALQRVRDIDLWKVAQVR
                     VGEVSSDARHKVEDADLKSHVLVVDEHGRPQGWLAVSDLGSERVPAGAQGGATVEMDD
                     ILRDALSDLLTAEARYGAVVDDKGVVQGALSIEVISRFLHEAPTDSRSGADLVEDPAA
                     "
BASE COUNT         1427 a         3536 c         3631 g         1607 t
ORIGIN      
        1 tgctggtggc caccgcgcgc gccctgcccg cgctgcgcgg caccggcgtg caggtggacg
       61 cgctggtggg cagtgccggc gaccccggcg agcggtacgc cggcggcgac ctggaccccg
      121 agcccctgaa cgtgctgcgc accgaggccg aggccggggg cagctgggag ggcgccggcg
      181 gccggggccg ctgggacgcg gtcgccccgc ccggccccga gcgcgacagc ttcgggtgcg
      241 gcgactcgtt cgccgccggg ctgacctacg ggctcggcgc gggccgggac ctggccgccg
      301 cggtcgccga gggcgcgcgg gcgggggcgg actgcgccac ccgctcgggc ccgtacggct
      361 agtgctgggc cacctcgccc tccatcggta ggccgtcggc gttccaggcg gcgatgccgc
      421 cggtcatatt gtgggcgtcg aaccccgagg cgttgaacgc ctgggtggcc atggccgagc
      481 ggctgcccga caggcagtag aagaccaccg gcttgtcgcg ctccaggctc tcggcctgat
      541 cgctgagctg ggccacgggc acgtgccggg cgccctcgat gcgcccggca tcccattcgt
      601 cctgctcgcg cacgtcgacg agctgcacct cgccggcctc gtgcagctca cgggccttgg
      661 cggggctgat ttcctcttgg aggtcggaga gcggcgtcat gccgccaacc ttattcgtcg
      721 tcgctctcgc tcttgccggc gtcgctctcg ggctcgccgt cgtcgttctt gagcccctcg
      781 gggtcggccg cgttggggtc cgtgggcccg ccggggatcg ggtcgccctt gtagtcgtcg
      841 gggttgtcga cctcctcgcc gccgacggtc acgctgccgt cctcgtggtg gcgaacgtcg
      901 gcctcgccca ggttggccgt ggggccttcc tcgtagccgg tgttgccgtc ggggccgccc
      961 agcgtcttgt acttggcgtc gccgtcgggc cagtcctcga ggttctccgg cgggttgtcc
     1021 tcgatttcct tgacctcctg ctcggccttc tcgtccttct cttcctgcga cagcgtctcg
     1081 tcgtccatcg gttcctcccc tttcgagatc ttgcggacgc ataccccggg cacgcgaccc
     1141 tcaatcggta ccgtcggccc atgaccgagc gcgtggagtc cggatcgccc tacgaggccc
     1201 gctggggctt ctgccgcgcc ctgcgcaccg gggaccggat cgaggtggcg ggcacggcgc
     1261 cgatctggcc cgacggctcg tgcgaccccg atcccggcgt ccaggcccgg cgctgcctgg
     1321 agatcatcga gcaggccctg ggcgagctcg gggccggcct ggagcacgtc gtgcgcacgc
     1381 gcatgttcat ctccgaccgc gcccaccagg acgcggtggg ggaggcacac ggagcggtgt
     1441 tcggcgacca tcggccggtg gccacgatgg tggtgtgcga cctgctcgac ccgcgctggg
     1501 cggtcgagat cgaggccgag gcggttctct cgtaggcgtc cgcgcaggct cggcgcgtac
     1561 ggatggctgg gtaggggacg gggagggaca ccacacgtcc cgatggacga ttcgcctgcg
     1621 ctgggacgtc aggtgttccc gcaagggacg tctcacgtcc cggcgggcga ttcgcctgcg
     1681 ctgggacgtc agatgtcccg ccctccgcct acccggccaa ggcgtgcgcc gagcccgcag
     1741 gggcgggccc acggcggacg ccgcctacag gtccttgacg gcgctgagca cttcctgcac
     1801 ggaagccttc gcgtcgccga acagcatctc ggtgttctcg ttgtagaaca gcgggttgtc
     1861 gatgccggcg aagcccgggc tcatcgagcg cttgaacacg accacgctct gtgacttgtc
     1921 cacgtccagg atcggcatgc cgtagatcgg cgagctctcg tcctcgcgcg cggccgggtt
     1981 ggtcacgtcg ttggcgccga tcaccagcgt cacgtccgtg gtgccgaact ccgggttgat
     2041 ctcgtccatc tccttgagcg cgtcgtaggg cacgtcggcc tcggccagca gcacgttcat
     2101 gtggccgggc atgcggcccg cgacgggatg gatggcgtag ttgacctcgg tgccgcgctt
     2161 ctccagctcg tcggccagct cgcgcaccgt gtgctgggcc tgggccacgg ccaggccgta
     2221 gccgggcgcc accacgaccg cgtcggcctg ggtcagggcc atcgccaccg acatcgcgtc
     2281 ggtggacttg tgcggcttct cctccgcgtc gccgccgccg gaggccgccg cgccgccacc
     2341 gaagccgccg gccacgatgt tggggatcga ccggttcatg gcctcggcca tcaggttggt
     2401 caggatcgtg cccgaggcgc cgacgatgat gccgccgacg atcagcgcgg tgttgtccag
     2461 cgcgacgccg gccgcggcgg ccgacaggcc ggtcagggcg ttcagcagcg agatgaccac
     2521 cggcatgtcc gcgccaccga tgggcagcac gaccatgttg cccaggatgc ccgcggcgac
     2581 caggatcagg atgaacaggc cctcggactc ctcgccgctg gcgatcagca ccgaggcgcc
     2641 gatggccagc accagcagca ccaggttcac gacctgctgg ccgggaaccg cgatcggctt
     2701 ctgggggatc aggtcctgca gcttgccgaa ggcgatgttc gaaccccaga aggagaccga
     2761 gccgatgatg gcggcgaaca ggatcgggat cagcgtgtcc agctcgggat tgccggcgcc
     2821 gatctcgagg tagtggcggt actcggccca cgagatcagg gccacggcgc cgccgccgac
     2881 gccgttgaac agcgccacca tctgcggcat ggcggtcatc ttcacccgct gggccgcggg
     2941 cacgccgatg accgtgccga tcacgacgcc cagcgcgatc aggccgtagt cgcccacctg
     3001 ctcctgcagc agcgtggccg tgaccgcgac ggccatgccc acggcggcgg tggcgttgcc
     3061 cctgcgcgca gtcgtcggtc ccgtgacctg gcgcatgccc aggatgaaca ggatgatcgc
     3121 gacgatgtag aggacgttga tgaagtccgg gtcctgcagg aatgaggcga gcgggagttg
     3181 catcgagcta ctccttctct gaaggcttcg ggcgcgcctt gaacatctcg agcatccggt
     3241 cggtgaccag gaagccgccg accacgttga tcgtgccgaa ggcgatcgcg atcaccagga
     3301 tcgtcttgtt caggacgccg tcggcggagc cgatgaccag gatcccgccc agcaccacga
     3361 tgccgtggat cgcgttggtg gcgctcatga gcggcgtgtg cagcgagttc ggcaccttcg
     3421 agatgacctc gaagccgacg aacgcggcca gcaccaggat gagcagttcg gtcgtgagat
     3481 ccatggctag gagccccctc cggcggcctt cttggcgccc tcgtggacga tctggccctc
     3541 gtgggtgatg caggcgccct tgatgatctc gtcctcgaag ttcagcgtga gcccaccctc
     3601 gtcgtcggtc atcagttcga ccaggttctg cacgttcttg gcgtagagct gcgatgcgtg
     3661 gtcgggcatc gtggcgggca ggttcaccgc gccgatgatc ttcacgtcgt gctcgatcac
     3721 ggtctcgccg gccttggtca gctcgcagtt gccgccggtc tcggccgcca ggtccacgat
     3781 caccgagccg ggcttcatgc gcttgacggc gtcctcgatg atcagcagcg gcgccttgcg
     3841 gcccgggacg gcggcggtgg agatcacgcc gtcgtgctgg gcgatcacgt cggccagcgc
     3901 ctcgcgctgg cgctgctgct cctcgtcggt cagggcgcgg gcgtagccgc cggcgtcctc
     3961 ggcgtcctcg aggccgatgt ccagcttcac gaacttggcg cccagcgact ggatctgctc
     4021 ctcgaccgcg gagcgcacgt cgaaggcgga gaccacggca cccaggcggc gcgaggtggc
     4081 gatggcctgc aggccggcca cgccggcgcc cagcaccagg accttggccg ggcggatggt
     4141 gccggcggcg gtggtcagca tgggcaggaa gcgggggagc tcctcggcgg tgatcagcgc
     4201 ggcccggtag ccaccgacgg tggcctgcga ggacagggcg tccatcgact gggcgcgcgt
     4261 gatgcgcggg atggcctcca tggcgaagct ggtcacgccc gcgtcggcca gggccttcgc
     4321 ggtgtcaccg gcggtcagcg gcgccaggaa gccgatcagg acgctgccct tggccagctt
     4381 ggagatctcc tcggggctgg gcggggcgac cttggccacc acgtcgcccg agaagccgtc
     4441 gcccgtgctg gccccggcat cctcgtaggc ggagtcgggc acgtgggact ctgccccggc
     4501 tccgggctcg acaacgacat ccaccccgga ggtggacagc ttcttgacga cctccggcac
     4561 ggtggcgacc ctgcgctcgc ctgcggagga ctccttgggg acagaaagct tcatcggcgg
     4621 ccgcggaagc ctaatgaagc ggcgggcgga aggcgttcag gtcacctcgc ggcgcgactc
     4681 gcgccacatc ccgagcccta tgaagcccag cgtccatgcg gccagcacgg ccagcgatcc
     4741 cccgaatccg aaggcctcgt cgatctccag gccgcccagc gcgggcgccg cggtgccgat
     4801 cgtgtacggg atcaggtccg acttggccac gccgatcagg ctgtccacca cgaacaggta
     4861 gaccagcacg ctgaccacgc ccgccacctg gttgcccacc agcgcgccga agcccacgcc
     4921 gagcgcgctg cccagggccg cgcccagcac cgtgccgccc accagggcgg cgtagtcgcc
     4981 gaagtccagc gccccgccct cgttgccggc catcagcggt aggcccagca cgaacgaggt
     5041 cagcagcatg gccagcgcca cgccggcggc ggtcacggcg taggccagcg tccgcgccag
     5101 cagcaggcgc agccggtccg gggcgatcag cacggcgggg gcgaccgtgc ggtggcggta
     5161 ctccccggtg gcgccgacca cgccgaagac cacgaacagg atgaacagcg gcgccacgct
     5221 gagcgtctcg cgcttctcga tcaccgtcga cggctccccg gccagcgtgg acaccagcac
     5281 gaacagcacg gtcaggccga ccccggcgat ggtgaagccg atggccgtgc gcgtgctgcg
     5341 catcttgatc gcctcgccgc ggatcaggtt gatcacgtgg cggcctcgcc gtcggtcagc
     5401 tggaagaaga cgtcctcgag cgaggcctgt gcctcgtaga gctggtgcac ggccagcccc
     5461 gcggccagcg ccgcatcgcc caccgccgcc gcgtccatgc cgtccacgtt cagcgcgccg
     5521 tcctcgaact cggtgacctc ggctccggcg gagcgcaggg ccgctgccag cgccccggcc
     5581 tggggtgagc gcacgatcgt ggccccccgc gcggccccgg ccagctcgcc gaccggtccc
     5641 tgcttgacca cgcgcccctt gtggatgacc accacgtcgt tcacggtctg cgccatctcg
     5701 gccagcaggt ggctggacac gagcaccgtc cgcccctgcg cggcggtggc ccgcagcatg
     5761 tcgcgcagcc accggatccc ggccgggtcc aggccgttcg ccggctcgtc cagcaccagg
     5821 atctccggct cgcccagcag cgcggcggcc agaccgatgc gctggcgcat gcccatcgag
     5881 tacttgccgt agcggcggtc ggccgcctcg gtcatctcgg tgagctccag cacctcgttc
     5941 acgcgcgagt ccgggatccc ggccatcgcg gcaagcgagc gcaggtgctg gcggccggag
     6001 cggcccgggt gggcgccgga cacctccagg ttggccccga cggtgcgcgc cgggtcgtcc
     6061 aggtcgcggt aggccgagcc caggatggtc gccttgccct cggtggcccg cgccaggccg
     6121 agcaggcagc gcagggtggt ggtcttgccg gccccgttgg ggcccaggaa accggtgacc
     6181 gcgcccgcgc ggacggagaa ggacacgtcg tcgaccaccg tctggccgcc gtaccgcttg
     6241 gtcagtccct cgacctcgat tgcggcgccg ggcatgcgcc caagctatct aggcggcgag
     6301 ctcgaacgag atctcggaca cgccgtggcc gccgtgatgg cgctccaggt ggtcggagag
     6361 gcgctgcatg atccggacga tcgcggcgtt ctcgctcagc gccgtggcgg tgaagcggga
     6421 gatgccgtgg gccttggcca gggcgaccag gtcgtcggcc aggacggagc ccaggccctt
     6481 gccctggagc gggtcgccga ccacgatggc gaactcggcg gtgtcgggcg cgtcgggcag
     6541 gcgcacccag cgcccgaccc cggcgatggt cccgggcgcc tctgccagct cggccaccag
     6601 cgcgacgtgg tcgtggccgt ccacctcggt caggtaacgc agctcggagg acgagaagcg
     6661 cgacttgggg cccaggaagc gggcgtggac cgaggcgggg gagaggtgtg ccaacgcgtc
     6721 ggacagggcg tccttgtcgg cggcacggat cggacgcacg gtgacgatcg ttccgtcttt
     6781 cagcgtgcgt tccatacctc tatgttcggc acgcggcaac ctttggactg tgggtgtggt
     6841 cacacctgca acgacgaggg gcgcggccct cgcggaccgc gcccctcgga actgcgtggt
     6901 gaagcgggag ctacttggtg aagccggact ccttcaggta ggcggcggcc acggcctccg
     6961 gctcctgctt gtccaggctg acacgggagt tcagctcctg catcgccttc tcggtcaggg
     7021 gcttctggac cttcaggatc acgtcctggc cctccttgcc gagctccttg taggtggagt
     7081 cccggacgcc cagcgagatg ttgtagggcg ggaacaggcc cttgtcgtcc tcgatgatcg
     7141 cgtacttgcc cagggtgagc tctccgtcgg tggagaacac gaaccccgtg tccgagcgat
     7201 ccttgtcgat cggctggtac ttgccctcat cggacacgaa cttcggtgtc cacttgtaga
     7261 ggttcttcag gcccagcagg cagtccgtgc gctgctcgca ctccgggaac ccggcgatcg
     7321 tgatcttgct ggcgttcggg agcttggcca cctcggagac ggtcttggcg cccttgagga
     7381 tgctcttctg cttctccttg gtcgtggcga tccggtacgt gttctcgaag gacgtgcgcg
     7441 gcatggccac gatgccctcc ttggcgtagt ccttcttggc ctcctcgtac gccgcgtccg
     7501 ggtccttcgg gacgtcgtcg gtcttcacct tgaagaacga cgtcagcgag gtgccggtgt
     7561 actccgggta catgtccacc tggcccgact tcagcgcctt gtaggcgatc tgctcggagc
     7621 ccaggttgag cttcttctcc accttgaacc cggcggcctg gagtgcctgc gcgtagatct
     7681 cgccgaggat gtactgctcg tcgaagttct tcgagccgac gatgatcgtc ttcttcttcg
     7741 cgttggccgg gtccttcttg atcagctcgg ccgagccgcc gcccgagtcc ccgccgcccg
     7801 agctgtcgga gtcgtcgtct ccgcaggcag atgccgtcag cgcgagcgcc aacgcggcga
     7861 acagggccag ggtgatgcgc aatatacgag tcacgttgct gcttccctcc ttcgtgtggg
     7921 cagggcgagt cggcgccgta cgggcccctg ctctccggcc agcttgatcc ccttcggggt
     7981 tacgacgtgt tgcagatagc cgattccggc gtctgcgata agcgtgatga acgcgaccag
     8041 cagcgccgcg cccagctgcc ccgagaagcc atacgtctgc ggggtcagga tgggccagcc
     8101 cagcgtcttc acgtcggcgt aggggccgat cgtggcggtg gccaggacgc tgacggccga
     8161 ggtccggatg ccggcgaaga tcgtgggcat ggccagcggc aggcgcaccc ggcgcacgat
     8221 ctggccgtcg cgcatcccca tgccacgggc ggcgtccacg atgtcgcggt ccacctgggc
     8281 ggtgcccacg tacgtgttgg tcaggatcgg cgggatcgcc agggccacca gcacgacggt
     8341 gatgttcagg aacccgttgc ccagataggc gaccaggaag aagaccagca ccacggccgg
     8401 gacggcacgg ccgacattgg acacagagat ggccaggaac tcgcccttgc ccttgtggcc
     8461 caggtagagg ccgatgggga tcgagatggc ggaggccacg accatggcgg ccaggctgac
     8521 cagcaggtgg gtcttggtga agccccacag ctcggggatg ccgcccacct ggacgccgcc
     8581 gcccaccgac tcgcgctcct ggaagatgaa cttgatcgcg tcgccgaagt cgcccagcag
     8641 ggcgatcgtg gtgctcatgt cgccgccgcc cgctgccacg gggtggtcag gcgctgcacg
     8701 cccaggatca gcaggtcgag gaccgcggcg agcaacacgc acaggccccc ggcgaccacg
     8761 acgttggact tgaagtaggg ctgctggtcc agttcggcgc ccaggccgcc ggcgccggcc
     8821 aggaacgcca gcgcggccag cccgaccgtg gtggtcgtgg cgatgcgcag gccggccagg
     8881 atctcgggcg cggccagggg caactcgacc cggaacagca gttggttctc ggtcaggccc
     8941 atgccgcggg cggcgtcctt ggcctcatcg ggcaccgcct gcaggcccgt ggtcacgttc
     9001 cggaagatga tcagcagcgt gtaacacgtg agcgccacga tcgcggtcgt cttgcccagc
     9061 ccggtgaacg gctgcagcag gaagaagaag gcgacgctgg ggatcgtgta gagggcgccg
     9121 gtgaagccgg tgatgggccc gatcagccac tgtcgccggt gcgcgatcag cgccagcgtg
     9181 aaggcgatca ggaagcccgc ggccaccgag accagggtca ggaacacgtg ctcccagaag
     9241 gggtcctggt agcgctgcca gttgtcggcg atccagtcgg ggcagaaccc gtcctcggag
     9301 acgcagttgg cctgcccctg ccggttgcgc gtctggagct gggcgaagag cccgaacagg
     9361 tccgcggaga tcacgcggcc gggtcctcca cgaggtcggc gccgctgcgt gagtcggtgg
     9421 gggcctcgtg gaggaaacgc gagatgacct cgatggacag cgccccctgc acgaccccct
     9481 tgtcgtcgac gacggcgccg tagcgcgcct cggcggtcag gaggtcggag agcgcgtcgc
     9541 ggaggatgtc gtccatctcc acggtggcgc cgccctgggc gccggcgggc acgcgctcgc
     9601 tgcccaggtc gctcaccgcg agccagccct gggggcggcc gtgctcgtcg accaccagca
     9661 catggctctt gaggtcggcg tcctcgacct tgtggcgcgc gtcggagctg acctcgccga
     9721 ccctgacctg ggcgaccttc cacaggtcga tgtcgcggac ccgctggagt gccaggcgct
     9781 tgagcgcgcg gtccgcgccc acgaagtcct cgacgaactt ggaggcgggg tccatcagca
     9841 gctcggccgg ggacgcgaac tgggccagct tgccgccctt ctgcaggacg gcgatcttgt
     9901 cgcccatctt gatcgcctcg tcgatgtcgt gggtcacgaa gacgatcgtc ttgcggatct
     9961 ccttctggag gcgcaggaac tcgttctgca ggcgctcgcg gttgatcggg tcgatagcac
    10021 cgaagggctc gtccatgagc atcagcggtg ggtcgacggc cagggcgcgg gcgacgccga
    10081 cgcgctggcg ctggccgccg gacagctggg cggggtagcg gtcgcgggtc tcgtccgggt
    10141 ccagcgacac cagctggagc agctcgtcca cccgggcgga gatgcgctgc ttgtcccagc
    10201 c
//
LOCUS       DASUPW010000258         3765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_751174, whole genome shotgun sequence.
ACCESSION   DASUPW010000258 DASUPW010000000
VERSION     DASUPW010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751174"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..3010)
                     /locus_tag="VFY44_05885"
     CDS             complement(95..3010)
                     /locus_tag="VFY44_05885"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX5910004.1"
                     /translation="MALTLVTGPANAAKAGAVLGPLRARLDEQPVLVVPAFQDVEHSR
                     RELAETGATFGAQVVRFSHLSRMIARRTGYEARVASELQQQLVAEDAVRRAGFTVLAD
                     SAARPGFARAALRFIAELERSMVEPPRLIQALRSWAGDGPRAAYAEEIGELYRRYREG
                     LDAAGLVDDDLFAWGALAALRARPSAWAGTPVFVYGFDDFTELEFRTIEELAVYAEAD
                     VVVSLPFEPGREAFKATARVREQLREIAAEEKQLDAVSDHYDKGSRSALHALERTLFE
                     PGGATADAGQAVTVHAAGGERAELELCGAEVVALLRDGTPPGDVAVVFRDPPRYASLV
                     EQVFGAYGIPYSMDRAVPFAHTGVGRGLLALLRCALLEGTADDLLAYLRTPGLLREPG
                     LADRLEADVRRRGERTADGARALWEERPGRWPLDVIDELRDAPDAAALLDRLAHRLVR
                     LFSAPYRREAARMQGAELDDPRAFRAGHAALTEMRAVVKADPDVNLDARHVHETLSEL
                     PVRVGEEAQTDRVQVASPLDVRARRFDAVFVCGLQEKEFPQGGSPDAFLPDTDRRELA
                     RASGLLLPLREDRLERERYLFYVCASRAERRLVLSARYCDEEGNPDAPSFFVDDARAV
                     FADMAERTRSLADVTWPLDQAPTEAEWERTVAHTGPRRQDTVVGPLALPAALEPLETK
                     DAVSAGALERFAGCPVSWLVDDVLKPEKLEPDPEAMVRGSYAHKVLEVTFKRLREQTG
                     DRRVTPANLGTAERILLEALEAERGEFRLSPNQTRVRAAVRRLEFDLLRYLRHEAGRD
                     GSFEPEYLELRFGFEDSDHPAVELADGTRVRGLIDRIDTWDGWALVRDYKSGRTAQYK
                     VADWEKENHFQAALYMLVAQRALGLKPAGGVYVPLAGTERRPRGMVAAELVGQLGSDF
                     YDNDVVPEADFAERTDWAQRSIATTATRMRGGDLISCPDSCAWRGGCSHPSICRVET"
     gene            complement(3055..3684)
                     /locus_tag="VFY44_05890"
     CDS             complement(3055..3684)
                     /locus_tag="VFY44_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910005.1"
                     /translation="MSLFCRHNRFVADCPICSKGTVLESGSSSSRRSSSGGTRKPAKA
                     KPAPAAHGPSVSSPAYEDADGVAYVVRLERVPGGVRLAEWQGSTLRRRAPVLGAADLV
                     RLAEAAGQVLADGDAAALSAATASEQAEGPAQGPGVSKGRAGDFKEELRVEALEDGRV
                     RVGRWVLRPNQGWQLQEAPPMLPAKRYAEAVAAAVRKGLLPQASEAAVD"
BASE COUNT          455 a         1479 c         1375 g          456 t
ORIGIN      
        1 gaggtcttgc cgctgcccgc cccggcgctg accaggaccg acccgtcgcg gcgcccgatg
       61 gcccgcgcct gctcctcggt gaagtcgggg cgggtcacgt ctccacccgg cagatcgacg
      121 ggtgcgagca gccgccgcgc cacgcgcagc tgtccgggca cgagatcagg tcgccgccgc
      181 gcatgcgcgt ggccgtggtg gcgatcgagc gctgggccca gtcggtgcgc tcggcgaagt
      241 ccgcctcggg caccacgtcg ttgtcgtaga agtcgctgcc cagctgcccc accagctcgg
      301 cggcgaccat gccgcggggg cggcgctcgg tgcccgcgag cggcacgtag acgccgccgg
      361 cgggcttgag gcccagggcg cgctgggcga ccagcatgta cagcgccgcc tggaagtggt
      421 tctccttctc ccagtcggcc accttgtact gggcggtgcg cccgctcttg tagtcgcgga
      481 ccagcgccca cccgtcccag gtgtcgatgc ggtcgatcag cccgcgcacg cgcgtcccgt
      541 cggccagctc gaccgccggg tggtcggagt cctcgaaccc gaaccggagc tccaggtact
      601 cgggctcgaa cgacccgtcg cgtccggcct cgtggcgcag gtagcgcagc aggtcgaact
      661 ccagccgccg caccgccgcg cggacccggg tctggttggg cgacaggcgg aactcgccgc
      721 gctcggcctc cagcgcctcc agcaggatcc gctcggccgt gcccaggttg gcgggcgtga
      781 cccggcggtc gccggtctgc tcgcgcaggc gcttgaaggt gacctccagg accttgtgcg
      841 cgtacgagcc gcgcaccatc gcctccgggt cgggctccag cttctcgggc ttcagcacgt
      901 cgtcgaccag ccagctgacc gggcagccgg cgaaccgctc cagcgcaccg gccgagaccg
      961 cgtccttggt ctccagcggc tccagcgcgg cgggcagggc cagcggcccg accacggtgt
     1021 cctggcgccg tggcccggtg tgggccacgg tccgctccca ctccgcctcg gtgggcgcct
     1081 ggtccagggg ccacgtgacg tcggccagcg agcgggtccg ctcggccatg tccgcgaaca
     1141 cggcgcgcgc gtcgtcgacg aagaacgacg gcgcgtcggg gttgccctcc tcgtcgcagt
     1201 agcgcgcgct gagcaccagc cggcgctcgg cacgcgaggc gcagacgtag aacaggtacc
     1261 gctcgcgctc caggcggtcc tcgcgcagcg gcagcagcag gccgctggcc cgcgccagct
     1321 cgcgccggtc ggtgtcgggc aggaacgcgt ccggcgaccc gccctggggg aactccttct
     1381 cctgcaggcc gcagacgaac acggcgtcga aacggcgcgc gcgcacgtcc aggggcgagg
     1441 ccacctgcac gcggtcggtc tgcgcctcct cgcccacccg gacgggcagc tcggacagcg
     1501 tctcgtgcac gtggcgggcg tccaggttca cgtccgggtc ggccttgacc accgcccgca
     1561 tctcggtcag ggcggcgtgg cccgcgcgga aggcgcgcgg atcgtccagc tcggcgccct
     1621 gcatgcgcgc cgcctcgcgc cggtacggcg cgctgaacag gcgcaccagg cggtgggcca
     1681 ggcggtccag caacgccgcg gcgtccggcg cgtcgcgcag ctcgtcgatg acgtccagcg
     1741 gccaccggcc cggccgctcc tcccacagcg cgcgggcgcc gtcggccgtg cgctcgccgc
     1801 ggcggcgcac gtcggcctcg agccggtcgg ccaggccggg ctcgcgcagc aggccgggcg
     1861 tgcgcaggta ggccagcagg tcgtccgcgg tgccctccag cagcgcgcag cgcagcagcg
     1921 ccagcagccc gcggccgacc ccggtgtggg cgaagggcac cgcccggtcc atcgagtacg
     1981 ggatcccgta ggcgccgaac acctgctcga ccagcgacgc gtaccggggc gggtcgcgga
     2041 acaccaccgc cacgtccccg gggggcgtgc cgtcgcgcag cagcgccacc acctcggcgc
     2101 cgcacagctc cagctcggcc cgctcgccgc cggcggcgtg cacggtcacg gcctgcccgg
     2161 cgtcggccgt cgcgccgccc ggctcgaaca gcgtccgctc cagggcgtgc agggcggacc
     2221 gcgagccctt gtcgtagtga tcgctgaccg cgtccagctg cttctcctcg gccgcgatct
     2281 cccggagctg ctcccgcacc ctggccgtgg ccttgaaggc ctcgcgcccg ggctcgaacg
     2341 ggagcgagac cacgacgtcg gcctcggcgt acacggccag ctcctcgatc gtgcggaact
     2401 ccagctcggt gaagtcgtcg aagccgtaga cgaacaccgg cgtgcccgcc cacgccgacg
     2461 gccgcgcccg cagcgccgcc agcgcgcccc acgcgaacag gtcgtcgtcc accaggccgg
     2521 ccgcgtccag gccctcgcgg tagcgccggt acagctcgcc gatctcctcg gcgtaggccg
     2581 cccgcgggcc gtccccggcc cagctgcgca gggcctggat cagccgcggc ggctcgacca
     2641 tcgaccgctc cagctcggcg atgaagcgca aggcggcccg cgcgaacccc ggccgcgcgg
     2701 ccgagtcggc cagcaccgtg aagcccgcac ggcgcaccgc gtcctcggcc accagctgct
     2761 gctgcagttc ggacgccacc cgcgcctcgt agcccgtgcg ccgggcgatc atccgcgaca
     2821 ggtgggagaa gcgcaccacc tgggcgccga aggtcgcgcc ggtctcggcc agctcgcggc
     2881 ggctgtgctc gacgtcctgg aaggcgggca cgaccagcac cggctgctcg tccagccgcg
     2941 cgcgcagcgg gcccagcacc gcgcccgcct tggcggcgtt ggcgggtccg gtgacgaggg
     3001 tcagggccat gggcccgtca gttgtaggac gtggtcagga cgagatgcgg gtcgtcagtc
     3061 gaccgcggct tctgacgcct ggggcagcag gcccttgcgg accgccgccg cgaccgcctc
     3121 ggcatagcgc ttggccggca gcatcggcgg cgcctcctgc agctgccagc cctggttggg
     3181 gcgcagcacc cagcggccca cgcgcacgcg gccgtcctcc agcgcctcga cgcggagctc
     3241 ctccttgaag tcgcccgcgc gccccttgct gacgcccggg ccctgggccg ggccctcggc
     3301 ctgctcggag gccgtggcgg cggacagggc cgccgcgtcg ccgtccgcca ggacctggcc
     3361 cgcggcctcg gccaggcgca ccaggtcggc cgcgcccagc accggcgcgc gccggcgcag
     3421 ggtcgagccc tgccactcgg ccagcctgac cccgcccggg acgcgctcga ggcggaccac
     3481 gtaggccacg ccgtcggcgt cctcgtaggc cgggctggac acgctcgggc cgtgcgcggc
     3541 gggcgcgggc ttggccttgg cgggcttgcg ggtgccgccg gacgaggacc gccggctcga
     3601 gctgctcccg ctctcgagca ccgtgccctt ggagcagatc gggcagtcgg cgacgaagcg
     3661 attgtggcgg cagaacaggg acacggcctg tgaggatagg cgtcccgtgg ccaccgccgt
     3721 catctccgac ctgcacctgg gcgccgtctc gaccgcggac gtggc
//
LOCUS       DASUPW010000259         3296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1721508, whole genome shotgun sequence.
ACCESSION   DASUPW010000259 DASUPW010000000
VERSION     DASUPW010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1721508"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..420)
                     /locus_tag="VFY44_05895"
     CDS             complement(121..420)
                     /locus_tag="VFY44_05895"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEX5910006.1"
                     /translation="MSWAAILGLAGAAYLLKAAGPLLAGGREISPRADQVLALLSVPL
                     LAALVAVQTLDGGERIVIDARVPALAVAGVLVWRRAPFLVVVLAAAATAALLRLA"
     gene            complement(417..1055)
                     /locus_tag="VFY44_05900"
     CDS             complement(417..1055)
                     /locus_tag="VFY44_05900"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEX5910007.1"
                     /translation="MATGAYGVSFGVLAVAAGLDAAKACALSLLVFTGASQFAVIGVL
                     GAGGGVAAALAPALLLASRNLAYGLSMAPILRGSRAARAWQAQLVIDESTAMARAQED
                     PVAARHAFLATGISVFVLWNLGTLVGALAGEGLGDPRDLGLDAMFPAAFLALLAPQLR
                     RPGAPQAAVAGAVLAVALLPVAPAGVPVLVAAAGVVPGVLAARRAARGGGTT"
     gene            1150..2685
                     /locus_tag="VFY44_05905"
     CDS             1150..2685
                     /locus_tag="VFY44_05905"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX5910008.1"
                     /translation="MTRSLGVLLGLILLMALGVAPAQAAAPKFEPGARSAGDPILPQI
                     GNGGYDALRYDIALGVTPANRFSGAVTTMSARATQNLSELSMDFQDLAIRSVHVNGRR
                     AFFSQVDATPQLSSDPAITQPKKLVIKPPAGIRRGDRFQVRVDYGGAQPQVFTDPDYS
                     SEGWVPACYTPAGGTQVCDSNFVVGEPMGAQAWFPSNNHPTDKAAFETSITVPAGQTA
                     FGVGELRRRRPNANGSVTWIWSEDDPTATYLVTASNGVFDYTQSTLRSGRRTIPNYNA
                     IDPSATPAQKTALATTLGRTQEIMDFLADRYGPYPFDSNGALVDRAPSVGYALEVQGK
                     SHYSSLNTSQSTVAHEIAHQWFGNAVTLEHWNDIWFNEGWARLAQWDWNFADGTSPAS
                     AKQQFEANYAAATPQDWALAPAVLDNDPANLFLTFPTYTRGGMTLEGYRQIVGERRFN
                     AFARSLQRRYAYGNIGTRRFIAEAVQASGLRGAKAQLLGQYFEQWLYGTVKPTLVPAS
                     FSG"
     gene            2753..>3296
                     /locus_tag="VFY44_05910"
     CDS             2753..>3296
                     /locus_tag="VFY44_05910"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX5910009.1"
                     /translation="MGRSLKLVVACIAACLVVVPAASAETARYILPPGNFGGLPTTQN
                     SLDQLPLYDGLTPLRGNVTPADVDRFYLPEDFNPIGATRDEPTGRPGLRIAYDTYGVP
                     HIYGGTRDDLSFGAGWVTARDRGLLLSLGRGPARVAVADVPGIDAFSLVTSGKGFAPS
                     KQAEALVTKQKNLLVKEYGAK"
BASE COUNT          510 a         1280 c         1114 g          392 t
ORIGIN      
        1 cgagctgtgg ctgggcgtgc ccggcggcag cctgtggcag tccgtggcgt ggtcgctggc
       61 catcaccgcc gtgttcgcgc cgctggccgt gtggcgctac cgccagagcg ccgagcgggg
      121 ctaggcgagg cgaagcagcg ccgcggtcgc cgcggcggcg agcaccacga ccaggaaggg
      181 tgctcgccgc cacaccagga cgccggcgac ggcgagcgcg ggaacgcgcg cgtcgatgac
      241 gatccgctcc ccgccgtcca gcgtctggac ggcgaccagc gcggccagca gcggaacgct
      301 gagcagggcc aggacctgat ccgcgcgcgg gctgatctcg cgcccgccgg ccagcagcgg
      361 gccggccgcc ttgagcaggt aggcggcgcc cgcgaggccc aggatggcgg cccagctcac
      421 gtcgtccctc cgccccgcgc ggcccggcgg gcggccagca cgccggggac gaccccggcc
      481 gccgccacca ggaccggcac gccggcgggc gccaccggca gcagcgccac cgccagcacg
      541 gcgcccgcga ccgccgcctg gggcgcgccc gggcgccgca gctgcggggc cagcagcgcc
      601 aggaacgcgg cggggaacat ggcgtccagg cccaggtcgc gcggatcgcc caggccctcc
      661 cccgccagcg cgccgaccag cgttccgagg ttccacagca cgaacacgct gatgccggtg
      721 gccaggaacg catggcgggc ggccaccggg tcctcctggg cgcgggccat ggccgtcgac
      781 tcgtcgatga ccagctgcgc ctgccacgcc cgcgccgcgc gcgagccccg gaggatgggc
      841 gccatcgaca gcccgtaggc caggttccgt gaggccagca gcagcgccgg cgccagcgcc
      901 gccgccaccc cgccgcccgc accgagcaca ccgatgacgg cgaactgcga ggcgccggtg
      961 aacaccagca gcgacagggc gcaggccttg gcggcgtcca ggcccgcggc cacggcgagc
     1021 accccgaagg acacgccgta cgcccctgtg gccacaccga tggccaacgc gtcgcggttt
     1081 atcgccttcc ggttggacac cgcggccaat catggacatt cgtatcccgg aaggaggtat
     1141 gacacgcaca tgacccgatc cctcggcgtg ctcctcggac tgatcctgct gatggccctc
     1201 ggcgtcgccc cggcccaggc cgccgcgccg aagttcgagc ccggggcccg gtcggccggc
     1261 gaccccatcc tcccccagat cggcaacggc ggctacgacg cgctgcgcta cgacatcgcc
     1321 ctgggcgtca ccccggcgaa ccgcttcagc ggcgcggtca ccaccatgtc cgcccgcgcg
     1381 acccagaacc tgtccgagct gagcatggac ttccaggacc tggccatccg cagcgtgcac
     1441 gtgaacggcc gccgcgcctt cttcagccag gtggacgcca ccccgcagct gagctccgac
     1501 ccggcgatca cccagccgaa gaagctcgtc atcaagcccc ccgccggcat ccgtcgtggc
     1561 gatcggttcc aggtccgggt ggactacggc ggcgcacagc cccaggtgtt caccgacccg
     1621 gactactcca gcgagggctg ggtcccggcg tgctacacgc cggccggcgg cacgcaggtg
     1681 tgcgacagca acttcgtggt cggcgagccg atgggcgccc aggcctggtt cccgtccaac
     1741 aaccatccca ccgacaaggc cgccttcgag acctcgatca cggtcccggc cggccagacc
     1801 gcgttcggcg tcggtgagct gcgcaggcgc aggcccaacg ccaacggctc cgtgacgtgg
     1861 atctggagcg aggacgatcc gaccgccacc tacctggtga ccgcgtcgaa cggcgtgttc
     1921 gactacaccc agtcgacgct gcgctcgggc cggcggacga tccccaacta caacgccatc
     1981 gacccgtcgg ccacgcccgc ccagaagacg gcgctggcca cgaccctggg ccgcacccag
     2041 gagatcatgg acttcctggc cgaccgctac ggcccgtacc cgttcgactc caacggggcc
     2101 ctggtcgacc gcgcccccag cgtcgggtac gcgctcgagg tccagggcaa gtcccactac
     2161 tcgtcgctga acaccagcca gtcgacggtg gcccacgaga tcgcccacca gtggttcggc
     2221 aacgcggtca ccctcgagca ctggaacgac atctggttca acgagggctg ggcacgcctg
     2281 gcccagtggg actggaactt cgccgacggc accagcccgg ccagcgccaa gcagcagttc
     2341 gaggccaact acgcggccgc caccccccag gactgggccc tggccccggc cgtgctggac
     2401 aacgaccccg ccaacctgtt cctgacgttc cccacctaca cgcgcggtgg catgacgctg
     2461 gagggctacc gccagatcgt cggcgagcgg cgcttcaacg cgttcgcccg ctcgctgcag
     2521 cggcgctacg cgtacggcaa catcggcacg cgccggttca tcgccgaggc ggtccaggcc
     2581 tcgggcctgc gaggcgcgaa ggcgcagctg ctgggccagt acttcgagca gtggctctac
     2641 ggcaccgtta agcctacgtt ggtgcccgcc tcgttctctg gctgaccggc cagaaatatg
     2701 aaacagtgcc gcaccacagg gctgggaggc gctggggaga gagggagccc gtatgggtcg
     2761 cagtctgaag ctggtcgtgg cgtgcatcgc cgcatgcctc gtcgtcgtgc ccgccgcatc
     2821 ggcggagacc gcccgctaca tcctgccccc gggcaacttc ggtggcctgc ccaccacgca
     2881 gaactccctg gaccagcttc cgctgtacga cggcctgacc ccgctgcggg gcaacgtgac
     2941 cccggcggac gtggaccgct tctacctccc cgaggacttc aacccgatcg gcgccaccag
     3001 ggacgagccc accggccgcc ccgggctgcg gatcgcctac gacacctacg gcgtgcccca
     3061 catctacggc ggcacccgcg acgacctctc cttcggcgcc ggctgggtga ccgcgcgtga
     3121 ccgcgggctg ctgctgagcc tgggccgtgg cccggcccgc gtggccgtcg ccgacgtgcc
     3181 gggcatcgac gccttctcgc tggtcaccag cggcaagggc ttcgcgccca gcaagcaggc
     3241 cgaggcgctc gtgaccaagc agaagaacct gctggtcaag gagtacggcg cgaagg
//
LOCUS       DASUPW010000260         5437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1721728, whole genome shotgun sequence.
ACCESSION   DASUPW010000260 DASUPW010000000
VERSION     DASUPW010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1721728"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1683)
                     /locus_tag="VFY44_05915"
     CDS             complement(<1..1683)
                     /locus_tag="VFY44_05915"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX5910010.1"
                     /translation="MSLRQLLTYAHEDPAVDALNEAAAQSPQRVFVSSSLRPYLLAAL
                     ADLDLRRPALIVAGDDRQARDLAADLKQFLHPRPVHLYPARGVAYESHLAPPPHLTGL
                     RIAALDALRAEPDPQRGAAVVVAGAVALAEKVPDPELRPHGFAIQKGELLDLDETAER
                     LVGCGYHRVDQVEERGQFALRGDILDVYPATEERAVRCELFDVEVERLTFFSTFTQRS
                     LEEVETVAIEPAAELAPEHRELAEIAAGTEDGERPDVAEVLPVDRFGEVLSLIPEGGL
                     VAIAAEEELAPSLRDYWDDVTAAFHDADAHHLYVHPEQLEGTLAERASMSLSSISGDQ
                     PHTFRAQAADTAARSLKAAEPELEKLIRSGYRTIIAWARTGEAERAAYNLDRVKATFL
                     DGNEAPHDPGLHFASSSLREGFLSPSLKLAIIPEHRLLRRRRSDQRGGPRTGAGAMAS
                     FTDLRAGVTVVHEDHGIARFTRFETKTVGDVTRDYLELEFRDGDRVFVPSDQIHKISR
                     YVGAESGETQLSKLGGKQWEQQKLRARRAAEALAGELINLYAERKRRAGYAFP"
     gene            1775..2641
                     /locus_tag="VFY44_05920"
     CDS             1775..2641
                     /locus_tag="VFY44_05920"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5910011.1"
                     /translation="MPARILSLTALTAVAVVAMAIVWSALGGQIPGSGGSAKADPADV
                     SLEPPPQSAGTAPAAEPPGRPIPVLMYHATEAPPAGTAYPDLWVPARRFAAQMQMLRR
                     EGYNAVTMRQAAAYWAGRATVPRKPVVLTFDDGFASNARVALPVLRRMGWRGVLYLEL
                     GALHTRPPEGISESDVHRLLDAGWELGSHSMEHPDLAGLPADRLRYEVARSRTELTRR
                     FGVTVDSFCYPDGSYDRAAVAEVRRAGYATATTTAAGLAAPPHPLELRRIRVEGGDSP
                     AALSARIRAAGQ"
     gene            2654..4108
                     /locus_tag="VFY44_05925"
     CDS             2654..4108
                     /locus_tag="VFY44_05925"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX5910012.1"
                     /translation="MSPPVPPLSSVPALREALERVRYSAGALMVDLGGPIDTSTGDAP
                     RQARELPDSDRAELARLFLLGRPVAADRAAEALGPASISDLVEAGWLVEDGGDVRCPL
                     RITPDEGVLLVHDPLEVSASGPDVVLGRSSAANTLAALTQRGRVGSVLDVGTGCGIQA
                     LQAAAHADRVVATDVNPRALELTALGAALSGIDNVELREGSWFEPVAGQEFDLIVSNP
                     PFVISPENDFVYRDGSGGRDEVSQLVVTQAAGHLAPGGIAQVLVNWVEDPFSSWIAPV
                     QRWLEGAGVDALVLHHLSEQPVQYAAKWHMHLHGDPDAHGRALDRWTAHFEAEGIVTL
                     ATGAIILRRTGREPALVTGLSMESAPGGRAGRHTVRLLDAARWLDSVSDEELMATPMA
                     LVDDHRLISERVHYEGEYGEDEVRVVLSDNAGVNGQLSPAAAAVLIGLEGGSTPRDAV
                     PGLAAAIPEMHADAEHLVATTVRDLVSRGLLVRR"
     gene            complement(4080..4655)
                     /gene="pth"
                     /locus_tag="VFY44_05930"
     CDS             complement(4080..4655)
                     /gene="pth"
                     /locus_tag="VFY44_05930"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX5910013.1"
                     /translation="MAEAGGRVDWLIAGLGNPGSKYEHTRHNVGFDVIAELVRRWDLP
                     RAKRKWAGQYTEGRTGPGGPRVGVLAPLTYMNEAGNSVGPARGSLGLELDQIVAVHDE
                     IDLAFGRIESRLGGGLAGHNGLKSLKRGLGSPDFNRIRVGVGRPDTTDPEIVSAYVLG
                     RFSEPKDEVQALVEAAADEVERLIGAPAAPG"
     gene            complement(4662..5363)
                     /locus_tag="VFY44_05935"
     CDS             complement(4662..5363)
                     /locus_tag="VFY44_05935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX5910014.1"
                     /translation="MADDRPSLAVEERSERGSREARRLRRSGQVPGILYGASHPDPVS
                     LKVGAIELRRLLNEGAALFDIKIGADKSVPAILKDRQDHPVRGDVMHVDFLEVNLKEK
                     IHATVALELEGVEDAPGVKEGGVLDLATREINIEALPTDIPDLIVVDVSAMDMGDTLM
                     LESVPAPEGVEFLDDLQETVVATVIFPTVEEEPEEVEEETELVGEDGEPVAEGEGGDD
                     ASEESGEDSGDSGDE"
BASE COUNT          660 a         2005 c         2030 g          742 t
ORIGIN      
        1 ggggaacgcg tagccggcgc ggcgcttgcg ctcggcgtac aggttgatca gctcgccggc
       61 cagcgcctcg gcggcgcggc gggcgcgcag cttctgctgc tcccactgct tgccgcccag
      121 cttggacagc tgggtctcgc ccgactcggc gcccacgtag cggctgatct tgtggatctg
      181 gtcgctgggc acgaacacgc ggtcgccgtc gcggaactcg agctccaggt agtcgcgcgt
      241 gacgtcgccg acggtcttgg tctcgaaccg ggtgaagcgc gcgatgccgt ggtcctcgtg
      301 caccaccgtc acgccggcgc gcaggtcggt gaacgaggcc atcgcgcccg cgccggtgcg
      361 cgggccgccg cgctgatcgg accggcggcg gcgcagcagc cggtgctcgg ggatgatggc
      421 cagcttcagc gagggcgaca ggaagccctc ccgcagcgac gaggaggcga agtgcagccc
      481 cgggtcgtgc ggcgcctcgt tgccgtccag gaaggtggcc ttgacgcggt ccaggttgta
      541 ggcggcgcgc tcggcctcgc cggtccgggc ccaggcgatg atcgtccggt agcccgaccg
      601 gatcagcttc tccagctcgg gctcggccgc cttcagcgac cgggcggcgg tgtcggcggc
      661 ctgggcgcgg aaggtgtgcg gctggtcacc cgagatggac gacaacgaca tcgacgcccg
      721 ctcggccagc gtcccctcca gctgctcggg gtgcacgtac agatggtgcg cgtcggcatc
      781 gtggaaggcg gccgtcacgt cgtcccagta gtcgcgcagg ctgggcgcca gctcctcctc
      841 cgcggcgatc gccaccaggc cgccctcggg gatcaggctc agcacctcgc cgaagcggtc
      901 caccggcagc acctcggcca cgtcggggcg ctcgccgtcc tcggtcccgg cggcgatctc
      961 ggccagctcg cggtgctcgg gggccagctc ggcggcgggc tcgatggcca ccgtctcgac
     1021 ctcctcgagc gagcgctggg tgaaggtgga gaagaaggtc aggcgctcga cctcgacgtc
     1081 gaacagctcg caccgcaccg cgcgctcctc ggtggccggg tagacgtcga ggatgtcgcc
     1141 ccggagggcg aactggcccc gctcctcgac ctggtccacg cggtggtagc cgcagcccac
     1201 caggcgctcg gcggtctcgt ccaggtccag cagctcgccc ttctggatcg cgaagccgtg
     1261 cgggcgcagc tcggggtcgg ggaccttctc ggccagcgcc accgcgccgg ccaccaccac
     1321 cgcggcgccg cgctggggat ccggctcggc gcgcagcgcg tccagggcgg cgatgcgcag
     1381 gccggtcagg tgcggcggcg gcgccaggtg cgactcgtag gccacgccgc gggcggggta
     1441 caggtgcacg ggccgcggat gcaggaactg cttgaggtcg gcggccaggt cgcgtgcctg
     1501 gcggtcgtcg ccggcaacga tcagcgccgg gcgccggagg tccaggtcgg ccagcgccgc
     1561 cagcaggtag ggccgcaggc tcgacgacac gaacacgcgc tggggcgact gcgccgcggc
     1621 ctcgttcagg gcgtcgacgg cggggtcctc gtgggcgtat gtgagcagct ggcgcaggga
     1681 catgggcacg cagagcggcc gcccggcggt gccgcgcggc tcctcacgcc cgagtctaga
     1741 tgccgtccgg gggtgggccg atcatcgccc cggcatgccc gcgcgcatcc tgtcgctgac
     1801 ggccctgacc gccgtcgccg tggtggcgat ggcgatcgtc tggtccgcgc tgggcggcca
     1861 gatcccgggc agcggcggct ccgccaaggc cgatccggcc gacgtctccc tggagccccc
     1921 gccccagtcc gccggcaccg cccccgccgc cgaacccccc ggccggccga tcccggtcct
     1981 gatgtaccac gccaccgagg cgcccccggc gggcaccgcg tacccggacc tgtgggtccc
     2041 ggcccggcgg ttcgcggccc agatgcagat gctgcggcgc gagggctaca acgccgtcac
     2101 gatgcgccag gccgccgcgt actgggccgg gcgcgcgacg gtcccgcgca agccggtggt
     2161 cctgaccttc gacgacggct tcgccagcaa cgcgcgcgtg gcgctgccgg tgctgcggcg
     2221 gatgggctgg cgcggggtgc tctacctgga gctgggcgcg ctgcacaccc ggccgcccga
     2281 ggggatctcc gagagcgacg tgcaccggct gctggacgcc ggctgggagc tgggctcgca
     2341 ctccatggag caccccgacc tggccggcct ccccgccgac cggctgcggt acgaggtcgc
     2401 gcgctcgcgg acggagctga cccgccgctt cggggtcacc gtggactcat tctgttatcc
     2461 cgacggcagc tacgaccggg ccgcggtggc cgaggtgcgc cgcgccggct acgccaccgc
     2521 cacgaccaca gccgccggcc tggcggcccc gccgcacccg ctggagctca ggcggatccg
     2581 cgtcgagggc ggcgacagcc ccgccgcgct ctccgcccgg atccgcgccg ccggccagta
     2641 gcctgccgcc gggatgagcc ctccggttcc cccgctgtcg tccgtgcccg ccctgcgcga
     2701 ggcgctggag cgcgtgcgct actcggccgg cgcgctgatg gtggacctgg gcgggccgat
     2761 cgacaccagc accggcgacg ccccgcgcca ggcgcgcgag ctgcccgaca gcgaccgcgc
     2821 cgagctggcc cggctgttcc tgctgggccg ccccgtcgcg gccgaccgcg ccgccgaggc
     2881 gctgggcccg gcttcgatct ccgacctggt ggaggccggc tggctggtcg aggacggcgg
     2941 cgacgtgcgc tgcccgctgc ggatcacgcc cgacgagggc gtgctgctgg tgcacgatcc
     3001 gctggaggtc tcggcctccg gcccggacgt ggtgctgggt cgcagctcgg cggccaacac
     3061 gctggccgcg ctgacccagc gcgggcgggt gggatccgtg ctggacgtgg gcaccggctg
     3121 cggcatccag gcgctccagg ccgcggcgca cgccgaccgg gtggtggcga cggacgtgaa
     3181 cccgcgcgcg ctggagctga ccgcgctggg cgccgcgctg tccgggatcg acaacgtcga
     3241 gctgcgcgag ggcagctggt tcgagcccgt cgccggccag gagttcgacc tgatcgtgag
     3301 caacccgccg ttcgtgatct cgcccgagaa cgacttcgtg taccgcgacg gcagcggcgg
     3361 ccgcgacgag gtctcacagc tggtggtcac gcaggcggcg ggccacctgg ccccgggcgg
     3421 catcgcccag gtgctggtga actgggtcga ggacccgttc tcgtcgtgga tcgcgcccgt
     3481 gcagcgctgg ctggagggcg ccggcgtcga cgcgctggtg ctccaccacc tgagcgagca
     3541 gccggtccag tacgccgcca agtggcacat gcacctgcac ggcgaccccg acgcccacgg
     3601 ccgggcgctt gaccgctgga ccgcgcactt cgaggccgag ggcatcgtca cgctggccac
     3661 cggggcgatc atcctgcgcc gcaccggccg ggagcccgcg ctggtcaccg ggctctcgat
     3721 ggagtcggcg ccgggcgggc gcgccgggcg ccacacggtc cggttgctgg acgccgcgcg
     3781 ctggctggac tcggtgtccg acgaggagct gatggccacc ccgatggccc tggtggacga
     3841 ccaccgcctg atcagcgagc gcgtgcacta cgagggcgag tacggcgagg acgaggtgcg
     3901 cgtggtgctg tccgacaacg ccggcgtgaa cggccagctc agcccggcgg ccgcggcggt
     3961 gctgatcggg ctggagggcg gctcgacacc ccgggacgcg gtacccggcc tggcggcggc
     4021 catccccgag atgcacgccg acgccgagca cttggtggcc acgacggtgc gcgacctggt
     4081 cagccggggg ctgctggtgc gccgatgagg cgttcgacct cgtccgccgc ggcctcgacc
     4141 agcgcctgga cctcgtcctt gggctcgctg aagcggccca gcacgtaggc cgagacgatc
     4201 tccgggtccg tggtgtcggg ccggccgacg cccaccctga tccggttgaa gtccgggctg
     4261 cccaggccgc gcttgagcga cttcaggccg ttgtggcccg ccagcccgcc gccgaggcgc
     4321 gactcgatgc ggccgaaggc caggtcgatc tcgtcgtgca ccgccacgat ctggtccagc
     4381 tccaggccga gcgacccgcg ggcggggccg acggagttgc cggcctcgtt catgtaggtc
     4441 agcggcgcca gcacgccgac gcgcggcccg ccggggccgg tgcggccctc ggtgtactga
     4501 ccggcccact tgcgcttggc gcgcggcagg tcccagcgcc ggaccagctc cgcgatcacg
     4561 tcgaagccga cgttgtggcg ggtgtgctcg tacttcgagc ccgggttgcc caggccggcg
     4621 atcagccagt cgacgcggcc gccggcctct gccatacggc gctactcgtc gccggagtcg
     4681 ccggagtcct cgccggactc ctccgaggcg tcgtcgccac cctcgccctc ggccacgggc
     4741 tcgccgtcct cgccgacgag ctcggtctcc tcctcgacct cctcgggctc ctcctcgacc
     4801 gtcgggaaga tgacggtggc gaccaccgtc tcctggaggt cgtccaggaa ctccacgccc
     4861 tcgggggcgg gaacggactc gagcatgagc gtgtcgccca tgtccatggc ggacacgtcc
     4921 accacgatca ggtccgggat gtcggtgggc agcgcctcga tgttgatctc gcgcgtggcc
     4981 aggtccagga cgccgccctc cttgacgccg ggggcgtcct cgacgccctc cagctccagg
     5041 gcgaccgtgg cgtggatctt ctccttgagg ttgacctcga ggaagtcgac gtgcatcacg
     5101 tcgccgcgca cggggtggtc ctggcggtcc ttcaggatgg ccgggaccga cttgtcggcg
     5161 ccgatcttga tgtcgaacag ggccgcgccc tcgttcagca ggcggcgcag ctcgatggcc
     5221 cccaccttca gcgagacggg gtccggatgg ctggcgccgt acaggatgcc gggcacctgg
     5281 ccgctacggc gcaggcgccg tgcttcgcgg gatccgcgct cggagcgctc ttctactgca
     5341 agggagggtc ggtcatcagc catagcgacc ggcgatggta gcgctccggc ttagaatccg
     5401 gcgtatgggc ctgctctcac gctcggtccc gaccacg
//
LOCUS       DASUPW010000261         2235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1998955, whole genome shotgun sequence.
ACCESSION   DASUPW010000261 DASUPW010000000
VERSION     DASUPW010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1998955"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2158)
                     /locus_tag="VFY44_05940"
     CDS             complement(<1..2158)
                     /locus_tag="VFY44_05940"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX5910015.1"
                     /translation="MSGERLELPITGMTCASCANRVEKQLNTLEGVTATVNYATERAT
                     VDFRPDAVDPSDLVGAVQAAGYDAQLPAAEEDPASAGEEAAADPLLRRLVASAALSLP
                     VLAISMVPALQFDNWQWLALNLATPVVLWGAWPFHRAAWTNLRHGQATMDTLVSVGVL
                     AAWLWSLYALFIGQAGMPDMRMAFDLIPERGEGADHIYLETAAVVTTLILAGRWLEAR
                     AKRRAGAALTALLELGAKDVEVLDDDGSARRIPTADLAVGTRFLVRPGQKVATDGVVE
                     QGNSALDKSLLTGEPVPVEVGPGDAVVGATVNAGGRLVVRATRVGSDTTLGQIARLVS
                     DAQAGKAPVQRLADRVSAVFVPVVFVLATATLGFWLGTGESTAFAFTTAVAVLIIACP
                     CALGLATPTALMVGSGRGAQLGLLIKGPEVLESTRRVDTVLLDKTGTVTTGRMALADV
                     ATAAGTARGEALRLVGAVEDASEHPVARAIADAARAEADGALPVAEHFAARSGLGAQG
                     VVDGAMVVVGRPALLAEHGLLVPPALTAARTEAEAAGRTAVLAGWQGSARAVLVVADT
                     VKPSSAEAVARLRELGLRPVLLTGDNRVTAEAVAHQVGIEEVVAEVLPADKAAAVREL
                     QEAGRVVAMVGDGVNDAPALAQADLGLAIGTGTDVAIEASDLTLVSGDLRAAADAIRL
                     SRATLRTIKQNLGWAFGYNVAAIPLAAVGLLNPVLAG"
BASE COUNT          282 a          879 c          814 g          260 t
ORIGIN      
        1 ctccggccag caccgggttc agcaggccga ccgccgccag cgggatggcc gcgacgttgt
       61 agccgaaggc ccagcccagg ttctgcttga tggtccgcag cgtggcgcgg ctcaggcgga
      121 tcgcgtcggc ggcggcgcgc aggtcgcccg agaccagcgt caggtccgat gcctcgatgg
      181 ccacgtcggt cccggtgccg atggccaggc ccagatccgc ctgggccagc gcgggcgcgt
      241 cgttcacgcc gtcgccgacc atggccacca cgcggccggc ctcctgcagc tcgcggaccg
      301 cggccgcctt gtcggccggc agcacctcgg ccacgacctc ctcgatgccc acctgatggg
      361 ccacggcctc ggcggtcacc cggttgtcgc cggtcagcag caccgggcgc aggcccagct
      421 cgcgcagccg cgccacggcc tcggccgagc tgggcttgac ggtgtcggcc accaccagca
      481 ccgcgcgggc gctgccctgc cagccggcca gcacggcggt gcggccggcg gcctcggcct
      541 cggtccgggc cgccgtcagc gccgggggga cgagcaggcc gtgctcggcc agcagcgccg
      601 gccggcccac cacgaccatc gccccgtcca cgaccccctg cgcccccagg ccggagcggg
      661 cggcgaagtg ctcggccacg ggcagggccc catcggcctc ggcccgcgcg gcgtcggcga
      721 tggcgcgcgc gaccggatgc tcggatgcgt cctcgaccgc gcccaccagc cgcagggcct
      781 ccccgcgggc cgtcccggcg gcggtggcca cgtcggccag cgccatccgg ccggtggtga
      841 ccgttccggt cttgtccagc agcacggtgt ccacgcgccg ggtcgactcc agcacctccg
      901 ggcccttgat cagcagcccc agctgcgcgc cgcggccgct gcccaccatc agcgcggtgg
      961 gcgtggccag gcccagcgca cacgggcagg cgatgatcag cacggccacc gccgtggtga
     1021 aggcgaacgc ggtgctctcg ccggtgccca gccagaagcc cagcgtggcg gtcgcgagca
     1081 cgaagacgac ggggacgaac acggccgaga cgcggtccgc cagacgctgg accggcgcct
     1141 tgccggcctg ggcgtcggag accagccggg cgatctggcc cagggtggtg tcagacccca
     1201 cccgggtggc gcggaccacc aaccggccgc cggcgttcac ggtggccccg acgacggcgt
     1261 caccggggcc cacctcgacc gggacgggct cgccggtcag caggctcttg tccagggccg
     1321 agttgccctg ctcgaccacg ccgtcggtgg ccaccttctg gcccggccgg accaggaagc
     1381 gcgtgcccac ggccaggtcc gcggtcggga tccggcgcgc ggatccgtcg tcgtccagca
     1441 cctcgacgtc cttggcgccc agctcgagca gcgcggtcag cgcggctccg gcgcgccgct
     1501 tggcccgcgc ctcgagccag cgcccggcca ggatcagcgt ggtcaccacg gcggccgtct
     1561 ccaggtagat gtggtcggca ccctcgcccc gctcggggat caggtcgaac gccatccgca
     1621 tgtccggcat gcccgcctgg ccgatgaaca gcgcgtacag cgaccacagc caggcggcca
     1681 ggacgccgac gctgaccagc gtgtccatgg tggcctggcc gtggcgcagg ttggtccagg
     1741 cggcgcggtg gaacggccac gcgccccaca gcaccacggg cgtggccagg ttcagcgcca
     1801 gccactgcca gttgtcgaac tgcagcgccg gcaccatcga gatggccagc accggcagcg
     1861 acagcgccgc gctggccacc agccggcgca gcagggggtc ggcagcggcc tcctcgccgg
     1921 ccgacgcggg gtcctcctcg gccgccggca gctgcgcgtc gtagcccgcg gcctgcacgg
     1981 ccccgaccag gtcggacggg tccaccgcgt ccgggcggaa gtccacggtg gcgcgctcgg
     2041 tggcgtagtt caccgtggcg gtcaccccct cgagggtgtt cagctgcttc tcgacgcggt
     2101 tggcgcacga ggcgcaggtc atgccggtga tgggcagctc caggcgctcg ccgctcatcg
     2161 cccgacctcg tgggtgaagg cggccgtgcg cacgcggccg gcgctcttga cctgcacgaa
     2221 cacccggtag cggcc
//
LOCUS       DASUPW010000262         4242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_59757, whole genome shotgun sequence.
ACCESSION   DASUPW010000262 DASUPW010000000
VERSION     DASUPW010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59757"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VFY44_05945"
     CDS             complement(<1..324)
                     /locus_tag="VFY44_05945"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX5910016.1"
                     /translation="MRQPVIIDYVRTPFGRYAGALSPVRPDDLAATVLAEAVRRTGID
                     AAGVDEVLLGDTNQAGEDNRNVARMAALLAGFPVEVPAATVNRLCASGLEAVSQAARA
                     VRSDEG"
     gene            365..667
                     /locus_tag="VFY44_05950"
     CDS             365..667
                     /locus_tag="VFY44_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910017.1"
                     /translation="MDEFSLFVLGAFVVVILIFLAIGKWYPGSGAEQVDWKPTRSPEL
                     EAQLEIDDVEQMIEAQNVRRRASGRPEITEEGVQAQVHEDERWRREQANLDDDGRA"
     gene            677..1162
                     /locus_tag="VFY44_05955"
     CDS             677..1162
                     /locus_tag="VFY44_05955"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX5910018.1"
                     /translation="MARVLIVGCGCRGQELARDLGAAGHAVRGTTRDAAKTADIEAAG
                     AEAVVADPDRLGTLLPLMAGVTVICWLMGTAEGDNVPALHGDRLDSLLGKLVDSGVRG
                     LVYEAAGSVDAGLLDDGAARVQAAGSVNSMPVAVIATAPSAHADWLAAARAAVDQVLA
                     G"
     gene            complement(1159..2334)
                     /locus_tag="VFY44_05960"
     CDS             complement(1159..2334)
                     /locus_tag="VFY44_05960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HEX5910019.1"
                     /translation="MSALPGWAEWQPGSEYTLGVEEEVMLLNPGDWSLAQQIDRVLPT
                     LSEDLAAHVTPETHRSALEMGTGIHPTVASAAEELLHLRRTLEAELEPLGLRCASAGT
                     HPFTVWHETVLSSGDRYDTVYDSMRELARREPTFALHVHVGVPDPEAAIRLFNRLRAH
                     LPLLLALSANSPFWQGRDSGLSSARTSLFQAFPRVGVPRAFADYGDWVDTVGLLVRSD
                     AFPDASYLWWDVRPVPRFGTVEVRVMDAQTTVTETAALVALVQCIARLEATDGFASPL
                     LVQSTELVSENRFIAARDGMAGSMIDPVSESRVPVTTVLEELLEACAPHARELGCEAE
                     LEPLSWMAQADGARRQLNLARRAEKLPGLVGRLADSFTEERPAGPDQESSLAPGPPG"
     gene            complement(2331..3095)
                     /locus_tag="VFY44_05965"
     CDS             complement(2331..3095)
                     /locus_tag="VFY44_05965"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX5910020.1"
                     /translation="MRPLIAVTTSEMRGVGSHAQTPEGEPPSRELALGLSYPDSVAAA
                     GGVPLVVPPMDHAAIEPLLNQVGGVCLTGGPDLDPSSYGAEAHPQLGPIESEVDRFEL
                     ALAHQADLLGLPVLAICRGLQTLNVARGGTLHQHVPDDFGGDHRQTEPGTEVTHEVSI
                     RPDSRLAEMMGGHHAEVNSFHHQAIDVLGRGLRPVAWAPDGVIEGVEDARRGFLVGVQ
                     WHAETLTHRPEHAALFSGLVAAAASGRGRQRVRSVA"
     gene            complement(3092..>4242)
                     /locus_tag="VFY44_05970"
     CDS             complement(3092..>4242)
                     /locus_tag="VFY44_05970"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX5910021.1"
                     /translation="GLVQRARALTAFLADAYGEARIVGAGRIPARVIESAVYHEPLAH
                     GLELRHDGFLAGLDLVRDLDGALAVLEDNTRTPSGLGYAIGARTAVDGELGDLAPAGR
                     LDVQEGYGMVAAALKAAAPDGVDEPVVVLLSDGPGNSAWHEHQQYAARMDVPLVTPDD
                     LAVRAGRLHARIGDERSRPVDVVYRRTDEDRLSDEQGRPTWLAELLLPPAREGTLTVV
                     NPLGSGLADDKLVHAYVEDMVRFYLDEEPLLPSVTTYDLGDPDGLTDAMERIGELVVK
                     PRDGYGGIGVVLCRECSAGELAEVESRVRADPGSFVAQELVSISTHPTVIDGRLAPRH
                     VDLRPFVVGGGHASTTVPGGLSRVAFGAGEMVVNSSRNGGGKDTWVMA"
BASE COUNT          558 a         1549 c         1595 g          540 t
ORIGIN      
        1 gccctcgtcc gagcgcacgg cccgggcggc ctggctcacg gcctccagcc cggaggcgca
       61 cagccggttg accgtggccg ccggcacctc caccgggaag ccggccagca gggccgccat
      121 gcgggccacg ttgcggttgt cctcgccggc ctggttggtg tcgcccagca ggacctcgtc
      181 gacgccggct gcgtcgatgc cggtgcggcg gacggcctcg gccagcacgg tggccgccag
      241 gtcgtcgggg cggacggggg acagggcgcc cgcgtagcgg ccgaagggcg tccggacgta
      301 gtcgatgatc accggttggc gcactcgccc gagcctatcg gcggttcggg gctaggctcc
      361 gtccatggac gagttcagct tgttcgtgct gggggcgttc gtcgtcgtca tcctgatctt
      421 cctggccatc gggaagtggt atcccggctc gggggcggag caggtggact ggaagcccac
      481 gcgctcgccg gagctcgagg cgcagctgga gatcgacgac gtggagcaga tgatcgaggc
      541 gcagaacgtg cgccgccggg ccagcggccg gccggagatc acggaggagg gggtacaggc
      601 gcaggtgcac gaggacgagc gctggcgccg cgagcaggcc aacctggacg acgacggccg
      661 cgcgtaggct gcagccatgg cccgcgtgct catcgtcggt tgcggctgcc gcgggcagga
      721 gctggcgcgc gacctgggcg cggccggtca cgcggtccgc gggaccacgc gtgacgcggc
      781 gaagacggcg gacatcgagg ccgcgggggc cgaggccgtg gtggccgacc ccgaccggct
      841 gggcacgctg ctgccgttga tggccggcgt cacggtgatc tgctggctga tgggcaccgc
      901 cgagggcgac aacgtgccgg ccctgcacgg cgaccggctg gacagcctgc tgggcaagct
      961 cgtggacagc ggggtgcgcg gcctggtcta cgaggcggcc gggagcgtgg acgcgggcct
     1021 cctggacgac ggcgccgccc gcgtccaggc cgccgggagc gtgaacagca tgccggtcgc
     1081 ggtcatcgcc acggcgccgt cggcgcacgc cgactggctg gccgcggccc gggcggcggt
     1141 ggaccaggtc ctggccggct agcccggcgg cccgggcgcg aggctcgatt cctggtccgg
     1201 ccccgcgggc cgctcctcgg tgaaggagtc ggccagccgg cccaccagcc ccggcagctt
     1261 ctccgcccgg cgggccaggt tcagctggcg ccgggcgccg tcggcctggg ccatccacga
     1321 cagcggctcc agctcggcct cgcagcccag ctcacgggcg tgcggggcac acgcctccag
     1381 gagctcctcc agcaccgtgg tgaccggcac ccgcgactcg gacaccgggt cgatcatcga
     1441 ccctgccatc ccgtcgcgcg cggcgatgaa gcggttctcg gagaccagct cggtgctctg
     1501 caccagcagt ggcgaggcga acccgtcggt ggcctccagc cgcgcgatgc actggacgag
     1561 cgccaccagc gcggccgtct cggtcacggt cgtctgggcg tccatgacgc gcacctccac
     1621 ggtcccgaag cggggcacgg gacgcacgtc ccaccacagg tagctggcgt ccgggaaggc
     1681 atcgctgcgc acgagcagcc cgacggtgtc cacccagtcg ccgtagtccg cgaaggcccg
     1741 cgggaccccg acccggggga acgcctggaa gagcgacgtc cgcgccgagc tcaggccgct
     1801 gtcacgcccc tgccagaacg gggagttggc ggacagggcc agcagcagcg gcaggtgggc
     1861 ccgcagccgg ttgaacaggc ggatggcggc ctcgggatcg gggaccccga cgtgcacgtg
     1921 cagcgcgaag gtcggctcgc gccgcgccag ctcgcgcatg gagtcgtaga cggtgtcgta
     1981 gcggtccccg ctggacagga cggtctcgtg ccacacggtg aacgggtggg tccccgcgct
     2041 ggcgcaccgg agccccagcg gctccagctc ggcctcgagc gtgcggcgca gatgcagcag
     2101 ctcctcggcg gcggaggcca cggtggggtg gatcccggtg cccatctcca gcgcggagcg
     2161 atgggtctcc ggcgtcacgt gcgccgccag gtcctccgac agggtgggca gcacgcgatc
     2221 gatctgctgc gccagcgacc agtcgccggg gttcagcagc atgacctcct cctcgacccc
     2281 gagcgtgtac tcgcttccgg gctgccactc ggcccagccc gggagcgcgc tcatgcgacg
     2341 ctgcgcaccc gctggcggcc tcgtccggac gccgcggccg ccaccaggcc ggagaacagc
     2401 gccgcgtgct cgggccgatg ggtcagcgtc tcggcgtgcc actggacgcc gacgaggaac
     2461 ccgcggcggg cgtcctcgac gccctcgatc acgccgtcgg gcgcccaggc cacgggccgc
     2521 agaccgcgcc ccaggacatc gatggcctgg tggtggaagg agttgacctc ggcgtggtgg
     2581 ccgcccatca tctccgccag ccggctgtcg ggccggatcg acacctcgtg cgtgacctcg
     2641 gtgccgggct cggtctggcg gtggtctccg ccgaagtcgt cgggcacgtg ctggtgcagc
     2701 gtccctccgc gtgcgacgtt cagggtctgg agcccgcgac agatggccag cacgggcagg
     2761 cccagcaggt cggcctggtg ggccagggcc agctcgaagc ggtcgacctc ggactcgatc
     2821 gggcccagct ggggatgggc ctcggccccg tacgaggagg ggtccaggtc gggccccccg
     2881 gtcaggcaga cgccgcccac ctggttcaag agcggctcga tggccgcgtg gtccatgggc
     2941 ggtaccacca gcggcacgcc gccggcggcc gccacggagt ccgggtagct caggcccagg
     3001 gccagctcgc gcgagggcgg ctcgccctcg ggtgtctggg cgtggctgcc cacaccgcgc
     3061 atctccgagg tcgtgaccgc tatcaggggc ctcacgccat cacccatgtg tccttgccac
     3121 ctccgttacg ggagctgttc accaccatct cgccggcacc gaaggcgacc ctgctcaggc
     3181 cgcccggcac cgtggtcgac gcgtggcctc caccgaccac gaacggtcgc aggtccacat
     3241 gacgtggcgc cagccgcccg tcgatcacgg tcggatgcgt ggagatcgag accagctcct
     3301 gggccacgaa cgagccggga tccgcgcgca cccgggactc gacctcggcc agctcgccgg
     3361 ccgagcattc gcggcacagc accacgccga tcccgccgta gccgtcgcgg ggcttgacca
     3421 ccagctcgcc gatccgctcc atggcgtcgg tgagcccgtc ggggtcgccc aggtcgtagg
     3481 tcgtcaccga gggcagcagc ggctcctcgt ccaggtagaa gcggaccatg tcctccacgt
     3541 aggcgtgcac cagcttgtcg tcggccaggc cggagcccag cgggttgacc acggtcaggg
     3601 tgccctcacg ggccggcggg agcaggagct cggccagcca cgtgggccgg ccctgctcgt
     3661 cgctcagccg gtcctcgtcg gtgcggcggt agaccacgtc cacagggcgc gagcgctcgt
     3721 cgccgatgcg ggcgtgcagg cgcccggctc gcaccgcgag gtcgtcgggg gtgaccagcg
     3781 gcacgtccat gcgggccgcg tactgctggt gctcgtgcca ggcgctgttg cccggtccgt
     3841 ccgagagcag caccacgacg ggctcgtcca cgccatcggg cgcggcggcc ttcagcgcgg
     3901 ccgcgaccat cccgtacccc tcctgcacgt ccaggcgccc ggcgggggcc aggtcgccca
     3961 gctcgccgtc gaccgccgtc cgggcgccga tcgcgtagcc cagtccggac ggggttcgcg
     4021 tgttgtcctc caggaccgcg agggcgccgt ccaggtcgcg caccaggtcc aggccggcca
     4081 ggaacccgtc gtggcgcagc tccaggccgt gtgccagcgg ctcgtggtag acggcggact
     4141 cgatcacccg ggccgggatg cggcccgcgc ccacgatccg cgcctcgccg taggcatcgg
     4201 ccaggaacgc ggtcagggct cgggcccgct ggaccaggcc gg
//
LOCUS       DASUPW010000263         3259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_892125, whole genome shotgun sequence.
ACCESSION   DASUPW010000263 DASUPW010000000
VERSION     DASUPW010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892125"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="VFY44_05975"
     CDS             complement(<1..1208)
                     /locus_tag="VFY44_05975"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5910022.1"
                     /translation="MRRLTAILVLACALAPAGCDGGITGDRGDATQGGSVLVGLALAP
                     DSLDPALASSPEALQALWLAYTPPMTYRRAEGAQGTQLMPGLAQSKPEPLDGSTAFRF
                     TLRRDLTYSDGRSVLAADVPRAIRRSRALNPAARRALAGIEDIEVDERERTVRIQLRT
                     PDPEFPDLLTTLWTAPVPPGTPTRDLSRTPVAGVGPYRLEAPTRGRSYALTRRRDFRL
                     PGIPAGNADSVAGTVVASPSRRTNQTLGGLLDVTQGEPPVARLPQIRSEYKSRYREFA
                     TLTSLYVAFDLDRPPFTDQDVRRAVGFALDLRALGQIQDGFLSPGCNAIPPQVPGHVE
                     IDPCPFGTRDGDPDLVRAEQLVRGSRERRAPVIVDGGATPRAAALARYGVQTLEKIGL
                     RARLARTARDR"
     gene            complement(1205..1960)
                     /locus_tag="VFY44_05980"
     CDS             complement(1205..1960)
                     /locus_tag="VFY44_05980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HEX5910023.1"
                     /translation="MSAPDTARARAREIDRELLRRLHEDGDESARERLVQRHLPLVRS
                     LARRYSGRGESLEDIEQVGAIGLIKAIDRFELGREVSLATYATPTVVGEIKRHFRDKG
                     WAIRVPRALQELNARMSATIDRLTSRLGRSPSVAEIAAELKTTPEEVLEALEVGTAYT
                     TLSLSTGPGTEEESDPMESLGTEDEEFSRSEDRASLAPALALLPPREQEILRMRFEEG
                     LPQTQIAERVGLSQMHVSRLIRKALGAMRDELQ"
     gene            complement(1957..2337)
                     /locus_tag="VFY44_05985"
     CDS             complement(1957..2337)
                     /locus_tag="VFY44_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910024.1"
                     /translation="MSDRIVLEAPLDGDFGSVVRLIVGGIAAGADLGFEEMDDLQLAI
                     ERLYAESGGQERLTIDFELSPGRVRVRLGPLRERGLADALQREHEHGALSLAGVLDTV
                     VDSYGVEEAADGRLFIRLVKLVAP"
     gene            complement(2330..>3259)
                     /gene="mnmA"
                     /locus_tag="VFY44_05990"
     CDS             complement(2330..>3259)
                     /gene="mnmA"
                     /locus_tag="VFY44_05990"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF001138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX5910025.1"
                     /translation="TDGEKSCCSPQAVTGARALAHRMGIPHFTLDLRERFRADVVDDF
                     LAGYAGGSTPNPCVRCNGLVRFDAMLDLARDLGAPRLVTGHYARIAEDGQGPLVRAGA
                     DPAKDQSYVLARLTGDELERLRFPLGGLEKPHVRELAGAAGLPVARKPESQDLCFLAG
                     TNGPDFMSRHGAPRRRAGAIVDRAGTVLGEHDGHQRFTVGQRRGIGVAGPEPLYVLAK
                     DAHRNRVVVGTRAELGVRSVAVDPLVLHRPAQRATSVRLRYHAPTVPCVVHDGRSIAL
                     GREVVRPAPGQTACLMDGDLVVGWGTMRAQADV"
BASE COUNT          402 a         1220 c         1231 g          406 t
ORIGIN      
        1 cggtcgcggg cggtgcgcgc caggcgcgcg cgcagcccga tcttctccag ggtctgcacg
       61 ccgtagcggg ccagcgcggc cgcgcgcggc gtcgcgcccc cgtccacgat gaccggcgcg
      121 cgccgctcgc gcgacccgcg caccagctgc tcggcacgca ccaggtcggg gtcgccgtcg
      181 cgggtgccga aggggcaggg gtcgatctcc acgtggccgg gcacctgggg cgggatggcg
      241 ttgcagccgg gtgacaggaa gccgtcctgg atctggccca gggcgcgaag gtccagcgcg
      301 aaccccaccg cccggcgcac gtcctggtcg gtgaacggcg gccggtccag gtcgaaggcc
      361 acgtacaggg aggtcagcgt ggcgaactcg cggtagcgcg acttgtactc ggacctgatc
      421 tgaggcaggc gcgcgacggg cggctccccc tgggtcacgt ccaggagccc gccgagggtc
      481 tggttggtgc ggcggctcgg gctggccacc acggtgccgg ccaccgagtc cgcgttcccg
      541 gccgggatgc ccggcagccg gaagtcgcgg cgccgggtca gcgcgtagga gcgaccgcgc
      601 gtcggggcct ccagccggta ggggcccaca cccgcgaccg gcgtgcgcga caggtcgcgg
      661 gtcggtgtgc ccggcggcac cggcgccgtc cacaacgtgg tcagcaggtc ggggaactcg
      721 gggtcgggtg tcctcagctg gatgcgcacc gtgcgctcgc gctcgtcgac ctcgatgtcc
      781 tcgatgcccg ccagcgcgcg gcgcgccgcc gggttcagcg cccgcgaccg ccggatggcg
      841 cgcgggacgt cggcggccag gacggagcgg ccgtcggagt aggtcaggtc cctgcgcagg
      901 gtgaagcgga acgcggtgga gccgtcgagc ggctccggct tggactgggc caggcccggc
      961 atcagctggg tgccctgcgc gccctcggcg cggcggtagg tcatcggcgg cgtgtaggcc
     1021 agccacaggg cctgcagcgc ctcgggcgag gaggccagcg ccgggtccag cgagtccggc
     1081 gccagggcca ggccgaccag cacgctgccg ccctgggtgg cgtcgccgcg gtcaccggtg
     1141 atgccgccgt cgcagcccgc cggcgcaagc gcgcaggcca ggaccaggat ggctgtgagg
     1201 cgtctcactg gagctcgtcg cgcatggcgc ccagcgcctt gcggatcagc cgcgacacgt
     1261 gcatctggga cagcccgacg cgctcggcga tctgggtctg gggcaggccc tcctcgaagc
     1321 gcatgcgcag gatctcctgc tcccgcgggg gcagcagcgc gagggcgggc gccagcgagg
     1381 cgcggtcctc gctgcgcgag aactcctcgt cctcggtgcc caggctctcc atcgggtccg
     1441 actcctcctc ggtgccgggc ccggtggaca gcgaaagcgt ggtgtaggcc gtgcccacct
     1501 ccagcgcctc gagcacctcc tcgggcgtgg tcttcagctc cgcggcgatc tcggccacgc
     1561 tgggcgagcg gcccaggcgc gaggtcagcc ggtcgatcgt cgccgacatg cgggcgttca
     1621 gctcctgcag cgcgcgcggc acgcggatgg cccagccctt gtcacggaag tggcgcttga
     1681 tctcacccac caccgtcggc gtcgcgtacg tggccagcga cacctcgcgg cccagctcga
     1741 agcggtcgat cgccttgatc agcccgatcg cgcccacctg ctcaatgtcc tccagcgact
     1801 ccccgcggcc ggagtagcgc cgggccagcg agcgcaccag cggcaggtgc cgctgcacca
     1861 ggcgctcgcg ggcggactcg tcgccgtcct cgtgcaggcg ccgcagcagc tcacggtcga
     1921 tctcgcgcgc ccgcgcccgg gccgtgtccg gagcgctcat ggggcgacca gcttcaccag
     1981 ccggatgaac agcctgccgt cggcagcctc ctccacgccg taggagtcca cgacggtgtc
     2041 cagcaccccg gccaggctca gggcgccgtg ctcgtgctcg cgctggaggg catcggccag
     2101 cccgcgctcg cgcagcggtc ccaggcgcac gcgcacgcgc ccgggcgaga gctcgaagtc
     2161 gatggtcagc cgctcctggc ccccggactc ggcgtacagg cgctcgatgg ccagctggag
     2221 gtcgtccatc tcctcgaagc ccaggtcggc gccggcggcg atgccgccca cgatcaggcg
     2281 gacgacggac ccgaagtcgc cgtccagcgg cgcctccagg acgatccggt cagacatcgg
     2341 cttgcgctcg catggtgccc caccctacga ccaggtcccc gtccatcagg cacgcggtct
     2401 gccccggggc gggccgcacc acctcgcgcc cgagcgcgat cgaccgcccg tcgtggacca
     2461 cgcagggcac ggtgggcgcg tggtagcgca ggcgcacgct cgtggcccgc tgcgccggcc
     2521 ggtgcaggac cagcgggtcc acggccaccg acctcacccc cagctccgcg cgcgtgccca
     2581 ccacgacccg gttgcggtgc gcgtccttgg ccaggacgta cagcggctcg gggccggcga
     2641 caccgatccc acggcgctgg ccgaccgtga agcgctggtg gccgtcgtgc tcgcccagca
     2701 cggtgcccgc ccggtcgacg atcgcaccgg cgcggcggcg gggagcgccg tggcggctca
     2761 tgaagtcggg cccgttcgtg ccggccagga agcacaggtc ctggctctcg ggcttgcgcg
     2821 ccacgggcag gcccgcggcc cccgccagct cgcgcacgtg tggcttctcg aggcctccga
     2881 gggggaagcg caggcgctcg agctcgtcgc cggtcaggcg ggccagcacg tagctctggt
     2941 ccttggccgg gtcggcgccg gcgcggacca gcggcccctg cccgtcctcg gcgatgcggg
     3001 cgtagtgccc ggtcaccagc cgcggggcgc ccaggtcgcg ggccaggtcg agcatggcgt
     3061 cgaagcgcac cagcccgttg cagcgcacgc acgggttggg cgtgctgccg cccgcgtagc
     3121 cggccaggaa gtcgtcgacc acgtcggccc ggaagcgctc gcgcaggtcc agcgtgaagt
     3181 gcgggatgcc catgcggtgc gccagggcgc gggcgccggt cacggcctgg ggcgagcagc
     3241 agctcttctc gccgtcggt
//
LOCUS       DASUPW010000264         5407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_753841, whole genome shotgun sequence.
ACCESSION   DASUPW010000264 DASUPW010000000
VERSION     DASUPW010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5407
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753841"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VFY44_05995"
     CDS             complement(<1..584)
                     /locus_tag="VFY44_05995"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX5910026.1"
                     /translation="MRAVACTRGELAVGDRPDPVPGRGQLLLDVTRCGICGSDLHARA
                     HADQVAGLMSELEYEDFMRSDQEVVFGHEFCGEVVAQGPACRGTAPEGTPVVAVPLLR
                     GADGVHPTGLSAKAPGAYAERLVVEESLAFPVPNGLAPDVAVLTEPMAVGWHAVRRSE
                     IGKGDVAVVIGCGPIGLAVICLLKARGIEPVVAS"
     gene            682..1845
                     /locus_tag="VFY44_06000"
     CDS             682..1845
                     /locus_tag="VFY44_06000"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HEX5910027.1"
                     /translation="MNRLLAVTGFLAVLLVFATTGPATAAPSAGAAAAARPDLGLTKK
                     SVVRLKSGRVSGAFVVRNSGRAGARRFRAALSVKVAKKYRVIKRYKLRALKRKRSRRV
                     KVSVKLPAGLPAGNLPLRLCVDTTGRLKERSEKNNCRRVGTAKVKGGSTPVPPAGPVD
                     SRPRNPVPFTKDTVFKLDNYWITVPQAYDASHNTPITLFVWSHGCGGMSSGDIATVSP
                     GGTAQTWIAVAADGREGLCWDVNADPALIMNVVATVKTHFNINPRRVVLGGYSSGGDL
                     SYRTAFTNARTFAGVLAENTAPFRDTGLSQQAAISGAAWKFNVVHLAHTEDDTYPIDT
                     VRNEVNALKGAGFPATLIERPGTHFDNDTPNSGTDHDLKTLLLPRLNDGWLSP"
     gene            complement(1842..3011)
                     /locus_tag="VFY44_06005"
     CDS             complement(1842..3011)
                     /locus_tag="VFY44_06005"
                     /inference="COORDINATES: protein motif:HMM:NF015541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HEX5910028.1"
                     /translation="MLRLPLLALSLALVAAAPASAATTKPLKAPSGLAFYKPPAKLPG
                     KTHGDVIWSRPATGKSRLSGAGTRLVLYRSVGARGKPVAVSGLVSVPRGKAPKGGFPV
                     ISWAHGTTGIADKTAPTRLAGASEYIRTSLLEAWIKKGYAVVRTDYEGLGTPGVHPYL
                     IGRSEGRSVLDIVRAARELDDDVSGRLAISGHSQGGHAALFAAALAPRWTPELTLRGT
                     APFAPASQLDEQVPLVRALKAPGPLSVLVGLIGAGIDAGEPQVRLRTLLSPRALAVLP
                     QADQRDMGELARPDSFGGIAPADLFRPDADPSALVRALAKNDPSDLKIRTPVRVEQGT
                     ADNTVIPVFTDKMVATLRKAGTPLIYVKTKGQSHTGIVRATAPDVRRWIDSKLKR"
     gene            3098..3301
                     /locus_tag="VFY44_06010"
     CDS             3098..3301
                     /locus_tag="VFY44_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910029.1"
                     /translation="MKVVLKIVAVLLIAIAAFLVYAVIAAATSEEGARVGVCIGYAVA
                     AVVLGFASVKLWNFRKTSTAPAA"
     gene            3423..4082
                     /locus_tag="VFY44_06015"
     CDS             3423..4082
                     /locus_tag="VFY44_06015"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX5910030.1"
                     /translation="MALTIGDTAPDFEAETTEGRINFHDWIGDGWAVLFSHPRNFTPV
                     CTTELGYMASIKPDFDSRNTKIIGLSVDPIENHEKWAKDIAETQGTAPNYPIIADSDF
                     AVSKAYGMLPPDVEGDPTERTPAQNATLRNVFVIGPDKKVKLVLIYPMTTGRNFDEVL
                     RVIDSLQLTSNEAVATPAQWQQGDDVIIAGSVSDDQAKEKYPDGWDAPRPWLRIVPQP
                     K"
     gene            4263..>5407
                     /locus_tag="VFY44_06020"
     CDS             4263..>5407
                     /locus_tag="VFY44_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX5910031.1"
                     /translation="MPQPRPLVIPRWIVGAMMAVVVVLGCASAWQLAESQSDSRESLR
                     NRFADRAPVATAVVDGLFKSIFTGQAQDLDPKVFGTPTVAPAALDAMARRSKNVYVAV
                     IDARGRAVGASARAPRRRPGLRAYEREALRMGYGLSDVQPGRVPTILNVVRFSTPSGP
                     RLLLSASPEKAYAEFLQTTLKPLVRSDDDAAFLLDGNGAAIAAVPRARGKADMRPVAG
                     GRRSGEFSTPEGERFFASAPVDGSSWRTVLTVPESALYAPAGGLGRWLPWGTLALLVT
                     ALLGMAWLVRRLSETGSRLSRSNTDLESANANLARSNADLEQFAYVASHDLSSPLRSV
                     AGFSQMLAKRYRGRLDSDADTWIGFVEQGVSRMQRVIDDLLLYSRVDR"
BASE COUNT          781 a         2010 c         1884 g          732 t
ORIGIN      
        1 tcgctggcca ccacgggctc gatcccgcgc gccttgagca ggcagatcac cgcgagcccg
       61 atcgggccgc agccgatgac cacggccacg tcgcccttgc cgatctcgct ccggcgcacg
      121 gcgtgccagc ccaccgccat cggctcggtg aggacggcga cgtcgggcgc gaggccgttg
      181 ggcacgggga aggccagcga ctcctcgacc accagccgct cggcgtacgc gcccggcgcc
      241 ttggcggaga gcccggtggg gtggaccccg tcggcgccgc gcagcagcgg caccgccacg
      301 accggggtgc cctccggcgc ggtcccccgg cacgcgggcc cctgcgcgac gacctcccca
      361 cagaactcgt ggccgaacac gacctcctgg tccgagcgca tgaagtcctc gtactcgagc
      421 tcggacatca gcccggcgac ctggtccgcg tgcgcgcgcg cgtggaggtc ggacccgcag
      481 atgccgcacc gggtgacgtc caggagcagc tgcccgcggc ccggcacggg gtcgggccgg
      541 tctccgaccg ccagctcgcc ccgggtgcag gccaccgccc tcatgggcgg agtatccctc
      601 aggcgcgggg ccgcggggaa cgcctcaggg ggtccccccg aacttttcgc ccgttcgccc
      661 ggttgccctt accgtggtcg cgtgaaccgc cttctcgccg tcaccggatt cctggctgtg
      721 ctcctcgtct tcgcgacgac cgggcccgcc accgccgccc cctcggccgg ggccgccgcc
      781 gcggcgcgcc cggacctcgg gctgaccaag aagtccgtgg tccgcctcaa gagcgggcgc
      841 gtgtccggcg ccttcgtggt ccgcaacagc ggccgcgccg gcgcccgcag gttccgggcc
      901 gcgctgagcg tgaaggtggc caagaagtac cgcgtgatca agcgctacaa gctccgcgcg
      961 ctcaagcgca agcgctcgcg cagggtgaag gtgtcggtca agctgccggc cggcctcccg
     1021 gcgggcaacc tgccgctgcg cctgtgcgtg gacaccaccg ggcggctcaa ggagcgctcg
     1081 gagaagaaca actgccgccg ggtcggcacg gccaaggtca agggcggatc gaccccggtt
     1141 ccgccggcgg gccccgtcga ttcacggccc aggaaccccg tcccgttcac caaggacacg
     1201 gtgttcaagc tcgacaacta ctggatcacc gtgccgcagg cctacgacgc ctcgcacaac
     1261 acgccgatca cgctgttcgt gtggtcgcac ggctgcggcg gcatgagctc gggcgacatc
     1321 gccacggtct cgccgggcgg gaccgcccag acctggatcg ccgtggctgc agacgggcgc
     1381 gagggtctgt gctgggacgt caacgccgac ccggcgctga tcatgaacgt ggtcgccacc
     1441 gtcaagaccc acttcaacat caatccccgc cgcgtggtcc tgggcgggta ctcctcgggc
     1501 ggcgatctgt cctaccggac ggcgttcacc aacgccagga cgttcgccgg cgtgctggcc
     1561 gagaacaccg ccccgttccg cgacaccggc ctgagccagc aggccgcgat ctcgggcgcc
     1621 gcgtggaagt tcaacgtcgt gcacctggcg cataccgagg acgacacgta tcccatcgac
     1681 acggtccgca atgaggtgaa cgcgctcaag ggcgccggct tcccggccac gctgatcgag
     1741 cgaccgggca cccacttcga caacgacacg cccaacagcg gcaccgacca cgatctcaag
     1801 accctgctgc tgccgcgcct gaacgacggc tggctctcgc cctagcgctt gagcttggag
     1861 tcgatccagc gccggacgtc gggcgccgtg gcgcgcacga tcccggtgtg ggactggccc
     1921 ttcgtcttga cgtagatcag cggggtgccc gccttgcgca gcgtggcgac catcttgtcg
     1981 gtgaacaccg ggatgaccgt gttgtcggcc gtgccctgct ccacgcgcac gggggtgcgg
     2041 atcttcaggt ccgacgggtc gttcttcgcc agtgcccgca ccagcgccga gggatcggcg
     2101 tcgggccgga acaggtccgc cggcgcgatg ccgccgaacg agtcgggccg tgccagctcg
     2161 cccatgtcgc gctggtcggc ctggggcagg acggccaggg cgcgcgggct gagcagcgtg
     2221 cgcagcctga cctgcggctc accggcgtcg atgccggcgc cgatcaggcc caccagcacc
     2281 gacagcgggc ccggcgcctt gagcgcgcgc accagcggca cctgctcgtc cagctgggac
     2341 gcgggcgcga acggggcggt gccgcggagc gtcagctcgg gcgtccaccg gggggccagg
     2401 gccgccgcga acagcgcggc gtggccaccc tgcgagtgcc ccgagatggc cagccggccc
     2461 gagacgtcgt cgtccagctc gcgcgcggcg cgcacgatgt ccagcaccga gcgtccctcc
     2521 gagcggccga tcaggtacgg gtgcacgccc ggggtgccca gcccctcgta gtccgtgcgc
     2581 acgaccgcat agcccttctt gatccaggcc tccagcagcg acgtgcggat gtactcgctg
     2641 gctccggcca gccgcgtcgg agcggtcttg tcggcgatgc ccgtggtgcc gtgcgcccac
     2701 gagatcacgg ggaacccgcc cttgggcgcc ttgcccctgg ggaccgagac cagcccggag
     2761 accgcgaccg gcttgcccct ggcgcccacc gaccggtaca gcaccagccg cgtgccggcg
     2821 ccgctgagcc tgctcttgcc ggtggccggc cgggaccaga tcacgtcgcc gtgggtcttg
     2881 cccggcagct tggcgggtgg cttgtagaag gccaggcccg agggcgcctt gagcggcttg
     2941 gtcgtggcgg ccgatgcggg cgcggcggcc acgagggcca gggaaagggc gagcaggggg
     3001 agtcggagca tcagccgagt attgctggcg gtggatgctc acgtcggcga tggcatctgc
     3061 cgatgggggc agacctcaac cgaaagcggg aagtcagatg aaggtcgttc tcaagatcgt
     3121 cgccgtgctc ctgatcgcca tcgcggcgtt cctggtctac gcggtgatcg ccgccgcaac
     3181 gtccgaggag ggtgcccgcg tgggcgtctg catcgggtac gccgtcgccg ccgtcgtgct
     3241 gggcttcgcc tcggtgaagc tgtggaactt ccgcaagact tcgacggccc cggcggcctg
     3301 aggccggccc gcagggcccg ccatcgctgc gggccctgcc gggcgccggc ccaggaaaac
     3361 ggtcaatacc cgtcgccttt gagtagatta ggagtcgccc acgaaacgac agggagacct
     3421 ccatggcgct gaccatcggc gacaccgcac cggacttcga ggctgagacc accgaaggcc
     3481 gcatcaactt ccacgactgg atcggcgacg gctgggccgt gctgttcagc cacccgcgca
     3541 acttcacgcc ggtctgcacc accgagctgg gttacatggc cagcatcaag cccgacttcg
     3601 acagccgcaa cacgaagatc atcgggctct cggtcgaccc gatcgagaac cacgagaagt
     3661 gggccaagga catcgccgag acccagggca ccgcgcccaa ctacccgatc atcgccgaca
     3721 gcgatttcgc ggtctcgaag gcctacggca tgctgccgcc cgacgtcgag ggcgatccca
     3781 cggagcgcac cccggcccag aacgccacgc tgcgcaacgt gttcgtgatc ggtccggaca
     3841 agaaggtcaa gctggtcctg atctacccca tgaccaccgg gcgcaacttc gacgaggtgc
     3901 tccgggtcat cgactcgctg cagctgacct cgaacgaggc cgtggccacc ccggcgcagt
     3961 ggcagcaggg cgacgacgtg atcatcgccg gctcggtctc cgacgaccag gccaaggaga
     4021 agtaccccga cggctgggac gccccccggc cgtggcttcg catcgtgccg cagcccaagt
     4081 agccccacct gggccgagcc gccgggaccc gctttgagcg ggttccggcc ctcgagccca
     4141 caccaaaccg cccggcgtta aagcccgcgg cgctcgcgac cgatccccgt gccgggggaa
     4201 gcttcccgcc cccacccgcc cccgtctgct ccgcaggcga ccacacggaa ggaagccggc
     4261 gcatgccaca gccccgcccc ctcgtgatcc cccggtggat cgtcggcgcg atgatggccg
     4321 tggtggtggt gctggggtgc gccagcgcgt ggcagctggc cgagtcccag tccgacagcc
     4381 gcgagagcct gcgcaaccga ttcgccgacc gggccccggt ggccaccgcg gtggtggacg
     4441 gcctgttcaa gtcgatcttc accggccagg cccaggacct ggacccgaag gtgttcggca
     4501 ccccgaccgt cgcccccgcg gccctggacg ccatggcccg ccgcagcaag aacgtctacg
     4561 tggccgtgat cgacgcccgc ggccgggcgg tgggggcctc ggcccgcgcg ccccgccgcc
     4621 gtcccggcct gcgggcctac gagcgggagg cgctgcgcat ggggtacggc ctgtccgacg
     4681 tgcagccggg gcgggtcccc acgatcctga acgtggtccg cttctccacg ccgtcgggcc
     4741 cgcggctgct gctcagcgcg tcgcccgaga aggcctacgc cgagttcctc cagaccacgc
     4801 tgaagccgct ggtgcgctcc gacgacgacg cggcgttcct gctggacggc aacggcgcgg
     4861 ccatcgccgc cgtccccagg gcgcgcggga aggccgacat gcgcccggtg gccgggggcc
     4921 ggcgctcggg cgagttctcg acgcccgagg gcgagcgctt cttcgcctcc gctccggtgg
     4981 acggctcgag ctggcgcacg gtcctgaccg tgcccgagtc cgcgctctac gcgcccgcgg
     5041 gcgggctggg caggtggttg ccgtggggca ccctggcgct gctggtgacc gcgctgctgg
     5101 gcatggcctg gctggttcgc cggctctcgg agaccggcag ccggctcagc cgttccaaca
     5161 ccgacctgga gtcggccaac gccaacctgg cccggtcgaa cgccgacctg gagcagttcg
     5221 cctacgtcgc gtcgcacgac ctgtcctcgc cgctgcggtc ggtggccggg ttcagccaga
     5281 tgctggccaa gcgctaccgc ggccggctgg actcggacgc cgacacgtgg atcgggttcg
     5341 tcgagcaggg cgtcagccgc atgcagcggg tgatcgacga cctgctcctg tactcccgcg
     5401 tggaccg
//
LOCUS       DASUPW010000265         4644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_615419, whole genome shotgun sequence.
ACCESSION   DASUPW010000265 DASUPW010000000
VERSION     DASUPW010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615419"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VFY44_06025"
     CDS             <1..256
                     /locus_tag="VFY44_06025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5910032.1"
                     /translation="TPQKPPLSLFFRLWFDRVVPLLGRLAGDSEAYTYLPSSVRRFPG
                     PEPLAGQLAAAGLVDVGWVLTAGGIIALHHGTVPEAGPPA"
     gene            253..1230
                     /locus_tag="VFY44_06030"
     CDS             253..1230
                     /locus_tag="VFY44_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX5910033.1"
                     /translation="MSTTPATLASVLDAGGPALARALERIETRLGEVTQGHGAELAGH
                     ASGTLSAGGKRLRPVVVVLSGGEGDAVTSAAAAVELLHMATLVHDDVLDAARLRRGVP
                     TVFAVGGRGAATATGDLLFSRAFAELASTRSEAAVQALSTASSALARGELMQRADAWS
                     ATVSAERYLERCELKTASLFAAAARLGATLGDSPAAADGLAGFGRRIGLAFQILDDVL
                     DVSGPAERTGKHRGTDLLDGTVTLPLIIARERDPELAAMDLAAVVTEPAQADAVCDRI
                     ARTGALGTAREQALGYVAEAKAALGDIDMPADRLNALGLVADGVVARYA"
     gene            complement(1261..1692)
                     /locus_tag="VFY44_06035"
     CDS             complement(1261..1692)
                     /locus_tag="VFY44_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910034.1"
                     /translation="MEHHQSDREHVVKRVVLPSGKTIEVVYFGDGDGGEAAAHDRPVL
                     DPADDLHVCIECASRLAYPTAWEEAGSDHWSVTIRCPECETFREGVFSQSAVEAFDEE
                     LDAGTDALMADYRHLMRANMAEEVERFASALDVGAVLPEDF"
     gene            1768..2340
                     /gene="dcd"
                     /locus_tag="VFY44_06040"
     CDS             1768..2340
                     /gene="dcd"
                     /locus_tag="VFY44_06040"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEX5910035.1"
                     /translation="MVLSDRTIRQEIESGRIVVDPFDDSLIQPSSIDVRVDSRYRVFH
                     NQRHPYIDVRKPMDDLTELVEAGGEEPFILHPGEFVLGQTLEKVTLPNDLVARLEGKS
                     SLGRLGLLIHSTAGFVDSGFSGNLTLELSNVANLPITIYEGMPIGQISFMRMDGPVDR
                     PYGSESNTSKYQGQTEPTASRFFENFKDRD"
     gene            2445..2660
                     /locus_tag="VFY44_06045"
     CDS             2445..2660
                     /locus_tag="VFY44_06045"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX5910036.1"
                     /translation="MGIGPLEIGIVLLIVLVIFGPKRLPELGKSLGSGMKSFKDSVSG
                     DDDDKKITTGDTKSDTTVRAEKSEPSA"
     gene            2697..3464
                     /gene="tatC"
                     /locus_tag="VFY44_06050"
     CDS             2697..3464
                     /gene="tatC"
                     /locus_tag="VFY44_06050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX5910037.1"
                     /translation="MARFRPIDHEERLALVDHLGELRSRLIISVIAFAITSGFCWWQN
                     DVILKIINKPLPENIPQPITFGVTEQFTTTLMVSAYAGILLALPVILYQLYAFILPAF
                     SPKERKVATPLLLMVPVLFIAGVVFCYFVVLPGAITFLLDFNSDEFRTEVRARDYYSF
                     TTLTMLAMGLGFQVPIGVLALVKLGVTTPKKLRKSRRYAYLGIVILAAFLPTIDPVTL
                     LLESIPLILLYEGSIWLSAALVKPDAPTTELAGAEST"
     gene            3481..4182
                     /locus_tag="VFY44_06055"
     CDS             3481..4182
                     /locus_tag="VFY44_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910038.1"
                     /translation="MLFDLQGKRRRMVQVTYVTLAILMGGGLVLFGIGGDVQGGIFDA
                     FTGEGGSSNNGNSVIEKRVDSNEKKLKASPQSERLRKSLTRDYYQLATAQADASGKFS
                     DDGKENLRRSAANWQAYLALEPEKPDTSLARLALQVYDPTALNRKAQAQKAAQLIAQE
                     ENDAQAYLNLVQYAALAGDTRTADLAGQKAVDLAPAAQRKQVRAQVKQLKNPQAAQGA
                     QGGQGAAGSGATPQQ"
     gene            4250..4606
                     /locus_tag="VFY44_06060"
     CDS             4250..4606
                     /locus_tag="VFY44_06060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5910039.1"
                     /translation="MNFHIQDEEIDSETHVIQLGGEIDLYTAPEFKERMVELIEGGKN
                     HIVVDLSEATFIDSTTLGVLVGGVKRLRPSGGGLALVCTDQNITKIFEITGLDRVFPI
                     HGTRDEALSSVATGAE"
BASE COUNT          735 a         1671 c         1568 g          670 t
ORIGIN      
        1 cacgccccag aagccgccgc tgtcgctgtt cttccggctc tggttcgacc gcgtcgtgcc
       61 gctgctgggc cgcctggccg gcgactcgga ggcctacacc tacctgccga gctcggtgcg
      121 ccgcttcccc ggccccgagc cgctggcggg ccagctggcg gccgccgggc tggtggacgt
      181 gggctgggtg ctcacggccg gcggcatcat cgccctgcac cacggcaccg tgccggaggc
      241 agggcctccg gcatgagcac cacgccggcg accctggcgt ccgtgctgga cgcgggcggg
      301 ccggcgctgg cccgggcgct ggagcggatc gagacccgcc tcggcgaggt cacgcagggc
      361 cacggcgccg agctggccgg ccacgcctcc ggcacgctgt cggccggcgg caagcggctg
      421 cgccccgtgg tggtcgtcct cagcggcggg gagggcgacg cggtcacctc ggcggcggcc
      481 gccgtggagc tgctgcacat ggccaccctg gtccacgacg acgtgcttga cgccgcccgg
      541 ctgcgccggg gcgttcccac ggtgttcgcc gtcgggggcc gcggggccgc cacggccacg
      601 ggcgacctgc tgttctcgcg cgccttcgcc gagctggcct ccacccgcag cgaggccgcg
      661 gtgcaggccc tgtccaccgc ctcttcggcg ctcgcgcgcg gcgagctgat gcagcgggcc
      721 gacgcctggt cggccaccgt gtcggccgag cgctacctgg agcggtgcga gctcaagacc
      781 gcgagcctct tcgccgcggc ggcccgcctg ggcgccacgc tgggggactc gccggcggcg
      841 gccgacgggc tggccggctt cggccgccgg atcgggctgg cgttccagat cctggacgac
      901 gtgctcgacg tctccggccc ggcggagcgc acgggcaagc accgcggcac cgacctcctg
      961 gacggcacgg tcacgctgcc gctgatcatc gcccgcgaac gcgaccccga gctggccgcg
     1021 atggacctgg cggccgtcgt caccgagccc gcccaggcgg acgccgtgtg cgaccggatc
     1081 gccaggacgg gggccctggg cacggcgcgc gagcaggcgc tgggctacgt ggccgaggcc
     1141 aaggcggcgc tgggcgacat cgacatgccg gccgaccggc tgaacgcgct cggcctggtg
     1201 gccgacggcg tggtggcccg gtacgcgtag gagggcggcg aagccgccct ttcacgagag
     1261 ctagaagtcc tctggaagga ctgcgccgac gtccaaggcc gaggcgaacc gctcgacctc
     1321 ttcggccatg ttggcccgca tcaggtggcg gtagtcggcc atcagggcgt cggtgccggc
     1381 gtccagctct tcgtcgaacg cctcgacggc gctctgggag aacacgccct cgcggaaggt
     1441 ctcgcactcg gggcagcgga tggtcaccga ccagtggtcg ctgcccgcct cctcccaggc
     1501 ggtggggtag gccaggcggg acgcgcactc gatgcagacg tgcaggtcgt ccgccggatc
     1561 caggacgggg cggtcgtgcg ccgccgcctc gccgccgtcg ccgtcgccga agtacaccac
     1621 ctcgatcgtc ttgccgctgg gcaggacgac gcgcttcacg acgtgctccc gatcgctctg
     1681 gtggtgctcc atgggtgcgg tatcggcagg caccccggcg acttgaggat ccggtcgccc
     1741 ggctggactc gtagactgcg gcgccccgtg gttctcagcg atcgcaccat ccgccaggag
     1801 atcgagtccg gccgcatcgt cgtcgacccg ttcgacgatt ccctgatcca gccgtcgagc
     1861 atcgacgtgc gcgtggacag ccgctaccgc gtcttccaca accagcgcca cccctacatc
     1921 gacgtgcgaa agccgatgga cgacctgacc gagctggtcg aggccggtgg cgaggagccc
     1981 ttcatcctgc acccgggcga gttcgtgctg gggcagacgc tggagaaggt cacgctcccc
     2041 aacgacctgg tggctcgcct ggagggcaag agctcgctgg gccgactggg cctgttgatc
     2101 cactccaccg ccggcttcgt ggacagcggc ttctcgggca acctgaccct ggagctgtcc
     2161 aacgtggcca acctgccgat cacgatctac gagggcatgc cgatcggcca gatctcgttc
     2221 atgcgcatgg acgggccggt cgaccgcccg tacggctcgg agtcgaacac gagcaagtac
     2281 cagggccaga ccgagcccac ggccagccgt ttcttcgaga acttcaagga ccgtgactga
     2341 tccgtgaacc cccacccgga cgaagaacta cccactagag tttgactcgc gaccggccca
     2401 acggatagag cgcgagtcgc gcccccgtct taggagctgc acacatggga atcggacccc
     2461 tcgaaatcgg catcgtcctt ctgatcgtcc tcgtcatctt cggaccgaag cgcctgcccg
     2521 agctgggtaa gtccctgggt agcggcatga agtccttcaa ggactcggtg tccggcgacg
     2581 acgacgacaa gaagatcacc accggtgaca cgaagtcgga cacgaccgtg cgcgccgaga
     2641 agtccgagcc ctcggcctag ccggccgccg cacccccggt aggcacgcgg acccccgtgg
     2701 ctcgcttccg acccatagac cacgaggagc ggctcgcgct cgtcgaccac ctcggcgagc
     2761 tgcgctcacg gctgatcatc tcggtcatcg cgttcgcgat cacctccggc ttctgctggt
     2821 ggcagaacga cgtgatcctg aagatcatca acaagcccct gccggagaac atcccgcagc
     2881 ccatcacctt cggcgtcacc gagcagttca ccaccacgct gatggtctcc gcgtacgcgg
     2941 gcatcctgct ggcgctgccg gtgatcctgt accagctcta cgccttcatc ctgcccgcct
     3001 tcagcccgaa ggagcgcaag gtcgccaccc ccctcctgct gatggtgccg gtcctgttca
     3061 tcgccggggt ggtgttctgt tacttcgtcg tgctgccggg agcgatcacg ttcctcctgg
     3121 acttcaactc ggacgagttc cggaccgagg tccgggcccg cgactactac agcttcacga
     3181 ccctcaccat gctggccatg ggtctcggct tccaggtccc gatcggcgtc ctcgcgctcg
     3241 tcaagctcgg cgtgaccaca ccgaagaagc tccgcaagag ccgccgctac gcctacctgg
     3301 gcatcgtgat cctggccgcg ttcctgccga cgatcgaccc ggtcacgctg ctcctggagt
     3361 cgatacccct gatcctgctc tacgagggca gcatctggct gtcggccgcg ctcgtgaagc
     3421 cggacgctcc gaccaccgag ctggccggag cggagagcac ctagataccc tccgcagccg
     3481 atgctcttcg acctgcaggg aaaacggcgc cggatggtgc aggtgacgta cgtgacgctg
     3541 gccatcctga tgggtggcgg gctggtgctg ttcggcatcg gcggcgacgt ccagggcggc
     3601 atcttcgacg cgttcaccgg tgagggaggc agctccaaca acggcaacag cgtgatcgag
     3661 aagcgcgtgg actcgaacga gaagaagctc aaggccagcc cccagagcga gcggctgcgc
     3721 aagtcgctga cgcgcgacta ctaccagctg gccacggccc aggccgacgc cagcggcaag
     3781 ttcagcgacg acggcaagga gaacctgcgc aggtcggccg ccaactggca ggcctacctg
     3841 gcgctggagc ccgagaagcc cgacacgtcg ctcgcgcgcc tggcgctcca ggtctacgac
     3901 ccgaccgcgc tgaaccgcaa ggcccaggcg cagaaggcgg cgcagctgat cgcccaggag
     3961 gagaacgacg cccaggccta cctcaacctg gtgcagtacg ccgcgcttgc cggcgacacg
     4021 cgcaccgctg acctcgccgg ccagaaggcc gtcgacctgg ctccggcggc ccagcgcaag
     4081 caggtgcgcg cgcaggtcaa gcagctgaag aacccccagg ccgcccaggg cgcccagggg
     4141 ggccagggag cggccgggtc gggcgcgacc ccccagcagt aatgcccgac gggctccggc
     4201 cgataaggtc ccgactgggg gccaggggac gatcgagaag aggagcgaag tgaacttcca
     4261 catccaggat gaggagatcg acagcgagac gcacgtcatc cagctcggag gcgagatcga
     4321 tctctacaca gcgcccgagt tcaaggagcg gatggtcgag ctcatcgagg ggggcaagaa
     4381 ccacatcgtg gtcgacctct ccgaggccac cttcatcgac tccaccaccc tcggcgtgct
     4441 ggtcggcggc gtgaagcgcc tgcgaccctc cggcggcggc ctcgccctgg tctgcaccga
     4501 ccagaacatc accaagatct tcgagatcac cggcctggat cgggtcttcc cgatccacgg
     4561 aacacgcgac gaggcgctct cgtcggtggc caccggcgcg gaatagaatc tcgccccttc
     4621 ggggccgtta gctcaactgg taga
//
LOCUS       DASUPW010000266         4989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_200675, whole genome shotgun sequence.
ACCESSION   DASUPW010000266 DASUPW010000000
VERSION     DASUPW010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200675"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1326)
                     /locus_tag="VFY44_06065"
     CDS             complement(115..1326)
                     /locus_tag="VFY44_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5910040.1"
                     /translation="MINLEIPSKFKPLVGQAHQVATEMFRPISRKYDLAEHEYPKELD
                     ALAALLDGMDDSSGSSGAGASGASRQSNGDGGGNRNGSNLSTVLGIMELCWGDVGLLL
                     SMPRQGLGNSAIAAVADAEQAERYDGLWAGMAITEPEAGSDSAAIRTTAELDGDEYVL
                     NGEKIYVTSGERADVLVVWATLDRDAGRAAIKSFVVYRDNPGLKLDRLEHKLGIRASD
                     TAAFILEDCRVPKEDLLGSPEIDMQKSFGGAMKTFDNTRPLVAGMAVGVARACIEETE
                     KHLEAAGVTVDYDRPAATQSAAAAELLALQADFESARLLTMQAAWMADNSKPNSVEAS
                     LSKAKAGRSATDIALRCVKLCGTLGLTENELLEKWARDVKILDLFEGTQQIQQLIIAR
                     QLLGKSSAELK"
     gene            complement(1326..2642)
                     /locus_tag="VFY44_06070"
     CDS             complement(1326..2642)
                     /locus_tag="VFY44_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5910041.1"
                     /translation="MSVADRSMGLGLRGLRALAGLEVIDRAGLRKPVERLIYEGSKNG
                     FSAATAAGRTFTAATKLGKPARQAKPSAPKLFDLEPDDEQKMLQEAFGDFGSEKLRPI
                     AQDADSACKAPDDVLAQANELGASMLGVPEELGGVMTERSAVTSVLVAEKLAHADMGL
                     AAAILAPGAVSTAISLWGDAEQQSKYLPPFTEDDAPAAALAIQEPRVLFDPLELQTVA
                     TRSPDGFTLSGAKSLVLRAADAELFVVAARIEGEGPALFIIESGTQGLSVEADPAMGV
                     RAAATGTLRLDDVAVGPDALLGGADPAVYSECVQRSRIAWAALAIGTGRAVLDYVIPY
                     VNERQAFGEPISNRQAVAFTVSDIAIELEGMKLTTLRAASRADLGKSFAKEATIARQL
                     VTDRGMQIGSSGVQLLGGHGYVKEYPVERWYRDLRSAGVMEGALLV"
     gene            complement(2639..3493)
                     /locus_tag="VFY44_06075"
     CDS             complement(2639..3493)
                     /locus_tag="VFY44_06075"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX5910042.1"
                     /translation="MVGTYARAAAPLIPGASRLPFVGGGGGPMPDTVLRLDGVKVDPG
                     ALARYARVCGFRLAETLPATYPHILAFPLHMQLMTAGDFPFPAVGLVHIENRIVQHRP
                     IAVGEPLDLTVRATQPEEHPKGRQFTLVTEARVGRELVWEDFSTNLRRGKGSGEEKKG
                     GSRKKPPPPASDWKLDGGLGRRYAAASGDRNPIHMHDLSAKLFGFPRAIAHGMWTKAR
                     SLAALEGRLPDAYEVDVAFKKPILLPATVTFGSESSGTTTRFTVRSRKDVPHLDGTVK
                     AVKTRRKS"
     gene            complement(3495..4898)
                     /locus_tag="VFY44_06080"
     CDS             complement(3495..4898)
                     /locus_tag="VFY44_06080"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF006110.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEX5910043.1"
                     /translation="MADLYTQIVNAPVANTLASTLGLPVPTPLERYSPGDPVLKGSAL
                     VGAAPGGRLGGPVVAVLAQAGVNAATEMDEELRDAAAAAGLDAPLWNSAAPSGDKFKA
                     LVFDASGITTSEQLSELHRFFHPAIRAVAPNGRVVVLGTPPSDTGSPSEHTAQRALEG
                     FTRSLGKEMRGGGTVQLVYVEQGAEQQLASTLTFLLSPKSAYVSGQVVRIGKADLPPE
                     VSWDKPLDGKVALVTGASRGIGAAIAAVLARDGAHVVGLDIPALGDDLRTVTSELGGS
                     ALEVDVTDDDAPKLIADHLKAEHGGVDVVVHNAGVTRDKTIAGMDEGRWNQVIDINLS
                     SQERIDNQLLAAEVLNDGARIVTVSSMSGIAGNNGQTNYATSKAGVIGMVDSMAPVFA
                     NAGATINAVAPGFIETQMTQAMPVPVREAGRRMSSLSQGGLPVDVAETIAWFASPAST
                     GLNGNTVRVCGQNLLGA"
BASE COUNT          641 a         1827 c         1766 g          755 t
ORIGIN      
        1 ggtccatccc gtcctgccac tgcggcgcgg acgcggtgtc ctcgaccacg gcccacaccc
       61 ggtccagcgg cgcgtcgatg acctcgctgg cggttccgtt caggttcccc atggctactt
      121 cagctccgcg gagctcttgc ccagcagctg ccgggcgatg atcagctgct ggatctgctg
      181 ggtgccctcg aacaggtcca ggatcttcac gtcgcgggcc cacttctcca gcagctcgtt
      241 ctcggtcagg ccgagcgtgc cgcacagctt cacgcagcgc agcgcgatgt cggtggccga
      301 gcggccggcc ttggccttgg acagcgaggc ctcgaccgag ttgggcttgg agttgtcggc
      361 catccacgcc gcctgcatgg tcagcagccg cgccgactcg aagtcggcct gcagcgccag
      421 tagctcggcc gcggcggccg actgggtggc ggcggggcgg tcgtagtcca cggtcacgcc
      481 cgcggcctcg aggtgcttct cggtctcctc gatgcacgcc cgggccactc cgacggccat
      541 gcccgccacc agcgggcggg tgttgtcgaa cgtcttcatg gcgccgccga acgacttctg
      601 catgtcgatc tccggcgagc cgagcaggtc ctccttgggc acgcggcagt cctcgaggat
      661 gaaggccgcg gtgtccgagg cacggatgcc cagcttgtgc tccaggcggt ccagcttcag
      721 gcccgggttg tcgcggtaga ccacgaacga cttgatggcc gcgcggcccg cgtcgcggtc
      781 cagcgtggcc cagaccacga gcacgtcggc ccgctcgccc gaggtcacgt agatcttctc
      841 gccgttgagc acgtactcgt cgccgtccag ctccgcggtg gtgcggatgg cggccgagtc
      901 cgagcccgcc tcgggctcgg tgatggccat gccggcccag agcccgtcgt agcgctcggc
      961 ctgctcggcg tccgccaccg cggcgatggc cgagttgccc aggccctgcc gcggcatcga
     1021 cagcagcagg ccgacgtcac cccagcacag ctccatgatg cccagcaccg tggagaggtt
     1081 ggagccgttg cggttgccgc cgccgtcgcc gttggactgc cggctggcgc cgctggcccc
     1141 ggcgcccgag gagccgctgc tgtcgtccat gccgtccagc agggcggcca gcgcgtccag
     1201 ctccttgggg tactcgtgct cggccaggtc gtacttgcgc gagatcggcc ggaacatctc
     1261 ggtcgccacc tggtgggcct ggcccaccag cggcttgaac ttggatggga tctccaggtt
     1321 gatcatcaga cgagcagagc tccttccatc acgccggcgg accgcaggtc gcggtaccag
     1381 cgctcgacgg ggtattcctt gacgtagccg tggccgccca gcagctgcac gccgctcgag
     1441 ccgatctgca tgccgcggtc ggtcaccagc tggcgggcga tcgtggcctc cttggcgaag
     1501 ctcttgccca ggtcggcccg cgacgcggcc cgcagggtgg tcagcttcat gccctccagc
     1561 tcgatcgcga tgtccgagac ggtgaacgcc accgcctggc ggttggagat cggctcgccg
     1621 aacgcctggc gctcgttgac gtaggggatc acgtagtcca ggaccgcgcg gccggtgccg
     1681 atggccagcg ccgcccaggc gatccgcgag cgctgcacgc actcggagta cacggccggg
     1741 tcggctccgc ccagcagggc gtcggggccc acggccacgt cgtccagcct cagcgtgccg
     1801 gtggccgcgg cgcggacgcc catggccggg tccgcctcga cggacaggcc ctgggtgccc
     1861 gactcgatga tgaacagcgc ggggccctca ccctcgattc gcgccgccac cacgaacagc
     1921 tcggcgtcgg cggcgcgcag caccagcgac ttggcgcccg agagggtgaa gccgtccggg
     1981 ctgcgggtgg ccacggtctg gagctccagc gggtcgaaca gcacccgtgg ctcctggatg
     2041 gccagcgccg ccgcgggcgc gtcgtcctcg gtgaacggcg gcaggtactt ggactgctgc
     2101 tcggcgtcgc cccagaggga gatcgccgtg ctcacggcac ccggcgccag gatggcggcg
     2161 gccaggccca tgtcggcgtg ggccagcttc tcggccacca gcaccgaggt cacggccgag
     2221 cgctcggtca tcacgccgcc cagctcctcg ggcacgccca gcatcgacgc gcccagctcg
     2281 ttggcctggg ccagcacgtc gtccggcgcc ttgcaggcgg agtcggcgtc ctgggcgatc
     2341 ggccgcagct tctcggagcc gaagtccccg aacgcctcct ggagcatctt ctgctcgtcg
     2401 tcgggctcca ggtcgaacag cttcggcgcc gacggcttgg cctgccgcgc cggcttgccg
     2461 agcttggtgg cggcggtgaa ggtccgcccg gcggcggtgg ccgcgctgaa cccgttcttg
     2521 gacccctcgt agatcagccg ctccacgggc tttcgcagcc ccgcgcggtc gatcacctcg
     2581 agtccggcca gggcacgcag gccccggagg ccgaggccca tgctgcgatc ggcgacgctc
     2641 acgacttcct cctcgtcttg acggctttca cggtcccgtc caggtgcggc acgtccttgc
     2701 gcgagcgcac cgtgaagcgg gtggtggtgc cggacgactc ggacccgaag gtcacggtcg
     2761 ccggcaacag gatcggcttc ttgaaggcca cgtcgacctc gtaggcgtcg ggcaggcggc
     2821 cctccagggc ggccagcgac cgggccttgg tccacatgcc gtgcgcgatc gcgcgcggga
     2881 acccgaacag cttggccgac aggtcgtgca tgtggatcgg gttgcggtcg cccgaggcgg
     2941 ccgcgtagcg gcggccgagg ccgccgtcca gcttccagtc ggaggcgggg ggaggcggct
     3001 tcttgcgcga gccgcccttc ttctcctcgc ccgagccctt gccgcggcgc aggttggtgg
     3061 agaagtcctc ccacaccagc tcccggccca cgcgggcctc ggtgaccagc gtgaactggc
     3121 gccccttggg gtgctcttcc ggctgggtgg cgcggaccgt caggtccagc ggctcgccca
     3181 ccgcgatcgg ccggtgctgg acgatccggt tctcgatgtg caccagcccc accgcgggga
     3241 acgggaagtc gcccgcggtc atgagctgca tgtgcagcgg gaacgccagg atgtgggggt
     3301 acgtcgcggg cagcgtctcc gcaaggcgga agccgcacac gcgtgcgtac cgggccagcg
     3361 cgccgggatc gaccttcacg ccgtccaggc gcagcacggt gtcgggcatg ggcccaccgc
     3421 caccgcccac gaagggcagt cgcgatgcgc cggggatcag gggcgcggcc gcgcgcgcgt
     3481 aggtgccgac cacgtcaggc ccccagcagg ttctggccgc acacgcggac cgtgttgccg
     3541 ttcaggccgg tcgaggcggg ggaggcgaac caggcgatcg tctcggccac gtccaccggc
     3601 aacccgccct gcgacaggct ggacatgcgc cggccggcct cacgcaccgg caccggcatc
     3661 gcctgcgtca tctgggtctc gatgaagccg ggggcgaccg cgttgatggt cgcgccggcg
     3721 ttcgcgaaca cgggggccat cgagtcgacc atcccgatca cgcccgcctt ggacgtggcg
     3781 tagttggtct ggccgttgtt gccggcgatg ccgctcatcg aggacacggt cacgatccgc
     3841 gccccgtcgt tgagcacctc ggccgccagc agctggttgt cgatccgctc ctggctggac
     3901 aggttgatgt cgatcacctg gttccagcgg ccctcgtcca tgcccgcgat cgtcttgtcg
     3961 cgggtcacgc cggcgttgtg gaccacgacg tccacgccgc cgtgctcggc cttgaggtgg
     4021 tcggcgatca gcttcggcgc gtcgtcgtcg gtcacgtcga cctccagcgc cgagccgccc
     4081 agctcggacg tgaccgtgcg caggtcgtcg cccagcgccg ggatgtccag gccgaccacg
     4141 tgcgcgccgt cgcgggccag caccgcggcg atggccgcgc cgatgccgcg cgacgcgccg
     4201 gtgaccagcg ccaccttgcc gtccagcggc ttgtcccagc tgacctccgg cggcaggtcg
     4261 gccttgccga tgcgcacgac ctggcccgac acgtacgccg acttgggcga cagcaggaac
     4321 gtcagcgtcg aggccagctg ctgctcggcg ccctgctcga cgtacaccag ctgcacggtg
     4381 cctccgccgc gcatctcctt gcccagcgag cgggtgaagc cctccagcgc gcgttgcgcc
     4441 gtgtgctcgg acggcgagcc ggtgtcggac ggcggggtgc ccagcaccac cacgcgcccg
     4501 ttgggcgcca ccgcgcggat ggcggggtgg aagaagcggt gcagctcgga gagctgctcg
     4561 ctggtggtga tgcccgaggc gtcgaacacc agcgccttga acttgtcgcc cgagggcgcg
     4621 gcggagttcc acagcggcgc gtccagcccg gccgcggccg cggcgtcgcg cagttcctcg
     4681 tccatctcgg tcgcggcgtt cacgccggcc tgcgccagca ccgccacgac gggaccgccc
     4741 agccgtccgc ccggggccgc gcccaccagg gcgctgccct tgagcacggg gtcgccgggc
     4801 gagtaccgct ccagcggcgt cggcaccggc agcccgagcg tgctggccag cgtgttggct
     4861 accggcgcgt tgacgatctg tgtgtagagg tctgccatca gctctgctcc ttctcgagaa
     4921 tggccaccac gccctggccg cccgcggcgc agatcgagat cagcccgcgg ccgcctccgc
     4981 gctcgttca
//
LOCUS       DASUPW010000267         2233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_893475, whole genome shotgun sequence.
ACCESSION   DASUPW010000267 DASUPW010000000
VERSION     DASUPW010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893475"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="VFY44_06085"
     CDS             <1..1020
                     /locus_tag="VFY44_06085"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5910044.1"
                     /translation="AGGSIGLLLGGVLTQSINWHWIFFVNIPIAIGTAVAAQRLLKDD
                     QGIGFSKGADTPGAVLITSSLMLGVYTIVKPGAELGFGAAQTLILGAVSVALLVGFVV
                     REAKAENPLVPLRMFRSRNVSGANAIAALTVAGMFGMFFIGALYLEQVLGYSALEIGL
                     AFLPCTVVMGGLSLRYTEALITRFGASRLLAPGMALIAAGLALFALAPVDANYVQHVL
                     PSTALLGLGAGISMPALMTVAMSGATPEDAGLASGLVNTTTQMGAALGLAVLATVSSS
                     RTDSLAAAGESARQALTGGFHVAFLVGAALVAVGIVVALVVTREPKPAAETVHASPEL
                     VSEAA"
     gene            1017..1670
                     /locus_tag="VFY44_06090"
     CDS             1017..1670
                     /locus_tag="VFY44_06090"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5910045.1"
                     /translation="MTFVLVHGAADRAASWDEVAAALRGRGHDVVAVDLPCEDDGAGF
                     GDYADVVAEAVGDRGDLVLVGHSLGGYTAALLAERLPTRMLVLVTAMVPRPGETPMEF
                     WANTGHRFGSDDVVETYLADATPEQAERALALGRDQSGTPMGEPFPLEAWPDVPTRYL
                     ACTRDNFNPVEWTRAMVRDRLGIEADEIDAGHCPYITRADELAQRLHDYARKAAPPA"
     gene            1680..>2233
                     /locus_tag="VFY44_06095"
     CDS             1680..>2233
                     /locus_tag="VFY44_06095"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HEX5910046.1"
                     /translation="MTLVDDLLAQVHDRWMDDRSGEVLHGIPALENVDPDTFGICLAT
                     ADGYVYEQGDTDLPFCIQSISKAFTYGMALMDNGTKQVDAKIDVEPSGDLFNEISLHP
                     VTHRPRNPMINAGAIAASSMVRGKDVHEQVERVQRTYSDYAGAELKIDEDIYASMQES
                     GHRNRAISHMLREFGILECSPDDAL"
BASE COUNT          305 a          793 c          807 g          328 t
ORIGIN      
        1 gccggcggct cgatcggcct gttgctgggc ggcgtcctga cccagtcgat caactggcac
       61 tggatcttct tcgtgaacat cccgatcgcc atcggcaccg cggtggccgc gcagcggctg
      121 ctcaaggatg accagggcat cggcttctcc aagggcgccg acaccccggg tgcggtgctg
      181 atcaccagct ccctgatgct gggcgtctac accatcgtca agccgggagc ggagctgggc
      241 ttcggcgccg cgcagacgct gatcctgggc gccgtgtcgg tcgccctcct ggtgggcttc
      301 gtggtccgtg aggccaaggc ggagaacccg ctggtgccgc tgcggatgtt ccgctcgcgc
      361 aacgtgtcgg gtgccaacgc catcgcggcg ctgaccgtgg ccggcatgtt cggcatgttc
      421 ttcatcggcg ccctgtacct ggagcaggtc ctgggctact ccgcgctgga gatcggcctg
      481 gccttcctgc cctgcaccgt ggtgatgggc gggctgagcc tgcgctacac cgaggcgctg
      541 atcacccgct tcggagccag ccggctgctg gccccgggca tggccctgat cgccgccggg
      601 ctggcgctgt tcgcgctggc gccggtcgat gcgaactacg tgcagcacgt cctgcccagc
      661 accgcgctgc tggggctggg ggccgggatc tcgatgcccg cgctgatgac ggtggccatg
      721 tccggcgcca cccccgagga cgcggggctg gcctccgggc tggtgaacac cacgacgcag
      781 atgggcgccg cgctggggct ggcggtgctg gccaccgtgt cgtcctcgcg caccgacagc
      841 ctggccgcgg ccggcgagtc ggcccgccag gcgctcaccg gcgggttcca cgtggccttc
      901 ctcgtgggcg ccgcgctggt ggcggtcggg atcgtggtgg cgctggtggt cacccgcgag
      961 cccaagccgg ccgccgagac ggttcacgcc tctcccgagc tggtctccga ggcggcatga
     1021 cgttcgtcct ggtccacggg gcggccgacc gggccgccag ctgggacgag gtggccgcgg
     1081 ccctcagggg tcgcggccac gacgtcgtcg cggtcgattt gccgtgcgag gacgacggcg
     1141 ccggcttcgg cgactacgcc gacgtggtgg ccgaggccgt cggggaccgc ggggacctgg
     1201 tcctggtcgg ccactcgctg ggcggctaca ccgcggcgct gctggccgag cgcctgccca
     1261 cccgcatgct ggtgctggtc accgccatgg tcccgcgccc gggcgagacc ccgatggagt
     1321 tctgggcgaa caccggccac cgcttcggca gcgacgacgt cgtggagacc tacctggccg
     1381 acgccacgcc cgagcaggcc gagcgcgccc tggcgctggg ccgcgaccag tcgggcacgc
     1441 cgatgggcga gccgttcccg ctggaggcgt ggccggacgt gcccacgcgc tatctggcgt
     1501 gcacgcggga caacttcaac ccggtcgagt ggacgcgcgc gatggtccgc gaccggctgg
     1561 gcatcgaggc cgacgagatc gacgccggcc actgccccta catcacgcgg gccgacgagc
     1621 tggcgcagcg cctgcacgac tacgcgcgga aggcggcgcc cccggcctag tatcgggctg
     1681 tgacgctcgt cgacgacctc ctggcccagg tccacgaccg ctggatggat gaccgctcgg
     1741 gcgaggtgct gcacgggatc ccggcgctcg agaacgtcga cccggacacg ttcggcatct
     1801 gcctggccac cgccgacggg tacgtctacg agcaggggga caccgacctg ccgttctgca
     1861 tccagtcgat ctcgaaggcc ttcacctacg ggatggcgct catggacaac ggcaccaagc
     1921 aggtggacgc caagatcgac gtggagccct cgggcgacct gttcaacgag atcagcctgc
     1981 acccggtcac ccaccggccg cgcaacccga tgatcaacgc gggggcgatc gcggcgtcga
     2041 gcatggtcag gggcaaggac gtgcacgagc aggtcgagcg cgtgcagcgc acctactccg
     2101 actacgccgg cgccgagctg aagatcgacg aggacatcta cgcgtcgatg caggagtccg
     2161 ggcaccgcaa ccgcgccatc agccacatgc tgcgcgagtt cgggatcctg gagtgctcgc
     2221 ccgacgacgc gct
//
LOCUS       DASUPW010000268         9832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1724727, whole genome shotgun sequence.
ACCESSION   DASUPW010000268 DASUPW010000000
VERSION     DASUPW010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9832
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724727"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="VFY44_06100"
     CDS             complement(<1..810)
                     /locus_tag="VFY44_06100"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEX5910047.1"
                     /translation="MSPLKLWFIAARPRTLPAAVAPVLVGTALAITADEFGAVAFVAA
                     LVGSVFLQIGTNLANDYSDARRGADTEDRLGPVRVTAGGLMPPRRVLAGTYFAFGVAC
                     VAGVVLTVIAGPVMLAVGAASIVAGVLYTGGPRPYGYSGLGEVFVFVFFGVVAVVFTF
                     YAQAESYPWEAFALSVPVGLLASAILVVNNVRDVDTDRRAGKRTLAVKLGRERARRLF
                     DAMLALSYVMCVVIPAAGGLTWWTLLPLLSIPLALPLRKTVATRTDGPALNG"
     gene            complement(810..1676)
                     /gene="menB"
                     /locus_tag="VFY44_06105"
     CDS             complement(810..1676)
                     /gene="menB"
                     /locus_tag="VFY44_06105"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016079909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HEX5910048.1"
                     /translation="MADSWTDTQAARTIAPGADWQPQGEYEDIRYETADGIAKITIAR
                     PEVRNAFRPQTLVEVDRALQLARNDGSVGVIVLTGEGPLAFCSGGDQNVRGDTGYLAE
                     GEEVGRFHVTDLQVAIRRCPKPVVAMVAGYAIGGGHVLHVVCDLTIAADNARFGQTGP
                     SVGSFDGGFGASILSDLVGPKKAKEIWFLCRQYGAEEAESMGLVNTVVPLEDLEVETV
                     AWARRMLELSPFALRLLKASFHASEDGYSGIQQLAHDANLLFYGSDEAKEGREAYKAK
                     RKPDFGQFPKRP"
     gene            1654..2988
                     /locus_tag="VFY44_06110"
     CDS             1654..2988
                     /locus_tag="VFY44_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5910049.1"
                     /translation="MSVQESAIDGRTIVRLDEWLDLAARARPDHPAVESASGTLSYGE
                     LDALATVTARRLAAQGVGEGDRVATTLAPGADFAALLHALPRLGAALVPLNTRLPAAA
                     QRRQRDLSGAMMVIDEPLADGFEAEPVLRTRLDAAAVHTMLFTSGTSGEPKPVELTVG
                     NQEASAAGSQAVFGYGPGDRWLCPLPLFHIGGLAILIRCARAGATAVLADRVDPAAME
                     GVTLASVVATQLRRLRDAGLTAAPGLRALVLGGGPVPPELMEWAGGHELPVRATYGMT
                     ETCSQVAVTAPWERTAAPVPGAELVTGPRSEILVRGPMVAPGATGEGGWLHTGDVGRI
                     CRDGTLAVEGRIKDLIVTGGENVAPAAVEAALTAHPAVADAAVAGVPDPEWGEAVTAY
                     VVERRPVSDYELLSFCRERLAGYQVPKAVVRVAELPRNAAGKLQRDLLGSAP"
     gene            3002..3991
                     /gene="ilvC"
                     /locus_tag="VFY44_06115"
     CDS             3002..3991
                     /gene="ilvC"
                     /locus_tag="VFY44_06115"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF004017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX5910050.1"
                     /translation="MIYDDDADLSALDGKTVAILGYGSQGHAHALNLKDSGVDVVVGL
                     REDSSSVKKAEADGLEVLSIADAASKGDVVMILLPDEKQGQLWREEIADGIAPGNTLM
                     FAHGFAILYGEIEPGPGVDVGLAAPKGPGHLVRRQYEKQEGIPGLVAIHADESGNARD
                     VILAYAKGIGCTRAGVIETTFKEETETDLFGEQAVLCGGVSELVQAGFETLVDAGYSP
                     QMAYFECLHEVKLIVDLMYEKGLAGMRYSISNTAEYGDYTRGKRVITDETRESMKKIL
                     GEIQSGEFAREWIAENNAGQENFLRMRQEQADTQIEREGKELRSKMDWIDSEF"
     gene            3994..4578
                     /locus_tag="VFY44_06120"
     CDS             3994..4578
                     /locus_tag="VFY44_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910051.1"
                     /translation="MAADSPGHGGFLPPEPTGPEPDLGRRPEPAAAPPPAPASGYEPP
                     AHAQPGWQPPAQPQGWQHPQAAAPDNGPAVLGFSLSISSLGLLIISAGLSTLLSVGLA
                     IPGMVQSRKGTQRVREGVTDRNAGLANAGWILGIVALVLSALATIFWIVIAIVVATDD
                     EARREFEREFDRQYDQSSMSALIVVARLVGAALL"
     gene            4575..6080
                     /locus_tag="VFY44_06125"
     CDS             4575..6080
                     /locus_tag="VFY44_06125"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5910052.1"
                     /translation="MRAAAVALVAAMALVPAGAAGPAEAAKRPTRPVVMVVFDELPLT
                     SLLDGRGRIDRVRYPNFAALAGDATWYANATTVSDATKLAIPSILDGRLPKVGVPATH
                     RGHPRNVFTLLRGNGYRLKVQEEATSLCPYRGCRRRHGAHYFLSRDRLGRFERWIAGI
                     GRSRRPTLYYKHALLPHVPWVFTPQLRRYDRSVLGPIKGLNSSERSVFDRTLVRQSWQ
                     RHLLQVGAVDTLLGRIVARMKATGVYDRASVVIMADHGVSFHVGATDRRTILPFNAFD
                     VAPIPLFIKAPGQRRGRIDRSMMRTYDVLPTVAGQIGLRLPRGLSGRSATSNRVRRRG
                     RVKVMSRAPLARITFSRGRLAQGRRAAVRRKLALFGSGTRSLYLFGANSQLLGRQVAS
                     LRVRRRGAVRATLNGARDYRRIYRRSRFIPAHVTGRITGRRRGARRDIAVAVNGRIWA
                     VTRSVRLRGSRSEYYSALLNPDKLVVGRNRNAIAVYEVTRSRGKYALRRLR"
     regulatory      6086..6172
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            6219..7325
                     /gene="serC"
                     /locus_tag="VFY44_06130"
     CDS             6219..7325
                     /gene="serC"
                     /locus_tag="VFY44_06130"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:TIGR01366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEX5910053.1"
                     /translation="MEIPQDLKPADGRFGCGPSKVRPEQLASLAADGAAIMGTSHRQK
                     PVKSLVGRVRQGLAQLLGLPEGYEVLLGNGGTTALWDALAFGIVRERALHLTYGEFSS
                     KFAKVTTGAPFLADPVVVESDPGTAPELSSDDSCDVVTWAHNETSTGVMLGVERPAGD
                     ALVLVDATSGAGGLPLHAQNADVYYFAPQKSFAADGGIWFAACSPAAIERIEEIGASD
                     RWIPDFLSLTTALENSRKDQTYNTPALATLWLLAEQVDWMNAGGGLQEFCVARTQESS
                     SHLYSWAGSSEFASPFVAQQANRSLVVGTIDFDDSVDAAAVAATLRANGIVDTEPYRK
                     LGRNQLRIGMFPAVEPDDVRALTACIDYVVEAQS"
     gene            7322..8920
                     /gene="serA"
                     /locus_tag="VFY44_06135"
     CDS             7322..8920
                     /gene="serA"
                     /locus_tag="VFY44_06135"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX5910054.1"
                     /translation="MTKVLVKEKIGDSGVQLLRDAGFDVELGADWEDGELERRIGEFD
                     GILIRSATKLTADLIEKADNLKAVGRAGVGVDNVDVDAATKRGIVVANAPQSNVVTAA
                     EHTMALLTAMARNVPQAHQSLVEGRWDRSKYSGVELYDKTLGIMGFGRIGQLVAERAR
                     AFGMRVLAFDAYVAEERFRELGAERADSSDQVYAEADFITVHLPKTPETENWLDAEAF
                     AKMKDGVRILNVARGPLVVDADLEAALESGKVAGAALDVFRSEPITDHPLFGHPKVIV
                     TPHLGASTAEATDRAGFQAAEQVVAALTGGVVTTAVNVPAVAGEDLEALGPYLPLVSD
                     LGRIGTALAEGTSIDGIDVEYLGRIAERDTRLLTVQVIKGALMGRTEEQVNDVNAPSL
                     AQERGIDVRETRSTSARDFSDLVRVTVRSGDEQTRVVGTVFGRRARPHLLEAWGARFN
                     VQLEDSLAIFRYRDQPGRIGRVGTVLGDAGVNVNSAAVGHHGEDEEPEEAVMIVTTDA
                     PVPQDVVDEIVSEDGFVAGRTITL"
     gene            8938..9327
                     /locus_tag="VFY44_06140"
     CDS             8938..9327
                     /locus_tag="VFY44_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HEX5910055.1"
                     /translation="MAWQPPPVQYQQRAPQANNDLAVAALCMSLASLGVLVVGTPFTF
                     GFSLLLSGPLAIGGLVCGVIARQKADRGEAGGRSMAQAAFVLGIVSLVLHVVAVILGV
                     ALIAVLLESIDDFDVPEPDQGPEADPA"
     gene            9480..>9832
                     /locus_tag="VFY44_06145"
     CDS             9480..>9832
                     /locus_tag="VFY44_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910056.1"
                     /translation="MAEQTSTRSDEDRVLIFDTTLRDGEQSPGISLNKSEKLEIAHQL
                     ARLGVDVIEAGFPIASPGDFEAVQAIAREVQGPIISGLARAGAGDIDAAWNAVRESDR
                     PRIHTFISTSDIHIE"
BASE COUNT         1390 a         3590 c         3545 g         1307 t
ORIGIN      
        1 gccgttcagc gccggcccgt cggtgcgggt ggccacggtc ttgcgcagcg gcagggccag
       61 cgggatcgac agcagcggca acaacgtcca ccaggtgagc ccgcctgcgg ccgggatcac
      121 gacgcacatg acgtaggaga gcgccagcat ggcgtcgaac aggcgccgcg cccgctcccg
      181 gcccagcttc acggccagcg tgcgcttgcc tgcgcggcgg tcggtgtcca cgtcgcggac
      241 gttgttgacc accaggatcg ccgacgccag caggccgacg ggcaccgaca gcgcgaacgc
      301 ctcccacggg taggactcgg cctgggcgta gaaggtgaag accacggcga ccacgccgaa
      361 gaacacgaac acgaacacct cgccgaggcc cgagtagccg tacgggcggg ggccgccggt
      421 gtacagcacg ccggccacga tcgacgccgc gccgacggcc agcatgaccg ggccggcgat
      481 cacggtgagc acgaccccgg ccacgcaggc caccccgaac gcgaagtagg tgccggccag
      541 cacacgcctg ggcggcatca ggccgccggc ggtcacgcgc accggcccca gccggtcctc
      601 ggtgtcggcg ccgcggcggg catcggagta gtcgttggcc aggttggtgc cgatctgcag
      661 gaagacgctg cccacgagcg cggccacgaa cgcgacggcg ccgaactcgt cggcggtgat
      721 ggccagggcg gtgcccacca gcacgggggc gaccgcggcg ggcagcgtcc gcggccgcgc
      781 cgcgatgaac cagagcttca gcgggctcat cagggccgct tggggaactg gccgaagtcg
      841 ggctttcgct tggccttgta cgcctcgcgc ccctccttgg cctcgtcgct gccgtagaac
      901 aggaggttgg cgtcgtgggc cagctgctgg atgcccgagt agccgtcctc ggaggcgtgg
      961 aagctggcct tgagcaggcg cagcgcgaag ggggagagct cgagcatccg ccgcgcccag
     1021 gccacggtct cgacctccag gtcctcgagc gggaccaccg tgttcaccag gcccatcgac
     1081 tcggcctcct cggcgccgta ctggcgacac aggaaccaga tctccttggc cttcttcggg
     1141 ccgaccaggt cggacaggat gctggcgccg aagccgccgt cgaacgaccc cacgctgggg
     1201 ccggtctggc cgaagcgggc gttgtcggcc gcgatggtca ggtcgcacac cacgtgcagc
     1261 acgtggccgc cgccgatggc gtagccggcc accatggcca cgaccggctt ggggcaccgg
     1321 cggatggcca cctgcaggtc ggtcacgtgg aagcggccga cctcctcgcc ctcggcgagg
     1381 tagccggtgt cgccgcgcac gttctggtcg ccgcccgagc agaaggccag cgggccctcg
     1441 ccggtcagga cgatcacgcc caccgagccg tcgttgcggg ccagctggag cgcccggtcg
     1501 acctcgacca gggtctgggg ccggaaggcg ttgcgcacct cgggccgggc gatcgtgatc
     1561 ttggcgatcc cgtccgcggt ctcgtagcgg atgtcctcgt actcgccctg cggctgccag
     1621 tcggcgccgg gggcgatcgt gcgggcggct tgggtgtctg tccaggagtc ggccatcgac
     1681 ggccgtacga tagttcgcct ggacgagtgg cttgacctcg cggcgcgcgc ccggcccgac
     1741 caccctgcgg tcgagtccgc ctccggcacc ctcagctacg gcgagctgga cgccctggcc
     1801 accgtgaccg cgcggcgcct ggccgcccag ggcgtgggtg agggcgaccg ggtggccacg
     1861 acgctggcgc cgggggccga cttcgcggcg ctactgcacg cattgccccg cctgggcgcc
     1921 gcgctggtgc cgctgaacac gcgcctgccc gccgccgcgc agcgccgcca gcgcgacctg
     1981 tcgggcgcga tgatggtgat cgacgagccg ctggcggacg ggttcgaggc cgagcccgtc
     2041 ctgcgcaccc gtctcgatgc cgccgccgtg cacaccatgc tgttcacctc cggcacgtcc
     2101 ggggagccca agccggtcga gttgaccgtg ggcaaccagg aggcatcggc cgcgggctca
     2161 caggccgtgt tcggctacgg gcccggcgac cgctggctgt gcccgctgcc gctgttccac
     2221 atcggtgggc tggccatcct gatccggtgt gcgcgcgcgg gcgcgaccgc cgtgctggcc
     2281 gaccgcgtcg acccggcggc gatggagggc gtgacgctgg cctcggtcgt ggccacgcag
     2341 ctgcgccgcc tgcgcgacgc cgggttgacc gcggcgcccg gcctgcgtgc cctggtcctg
     2401 ggcggcggcc cggtgcctcc cgagctgatg gagtgggccg gcggccacga gctgcccgtc
     2461 cgggccacct acggcatgac cgagacctgc tcccaggtgg ccgtgaccgc gccgtgggag
     2521 cgcaccgcgg cgcccgtccc cggcgccgag ctggtcaccg gcccccgcag cgagatcctg
     2581 gtccgcggcc cgatggtggc gcccggggcg acgggcgagg gcggctggct gcacaccggc
     2641 gacgtgggcc gcatctgccg cgacggcacg ctggcggtcg agggccggat caaggacctg
     2701 atcgtgaccg gcggcgagaa cgtggccccg gcggccgtgg aggccgccct gaccgcccat
     2761 ccggcggtgg cggacgccgc ggtggccggc gtgccggatc ccgagtgggg cgaggccgtg
     2821 accgcctacg tggtcgagcg ccgcccggtg tccgactacg agctgctgtc cttctgccgc
     2881 gagcggctgg ccggctacca ggtgcccaag gccgtggtgc gcgtcgccga gctgccgcgc
     2941 aacgccgcgg gcaagctcca gcgcgacctg ttgggatccg ccccgtagta gcgtcccggc
     3001 cgtgatctac gacgacgatg cagacctcag cgcactcgac ggcaagaccg tggccatcct
     3061 gggctacgga tcgcagggcc acgcccacgc cctgaacctc aaggactccg gcgtcgacgt
     3121 ggtcgtaggc ctgcgcgagg actcgtcctc ggtcaagaag gccgaggccg acggcctcga
     3181 ggtgctgtcc atcgccgacg ccgccagcaa gggcgacgtg gtcatgatcc tgctgcccga
     3241 cgagaagcag ggccagctct ggcgcgagga gatcgccgac ggcatcgccc ccggcaacac
     3301 gctgatgttc gcccacggct tcgcgatcct gtacggcgag atcgagcccg gcccgggcgt
     3361 cgacgtcggc ctggccgcgc cgaagggccc cggccacctg gtgcggcgcc agtacgagaa
     3421 gcaggagggc atccccggcc tggtggccat ccacgccgac gagtccggca acgcccgcga
     3481 cgtgatcctg gcctacgcga agggcatcgg ctgcacgcgc gcgggcgtga tcgagaccac
     3541 cttcaaggag gagaccgaga ccgacctgtt cggtgagcag gccgtgctct gcggcggcgt
     3601 gtccgagctg gtccaggcgg gcttcgagac gctcgtcgac gcgggctaca gcccgcagat
     3661 ggcgtacttc gagtgcctcc acgaggtcaa gctgatcgtc gacctgatgt acgagaaggg
     3721 gctggccggc atgcgctact cgatctccaa cacggccgag tacggcgact acacccgcgg
     3781 caagcgcgtg atcaccgacg agacccgcga gtcgatgaag aagatcctgg gcgagatcca
     3841 gtcgggcgag ttcgcccgcg agtggatcgc cgagaacaac gccggccagg agaacttcct
     3901 gcgcatgcgc caggagcagg ccgacaccca gatcgagcgc gagggcaagg agctgcgcag
     3961 caagatggac tggatcgact cggagttctg acgttggccg cagactcgcc agggcacggc
     4021 gggttcctgc cgcccgagcc gaccgggccc gagcccgacc tgggccggcg cccggagccg
     4081 gcggcggccc cgccgcccgc gccggcgtcg ggctacgagc cgcccgcgca cgcccagccc
     4141 gggtggcagc cgcccgcaca gccgcagggc tggcagcacc cccaggccgc cgcgccggac
     4201 aacggccccg ccgtcctggg cttcagcctc tcgatctcca gcctgggcct gctgatcatc
     4261 tccgccggcc tgtccaccct gctgtcggtg ggcctggcca tccccgggat ggtgcagtcg
     4321 cgcaagggca cccagcgggt gcgcgagggc gtgaccgacc gcaacgccgg cctggccaac
     4381 gcggggtgga tcctgggcat cgtcgcgctg gtgctgtccg ccctggccac catcttctgg
     4441 atcgtgatcg cgatcgtggt ggccaccgat gacgaggcgc gccgcgagtt cgagcgcgag
     4501 ttcgaccgcc agtacgacca gtcgtcgatg tccgcgctga tcgtggtcgc ccgcctggtt
     4561 ggagccgccc tgctgtgagg gccgctgcgg tggccctggt ggccgccatg gccctggtgc
     4621 ccgcgggcgc cgcgggaccc gccgaggcgg ccaagcgccc gacgcgcccg gtggtcatgg
     4681 tcgtgttcga cgagctgccg ctgacctcgc tgctggacgg acgcgggcgg atcgacaggg
     4741 tccgctaccc caacttcgcc gccctggcgg gcgacgccac ctggtacgcc aacgccacga
     4801 ccgtctccga cgccaccaag ctggccattc cctcgatcct ggacggcagg ctgcccaagg
     4861 tcggcgtccc ggccacccac cgcggccatc cgcggaacgt gttcacgctc ctgcgcggga
     4921 acggataccg gctgaaggtc caggaggagg ccaccagcct gtgtccctac cgcggctgcc
     4981 ggcgccgcca cggcgcccac tacttcctct cgcgcgaccg gctgggccgg ttcgagcgct
     5041 ggatcgcggg gatcggccgc tcgcgccgcc cgacgctcta ctacaagcac gcgctgcttc
     5101 cccacgtgcc gtgggtgttc acgccccagt tgcgccgcta cgaccgctcg gtgctgggcc
     5161 cgatcaaggg cctgaacagc agcgagcgga gcgtgttcga ccgcacgctc gtgcgccagt
     5221 cgtggcagcg ccacctgctc caggtgggcg cggtggacac gctgctgggc cggatcgtcg
     5281 cgaggatgaa ggccacgggc gtctacgacc gggcctcggt cgtgatcatg gccgaccacg
     5341 gggtgtcgtt ccacgtgggc gccaccgacc ggcgcacgat cctgcccttc aacgccttcg
     5401 acgtggcgcc gatcccgctg ttcatcaagg cgccgggcca gcgccggggg cggatcgacc
     5461 ggtcgatgat gcgcacctac gacgtgctgc ccaccgtcgc cgggcagatc gggcttcgcc
     5521 tcccgcgcgg cctctccggg cgctcggcca ccagcaaccg cgtgcgccgg cgcgggcgcg
     5581 tcaaggtcat gtcgcgcgcg ccgctggccc ggatcacctt cagccgcggg cgcctggcgc
     5641 agggccgccg cgccgcggtc cggcgcaagc tggcgctctt cggctcgggc acgcgcagcc
     5701 tgtacctctt cggcgccaac tcccagctgc tggggcgcca ggtcgcgagc ctgcgcgtgc
     5761 gccgccgcgg cgccgtccgg gccacgctca acggcgcccg cgactaccgg cgcatctacc
     5821 gccgctcccg cttcatcccc gcccacgtga cgggacggat caccggccgc cgccgcggcg
     5881 cgcggcgcga catcgcggtg gccgtgaacg ggcgcatctg ggccgtcacg cgcagcgtgc
     5941 gcctgcgcgg gagccgctcg gagtactact cggcgctgct gaaccccgac aagctcgtgg
     6001 tgggccgcaa ccgcaacgcc atcgccgtct acgaggtcac gcgctcgcgc ggcaagtacg
     6061 cgctgcgccg cctgcgctaa ggtctttcgc ccctcgcgac tggcgtctta ggtggatcgg
     6121 accaccgggg agcgagaggg aagcatctcc gccgcgcgcc tgggcagacc gttcgtacga
     6181 ccccgatcag gagccgtaag cccatgacga ccgccaccgt ggagattccc caagacctca
     6241 agcccgccga cggccgcttc ggctgcgggc cgtccaaggt ccgccccgag cagctggcca
     6301 gcctggccgc cgacggcgcc gcgatcatgg gcacctcgca ccgccagaag ccggtgaaga
     6361 gcctggtcgg ccgcgtgcgc cagggactgg cccagctcct gggcctgccc gagggctacg
     6421 aggtgctcct gggcaacggc ggcaccaccg cgctctggga cgcgctggcg ttcggcatcg
     6481 tccgcgagcg tgccctgcac ctgacctacg gcgagttctc gtccaagttc gccaaggtca
     6541 ccacgggcgc gccgttcctg gccgacccgg tggtcgtcga gtccgacccc ggcacggcgc
     6601 cggagctgag ctccgacgac tcctgcgatg tcgtcacgtg ggcgcacaac gagacgtcga
     6661 ccggcgtcat gctcggcgtc gagcgtcccg ccggggacgc gctcgtgctg gtggacgcca
     6721 cctccggcgc cggcgggctg ccgctgcacg cgcagaacgc cgacgtctac tacttcgccc
     6781 cgcagaagag cttcgccgcg gacggcggca tctggttcgc cgcctgcagc ccggcggcga
     6841 tcgagcggat cgaggagatc ggcgcgtccg accgctggat ccccgacttc ctgtcgctga
     6901 ccacggcgct ggagaactcg cgcaaggacc agacctacaa caccccggcg ctggccaccc
     6961 tgtggctgct ggccgagcag gtggactgga tgaacgccgg cggcggcctg caggagttct
     7021 gcgtggcccg cacccaggag tcctcgtcgc acctgtacag ctgggccgga agctcggagt
     7081 tcgcgtcgcc gttcgtggcc cagcaggcca accgctcgct ggtggtcggc acgatcgact
     7141 tcgacgactc cgtcgacgcc gccgccgtgg ccgccacgct gcgcgccaac ggcatcgtcg
     7201 acaccgagcc ctaccgcaag ctgggccgca accagctgcg catcggcatg ttccccgccg
     7261 tggagccgga cgacgtccgc gcgctcacgg cctgcatcga ctacgtggtg gaggcccagt
     7321 catgacgaag gttctcgtga aggagaagat cggcgacagc ggcgtgcagc tgctgcgcga
     7381 cgcgggcttc gacgtggagc tgggcgccga ctgggaggac ggcgagctcg agcggcgcat
     7441 cggcgagttc gacggcatcc tgatccgctc ggccaccaag ctgacggccg acctgatcga
     7501 gaaggccgac aacctcaagg ccgtcggccg cgccggcgtc ggcgtggaca acgtggacgt
     7561 ggacgccgcc accaagcgcg gcatcgtggt ggccaacgcg ccccagtcca acgtcgtcac
     7621 cgccgccgag cacacgatgg cgctgctgac cgcgatggcg cgcaacgtgc cccaggccca
     7681 ccagtcgctg gtcgagggcc gctgggaccg gtccaagtac agcggcgtgg agctgtacga
     7741 caagaccctg ggcatcatgg gcttcggccg catcggccag ctcgtggccg agcgcgcccg
     7801 cgccttcggc atgcgcgtgc tggccttcga cgcctacgtg gccgaggagc gcttccgcga
     7861 gctgggggcc gagcgcgccg acagctcgga ccaggtgtac gccgaggccg acttcatcac
     7921 cgtgcacctg cccaagacgc ccgagaccga gaactggctg gacgccgagg cgttcgcgaa
     7981 gatgaaggac ggcgtgcgca tcctgaacgt ggcccgcggg ccgctggtgg tggacgccga
     8041 cctggaggcc gccctggagt cgggcaaggt cgccggcgcc gcgctggacg tgttccgctc
     8101 ggagcccatc accgaccacc cgctgttcgg ccaccccaag gtcatcgtca cgccccacct
     8161 gggcgcgtcg acggccgagg ccaccgaccg cgccggcttc caggccgcgg agcaggtcgt
     8221 ggccgccctg accggcggcg tggtgaccac cgcggtcaac gtgcccgcgg tggcggggga
     8281 ggacctggag gcgctggggc cgtacctgcc gctggtctcc gacctgggcc ggatcggcac
     8341 cgcgctggcc gagggcacct ccatcgacgg catcgacgtg gagtacctgg gccggatcgc
     8401 cgagcgcgac acccggctgc tgaccgtcca ggtcatcaag ggcgccctga tgggccgcac
     8461 cgaggagcag gtcaacgacg tgaacgcgcc ctcgctggcg caggagcgcg gcatcgacgt
     8521 gcgcgagacc cgcagcacct cggcgcgcga cttcagcgac ctggtgcgtg tgaccgtgcg
     8581 cagcggggac gagcagaccc gcgtggtcgg caccgtgttc ggccgccgcg cgcggcccca
     8641 cctgctggag gcgtggggag cgcgcttcaa cgtccagctg gaggacagcc tggccatctt
     8701 ccgctaccgc gaccagcccg gccgcatcgg ccgcgtgggc acggtgctgg gcgacgccgg
     8761 cgtgaacgtg aactcggcgg cggtgggtca ccacggcgag gacgaggagc ccgaggaggc
     8821 ggtcatgatc gtgaccaccg acgccccggt gccccaggac gtggtcgacg agatcgtgtc
     8881 cgaggacggc ttcgtggccg ggcggacgat cacgctctag gcggttagcc tgcgcgcgtg
     8941 gcctggcagc ctccgccggt gcagtaccag cagcgcgcgc cccaggccaa caacgacctg
     9001 gcggtcgccg ccctgtgcat gtcgctcgcc agcctggggg tgctggtcgt gggcacgccg
     9061 ttcacgttcg gcttctcgct gctgctgtcg gggccgctgg ccatcggcgg cctggtctgc
     9121 ggggtgatcg ccaggcagaa ggccgaccgc ggcgaggccg gcgggcgcag catggcccag
     9181 gccgcgttcg tgctgggcat cgtgagcctg gtgctccacg tggtggcggt gatcctgggc
     9241 gtggcgctga tcgccgtcct gctcgagtcg atcgacgact tcgacgtgcc cgagcccgac
     9301 cagggcccgg aggcggatcc ggcctagggc cgtgcgtgta cgattgcccc gatgtcgttc
     9361 ccgctcgacc tccgccttct ccttctcctc ccccctcggg gggaatagcg cgtggcggcc
     9421 gcctaagccg caaagcccag gagatcgcag gaccgaggta gaagggaaca ccgaacgaca
     9481 tggcagagca gaccagcacc cgtagtgacg aagaccgcgt actgatcttc gacacgaccc
     9541 tgcgcgacgg ggagcagtcg ccggggatct cgctgaacaa gtccgagaag ctggagatcg
     9601 cccaccagct cgcccgcttg ggcgtggatg tgatcgaggc cggcttcccg atcgcctcgc
     9661 ccggggactt cgaggccgtc caggccatcg cccgtgaggt gcaggggccc atcatctccg
     9721 ggctggcgcg tgccggggcg ggtgacatcg acgccgcgtg gaacgcggtc agggagtcgg
     9781 accggccgcg gatccacacg ttcatctcca cgtcggacat ccacatcgag ca
//
LOCUS       DASUPW010000269         5952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_616154, whole genome shotgun sequence.
ACCESSION   DASUPW010000269 DASUPW010000000
VERSION     DASUPW010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616154"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VFY44_06150"
     CDS             <1..627
                     /locus_tag="VFY44_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910057.1"
                     /translation="ITPVLVTLFKEALDRPTAKIAEKVTVSSKALPQTRVREPAASHV
                     GRDAQLDEGPTRRLDPTEPGPRGPDGDGSEIRVYRQQPAKTGRMGRINPKVALITGLV
                     AFVIAGVVLTAGQLAIGNPFGDDGDGAIILGGGKNSKRNTETEPQNTTTQQQTVPEEQ
                     QTVPQQTTPEGQQTAPVAPPEQQQQQRAPTPTTPQNTTTTPAPGSVQP"
     gene            633..1103
                     /locus_tag="VFY44_06155"
     CDS             633..1103
                     /locus_tag="VFY44_06155"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HEX5910058.1"
                     /translation="MEAGSGRAETDEERLDRNLTELLAEMRVAITGVQVLFAFLLIVP
                     FNDGFKDLNTLQRDLYLVSLLGAGAATVALVAPIAQHRVTFRLQDKHHIVHSANRFAL
                     IGLACLALSMTAAVTLVTSAVFGTATGLVTAAGTLLAFAGCWGVLPVSRRRAIR"
     gene            1137..1325
                     /locus_tag="VFY44_06160"
     CDS             1137..1325
                     /locus_tag="VFY44_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910059.1"
                     /translation="MGIMDKITGRAKKAAGDVADDASLRQQGRKEEAKGEAKENAAAK
                     QESADAEKERAAKLERET"
     gene            complement(1329..2216)
                     /locus_tag="VFY44_06165"
     CDS             complement(1329..2216)
                     /locus_tag="VFY44_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910060.1"
                     /translation="MLTSAFLAAATVAGCPAFPADSHWNRRVDGLPLVSNSAAMIAAV
                     GADRRAHADFGSGLYEGRPIGIPYKVVKRGQKRVPVSFEYAGESDKGPYPIPKGAPVE
                     GGRSGDGDRHVIVVDRSRCKLYELFDAHPSAGDRSWRAGSGAVWNLRSNKLRPRGWTS
                     ADAAGLPIFPGLARYEEVKKGAIKHALRITVPRSRRAFIYPATHFASSLTDAALPAMG
                     QRVRLKRSFDASGYPRQSRVVLTALKRYGALVADNGSAWYVSGAPSKGWDNDDLSSLG
                     RVRGSDFEVVDSGRLGRPK"
     gene            complement(2206..2583)
                     /locus_tag="VFY44_06170"
     CDS             complement(2206..2583)
                     /locus_tag="VFY44_06170"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5910061.1"
                     /translation="MALHKGEGWSVGNIDDLGDGPGFRKIRRELDVSEMGVNAIVLPP
                     GVDPDFHLHERQEEVYFVHQGAVDIEFGDGTRHRLETGGLARVAAEVPRKLTTVGDQD
                     AVYLIFGAQGGYVGRDGLQLDAD"
     gene            complement(2593..2796)
                     /locus_tag="VFY44_06175"
     CDS             complement(2593..2796)
                     /locus_tag="VFY44_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910062.1"
                     /translation="MNEQQRDPEDLDAEVEDLEKAAEKVQDDIDETKTDWESKKGDES
                     VPGAVGEDDLGGETFPQEEDEGG"
     gene            complement(2834..3601)
                     /locus_tag="VFY44_06180"
     CDS             complement(2834..3601)
                     /locus_tag="VFY44_06180"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5910063.1"
                     /translation="MRFRSVALAVAWRNLHNFFKNPALVIPALLFPLFFFTAFAGGLS
                     SIGNAPGFEFPDGYTAFQFVFVILQASAFGGVFTGFAIARDFEVGFGRRLLLAAPNRT
                     AVLCGYAIAALARALFTWAMLTVVAFAVGMTISGNAGDLAALYLLGALLNLAAVLWAA
                     GIALRFRSLQAGPLMQTPVFMIIFLAPVYVPIDLLHGWVEVVAHGNPITPIVEAGRDF
                     ISGASAQTWLAYGCGLGLVALFGGWAVTGLRRAEAAG"
     gene            complement(3598..4377)
                     /locus_tag="VFY44_06185"
     CDS             complement(3598..4377)
                     /locus_tag="VFY44_06185"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5910064.1"
                     /translation="MSRATRIQIAALARRSFVRTLRQPAQVVPTVIFPLFLVAVNAGG
                     LEAATELPGFPTNSYLSFAIAVPFIQAAIFAVNNGGADLARDIETGFFDRLALTPMSG
                     GALIVAQLAGVVALGVLQSLIYLTIGVAFGASFLAGPAGVLVLIALALVIMFAFGCIG
                     LYFAFRTGSSEAVQGLFPVLFVFLFLSSGSLPRNLIEQDWFRTIATYNPVSYLIEGVR
                     SLFIVGWDPEALALAFGCAGGIAAVFMVLATTSLRTRLTRT"
     gene            complement(4374..5375)
                     /locus_tag="VFY44_06190"
     CDS             complement(4374..5375)
                     /locus_tag="VFY44_06190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5910065.1"
                     /translation="MRGNGDRAIDVEGLVKQFKKGPLAVDGIDLHVDPGEIYGFLGPN
                     GAGKSTTVLMLTTLLPPTGGTATVAGFDVATQGSEVRQHIGAALQEAALDPFLTAREH
                     MRLQTALHGLPRSERTRRGDELIERVGLSEAADRKVGGYSGGMRRRLDLALALVHGPR
                     ILFLDEPTTGLDPQSRTSLWEEVERLSSDDGVTVFLTTQYLEEADQLADRVGIIDDGH
                     IVAEGTPAALKAEIASPSVEAIPADPRRCEDVTRILAPFGDGEEPHRAGDGIAVRLAG
                     GESELAAVVRALDAENVEISHLQLHAPSLDDVFLAKTGRTLEGAGEDDDTAETPVAS"
     gene            5423..>5952
                     /locus_tag="VFY44_06195"
     CDS             5423..>5952
                     /locus_tag="VFY44_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910066.1"
                     /translation="MAARAPEEIIRDWLADNGPTRVGLDAFEQTWGIERMGPRDRRRV
                     AAALARVGVSCDPPLARVSRGERVLLAVEEVALEPEPVYEPEPEPVYEPEPVYEPPPE
                     PEPEPEPEPEPASSLFRRPWDRREPEPEPQPEPELELEPAPEDEWPEMEPWPEPASQE
                     PEPEPEPEPEAPKRIPA"
BASE COUNT          924 a         2164 c         2132 g          732 t
ORIGIN      
        1 atcacccctg tcctggtcac gctgttcaag gaggcgctgg accgccccac ggccaagatc
       61 gccgagaagg tcacggtgtc gtccaaggcg ctgccgcaga cccgggtccg cgagccggcc
      121 gccagccacg tgggccgcga cgcgcagctg gacgagggcc cgaccaggcg gctggacccg
      181 accgagcccg gcccccgtgg ccccgacggc gacggatccg agatccgcgt gtaccgccag
      241 cagcccgcca agacgggccg catgggccgc atcaacccga aggtggcgct gatcaccggg
      301 ctggtcgcgt tcgtgatcgc cggcgtggtc ctgaccgccg ggcagctggc catcgggaac
      361 ccgttcggcg acgacggcga cggcgcgatc atcctgggcg gcggcaagaa ctcgaagagg
      421 aacaccgaga ccgagccgca gaacaccacg acgcagcagc agacggtccc ggaggaacag
      481 cagaccgtgc cgcagcagac gacgcccgag gggcagcaga ccgcgccggt cgcgcccccg
      541 gagcagcaac agcagcagcg ggcgcccacg ccgacgacgc cgcagaacac caccaccaca
      601 ccggcgcccg gctccgtgca accctgaggg gcgtggaagc cggaagcggg cgcgccgaga
      661 ccgacgagga gcgactcgac cgcaacctga ccgagctgct ggccgagatg cgggtggcca
      721 tcaccggcgt gcaggtgctt ttcgcgttcc tgctgatcgt gccgttcaac gacggcttca
      781 aggacctgaa caccctgcag cgcgacctct acctggtcag cctgctgggc gccggggcgg
      841 ccacggtcgc cctggtggcg ccgatcgccc agcaccgggt gaccttccgg ctccaggaca
      901 agcaccacat cgtgcacagc gcgaaccggt tcgcgctgat cgggctggcc tgcctggccc
      961 tgtcgatgac ggccgccgtg accctggtca ccagcgcggt gttcggcacg gccaccgggc
     1021 tcgtcaccgc cgccggcacc cttctggcgt tcgccggctg ctggggcgtg ctcccggtga
     1081 gccgccgccg ggccatccgc tgattcgccg atctcgctcc gggtagcccg gcgggcatgg
     1141 gaatcatgga caagatcacc ggtcgtgcaa agaaggccgc aggagacgtc gccgacgacg
     1201 cctcgctgcg ccagcagggc cgtaaggaag aggccaaggg cgaggcgaag gagaacgccg
     1261 ccgccaagca ggagagcgcc gacgccgaaa aggagcgcgc ggccaagctc gagcgcgaga
     1321 cctgagcgct acttcgggcg gcccagccgc ccgctgtcga cgacctcgaa gtccgacccg
     1381 cggacgcggc ccaggctcga gagatcgtcg ttgtcccacc ccttcgacgg cgcgccggac
     1441 acgtaccacg ccgagccgtt gtcggccacc agcgcgccgt agcgcttgag cgcggtcagc
     1501 acgacgcgtg actggcgcgg atagcccgac gcgtcgaagg accgcttcag gcgcacgcgc
     1561 tggcccatcg cgggcagcgc ggcgtcggtg agcgacgacg cgaagtgcgt ggcggggtag
     1621 atgaacgccc gccggctgcg cggcaccgtg atgcgcagcg cgtgcttgat cgcgcccttc
     1681 ttgacctcct cgtagcgcgc caggcccggg aagatcggca ggccggccgc gtcggcggac
     1741 gtccagccgc gcgggcgcag cttgttcgag cgcaggttcc agaccgcgcc ggagccggcc
     1801 cgccacgagc ggtcgcccgc cgacgggtgc gcgtcgaata gctcgtacag cttgcagcgc
     1861 gaccggtcga ccacgatcac gtgacggtcg ccgtcgcccg agcggccgcc ctcgaccggc
     1921 gcgcccttcg ggatcgggta ggggcccttg tcggactcgc cggcgtactc gaagctcacg
     1981 gggacccgct tctgcccgcg cttgaccacc ttgtagggga tgccgatcgg ccggccctcg
     2041 tacagccccg agccgaagtc ggcgtgggcg cggcggtcgg ccccgacggc ggcgatcatg
     2101 gccgccgagt tggacaccag cggcaggccg tcgacgcggc ggttccagtg gctgtcggcg
     2161 gggaacgccg ggcacccggc caccgtggcg gcggccagga aggcgctagt cagcatcgag
     2221 ctggagcccg tcgcgtccca cgtagccgcc ctgcgcgccg aagatcaggt agacggcgtc
     2281 ctggtcgcca acggtcgtga gcttgcgcgg cacctcggcg gccacccgcg ccagcccgcc
     2341 cgtctccagc cggtgcctgg tcccgtcgcc gaactcgatg tcgaccgccc cctggtgcac
     2401 gaagtacacc tcctcctgac gctcgtggag gtggaagtcg gggtccacgc ccggcggcag
     2461 gacgatcgcg ttcaccccca tctccgagac gtcgagctca cgccggatct tgcggaatcc
     2521 gggtccgtct cccaggtcgt cgatgttgcc gacggaccag ccctctccct tgtgcagtgc
     2581 catggggcga gcctacccgc cctcgtcctc ctcctggggg aaggtctcgc cgcccaggtc
     2641 gtcctcgccg acggcgcccg ggaccgactc gtcgcccttc ttggactccc agtcggtctt
     2701 ggtctcgtcg atgtcgtcct ggaccttctc ggccgccttc tcgaggtctt cgacctcggc
     2761 gtccaggtcc tccggatccc gctgctgttc gttcatggac gccgcctacc ccggatccgc
     2821 ccgtggcaac cggtcagccg gcggcttcgg cgcggcgcag gcccgtcacg gcccagccgc
     2881 cgaagagcgc caccaggccc aggccgcaac cgtaggccag ccacgtctgg gccgaagcgc
     2941 cggagatgaa gtcgcggccc gcctccacga tcggcgtgat cgggttcccg tgggccacga
     3001 cctcgaccca gccgtgcagc aggtcgatcg gcacgtagac cggcgccagg aagatgatca
     3061 tgaacacggg cgtctgcatc agcgggccgg cctgcagcga gcggaaccgc agcgcgatcc
     3121 cggccgccca cagcaccgcg gccaggttga gcagcgcgcc cagcaggtag agggcggcca
     3181 ggtccccggc gttgccgctg atggtcatgc ccacggcgaa cgcgaccacg gtgagcatgg
     3241 cccaggtgaa cagcgcgcgc gccagcgcgg cgatggcgta cccgcagagg acggcggtgc
     3301 ggttcggcgc cgccaggagc aggcggcggc cgaagccgac ctcgaagtcg cgggcgatgg
     3361 cgaagccggt gaacacgcca ccgaaggccg acgcctggag gatcacgaac acgaactgga
     3421 acgccgtgta gccgtcgggg aactcgaagc cgggcgcgtt gccgatgctg gacaggccgc
     3481 cggcgaaggc ggtgaagaag aacagcggga acagcagcgc cgggatcacc agcgccgggt
     3541 tcttgaagaa gttgtgcagg ttgcgccagg ccaccgccag cgccacggac cggaacctca
     3601 tgtgcgcgtc agcctggtgc gcagggacgt ggtggccagg accatgaaga ccgcggcgat
     3661 cccgccggcg cagccgaacg ccagcgccag cgcctcgggg tcccagccga cgatgaacag
     3721 gctgcgcacc ccctcgatca ggtagctgac cgggttgtag gtggcgatcg tgcggaacca
     3781 gtcctgctcg atcaggttgc gcggcagcga gccggaggac aggaacagga acacgaacag
     3841 caccgggaac aacccctgga ccgcctcgct gctgccggtg cggaaggcga agtacaggcc
     3901 gatgcagccg aacgcgaaca tgatcaccag cgccagcgcg atcagcacca gcacacccgc
     3961 cgggcccgcg aggaacgacg cgccgaaggc gacgccgatg gtcaggtaga tcagcgactg
     4021 gagcacgccg agcgccacca cgcccgccag ctgggccacg atcagcgcac cgccgctcat
     4081 cggcgtcagc gccaggcggt cgaagaagcc cgtctcgatg tcgcgcgcca ggtcggcgcc
     4141 gccgttgttc acggcgaaga tggccgcctg gatgaacggc acggcgatgg cgaagctcag
     4201 gtacgagttc gtcgggaagc ccgggagctc ggtcgccgcc tccagcccgc cggcgttcac
     4261 cgcgacgagg aacagcggga agatcaccgt gggcaccacc tgcgcgggct ggcgcagggt
     4321 gcgcacgaac gagcgccgcg ccagcgcggc gatttgtatc cgcgtcgccc tgctcacgag
     4381 gcgaccggcg tctcagccgt gtcgtcgtcc tcgccggccc cctccagggt gcggccggtc
     4441 ttggccagga acacgtcgtc cagcgacggc gcgtgcagct gcaggtgcga gatctccacg
     4501 ttctcggcgt ccagcgcgcg gaccaccgcg gccagctcgg actcgccgcc ggccaggcgg
     4561 acggcgatgc cgtcgcccgc gcggtggggc tcctcgccgt cgccgaacgg ggccaggatg
     4621 cgcgtcacgt cctcacaccg gcgcgggtcg gcggggatgg cctccaccga gggcgaggcg
     4681 atctcggcct tcagcgcggc cggggtgccc tcggcgacga tgtggccgtc gtcgatgatc
     4741 cccacgcggt cggccagctg gtcggcctcc tccaggtact gcgtggtcag gaagaccgtc
     4801 acgccgtcgt cggaggacag ccgctcgacc tcctcccaca gcgatgtgcg gctctgggga
     4861 tccaggcccg tggtgggctc gtccagaaac aggatccgcg ggccgtgcac cagcgccagc
     4921 gccaggtcca ggcggcggcg catgccgccc gagtagccgc cgaccttgcg gtcggccgcc
     4981 tcggacaggc cgacgcgttc gatcagctcg tcgccgcggc gcgtgcgctc ggagcgcggc
     5041 agcccgtgca gggcggtctg caggcgcatg tgctcgcgcg ccgtcaggaa cgggtccagc
     5101 gcggcctcct gcagcgcggc gccgatgtgc tggcggacct ccgacccctg tgtggccacg
     5161 tcgaagccgg cgacggtcgc ggttcctccg gtgggcggca gcagggtggt cagcatcagc
     5221 accgtggtgg acttgcccgc tccgttgggg cccaggaagc cgtagatctc gcccgggtcc
     5281 acgtgcaggt cgatcccgtc cacggccagc ggccccttct tgaactgctt gaccagaccc
     5341 tcgacgtcga tcgccctgtc gccgttcccg cgcacccgta gaacgtagcg gtgaggcgtt
     5401 cgggcacgta cgcttgagga cgatggctgc ccgcgccccg gaggagatca tccgcgactg
     5461 gctcgccgac aacggcccga cgcgggtggg cctggacgcg ttcgagcaga cctggggcat
     5521 cgagcgcatg gggccgcgcg accgccgccg ggttgccgcc gccctggcac gggtcggcgt
     5581 gtcctgcgat ccgccgctgg cccgggtctc ccggggcgag cgggtgctgc tggcggtcga
     5641 ggaggtcgcg ctggagccgg agccggtcta cgagcccgag ccggagccgg tctacgagcc
     5701 cgagccggtg tacgagccgc ccccggagcc ggagcctgaa cccgagccgg agcccgagcc
     5761 ggcctccagc ctcttccggc gaccgtggga ccggcgcgag ccggagcccg agccgcaacc
     5821 ggagccggag ctcgagctcg agccggcgcc cgaggacgag tggcccgaga tggagccgtg
     5881 gcccgaaccg gcgagccaag agccggaacc ggagccggaa cccgagcccg aggctccgaa
     5941 gcgcatcccg gc
//
LOCUS       DASUPW010000270         4090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1308876, whole genome shotgun sequence.
ACCESSION   DASUPW010000270 DASUPW010000000
VERSION     DASUPW010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308876"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VFY44_06200"
     CDS             <1..192
                     /locus_tag="VFY44_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910067.1"
                     /translation="CTANEWSVNNYSAGVSPAPTVIYADDARIDANAPGPVGPAPPSP
                     GPAPAPSPAPGGPLCPPIC"
     gene            complement(210..282)
                     /locus_tag="VFY44_06205"
     tRNA            complement(210..282)
                     /locus_tag="VFY44_06205"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(247..249),aa:Arg,seq:ccg)
     gene            383..1930
                     /locus_tag="VFY44_06210"
     CDS             383..1930
                     /locus_tag="VFY44_06210"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX5910068.1"
                     /translation="MRIRAVTLPALAVALCLGLTPGTAQASLSGTPVEVASGLETPWD
                     VLRLPDGRTLVTEIGGRVRVIGADGALRAAPVYADSTAKKFLGMAADPGYATNRHVYL
                     YVGYGSGANPNASRIIRLTDDGTNLVSPVTIFNGGIASDDNHDGGRIAFGPDCMLYAT
                     TGDIHQPSLPQNLDSLNGKILRMTATGAAPADNPFPGAGARGFVWSYGHRHPQGIAWD
                     AEGRMWESEHGPSGEGHAPDGAKTGNDEINRIDKGANYGWPVIAGGQTAPGMRTPVTH
                     ASNSPAWAPGGLAIGPDRVMYAPFLAGTELRAFTIAGDSITSQTTLYDNTFGRLRAAT
                     ADATHLWLTTHNRTNNEKVLRVPFEPAGISAPAAKCTAAAPAAPTPARPAARLTRAQL
                     RAIVRGVLGRQAKALRSKRARKLAKVSKLRLTDRALPAGKLSIMVERPAIRRKRARVK
                     VLDGKATTRAGRRAAVTTKLGRQSRKLLRAAARRRGRLKLTLHVGLRTADKRLTTGKR
                     TITILRR"
     gene            1956..2444
                     /locus_tag="VFY44_06215"
     CDS             1956..2444
                     /locus_tag="VFY44_06215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX5910069.1"
                     /translation="MLLAVISDTHLPRGQRRLPDDCVGRLEQADLILHGGDVVSATTL
                     AEIELIGPPVVAVQGNQDDDELRRLLPVQRTVEAEDARIAMVHDAGPKDGRLRRLRLL
                     FPDADAVVFGHSHIPLHETDDGFQIFNPGSPTDRRRQPAFTMGLAHVSGDRVRFELVE
                     VG"
     gene            2447..3373
                     /locus_tag="VFY44_06220"
     CDS             2447..3373
                     /locus_tag="VFY44_06220"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEX5910070.1"
                     /translation="MDLDVLFLGTAASAPTARRGLPATLIRRGGEKLLVDCGEGTQRQ
                     LVRSVGLVDIDDLFITHFHADHVLGIPGLLKTYGLQARERRLRVYGPTGLERLFKSLA
                     PLIGRLPFEIQLTELDAGDELPGEGYRLTCFNVDHGVPALGYALVEDERPGRFDQAKA
                     QELGVQPGPDYGRLQAGETVDGVTPDQVLGPARAGRTVVLAGDSAPCDMTAAEAQGAD
                     LLVHEATFMAEDSPRARETRHSTAGGAARLAAEAGVKLVALNHISSRYYVRDLVAEAK
                     AEFGSVIVPRDFDRVELPFPESGEPRHVRGDE"
     gene            3459..4025
                     /gene="pyrR"
                     /locus_tag="VFY44_06225"
     CDS             3459..4025
                     /gene="pyrR"
                     /locus_tag="VFY44_06225"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX5910071.1"
                     /translation="MSHKVVLDRDDVRRTLVRIAHEIVEKNPGEAIAIVGIHRRGALL
                     AGRLRELVAELVGEPLPLGDVDISFYRDDVGLREPGAQPVVHASHIDFSVESHTVVLV
                     DDVLFTGRTVRAAIDALFDYGRPGRVQLAVLADRGHRELPIRPDYVGKNLPTARDERV
                     YVNLEELDGEDQVLITPEDAEIKIRRGP"
BASE COUNT          596 a         1544 c         1438 g          512 t
ORIGIN      
        1 tgcaccgcca acgaatggtc ggtcaacaac tactcggccg gcgtgtcgcc cgctcccacg
       61 gtcatctacg ccgacgacgc gcgcatcgac gcaaacgccc cgggcccggt gggcccggcg
      121 ccgccctctc cgggtcccgc gcctgcgccg tcgcccgcgc cgggcgggcc gctgtgccca
      181 ccgatctgct gagacgcggc tggagccgac gcgcccggga ggattcgaac ctccgacctc
      241 tcgctccgga ggcgagcgct ctatccactg agctacgggc gccggtcgcg ccagtctagt
      301 gtcgcgggga ccgggcggac atccgtccaa cgcgaggtca aggtggcgca gccggtccgg
      361 gaccggggtc catacttgcc gcatgcgaat ccgcgccgtc acgctgccgg ccctggccgt
      421 cgcactctgc ctcggcctga cgcccggcac cgcccaggcg tcgctgagcg gcacccccgt
      481 cgaggtggcg tccgggctgg agacgccctg ggacgtcctg cgcctgcccg acgggcgcac
      541 gctggtgacc gagatcggcg gtcgcgtgcg cgtgatcggc gccgacggtg cgctccgcgc
      601 cgcgcccgtg tacgcggaca gcacggccaa gaagttcctg ggcatggcgg ccgaccccgg
      661 gtacgcgacc aaccgccacg tgtacctgta cgtcggctac ggctccggcg ccaaccccaa
      721 cgccagccgc atcatccggc tgaccgacga cgggaccaac ctggtctcgc cggtgacgat
      781 cttcaacggc gggatcgcca gcgacgacaa ccacgacggc gggcggatcg cgttcggccc
      841 cgactgcatg ctctacgcga ccaccgggga catccaccag ccgtcgctgc cccagaacct
      901 ggactcgctg aacggcaaga tcctgcgcat gacggcgacc ggcgccgcgc cggccgacaa
      961 cccgttcccg ggggccggcg cccgcggctt cgtctggagc tacggccacc gtcacccgca
     1021 gggcatcgcc tgggacgcgg agggccgcat gtgggagtcc gagcacggcc cctccgggga
     1081 gggacacgcg ccggacggcg ccaagacggg caacgacgag atcaaccgga tcgacaaggg
     1141 ggcgaactac ggctggcccg tcatcgcggg cgggcagacc gcgcccggca tgcgcacccc
     1201 ggtcacccac gcgtccaact cgccggcctg ggcgccgggc ggcctggcca tcgggccgga
     1261 ccgggtcatg tacgcgccct tcctcgctgg cacggagcta cgcgcgttca cgatcgccgg
     1321 cgactcgatc accagccaga cgacgctgta tgacaacacc ttcgggcgcc tgcgggccgc
     1381 gaccgccgac gccacccacc tgtggctgac cacccacaac cgcaccaaca acgagaaggt
     1441 gctgcgggtg cccttcgagc cggccggcat cagcgcgccc gccgcgaagt gcacggctgc
     1501 cgcgcccgcg gcgcccacac cggcccggcc cgccgcgcgc ctcacgcgcg cccagctgcg
     1561 ggcgatcgtg cgcggggtgc tcggccgtca ggccaaggca ctgcgctcga agcgggcccg
     1621 aaagctcgcg aaggtgtcga agctgcgcct gaccgaccgg gcgctgccgg ccgggaagct
     1681 gtcgatcatg gtcgagcgcc cggcgatccg gcgcaagcgg gcccgcgtca aggtccttga
     1741 cggcaaggcc accacgcggg ccgggaggcg cgccgccgtg accaccaagc tcggccgcca
     1801 gagccgcaag ctgctgcgtg ccgccgcgcg gcgcaggggc cggctgaagc tgaccctgca
     1861 tgtcggcctg cgcacggcgg acaagcgcct gaccacgggc aagcgcacga tcaccatcct
     1921 gcgccgctga ccgcgggcgc atatcgtcgg cagccatgct gctcgccgtg atctccgaca
     1981 cccacctgcc gcgcggacag cggcgcctgc ccgacgactg cgtcggccgg ctcgagcagg
     2041 ccgacctgat cctgcacggc ggtgacgtgg tgtcggccac gaccctggcc gagatcgagc
     2101 tgatcgggcc gccggtcgtg gccgtgcagg gcaaccagga cgatgacgag ctgcggcgcc
     2161 tgctgcccgt ccagcgcacg gtcgaggccg aggacgcgcg gatcgcgatg gtccacgacg
     2221 ccgggccgaa ggacgggcgc ctgcgccgcc tccggctcct gttccccgac gccgacgcgg
     2281 tcgtgttcgg ccactcgcac atcccgctgc acgagaccga cgacggcttc cagatcttca
     2341 accccggcag cccgaccgac cggcgccggc agccggcctt cacgatgggc ctggcccacg
     2401 tgtcgggcga ccgcgtccgg ttcgagctgg tcgaggtcgg ctgaccgtgg acctggacgt
     2461 cctgttcctg gggaccgcgg cgtcggcccc gaccgcccgc cgcggcctgc ccgccacgct
     2521 gatccgccgc ggcggggaga agctgctggt cgactgtggc gagggcacgc agcgccagct
     2581 ggtgcgctcg gtggggctgg tggacatcga cgacctgttc atcacccact tccacgccga
     2641 ccacgtgctg gggatccccg ggctgctcaa gacctacggg ctccaggccc gcgagcggcg
     2701 cctgcgggtc tacgggccga ccggcctgga acgcctgttc aagtccctcg ccccgctgat
     2761 cgggcggctg ccgttcgaga tccagctgac cgagctggac gcgggcgacg agctgccggg
     2821 ggagggctac cgcctgacct gcttcaacgt ggaccacggc gtgccggcgc tgggctacgc
     2881 gctggtcgag gacgagcgcc ccggccggtt cgaccaggcg aaggcccagg agctgggcgt
     2941 ccagcccggc cccgactacg gccgcctcca ggccggggag acggtggacg gggtcacgcc
     3001 ggaccaggtc ctcggccccg cgcgcgctgg ccgcaccgtg gtgctggccg gcgacagcgc
     3061 cccgtgcgac atgaccgcgg ccgaggccca gggcgccgac ctgctggtgc acgaggccac
     3121 gttcatggcc gaggactccc cccgtgcgcg cgagacacgc cattcgaccg ccggaggcgc
     3181 tgcccggctg gccgccgagg cgggggtgaa gctggtggcg ctgaaccaca tctcctcgcg
     3241 ctactacgtg cgcgacctgg tggccgaggc gaaggcggag ttcggctcgg tgatcgtccc
     3301 gcgtgacttc gaccgagtcg agctgccgtt cccggaaagt ggggaaccga ggcacgtgcg
     3361 cggcgacgag taggtatgcc gccgccgggg ggagtgctag cttcacctga gcagttcctt
     3421 taacccggtc ccgcgaggcc aggaaggagc aagacagctt gtcccacaag gtggtcctcg
     3481 accgcgacga cgtgcgccgc acgctggtgc gcatcgctca cgagatcgtc gagaagaacc
     3541 ccggcgaggc catcgccatc gtgggcatcc atcgtcgcgg cgcgctgctg gccgggcgac
     3601 tgcgcgagct cgtcgccgag ctggtcgggg agccgctccc gctgggtgac gtggacatct
     3661 ccttctaccg cgacgacgtc ggcctgcgcg agcccggcgc ccagcccgtc gtgcacgcct
     3721 cgcacatcga cttctccgtc gagagccaca cggtggtgct ggtggacgac gtcctgttca
     3781 ccggccgcac cgtccgtgcc gcgatcgacg ccctgttcga ctacggccgc cccggccggg
     3841 tgcagctggc cgtgctggcc gaccgaggcc atcgcgagct gccgatccgg cccgactacg
     3901 tgggcaagaa cctgcccacc gcccgcgacg agcgggtcta cgtgaacctc gaggagctgg
     3961 acggcgagga ccaggtgctg atcactcccg aggatgccga gatcaagatc cggaggggcc
     4021 cgtgaagaaa catctcctgt ccatcgacga cctgacgcgc caggacatcg agcgcatcct
     4081 ccaccgggcc
//
LOCUS       DASUPW010000271         2325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1864444, whole genome shotgun sequence.
ACCESSION   DASUPW010000271 DASUPW010000000
VERSION     DASUPW010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1864444"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VFY44_06230"
     CDS             complement(<1..548)
                     /locus_tag="VFY44_06230"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX5910072.1"
                     /translation="MPPSRTPRAKRPVEYSILTTATLCTLAVGAVMVYSASSAESLLQ
                     GNGGDPMMYLKRYVFLGLIGLVAMYWFSRNGLAAVKRLTPAILGVAFALTVAVMLPGI
                     GVTINGATRWLGAGPLQFQPSELLKVALVLYTARLLAERPASVRQLGTLFKPLLLVVG
                     AACLLLLKQPDMGTAMVICFSIG"
     gene            complement(579..1781)
                     /gene="murD"
                     /locus_tag="VFY44_06235"
     CDS             complement(579..1781)
                     /gene="murD"
                     /locus_tag="VFY44_06235"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX5910073.1"
                     /translation="MTRPALPPAPYLVVGLKRSGLAAARMLAPHGEVIGVDSGQAEVP
                     GDFEAHVGSDGVDLLDRVATVVKSPGVPRQAPVVAAALERGIEVTGELELAWRLLDGH
                     PFVAVTGTNGKTTTVELLGAIWRQAGLPVEVAGNVGRPLGDLVGTAEPGATVICEASS
                     FQLEDASAFAPDCAVLLNVEPDHLDRHGTLEAYRAAKMRIFANDPPHTVMPADLDRDP
                     FDLGSPRLRGDHNRQNARAAALAADAMGVPRDAIVAALEAFAGVPNRLEEVATVGGVL
                     FVNDSKATNPASAVKGLESFPGGVHAILGGSLKGGGFEALRGPVAERCAAAYLIGEAA
                     PTLASDLQGTVELHECATLERAVARAAEAAEPGEVVLLSPACASFDAFADYEARGRRF
                     RELVAELG"
     gene            1812..2138
                     /locus_tag="VFY44_06240"
     CDS             1812..2138
                     /locus_tag="VFY44_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910074.1"
                     /translation="MALQMAATIFMGDTGSLGLGGAIAGLAVMTKTEVLLLLIGGIFV
                     IEALSVLIQVFAFQRFRRRVFLMAPIHHHFEMMAWSETKIILRFWIVAAICSAIGFTL
                     YQQSIA"
     gene            2135..>2325
                     /locus_tag="VFY44_06245"
     CDS             2135..>2325
                     /locus_tag="VFY44_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910075.1"
                     /translation="MTRPALPPAPYLVVGLKRSGLAAARMLAPHGEVIGVDSGQAEVP
                     GDFEAHVGSDGVDLLDRVAT"
BASE COUNT          322 a          850 c          839 g          314 t
ORIGIN      
        1 ccgatcgaga agcagatgac catcgcggtg cccatgtcgg gctgcttgag cagcagcagg
       61 cacgcggcgc ccacgaccag cagcagcggc ttgaacaggg tgcccagctg cctgaccgag
      121 gccggccgct cggccagcag ccgcgcggtg tagagcacca gcgccacctt cagcagctcc
      181 gagggctgga actgcagcgg cccggcgccc agccagcggg tggcgccgtt gatggtcacg
      241 ccgatgccgg ggagcatcac cgcgacggtc agcgcgaacg ccacgcccag gatggccggg
      301 gtcaggcgct tgaccgcggc caggccgttg cgcgagaacc agtacatggc caccaggccg
      361 atcaggccca ggaagacgta ccgcttgagg tacatcatcg ggtcgccgcc gttgccctgg
      421 agcagcgact cggccgagct ggccgagtag accatgaccg cgcccacggc gagggtgcag
      481 agggtggcgg tcgtgaggat cgagtactcg actgggcgct ttgcgcgcgg ggtacggctc
      541 ggcggcatca gggcctgttt cgacgcccat ccgcggattc atcccagctc tgcgacgagt
      601 tcccggaagc gccggccgcg ggcctcgtag tcggcgaacg cgtcgaagct ggcgcaggcc
      661 ggagagagca gcacgacctc ccccggctcg gccgcctcgg cggcgcgggc gaccgcccgc
      721 tccagcgtcg cgcactcatg cagctccact gtgccctgaa ggtcggacgc cagcgtcggg
      781 gcggcctccc cgatcaggta cgccgcggcg cagcgctcgg ccacggggcc gcgcagggcc
      841 tcgaagccgc cgcccttgag gctgccgccc aggatggcgt gcacgccccc cgggaacgac
      901 tccaggccct tgaccgccga ggcggggttg gtggccttgg agtcgttcac gaacagcacg
      961 ccgcccaccg tggccacctc ctccagccgg ttgggcacgc cggcgaacgc ctccagcgcc
     1021 gcgacgatgg cgtcgcgggg cacgcccatg gcgtcggcgg ccagggccgc ggcccgcgcg
     1081 ttctggcggt tgtggtcgcc gcgcaggcgc ggcgagccga ggtcgaacgg gtcccggtcc
     1141 aggtcggcgg gcatgaccgt gtgcggcggg tcgttggcga agatccgcat ctttgccgcc
     1201 cggtaggcct ccagcgtgcc gtggcggtcc aggtggtcgg gctccacgtt caacagcacc
     1261 gcgcagtcgg gcgcgaacgc cgaggcgtcc tccagttgga agctcgaggc ctcgcagatg
     1321 accgtggccc cgggctcggc ggtgcccacc aggtccccga gcgggcggcc gacgttgccg
     1381 gccacctcca ccggcagccc ggcctggcgc cagatcgcgc ccagcagctc caccgtcgtc
     1441 gtcttgccgt tggtgccggt cacggccacg aacggatggc cgtccaggag ccgccacgcc
     1501 agctccagct cgcccgtgac ctcgatgccg cgctccagcg ccgccgcgac caccggggcc
     1561 tggcgcggca cgcccggcga cttgaccacc gtggccacgc ggtccaggag gtcgaccccg
     1621 tcggagccca cgtgggcctc gaagtcgccg ggcacctcgg cctgacccga gtccacgccg
     1681 atcacctccc cgtggggggc gagcatgcgc gcggcggcga ggccggaccg cttgaggccg
     1741 acgaccaggt agggggccgg cgggagcgcc gggcgggtca tgcgatcgac tgctggtaga
     1801 gcgtgaagcc gatggcgctg cagatggcgg cgacgatctt catgggagac acggggtcgc
     1861 tcgggttagg cggcgccatc gcggggcttg ccgtgatgac gaagaccgag gtcctgctgc
     1921 tgctgatcgg cggcatcttc gtgatcgagg cgctgtccgt cctgatccag gtcttcgcct
     1981 tccagcgctt ccgccggcgg gtgttcctga tggccccgat ccaccaccac ttcgagatga
     2041 tggcctggtc ggagaccaag atcatcctgc gcttctggat cgtcgcggcc atctgcagcg
     2101 ccatcggctt cacgctctac cagcagtcga tcgcgtgacc cgcccggcgc tcccgccggc
     2161 cccctacctg gtcgtcggcc tcaagcggtc cggcctcgcc gccgcacgca tgctcgcccc
     2221 ccacggggag gtgatcggcg tggactccgg ccaggccgag gtgcccggcg acttcgaggc
     2281 ccacgtgggc tccgacgggg tcgacctcct ggaccgcgtg gccac
//
LOCUS       DASUPW010000272         5540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_480048, whole genome shotgun sequence.
ACCESSION   DASUPW010000272 DASUPW010000000
VERSION     DASUPW010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480048"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..975)
                     /locus_tag="VFY44_06250"
     CDS             complement(160..975)
                     /locus_tag="VFY44_06250"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX5910076.1"
                     /translation="MSTLTLIAALEDDLGDALARIASALRIPVLLAALLVLLASALEL
                     GRFVMELVRRGLPGGRTPLAALAHRAEAEPENARELARKAPSRPSEQAVLQLAAAASN
                     GNDKVAAEHALADYELAVQRRLDRTRIIVRAGPAVGLMGTLIPLAPGLAALGRGDLPQ
                     LASDLRTAFAATVIGLLAGTVAFALTLTRTRIYSEDLTALERAAAAIGTKPPPPVPTP
                     REKKRERAEQERAEKEKQKAEKAEKAKAEQEAAGKEPQPGEAKPAEAKPEGAA"
     gene            complement(972..2399)
                     /locus_tag="VFY44_06255"
     CDS             complement(972..2399)
                     /locus_tag="VFY44_06255"
                     /inference="COORDINATES: protein motif:HMM:NF024640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEX5910077.1"
                     /translation="MASLRRLSAVALAAFLLLPAAAAGAQERQRLESTVRYLQEVQHK
                     DGGFGERGSDPGFSTWAALALASNGINPRDQRKPGGVDVYTYLTRHTRGLTQSTDFAR
                     VILVATAAGTSPQRFGPIDPLGRLLRLQRRDGGFPQPPDIPGSTINGSAYAVLALAGI
                     DPSRVRGVSATQLRRSQARALDWLQERQSPDGSWGGTDLTGSVLEALNAGGRRNTAAQ
                     AGALEYLRSRQNADGGFGDGNAGAGSNSASTSWVLRGLAAARVSARSFKGAGSGRTPL
                     DYLASMQQPDGSVRYSAASDVDRVWNTSYAAPALAGTSLPIPAVDRDDPEDKSSQRDE
                     TPTRTRGDDGTQPGAGGLADGGDGDVTAGGGGEGAPLYSQPEPDSLGAQPGGERDTDT
                     PSRATGQGEGQVAGESVAGKLVGQGTLNAAGKGRSLGSAPGLRAAAAGGQAPTWLVAA
                     IAVALALSALGGIYLERRGVRKEDT"
     gene            complement(2377..3753)
                     /locus_tag="VFY44_06260"
     CDS             complement(2377..3753)
                     /locus_tag="VFY44_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910078.1"
                     /translation="MALTASSPAAATVVNVRIEGRSTTLFEGPVQTTGRNVQAVSERA
                     TGTIHHCDGTNNGANPGRVATPTASAVDAMSIVGQGFEGRWNANIEDYFVRGFGPDLE
                     TGPAYWGVLVGDNLTSVGGCQTALGENAEVLWAYDAFSGRPFLGLAGPGTATPGVAQG
                     FQVTQSGGSPKPIAEAGLTSSPAATITGGSGSFSVTFPGDGWYRLKADGARFIRSNRI
                     YVCVGACGPAPADAQVRVPPAPVFYGPGGAVLRDSPALAGTTAKVRITRPAAGTSGYR
                     RGRIVVRWRVAEQGVGLLRWAVASDDLTSRSKRYVTRARGTSATSASLRLPAGRVHAL
                     RFTSTDRMLRQDTIDFGRVIVPVDERAKSVRRTGPWRKLRSGKAWKGTLLRGRPGARI
                     RVKLAAGRPALLVRGRRAAVVRIGAKSVRVRPGRRTVLGSKRRRAGTVTVSVTRGSID
                     LDGIAASP"
     regulatory      complement(3814..4003)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     regulatory      4072..4194
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4273..>5540
                     /locus_tag="VFY44_06265"
     CDS             4273..>5540
                     /locus_tag="VFY44_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910079.1"
                     /translation="MARTPSARLTTAACLLALAITPASAHATSSGADIRASVSDGADW
                     AQTRQSTTTGAFPVFGFDIVPTALAAAGRNAADVSRGGGPTSQAYVAGALQSLAYDPS
                     AASTVGDIEQGILSGYAAGIDPARVSAGKNVVAELAGAYDDGRYGPRFSFNNTVYGAL
                     ALNRVRAPRFLVDRTVAAIRANQHNDGGWSFTESVTPADQANPSATDTTGPALAALCE
                     SGVPANDPAVEQAVSFLRSKLDLGTGGFDPQDGFGTPPNADTNGAVISGLNACGIDPQ
                     GPQFTGPGGQTPVDFLIGLQLTAGADAGGFPSAFSGASGDVYATQDAVRALAGAAFTA
                     APPARTTPTDPRTRPVPAVADGTSVPVALAVDDGSGGVTFCRVTVPSGASLATLLATA
                     NGSSSPAGCATGGQFTAGQVSRLNGQTGTWA"
BASE COUNT          666 a         2102 c         2051 g          721 t
ORIGIN      
        1 tgacgatgcg gccggtgtcg ctgttgatgg tcaggctgag gccggcgacc aggaaggcga
       61 cggccagcac gattcccaga tcgaacaggt tgaccagccc gtccagggga tcgcccgcgc
      121 tgtcctccag gcggccgtgg cggcccaggc cgacgcggct cacgccgcgc cctcgggctt
      181 ggcctccgcc ggcttggcct cgcccggctg cggctccttg ccggcggcct cctgctcggc
      241 cttggccttc tcggccttct cggccttctg cttctccttc tcggcgcgct cctgctccgc
      301 ccgctcgcgc ttcttctcgc gcggcgtggg caccggcggg ggcggcttgg tgccgatggc
      361 ggccgcggcg cgctcgaggg cggtcaggtc ctcgctgtag atgcgcgtac gggtcagcgt
      421 cagggcgaac gcgacggtgc cggccaggag gccgatgacg gtggcggcga acgccgtgcg
      481 caggtcggac gccagctgcg gcaggtcgcc gcggccgagg gcggccaggc ccggcgccag
      541 cgggatcagc gtgcccatga ggccgacggc cgggccggcg cggacgatga tccgcgtgcg
      601 gtccaggcgc cgctgcaccg ccagctcgta gtcggccagc gcgtgctcgg ccgcgacctt
      661 gtcgttgccg ttcgaggcgg cggcggccag ctggagcacc gcctgctcgc tgggccggct
      721 cggggccttg cgggccagct cgcgcgcgtt ctcgggctcg gcctcggcgc ggtgggccag
      781 cgcggcgagc ggtgtgcgcc ccccgggcag cccgcggcgc accagctcca tgacgaaccg
      841 gcccagctcc agggccgagg ccagcagcac gagcagcgcg gccagcagca ccgggatccg
      901 cagcgcggac gcgattcggg ccagcgcgtc gcccaggtcg tcctccaggg cggcgatcag
      961 ggtcagcgtg ctcaagtgtc ctccttgcgg actccgcgac gttcgaggta gatgccgccc
     1021 agggcgctga gcgccagggc gacggcgatg gcggccacca gccaggtggg cgcctggccc
     1081 ccggcggcgg ccgcgcgcag gcccggggcc gagcccaggc tacggccctt cccggcggcg
     1141 ttcagcgtgc cctggcccac gagcttgccg gccacggact cgcccgcgac ctgtccctcg
     1201 ccctggccgg tggcacggga cggcgtgtcg gtgtcgcgct cgccgccggg ctgcgcgccg
     1261 aggctgtcgg gctccggctg gctgtacagc ggcgcgccct cgccgccgcc ccccgcggtc
     1321 acgtctccgt cgccgccgtc ggccaagccg ccggcgccgg gctgggtgcc gtcgtcgccg
     1381 cgggtgcggg tcggcgtctc gtcacgctgg gagctcttgt cctcggggtc gtcgcggtcg
     1441 acggcgggga tggggaggga cgtgccggcg agcgcgggag ccgcgtacga ggtgttccac
     1501 acgcggtcga cgtcgcttgc ggcggagtag cgcacgcttc cgtcgggctg ctgcatcgag
     1561 gcaaggtagt ccagcggcgt cctgcccgac cccgcgccct tgaaggagcg ggcgctcacc
     1621 cgggccgcgg cgagcccgcg cagcacccag gaggtcgagg ccgagttgga ccccgcgcct
     1681 gcgttcccgt cgccgaagcc gccgtctgcg ttctggcgcg agcgcaggta ctccagcgcg
     1741 ccggcctggg cggccgtgtt gcgcctgccg ccggcgttga gggcctccag caccgacccg
     1801 gtcaggtcgg tgccgcccca cgatccgtcc ggcgactggc gttcctgcaa ccagtccagc
     1861 gcgcgggcct ggctgcgtcg cagctgggtc gcgctgaccc cacgcacgcg gctcgggtcg
     1921 atgccggcca gggcgagtac cgcgtaggcg gacccgttga tggtcgagcc cgggatgtcg
     1981 gggggctggg gaaagccccc gtcgcggcgc tggaggcgga gcaggcgccc cagcgggtcg
     2041 atcggcccga accgctgcgg cgaggtgccc gcggccgtgg ccaccaggat gacccgggcg
     2101 aagtccgtgc tctgcgtcag tccccgcgtg tggcgggtga ggtaggtgta gacgtccacc
     2161 ccgcccggct tgcgctggtc gcgcgggttg atgccgttgg aggccagcgc gagcgccgcc
     2221 cacgtgctga accccgggtc cgacccccgc tcgccgaacc caccgtcctt gtgctggacc
     2281 tcctgcaggt accggaccgt gctctccagg cgctgacgct cctgggcccc ggcggcggcc
     2341 gcgggcagca gcaggaacgc agccagcgcg accgcgctaa ggcgacgcag cgatgccatc
     2401 gagatcgatg ctcccccggg tcacgctgac ggtcacggta cccgcacggc ggcgcttcga
     2461 ccccagcacg gtccgccggc cgggccggac tcggacgctc ttggcgccga tgcgcacgac
     2521 ggccgccctg cgcccgcgga ccaggagggc cggccggccg gcggcgagct tcacccggat
     2581 ccgcgccccg gggcggcccc gtagcagcgt gcccttccag gccttgccgg agcggagctt
     2641 gcgccacggg cccgtgcgcc tgacgctctt ggcgcgctcg tccacgggga cgatgacgcg
     2701 gccgaagtcg atcgtgtcct ggcgcagcat ccggtcggtc gacgtgaagc gcagcgcgtg
     2761 gaccctgccg gcgggcagcc gcagcgacgc ggaggtcgcg ctggtgccgc gggcgcgggt
     2821 cacgtagcgc ttgctcctgc tggtcaggtc gtcggaggcg accgcccagc gcagcagccc
     2881 gacgccctgc tcggccacgc gccagcgcac cacgatcctc ccgcggcggt agcccgaggt
     2941 gccagccgcc gggcgcgtga tgcgcacctt ggcggtggtc ccggccaggg ccgggctgtc
     3001 acgcagtacc gcgccgccgg ggccgtagaa gaccggcgcg ggcggcaccc ggacctgggc
     3061 gtcggcggga gccggcccac aagcccccac gcagacgtag atccggttcg agcggatgaa
     3121 gcgggcgccg tcggccttga ggcggtacca gccgtcgccg gggaacgtga ccgagaagct
     3181 gccgctgccg ccggtgatcg tggcggccgg gctggaggtc aggccggcct cggcgatcgg
     3241 cttcggcgag ccgccgctct gcgtgacctg gaagccctgg gccacacccg gtgtcgccgt
     3301 ccccggcccg gcgagaccca ggaagggccg gccgctgaac gcgtcgtacg cccacaggac
     3361 ctcggcgttc tcgccgagcg cggtctggca gccgccgacg ctggtcaggt tgtcgccgac
     3421 cagcacgccc cagtaggcgg gccccgtctc gaggtccggc ccgaagccgc ggacgaagta
     3481 gtcctcgatg ttggcgttcc agcggccctc gaagccctgg ccgacgatgc tcatcgcgtc
     3541 cacggccgag gcggtgggcg tggccacgcg gcccgggttc gcgccgttgt tggtgccgtc
     3601 gcagtggtgg atcgtgccgg tggcacgctc ggacaccgcc tggacgttgc gcccggtggt
     3661 ctggaccggg ccctcgaaaa gggtggtgct acgaccctcg atgcgcacgt tcacgaccgt
     3721 ggccgcggcc ggggaggatg cggtgagcgc caccgcggcg acaaccatcg tcaagagctt
     3781 tcgcatgtac ctacggcccc tttccgcgag ggctgtcgag atgcggaccg gagcagatct
     3841 cctggctttc gagcacttcc gccacgcctt cccgggtgct tcccagtggc tggccccgtg
     3901 ggcccggggc ggaagtcctg ctcgatcaca gtggcgggcc cgcgccggtc ttgcaccggc
     3961 ttccctgacc accgatccgt ggactgtgcc gacccactct agcccctgcc ggccggtggg
     4021 gtgggtggcg ccccggggac cgcgggtgta cggtgctcgc actcacggcg cggggaacac
     4081 cggtgtgaat ccggggctga cgcgcagcgg tatgggtgac gaccggatcg cggtaccact
     4141 gggccacagg cccgggaagg agatccggaa ggtcgatccc gagcccgaac atccggccgt
     4201 gggaacaccc gtctcgcgtc taggcggcaa tgcaccttcg tgtgtcgccg cccctaccaa
     4261 ggagcacgac acatggcccg aacaccctca gcacggctca ccacggccgc ctgcctgctc
     4321 gcgctggcga tcacgcccgc gtccgcccac gccacgtcca gcggggccga catccgggcg
     4381 tcggtctccg acggagcgga ctgggcccag accaggcaga gcaccacgac cggcgccttc
     4441 ccggtgttcg gcttcgacat cgtgcccacc gccctggccg cggcgggccg caacgccgcc
     4501 gacgtgtccc gcggcggcgg gccgacctcc caggcctacg tggccggcgc gctgcagtcc
     4561 ctggcctacg acccctcggc cgcgtcgacg gtcggggaca tcgagcaggg catcctcagc
     4621 ggatacgccg cgggcatcga cccggcgcgc gtgagcgccg ggaagaacgt cgtggccgag
     4681 ctggccggcg cgtacgacga cggtcgctac gggccccggt tcagcttcaa caacaccgtc
     4741 tacggagcgc tggcgctgaa ccgcgtccgc gctccccggt tcctggtcga ccggacggtg
     4801 gccgccatcc gggccaacca gcacaacgac ggcggctgga gcttcaccga gtccgtgacc
     4861 cccgccgacc aggccaaccc cagcgccacc gacaccaccg gccccgcgct ggcggcactg
     4921 tgcgagtcgg gcgtcccggc gaacgacccc gccgtcgagc aggccgtgtc gttcctgcgc
     4981 tccaagctcg acctgggcac cggcggcttc gacccgcagg acggcttcgg cacgccgccc
     5041 aacgccgaca ccaacggcgc ggtgatctcg gggctcaacg cctgcggcat cgacccccag
     5101 ggcccccagt tcacgggccc gggcggccag acaccggtcg acttcctgat cggactccag
     5161 ctgaccgccg gggccgacgc cggcgggttc ccgtccgcgt tctcgggcgc gtcgggtgac
     5221 gtgtacgcga cccaggacgc ggtgcgcgca ctggccggag ccgccttcac ggcggcaccg
     5281 cccgcccgca ccacgcccac cgacccgcgc acccgccccg tgcccgccgt ggccgacggc
     5341 accagcgttc ccgtggccct ggccgtggac gacggctccg gcggcgtcac gttctgccgc
     5401 gtgaccgtgc ccagcggagc ctccctggcc acgctgctgg cgaccgcgaa cggctcgagc
     5461 tcgcccgcgg gctgcgccac cggcggccag ttcaccgcgg gccaggtcag caggctcaac
     5521 gggcagaccg gcacgtgggc
//
LOCUS       DASUPW010000273         2378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1586374, whole genome shotgun sequence.
ACCESSION   DASUPW010000273 DASUPW010000000
VERSION     DASUPW010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586374"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2378
                     /locus_tag="VFY44_06270"
     CDS             <1..>2378
                     /locus_tag="VFY44_06270"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEX5910080.1"
                     /translation="ATMDPARRAFYEYHSLLMEPWDGPAAVSFTDGSLVGATLDRNGL
                     RPGRYWITEDGLVVFASEVGVIDVEPSKVVKKGRVSPGKMFLVDTDAGRIIDDAEVKA
                     EVAAANPWADWVKDNLIDLKDLPEREHVVHTAASVNIRQRTFGYTTEELKILLGPMSR
                     TGAEPLGAMGSDTPVAVLSKRPRLLFDYFVQSFAQVTNPPLDAIREELVTSLTCAIGP
                     NGNLLDTKQVRQPQVSLPFPVINNDQLAKIANIENADGDKVAMKVRGLYRPEGGENAL
                     RARLTEICEQVSGAINRGVQYVVLSDRDSNAQWAPIPSLLLVSAVHHHLLRSANRTKT
                     ALVVEAGDVRETHHVAVLIGYGASAVNPYLAMESVEQLISTGDVLGVTPQDGVYNLIK
                     GLGKGVLKIMSKMGISTVASYTGAQTFEALGLGQDLVDEYFSGTHSQLGGVGLDVIAA
                     EVAARHRMAYPENGIEQPHQALMGGGEYQWRRDGEPHLFNPETVFRLQHATRERRYDI
                     FKSYTRGVDDQSENLMTLRGLLKFKAGLRPAVPLEEVESVSSIVKRFSTGAMSYGSIS
                     QEAHETLAIAMNRLGGKSNTGEGGEDVDRLLDPTRRSAVKQIASGRFGVTSLYLTNAD
                     DIQIKMAQGAKPGEGGQLMAQKVYPWVARTRHSTPGVGLISPPPHHDIYSIEDLAQLI
                     YDAKRANPSARVHVKLVSEVGIGTVAAGVTKAKADVVLVSGHDGGTGASPLNSLKHAG
                     VPWELGLAETQQTLQLNGLRDRVTLQVDGQLKTGRDVVIAALLGAEEFGFATA"
BASE COUNT          403 a          875 c          762 g          338 t
ORIGIN      
        1 gccacgatgg atccggcccg ccgcgccttc tacgagtacc actcgctgct gatggaaccg
       61 tgggacggcc ccgccgccgt gtccttcacc gacggcagcc tggtcggcgc caccctggac
      121 cgcaacggcc tgcgccccgg acggtactgg atcaccgagg atggcctggt cgttttcgcc
      181 tccgaggtcg gcgtgatcga cgtcgaaccc tccaaggtgg tcaagaaggg ccgcgtctcg
      241 cccggcaaga tgttcctcgt ggacaccgac gccggccgga tcatcgacga cgccgaggtc
      301 aaggccgagg tggccgcggc caacccgtgg gccgactggg tcaaggacaa cctgatcgac
      361 ctcaaggacc tgcccgagcg cgagcacgtg gtgcacaccg cggcgtcggt caacatccgc
      421 cagcgcacct tcggctacac caccgaggaa ctgaagatcc tgcttggccc gatgtcccgc
      481 accggcgccg agccgctcgg cgcgatgggc tcggacaccc ccgtggccgt gctctcgaag
      541 cggccccggc tgctgttcga ctactttgtg cagtccttcg cgcaggtcac caacccgccg
      601 ctggacgcca tccgcgagga actcgtcacg tcgctgacct gcgcgatcgg gcccaacggc
      661 aacctgctgg acaccaagca ggtccgccag ccgcaggttt cgctgccgtt ccccgtgatc
      721 aacaacgacc agctcgccaa gatcgcgaac attgagaacg ccgacggcga caaggtcgcc
      781 atgaaggtcc gcggcctcta ccgccccgaa ggcggcgaga acgcgctccg cgcccggctg
      841 accgaaatct gcgaacaggt ctccggcgcc atcaaccgtg gcgtgcagta cgtcgtcctc
      901 tcggaccgtg actcgaacgc ccagtgggct ccgatcccgt ccctgctgct ggtcagcgcc
      961 gtgcaccacc acctgctgcg cagcgccaac cgcaccaaga ccgcgctggt ggtcgaggcc
     1021 ggcgacgtcc gcgaaacgca ccacgtggcc gtgctgatcg gctacggcgc ctccgccgtg
     1081 aacccgtacc tggccatgga atccgtggag cagctcatct ccaccggcga cgtcctcggg
     1141 gtcaccccgc aggacggcgt ctacaacctg atcaagggcc tcggcaaggg cgtcctgaag
     1201 atcatgtcca agatgggcat ctccaccgtg gcctcctaca ccggagccca gacgttcgag
     1261 gccctgggcc tcggccagga cctggtcgac gagtacttct ccggcacgca ctcccagctc
     1321 ggcggcgtcg gcctggacgt catcgcggcc gaggtcgcgg cacgccaccg gatggcgtac
     1381 cccgagaacg gcatcgaaca gccccaccag gccctgatgg gcggcggcga gtaccagtgg
     1441 cgccgcgacg gcgagccgca cctgttcaac ccggagaccg tgttccggct gcagcacgcc
     1501 acccgtgaac gccgctacga catcttcaag tcctacaccc gcggtgtgga cgaccagtcc
     1561 gagaacctga tgaccctgcg cgggctgctg aagttcaagg ccgggctgcg ccccgcggtc
     1621 ccgctcgagg aagtggagtc cgtctccagc atcgtcaagc ggttctccac cggcgcgatg
     1681 agctacggct ccatctcgca ggaggcccac gaaaccctcg cgatcgccat gaaccggctg
     1741 ggcggcaagt ccaacaccgg tgagggcggc gaggacgtgg accggctgct ggacccgacc
     1801 cgccgctcgg cggtcaagca gatcgcgtcc ggccgcttcg gcgtgacgag cctgtacctg
     1861 accaacgccg atgacatcca gatcaagatg gcgcagggcg ccaagcccgg cgaaggcggc
     1921 cagctgatgg cgcagaaggt ctacccctgg gtggcccgga cgcggcactc gacgcccggc
     1981 gtcgggctca tctccccgcc cccgcaccac gacatctact cgatcgagga cctcgcccag
     2041 ctgatctacg acgcgaagcg ggccaacccc tcggcgcggg tgcacgtcaa gctggtctcc
     2101 gaggtcggga tcggcacggt ggccgccggc gtcacgaagg ccaaggccga cgtcgtcctg
     2161 gtctccgggc acgacggcgg caccggcgcc tcgccgctga actcgctcaa gcatgccggt
     2221 gtgccgtggg agctgggcct cgccgagacg cagcagacct tgcagctcaa cggtttgcgc
     2281 gatcgcgtga cgcttcaggt cgacggtcag ctcaagaccg gccgcgatgt cgtgatcgcc
     2341 gcgctgctcg gggccgagga gttcggcttc gccaccgc
//
LOCUS       DASUPW010000274         4696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_617788, whole genome shotgun sequence.
ACCESSION   DASUPW010000274 DASUPW010000000
VERSION     DASUPW010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617788"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..1671
                     /locus_tag="VFY44_06275"
     CDS             271..1671
                     /locus_tag="VFY44_06275"
                     /inference="COORDINATES: protein motif:HMM:NF005102.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX5910081.1"
                     /translation="MASTTPQYVTQDTGGSDSDLQELAKRHLWMHFTRMSAYDDAEVP
                     IITRGEGCHVYDEAGKRYFDGLAALFCVNAGHGRTEIGEAMAEQVKELGFFTNWSYAH
                     PTAIKLAKRIADLAPGDLNRVFFTSGGSEAVESALKLCRLYHRKHGEAGRFKLIAREI
                     AYHGTTMGALSATGIPDLRAPFEPMLPGNAHAPNTNSYRWPEDRDPLWAAEQIEETIL
                     FEGPETVSAVILEPVQNAGGCFVPQEGYWQKVREICDKYGVLLISDEVICSWGRLGHY
                     FGAQRFDYQPDLITTAKGLTSAYSPMGAVIASDKVAEVFMEGTNMFTHGITFGGHPVS
                     AAAAMANLDIFENEDLCGRVRENEADFRGKLESLRELPIVGDVRGAGYFYGIELVKDQ
                     DTKESFDDDESEWLLRGFLSGALYDQGLICRADDRGDPVIQLSPPLIAGPEEFDEITG
                     ILHSVLAEASEKMAGR"
     gene            1697..3250
                     /locus_tag="VFY44_06280"
     CDS             1697..3250
                     /locus_tag="VFY44_06280"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HEX5910082.1"
                     /translation="MLTLRTLLEEIELGLATGEESADAPVRWVHITELPDPTPWLSGG
                     ELILTTGLQLGTPELQREFVRRLADHQLAGLGFGVGFGHDELPAALVEEAERQAFPVF
                     EVPYDLPFIAVTEKAFARLVNEQYEVLQRGIAIHKRLERLVIEERGLDELVRALAAAL
                     GGTVTVLDAQCQPMAHRTFRRELDDGVLEAVCEEVGRRQEAAITELVPEHPAVAGRSV
                     VLPVYTRGQGAPHAWLFAARDSGGLGDFERLILQQATTVVALELMRQRAMHDTERRLA
                     GDVLAEALTGRLAERELASRLRPFGIGDRAAVLVFAVDDPVAEAGELDRFLHDGGHGA
                     LVAPRENVLCAVVDAGEEGLEPVELAREARAVLGGSGGPPRAAASRTEAAGALRRSFN
                     EARIALEATAIANGDAPDVASFRDMGASQLLLNLQDDEALALYCHSVLGPLEEDGGEY
                     GEELIRSLEAFIEQNGQWEKAARQLFCHRHTLRYRIRRVEELTGRDLGSARDRIEFWL
                     ALRGRELVP"
     gene            3247..4329
                     /locus_tag="VFY44_06285"
     CDS             3247..4329
                     /locus_tag="VFY44_06285"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEX5910083.1"
                     /translation="MRVAVLGAGGIIAPAIVRDLAESEEVTHLKLLDLDEARARATGE
                     AHAPGAEVRRVDPDGGLGYMLSGVDVLVNSASYRVNLDAMRACLEAGCHYIDLGGLYH
                     LTTRQLELDDDFTTVGKLALLGMGSSPGKTNVMGVRAVEELGATPELMDVYAAGRDLD
                     PPDGPSYPYAPRTLVDELTEPPMALRDGEPTALDPMQDGGSYPFPEPIGEGSSIYTLH
                     SEVRTFGDSFGCADCSFRLSLAPAVLERVKSLVGAEEEAIAAAAAEAVPSSGKTVSVH
                     VVTARSNGRLVTVTAKTQGMEDWGIGGGIVSTAAPAAAAVRLLARGAVDAVGAMPPER
                     CVRPEDLFPELERRNCTFNVETTEAT"
     gene            4326..>4696
                     /locus_tag="VFY44_06290"
     CDS             4326..>4696
                     /locus_tag="VFY44_06290"
                     /inference="COORDINATES: protein motif:HMM:NF017072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910084.1"
                     /translation="MKVGVPTEIKPDEYRVSLTPDGVRELADHGHDVIVQAGAGLGSA
                     ITDADYEAQGARILPDAEAVFGEAEMVLGVKEPQPQEVAMLRPGHLLFTYLHLAPDPE
                     LTRGLCESGATCVAYETVEDA"
BASE COUNT          682 a         1657 c         1739 g          618 t
ORIGIN      
        1 cttgagatcg ggctgcccgg cctcgggccg gccggcgctg gcgctctcca ctggctcctc
       61 ctcctacggt cgagccgggc atcgcgcccg gcaagagccg aagctaagcc caccctctcc
      121 cggcgcgcaa gtggcgtttg ccccaatccg cgccggtgtc gttgtacaga atgtccaatc
      181 ggcgaccgcc ggacgcgcac gaatggccgg ttgcgagcgg tgcagggcgc agataccgtt
      241 gttcacgacg tctagtcgga ggagatgaac gtggcaagca ccaccccgca gtacgtgacc
      301 caggacaccg gcggatcgga cagcgacctg caggagctgg ccaagcgcca cctgtggatg
      361 cacttcacgc gcatgagcgc gtacgacgac gccgaggtgc cgatcatcac ccgcggcgag
      421 ggctgccacg tgtacgacga ggcgggcaag cgctacttcg acggcctggc cgcgctgttc
      481 tgcgtgaacg ccggccacgg gcgcaccgag atcggcgagg cgatggccga gcaggtcaag
      541 gagctgggct tcttcaccaa ctggagctac gcccacccca cggcgatcaa gctggccaag
      601 cggatcgccg acctggcgcc gggcgacctg aaccgcgtgt tcttcaccag cggcggcagc
      661 gaggccgtgg agtcggcgct gaagctctgt cgcctctacc accgcaagca cggcgaggcc
      721 ggccggttca agctgatcgc gcgcgagatc gcctaccacg gcaccaccat gggcgcgctg
      781 tcggccaccg gcatccccga cctgcgggcg ccgttcgagc cgatgctccc cggcaacgcg
      841 cacgcgccca acaccaacag ctaccgctgg cccgaggacc gcgacccgct gtgggcggcc
      901 gagcagatcg aggagacgat cctgttcgag ggccccgaga cggtgtcggc ggtgatcctg
      961 gagccggtgc agaacgccgg cggctgcttc gtgccccagg agggctactg gcagaaggtc
     1021 cgcgagatct gtgacaagta cggcgtgctg ctgatctccg acgaggtcat ctgctcgtgg
     1081 ggccgcctgg gccactactt cggcgcccag cggttcgact accagcccga cctgatcacc
     1141 accgccaagg gcctgacgtc ggcgtactcc cccatgggcg ccgtgatcgc ctcggacaag
     1201 gtggccgagg tgttcatgga gggcacgaac atgttcaccc acggcatcac gttcggaggc
     1261 cacccggtgt cggccgccgc ggccatggcc aacctggaca tcttcgagaa cgaggacctg
     1321 tgcgggcgcg tgcgcgagaa cgaggccgac ttccgcggca agctcgagtc gctgcgcgag
     1381 ctgccgatcg tgggcgacgt gcgcggcgcg ggctacttct acggcatcga gctggtcaag
     1441 gatcaggaca ccaaggagtc cttcgacgac gacgagtccg agtggctgct gcgcggcttc
     1501 ctgtccggcg cgctgtacga ccagggcctg atctgccgcg ccgacgaccg cggcgacccc
     1561 gtgatccagc tctcgccgcc gctgatcgcg gggccggagg agttcgacga gatcaccggg
     1621 atcctgcaca gcgtgctcgc ggaggcgtcc gagaagatgg cgggccggta acggaggaag
     1681 gcgccccggg gccgcgatgc tcacgctgcg cacgctgctg gaggagatcg agctgggcct
     1741 ggccacgggc gaggagtccg cggacgcgcc cgtgcgctgg gtgcacatca ccgagctgcc
     1801 cgacccgacc ccgtggctct cgggcggcga gctgatcctg accacgggcc tccagctggg
     1861 cacgcccgag ctccagcgcg agttcgtgcg gcggctggcc gaccaccagc tggccgggct
     1921 gggcttcggg gtcggcttcg ggcacgacga gctcccggcc gcgctggtcg aggaggccga
     1981 gcggcaggcc ttcccggtgt tcgaggtgcc ctacgacctg ccgttcatcg ccgtgaccga
     2041 gaaggccttc gcgcgcctgg tgaacgagca gtacgaggtg ctccagcgcg gcatcgccat
     2101 ccacaagcgc ctggagcggc tggtcatcga ggagcgcggc ctggacgagc tggtgcgggc
     2161 gctggccgcg gcgctgggcg ggacggtcac ggtcctggac gcgcagtgcc agcccatggc
     2221 ccaccgcacg ttccggcgcg agctcgacga cggcgtcctg gaggccgtgt gcgaggaggt
     2281 cggccgccgc caggaggcgg cgatcaccga gctcgtgccc gagcacccgg cggtggccgg
     2341 ccgctcggtg gtgctgccgg tctacacccg cgggcagggc gctccccacg cgtggctgtt
     2401 cgccgcccgc gactccggcg gcctcggcga cttcgagcgc ctgatcctgc agcaggcgac
     2461 caccgtggtg gcgctggagc tgatgcgcca gcgtgcgatg cacgacaccg agcggcggct
     2521 tgccggcgac gtgctggccg aggccctgac ggggcggctg gccgagcgcg agctggcctc
     2581 gcggctgcgc ccgttcggca tcggcgaccg ggcggcggtc ctggtgttcg ccgtcgacga
     2641 cccggtggcc gaggccggag agctggaccg gttcctgcac gacggcggcc acggggcgct
     2701 ggtggcgccg cgcgagaacg tgctgtgcgc cgtggtcgac gccggcgagg agggcctgga
     2761 gccggtcgag ctggcgcgcg aggcgcgcgc ggtcctgggg ggcagcggcg gcccgccgcg
     2821 cgccgcggcc agccggaccg aggcggccgg cgccctgcgg cgctcgttca acgaggcgcg
     2881 gatcgcgctc gaggccacgg ccatcgccaa cggggacgcg cccgacgtgg cgtccttccg
     2941 ggacatgggc gcgtcgcagc tgctgctgaa cctccaggac gacgaggcgc tggcgctgta
     3001 ctgccacagc gtcctgggcc cgctggagga ggacgggggc gagtacggcg aggagctgat
     3061 ccgctcgctc gaggccttca tcgagcagaa cggccagtgg gagaaggccg cccgccagct
     3121 gttctgccac cgccacacgc tgcgctaccg gatccgccgg gtcgaggagc tgaccgggcg
     3181 cgacctgggc agcgcccgcg accggatcga gttctggctc gcactacgag gaagagagtt
     3241 ggtgccatga gggtggccgt actgggagcc gggggcatca tcgcccccgc gatcgtccgc
     3301 gacctcgcgg agtccgagga ggtcacgcac ctcaagctgc tggacctcga cgaggcccgc
     3361 gcccgtgcca cgggtgaggc gcacgcgccc ggcgccgagg tcaggcgggt cgatcccgac
     3421 ggcggcctcg gctacatgct cagcggcgtc gacgtgctgg tgaactcggc cagctaccgg
     3481 gtgaacctgg acgccatgcg cgcctgcctg gaggccgggt gccactacat cgacctcggc
     3541 gggctctacc acctgaccac gcgccagctc gagctcgacg acgacttcac cacggtcggg
     3601 aagctggcgc tgctgggcat gggcagctcg cccggcaaga ccaacgtgat gggggtgcgc
     3661 gcggtcgagg agctcggcgc cacgccggag ctgatggacg tgtacgccgc cggccgcgac
     3721 ctggacccgc ccgacggccc cagctacccg tacgcgccgc gcacgctggt cgacgagctg
     3781 accgagccgc cgatggccct gcgcgacggc gagcccaccg cgctggaccc gatgcaggac
     3841 ggcggctcct accccttccc cgagccgatc ggtgagggct cctcgatcta cacgctgcac
     3901 tccgaggtgc gcaccttcgg cgacagcttc ggctgcgcgg actgctcgtt ccggctgtcg
     3961 ctggccccgg cggtgctgga gcgcgtgaag agcctggtcg gcgcggagga ggaggccatc
     4021 gccgccgccg ccgccgaggc cgtgccctcg tcgggaaaga ccgtgtccgt gcacgtggtc
     4081 accgcccgtt cgaacgggcg gctggtgacc gtgaccgcga agacgcaggg catggaggac
     4141 tggggcatcg gtggcggcat cgtgtccacg gccgcgcccg ccgcggccgc cgtgcggctg
     4201 ctggcccgcg gcgccgtgga cgcggtgggc gccatgccgc ccgagcgctg cgtgcgcccc
     4261 gaggacctgt tcccggagct cgagcgccgc aactgcacgt tcaacgtcga gacaacggag
     4321 gccacgtgaa ggtaggggtt cccaccgaga tcaagcccga cgagtaccgg gtgtcgctga
     4381 cgccggacgg cgtgcgcgag ctggccgacc acggtcacga cgtgatcgtg caggccggcg
     4441 cgggcctggg gtccgccatc accgacgccg actacgaggc gcagggcgcc cggatcctgc
     4501 ccgacgcgga ggcggtgttc ggcgaggccg agatggtgct gggcgtcaag gagccccagc
     4561 cgcaggaggt ggcgatgctc cggcccggcc acctgctgtt cacctacctg cacctggccc
     4621 ccgaccccga gctgacccgc ggcctgtgcg agtccggcgc cacctgcgtg gcctacgaga
     4681 ccgtcgagga cgccga
//
LOCUS       DASUPW010000275         2421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1865425, whole genome shotgun sequence.
ACCESSION   DASUPW010000275 DASUPW010000000
VERSION     DASUPW010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1865425"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..1668
                     /locus_tag="VFY44_06295"
     CDS             76..1668
                     /locus_tag="VFY44_06295"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5910085.1"
                     /translation="MRFSDIPRGSAQRTGMRPFAAGLIAVLVVGLGTFFAFTKANPFA
                     SPYELEAVFANANRVAERSPVRIAGVNVGEVTSVEPLEDGSGYARVTMEIDEQGLPIH
                     KDAQLKVRSRLFLEGNYFVELRPGTPGKPVLASGATIPPQQTAAPVQFNQVLTALQSE
                     TREDLRTLLKEYSSALKGPGARGFNQAIKHWEDAYRDGSQVADATRGQEAHDLSRLLR
                     GQGRVFGALSRNPQQLSELVTDLNDTIAGFARQEANLRATVPELRDVLREGRPALISL
                     NSALPSVRRFARDALPGARSSNATLDAQLPFIRQARALISERELRGLARKLQATLPPL
                     TRLNTRTPATLEQYRALASCQNRVLLPFAKEPIPDPSFPWADKQPFFKESSRALVGLS
                     GESRLADANSPYFRVLAGGGATTLVQTGESGQKLFSSALLPLDGVRPAKPPKRPVFRP
                     GVPCETQEPPDLNSTTGVGETAVNPATARLSSKQRAERDDGLEALREYTRDSRAGREA
                     VDPLVDPSVIGKDEKKKKEDEE"
     gene            1665..>2421
                     /locus_tag="VFY44_06300"
     CDS             1665..>2421
                     /locus_tag="VFY44_06300"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5910086.1"
                     /translation="MKRALRTHLRDMIALGAMVALGLGVAAYILSNQRLRFPLLEEKP
                     VPMWVELPNAQGVTPGQGQTVRVSGMRIGDIGKVELRGGRARVRMDVEPEYAGMVRRD
                     ASVLLRPRTGLKDMFLALDPGTRREPRLGRDGVIPIDNSVPDLNADEILAALDTDTRA
                     YLRLLINGAGKGLKGRDRDLREVFRRLGPLHRDLAALQGEVVKRRRNLARLVHNYGST
                     VDRLGREDRDLSTLVSGANRVFGRLSREDGNISE"
BASE COUNT          353 a          893 c          875 g          300 t
ORIGIN      
        1 tcgggatcga caacctgaag gacgttccat gagcgcaagc agccggctcg gccggatgcg
       61 cagcgaggat ctcctttgag gttctcggac atcccccgcg gtagcgccca gcgcaccggg
      121 atgcggccgt tcgcggccgg cctgatcgcg gtgctggtgg tgggcctggg cacgttcttc
      181 gcgttcacca aggccaaccc gttcgccagc ccctacgagc tggaggccgt gttcgccaac
      241 gccaaccgcg tggccgagcg ctcgccggtg cggatcgcgg gggtgaacgt cggcgaggtc
      301 acgtcggtcg agccgctgga ggacggctcg ggctacgcgc gcgtgacgat ggagatcgac
      361 gagcagggcc tgccgatcca caaggacgcc cagctgaagg tgcgctcgcg cctgttcctg
      421 gagggcaact acttcgtcga gctgcgcccc ggcacgccgg gcaagccggt gctggccagc
      481 ggagccacca tcccgcccca gcagacggcg gcgccggtgc agttcaacca ggtgctgacc
      541 gcgctgcagt ccgagacgcg cgaggacctg cgcaccctgc tcaaggagta ctccagcgcg
      601 ctcaagggcc cgggcgcgcg cgggttcaac caggcgatca agcactggga ggacgcgtac
      661 cgcgacgggt cccaggtggc cgacgccacg cgcgggcagg aggcccacga tctgagccgg
      721 ctgctgcgcg gccagggccg cgtgttcggc gccctttcgc gcaacccgca gcagctgagc
      781 gagctggtca ccgacctcaa cgacaccatc gccgggttcg cccgccagga ggccaacctg
      841 cgcgccaccg tgcccgagct gcgcgacgtc ctccgcgagg gccgcccggc gctgatctcg
      901 ctgaactcgg cgctgccctc ggtgcgccgg ttcgcgcgcg acgcgctgcc gggggcgcgc
      961 tcgtcgaacg cgacgctgga cgcccagctc ccgttcatcc gccaggcgcg ggcgctgatc
     1021 tccgagcgcg agctgcgcgg gctggcccgc aagctccagg ccacgctgcc gccgctgacc
     1081 cgcctgaaca cgcggacccc ggccaccctc gagcagtacc gcgcgctggc ctcgtgccag
     1141 aaccgcgtgc tgctcccgtt cgccaaggag ccgatcccgg acccgtcgtt cccgtgggcg
     1201 gacaagcagc cgttcttcaa ggagtcctcg cgcgcgctgg tggggctctc gggcgagagc
     1261 cgcctggccg acgccaactc gccctacttc cgggtgctgg ccggcggcgg ggccaccacg
     1321 ctggtgcaga ccggggagag cggccagaag ctgttctcca gcgcgctgct gccgctggac
     1381 ggggtgcgcc cggccaagcc gcccaagcga cccgtgttcc ggcccggcgt gccgtgcgag
     1441 acccaggagc cgcccgacct gaactccacc accggcgtcg gcgagaccgc ggtcaacccg
     1501 gccacggcgc gcctctcgtc caagcagcgc gccgagcgcg acgacggcct cgaggcgctg
     1561 cgcgagtaca cgcgcgactc gcgcgcgggg cgcgaggccg tggatccgct cgtggacccg
     1621 tcggtgatcg gcaaggacga gaagaagaag aaggaggacg aggagtgaag cgggcgctgc
     1681 gcacccacct gcgcgacatg atcgcgctgg gcgccatggt cgccctgggg ctgggcgtgg
     1741 ccgcctacat cctgtccaac cagcggctgc gcttcccgct cctggaggag aagccggtgc
     1801 ccatgtgggt cgagctgccc aacgcgcagg gcgtgacgcc cggccagggc cagaccgtgc
     1861 gcgtgtccgg catgcgcatc ggtgacatcg gcaaggtcga gctccggggc gggcgcgcac
     1921 gcgtgcgcat ggacgtggag cccgagtacg ccggcatggt ccgccgcgac gccagcgtgc
     1981 tgctgcgccc acgcaccggc ctgaaggaca tgttcctggc gctggacccc ggcacccggc
     2041 gcgagccgcg gctgggccgc gacggggtca tcccgatcga caactcggtc cccgacctga
     2101 acgccgacga gatcctggcc gcgctggaca ccgacacccg cgcctacctg cggctgctga
     2161 tcaacggcgc gggcaagggg ctgaaggggc gcgaccgcga cctgcgcgag gtgttccggc
     2221 ggctcgggcc gctgcaccgc gacctggcgg cgctgcaggg cgaggtggtc aagcgccgcc
     2281 gcaacctcgc ccgcctggtg cacaactacg gctcgaccgt cgaccgactg gggcgcgagg
     2341 accgcgacct gtccacgctg gtcagcggcg ccaaccgcgt gttcggccgc ctcagccgcg
     2401 aggacggcaa catctccgag g
//
LOCUS       DASUPW010000276         3799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_756543, whole genome shotgun sequence.
ACCESSION   DASUPW010000276 DASUPW010000000
VERSION     DASUPW010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756543"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VFY44_06305"
     CDS             complement(<1..275)
                     /locus_tag="VFY44_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910087.1"
                     /translation="MSGRARGRPARVEFPMVGVLGRRGRFPVAEPLFAPSGATVEVAR
                     GGGSEGDLVLVGGGKKGARVVRRLGRPDVARDVVEALMLDRGLRRSFP"
     gene            complement(272..1450)
                     /locus_tag="VFY44_06310"
     CDS             complement(272..1450)
                     /locus_tag="VFY44_06310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX5910088.1"
                     /translation="MSPLRKTLLLVVTCVLLLFGGIWLGGHPEALPGPVRDAFVDEDR
                     ALRAEIIDDVKDNFYKPVSDKQIEESSLKGIVDGLNDRFSSYLTPKETKQFQESVQGR
                     FEGVGMAVDQDKRGLRVINVFEGSPAKKAGIKKGDFVTEVDGKSIAGQSSSVATAKIK
                     GPAGTKVSLQIVDADKGTTETYKLERRRIEVPVAEGKVETRNGKKVGVVKLQSFSEGA
                     HGLLRRQIDKVRKQGATSLVLDLRGNGGGLLDEGVRVASIFIPKGLITYTEGRTSPRR
                     DFDATGGAIPKDIPVVVLVDRGSASASEIVTGALKDTKRATVVGTRTFGKGVFQQVLP
                     LSNGGVLDITVGEYFLPKGSNINGKGIQPSVKATDDPETERDEALPKALETVVGKPAG
                     "
     gene            complement(1525..2967)
                     /locus_tag="VFY44_06315"
     CDS             complement(1525..2967)
                     /locus_tag="VFY44_06315"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HEX5910089.1"
                     /translation="MPQAADGAPFTVNTTTDAAPVANACVSVPSCSLREAIVAANANP
                     DADVITVPGGTYTLTQGTLTVTNPVTIDGAGARSTTVDGNDASRVFTINNSGTITTIT
                     GLTVTGGNADATAPVTAGNGGGFLTGPQANLVLRGTAVVGNRASLSGGGISYGHTGAT
                     GTIESSLIAGNTAGDPASVMAANGGGIFAQGGLTVSNSTITDNSARTTLAAQGGGILA
                     LAMNVNLVNNTVSDNTAAGGTSMGGGIMNAATLSATNTIVAGNTGGDCGAAALGATLA
                     NNLSGDASCGFTGAAGTQNANPALGPLADNGGPTNTRAIAQSSPAANAGTATGCPATD
                     QRGVARPQGPACDIGAFEAPYTAPGAGGGAATPPVTPPRTSAGRKPGLKVTGVPKRCR
                     RSKFEIRLRSSVSGTGVKLRSVRVTLNGKKLRTVTKAGRFRVKIDAKKLKAGRKYTIR
                     VRATDSKGRATAFKKSFKRCKAKTAKRKKR"
     gene            complement(3122..>3799)
                     /locus_tag="VFY44_06320"
     CDS             complement(3122..>3799)
                     /locus_tag="VFY44_06320"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEX5910090.1"
                     /translation="ALEGSPNVKSVEFISKAEGLKQAKKKNPEAFAEGSKLLGGNPLP
                     DTFRIVPDDPDQVNAIVDKLAPRDASGDRVASLAGLDEVRNKESDTDKILSATGLVKI
                     LTAGLAALLIFASVALIANTIRLSIFARRREVEVMKLVGATNWFIRWPFVIEGVIVGF
                     MGGVLAVLLLTVAKETFIDPLQARFDLLAAPDTINFGLLIVVLMIACMGVSAAGSALT
                     LRRFLRV"
BASE COUNT          505 a         1345 c         1342 g          607 t
ORIGIN      
        1 gggaacgagc gccgcaggcc gcggtccagc atcagcgcct cgaccacgtc gcgggcaacg
       61 tcggggcggc ccagccgccg gaccacccgc gcgcccttct tgccgccgcc gaccaggacc
      121 aggtcgccct cggaccctcc cccgcgcgcc acctcgacgg tggccccgga aggcgcgaag
      181 aggggctcgg ccaccgggaa ccgcccccgg cgtcccagca cgccgaccat cgggaactcc
      241 acgcgcgcgg ggcgcccccg cgccctgccg ctcatccggc gggcttgccg accacggtct
      301 ccagcgcctt gggcagcgct tcgtcccgct cggtctccgg atcgtcggtg gccttgaccg
      361 acggctggat gcccttgccg ttgatgttcg agcccttggg caggaagtac tcgcccacgg
      421 tgatgtccag gacgccgccg ttggacagcg ggagcacctg ctggaacacg cccttgccga
      481 acgtgcgtgt gccgaccacc gtcgcccgct tggtgtcctt cagggcgccg gtcacgatct
      541 cggacgccga ggcgctgcca cggtccacca gcaccacgac ggggatgtcc ttggggatcg
      601 ccccgccggt ggcgtcgaag tcgcgccgcg gcgaggtgcg gccctcggtg tacgtgatca
      661 ggcccttggg gatgaagatc gaggccacgc gcacgccctc gtccagcagc cctccgccgt
      721 tgccgcgcag gtccagcacc agggaggtcg cgccctgctt gcggaccttg tcgatctggc
      781 gccgcagcag gccgtgggcg ccctcgctga agctctggag cttgaccacg ccgaccttct
      841 tgccgttgcg cgtctcgacc ttgccctcgg ccaccgggac ctcgatccgc cggcgctcga
      901 gcttgtaggt ctcggtggtg cccttgtcgg cgtccacgat ctgcagcgag accttggtgc
      961 ccgccgggcc cttgattttc gccgtggcca cggagctgga ctggccggcg atggacttgc
     1021 cgtccacctc ggtcacgaag tcgcccttct tgatgcccgc cttcttcgcg ggcgagccct
     1081 cgaacacgtt gatgacccgg agtccccgct tgtcctggtc gacggccatg ccgacgccct
     1141 cgaaccggcc ctggaccgac tcctggaact gcttggtctc cttgggcgtc aggtaggacg
     1201 agaagcggtc gttcagcccg tcgacgatgc ccttgagcga gctctcctcg atctgcttgt
     1261 cgctgaccgg cttgtagaag ttgtccttga cgtcgtcgat gatctcggcg cgcagggcac
     1321 ggtcctcgtc cacgaaggca tcgcgcaccg gccccgggag ggcctcgggg tgcccgccga
     1381 gccagatgcc gccgaacagc agcagcacgc aggtcacgac cagcagcagg gtcttacgca
     1441 gtggactcat ggaaagaggc cgcacgcgcg gcctcggcta cagggtagag agcggcgggg
     1501 ccggggcccc gccgcccgtc ggaatcagcg cttcttgcgc ttcgccgtct tcgccttgca
     1561 gcgcttgaac gacttcttga aggccgtggc acggcccttg ctgtccgtcg ccctgaccct
     1621 gatcgtgtac ttcctgccgg ccttcagctt cttggcgtcg atcttcacgc ggaagcggcc
     1681 ggccttcgtc accgtcctga gcttcttgcc gttcagcgtc acgcgcaccg agcggagctt
     1741 cacgccggtc ccggagaccg acgagcgcag ccggatctcg aacttcgagc gccggcagcg
     1801 cttcgggacg ccggtgacct tcaggccggg cttcctgccc gccgaggtac gtggcggggt
     1861 cacgggcggc gtggcggcac cgccacccgc accgggggcc gtgtagggcg cctcgaaggc
     1921 gccgatgtcg cacgccggcc cctggggccg ggcgacgccg cgctggtccg tggccgggca
     1981 gccggtggcg gtgcccgcgt tggcggccgg gctggactgc gcgatcgccc gtgtgttggt
     2041 ggggccgccg ttgtcggcca gcgggccgag tgccggattc gcgttctgcg tcccggccgc
     2101 cccggtgaag ccgcaggagg cgtcgcccga gaggttgttg gccagcgtcg cccccagggc
     2161 cgctgcgccg cagtcgcccc ccgtgttgcc cgccacgatg gtgttggtgg cgctcagggt
     2221 ggccgcgttc atgatgccgc cgcccatgga tgtgccgccc gccgcggtgt tgtcgctgac
     2281 cgtgttgttc accaggttca cgttcatcgc gagggccagg atgccgccgc cctgtgcggc
     2341 gagcgtggtg cgcgcggagt tgtcggtgat cgtcgagttc gagaccgtca ggccgccctg
     2401 ggcgaagatc ccgcctccgt tggcggccat caccgaggca gggtcacccg cggtgttgcc
     2461 ggcgatgagc gagctctcga tcgtgccggt ggcaccggtg tggccgtagg agatcccacc
     2521 gccgctgagc gaggcgcggt tgcccaccac ggccgtgccg cgcagcacga ggttggcctg
     2581 cgggcccgtc aggaacccgc ccccgtttcc tgcggtcacc ggggcggtgg cgtcggcgtt
     2641 gcccccggtc accgtcaggc cggtgatcgt cgtgatcgtg ccggagttgt tgatggtgaa
     2701 gacgcggctg gcgtcgttgc cgtcgacggt ggtcgaccgc gccccggcgc cgtcgatcgt
     2761 gaccgggttg gtcaccgtga gcgtgccctg ggtcagggtg taggtgccgc ccgggaccgt
     2821 gatcacgtcg gcatcggggt tggcgttggc cgccacgatg gcctcgcgca gcgaacagga
     2881 cggaacgctc acgcaggcgt tggccaccgg cgccgcgtcg gtggtggtgt tcacggtgaa
     2941 cggcgcgccg tccgcggctt gcggcacggc gagacacgac agggcgagca ggagcggcag
     3001 gagacgacgg gaacgcaaag gacttccttc caacggggcc aggacagggc agcctggccc
     3061 cccgccgagc gccgcccggt cccaatgctc cccggttccg cgcccgggga aacccgaacg
     3121 ctcagacgcg caggaaacgc cgcagcgtca gcgcgctgcc ggcggccgag acgcccatgc
     3181 aggcgatcat caacaccacg atcagcaggc cgaagttgat cgtgtccggc gcggccagca
     3241 gatcgaagcg ggcctggagc ggatcgatga acgtctcctt ggccacggtc agcagcagca
     3301 cggccaggac accgcccatg aagcccacga tcacgccctc gatcacgaac ggccagcgga
     3361 tgaaccagtt ggtggccccg accagcttca tcacctccac ctcgcggcgg cgggcgaata
     3421 tcgacagccg gatggtgttg gcgatcaggg cgaccgaggc gaagatcagc agcgcggcca
     3481 ggccggcggt caggatcttg accaggccgg tggccgacag gatcttgtcg gtgtccgact
     3541 ccttgttgcg gacctcgtcc aggccggcca gcgaggccac gcggtcaccc gaggcgtcgc
     3601 gcggggcgag cttgtcgacg atcgcgttca cctggtcggg gtcgtcgggg acgatccgga
     3661 aggtgtcggg cagcgggttg ccgcccagca gcttggagcc ctcggcgaag gcctcggggt
     3721 tcttcttctt ggcctgcttc agcccttcgg ccttggagat gaactcgacc gacttcacgt
     3781 tgggcgagcc ctccagcgc
//
LOCUS       DASUPW010000277         2408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1450194, whole genome shotgun sequence.
ACCESSION   DASUPW010000277 DASUPW010000000
VERSION     DASUPW010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450194"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VFY44_06325"
     CDS             <1..309
                     /locus_tag="VFY44_06325"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX5910091.1"
                     /translation="KDDSAGGGKTQAQSGGFKKVAKVDIRDFKYRPPKDEVKVGQSIK
                     FTNQDTAKHTATSQPQGTFDSGDLEKGQTKPVKFTKAGTYTYYCVYHAFMKGKVKVVG
                     "
     gene            306..698
                     /locus_tag="VFY44_06330"
     CDS             306..698
                     /locus_tag="VFY44_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910092.1"
                     /translation="MTTDALRYVAAALVLVVAGVHWQQYVELLNQVDTVGPLFLLNAA
                     GGAALAVLLLQRDVTLRILGALGAIPLCLGSLISIVLSMGDGIFGYQEPDWRAAVVVA
                     VVAEVAALPVLLAYLARTYTARQWDGAR"
     gene            680..>2408
                     /locus_tag="VFY44_06335"
     CDS             680..>2408
                     /locus_tag="VFY44_06335"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEX5910093.1"
                     /translation="MGRRALAAVAIAVIGLVAPAAARAAPPSSCGLPGPDQATTGGFD
                     AARQGSFVFVPFDVPAGTTQVRVAYCWDGSQGTSDHTLDLGVYQARGDTRRPWGGADF
                     RGWGGSGYRDVTITPQGFSSDAQYQADPKAYTPGRTTRSFVPGPIPAGRWAAELGLAN
                     IDPTDLDGVAYRVEVKYYRDRAFAATPYRRPRHDPAPVRAGTAWYAGDFHVHAEHSGD
                     ARASFAKTLGYAFRPRPAGGPGLDFVAVTDHNTGTGWPEEDRLRGEYPGRLILRGEEV
                     TTYNGHTNNIAGGVEPDYRTGPLYERQGDGSLRLVRAAQPVSGVFDTVNRAGGITQIN
                     HPTLFPSPPFPSNLCRGCQWTHSDAATDYSKVDSIEVATGPQRVSAAPNPFVATAIGF
                     WDDKLRRGHHIAAVGGSDDHRASGGTGPTDAPVGQPTTMVRACGLSEQSVRQAVEAGR
                     TYVKVGGPGAPDLDLTGRIRGTRGPEAIIGDTIHSAAPVDFRATVRGAAGVSVLQVVK
                     DGNVIRAVPLSGPAATVDFDSTGPGYYRLQVNEGQFVSAVSSPIYVVGQAGPPPSGGA
                     CPPARTRLRA"
BASE COUNT          335 a          900 c          865 g          308 t
ORIGIN      
        1 aaggacgact cggccggcgg cggcaagacg caggcgcagt cgggggggtt caagaaggtc
       61 gcgaaggtgg acatcaggga cttcaagtac cggccgccca aggacgaggt caaggtgggc
      121 cagagcatca agttcaccaa ccaggacacg gccaagcaca ccgccacgtc ccagccccag
      181 ggcacgttcg attcgggtga cctcgagaag ggccagacca agccggtcaa gttcaccaag
      241 gccgggacct acacgtacta ctgcgtctac cacgcgttca tgaagggcaa agtcaaggtg
      301 gtcgggtgac caccgacgcg ctccgctacg tggccgccgc gctcgtgctc gtggtggccg
      361 gcgtgcactg gcagcagtac gtcgagctcc tgaatcaggt cgacacggtc ggcccgctgt
      421 tcctgctgaa tgcggccggc ggggccgcgc tggcggtcct gttgctgcag cgcgacgtga
      481 ccctccggat cctcggcgcg ctgggcgcga tcccgctctg cctgggctcg ctgatctcga
      541 tcgtgctgtc gatgggcgac gggatcttcg gctaccagga gcccgactgg cgcgccgcgg
      601 tggtcgtcgc ggtcgtggcc gaggtggctg cgcttccggt gctgctggcc tacctggcgc
      661 ggacgtacac tgcccgccag tgggacggcg cgcgctagcg gcagtcgcga ttgcggtcat
      721 cggcctggtt gcgcccgcgg ccgcgcgggc ggcgccgccg tcgagctgcg gcctgccggg
      781 tcccgatcag gcgaccaccg gcggcttcga cgccgcgcgc cagggcagct tcgtgttcgt
      841 gcccttcgac gtgcccgccg gcaccacgca ggtgcgcgtg gcctactgct gggacggctc
      901 ccagggcacc agcgaccaca cgctggacct gggcgtctac caggcgcgcg gggacacccg
      961 gcggccgtgg ggcggggccg acttccgcgg ctggggcgga tcgggctacc gcgacgtcac
     1021 gatcacgccg cagggcttct ccagcgacgc gcagtaccag gccgacccga aggcctacac
     1081 gcccgggcgc accacgcgct cgttcgtgcc cggcccgatc ccggccgggc gctgggccgc
     1141 cgagctgggc ctggcgaaca tcgaccccac cgacctggac ggcgtcgcct accgcgtcga
     1201 ggtcaagtac taccgcgacc gcgcgttcgc ggcgaccccc tatcgccggc cgcggcacga
     1261 cccggccccg gtgcgcgcgg gcacggcctg gtacgcgggt gacttccacg tgcacgccga
     1321 gcactcgggc gacgcccggg ccagcttcgc caagacgctc ggctacgcgt tccggccccg
     1381 gccggccggg ggcccggggc tggacttcgt ggccgtgacc gaccacaaca ccggcaccgg
     1441 ctggcccgag gaggaccgcc tccgcgggga gtatccgggc cggctgatcc tgcgcggcga
     1501 ggaggtgacc acctacaacg gccacaccaa caacatcgcc ggcggggtcg agcccgacta
     1561 ccgcaccggc cccctctacg agcgccaggg cgacggctcg ctccggctcg tgcgcgcggc
     1621 ccagccggtg tcgggggtgt tcgacaccgt caaccgggcg ggcgggatca cgcagatcaa
     1681 ccacccgacc ctgttcccct cgccgccgtt cccgtcgaac ctgtgccgtg gctgccagtg
     1741 gacccactcg gacgccgcca ccgactactc gaaggtcgac tcgatcgagg tggccaccgg
     1801 cccccagcgg gtcagtgccg cgcccaaccc gttcgtggcc accgcgatcg ggttctggga
     1861 cgacaagctg cgccgcgggc accacatcgc cgcggtgggc gggagcgacg accaccgcgc
     1921 gtcgggggga accggtccga ccgacgcgcc cgtgggtcag ccgacgacga tggtgcgcgc
     1981 ctgcggcctg tccgagcaga gcgtgcgcca ggccgtcgag gcgggccgca cctacgtcaa
     2041 ggtcggcggc ccgggcgcgc ccgacctgga cctgaccggg cgcatacgcg gcacgcgcgg
     2101 gccggaggcg atcatcggcg acaccatcca ctcggccgcc ccggtcgact tccgtgccac
     2161 ggtcaggggc gccgccgggg tgtcggtgct gcaggtggtc aaggacggca acgtcatccg
     2221 ggcggtgccc ctctcggggc cggcggccac cgtcgacttc gactcgaccg gccccggcta
     2281 ctaccgcctg caggtgaacg agggccaatt cgtcagcgcc gtctccagcc cgatctacgt
     2341 ggtcgggcag gccggcccgc cgcccagcgg cggcgcgtgc ccgcctgcgc gcacccgcct
     2401 gcgcgccg
//
LOCUS       DASUPW010000278         2726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1727356, whole genome shotgun sequence.
ACCESSION   DASUPW010000278 DASUPW010000000
VERSION     DASUPW010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1727356"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..2163
                     /locus_tag="VFY44_06340"
     CDS             271..2163
                     /locus_tag="VFY44_06340"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5910094.1"
                     /translation="MAEYRPKNQGLERVLGTGALFSTAYGNVGSSIYYALGLVAVFAL
                     GMTPVVFLIAGIIFVLTAITYTEATTMYAEAGGSSSFARHAFNETVSAVTAWGQMLNY
                     IITVAISAFFLPHYLGVFWEPLKSSPADVIVGITVVVVLAAVNVVGVQEAAKLNIFLA
                     IMDLVTQVGIVILGAVLVLSPETLVNNVDFGTSPKLTDFLIAIPVGMVAFTGIDTISN
                     MAEEARDQAKTIPAAMKGVVAAVMAISLTLPAVALSAMPVENGSTPLGEPSDQGGYAG
                     DPITGLVVNLGLGDAAEGFLKGYVGLLAATILLVATNAGLIGLSRLSYSMGQYRQLPE
                     KLRQLHPRFRTPYVAIVVFGAIACLTMVPGQADFLGTVYAFGAMLSFTVAHASVIALR
                     IRQPDAERPFRGPGNLKVRGYDLPLFAIVGALGTGAAFIAVTALNTVVLVFGGLWLAM
                     GMTVYYTYRRHLGLPARETRKIITPDPIVEHEVEYESILVAFEDAEYSTEAVAMAVRL
                     AARRRRGIHVLVTITVPANAPIDASMPDAEAKAQAAVDRARVRGGRRVTGHYEKVRAG
                     GAGRRIVSEAREIHAAAIVMPLPRKRAGSSLFGRTLETVLAERPCRVIIDSSLGMAKS
                     GDKAAA"
     gene            2163..2363
                     /locus_tag="VFY44_06345"
     CDS             2163..2363
                     /locus_tag="VFY44_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910095.1"
                     /translation="MERFLGASSLVLGTLLAVLGVVMVVSTLARGGGPLAFGVILGVL
                     FVLAGAGRLYLAGVRPRRGGSR"
     gene            2360..>2726
                     /locus_tag="VFY44_06350"
     CDS             2360..>2726
                     /locus_tag="VFY44_06350"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX5910096.1"
                     /translation="MNAEGRVARGLGQPALFAIGISAVASSLYYVLGIVAGDAQGLTP
                     LVFAVGALFFVLTVLTYVEGSSLHPERGGASVLARYAFNELWSFIAGWAILLDFLIVL
                     AIGAVAVPHYLSAFWGPADE"
BASE COUNT          361 a          944 c          980 g          441 t
ORIGIN      
        1 cgcgaagaag ctgatcgagg acggcgagtt cggcggcaag ggctcggagg cccacgaggc
       61 ctcgatcacg ggcgacacgg tcggtgaccc ctacaaggac accgccggcc cggcgatcaa
      121 cccgatgatc aaggtcgcga acatcgtggc catcctgatc atccccttcc tggtctaggc
      181 cccggcggca caccaaggtt gagcgagggg cccggcatca cgccgggccc ctcgtcgttc
      241 tgggcacagg agtagctttc tcgctcggcc atggccgagt accgccccaa gaaccaaggg
      301 ctcgagcgcg ttctgggtac cggggcgctc ttcagcaccg cctacggcaa cgtgggcagc
      361 tcgatctact acgccctcgg gctggtggcc gtcttcgccc tgggcatgac gcccgtggtc
      421 ttcctgatcg cgggcatcat cttcgtgctc acggcgatca cctacaccga ggccacgacg
      481 atgtacgccg aggccggcgg gtcctcgtcg ttcgcgcgcc acgccttcaa cgagaccgtc
      541 tcggcggtca cggcgtgggg gcagatgctc aactacatca tcacggtcgc catctcggcg
      601 ttcttcctcc cgcactacct gggcgtgttc tgggagccgc tgaagagcag cccggcggac
      661 gtgatcgtgg gcatcaccgt ggtcgtggtg ctggccgcgg tgaacgtcgt gggcgtgcag
      721 gaggcggcga agctgaacat cttcctggcg atcatggacc tggtcaccca ggtgggcatc
      781 gtgatcctgg gcgcggtgct ggtgctctcg cccgagacgc tggtcaacaa cgtcgacttc
      841 ggcacatcgc cgaagttgac cgacttcctg atcgcgatcc cggtggggat ggtggccttc
      901 accggcatcg acacgatctc gaacatggcc gaggaggccc gcgaccaggc caagaccatc
      961 ccggccgcga tgaagggcgt ggtggccgcg gtgatggcga tatcgctgac cctgcccgcg
     1021 gtcgcgctgt cggcgatgcc ggtcgagaac gggtcgacgc cgctgggcga gccatcggac
     1081 cagggcggct acgccggcga cccgatcacc ggcctggtgg tgaacctggg cctgggcgac
     1141 gcggccgagg gcttcctgaa gggctacgtg gggctcctgg ccgccacgat cctgctcgtg
     1201 gccaccaacg cgggcctgat cggcctgtcg cggctgagct actcgatggg ccagtaccgc
     1261 cagcttcccg agaagctgcg ccagctgcac ccgcgcttcc gcacgccgta cgtggccatc
     1321 gtggtcttcg gcgccatcgc ctgccttacg atggtcccgg gccaggccga cttcctgggc
     1381 acggtctacg cgttcggcgc gatgctgtcc ttcaccgtcg cgcacgcctc ggtgatcgcg
     1441 ctgcgcatca ggcaacccga cgccgagcgg ccgttccggg ggccgggcaa cctcaaggtc
     1501 cgcgggtacg acctgccgct gttcgccatc gtcggcgcgc tgggcacggg cgcggcgttc
     1561 atcgcggtga ccgcgctgaa cacggtcgtg ctggtcttcg gcggcctctg gctggccatg
     1621 ggcatgaccg tctactacac ctaccgccgc cacctgggcc tgccggcacg cgagacgcgc
     1681 aagatcatca cgcccgaccc gatcgtggag cacgaggtcg agtacgagtc gatcctggtg
     1741 gccttcgagg acgccgagta ctcgaccgag gcggtcgcga tggcggtgcg cctggccgca
     1801 cggcgccggc gcggcatcca cgtgctggtg accatcaccg tgcccgccaa cgccccgatc
     1861 gacgccagca tgcccgacgc cgaggccaag gcgcaggcgg cggtggaccg cgcgcgggtg
     1921 cggggcgggc gccgggtcac cggccactac gagaaggtcc gcgccggcgg cgccgggcgc
     1981 cgcatcgtca gcgaggcgcg cgagatccac gccgcagcca tcgtgatgcc gctgccgcgc
     2041 aagcgcgcgg gcagctcgct cttcgggcgg acgctcgaga ccgtgctggc cgagcggccg
     2101 tgccgggtga tcatcgactc ctccctgggg atggccaagt ccggcgacaa ggcggcggcc
     2161 tgatggagcg ctttctcggg gcgtcgagcc tggtcttggg cacgctcctg gccgtgctcg
     2221 gcgtggtcat ggtggtcagc accctggcgc gcgggggcgg cccgctggcc ttcggcgtga
     2281 tcctcggcgt gctgttcgtg ctggccggcg ccgggcggct gtacctggcc ggcgtgcggc
     2341 cgcggcgagg cgggtcgcgg tgaacgccga ggggcgcgtg gcgcgcggcc tgggccagcc
     2401 ggccctgttc gccatcggga tcagcgcggt ggcgtcctcg ctgtactacg tgctgggcat
     2461 cgtggcgggg gacgcccagg ggctgacgcc gctggtgttc gccgtcggcg cgctgttctt
     2521 cgtcctgacc gtgctcacct acgtggaggg cagctcgctg catcccgagc gcggcggcgc
     2581 gtccgtgctg gcccggtacg cgttcaacga gctgtggagc ttcatcgccg gctgggcgat
     2641 cctcctggac ttcctgatcg tgctggccat cggtgccgtg gcggtcccgc actacctgtc
     2701 cgccttctgg gggcccgcgg acgagg
//
LOCUS       DASUPW010000279         3429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1450329, whole genome shotgun sequence.
ACCESSION   DASUPW010000279 DASUPW010000000
VERSION     DASUPW010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450329"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2790)
                     /locus_tag="VFY44_06355"
     CDS             complement(<1..2790)
                     /locus_tag="VFY44_06355"
                     /inference="COORDINATES: protein motif:HMM:NF009588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX5910097.1"
                     /translation="MAAPPKAPVALSDRYTAEEGTVVLSGIQALVRMMLEQRRLDASR
                     GLDTGMYVSGYQGSPLGGLDKELARAAGHTGPAGIVFQPGLNEELAATAVAGTQLLGE
                     LEGRRHDGVTGFWYGKSPGLDRAADAIRHGNLSGTAPLGGAVALIGDDPAAKSSTVPS
                     SCEPMCRSLMLPVLAPGSVAELVSLGLHAVALSRHSGLWAGLKIVADIADASATVDAA
                     AARAGLPELPPRSGFEPPVLLPPSNLAAEHDLMGRRLELARQYGRVAGLNRVVSEPAR
                     PRTAVVAAGVAHQAVLRALDDLGLGPDACDALGLRVVQLGMPWPLDTGAVRELTAGVE
                     TVLVVEDKLPFVEGLLKEALYRTPDAPLVVGELDGEGRPLVPTRATVGADDVARALGR
                     VLDRDALPESATDRLARLEKRGDRTVPAGAALPKRTPYFCSGCPHNTSTRAQPDELVG
                     VGIGCHTMVALDQGGRRGQLVGMPQMGGEGAQWIGLAPFTDDPHFIQNLGDGTFHHSG
                     SLAIRASVAAGVSVTYRLLYNSAVAMTGGQDPEGKLDVPAITRLLELEGVARVVVTTP
                     EPERYRGVSLAASATVRHRDELQEVQAELAAVPGVTVLIHDDQCATEKRRLRKRGKLA
                     QPAEKVWINQRVCEGCGDCGEKSSCLSVQPVATEFGRKTQIHQASCNSDLTCLKGDCP
                     SFVMVTPGEGKAAHVTPEPPADLPDPRPLVPDDMLVRMPGVGGTGVVTVSAILQMAAH
                     LDGRHASGLEQTGLAQKGGPVISDVRLSSAPIQGQLRAGAASADLILGFDLLGAAASE
                     TLAVADRRRTVAVLNTAQTPTARMITDAAEAMADTDLALGRVDRATRSGDNVRVDASG
                     LAHDLFGDHMPANMLLVGAAYQQGALPIGHRAIEQAIELNGAAVDTNLAAFRWGRAVV
                     ADPEAVARAVAPEPAD"
     gene            complement(2796..>3429)
                     /locus_tag="VFY44_06360"
     CDS             complement(2796..>3429)
                     /locus_tag="VFY44_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910098.1"
                     /translation="EDPSRWTATGVAAAIRAAVAHRGLDLASTRVLIQGAGHVGEVLA
                     RLLAADGATVLIADVDGDRAASLAAELDGEVVDPRTVVGTRCDVFAPCAVARVIDPDT
                     VARLHCSIVAGAANDTLSHPADAELLAARDITYVPDFVANAGGVVDIHALRMGWDEDR
                     TRAEVLRIGDRTARLLEAADEQGWTPLAVAGQMAADRLAAARPAQDARVA"
BASE COUNT          416 a         1283 c         1312 g          418 t
ORIGIN      
        1 gtcggcgggc tccggggcca cggcccgggc caccgcctcg ggatcggcca cgaccgcccg
       61 gccccagcgg aacgcggcca ggttggtgtc cacggcggcg ccgttgagct cgatcgcctg
      121 ctcgatcgcc cggtggccga tgggcagggc gccctgctgg taggcggcgc cgaccagcag
      181 catgttggcc ggcatgtggt cgccgaacag gtcgtgggcc aggccggacg cgtccacccg
      241 cacgttgtcg cccgagcggg tcgcgcggtc cacgcgcccc agcgccaggt cggtgtcggc
      301 catggcctcg gccgcgtccg tgatcatccg ggcggtcggc gtctgggccg tgttcagcac
      361 ggccacggta cgcctgcggt cggccacggc cagcgtctcg gaggccgcgg cgcccaggag
      421 gtcgaagccc aggatcaggt ccgccgaggc cgcaccggcg cgcagctgcc cctggatggg
      481 ggctgaggac aggcgcacgt cggagatcac gggcccgccc ttctgcgcca gcccggtctg
      541 ctccagcccg gaggcgtgcc ggccgtccag gtgagcggcc atctggagga tggccgacac
      601 cgtgaccacg ccggtgccgc ccacgccggg catgcgcacc agcatgtcgt cgggcaccag
      661 cggccgcgga tccgggaggt ccgccggggg ctcgggcgtg acgtgcgcgg ccttgccctc
      721 cccgggcgtg accatcacga acgacgggca gtcgcccttg aggcaggtca ggtcgctgtt
      781 gcacgaggcc tgatggatct gggtcttgcg cccgaactcg gtcgccaccg gctggaccga
      841 caggcacgag gacttctcgc cgcagtcgcc gcagccctcg cacacgcgct gattgatcca
      901 caccttctcg gccggctgcg ccagcttgcc gcgcttgcgc aggcgccgct tctcggtcgc
      961 gcactggtcg tcgtggatga gcaccgtgac gccggggacc gccgcgagct cggcctgcac
     1021 ctcctggagc tcgtcgcggt ggcgcaccgt cgcggaggcg gccagggaga cgccgcggta
     1081 gcgctcgggc tcgggcgtgg tgaccaccac gcgggccacg ccctcgagct cgagcagccg
     1141 ggtgatggcg ggcacgtcca gcttgccctc ggggtcctgg ccgccggtca tggcgacggc
     1201 cgagttgtag agcaggcggt aggtcacgct gaccccggcg gccacggacg ccctgatggc
     1261 cagcgagccc gagtggtgga aggtgccgtc gcccaggttc tggatgaagt gcgggtcgtc
     1321 ggtgaacggc gccaggccga tccactgcgc cccctcgccg cccatctgcg gcatgccgac
     1381 cagctgcccg cggcgcccgc cctggtccag ggcgaccatg gtgtggcagc cgatccccac
     1441 gcccaccagc tcgtccggct gcgcccgggt cgaggtgttg tgagggcagc ccgagcagaa
     1501 gtacggcgtg cgcttgggca gcgcggcgcc ggccgggacg gtgcggtccc ccctcttctc
     1561 cagccgcgcc aggcggtcgg tggcggactc cggcagcgcg tcgcggtcca ggacgcgacc
     1621 gagcgcgcgg gccacgtcgt cggcaccgac ggtggcgcgc gtgggcacga gcgggcgacc
     1681 ctcgccgtcg agctccccca ccaccagcgg agcgtcgggc gtgcggtaca gggcctcctt
     1741 gagcaggccc tccacgaacg ggagcttgtc ctcgaccacg agcacggtct ccacgccggc
     1801 ggtcagctcg cggacggcgc cggtgtccag cggccagggc atgcccagct gcacgacgcg
     1861 caggccgagg gcgtcgcagg cgtccgggcc caggcccagg tcgtccagcg cgcgcaggac
     1921 cgcctggtgg gccaccccgg cggccacgac ggcggtgcgc gggcgcgcgg gctcggagac
     1981 cacgcggttc agcccggcca cgcgcccgta ctggcgcgcc agctccagcc gccggcccat
     2041 caggtcgtgc tcggcggcca ggttcgaggg cggcagcagc accggcggct cgaaccccga
     2101 ccgtggcggc agctcgggca gcccggcgcg cgcggcggcg gcgtcgacgg tggcgctggc
     2161 gtcggcgatg tcggccacga tcttcagccc cgcccacagg ccgctgtggc gcgacagggc
     2221 cacggcgtgc aggcccaggg agaccagctc ggcgaccgag ccgggagcca ggacgggcag
     2281 catcaggctg cggcacatgg gctcgcacga gctgggcacc gtcgaggact tcgcggccgg
     2341 gtcgtcgccg atcagcgcca ccgcgccacc cagcggggcg gtgccggaca ggttgccgtg
     2401 gcggatggcg tcggcggcac ggtccaggcc cgggctcttg ccgtaccaga agccggtgac
     2461 cccgtcgtgg cggcggccct ccagctcgcc caggagctgg gtgccggcca cggcggtggc
     2521 ggccagctcc tcgttcagcc ccggctggaa cacgatgccc gccgggccgg tgtgcccggc
     2581 ggcgcgggcc aattccttgt ccaggccgcc cagcggcgag ccctggtagc cgctgacgta
     2641 catgccggtg tccagcccgc gggaggcgtc caggcggcgc tgctcgagca tcatgcgcac
     2701 cagcgcctgg atgccggaga gcacgaccgt gccctcctcg gcggtgtagc ggtccgagag
     2761 ggccaccggc gccttcgggg gcgcagccac cttcatcacg ccacccgcgc gtcctgggcg
     2821 gggcgggcgg cggccaatcg gtccgccgcc atctggccgg ccaccgccag cggcgtccag
     2881 ccctgctcgt cggctgcctc cagcaggcgg gcggtgcggt ccccgatacg cagcacctcg
     2941 gcgcgcgtgc ggtcctcgtc ccaccccatc cgcagtgcgt ggatgtccac caccccgccg
     3001 gcgttggcca cgaagtcggg cacgtaggtg atgtcacggg cggccagcag ctcggcgtcg
     3061 gcgggatggc tgagcgtgtc gttggcggct ccggcgacga tcgagcagtg cagccgggcc
     3121 acggtgtcgg ggtcgatgac ccgggcaacg gcgcagggcg cgaacacgtc gcagcgggtg
     3181 ccgaccacgg tgcgcgggtc gacgacctcc ccgtcgagct cggcggccag ggacgcggcg
     3241 cggtcgccgt ccacgtcggc gatcagcacg gtggcgccgt cggcggccag cagccgggcc
     3301 agcacctcgc ccacgtgccc ggcgccctgg atcagcacgc gcgtggacgc caggtccagg
     3361 ccgcggtgcg cgaccgcggc gcggatcgcg gcggccaccc cggtcgcggt ccagcgcgac
     3421 gggtcctcg
//
LOCUS       DASUPW010000280         3194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1450407, whole genome shotgun sequence.
ACCESSION   DASUPW010000280 DASUPW010000000
VERSION     DASUPW010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450407"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VFY44_06365"
     CDS             complement(<1..488)
                     /locus_tag="VFY44_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910099.1"
                     /translation="MCVALLALFVAMGGSAYAAKKIRAKDIGTGAVGTRAVKNGSLAG
                     TDIRGNSLGPGTIAEQSLDTKKLKTVPTARVAGTVVKNAVGASELANVTRRFGGAVTI
                     VNGQAAKADVGCAKGEIALGGGGRWDNTGPGLSLQSSYADTDTGWTAIGFNASGGSRK
                     LQA"
     gene            complement(561..953)
                     /locus_tag="VFY44_06370"
     CDS             complement(561..953)
                     /locus_tag="VFY44_06370"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HEX5910100.1"
                     /translation="MERTPVFYDRDCGVCRTILAGLLAWDRGGRLRPVEIQSPEGQAL
                     LGDMTEQERLGSWHIAADGGIRSAGAAFPDLFDLLPGGGPLAALSRHAPGPSERAYRL
                     VAGNRSKLSKLVPAQARDRADRLIARRM"
     gene            966..1574
                     /locus_tag="VFY44_06375"
     CDS             966..1574
                     /locus_tag="VFY44_06375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX5910101.1"
                     /translation="MPGSATLTHVDRAQAKLWLQKWRWYERNALPWNRLAIHREMAAR
                     GAFARWPIQGNVLEMLREGRLQVGEGTLFEPQVWLTAPDDARIEIGQGTFLNIGCMVA
                     AVDLVSIGDHCMFANGCFVTDGAHRFDDPDKPVTWQGFTSKGPTRIGDNVWCGANVVV
                     TSGVTIGERCVIGANSVVTQDLPPRSIAAGVPARVLREIEYG"
     gene            complement(1581..2087)
                     /locus_tag="VFY44_06380"
     CDS             complement(1581..2087)
                     /locus_tag="VFY44_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910102.1"
                     /translation="MKNLNRLVPSPALVIACIALFVAMGGSAYALATGSISGREIRAY
                     TITGKDVKKDGLGGVSIKESRLGTVPSASGLQRSVVVGPLGQFVRGKDVASTGRTANG
                     RYLVTFRSNIQGCAYVASLGDPSVAAPPSGQVSTSGVATNPNQVQVRTTAANGDNSNR
                     PFHLIVSC"
     gene            complement(2161..3051)
                     /gene="panB"
                     /locus_tag="VFY44_06385"
     CDS             complement(2161..3051)
                     /gene="panB"
                     /locus_tag="VFY44_06385"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX5910103.1"
                     /translation="MSARPKAEAPAAKGKPVTLSALAQKKALGEPIAMVTAYDFPSAQ
                     VAEEAGVDVVLVGDSGAMTVLGYPSTVPVTTDEMLMLAAAVRRGLRTPMLVGDLPFGS
                     YEVSDEQAVATAQRFVKEAGCDAVKLERGGSSVQRAKAIIDAGIPVMGHVGLTPQTAT
                     ALGGYRSQGRTAERALEVAEDALALQEAGCFSIVFEAIPSDLTAELMPRLEIPVIGIG
                     AGPATDGQVLVFHDLLGIYDGHAARFVKRYGDVRKAMIDGVSAYAEDVRERRYPEAEH
                     GYTMAPDERERLRELLAQRV"
BASE COUNT          429 a         1119 c         1179 g          467 t
ORIGIN      
        1 gcctgcagct tgcgcgaccc gccgctggcg ttgaacccga tcgcggtcca cccggtgtcg
       61 gtgtccgcgt agctggactg gagcgaaagg cccgggccgg tgttgtccca gcgaccaccg
      121 ccgccgagcg cgatctcgcc cttggcgcag cccacgtcgg ccttggcggc ctggccgttg
      181 acgatcgtca ccgcgccgcc gaagcggcgg gtcacgttgg ccagctcgga ggcgccgacc
      241 gcgttcttga ccacggtgcc cgccacgcgg gcggtcggca cggtcttgag cttcttggtg
      301 tccaggctct gctcggcgat ggtgccgggg cccaggctgt tgccgcggat gtcggtgccg
      361 gccagggagc cgttcttcac ggcccgggtg cccacggctc cggtgccgat gtccttcgcc
      421 ctgatcttct tggcggcgta ggcggatccg cccatggcca cgaacagggc gagcagggcg
      481 acgcacatcg cgggggacgg gagggagagc tgtagtcggc gcatacccgg cccatgcccg
      541 ggccgggcac gtggcaaccg ctacatgcgg cgggcgatca ggcggtcggc ccggtcgcgt
      601 gcctgcgcgg gaacgagctt cgacagcttg gagcggttgc ccgccaccag ccggtaggcc
      661 cgctcggacg gccccggcgc gtggcggctc agcgcggcca gcgggccgcc accgggcagg
      721 aggtcgaaca ggtccgggaa cgcggcgccg gcggaccgga tcccgccgtc ggcggcgatg
      781 tgccacgagc ccagccgctc ctgctcggtc atgtcgccca ggagggcctg gccctcgggc
      841 gactggatct cgaccggtcg gagccgcccg ccgcggtccc aggccagcag gccggccagg
      901 atcgtgcggc agacaccgca gtcgcggtcg tagaagacgg gcgtgcgttc catgtccgcg
      961 agggtatgcc cggctctgcg acgctcaccc acgtggaccg ggctcaggcc aagctgtggc
     1021 tgcagaagtg gcgctggtac gagcgcaacg cgctgccctg gaaccggctg gcgatccacc
     1081 gcgagatggc cgcgcgcggc gccttcgccc gctggccgat ccagggcaac gtgctggaga
     1141 tgctgcgcga gggccggctc caggtggggg agggcaccct cttcgagccc caggtgtggc
     1201 tcaccgcgcc cgacgacgcg cggatcgaga tcggccaggg gacgttcctg aacatcggct
     1261 gcatggtggc cgcggtggac ctggtcagca tcggcgacca ctgcatgttc gccaacggct
     1321 gcttcgtcac cgacggggcg caccgcttcg acgacccgga caagccggtc acctggcagg
     1381 gcttcaccag caagggcccg acccggatcg gcgacaacgt gtggtgcggc gccaacgtgg
     1441 tggtcacgtc gggcgtgacc atcggcgagc gctgcgtgat cggcgccaac agcgtggtca
     1501 cgcaggacct cccgccgcga tccatcgcgg cgggagtgcc cgcgcgggta ctacgcgaga
     1561 tcgagtacgg ctaggccgcc tcagcagctc acgatcaggt ggaacggccg gttggagttg
     1621 tctccgttgg ccgcggtggt gcgcacctgg acctggttgg ggttggtcgc cacgccggag
     1681 gtggacacct ggccggacgg gggagcggcg acgctggggt cgccgagcga cgccacgtag
     1741 gcgcagccct ggatgttgct gcggaacgtg accaggtagc ggccgttggc ggtgcgcccg
     1801 gtgctggcga cgtccttgcc gcgcacgaac tggccgagcg ggccgaccac gaccgaacgc
     1861 tgcaggccgg acgccgaggg cacggtgccc agacgggact ccttgatgga gacgccgccg
     1921 aggccgtcct tcttcacgtc cttgccggtg atggtgtagg cgcggatctc gcgaccgctg
     1981 atcgagccgg tggcgagcgc gtaggcggag ccgcccatgg ccacgaacag ggcgatgcag
     2041 gcgatgacca gtgccgggga cggcaccaat cggttcaggt tcttcaaggg gacctcctgg
     2101 tgggattcgg atgggggttc tgcacagggc aacccgccac ctcccccaaa ggtgctgtgc
     2161 ctacacgcgc tgggccagga gctcccgcag ccgctcgcgc tcgtcgggcg ccatcgtgta
     2221 gccgtgctcg gcctccgggt agcgccgctc gcgcacgtcc tcggcgtagg cgctcacccc
     2281 gtcgatcatg gccttgcgca cgtcgccgta gcgcttgacg aaccgggccg cgtgcccgtc
     2341 gtagatgccg agcaggtcgt ggaagaccag cacctggccg tcggtcgcgg gacccgcgcc
     2401 gatgccgatc accgggatct caaggcgcgg catcagctcg gcggtcaggt cgctggggat
     2461 ggcctcgaac acgatcgaga agcaccccgc ctcctggagc gccagcgcgt cctcggccac
     2521 ctcgagcgcg cgctcggcgg tgcgaccctg cgagcggtag ccgcccagcg ccgtggcggt
     2581 ctggggcgtc aggcccacgt ggcccatcac ggggatgccg gcgtcgatga ttgccttcgc
     2641 ccgctgcacc gagctcccgc cgcgctccag cttgacggcg tcgcagcccg cctccttgac
     2701 gaaccgctgg gcggtggcca cggcctgctc gtcggacacc tcgtaggagc cgaacggcag
     2761 gtcgcccacc agcatcgggg tgcgcaggcc gcggcgcacg gcggcggcca gcatcagcat
     2821 ctcgtcggtg gtcaccggca ccgtgctggg gtagcccagg acggtcatgg cgccgctgtc
     2881 gcccaccagg acgacgtcca cgccggcctc ctcggccacc tgcgcgctgg ggaagtcgta
     2941 ggcggtgacc atggcgatcg gctcgcccag ggccttcttc tgggccagcg ccgacagggt
     3001 caccggcttg cccttggcgg ccggggcctc ggccttcggt cgcgcgctca tgccagcagc
     3061 tccgagaccg cgtcgtccac ggtgacgatg tcgttgtcgg cgttcacgtg gaccacgcgc
     3121 ggctcgtagt ggtccagctc ggcctcgtcg tactcggcgt agctgaccac gatgaccttg
     3181 tcgccggtgt gcac
//
LOCUS       DASUPW010000281         2140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1450441, whole genome shotgun sequence.
ACCESSION   DASUPW010000281 DASUPW010000000
VERSION     DASUPW010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450441"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..>2140
                     /locus_tag="VFY44_06390"
     CDS             20..>2140
                     /locus_tag="VFY44_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910104.1"
                     /translation="MSVLGCLGLPSAASAQSSAPTAGPNARYALANGCFALRSSAAGK
                     VVAKSGGGYAATSPQVPGAEPFTLKATALGSYMLYGPARDYLARDGAGNVAPARDSGP
                     GADWTITVQGNSFRLALGDQVLSIDGAGRLVLTGSPGPGSQFSMEKASGCAAFPEVET
                     GVTGAPSKGSTPYTETAGFLEAHMHGMAFEFLGGRAHCGRPWHRYGVEKALVDCPDHE
                     LARGGAALVENQLSYKTPARSHDPVGWPTFKDWPAAKSLTHEQSYYKWLERSWRAGQR
                     VFVNLLVENAVLCEVYPFKQNSCDEMDAVRLQARRMREMENYIDAQAGGPGKGFYRIV
                     TDPYQARRVVNEGKMAIVMGIEVSEPFGCRVYNDAPQCDRAGIDRQLDEVYRLGVRQM
                     ELINKFDNALAGVAGDTADTGVIVNNGNKLRTGKYWAMGPCDGAPDEVDRQQVGLYDH
                     DHNDLLSNAIESLLPLGVAPVYPRNSNCNARGLTELGEHVIRRMMAKRMIVDPDHLSV
                     RARKSVMSLLEAKRYSGVVSSHSWSTPDVVPRIYNLGGVITPYAGDSKSFVKKYREEA
                     PKANRKKFYFGFGFGADMNGFGAQGNPRNGPNPVTYPFKSWDGKQTIDRQKSGTRVFD
                     INKDGVAHYGLYPDWIEDLRKQAGDGIVRDLARGSEAYLQMWERAEGVRNQACRSARG
                     QFTAKGMGRQRLGAGPASVLQKAGQ"
BASE COUNT          340 a          758 c          760 g          282 t
ORIGIN      
        1 gcgcctggcc gtagtgctcg tgtccgtgct cggatgcctc ggcctgccgt cggccgcctc
       61 ggcccagtcc tcggcaccga ccgcggggcc caacgcccgc tacgccctgg ccaacggctg
      121 cttcgcgctg cgctccagcg ccgccggcaa ggtggtggcc aagtccggcg gcggctacgc
      181 ggccacctcg ccgcaggtcc ccggggccga gccgttcacg ctcaaggcca ccgcgctggg
      241 ctcctacatg ctctacgggc cggcgcgcga ctacctggcc cgtgacggcg ccggcaacgt
      301 ggcgcctgcg cgcgactccg gccccggcgc cgactggacg atcacggtgc agggcaactc
      361 gttccggctg gccctgggcg accaggtgct gtcgatcgac ggcgccggcc ggctcgtgct
      421 cacggggtcg cccgggccgg gatcgcagtt cagcatggag aaggcctcgg gatgcgcggc
      481 cttccccgag gtcgagaccg gcgtgaccgg cgccccctcg aagggctcga cgccgtacac
      541 cgagaccgcc ggcttcctcg aggcgcacat gcacggcatg gcgttcgagt tcctcggcgg
      601 ccgcgcgcac tgcggccggc cgtggcaccg ctacggggtc gagaaggccc tggtcgactg
      661 ccccgaccac gagctcgccc gcggcggcgc ggccctggtc gagaaccagc tctcgtacaa
      721 gaccccggcg cgctcgcacg acccggtcgg ctggccgacg ttcaaggact ggccggcggc
      781 caagtcgctg acgcacgagc agagctacta caagtggctc gagcgctcgt ggcgggcggg
      841 ccagcgggtg ttcgtgaacc tgctggtcga gaacgcggtg ctgtgcgagg tctatccctt
      901 caagcagaac tcgtgtgacg agatggacgc cgtccgcctc caggcgcggc gcatgcgcga
      961 gatggagaac tacatcgacg cgcaggccgg tggtccgggc aagggcttct accggatcgt
     1021 caccgacccc taccaggccc ggcgcgtggt caacgagggc aagatggcga tcgtcatggg
     1081 catcgaggtg tccgagccct tcggctgccg cgtctacaac gacgcgccgc agtgcgaccg
     1141 cgccggcatc gaccgccagc tggacgaggt gtaccggctc ggcgtgcgcc agatggagct
     1201 gatcaacaag ttcgacaacg ccctggccgg ggtggccggg gacaccgccg acaccggcgt
     1261 gatcgtcaac aacggcaaca agctgcgcac cggcaagtac tgggcgatgg gcccgtgcga
     1321 cggcgcgccc gacgaggtcg accgccagca ggtcggcctg tacgaccacg accacaacga
     1381 cctgctctcc aacgcgatcg agtcgctgct gccgctgggg gtcgcgcccg tctacccgcg
     1441 caactcgaac tgcaacgcgc gcggcctgac cgagctgggc gagcacgtga tccggcggat
     1501 gatggccaag cgcatgatcg tcgaccccga ccacttgagc gtccgggcgc gcaagtcggt
     1561 gatgtcgctg ctggaggcca agcgctactc cggcgtggtg tccagccaca gctggagcac
     1621 cccggacgtg gtgccgcgga tctacaacct cggcggcgtg atcaccccgt acgccggcga
     1681 ctcgaagtcg ttcgtgaaga agtaccgcga ggaggccccc aaggccaacc gcaagaagtt
     1741 ctacttcggc ttcggcttcg gcgcggacat gaacggcttc ggggcgcagg gcaacccgcg
     1801 caacggcccc aacccggtca cgtacccgtt caagtcctgg gacggcaagc agaccatcga
     1861 caggcagaag tcgggcacgc gcgtgttcga catcaacaag gacggcgtgg cccactacgg
     1921 cctctacccg gactggatcg aggacctgcg caagcaggcg ggcgacggaa tcgtgcggga
     1981 cctggcccgc gggtccgagg cctacctgca gatgtgggag cgcgccgagg gcgtgcgcaa
     2041 ccaggcctgc cgctcggcgc ggggccagtt caccgcgaag gggatggggc gccagcgcct
     2101 gggcgccggg ccggcatcgg tgctgcagaa ggcgggccag
//
LOCUS       DASUPW010000282         2746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_2005345, whole genome shotgun sequence.
ACCESSION   DASUPW010000282 DASUPW010000000
VERSION     DASUPW010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2005345"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1531
                     /locus_tag="VFY44_06395"
     CDS             <1..1531
                     /locus_tag="VFY44_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5910105.1"
                     /translation="ELGACEIHVSVPDALDRLREAHATLEDPAMRAVAAEGLTRALVF
                     AGDPDEVVEVADRTIAEVPEGMDDVRDTLEALALVARAFGAADTGMEERSERARAKGA
                     GTSHGAHMRAAVAAWDWTCHGGTAAECAELALAALADGSLVANDPGFFTPVAAATLIN
                     ADHPELPALWERTQAEAHRRGSLFAMVGVNLWRGLTLLHRGELEEAEASLLSGFDRND
                     VFGTKSMSAGAYTVGFLARVYLEQGKVREARVMCDAAGPEGPGARAVPGSDGDLYRRR
                     GVIELLLAEDRAEEALAEIEAMVALLRRTTNPSVVPWRTMKALALDRLGRADEAVAAA
                     AEELEPARHWGAPSCLGRTLRVLGTLEREQGIAHLEEAVAVTEGTPARLEHAKALAAL
                     GSAVRRDRRPSDSREPLRRALELAESCGATPLMEFARSELLASGARPRTSALGGVESL
                     TPSERRVADLALGGQTNRDIAQTLFVTPKTVEVHLSNTYRKLGISSRRELAGAMTPAE
                     A"
     gene            1568..2491
                     /locus_tag="VFY44_06400"
     CDS             1568..2491
                     /locus_tag="VFY44_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5910106.1"
                     /translation="MLLVLAVGGCGGDDQKAASQAPPATLALDFQPNAVHAGIFTAVD
                     RGLDEREDVELTVRTPAASTDSLKLLAAGKADFSVVDIHDLGLARERGQDVVGVGAIV
                     QRPLAAVIARAGVRRPRDLEGRRVGVTGLPSDQAVLESVVRADGGDPGKVRTTTIGFA
                     AVPSLAAGKVDAVVTFWNAEGVALRRRGVRTREFRVDRYGAPRYPELVLATKRSTLES
                     DPALVRRVVAALEAGTRAAAADLPAASAAVARRSQGDRALTDAQLAAVAPAFAPALKL
                     SRPRLEGWAAFDTRFGILERRPDVDRAFALD"
     gene            complement(2492..>2746)
                     /locus_tag="VFY44_06405"
     CDS             complement(2492..>2746)
                     /locus_tag="VFY44_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910107.1"
                     /translation="RRGRRGCVRGPVRVTVTGADRGLIKRANLRVGKKTLDDAKAPLS
                     KRFSTRALRRSHVRSAKASLRLRDGSLVRLSKRFRVCARR"
BASE COUNT          325 a         1023 c         1078 g          320 t
ORIGIN      
        1 ggagctgggc gcgtgcgaga tccatgtgag cgtgccggac gccctggacc gcctgcgcga
       61 ggcccacgcc accctggagg accccgccat gcgcgcggtg gcggccgagg ggctgacgcg
      121 cgcgctggtg ttcgcgggcg acccggacga ggtggttgag gtggccgacc gcaccatcgc
      181 cgaggtcccc gagggcatgg acgacgtgcg cgacacgctg gaggcgctgg cgctggtcgc
      241 gcgcgccttc ggggccgccg acaccggcat ggaggagcgc tccgagcgcg cccgagccaa
      301 gggcgcgggc accagccacg gcgcccacat gcgcgccgcg gtggcggcct gggactggac
      361 ctgccacggc ggcaccgccg ccgagtgcgc cgagctggcg ctggccgcgc tggcggacgg
      421 gtccctggtg gccaacgacc cgggcttctt caccccggtg gcggcggcca ccctgatcaa
      481 cgccgaccac cccgagctgc ccgccctgtg ggagcgcacc caggccgagg cgcaccggcg
      541 cggatcgctg ttcgccatgg tcggggtgaa cctgtggcgc ggcctgaccc tgctgcaccg
      601 cggtgagctc gaagaggccg aggcctcgct gctgagcggc ttcgaccgca acgacgtgtt
      661 cggcacgaag tccatgagcg ccggcgccta caccgtcggg ttcctggccc gcgtctacct
      721 cgaacagggc aaggtgcgcg aggcgcgggt gatgtgcgac gccgccggcc cggaagggcc
      781 cggggcgcgc gcggtgcccg gctcggacgg cgacctgtac cggcggcgcg gggtgatcga
      841 gctgctgctg gccgaggacc gcgcggagga ggcgctggcg gagatcgagg ccatggtcgc
      901 cctgctgcgc cggaccacga acccgtccgt ggtgccctgg cgcaccatga aggcgctggc
      961 gctggaccgc ctgggccgtg ccgacgaggc cgtggccgcc gccgcggagg agctcgagcc
     1021 cgcccgccac tggggcgcgc cctcctgcct gggccgcacg ctgcgcgtgc tgggcacgct
     1081 ggagcgtgag cagggcatcg cgcacctgga ggaggccgtg gccgtgaccg agggcacgcc
     1141 ggcgcgcctg gagcatgcca aggccctggc cgcgctgggc tcggccgtgc gccgcgaccg
     1201 ccggccctcg gattcgcgcg agccgctgcg ccgcgcgctg gagctggccg agtcgtgcgg
     1261 cgcgaccccg ctgatggagt tcgcgcgcag cgagctgctg gcctcgggag cgcggccccg
     1321 gaccagcgcg ctgggcggcg tggagtcgct gacccccagc gagcgccggg tggccgacct
     1381 ggcgctgggc gggcagacca accgcgacat cgcccagacg ctgttcgtga ccccgaagac
     1441 ggtcgaggtc cacctgagca acacctaccg gaagctgggc atcagctcac ggcgcgagct
     1501 ggccggggcg atgacgcccg ccgaggcctg agccgtgccg gcgaagggcc cggctgcgct
     1561 gctcgtcgtg ctgctcgtgc tcgccgtcgg cgggtgcggc ggcgacgacc agaaggccgc
     1621 cagccaggca ccgccggcca ccctggccct ggacttccag cccaacgccg tgcacgccgg
     1681 gatcttcacc gccgtggaca ggggcctgga cgagcgcgag gacgtggagc tgaccgtgcg
     1741 cacccccgcc gcctcgaccg actcgctgaa gctcctggcc gccggcaagg ccgacttctc
     1801 cgtggtcgac atccacgacc tgggcctggc ccgcgagcgc ggccaggacg tggtgggcgt
     1861 gggcgccatc gtccagcggc cgctggcagc cgtgatcgcc cgcgcgggcg tccgccggcc
     1921 gcgcgatctg gagggccggc gcgtgggcgt gaccgggctg ccctcggacc aggcggtgct
     1981 ggagtccgtg gtccgcgccg acggtggcga cccggggaag gtgcgcacca ccaccatcgg
     2041 cttcgcggcc gtgcccagcc tggccgcggg caaggtggac gcggtggtca cgttctggaa
     2101 cgccgagggc gtcgcgctga ggcggcgggg cgtgcgcacg cgcgagttcc gcgtggaccg
     2161 ctacggcgcg ccccgctacc ccgagctggt gctggccacc aagcggtcga cgctcgagtc
     2221 cgaccccgcg ctcgtgcggc gcgtcgtcgc cgcgctcgag gccggcaccc gtgccgcggc
     2281 ggcggacctc ccggccgcat cggccgcggt ggccaggcgc tcccagggcg accgggcgct
     2341 cacggacgca cagctggccg cggtggcgcc cgcgttcgcg cccgcgctga agctgagccg
     2401 cccccggctg gagggctggg cggcgttcga cacccgcttc gggatcctcg agcgccggcc
     2461 cgacgtggac cgcgcgttcg cgctggactg atcagcgccg ggcacagacg cggaagcgct
     2521 tgctcagccg cacaaggctg ccgtcgcgca ggcgtagcga ggccttggcg ctgcgcacgt
     2581 ggctgcgccg cagggcgcgc gtcgagaagc gcttgctcag cggcgccttc gcgtcgtcga
     2641 gcgtcttctt gcccacgcgc aggttcgccc gcttgatcag gccgcggtcg gcgccggtga
     2701 ccgtcacccg caccgggcca cggacgcagc cgcggcgccc gcggcg
//
LOCUS       DASUPW010000283         3665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_758201, whole genome shotgun sequence.
ACCESSION   DASUPW010000283 DASUPW010000000
VERSION     DASUPW010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758201"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VFY44_06410"
     CDS             complement(<1..202)
                     /locus_tag="VFY44_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910108.1"
                     /translation="MNRLIALGTLVLALSIPAAARADVTVLSAEGDELVSFKSAACKK
                     GGKKLLSFTGLAKSGGWKLRVNI"
     gene            complement(286..1257)
                     /locus_tag="VFY44_06415"
     CDS             complement(286..1257)
                     /locus_tag="VFY44_06415"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX5910109.1"
                     /translation="MSFTPPPIPADEARRLQSLRDLGILDTEAEERFDRITRLVQRIL
                     DVPMSAITLVDADRQWFKSRQGIDDPETSREHSFCAHAVAQEGLLYVPDAHEDDRFAG
                     NPLVLDDPNVRAYAGAQIHAPDGAAIGTLCAIDDRPRELTAEDLQALEDLARMAEQEI
                     AALNMAITDGLTGLANRRGLMTLGGKVLSAAGRMGFPVEMLFMDVDGLKRVNDELGHD
                     RGDELLRDVAGLLEATFRESDVIARLGGDEFCVLLTGTATAGAEGAVVRLQAAIDQHN
                     DAAGRPYAVAVSVGHAGLEGEESPDLEDLLRRSDAAMYDHKRSRRRR"
     gene            complement(1304..2251)
                     /locus_tag="VFY44_06420"
     CDS             complement(1304..2251)
                     /locus_tag="VFY44_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5910110.1"
                     /translation="MSFASPLFLLALLALPAAIGLQHLMRSRQRRHAIRFTGVSTLAG
                     VVPNASRVRRHVPLALFLAALAVLVLALAKPERSVGVPVERASVTLVTDVSRSMKADD
                     VKPNRLEAARKAGERFLDQAPPQLRIGSVTFSDTPQIVESPSDDRNEARSVLDGLVAD
                     GGTATGDGLQAALDSLDRERGKDGKREPAAILLLSDGESTIGRDPVGVAREAGQRGVP
                     VFTVALGTESAVVEGPNGELLPVPPDPETLRRMSEVSGGQAFTATESDQLDNVYEKLG
                     SKIGQKQEKREMTAGFAGAGAVLLLLAAGAAMRLGGRLP"
     gene            complement(2248..3132)
                     /locus_tag="VFY44_06425"
     CDS             complement(2248..3132)
                     /locus_tag="VFY44_06425"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5910111.1"
                     /translation="MPGAVLGPLELEMARRTSSPLSGDRPAPGVGQGTEIAQLRPYQP
                     GDDVRRLDPAATARTGQPHVRLQVPERQLTTWMVLDMSASMAFGTADRLKSDVAEGAA
                     LTVGRIATRRGGRLGIVTSGTEHERRLPPRGGRTGYVALTRVLEEGVGPDGSGHDDAL
                     TVALERTGRLASRTGLVCVISDFRGPRTWRRALSSLAHRHSILAIEVRDPREGALPDV
                     GQLSLVDPETGKHLRVDSSDPRLRRRYEASERRQREQVAADLRRAGARHVVLRTDRPW
                     LRDLGRELSAGAHHGVRR"
     gene            complement(3195..>3665)
                     /locus_tag="VFY44_06430"
     CDS             complement(3195..>3665)
                     /locus_tag="VFY44_06430"
                     /inference="COORDINATES: protein motif:HMM:NF036831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5910112.1"
                     /translation="TEEATVVERVIGVPAQVEQRLTMEKLLDYREAVRAIYVDREVVD
                     HAVELADATRRPDRFGLDDLLHLVDYGASPRGPISLVQSAQALALLRGRRYVLASDVR
                     DLAPDVLRHRLVLSYDALSDGVDADDILDRVLDAIPEPGAEAEPPEEYREASAA"
BASE COUNT          460 a         1352 c         1372 g          481 t
ORIGIN      
        1 agatgttgac ccgcagcttc cagccgccgc tcttggcgag ccccgtgaac gacaggagct
       61 tcttcccgcc cttcttgcac gccgcgctct tgaaggacac caactcgtcc ccctccgcgc
      121 tgaggaccgt gacgtcggcc cgggccgccg ccgggatcga gagtgcgagg accagggtgc
      181 cgagcgcgat caagcggttc atggcggcca gcctacgggc gttcgcgggc cctcgcctca
      241 ggaccacccc cgaggttttg ccggctcctc ccccgagatt ttcggctagc gccggcgccg
      301 cgagcgcttg tggtcgtaca tggccgcgtc gctgcggcgg agcaggtcct cgaggtcggg
      361 ggactcctcc ccctcgagcc cggcgtggcc cacgctgacc gcgacggcgt agggtcggcc
      421 ggccgcgtcg ttgtgctggt cgatcgcggc ctggaggcgc accaccgccc cctcggcacc
      481 ggccgtggcc gttcccgtga gcagcacgca gaactcgtcg ccgcccaggc gcgcgatgac
      541 gtcggactca cggaaggtgg cctccaggag gcccgcgaca tcacgcagga gctcgtcgcc
      601 ccggtcatgg cccagctcgt cgttgacgcg cttgaggccg tcgacgtcca tgaacaacat
      661 ctccaccggg aagcccatcc ggccggcggc cgacagcacc ttgccgccca gcgtcatcag
      721 cccgcggcgg ttcgcgagcc cggtcaggcc gtcggtgatg gccatgttca gggcggcgat
      781 ctcctgctcg gccatgcgcg ccaggtcctc cagcgcctgt aggtcctcag cggtgagctc
      841 gcgcgggcgg tcgtcgatgg cgcacagggt gccgatggcc gcgccgtcgg gggcgtggat
      901 ctgggcgccc gcgtaggcgc gcacgttggg gtcgtccagc accagcggat tgccggcgaa
      961 gcggtcgtcc tcgtgcgcgt cgggcacgta cagcagcccc tcctgggcca cggcgtgggc
     1021 gcagaaggag tgctcgcgcg aggtctcggg gtcgtcgatc ccctggcgcg acttgaacca
     1081 ctgccggtcg gcgtccacca gcgtgatcgc cgacatcggg acgtccagga tccgctgcac
     1141 gaggcgggtg atgcggtcga agcgctcctc ggcctcggtg tccaggatgc cgaggtcgcg
     1201 cagcgactgc aggcgccgcg cctcgtcggc ggggatcggc gggggtgtga agctcaagtt
     1261 gcgcggctcc tacccattcg gggagggaac gtatcgggat gggtcagggg agacgaccgc
     1321 ccaggcgcat cgccgctccc gcggccagca ggaggagcac cgcacccgcg cccgcgaagc
     1381 cggccgtcat ctcgcgcttc tcctgcttct ggccgatctt cgagcccagc ttctcgtaga
     1441 cgttgtccag ctggtcggac tcggtggcgg tgaacgcctg gccgcccgag acctcggaca
     1501 tgcgccgcag cgtctcgggg tccgggggca cgggcagcag ctcgccgttg gggccctcga
     1561 ccaccgcgct ctcggtgccg agcgcgacgg tgaacaccgg cacgccgcgc tggccggcct
     1621 cgcgggccac gcccacgggg tcgcggccga tcgtggactc gccgtcggag agcagcagga
     1681 tcgccgcggg ctcgcgcttg ccgtccttgc cgcgctcgcg gtccaatgag tccagcgccg
     1741 cctgcaggcc gtcgccggtg gccgtgcccc cgtcggccac caggccgtcg agcaccgagc
     1801 gcgcctcgtt gcggtcgtcg gaagggctct cgacgatctg gggggtgtcc gagaacgtga
     1861 ccgacccgat ccgcagctgc ggcggcgcct ggtccaggaa ccgctcgccc gccttgcgcg
     1921 ccgcctcgag ccggttgggc ttcacgtcgt cggccttcat cgaccgcgac acgtcggtca
     1981 ccagcgtgac cgaggcccgc tcgacgggaa ccccgacgct gcgctcgggc ttggccagcg
     2041 ccagcaccag cacggcgagc gcggccagga acagcgccag cggaacgtgc cgccggaccc
     2101 ggctggcgtt gggcacgacg cccgccaggg tggacacgcc ggtgaagcgg atggcgtggc
     2161 ggcgctggcg gctgcgcatc aggtgctgca ggccgatcgc ggccggcagc gccaggagcg
     2221 ccagcaggaa cagggggctg gcgaagctca ccgccgcacc ccgtggtggg cgccggcgga
     2281 cagctcacgg cccaggtccc gaagccacgg gcggtcggtg cgaaggacca cgtgccgggc
     2341 cccggcgcgg cgcaggtcgg ccgccacctg ctcgcgctgg cggcgctccg aggcctcgta
     2401 gcgccggcgc agccgcgggt cggagctgtc cacgcgcagg tgcttgccgg tctcggggtc
     2461 gaccagcgag agctggccga cgtcgggcag cgcgccctca cgcgggtcgc gtacctcgat
     2521 ggccaggatc gagtggcggt gggccaggga gctcagcgcc cggcgccagg tgcgagggcc
     2581 gcggaagtcg gagatcacgc acaccaggcc ggtccgcgag gccaggcggc cggtgcgctc
     2641 gagggcgacg gtgagcgcgt cgtcgtggcc ggagccatcg gggccgacgc cctcctcgag
     2701 cacgcgcgtc agcgccacgt agcccgtgcg cccgccacgc ggcggcagcc gccgctcgtg
     2761 ctcggtgccg gaggtcacga tgcccagccg gccgccgcgc cgggtggcga tccggccgac
     2821 cgtcagggcg gccccctcgg cgacgtcgga cttcagccgg tcggccgttc cgaacgccat
     2881 cgaggcggac atgtccagca ccatccacgt ggtcagctgg cgctcgggca cctgcaggcg
     2941 cacgtgcggc tggcccgtac gcgccgtcgc ggccgggtcg agccggcgca cgtcgtcacc
     3001 aggctggtag gggcgcagct gggcgatctc ggtgccctgc cccacaccgg gtgcgggacg
     3061 gtcgccggag agcggcgagc tggtgcgccg cgccatctcc agctccagcg ggcccagcac
     3121 cgcgcccggc atggggccgg ggccctggct cccgggcgga cgccgcgcgc cccggcgacg
     3181 ggggtcggcg ggcctcaagc ggccgacgcc tcccggtact cctcgggagg ctcggcctcg
     3241 gcgccgggct cggggatcgc atccagcacg cggtccagga tgtcgtcggc gtccacgccg
     3301 tcgctgagcg cgtcgtagct gagcaccagc cggtggcgca gcacgtcggg cgccaggtcg
     3361 cgcacgtcgg aggccagcac gtaccggcgg ccgcgcagca gcgccagcgc ctgggcggac
     3421 tgcaccagcg agatcggccc gcgcgggctg gcgccgtagt ccaccaggtg cagcaggtcg
     3481 tccaggccga accggtcggg gcggcgggtg gcgtcggcca gctcgacggc gtggtcgacc
     3541 acctcgcggt ccacgtagat cgctcgcacg gcctcgcggt agtccaggag cttctccatg
     3601 gtcagccgct gctccacctg ggcggggacg ccgatcacgc gctcgaccac cgtggcctcc
     3661 tcggt
//
LOCUS       DASUPW010000284         3605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1036108, whole genome shotgun sequence.
ACCESSION   DASUPW010000284 DASUPW010000000
VERSION     DASUPW010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036108"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VFY44_06435"
     CDS             complement(<1..435)
                     /locus_tag="VFY44_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910113.1"
                     /translation="MTIHLSFPLFLPLATALVAAFLPARIGRWLVVAGTVAVLGYAVA
                     LVVDFDSADAGLQYVTDDEWIAELGIRYQLGVDGLNLFLILTTALLWVPATVWAAVRE
                     TERTKLYLFHLALGETAILGAFMAQDLALFVVFFDLMLVPFYF"
     gene            complement(432..2441)
                     /gene="nuoL"
                     /locus_tag="VFY44_06440"
     CDS             complement(432..2441)
                     /gene="nuoL"
                     /locus_tag="VFY44_06440"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX5910114.1"
                     /translation="MTPTDYGWLVLLFPLLGTVLIMLGWPRWPGRSAGWIASAAIGLA
                     FASSVGMLLTLNDRPEEGKSLIGTAYEYIGTSGFNADLSILVDPLSIVMCLVVTGVSF
                     LIHLYSVSYMTSDPGFTRFFAYLNFFVFSMLVLILAANFVLLIVGWAFVGAASYLLIS
                     FWYRRTTATAAGMKAFVINVVGDIGLVIGAFLLFDKTGALDYPGVFLRAPDVFSTNDT
                     QLVIACLLLLVGAFAKSAQLPLHTWLPDAMEGPTPVSALIHAATMVTAGVYLIARCHP
                     LFELAPTAADIGAIIGCATLIFAASIALVQTDLKRVIAYSTMSQIGYMVMGVCVAAYG
                     AGIFHLMTHAFFKALLFMGAGSVIGAMAGEQSMDRMGGFKKAMPFTYATFLIGALALA
                     GFPLMSGFFSKDEILAYTLNRGGWFVVLGVVGYIAALLTAFYAFRMVFRVFHGKAVPE
                     AEELEGGHLHHGEHYNPATGEPEDTDVGFPGPEHAIAERAFPMKVAMAPLAVLAIIAG
                     ALGIPGVTDTLEHFLEPTFADSKFHDVHPSTGAEVSGLLVGGIIAVLGIGGAFFVFIK
                     NPALRTRALERFPAVHRVLYNKYYFDEAIDLVFVRPAFATGRFGRTVVESSFVQGVLV
                     GGATGAVRLGTAFARSIQTGELRAYAGLLIAGVGGTVLYFLVVST"
     gene            complement(2441..2740)
                     /gene="nuoK"
                     /locus_tag="VFY44_06445"
     CDS             complement(2441..2740)
                     /gene="nuoK"
                     /locus_tag="VFY44_06445"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX5910115.1"
                     /translation="MNVEWFVVLSALIFAIGAAGVLTRRSPLVILLCLELMLNAGNLA
                     LIAFSRMVGNGDGQVFALIVMVVAACEVVIGLGIIVAMYRRRLAIDVDSVSELRG"
     gene            complement(2740..3363)
                     /locus_tag="VFY44_06450"
     CDS             complement(2740..3363)
                     /locus_tag="VFY44_06450"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX5910116.1"
                     /translation="MFFEQLLFFIAGIAALGGAIGVVTLRNQFYSVLSLIAHLLGLAV
                     LFLLLRAEFLAAAQVVVYAGAVMVLYLFVVAYIGGADEPLRPESGVLRVLGPVFALVL
                     FLELAVAVLGSSLSALDTTGPKVGEGFGSPAQIGELLLNRFLLPFEAASFLLLVAAVG
                     AVVMARKRRGLEEPESALGAITVREPVMEGADQPGRGARGGPDGRAY"
     gene            complement(3373..>3605)
                     /locus_tag="VFY44_06455"
     CDS             complement(3373..>3605)
                     /locus_tag="VFY44_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX5910117.1"
                     /translation="TPDNQVSAGERYAAVYEINMTRCIFCGYCEVACPFDAITMGNDY
                     EMADYDRSDLIFTKEMLLADHPERTPLRREGE"
BASE COUNT          668 a         1243 c         1264 g          430 t
ORIGIN      
        1 gaagtagaac ggcaccagca tcaggtcgaa gaacacgacg aacagcgcca ggtcctgcgc
       61 catgaacgcg cccaggatgg cggtctcgcc cagcgccagg tggaacaggt agagcttggt
      121 gcgctcggtc tcgcgcaccg cggcccagac cgtggccggc acccacaaca gcgcggtggt
      181 caggatcagg aacaggttca gcccgtccac gcccagctgg tagcggatgc ccagctcggc
      241 gatccactcg tcgtcggtca cgtactgcag cccggcgtcg gcggagtcga agtcgacgac
      301 cagcgcgacc gcgtagccca gcacggcgac ggttccggcg accaccagcc agcggccgat
      361 ccgggccggc aggaacgcgg cgaccagcgc cgtggccagc ggcaggaaca gcgggaagct
      421 caggtggatc gtcacgtgga caccaccagg aagtagagga cggtgccgcc cacgcccgcg
      481 atcagcaggc cggcgtacgc gcgcagctca ccggtctgga tcgagcgcgc gaaggcggtg
      541 cccaggcgca cggcgccggt ggccccgccc accaggacgc cctggacgaa gctgctctcg
      601 accacggtgc ggccgaagcg gccggtggcg aacgcgggcc gcacgaacac gaggtcgatc
      661 gcttcgtcga agtagtactt gttgtagagc accctgtgca cggccgggaa gcgctccagc
      721 gcgcgcgtgc gcagcgcggg gttcttgatg aacacgaaga acgcgccgcc gatgcccagc
      781 acggcgatga tgccgcccac cagcaggccc gacacctcgg cgccggtcga cgggtgcacg
      841 tcgtggaact tcgagtcggc gaacgtgggc tcgaggaagt gctccagcgt gtcggtcacg
      901 ccggggatgc ccagcgcgcc ggcgatgatc gccagcacgg ccagcggcgc catcgcgacc
      961 ttcatgggga acgcccgctc ggcgatcgcg tgctcggggc cggggaagcc gacgtcggtg
     1021 tcctcgggct cgccggtggc cgggttgtag tgctcgccgt ggtgcaggtg gccgccctcc
     1081 agctcctcgg cctcgggcac ggccttgccg tggaagacgc ggaagaccat gcggaacgcg
     1141 tagaacgcgg tgagcagcgc ggcgatgtag cccaccacgc ccaggaccac gaaccagccg
     1201 ccgcggttga gcgtgtaggc caggatctcg tccttcgaga agaagcccga catcagcggg
     1261 aagccggcca gggccagcgc cccgatcagg aacgtggcgt aggtgaacgg catcgccttc
     1321 ttgaagccgc ccatccggtc catcgactgc tcgccggcca tggcgccgat gaccgagccc
     1381 gcgcccatga acagcagcgc cttgaagaac gcgtgcgtca tcaggtggaa gatccccgcg
     1441 ccgtacgcgg ccacgcacac gcccatgacc atgtagccga tctgggacat cgtcgagtac
     1501 gcgatcacgc gcttgaggtc ggtctggacc agcgcgatcg aggcggcgaa gatcagcgtg
     1561 gcgcagccga tgatggcgcc gatgtccgcg gcggtcggag ccagctcgaa gagggggtgg
     1621 cagcgcgcga tcaggtacac gccggcggtg accatggtgg ccgcgtggat cagcgccgag
     1681 acgggggtgg ggccctccat ggcgtccggc agccaggtgt gcagcggcag ctgggccgac
     1741 ttcgcgaacg ctcccaccag cagcagcagg caggcgatca ccagctgcgt gtcgttggtc
     1801 gagaacacgt cgggggcgcg caggaacacg cccgggtagt ccagggcgcc ggtcttgtcg
     1861 aacagcagga acgcgccgat gaccaggccg atgtcgccga ccacgttgat cacgaacgcc
     1921 ttcatgccgg cggcggtggc cgtggtgcgc cggtaccaga agctgatcag caggtaggag
     1981 gcggcgccga cgaaggccca gcccacgatc agcagcacga agttggcggc caggatcagc
     2041 accagcatcg agaacacgaa gaagttcagg tacgcgaaga agcgcgtgaa gccggggtcc
     2101 gacgtcatgt aggacaccga gtagaggtgg atcaggaacg acacgccggt gaccaccagg
     2161 cacatcacga tcgagagcgg gtcgaccagg atcgacaggt cggcgttgaa gccggacgtg
     2221 ccgatgtact cgtacgcggt gccgatcagc gacttgccct cctcgggccg gtcgttcagc
     2281 gtcagcagca tcccgaccga cgacgcgaag gccagcccga tcgcggcgct ggcgatccag
     2341 ccggcgctgc gccccggcca gcgcggccac ccgagcatga tcaggaccgt ccccaggagc
     2401 gggaacagca ggaccagcca cccgtagtcg gtcggcgtca tcagccccgc agctccgaga
     2461 cggagtccac gtcgatcgcc agccggcggc ggtacatcgc gacgatgatc cccagcccga
     2521 tcacgacctc acaggcggcc acgaccatca cgatcagcgc gaacacctgg ccgtcgccgt
     2581 tgcccaccat gcgcgagaag gcgatcagcg ccaggttccc ggcgttcagc atcagctcca
     2641 ggcacagcag gatcaccagc gggctgcggc gggtgagcac gccggcggcg ccgatggcga
     2701 agatcagcgc ggacaggacg acgaaccact cgacgttcat cagtaggccc tcccgtcggg
     2761 gccgccgcgg gcgccgcggc cgggctggtc ggcgccctcc atcacgggct cacggacggt
     2821 gatggcgccc agcgcgctct cgggctcctc gagcccgcgc cgcttgcgcg ccatgaccac
     2881 ggcgccaacc gcggccacca gcagcaggaa cgaggccgcc tcgaagggca gcaggaagcg
     2941 gttgagcagc agctcgccga tctgcgcggg cgagccgaag ccctcgccga ccttcgggcc
     3001 ggtggtgtcc agcgcggaga gcgacgagcc cagcacggcc accgccagct ccaggaacag
     3061 gaccagggcg aacaccgggc ccagcacccg cagcacgccg ctctcgggcc gcagcggctc
     3121 gtccgcgccg ccgatgtagg ccacgacgaa caggtacagc accatcaccg ccccggcgta
     3181 gaccaccacc tgcgcggcgg ccaggaactc ggcccgcagc agcaggaaca gcaccgccag
     3241 ccccagcagg tgcgcgatca gcgacagcac cgaatagaac tggttgcgca gcgtcaccac
     3301 gccgatcgcc ccgcccaggg cggcgatgcc ggcgatgaag aacaggagct gctcgaagaa
     3361 catcgggcgg ggctactccc cctcgcgccg cagcggcgtg cgctccgggt ggtccgccag
     3421 cagcatctcc ttggtgaaga tcaggtccga gcggtcgtag tcggccatct cgtagtcgtt
     3481 gcccatggtg atggcgtcga acgggcaggc cacctcgcag tagccgcaga agatgcagcg
     3541 cgtcatgttg atctcgtaga ccgcggcgta gcgctcgccg gccgagacct ggttgtcggg
     3601 cgtgt
//
LOCUS       DASUPW010000285         9493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_66611, whole genome shotgun sequence.
ACCESSION   DASUPW010000285 DASUPW010000000
VERSION     DASUPW010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9493
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66611"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1359)
                     /locus_tag="VFY44_06460"
     CDS             complement(<1..1359)
                     /locus_tag="VFY44_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910118.1"
                     /translation="MIAVVLALAALYPGTAQAAETRYSLANGCFAVRSADTGAYVSRS
                     GSTRSLADAEPFRLKAATLGKYLLYGTKGDYLTPRGRRTAFTRRPSVWTVDGSVRSQD
                     GRFSFVRTGVCARFPEATAGAKGPAFKGATRLGEVSGTLDAHGHLTAYEFIGGKFHCG
                     KPWSPLGIQDALPDCSRDRGPGGAGAPLQNLLDFGSPVAGKDQVGYPTFKDWPSTHAL
                     TYEGTYWKWVERSWRSGLRMMVTLLVDNEVLCNLQTNRVNPCDDMQGAKLQRKRLYEL
                     QDYIDAQYGGPGKGFLRIVTDPFQARRVVADGKLAVVMGMETSALFGCRGSDQAPECD
                     QAKVDRGLDEFRKLGVRSFFPVHKFDNPLGGTKMDGGTTGLVVNPGNKVAFGKFWDAR
                     TCPGPETDNTQLTNGGAAAGALANAVQGTPLAGVLTGEAPVYPPGPHCNPRGLTPLGR
                     HLI"
     gene            complement(1395..2030)
                     /locus_tag="VFY44_06465"
     CDS             complement(1395..2030)
                     /locus_tag="VFY44_06465"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5910119.1"
                     /translation="MGAGAGSYEPSGRQVTAVEPSQAMIDQRPASAAPCIRAAAEALP
                     FADDSFDAALASMTVHHWDDLDRGLAELRRVARDRIVIFTWDPDVAAESWLTADYLPE
                     LRALDLFRFESPAELAERVGAATVTAVPVPADCRDGFIEAFWARPEAYLDPVVRAGMS
                     GMRAMDQEVVARAMERLEADLRSGEWDRRHGHLRDRAEMELGYRLVLAELR"
     gene            complement(2143..3252)
                     /locus_tag="VFY44_06470"
     CDS             complement(2143..3252)
                     /locus_tag="VFY44_06470"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX5910120.1"
                     /translation="MTVLRRTALCVLAVALPALAATQAGADPGAGATAKAKAKKPAPL
                     LIGHRGAAGYRPEHTLAGYTLAAEQGANYIEPDLVSTKDGVLVDRHEPEIGGTTNVAD
                     KPEFASRKRTKVVDGQTLTGWFTEDFTLAELRTLRAKERIPGVRPQNVKYDGRYQVPT
                     FQEVIDLSRRLTKRLKRPIGIYPETKHPTYFKKLGLPLEPKLVKILKKNKLTGKKAKV
                     FVQSFETAGLRQLNRQIDVPLVALMGAKDEKPADRPGTTYGKLMTPSGLKGLAKFVDG
                     IGPSKDSIIPLDASGRSAKPTSLVRDAHKAGLLVHPYTFRRENVFLPVELRSSADLNA
                     PGRLTDEIAKYLKLGVDGFFTDNPDLGRRAIRAAR"
     gene            complement(3299..3856)
                     /locus_tag="VFY44_06475"
     CDS             complement(3299..3856)
                     /locus_tag="VFY44_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX5910121.1"
                     /translation="MRFANQEEWREWLAAEHAAGPDGVWIEMAKKSSGIESITHPEAV
                     EVALCFGWIDSQRKGVDDQWFSQRFTPRRARSRWSRLMCEKAEELIASGAMQPAGLAE
                     VERAKADGRWDAAYEPPSTATVPGDLRAALDADPAAAGFFESLSGANRYAILHRIAEA
                     KRPETRARRIAKYVAMCAAGEKVHP"
     gene            complement(3853..4305)
                     /locus_tag="VFY44_06480"
     CDS             complement(3853..4305)
                     /locus_tag="VFY44_06480"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5910122.1"
                     /translation="MTAHDPQAPHTLTPNLVVDGGLEALEFYERAFGAEVVSRLEMDG
                     QLMHAELRVGNSGFTVSDEFADQGFVAPEEGRVNVSLSLWVEDCDAVHARAVEAGATG
                     TSAPADKFHGDRVGTIRCPFGHRWLIGTRIRDMSQEEMQKAMEDGLGG"
     gene            4368..5273
                     /gene="menA"
                     /locus_tag="VFY44_06485"
     CDS             4368..5273
                     /gene="menA"
                     /locus_tag="VFY44_06485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HEX5910123.1"
                     /translation="MASKAGLALQATRPLYIPTSLIPGLAGLAVAIHAPGVTWWLAPV
                     ALLALVLVHAGTNVINDVEDFQRGVDTEDKMDNSRVFNTGLMSLADGRRLAVGLFAAG
                     LALGVVIAAVQGPLILAFGVVGAGVGYGYSAGPRPLKYAGLGDVAIVPVMGPLITQGV
                     YTSVTGDAFHAAAFWLGLGPGLLIGAVLAANNLSDIPGDRAAGVRTLAVRLGFSRARA
                     GYLALLALPYATVAALVAAGLFGWPLLVALLTVPLAVQRGAQALAAPGEGDQGLVTLA
                     PGTAQLHLLFSLLLVAGVVIDRAVL"
     gene            5270..5824
                     /locus_tag="VFY44_06490"
     CDS             5270..5824
                     /locus_tag="VFY44_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910124.1"
                     /translation="MTGAADVPALLDGWIDDPQVRTHHRRASTADADRVWRAADELRL
                     SDTRTLGRLVRWRIPGTPPTLTFRELLRRYPFISLEEGPNHSLSGLCGRIWTLSRDYP
                     ELSGPDAFASWDESGTVRIVFATWVERGADGESVLCNEARIEPVDRRAGLRLRALWAA
                     IGRFEPLIGTEAVSRAVRQAEAGG"
     gene            complement(5803..6756)
                     /locus_tag="VFY44_06495"
     CDS             complement(5803..6756)
                     /locus_tag="VFY44_06495"
                     /inference="COORDINATES: protein motif:HMM:NF025608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HEX5910125.1"
                     /translation="MGRYRRLGADPPFGDPRRGHGIAMEGYYWRLSDPASGRVIVALC
                     GVCQSAEGPWAVVAVAAHPQGVLREGFAPDAWAEPGGYGVRAAGLLEAGDGRLSVDLG
                     PDTAIHAGLSDRAAWPRAAYGALGPAHWVPGLGQYWHPHLLDARAAGHATIAGEHVDL
                     TGWHVYGEKNWGSGFPRRWWWGQAHGFEREDVCVAFAGGRLPGAPVSATALVVRLGDE
                     LVRLSAPTALVRAGSTMTDWHVTGTGPRHRVELEGTAGADPFMLPVPLPAERRTEPGA
                     AQHLAGSVRLRVRRGRRLVYEGHSALAGLEHGDAAQPPASA"
     gene            complement(6764..8302)
                     /locus_tag="VFY44_06500"
     CDS             complement(6764..8302)
                     /locus_tag="VFY44_06500"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX5910126.1"
                     /translation="MNLLVAASKAPEIDYMGLSPVLALVGGAVLSLMVGLFPGRVVQR
                     GLVPLVGAASLLTAGGLSIANWETGDSNPIIAGALAIDTLALLLSLLFFVMGLATILL
                     SVRADVAREAGAGEYMTLLLSSILGMVILAGAENLVTLFIGLELLSIPLYVLCAAETR
                     RATSLESGLKYLVIGSVGSATLLYGLALVYGATGDTDFQGIADAVGDSVSVTDPLLLT
                     GVALVTAGLAFKASVAPFHQWTPDVYQGAPTPVTSFMAVATKVAAFAILLRFLDHALP
                     SAQTQWAPWLAVLAVTTILIGNTGAIAQDSLKRMLAWSGVAQAGYLLAGVVVGTRLGL
                     QAVCFYLIAYLFMNVAAFAVVTARERVSEHGDGLESLRDLGAANPLLAWPMTIAMLSL
                     AGFPATAGFIGKFYLIDAAVDGDYAWLGIAIVVGSMISLVYYLRVLAVMWMGSFAVEL
                     PTLPARTVKAVKGWSPEGDAKAQPEVVAVAVLAAAATVVFGIFPSPLFDLCRDAGTSL
                     SSML"
     gene            complement(8299..>9493)
                     /locus_tag="VFY44_06505"
     CDS             complement(8299..>9493)
                     /locus_tag="VFY44_06505"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX5910127.1"
                     /translation="RTKLYLFHLALGETAILGAFMAQDLALFVVFFDLMLVPFYFLIG
                     GWGHGDRVAATTKFVIFTLFGSLLMLAAAVALGVLATPDGGQLSYSLADLQRHTVGEG
                     TQNWIFLLFALAFLIKAPLFPFHGWVVDTYRATPTPVLVLLSAIMSKVGVYGFLRIVL
                     PILPDASVHFQDLILALAVFSILYGSILAFSQSEPRLVVAYSSIAQLGFIVLGIFSLD
                     PKGAQGALMQMVNHALVTAALFFIIAVLTARAKGPATLERLGGMAFRAPVLATLFLVV
                     ALATLAMPGTPNFVGEILILFGAMEDKLAFGVVASIGVALAAVYMIRIFQRSMHNREG
                     EGTDSRDVGAFDFAVIAPLAAVIVALGVYPQVALDPAEKATNAKVKPAERVADGKSNE
                     ISVIR"
BASE COUNT         1319 a         3456 c         3423 g         1295 t
ORIGIN      
        1 gatcagatgg cggcccagtg gcgtcagccc gcgcgggttg cagtgcgggc ccggcgggta
       61 gaccggcgcc tccccggtca ggaccccggc caggggcgtg ccctgcaccg cgttggccaa
      121 ggcgcccgcc gcggcgccgc cgttggtcag ctgcgtgttg tcggtctccg gcccggggca
      181 cgtgcgtgca tcccagaact tgccgaaggc caccttgttg cccgggttga ccaccagccc
      241 ggtggtcccg ccgtccatct tcgtgccgcc cagcgggttg tcgaacttgt gcacggggaa
      301 gaaggagcgg acgccgagct tgcgaaactc gtccaggccg cggtccacct tcgcctggtc
      361 gcactcgggc gcctggtcag agccgcggca gccgaacagc gccgaggtct ccatgcccat
      421 gaccaccgcg agcttgccgt cggccaccac gcgccgagcc tggaaggggt ccgtaacgat
      481 gcgcaggaag cccttgcccg ggccgccgta ctgggcgtcg atgtagtcct ggagctcgta
      541 caggcgcttg cgctggagct tcgcgccctg catgtcgtca cagggattta cgcggttggt
      601 ctgcaggttg cacaggacct cgttgtccac gagcagcgtg accatcatcc gcagccccga
      661 gcgccacgag cgctcgaccc acttccagta cgtgccctcg taggtcagcg cgtgggtgct
      721 gggccagtcc ttgaacgtcg ggtagcccac ctggtccttg ccggcgacgg ggctgccgaa
      781 gtcgagcagg ttctgcaacg gcgccccggc gccgccgggg ccgcggtccc tgctgcagtc
      841 gggcagggcg tcctggatcc ccagcgggct ccacggcttg ccgcagtgga acttgccgcc
      901 gatgaactcg taggcggtca ggtggccgtg ggcgtccagg gtgccggaga cctcgcccag
      961 ccgggtggcg cccttgaacg ccggcccctt ggcgccggcg gtggcctcgg ggaagcgcgc
     1021 gcacacgccg gtgcgcacga acgagaaccg gccgtcctgg gagcggacgg aaccgtcgac
     1081 cgtccacacc gacggcctgc gggtgaacgc cgtccgccgc ccgcgcggcg tcaggtagtc
     1141 gcccttggtg ccgtagagca ggtacttgcc cagcgtggcg gccttcagcc ggaagggctc
     1201 ggcgtcggcc aggctccggg tcgagccgct gcgcgacacg taggcaccgg tgtccgcgga
     1261 gcgcaccgcg aagcacccgt tggccagcga gtaccgcgtc tcggccgcct gggcggtgcc
     1321 ggggtacagg gcggccagcg ccagaaccac cgcaatcatc cagcctcgcc gcatccggaa
     1381 cgagtatgac ccccttaacg cagctcggct agtaccaacc ggtagcccag ctccatctcg
     1441 gcgcggtcgc gcaggtggcc gtggcgacgg tcccactcgc cgctgcgcag gtcggcctcc
     1501 agtcgctcca tcgcgcgggc caccacctcc tggtccatcg cccgcatgcc cgacatgccc
     1561 gcgcgcacca cgggatcgag gtaggcctcg gggcgggccc agaaggcctc gatgaagccg
     1621 tcgcggcagt cggccgggac gggcacagcc gtcacggtgg cggcgccgac gcgctcggcc
     1681 agctccgccg gcgactcgaa ccggaacagg tccagcgcgc gcagctccgg caggtagtcg
     1741 gcggtcagcc aggactcggc cgccacgtcg gggtcccagg tgaagatcac gatccggtcg
     1801 cgggccacgc gccgcagctc ggccaggccg cggtcgaggt cgtcccagtg gtgcacggtc
     1861 atcgaggcca gggccgcgtc gaacgagtcg tccgcgaacg gcagcgcctc ggccgcggcc
     1921 cgtatgcacg gcgccgccga cgccggccgc tggtcgatca tggcctgcga cggctcgacc
     1981 gcggtcacct gccggcccga cggctcgtag ctgccggccc cggcgcccac gttgaccacg
     2041 gtgcgcgcat cgcccagcgc ccggtcgatg gcggcggcga tgcgcgggtc ggcctcacgc
     2101 gtgcgcgcgt agccgtggcc tatgtcgtcg tagagcggcg tgtcagcgcg cggcccggat
     2161 cgccctgcga cccaggtcgg ggttgtcggt gaagaagccg tcgacgccga gcttgaggta
     2221 cttggcgatc tcgtcggtca gccgtcccgg cgcgttcagg tcggccgacg agcgcagctc
     2281 caccggcagg aacacgttct cgcgccggaa cgtgtacggg tgcacgagca gccccgcctt
     2341 gtgggcgtcg cgcacgagcg aggtgggctt ggccgacctt ccggacgcgt ccagcgggat
     2401 gatcgagtcc ttcgacggcc cgatgccgtc gacgaacttc gccaggccct tgaggcccga
     2461 cggcgtcatg agcttgccgt aggtcgtgcc cggccggtcg gcgggcttct cgtccttcgc
     2521 gcccatgagc gccaccagcg gcacgtcgat ctggcggttg agctggcgca gccccgcggt
     2581 ctcgaacgac tgcacgaaca ccttcgcctt cttgcccgtc agcttgttct tcttgaggat
     2641 cttcaccagc ttgggctcca gcggcaggcc cagcttcttg aagtaggtgg ggtgcttggt
     2701 ctcggggtag atcccgatcg ggcgcttgag ccgcttggta agccgccggc tcaggtcgat
     2761 gacctcctgg aaggtgggca cctggtaacg gccgtcgtac ttcacgttct gcggccggac
     2821 cccggggatg cgctccttgg cccgcagcgt gcgcagctcg gccagcgtga agtcctcggt
     2881 gaaccacccg gtcagcgtct gcccgtccac gaccttggtg cgcttgcggg aggcgaactc
     2941 gggcttgtcg gccacgttgg tggtgccgcc gatctccggc tcgtggcggt ccaccagcac
     3001 gccgtccttg gtcgagacca ggtcgggctc gatgtagttg gcgccctgct cggcggccag
     3061 ggtgtagccg gccagcgtgt gctcgggccg gtagcccgcc gcgccgcggt ggccgatcag
     3121 cagcggcgcc ggcttcttcg ccttggcctt ggccgtggcg ccggcgccgg ggtcggcgcc
     3181 cgcctgcgtg gcggccagcg cgggcagcgc gacggcgagg acacagaggg cggtgcggcg
     3241 caagacggtc atggccgcag cttatgcaca ccagggccac gcgacgacgc gcctgggcct
     3301 aggggtggac cttctcgccc gcggcgcaca tggccacgta cttggcgatc cgccgggcgc
     3361 gcgtctcggg ccgcttggcc tcggcgatcc ggtgcaggat cgcatagcga tttgcgccgg
     3421 acagggactc gaagaacccg gccgccgccg ggtccgcgtc cagtgccgcg cgcaggtcgc
     3481 cggggacggt ggcggtggac ggtggttcgt aggcggcgtc ccagcgcccg tcggccttgg
     3541 cccgctcgac ctcggccagc cccgcgggct gcatggcgcc cgaggcgatc agctcctcgg
     3601 ccttctcgca catcagccgt gaccaccggc tgcgtgcccg ccgcggggtg aagcgctggc
     3661 tgaaccactg gtcgtccacg cccttgcgct ggctgtcgat ccagccgaag cacagcgcca
     3721 cctcgaccgc ctcggggtgc gtgatcgact cgatgcccga ggacttcttg gccatctcga
     3781 tccacacgcc gtcgggaccg gcggcgtgct cggcggccag ccactcgcgc cactcttcct
     3841 ggttggcgaa cctcacccgc cgagcccgtc ctccatcgcc ttctgcatct cctcctggga
     3901 catgtcgcgg atgcgcgtgc cgatcagcca gcggtgcccg aaggggcagc ggatggtgcc
     3961 cacgcggtcg ccgtggaact tgtcggccgg ggccgaggtc ccggtggcgc cggcctccac
     4021 ggcgcgggcg tgcaccgcgt cgcagtcctc gacccagagc gagagcgaga cgttgacacg
     4081 gccctcctcc ggggccacga agccctggtc ggcgaactcg tcgctgacgg tgaacccgga
     4141 gttgcccacg cgcagctcgg cgtgcatgag ctggccgtcc atctccagcc gggacaccac
     4201 ctcggcgccg aacgcgcgct cgtagaactc cagcgcttcc agcccgccgt cgacgacgag
     4261 gttcggtgtg agcgtgtgcg gggcctgggg atcgtgtgcg gtcatggtct ccgcatcatg
     4321 aaggacggct gatcgggccc ggtgggcgga tccgtaccct cggcatcgtg gcttccaagg
     4381 ccggcctcgc cctccaggcg acgcgcccgc tctacatccc gacgtccctg atccccggcc
     4441 tcgcgggcct ggccgtggcc atccacgccc ccggcgtgac ctggtggctg gcgcccgtgg
     4501 cgttgctggc gctggtcctg gtgcacgcgg gcacgaacgt gatcaacgac gtcgaggact
     4561 tccagcgcgg cgtggacacc gaggacaaga tggacaactc gcgcgtgttc aacacggggc
     4621 tgatgtcgct ggcggacggc cgccgcctgg ccgtcgggct gttcgccgcc gggctggcgc
     4681 tgggcgtggt catcgccgcc gtccaggggc cgctgatcct ggcgttcggc gtggtcggcg
     4741 cgggcgtggg ctacgggtac tcggccggcc cccggccgct gaagtacgcg ggcctgggcg
     4801 acgtggccat cgtgcccgtc atggggccgc tgatcacgca gggcgtctac acctcggtca
     4861 ccggcgacgc attccacgcg gccgcgttct ggctcggcct cggccccggg ctgctgatcg
     4921 gcgccgtgct ggcggccaac aacctgtccg acatcccggg cgaccgcgcc gccggtgtgc
     4981 gcacgctggc ggtgcggctg ggcttctccc gcgcccgggc ggggtacctg gcgctgctgg
     5041 cgttgcccta cgccaccgtg gccgcgctcg tggcggcagg gctcttcggc tggccgcttc
     5101 tggtcgcgct gctcaccgtg ccgctggccg tccagcgtgg cgcccaggcc ctggccgccc
     5161 cgggcgaggg cgatcagggc ctggtcacgc tggcccccgg caccgcccag ctgcacctgc
     5221 tgttcagcct gctgctggtc gccggagtgg tgatcgaccg ggcggtgctg tgaccggggc
     5281 cgccgacgtg cccgccctgc tcgacgggtg gatcgacgat ccgcaggtgc gcacccacca
     5341 ccggcgcgcg agcacggccg acgccgacag ggtgtggcgc gccgccgacg agctgcgcct
     5401 gtcggacacc cggacgctgg gccggctggt gcgctggcgc atcccgggca cgccgcccac
     5461 gctcaccttc cgcgagctcc tgcgccggta cccgttcatc tccctcgagg aggggcccaa
     5521 ccactcgctc tcggggctgt gcgggcgcat ctggacgctc agccgcgact accccgagct
     5581 gagcgggccg gacgcgttcg cctcgtggga cgagtcgggc acggtgcgga tcgtgttcgc
     5641 cacgtgggtg gagcggggtg ccgacggcga gtcggtgctc tgcaacgagg cccgcatcga
     5701 gcccgtcgac cggcgggcgg gcctgcgcct gcgcgcgttg tgggcggcca tcggccggtt
     5761 cgagccgctg atcggcaccg aggcggtctc gcgtgccgtg cgtcaggccg aggcgggcgg
     5821 ctgagccgcg tcgccgtgct ccagcccggc cagcgcgctg tggccctcgt acaccagccg
     5881 ccggccgcgc cgcacgcgta ggcgcacgct gccggccagg tgctgggccg caccgggctc
     5941 ggtgcggcgc tcggcgggga gcggcaccgg gagcatgaag gggtccgctc cggccgtgcc
     6001 ctccagctcg acccggtggc gcgggccggt gcccgtcacg tgccagtcgg tcatggtgct
     6061 gccggcgcgc accagcgccg tgggggccga cagccggacc agctcgtcgc ccagccgtac
     6121 caccagcgcg gtggccgaga ccggcgcacc cggcaaccgc ccgccggcga acgccacgca
     6181 cacgtcctcg cgctcgaagc cgtgggcctg accccaccac caccggcgcg ggaagccgct
     6241 gccccagttc ttctccccgt agacgtgcca gccggtcagg tccacgtgct cgccggcgat
     6301 ggtcgcgtgg cccgcggcgc gcgcgtccag caggtgcggg tgccagtact ggcccaggcc
     6361 ggggacccag tgcgccggtc ccagggcgcc gtacgccgcg cgcggccacg ccgcacggtc
     6421 gctcagcccg gcgtggatgg ccgtgtcggg tcccaggtcc accgacagcc ggccgtcgcc
     6481 ggcctcgagc agcccggcgg cacggacccc gtagccgccc ggctcggccc acgcgtccgg
     6541 cgcgaagccc tcgcgcagca ccccctgtgg gtgggccgcc acggcgacca ccgcccacgg
     6601 cccctcggcg ctctggcaca ccccgcacag ggcgacgatc acacggcccg acgcgggatc
     6661 gctcaggcgc cagtagtagc cctccatcgc gatcccgtga ccgcgccgcg ggtcgccgaa
     6721 cggcgggtcc gctccgaggc ggcggtaacg ccccaccgtc gccctagagc atcgagctga
     6781 gcgacgtgcc cgcgtcgcgg cacaggtcga acaggggtga cgggaagatc ccgaacacca
     6841 cggtcgccgc ggcggccagg acggccaccg ccaccacctc gggctgggcc ttcgcgtcgc
     6901 cctcgggcga ccagcccttg acggccttga ccgtgcgcgc gggcagggtc ggcagctcga
     6961 ccgcgaagct gcccatccac atcacggcca gcacgcgcag gtagtagacc agcgagatca
     7021 tggacccgac gacgatggcg atgcccagcc acgcgtagtc gccgtccacg gcggcatcga
     7081 tcaagtagaa cttcccgatg aagccggcgg tggcggggaa gccggccagc gacagcatcg
     7141 cgatggtcat gggccaggcc aggagcgggt tcgccgcgcc caggtcgcgc agcgactcca
     7201 gcccgtcgcc gtgctcggag acgcgctcgc gcgcggtgac cacggcgaag gcggccacgt
     7261 tcatgaacag gtaggcgatc aggtagaagc agaccgcctg caggcccagc cgggtgccca
     7321 ccacgacgcc ggccagcagg tagccggcct gggccacgcc ggaccaggcc agcatccgct
     7381 tgagcgagtc ctgggcgatg gcgccggtgt tgccgatcag gatcgtggtc acggccagca
     7441 ccgccagcca cggggcccac tgggtctggg ccgacggcag cgcgtggtcc aggaagcgca
     7501 gcaggatcgc gaacgcggcg accttggtgg ccacggccat gaagctggtg accggggtgg
     7561 gcgcgccctg gtagacgtcg ggcgtccact ggtggaacgg cgcgaccgac gccttgaagg
     7621 ccaggcccgc cgtgaccagc gccacgcccg tgagcagcag cgggtcggtc accgagacgc
     7681 tgtcgcccac cgcgtcggcg atgccctgga agtcggtgtc gccggtggcg ccgtagacca
     7741 gcgccagccc gtacagcagc gtggccgagc ccaccgatcc gatgaccagg tacttcaggc
     7801 ccgactccag cgaggtggcc cggcgagtct cggcggcgca cagcacgtac agcgggatcg
     7861 acagcagctc caggccgatg aacagcgtga ccaggttctc ggcgccggcc aggatcacca
     7921 tgcccaggat cgagctcagc agcagggtca tgtactcgcc cgcgccggcc tcacgcgcca
     7981 cgtcggcgcg caccgacagc aggatcgtgg ccaggcccat gacgaagaac agcaggctca
     8041 gcagcagcgc cagcgtgtcg atggccagcg caccggcgat gatcgggttg ctgtccccgg
     8101 tctcccagtt ggcgatgctc agcccgcccg cggtgagcag cgacgcggcg cccaccagcg
     8161 ggaccagccc gcgctggacc acgcggcccg ggaacaggcc gaccatgagc gacaggaccg
     8221 cgccgcccac cagggccagc acgggcgaca gccccatgta gtcgatctcg ggcgccttgg
     8281 aggcggcgac cagcaggttc atcggatcac cgagatctcg ttcgacttgc cgtcggccac
     8341 ccgctcggcg ggcttgacct tcgcgttcgt ggccttctcg gccgggtcca gcgccacctg
     8401 cgggtacacg cccagcgcga cgatcacggc cgccagcggc gcgatcacgg cgaagtcgaa
     8461 cgcgccgacg tcacgcgagt cggtgccctc gccctcgcgg ttgtgcatcg agcgctggaa
     8521 gatccggatc atgtagacgg cggccagcgc gaccccgatc gaggccacga cgccgaacgc
     8581 cagcttgtcc tccatggcgc cgaacaggat caggatctcg ccgacgaagt tcggggtgcc
     8641 gggcatggcc agcgtggcca gggcgaccac caggaacagc gtggccagca cgggcgcgcg
     8701 gaaggccatg ccgcccaggc gctccagcgt ggccgggccc ttggcccgcg ccgtcagcac
     8761 cgcgatgatg aagaacaggg ccgccgtgac cagggcgtgg ttgaccatct gcatcagcgc
     8821 gccctgggcg cccttggggt ccaggctgaa gatgcccagg acgatgaagc ccagctgcgc
     8881 gatcgaggag taggccacca ccagccgcgg ctcggactgg gagaaggcca ggatcgaccc
     8941 gtacaggatc gagaacacgg ccagcgccag gatcaggtcc tggaagtgca cggaggcgtc
     9001 gggcaggatc ggcagcacga tccgcaggaa gccgtagacg ccgaccttgg acatgatcgc
     9061 gctcagcagc accagcaccg gggtcggcgt ggcgcggtag gtgtccacca cccagccgtg
     9121 gaacgggaac agcggcgcct tgatcaggaa cgcgagcgcg aacagcagga agatccagtt
     9181 ctgggtgccc tcgccgaccg tgtgccgctg gaggtcggcc agcgagtagg acagctggcc
     9241 gccgtcgggc gtggccagca cgcccagcgc gaccgccgcc gccagcatca gcagcgagcc
     9301 gaacagggtg aagatcacga acttggtggt ggccgccacc cggtcgccgt ggccccagcc
     9361 gccgatcagg aagtagaacg gcaccagcat caggtcgaag aacacgacga acagcgccag
     9421 gtcctgcgcc atgaacgcgc ccaggatggc ggtctcgccc agcgccaggt ggaacaggta
     9481 gagcttggtg cgc
//
LOCUS       DASUPW010000286         3261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1589877, whole genome shotgun sequence.
ACCESSION   DASUPW010000286 DASUPW010000000
VERSION     DASUPW010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589877"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /gene="lhgO"
                     /locus_tag="VFY44_06510"
     CDS             <1..912
                     /gene="lhgO"
                     /locus_tag="VFY44_06510"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HEX5910128.1"
                     /translation="AYQRCGKLIVAKDASEVPALDELERRATENGVPGLRRLTGDQIA
                     EVEPHCVGEAALHSPNTGIVDFAAVARAMGDDLRAAGGTVTTGAGVEAIWTTAEGIGV
                     RHAAGQESARFLVSCAGLASDRVAVLAGADPDPRIVPFRGAYLRLRPDKRDLVKGLIY
                     PVPDPSLPFLGVHLTLHVDGEVSLGPTALLAPRLSSLGWPGTLRMARRWWRTGLAEMR
                     SAVRTSEFAREAAQYVPELEPGDFSPYFAGYRAQALGRDGTLVDDFVIHETPNALHVR
                     NAPSPAATSSLAIADMIASRAREALAA"
     gene            complement(909..1568)
                     /locus_tag="VFY44_06515"
     CDS             complement(909..1568)
                     /locus_tag="VFY44_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910129.1"
                     /translation="MSRRALAAFVAVPVVVALVGCLVAAAGNQRDLVLRGNGFAGEVV
                     PVKTGSLACQAELEPAEAFDGVRFGVAGDRALAGPPVTVTVKRGTRTLARGDLAPGYL
                     GQAKRLVELDRAVQPQDDLRVCFFNRGGRRLLLYGTPTAARGPDVTIDGKAKELGLQI
                     DLTREPRSALSMLGTVADRASLQRPGPAGPWLVWLLAAVVLVAVPAVAVGAVRAATPP
                     D"
     gene            complement(1561..3003)
                     /locus_tag="VFY44_06520"
     CDS             complement(1561..3003)
                     /locus_tag="VFY44_06520"
                     /inference="COORDINATES: protein motif:HMM:NF021436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEX5910130.1"
                     /translation="MPRPLGLLLVCVTLLGVAWAWFTPPWEGPDETMHYGYVETLAQR
                     HTRPGVERGGNVLSTGQRLSMRGSNADRLTHISDGRPQWSEAAARPFEQRSDVQRTDD
                     GGFATKEGHVNTAKGNPPGFYVLELPAYAAGAAVGGVNSRLYAMRMFTIASLLLGVVA
                     VWLLIGELVPRQPLAQLAGAASVGLLPMSTHIAASVNPDALLYSLWAVVLWLGVRVLK
                     RGLTLRSGLALVGALVAALAVKATAVAVAPPVLGVLAYGLWKRPDVRARLGSLGRTRV
                     RRVGLGLGTVVVGSGLAFVAATRYTSQLTEQLRGQFEPGLMASYLWQFYLPRLGFMQD
                     IKTVTGIPVYDVWFKDLVGNFAWLQISQPGWLYAVLAVLTVAVAAVGLVALWRDQPRS
                     VVVFFAAVTVVLLAGLHITDYRQAVVTGIGFMQGRYLLPLAGIFAAVLVAAWTRLPER
                     RRPQATALLLGGLLALQIVSFSRAAGFWYV"
BASE COUNT          469 a         1248 c         1172 g          372 t
ORIGIN      
        1 gcctaccagc gctgcggcaa gctcatcgtc gccaaggacg cctcggaggt cccggcgctg
       61 gacgagctgg agcggcgcgc caccgagaac ggcgtgcccg gcctgcgccg gctgaccggc
      121 gaccagatcg ccgaggtcga gccccactgc gtcggcgagg cggcgctgca ctcgcccaac
      181 accggcatcg tggacttcgc cgccgtggcc cgcgcgatgg gcgacgacct gcgcgcggcc
      241 ggcgggacgg tgaccaccgg cgcgggggtg gaggccatct ggaccaccgc ggagggcatc
      301 ggggtgcggc acgcggcggg acaggagagc gcgcgcttcc tggtctcctg cgcggggctg
      361 gcctccgacc gggtggcggt gctggccggc gccgaccccg atccccggat cgtccccttc
      421 cgcggcgcct acctgcgcct gcgcccggac aagcgcgacc tcgtgaaggg cctgatctac
      481 ccggtgccgg acccgtcgct gcccttcctg ggcgtgcacc tgacgctgca cgtggacggg
      541 gaggtgtcgc tgggcccgac cgcgctgctg gcgccgcgcc tgtccagcct cgggtggccc
      601 ggcacgctgc ggatggcgcg gcgctggtgg cgcaccggcc tggccgagat gcggagcgcg
      661 gtccgcacca gcgagttcgc ccgggaggcg gcccagtacg tgccggagct cgagccgggc
      721 gacttcagcc cgtacttcgc cggctaccgc gcccaggccc tgggccgtga cgggacgctg
      781 gtcgacgact tcgtgatcca cgagaccccg aacgccctgc acgtgcgcaa cgcaccgtcg
      841 ccggcggcca cgtcgtcgct ggcgattgcg gacatgatcg cctcgcgggc ccgcgaggcg
      901 ctggccgcct agtccggcgg ggtcgccgcg cgcaccgcgc ccacggccac ggccgggacg
      961 gccaccagca ccacggcggc caggagccag accagccacg ggccggcggg gcccgggcgc
     1021 tgcagggagg cgcggtccgc caccgtgccc agcatcgaca gcgcggagcg cggctcccgc
     1081 gtcaggtcga tctgcaggcc gagctccttg gccttgccgt cgatggtcac gtccggtccg
     1141 cgggcggcgg tgggggtgcc gtagagcagc agcctccggc cgccccggtt gaagaagcac
     1201 accctcaggt cgtcctgggg ctgcacggcg cggtccagct cgaccagccg cttcgcctgt
     1261 cccaggtacc cgggcgccag gtcgccgcgg gccagcgtcc gggtgccccg cttgaccgtg
     1321 accgtcaccg gtgggccggc cagggcgcgg tcacccgcca cgccgaagcg cacgccgtcg
     1381 aacgcctccg cgggctccag ctcggcctgg cacgccaggc ttcccgtctt gaccgggacc
     1441 acctcgccgg cgaaaccgtt gccgcgcaac accaggtcgc gctggttgcc cgccgccgcc
     1501 accaggcagc ccaccagggc cacgacgacc ggcaccgcca cgaatgcggc cagggcgcgc
     1561 ctagacatac cagaagcccg ccgcgcggct gaaggacacg atctggaggg ccagcaggcc
     1621 gcccaggagg agcgcggtcg cctggggccg gcgccgctcc ggcagccggg tccacgccgc
     1681 caccagcacc gcggcgaaga tcccggccag cggcaacagg tagcggccct gcatgaaccc
     1741 gatgccggtg accaccgcct gccggtagtc ggtgatgtgc aggccggcca gcagcaccac
     1801 ggtcaccgcg gcgaagaaca cgacgacgct ccggggctgg tcgcgccaca gggccaccag
     1861 gcccacggcg gcgacggcca cggtgagcac ggcgaggacg gcgtacagcc agcccggctg
     1921 gctgatctgc aaccaggcga agttcccgac caggtccttg aaccagacgt cgtagaccgg
     1981 gatcccggtc accgtcttga tgtcctgcat gaagcccagg cggggcaggt agaactgcca
     2041 caggtagctg gccatcagcc ccggctcgaa ctggccgcgc agctgctcgg tcagctggga
     2101 cgtgtaccgg gtggccgcca cgaacgcgag acccgagccg acgacgaccg tccccagccc
     2161 gagcccgacg cggcgcaccc gcgtccggcc cagggacccc aggcgggccc ggacgtcggg
     2221 gcgcttccac agcccgtagg ccagcacccc caggacgggc ggcgcaaccg cgaccgccgt
     2281 ggccttgacc gcgagtgcgg cgacgagcgc tcccacgagc gccagccccg accgcagggt
     2341 gagcccgcgt ttgagcaccc tcacgcccag ccacaggacg accgcccaca acgagtacag
     2401 caacgcgtcc gggttcaccg acgcggcgat gtgggtcgac atgggcagca ggcccacgga
     2461 cgcggcgccc gcgagctggg ccagcggctg cctgggcacc agctccccga tcagcagcca
     2521 gacggccacc acgcccagca gcaacgaggc gatcgtgaac atccgcatcg cgtacaggcg
     2581 gctgttcacg ccaccgaccg cggcgccggc ggcgtacgcg ggcagctcga gcacgtagaa
     2641 cccgggcggg ttgcccttgg ccgtgttcac gtgcccctcc ttggtggcga accctccgtc
     2701 gtcggtgcgc tgcacgtcgc tgcgctgctc gaagggccgg gccgccgcct cggaccactg
     2761 ggggcggccg tcggagatgt gcgtgaggcg gtcggcgttg gagccccgca tcgacaggcg
     2821 ctggcccgtg gacagcacgt tgccgccccg ctcgacgccg ggccgcgtgt ggcgctgggc
     2881 cagcgtctcc acgtagccgt agtgcatggt ctcgtccggg ccctcccagg gcggcgtgaa
     2941 ccacgcccac gcgaccccca ggagggtcac gcacaccagc agcagcccca ggggacgcgg
     3001 caccctcgta cgtggatcag gccccatgcg acggggaacc ctagagcgcg cccaaggtcg
     3061 cggtggcgct ctggcgcgta gcctccggcg ccgtatgcgc cttatcgaca ccaccctgga
     3121 cggaccactg ctgatcgagc ccgtcgtgca cggcgaccag cgcggcttct tcgtggagag
     3181 cttccgcgcc aacgtgatgg ccgacctggg catcgccgac gagttcgtgc aggacaacca
     3241 ttcgcgctcc gcgcgcggcg t
//
LOCUS       DASUPW010000287         2231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_898529, whole genome shotgun sequence.
ACCESSION   DASUPW010000287 DASUPW010000000
VERSION     DASUPW010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898529"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VFY44_06525"
     CDS             complement(<1..292)
                     /locus_tag="VFY44_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910131.1"
                     /translation="MAGSSTADQPVFYYDLGSPYAYLTAERINHVLGVVPVWQPILLG
                     GIWQKTGGDSWARTDAREEGMAEIERRAESYGMQSIRWPDPWPTNTLTAMRVA"
     gene            complement(270..1655)
                     /gene="lpdA"
                     /locus_tag="VFY44_06530"
     CDS             complement(270..1655)
                     /gene="lpdA"
                     /locus_tag="VFY44_06530"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX5910132.1"
                     /translation="MAETKYDCIVIGTGPGGYVAAIRAAQLGMKTAVVEKADTVGGRC
                     LNEACIPAKAVLRVADAMKEVQGAKSFGITTGDVSFDFGGATKHRDKVIKTLTGGVAM
                     LFKKNGIDVIEGHGTVTDDGNVKVGGSFDGTEIEAGKVILATGSIARPVGGLEFGGRI
                     VDTAKMWLTAEQPKRLAVIGAGASGTEVASAFGRLGTDVILLEALDQILPLEEPEIAK
                     ATAKEIGKQNVKIITGANVENVDDSGDAVKIDVGGETHEVDLVCIAAGRGADVEGLGL
                     EDAGIETDDRGLIKVDGALRTSKEGVYAIGDLVPGPALAHKASDEGVIAVEDAAGNDT
                     HPIDYAYVPRVTFCAPQVASFGLTEAQAKEQGYDVVVGKVPMGAVGAPTVYGDRGGMV
                     KMIGDKKYGEILGAHIVSAKAAELIQELVVARDLEGGYPEIARSIHPHPAFAEVILEA
                     ARATDGWLIHG"
     gene            1677..1937
                     /locus_tag="VFY44_06535"
     CDS             1677..1937
                     /locus_tag="VFY44_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910133.1"
                     /translation="MSTLHYVVEVSDRRNAQLTGSGGGTYRSPPHTLLHAHELVAVLL
                     DFPGVPIEGEGPWRRAVPGGERVVSLVLADRLFDPGAAPPPS"
     gene            complement(1934..>2231)
                     /locus_tag="VFY44_06540"
     CDS             complement(1934..>2231)
                     /locus_tag="VFY44_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910134.1"
                     /translation="LAVNFDSSKGPQTTFTILACVLLTAMAYGMWHARYWAVLGMQTL
                     LGITVLLASLGLISAVNLWAALLLVLIIVGAGTLFWFLVKAMARIQMPERPGAR"
BASE COUNT          289 a          811 c          772 g          359 t
ORIGIN      
        1 tggccacgcg catggcggtc agcgtgttcg tgggccacgg gtcgggccag cggatgctct
       61 gcatcccgta gctctccgcg cggcgctcga tctcggccat gccttcctcg cgggcgtcgg
      121 tgcgcgccca cgagtcgccg ccggtcttct gccagatgcc cccgagcagg atcggctgcc
      181 acaccgggac gacgcccagg acgtggttga tccgctcggc cgtcaggtac gcgtagggcg
      241 acccgaggtc gtagtagaag accggctggt cagccgtgga tgagccagcc atcggtggcg
      301 cgcgccgcct ccaggatgac ctcggcgaac gccgggtgcg ggtggatcga gcgggcgatc
      361 tcggggtagc cgccctcgag gtcgcgggcc accacgagct cctggatcag ctccgcggcc
      421 ttggcggaca cgatgtgcgc gcccaggatc tcgccgtact tcttgtcgcc gatcatcttg
      481 accatgccgc cgcggtcgcc gtagaccgtg ggggcgccga cggcgcccat cggcaccttg
      541 ccgaccacca cgtcgtagcc ctgctccttg gcctgggcct cggtcaggcc gaagctggcc
      601 acctgcgggg cgcagaaggt cacgcgcggg acgtacgcgt agtcgatcgg gtgggtgtcg
      661 ttgccggcgg cgtcctcgac ggcgatcacg ccctcgtcgg acgccttgtg cgccagggcg
      721 ggcccgggga ccaggtcgcc gatggcgtag acgccctcct tcgaggtgcg cagcgcgccg
      781 tcgaccttga tcagcccgcg gtcgtcggtc tcgatgccgg cgtcctccag gcccaggccc
      841 tccacgtcgg cgccgcgccc ggcggcgatg cacaccaggt cgacctcgtg ggtctcgccg
      901 cccacgtcga tcttcaccgc gtcgccggag tcgtccacgt tctcgacgtt ggcgccggtg
      961 atgatcttca cgttctgctt gccgatctcc ttggccgtgg ccttggcgat ctccggctcc
     1021 tccagcggca ggatctggtc cagcgcctcg agcaggatca cgtcggtgcc caggcggccg
     1081 aaggcggacg cgacctcggt gccggacgcg ccggcgccga tcacggcgag gcgcttgggc
     1141 tgctcggccg tgagccacat cttggccgtg tccacgatcc ggccgccgaa ctccaggccg
     1201 ccgaccggcc gggcgatcga gccggtggcc aggatgacct tgccggcctc gatctcggtg
     1261 ccgtcgaacg agccgccgac cttgacgttg ccgtcgtcgg tcacggtgcc gtggccctcg
     1321 atcacgtcga tgccgttctt cttgaacagc atcgccacgc cgccggtgag ggtcttgatg
     1381 accttgtcgc ggtgcttggt ggcgccgccg aagtcgaacg acacgtcgcc ggtggtgatg
     1441 ccgaagctct tggccccctg gacctccttc atcgcgtcgg ccacgcgcag cacggccttc
     1501 gcggggatgc acgcctcgtt caggcagcgc cccccgacgg tgtccgcctt ctccaccacg
     1561 gcggtcttca tgcccagctg ggcggcgcgg atcgcggcca cgtagccgcc gggtccggtg
     1621 ccgatgacga tgcagtcgta cttggtctct gccatttcaa gcgagactat cgggcagtgt
     1681 ccacgctcca ctacgtcgtc gaggtctccg atcgccgcaa cgcccagctc acgggcagcg
     1741 gcggcgggac ctaccgcagc ccgccccaca cgctcctgca cgcgcacgag ctcgtggccg
     1801 tgctgctgga cttccccggc gtgccgatcg agggcgaggg gccgtggcgg cgggccgtgc
     1861 cgggcggcga gcgtgtggtc tcgctggtgc tggccgaccg cctgttcgac ccgggggcgg
     1921 ctccgccgcc ctcctagcgc gcgccgggcc gctcgggcat ctggatccgg gccatggcct
     1981 tgaccaggaa ccagaacagc gtgccggcgc cgacgatgat cagcaccagc agcagcgcag
     2041 cccacaggtt caccgccgag atcaggccca gcgaggccag cagcaccgtg atgcccagca
     2101 gggtctgcat gcccagcacc gcccagtagc gggcgtgcca catcccgtag gccatggcgg
     2161 tcagcagcac gcaggccagg atcgtgaacg tggtctgcgg ccccttgctg gagtcgaagt
     2221 tcaccgccag c
//
LOCUS       DASUPW010000288         2562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1037527, whole genome shotgun sequence.
ACCESSION   DASUPW010000288 DASUPW010000000
VERSION     DASUPW010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037527"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VFY44_06545"
     CDS             complement(<1..414)
                     /locus_tag="VFY44_06545"
                     /inference="COORDINATES: protein motif:HMM:NF023275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-(1->3)-arabinofuranosyltransferase family
                     protein"
                     /protein_id="HEX5910135.1"
                     /translation="MRDPRSRAIPLGLAAAAMLAALLQRPGETSADTKIDLHVDPVGF
                     LGDVMSVWTNSGALGEVQGGQYAGYLFPMGPFFALGHLLGLPDWLVDRLWIGVILALA
                     AWGTVRLLDALMPGPRGAAHVVAGALMVFNPYTVVY"
     gene            complement(411..1670)
                     /locus_tag="VFY44_06550"
     CDS             complement(411..1670)
                     /locus_tag="VFY44_06550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5910136.1"
                     /translation="MRGRLKGFAGPLVLIVVAGLAIRVAYILLLGERVGGIGDYFFYD
                     TSANLLAEGKGFIDPFSLATGPARPTAEHPPLWSMVLSVTSWLGGTGFTSHKLTGAVV
                     GAAAIVPIALLGRRVAGERAGLIAAVLAAVYPTFIASDGSLMSESLYGLLVACALVAA
                     YRLYDRADLVSAAALGVFLGLSALTRAEGILLLGLLVVPLCWRLPTDVRARVVRFGVV
                     VAAAVVVIAPWTARNWIQFDRPVLISINDAAVLAGANCDQTYHGRDIGLWRVDCASRK
                     KGATQPEQADLWREKGLDYAGEHPGRLPAVVAVRVLRVWDFWAPYDQTLAEGRQRDVQ
                     VAGTAMYFLLLALAVYGVVLLRRRDQPLLILAAPVVVVTLSAMVGYGLPRFRQAAEVV
                     IVVLAAAALAELATRARERRRPRKIGA"
     gene            1729..>2562
                     /locus_tag="VFY44_06555"
     CDS             1729..>2562
                     /locus_tag="VFY44_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910137.1"
                     /translation="MAAVVAILAGPVALAFFSGGFFDLPRLWTAAGAWLLVLLATIAA
                     PRPLPASTSGRCALAGLALLTAWTAVSLAWAPLRAPAVDDVQRLVLYLGGLLAACAFL
                     RGERALRAVEPGVAAGTVVATLYGLSERLLPGLFELTRSASAEGRLDQPLTYWNAMGA
                     LTAIGLVLCARLGGDDSRPAAVRAAAAAAAPVLGLGLFLTLSRGAIATAAAGLLVLVV
                     LCPTRLQLRAAVLTLVSGGVAAAAALALPAVRTLEAGQSARDGQGLAMLGVLLVVAAA
                     SA"
BASE COUNT          330 a         1002 c          923 g          307 t
ORIGIN      
        1 gtagaccacc gtgtacgggt tgaacaccat cagcgctccc gccaccacgt gcgccgcgcc
       61 gcgcggcccg ggcatcagcg cgtccagcag tcgcaccgtc ccccaggcgg ccaaggccag
      121 gatcacgccg atccagagcc ggtccaccag ccagtcgggc agcccgagca ggtggcccag
      181 ggcgaagaac ggccccatcg ggaacaggta gcccgcgtac tggccgccct gcacctcgcc
      241 gagcgcgccg ctgttggtcc acacggacat cacgtcgccc aggaagccga cggggtccac
      301 gtggaggtcg atcttggtgt cggccgaggt ctcccccggc cgttggagca gcgccgcgag
      361 catcgccgcg gccgccaggc ccagcgggat cgcccgcgac cgcgggtccc tcacgcgccg
      421 atcttccgcg gccggcgacg ctcccgggcc cgggtggcca gctcggccag ggcggcggcc
      481 gccaggacca cgatcacgac ctcggccgcc tggcggaacc gcggcagccc gtagcccacc
      541 atggccgaca gcgtgaccac caccaccggc gccgccagga tcagcagcgg ctggtcgcgg
      601 cggcgcagca gcacgacgcc gtacaccgcc agcgccagca gcaggaagta catcgcggtg
      661 ccggccacct gcacgtcacg ctgacggccc tcggccagcg tctggtcgta cggcgcccag
      721 aagtcccaga cccgcagcac ccggaccgcc accaccgccg gcagccggcc ggggtgctcg
      781 ccggcgtagt ccagcccctt ctcgcgccac aggtcggcct gctcgggctg cgtcgcgccc
      841 ttcttgcgcg aggcgcagtc cacccgccac agcccgatgt cgcggccgtg gtaggtctgg
      901 tcgcagttgg ccccggccag caccgcggcg tcgttgatcg agatcagcac gggacggtcg
      961 aactggatcc agttgcgcgc cgtccagggc gcgatgacca ccacggccgc ggccaccacc
     1021 acgccgaagc ggaccacccg cgcgcgcacg tcggtgggca ggcgccagca cagggggacc
     1081 acgagcaggc ccagcagcag gatgccctcc gcccgcgtca gcgcggacag ccccaggaac
     1141 acccccagcg cggccgcgct gaccaggtcg gcgcggtcgt acaggcggta ggcggccacc
     1201 agcgcgcacg ccaccagcag gccgtagagc gactcgctca tcagcgaccc gtcggaggcg
     1261 atgaacgtgg ggtacacggc ggccagcacg gccgcgatca ggcccgcccg ctcaccggcg
     1321 accctccggc ccagcagcgc gatcgggacg atggccgccg cccccacgac ggccccggtg
     1381 agcttgtgcg aggtgaaccc cgtgcccccc agccacgacg tcaccgacag caccatcgac
     1441 cacagcggcg ggtgctcggc ggtcggccgc gcgggcccgg tggccagcga gaacgggtcg
     1501 atgaagccct tgccctcggc cagcaggttg gccgacgtgt cgtagaagaa gtagtcgccg
     1561 atcccgccga cgcgctcccc cagcaggagg atgtaggcaa ccctgatcgc caggcccgcg
     1621 accacgatca ggacgagcgg gcccgcgaag cctttgagcc gaccgcgcat gcagcgccgc
     1681 agcgtagtat cagcctccgt cttgcgcgta ccgacgactt cagtccgcgt ggccgcggtc
     1741 gtcgccatcc tggccgggcc ggtggccctg gccttcttct cgggcgggtt cttcgatctg
     1801 ccgcggttgt ggacggcggc gggcgcctgg ctgctggtgc tcctggcgac catcgccgca
     1861 ccccgcccgc tgccggcgag cacgtccggc cgctgcgccc ttgccggcct ggcgctgttg
     1921 acggcctgga ccgcggtctc gctggcctgg gcgccgctgc gcgcgccggc cgttgacgac
     1981 gtccagcggc tggtgctgta cctgggcggc ctgctggccg cgtgcgcgtt cctccgcgga
     2041 gagcgagcgc tgcgggcggt cgagcccggc gtggccgcgg gcaccgtcgt cgcgacgctc
     2101 tacggcctct cggagcggct gctcccgggc ctgttcgagc tcacgcgcag cgccagcgcc
     2161 gagggccgcc tggaccagcc gctgacctac tggaacgcca tgggggcact gaccgcgatc
     2221 ggcctggtcc tgtgcgcgcg gctgggcggc gacgacagcc gcccggcggc ggtgcgcgcg
     2281 gcggcggccg ccgccgcccc ggtgctgggg ctcgggctgt tcctgacgct ctcgcgtggg
     2341 gcgatcgcca cggccgcggc cggcctgctg gtgctggtcg tgctctgccc cacccgcctc
     2401 cagctgcgtg cggccgtgct gaccctggtg tcggggggcg tcgccgccgc cgcggcgctg
     2461 gcgctgcccg cggtgcgcac cctggaggcc ggccagtcgg cgcgggacgg ccagggcctg
     2521 gccatgctgg gcgtgctgct cgtggtggcg gccgcctcgg cg
//
LOCUS       DASUPW010000289         2733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1453495, whole genome shotgun sequence.
ACCESSION   DASUPW010000289 DASUPW010000000
VERSION     DASUPW010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453495"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VFY44_06560"
     CDS             <1..464
                     /locus_tag="VFY44_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910138.1"
                     /translation="ERRLTAESDSAPLVVLDASDPPSEEPLDAAVRAAASLSVFVARR
                     GGMALLLPGDRRPTVLPADLAGWPSLHARLALVGPAAQRPPLARARRSGAVIWVSARP
                     DPPRDLARSTAGAGWLVTPLEDQDGDAAFEVAGCGGRRITRKAAAGSGVAA"
     gene            461..2707
                     /locus_tag="VFY44_06565"
     CDS             461..2707
                     /locus_tag="VFY44_06565"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HEX5910139.1"
                     /translation="MSERRTAGLLQLALFAALGSFAALHWAALVESPPVGRILLAVAA
                     LAAVGALLQVLNRSRLPRPVVHAAALVAVLVGAAAALAVTGLSVRLLWPGNWDELGNQ
                     LEQGLSGVQTVVWPYGGPEDDVRLAILLGAPALLALAAGLAFWPVRSRITASVLRFMG
                     LVALLCLYGIPVTDHDPGEPLARGLVLFALVAAWLWAPRMRGREAVPALVAVAVVGLV
                     ALPLAARVDRAEALVDYSDWQWFGGKEISFDWDHSYGPLDWPRDGTTLLQVRSPRPLY
                     WKAEVLDTFDGLRWVRSRSNDRTSPLGELPDDPDTRWDRSITVTVRSLRTDFVIGTGT
                     PYRVLGAGEAVSGAADGTLRRLSEPLRRGDEYTVRAYAPTPSARQMREATGSYDIELS
                     QYTDVVLPRQGENALDTADRAGSVAARERIEVPLRGGDAPAADPGALRFAGSPYARTE
                     RLARRLTADAPTAYDAARAVQDHFRSAEFSYSERPPSRRYPLDAFLFDDKIGYCQQFS
                     GAMALMLRMSGIPARVATGFSPGSLNRDTGEYRIRDLDAHSWVEVYFSGIGWVTFDPT
                     PPAAPADRAGQGPRAAPEGEDRPRDALSGDGDSPLSDRAADTGAASRSGGGGQGGGPP
                     AGVLVGIALAALAGGAGVLLTRRRLRPHGTAEAGLAELERALPRLGWKLPSGTTLLQL
                     EQRLLRAAGPASARYVRQLRTLRFGAGQGTPPLGGDRRAMRRELSAGRGPLARLRGFV
                     ALPPRRAG"
BASE COUNT          262 a         1051 c         1066 g          354 t
ORIGIN      
        1 tggagcgccg cctgaccgcc gagtccgact cggcgccgct ggtggtgctg gacgccagcg
       61 atccgccgtc ggaggagccg ctggacgccg cggtgcgcgc cgccgcctcg ctgtcggtgt
      121 tcgtcgcccg ccgtgggggg atggcgctgc tgctgccggg cgaccggcgc ccgaccgtgc
      181 tgcccgccga cctggccgga tggccgtccc tgcacgcccg cctcgccctg gtcggcccgg
      241 ccgcccaacg cccgccgctg gcgcgtgccc gccgctcggg cgccgtgatc tgggtcagcg
      301 cgcggcccga cccgcctcgc gacctggcgc ggtccaccgc cggcgcgggg tggctggtca
      361 ccccgctcga ggaccaggac ggcgacgccg ccttcgaggt cgccggatgc ggcggccgcc
      421 ggatcacccg caaggcggcg gcgggctcgg gggtggccgc gtgagcgagc gccggaccgc
      481 cggcctcctc cagcttgccc tgttcgcggc cctgggctcc ttcgccgcgc tgcactgggc
      541 ggcgctggtg gagagcccgc cggtggggcg gatcctgctg gccgtggccg cgctggccgc
      601 ggtgggggcc ctgctccagg tgctcaaccg ctcgcgcctg ccccgcccgg tggtgcacgc
      661 ggccgcgctg gtggcggtcc tcgtgggcgc ggccgccgcg ctggcggtca ccggcctgtc
      721 cgtgcggctg ctctggccgg gcaactggga cgagctgggc aaccagctcg agcagggcct
      781 gtcgggcgtc cagacggtgg tgtggcccta cggcgggccc gaggacgacg tccgcctggc
      841 catcctgctc ggcgccccgg cgctgctggc gctggccgcc gggctggcct tctggcccgt
      901 gcgctcgcgg atcaccgctt cggtgctgcg cttcatgggc ctggtggcgc tgctgtgcct
      961 atacggcatc ccggtcaccg accacgaccc cggagagccg ctggcgcgcg gcctggtgct
     1021 gttcgcgctc gtcgcggcct ggctgtgggc gccgcggatg cgcggccgtg aggcggtgcc
     1081 cgccctggtc gcggtcgcgg tggtggggct ggttgcgctc ccgctggcgg cccgcgtgga
     1141 ccgcgccgag gcgctggtgg actacagcga ctggcagtgg ttcgggggca aggagatcag
     1201 cttcgactgg gaccattcgt acgggccgct ggactggccc cgcgacggca ccacgctgct
     1261 ccaggtcagg tcgccgcggc cgctgtactg gaaggccgag gtgctggaca ccttcgacgg
     1321 gctgcgctgg gtgcgctcgc gctcgaacga ccggaccagc ccgctgggcg agctccccga
     1381 cgaccccgac acgcgctggg accgcagcat cacggtcacg gtccgctcgc tgcgcacgga
     1441 cttcgtgatc gggaccggca cgccgtaccg cgtgctgggg gcgggggagg ccgtcagcgg
     1501 cgcggccgac ggcaccctcc ggcggctgtc cgagccgctg cggcggggcg acgagtacac
     1561 ggtccgcgcc tacgcgccca cgccgtcggc ccgccagatg cgcgaggcga ccggctccta
     1621 cgacatcgag ctctcccagt acaccgacgt cgtgctcccg cggcagggcg agaacgcgct
     1681 ggacacggcc gaccgggccg gttcggtggc ggcgcgcgag cggatcgagg tcccgctgcg
     1741 cggtggcgat gcgcccgcgg ccgaccccgg tgccctgcgc ttcgccgggt cgccctacgc
     1801 ccgcaccgag cgcctggcgc gccggctgac cgccgacgcc ccgacggcgt acgacgccgc
     1861 ccgcgcggtc caggaccact tccgctccgc cgagttcagc tacagcgagc gcccacccag
     1921 ccgtcgctac ccgctggacg ccttcctgtt cgacgacaag atcggctact gccagcagtt
     1981 ctcgggcgcg atggcgctga tgctgcgcat gtccggcatc cccgcccggg tggccaccgg
     2041 gttctcgccc ggatcgctga accgcgacac cggtgagtac cggatccggg acctggacgc
     2101 gcactcctgg gtcgaggtct acttcagcgg catcggctgg gtcacgttcg accccacgcc
     2161 gcccgcggcg ccggccgacc gcgcgggcca gggtccgcgc gccgcccccg agggcgagga
     2221 ccgcccgcgc gacgcgctgt cgggcgacgg cgactcgccg ctgtccgacc gcgcggccga
     2281 cacgggcgcg gcctcccgtt ccggcggggg cgggcagggc ggcggcccgc ccgccggcgt
     2341 gctggtaggg atcgcgctcg ccgcgctggc gggtggcgcg ggcgtgctgc tcaccaggcg
     2401 ccgcctgcgc ccgcacggga ccgcggaggc cgggctggcc gagctggagc gggcgctgcc
     2461 gcggctgggg tggaagctgc cgtcggggac caccctgctc cagctcgagc agcggctgct
     2521 cagggccgcg gggcccgcct cggcgcggta cgtgcgccag ctgcgcacgc tgcgcttcgg
     2581 cgccggccag ggcacgccgc cgctgggggg cgaccgccgc gcgatgcgcc gcgagctgag
     2641 cgcgggccgc ggtccgctgg cgcgcctgcg aggattcgtg gccttgccgc cccgtcgggc
     2701 cggatagggc aaggatgcac tatccggcgc atc
//
LOCUS       DASUPW010000290         2039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_622596, whole genome shotgun sequence.
ACCESSION   DASUPW010000290 DASUPW010000000
VERSION     DASUPW010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622596"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VFY44_06570"
     CDS             <1..372
                     /locus_tag="VFY44_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910140.1"
                     /translation="YLLRERPSVGPFRNVKVGTRHIHHFVPGIALAFASGGAAIVTRN
                     DTVERWMALPFGVGMGLTLDESALLLELDDVYWSEEGILSVQITLAVTALLAATALAA
                     RVLMRGEQEVLPGLPPDAPGL"
     gene            776..1279
                     /locus_tag="VFY44_06575"
     CDS             776..1279
                     /locus_tag="VFY44_06575"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX5910141.1"
                     /translation="MPPGGWQQPAAATPALPGELASWGSRVGAQLLDALIVTVAVILM
                     IVVIVFAFALSDVLGIILAVLLGLAIVALYVGYGGYFMARDGSRNGQTLGKQIVGIRA
                     VRDNGQPFDLWTGILREFVVKNLLIGFVGGFFFSIPTILNYLWPLWDDSDRCLHDMIV
                     STHVVRN"
     gene            1291..1743
                     /locus_tag="VFY44_06580"
     CDS             1291..1743
                     /locus_tag="VFY44_06580"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX5910142.1"
                     /translation="MELASWWSRVGAWLLDALIRVLLAGAAFGLLIAVDSVGGVVDTV
                     GTIVLAPAVVIVYFGYPALTMRRPGARNGQTWGKQIVGIRAVRDDGAAYGLGTALVRE
                     LLIKELLIGFVGAIAFSIPFLLDSLWPLWDETNRAVHDMIAHSHVVRA"
     gene            complement(1753..>2039)
                     /locus_tag="VFY44_06585"
     CDS             complement(1753..>2039)
                     /locus_tag="VFY44_06585"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HEX5910143.1"
                     /translation="YSGPEGASFEEFPYHQSVLHHVRGETVELPGWHEDITGARTLED
                     LPENARAYIRYIEEQLGVPVVTVGVGPGREQIIWTEAAASVRRQSAADAA"
BASE COUNT          253 a          742 c          746 g          298 t
ORIGIN      
        1 tacctgctgc gcgagcgccc gtcggtcggg cccttccgca acgtgaaggt cggcacgcgc
       61 cacatccacc acttcgtgcc cgggatcgcg ctggcgttcg cctccggcgg ggcggccatc
      121 gtgacccgca acgacacggt cgagcgctgg atggcgctcc ccttcggcgt cggcatgggg
      181 ctgacgctgg acgagtccgc gctgctcctg gagctggacg acgtgtactg gtccgaggag
      241 ggcatcctga gcgtccagat caccctcgcc gtcaccgcgc tgctggccgc caccgccctg
      301 gccgcgcggg tgctcatgcg cggtgagcag gaggtgctcc ccggcctgcc acccgacgcg
      361 ccgggactgt agggtgccgc cgatggaggg atcaggaggc caggggccct tcgggccagg
      421 ctcccccggc tacgggccgg gaggggcggg aaacgacgac gaggaccgcg acgccgagcg
      481 ctccggcacg ggcccggagg cgccgcagcg cccgcccgcc gaggagcctc cggccccgct
      541 ctggtcggcg cccaagaacg cagagccgcc gcccccggcg ggaggaccgc agagccccgc
      601 tcccggtggg ccccagagtc ccgctcccgc aggcccccag agccccgctc ccggtgggcc
      661 ccagagcccc gcgccgggag ggccccagag cccggccccg ggtgccgggc tccaggcgcc
      721 gccaccgccc ccgggcgggc cccaggcgcc cgcggctccg ggatacggcg gccccgtgcc
      781 gcccggcggc tggcagcagc ccgccgcggc cacgccggcg ctcccgggtg agctggccag
      841 ctggggcagc cgagtgggcg cgcagctgct cgatgccctg atcgtgaccg tggcggtcat
      901 cctgatgatc gtggtcatcg tcttcgcctt cgcgttgagc gacgtgctcg ggatcatcct
      961 cgccgtgctc ctcggcctgg ccatcgtggc gctctacgtc ggctacgggg ggtacttcat
     1021 ggctcgcgac ggaagccgca acgggcagac cctggggaaa cagatcgtgg gcattcgcgc
     1081 cgtgcgcgac aacggccagc cgttcgacct gtggaccggc atcctgcggg agttcgtggt
     1141 caagaacctg ctgatcgggt tcgtgggcgg gttcttcttc tcgatcccca ccatcctcaa
     1201 ctacctctgg ccgctgtggg atgacagcga ccggtgtctg cacgacatga tcgtgtcgac
     1261 gcacgtcgtc cgcaactgac cgaaccggcc gtggagctgg ccagctggtg gagccgcgtc
     1321 ggcgcctggt tgctcgacgc cctgatccgg gtgctcctgg ccggcgcggc gttcggcctg
     1381 ctgatcgcgg tcgactcggt gggcggggtg gtggacaccg tggggacgat cgtcctcgca
     1441 cccgccgtgg tgatcgtgta cttcggctac cccgcgctga cgatgcggcg ccccggcgcc
     1501 cgcaacggcc agacctgggg caagcagatc gtgggcatca gggccgttcg cgacgatggc
     1561 gcggcctacg gtctcggcac cgcgctggtg cgcgagctcc tgatcaagga gctgctgatc
     1621 gggttcgtgg gcgccatcgc cttctcgatc ccgttcctcc tggactcgct gtggccgctg
     1681 tgggacgaga cgaaccgcgc ggtgcacgac atgatcgccc actcgcacgt cgtgcgggcc
     1741 tgagcggggg gctcaggccg cgtcggcagc cgactggcgc cgcaccgacg cggcagcctc
     1801 ggtccaaatg atctgctcgc gcccggggcc cacgccgacg gtgaccacgg ggacgcccag
     1861 ctgctcctcg atgtagcgga tgtaggcgcg cgcgttctcc ggcaggtcct cgagggtgcg
     1921 ggcgccggtg atgtcctcgt gccagccggg cagctccacg gtctcgccgc ggacgtggtg
     1981 gagcacggac tggtggtagg ggaactcctc gaagctggcg ccctctggcc cggagtacg
//
LOCUS       DASUPW010000291         2997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_899986, whole genome shotgun sequence.
ACCESSION   DASUPW010000291 DASUPW010000000
VERSION     DASUPW010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899986"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1634
                     /locus_tag="VFY44_06590"
     CDS             <1..1634
                     /locus_tag="VFY44_06590"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5910144.1"
                     /translation="LPRVRPGHGRPALMRRLLTAVAVIALVAATVVLVGGGGDGARKT
                     YKIEFDSGFGLVEGGDLKIGGVKAGKTTAFALERKEPYRVVVTAQVTEPGFDSLRSDA
                     RCEVRAQSLIGEYFVDCEAGRAEREIPDGGRVPVARTASTIPPDLVNTVMRRPYKERF
                     RLILSELGTGLAGRPEDLNEVIRRAHPALRETSQTIAILRGQNTVIRDFIRDADQVSL
                     KVDPVREQVAKWAREAEDVTSVQASRSEELGRYYNRLPRLLAELRPTMAELERTADAQ
                     IPTLRKLRGAAPELTAFLRDAEPFARASRNSIDDLGRAAGAGRAAIRQSSQEVGQLRR
                     VAAFAPRLGKPLRQFLQSIDDRKRSTERDPLARTTAPPAPDKTAYKEGQGFTGMEGLL
                     NYVYYQTLGINGFDEFGHLLRITAFTAGPCSPYSTNPTEAVRRQCASHLGPYQPGVTS
                     PDPTVNGTAAKERAQAAKPVEAAERTPRKPGEPEAPPVKGQRDISKPQVVLPDNVRQL
                     LDQLRKPVEGVPQPALPQTPGGSTDIDVLDYLLSP"
     gene            1631..>2997
                     /locus_tag="VFY44_06595"
     CDS             1631..>2997
                     /locus_tag="VFY44_06595"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5910145.1"
                     /translation="MRRRTTQSITASPVLVGAVTVLVALVAVYLAYNANAGLPFVPTY
                     DVRAQIPGGSNLVEANEVRIGGFRAGAVDRIRPGIARPGNRAQGQDRDARSVAIIEMK
                     LDKRFEPIARDSRIQIRPRSALGLKYVDLVPGKSAQKLKPGGTLPLAQSLKPVELDDF
                     FSTYDDDFRVNQRTVLEGYGNALAGRGQDINTVIEGLVPFVRKLEPVMRTLSDDRTEL
                     NNFFRQAGRTSAQIAPVADTYADLFVNMGTTFEALSADETKLRETIERLAPTAEEGIR
                     SFPVQRPFLRDSAELARRLEPVAEQIEVSLPVTGSALRTGTPVLKKAPPFYRRTREVF
                     RSLDELTANPNTLLALRDLHSTVQVTTPLLEHVAPYQTVCNYWNYYWTAISEHVSEEV
                     RGGTTQRTNLKSDNRTQDNRLSDTSADRPVDTPAGQDPITGRAPTGDPWQALHRPAYQ
                     PAIDA"
BASE COUNT          468 a         1122 c         1043 g          364 t
ORIGIN      
        1 gactgccgcg agtccgaccg ggccacggga gacctgccct gatgcgccgg ctgctcaccg
       61 ccgtcgcggt catcgccctc gtcgctgcca cggtggtgct ggtgggcggt ggcggtgacg
      121 gcgcgcgcaa gacctacaag atcgagttcg acagcggctt cgggctggtc gagggcggcg
      181 acctgaagat cggcggcgtc aaggccggca agaccaccgc gttcgcgctc gagcgcaagg
      241 agccctaccg cgtggtggtc acggcgcagg tgaccgagcc gggcttcgac tcgctgcgca
      301 gcgatgcccg ctgcgaggtg cgggcccagt cgctgatcgg cgagtacttc gtggactgcg
      361 aggccgggcg ggccgagcgc gagattcccg acggcggccg cgtgccggtc gcgcggacgg
      421 cgtcgaccat cccgcccgac ctggtgaaca cggtcatgcg gcgcccgtac aaggagcgct
      481 tccgcctgat cctgtccgag ctgggcaccg gcctcgcggg gcgcccggag gatctgaacg
      541 aggtcatccg ccgcgcccat ccggcgctgc gcgagaccag ccagacgatc gcgatcctgc
      601 gcggccagaa cacggtcatc cgcgacttca tccgtgacgc cgaccaggtc tcgctgaagg
      661 tggacccggt cagggagcag gtggccaagt gggcccgcga ggccgaggac gtgaccagcg
      721 tccaggcctc gcgcagcgag gagctgggcc gctactacaa ccgcctgccg cgcctgctgg
      781 ccgagctgcg gcccacgatg gccgagctgg agcgcaccgc cgacgcccag atccccaccc
      841 tgcgcaagct gcgcggcgcg gcgcccgagc tgaccgcgtt cctgcgtgac gccgagccgt
      901 tcgcgcgcgc ctcgcgcaac tcgatcgacg acctgggccg ggccgcgggc gccggtcgcg
      961 ccgccatccg ccagtcctcg caggaggtgg gccagctacg ccgggtcgcg gccttcgcgc
     1021 cgcgcctggg caagccgctg cgccagttcc tgcagtcgat cgacgaccgc aagcgctcga
     1081 ccgagcgcga cccgctggcc cggacgacgg cgccgcccgc tcccgacaag accgcctaca
     1141 aggaggggca ggggttcacc ggcatggagg gcctcctgaa ctacgtctac taccagacgc
     1201 tgggcatcaa cggattcgac gagttcggcc acctgctgcg gatcacggcg ttcaccgccg
     1261 ggccctgctc gccgtactcg accaacccca ccgaggccgt gcgcaggcag tgcgcctcgc
     1321 acctcggccc ctaccagccg ggggtgacca gccccgaccc gaccgtgaac ggcacggccg
     1381 ccaaggagcg cgcccaggcc gcgaagccgg tcgaggcggc cgagcgcacg ccccgcaagc
     1441 cgggcgagcc cgaggccccg cccgtgaagg gacagcgcga catctccaag ccccaggtcg
     1501 tcctgcccga caacgtcagg cagctgctcg accagctgcg caagccggtg gagggagtgc
     1561 cgcagccggc gctgccgcag accccgggcg gctccaccga catcgacgtc ctcgactacc
     1621 tgctgagccc atgagacgcc ggacgacaca gtcgatcacc gccagccccg tgctggtggg
     1681 cgccgtgacg gtgctggtgg cgctggtggc cgtgtacctg gcctacaacg ccaacgcggg
     1741 cctgcccttc gtgcccacct acgacgtccg ggcgcagatc ccgggcggct ccaacctggt
     1801 ggaggcgaac gaggtccgga tcggcggctt ccgcgccggc gccgtggacc ggatccgtcc
     1861 cggcatcgcc cggccgggca accgggcgca gggccaggac cgcgacgcgc gctcggtggc
     1921 gatcatcgag atgaagctgg acaagcgctt cgagccgatc gcgcgcgact cgcggatcca
     1981 gatccgcccg cgctcggcgc tggggctgaa gtacgtggac ctggtgcccg ggaagtcggc
     2041 ccagaagctc aagcccggcg ggacgttgcc gctggcccag tcgctcaagc cggtggagct
     2101 ggacgacttc ttcagcacct acgacgacga cttccgcgtg aaccagcgca cggttctgga
     2161 gggctacggc aacgcgctgg ccggccgcgg ccaggacatc aacaccgtga tcgagggcct
     2221 ggtgccgttc gtgcgcaagc tcgagccggt catgcggacg ctgtcggacg accgcaccga
     2281 gctgaacaac ttcttccgcc aggccgggcg cacgtcggcc cagatcgcgc cggtggccga
     2341 cacctacgcc gacctgttcg tgaacatggg caccacgttc gaggcgctct cggccgacga
     2401 gacgaagctg cgcgagacca tcgagcggct cgcgcccacc gccgaggagg gcatccgcag
     2461 cttcccggta cagcgcccgt tcctgcgcga ctcggccgag ctggcacggc ggctggagcc
     2521 ggtggccgag cagatcgagg tgtccctgcc cgtgacgggc tcggccctgc ggacgggcac
     2581 gccggtgctg aagaaggccc ctccgttcta ccgccgcacg cgcgaggtgt tccgctcgct
     2641 ggacgagctg acggccaacc cgaacacgct gctggcgctg cgcgacctgc acagcacggt
     2701 ccaggtgacc acgccgctgc tggagcacgt ggcgccgtac cagacggtct gcaactactg
     2761 gaactactac tggaccgcga tctccgagca cgtgtccgag gaggtgcgcg gcgggaccac
     2821 gcagcgcacc aacctcaagt ccgacaaccg cacccaggac aaccgcctgt ccgacaccag
     2881 cgccgaccgc ccggtggaca cgcccgccgg ccaggacccg atcaccggcc gcgcccccac
     2941 cggcgatccg tggcaggcgc tgcaccggcc cgcctaccag ccggcgatcg acgcgca
//
LOCUS       DASUPW010000292         5873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_347399, whole genome shotgun sequence.
ACCESSION   DASUPW010000292 DASUPW010000000
VERSION     DASUPW010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347399"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..586)
                     /locus_tag="VFY44_06600"
     CDS             complement(170..586)
                     /locus_tag="VFY44_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX5910146.1"
                     /translation="MGPDEIREFLTHGTRTGKLAVLTPSGHPHVMPIWFVLDGDDIVF
                     NTGAKTVKGKHILADGRVSLAVDDEQPPFAYVHIRGTATARDNPPDMLEWATRIGARY
                     MGEDRAEEFGRRNGVPGELLVRLTPERVIAERGVAD"
     gene            complement(597..1592)
                     /gene="moaA"
                     /locus_tag="VFY44_06605"
     CDS             complement(597..1592)
                     /gene="moaA"
                     /locus_tag="VFY44_06605"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX5910147.1"
                     /translation="MSREPLRDGHGRLIQDLRVSVTDRCNFRCQYCMPAEGLPWLDRD
                     SVLTFEEIERLVSLLAQMGVTDLRLTGGEPLVRKDFPVLAGMLAAVPGLQDLSVTTNG
                     YLLARDAEALAAAGVKRLNVSIDSLQRDRFFEMTRRDSLPQVLEGLEAAAAQPSLRPI
                     KINAVALRGFTEQEALPFAEFARERDFEVRFIEFMPLDGDQAWTADQVLTGDELREII
                     HAVHPLEEVEREPSATARVYRFADGAGQIGFINPVSEPFCSDCNRIRLTAEGKLRTCL
                     FSMNETDLREPLRSGASDDELERIVRDAVWRKELKHRVGEKGFRQPARSMSAIGG"
     gene            1647..3425
                     /locus_tag="VFY44_06610"
     CDS             1647..3425
                     /locus_tag="VFY44_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX5910148.1"
                     /translation="MSQNLIEMLRAREGDTPYRGTGGHPTALAALKEAMARDGEITDD
                     ARRHAAEVSGLPEATVYGISTFYDDLVEPRGRRHVAVCTGTACWASHPGDHAAELGER
                     LGVELGERSEDGSVSYGATVCLGFCHSSPAYRDGTVVDAGPGALERVASGGAEQAPEP
                     DGRTMLDEPVLLGTGTFAGLRRALAELSPEELLEEVTEANLRGRGGAGFPAGKKWSFA
                     RDAEGDEKLIVVNGDEGDPGSYIDKHLMEQCPRLLLEGMALAGYAVGSEHGLVLVRSE
                     YPDSTPVLREAIEQAYAEGELGDDIHGSGFSFHVQVEEGAGSYVVGEETALLGSLQGL
                     RGTVNARPPFPAQRGWHGKPTVVNNVETLCNAPYIAANGAEAYKALSPDATPGVKLVC
                     LNERFVNPGIFEVPFGTPVSHICNELGGGLKDGKQIKAVQIGGPLGGVLPGRLLDTPL
                     DFDDLAEHGCMVGHGSILAFDQDTDIRDLCTHLLEFGADESCGKCFPCRIGLQRAVEM
                     SEAPGDIDREKLEALLETLELGSLCAHGGGMPAPIRTLIEFFPDELGLTAEKGPDKAE
                     QEKESEGDRRPEEEATTDDPQEPSGA"
     gene            3422..4048
                     /locus_tag="VFY44_06615"
     CDS             3422..4048
                     /locus_tag="VFY44_06615"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX5910149.1"
                     /translation="MKVTVDGIDVDVPDGATVLEAARAAGRWVPTLCFDERMDPFGAC
                     RVCMVGLEGQDGPIAACTTPCSDGMEVHTTDATARRVATATVELVLSELPSMPGDHTE
                     LMEVAERLQVGEPRWTGAVHEPRHDERHPYLSFHHELCISCGRCVRACDEIQGAFALT
                     ATGRGFESNVAAGLNSGFTDSTCVSCGACADTCPTDAINEHSLLDLLA"
     gene            4045..>5873
                     /gene="fdhF"
                     /locus_tag="VFY44_06620"
     CDS             4045..>5873
                     /gene="fdhF"
                     /locus_tag="VFY44_06620"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEX5910150.1"
                     /translation="MSGRFEQTTTTTCGYCGVGCRLEAHRVDGAVRSIAPAMDGPANK
                     GHTCVKGRFAHQFARSRDRLTAPLVRENEGFRLASWEEAIAKIVDAFTSIKAEHGPDA
                     IAGLASSRATNEDCYVMQRFMRATVGTHNIDNCSRVCHSPTSFALRKSLGLSGATGSF
                     DDFERADVALLVGVNPTQGHPVVGARIKQAALKGLKLITADPRRIELSDYGQIHLPMR
                     PGTNAALLNGLCHVVIRDGLTDQEFIRERTDGYDAVAELVEAYTPESVEEITGIPAAD
                     IVAAARMYAGADRACISWGLGVTEHLYGSEVVQLICNLALLTGNVGKPGAALLPLRGQ
                     NNVQGSSDHGALPDTYTDYMPVTDDDVTSRFEKRWGVSMKREPGMKIPEMFDAAVEGS
                     LKAMYIFGEDVAQTDPDTAHVEKALGALDFLVCQDIFETETTKFADVILPASAFLEKT
                     GTFTNAERRLQMVEPGAAPPGSARTDFDIITSGSKALGYDMGSETPSDVMAEVAEMTP
                     RYAGISHERIGRDGLQWPVAPDGTDTPILYEEHFETGDGLGHFAALPYKPPGDAADDE
                     FPFILVTGRRLEHYNAGTMTRRTGNLTLMDHDWLEVHPDDAAKL"
BASE COUNT          879 a         2090 c         2111 g          793 t
ORIGIN      
        1 ggtcagcagc tcctgggcca tggccccgct cccgccgccg atctccagca cgtcgggccc
       61 cagccggtcg ccctcctcca gcacccaggg caggacgacc ctgcgcgcga agccacgcca
      121 gggagccgag cggcagaaca cggcctcgac gctggacatg gcgcccacgt cagtccgcca
      181 cgccgcgctc ggcgatcacc cgctcgggcg tgagccggac cagcagctcg ccaggcacgc
      241 cgttgcggcg gccgaactcc tcggcgcggt cctcgcccat gtaccgggcc ccgatccggg
      301 tcgcccactc gagcatgtcc ggcgggttgt cccgggcggt cgcggtgccg cggatgtgca
      361 cgtaggcgaa cggcggctgc tcgtcgtcca ccgccagcga cacgcgcccg tcggccagga
      421 tgtgcttgcc cttcacggtc ttcgcacccg tgttgaagac gatgtcgtcg ccatccagca
      481 cgaaccagat cggcatcacg tgcgggtggc cgctgggggt gaggacggcc agcttgccgg
      541 tgcgcgtgcc gtgggtcagg aactcccgga tctcgtccgg gcccatctcg gccacgtcag
      601 ccgccgatgg cgctcatgct gcgggcgggc tggcggaaac ccttctcgcc gacgcggtgc
      661 ttgagctcct tgcgccacac ggcgtcgcgg acgatccgct ccagctcgtc gtccgaggcg
      721 cccgagcgca gcggctcgcg caggtcggtc tcgttcatcg agaacaggca cgtgcgcagc
      781 ttgccctccg cggtcaggcg gatgcggttg cagtccgagc agaacggctc ggacaccggg
      841 ttgatgaagc cgatctggcc ggcgccgtcg gcgaagcggt agacccgcgc ggtggccgac
      901 ggctcgcgct cgacctcctc cagcgggtgc acggcgtgga tgatctcccg cagctcgtcg
      961 ccggtgagca cctggtcggc ggtccaggcc tggtcgccgt ccagcggcat gaactcgatg
     1021 aaccgcacct cgaagtcgcg ctcgcgggcg aactcggcga acggcagcgc ctcctgctcg
     1081 gtgaacccgc gcagcgccac ggcgttgatc ttgatcgggc gcagcgaggg ctgggccgcg
     1141 gcggcctcga gcccctccag cacctggggc agcgagtcgc gccgggtcat ctcgaagaag
     1201 cggtcgcgct ggagcgagtc gatcgagacg ttcaggcgct tgaccccggc cgcggccagc
     1261 gcctcggcgt cgcgggccag caggtagccg ttcgtggtca ccgacaggtc ctgtaggccg
     1321 ggcaccgcgg ccagcatccc ggccagcacg gggaagtcct tgcgcaccag cggctcgccc
     1381 ccggtcaggc gcaggtcggt cacgcccatc tgggccagca gcgacaccag ccgctcgatc
     1441 tcctcgaacg tgagcacgga gtcgcggtcc agccacggca ggccctccgc gggcatgcag
     1501 tactggcagc ggaagttgca gcggtcggtg accgagaccc ggagatcctg gatcagccgc
     1561 ccgtgtccat ccctcagtgg ctcacgactc atgcacggac aacgctagcg ctgtgccgcg
     1621 ccgggtacgt tgcgcgtgag gagcagatgt cgcagaacct gatcgagatg cttcgcgccc
     1681 gcgagggcga taccccatat cggggcacag gcggccaccc aacggcactc gccgcgctca
     1741 aggaggccat ggcgcgcgac ggcgagatca ccgacgacgc gcgccggcat gcggccgagg
     1801 tgtctggcct tcccgaggcc acggtgtacg ggatctccac cttctacgac gacctggtgg
     1861 agccgcgcgg ccggcgccac gtggccgtgt gcaccggcac cgcctgctgg gcgtcgcacc
     1921 cgggcgatca cgcggccgag ctcggcgagc ggctcggggt cgagctcggc gagcggtccg
     1981 aggacggctc ggtgtcctac ggcgcgaccg tgtgcctggg cttctgccat tcgtcgccgg
     2041 cgtaccgcga cggcacggtg gtggacgccg gtccgggcgc gctggagcgg gtggcctcgg
     2101 gcggcgccga gcaggcgccc gagcccgacg ggcggaccat gctcgacgag ccggtcctgc
     2161 tcggcacggg caccttcgcc gggctgcgcc gggcgctggc cgagctgtcg cccgaggagc
     2221 tgctggagga ggtcacggag gccaacctgc gcggccgcgg cggggcgggc ttcccggcgg
     2281 gcaagaagtg gtcgttcgcg cgcgacgcgg agggcgacga gaagctgatc gtggtcaacg
     2341 gcgacgaggg cgaccccggc tcgtacatcg acaagcacct gatggagcag tgcccgcggc
     2401 tgctgctgga gggcatggcg ctggccgggt acgcggtcgg ctccgagcac ggcctggtcc
     2461 tggtgcgctc ggagtacccg gactccacgc cggtgctgcg cgaggcgatc gagcaggcgt
     2521 acgccgaggg cgagcttggc gacgacatcc acggcagcgg cttctccttc cacgtgcagg
     2581 tcgaggaggg cgccggctcc tacgtggtcg gcgaggagac cgcgttgctc ggttcgctcc
     2641 agggcctgcg cggcacggtg aacgcgcggc cgccgttccc cgcgcagcgc ggatggcacg
     2701 gcaagcccac ggtggtcaac aacgtcgaga cgctgtgcaa cgcgccctac atcgccgcca
     2761 acggggccga ggcctacaag gcgctgtcac cggacgcgac gccgggcgtg aagctggtgt
     2821 gcctgaacga gcggttcgtg aacccgggca tcttcgaggt gccgttcggc acccccgtct
     2881 cgcacatctg caacgagctg ggcggcgggc tcaaggacgg caagcagatc aaggcggtgc
     2941 agatcggcgg cccgctgggg ggtgtgctgc ccggccggct gctggacaca ccgctggact
     3001 tcgacgacct ggccgagcac ggctgcatgg tcggccacgg ctcgatcctg gcgttcgacc
     3061 aggacaccga catccgcgat ctgtgcacgc acctgctgga gttcggcgcc gacgagagct
     3121 gtggcaagtg cttcccgtgc cggatcggcc tgcagcgcgc ggtcgagatg tccgaggcgc
     3181 ccggcgacat cgaccgcgag aagctcgagg cgctgctgga gacgctggag ctgggatcgc
     3241 tgtgtgccca tggcggcggc atgccggcgc ccatccggac gctgatcgag ttcttccccg
     3301 acgagctggg gctgacggcc gagaagggcc cggacaaggc cgagcaggag aaggagtccg
     3361 agggtgaccg ccgccccgag gaggaggcca ccaccgacga cccccaggag ccgagcggcg
     3421 catgaaggtc acggtcgacg gcatcgacgt ggacgtgccc gacggcgcca ccgtgctcga
     3481 ggccgcgcgc gccgccggcc ggtgggtgcc cacgctgtgc ttcgacgagc gcatggaccc
     3541 gttcggagcc tgccgcgtgt gcatggtcgg gctcgagggc caggacggcc cgatcgccgc
     3601 ctgcaccact ccgtgctcgg acgggatgga ggtgcacacc accgacgcca cggcgcggcg
     3661 ggtggccacg gccaccgtgg agctggtcct gagcgagctg ccctctatgc cgggtgacca
     3721 caccgagctg atggaggtcg ccgagcggct gcaggtcggc gagccgcgct ggaccggtgc
     3781 ggtgcacgag ccccgccacg acgagcgcca cccgtacctg tccttccacc acgagctgtg
     3841 catctcgtgc gggcgttgcg tgcgcgcgtg tgacgagatc cagggtgcgt tcgcgctgac
     3901 cgccaccggc cgggggttcg agtccaacgt ggccgccggg ctgaactccg gcttcaccga
     3961 ctccacgtgc gtgtcgtgcg gagcgtgcgc cgacacgtgc cccacggacg ccatcaacga
     4021 gcattcgctg ctagacctac tggcatgagt gggcggttcg aacagaccac cacgaccacc
     4081 tgcggctact gcggagtcgg ctgccggctg gaggcgcacc gggtggacgg cgccgtgcgc
     4141 tcgatcgcgc ccgccatgga cggcccggcg aacaagggcc acacctgcgt caagggtcgc
     4201 ttcgcccacc agttcgcgcg ctcgcgcgac cgcctcacgg cgccgctggt gcgcgagaac
     4261 gaggggttcc ggctggcctc gtgggaggag gcgatcgcga agatcgtgga cgcgttcacc
     4321 tcgatcaagg ccgaacacgg gcccgacgcc atcgccggcc tggcctcctc gcgcgcgacg
     4381 aacgaggact gctacgtgat gcagcgcttc atgcgcgcca ccgtgggcac gcacaacatc
     4441 gacaactgct cgcgcgtgtg ccactcgccg acctcgttcg ctctgcgcaa gtcgctgggc
     4501 ctgtcgggcg ccaccggctc gttcgacgac ttcgagcgcg ccgacgtggc gctgctggtg
     4561 ggggtgaacc cgacgcaggg ccacccggtg gtgggggcgc ggatcaagca ggccgcgctg
     4621 aaggggctga agctgatcac cgccgacccg cgccggatcg agctgtccga ctacggccag
     4681 atccacctgc cgatgcgccc cgggaccaac gccgcgctgc tcaacgggct ctgccacgtg
     4741 gtcatccgcg atgggctcac cgaccaggag ttcatccgcg agcgcaccga cgggtacgac
     4801 gccgtggccg agctcgtgga ggcctacacg ccggagtcgg tcgaggagat caccgggatc
     4861 cccgccgccg acatcgtcgc cgccgcccgc atgtacgccg gcgccgaccg cgcctgcatc
     4921 tcgtggggcc tcggcgtgac cgagcacctg tacggctccg aggtggtgca gctgatctgc
     4981 aacctcgcgc tgctgaccgg caacgtcggc aagccgggcg ccgcgctgtt gccgctgcgg
     5041 ggccagaaca acgtgcaggg ctcctccgac cacggcgcgc tgccggacac ctacaccgac
     5101 tacatgccgg tcaccgacga cgacgtcacg tcgcgcttcg agaagcgctg gggcgtgtcg
     5161 atgaagcgcg agcccggcat gaagatcccc gagatgttcg acgccgcggt cgaggggtcg
     5221 ctgaaggcga tgtacatctt cggcgaggac gtggcccaga ccgacccgga caccgcccac
     5281 gtggagaagg cgctgggcgc gctggacttc ctggtctgcc aggacatctt cgagaccgag
     5341 accaccaagt tcgccgacgt gatcctgccc gcctcggcct tcctggagaa gacgggcacg
     5401 ttcaccaacg cggagcggcg gctgcagatg gtcgagcccg gggcggcgcc ccccgggagc
     5461 gcccgcaccg acttcgacat catcacctcg ggctcgaagg ccctgggcta cgacatgggc
     5521 tccgagaccc cgtccgacgt gatggccgag gtggccgaga tgacgccgcg ttacgccggc
     5581 atctcgcacg agcgaatcgg ccgcgacggc ctgcagtggc cggtggcccc cgacggcacc
     5641 gacacgccga tcctgtacga ggagcacttc gagaccggcg acgggctggg ccacttcgcc
     5701 gccctcccgt acaagccgcc cggcgacgcg gccgacgatg agttcccgtt catcctggtc
     5761 accgggcgcc ggctcgagca ctacaacgcc ggcacgatga cccgccgcac cggcaacctg
     5821 acgctgatgg accacgactg gctcgaggtg cacccggacg acgccgcgaa gct
//
LOCUS       DASUPW010000293         3892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1039497, whole genome shotgun sequence.
ACCESSION   DASUPW010000293 DASUPW010000000
VERSION     DASUPW010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039497"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VFY44_06625"
     CDS             <1..676
                     /locus_tag="VFY44_06625"
                     /inference="COORDINATES: protein motif:HMM:NF024684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910151.1"
                     /translation="FRTKDIAEMQRASLSEAMRHPTWNMGAKITIDSATLMNKGLEVI
                     EAHHLFGLGYDRIDVVVHPQSIVHSLIHLADGASLAHLGYPDMRVPISYALHFPDRAD
                     VPVRRLDLAELGELTFEAPDPETFACLRLAREAGEAGGTAPCVLNAANEIAVQAFLDE
                     RLSFAGIARVIETTLDSMPVAPVGHFSDLFRVDEEARERAREALAELAPGASSRPGRM
                     RSGDLP"
     gene            673..1761
                     /locus_tag="VFY44_06630"
     CDS             673..1761
                     /locus_tag="VFY44_06630"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEX5910152.1"
                     /translation="MSGLSWILAFAGFAFLVIAHELGHFTVAKLVGMRVEKFSLFFPP
                     TLASVKRGETEYAIGAIPAGGYVKISGMNPDEDLPEEVRDRAYHAQPVWKRIAVIAAG
                     PAVNLVLAFILLFVFFAFVGAEGPSTDKVQVTEKGYPASAALKPGDRIVSVDGVRGGP
                     VKLSPQIARHKCAGTPEKGCRAATPAELVVERGGKRREIAVRPIYDPAQKRTRLGFAF
                     APGPDVTLPPGEALTTSLDRFWFVTKQTVALPARLFDAQQRKEISGIVGSYEVTRQTI
                     LVDFSQVVAILALISLSLAIINLFPFLPLDGGHIFWAIVEKVRGKPVPLAVMERAGVV
                     GMVLVLGLFVLGLSNDIDRLTGEGFQVR"
     gene            complement(1806..2543)
                     /locus_tag="VFY44_06635"
     CDS             complement(1806..2543)
                     /locus_tag="VFY44_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910153.1"
                     /translation="MIRKITTALVLATALAAAAGPATADAANPRAGMKNAFFKVTVDG
                     VQTTTWKTDHPGSGGCDGALQGNGTEKVRFTSRPTTVRAVTLKGLNAPLLTTPKSIVD
                     VKLRLHGKVTRAGVLDAAPGGQCGGAGGGSIPRDCGAKSFRGVKLPLSYGLGTRGKDN
                     LDLRAEPIQDPFLNCPSGGSAFPTLVGTREGGPMPAALPRSELFDRKLGKIIVIARGQ
                     ESETVGEHTYLTKTRWVVTFERKVNRR"
     gene            complement(2582..3046)
                     /locus_tag="VFY44_06640"
     CDS             complement(2582..3046)
                     /locus_tag="VFY44_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910154.1"
                     /translation="MKRTITALAVTAVAAAGAGDAAAKSTQYKGKTKYGNTITFKRSG
                     GKITQLKTLVPATCMMVTSRGGGPPRSRAELFTPPGSFRIGAENKAVVQEDSAVTSDK
                     VTKHYRITLGRGARGRVTGKLHVNYSWQTVGWSSLLPWICQGDDAFSVAPVR"
     gene            3160..>3892
                     /locus_tag="VFY44_06645"
     CDS             3160..>3892
                     /locus_tag="VFY44_06645"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX5910155.1"
                     /translation="MTGSGSTIRLCGRLEAEIEGRRVEEALPGRQGRLLFAFLVLGRD
                     RPVRREQLLEVLWPEDGPPPSGDSLLAPPLSRLRKALGEGRLQGREQLSLDLPEDTWV
                     DWEAAWAHLAACRTALAGSEWRAAWDEGRAAVQIAGAGLLPGLEAPWIDQRRGELDEL
                     RVEALEAVATSGARLGGAEAAQAEEAARAAIEQAPYRETARVALIAALRAAGNDAEAL
                     RAYEDVRVLLRDELGTTPGPALLAAH"
BASE COUNT          488 a         1342 c         1458 g          604 t
ORIGIN      
        1 attccgtacg aaagacatag ccgaaatgca gcgtgcgagc ttgtcggaag caatgcgcca
       61 tcccacgtgg aacatgggcg ccaagatcac gatcgactcc gccaccttga tgaataaggg
      121 gctcgaggtg atcgaggccc accacctctt cggcctgggc tacgaccgca tcgacgtggt
      181 cgtgcacccg cagtcgatcg tccactcgct gatccatctg gccgacgggg cgtcgctggc
      241 ccacctgggc taccccgaca tgcgcgtgcc gatctcctac gcgctgcact tccccgatcg
      301 cgcggacgtg cccgtgcgcc gcctggacct ggccgagctg ggcgagctga ccttcgaggc
      361 ccccgacccg gagaccttcg cgtgcctgcg cctggcccgg gaggcggggg aggccggcgg
      421 cacggcgccg tgcgtgctga acgcggccaa cgagatcgcc gtgcaggcgt tcctggacga
      481 gcggctctcg ttcgcaggca tcgcccgcgt gatcgagacc acgctggact cgatgccggt
      541 ggcgccggtc gggcacttct ccgacctgtt ccgggtcgac gaggaggccc gcgagcgcgc
      601 ccgtgaggcg ctggcggagc tcgcgccggg cgcaagcagc cggcctggcc ggatgcgcag
      661 cggggacctt ccatgagcgg cctgagctgg atcctggcct tcgcgggctt cgccttcctg
      721 gtgatcgcgc acgagctggg ccacttcacc gtggccaagc tggtgggcat gcgggtggag
      781 aagttctcgc tgttcttccc cccgacgctg gcctcggtca agcgcggcga gaccgagtac
      841 gccatcggcg cgatccccgc cggcggctac gtgaagatca gcggcatgaa cccggacgag
      901 gacctccccg aggaggtccg cgaccgggct tatcacgccc agccggtgtg gaagcggatc
      961 gcggtgatcg cggcgggccc ggccgtgaac ctggtcctgg ccttcatcct gctgttcgtc
     1021 ttcttcgcct tcgtgggcgc cgagggcccg tcgaccgaca aggtccaggt caccgagaag
     1081 ggctatcccg cgtcggccgc gctcaagccc ggcgaccgga tcgtctccgt cgacggcgtc
     1141 cgcggcgggc ccgtcaagct ctcgccgcag atcgccaggc acaagtgcgc cggcaccccg
     1201 gagaagggct gccgggcggc cacgccggcg gagctggtgg tcgagcgcgg cggcaagcgg
     1261 cgcgagatcg cggtgcggcc catctacgac ccggcccaga agcgcacccg gctgggcttc
     1321 gcgttcgcgc cgggcccgga cgtgacgctg ccgcccggcg aggccctgac cacgtcgctg
     1381 gaccggttct ggttcgtgac caagcagacc gtggcgctgc ccgcgcgcct gttcgacgcc
     1441 cagcagcgca aggagatctc cggcatcgtc ggctcctacg aggtcacgcg ccagacgatc
     1501 ctcgtggact tcagccaggt cgtggccatc ctggccctga tctcgctgtc gctggccatc
     1561 atcaacctgt tcccgttctt gccgctggac ggcgggcaca tcttctgggc gatcgtggag
     1621 aaggtgcgcg gaaagccggt gccgctggcc gtgatggagc gggcgggggt ggtcggcatg
     1681 gtgctcgtgc tggggttgtt cgtgctcggg ttgtcgaacg acatcgatcg ccttaccggc
     1741 gaggggttcc aggtcagata gcggcgcggg gcggcgcacg gtgcgccgcc ccgcgagccg
     1801 cctcgtcagc ggcgattcac cttccgttcg aaggtcacca cccagcgagt cttcgtcagg
     1861 taggtgtgct cgccgacggt ctccgactcc tgtccccggg cgatcacgat gatcttgccc
     1921 agcttccggt cgaagagttc ggaccgcggc agggcggccg gcatggggcc gccctcccgg
     1981 gtgcccacca gcgtcgggaa cgccgagccg ccggaggggc agttgaggaa cgggtcctgg
     2041 atgggctcgg cccgcaggtc gaggttgtcc ttgccccggg tgcccagccc atacgacagc
     2101 gggagcttga cgccgcggaa ggacttggcc ccgcagtcgc gcgggatcga gccgccaccc
     2161 gcgccgccgc actgaccgcc gggggcggcg tccagcacgc ccgcccgggt gaccttcccg
     2221 tgcagccgga gcttgacgtc cacgatcgac ttcggcgtgg tcagcagcgg ggcgttcagg
     2281 cccttcagcg tcacggcgcg gacggtcgtg ggccgcgagg tgaaccgcac cttctcggtg
     2341 ccattcccct gcagggcgcc gtcgcagccg cccgaccccg ggtggtcggt cttccaggtg
     2401 gtggtctgca cgccgtcgac ggtgaccttg aagaaggcgt tcttcatgcc cgccctcggg
     2461 tttgcggcgt cggcggtggc gggcccggcc gccgcggcga gcgcggtggc caggacgagg
     2521 gctgtggtga tcttgcggat catgtctgtt ctcctgggag gtagaggggt ggccggcgcg
     2581 gtcaccggac gggggcgacg ctgaaggcgt cgtcgccctg gcagatccac ggcagcaggc
     2641 tcgaccagcc cacggtctgc cacgagtagt tcacgtgcag cttgccggtg accctgccgc
     2701 gggctccgcg cccgagggtg atccggtagt gcttggtcac cttgtcggag gtcacggccg
     2761 agtcctcctg gaccacggcc ttgttctcgg cgccgatccg gaacgagccg ggcggtgtga
     2821 acagctcggc gcgggacctc ggaggaccgc cgcccctgct ggtgaccatc atgcaggtcg
     2881 cgggcaccag cgtcttgagc tgggtgatct tcccgcccga tcgcttgaac gtgatggtgt
     2941 tgccgtactt ggtcttgccc ttgtactggg tggacttggc ggctgcgtcg ccggcgcccg
     3001 ctgcggctac tgcggtgacg gccagtgcgg tgatggtgcg cttcatggtg gtctcctctt
     3061 gctgggtggt ggtgactgcg tggcccaccc tgcgggcccg gccttgcgcg gcccttgcgg
     3121 cgaccttgcg acgccgttgc cgccggtagc gtgcgcgctg tgacgggctc agggagcacg
     3181 atccggctct gcggccgcct ggaggcggag atcgagggca ggcgcgtcga ggaggccctg
     3241 ccgggccgcc agggacggct cctgttcgcc ttcctggtgc tgggccgcga ccggcccgtc
     3301 cgccgcgagc agctgctcga ggtgctgtgg cccgaggacg gcccgccgcc gagcggcgac
     3361 tcgctgctgg ccccgccgct gtcgcgcttg cgcaaggcgc tgggggaggg gcggctccag
     3421 ggccgcgagc agctctcgct cgacctgccc gaggacacct gggtcgactg ggaggcggcg
     3481 tgggcgcacc tggcggcatg tcgcacggcc ctggcgggat cggaatggcg cgcggcgtgg
     3541 gacgagggcc gggccgccgt ccagatcgcc ggcgcggggc tgctgcccgg gctggaggcg
     3601 ccgtggatcg accagcgccg cggcgagctg gacgagctgc gggtggaggc cctggaggcc
     3661 gtcgccacgt ccggagcacg gttgggcggg gccgaggccg cgcaggccga ggaggcggcg
     3721 cgggcggcca tcgagcaggc gccctaccgc gagaccgccc gggtggccct gatcgcggct
     3781 ctgcgcgccg cggggaacga cgccgaggcg ctgcgcgcct acgaggacgt ccgcgtgctc
     3841 ctgcgcgatg agctgggcac cacgccgggg cccgcgctgc tggccgccca cg
//
LOCUS       DASUPW010000294         2009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1039677, whole genome shotgun sequence.
ACCESSION   DASUPW010000294 DASUPW010000000
VERSION     DASUPW010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039677"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1519
                     /locus_tag="VFY44_06650"
     CDS             200..1519
                     /locus_tag="VFY44_06650"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX5910156.1"
                     /translation="MRSILLAFALVLVLPATALAAGTLTVAISGAGAVSGSGIECTKA
                     PGAGVAGPCSVPLTGPATVTAAPAAGFRFEGWTGACEGTAPSCELDMDGDRSVAAGFR
                     DIQPPVVTLAEPLAGSVKGPMPISAEVTDNGQIEKVEFKVGTFAWVDKVAPYSAIWPT
                     VPFPDGPVPVTATAYDKAGNFHTKQVEVLFDNTAPVVGATGPDRQAFAPGSTQTWQVT
                     ARDATSSFETRCSVVPQGSARVIVPCTGGSSHSVSGLPVGRYEFVFAARDAAGNYAET
                     VRLFRIETPGTGESAETTAFDGELDPAPAPAPEAAAALAAPATAAADAPPKIAVALGF
                     RYASLSRATKLSNFVVRGVPAGATVTVSCVKGCTKKALKRFVKRAGRISLKALARRPL
                     KVDTTITVIVSKPGHTSAVKVLEIRARKAPLVTTQCQPEGASEPSAC"
     gene            1599..>2009
                     /locus_tag="VFY44_06655"
     CDS             1599..>2009
                     /locus_tag="VFY44_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910157.1"
                     /translation="MSTFIEPVLGALGLRPLVPAGLVGLLAGALLFAGSAQAGTVTLT
                     PTIEGAGKLTSNGSGTYPCSLLLPAGAQPKNTDTTVCAATSRAAFAIPDIRTGGIIYL
                     NNPLVVVAEANPGWRFMGWKNCTPVQGSGPSGLAC"
BASE COUNT          272 a          746 c          696 g          295 t
ORIGIN      
        1 cagctctacg aggcgctgcg cggggaggag gtcgacgcgg ccaacttccg ccgcgacgtc
       61 cgcgccaccg ggctgctcga ggacaccggc tcgctgcgct cggagggccc gggccggccg
      121 ggtcgcctgt accggcgcgc ctgaggcgct ccaagaatcc gccaagcgtg tcccaagccg
      181 ggagcgattg ggtgtgcacg tgcgctcgat ccttctcgcc ttcgcgctgg tccttgtcct
      241 tccggcgacc gcgctggccg ccgggaccct gaccgtcgcg atctccggcg ccggtgcggt
      301 atcggggagc gggatcgaat gcacgaaagc tcccggcgcg ggcgtggccg ggccgtgctc
      361 ggtgccgctg accggtcccg cgacggtgac ggcggcgcct gcggccgggt tccgcttcga
      421 gggctggacc ggcgcctgcg agggcaccgc tccctcgtgc gagctcgaca tggatggcga
      481 ccggtcggtc gccgcgggct tccgtgacat tcagccgccc gtggtgacgc tcgcggaacc
      541 gctggccggg tcggtgaagg gcccgatgcc gatctccgcc gaggtcaccg acaacggcca
      601 gatcgagaag gtcgagttca aggtcggcac gttcgcgtgg gtggacaagg tggcccccta
      661 ctccgccatc tggccgaccg tgcccttccc ggacgggccg gtgcccgtga ccgcgaccgc
      721 ctacgacaag gccggcaact tccacacgaa gcaggtcgag gtcctgttcg acaacaccgc
      781 gccggtcgtc ggcgccaccg ggccggatcg ccaggcgttc gcccccgggt cgacgcagac
      841 ctggcaggtc accgccaggg atgcgacgtc ctccttcgag acgcgctgca gcgtcgtgcc
      901 ccagggctcc gcgcgggtca tcgtcccctg caccggcggc agttcccaca gcgtcagcgg
      961 gctccccgtg gggcgctacg agttcgtctt cgccgcccgc gacgccgccg gcaactacgc
     1021 cgagaccgtg cggctcttcc ggatcgagac gcccggcacg ggggagtccg ccgagacgac
     1081 ggccttcgac ggcgagctcg accccgctcc ggcgccggct cctgaagctg ccgccgcgct
     1141 cgccgcgcct gccacagccg ccgccgacgc gccgccgaag atcgccgtcg ccctcggctt
     1201 ccgctacgcc tccctgagcc gcgcgaccaa gctctcgaac ttcgtcgtcc gcggcgtgcc
     1261 cgccggcgcc accgtgacgg tcagctgcgt gaagggctgc accaagaagg cgctcaagcg
     1321 cttcgtgaag cgcgccggcc ggatctcctt gaaggcgctc gcgcgccgcc ccctgaaggt
     1381 cgacacgacg atcacggtga tcgtctccaa gcccgggcac acgtccgcgg tcaaggtcct
     1441 cgagatccgc gcccgcaagg cgccgctcgt gaccacgcag tgccagcccg agggcgcttc
     1501 tgagccgagc gcctgctgac tggacgagtt gcggatatcg aaaaccggcc gcgcccctag
     1561 tgcggctgct gcttagagcc cagggaggat ggaggatcgt gtcgacgttc attgagccgg
     1621 tgcttggcgc gttggggttg cggcctcttg tgcccgcggg acttgtcggc ctgcttgccg
     1681 gggcgctctt gttcgctggt tccgcgcagg cgggaacggt gaccctcacg cccaccatcg
     1741 agggcgccgg caagctcacg agcaacggct ccggcacgta cccctgctcg ctgttgctcc
     1801 ccgccggcgc gcaacccaag aacaccgaca cgaccgtctg cgcggccacg agccgtgcgg
     1861 cgttcgcgat cccggacatc cggaccggcg gaatcattta cctcaacaac ccgctggtcg
     1921 tggtggccga agcgaacccc ggctggcgct tcatgggttg gaagaactgc acgccagtgc
     1981 agggctccgg cccctccggg ctggcgtgc
//
LOCUS       DASUPW010000295         4188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1456031, whole genome shotgun sequence.
ACCESSION   DASUPW010000295 DASUPW010000000
VERSION     DASUPW010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1456031"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="VFY44_06660"
     CDS             complement(<1..1163)
                     /locus_tag="VFY44_06660"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5910158.1"
                     /translation="MIAKVEPLTTARALRGPFDYLLGEGMGDVGVGSMLVVPFARRRI
                     LGVVVDVAEHSDVPPDKLAEPLSALEADVPPELVNLGLWVAREYVSTPARGLALVLPP
                     GTGTGSGRRIGPRRSLRASITAAGREALEGDARLGTRQRAALEALAAGPARVSDLASA
                     TGCDHAGARRLEGRGLLTLEYADQAPARPRTSMVGARPDVRALTGAQEAALAEVVTAM
                     DAPAGTDRRLLLHGVTGSGKTEVYLRAVAAARERGRTAIVLVPEIALTPQTAGRFVQR
                     FGDTVAVLHSQLTARERYDEWARLRSGAATVCVGPRSAVFAPLTGVGLIVVDEEHDPS
                     YKHEGDPRYDARLVAARRGAKVVFGSATPRPESAAALRRLRLGERVDGRALPPV"
     gene            complement(1160..1912)
                     /locus_tag="VFY44_06665"
     CDS             complement(1160..1912)
                     /locus_tag="VFY44_06665"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5910159.1"
                     /translation="MEATGSAELRDVYEEQYSAGDADAARYGRWRALCAEGKADHVVR
                     LAAELDRPPAAVAEVGCGDGVLLELMARRGLGETHHGYEISERAVQIATDRLGPGAVE
                     RFDGEHLPVADATYDLGVLSHVLEHVPDPLPLLRETGRAAGAVVVEVPLEDNRSASRP
                     EAERARQEIGHLHRFNRGDIHVLCAAAGLRVVAELADPLPLKVHTFFAESASARAKAV
                     AKAAVRRGVYVASAKAAERSFTVHYACLAVSR"
     gene            1939..3060
                     /locus_tag="VFY44_06670"
     CDS             1939..3060
                     /locus_tag="VFY44_06670"
                     /inference="COORDINATES: protein motif:HMM:NF028011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase regulatory domain-containing
                     protein"
                     /protein_id="HEX5910160.1"
                     /translation="MATDFEADGLLEGLEGQAREARLELLRQLESEGVPTEELRAASA
                     EGRLALVPVERILVGDSPHYTAAEVAERSGMEPEFLDRYWRAIGMTSAGPDDPAFVDD
                     DVDAARRLDDLRDAGLGDDEIIEMARVMSSGMSTLAVTIARVFADTYLQEGDDEASLS
                     VRFAEAAQKLLPLLGPALEHALMVQNRSNLRQASAIGSSLAEGRLPDQQEVSVAFADL
                     VDFTKLGESVDPGRLGAVAAQLEELTRDVIRRPVRLVKTIGDEVMLESADTVALLEAA
                     LDLLDAADRQDDEFPQIAVGIARGPAISRGGDLFGHSVNLASRVTATARPGSVLVTQE
                     VRDAAGEDAYRYSFAGERKLKGVKDQQKLFRVRRVQGDD"
     gene            3109..>4188
                     /locus_tag="VFY44_06675"
     CDS             3109..>4188
                     /locus_tag="VFY44_06675"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5910161.1"
                     /translation="MNSNQPYPNRWKALVVLALSLLIVSLDNTILNTALPSLRDDLGA
                     SGSALQWIVDSYLLVFAGLLLTAGSLGDRFGRKRALQFGLLVFAVGSTASALATSSEM
                     LTATRALMGVGGAFIMPSTLSIITAIFPAEERGKAIGAWAAVSGLGIVLGPVVGGALL
                     KAFDWSAVFWVNVPIAAIAIVAGMRLIPESRDPRARPLDLRGAALSMAGLSAVVWSII
                     EAPERGWTSPAVLAATAAGVAVLAGFAWWERRARYPMLDLALFRDLRFSAASITISLL
                     FAALMGTVFILTQHLQSVLDYDALGAGLRITPLGAGVIVASAASARLTDRVGTKVMVA
                     GGMSVIAAGLGTLSLVTVDSGYGLIA"
BASE COUNT          526 a         1564 c         1537 g          561 t
ORIGIN      
        1 accggtggga gcgcacggcc gtcgacccgc tcaccgagcc gcagccgccg cagcgcggcg
       61 gccgactccg gccgcggtgt cgcgctgccg aagacgacct tcgccccgcg gcgcgccgct
      121 acgaggcgcg cgtcgtagcg cgggtcgccc tcgtgcttgt acgagggatc gtgctcctcg
      181 tcgaccacga tcaggcccac cccggtcagc ggcgcgaaga ccgccgaccg tggccccacg
      241 cagaccgtgg ccgcgcccga gcgcagcctg gcccactcgt cgtagcgctc ccgtgcggtc
      301 agctgcgagt gcagcacggc gacggtgtcg ccgaagcgct gcacgaaccg gccggcggtc
      361 tggggcgtca gcgcgatctc gggcaccagc acgatggcgg tgcggccacg ctcgcgggcg
      421 gcggcgaccg cgcgcaggta cacctcggtc ttgccgctgc cggtgacgcc gtgcaggagc
      481 aggcgccggt cggtgccggc gggggcgtcc atcgcggtca cgacctcggc caacgcggcc
      541 tcctgcgccc cggtgagcgc ccgcacgtcg ggccgcgcgc ccaccatcga cgtccgtggc
      601 cgcgccgggg cctggtcggc gtactccagc gtcagcaggc cgcgtccttc caggcgccgt
      661 gcgccggcgt ggtcgcagcc ggtggccgag gccaggtcgc tgacccgcgc ggggccggcg
      721 gccagcgcct ccagcgccgc gcgctggcgg gtgcccagcc gcgcgtcgcc ctccagggcc
      781 tcacgcccgg ccgcggtgat ggacgcgcgc agcgaccgcc gcggcccgat ccggcgcccg
      841 ctgccggtgc ccgtgcccgg cggcagcacc agcgccaggc cgcgcgccgg cgtggacacg
      901 tactcgcggg ccacccacaa ccccaggttg accagctcgg ggggaacgtc ggcctccagc
      961 gccgacagcg gctcggccag cttgtcgggc ggcacgtcgc tgtgctcggc cacgtcgacc
     1021 accacgccga ggatgcggcg gcgggcgaac gggaccacca gcatcgaccc cacccccacg
     1081 tcgcccatgc cctcgcccag gaggtagtcg aacggcccgc gcagcgcccg cgcggtggtc
     1141 agaggctcga ccttggcgat caccgggaga cggccaggca ggcgtagtgc accgtgaacg
     1201 accgctccgc ggccttcgcg gacgccacgt acacgccccg gcgcacggcc gccttggcca
     1261 ccgccttcgc ccgcgccgac gccgactcgg cgaagaacgt gtgcaccttc agcggcagcg
     1321 ggtcggccag ctctgccacc acgcgcaacc cggcggcggc gcacagcacg tggatgtcgc
     1381 cgcggttgaa gcggtgcagg tgcccgatct cctgccgcgc gcgctcggcc tcgggccggg
     1441 acgcgctgcg gttgtcctcc agcggcacct ccaccaccac cgcgccggcg gcccggccgg
     1501 tctcgcgcag cagcggcagc gggtcgggca cgtgctcgag cacgtgcgag agcacgccca
     1561 ggtcgtaggt cgcgtccgcg accggcaggt gctcgccgtc gaagcgctcg accgcgccgg
     1621 gtcccaggcg gtcggtggcg atctgcaccg cccgctcgga gatctcgtag ccgtgatggg
     1681 tctcccccag gccgcgccgc gccatcagct ccagcaggac gccgtcgccg cagcccacct
     1741 cggccacggc cgccgggggg cggtccagct cggcggccag gcgcaccacg tggtcggcct
     1801 tgccctcggc gcagagggcc cgccagcgcc cgtagcgcgc ggcatccgcg tcgccggcgc
     1861 tgtactgctc ctcgtagacg tcgcgcagct ccgccgaccc ggtggcctcc attggccgta
     1921 ggctacgcgg cccttacgat ggccaccgac ttcgaagcag acgggctcct ggagggcctt
     1981 gagggccagg cgcgcgaggc gcggctggag ctgttgcgac agctggagtc cgagggcgtg
     2041 cccaccgagg agctgcgcgc ggcctccgcc gagggccggc tggccctggt gccggtggag
     2101 cggatcctgg tcggcgactc gcctcattac accgccgccg aggtggcgga gcgctcgggc
     2161 atggagcccg agttcctgga ccgctactgg cgggcgatcg gcatgaccag cgccggcccc
     2221 gacgacccgg ccttcgtgga cgacgacgtg gacgccgccc ggcgcctgga cgacctgcgt
     2281 gacgccggcc tgggcgacga cgagatcatc gagatggccc gcgtcatgag ctcgggcatg
     2341 agcacgctgg cggtgaccat cgcccgcgtg ttcgccgaca cctacctcca ggagggcgac
     2401 gacgaggcca gcctgtcggt gcgcttcgcg gaggccgccc agaagctgct tcccctgctg
     2461 ggaccggcgc tggagcacgc gctgatggtg cagaaccgca gcaacctgcg ccaggcgtct
     2521 gccatcgggt cgtcgctggc cgaaggccgt ctgcccgacc agcaggaggt ctcggtggcc
     2581 ttcgccgacc tggtggactt cacgaagctg ggcgagagcg tggacccggg ccgcctgggc
     2641 gccgtggccg cgcagctgga ggagctgacg cgggacgtga tccgccggcc cgtgcggctg
     2701 gtcaagacca tcggcgacga ggtcatgctc gagtcggccg acaccgtggc cctgctggag
     2761 gccgcgctgg acctgctgga cgccgccgac cgccaggacg acgagttccc gcagatcgcg
     2821 gtgggaatcg cgcgcggacc ggccatctcg cgcggcggtg acctgttcgg gcactcggtg
     2881 aacctggcca gccgggtcac cgccaccgcg cggccgggca gcgtgctggt gacccaggag
     2941 gtcagggacg cggccgggga ggacgcctac cgctactcgt tcgccggtga gcgcaagctc
     3001 aagggcgtga aggaccagca gaagctcttc cgggtgcgca gagttcaggg tgacgattaa
     3061 gggggtcccc ccgatgttct agggccgcgc cggcgggacg ctgggcccat gaactcgaac
     3121 cagccatatc ccaatcggtg gaaggcactg gtggtcctgg ccctcagcct tctgatcgtc
     3181 tccctggaca acaccatcct caacacggcg ctcccctcgc tgcgggacga cctcggcgcg
     3241 tcgggcagcg cgctgcagtg gatcgtcgac tcgtatctgc tcgtgttcgc cggcctgctg
     3301 ctgacggccg gatccctggg cgaccgcttc ggccgcaagc gcgcactgca gttcggcctg
     3361 ctggtgttcg cggtcgggtc cacggcctcg gccctggcca cctcgagcga gatgctgacc
     3421 gcgacacggg cgctgatggg cgtcggcggc gcgttcatca tgccctcgac cctgtcgatc
     3481 atcaccgcga tcttccccgc ggaagagcgt ggcaaggcga tcggcgcctg ggcggccgtg
     3541 tcgggcctgg gcatcgtgct cgggccggtg gtcggcggcg ccctgctgaa agcgttcgac
     3601 tggagcgcgg tgttctgggt caacgtgccc atcgcggcga tcgccatcgt cgccggcatg
     3661 cggctgatcc ccgagtcccg cgacccgcgg gcccgcccgc tggacctgcg cggcgcggcg
     3721 ctgtcgatgg ccgggctctc cgcggtggtg tggtcgatca tcgaggcccc cgagcgcggc
     3781 tggacctcgc ccgcggtgct ggccgccacg gctgccggag tcgccgtgct ggccgggttc
     3841 gcctggtggg agcggcgcgc gcgctacccg atgctggatc tggccctgtt ccgcgacctg
     3901 cggttctcgg cggccagcat cacgatcagc ctgctgttcg ccgcgctgat gggaaccgtg
     3961 ttcatcctga cccaacacct gcagtcggtg ctggactacg acgcgctggg cgccggcctg
     4021 agaatcactc ccctgggcgc cggcgtgatc gtggcctccg ccgcctcggc gcggctgacc
     4081 gaccgcgtgg gaacgaaggt catggtggcc ggaggcatgt cggtgatcgc cgccgggctg
     4141 ggcacgctct cgctggtcac cgtggacagc ggctacgggc tcatcgca
//
LOCUS       DASUPW010000296         4930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1733647, whole genome shotgun sequence.
ACCESSION   DASUPW010000296 DASUPW010000000
VERSION     DASUPW010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1733647"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..1231
                     /locus_tag="VFY44_06680"
     CDS             209..1231
                     /locus_tag="VFY44_06680"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HEX5910162.1"
                     /translation="MNESTQKVVQYLNEAHATELALTRVLQSQIAMTPAGTYRNGLEK
                     HLGETQDHADRVQRRLGELDAGGNPLQVVVGVAEGVLGQVLAFAKTPLDLVRGTGGEE
                     KVLKNAKDACATEALEIATYTALERLARSVGDTKTADLAASIRQDEQDMLDRVLAEIP
                     KLAAAVVGAEVDGDPSFDVTKTGAADAARTTKKTAGKAARNGKARAKSTARQARKVPG
                     VAQAEGQAKGAVAGEGDLAIARYDSLTADDITSKLTELSQIDLAKVDSYERKNQNRTT
                     VLDRVTTLRGDEPWPSYDEQSVSEITTALRAADDDSTREVVAYERAHKNRAGVLKAAE
                     RDAAKA"
     gene            1323..2309
                     /locus_tag="VFY44_06685"
     CDS             1323..2309
                     /locus_tag="VFY44_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910163.1"
                     /translation="MLPTRFAALLGASVVLSLTGAAVSASSAAAAGHITYSVGEGPLL
                     RVEAKAGGKPQNVSAGLDRRFPGGGDQFLNQSPDGKFLIIQSERFGCAGNPCLAVVSS
                     DFRSGSGLPGKTAEGFSAVGNGGRVVVYPAQSSGGRIDLFVSRRTGGGWSAPRNITAK
                     SSKKAHVHPAIATNSKRVLHDCGNAPDPSSGTLSICETRLDGGGTRVRMAPSAGRKPQ
                     DPNFGPGSLHHGDYLGRGKGLVFESQWCCEGESIWWLPKGKRKPRHVRGASGNNVAPC
                     GLPDGRVVSLDITRAAGNGVHELMITSPTSKKRTFVLPPGVDVNDIGIGCGR"
     gene            complement(2316..2522)
                     /locus_tag="VFY44_06690"
     CDS             complement(2316..2522)
                     /locus_tag="VFY44_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910164.1"
                     /translation="MPTATQLGQSGLQGWREKLADGVAEPVAERVPLDEDQVRAAIGA
                     LFFVLSVIYVVNTVRAAAGKARSS"
     gene            complement(2582..3070)
                     /locus_tag="VFY44_06695"
     CDS             complement(2582..3070)
                     /locus_tag="VFY44_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910165.1"
                     /translation="MAFFRILVATAALAGLAAPAAEAARAPAPLKAATAAAQRYWGVT
                     PCNGQVAVVPRSRLQAGMDRTTDAWATFSTPLGWNNLTAPAAGYRNCTISLARWRWPT
                     TASMTADWELLCATVIHETGHLLGRPHDARRGSIMAPVFDDASSLPRICRTARPAAKR
                     SR"
     gene            3229..3834
                     /locus_tag="VFY44_06700"
     CDS             3229..3834
                     /locus_tag="VFY44_06700"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5910166.1"
                     /translation="MTPSPPITVALAGFDDLLTLGLRALLADDPALSVVGEVVGYELL
                     SPTLRDLRPDVLIADAGALRDLAQVRELTVEHPGTHLVLFAEDVAAAERAQLLAFGAS
                     ACLSWKTQARDVRSAIHLASRGMQLTPLASHLPNGGAEREPLLTPREGDVLLLLRRGL
                     GNGQIALELQIGVETVRTHARHIYRKLGVSSRRALLALPSA"
     gene            complement(3824..4543)
                     /locus_tag="VFY44_06705"
     CDS             complement(3824..4543)
                     /locus_tag="VFY44_06705"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX5910167.1"
                     /translation="MSRLTSTATAVGVALATMGVCSAAAAPAPAAAATAESTRLVVLL
                     KGHVARSRPSLGGRRLEYVPARRPLTHVRTVLPVIGRRVRGGRSWVHVRLPGRPSGHT
                     GWIRGGQTRPSSTPWRIRVRLSSRRVAVYHEGRVVRRFRAIVGTPSTPTPRGRFFVEE
                     ALALSPSAHGGPFALATSARSSVYQEFEGGPGQIAMHGTNGLSGSLGTAASHGCIRLS
                     PRAITWLARRVAGGTPLRVTR"
     gene            complement(4540..>4930)
                     /locus_tag="VFY44_06710"
     CDS             complement(4540..>4930)
                     /locus_tag="VFY44_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910168.1"
                     /translation="ATGAPGNNAGTGNQPPPASRTTRRLRRGTSTLRRTRNPAPGATC
                     TDGFTARVRGAYVKSVVFRLDGKRIATKRRSPFSAYVKAAGAGRHKVTAKVTYRDATR
                     AVTRTLRYRGCAAAVLQPRRGPSQFTG"
BASE COUNT          688 a         1819 c         1786 g          637 t
ORIGIN      
        1 aagcgctttg ccagcagcgc cgcgcttgct cgtcacgccc gagaagtcgt cgcgcccacc
       61 gtgacgagat cccaactccc tgatggcccg cccgttcacc tggcgagccg tttctcgtac
      121 ccgggacgcc gtgatcgatc ggaacccagc cggcgtaccc ctagctcccc gcaccaccca
      181 acttcactcc cacgacagga agccacacat gaacgagtcc acacagaagg tcgtccagta
      241 cctcaacgaa gcccacgcga ccgagctcgc gctgacccgc gtgctgcagt cccagatcgc
      301 gatgaccccg gccggcacat accggaacgg gctcgagaag cacctgggcg agacccagga
      361 tcacgccgac cgggtgcagc gacgcctcgg tgagctcgat gcgggcggga acccgctcca
      421 ggtcgtggtc ggagtcgccg aaggagtgct cgggcaggtc ctggccttcg ccaagacccc
      481 gctggatctg gttcgcggca ccggcggtga ggagaaggtg ctcaagaacg ccaaggacgc
      541 ctgcgccacc gaggccctcg agatcgccac ctacacggcg ctcgagcgac tggcccgctc
      601 ggtgggcgac accaagaccg ccgatctggc cgcctcgatc cgccaggacg agcaggacat
      661 gctcgaccgg gtgctcgcgg agatcccgaa gctcgcggcc gccgtcgtgg gcgccgaggt
      721 ggacggggac ccctcgttcg acgtgacgaa gaccggtgcg gccgacgccg cccgcacgac
      781 gaagaagacg gccggcaagg ccgcccgcaa cggcaaggca cgggccaaga gcaccgcgcg
      841 ccaggcgcgc aaggtgcccg gcgtcgcgca ggccgagggc caggccaagg gcgccgtcgc
      901 cggcgagggc gacctcgcga tcgcgcgcta cgactccctc accgccgacg acatcaccag
      961 caagctcacc gagctgtcgc agatcgacct ggccaaggtc gacagctacg agcgcaagaa
     1021 ccagaaccgc acgacggtgc tggaccgggt caccaccctc cgcggtgacg agccgtggcc
     1081 ctcgtacgac gagcagtcgg tctccgagat cacgaccgcc ctgcgcgcgg ccgacgacga
     1141 ctccacccgt gaggtcgtcg cctacgagcg tgcgcacaag aaccgcgccg gcgtgctcaa
     1201 ggccgccgag cgcgacgccg cgaaggcctg agctcgaggc ggcggccgcc cagccggggc
     1261 ggccgccgcc cgccttcgcc ctccgctagc ggcggtccgg cttctgacct atgttggccc
     1321 gcatgctgcc gacccgtttc gccgccctcc tgggcgcctc cgtcgtcctg tccctgaccg
     1381 gcgccgccgt ctcggcgagc tccgccgcgg cggccggcca catcacctac agcgtcggtg
     1441 agggtcccct cctgcgggtg gaggccaagg ccgggggcaa gccgcagaac gtgtcggcgg
     1501 ggctcgaccg ccgcttcccc gggggaggcg accagttcct caaccagtcg cccgacggca
     1561 agttcctgat cattcagagc gagcgcttcg gatgtgccgg caacccgtgc ctggcggtgg
     1621 tctccagcga cttccgctcc gggtcggggc tgccgggcaa gaccgccgag ggcttctcgg
     1681 ccgtgggcaa cggcggccgg gtcgtggtct acccggcgca gtccagcggg ggccgcatcg
     1741 acctcttcgt cagccgccgg acgggcggcg gctggagcgc gccgcgcaac atcaccgcca
     1801 agtcgtcgaa gaaggcccac gtccacccgg cgatcgccac gaactccaag cgcgtcctcc
     1861 acgactgcgg caacgccccc gacccctcgt cgggcaccct gtccatctgt gagacgcgcc
     1921 tggacggcgg gggcacccgc gttcggatgg ccccgtccgc cggccgcaag ccccaggacc
     1981 ccaacttcgg tcccggcagc ctccaccacg gcgactacct ggggcgcggc aagggcctgg
     2041 tcttcgagtc ccagtggtgt tgcgagggcg agtccatctg gtggctcccg aagggcaagc
     2101 gcaagcccag gcacgtccgc ggagccagcg ggaacaacgt ggccccgtgc ggcctgcccg
     2161 acggccgcgt ggtcagcctg gacatcaccc gcgccgcggg caacggggtc cacgagctga
     2221 tgatcacctc gccgacctcc aagaagcgca ccttcgtgct gccccccggt gtcgacgtga
     2281 acgacatcgg gatcggctgc gggcgatgac ggcggtcagc tcgaccgcgc cttgcccgca
     2341 gcggccctga ccgtgttcac cacgtagatc accgacagga cgaagaagag cgccccgatc
     2401 gcggcgcgca cctggtcctc gtccagcgga acacgctccg cgaccggctc ggcgactccg
     2461 tccgccagct tctcgcgcca gccctgaagg cccgactgtc ctagttgtgt agcggtgggc
     2521 atctctctcc tcgttctcgt ggccagtcag tgcatacccg ctcccgcgag ctacacccgg
     2581 cctatcgcga gcgcttcgcg gcaggacgcg cggtccggca gatgcgaggc aggctggatg
     2641 cgtcgtcgaa caccggcgcc atgatgctgc cgcgccgcgc gtcatggggg cggcccagca
     2701 ggtgcccggt ctcgtggatc accgtggcgc acagcagctc ccagtccgcg gtcatgctgg
     2761 ccgtggtggg ccagcgccag cgggccaggg agatcgtgca gttgcggtag ccggccgcgg
     2821 gggcggtcag gttgttccag cccagcggcg tgctgaaggt ggcccacgcg tcggtggtgc
     2881 ggtccatgcc ggcctgcagg cggctgcgcg ggaccacggc gacctggccg ttgcagggcg
     2941 tgacgcccca gtaacgctgg gcggcggccg ttgcggcctt cagcggcgcc ggggcccggg
     3001 ccgcctccgc cgccggggcg gccagtccgg caagggccgc ggtggcgacg aggatgcgga
     3061 agaaggccat gccttcaagc atgggtgggg catgcccgtc ccgcttcatc ccccgagggg
     3121 gatcttccct ggcctccggg tggggtcaca ggtgagggcc ggctcaccct ttgcccgcgc
     3181 gatgccgatg tacacagggg cggcgcgatc acgcgcgctc cgacctcgat gacgccatcg
     3241 ccacccatca ccgtcgccct cgcgggcttc gacgacctgc tcaccctggg gctgcgggcg
     3301 ctcctggccg acgacccggc gctgtccgtg gtcggcgagg tggtgggcta cgagcttctc
     3361 tcgccgaccc tgcgggacct gcgtcccgac gtgctgatcg ccgatgcggg cgcgctgcgg
     3421 gacctggcgc aggtccgcga gctgaccgtc gaacaccccg gcacgcacct ggtcctgttc
     3481 gccgaggacg tggccgccgc ggagcgggcc cagctgctgg ccttcggcgc cagcgcgtgc
     3541 ctgtcgtgga agacccaggc gcgcgacgtg cgcagcgcga tccacctggc ctcgcgtggc
     3601 atgcagctga ccccgctggc ctcgcacctg cccaacggcg gcgccgagcg cgagccgctg
     3661 ctgaccccgc gcgagggcga cgtgctccta ctgctgcggc gggggctcgg gaacggccag
     3721 atcgcgctcg agctgcagat cggcgtcgag acggtgcgca cgcacgcgcg ccacatctac
     3781 cgcaagctgg gcgtctcctc gcgccgcgcg ctgctggcgc tgcctagcgc gtgacccgca
     3841 gcggcgtgcc gccggccacc cggcgcgcca gccaggtgat ggcccgcggc gagaggcgga
     3901 tgcagccgtg ggaggcggcg gtgccgaggc tgcccgagag gccgttggtc ccgtgcatgg
     3961 cgatctggcc cgggccgccc tcgaactcct ggtagacgct ggaccgggcg ctggtggcca
     4021 gcgcgaacgg gccgccgtgc gcgctgggag agagcgccag cgcctcctcg acgaagaacc
     4081 ggccgcgcgg ggtgggggtg gagggcgtcc cgacgatggc gcggaagcgc cggacgacgc
     4141 ggccctcgtg atagacggcg acgcgtcgtg acgagagcct gaccctgatc cgccaggggg
     4201 tggacgacgg cctggtctgg ccgccgcgga tccagcccgt gtgcccgctg gggcggccgg
     4261 ggagccgcac gtggacccag gaccgcccgc cgcggacgcg gcggccgatc acgggaagca
     4321 cggtccgcac gtgcgtcagg gggcgccgcg cgggcacgta ctcgagcctg cgcccgccca
     4381 ggctggggcg ggagcgggcg acgtgcccct tcaggagcac gaccaggcgg gtgctctccg
     4441 ccgtcgcggc ggccgcgggg gccggcgcgg cggcggcgga gcacacgccc atcgtggcca
     4501 gcgcgacccc cacggccgtg gccgtggagg tcaggcggct catccggtga actgcgacgg
     4561 cccgcgtcgc ggctggagca ccgccgcggc gcagccgcgg tagcgcaggg tccgggtgac
     4621 ggcgcgggtg gcgtccctgt aggtcacctt ggcggtgacc ttgtgcctgc cggcgcctgc
     4681 tgccttgacg taggccgaga acggcgagcg gcgcttggtg gcgatgcgct tgccgtcgag
     4741 cctgaacacg acgctcttca cgtaggcgcc tcggaccctg gcggtgaagc cgtcggtgca
     4801 cgtggctccc ggtgcggggt tcctggtgcg tcgcagcgtc gacgtgcccc tacgcaggcg
     4861 gcgcgtggtc cggctggcgg gcggcggctg gttgccggtt cccgcgttgt tgccgggcgc
     4921 tcccgtagcg
//
LOCUS       DASUPW010000297         2133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1456443, whole genome shotgun sequence.
ACCESSION   DASUPW010000297 DASUPW010000000
VERSION     DASUPW010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1456443"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1718
                     /locus_tag="VFY44_06715"
     CDS             <1..1718
                     /locus_tag="VFY44_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5910169.1"
                     /translation="ERKRRSGYAFPTDSEWQVEFEGAFPYRETPDQMDAIEAVRADME
                     EGRPMDRLICGDVGYGKTEVALRAAFKAAADAKQVMFLVPTTVLAQQHFGTFAERLRD
                     YPFRIEVASRFQSAAQTRQVLKDFQDGKVDVLVGTHRLLSRDVRPKDLGLLIVDEEQR
                     FGVKQKELLRQLRLRVDVISLSATPIPRTLQMSLAGLRDISVIETPPEGRRPVRTFVG
                     EYDEELVKQAIKREVDRKGQAYFLHNRVDSIEETAERVRAMVPEARVIVAHGQMEEKQ
                     LEQVMLTFLRGEADVLVCTTIIESGLDISSANTLIVERADDLGLSQLYQIRGRVGRSS
                     ERAYAYLLYPSTAALTEEASARLATLSDYTELGSGFKIAMRDLEIRGAGNLLGDEQSG
                     HVAAVGFELYVGMLDEAVAALAADSAEEAPEPVRVDLPVDAFVPGDYVPYEAAKIEIH
                     RRVSGAKEIADLIVLREELADRFGPLPEPLDNLIRLQDARIKLGRAGARTVDFAGGKL
                     AVAPIDIDSRRVKILRARIPEAIYESGRSTVRIRVPDDAAARFPAVVTAAEAILEAAS
                     APDED"
     gene            complement(1666..>2133)
                     /locus_tag="VFY44_06720"
     CDS             complement(1666..>2133)
                     /locus_tag="VFY44_06720"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5910170.1"
                     /translation="RPGCGREGDAALADALGTGVVPGWTTFTGALEHATPGPWGTHLF
                     LAGDPAELVGWGGFKGPPVDGVVELGYEIAEARQGRGLATAAVRAMLAQAFGAPEVQE
                     VIAHTLAERNASNRVLEKAGFAFAREAEEDGQAVWRFSLRREPTPPPGSPPRP"
BASE COUNT          310 a          787 c          755 g          281 t
ORIGIN      
        1 cggagcgcaa gcgccgctcg ggctacgcct tcccgaccga ctcggagtgg caggtggagt
       61 tcgagggcgc gttcccctac cgcgagacgc cggaccagat ggacgccatc gaagcggtgc
      121 gcgccgacat ggaggagggc cgcccgatgg accgcctgat ctgcggcgac gtgggctacg
      181 gcaagaccga ggtggcgctg cgggccgcct tcaaggccgc ggccgacgcc aagcaggtca
      241 tgttcctggt gcccaccacg gtcctggccc agcagcactt cggcacgttc gccgagcgcc
      301 tgcgcgacta cccgttccgg atcgaggtgg cctcgcgctt ccagagtgcg gcccagaccc
      361 gccaggtgct caaggacttc caggacggca aggtcgacgt gctggtgggc acccaccggc
      421 tgctcagccg cgacgtccgg cccaaggacc tggggctgct gatcgtggac gaggagcagc
      481 gcttcggcgt caagcagaag gagctgctgc gccagctgcg cctgcgcgtg gacgtgatca
      541 gcctctcagc caccccgatc ccgcgcacgc tccagatgtc gctggccggg ctgcgcgaca
      601 tctcggtcat cgagaccccg cccgaaggcc ggcggcccgt gcgcaccttc gtgggcgagt
      661 acgacgagga gctggtcaag caggccatca agcgcgaggt cgaccgcaag ggccaggcgt
      721 acttcctgca caaccgcgtc gactcgatcg aggagacggc cgagcgggtg cgggccatgg
      781 tccccgaggc gcgcgtgatc gtcgcccacg gccagatgga ggagaagcag ctggagcagg
      841 tcatgctgac cttcctgcgc ggcgaggccg acgtgctggt gtgcaccacg atcatcgagt
      901 cgggcctgga catctcctcg gccaacacgc tgatcgtgga gcgcgccgac gacctcggcc
      961 tgtcccagct ctaccagatc agggggaggg tgggccggtc ctccgagcgg gcctacgcct
     1021 acctgctcta cccgtcgacg gcggccctga cggaggaggc cagcgcccgg ctggccacgc
     1081 tgtccgacta caccgagctg ggctcgggct tcaagatcgc gatgcgcgac ctggagatcc
     1141 gcggcgcggg caacctgctg ggcgacgagc agtcgggcca cgtggccgcg gtcggcttcg
     1201 agctgtacgt gggcatgctg gacgaggccg tggccgccct ggccgccgac tcggccgagg
     1261 aggcccccga gcccgtgcgc gtcgacctgc cggtcgacgc cttcgtgccc ggcgactacg
     1321 tgccctacga ggcggccaag atcgagatcc accggcgcgt gtcgggcgcg aaggagatcg
     1381 ccgacctgat cgtgctgcgc gaggagctgg cggaccgctt cggcccgctg cccgagccgc
     1441 tggacaacct gatccggctc caggacgcgc ggatcaagct cggccgcgcc ggcgcccgga
     1501 cggtcgactt cgccggcggc aagctggccg tggcgcccat cgacatcgac tcgcgccggg
     1561 taaagatcct gcgcgcgcgg atccccgagg ccatctacga gtccggtcgc agcaccgtcc
     1621 ggatccgcgt ccctgacgac gccgcggccc gcttcccggc cgtggtcacg gccgcggagg
     1681 cgatcctgga ggcggcgtcg gctcccgacg aagactgaag cgccagaccg cctggccgtc
     1741 ctcctccgcc tcgcgcgcga acgcgaaccc cgccttctcc agcacgcggt tcgacgcgtt
     1801 gcgctcggcc agggtgtggg cgatcacctc ctgcacctcg ggcgcgccga aggcctgcgc
     1861 gagcatcgcc cggaccgccg cggtggccag gccgcgccct tgccgggcct cggcgatctc
     1921 gtagcccagc tccaccacgc cgtccaccgg cggtcccttg aagccgcccc accccaccag
     1981 ctcggccggg tcgccggcca ggaacaggtg ggtgccccag gggcccggcg tcgcgtgctc
     2041 gagcgccccg gtgaacgtgg tccagccggg gaccaccccg gttcccaagg cgtcggcgag
     2101 cgcggcatcg ccttcccggc cgcatccagg acg
//
LOCUS       DASUPW010000298         2071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_2011154, whole genome shotgun sequence.
ACCESSION   DASUPW010000298 DASUPW010000000
VERSION     DASUPW010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2011154"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..1502
                     /locus_tag="VFY44_06725"
     CDS             72..1502
                     /locus_tag="VFY44_06725"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5910171.1"
                     /translation="MTDQPPEDAPAAPAGARPRGERARTMGRGLKVGAQATARGAAGA
                     ARATSRASRFTMRQAQRASEAEGAGESGLSRLIQMHFFNTAGDTAVAISLAGSLFFQV
                     PSGEARGQVALFLGLTMLPFAIVAPLLGPFLDRFAHGRRWAIGATMAVRAFLCWGLAT
                     SVTGDSPWIFAAALGVLVSSKAYGVTRAAAVPRLLPRDFTLVRANGRVSMAGTIGATV
                     SAPLAALCSMAGPEWSLRFAVALFIVATVCAIRLPDKVDSSTGEQELVLRGSVDSPTG
                     KSRSKVRIPPAVAFALRANCGPRWLSGFLLMFMAFLLRENPPESGLRAEVLIGLVIGA
                     AGAGNALGILLASLLKRINPGVTVVLALLAAAGSTLVCALFYGVVTLVLLGLTAGLAQ
                     SLAKFCLDASIQRDVPTRVQASAFARSDTTLQLAWVIGGFVGIALPLEPARLGLSVAV
                     AVLTAWAVFVLGTRSRGTPSTTTAAG"
     gene            complement(1720..1908)
                     /locus_tag="VFY44_06730"
                     /pseudo
     CDS             complement(1720..1908)
                     /locus_tag="VFY44_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006830093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
BASE COUNT          229 a          778 c          728 g          336 t
ORIGIN      
        1 aatcctcagg ttccctgtca ggccggggcg cccaacgtct cgccgtgggt gccattgggc
       61 aggatggagc catgaccgac cagccgcccg aggacgcgcc agcggcgccg gccggcgccc
      121 gtccgcgcgg cgagcgggcg cggacgatgg gccgcgggct gaaggtcggc gcacaggcga
      181 cggcgcgcgg cgcggcggga gcggcccggg cgacgagccg ggccagccgg ttcaccatgc
      241 gccaggcaca gcgcgcctcc gaggcggagg gcgcgggcga gtccgggctc tcgcgcctga
      301 tccagatgca cttcttcaac accgccgggg acaccgccgt cgcgatctcg ctcgccggct
      361 ccctcttctt ccaggtcccg tcgggggagg cacggggaca ggtcgccctg ttcctgggcc
      421 tgaccatgct gccgttcgcc atcgtggcgc cgctgctcgg cccgttcctg gatcgtttcg
      481 cccacggccg ccgctgggcc atcggcgcca ccatggcggt gcgggccttc ctctgctggg
      541 gcctggcgac gtcggtgacc ggcgactcgc cgtggatctt cgccgcggcg ctcggcgtcc
      601 tggtctcctc caaggcgtac ggcgtgaccc gcgccgctgc ggtcccccgg ctgctgcccc
      661 gtgacttcac cctggtccgc gccaacggtc gtgtctcgat ggccggcacc atcggggcca
      721 ccgtctcggc gccgttggcg gcgctctgct cgatggccgg ccccgagtgg tcgctgcgct
      781 tcgccgtcgc gctcttcatc gtcgccaccg tctgcgccat ccggttgccc gacaaggtgg
      841 actcctccac gggtgagcag gagctggtgc tccgcggttc ggtcgactcc cccaccggga
      901 agagccggtc gaaggtgcgg atcccgccgg cggtggcgtt cgcgctccga gccaactgcg
      961 gtccgcgctg gctgtcgggg ttcctgctca tgttcatggc cttcctgctg cgcgagaacc
     1021 cgcccgagtc cggcctgcgg gccgaggtcc tgatcggcct ggtgatcggt gctgccggcg
     1081 ccggcaacgc cctcggcatc ctcctggcct cgctcctcaa gcggatcaac cccggcgtga
     1141 ccgtggtcct ggccctgctc gcggcggccg gctcgacgct ggtctgcgcg ttgttctacg
     1201 gggtggtaac cctcgtgctg ctggggctca cggcagggtt ggcgcagtcg ctggccaagt
     1261 tctgcctcga cgcgagcatc cagcgcgacg tcccgacccg ggtccaggcc agtgccttcg
     1321 cccgcagcga cacgaccctg cagctggcct gggtgatcgg cggcttcgtc gggatcgcgc
     1381 tcccgctcga gccggcccga ctgggactga gtgtggcggt ggccgtcctc acggcgtggg
     1441 cggtcttcgt gctgggcacg cggtcgcggg gcacgccctc gacgaccacc gccgccggct
     1501 agcaggaacg ggtcagagct cgagctcgtc ggccagcgcg cgcagcagct tcgccgtggg
     1561 gcccgcggtc ttcgcctcgg ggtggcgccc gcggcggtag gtctcaccga cgtcgtcgag
     1621 cagtcggatc gtgtcctcca cgatcgtcac catctcctcg ggcgtcttgc gcttcgcgtg
     1681 ggactggttg cgctggaccg acgcgggcgc gtcgaggacg cggacctgca gcgcctggtc
     1741 accgcgacgg ccctgcgcga tgccgaactc cacgcgcgca ccgttcttca gcgtggtcat
     1801 gccctcgggc agcgcgtcgg cgtgcacgta gacgtccgga ccgtcctcct gggacaggaa
     1861 cccgaatccc ttcgcgggat cgaaccactt caccttgccg ctcggcacgc cactgacctt
     1921 ccgtcttccg ctgcggcccc tcgccaccgc gccgccccac gatatgtccc acgccgaccg
     1981 gcggccaacc gggtatgcgc aggaccggat tcagagaagc tcgttcaggg accccgaccg
     2041 gaacccgcgc gggtcgatgc tcgcgtcctc g
//
LOCUS       DASUPW010000299         3550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_763960, whole genome shotgun sequence.
ACCESSION   DASUPW010000299 DASUPW010000000
VERSION     DASUPW010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763960"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VFY44_06735"
     CDS             <1..474
                     /locus_tag="VFY44_06735"
                     /inference="COORDINATES: protein motif:HMM:NF013813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910172.1"
                     /translation="AEDDYPAGSEDGTGTLTSDGRDFAFQFVQVGNPQVSIEVADGLE
                     ELDLPALGAPIERSALFPNRTNVSFWRQTGDAEIRARIFERGVGETMSSGTGATGAAV
                     AAVLRGADSPVTVKLDGGDLEVEVGEDLHVDLTGWAVPVYAGELSDEFVQALRER"
     gene            complement(471..1934)
                     /locus_tag="VFY44_06740"
     CDS             complement(471..1934)
                     /locus_tag="VFY44_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910173.1"
                     /translation="MRSLAGVLALLTLICAFAAGPAGAATLKSDNVRLLGKRPEAAGA
                     IGAKFSPDGDTMYVTAATGLFIYDVSVPAVPKLQGHLTLPHFENEDVDVGKDIVVISN
                     DPSFSTVGALYVIDVKDPKKPALKSVTPTQLPASQLNDLLGVKGTNNGHIANCIQDCR
                     YLWTTGTEEGIAVYDLADKSAPKFLGTFTMPVPKLRAGEPATEPGFTHDVFVDKSGIA
                     WITGEDGTFGYRTTGDPLKPQLVFRSDENVTNTGNSGPGVPGDANSQPLDFLHHNSIR
                     TGLTARRKGPASVGRSTPGHGGTGDVMAVTEEDYLRPGCDGQGSLQTWQITGRRNSDG
                     TRKLELLDLWTTELNELLSLRGRSPATANCSAHWFDVDKGLVAQGWYDQGVRFLDISD
                     PRKIRQVGYYATAGSFWAAYFAPSDPKREVVYGIDTAGGIDVIRIDRSKKALKTVQAP
                     TKGLAKAPAARYQASKKYGMVCSLPGQQLLRRAGIKN"
     gene            1989..3152
                     /locus_tag="VFY44_06745"
     CDS             1989..3152
                     /locus_tag="VFY44_06745"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: protein motif:HMM:NF006756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HEX5910174.1"
                     /translation="MKTSERLDRLPPYLFAELSRKIDAKKAEGVDVISLGIGDPDMPT
                     PDLVPDALAAAAHDPGTHQYPSNRGRAELRDAFARFYDRRFGVQLDTDTEIIPALGAK
                     ECVFNVNLAFLDPGTCALAADPGYPVYTGGPLLTGADAVLMPLVPELGFVPDLDAISA
                     QDAERARLMFLNYPNNPTGAVVPDGFFEKVVEYARANDILVVHDASYTETTFDGYVAP
                     SFLETPGAKEVGVEIFSLSKGYNMTGWRIGAFVGNAEACEAYWKLKTNIDSGMFEAVQ
                     LAGAAALDDGDHIPKEMSAVYQRRRDLVVGALRDVGVDVPTPKATIYVWAPVPEGHTS
                     ASYCELVLEESGVVVSPGGAYGESGEGFFRISLTVEDERLTEAMERIRGSLGG"
     gene            complement(3149..>3550)
                     /locus_tag="VFY44_06750"
     CDS             complement(3149..>3550)
                     /locus_tag="VFY44_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910175.1"
                     /translation="GKLKMKMLPDAGYLLAYVDDVAAGIVQALDSGEVGESYVLGGEC
                     VTMGELVDRAAAAAGRKGPRATMPTPLIKASAPLGRFVGPMMGFPPNLRELIRSSDGV
                     TYYAHDDKARRQLGYVSRPLDQGLRETIDSY"
BASE COUNT          497 a         1218 c         1292 g          543 t
ORIGIN      
        1 gccgaggacg actacccggc cgggagcgag gacggcaccg gcacgctcac ctcggacggc
       61 cgcgacttcg ccttccagtt cgtgcaggtg ggcaacccgc aggtctccat cgaggtcgcc
      121 gacggcctcg aggagctcga cctaccggcc ctgggcgcgc cgatcgagcg cagcgcgctg
      181 ttccccaacc gaaccaacgt ctcgttctgg cgccagaccg gcgacgcgga gatccgcgcg
      241 cgcatcttcg agcgcggggt gggggagacg atgtcgtccg gcaccggcgc caccggcgcc
      301 gccgtcgcgg cggtcctgcg tggcgcggac agcccggtga ccgtgaagct ggacggcggc
      361 gacctcgagg tcgaggtggg cgaggacctg cacgtggacc tcaccggctg ggccgtgccg
      421 gtctacgccg gcgagctcag cgacgagttc gtgcaggccc tgcgcgagcg ctagttcttg
      481 atgcccgcgc ggcgcagcag ctgctggccg ggaagcgagc agaccatgcc gtacttcttg
      541 ctggcctggt agcgggccgc gggcgccttg gccaggccct tggtgggggc ctggacggtc
      601 ttcagggcct tcttcgagcg gtcgatccgg atcacgtcga tcccgcccgc cgtgtcgatg
      661 ccgtagacca cctcgcgctt ggggtccgac ggcgcgaagt acgccgccca gaagcttccc
      721 gcggtcgcgt agtagcccac ctggcggatc ttgcgcgggt cggagatgtc caggaagcgc
      781 acgccctggt cgtaccagcc ctgggcgacc aggcccttgt cgacgtcgaa ccagtgggcc
      841 gagcagttgg cggtcgccgg cgagcgcccg cgcaggctca gcagctcgtt cagctcggtg
      901 gtccacaggt ccagcagctc cagcttgcgg gtgccgtcgg agttccggcg gccggtgatc
      961 tgccacgtct ggagcgagcc ctgcccgtcg cagccggggc gcaggtagtc ctcctcggtg
     1021 acggccatga cgtcgccggt gccgccgtgg ccgggggtcg agcgcccgac gctggccggc
     1081 cccttgcggc gcgcggtcag cccggtgcgg atggagttgt ggtgcaggaa gtccagcggc
     1141 tggctgttgg cgtcgccggg gacccccggg cccgagttgc cggtgttggt cacgttctcg
     1201 tcggagcgga acaccagctg cggcttgagc gggtcgcccg tcgtgcggta gccgaacgtg
     1261 ccgtcctcgc cggtgatcca ggcgatcccg gacttgtcca cgaacacgtc gtgcgtgaag
     1321 ccgggctcgg tcgcgggctc gccggcgcgg agcttgggca cgggcatggt gaacgtgccc
     1381 aggaacttcg gcgcgctctt gtccgccagg tcgtagacgg cgatgccctc ctcggtgccg
     1441 gtggtccaga ggtagcggca gtcctggatg cagttggcga tgtggccgtt gttcgtgccc
     1501 ttgactccga gcaggtcgtt cagctggctc gcgggcagct gggtgggcgt cacggacttc
     1561 agcgcaggct tcttcgggtc cttgacgtcg atcacgtaca gcgcgcccac ggtgctgaag
     1621 ctggggtcgt tgctgatgac cacgatgtcc ttgcccacgt cgacgtcctc gttctcgaag
     1681 tgggggagcg tcaggtggcc ctggagcttg ggcacggcgg ggaccgacac gtcgtagatg
     1741 aagagcccgg tggcggcggt cacgtacatc gtgtcgccgt cgggggagaa cttggccccg
     1801 atcgcgccgg cggcctcggg ccgcttgccc aggagccgga cgttgtcgga tttcagcgtc
     1861 gcagcgccgg cggggccggc ggcgaacgca cagatcaggg tgaggagggc caggacccct
     1921 gccagggaac gcatggccgg cgttatcccc acgatcggcg ccctccaatc atccatagag
     1981 tggcccgcat gaagacgagt gagcgcctgg accgcctgcc cccgtacctg ttcgccgagc
     2041 tgtcgcgcaa gatcgacgcc aagaaggccg agggcgtcga cgtgatcagc ctcgggatcg
     2101 gcgatcccga catgcccacg ccggacctgg tgccggacgc cctggcggcg gccgcacacg
     2161 acccgggcac ccaccagtac ccgtccaacc gcggccgcgc cgagctgcgc gacgccttcg
     2221 cccgcttcta cgaccgccgc ttcggggtcc agctggacac cgacaccgag atcatccccg
     2281 cgctgggggc caaggagtgc gtgttcaacg tgaacctggc cttcctggac ccgggcacgt
     2341 gcgcgctggc cgccgacccc ggctacccgg tctacacggg cgggccgctg ctcaccggcg
     2401 ccgacgcggt gctgatgccg ctggtcccgg agctgggctt cgtgcccgac ctggacgcca
     2461 tctcggccca ggacgccgag cgcgcccggc tgatgttcct gaactacccg aacaacccca
     2521 ccggcgccgt ggtgccggac gggttcttcg agaaggtcgt ggagtacgcg cgcgccaacg
     2581 acatcctcgt ggtgcacgac gcctcgtaca ccgagaccac cttcgacggc tacgtggcgc
     2641 ccagcttcct ggagacgccc ggggccaagg aggtcggcgt cgagatcttc tcgctgtcca
     2701 agggctacaa catgaccggc tggcggatcg gggcgttcgt gggcaacgcc gaggcgtgcg
     2761 aggcctactg gaagctcaag accaacatcg actcgggcat gttcgaggcg gtccagctgg
     2821 ccggggccgc cgcgctggac gacggcgacc acatcccgaa ggagatgagc gcggtctacc
     2881 agcgccggcg cgacctggtg gtcggcgccc tgcgcgacgt cggcgtggac gtgcccacgc
     2941 ccaaagcgac gatctacgtg tgggcgcccg tgcccgaggg gcacacctcg gcgtcctact
     3001 gcgagctggt gctggaggag tcgggcgtgg tggtctcgcc cggcggcgcc tacggcgaga
     3061 gcggcgaggg cttcttccgc atctccctga ccgtcgagga cgagcgcctg accgaggcga
     3121 tggagcgcat ccgcgggagc ctgggcggct aataggagtc gatcgtctcg cgcagcccct
     3181 ggtccagggg gcgcgagacg tagccaagct gcctgcgcgc cttgtcgtcg tgggcgtagt
     3241 aggtcactcc gtcggacgag cggatcagct cgcgcaggtt gggcgggaag cccatcatcg
     3301 ggccgacgaa gcgccccagc ggggcggagg ccttgattag cggcgtgggc atcgtcgcgc
     3361 ggggtccctt gcggccggcg gccgcggcgg cacggtccac cagctcgccc atggtcacgc
     3421 actcaccgcc gagcacatac gactctccga cctcgccgga gtccagggcc tgcacgatgc
     3481 cggcggccac gtcgtccacg taggccagca ggtagccggc gtcgggcagc atcttcatct
     3541 tcagcttgcc
//
LOCUS       DASUPW010000300         2712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_764118, whole genome shotgun sequence.
ACCESSION   DASUPW010000300 DASUPW010000000
VERSION     DASUPW010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764118"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1503)
                     /locus_tag="VFY44_06755"
     CDS             complement(49..1503)
                     /locus_tag="VFY44_06755"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX5910176.1"
                     /translation="MAGEAITQRSGLALGRAIRAGELSSRQVVEEHIGLLRRLEPALG
                     AIAVDRYDAALEEADAADARIAAAADGEELPPYLGVPCTVKESIGFEGMPNTAGVVAR
                     RGTLAPQSAPTTQRLLDLGAIPLGVTNTSELTMWIESVNRVYGRTNNAYDAGRTAGGS
                     SGGEGAVVGSGGAPIGLGSDIGGSIRLPAFFNGVFGHKCTAHLVPNSGQWPEAIGEPT
                     RMLAVGPIVRRAEDLMPVLSAIAGPDGTDPDTREVALGDPADVELSGLPVIIGQDITP
                     PKIARELLDVRERAAGALAAAGASVRTESLKSIRRAMEYYLATLASGEVEEVWIQVGG
                     TPGTRAPLRRIIGDAVRGRGDHTVALAILLAVERMGDRIPDKRNAKAVEAGRALAREV
                     EDTIGDGVLLHPPFGRVAPKHGRTVGKPWVILPAAIFNLMGLPATEIPLGLNPEGLPL
                     GVQAVAGRDRDHVSIAVALELERVFGGWVPPHPA"
     gene            1769..2431
                     /locus_tag="VFY44_06760"
     CDS             1769..2431
                     /locus_tag="VFY44_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910177.1"
                     /translation="MYACGAHAVLGGLAGAWRYRVLKGAPPRAEVIAPSERRVPGVLT
                     RRGHWPHSTFHGIPTLTVPATLVDIAARLTVADLARAAHESGVLYRTTPRQVDAVLRR
                     RPATKGAANLRLVLSGDERVALSRLESTFLALLRAERLPLPVTNRPAGGKRVDCRWPE
                     QRLTVELDSFAFHNSRHSWQQDRDRERQAYARGDEFRRYTWADVFETPARMLAELRAL
                     LP"
     gene            complement(2428..>2712)
                     /locus_tag="VFY44_06765"
     CDS             complement(2428..>2712)
                     /locus_tag="VFY44_06765"
                     /inference="COORDINATES: protein motif:HMM:NF018351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-related domain-containing protein"
                     /protein_id="HEX5910178.1"
                     /translation="AEDDDFVQARTLYNDVMEDVNREALVEGIVGHASKPEVTSAMHE
                     RVIAYWANVDADLGAKVAAGLGKGNGQGANGSGPASPEATELLAERANRA"
BASE COUNT          319 a         1039 c          988 g          366 t
ORIGIN      
        1 cctcgtaacg ggccatgctg gtggtacgcc gcccggcgcc gccgcggatc aggcggggtg
       61 gggcggcacc cagccgccga acacgcgctc cagctccagc gccacggcga tcgacacgtg
      121 gtcgcggtcg cggccggcca cggcctgcac gcccagcggg agcccctcgg ggttcagccc
      181 gagcgggatc tcggtggccg gcaggcccat caggttgaag atcgctgcgg gcaggatcac
      241 ccacggtttg cccaccgtcc gcccgtgctt gggcgccacg cgcccgaagg gcgggtgcag
      301 caggaccccg tcgccgatgg tgtcctcgac ctcccgcgcc agcgcgcggc cggcctccac
      361 cgccttggcg ttgcgcttgt ccgggatgcg gtcgcccatc cgctccaccg ccaggaggat
      421 ggccagcgcc accgtgtgat cgccgcgccc gcgcacggcg tcgccgatga tgcggcgcag
      481 cggcgcccgg gtgcccggcg tgccgcccac ctggatccag acctcctcga cctcgcccga
      541 ggccagcgtc gccaggtagt actccatcgc cctgcggatc gacttgagcg actcggtgcg
      601 cacgctggcg cccgccgcgg ccagggcccc ggccgcgcgc tcgcgcacgt ccagcagctc
      661 gcgggcgatc ttcggcggcg tgatgtcctg gccgatgatc accggcagcc cgctgagctc
      721 cacgtcggcc gggtcgccga gggcgacctc gcgggtgtcc gggtcggtgc cgtcggggcc
      781 ggcgatggcg ctgagcacgg gcatcaggtc ctcggcccgg cgcacgatcg gccccacggc
      841 cagcatccgc gtgggctcgc cgatggcctc cggccactgg ccgctgttgg gcaccaggtg
      901 ggccgtgcac ttgtggccga acacgccgtt gaagaacgcg ggcagccgga tcgagccgcc
      961 gatgtcgctg cccaggccga tcggagcgcc gccgctgccc accacggcgc cctcgccccc
     1021 ggacgacccg cccgcggtgc ggccggcgtc gtaggcgttg ttggtgcgcc cgtagacgcg
     1081 gttcaccgac tcgatccaca tggtcagctc cgaggtgttc gtcaccccca gcgggatcgc
     1141 gcccaggtcc agcaggcgct gtgtcgtggg cgccgactgg ggggccagcg tgcccctgcg
     1201 ggccaccacc ccggcggtgt tgggcatgcc ctcgaacccg atcgactcct tcaccgtgca
     1261 gggcaccccc aggtacgggg gcagctcctc gccgtcggcc gcggcggcga tgcgggcgtc
     1321 ggccgcgtcg gcctcctcca gcgcggcgtc gtagcggtcg accgcgatgg cccccagggc
     1381 gggctccagg cgccgcagca ggccgatgtg ctcctcgacc acctgccgcg agctgagctc
     1441 accggcccgg atggcccggc cgagcgcgag cccggaacgc tgggtgatcg cctcccccgc
     1501 cacgggcgcc agcctacccg gggggcgaag ctcggtgccg cgctgacttg acccggttat
     1561 ggcgggcaaa ctcagcgcag ccgaggcgat gtcccgcctg ggagcccgcg cgttcggtgt
     1621 ggtcacgcgg cggcagcttc tcgcggcggg cctcaccaga caccagatcg acggacgagt
     1681 tgcggcgggt tcgctgatac cggtgcaccg cggcgtctac cgcgtcggtc atgcggcgcc
     1741 cagtaccgac gcctcctacc ttgccgccgt gtacgcctgc ggcgcgcacg cggttctcgg
     1801 aggcctggcc ggcgcgtggc gctaccgggt gctgaagggg gcgccgccgc gcgcggaagt
     1861 catcgcgccg agtgagcgcc gcgtccccgg cgtcctgacc cgccgtggcc actggccgca
     1921 ctccaccttc cacggcatcc ccaccctgac cgtgccggcg acgctggtgg acatcgcggc
     1981 gcggctgacc gtggcggacc tggcccgcgc ggcgcacgag tcgggggtgc tctaccggac
     2041 cacgccacgc caggtcgacg ccgtgctgcg aaggcggccg gccaccaagg gcgcggccaa
     2101 cctgcggctg gtgctcagcg gcgacgagag ggtcgcgctg agccggctgg agtcgacctt
     2161 cctggcgctg ctgcgggccg agcgcctccc cctcccggtg acgaaccggc ccgccggcgg
     2221 caagcgcgtg gactgccgct ggccggagca gcggctgacc gtcgagctgg acagcttcgc
     2281 cttccacaac tcgcggcaca gctggcagca ggaccgcgac cgcgaacggc aggcgtacgc
     2341 acgcggggac gagttccggc gctacacctg ggcggacgtc ttcgagacgc ccgcccggat
     2401 gctggccgag ctgcgggccc tgctgcccta ggcccggttc gcgcgctcgg ccaggagctc
     2461 ggtggcctcg ggtgacgccg ggccggagcc gttggcgccc tggccgttgc ccttgcccag
     2521 gccggccgcg accttggcgc ccaggtcggc gtccacgttg gcccagtagg cgatcacgcg
     2581 ctcgtgcatg gcgctggtga cctcgggctt gctggcgtga cccacgatgc cctcgaccag
     2641 cgcctcgcgg ttcacgtctt ccatgacgtc gttgtagagc gtgcgcgcct ggacgaagtc
     2701 gtcgtcctcg gc
//
LOCUS       DASUPW010000301         3301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_764391, whole genome shotgun sequence.
ACCESSION   DASUPW010000301 DASUPW010000000
VERSION     DASUPW010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764391"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VFY44_06770"
     CDS             <1..307
                     /locus_tag="VFY44_06770"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX5910179.1"
                     /translation="REAEAPTAGPATTSVVFWGFNDESPDALVSVLGELSSRDINLTR
                     IESRPRRVRLGHYMFFADLDGSTADPHVAEALAALEARVHELRLLGSYPSGPGRAPR"
     gene            440..934
                     /locus_tag="VFY44_06775"
     CDS             440..934
                     /locus_tag="VFY44_06775"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX5910180.1"
                     /translation="MLNASYEPINVCTVRRAAVLVLKERAEILERSEWSLHAESMTLP
                     RPMVIRLTTYVRIPRDAHRRKITRRAVFARDEWTCQYCGQERGNLTVDHVIPRSKGGD
                     SGWENIVACCAPCNRRKGDRLPRQANMVPRTKPRAPGSTVFIHVAAPVIPKAWEQYLA
                     GTRT"
     gene            937..1377
                     /locus_tag="VFY44_06780"
     CDS             937..1377
                     /locus_tag="VFY44_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910181.1"
                     /translation="MPSVDVEKEQAGAPVTQEDLRTLRRWIVVAGVWAVAATIVALVA
                     LLDDSGRAAERRANETGRRVAATQQRVDDRLARLERRLAATPTTTQVQQLRTQVAQAQ
                     RAASGASTSVTALEKRVDSLESDGGSGTDTPPDDGLPRLRDLFP"
     gene            complement(1640..2116)
                     /locus_tag="VFY44_06785"
     CDS             complement(1640..2116)
                     /locus_tag="VFY44_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910182.1"
                     /translation="MKSFTLALSAALLLAAAPAASAATYKFGADLERADFRDGKAGKA
                     TFKVKTNKKGVASKVTSVKLSGLKSLCLSEDGVKPGATVSGTLGGSISIKKPTSSFNK
                     YSFQKSGLKLGGFAFSVSGTVNKAGTKVMSFRIDGGDTNPQPPSCTARGDGKPKKK"
     gene            complement(2192..>3301)
                     /locus_tag="VFY44_06790"
     CDS             complement(2192..>3301)
                     /locus_tag="VFY44_06790"
                     /inference="COORDINATES: protein motif:HMM:NF015922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEX5910183.1"
                     /translation="SEDDIDIEDLIADQQMVIAITRSGYIKRLPLDTYRKQKRGGVGV
                     TGMDMKDEDYIEHLFVCSTHDYLLFFTNRGKVYRQKVYELPEAGRTARGRALVNVLPL
                     REGERVQSVLSTRDFTEGKYLVFATRQGQIKKTEFLAYNTPIKADGIIAIKVRDDDEL
                     VAVRRTDGEDDIIMIARSGQAARFSEAEARPMGRDTAGVKGMNVSRGDNCVLAMDIAR
                     DDTELLVITENGFGKRTPIADYPVKGRGAMGVKTINITEKKGGVAGALIVREHQDLVF
                     ISREGMVQRTGVKGISQQGRAAQGVTLMNLREDDIVSAVALVMEDEADTSAVVADAAA
                     EELKPIDEIPSDVPVELDPGVAEDPEPAADEDDED"
BASE COUNT          455 a         1188 c         1122 g          536 t
ORIGIN      
        1 ccgggaggcc gaagcgccca ccgcgggccc ggccaccacg tctgtcgtgt tctggggctt
       61 caacgacgag tcccccgacg cgctggtgtc cgttctcggc gagctctcca gccgcgacat
      121 caacctgacc cggatcgagt cccggccgcg gcgggtgcgg ctcgggcact acatgttctt
      181 cgccgacctg gacgggtcga cggcggaccc gcacgtggcc gaggcgctgg ccgcgctgga
      241 ggcgcgcgtg cacgagcttc ggctcctggg ctcctatccc agcgggccgg gccgggcccc
      301 tcgctagaat cgcgggaccg tgacggcccg ctacgaagcg gttcagacag caccggagac
      361 cgggccagtc ccagtcccta tcggtgcgct gcccgccggc cggagcgggc agcgtggagg
      421 atcgaccggc cgggtcctcg tgctgaacgc gtcctatgag cccatcaacg tctgcaccgt
      481 ccgccgcgcc gccgtgctcg tgctgaagga acgggccgag atcctcgagc ggtcggagtg
      541 gtcgctgcac gccgagtcga tgaccctccc gcgcccgatg gtcatccgcc tgaccacgta
      601 cgtgcggatc ccgcgcgacg cccaccggcg caagatcacc cgccgcgccg tgttcgcccg
      661 cgacgagtgg acctgccagt actgcggcca ggagcgcggc aacctgaccg tcgaccacgt
      721 gatcccgcgc tcgaagggcg gcgactccgg ctgggagaac atcgtcgcgt gctgcgcccc
      781 ctgcaaccgc cgcaagggcg accgtctccc ccgccaggcc aacatggtgc cgcgcaccaa
      841 gcccagggcc cccggcagca cggtgttcat ccacgtggcc gcgccggtca tccccaaggc
      901 gtgggagcag tacctggccg gcaccaggac ctgaccatgc cctccgtgga cgtcgagaag
      961 gaacaggcgg gagcgccggt cacccaggag gacctgcgca cgctgcgccg ctggatcgtc
     1021 gtcgcgggcg tctgggcggt ggccgccacc atcgtcgccc tggtcgcgct gctggacgac
     1081 tcgggccgcg cggccgagcg gcgggccaac gagacggggc ggcgcgtggc ggcgacccag
     1141 cagcgcgtcg acgatcggct ggcccggctc gagcgccgcc tggctgccac ccccaccacg
     1201 acccaggtgc agcagctgcg cacccaggtg gcccaggccc agcgggcggc gtcgggagcg
     1261 tccaccagcg tcacggcgct cgagaagcgc gtcgactcgc tggagagcga cggcggctcc
     1321 gggaccgaca caccgccgga cgacgggctt ccccgcctcc gcgacctctt cccctagaac
     1381 gacgaagggc gccccggatg ggacgccctt cgtacgtctg atctggacca gcaccggagc
     1441 gcgttcacag gcaattggcc tgggccggag ggacaagtcc cggcctgcag ttccggcgga
     1501 tgtccggttg gactccgcta agcggggccc agcatccgtg cctagcggcc ggacacctcc
     1561 agagtcccgt catgtatgtg aatcaagtta ggaccacctc ctttccctgg tgaatcgaaa
     1621 cctagcaagg cgggtggctc tactttttct tgggcttccc gtcgccgcgc gcggtgcagc
     1681 tcggcggctg ggggttggtg tcccctccgt cgatccggaa gctcatgacc ttcgtgccgg
     1741 ccttgttcac ggtcccgctc accgagaagg cgaagccgcc gagcttgagc ccggacttct
     1801 ggaacgagta cttgttgaac gagctcgtcg gcttcttgat cgagatgctg ccgcccaggg
     1861 ttccggacac cgtcgccccc ggcttcacgc cgtcctcgct gaggcacagc gacttcagcc
     1921 cgctgagctt caccgacgtg accttcgacg cgacgccctt cttgttggtc ttgaccttga
     1981 acgtggcctt gccggccttg ccgtccctga agtcggcgcg ttcgaggtcg gcgccgaact
     2041 tgtaggtggc ggccgaggcg gcgggcgccg cggccagcag cagcgccgcg ctcagcgcca
     2101 gggtgaagct cttcatgtgt ctcctcgcgg tagtggcccc tgtcggggcc accctaggag
     2161 cgaggccact cccccgccac tagcaggggg gctagtcctc gtcgtcctcg tccgctgccg
     2221 gctcggggtc ctcggcgacc ccgggatcga gctccacggg gacgtcgctc gggatctcgt
     2281 cgatcggctt gagctcctcc gcggcggcgt ccgcgacgac cgccgacgtg tcggcctcgt
     2341 cctccatgac cagggcgacc gcgctgacga tgtcgtcctc gcgcaggttc atgagcgtca
     2401 cgccctgggc cgcgcggccc tgctgggaga tgcccttgac gccggtgcgc tggaccatcc
     2461 cctcgcgtga gatgaagacc aggtcctggt gctcgcgcac gatcagcgcg ccggccaccc
     2521 cgcccttctt ctcggtgatg ttgatggtct tgacgcccat cgcgccgcgg cccttgaccg
     2581 ggtagtccgc gatcggcgtg cgcttgccga agccgttctc ggtgatcacc aggagctcgg
     2641 tgtcgtcgcg ggcgatgtcc atggccagca cgcagttgtc cccgcgcgag acgttcatgc
     2701 ccttcacacc ggcggtgtcg cggcccatcg gccgcgcctc ggcctcggag aaccgcgcgg
     2761 cctggcccga gcgggcgatc atgatgatgt cgtcctcgcc gtcggtacgg cggacggcca
     2821 ccagctcgtc gtcgtcgcgg accttgatgg cgatgatgcc gtcggccttg atcggcgtgt
     2881 tgtaggccag gaactccgtc ttcttgatct ggccctggcg ggtggcgaag accaggtact
     2941 tgccctcggt gaagtcgcgc gtggacagca cggactgcac ccgctcgccc tcgcgcagcg
     3001 gaagcacgtt gaccagcgcg cgcccgcgcg ccgtccggcc ggcctcgggc agctcgtaca
     3061 ccttctggcg gtagaccttg ccgcggttgg tgaagaacag gaggtagtca tgcgtcgagc
     3121 acacgaagag gtgctcgatg tagtcctcgt ccttcatgtc catgccggtc acgccgacgc
     3181 cgccgcgctt ctgcttgcgg taggtgtcca gcgggagccg cttgatgtag cccgagcgcg
     3241 tgatcgcgat gaccatctgc tggtcggcga tcaggtcctc gatgtcgatg tcgtcctcgg
     3301 a
//
LOCUS       DASUPW010000302         3589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_626203, whole genome shotgun sequence.
ACCESSION   DASUPW010000302 DASUPW010000000
VERSION     DASUPW010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626203"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VFY44_06795"
     CDS             <1..476
                     /locus_tag="VFY44_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX5910184.1"
                     /translation="LDEDEVANVRHAAELHDVGKVAVPDDILSKPGPLDDEEFAFIRR
                     HTLIGERIISAAPALSEVARLVRSSHERWDGTGYPDGLAGEEIPLASRIVFAADAFDA
                     MTSTRPYNTPRSADEAIRELRACAGTQFDPVVVEAFRAAWDEAQATSRSLPVSTS"
     gene            complement(437..928)
                     /locus_tag="VFY44_06800"
     CDS             complement(437..928)
                     /locus_tag="VFY44_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX5910185.1"
                     /translation="MSTTDELLKNNEDYAAGFDKGDLPLPPGKKVAIVACMDARLHPT
                     SALGLEEGDAHVIRNAGGVITDDEIRSLAISQNLLGTEEIILIHHTDCGMLTFTDEQF
                     KSQLEESTGQRPDWDSEAFPDLEANVRSSLQKVNDSPFIPNKESVRGFVYEVETGRLR
                     EVA"
     gene            1040..1306
                     /gene="ffs"
                     /locus_tag="VFY44_06805"
     ncRNA           1040..1306
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFY44_06805"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            1324..1914
                     /locus_tag="VFY44_06810"
     CDS             1324..1914
                     /locus_tag="VFY44_06810"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5910186.1"
                     /translation="MADPAPSTSSPFVQGAQVLLAMVAVMWALEIADVALDHRLDDYG
                     IEPRDVDALPGVVSSPFLHAGFGHLIGNTIPFVAMGLAIALAGAKRLISVTVIVGLIA
                     GLGTWLTAGANTETIGASGLVFGYATYLMTRFFFSRRAPDLLLGIGVLVLWGASLLAS
                     LAPTPGVSWQAHLFGAVGGVVAARVVGSDSAGQRAP"
     gene            complement(1891..2433)
                     /locus_tag="VFY44_06815"
     CDS             complement(1891..2433)
                     /locus_tag="VFY44_06815"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX5910187.1"
                     /translation="MSEALTRLEAVTLGLLVDQPTHPYALRARLAPGLPPEGRPNDGV
                     VRPLLARLEQRGLLRHQRDRVDGRERKVFHATAAGRRAFAAWLDSDAHEGRELGADLF
                     LDQPFLKLLFAGHMSPGRQQAKLRSIRDAAAGRGQALERLRDDGRALGTVGAALLELG
                     MEREQATVQVADRLRRSLAG"
     gene            complement(2430..3098)
                     /locus_tag="VFY44_06820"
     CDS             complement(2430..3098)
                     /locus_tag="VFY44_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5910188.1"
                     /translation="MSLIIETTGPVSILRMDDGENRFNPDTLAALDEAFDELERADGP
                     RAAVLTGTGKFFSNGLDLEWMSGAGQGEPEQVVAGTQALLAKVLLAPMPVLAAVNGHA
                     FAAGAMLALACDERVMRADRGYFCLPEVDIGLPFTAGMAALITARLAPAAAHRLMVTG
                     DRVGGGEAENLGIVGATAAEDAVLATTVERAGQLAGKAGPTCAAIKAGLYAPVGELLR
                     APAA"
     gene            3144..>3589
                     /locus_tag="VFY44_06825"
     CDS             3144..>3589
                     /locus_tag="VFY44_06825"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5910189.1"
                     /translation="MLSLYRRHRPRTFDQVVGQESVVRTLRNAIELGKVHHAYLFVGS
                     RGTGKTSMAKLLACAMNAEGGPRTDFSPDDPACKAIMNGTSLDVVEMDAASNNSVDDI
                     RELRENVSLSPMGGSRRVYILDEAHMLSTAAWNAFLKTLEEPPAHVV"
BASE COUNT          484 a         1278 c         1298 g          529 t
ORIGIN      
        1 cgctggacga ggacgaggtg gccaacgtgc gccacgccgc cgagctgcac gacgtcggga
       61 aggtggccgt ccccgacgac atcctgtcca agccggggcc gctggacgac gaggagttcg
      121 ccttcatccg ccggcacacg ctgatcggcg agcggatcat ctccgccgcg cccgccctca
      181 gcgaggtggc ccgcctggtc cggtcgagcc acgagcgctg ggacggcacg ggataccccg
      241 acggcctggc cggcgaggag atcccgctgg cgtcgcggat cgtgttcgcc gccgacgcgt
      301 tcgacgcgat gacctccacc cgcccgtaca acacgccgcg atcggccgac gaggccatcc
      361 gcgagctacg cgcgtgcgcc gggacccagt tcgacccggt cgtggtggag gcgttccgcg
      421 ccgcctggga tgaggctcag gccacctcgc gcagcctgcc ggtctcgacc tcgtagacga
      481 agccgcgcac cgactccttg ttggggatga acgggctgtc gttgaccttc tgcagcgacg
      541 agcgcacgtt ggcctcgagg tcggggaacg cctcgctgtc ccagtccggg cgctggcccg
      601 tggactcctc gagctggctc ttgaactgct cgtcggtgaa cgtgagcatc ccgcagtcgg
      661 tgtgatggat caggatgatc tcctcggtcc cgagcaggtt ctgggagatc gccagggagc
      721 ggatctcgtc gtcggtgatc acgccgccgg cgttgcggat cacgtgcgcg tcgccctcct
      781 cgagcccgag cgcgctggtg gggtgcaggc gggcgtccat gcaggcgacg atcgcgacct
      841 tcttgccggg gggcaggggc aggtcgccct tgtcgaagcc ggcggcgtag tcctcgttgt
      901 tcttcagcag ttcgtcggtg gtgctcatgg gcgctcctgg ctcgggtggt ctgacggggc
      961 cgcgccaatt ctagacaaat ccaatcggaa atataggttt tggcgcgggg gcagccgacc
     1021 ggctaccatc gccaacggac cgtgctaggc ggggatttag cggagcccta cacccgcaat
     1081 ccgctctagc ggggctgaat tcccatccga gggatgaggc cctcggggtc agtacgccgg
     1141 atcgaggcgc tgatggccgg gtcccgcgcg gtgagcgatc gcgaaccagg tcaggtccgg
     1201 aaggaagcag ccttaagcga agccgttcat gcgccgcgga ccagcctggc ttgagccgag
     1261 acacggcggg cacgcccggg gacccttcac ggagatgggt gcacggtcca tatattccgc
     1321 tgaatggccg atcccgcccc cagcacgtcc tcgcccttcg tccagggcgc ccaggtcctg
     1381 ctggccatgg tggccgtcat gtgggccctg gagatcgccg acgtcgcgct ggaccaccgg
     1441 ttggacgact acggcatcga gccgcgggac gtggatgcgc tgccgggggt ggtcagctcg
     1501 ccgttccttc acgcgggctt cggccacctg atcggcaaca ccatcccgtt cgtggccatg
     1561 gggctggcga tcgcgctggc cggggccaag cgcctgatca gcgtgaccgt gatcgtcggc
     1621 ctgatcgcgg gcctgggcac gtggctgacg gccggggcca acaccgagac gatcggggcc
     1681 agcgggctgg tgttcggcta cgcgacctac ctcatgacgc ggttcttctt cagccgccgg
     1741 gcgccggacc tgctgctggg catcggcgtc ctggtgctgt ggggcgcctc gctgctggcc
     1801 agcctggcgc cgacccccgg cgtgtcctgg caggcgcacc tgttcggcgc ggtcggcggc
     1861 gtcgtggccg cccgcgtggt gggcagcgac tcagccggcc agcgagcgcc gtagccggtc
     1921 ggccacctgg acggtggcct gctcgcgctc catgcccagc tccagcagcg ccgcgcccac
     1981 cgtgccgagt gcgcgcccgt cgtcgcgcag ccgctccagg gcctggccgc ggccggcggc
     2041 ggcatcgcgg atggaccgga gcttggcctg ctggcggccc ggggacatgt gcccggcgaa
     2101 cagcagcttc aggaacggct ggtccaggaa caggtcggcg cccagctcgc ggccctcgtg
     2161 ggcgtcgctg tccagccagg cggcgaacgc gcgccggccc gcggccgtgg cgtggaagac
     2221 cttgcgctcg cggccgtcga cccggtcgcg ctggtggcgc agcaggccgc gctgctcgag
     2281 ccgggccagc aggggccgga cgacgccgtc gttgggccgg ccctcgggcg ggaggccggg
     2341 ggccagccgg gcgcgcagcg cgtacgggtg ggtgggctgg tcgaccagca gccccagcgt
     2401 gaccgcctcg agcctggtca gcgcctcgct cacgccgccg gcgcccgcag gagctcgcca
     2461 accggggcgt acaggccggc cttgatcgcg gcgcaggtgg ggccggcctt cccggccagc
     2521 tgcccggccc gctcgaccgt cgtggccagc acggcgtcct ccgcggccgt cgcccccacg
     2581 atccccaggt tctcggcctc gccgccgccc acgcggtcgc ccgtgaccat cagccggtgg
     2641 gcggccgccg gggccaggcg cgcggtgatc agggccgcca tgcccgcggt gaacggcagt
     2701 ccgatgtcca cctcgggcag gcagaagtag ccgcggtcgg cgcgcatcac gcgctcgtcg
     2761 caggccaggg ccagcatggc cccggcggcg aaggcgtggc cgttcacggc cgccagcacc
     2821 ggcatcgggg ccagcagcac cttggccagc agcgcctggg tgccggcgac cacctgctcg
     2881 ggctcgccct ggccggcgcc ggacatccac tccaggtcca gcccgttgga gaagaacttc
     2941 cccgttccgg tgagcacggc ggcgcgcggg ccgtcggctc gctcgagctc gtcgaaggcc
     3001 tcgtccagcg ccgcgagggt gtccgggttg aagcgattct cgccgtcgtc catgcgcagg
     3061 atggacaccg ggccggtggt ctcgatgatc aggctcatgc atagagtcta tgcataggcc
     3121 gaccatctag catggggccg ccgatgctct cgctctaccg ccgccaccgc ccccggacct
     3181 tcgaccaggt cgtgggacag gagagcgtgg tccgcaccct gcgcaacgcc atcgagctgg
     3241 gcaaggtgca ccacgcctac ctgttcgtgg gctcgcgcgg gacgggcaag acttctatgg
     3301 ccaagctgct ggcctgcgcc atgaacgcgg agggcggccc gcgcacggac ttctcgcccg
     3361 acgacccggc ctgcaaggcg atcatgaacg gcacctcgct ggacgtggtc gagatggacg
     3421 cggcatccaa caactccgtg gacgacatcc gcgagctgcg cgagaacgtg tcgctgtcgc
     3481 ccatgggcgg ctcgcgccgc gtgtacatcc tggacgaggc ccacatgctc tcgaccgcgg
     3541 cctggaacgc cttcctcaag acgctcgagg agccccccgc gcacgtcgt
//
LOCUS       DASUPW010000303         3466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_350670, whole genome shotgun sequence.
ACCESSION   DASUPW010000303 DASUPW010000000
VERSION     DASUPW010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350670"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..708)
                     /locus_tag="VFY44_06830"
     CDS             complement(163..708)
                     /locus_tag="VFY44_06830"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5910190.1"
                     /translation="MSEPPRVTSSEVVYENRWMRLREDRLERDDGSPGLYSVVEKRPA
                     AVILPVHEDGSVTLVEQWRHPVGERFWELPQGALDGQDQAEPEHIARTELAEETGLRA
                     ASMRRLGRLYFAYGISDQYFDAWLATGLEQGEQALEAEEEGLRVRRFAPGHVDEMIGA
                     GTIADAATVAVFRLHDREKRG"
     gene            complement(705..1862)
                     /locus_tag="VFY44_06835"
     CDS             complement(705..1862)
                     /locus_tag="VFY44_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5910191.1"
                     /translation="MSTTATPGADAYTLPQEFKDFQQTIRELVAGEIAPRAAEIDSTG
                     EYPWDVRRTLAEHDILGLPFPEEYGGTGTGTLMLNVAVEEIAKACASSALILMVQELG
                     TLPIQLHGSEELKQKYLPDCASGEKSPAFCLSEPEAGSDPGSMRTSAVQDGDEWVING
                     TKNWITNATIADFYVVFAVTDREAKRITAFVVDADSEGFSVPKLEHKIGIKGSPTGQP
                     VFEDVRVPAENVIGEVGRGMAVALSTLERTRLGAGAQAVGIAQGATDYANQYAKERIS
                     FGKPINQHQAIAFKLADMETKTAAARELLYTACAKADRNDKDLAKYSSMAKLFASDTA
                     MDVTVDAVQVLGGFGYVTEYPVERMMRDAKITQIYEGTNEIQRMVISRTMA"
     gene            complement(1894..2943)
                     /locus_tag="VFY44_06840"
     CDS             complement(1894..2943)
                     /locus_tag="VFY44_06840"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX5910192.1"
                     /translation="MFRRFFALAVLLCLLCAGGAVATLAWADKQAGDRIAQGVTVQGV
                     DVGGLTVAQAKSRLQRRIADPAQAPIVVTLDGKPHSLDARRAGVQVDVAGAAMQAAAE
                     GRGDGFLTRGWRELKGGAVDADIPLTVTVKRAEVDRFVAGLAKAADRPATDAGFSVSV
                     ESVGITEARPGRRLAGRESLAARIETALRAPGSHRDLTARTEPVQPKVTADQLRSRHP
                     TVVTVSRAARTIRVFDGGKVTKTYKAAVGEPNHPTPTGQFTVQSMQKNPTWNVPNSEW
                     AGDLAGKTIPGGDPDNPLVARWIGFNGSVGFHGTKEAGSLGQAASHGCVRMDPSDVKD
                     LFERVDVGTTVFVGG"
     gene            complement(3039..>3466)
                     /locus_tag="VFY44_06845"
     CDS             complement(3039..>3466)
                     /locus_tag="VFY44_06845"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX5910193.1"
                     /translation="PHTLVEFGDYSCPHTRAAQPELERLLNSRPDLRLVFRHLPLEDD
                     HPDAPLAAEAAEAAGVQGRFWDMHDRLLAETDTIPAAELVDHARALGLDLYRFSSDLR
                     YRVHAPAVAADVDSARRSGVPGTPAFYLDGEPVRALGEI"
BASE COUNT          439 a         1236 c         1225 g          566 t
ORIGIN      
        1 gtcagcttgg ccttcacctt gtcgacggcg ccggtgacct tctccttgat gtcgccggag
       61 gcctggtcga ccttgccctc gttcttgagg tccttgtccc cggtcacgga gccgacgcct
      121 tccttgaggc ggcccttcgc gtcgtcggcg ttcttcggtg aatcagccac gtttctccct
      181 gtcgtgtagt cggaatacgg ccacggttgc ggcgtcggcg atggtgccgg cgccaatcat
      241 ctcgtctacg tgcccgggag cgaaccgccg cacacgcagc ccctcctcct cggcctccag
      301 cgcctgctcg ccctgctcga ggccggtggc cagccaggcg tcgaagtact ggtccgagat
      361 cccgtaggcg aagtagagcc ggcccagccg ccgcatcgac gccgcccgca ggccggtctc
      421 ctcggccagc tcggtgcggg cgatgtgctc gggctccgcc tggtcctgac catcgagcgc
      481 gccctggggc agctcccaga accgctcgcc cacggggtgg cgccactgct ccaccagcgt
      541 gaccgacccg tcctcgtgca ccggcaggat caccgcggca gggcgcttct cgaccacgga
      601 gtacaggccg ggcgagccgt cgtcgcgctc gagcctgtcc tcgcgcagcc gcatccagcg
      661 gttctcgtag accacctcgg aggaggtcac ccgcggcggc tcgctcacgc catcgtgcgg
      721 gagatcacca tgcgctggat ctcgttggtg ccctcgtaga tctgggtgat cttggcgtcg
      781 cgcatcatcc gctcgacggg gtactcggtc acgtagccga agccgcccag cacctgcacg
      841 gcgtccacgg tcacgtccat cgcggtgtcc gaggcgaaca gcttggccat ggagctgtac
      901 ttggccaggt ccttgtcgtt gcggtcggcc ttggcgcatg cggtgtacag cagctcgcgg
      961 gccgcggccg tcttggtctc catgtcggcc agcttgaacg cgatcgcctg gtgctggttg
     1021 atcggcttgc cgaagctgat ccgctccttg gcgtactggt tggcgtagtc ggtggcgccc
     1081 tgggcgatgc ccaccgcctg cgcgccggcg ccgagccggg tgcgctcgag cgtggacagg
     1141 gcgacggcca tgccgcgccc cacctcgccg atcacgttct cggccggcac gcgcacgtcc
     1201 tcgaacaccg gctggccggt cggcgagccc ttgatgccga tcttgtgctc gagcttgggc
     1261 accgagaagc cctcggagtc ggcgtccacg acgaacgccg tgatgcgctt ggcctcgcgg
     1321 tcggtcacgg cgaacaccac gtagaagtcc gcgatcgtgg cgttggtgat ccagttcttg
     1381 gtgccgttga tgacccactc gtcgccgtcc tggacggcgc tggtgcgcat cgagccggga
     1441 tccgatcccg cctcgggctc ggacaggcag aacgcgggcg acttctcgcc ggaggcgcag
     1501 tcgggcaggt acttctgctt gagctcctcg ctgccgtgca gctggatcgg cagcgtgccc
     1561 agctcctgca ccatcaggat cagggccgag gacgcgcacg ccttggcgat ctcctcgacc
     1621 gccacgttca gcatcagcgt gccggtgccg gtcccgccgt actcctcggg gaagggcagg
     1681 cccaggatgt cgtgctcggc cagcgtccgg cgcacgtccc acgggtactc gccggtgctg
     1741 tcgatctcgg ccgcgcgcgg ggcgatctcg cccgccacca gctcgcggat ggtctgctgg
     1801 aagtccttga actcctgcgg gagcgtgtag gcgtcggctc cgggagtggc ggtggtgctc
     1861 atgccgggga gcctacgcga ggctcgccgg gcgctagccg ccgacgaaga ccgtggtgcc
     1921 cacgtcgacg cgctcgaaca ggtccttgac gtcggacggg tccatgcgca cgcacccgtg
     1981 cgaggccgcc tgccccagcg agccggcctc cttggtgccg tggaagccga ccgagccgtt
     2041 gaacccgatc cagcgcgcca cgagcgggtt gtccgggtcc ccgccgggga tggtcttgcc
     2101 ggccaggtcg cccgcccact cggagttggg cacgttccag gtcgggttct tctgcatgct
     2161 ctggacggtg aactgcccgg tgggcgtcgg gtggttgggc tcgccgaccg ccgccttgta
     2221 ggtcttggtc accttgccgc cgtcgaacac gcggatggtg cgcgcggcgc gcgagaccgt
     2281 gaccaccgtg gggtggcggc tgcgcagctg gtcggcggtg accttcggct ggacgggctc
     2341 ggtacgggcg gtcaggtcgc ggtggctgcc cggggcgcgc agggccgtct cgatcctggc
     2401 ggccagcgac tcgcgaccgg ccaggcggcg gccgggccgg gcctcggtga tgcccacgct
     2461 ctcgacgctg acgctgaagc ccgcgtcggt cgcggggcgg tcggcggcct tggccaaccc
     2521 ggcgacgaag cggtcgacct cggcccgctt gaccgtgacg gtcaggggga tgtccgcgtc
     2581 caccgcgccg cccttgagct cgcgccagcc gcgggtcagg aagccgtcgc cgcgtccttc
     2641 ggcggccgcc tgcatggcgg cgccggccac gtccacctgc acgccggcgc ggcgcgcgtc
     2701 cagcgagtgg ggcttgccgt ccagggtgac cacgatcggc gcctgcgcgg ggtcggcgat
     2761 ccgccgctgg agccgggact tggcctgggc cacggtcagg ccgccgacgt cgacgccctg
     2821 gacggtcacg ccctgggcga tccggtcacc cgcctgcttg tcggcccacg ccagggtggc
     2881 caccgctccg cccgcgcaga gcaggcacag cagcacggca agagcgaaga agcggcggaa
     2941 catccaacct cggatcggag acgacgacag gagcgacgac ggacgctagt acgcggtcgg
     3001 cgcccctgtc caaattcctg cgttttgcag cgtttcgttc agatctcgcc gagggcccgg
     3061 acgggctcgc cgtcgaggta gaacgccggc gtccccggga ccccgctgcg ccgcgcgctg
     3121 tccacatccg ccgccaccgc gggtgcgtgt acgcgatagc gcaggtcgct gctgaagcgg
     3181 tagaggtcca atccgagggc tctggcgtgg tccaccagct cagcggccgg gatcgtgtcg
     3241 gtctcggcca gcagccgatc gtgcatgtcc cagaaccgcc cctggacccc ggccgcttcg
     3301 gccgcctctg ctgccagcgg cgcatccggg tgatcgtcct ccagaggcag gtggcggaac
     3361 acgagccgca ggtccggacg cgagttcagc aaccgctcca gctcgggctg ggccgcgcgc
     3421 gtatgcgggc aggagtaatc gccgaactcg accagcgtgt gcggcg
//
LOCUS       DASUPW010000304         5257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_350719, whole genome shotgun sequence.
ACCESSION   DASUPW010000304 DASUPW010000000
VERSION     DASUPW010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350719"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="VFY44_06850"
     CDS             complement(<1..943)
                     /locus_tag="VFY44_06850"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX5910194.1"
                     /translation="MPGGVNSPVRALGSIGLDPISIASGAGAEITDVDGNVYLDWMCS
                     WGPLIAGHAHPAVIEAVTAAARRGTSFGATTEAELRLAEEVVDRVPSAEMVRMVNSGT
                     EAAMSAIRLARAATGREKLLKFAGAYHGHVDGLLAEAGSGLATASLPASPGVPAAQAA
                     ATVIVPWNDREAVAAACAEHEFAALLCEPYPANMGLIPPGEGFLSFLREQADANGALL
                     VFDEVISGFRVARGGAQERERVTPDLTILGKVIGGGVPAAAYAGRADLLRQVAPAGDV
                     YQAGTLSGNPLATAAGLATLEQLDRTAYERLRELTERL"
     gene            complement(975..2081)
                     /locus_tag="VFY44_06855"
     CDS             complement(975..2081)
                     /locus_tag="VFY44_06855"
                     /inference="COORDINATES: protein motif:HMM:NF037131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910195.1"
                     /translation="MASVDAAARAREEGATPKTRSRKDGSAIPLDDLDKKLLNLMQGS
                     FPLAPRPYEHVAGLAEVPESEVLTRVQRLVDGRIIRQVTPIFDTRVLGYSSMLVAAKV
                     DPENPWRAGKIINSHPGVSHNYLRNHDFNMWFTLATEPDSELGLQGTLDVLQELTGAE
                     SIRQLPTLKLFKIRMDLEMEKGTKDLAAMGVAQDPMEPDPIEVTDMDRAVIIALQGDM
                     PVVSEPYAPAAAEVGVTVPELLEHLESMKERKALRRVAAILFHRRAGFSANGMGVWNI
                     PEERIMEIAPLMASYRGISHCYQRPTYEDWPYSVFTMAHGRSKEECDAILDSIAQETG
                     IEDRRTLYSSTEFKKIRLLYYTDEHKRWEAEHAS"
     gene            complement(2069..3043)
                     /gene="hemB"
                     /locus_tag="VFY44_06860"
     CDS             complement(2069..3043)
                     /gene="hemB"
                     /locus_tag="VFY44_06860"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX5910196.1"
                     /translation="MAFPATRMRRLRRTGLLRGLVRETELTPAHLVQPYFVQLGTDER
                     TPIGSMPGVDRLSISHLVEEAGRAHAAGVPAVLLFGLPAEKDEQGSGAYDDEGVVQLA
                     VRALKDAHPDLVVVTDVCLCEYTSHGHCGVVRADGQVDNDLSLELLAKTAISHARSGA
                     DAVAPSDMMDGRVGALRAQLDAENLKDTPIISSSAKYASAFYGPFREAADSTPAFGDR
                     RSYQMDPANSDEAVRESLLDVEEGADMLMVKPALAYLDVLHRVKEASGLPVAAYNVSG
                     EYAMVEAAAAAGYIDRQAAIMETLTSIRRAGADVVFTYWATEVAEWLQ"
     gene            complement(3054..4400)
                     /locus_tag="VFY44_06865"
     CDS             complement(3054..4400)
                     /locus_tag="VFY44_06865"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5910197.1"
                     /translation="MAAVRHLLAKDLRILRRSPMLVALLVIYPVVIATLIGFALSRGP
                     DKPKVAFANLVPSSGNEIELGGKRIDVTRQAGPLFRAIDPVRVKTKAEAVRKVRDGEV
                     LAALVIPEDTTRKLQAGLEPAKVQVFYNADDPVKARYVRDTIKSQVQDANTALTKELS
                     KVALDFLNLIGTGGEYNFLGRQFDVLGLERSEQILEEAKADLPRGSPARAQIDQVIRF
                     ARLARENLDLSDDVLSSVSSPLKVEQTQLDGGATPLSSFAVAIAAAVSLMFITLLLAA
                     GALALEREENAYLRLVRGLVSRTALLFEKAGLAAVCSTVVVVLMLVGMGLFVDLDWGR
                     LPLAAAATAAGALAFATMGLAVGALTREVRAASLLCVMASLPLTFLALVPSGSVGPAL
                     YEVVRVVSAVFPFKPSLSALDVALNGGEDLGLHLLHLLALFAGFGALTRVALNRFA"
     gene            complement(4400..5101)
                     /locus_tag="VFY44_06870"
     CDS             complement(4400..5101)
                     /locus_tag="VFY44_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5910198.1"
                     /translation="MPLLEATGLVKRYGTREALRGVSVAATEGERVAVIGPNGAGKTT
                     LLSILAGIQRPDQGEISVPPGRAGWVPQQAALYGKLTVAENLRLFARLEKAEDPAGEV
                     ERMLDLTGLRERAGDQVSVLSGGNRQRVNIAIGLLGRQPVLLLDEPSAALDPRQRERV
                     WDFVLSLAEGGTTVVYSTHDIAEAERHANRVLVLADGDLIFTGAPGDLAREVESEEGA
                     DFEEAFVAFLKAKGH"
BASE COUNT          751 a         1891 c         1867 g          748 t
ORIGIN      
        1 caagacgctc ggtgagctca cgcaggcgct cgtacgcggt gcggtcgagt tgctccaggg
       61 tcgcgaggcc ggcggccgtc gccagcgggt tgcccgagag cgtgcccgcc tggtagacgt
      121 cgccggcggg cgccacctgg cgcaggaggt cggccctccc ggcgtacgcc gcggccggca
      181 caccgccgcc gatgaccttg cccaggatcg tcaggtcggg cgtgacgcgc tcgcgctcct
      241 gcgcgccccc gcgggccacg cggaagccgg agatgacctc gtcgaagacc agcagcgcgc
      301 cgttggcgtc ggcctgctcg cgcaggaagg acaggaaccc ctcgccgggc gggatcaggc
      361 ccatgttggc cgggtagggc tcgcacagca gcgcggcgaa ctcgtgctcg gcacacgctg
      421 ccgcgaccgc ctcgcggtcg ttccagggca cgatcaccgt ggccgcggcc tgggcggccg
      481 gcacgccggg gctggcgggc agcgacgcgg tggccagccc ggagccggcc tcggccagca
      541 gcccgtccac gtggccgtgg taggcaccgg cgaacttgag cagcttctcg cggccggtgg
      601 cggcccgcgc cagccggatc gcgctcatgg ccgcctcggt gccggagttg accatccgca
      661 ccatctcggc cgacggcacc cggtcgacga cctcctcggc caggcgcagc tcggcctcgg
      721 tggtggcccc gaagctggtg ccccgccggg ccgcggccgt gaccgcctcg atcaccgccg
      781 ggtgggcgtg gccggcgatc aacgggcccc acgagcacat ccagtccagg tagacgttgc
      841 cgtccacgtc ggtgatttcc gcgccggcgc ccgaggcgat cgagatcggg tcgaggccga
      901 tggagccgag cgcacggacc ggggagttca ccccgcccgg catcaggtcg atggcgcggg
      961 cgtacagctc ggcgctacga ggcgtgttca gcttcccacc gcttgtgctc gtcggtgtag
     1021 tacaggagcc ggatcttctt gaactcggtc gaggagtaca gggtccgccg gtcctcgatg
     1081 ccggtctcct gcgcgatcga gtccaggatg gcgtcgcact cctccttcga gcgcccgtgg
     1141 gccatggtga acaccgagta gggccagtcc tcgtacgtcg gccgctggta gcagtgcgag
     1201 atgccgcggt acgaggccat cagcggcgcg atctccatga tccgctcctc cgggatgttc
     1261 cacacgccca tgccgttggc cgagaagccc gcgcggcggt ggaacaggat cgcggccacg
     1321 cgccgcagcg ccttgcgctc cttcatcgac tccaggtgct ccagcagctc gggcaccgtg
     1381 acgcccacct cggccgccgc cggggcgtag ggctcggaca ccaccggcat gtcgccctgg
     1441 agggcgatga tcacggcgcg gtccatgtcg gtgacctcga tcgggtcggg ctccatcggg
     1501 tcctgggcca cgcccatggc ggccaggtcc ttggtgccct tctccatctc caggtccatc
     1561 cggatcttga acagcttgag cgtgggcagc tggcggatcg actcggcgcc ggtcagctcc
     1621 tggagcacgt cgagggtgcc ctgcaggccc agctcggagt cgggctcggt ggccagcgtg
     1681 aaccacatgt tgaagtcgtg gttgcgcagg tagttgtgcg acacgcccgg gtgcgagttg
     1741 atgatcttgc ccgcgcgcca ggggttttcc gggtcgacct tggccgcgac cagcatcgag
     1801 ctgtagccca ggacgcgggt gtcgaagatg ggcgtgacct gacggatgat gcgcccgtcc
     1861 acgagccgct gcacgcgggt cagcacctcg ctctcgggca cctcggccag gccggccacg
     1921 tgctcgtacg ggcgcggggc cagggggaag ctgccctgca tcaggttcag cagcttcttg
     1981 tccaggtcgt ccagcgggat ggccgagccg tccttgcggc tgcgcgtctt gggcgtggcg
     2041 ccctcttcgc gcgctctggc tgctgcgtct actgaagcca ttcggccacc tccgtcgccc
     2101 aataggtgaa gaccacgtcg gcgccggcgc ggcggatcga ggtcagggtc tccatgatcg
     2161 cggcctgccg gtcgatgtag ccggcggccg ctgccgcctc caccatcgcg tattcgccgc
     2221 tgacgttgta cgcggcaacc gggagcccgg atgcctcctt cacgcgatgg agcacgtcca
     2281 ggtaggccag cgcgggcttg accatcagca tgtccgcgcc ctcctcgacg tccagcagcg
     2341 actcgcgcac cgcctcgtcg gagttggccg ggtccatctg gtagctgcgc cggtcgccga
     2401 acgcgggcgt ggagtccgcg gcctcgcgga aggggccgta gaacgcggag gcgtacttgg
     2461 ccgaggagga gatgatcggc gtgtccttga ggttctcggc gtccagctgg gcgcgcagcg
     2521 cgcccacgcg gccgtccatc atgtcgctcg gcgcgacggc gtcggcgccg gagcgggcgt
     2581 gcgagatcgc ggtcttggcc agcagctcca gcgacaggtc gttgtccacc tggccgtcgg
     2641 cacggaccac gccgcagtgg ccgtgggagg tgtactcgca caggcacacg tcggtgacca
     2701 ccacgaggtc ggggtgggcg tccttgaggg cgcgcacggc cagctgcacc acgccctcgt
     2761 cgtcgtaggc gcccgagccc tgctcgtcct tctcggcggg caggccgaac agcagcaccg
     2821 cgggcacgcc ggccgcatgc gcgcggccgg cctcctcgac caggtgcgag atggacagcc
     2881 ggtcgacgcc gggcatggag ccgatcgggg tgcgctcgtc ggtgcccagc tgcacgaagt
     2941 agggctggac caggtgcgcc ggggtcagct cggtctcgcg tacgaggccg cgcagcaacc
     3001 ccgtacggcg caggcgccgc atgcgggtcg cgggaaaggc catcggaacg agtctaggca
     3061 aacctgttca gcgcgacccg cgtcagagcg ccgaagcccg cgaacagcgc cagcaggtgc
     3121 agcaggtgca ggcccaggtc ctcgcccccg ttcagcgcca cgtccagcgc gctcagcgac
     3181 ggcttgaacg ggaacacggc cgacaccacg cgcaccacct cgtacagcgc cggccccacc
     3241 gagcccgagg gcaccagggc caggaaggtc agcggcagcg aggccatgac gcacagcagc
     3301 gaggcggcgc gcacctcgcg cgtcagcgcc cccacggcca ggcccatggt ggcgaaggcc
     3361 agcgcgcccg ccgccgtggc cgcggcggcc aggggcaggc ggccccagtc caggtccacg
     3421 aacaggccca tgcccaccag catcaggacc accaccacgg tggagcagac ggccgccagg
     3481 ccggccttct cgaacagcag cgcggtacga gagaccagcc cccgcaccag cctcaggtag
     3541 gcgttctcct cccgttcgag cgccagcgcc ccggccgcca ggaggagcgt gatgaacatc
     3601 agcgacaccg ccgccgcgat ggccaccgcg aacgagctca gcggcgtcgc gccgccgtcc
     3661 agctgggtct gctcgacctt gagcggcgag ctgaccgagc tgagcacgtc gtccgacagg
     3721 tccaggttct cgcgcgccag ccgcgcgaac cggatgacct ggtcgatctg cgcgcgggcc
     3781 ggcgaccccc gcggcaggtc ggccttcgcc tcctccagga tctgctccga gcgctcgagg
     3841 ccgagcacgt cgaactggcg gccgaggaag ttgtactcac cacccgtgcc gatcaggttc
     3901 aggaagtcca gcgcgacctt cgacagctcc ttggtcagcg cggtgttggc gtcctgcacc
     3961 tgcgacttga tcgtgtcgcg cacgtagcgc gccttgaccg ggtcgtcggc gttgtagaac
     4021 acctgcacct tcgccggttc gaggccggcc tggagcttgc gggtcgtgtc ctcggggatg
     4081 accagcgcgg ccagcacctc gccgtcgcgg accttcctga ccgcctccgc cttcgtcttg
     4141 acgcgcacgg ggtcgatcgc ccggaacagc ggcccggcct gccgcgtcac gtcgatgcgc
     4201 ttcccgccca gctcgatctc gttgcccgac gacggcacca ggttggcgaa cgccaccttg
     4261 ggcttgtcgg gaccgcgtga gagcgcgaag ccgatcagcg tcgcgatgac cacggggtag
     4321 atcaccaaca gcgcgaccag catcggcgag cggcgcagga tccgcaggtc cttcgccagc
     4381 aggtggcgca ccgcggccat cagtggccct tcgccttgag gaacgcgacg aaggcctcct
     4441 cgaagtcggc cccctcctcg gactcgacct cccgggccag gtccccgggc gcgccggtga
     4501 agatcaggtc gccgtcggcc agcaccagca cgcggttggc gtggcgctcg gcctcggcga
     4561 tgtcgtgcgt ggagtacacg acggtggtcc cgccctcggc caggctgagc acgaagtccc
     4621 agacgcgctc gcgctggcgc gggtccaggg cggcgctggg ctcgtcgagc aacagcaccg
     4681 gctggcggcc caggagcccg atggcgatgt tcacgcgctg gcggttgccg ccggacagca
     4741 ccgacacctg gtccccggcg cgttcgcgca gcccggtcag gtccagcatc cgctcgacct
     4801 cgccggcggg gtcctcggcc ttctcgagcc tggcgaacag gcgcaggttc tcggccaccg
     4861 tgagcttgcc gtagagcgcc gcctgctgag gaacccaccc ggcgcggccc ggcgggacgc
     4921 tgatctcgcc ctggtccggg cgttggatgc cggccaggat cgagagcagc gtcgtcttgc
     4981 cggcaccgtt cgggccgatc accgcgacgc gctcgccctc ggtggccgca accgacacac
     5041 cgcgcagcgc ctcgcgcgtc ccatagcgct tgacgagccc tgtcgcctcg aggagcggca
     5101 agggccgaaa gctagacgtt gggagaatgc tgcggatgaa gctcctcttc gtcggagacg
     5161 tcgtcggaag cccgggccgc cacgcgctcg aggaggtcct gccgcggctc cgtgaggagc
     5221 accagccgga ctggatcgtc gtgaacgggg agaactc
//
LOCUS       DASUPW010000305         3062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_352040, whole genome shotgun sequence.
ACCESSION   DASUPW010000305 DASUPW010000000
VERSION     DASUPW010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352040"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="VFY44_06875"
     CDS             complement(<1..1039)
                     /locus_tag="VFY44_06875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX5910199.1"
                     /translation="MPGDRLTGLDASFLHLEDASAHMHVASVMVFEGDPPPYEDLLES
                     IEGRLHLVPRYRQRLAFVPLGQGRPRWVDDPHLNLRYHVRATALPAPGSEDQLRALAS
                     RVFAQQLDRDKPLWEIYLVEGLEGGRFALLAKTHHALVDGVSGVDIFSVLFDTSPEPA
                     VPPDPGRRWLPAPAPSSAQLLGEALLERATVPSEALRSVRALFRGPRQVANGVARAAY
                     GVGAMAWAGLNPAPSSIYNKRIGPHRRFTWVRTDLGDIKAIKNELGGTVNDVVLSTVA
                     GALGRHMRRRGQRTDGIELKAMVPVSVRAETERGDLGNKVAAMMAPLPVWCQEPEARL
                     EIVRDQMAGLKE"
     regulatory      1164..1339
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            1419..2042
                     /locus_tag="VFY44_06880"
     CDS             1419..2042
                     /locus_tag="VFY44_06880"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX5910200.1"
                     /translation="MLSASESQAADAATPATEAKPPVARAATVKAVSTRHVRAGRSAR
                     VRGKVAPAKAGRKVVLQVRTRKGWKTAKRGITRAGGKFRMKWKAPGAGRYRLRVKFRG
                     DTTATAAAAKSWRVNAYRGSHASWYGPGLYGNPLGCGGTLSAGTVGVAHKTLPCGTKV
                     TFRYRGRSVAARVVDRGPYVGGREWDLTAALKQKLGFPSTGTVLTTR"
     gene            complement(2032..>3062)
                     /locus_tag="VFY44_06885"
     CDS             complement(2032..>3062)
                     /locus_tag="VFY44_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5910201.1"
                     /translation="ETYKVGVAGPEQRAVAAAADAAGQDLQVKIESRRVRHRAQAEQL
                     VRDGDLDAALLEGRVVVSDEKLPDELAGALQVGQRQVATGATLRRQGLSESATRQVLE
                     PEPLKAETLSGDDDQEQKRFIALAAGILLYMQLLTYGLWVATGVVEEKSSRVVEILLS
                     TLSPRALMAGKIVGLGLLGMGQLLLVVAVGIALALATGSLDPNAGDISPALLVLLWFP
                     LGYALYASLYAATAALVSRQEDLQSATTPLTILILAAFFLSFNALDDPGGTLAQVASL
                     VPFSAPLVMPGRMALGDAAPVEVAVAVLLLVATTILLVRVGARVYAGAILRTGKPVKL
                     LEALRTSE"
BASE COUNT          436 a         1140 c         1089 g          397 t
ORIGIN      
        1 tctccttcag gccggccatc tggtcgcgca cgatctccag ccgcgcctcg ggctcctggc
       61 accacacggg cagtggggcc atcatggccg ccaccttgtt gcccaggtcg ccgcgctcgg
      121 tctcggcgcg cacgctgacc ggcaccatcg ccttgagctc gatcccgtcc gtgcgctgcc
      181 cacggcggcg catgtggcgc cccagggcgc cggccacggt gctgagcacg acgtcgttca
      241 cggtgccgcc cagctcgttc ttgatcgcct tgatgtcgcc caggtcggtg cgcacccagg
      301 tgaagcggcg gtgggggccg atccgcttgt tgtagatgct ggagggcgcg gggttcaggc
      361 cggcccaggc catggcgccc acgccgtagg cggcacgggc cacgccgttg gccacctgcc
      421 gcgggccgcg gaacagcgcg cggaccgacc gcagcgcctc gctgggcacg gtcgcgcgct
      481 ccagcagcgc ctcgcccagc agctgggccg acgacggggc gggagccggg agccagcgcc
      541 ggcccgggtc gggcggaacc gcgggctcgg gggaggtgtc gaacagcacg gagaagatgt
      601 cgacccccga gacgccgtcc accagcgcgt gatgggtctt ggccaggagc gcgaaccggc
      661 cgccctccag gccctcgacg aggtagatct cccacagcgg cttgtcgcgg tccagctgct
      721 gggcgaacac gcggctggcc agggcacgca gctggtcctc ggagccgggc gccggcagcg
      781 ccgtggcgcg cacgtggtag cgcaggttca ggtgggggtc gtccacccag cgcgggcggc
      841 cctggcccaa cggcacgaag gccaggcgct ggcggtagcg ggggaccagg tgcagccgcc
      901 cctcgatcga ctccaggagg tcctcgtagg gcggcggatc gccctcgaac accatcacgg
      961 aggccacgtg catgtgcgcc gacgcgtcct ccaggtgcag gaacgatgcg tcaagtccag
     1021 tcaggcgatc tccgggcatt gctccccttc ggtggtcgag ccctcaacct acccgcgtag
     1081 cggggcattt gcaccattcg ttgcggtgtg accaccctca cggaaggacg ccggggcgtc
     1141 tctaccgtgg cccgtcgttg ttcgattcgg ttgtgccgag cccgcctgcc tgaacaggca
     1201 gtcgaggcgg ggaacccatt gccgcaatac cgcggttggg gcgaatcgac ctctgcgcgc
     1261 agcgcgcgcg gaccgtcgta gcgacctccc cgggtcgcga gcccgtcagc taaccccgta
     1321 ggcgccgccg ggaaaggcat ccgttgttca agcgatccac cgtgcgtgca cggggtctgg
     1381 ctctggccgg cctcgccgta tgcgcaacca ccgccggcgt gctcagcgcg tcggagtccc
     1441 aggcggccga cgccgccacc cccgccaccg aggccaagcc gcccgtcgcc cgggcggcca
     1501 ccgtcaaggc cgtctcgacc cgccacgtgc gggccgggcg ctccgcgcgc gtgcgcggca
     1561 aggtcgcccc ggccaaggcc gggcgcaagg tcgtcctgca ggtcaggacc cgcaagggct
     1621 ggaagaccgc caagcgcggc atcacccgtg ccggaggcaa gttccgcatg aagtggaagg
     1681 ccccgggcgc cggccggtac cggctgcgcg tgaagttccg tggtgacacc accgccaccg
     1741 cggcggcggc caagtcgtgg cgcgtgaacg cgtaccgcgg ctcgcacgcc tcgtggtacg
     1801 ggcctggcct atacggcaac ccgctcggtt gcggcggcac gctgtccgcc ggcaccgtcg
     1861 gcgtggcgca caagacgctg ccgtgcggca ccaaggtcac cttccgctac cgcgggcgct
     1921 ccgtcgccgc gcgcgtggtg gaccgcggcc cctacgtggg cggccgcgag tgggacctga
     1981 ccgccgcgct gaagcagaag ctggggttcc cctcgacggg gaccgtgctg actactcgct
     2041 agtccgcagc gcttccagca gcttgaccgg cttgccggtc cgcaggatgg cgcccgcgta
     2101 gacgcgggcg cccacccgca ccaggaggat ggtggtggcc acgaggagca gcacggccac
     2161 cgccacctcc accggtgcgg cgtcgcccag ggccatccgc ccgggcatga ccaggggcgc
     2221 cgagaacggg accagcgacg ccacctgggc cagcgtgccg cccgggtcgt ccagcgcgtt
     2281 gaaggacagg aagaacgcgg ccaggatcag gatcgtcagc ggcgtggtgg ccgactgcag
     2341 gtcctcctgg cgtgagacca gcgccgccgt ggccgcgtac agcgacgcgt acagggcata
     2401 gcccagcggg aaccacagca gcaccagcag cgccgggctg atgtcacccg cgttcggatc
     2461 cagcgagccc gtggccagcg cgagcgcgat ccccacggcc accaccagca gcagctgtcc
     2521 catccccagc aggccgagcc ccacgatctt gccggccatc agggcccggg gggagagggt
     2581 ggacagcagg atctcgacca cccgcgagga cttctcctcg accacacccg tggccaccca
     2641 gagcccgtac gtcagcagct gcatgtacag caggatcccc gccgccaggg cgatgaaccg
     2701 cttctgctcc tggtcgtcgt cgcccgagag cgtctcggcc ttcagcggct cgggctccag
     2761 cacctggcgg gtggccgact ccgacaggcc ctggcggcgc agcgtggcgc cggtggccac
     2821 ctggcgctgg cccacctgga gcgcgccggc caactcgtcc gggagcttct cgtcggacac
     2881 caccacgcgg ccctccagca gcgcggcgtc caggtcgccg tcgcggacca gttgctcggc
     2941 ctgcgcccgg tggcgtacgc gccgggactc gatcttcacc tgtagatcct ggccggccgc
     3001 atcggcggcg gccgcgacgg cacgctgctc gggaccagcc acgccgacct tgtaggtctc
     3061 cg
//
LOCUS       DASUPW010000306         2672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1044035, whole genome shotgun sequence.
ACCESSION   DASUPW010000306 DASUPW010000000
VERSION     DASUPW010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044035"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VFY44_06890"
     CDS             <1..854
                     /locus_tag="VFY44_06890"
                     /inference="COORDINATES: protein motif:HMM:NF015406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HEX5910202.1"
                     /translation="LADLGVEDEPELVFEPGETAARKAMSSFLREPVGRYKELHDNAA
                     RGTSRLSPYLRWGCLSPLELEAKASEKRSNGADAFVRQLAWRDFYAAALMHFPHVVTQ
                     EFQERYRELEWKRPSKKLDAWKEGRTGFPFVDAGMRQLLAEGWMHNRLRLVVGSFLTK
                     DLHLDWRLGEAHFMEHLVDGDMAANNGNWQWIASVGMDPKPYFQRLFNPMLQQRKFDP
                     DGEYVRRWVPELADVPDSHLVEPWTMSQGDQQMAGCVIGEHYPEPIVDHAEERRVAQE
                     RYRSVPS"
     gene            complement(833..1234)
                     /locus_tag="VFY44_06895"
     CDS             complement(833..1234)
                     /locus_tag="VFY44_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910203.1"
                     /translation="MGEMSVKALAGLRIVVGLLSWFAPNLAGTLFGLDVKKNKQAPYL
                     ARLFGVRDIALGVGTLMAKGSARRTWLQMGLLADTADAAAAGIGHRDGYLDGQTAIML
                     AAPAVTAVALGSAALGIGDDSDTERQDGTER"
     gene            1276..2562
                     /locus_tag="VFY44_06900"
     CDS             1276..2562
                     /locus_tag="VFY44_06900"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX5910204.1"
                     /translation="MTPETKSTLFEGVGARLEAAAEVAEVSEDTLERLRLPQSVLKVS
                     VPVRMDDGSLKTFPGYRVRYDATRGPTKGGIRFHPRVNVDEVQSLAFWMTFKCACMDL
                     PFGGAKGGITVDAKELSLAEKERLSRNYIARIADMIGPDIDIPAPDMYTDQMVMGWMV
                     DEYSTIKRAQTPAAMTGKPLALGGSLGRDTATADGAFHVLKTLAPKLAEVADDDEPEL
                     QTVAIQGFGNAGARMAELCADAGYTVVAASDSTRAVYSEDGLDVAALREAKEADGELP
                     ADAGDELDPEDLLALEVDVLIPAALEDVITEDNAGDVRARVILEVANGPVAVEADQTL
                     YDNGVTVIPDILANAGGVTVSYFEWAQNRSGVRWSAEDVAERLEQKMVEQSEEVWDCA
                     RDREVTLRTAAYVVGLERISESVNATGSVEQFRNVR"
BASE COUNT          416 a          927 c          968 g          361 t
ORIGIN      
        1 ccctggccga cctgggcgtg gaggacgagc cggagctggt cttcgagccc ggcgagacgg
       61 ccgcgcgcaa ggccatgtcc tccttcctgc gcgagccggt gggcaggtac aaggagctgc
      121 acgacaacgc cgcgcgcggc acctcgcggc tgtcgcccta cctgcgctgg ggctgcctgt
      181 cgccgctcga gctggaggcg aaggcctccg agaagcggag caacggcgcc gacgccttcg
      241 tccgccagct ggcctggcgc gacttctacg cggccgcgct gatgcacttc ccgcacgtgg
      301 tcacgcagga gttccaggag cgctaccgcg agctggagtg gaagcggccg tcgaagaagc
      361 tggacgcgtg gaaggagggc cgcaccggct tcccgttcgt ggacgcgggc atgcgccagc
      421 tgctggccga gggctggatg cacaaccggc tgcggctggt cgtgggctcg ttcctgacca
      481 aggacctgca cctggactgg cgcctgggcg aggcccactt catggagcac ctggtggacg
      541 gcgacatggc ggccaacaac ggcaactggc agtggatcgc ctcggtgggc atggacccca
      601 agccctattt ccagcggctg ttcaacccga tgctgcagca gcgcaagttc gaccccgacg
      661 gcgagtacgt gcgccgctgg gtgcccgagc tggccgacgt gcccgactca cacctggtcg
      721 agccctggac catgagccag ggcgaccagc agatggccgg gtgcgtgatc ggcgagcact
      781 accccgagcc gatcgtggac cacgccgagg agcggcgcgt ggcccaggag cgctaccgct
      841 cggtgccgtc ctgacgctcg gtgtcggagt cgtcgccgat gcccagcgcc gcggagccca
      901 gggccaccgc cgtcaccgcg ggcgcggcga gcatgatcgc ggtctgcccg tccaggtagc
      961 cgtccctgtg gccgatcccg gcggccgccg cgtcggcggt gtcggccagc agccccatct
     1021 ggagccaggt gcgccgcgcc gagcccttgg ccatgagcgt gcccacgccg agcgcgatgt
     1081 cacgcacgcc gaacagccgc gccaggtacg gcgcctgctt gttcttcttg acgtcgagcc
     1141 cgaagagcgt cccggccagg ttcggcgcga accacgacag aagtccgacc acgatccgca
     1201 gccccgcaag tgccttgacg ctcatttctc ccatgcgggt gatcctatac cggcggatcc
     1261 gggcacaggg gtcagatgac tccagagacc aagagcacgc tgttcgaggg cgtcggcgcc
     1321 cggctggagg ccgcggccga ggtcgccgag gtgtccgagg acacgctcga gcgcctgcgc
     1381 ctgccccagt cggtgctgaa ggtgtcggtg ccggtgcgca tggacgacgg ctcgctgaag
     1441 acgttccccg gctaccgggt ccgctacgac gccacccgcg gcccgaccaa gggcggcatc
     1501 cgcttccacc cgcgcgtgaa cgtggacgag gtgcagtcgc tggccttctg gatgaccttc
     1561 aagtgcgcct gcatggacct gccgttcggg ggcgcgaagg gcggcatcac ggtggacgcc
     1621 aaggagctct cgctggccga gaaggagcgc ctgtcgcgca actacatcgc ccggatcgcg
     1681 gacatgatcg gccccgacat cgacatcccc gcgcccgaca tgtacaccga ccagatggtc
     1741 atgggctgga tggtcgacga gtactcgacc atcaagcgcg cgcagacccc ggcggcgatg
     1801 accggcaagc cgctggccct cggcgggagc ctgggccgcg acaccgccac cgccgacggc
     1861 gccttccacg tgctcaagac gctggccccc aagctggccg aggtggccga cgacgacgag
     1921 cccgagctgc agaccgtggc gatccagggc ttcggcaacg ccggcgcgcg catggccgag
     1981 ctgtgcgccg acgccggcta caccgtggtc gcggcctccg actccacgcg cgcggtgtac
     2041 tccgaggacg gcctggacgt ggccgccctg cgcgaggcga aggaggccga cggcgagctg
     2101 cccgccgacg ccggcgacga gctcgacccc gaggacctgc tggcgctgga ggtcgacgtg
     2161 ctgatccccg cggcgctgga ggacgtgatc accgaggaca acgctggcga cgtccgcgcg
     2221 cgcgtgatcc tggaggtggc caacgggccg gtggccgtgg aggccgacca gacgctgtac
     2281 gacaacggcg tcacggtgat ccccgacatc ctggccaacg ccggcggggt gaccgtgtcc
     2341 tacttcgagt gggctcagaa ccgcagcggc gtgcgctgga gcgccgagga cgtggccgag
     2401 cggctggagc agaagatggt cgagcagtcc gaggaggtct gggactgtgc ccgcgaccgc
     2461 gaggtcacgc tgcgcaccgc cgcctacgtg gtcgggctgg agcgcatctc cgagtcggtg
     2521 aacgcgaccg ggtcggtcga gcagttccgc aacgtccgct gacgcgcttg agatgaccgc
     2581 gctccgggca aggcccggcc atggagacga ggagcctgcg agagaacgcc cgggtgtact
     2641 tcttcggagc gctcgggggg atgctgttcg gc
//
LOCUS       DASUPW010000307         4846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1737312, whole genome shotgun sequence.
ACCESSION   DASUPW010000307 DASUPW010000000
VERSION     DASUPW010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1737312"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1873)
                     /locus_tag="VFY44_06905"
     CDS             complement(<1..1873)
                     /locus_tag="VFY44_06905"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX5910205.1"
                     /translation="MILGVDVGGTFTDAVLLTGDGVVTGKAPSTPADQSEGVMAAVAQ
                     ALDAAGVEAGDVQRLVHGMTVGTNALLEGTTARTALVATEGFTDIEELGRQARPELYR
                     LCAGRPEPVVGRDLRVAAPERCGPDGVIAALDEDLLRERLAALRDQDVEAVAVCLLWG
                     LRHPDHERRVAELAAEALPDAHVSTSHGTAAVFREYERLATTTVDAAVSPLLRRYLRR
                     LTERTGEAGLPDPEIMLSSGGVADASTAAAHGSWTVLSGPAGGAVAAARAAGAAGAPG
                     AVGLDMGGTSCDVSLVLDGRVAVTGGREVGGRSLALPMIDVHTVGAGGGSIAWRDQGG
                     ALRVGPRSAGADPGPACYGRGGTEPTVTDANLLLGYLDAGAPLAGDVRLDRDAAEQAL
                     GGLAGELDLSVEELAAGIVRVAATEMAQAARVVTVERGVDPRELALVSFGGAGGLHAA
                     GIAEELGMSTVIAPVVGGVLSALGLAVSERRRDLVESVLLSGEELTAEAVAGVVARLA
                     ERGRDELGAPEAEVRPTYELRYGGQAFELPVEAGPEPDPDELRGGFDAAHEERYGYRD
                     PDAQLELVTVRVAVALPGSELEPATAGEAERRGTRSARFGGRVLDAAVFGPGTAEVEG
                     "
     gene            complement(1920..2342)
                     /locus_tag="VFY44_06910"
     CDS             complement(1920..2342)
                     /locus_tag="VFY44_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910206.1"
                     /translation="MYQFSRAIYRELVDEVGPGNEVQRCMARQRLLAECEAAMERLAT
                     DRHYFAKPVRTLFNDIRCLFPMGSQMHVYRVIEHQVTMAAEYVDSQAREGVTFDGSPL
                     CCHATTRKGTSCQRVPLPGSKYCPSHKHLDELGVVAAA"
     gene            complement(2489..2986)
                     /locus_tag="VFY44_06915"
     CDS             complement(2489..2986)
                     /locus_tag="VFY44_06915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX5910207.1"
                     /translation="MPVDNVPELFHEAMAHMASGVAVITARRDDGEPCGLAATAVASY
                     SADPPSLLVSVSHDSRCHDHLEACAQFGVHILGADQEALARTFASKGDDKFADLAWSW
                     DQDVPELEGTLAYLRCRRAENFVRYDHTILIGDIAGGRVGSADPLLYARRRMDWALQA
                     GDGPL"
     gene            complement(3009..4484)
                     /locus_tag="VFY44_06920"
     CDS             complement(3009..4484)
                     /locus_tag="VFY44_06920"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5910208.1"
                     /translation="MAPHDAPISESADMIVVGAGAAGLFCALVAADAGASVALVSRTP
                     LAGSASYWAQGGIAAALAVDDSPARHAEDTIAAGRGVARPSAVDVLCRDSPDRVRDLE
                     RMGVRFDADRHGALALGLEGGHSARRIVHAGGSATGRRITRQLSALAARHERVRVMED
                     TTATGLLVQDGRCVGVAARSQAGSLAVAARGTVLATGGSAALWSRSTNPAGSVGVGLA
                     LAHAAGARLADLEFMQFHPTALVSDSARDGFLITEAVRGEGALLLDAEGNRFVDELAP
                     RDQVALAIEQRLREQGTRSVGLDLRPVDLARFPNIADALAAEGIDPARDLVPVAPASH
                     YTMGGVATDLDGRASLEGLLAVGECACTGLHGANRLASNSLAECFVLGRRAAHAVLDV
                     PGEPPAPAAAEAASLPDPPSAATRAALWRHAGLRRSPEGLRELAGDPFPLARLIAAAA
                     LAREESRGAHQRVDHPATDPALDDHHVLLGPGQQVELEKWR"
     gene            4473..>4846
                     /locus_tag="VFY44_06925"
     CDS             4473..>4846
                     /locus_tag="VFY44_06925"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910209.1"
                     /translation="MRGHEKPGYRRAIAAPLYLRHPASLDHDTGQHPERPQRIRALET
                     LMDSRGWLGWDVQEAPRATEEQLHAVHPPSYVESVRAHCEAARPLDGDTPVVPASWEA
                     GLRAAGGACALVDALLDGTAPTG"
BASE COUNT          606 a         1912 c         1770 g          558 t
ORIGIN      
        1 ggccctctac ctcggccgtg ccgggcccga agacggccgc gtccagcacc cgtccgccga
       61 agcgcgccga gcgcgtgccc cgtcgctcgg cctcgcccgc ggtggccggc tccagctccg
      121 agccgggcag cgcgaccgcc acgcgcaccg tcaccagctc gagctgcgcg tcggggtcgc
      181 ggtagccgta gcgctcctcg tgggcggcgt cgaagccacc gcgcagttcg tcggggtcgg
      241 gctcgggccc cgcctcgacg ggaagctcga aggcctggcc gccgtagcgc agctcgtagg
      301 tgggccgcac ctccgcctcg ggcgcgccca gctcgtcgcg cccgcgctcg gccagccggg
      361 ccaccacccc cgccacggcc tccgcggtca gctcctcacc ggagagcagg acgctctcga
      421 ccaggtcgcg ccggcgctcc gacaccgcca ggccgagggc cgacagcacc ccgcccacca
      481 ccggcgcgat gaccgtggac atgcccagct cctccgcgat cccggcggcg tgcaggccac
      541 ccgcgccgcc gaagctgacc agcgccagct cgcgcgggtc cacgccgcgc tcgacggtga
      601 ccacgcgggc cgcctgggcc atctcggtgg cggccacgcg aacgatgccc gccgcgagct
      661 cctcgaccga caggtccagc tcgcccgcca ggccgcccag ggcctgctcg gccgcgtcgc
      721 ggtccaggcg cacgtcgccg gccagcggcg cgcccgcgtc caggtagccc agcagcaggt
      781 tggcgtcggt caccgtcggc tcggtgccgc cgcgcccgta acaggcggga cccgggtcgg
      841 cgccggccga gcgcggcccg acccggagcg cgccgccctg gtcgcgccag gcgatgctgc
      901 cgccgccggc gcccaccgtg tgcacgtcga tcatcggcag cgccagcgag cgcccgccga
      961 cctcgcgccc accggtgacg gcgacccggc cgtccagcac cagcgacacg tcgcaggagg
     1021 tgccgcccat gtccagcccc accgctcccg gcgccccggc ggcgcccgcc gcccgggccg
     1081 cggccacggc gcccccggcc ggcccggaga ggacggtcca cgagccgtgc gccgccgccg
     1141 tggacgcgtc ggccacgccg ccgctggaga gcatgatctc ggggtcgggc agcccggcct
     1201 cgccggtgcg ctcggtcagc cgccgcaggt agcgccgcag cagcggcgag accgcggcgt
     1261 cgaccgtcgt ggtggcgagc cgctcgtact cgcggaacac ggccgcggtg ccgtgtgagg
     1321 tggacacgtg cgcgtccggc agagcttcgg cggccagctc ggccacccgc cgctcgtggt
     1381 cggggtggcg caagccccac agcaggcaca cggccaccgc ctcgacgtcc tggtcgcgca
     1441 gcgccgccag ccgctcgcgc aacaggtcct cgtcaagcgc ggcgatcacg ccgtcgggcc
     1501 cgcagcgctc gggggcggcg acgcgcaggt cgcgcccgac caccggctcg gggcggccgg
     1561 cgcacaggcg gtagagctcc ggccgggcct ggcggccgag ctcctcgatg tcggtgaagc
     1621 cctcggtggc caccagcgcc gtgcgggcgg tggtgccctc gagcagggcg ttggtgccca
     1681 cggtcatgcc gtggaccagg cgctggacat cgcccgcctc cacgccggcg gcgtccaggg
     1741 cctgggccac ggcggccatg accccctcgg actggtccgc gggggtggac ggcgccttgc
     1801 cggtcacgac cccgtcgccg gtcagcagca ccgcgtcggt gaaggtcccg ccgacgtcga
     1861 cgcccaggat cattgcgccg aagctaacgc ctcggcatgg ctatgtcgta cgagcgcttc
     1921 taggcggcgg ctacgacgcc gagctcgtcc aggtgcttgt gcgacgggca gtacttgctg
     1981 cccggcagcg gcacccgctg gcacgacgtc cccttgcgcg tcgtggcgtg gcagcacagg
     2041 gggctgccgt cgaacgtgac gccctccctg gcctggctgt ccacgtactc ggcggccatc
     2101 gtgacctggt gctcgatcac gcggtacacg tgcatctggc tgcccatcgg aaacaggcag
     2161 cggatgtcgt tgaacagcgt gcgcaccggc ttggcgaaat agtgcctgtc ggtggccagc
     2221 cgctccatgg cggcctcgca ctcggccagc agacgctggc gcgccatgca gcgctgcact
     2281 tcgttccccg gcccgacctc gtcgacaagc tcgcgataaa tcgcgcgact gaactggtac
     2341 atgcttcctc ctccggcgac ctcccgcggc cgccccacat cgtcggctga gccttatacc
     2401 caggcccgtc tctaatcaga acgcacaaga cgttaaaagt gcattaaccc gtgcgaatcc
     2461 gcactagagg gcgcgtctgc gttccccctc agaggggacc gtcaccggcc tggagcgccc
     2521 agtccatgcg cctccgggcg tacagcagcg ggtcggccga gcccacgcgc ccgccggcga
     2581 tgtcgccgat caggatcgtg tggtcgtagc ggacgaagtt ctccgcgcgc cggcagcgca
     2641 ggtaggccag cgtgccctcc agctcgggca cgtcctgatc ccagctccag gccaggtcgg
     2701 cgaacttgtc gtcgcccttg gacgcgaacg tccgcgccag ggcctcctgg tcggcgccca
     2761 ggatgtgcac gccgaactgc gcgcaggcct cgaggtggtc gtggcagcgt gagtcgtggc
     2821 tgaccgacac cagcagcgac ggcgggtccg cgctgtagga ggcgaccgcg gtggcggcca
     2881 ggccgcaggg ctccccgtcg tcccggcggg ccgtgatcac ggccaccccc gacgccatgt
     2941 gcgccatggc ctcgtggaac agctcgggta cgttgtcgac cggcacgggg ggcaatccta
     3001 cgcggccgtc atcgccactt ctcgagctcc acctgctgcc cggggcccag cagcacgtgg
     3061 tggtcgtcca gcgcggggtc ggtggcgggg tggtcgacgc gctggtgcgc cccgcggctc
     3121 tcctcgcggg ccagcgcggc cgccgcgatc agccgggcga gcgggaacgg gtcgccggcc
     3181 agctcgcgca ggccctccgg gctgcggcgc aggccggcgt ggcgccagag cgccgccctg
     3241 gtggcggcgg acggcgggtc gggcagcgag gccgcctcgg cagccgccgg cgcgggcggc
     3301 tcaccgggaa cgtccaggac cgcgtgcgcg gcgcggcggc ccagcacgaa gcactcggcc
     3361 agcgagttcg aggccagccg gttggccccg tgcaggccgg tgcacgcgca ttcgcccacg
     3421 gccagcaggc cctccagcga cgcgcgcccg tccaggtcgg tggccacccc gcccatcgtg
     3481 tagtgcgagg ccggggccac cggcaccagg tcgcgggcgg ggtcgatgcc ctcggcggcc
     3541 agagcgtcgg cgatgttggg gaagcgggcc aggtccaccg ggcgcaggtc cagccccacc
     3601 gaccgcgtgc cctgctcgcg cagccgctgc tcgatggcca gcgccacctg gtcgcgcggc
     3661 gccagctcgt ccacgaaccg gttgccctcg gcgtccagca gcaacgctcc ctcgccgcgc
     3721 accgcctcgg tgatcaggaa accgtcgcgg gcgctgtcgc tgaccagcgc ggtggggtgg
     3781 aactgcatga actccaggtc ggccaggcgg gcgcccgccg cgtgggccag ggccaggccg
     3841 accccgaccg acccggccgg gttcgtcgag cgcgaccaca gcgccgccga cccgcccgtg
     3901 gccagcacgg tgccgcgcgc ggcaacggcc agcgacccgg cctgcgagcg cgcggccacg
     3961 cccacacagc gcccgtcctg caccagcaac ccggtcgcgg tggtgtcctc catcacccgg
     4021 acccgctcgt ggcgcgcggc cagcgccgac agctggcgcg tgatccggcg gccggtggcc
     4081 gacccgcccg cgtgcacgat ccggcgcgcc gagtgcccgc cctccaggcc cagggccagc
     4141 gccccgtggc ggtcggcgtc gaagcgcacg cccatgcgct cgaggtcgcg gacgcggtcg
     4201 ggcgagtccc ggcagagcac gtccaccgcg ctcggccggg ccacgccgcg gcccgccgcg
     4261 atcgtgtcct cggcgtggcg ggcggggctg tcgtcgaccg ccagcgcggc ggcgatgccg
     4321 ccctgggccc agtagctggc agagccggcg agcggcgtgc gcgagaccag cgccaccgat
     4381 gcgccggcgt cggcggccac gagcgcgcag aagagccccg cggcgccggc gcccacgacg
     4441 atcatgtctg ccgactcgga gatgggggcg tcgtgcgggg ccacgagaag cccggatacc
     4501 gtagagccat cgccgctccg ctctacctgc gccaccccgc gagcctggac cacgacacgg
     4561 ggcaacaccc cgagcgtccc cagcgcatcc gcgcgctgga gacgttgatg gacagccgcg
     4621 gctggctggg ctgggacgtc caggaggccc cgcgcgcgac cgaggagcag ctgcacgcgg
     4681 ttcacccgcc ctcctacgtg gagtcggtcc gcgcgcactg cgaggccgcg cgcccgctgg
     4741 acggcgacac cccggtcgtg cccgcgtcgt gggaggcggg cctgcgtgcc gcgggcggcg
     4801 cgtgcgcgct ggtcgacgcg ctgctggacg gcacggcgcc cacggg
//
LOCUS       DASUPW010000308         7233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1320859, whole genome shotgun sequence.
ACCESSION   DASUPW010000308 DASUPW010000000
VERSION     DASUPW010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7233
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320859"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VFY44_06930"
     CDS             <1..414
                     /locus_tag="VFY44_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910210.1"
                     /translation="KAPLAVGAGIVATAALVIAVALGSPESDPTAATPPAQAAPAPAE
                     PKAKAAAPAAEPASPGRPPAAVSGYSDLGPGVLGTLEGSGAATGSGAGASARRSADGG
                     SLPFTGLDLALLGLAGAALLGTGAALRRLASAPRL"
     gene            425..2386
                     /locus_tag="VFY44_06935"
     CDS             425..2386
                     /locus_tag="VFY44_06935"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX5910211.1"
                     /translation="MPNRLAGETSPYLLQHKDNPVDWYPWGPEALDAARERDVPILLS
                     IGYSACHWCHVMERESFEDPATAAFMNDRFVCIKLDREERPDLDAIYMEACQAMTGHG
                     GWPLNVFLTPEQVPYYAGTYFPPEDRQGMPSWTTVLDAVREAWDERADEIRATSGKVA
                     ERLRGGAGLEPAAEALDATVLEAAVEGLRSVYDAENGGFGGAPKFPPASTVEFLLARG
                     EQDMSLHTLRAMAGGGMYDQVGGGFARYSVDARWVVPHFEKMLYDNALLARAYLHGWQ
                     ASGDELLRRVAEETLDWMLTELRGPEGGFMSALDADSEGEEGLFYVWTPDQLREVLGP
                     EDAEAAAAYFGVQPGGNFEGRTILTRGDAEPENLADIRRRLSEARARRVWPGLDDKRL
                     TSWNALAIAALAEAGAVLEQPRFLDAARDAADFVLRDLRDADGRLLRTYKDGHAKLNA
                     YLEDHAFLLDALLVLYQATFEPRWFSEARALADTMIERFADDERGGFFETSDDHEKLV
                     ARRKDLEDHPIPSGNSAAAMGLLRLAALTGEYAYEQRAAGVLALLHPIAAKHPQAFSN
                     LLLAADFHLAAVREVALAGDDTSELERVVRARLRPHLVLAGGDPAGVPLLQGREPVDG
                     EPAAYVCERFACLRPVTRPAELEALLASP"
     gene            complement(2343..4841)
                     /locus_tag="VFY44_06940"
     CDS             complement(2343..4841)
                     /locus_tag="VFY44_06940"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5910212.1"
                     /translation="MSSPAPARKSPRSSIFAVPGGSRAKWVVLAVWLVGMIVAFGANL
                     PGKFTDAEENESTSFLPGDAESTKALKATEELQSGELAPAVIVFRRESGLTASDRETI
                     TKDVAALAKKRYKGVVADGSTAASGGKQGGDSEGGPRPTGKAAAPGCAGPTTPVRGQP
                     SGYQPFVGPICSADGKAAIVTAYLKGDGESDSLLDPIQDWRDKVSDPGGGLEVKITGG
                     AGYAADAIEVFESINGTLLLAAVSLVIILLILIYRSPVFLFVPLLAVIFAEMLSRSVG
                     YGLSEVGVTINGQSSSIMSVLVLGAGTDYALLIVSRYREELHHTSDRHVAIAEAMRSA
                     GPAVFASAATVVAALLCLTLAKVNGTAGLGPIGAMGVACAALSMLTLLPALLTIFGRR
                     ALWPFVPHTPETAPTEAVLRSGIARKVAEGSKPVALGLVVLAGLVVAVFLPVVLLFRL
                     VMFVLGKATGAHFRPLHDHVFLPYELRRYRHEKPRDATHGFWARVGTRVAASPRRVAT
                     GSVVLLLIMTAGLAFFSTDLTTNDSYTTDVESVEGQDLLARSFPSGASVPTDIVVPRG
                     GDVAAVTAAAEGVDGVEAVTPPVAEGAQGTLIQATLEPNPYSTEAFDLVEPLRDAVSG
                     AQDGTIVGGASAVEFDVRDASAWDSTVIPPIVLVVVFLILVLLLRAVIAPLILIGTVI
                     LSFVAALGVGYFAFDVLFGFPGSDPSLPLFAFVFLVALGVDYNIFLVARAREETLKYG
                     TREGMTRALAVTGGVITSAGIVLAGTFMVLGSLPLVFLAEIGFVVAFGVLLDTFLVRS
                     VLVPALILQMGDKVWWPSALSKGSPAKPPAQPVA"
     gene            4948..5703
                     /locus_tag="VFY44_06945"
     CDS             4948..5703
                     /locus_tag="VFY44_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX5910213.1"
                     /translation="MRGIRHGTRALTALALVGALAVAVPATAATIKGSKRADRITGTK
                     RADKIKAGRGNDRVRSGRGADRVYGGRGNDRIDGGSSADRLYGEPGNDRVRGRGGDDR
                     ARGGAGRDRLTTDSGDDLLYGDAGDDRLSAGFGNDRVFGGSGNDLLFAGDPGRGASPG
                     DDLAGQAGNDRLRGDTGNDGLDGGPGNDRIDGGRGEDDLIGGEGNDRIDARDGEQDTV
                     DCGDGQDTLLADSLEDGVTECETVLVPPDPPEA"
     gene            complement(5713..7164)
                     /locus_tag="VFY44_06950"
     CDS             complement(5713..7164)
                     /locus_tag="VFY44_06950"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX5910214.1"
                     /translation="MREEDLSRRDFLERTAYAAGLAGMAGAVPASTLLYHAADAEARR
                     ARLPSPRNVEIDHFVVLMMENRSFDHYFGWAPGADGKQKQSFRDPAGNMVPTRPASNL
                     GANSQWKGCGYNDPGHGWKSGRAQLRNGFMGQGSGNDIFALSYYDRGQLGYIHQAAST
                     YTLYDRYFCSLLGPTFPNRYYKWAATSAGKTSNIEQVDVAKGGNQFETIFDRAIGRGL
                     SARYYHADIPFAAVYGARGLGWANPVSRYYADCAAGTLPNITFIDPPFGDGGGGDGLS
                     ADEHPLGDVRLGQAFMADVVNAFVASPNYRRGALFIVYDEWGGFFDHVKPPRVPDKRS
                     TANLDTDFGQMGFRIPAVTVSPYTRRRTTGDQLRVDHGVYGHESILKLISHRFGLGHL
                     TRRHRYARNIGHSFDWANPDFNRPSLPDPPAVATQPCSMGGGDITDQRDAHADDLAGL
                     EDVAHRYRYRVYDGKPHEIFTKPDSVKKAYKPR"
BASE COUNT         1027 a         2610 c         2664 g          932 t
ORIGIN      
        1 aaggcaccgc tggccgtcgg cgccggcatc gtcgcgacgg cggcgctggt gatcgcagtc
       61 gctctcggct cgcccgagtc ggacccgacc gcggccacgc cgccggccca ggcggcaccc
      121 gccccggccg agcccaaggc caaggccgcg gcgcccgcgg cggagcccgc ctcccccggc
      181 cgccccccgg cggccgtgtc gggctactcc gacctgggcc cgggcgtgct gggaaccctt
      241 gaggggtcgg gggccgcaac gggctccggc gccggcgcct ccgcccgacg gtccgccgac
      301 ggcgggtcgc tgcccttcac cggcctggac ctggcgctgc tggggctggc cggggcggcg
      361 ctgctgggaa ccggggccgc gctgcgccgc ctggcctccg cgccgcggct ctagcctcgg
      421 aggcatgccc aaccgccttg cgggcgagac ctcgccctac ctgctgcagc acaaggacaa
      481 ccccgtggac tggtacccgt ggggccccga ggcgctggac gccgcacgcg agcgcgacgt
      541 gccgatcctg ctgtcgatcg gctactcggc ctgccactgg tgccacgtga tggagcgcga
      601 gtcgttcgag gaccccgcga ccgcggcgtt catgaacgac cgcttcgtgt gcatcaagct
      661 ggaccgcgag gagcggcccg acctggacgc catctacatg gaggcgtgcc aggccatgac
      721 cggccacggg ggctggccgc tgaacgtgtt cctgaccccg gaacaggtgc cctactacgc
      781 cgggacctac ttcccgcccg aggaccggca gggcatgccc tcgtggacca ccgtcctgga
      841 cgccgtgcgc gaggcgtggg acgagcgcgc cgacgagatc cgcgccacct cgggcaaggt
      901 ggccgagcgc ctgcgcggcg gggcgggcct ggagcccgcc gccgaggcgc tggacgcgac
      961 ggtgctggag gccgcggtcg agggcctgcg cagcgtctac gacgccgaga acggcggctt
     1021 cggcggcgcc cccaagttcc cgcccgcctc caccgtcgag ttcctgctgg cccggggcga
     1081 gcaggacatg agcctgcaca cgctgcgcgc catggccggc ggcggcatgt acgaccaggt
     1141 cggcggcggg ttcgcgcgct actcggtgga cgcgcgctgg gtggtccccc acttcgagaa
     1201 gatgctctac gacaacgcgc tcctggcccg cgcgtacctg cacggctggc aggcctcggg
     1261 cgacgagctg ctgcggcggg tggccgagga gacgctggac tggatgctga ccgagctgcg
     1321 cgggcccgag ggcggcttca tgtcggcgct ggacgccgac tccgagggcg aggagggcct
     1381 gttctacgtg tggaccccgg accagctgcg cgaggtgctg gggcccgagg acgccgaggc
     1441 ggcggccgcc tacttcggcg tccagccggg cggcaacttc gagggccgga cgatcctgac
     1501 ccgcggcgac gccgagcccg agaacctggc cgacatccgg cgccggctct ccgaggcacg
     1561 cgcgcggcgc gtgtggcccg gcctggacga caagcgcctg acgtcgtgga acgcgctggc
     1621 catcgccgcc ctggccgagg ccggggcggt gctggagcag ccgcgcttcc tggacgccgc
     1681 ccgcgacgcg gcggacttcg tgctgcgcga cctgcgcgac gccgacggcc gcctgctgcg
     1741 cacgtacaag gacggacacg ccaagctgaa cgcctacctg gaggaccacg ccttcctcct
     1801 ggacgcgctc ctggtcctgt accaggccac gttcgagcca cgctggttct ccgaggcccg
     1861 cgcgctggcg gacaccatga tcgagcgctt cgccgacgac gagcgcggcg gcttcttcga
     1921 gacctcggac gaccacgaga agctggtggc gcggcgcaag gacctggagg accaccccat
     1981 cccgtcgggc aactccgccg ccgcgatggg cctgctgcga ttggccgcgc tgaccggcga
     2041 gtacgcgtac gagcagcgtg ccgcgggcgt gctcgcgctg ctccatccca tcgccgccaa
     2101 gcacccgcag gccttctcga acctgctgct ggcggcggac ttccacctgg ccgccgtgcg
     2161 cgaggtggcg ctggccggcg acgacacctc cgagctggag cgtgtggtcc gcgcccggct
     2221 gcgcccccac ctggtcctgg ccggcggcga cccggcaggc gtcccgctgc tgcagggccg
     2281 cgagcccgtc gacggcgagc cggcggccta cgtctgcgag cgcttcgcct gcctgcggcc
     2341 ggtcacgcga ccggctgagc tggaggcttt gctggcgagc ccttagagag cgccgacggc
     2401 caccagacct tgtcgcccat ctgcaggatc agggcgggca ccagcacgct gcgcaccagg
     2461 aaggtgtcca gcagcacgcc gaaggccacg acgaacccga tctcggccag gaacaccagc
     2521 ggcagcgagc ccagcaccat gaaggtgccg gccagcacga tgccggcgct ggtgatcacg
     2581 ccgccggtga ccgcgagcgc cctggtcatg ccctcgcggg tgccgtactt gagcgtctcc
     2641 tcgcgcgccc gcgccacgag gaagatgttg tagtcgaccc ccagcgcgac caggaacacg
     2701 aacgcgaaca gcggtagcga cgggtccgag cccgggaagc cgaagagcac gtcgaaggcg
     2761 aagtagccca cgcccagcgc ggcgacgaag gacaggatca cggtgccgat caggatcagc
     2821 ggcgcgatca cggcgcgcag caggagcacc aggatcagga agaccacgac cagcacgatc
     2881 ggcgggatca cggtggagtc ccacgccgac gcgtcgcgga cgtcgaactc gaccgccgac
     2941 gcgccgccga cgatcgtgcc gtcctgcgcc ccggacacgg cgtcgcggag gggctcgacc
     3001 aggtcgaacg cctcggtgga gtacgggttc ggttcgagcg tcgcctggat cagggtcccc
     3061 tgggcgccct cggccaccgg gggcgtcacg gcctcgacgc cgtcgacccc ttcggcggcc
     3121 gcggtcacgg cggccacgtc gcccccgcgg ggcacgacga tgtcggtcgg gacgctggcc
     3181 cccgacggga agctcctggc cagcaggtcc tggccctcga cggactcgac gtcggtggtg
     3241 tagctgtcgt tggtggtcag gtcggtggag aagaacgcga ggcccgcggt catgatcagc
     3301 agcaggacca ccgagccggt ggccacccgc cgcggcgagg ccgcgacccg cgtcccgacg
     3361 cgcgcccaga acccgtgcgt ggcgtcccgg ggcttctcgt gccggtagcg gcgcagctcg
     3421 tagggcagga acacgtggtc gtgcaggggc cggaagtgcg ccccggtcgc cttgcccagg
     3481 acgaacatga ccaggcggaa gagcagcacg accgggagga acacggccac gaccagcccg
     3541 gccaggacga ccaggccgag cgcgaccggc ttcgagccct cggccacctt gcgggcgatc
     3601 ccgctccgga gcacggcctc ggtcggcgcg gtctccgggg tgtggggcac gaacggccac
     3661 agcgcccggc ggccgaagat cgtcagcagc gccggcagca gcgtgagcat ggacagcgcg
     3721 gcgcaggcca cgcccatcgc cccgatcggc cccaggccgg ccgtgccgtt gaccttggcc
     3781 agcgtcaggc acaacagggc ggcgaccacg gtcgccgccg aggcgaacac tgccgggccc
     3841 gccgagcgca tggcctcggc tatggccacg tgcctgtcgc tggtgtggtg cagctcctcg
     3901 cggtagcggg acacgatcaa cagcgcgtag tcggtccccg cccccaggac gagcacggac
     3961 atgatcgagc tgctctggcc gttgatggtc accccgacct ccgacaggcc gtagccgacc
     4021 gagcgcgaca gcatctcggc gaagatcacc gcgagcaggg gcacgaacag gaacaccggc
     4081 gaccggtaga tcaggatcag caggatgatc accagcgaca ccgcggccag cagcagcgtg
     4141 ccgttgatgc tctcgaacac ctcgatggcg tcggcggcgt agccggcgcc gccggtgatc
     4201 ttgacctcga ggccgccgcc ggggtcggag accttgtcgc gccagtcctg gatcgggtcg
     4261 agcaggctgt ccgactcccc gtcgcccttg aggtatgcgg tgacgatggc ggccttgccg
     4321 tccgccgagc agatcgggcc cacgaacggc tgatagccgc tgggctggcc gcggaccggg
     4381 gtggtcggcc cggcgcagcc cggtgcggcg gccttgccgg tcggccgcgg gccgccctcg
     4441 gaatcgcccc cctgcttgcc gccggaggcc gcggtcgagc cgtcggcgac cacgcccttg
     4501 taacgcttct tcgccagggc cgcgacgtcc ttggtgatgg tctcgcggtc cgatgcggtc
     4561 agccccgact cgcgccggaa gacgatgacc gcgggcgcca gctcaccgct ctggagctcc
     4621 tcggtggcct tgagcgcctt ggtcgactcg gcgtcccccg gcaggaacga ggtggactcg
     4681 ttctcctcgg cgtcggtgaa cttgcccggg aggttggccc cgaaggccac gatcatcccg
     4741 acgagccaga cggcgagcac cacccatttg gcgcgcgacc cgcccgggac ggcgaagatc
     4801 gacgatcggg gtgacttccg ggccggggca ggggagctca tggcgggtgg ttctaccacg
     4861 gtgcccagag gcccaagggg cgggtaaccc cactatgtgt tcacgcgacc gcgacatccg
     4921 ggcgcaccgg cggtctaggc tcgcgccatg cgggggatcc ggcacggcac gcgcgcgctc
     4981 acggccctgg cccttgttgg cgcgctggcc gtcgcggtgc ctgcgacggc ggccacgatc
     5041 aagggctcca agcgcgccga ccgcatcacc ggcaccaagc gcgccgacaa gatcaaggcc
     5101 ggccgcggca acgaccgcgt gcggtccggg cgcggtgccg accgggtcta tggcggccgg
     5161 ggcaacgacc gcatcgacgg cggcagttcc gccgaccgcc tctacggcga gcccggcaac
     5221 gaccgcgtcc gcggccgcgg gggcgacgat cgcgcccggg gcggcgcggg ccgtgaccgg
     5281 ctgaccaccg actccggcga cgacctgctc tacggcgacg cgggcgacga ccgcctcagc
     5341 gccgggttcg gcaacgaccg cgtcttcggc ggcagcggca acgacctgct gttcgccggc
     5401 gatcccggcc gcggcgcgtc accgggcgac gacctggcgg gacaggcggg caacgaccgg
     5461 ctgcgcggcg acaccggcaa cgacgggctg gacggcggcc cgggcaacga ccgcatcgac
     5521 ggcggccgcg gcgaggacga cctgatcggc ggcgagggca acgaccgcat cgacgcgcgc
     5581 gacggcgagc aggacaccgt cgactgcggc gacggccagg acacgctgct ggccgactcg
     5641 ctggaggacg gcgtgaccga gtgcgagacc gtgctggtcc cgccggaccc gcccgaggcc
     5701 tagcggcccg ccctatctgg gcttgtaggc cttcttcacc gaatccggct tggtgaagat
     5761 ctcgtgcggc ttgccgtcgt agacgcggta gcggtagcgg tgcgccacgt cctcgaggcc
     5821 ggccaggtcg tcggcgtgcg cgtcgcgctg gtcggtgatg tcgcccccgc ccatgctgca
     5881 cggctgcgtg gccaccgcgg gcgggtcggg cagcgagggc cggttgaagt ccgggttggc
     5941 ccagtcgaag ctgtggccga tgttgcgcgc gtagcggtgg cggcgggtca ggtggcccag
     6001 cccgaagcgg tggctgatca gcttgaggat cgactcgtgg ccgtacacgc cgtggtccac
     6061 gcgcagctgg tcgcccgtgg tgcgccggcg ggtgtagggc gacaccgtca cggccgggat
     6121 ccggaacccc atctggccga agtcggtgtc caggttggcg gtcgagcgct tgtcggggac
     6181 gcgcggcggc ttgacgtggt cgaagaaccc gccccactcg tcgtagacga tgaacagcgc
     6241 gccgcggcgg tagttgggcg aggccacgaa cgcgttgacc acgtcggcca tgaacgcctg
     6301 ccccagccgg acgtcgccca gcgggtgttc gtcggccgac agcccgtcgc cgcccccgcc
     6361 gtcgccgaag ggcgggtcga tgaacgtgat gttgggcagc gtgcccgccg cgcaatccgc
     6421 gtagtagcgc gaaaccgggt tggcccagcc cagcccgcgc gccccgtaga ccgcggcgaa
     6481 cgggatgtcc gcgtggtagt agcgcgcgct caggccgcgg ccgatggcgc ggtcgaagat
     6541 ggtctcgaac tggttgccgc ccttggcgac gtcgacctgc tcgatgttcg aggtcttgcc
     6601 cgccgacgtg gccgcccact tgtagtagcg gttggggaag gtcggcccca gcagcgagca
     6661 gaagtagcgg tcgtagagcg tgtaggtgct ggccgcctgg tggatgtagc ccagctggcc
     6721 gcggtcgtag tagctcaggg cgaagatgtc gttgccgctg ccctggccca tgaagccgtt
     6781 gcgcagctgg gcgcgcccgg acttccagcc gtggccggga tcgttgtagc cgcacccctt
     6841 ccactgggag ttggccccca ggttcgaggc cggccgcgtg ggcaccatgt tgcccgccgg
     6901 gtcacggaag ctctgcttct gcttgccgtc cgcccccggc gcccagccga agtagtggtc
     6961 gaacgaccgg ttctccatca tcaggaccac gaagtggtcg atctcgacgt tgcgcgggct
     7021 gggcagccgt gccctgcgtg cctcggcgtc ggccgcgtgg tagagcagcg tcgatgcggg
     7081 aaccgcgccc gccatgcccg ccagccccgc cgcgtaggcg gtccgctcca ggaaatcccg
     7141 acgactgagg tcctcctcac gcaccctggg gccaccctac gccaggtgtg ccgcggcgtg
     7201 tagcggttcc gttacctaga ggatcgacat gta
//
LOCUS       DASUPW010000309         2763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_75280, whole genome shotgun sequence.
ACCESSION   DASUPW010000309 DASUPW010000000
VERSION     DASUPW010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75280"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VFY44_06955"
     CDS             complement(<1..265)
                     /locus_tag="VFY44_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5910215.1"
                     /translation="MPILVVDDDRSLRDALRRALTLAGHEVETADNGQQALGGAAGFD
                     PEAIVLDLGLPDMDGLEVCRRLRGAGNRVPVLVLTARDAVEDRI"
     gene            331..1248
                     /locus_tag="VFY44_06960"
     CDS             331..1248
                     /locus_tag="VFY44_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5910216.1"
                     /translation="MPPAATDPQLALRLRGVVKHYGDVKAVDGLDLDLPMGTCVGLLG
                     PNGAGKSTTMKMLTAQAIADQGEIDVLGLRLPEESKPARAQCGVVPQLDNLDTTLTVE
                     ENLVVFSHLYRVPRAERKAAVDRAVGIARLEDRRGAKVDELSGGMRRRLLIARALIHR
                     PRLLLMDEPTVGLDPQVRQELWALIERLRSEGTSILMSTHYIEEAERLADTVTIMSHG
                     RAVATGSPRDLITEHTGPEAIEVYGPPDELAEAERAAKAAGLSTRRTGTSVAVLALDR
                     ATNGDVPDGVRRTANLEDVFVLLTGEEIG"
     gene            1245..2054
                     /locus_tag="VFY44_06965"
     CDS             1245..2054
                     /locus_tag="VFY44_06965"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5910217.1"
                     /translation="MSAAAVTPPPRRLRLKRIERPAVRGVLVREMINFSSYWRASVFS
                     STVEPTIYLLAFGFGFGTLVNEVGGLRYVEFVGTGTVATAVLFSSAFPSMFGTFVKYS
                     FQHTYDAILAAPVDTEELVTGEMLWIAMRAGVFGCAPLLVAMVFGLDPAWGMLLVPFI
                     AFTAGFGWAGFGTAIAGQVNSIDNFSYITSMVLTPLFLVAGTFFPIDGLPEWAQVAAN
                     FNPLYHCVELVRQASFGIDPGSFALHYLAIVAFGLVTWRLAINRMTRKLVT"
     gene            complement(2213..>2763)
                     /locus_tag="VFY44_06970"
     CDS             complement(2213..>2763)
                     /locus_tag="VFY44_06970"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX5910218.1"
                     /translation="SADVVDAVAYSMRAAADVPVGVSTGAWIEPDVLARVAAVHGWSE
                     PDFASVNLGEDGALELMAALVDRGIGIEAGLFGVDDVERLVSGGMSEDLLRVLIEPES
                     HVPAEALETAAAIDRALDDAGVAAPRVAHGAGAATWSVLEWAAAAGHGLRIGLEDTLV
                     MADGSPAEGNAALVEAAAALLE"
BASE COUNT          375 a         1037 c          959 g          392 t
ORIGIN      
        1 cgatgcggtc ctcgaccgcg tcgcgcgccg tcagcaccag cacgggcacg cggttgcccg
       61 cgccgcgcag gcgccggcag acctccaggc cgtccatgtc gggcaggccc aggtccagca
      121 cgatcgcctc ggggtcgaag ccggcggcgc cgcccagggc ctgctggccg ttgtcggccg
      181 tctcgacctc gtggccggca agggtcagcg cgcgtcgcag ggcgtcgcgc agcgaccggt
      241 cgtcgtccac caccaggatt ggcacacctg cattgtggag gtgcggccac ccgtacggcg
      301 tcgctcctac cttgtgctta ccctccagcg gtgccccccg ccgccaccga cccccagctc
      361 gccctgcgcc tacgcggcgt ggtcaagcac tacggcgacg tgaaggccgt ggacggcctg
      421 gacctggacc tgccgatggg cacgtgcgtc gggctgctgg gccccaacgg agcgggcaag
      481 tcgaccacca tgaagatgct gacggcgcag gcgatcgccg accagggcga gatcgacgtg
      541 cttggcctga ggcttcccga ggagtccaag ccggcgcggg cgcagtgcgg cgtcgtgccc
      601 cagctggaca acctggacac cacgctgacg gtggaggaga acctggtcgt gttctcgcac
      661 ttgtaccggg tgccgcgcgc cgagcgcaag gccgccgtgg accgggccgt gggcatcgcg
      721 cgcctggagg accgccgggg cgccaaggtg gacgagctgt cgggcggcat gcgccgccgg
      781 ctgctcatcg cccgcgcgct gatccaccgc ccgcggctgc tgctcatgga cgagcccacg
      841 gtcgggctgg acccccaggt gcgccaggag ctgtgggcgc tgatcgagcg gctgcgcagc
      901 gagggcacct cgatcctgat gtccacgcac tacatcgagg aggccgagcg gctggccgac
      961 accgtgacga tcatgtcgca cggcagggcg gtggccaccg gatcgccgcg cgacctgatc
     1021 accgagcaca ccggcccgga ggcgatcgag gtctacgggc cgcccgacga gctggccgag
     1081 gccgagcgcg cggccaaggc ggcggggctg tccacccgcc gcaccggcac gtcggtcgcg
     1141 gtgctggcgc tggaccgggc caccaacggc gacgtgcccg acggcgtgcg ccggaccgcc
     1201 aacctggagg acgtgttcgt gctgctcacc ggggaggaga tcggatgagc gccgcggcgg
     1261 tgacccctcc cccgcggcgc ctgcgcctga agcgcatcga gcgccccgcc gtccgcgggg
     1321 tgctggtgcg cgagatgatc aacttcagct cctactggcg ggccagcgtg ttctcgtcga
     1381 cggtcgagcc caccatctac ctgctggcct tcggcttcgg cttcggcacg ctggtcaacg
     1441 aggtcggcgg cctgcgctac gtcgagttcg tgggcaccgg caccgtggcc acggcggtgc
     1501 tgttctcgtc ggcgttcccg tccatgttcg ggacgttcgt gaagtactcg ttccagcaca
     1561 cctacgacgc catcctggcc gcgccggtgg acaccgagga gctggtcacc ggcgagatgc
     1621 tgtggatcgc catgcgcgcc ggcgtgttcg gctgcgcccc gctgctggtg gccatggtct
     1681 tcggcctcga tccggcctgg ggcatgctgc tggtcccgtt catcgccttc accgccggct
     1741 tcggctgggc tggcttcggc accgccatcg cgggacaggt gaactcgatc gacaacttct
     1801 cctacatcac ctcgatggtc ctgacgccgc tgttcctggt ggccggcacc ttcttcccca
     1861 tcgacggcct gcccgagtgg gcgcaggtgg ccgccaactt caacccgctc taccactgcg
     1921 tcgagctggt gcgccaggcc tcgttcggga tcgacccggg ctcgttcgcg ctgcactacc
     1981 tggccatcgt ggccttcgga ctggtcacct ggcggctggc gatcaaccgc atgacgcgca
     2041 agctggtgac gtaggggtca gcttctgccg tcagccgcac cagctgatcg gcggcacggc
     2101 ccggctgggt gaggccaggg ggaccgagcc acgccgggaa aactgcgccg ttcatgcgtt
     2161 tggcgcgcca gagccaccca aacgcatgaa gcacgcatcg agaacgaagc cgtcactcca
     2221 gcagcgccgc cgcggcctcc accagcgccg cgttgccctc ggccggcgag ccgtcggcca
     2281 tgaccagcgt gtcctccagg ccgatgcgca ggccgtgccc ggccgccgcg gcccactcca
     2341 gcaccgacca cgtggccgcc ccggcgccgt gggccacccg tggcgcggcc acgccggcat
     2401 cgtccagcgc gcggtcgatg gcggcggccg tctccagggc ctccgcgggg acgtgggact
     2461 ccggctcgat cagcacccgc agcaggtcct cggacatccc gcccgagacc agccgctcca
     2521 cgtcgtccac gccgaacagg ccggcctcga tgccgatccc gcggtccacc agggcggcca
     2581 tcagctccag cgccccgtcc tcgcccaggt tgaccgaggc gaagtccggc tcgctccacc
     2641 cgtgcacggc cgccacccgc gccagcacgt cgggctcgat ccatgcgccc gtgctcacgc
     2701 cgacggggac gtcggcggcc gcccgcatcg agtaggccac cgcgtcgacg acgtccgcgg
     2761 aca
//
LOCUS       DASUPW010000310         2296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1598245, whole genome shotgun sequence.
ACCESSION   DASUPW010000310 DASUPW010000000
VERSION     DASUPW010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598245"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..947
                     /locus_tag="VFY44_06975"
     CDS             51..947
                     /locus_tag="VFY44_06975"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX5910219.1"
                     /translation="MKHAHRFLTLAIAAALLALVPSAAQAAKAKPAKLAVALGDSLST
                     GVQPDAAGKNQATKAGYPYQLAARSGAKLKNFGCGGATSGSLVTSDKKCFEHFGKLPF
                     KNKNRATSQLAAAEKYIRENRDDIAYVTIDIGANDVASCAAGGKLDVKCVTEGIARIK
                     KYVPEIAKRLRKAAGKDVPIAGMTFYNPFLQQWFTNPGLAEATVGIAKTQVNDALTKI
                     YKKQKIKVADVGTAFGTYKPFSQTTTYNGRAGVPVAVANICKYSWMCAAAPRGPNIHA
                     TKAGYTLITDTFQKSIGKAARK"
     gene            complement(960..>2296)
                     /gene="pyk"
                     /locus_tag="VFY44_06980"
     CDS             complement(960..>2296)
                     /gene="pyk"
                     /locus_tag="VFY44_06980"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX5910220.1"
                     /translation="GLDVARLNFAHGTFEQMAQTAERVREASDRAGRNVAILQDLPGP
                     KLRIGPVRDDLAELAVGDTLTLVCGSDEEGDHARLSVTWAGLCEAVQPDEVVYLSDGS
                     VRLRVKAIRGQEIETRVEVGGGVTSRQGLNVPGELSALPAVAEDDLERLRFGETIGVD
                     MVALSFVRRPDEITALREHTDSPLVAKIEKPQAVDNLDAIAHAADCLMVARGDLGIEM
                     DIERVPLVQKQIIRKAGELARPVITATQMLDSMVVSSRPTRAEVADVANAIMDGTDAV
                     MLSQETAVGAHPVAAVKMMDAIARTTEHELPLEHWNHHRVLRQERAEQDPAYTVAYNA
                     CDAAMQLDLAALVVPTLSGRSARIISAHRPRVPIYVLSPDPGVVRRASLMWGVQAESM
                     EPHGVTEELIADASRRVVELGWVKFGERIGITAGLPSGQPGTTSLFQIQAAG"
BASE COUNT          361 a          836 c          778 g          321 t
ORIGIN      
        1 ccggtctagg gttgcccgcg gcaggagcac catttaccgg aggagtcctt ttgaagcatg
       61 cccatcgttt cctgaccctg gccatcgcag ccgcgctgct ggccctggtc ccgtccgccg
      121 cccaggccgc caaggccaag ccggccaagc tcgccgtggc cctcggcgac tcgctctcga
      181 ccggcgtcca gcccgacgcc gccggcaaga accaggccac caaggcgggg tacccgtacc
      241 agctggccgc gcgtagcggg gccaagctca agaacttcgg ctgtggcggc gccaccagcg
      301 gctcgctggt gacctcggac aagaagtgct tcgagcactt cggcaagctg ccgttcaaga
      361 acaagaaccg cgcgacctcc cagctcgcgg cggcggagaa gtacatccgc gagaaccgcg
      421 acgacatcgc gtacgtgacg atcgacatcg gcgccaacga cgtggcctcg tgcgccgccg
      481 ggggcaagct cgacgtcaag tgtgtgaccg agggcatcgc ccggatcaag aagtacgtgc
      541 ccgagatcgc caagcggctg cgcaaggcgg cgggcaagga cgtgccgatc gccggcatga
      601 ccttctacaa cccgttcctg cagcagtggt tcaccaaccc gggcctggcc gaggccaccg
      661 tgggcatcgc caagacccag gtcaacgatg cgctgaccaa gatctacaag aagcagaaga
      721 tcaaggtggc cgacgtgggc accgcgttcg ggacctacaa gccgttcagc cagaccacca
      781 cgtacaacgg gcgtgccggg gtcccggtgg ccgtcgccaa catctgcaag tactcgtgga
      841 tgtgcgctgc cgccccgcgc ggacccaaca tccacgccac gaaggccggc tacacgctga
      901 tcaccgacac gttccagaag tcgatcggca aggccgcgag gaagtaggga ggccggcggc
      961 taaccggccg cctggatctg gaagaggctg gtcgtgccgg gctgtccact gggcagcccg
     1021 gccgtgatcc cgatccgctc gccgaacttg acccacccca gctcgaccac ccggcgcgag
     1081 gcgtcggcga tgagctcctc ggtcacgccg tggggctcca tcgactcggc ctgcacgccc
     1141 cacatgagcg aggcgcggcg caccaccccg gggtcgggcg acagcacgta gatggggacc
     1201 cgcgggcggt gggcggagat gatccgcgcc gagcgcccgg acagcgtggg caccacgagc
     1261 gccgccaggt ccagctgcat ggccgcgtcg caggcgttgt aggccaccgt gtaggccggg
     1321 tcctgctcgg cccgctcctg gcgcagcacg cggtggtggt tccagtgctc cagcggcagc
     1381 tcgtgctcgg tggtgcgggc gatggcgtcc atcatcttca ccgccgcgac cgggtgtgcc
     1441 cccaccgcgg tctcctggga cagcatgacc gcgtcggtgc cgtccatgat cgcgttggcc
     1501 acgtcggcca cctcggcgcg cgtgggccgc gaggacacca ccatcgagtc gagcatctgg
     1561 gtcgccgtga tcacgggccg ggccagctcg ccggccttac ggatgatctg cttctgcacc
     1621 agcggcacgc gctcgatgtc catctcgatg cccaggtcac cgcgggcgac catcaggcag
     1681 tcggcggcgt gcgcgatcgc gtccaggttg tccacggcct ggggcttctc gatcttggcc
     1741 accagcgggc tgtcggtgtg ctcgcgcagc gccgtgatct cgtcggggcg gcgcacgaag
     1801 ctcagcgcga ccatgtcgac cccgatcgtc tcgccgaagc gcagccgctc caggtcgtcc
     1861 tcggccaccg ccggcagcgc ggacagctcc ccggggacgt tcaggccctg gcgtgaggtc
     1921 acgcccccgc ccacctcgac ccgcgtctcg atctcctggc cgcggatggc cttcacgcgc
     1981 aggcgcacgg acccgtcgga gaggtacacc acctcgtccg gctgaacggc ctcgcacagc
     2041 ccggcccagg tgacgctcag gcgcgcgtgg tcgccctcct cgtcgctgcc gcagaccagc
     2101 gtcagggtgt cgcccaccgc cagctcggcc aggtcgtcgc gcacggggcc gatgcgcagc
     2161 ttgggcccgg gcaggtcctg gaggatggcc acgttgcgcc cggcgcggtc ggacgcctcg
     2221 cggacccgct ccgcggtctg ggccatctgc tcgaaggtgc cgtgcgcgaa gttcaggcga
     2281 gcgacgtcca gacccg
//
LOCUS       DASUPW010000311         6300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1322139, whole genome shotgun sequence.
ACCESSION   DASUPW010000311 DASUPW010000000
VERSION     DASUPW010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6300
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322139"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VFY44_06985"
     CDS             complement(<1..384)
                     /locus_tag="VFY44_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX5910221.1"
                     /translation="MGAVHVHEFMTLDGVVDAPAWTAEYGFDPAMGEVLAEVTGASAG
                     ILLGRKTYEMFEPSWSNRTAEDDPGAPFFNDTTKHVVSSTLEDPTWRNSEIVGPYDPE
                     AIRALKESVDGDLYVSGSVQLVRAML"
     gene            465..2639
                     /locus_tag="VFY44_06990"
     CDS             465..2639
                     /locus_tag="VFY44_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEX5910222.1"
                     /translation="MATATVHRQCNLCEAHCGINVEVDGAKVVRITGDPEDEFSRGYI
                     CPKAAALADLYEDPDRLTRPVRRTDGGWEEMEWDEALDFAAEGLRRVRDDHGRDAVAT
                     YLGNPGAHTWSVLSFLALRLVLGTSNNSSATSTDQLPQHLSSAEMFGNLAMFPIPDVD
                     RTQHMLVLGANPAVSNGSIMSAPGVRDRLRGILGRGGTVVVVDPRRTETAKLATEHVQ
                     VRPGGDPYLLLGMLNVLFAGDLVDLGALSEHTDGLDQIRALASEWPPERCAALAGVDA
                     ETIARMAREFAAAETAVAYGRVGVCQQRTGSVTHWLINVLNAVTGNLDRPGGAMFTNP
                     PVDLGLVLKLVSTVGVGGTHRTARQRVSGLPDMNGEFPIAGLAGEITTPGEGQVRAML
                     VYAGNPVLSSPGGDRLGAAMEQLDFCVAIDPHITETSRHADVILPSVSPLERDDLDIV
                     MPAVSVRNHVRFSPAAVPKREGGKEDWEIITGLTRRLGEGVRSRAVAEAVGVGSKLGL
                     SSPARTIELAFAAGPYGLMRKGPFRALTVGKVKKAKHGIDLGPLEPRLPGALATPGKR
                     VRLAPELFVEEARELARLADERDLALADGYDLTLIGRRSLRSNNSWLHNSARLMKGAD
                     RCTALLHPDDAAARGLADGAAVRVASSVGEIELPLEVSDEMRPGVISIPHGFGHGRDG
                     VGWKLAASKPGVSVNDVTDPAVLDRLTGNAAYNAVPVRVAAA"
     gene            complement(2656..3600)
                     /locus_tag="VFY44_06995"
     CDS             complement(2656..3600)
                     /locus_tag="VFY44_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX5910223.1"
                     /translation="MRARPLARWSVLLVLLVPLLPAAPAAAQADPLYCFHATPTIVGT
                     AGDNTIHGTPGNDVIMGLGGRDTIYGGGGNDRICGGAGVDELHGGNDGDNMAADEWGR
                     FADYTPDPRDGADRLYGDGGPDALYDTGSGNDLFVGGAGVDFVKSGGGANRVHGGAGD
                     DYIEGGFGIGDKILGEEGDDQVYDGKGNDWITGGPGDDLLTEVEDFHVVCPGCYNAEV
                     GGGNDRVYGNEGDDTLFSGPGADRLEGGSGADSLGAPVPLYINTEPNGVAREVGNDTL
                     LGQQDADRLTGGPGTDRLDGGSGTDVCSEGESVSNCDR"
     gene            complement(3830..5173)
                     /locus_tag="VFY44_07000"
     CDS             complement(3830..5173)
                     /locus_tag="VFY44_07000"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5910224.1"
                     /translation="MRRSSTGVVPAALGATFLVLVAFTSAITTAGDTAGSFGAGIAWQ
                     TWILSSMSLGLAGALMTCGALADERGARWAMIAGAGGLLAASALAAVAPSIGVFVAAR
                     VLQGVAGAAMLVGALGFIALAVPTGPERTRATGLWGAMVGAGIAAGPVVAGLLAELTD
                     WRLVHWAEAAGALAVLMVARGLPDLRARVRHPIDPPGALTLAASMSALTAGLVSGRSD
                     WTAPVTVALLVAGVALLALFPLVEARRPVPMIEPALFREPLFVASVAGALFLGLSTIA
                     LMSYLPLFAQRVMGASVLGSAVLLTAWSGVSAWVAVETRRLPVGWDGRHRLIAGCAVC
                     AAGLVALAFVGTTSSWAVLVPGMVVVGVGSGLMNAALGRLAVEAVPPERAGMSSGATN
                     TARYLGGAAGVALTIAVATGGGTSSADLVAGWNAAALVAAAFVATGGLVAYVSGR"
     gene            5197..>6300
                     /locus_tag="VFY44_07005"
     CDS             5197..>6300
                     /locus_tag="VFY44_07005"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX5910225.1"
                     /translation="MTVPEPIGVVGAGTMGLGIAQLAAGTGARTLLHDPVPDALAAAP
                     ERMARALERPVAKGRMTAADAEAIVGRVEPVARISGLAPCAVVIEAAPERLELKHELF
                     AELAGVVDAGCVLATNTSSLSVTELAAPVAHPERVVGLHFFNPAPVMRLVEVVAGQAS
                     SAEALATTRAVGEAMGKRVIDARDIAGFLVNRVNRPFSLESLRLLQEGVADAAQIDRI
                     VRLGAGFRMGPFELMDLIGIDTNHAVAEEFHRRTYGEPRYRPSAAAARMVAAGTLGRK
                     TGSGWFAYAEGREHRPPDPDPPPAGGGDGRAVPVAGAGAVADALRAALAGAGFDPDPA
                     TAEPWLTIGCDGPAGQGHRLVLVDRASLHHMDPG"
BASE COUNT          855 a         2356 c         2287 g          802 t
ORIGIN      
        1 cagcatcgcc cggaccagct ggacgctgcc gctgacgtac aggtcgccgt ccacgctctc
       61 cttgagcgcg cggatggcct ccgggtcgta gggacccacg atctccgagt tgcgccacgt
      121 cggatcctcg agcgtcgacg acacgacgtg cttggtggtg tcgttgaaga acggcgcgcc
      181 cgggtcgtcc tcggccgtgc ggttcgacca cgacggctcg aacatctcgt aggtcttgcg
      241 gccgagcagg atgccggcgc tggcgcccgt gacctcggcc aggacctctc ccatcgcggg
      301 gtcgaagccg tactcggcgg tccacgccgg cgcgtcgacc acgccgtcga gggtcatgaa
      361 ctcgtgtacg tgaactgctc ccatttccta cctcctgatc gatggcgtcc tgccactgcg
      421 tcgaaccggg cgcaccgaaa tcgacagggt gcgacaatcg cgccatggcg accgcgaccg
      481 tgcaccgcca gtgcaacctg tgcgaggccc actgcggcat caacgtagag gtggacggcg
      541 cgaaggtggt gcggatcacc ggcgaccccg aggacgagtt ctcgcgcggg tacatctgcc
      601 ccaaggccgc ggcgctggcc gacctgtacg aggaccccga ccgcctgacg aggccggtgc
      661 gccgcaccga cggcggctgg gaggagatgg agtgggacga ggcgctggac ttcgccgcgg
      721 agggactgcg gcgggtacgc gacgaccacg gccgggacgc tgtggccacc tacctgggca
      781 accccggcgc gcacacgtgg tcggtgctct cgttcctggc cctccgcctg gtgctgggca
      841 ccagcaacaa ctcctcggcc acgtcgaccg accagctgcc ccagcacctg agctcggccg
      901 agatgttcgg caacctggcc atgttcccga tccccgacgt ggaccgcacg cagcacatgc
      961 tcgtgctggg cgccaacccg gccgtgtcca acggctcgat catgtcggcg ccgggggtgc
     1021 gtgaccgcct gcgggggatc ctgggccggg gcggcacggt tgtggtcgtg gacccgcgcc
     1081 ggaccgagac cgccaagctg gccaccgagc acgtgcaggt gcgccccggc ggcgacccct
     1141 acctgctcct gggcatgctc aacgtgctgt tcgccggcga cctcgtggac ctgggcgcgc
     1201 tgtccgagca caccgacggc ctggaccaga tccgcgcgct ggcgtcggag tggccgcccg
     1261 agcgctgcgc cgcgctggcc ggcgtcgacg ccgagaccat cgcccggatg gcgcgcgagt
     1321 tcgctgccgc cgagaccgcc gtggcctacg gccgggtggg cgtgtgccag cagcggacgg
     1381 gatcggtcac gcactggctg atcaacgtgc tgaacgcggt gaccggaaac ctggaccgcc
     1441 cgggcggggc catgttcacc aacccgccgg tggacctggg gctggtgctc aagctggtgt
     1501 ccacggtggg cgtggggggc acgcaccgca cggcccgcca gcgcgtcagc ggcctgcccg
     1561 acatgaacgg cgagttcccc atcgcggggc tggccggcga gatcaccacg cccggcgagg
     1621 gccaggtgcg cgccatgctc gtgtacgccg gcaacccggt gctctcgtcc cccggcggcg
     1681 accggctggg cgcggccatg gagcagctgg acttctgcgt ggccatcgac ccgcacatca
     1741 ccgagaccag ccgccacgcc gacgtgatcc tgccgtcggt gtccccgctg gagcgcgacg
     1801 acctggacat cgtcatgccg gcggtgtcgg tgcgcaacca cgtgcgcttc agcccggccg
     1861 cggtgcccaa gcgcgagggc ggcaaggagg actgggagat catcaccggc ctgacccggc
     1921 ggctgggcga gggtgtcagg agccgcgccg tggccgaggc cgtgggcgtc ggctcgaagc
     1981 tcgggctgtc cagcccggcg cgcacgatcg agctggcgtt cgccgccggc ccctacggcc
     2041 tgatgcgcaa gggcccgttc cgggcgctga ccgtgggcaa ggtcaagaag gccaagcacg
     2101 gcatcgacct gggaccgctc gagccgcgcc tgcccggggc gctggccacc cccggcaagc
     2161 gtgtgcgcct ggcccccgag ttgttcgtgg aggaagcgcg cgagctggcc cggctggcgg
     2221 acgagcgcga cctggcgctg gccgacggct acgacctgac gctgatcggg cgccggtcgc
     2281 tgcgcagcaa caactcatgg ctgcacaaca gcgcgcggct gatgaagggc gccgaccgct
     2341 gcaccgcgct gctgcacccc gacgacgccg ccgcgcgcgg cctggccgac ggcgcagccg
     2401 tgcgcgtggc ctcgagcgtg ggcgagatcg agctcccgct ggaggtcagc gacgagatgc
     2461 gtcccggcgt gatctccatc ccgcacgggt tcggccacgg tcgcgacggc gtgggctgga
     2521 agctggcggc atcgaagccg ggcgtcagcg tcaacgacgt gaccgacccc gccgtgctgg
     2581 accgcctgac cggtaacgcc gcctacaacg cggtgccggt gcgggtggcc gcggcctgag
     2641 ctccggcgct catggtcacc ggtcgcagtt gctgaccgac tcgccctcgc tgcacacgtc
     2701 cgtgcccgag cctccgtcca ggcggtccgt gcccggtccc ccggtcagcc ggtcggcgtc
     2761 ctgctggccc agcagcgtgt cgttgccgac ctcgcgcgcg acgccgttgg gctcggtgtt
     2821 gatgtagagc ggtaccgggg ccccgaggct gtcggcgccc gagccgccct cgaggcggtc
     2881 ggcgcccggg ccgctgaaca gcgtgtcatc gccctcgttg ccgtacaccc ggtcgttgcc
     2941 gccacctacc tcggcgttgt agcaacccgg gcacaccacg tggaagtcct cgacctccgt
     3001 gagaaggtcg tctccggggc ctccggtgat ccagtcgttg cccttgccgt cgtacacctg
     3061 gtcgtcgccc tcctcgccca ggatcttgtc gccgatcccg aacccaccct cgatgtagtc
     3121 gtccccggct cccccgtgca cgcggttcgc gcccccgccc gacttgacga agtcgacgcc
     3181 ggcgcccccg acgaacaggt cgttgccgct accggtgtcg tacagcgcgt ccgggccccc
     3241 gtcgccgtac agccggtcgg ccccgtcgcg ggggtcgggc gtgtagtccg cgaaccggcc
     3301 ccactcgtcg gccgccatgt tgtcgccgtc gttgccccca tggagctcgt cgacccccgc
     3361 gccgccgcag atccggtcgt tcccgccgcc accgtagatc gtgtccctgc cgcccaggcc
     3421 catgatcacg tcattgcccg gcgtcccgtg gatcgtgttg tcgcccgcgg tcccgacgat
     3481 ggtcggcgtc gcgtggaagc agtacagggg atctgcctgg gcggcggccg gcgcggccgg
     3541 gagcagaggt accaggagga cgagcagtac ggaccagcga gcgagcgggc gggcacgcat
     3601 ccttcgagta tgcgcccgta tcggcgcagc cgcaagcggc cgttcagtcc tccgagatgg
     3661 gcgcctcgag tgtgccgccg cacgcgggac acgtggcgtc ctcatcggtg agcaacgcgg
     3721 cgctgtacca gcgcttttcg cattccgagc accggcgctc ccctggctgt gcggcgtccc
     3781 cctgattcat agacgggatc gttcccgtac cacgcgggga caaaacgcgt cagcggccgc
     3841 tgacgtaggc cacgagacct ccggtggcca cgaacgccgc ggccaccagc gccgccgcgt
     3901 tccacccggc caccagatcg gcggaggacg tgccgccgcc ggtcgcgacc gcgatcgtga
     3961 gcgccacccc ggcggcgccg ccgaggtagc gagcggtgtt ggtggctccg ctggacatgc
     4021 cggcgcgctc gggcgggacg gcttcgacgg ccaggcggcc cagcgccgcg ttcatgagcc
     4081 cgctgcccac accgaccacg accatcccgg gcacgaggac cgcccacgac gacgtggtcc
     4141 cgacgaacgc cagggccacg aggccggcgg cgcagaccgc gcagccggcg atcagccggt
     4201 ggcgcccgtc ccagcccacg ggcaggcgcc gggtctccac cgccacccag gccgacaccc
     4261 ccgaccaggc ggtcaggagg acggcgctgc ccaggaccga ggcgcccatc acgcgctgcg
     4321 cgaacagcgg cagatagctc atcagcgcga tggtcgacag gcccaggaac agcgcgccgg
     4381 ccaccgatgc cacgaacagc ggctcgcgga acagcgcggg ctcgatcatg ggcaccggcc
     4441 gccgggcctc gaccagcggg aacagcgcca gcagcgccac gccggccacc agcagcgcca
     4501 cggtcacggg agccgtccag tccgagcgcc cgctgaccag cccggcggtg agcgccgaca
     4561 tcgaggccgc cagcgtgagc gcacccggtg gatcgatggg gtggcgcacc cgggcgcgca
     4621 ggtcgggcag cccgcgcgcc accatcagca cggccagggc cccggccgcc tccgcccagt
     4681 gcaccagccg ccagtcggtc agctccgcca gcaggcccgc gaccacgggc ccggcggcga
     4741 tcccggcgcc gaccatggct ccccacagcc cggtggcccg ggtgcgctcc ggcccggtgg
     4801 gcacggccag cgcgatgaag cccagcgcgc ccaccagcat cgccgcaccc gcgaccccct
     4861 ggagcacccg ggcggccacg aacacgccga tgctcggggc caccgcggcc agcgcggacg
     4921 cggccagcag gccgccggcc ccggcgatca tggcccagcg ggcgccgcgc tcgtccgcca
     4981 gggcgccgca ggtcatcagg gccccggcca ggcccaggct catgctggac aggatccacg
     5041 tctgccaggc gatcccggcg ccgaacgagc ccgcggtgtc tcccgccgtg gtgatggcgc
     5101 tggtgaacgc gaccagaacc aggaacgtgg cgcccagggc ggccggcacg acgccggtgg
     5161 aggagcgcct catcggctga gaataggctc cgcgccatga cggtccccga gcccatcggc
     5221 gtggtcggcg ccggcacgat ggggctgggc atcgcgcagc tggccgccgg caccggggcg
     5281 cgcacgctgc tccacgaccc ggtgccggac gcgctggccg ccgcgccgga gcggatggcc
     5341 cgggcgctgg agcggccggt ggccaagggg cggatgaccg ccgccgatgc cgaggccatc
     5401 gtcgggcgcg tggagccggt cgcccggatc tcggggctgg cgccgtgcgc cgtcgtcatc
     5461 gaggcggctc cggagcggtt ggagctcaag cacgagctgt tcgccgaact ggcgggcgtc
     5521 gtcgacgccg gctgcgtcct ggccaccaac acctcgtcgc tctcggtcac cgagctggcg
     5581 gctccggtgg cccatcccga gcgcgtggtc gggctgcact tcttcaaccc cgccccggtc
     5641 atgcggctgg tcgaggtggt ggcggggcag gcctcgtccg cagaggcgct ggccacgacc
     5701 cgggcggtgg gggaggccat gggcaagcgc gtgatcgacg cccgcgacat cgccggcttc
     5761 ctggtcaacc gggtcaaccg cccgttctcg ctcgagtcgc tccgcctgct ccaggagggc
     5821 gttgcggacg cggcgcagat cgaccgcatc gtgcggctgg gcgcgggctt tcgcatgggc
     5881 ccgttcgagc tgatggacct gatcgggatc gacacaaacc acgcggtggc cgaggagttc
     5941 catcgccgca cctacggcga gccgcgctac cggccctcgg cggcggcggc gcgcatggtg
     6001 gcggcgggca cgctggggcg caagaccggg agcggctggt tcgcctacgc ggaggggcgc
     6061 gagcaccggc cgcccgaccc cgacccacct ccggccggcg gcggcgacgg ccgggccgtg
     6121 ccggtggcag gcgcgggcgc cgtggccgac gctctgcggg cggccctggc cggtgccggg
     6181 ttcgatccgg accccgccac ggcagagccg tggctgacga tcggctgcga cgggccggcg
     6241 ggtcaggggc accggctggt gctggtcgac cgcgcttcgc tgcaccacat ggatccgggc
//
LOCUS       DASUPW010000312         4002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1599496, whole genome shotgun sequence.
ACCESSION   DASUPW010000312 DASUPW010000000
VERSION     DASUPW010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599496"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VFY44_07010"
     CDS             complement(<1..198)
                     /locus_tag="VFY44_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910226.1"
                     /translation="MVLILIQQGLVLVGYTAVIIAAAVLCLPAIIVLVASGAGVAGIV
                     VAAVTAVVVLPAALAAAGAVGT"
     gene            complement(371..802)
                     /gene="atpC"
                     /locus_tag="VFY44_07015"
     CDS             complement(371..802)
                     /gene="atpC"
                     /locus_tag="VFY44_07015"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEX5910227.1"
                     /translation="MARSQFPVEVLTPEGQVFEGEVEMVSTRTGTGSLGVLANHAPLM
                     AMLEPCELKLYESGSESPGSASYESYAQGEGYLQVVDNSALIIVEDAIKPEDLDKSEL
                     ESKLKDAESARDDAEEDSEELRRAEREIKRAKAFLEINSGS"
     gene            complement(802..2268)
                     /gene="atpD"
                     /locus_tag="VFY44_07020"
     CDS             complement(802..2268)
                     /gene="atpD"
                     /locus_tag="VFY44_07020"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX5910228.1"
                     /translation="MEASTAEKPDEKQTEDKVGRIEEITGVVIEASFDGHLPEIYNAI
                     ECEIPSQEEDGDPVKLVCEVQQHLGDDRVRAVAMDATDGLSRGTEVRDTGGPITVPVG
                     ENTLGRIFNLLGDVIDEGGEVETEERWPIHRPAPDVEDLTPTNEIFETGIKVVDLLAP
                     YAQGGKVGLFGGAGVGKTVLIQELINNLAKEHGGLSAFCGVGERSREGNDLWNEMKES
                     GVIDKTMLVFGQMNEPPGARMRVALSGLTMAEYFREQGQDVLLFIDNIFRFVQAGSEV
                     SALLGRMPSQVGYQPTLETEMGQLQERITSTREGSVTSIQAIYVPADDLTDPAPASVF
                     AHLNATTTLSRSISEKGIYPAVDPLDSTSTILKPDVVGEEHYAVATQVQEILQRYKEL
                     QEIIAILGIDELSDDDKQTVGRARRVERFLSQPFHVAEQFTGTPGTYVSIEDTVRSFK
                     EIVDGDHDDRPERAFYMKGSIAEVTDEGKSSKSDEDDE"
     gene            complement(2320..3234)
                     /locus_tag="VFY44_07025"
     CDS             complement(2320..3234)
                     /locus_tag="VFY44_07025"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004145.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX5910229.1"
                     /translation="MATQHDVKNRIGSVKNIQKITRAMEMVAAARLRRAEQRIEHLRP
                     YARAIRKMTRRTVEAVEKIPSLPLLEAHESEDKVGILLITGDRGLAGAFNSQILREGN
                     RRALALEQEGKEIVWYASGRRGVSSLEFRGRDVAGQWAGFTDRPAFSDARDIAQDIAA
                     QYIDGKVDRVEMIYNGYVSAMTQNVTTDVLLPLAAVEDILEDDDDEEEESSEDRDSGH
                     KTLWIYEPDPEEILQRLVPDYIEISIYRALLESTASEHGSRMTAMRSAQENASDLISD
                     LTLEMNRQRQAEITQEIMEVVAGAEGLS"
     gene            complement(3238..>4002)
                     /locus_tag="VFY44_07030"
     CDS             complement(3238..>4002)
                     /locus_tag="VFY44_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910230.1"
                     /translation="ELGGGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLEGDLFR
                     SGVRPAINVGISVSRVGGDAQPKAMKKVAGKLRLDLSQYRELEAFAQFGSELDADTQA
                     TLSRGERLVELLNQDELKPWKPADQVAAIYSGTGGYLDRIKTERIREFHEQLLSRLHS
                     EESDLMETLDKGDWDDDIEKKLGDAIAESLDDFGPDFHEDGTPLEEGESDRILSEEER
                     EKEGRTSGSSQDEGSDDDDSDSEDSADEEEEAAAPA"
BASE COUNT          534 a         1389 c         1368 g          711 t
ORIGIN      
        1 ggtgcccacg gcgcccgccg ccgccagcgc cgcgggtagg accaccaccg ccgtgacggc
       61 cgcgacgacg atgccggcga cgcccgcgcc ggaggccacg agaacgatga tcgccgggag
      121 acacagcacc gcggcggcga tgatgaccgc ggtgtagccg accagcacca gcccctgctg
      181 gatcaggatc agcaccaccg acggggccag ctcggacgcg tcgagcgcgg gtaggtcccc
      241 gcccgcgatc tggcggcccc agatcaggga ccacagcggg tcgtagccgg cgtagcccgg
      301 cccgtaggcc agccgcgccg ccagtgccag ccccccgatg accgctgccg cgccggcagc
      361 ggaggcggcc ctacgaaccg ctgttgatct ccaggaaggc cttggcgcgc ttgatctcgc
      421 gctctgcgcg ccggagctcc tcggagtcct cttccgcgtc gtcgcgggcg gactcggcgt
      481 ccttgagctt ggactccagc tcgctcttgt ccaggtcctc gggcttgatc gcgtcctcga
      541 cgatgatcag cgccgagttg tcgaccacct ggaggtagcc ctcgccctgg gcgtagctct
      601 cgtagctggc gctgccgggg ctctcggacc cggactcgta cagcttgagc tcgcacggct
      661 cgagcatggc catcagggga gcgtggttgg ccagcacgcc cagcgagccg gtgccggtgc
      721 gggtggagac catctccacc tcaccctcga acacctggcc ctcgggcgtg aggacctcca
      781 cggggaactg cgaacgggcc actactcgtc gtcctcgtca ctcttcgagg acttgccctc
      841 gtcggtgacc tcggcgatcg agcccttcat gtagaaggcg cgctccgggc ggtcgtcgtg
      901 gtcgccgtcg acgatctcct tgaacgagcg gacggtgtcc tcgatcgaca cgtaggtgcc
      961 gggcgtgccg gtgaactgct ccgccacgtg gaacggctgg ctcaggaagc gctcgacgcg
     1021 gcgggcgcgg ccgaccgtct gcttgtcgtc gtcggacagc tcgtcgatgc ccaggatggc
     1081 gatgatctcc tggagctcct tgtagcgctg caggatctcc tggacctggg tggccaccgc
     1141 gtagtgctcc tcgcccacga cgtcgggctt gaggatcgtg gacgtggagt ccagcgggtc
     1201 gacggccggg tagatgccct tctcggagat cgaccgcgac agcgtggtgg tggcgttcag
     1261 gtgcgcgaac accgacgccg gggccgggtc ggtcaggtcg tcggcgggca cgtagatggc
     1321 ctggatcgag gtgaccgagc cctcgcgggt cgacgtgatc cgctcctgca gctggcccat
     1381 ctcggtctcc agcgtgggct ggtagcccac ctgggacggc atccggccca ggagcgcgga
     1441 cacctcggag ccggcctgca cgaaccggaa gatgttgtcg atgaacagca gcacgtcctg
     1501 gccctgctca cggaagtact cggccatggt caggccggac agggccacgc gcatgcgggc
     1561 tccgggcggc tcgttcatct ggccgaacac gagcatcgtc ttgtcgatga cgcccgattc
     1621 cttcatctcg ttccagaggt cgttgccctc gcgggagcgc tcgcccacgc cgcagaaggc
     1681 ggacaggccg ccgtgctcct tggccaggtt gttgatcagc tcctggatca gcacggtctt
     1741 gcccacgccg gcgccgccga acaggccgac cttgccgccc tgggcgtacg gggccagcag
     1801 gtccacgacc ttgatgccgg tctcgaagat ctcgttggtc ggcgtcaggt cctcgacgtc
     1861 gggcgccggg cggtggatcg gccaccgctc ctcggtctcg acctcgccgc cctcgtcgat
     1921 gacgtcaccc agcaggttga agatccggcc gagcgtgttc tcgccgacgg gcacggtgat
     1981 cgggccgccg gtgtcgcgca cctcggtgcc gcggctgagg ccgtcggtgg cgtccatggc
     2041 gaccgcgcgc acgcggtcgt cgcccaggtg ctgctggacc tcgcacacga gcttgacggg
     2101 gtcgccgtcc tcctcctgtg aggggatctc gcactcgatg gcgttgtaga tctcgggcag
     2161 gtgcccgtcg aacgaggcct cgatgaccac gccggtgatc tcctcgatgc ggccgacctt
     2221 gtcctcggtc tgcttctcgt caggcttctc tgcggtgctg gcttccatag tcaagctcct
     2281 ttggcgaagc agccggcgtt gccggctgcg cacgaggttc tagctgaggc cttctgctcc
     2341 ggccacgact tccatgattt cttgggtgat ctccgcctgg cgctggcggt tcatctcgag
     2401 cgtcagatcc gagatcagat cgctcgcgtt ctcctgtgcg ctgcgcatgg cggtcatgcg
     2461 cgagccgtgc tccgaggcgg tggactccag caacgcccgg tagatcgaga tctcgatgta
     2521 gtccggcacg agccgctgga ggatctcctc cgggtcgggc tcgtagatcc acaaggtctt
     2581 gtggccgctg tcgcggtcct cggaggactc ctcttcctcg tcgtcgtcgt cctcgaggat
     2641 gtcctcgacg gccgcgagcg gcagcagcac gtcggtggtc acgttctggg tcatcgccga
     2701 gacgtagccg ttgtagatca tctccacgcg gtcgaccttg ccgtcgatgt actgcgcggc
     2761 gatgtcctgg gcgatgtcac gggcgtcgga gaacgcgggg cggtcggtga agccggccca
     2821 ctggccggcc acgtcgcggc cgcggaactc cagcgacgag acgccgcggc gcccggacgc
     2881 gtaccagacg atctccttgc cctcctgctc cagggccagc gcgcggcggt tgccctcgcg
     2941 caggatctgg gagttgaagg ccccggccag gccacggtcc ccggtgatca gcaggatgcc
     3001 gaccttgtcc tcggactcgt gggcctccag cagcgggagg ctcgggatct tctccacggc
     3061 ctccacggtg cggcgggtca tcttccggat cgcgcgggcg tacgggcgca ggtgctcgat
     3121 gcgctgctcg gcccggcgca gccgcgccgc ggccaccatc tccatggcgc gcgtgatctt
     3181 ctggatgttc ttgaccgaac cgatccggtt cttgacgtcg tgctgggtgg ccatgggcta
     3241 ggcgggagcc gccgcctcct cttcctcgtc ggccgagtcc tcggagtccg agtcgtcgtc
     3301 gtcggagccc tcgtcctggc tcgaaccgga ggtgcgccct tccttctcgc gctcctcctc
     3361 gctcaggatc cggtccgact cgccctcctc cagcggcgtg ccgtcctcgt ggaagtccgg
     3421 gccgaagtcg tcgagggact cggcgatggc gtcaccgagc ttcttctcga tgtcgtcgtc
     3481 ccagtcgccc ttgtccagcg tctccatgag gtccgactcc tccgagtgca gccgcgacag
     3541 cagctgctcg tggaactcgc ggatgcgctc ggtcttgatc cggtccaggt agccgccggt
     3601 gccggagtag atggccgcca cctggtcggc cggcttccac ggcttcagct cgtcctggtt
     3661 cagcagctcg accaggcgct cgcctcggct cagcgtggcc tgcgtgtcgg cgtccagctc
     3721 ggagccgaac tgggcgaagg cctccagctc gcggtactgc gagaggtcca ggcgcagctt
     3781 gccggccacc ttcttcatgg ccttgggctg ggcgtcgccg ccgacgcgcg acaccgagat
     3841 gcccacgttg atggccggtc gaacaccgga gcggaacagg tcaccctcga ggaagatctg
     3901 cccgtcggtg atcgagatga cgttggtcgg gatgtaggcc gacacgtcgc ccgcctgcgt
     3961 ctcgatgatc ggcagcgccg tcagcgagcc gccgccgagc tc
//
LOCUS       DASUPW010000313         3311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1322452, whole genome shotgun sequence.
ACCESSION   DASUPW010000313 DASUPW010000000
VERSION     DASUPW010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322452"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VFY44_07035"
     CDS             <1..791
                     /locus_tag="VFY44_07035"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5910231.1"
                     /translation="PETSPQDKATSALVDRLRDRTIPAATRDGDLNVYVGGLTASFED
                     FSSKISSRLPLFIGIVIGLSILLLIAVFRSIWVPLASAVFNLLSIGAAYGVVVAVFQW
                     GWGASLLGAAEGTPIVSFIPLMMFAILFGLSMDYNVFLLSRIREAYFEGDSPKESVAH
                     GLSRIAKVILTAGLIMASVFLGFATGDDTIVKMFGVGLGAAILIDVLIVRMVVSPALM
                     ALLGDKAWWIPGWLDRALPNVSLEGGHEDRAGKVEERERQPAGV"
     gene            833..1303
                     /locus_tag="VFY44_07040"
     CDS             833..1303
                     /locus_tag="VFY44_07040"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5910232.1"
                     /translation="MARSTFSDLDCSIARTLDIVGSRWTLLVLRDLFGGITRFDAIQA
                     DLGISRKVLAERLSSLEQAGVVERRAYQDNPPRYDYVLTEKGSELGAVLLAMIAWGDR
                     WEAGPDGPPALFRHEQCGQVTHAVAACSECGEPLDRGNITRVTPPGSRSEPAAA"
     gene            complement(1263..>3311)
                     /locus_tag="VFY44_07045"
     CDS             complement(1263..>3311)
                     /locus_tag="VFY44_07045"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5910233.1"
                     /translation="VFGHDVERHLKAAGFTDSASQSERATEQLRDQLGYDATPGIVLL
                     VRARDHGRLDVRSPAVRREVARLARELRRTEHVGRVVDPLRDRREGRALIARDGRSLV
                     LSGHLSTQDIEDEGGDAAEEAKRRLAPSTLDVRMGGFAPSFNEVNDQTRKDLTKAELI
                     AFPILALLLLVVFRGVVAAAIPLVIGVFSILGAFLMLRLMAGIVDTSLFALNIATALS
                     LGLAVDYALLMVSRYREEIDVAGATREAHRKMVMTAGRTALFSGFTVAVAMASLVLFP
                     QRFLYSVGVAGAVVGLVSAVIAVLVVPAALALLGTRVNALSVRSGPAVSDDSGGWYRL
                     AWGVMKRPALVALASAALLLAASAPLLSTVLTGPSAEAVPPGQPSYGANEGIEAAYPR
                     SVSEPITVAVRGDQDPRELAALSRRIRGLEGIAGGTPFARAGPGLAYATFGPDRPALD
                     AVPQEAVDRIRELRPAGGGDILVTGNTARFMDEKSSLVDHLPLVAAVVALSTFVLLFM
                     LTGSVVIPVKTLLMNTLTLAATLGLLVLAFQEGWLDGPTDYIGPAAVEVTSLVFLFAV
                     IFGLATDYAVLVVARIKEQHDLGVPNEEAVAVGIGRTGRVITAAAVMIAVVFLAFGVS
                     SVFFMKQIAVGQAVGVLLDATVVRALLVPSLMALLGDWNWWAPAPLRRLQARFGFQEA
                     "
BASE COUNT          484 a         1202 c         1177 g          448 t
ORIGIN      
        1 tccccgagac ctcgccgcag gacaaggcga cgtcggcgct ggtggaccgg ctgcgcgacc
       61 ggaccattcc ggcggcgaca cgggacggcg acctgaacgt gtacgtcggc ggcctgaccg
      121 cctcgttcga ggacttctcg agcaagattt ccagccgcct gccgctgttc atcggcatcg
      181 tgatcgggct ctcgatcctg ctgctgatcg ccgtgttccg ctcgatctgg gtgccgctgg
      241 cctcggcggt gttcaacctg ctgtccatcg gcgccgccta cggcgtggtg gtcgcggtgt
      301 tccagtgggg ctggggcgcg tcgctcctgg gcgccgccga gggcacgccg atcgtctcgt
      361 tcatccccct gatgatgttc gcgatcctgt tcgggttgtc catggactac aacgtcttcc
      421 tgctctcacg gatcagggag gcgtacttcg agggcgactc gcccaaggag agcgtggccc
      481 acggcctgtc gcggatcgcc aaggtcatcc tgaccgccgg cctgatcatg gcctcggtgt
      541 tcctgggctt cgcgaccggc gacgacacga tcgtgaagat gttcggggtc ggcctgggcg
      601 cggcgatcct gatcgacgtg ctgatcgtgc gcatggtggt ctcccccgcg ctgatggcgc
      661 tgctgggcga caaggcctgg tggattcccg gctggctgga ccgggcgctg cccaacgtgt
      721 cgctcgaggg cggccacgag gatcgcgcgg gcaaggtcga ggagcgcgag cgccagccgg
      781 cgggcgtctg actcagttcc caacgggaac gcgctcgtct acgatcagca ccgtggcccg
      841 gtccaccttc agcgacctcg actgctcgat agcccggacc ctcgacatcg tcgggtcgcg
      901 ctggaccctg ctggtgctgc gcgacctgtt cgggggcatc acccggttcg acgccatcca
      961 ggcggacctg ggcatctcgc gcaaggtcct ggccgagcgg ctgtcgtcgc tggagcaggc
     1021 gggcgtggtc gagcgccgcg cctaccagga caacccgccc cgctacgact acgtgctgac
     1081 cgagaagggc tcggagctgg gcgccgtgct gctggcgatg atcgcgtggg gcgaccgctg
     1141 ggaagcgggc cccgacggtc ccccggcgct gttccgccac gagcagtgcg gccaggtcac
     1201 ccacgccgtc gccgcgtgct cggagtgcgg ggagccgctg gaccgcggca acatcacgcg
     1261 cgtcacgcct cctggaagcc gaagcgagcc tgcagccgcc tgagcggcgc cggcgcccac
     1321 cagttccagt cgcccagcag cgccatcagc gagggcacca ggagcgctcg cacgacggtg
     1381 gcgtccagca agacgccgac ggcctggccg accgcgatct gcttcatgaa gaacaccgag
     1441 ctgacgccga aggccaggaa gacgacggcg atcatgaccg ccgccgcggt gatcacccgg
     1501 ccggtgcggc cgatgcccac ggccaccgcc tcctcgttcg ggacgcccag gtcgtgctgc
     1561 tccttgatcc gggccaccac gagcacggcg tagtcggtgg ccaggccgaa gatcacggcg
     1621 aacaggaaca ccaggctggt cacctcgacc gccgccggcc cgatgtagtc cgtcggaccg
     1681 tccagccatc cctcctggaa ggccagcacc aggaggccca gcgtggccgc cagcgtcagg
     1741 gtgttcatca gcagcgtctt gaccgggatc accaccgagc cggtgagcat gaacagcagc
     1801 acgaacgtcg acagcgccac cacggcggcc accagcggca ggtggtcgac caggctcgac
     1861 ttctcgtcca tgaagcgggc ggtgttgccg gtgaccagga tgtcgccgcc gcccgccggc
     1921 cgcagctccc ggatgcggtc cacggcctcc tggggcacgg cgtccagcgc ggggcggtcg
     1981 gggccgaagg tggcgtaggc caggcccggg cccgcgcggg cgaacggggt gccgccggcg
     2041 atgccctcca ggccccggat gcggcgcgag agcgccgcca gctcccgcgg gtcctggtcg
     2101 ccacggaccg caacggtgat gggctcgctg acgctgcgcg gataggccgc ctcgatgccc
     2161 tcgttggcgc cgtaggaggg ctggccgggc ggcacggcct cggcgctggg gccggtcagg
     2221 acggtcgaca gcagcggcgc cgacgccgcc agcaggagcg cggcgctggc cagggccacc
     2281 agcgccgggc gcttcatcac cccccacgcc agccggtacc agccgccgga gtcgtcggac
     2341 acggccgggc cgctgcgcac cgacagcgcg ttcacgcgcg tgcccaggag cgccagcgcc
     2401 gccggcacca cgagcacggc gatcaccgcc gacaccaggc ccaccaccgc gccggccacc
     2461 ccgaccgagt acaggaaccg ctgcgggaac aggaccagcg atgccatggc caccgccacc
     2521 gtgaagccgg agaacagggc cgtgcggccg gcggtcatga ccatcttgcg gtgggcctcg
     2581 cgggtggcgc cggccacgtc gatctcctcg cggtaacgcg agaccatcag cagcgcgtag
     2641 tccaccgcca ggcccaggct cagcgccgtg gcgatgttca gcgcgaacag cgacgtgtcc
     2701 acgatcccgg ccatcaggcg cagcatcagg aaggcgccga ggatcgagaa cacgccgatc
     2761 accagcggga tggccgccgc caccacgccg cggaagacca ccaggagcag cagcgccagg
     2821 atcgggaagg cgatcagctc ggccttggtc aggtccttgc gtgtctggtc gttgacctcg
     2881 ttgaacgagg gcgcgaaccc gcccatgcgc acgtccaggg tgctcggcgc caggcgccgc
     2941 ttggcctcct cggccgcgtc gccgccctcg tcctcgatgt cctgggtcga caggtggccc
     3001 gaaagcacca gcgagcgccc gtcgcgggcg atcagggccc ggccctcgcg ccggtcgcgc
     3061 agcgggtcga ccacccgccc cacgtgctcg gtgcggcgca gctcacgggc caggcgagcc
     3121 acctcgcgcc tgacggcggg gctgcggacg tccagccgcc cgtggtcgcg ggctcggacc
     3181 agcagcacga tgcccggcgt ggcgtcgtag ccgagctggt cccgcagctg ctcggtggcc
     3241 cgctcgctct gggacgcgct gtcggtgaag ccggcggcct tgaggtggcg ctccacgtcg
     3301 tgaccgaaca c
//
LOCUS       DASUPW010000314         3689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_630754, whole genome shotgun sequence.
ACCESSION   DASUPW010000314 DASUPW010000000
VERSION     DASUPW010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630754"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1339
                     /gene="pdhA"
                     /locus_tag="VFY44_07050"
     CDS             134..1339
                     /gene="pdhA"
                     /locus_tag="VFY44_07050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX5910234.1"
                     /translation="MATEDATRGRGPEHRGVEAGEHKLPSRETAEKILAKMQLIRRFE
                     ERAGEMYAKAKIGGFLHLCIGEEATIVGATQALRDTDYLMSTYREHGQALARGTSPES
                     VMAELFGKADGCSGGRGGSMHLFDMERRFLGGYGIVGGNLPLAAGVALASDYTGTEDA
                     TLCMFGDGATNTGNFGETMNFAALWSLPVVFMVINNQFGMGTALERHSAVTDLSEKAA
                     GYGVPGSRCDGMDVLDTHAVVSAALEKARTDRKPQLVEAVTYRYRGHSMADPEEYRSK
                     DEVEEWRPRDPVVTFPARLVEEGLLGEDDVERMDKEAIAKVDAAVEFADNSPFPDLAS
                     LYDDIYVIGEQVRDPWYSTDTRSPDTHRGEQEREAGDVARSLAEAGAAHEDDTDDEMR
                     EARGRGEGG"
     gene            1339..2352
                     /locus_tag="VFY44_07055"
     CDS             1339..2352
                     /locus_tag="VFY44_07055"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX5910235.1"
                     /translation="MAATDTESAVQTMRYREALNLALREEMERDENVFLMGEDIGVFQ
                     GAFKVTAGLMEEFGEKRVRDTPISENSIVGLGVGAAMTGLRPVVELMTINFSLLALDQ
                     VINSAASIRYMFGAQVKVPLVIRMPQGAGHQLGPTHSHSFEAIYSHIPGLLVAVPTTA
                     ADARGLLKAAIRDDNPVIFIEHETLYGRKGEVDTSDDEPLAFGQAAIRREGADVTIVG
                     ISRMAETALKGAEKLASDHDIEAEVIDPRTLRPLDLDTILESVRKTNRAVIVEEGWPH
                     GGVGANMAALIQEQAFDHLDAPVARVAGADVPMPYSKPLEQASFPHEEHVVQAALSTF
                     RDL"
     gene            2352..3635
                     /locus_tag="VFY44_07060"
     CDS             2352..3635
                     /locus_tag="VFY44_07060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX5910236.1"
                     /translation="MATDVNMPRLSDSMEEGTVLKWLVEVGGEVKRGAPLVEIETDKA
                     NMTYEADTDGVLLEVVASEGDTLEVGEVIARIGAEGEEPAEAPATKEEPATAEQPEPE
                     PERAEEAPEPPAPAPAGNGGERIKASPVARRIAGELGVELASLEGSGPGGRIIKADVE
                     SAAENGAPAKTEPEPVPEPAAEETAPAPKAKPAEAGVKGEPEVIELTRLQRTVSRRMA
                     ESKATAPDFALEVEVDMTLCIELRSRLKETAGDGAVPSFNDMIVKAAALAAREHPKVN
                     GAYRDGRFELYPEVNVGVAVAAQDALVVPVVRGADQKSLGEIARNARALIEKVRDGSI
                     TPPELSGGTITVSNLGMYGIDRFTAIINPPQASILSVGALKKKPAVDEKGRVVARDLM
                     TITMACDHRILYGADAAQFLARIRELLEEPLRLAL"
BASE COUNT          598 a         1282 c         1356 g          453 t
ORIGIN      
        1 ggccaagcag taagcctgcg acgaaacggg catccatgcc cgtttcgtca acgtcgacgg
       61 accaactgcc cggcccccca tctgtcggga atcccgtttg cgcccggctc ccagtggcta
      121 gagtcgactg cagatggcca ccgaggacgc cacgcgcggc cggggccccg agcaccgggg
      181 cgttgaggcc ggcgagcaca agcttccgag ccgtgagacg gccgagaaga tcctggccaa
      241 gatgcagctc atccgccgct tcgaggagcg cgccggcgag atgtacgcca aggcgaagat
      301 cggcggcttc ctgcacctgt gcatcggcga ggaggccacg atcgtcggcg ccacccaggc
      361 gctgcgcgac accgactacc tgatgtcgac ctaccgcgag cacggccagg cgctggcccg
      421 cggcacctcg cccgagtcgg tcatggccga gctgttcggc aaggccgacg ggtgctcggg
      481 cgggcgcggc gggtcgatgc acctgttcga catggagcgc cgcttcctgg gcggctacgg
      541 catcgtcggc gggaacctgc cgctggccgc gggggtcgcg ctggccagcg actacaccgg
      601 caccgaggac gccacgctgt gcatgttcgg cgacggcgcc accaacaccg gcaacttcgg
      661 cgagaccatg aacttcgcgg cgctgtggag cctgccggtg gtcttcatgg tgatcaacaa
      721 ccagttcggg atgggcacgg cgctggagcg ccactccgcg gtgaccgacc tgtccgagaa
      781 ggccgcgggc tacggcgtgc ccggcagccg ctgcgacggc atggacgtgc tggacaccca
      841 cgcggtggtg tccgcggcgc tggagaaggc gcgcaccgac cgcaagccgc agctggtgga
      901 ggccgtgacc taccgctacc ggggccactc gatggcggac cccgaggagt accgcagcaa
      961 ggacgaggtc gaggagtggc gcccgcgcga cccggtggtc accttccccg cgcgcctggt
     1021 cgaggagggc ctcctgggcg aggacgacgt ggagcggatg gacaaggagg cgatcgcgaa
     1081 ggtcgacgcc gccgtggagt tcgccgacaa ctcgccgttc cccgacctgg cctcgctgta
     1141 cgacgacatc tacgtgatcg gcgaacaggt gcgcgacccc tggtactcga ccgacacgcg
     1201 ctcgcccgac acccaccgcg gcgagcagga gcgcgaggcc ggtgacgtgg cgcgctcgct
     1261 ggccgaggcc ggcgccgccc acgaggacga caccgacgac gagatgcgcg aggcgcgcgg
     1321 gcgcggcgag ggcggctgat ggcggccacc gacaccgagt ccgcggtcca gacgatgcgc
     1381 taccgcgagg cgctgaacct ggcgctgcgc gaggagatgg agcgcgacga gaacgtgttc
     1441 ctcatgggcg aggacatcgg cgtgttccag ggcgcgttca aggtcaccgc cggcctgatg
     1501 gaggagttcg gcgagaagcg cgtgcgcgac acgccgatct ccgagaactc gatcgtgggc
     1561 ctgggcgtgg gcgcggccat gaccgggctg cggccggtgg tcgagctgat gacgatcaac
     1621 ttctcgctgc tggcgctgga ccaggtgatc aacagcgccg cctcgatccg gtacatgttc
     1681 ggcgcccagg tcaaggtgcc gctggtcatc cggatgccac agggcgcggg ccaccagctg
     1741 ggcccgaccc actcgcattc gttcgaggcc atctactcgc acatcccggg cctgctggtg
     1801 gccgtgccca ccaccgccgc cgacgcccgc ggcctgctca aggcggcgat ccgcgacgac
     1861 aacccggtga tcttcatcga gcacgagacc ctgtacgggc gcaagggcga ggtggacacc
     1921 tccgacgacg agccgctggc cttcggccag gcagcgatcc gccgcgaggg cgccgacgtg
     1981 accatcgtcg gcatctcccg gatggccgag accgcgctca agggcgccga gaagctggcc
     2041 tccgaccacg acatcgaggc cgaggtgatc gacccgcgga cgctgcgccc gctggacctg
     2101 gacacgatcc tggagtccgt gcgcaagacc aaccgcgcgg tgatcgtcga ggagggctgg
     2161 ccccacggcg gcgtgggcgc gaacatggcg gccctgatcc aggagcaggc cttcgaccac
     2221 ctggacgcgc ccgtcgcacg cgtggcgggg gccgacgtgc cgatgcccta ctccaagcca
     2281 ctggagcagg cgtcgtttcc gcacgaggag cacgtggtcc aggccgccct gtccaccttc
     2341 cgcgacctct agtggccacc gacgtcaaca tgccgcggct gtccgactcg atggaggagg
     2401 gcacggtgct gaagtggctg gtcgaggtcg gcggagaggt caagcgcggc gcgcccctgg
     2461 tggagatcga gaccgacaag gccaacatga cctacgaggc cgacaccgac ggcgtgctgc
     2521 tggaggtcgt ggccagcgaa ggcgacacgc tcgaggtggg tgaggtcatc gcgcgcatcg
     2581 gcgcagaggg tgaggagcct gccgaggcgc cggcgacgaa ggaggagccc gccacggcgg
     2641 agcagcccga gccggaaccg gagcgcgccg aggaggcgcc tgagccgccg gcgccggcgc
     2701 cggccggcaa cggcggtgag cggatcaagg cctcccccgt ggcgcgccgc atcgccggcg
     2761 agctgggcgt cgagctcgcg tcgctggagg gctcgggtcc cggcgggcgc atcatcaagg
     2821 ccgacgtgga gtcggcggcg gagaacggcg cgcccgcgaa gaccgagccg gagcccgtcc
     2881 ctgaacccgc ggcggaggag acggccccgg ccccgaaggc caagcccgcc gaggccggcg
     2941 tcaagggcga gcccgaggtc atcgagctga cccgcctgca acgcaccgtc tcccgccgga
     3001 tggccgagag caaggccacc gcgcccgact tcgcgctcga ggtcgaggtg gacatgacct
     3061 tgtgcatcga gctgcgctcg cgcctgaagg agaccgcggg cgacggcgcg gtgccgtcgt
     3121 tcaacgacat gatcgtcaag gccgccgccc tggccgcgcg cgagcacccc aaggtcaacg
     3181 gcgcctaccg cgacggcagg ttcgagctct accccgaggt gaacgtgggc gtggccgtgg
     3241 ccgcccagga cgccctggtc gtgcccgtgg tgcggggcgc cgaccagaag tcgctgggcg
     3301 agatcgcgcg caacgcgcgg gcgctgatcg agaaggtgcg cgacgggtcg atcacgccgc
     3361 ccgagctgtc ggggggcacg atcaccgtct cgaacctggg catgtacggg atcgaccggt
     3421 tcacggccat catcaacccg ccgcaggcct cgatcctgtc cgtgggcgcg ctgaagaaga
     3481 agcccgccgt ggacgagaag gggcgcgtgg tggcgcgcga cctgatgacg atcaccatgg
     3541 cctgcgacca ccgcatcctc tacggcgccg acgccgcgca gttcctcgcc cggatccggg
     3601 agctgctcga agagccgctg cggctggccc tctgagccgg ccggccgccg ccctggcgct
     3661 gctctgcgcc gcccaggtcg tggacgtga
//
LOCUS       DASUPW010000315         5472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_907904, whole genome shotgun sequence.
ACCESSION   DASUPW010000315 DASUPW010000000
VERSION     DASUPW010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907904"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VFY44_07065"
     CDS             complement(<1..454)
                     /locus_tag="VFY44_07065"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEX5910237.1"
                     /translation="MLVAVAAAAAGGCGYESQGESGGKPLALTTFTVTADMVRNVAGN
                     RVEVASITKPGAEIHGYEPTPSDLKRGARADIVFDNGMGLERWFDQFIDEADAPRVTL
                     STGVRSIPIAGASDYEGKPNPHAWMSPANARVYVENIRKGLTRLDPAGA"
     gene            564..1562
                     /locus_tag="VFY44_07070"
     CDS             564..1562
                     /locus_tag="VFY44_07070"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX5910238.1"
                     /translation="MERIEHLRQRFSISAQTHRRLAFAALVLLVLIVFTGSAVRLTGS
                     GLGCPTWPKCTETSIHSELDTHGVIEFGNRVLTSLVSAGAIAAALGSFLRRPLHPLRR
                     DLAWIGLLLPLGVLVQAVIGGLSVLYKLAPGWVITHYLVSMVLLIASFALWWRSRLEP
                     DQIAEVRSERSVVWAVRALCVYAFVAISVGTWATAAGPHAGSAGTGEFVHRLDFWGRE
                     TLSNLINTHGTLAGILGVATVACWLLARARRGNAELLLTLKLTAGLMALQGIVGIVQY
                     RLELPSEIVWVHVALATLTWVGFVHVWFAAGRPVAGPSEEPASPSARRLVGEPVGS"
     gene            1566..1919
                     /locus_tag="VFY44_07075"
     CDS             1566..1919
                     /locus_tag="VFY44_07075"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX5910239.1"
                     /translation="MPAWEDVVRIGLGFPGVFESTSYGTPALKAKKQAGTMCRLRTDP
                     DALVVRVRDMGEREALLQNGGPAFFTVPHYDGYPYVLIDLDRVDPGELAELVEDAWRI
                     MAPKRAVTRYDDQRG"
     gene            1975..3666
                     /locus_tag="VFY44_07080"
     CDS             1975..3666
                     /locus_tag="VFY44_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910240.1"
                     /translation="MRLRPLALACALALLAVPASASAAVNVTGFSVTPSTTQAGGHPD
                     LKISTSFAASPASDDTKDLVVKFPAGLIGNPNAVPKCTTAQFGADACPGNTAVGSVAV
                     TAEATLALVINQTVTSPGTVYNLPPVGAEPARLGIKIRPAAVGPLTFQSISLVSVAKL
                     GPETGYALETTIPNQPRTVASSAGNVDLNIKQVDLSLRGQPGATAFLTNPSSCAPGTF
                     STTVVPYDSGAPSSRTAPYTATNCAALPFSPKASGTIGSKGRNKKGSLISLTTSFDFP
                     AGSGSLKDSVVILPKDVTSNLDALTRACDLVTPVESCPATSTIGTAEAATPLLAVPLR
                     GPVVLKKSAGGPYPSLVVSLRGAAPLTLEGKVDIAQNRLRNNFPGAPDIPLSKFKLSI
                     DGGKGGLLLAVDDLCRANRANAADVRLTGWNGKTVTSKPKLAPQGCKGYKPPRPRAAA
                     SIGRRSKTLKLRVSAGTYTRLRVVSASLPKGIAVSRKGLRGTAGKKLRGKSLRAKGRK
                     LKATVSRKGAASVRLTAKRVKVSRALVGRRVKIRVAAKDDTGRTVKLTVRARVRR"
     gene            complement(3663..4784)
                     /locus_tag="VFY44_07085"
     CDS             complement(3663..4784)
                     /locus_tag="VFY44_07085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5910241.1"
                     /translation="MPGTADVVVIGGGVMGTSILFHLAEAGVTDAVLVERGQLGGGSS
                     SKAAGGVRTQFSDELNVLLAKRSLAAFEDFGTRPGWEIDLKQVGYLFLLTREEDVRHF
                     EAGVALQNRLGVPSRMLTPAEAQGVCPPIDPGGMLAAAFCPTDGHLTPEAVVAGYAFA
                     ARGLGAHIETSCEVTGLRPGVVQTDHGDIAAPTVICAAGAWSAAVGEMAGVHLPVTPV
                     RRQIVFTEPIAGLPDELPMTIDFASSFYFHREGPGLLMGMSYAGQEPGFSTEQTDDWL
                     PGLQSALERVAPSVAGAGIKGGWGGLYEVSPDHNALIGRAGDLLYATGFSGHGFLQAP
                     AVGEVVRDLFLGREPFADVAPFSAERFHAGRERPERNVV"
     gene            4834..>5472
                     /locus_tag="VFY44_07090"
     CDS             4834..>5472
                     /locus_tag="VFY44_07090"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5910242.1"
                     /translation="MRTGGQALVETLEQLGVRLCFGLPGVHNLPAWEALRESPIRLVG
                     VRHEQTAVYAADGHARTTGELGVALTTTGPGAANALGATGEAWASHSPVLVIATDIPT
                     TLRRAGVYRGVLHETRNQGAMFAPVTKAVVRVGQDWTLEGAIERAAAVALTPPTGPVY
                     LEIPTDLLRGKARSPSLVAVGERPQPPLPDLDVAGALCAAAKRPLLWVGGGAK"
BASE COUNT          740 a         2036 c         1936 g          760 t
ORIGIN      
        1 cggctccggc ggggtccagc cgggtcaggc ccttgcggat gttctccacg tacacgcgcg
       61 cgttcgcggg cgacatccag gcgtgcgggt tgggcttgcc ctcgtagtcg ctggcgccgg
      121 cgatagggat cgacctgacc cccgtgctca gcgtgacccg gggcgcgtcg gcctcgtcga
      181 tgaactgatc gaaccagcgc tccagcccca tgccgttgtc gaacacgatg tccgcgcgcg
      241 cgccgcgctt gaggtcgctc ggcgtgggct cgtagccgtg gatctcggcg ccgggcttgg
      301 tgatggacgc cacctcgacc cggttgcccg cgacgttgcg gaccatgtcg gccgtgaccg
      361 tgaaggtggt cagggccagc ggcttgcccc cggactcgcc ctgggactcg tagccgcagc
      421 cgcccgcggc ggcggccgcg acggcaacca gcacggctgt ggcgattctc ttcacagcac
      481 taccgtaaca ggaagttagt caagactaac tttgttcttg caccatggcg caaagtcctg
      541 cgcttgtacc ctgtgcggcc cccatggagc gcatcgagca cctccgccag cgcttctcca
      601 tcagcgcgca gacgcaccgc cgcctggcct tcgccgcgct ggtgctgctg gtgctgatcg
      661 tgttcaccgg ctccgccgtg cgcctgacgg gatcgggcct gggctgcccc acctggccca
      721 agtgcaccga gacctcgatc cacagcgagc tggacacgca cggggtgatc gagttcggca
      781 accgcgtgct gacctcgctg gtgtcggccg gggcgatcgc cgccgcgctg ggcagcttcc
      841 tgcgccggcc gctgcacccg ctccggcggg acctggcgtg gatcggcctc ctgttgcccc
      901 tcggggtcct ggtgcaggcc gtgatcggcg gcctgtcggt gctctacaag ctggcgccgg
      961 gctgggtgat cacccactac ctggtgtcga tggtgctgct gatcgcctcc ttcgcgctgt
     1021 ggtggcgctc gcggctggag cccgaccaga tcgccgaggt gcgctccgag cgctcggtgg
     1081 tgtgggccgt gcgcgcgctg tgcgtctacg cgttcgtggc catcagcgtc gggacgtggg
     1141 ccacggcagc cgggccccac gcgggctcgg cgggcaccgg cgagttcgtg caccggctgg
     1201 acttctgggg tcgcgaaacg ctctcgaacc tgatcaatac ccacggcacg ctggccggga
     1261 tcctgggcgt ggccacggtg gcctgctggc tgctggcgcg ggcgcgcagg ggcaacgccg
     1321 agctgctgct gacgctgaag ctgaccgcgg gcctgatggc gctccagggc atcgtgggca
     1381 tcgtccagta ccggctcgag ctgccctcgg agatcgtgtg ggtccacgtg gcgctggcca
     1441 cgctgacgtg ggtcggcttc gtacacgtgt ggttcgccgc cgggcgcccg gtggcgggcc
     1501 cgtccgagga gccggccagc cccagcgccc gtcgcctcgt gggcgagccg gtgggcagct
     1561 gaccgatgcc ggcgtgggag gacgtggtcc ggatcgggct cggcttcccc ggcgtgttcg
     1621 agtccacgtc ctacggcacg ccggcgctga aggccaagaa acaggccggg acgatgtgcc
     1681 ggctgcgcac cgacccggac gccctggtcg tgcgcgtgcg cgacatgggc gagcgcgagg
     1741 cgctgctcca gaacggcggc cccgccttct tcaccgtgcc ccactacgac ggatacccgt
     1801 acgtgctgat cgacctggac cgggtcgacc ccggcgagct ggccgagctg gtcgaggacg
     1861 cgtggcggat catggcgccc aagcgcgccg taacccggta cgacgaccag cgaggttgag
     1921 ttgacgttag gtgcccttgc ggcgcgaggg tgccgcgcag gacactgccc ggtcgtgaga
     1981 ctccgacccc tggcgctcgc gtgcgccctc gccctgctgg ccgtccccgc gtccgcctcg
     2041 gccgccgtca acgtgaccgg cttcagcgtc acccccagca ccacccaggc cggcgggcat
     2101 cccgacctga agatctcgac gtcgttcgcg gccagcccgg ccagcgacga caccaaggac
     2161 ctggtcgtga agttcccggc cggcctgatc ggcaacccga acgccgtgcc caagtgcacg
     2221 accgcccagt tcggcgccga cgcctgcccg ggcaacaccg cggtcggctc ggtggcggtc
     2281 acggcggagg ccacgctggc cctcgtgatc aaccagaccg tgacgtcccc cggcaccgtc
     2341 tacaaccttc cgcccgtggg cgcggagccg gcgcggctgg gcatcaagat ccgtcccgcc
     2401 gccgtggggc cgctgacctt ccagtcgatc tcgctggtca gcgtggccaa gctcggcccc
     2461 gagacgggct acgcgctcga gaccacgatc cccaaccagc cccgcaccgt tgccagctcg
     2521 gcgggcaacg tggacctcaa catcaagcag gtcgacctgt cgctgcgcgg ccagcccggc
     2581 gcgacggcgt tcctgaccaa cccgagctcg tgcgcccccg gcaccttctc gaccacggtc
     2641 gttccctacg acagcggcgc gccgtccagc cgcaccgccc cgtacacggc caccaactgc
     2701 gccgcgctgc cgttctcgcc caaggcctcg gggacgatcg gctccaaggg gcgcaacaag
     2761 aaggggtcgc tgatctcgct gaccacgtcg ttcgacttcc cggcgggcag cggctcgctc
     2821 aaggactcgg tggtcatcct gcccaaggac gtgacctcga acctggacgc gctcacgcgc
     2881 gcatgtgacc tggtcacgcc cgtcgagtcc tgccccgcca ccagcacgat cggcaccgcc
     2941 gaagccgcga cgccgctgct ggccgtgccg ctgcgcggcc cggtggtgct caagaagtcc
     3001 gccggcggcc cgtacccgtc gctggtggtc agcctcaggg gtgccgcccc gctgacgctc
     3061 gagggcaagg tggacatcgc ccagaaccgc ctgcgcaaca acttcccggg cgcccccgac
     3121 atcccgctgt ccaagttcaa gctgtcgatc gacggcggca agggcggcct gctgctggcg
     3181 gtcgacgacc tgtgccgcgc caaccgcgcg aacgcggccg acgtccggct gaccggctgg
     3241 aacggcaaga ccgtgaccag caagccgaag ctggcgcccc agggctgcaa gggctacaag
     3301 ccgcccaggc cgcgtgcggc cgcgtcgatc gggcggcgca gcaagacgct gaagctgcgc
     3361 gtctcggccg gcacctacac ccgcctgcgc gtggtctcgg ccagcctgcc caagggcatc
     3421 gcggtcagcc gcaagggcct gcgcgggacc gccggcaaga agctgcgcgg caagtcgctg
     3481 cgggccaagg gccgcaagct caaggccacg gtctcgcgca agggcgcggc gtcggtccgc
     3541 ctgacggcga agcgggtgaa ggtcagccgg gccctggtgg gcaggcgcgt gaagatccgc
     3601 gtggccgcca aggacgacac cggccgcacg gtcaagctga ccgtgcgcgc ccgcgtccgc
     3661 cgctagacca cgttgcgctc ggggcgctcg cggccggcgt ggaagcgctc ggccgagaac
     3721 ggcgcgacgt cggcgaacgg ctcccgcccc aggaacaggt cgcgcaccac ctcgccgacc
     3781 gccggggcct gcaggaaccc gtggcccgag aagcccgtgg cgtacagcag gtcgcccgcg
     3841 cgcccgatca gcgcgttgtg gtcggggctg acctcgtaca ggccgcccca gccccccttg
     3901 atcccggcgc cggccaccga tggcgccacc cgctcgaggg cgctctgcag gccgggtagc
     3961 cagtcgtcgg tctgctcggt cgagaagccg ggctcctgcc cggcgtagga catgcccatc
     4021 aacagccccg gcccctcgcg gtggaagtag aagctgctcg cgaagtcgat cgtcatcggc
     4081 agctcgtccg gtaggcccgc gatcggctcg gtgaacacga tctggcgccg tacgggcgtg
     4141 accggcaggt ggacgccggc catctccccc acggcggcgg accaggcacc cgcggcgcag
     4201 atcacggtgg gcgccgcgat gtcgccgtgg tcggtctgca ccacgcccgg ccgcaggccg
     4261 gtgacctcgc agctcgtctc gatgtgcgcc ccgaggccgc gggcggcgaa cgcgtagccg
     4321 gccaccaccg cctcgggcgt caggtggccg tccgtcgggc agaacgccgc cgccagcatg
     4381 ccgccgggat cgatcggggg gcagaccccc tgcgcctcgg ccggcgtgag catccggctg
     4441 ggcacgccga gccggttctg cagcgccacg ccggcctcga agtggcgcac gtcctcctcg
     4501 cgcgtgagca ggaacaggta gcccacctgc ttgaggtcga tctcccaccc gggacgggtg
     4561 ccgaagtcct cgaacgcggc caggctgcgc ttggccagca gcacgttcag ctcgtccgag
     4621 aactgcgtgc gcacgccgcc ggccgccttg ctggacgagc cgccgcccag ctggccgcgc
     4681 tccaccagaa ccgcatcggt cacgccggcc tcggccaggt ggaacaggat gctcgtgccc
     4741 atcacgccgc cgccgataac cacgacgtcg gcggtgcctg gcaacggtgc tcccctcatg
     4801 gccgctgacc ctgccagatg cgaggctgcc cgtgtgcgaa cgggtggaca ggcgctggtc
     4861 gagacgctgg agcagctcgg cgtgcggctg tgcttcggac tccccggcgt gcacaacctc
     4921 ccggcgtggg aggcgctgcg cgagtcgccg atccgcctgg tcggcgtgcg ccacgagcag
     4981 accgccgtgt acgccgcgga tggccacgcg cgcacgaccg gcgagctggg cgtcgcgctg
     5041 accaccaccg gcccgggcgc cgccaacgcg ctgggcgcga ccggcgaggc gtgggcctcg
     5101 cactcgccgg tgctggtgat cgccaccgac atccccacca cgttgcgccg cgccggcgtc
     5161 taccgcggcg tgctgcacga gacgcgcaac cagggtgcga tgttcgcgcc ggtgaccaag
     5221 gcggtggttc gcgtgggtca ggactggacg ctcgagggcg caatcgagcg cgcggcggcg
     5281 gtggcgctca cgcctccgac gggccccgtc tacctggaga tccccaccga cctgctccgc
     5341 ggcaaggccc gctcgcccag cctggtcgcg gtcggggaac gcccgcagcc gccgctcccg
     5401 gacctggatg tggcgggcgc gctgtgcgcc gcggccaagc ggccgttgct gtgggtcggc
     5461 ggcggcgcga ag
//
LOCUS       DASUPW010000316         2814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1599892, whole genome shotgun sequence.
ACCESSION   DASUPW010000316 DASUPW010000000
VERSION     DASUPW010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599892"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VFY44_07095"
     CDS             complement(<1..650)
                     /locus_tag="VFY44_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910243.1"
                     /translation="MLVLAGAAMAPASASAGSNQMTVIEDGAVFLGLTERDPDAALRE
                     AKALGADAVRMYISWRRAVPVIANQTVPPADFIPADPNNGWYDWRPYDNLVQRARAHG
                     LKVIISIGPAMPYWASREPSRCPHPVGNKLKLGLSCYWKPNPVAFGLFVGAVANRFKG
                     QASYYSLWNEPNLENYLLPQRQKTKAGVVDMGGKQLRELYIAGYRAIRANDRARSR"
     gene            complement(745..2253)
                     /locus_tag="VFY44_07100"
     CDS             complement(745..2253)
                     /locus_tag="VFY44_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910244.1"
                     /translation="MTRTLRTLLLTVAALAAFALVPAGASAGSKQFSIFEDDAVLLGL
                     TDKDPEKAMAEVKYLGGDVVRVFVVWSKVSPAKTSRTVPAGFDPADHRSPGYDWSLYD
                     AFIQRAQRNGLKVMLTLSGEIPFWASREPDRCPHFIGGYSNLALSCMWKPDQRLFEQF
                     SQAVATRYKGLVEYYSLWNEPNLEHYLYPQWSRAGRSTIDLGGKMLRELWTSGYRGLT
                     RVDPSVKNKVLFGETAAISSPMDTLYAALCLNQSGRPYKKGRVRRLMGCDKPAKLPIG
                     GLAVHPYNKDAVGTVFTRSFTKDSMSMAYTGRATRVLRRAERYGRIPRGRGVYITEFG
                     FQTKPPDKKGLSFNAQAKAMNESDRLFFGDKRIRAVAQFELYDVPEGVDDDVYNTGLR
                     TQACQLKPSWGAYRMPLVVTRLADDSVEVWGQVRPSNARTQVALFAGRKGGQLVRIGS
                     PRTNASGYFKVRVRRGQAAKLRYRFTWPAPNGETFASRTASAGKKIAYREKP"
     gene            complement(2336..>2814)
                     /locus_tag="VFY44_07105"
     CDS             complement(2336..>2814)
                     /locus_tag="VFY44_07105"
                     /inference="COORDINATES: protein motif:HMM:NF017555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HEX5910245.1"
                     /translation="DATRWFLLQRSHETAIDLDLELARRQSQDNPVYYAQYAHARIAS
                     ILRRAGDDRVGSALAADVAASAERLHPSARALVKRLATFPGEVVHAADRRAPHRMTTY
                     VTELAQDFSAFYRDCKVVGAAEEGGDEDLRIALSEATRRTIERSLGLLGVSAPSEM"
BASE COUNT          389 a          953 c         1029 g          443 t
ORIGIN      
        1 tgccgggagc gcgcgcggtc gttggcccgg atggcgcggt agcccgcgat gtacagctcg
       61 cgcagctgct tgccccccat gtccaccacg cccgccttgg tcttctggcg ctggggcagc
      121 aggtagttct ccaggttcgg ctcgttccag agcgagtagt acgaggcctg ccccttgaag
      181 cggttggcca ccgcgccgac gaacaggccg aaggcgaccg ggttcggctt ccagtaacac
      241 gacagcccca gcttgagctt gttgccgacc gggtgggggc agcgcgacgg ctcacgggag
      301 gcccagtagg gcatcgcggg cccgatgctg atgatgacct tgagcccgtg cgcacgggcg
      361 cgctggacca ggttgtcgta ggggcgccag tcgtaccagc cgttgttggg gtcggccggg
      421 atgaagtcgg ccggcggcac ggtctggttg gcgatgacgg gcaccgcccg gcgccacgag
      481 atgtacatcc ggaccgcgtc ggcccccagc gccttggcct cgcgcagcgc ggcgtccggg
      541 tcgcgctcgg tcagacccag gaacacggcg ccgtcctcga tcaccgtcat ctggttgctt
      601 ccggccgatg cggacgcggg cgccatggcg gccccggcca gaacgagcat ccctgcgagc
      661 gtcagtcggc gtatccgtgc cattgcgagt cgtcgaagct agctgagcat tcgcggaatc
      721 cccggcgccc gggttgcggc ggggttaagg cttctcgcgg taggcgatct tcttgcccgc
      781 ggaggcggtg cggctggcga aggtctcgcc gttgggtgcc ggccacgtga agcggtagcg
      841 cagcttggcc gcctggccgc ggcgcacgcg caccttgaag taaccggacg cgttggtgcg
      901 gggcgatccg atccgcacca gctggccgcc cttgcggccc gcgaacaggg ccacctgggt
      961 gcgcgcgttg gacggccgga cctggcccca gacctcgacc gagtcgtcgg ccaggcgggt
     1021 gaccaccagc ggcatccggt aggcgcccca ggagggcttg agctggcacg cctgcgtgcg
     1081 caggccggtg ttgtagacgt cgtcgtcgac cccttcgggc acgtcgtaga gctcgaactg
     1141 cgccacggcg cggatgcgct tgtcgccgaa gaacaggcgg tcggactcgt tcatggcctt
     1201 cgcctgggcg ttgaagctca gccccttctt gtccggcggc ttggtctgga agccgaactc
     1261 ggtgatgtac acgccgcgcc cgcgcgggat ccgcccgtag cgctcggccc ggcgcagcac
     1321 gcgggtggcg cggccggtgt aggccatgga catcgagtcc ttggtgaacg agcgggtgaa
     1381 taccgtgccc acggcgtcct tgttgtacgg gtgcacggcc aggccgccga tgggcagctt
     1441 ggctggtttg tcgcacccca tgagccgccg cacgcggccc ttcttgtagg gcctaccgct
     1501 ctggttcagg cacagcgcgg cgtagagcgt gtccatcggg gagctgatgg ccgccgtctc
     1561 gccgaacagg accttgttct tgaccgacgg gtccacccgg gtcaggccgc ggtagcccga
     1621 cgtccacagc tcgcgcagca tcttgccgcc caggtcgatc gtgctccgcc ccgcgcgcga
     1681 ccactgcggg tagaggtagt gctccaggtt cggctcgttc cagagcgagt agtactcgac
     1741 cagccccttg tagcgggtgg ccacggcctg ggagaactgc tcgaacagcc tctggtcggg
     1801 cttccacatg cacgagagcg ccaggttgga gtagccgccg atgaagtgcg ggcagcggtc
     1861 gggctcgcgc gaggcccaga acgggatctc acccgacagc gtgagcatga ccttcagccc
     1921 gttgcgctgc gcgcgctgga tgaaggcgtc gtacaggctc cagtcgtagc cgggcgacct
     1981 gtggtcggcc gggtcgaagc cggccgggac cgtccggctg gtcttcgccg gcgagacctt
     2041 cgaccacacg acgaacaccc gcaccacgtc gccgcccagg tacttgacct cggccatggc
     2101 cttctcgggg tccttgtcgg tcaggcccag cagcaccgcg tcgtcctcga agatcgagaa
     2161 ctgcttcgag ccggcgctgg cgccggccgg gacgagcgcg aacgcggcca gcgcggccac
     2221 ggtgagcagc agtgtgcgga gcgttcgggt cacttggaga aaggcaaccc gcggcacgcc
     2281 cccgagttgc gagccgcagg gtagtgactc ttgcggccgt ccatggccgc aggccctaca
     2341 tctcggacgg agcggacact cccagcaggc ccagcgagcg ctcgatggtg cggcgggtgg
     2401 cctcgctgag cgcgatccgc aggtcctcgt cgccgccctc ctcggccgcg ccgaccacct
     2461 tgcagtcgcg gtagaaggcc gagaagtcct gggccagctc ggtcacgtag gtggtcatcc
     2521 ggtgcggggc gcgccggtcc gcggcgtgga cgacctcgcc cgggaaggtg gccaggcgct
     2581 tgaccagcgc ccgcgcggag gggtgcagcc gctcggcgct ggcggccacg tcggcggcca
     2641 gcgcggagcc gacccggtcg tcgcccgccc tgcgcaggat gctggcgatg cgggcgtgcg
     2701 cgtactgggc gtagtacacg gggttgtcct ggctctgacg ccgggccagc tccaggtcca
     2761 ggtcgatggc cgtctcgtgg ctgcgctgga gcaggaacca gcgcgtggcg tcca
//
LOCUS       DASUPW010000317         2197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1322991, whole genome shotgun sequence.
ACCESSION   DASUPW010000317 DASUPW010000000
VERSION     DASUPW010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322991"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2197)
                     /locus_tag="VFY44_07110"
     CDS             complement(<1..>2197)
                     /locus_tag="VFY44_07110"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEX5910246.1"
                     /translation="HPVIEDEALDRIRAVESGPLIAQTLDFTWAIDQGEEGLEAALER
                     LFEEADKAIADGVGVLILSDRAVSHDRAAVPSLLALSAVHHHLVRSGTRTRVGLVVES
                     GEPRDPHHIAALIGYGAAAVNPYLMLECAEGAGEKVIASMSKSLLKIISKMGISTIRS
                     YNGAQVFEVVGLDRGLVDRHFTGTPSRVGGIDLEGLAREALHRHSRAWPDAHGEALPD
                     HVEDSLLPAASQELLPQGGQYQWRRDGEKHMWEPGTITGLQSVARGGGRETYKDFARR
                     VDEENASFNLLRGLMRLRTDREAISVSEVEPVAEIVKRFTTGAMSLGSLSSEAHETLA
                     IAMNRLGGRSNTGEGGEDPRRFAPDPNGDLRRSAIKQVASGRFGVTADYLVNADQIQI
                     KVAQGAKPGEGGQLPGHKITAEFGRLRLSTPGVELISPPPHHDIYSIEDLKQLIYDLR
                     CVNPRASISVKLCAEVGVGTVAAGVVKANADHIVIAGHDGGTGAASLSSIHHAGVPWE
                     IGLAETQQTLIRNDLRSRVMLQADGQMRTGRDVVVAALLGADEVGFSTAPLVATGCVM
                     MRVCHLNTCPVGIATQDPVLRDRFKGKPEHVVRYLLAVAEEVRELMAGLGFATFQEMV
                     GQVEMLEQDPDIDRWKAGGIDLSDLLSVPEDTEGLPRRHEDPPTPVLDDSVDHIQLLP
                     AAARAIRDDEPVRLYREIANVHRTVGALLSGEIVRRRGPEGLPEGTIDVV"
BASE COUNT          281 a          793 c          804 g          319 t
ORIGIN      
        1 accacgtcga tcgtgccctc cggcaggccc tcggggcccc ggcggcggac gatctcgccg
       61 gacagcagcg cgccgacggt gcggtgcacg ttggcgatct cgcggtacag gcgcaccggc
      121 tcgtcgtcgc ggatggcgcg cgccgcggcg gggaggagct ggatgtggtc gaccgagtcg
      181 tccagcaccg gggtcggcgg gtcctcgtgg cggcgcggca ggccctcggt gtcctcgggc
      241 acgctcagca ggtcggacag gtcgatgccg ccggccttcc agcggtcgat gtcggggtcc
      301 tgctccagca tctccacctg gccgaccatc tcctggaagg tggcgaagcc cagcccggcc
      361 atcagctcgc ggacctcctc ggccaccgcc agcaggtagc gcaccacgtg ctcgggcttg
      421 cccttgaagc ggtcgcgcag cacggggtcc tgggtggcga tcccgaccgg gcaggtgttc
      481 aggtggcaga cccgcatcat cacgcagccc gtggccacca gcggggcggt cgagaagccc
      541 acctcgtcgg cgcccagcag cgcggccacg accacgtcgc ggccggtgcg catctggccg
      601 tcggcctgga gcatcacgcg cgagcgcagg tcgttgcgga tcagcgtctg ctgcgtctcg
      661 gccaggccga tctcccacgg cacgcccgcg tggtggatcg aggacagcga ggcggccccg
      721 gtgccgccgt cgtggccggc gatcacgatg tggtcggcgt tggccttgac cacgcccgcg
      781 gccaccgtgc ccacgccgac ctcggcgcac agcttcaccg agatcgacgc gcgcgggttc
      841 acgcagcgca ggtcgtagat cagctgcttg aggtcctcga tcgagtagat gtcgtggtgc
      901 ggcggcggcg agatcagctc gacgcccggg gtcgacagcc gcaggcggcc gaactcggcg
      961 gtgatcttgt ggccgggcag ctggccgccc tcgccgggct tggcgccctg ggcgaccttg
     1021 atctggatct ggtcggcgtt gaccaggtag tcggcggtca cgccgaagcg gccggacgcc
     1081 acctgcttga tcgccgagcg gcgcaggtcg ccgttggggt cgggggcgaa ccggcgcggg
     1141 tcctcgccgc cctcgccggt gttcgaccgc ccgcccaggc ggttcatggc gatggccagc
     1201 gtctcgtgcg cctccgagga cagcgagccc aggctcatgg cgccggtggt gaagcgcttg
     1261 acgatctcgg ccaccggctc gacctcggac accgagatgg cctcgcggtc ggtgcgcagg
     1321 cgcatcagcc cgcgcagcag gttgaacgag gcgttctcct cgtccacccg gcgggcgaag
     1381 tccttgtagg tctcgcggcc gccgccacgg gccacggact gcaggccggt gatcgtcccc
     1441 ggctcccaca tgtgcttctc gccgtcgcgg cgccactggt actggccgcc ctgaggcagg
     1501 agctcctggg aggcggccgg cagcaacgag tcctcgacgt ggtcgggcag cgcctcgccg
     1561 tgggcgtcgg gccacgcgcg cgagtggcgg tgcagcgcct cccgggccag gccctcgagg
     1621 tcgatcccgc ccacccgcga cggcgtgccg gtgaagtggc ggtcgaccag cccgcggtcc
     1681 agcccgacga cctcgaacac ctgcgccccg ttgtaggagc ggatggtcga gatgcccatc
     1741 ttggagatga tcttcagcag cgacttgctc atcgaggcga tgaccttctc gccggcgccc
     1801 tcggcgcact cgagcatcag gtacgggttc accgccgccg cgccgtagcc gatcagggcc
     1861 gcgatgtggt gcgggtcacg cggctcgccc gactccacga ccaggcccac gcgcgtgcgc
     1921 gtgcccgagc gcacgaggtg gtggtgcacg gcggacagcg ccagcagcga cggcaccgcg
     1981 gcgcggtcgt ggctgacggc gcggtcgctc aggatcagga cgccgacgcc gtcggcgatg
     2041 gccttgtcgg cctcctcgaa caggcgctcg agcgcggcct ccaggccctc ctcgccctgg
     2101 tcgatcgccc aggtgaagtc cagtgtctgc gcgatcagcg ggccgctctc gaccgcgcgg
     2161 atgcggtcca gcgcctcgtc ctcgatcacc gggtgct
//
LOCUS       DASUPW010000318         6755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_355411, whole genome shotgun sequence.
ACCESSION   DASUPW010000318 DASUPW010000000
VERSION     DASUPW010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6755
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355411"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="VFY44_07115"
     CDS             complement(<1..728)
                     /locus_tag="VFY44_07115"
                     /inference="COORDINATES: protein motif:HMM:NF016129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HtaA domain-containing protein"
                     /protein_id="HEX5910247.1"
                     /translation="MPSSRFLAAALALSIPLLAAPAATGAVVTGGNVDWGVKQSFRSY
                     VTGPIAAGSITASEGASTNPGGTFRFPITGGDQIETQRVSVGARGKVKFSGHLGALDF
                     TIFNVRVVLDGTASGPCLVADADTKKLNGGGVTESYRDVRFAKLNTTGLTPSTAGGTT
                     TWSGVPATLTAEGSPAFAGFYDPGEELDPVTFSYATGSGTGSAAGACEKRGSVVTVPP
                     GGTPPASKPAGATLKLGKPSKGQL"
     gene            complement(774..2039)
                     /locus_tag="VFY44_07120"
     CDS             complement(774..2039)
                     /locus_tag="VFY44_07120"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5910248.1"
                     /translation="MLSDTFTSESLRAAAVSALPGAAVFAVDRDMRLLLADGPALRRH
                     GYDPATLAGRRLADVAPAHSYAELEPHYRAALAGEPRTFEHSSSDATGWYLTHIAPLI
                     EGEQIVGAVAVSQDITSRRLADDELRAVTTLFETAFAAAPIGMALVGVDGSFQRCNQA
                     LCDLLGYEEDELMALSFQDITHPEDLDSDMAHVQRLLAGEASSYTMEKRYVTRTGRLV
                     WVLLAVSLVRDDDGAPSHFISQIKDITETRRMEERLRQLADHDAVTDLLNRRRFEDEL
                     VRQVGRCKRYGETACLLILDVDDFKGVNDAFGHRTGDAALQQVALILNGRLRDTDVVA
                     RIGGDEFAALLPGVTAQDARGLAEEVRTAIEELRVGSGGRHASVTASIGLKTLDRGTI
                     DEDSAFVAADRAMYESKAAGRNRVTVALP"
     gene            complement(2112..3194)
                     /locus_tag="VFY44_07125"
     CDS             complement(2112..3194)
                     /locus_tag="VFY44_07125"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5910249.1"
                     /translation="MVGAVLAGDGLERVAEIAGQHAGAPVAVVVPRLGTPSEEWAPYE
                     RYVAKRLAGGKPDRPPEVTAEVPIVSGGQELGAVLLLGAGGSDAGEYLHVAAVAALTE
                     VAVAEARDETEQNLRGSFLEELLTREDLDPGDIVRRARRLGCDLSEGAVGLCADPGER
                     APGRLLAAISSEMPGALAQVIPTPGGTGRVYALLPGPVDVARQVANRLGRQALIGLSS
                     HYSDPADVRRALEEAELVLGVTAAGGGPPGDEIGGGTYRLLFRVLASHPEEVRSFYED
                     TIAPLARYDEQYSTDLVGTLEAYLGQNCNMNATAAAIHAHRHTVSYRLERVKELSGLD
                     PFTSEDRERLGLGLKAYRIIEPKLHR"
     gene            complement(3328..3702)
                     /locus_tag="VFY44_07130"
     CDS             complement(3328..3702)
                     /locus_tag="VFY44_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX5910250.1"
                     /translation="MKKVEAFVRHEAFEPIRMELLDKGFPSLSIAEYKGSGRQKGITE
                     RYRGSELTIHLRPKLKLEIVVDDTDVGVITETILKHARTGQVGDGKIFVIPVEAAIRI
                     RTGEDGGEVLQAHETEEIPAGA"
     gene            complement(3699..5216)
                     /locus_tag="VFY44_07135"
     CDS             complement(3699..5216)
                     /locus_tag="VFY44_07135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX5910251.1"
                     /translation="MLILPATSLASSAELKNAVDGVDQGTLPIDTMWVVIAAILVLFM
                     QAGFMFLEVGFVRAKNAGTIVAKILTNLSIAAICYWAVGFAFAFGSGDVFGDNSTLAS
                     IIGSNGFFLQVAGTGGVDDLAGNSGIPVMSVSAASIDAKFLFQFAFCAVSLAIVWGST
                     LERIKYGAYVLYAIVFSAIIYPIGSHWVFGGDWLQNGDTPLMPTGMQDFAGSTAVHLI
                     GATGALAALLLLGPRKGKYGSDGKPRVIPGHSMPLVGLGVLILFVGWFGFNAGSTLGT
                     ADGRLADVAVITMLGAAGGVLGAFTVSMLRLKTIDIGMVGNGMIAGLVGITAPSGYVE
                     VWAAPIIGAVAGMIVVFGVILIDKKIDDPIGATSAHGLAGIWGTIACGFFTSPRLAQY
                     NAFGNPDGGLLYGGGPDQLIAQVVGVLIAFTFVFALSYGTFFLIKKTIGLRVTEEEED
                     AGLDIVEHGMYGYPEQFIPLPETGAASTGPAAPHAGEAPVAHQGRGDAVPGGVTA"
     gene            complement(5458..5796)
                     /locus_tag="VFY44_07140"
     CDS             complement(5458..5796)
                     /locus_tag="VFY44_07140"
                     /inference="COORDINATES: protein motif:HMM:NF021440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2149 domain-containing protein"
                     /protein_id="HEX5910252.1"
                     /translation="MSASRVSPRARTREDRGGDPLDGLVNLFDLGIVLAVAFLLAALS
                     SVKLSPSVLRENKAQARQQQSRDQVVQRKGERAGPIRLREGDRVVGKGRRLGTVYRLE
                     DGRTIIVRPR"
     gene            complement(5793..6452)
                     /locus_tag="VFY44_07145"
     CDS             complement(5793..6452)
                     /locus_tag="VFY44_07145"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX5910253.1"
                     /translation="MLPLAAVNGVERVIFDVAEALRIPVLVLALASIAFVLVEAGALA
                     AELVRRRGRGVLRLEEGITAIRARLATGDRAGAARTARGLGYNERMGDALAAIVEQRD
                     ADRAADRIAKRMAEYDYWSLKRLERTRILVRTGPAVGLMGTLIPLSPALEGLAQGNVE
                     QLSNDLRVAFSVTVAGLVVGVIAFGVSLVRDRLYAQDFSDVEYVLASLEGAPAPEPVP
                     A"
     gene            complement(6431..>6755)
                     /locus_tag="VFY44_07150"
     CDS             complement(6431..>6755)
                     /locus_tag="VFY44_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910254.1"
                     /translation="SGPARAPAAGPAATPRRSRPAPQPGGRQVEGTLLADATVVPPRP
                     AARKRAARTGNDRGDEPGPGIPTAAWGLLVTLGLAGTGAVIELRTVLPWPRRALGARC
                     CPWPR"
BASE COUNT          974 a         2434 c         2394 g          953 t
ORIGIN      
        1 ctcagctggc ccttgctggg cttcccgagc ttgagcgtcg cgccggccgg cttggaggcc
       61 ggcggcgtgc cgccgggcgg aaccgtgacc acgctgccgc gcttctcaca cgcccccgcg
      121 gccgacccgg tgccggatcc cgtcgcgtag ctgaacgtga ccgggtcgag ctcctcgccc
      181 gggtcgtaga agcccgcgaa ggcgggcgag ccctcggccg tcagcgtggc ggggacgccg
      241 gaccaggtgg tggtgccacc ggcggtggac ggcgtgaggc cggtggtgtt cagcttggcg
      301 aagcgcacgt cccggtagga ctcggtcacg ccgccgccgt tcagcttctt ggtgtccgca
      361 tcggccacca ggcacggccc cgaggccgtg ccgtccagca ccacgcgcac gttgaagatc
      421 gtgaagtcca gcgccccgag gtgaccggag aacttgacct tgccgcgggc gcccacgctg
      481 acccgctgcg tctcgatttg gtcgccgccg gtgatcggga agcggaacgt gccgccgggg
      541 ttggtggagg ctccctcgga ggcggtgatg gaaccagccg cgatcggccc cgtcacgtag
      601 ctgcggaagc tctgcttgac cccccagtcc acgttgccac cggtgaccac ggcccctgtc
      661 gccgccggcg ccgccaggag cgggatcgag agggccaggg cggcggcgag gaagcgggac
      721 gagggcatgc cgccatcata cttagggttc cctaatttcg cgtggccgcc cggctacggc
      781 agggctaccg tcacgcggtt gcgcccggcg gccttcgact cgtacatcgc ccggtcggcg
      841 gccacgaacg cggagtcctc gtcgatggtg ccccggtcca gcgtcttcag gccgatgctg
      901 gcggtcacgc tggcgtgccg tccaccggag ccgacccgga gctcctcgat cgcggtccgc
      961 acctcctcgg ccagcccgcg cgcgtcctgg gcggtcacgc cgggcagcag cgccgcgaac
     1021 tcgtcgccgc cgatcctggc caccacgtcg gtgtcacgca gccggccgtt caggatcagg
     1081 gccacctgct gcagcgcggc gtccccggtc cggtggccga aggcgtcgtt cacgcccttg
     1141 aaatcgtcca cgtcaaggat cagcagacag gcggtctcgc cgtagcgctt gcagcgcccc
     1201 acctggcgca ccagctcgtc ctcgaaccgg cgccggttca gcaggtcggt caccgcgtcg
     1261 tggtccgcca gctggcgcag ccgctcctcc atccggcgcg tctcggtgat gtccttgatc
     1321 tgggagatga agtgcgacgg cgcgccgtcg tcgtcccgga ccagcgacac ggccagcagg
     1381 acccagacca ggcggcccgt ccgggtcacg tagcgcttct ccatcgtgta gctggacgcc
     1441 tcgccggcca gcaaccgctg gacgtgggcc atgtccgagt ccaggtcctc cgggtgggtg
     1501 atgtcctgga aggacaacgc catcagctcg tcctcctcgt agcccagcag gtcgcacagg
     1561 gcctggttgc aacgctggaa gctcccgtcg acgcccacga gcgccatgcc gatcggcgcc
     1621 gcggcaaagg ccgtctcgaa caacgtggtc acggcgcgca gctcgtcgtc ggccagccgg
     1681 cggctggtga tgtcctgcga gacggccacc gcgcccacga tctgctcgcc ctcgatcagc
     1741 ggcgcgatgt gggtcaggta ccagcccgtg gcatcggacg agctgtgctc gaacgtgcgc
     1801 ggctcaccgg ccagcgcggc gcggtagtgc ggctccagct cggcgtagga gtgcgcgggg
     1861 gcgacgtcgg caagccggcg gccggccagg gtcgcggggt cgtagccgtg gcggcgcagc
     1921 gccgggccgt cggccagcag caggcgcatg tcccggtcca cggcgaacac cgcggctccg
     1981 ggcagggcgg agaccgccgc ggctcggagg gactcggaag tgaaggtgtc ggaaagcatc
     2041 ggggcggatg caccttccca gaacgaggtg cctttacccg ataatcggca cccgtgccgt
     2101 ggcgcttgag ctcagcggtg cagcttgggc tcgattatgc ggtacgcctt gagtcccagg
     2161 cccaggcgct cgcggtcctc cgaggtgaac ggatcgagcc cgctcagttc cttgacccgc
     2221 tccagccggt agctgaccgt gtgccggtgg gcgtggatcg ccgccgcggt ggcgttcatg
     2281 ttgcagttct gtcccaggta ggcctcgagc gtgccgacca ggtcggtcga gtactgctcg
     2341 tcgtagcggg ccagcggcgc gatcgtgtcc tcgtagaagc tgcgcacctc ctcggggtgg
     2401 ctggccagca cacggaacag cagccggtag gtaccgccgc cgatctcgtc gccgggggga
     2461 cctcccccgg cggcggtcac gcccagcacc agctcggcct cctccagcgc ccggcgcacg
     2521 tcggcagggt ccgagtagtg cgaggacagg ccgatcaggg cctggcggcc gaggcggttg
     2581 gccacctgtc gggcgacgtc caccgggccg ggcagcaggg cgtacacgcg cccggtgcca
     2641 cccggcgtgg ggatcacctg ggccagcgcc cccggcatct cgctggagat ggccgccagc
     2701 aggcgtcccg gggcgcgctc gccgggatcg gcgcagaggc cgaccgcgcc ctcgctgagg
     2761 tcacagccca gccggcgcgc gcggcggacg atgtcgcccg ggtccaggtc ctcccgggtc
     2821 agcagctcct cgaggaagga gccgcgaagg ttctgctcgg tctcgtcgcg ggcctcggcg
     2881 acggccacct cggtcagcgc ggccacggcg gccacgtgca ggtactcccc cgcgtccgag
     2941 ccgccggcgc ccagcagcag cacggcgccc agctcctggc cgccggagac gatcgggacc
     3001 tcggcggtga cctcgggcgg acggtcgggc ttgcccccgg ccagccgctt ggccacgtag
     3061 cgctcgtacg gggcccactc ctcggagggg gtccccagcc ggggcacgac cacggccacc
     3121 ggagcgccgg cgtgctgccc ggcgatctcg gccacccgct ccaggccgtc tccggccagg
     3181 acggcgccga ccatctccag atgtgcccct cgcggggagg gaagggattc gggtgcagtc
     3241 atggccatgg tgtcccccgg gaggaacgcg gcgtggccgg gcggcgttcc ctctcgggag
     3301 ccgcccggcc acgttccgca ggagggatca ggcccccgcc gggatctcct ccgtctcgtg
     3361 ggcctggagg acctcgcctc cgtcttcacc ggtgcggatg cggatggccg cctccaccgg
     3421 gatgacgaag atcttgccgt ctccgacctg acccgttctg gcgtgcttga ggatcgtctc
     3481 cgtgatcacg ccgacatccg tgtcgtcgac cacgatctcg agcttgagct tcgggcgcag
     3541 gtggatggtc aactcggagc cgcggtaccg ctccgtgatg cccttctgcc tgcccgagcc
     3601 cttgtactcc gcgatggaaa gactgggaaa gcccttgtcc agtagctcca tgcggatcgg
     3661 ctcgaacgcc tcgtggcgaa cgaacgcctc taccttcttc atgctgtcac ccccccgggc
     3721 acggcgtctc ctctgccctg atgggccacc ggagcctcac cggcgtgcgg cgcggccggt
     3781 ccggtcgagg cggcacccgt ctcgggcagc gggatgaact gctcggggta gccgtacatg
     3841 ccgtgctcga cgatgtcgag gccggcatcc tcttcctctt cggtcacgcg cagcccgatc
     3901 gtcttcttga tcaggaagaa cgtgccgtag ctgagcgcga acacgaacgt gaaggcgatc
     3961 agcacgccga ccacctgggc gatcagctgg tccgggccac cgccgtacag cagcccgccg
     4021 tcggggttgc cgaaggcgtt gtactgggcc aggcgcggtg acgtgaagaa gccgcaggcg
     4081 atggtgcccc agatgccggc caggccgtgc gccgaggttg caccgatcgg gtcgtcgatc
     4141 ttcttgtcga tcaggatcac gccgaacacg acgatcatgc ccgcgactgc accgatgatc
     4201 ggtgcggccc agacctccac gtagcccgag ggcgccgtga tgccgaccag gccggcgatc
     4261 atgccgttgc cgaccatccc gatgtcgatc gtcttcagcc tcagcatcga gaccgtgaac
     4321 gcaccgagca caccgcccgc cgcgcccagc atggtgatca cggcgacgtc cgcgaggcgt
     4381 ccgtcggcgg tacccagggt cgagccggcg ttgaagccga accagcccac gaacaggatc
     4441 aggacgccga ggcccaccag cggcatcgag tgccccggga tgacccgcgg cttgccgtcg
     4501 gagccgtact tgcccttacg cgggccgagc agcaacagcg cggccagcgc gccggtggcg
     4561 ccgatgaggt ggacggcggt cgacccggcg aagtcctgca tgccggtcgg catcagcggc
     4621 gtgtcgccgt tctgcagcca gtcaccgccg aacacccagt gggagccgat cgggtagatg
     4681 atcgccgaga agacgatcgc gtacagcacg taggcgccgt acttgatgcg ctcgagcgtc
     4741 gaaccccaga cgatggccag ggagaccgcg cagaacgcga actggaacag gaacttcgcg
     4801 tcgatggacg cggccgagac ggacatcacg gggatgcccg agttgccggc caggtcgtcg
     4861 acgccaccgg ttcccgccac ctgcaggaag aagccgttgg agccgatgat cgacgccagc
     4921 gtcgagttgt cgccgaacac gtcgccgctg ccgaacgcga aggcgaagcc caccgcccag
     4981 taacagatgg ccgcgatcga gaggttggtc aggatcttgg ccacgatcgt tccggcgttc
     5041 ttggcccgga cgaacccgac ctccaggaac atgaagccgg cctgcatgaa cagcacgagg
     5101 atggccgcaa tgaccaccca catggtgtcg atgggcaggg ttccctggtc gacgccatcg
     5161 acggcgttct tcagttcggc ggatgaagcc agcgacgtcg ccggcagaat gagcatggcc
     5221 aacagcgcca ccagggcgag agggccagcc cgcttaaggg atctcatcgt tgcgagttcc
     5281 tcctagtacc ccggcggcat gagaccaccg gatcgcagag tcaaagggga ctcaggcaac
     5341 ctaggtccgg gtgggccgtg gatctttatc caggtgtcga ggattcaggg ggcgtgtttc
     5401 tcccacctgt cgagccgggc ttggcccacc ggtcggattc cgctctggaa aggggaatca
     5461 gcgcggtcgg acgatgatgg tgcgaccgtc ctcgagccgg tagacggtgc ccagccggcg
     5521 gcccttgccc accacccggt cgccctcgcg caggcggatc ggccccgcgc gctcgccctt
     5581 gcgctggacc acctggtcgc ggctctgctg ctggcgtgcc tgcgccttgt tctcgcgcag
     5641 caccgacggg ctgagcttga ccgacgacag cgcggccagc aggaacgcca ccgccaggac
     5701 gatgcccagg tcgaacaggt tgaccaggcc gtccagcggg tcgcccccgc ggtcctcgcg
     5761 ggtccgcgcg cgcgggctca cgcgggaggc gctcacgccg gcaccggctc gggcgcgggc
     5821 gctccctcca gcgaggccag cacgtactcc acgtcggaga agtcctgcgc gtacaggcgg
     5881 tcccggacca gcgacacgcc gaacgcgatc acgcccacga ccaggccggc cacggtcacg
     5941 ctgaaggcca cccggaggtc gttgctcagc tgctccacgt tgccctgcgc cagcccctcg
     6001 agcgcgggcg acagggggat cagggtcccc atcaggccga ccgcggggcc ggtccggacc
     6061 aggatccggg tgcgctccaa gcgcttgagc gaccagtagt cgtactcggc catccgcttg
     6121 gcgatccggt cggcggcgcg gtcggcgtcg cgctgctcca cgatcgccgc cagcgcgtcg
     6181 cccatgcgct cgttgtagcc caggccgcgc gcggtgcgcg cggcgcccgc cctgtccccc
     6241 gtggccagcc gggcgcggat ggcggtgatg ccctcctcca ggcgcagcac gccgcggccg
     6301 cggcggcgca ccagctcggc ggccagcgcg cccgcctcga ccagcacgaa ggcgatcgag
     6361 gccagggcca gcaccaggac ggggatccgc agcgcctccg ccacgtcgaa gatcacgcgc
     6421 tccacgccgt tcaccgcggc caggggcagc aacgcgctcc cagcgccctc cgcggccagg
     6481 gcagcaccgt gcggagctcg atcacggcgc ccgtcccggc caggccgagg gtgaccagca
     6541 ggccccaggc cgccgtgggg atcccggggc cgggctcgtc gccacggtcg ttgccggtgc
     6601 gcgccgcgcg cttgcgggcg gcaggccggg gcgggaccac ggtggcgtcg gccagcaggg
     6661 tgccctcgac ctggcggcct cccggctgcg gcgccgggcg tgaccggcgc ggggtcgcgg
     6721 ccgggcccgc tgcgggtgcc cgggccgggc cgctc
//
LOCUS       DASUPW010000319         3374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1186038, whole genome shotgun sequence.
ACCESSION   DASUPW010000319 DASUPW010000000
VERSION     DASUPW010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186038"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3374
                     /locus_tag="VFY44_07155"
     CDS             <1..>3374
                     /locus_tag="VFY44_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX5910255.1"
                     /translation="TSVLVERFVQAVLEDGCAVDSMLAITFTEKAAAQLTARVRRRFL
                     ELGDEERAREAEAAWISTIHGFCARLLRTHALAAGLDPEFRVLDALEAERLAIDAFDK
                     ALGDFVGTGQDPGRLALLAAYTPDKLADMVRTAYGHLRSQGQRCPRLPEMTPPRDSGQ
                     RRALLEAVGPALAEIGAAEGTRAVEAVGRLDRCTAVLEALGPDDVHEPAELDKLKVGT
                     GANVLKGPACDAYREAYDAYLAFCLHYREHNDHVLVRELMALHDGHYAELKRGRSGLD
                     FDDLELVARDLLRAHPGIRDQYRERFSHVMVDEFQDTNPLQNELLELLEHGNLFRVGD
                     ERQSIYGFRHADVRVFRRHLAEARAQGRAERMTVNFRSRGEVLDAVDLTFEGLWGDDF
                     DALVERPGARDQPPRAEPCVELLVTDRHKDRWEERFPPGDGGEAKPFGDRLGSTEATI
                     WRAAEARLLAKRIDELAGPGRPFAYSDCVMLLRATTHMGVYERALEDRGVPTHVLGGR
                     GYWAQQQVADLRAYLAALANPLDELALHSVLASPLGGLSLDTLVVLATRSKCSGPGGI
                     WRVMTEDEELPGLIGGQQLELLRAFCDRFREDRVMAPRLSLETLIDRAVTRSGYDRHL
                     LALPAGDRRMANVRKLMRMAREFEADEGRDLRRFIDFVAERDLVQAGEGQAPLEAEDL
                     DAVRLMTIHRAKGLEFPLVCVADLGKTGREDDSALRVTDDGRLGMRLASLSGVSVDSA
                     EMERIKQEQKAADEEEERRIFYVAATRAEEHLVLSGATDLVKLQPEADLTEPMRWLWR
                     ALAPGLEEMGPEEVSVVEYEGRELRVACRVLRPGDVDVLLPEADRLPVAPEPEPPGLE
                     ALTAPALAAVPVPEALPVSRLSYSGLAAYRRCGYRFYLERALRLPPPDDGPARAARGG
                     DAGDAMPATVRGTVVHQLLEEIDFTAPAVPGAERVGQVIEALGQPVRAEEVEDVTGLV
                     AGVVGSALAQRIGAAERVHAELPFAFTLDTGGGRSLLVDGIVDVHAREGDAVLVVDYK
                     SDRLEGAEPAERVEGAYAIQQLVYALAALRSGAARAEVAYCFLERPDDPVTRVYEAAE
                     AGALEAELRELTAGVSEGRFEPTAHPHRGLCADCPG"
BASE COUNT          421 a         1206 c         1322 g          425 t
ORIGIN      
        1 gacctccgtg ctggtggagc ggttcgtgca ggccgtgctc gaggacggct gcgcggtcga
       61 ctcgatgctg gccatcacct tcaccgagaa ggccgcggcc cagctgaccg cgcgcgtgcg
      121 gcggcggttc ctggagctgg gtgacgagga gcgcgcgcgg gaggcggagg ccgcgtggat
      181 ctccaccatc cacggcttct gcgcccgcct tctgcgcacg cacgcgctgg ccgcggggct
      241 ggacccggag ttccgggtgc tggacgcgct ggaggccgag cggctggcca tcgacgcgtt
      301 cgacaaggcg ctcggcgact tcgtcggcac cggccaggac ccgggccggc tggcgctgct
      361 ggccgcgtac acgccggaca agctggccga catggtgcgc acggcctatg ggcacctgcg
      421 cagccagggc cagcgctgcc cgcggctgcc ggagatgacg ccgccgcgcg actcgggcca
      481 gcgccgggct ctgctggagg ccgtcggccc ggcgctggcg gagatcggcg ccgccgaggg
      541 cacccgcgcg gtcgaggcgg tcggccggct ggaccggtgc acggcggtgc tggaggcgct
      601 ggggccggac gacgtgcacg agccggccga gctggacaag ctcaaggtgg ggaccggcgc
      661 caacgtgctc aagggcccgg cgtgcgacgc ctaccgtgag gcctacgacg cctatctggc
      721 gttctgcctg cactaccgcg agcacaacga ccacgtgctg gtgcgagagc tgatggcgct
      781 gcacgacggc cactacgccg agctcaagcg cgggcgctcg ggcctggact tcgacgacct
      841 ggagctggtg gcgcgcgacc tgctccgcgc ccatcccggg atccgcgacc agtaccgcga
      901 gcgcttctcg cacgtgatgg tcgacgagtt ccaggacacc aacccgctgc agaacgagct
      961 gctggagctg ctcgagcacg gcaacctctt ccgcgtcggc gacgagcgcc agtccatcta
     1021 cggcttccgc cacgccgacg tgcgggtgtt ccggcgccac ctggccgagg cgcgggcgca
     1081 gggccgggcc gagcggatga cggtgaactt ccgcagccgt ggcgaggtcc tggacgcggt
     1141 cgacctgacc ttcgaggggc tctggggcga cgacttcgat gcgctggtcg agcgcccggg
     1201 ggcccgcgac cagcccccac gcgccgagcc gtgcgtcgag ctgctggtga ccgacaggca
     1261 caaggaccgc tgggaggagc ggttcccgcc gggggacggt ggcgaggcca agcccttcgg
     1321 cgatcgcctg ggttccaccg aggcgaccat ctggcgggcc gccgaggcgc ggctgctggc
     1381 caagcggatc gacgagctgg ccggcccggg ccggccgttc gcgtactcgg actgcgtgat
     1441 gctgctgcgc gccaccacgc acatgggcgt ctacgagcgg gcgctggagg accgcggcgt
     1501 ccccacccac gtgctgggcg gccgcggcta ctgggcccag cagcaggtgg ccgacctgcg
     1561 cgcctacctg gcggcgctgg ccaacccgct ggacgagctg gcgctgcact cggtgctggc
     1621 ctcgccgctc gggggcctgt cgctggacac gctggtggtg ctggccacgc ggtcgaagtg
     1681 ctccggcccg ggaggcatct ggcgcgtgat gaccgaggac gaggagctgc cgggcctgat
     1741 cggcggccag cagctggagc tgctgcgcgc gttctgcgac cgcttccggg aggaccgcgt
     1801 gatggccccg cggctgtcgc tggagacgct gatcgaccgc gcggtcacgc gctcgggcta
     1861 cgaccgccac ctgctggccc tgcccgcggg cgaccggcgc atggccaacg tgcgcaagct
     1921 gatgcggatg gcgcgcgagt tcgaggccga cgagggccgc gacctgcgcc gcttcatcga
     1981 cttcgtcgcc gagcgcgacc tggtgcaggc gggggagggc caggcgccgc tggaggccga
     2041 ggacctggac gccgtgcggc tcatgaccat ccaccgggcc aagggcctgg agttcccgct
     2101 ggtgtgcgtg gccgacctgg gcaagaccgg ccgcgaggac gattccgcgc tgcgcgtgac
     2161 cgacgacggc cgcctgggca tgcgcctggc gtccctgtcc ggggtgtccg tcgacagcgc
     2221 cgagatggag cggatcaagc aggagcagaa ggccgcggac gaggaggagg agcggcggat
     2281 cttctacgtc gccgccaccc gggccgagga gcacctggtg ctcagcgggg ccaccgacct
     2341 ggtcaagctc cagcccgagg ccgacctgac cgagcccatg cgctggctgt ggcgggcgct
     2401 ggccccgggg ctggaggaga tggggcccga ggaggtctcg gtcgtcgagt acgagggccg
     2461 cgagctgcgc gtggcctgcc gggtgctgcg cccgggcgac gtggacgtgc tgctccccga
     2521 ggccgaccgc ctccccgtgg cgcccgagcc cgagccaccc ggcctggagg cgctgaccgc
     2581 gcccgccctg gccgccgtgc cggtgcccga ggcgctgccg gtcagccgcc tgagctactc
     2641 cgggctggcg gcctaccggc gctgcggcta ccgcttctac ctggagcgcg cgctgcggct
     2701 gccgccgccc gacgacggcc cggcccgcgc cgcccggggt ggcgacgccg gggacgcgat
     2761 gccggcgacc gtccgcggca ccgtggtgca ccagctgctc gaggagatcg acttcaccgc
     2821 cccggcggtg cccggcgccg agcgcgtcgg ccaggtcatc gaggcgctcg gccagcccgt
     2881 ccgggcggag gaggtcgagg acgtgaccgg cctggtcgcc ggtgtcgtgg gctcggcgct
     2941 ggcgcagcgc atcggcgccg ccgagcgcgt ccacgccgag ctgccgttcg ccttcacgct
     3001 ggacaccggg ggcgggcgca gcctgctggt ggacggcatc gtggacgtgc acgcccgtga
     3061 gggcgacgcg gtgctcgtgg tcgactacaa gagcgaccgg ctcgagggcg ccgagccggc
     3121 ggagcgcgtc gagggcgcct acgcgatcca gcagctggtc tacgcgctgg ccgcgctgcg
     3181 gtcgggggcg gccagggcgg aggtggccta ctgcttcctg gagcgcccgg acgatcccgt
     3241 gacccgcgtc tacgaggccg cggaggccgg cgcgctcgag gcagagctgc gcgagctgac
     3301 cgcgggcgtc tccgaggggc gcttcgagcc caccgcccat ccccaccgcg gcctgtgcgc
     3361 cgactgcccc ggcc
//
LOCUS       DASUPW010000320         3344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_908654, whole genome shotgun sequence.
ACCESSION   DASUPW010000320 DASUPW010000000
VERSION     DASUPW010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908654"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VFY44_07160"
     CDS             <1..660
                     /locus_tag="VFY44_07160"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5910256.1"
                     /translation="DRPEDLYPSEARFLPDLARDAASVLDVGCGAGGFAAVWKHFNPE
                     LAYTGIDASEALIEAAARLHPDSRFLVGDGAEPLPVEDRGHDVVAALGWLHLEPRWRD
                     ALPELWRATGSRLFFDMRLHAGQEDLEGRQRLALAGEWDGSTTIPYLAASWPEVAGRL
                     AALAPARIRAYGYEGTPADTVSGMSNVCFATFVLERGDGHGRPAVEAELPYAWPEEAG
                     R"
     gene            657..1433
                     /locus_tag="VFY44_07165"
     CDS             657..1433
                     /locus_tag="VFY44_07165"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910257.1"
                     /translation="MIAALLLGRGGSTGLPGKNTRLVLGRPMMAYPLIAAGRSSHVDR
                     VYVSTDDAEIARIGGEYGAELIERPPELCTPEALGEDAYKHGFHVIQERLAAEGEELE
                     MLALLFANAPTVSGELIDQGVETLRADPSMDSAVTVSRYNMWSPLRARKLEGDGTLQP
                     FVPFETFGDPATLNCDRDSQGDVLYADMGLSVVRPRCLENMEEGLLPQKWMGQRIAPI
                     ESWGGCDVDYEWQIPGVEFWLREHGMADAGEPVATGHGDG"
     gene            1420..2358
                     /locus_tag="VFY44_07170"
     CDS             1420..2358
                     /locus_tag="VFY44_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX5910258.1"
                     /translation="MATAEPTRVLLSVSSFGAGGDQDRRKLEDAGMEVLDNPHGRRLT
                     RDEIGELIGDVHALVAGTEPLDLPVLEQASRLRVISRVGVGLENVDLNAARSLGIVVR
                     NTPDAVTDPVAELTLAGMLSLLRHIGEMDRALRAGSWERKMGGLLRGKTVGIVGLGRI
                     GRRLTELLAPFEVTVLASDRAPDQRVAGELGATYVELDELLERSDIVTLHVPGMTRPL
                     IGADELARMQPHALLVNASRGGLVDEDALLAALHEGRLAGAYVDTFVDEPYTGPLTEL
                     EQVLVTPHAGSYAREARARMESEAVDNLLGALEEFR"
     gene            2355..3239
                     /locus_tag="VFY44_07175"
     CDS             2355..3239
                     /locus_tag="VFY44_07175"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5910259.1"
                     /translation="MRTLVVGGSGFVGSHVADALTDAGHEVTILDSAEGPWRRPEQGF
                     RAGDVTDLESVAGAVEGHEVVYNFAGIADIDEARRRPVDTVAVNVQGNVNVLEACRRA
                     GVRRYVFASSIYVFSESGSFYRVSKQSAELYVEEYQREHGLDYTILRYGTLYGPRADA
                     RNSVHRYLTQALTERRVAATGTGEELREYIHVEDAARLSVDVLEPEFENEQVVLTGQQ
                     SMRYRDLLGLIREIVGEDVTIELSEPEGEGSAHYALTPYHFRPRVARKLVANHYVDLG
                     QGLLDCLHEIHEETAPRP"
BASE COUNT          464 a         1163 c         1276 g          441 t
ORIGIN      
        1 gaccggcccg aggacctgta cccgtccgag gcgcgcttcc tgccggacct ggcccgggac
       61 gcggcttcgg tgctggacgt gggctgcggc gcgggcgggt tcgccgccgt gtggaagcac
      121 ttcaaccccg agctggcgta caccgggatc gacgcgtccg aggcgctgat cgaggcggcc
      181 gcgcggctgc acccggactc gcgcttcctg gtcggcgacg gtgccgagcc gctccccgtc
      241 gaggaccgcg gccacgacgt ggtcgccgcg ctgggctggc tgcacctgga gccgcgctgg
      301 cgcgacgccc tgcccgagct gtggcgggcc accggcagcc ggttgttctt cgacatgcgc
      361 ctgcacgcgg gccaagagga cctggaaggc cgccagcggc tggcgctggc gggcgagtgg
      421 gacggcagca cgacgatccc ctacctggcc gcgtcgtggc cggaggtggc cgggcgcctg
      481 gccgccctgg cccctgcacg catccgtgcc tacgggtacg agggcacacc ggccgatacc
      541 gtctccggga tgtccaacgt ctgtttcgcg acattcgtgc tcgagcgagg cgacggccac
      601 ggtcgccccg cggtggaggc cgagctgccc tacgcgtggc ccgaggaggc ggggcgatga
      661 tcgccgcgct gctcctgggc cggggcgggt cgaccggcct gccgggcaag aacacccgcc
      721 tggtgctggg ccggccgatg atggcctacc cgctgatcgc ggccgggcgc tcgagccacg
      781 tggaccgcgt ctacgtgtcg accgacgacg ccgagatcgc gcgcatcggg ggcgagtacg
      841 gggccgagct gatcgagcgc ccgcccgagc tgtgcacgcc cgaggcgctg ggcgaggacg
      901 cgtacaagca cggcttccac gtgatccagg agcggctggc cgccgagggc gaggagctgg
      961 agatgctggc gctgctgttc gccaacgccc ccacggtcag cggcgagctg atcgaccagg
     1021 gcgtcgagac gctccgcgcc gacccgtcca tggacagcgc ggtgaccgtg tcgcgctaca
     1081 acatgtggag cccgctgcgg gcccgcaagc tggagggcga cggcacgctc cagccgttcg
     1141 tgccgttcga gaccttcggc gacccggcca ccctgaactg cgaccgcgac tcccagggcg
     1201 acgtgctgta cgcggacatg ggcctgtcgg tggtgcgccc gcgctgcctg gagaacatgg
     1261 aggagggcct gctgccgcag aagtggatgg ggcagcggat cgccccgatc gagtcgtggg
     1321 gcggctgcga cgtcgactac gaatggcaga tccccggagt ggagttctgg ctccgcgagc
     1381 acggcatggc cgatgccggc gagcccgtcg cgacgggtca tggcgacggc tgagcccacc
     1441 cgcgtcctgc tgtcggtctc gtccttcggc gcgggcggcg accaggaccg gcgcaagctc
     1501 gaggacgccg gcatggaggt gctggacaac ccgcacggac ggcgcctgac ccgcgacgag
     1561 atcggcgagc tgatcggcga cgtgcacgcg ctggtggccg gcaccgagcc gctggacctg
     1621 ccggtgctcg aacaggcctc gcggctacgg gtgatctcgc gcgtcggggt cggcctggag
     1681 aacgtggacc tgaacgctgc ccgtagcctg ggcatcgtgg tgcgcaacac ccccgacgcc
     1741 gtgaccgacc cggtggccga actgaccctg gccggcatgc tgtcgctgct gcgccacatc
     1801 ggcgagatgg accgcgccct gcgcgccggc agctgggagc gcaagatggg cggcctgctg
     1861 cgcggcaaga cggtcggcat cgtcggcctg ggccggatcg ggcgacggct gaccgagctg
     1921 ctggccccgt tcgaggtcac ggtgctggcc agcgaccgcg cgcccgacca gcgcgtcgcg
     1981 ggcgagctgg gcgccaccta cgtggagctg gacgagctgc tggagcgcag cgacatcgtg
     2041 acgctgcacg tgccgggcat gacccggccg ctgatcggcg ccgacgagct ggcccggatg
     2101 cagccgcacg ccctgctggt caacgcctcc cgcggcggcc tggtggacga ggacgcgctc
     2161 ctggccgcgc tgcacgaagg ccgcctggcc ggcgcctacg tggacacctt cgtcgacgag
     2221 ccctacaccg gcccgctgac cgagctcgag caggtgctgg tcaccccgca cgccggctcc
     2281 tacgcccgcg aggcgcgggc gcggatggag tccgaggcgg tggacaacct gctgggcgcg
     2341 ctggaggagt tccggtgagg acgctggtgg tggggggctc ggggttcgtg ggctcccacg
     2401 tggccgacgc cctgaccgac gcggggcacg aggtcacgat cctggacagc gcggagggcc
     2461 cgtggcgccg gcccgaacag ggcttccggg ccggtgacgt gaccgacctc gagtcggtgg
     2521 ccggcgcggt cgagggccac gaggtggtct acaacttcgc cggcatcgcc gacatcgacg
     2581 aggcccgccg gcgcccggtc gacacggtcg cggtgaacgt gcagggcaac gtcaacgtgc
     2641 tggaggcctg tcgccgggcc ggcgttcggc gctacgtctt cgcctccagc atctacgtgt
     2701 tcagcgagtc ggggtcgttc taccgcgtgt ccaagcagag cgccgagctg tacgtcgagg
     2761 agtaccagcg tgagcacggg ctggactaca cgatcctgcg ctacgggacg ctgtacggcc
     2821 cgcgggccga cgcgcgcaac agcgtgcacc gctacctgac ccaggcgctg accgagcgcc
     2881 gcgtggccgc cacgggcacg ggcgaggagc tgcgcgagta catccacgtc gaggacgccg
     2941 cgcggctgag cgtggacgtg ctggagccgg agttcgagaa cgagcaggtg gtgctgaccg
     3001 gccagcagag catgcgctac cgcgacctcc tgggcctgat ccgggagatc gtgggcgagg
     3061 acgtgaccat cgagctctcc gagcccgagg gcgagggctc ggcccactac gcgctcacgc
     3121 cctaccactt ccgcccgcgg gtggcgcgca agctggtggc caaccactac gtggacctcg
     3181 ggcaggggct gctggactgc ctgcacgaga tccacgagga gaccgcgccc cggccgtgat
     3241 ccgggcggcg atcctggact tcgacggcgt cgtcctcgag tccgccggca tcaagacgcg
     3301 ggcgttcgag cggctgttcg acggcgacga gcgcgcggtg gccc
//
LOCUS       DASUPW010000321         5647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_908903, whole genome shotgun sequence.
ACCESSION   DASUPW010000321 DASUPW010000000
VERSION     DASUPW010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5647
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908903"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VFY44_07180"
     CDS             complement(<1..332)
                     /locus_tag="VFY44_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910260.1"
                     /translation="MARQGYVNLAPAGSPEGDDAAMVAARAEFLGDGWFDPLSAALAA
                     AAAESPPPGAVAELGAGTGRHLAAVLDALGDDRAGVALDASVYAARRAARAHPRATSV
                     VADTWARLP"
     gene            complement(422..1090)
                     /gene="rpe"
                     /locus_tag="VFY44_07185"
     CDS             complement(422..1090)
                     /gene="rpe"
                     /locus_tag="VFY44_07185"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX5910261.1"
                     /translation="MLDQRRICPSILAADFAHLGDQVAAVMDAGARVIHVDVMDGHFV
                     PPISIGAAVTGALDAQVHGAGGWLDVHLMVDRPERHVAEFADAGADSISVHFEATPHI
                     HFALKAIREAGCHAGLALNPGTPLSAVEALHGSFDHLLCMTVNPGWGGQKWIPQSGER
                     IDSLRSLLPGDVPIQVDGGVDADTAVTAVTAGAGLLVAGSSVFGAADPAAAYSDLARA
                     AGAT"
     gene            complement(1172..2089)
                     /locus_tag="VFY44_07190"
     CDS             complement(1172..2089)
                     /locus_tag="VFY44_07190"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX5910262.1"
                     /translation="MAKNFLGDGGRDTFTGTPEADTINGRGGPDRLAGRGGDDTIFGG
                     TGYDRIFAGNGNDKAGGGPGRDVVRGGNGDDALAGGGGVRGRRADLLFGNAGTDTVNG
                     GPGWDFIAGNLGNDTLNGGGGKDRIFGGKGDDIVNGGPGAPFSRKRFEDRLHGGDGND
                     KVNGGNGRDYISGGAGDDISTGGNGADRIFANLGADQSDGGDGADDLWALARGDVSAP
                     GDPNGDALTGGNGADTFHTRDGEVDRIDCGNGKDVALLDQYDAIVDATPANANGSCER
                     VVRSSVLESDAEENKAGNPSEDKKEDAKL"
     gene            complement(2263..3858)
                     /locus_tag="VFY44_07195"
     CDS             complement(2263..3858)
                     /locus_tag="VFY44_07195"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX5910263.1"
                     /translation="MDYPVESFSESERAALAPHFTNLDSPVFGLVDLPETVKGALFAR
                     YSRYQGTLRRLYLDEFAADSSGQVRPFDGAEGERAAKLYERIFLGYGDDSVAQLGGAH
                     IACEYVSNVMTKVLQRGRLASYLEQSTRYIPYDAQMEGTGGYRYWRNPELGPEYARAM
                     DLLFDTYSRMLPQVEAWAADRFPRGDDENEAAHARSIRAKALDLLRGLLPAASLSHMG
                     IFASGQAYEQMILRLVASPLPEARDYGLMILNELKAMMPSFVSRVERSDRGGEWINYL
                     EERNRAADRWAGRLGLDRTRGGQEEAGPSVRLLDVQGSEDELLAALLFEAAGTPESEA
                     RAAVRTLPPDERAQMLGELVGERRNRRHRPGRGFESLRYRFEVVSDYGAFRDLQRHRM
                     LTVQWQTLTPDLGAGVPGELDEAGVGDDYRRALDASRGEYTRLVDAGLGHLAPYALCL
                     GYRIRYVLDMNAREAMHLIELRSGREGHPGYRAVAHELHAQIADVHPAVAAAMTFVDA
                     DTEPRLERILSEIRTQARQAAAH"
     gene            complement(3924..5567)
                     /locus_tag="VFY44_07200"
     CDS             complement(3924..5567)
                     /locus_tag="VFY44_07200"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX5910264.1"
                     /translation="MADLVLGPLLRYVDQDSAVVWVETSAPCEVRVLGSTAPTFSVKD
                     HHYGVVQIRDLKPGTTYEYGVELDGESVWPLADSELPGSFIRTYTDDIHPTIAFGSCR
                     VAVPHEPPYSLTKDEDDRGREIDALRTTALQMRDQDPSTWPDLLLWVGDQIYADEVSP
                     ETKEFIAQRRDTTEEPGEIALDFEEYCKLYYESWNEPVTRWLLSTVSSAMIFDDHDIH
                     DDWNTSAAWVEEIRKTDWWDDHIKAGLMSYWIYQHVGNLSPDEQDHDGLLGRLQHLTD
                     GTSVLREYARGSDREAGFSRWSFHRDVGRTRVVVIDSRCGRQLEEGNRSMLDEDEWQW
                     VEDHATGGFDHLLIATSLPWLLGQGMHHLEAWNEAVCAGAWGGLAAKLGEKIRQDVDL
                     EHWAAFNESFERLAEIQRAVGAGERGESPSSIVTLSGDVHHAYLSEVAFRRDANVTSA
                     VYQAVCSPMRNPLDARERRAIKAMFTRPVAAMMRALARSAGVTDPSIRWRRVDGGPYF
                     DNQLATLEIDGRRIDMKVEKALAPDGDPVLECVQQTRLA"
BASE COUNT          737 a         2007 c         2080 g          823 t
ORIGIN      
        1 ggcaggcgag cccacgtgtc ggccaccacg gacgtggcgc gcggatgagc gcgggcggcg
       61 cgccgggccg cgtagacaga ggcgtccagc gccacgcccg cgcggtcgtc gcccagggcg
      121 tccagcacgg ccgccaggtg ccggccggtg ccggcgccca gctcggccac cgcgcccggt
      181 gggggcgact cggccgccgc ggcggccagc gcggccgaga gcgggtcgaa ccagccgtcg
      241 cccaggaact cggcgcgcgc ggccaccatg gccgcgtcgt cgccctcggg ggagccggcg
      301 ggggccaggt tcacgtagcc ctggcgggcc acgtcgaacg agtggccctc ggcgcagccg
      361 aaggcgcgcg cgcccgcgtc cagctcgagc ccgcagaccg ggcaggccag cggcgggacg
      421 atcaggtcgc gcccgcggcc cgggccaggt cgctgtaggc cgcggccgga tcggcggcgc
      481 cgaacaccga cgagcccgcc accagcaggc ccgcgcccgc ggtcaccgcc gtcacggcgg
      541 tgtcggcgtc cacgccgccg tccacctgga tcggcacgtc gcccggcagc agcgagcgca
      601 gcgagtcgat ccgctcgccc gactgcggga tccacttctg accgccccag cccgggttca
      661 cggtcatgca cagcaggtgg tcgaacgagc cgtggagcgc ctcgaccgcg gagagcgggg
      721 tgcccgggtt cagcgccagg cccgcgtggc agcccgcctc gcggatcgcc ttcagcgcga
      781 agtggatgtg cggcgtggcc tcgaagtgca cggagatgga gtcggccccg gcgtcggcga
      841 actccgctac gtggcgctcg gggcggtcca ccatcaggtg cacgtccagc cacccgccgg
      901 cgccgtggac ctgggcgtcc agggcgcccg tgaccgcggc gccgatcgag atcgggggca
      961 cgaagtggcc gtccatgacg tccacgtgga tgacccgggc cccggcgtcc atgacggcgg
     1021 cgacctggtc acccagatgg gcgaagtcgg cggccaggat cgaggggcag atgcggcgct
     1081 ggtcgagcac ggggcgacca tacaacgacg aagggccggc atcgtgccgg cccttcgccc
     1141 gaaagctgac gctgtcagct gatgctcttg ctcagagctt cgcgtcttcc ttcttgtcct
     1201 cggacgggtt ccccgccttg ttctcctcgg cgtcggactc cagcaccgag ctgcgcacga
     1261 cgcgctcgca cgagccgttc gcgttggccg gggtggcgtc cacgatggcg tcgtactggt
     1321 cgagcagcgc gacgtccttg ccgttgccgc agtcgatccg gtcgacctcg ccgtcgcggg
     1381 tgtggaacgt gtccgcaccg ttgccgccgg tcagggcgtc gccgttggga tcgccggggg
     1441 cggacacgtc gccgcgggcc agggcccaca ggtcgtccgc gccgtcgccg ccgtcggact
     1501 ggtcggctcc caggttggcg aagatgcggt ccgcaccgtt gccaccggtc gagatgtcgt
     1561 cgccggcgcc gccggagatg tagtcccggc cgttgccgcc gttgaccttg tcgttgccgt
     1621 cgccgccgtg caggcggtcc tcgaaccgct tgcggctgaa cggggcaccg gggccgccgt
     1681 tgacgatgtc gtcacccttg ccgccgaaga tgcggtcctt gccgcctccg ccgttcaggg
     1741 tgtcgttgcc caggttgccg gcgatgaagt cccagccggg accgccgttg accgtgtcgg
     1801 tgcctgcgtt gccgaacagc aggtcggcac ggcgcccacg gacgccgccg ccgccggcga
     1861 gggcgtcgtc gccgttaccg ccacggacca cgtcgcggcc cgggccgccg ccggccttgt
     1921 cgttgccgtt cccggcgaag atccggtcgt agccggtgcc gccgaagatg gtgtcgtcgc
     1981 cgccgcgccc ggccaggcgg tcggggccac cgcggccgtt gatggtgtcg gcttcggggg
     2041 tgccggtgaa cgtgtcacgg ccgccgtcgc ccagaaagtt ctttgccatc gcgctcgccg
     2101 caaagacgca cgcgaatgca gcaagaatgc tcaggctgag cccgatcctt ccgctacgct
     2161 gcttcacagg ggttgcctcc tacttggtgg ttgggggttg cccctctaac tcccccgggg
     2221 cggccaaagt tgcggaccgc tcgacagatc gcggaatccg tgttaatgcg cggcggcttg
     2281 gcgtgcctgc gtgcggatct cgctgaggat gcgctcgagc cggggctcgg tgtccgcgtc
     2341 gacgaacgtc atggcggcgg ccaccgccgg gtgcacgtcc gcgatctggg cgtgaagctc
     2401 gtgcgcgacg gcccggtagc cgggatgccc ctcgcgcccc gagcgcagct cgatcaggtg
     2461 catggcctcg cgggcgttca tgtccagcac gtaccggatc cggtacccca ggcagagcgc
     2521 gtacggcgcc aggtggccca ggcccgcgtc caccaggcgc gtgtactcgc cacgtgacgc
     2581 gtcgagggcg cggcggtagt cgtcgcccac accggcctcg tccagctcgc cgggcacgcc
     2641 ggcgcccagg tcgggcgtca gcgtctgcca ctgcacggtg agcatgcggt ggcgctggag
     2701 gtcgcggaac gcgccgtagt cggacacgac ctcgaaccgg tagcgcagcg actcgaaccc
     2761 gcggccgggg cggtggcggc ggttgcggcg ctcgccgacc agctcgccga gcatctgggc
     2821 gcgctcgtcg ggtgggagcg tgcgcacggc ggcgcgcgcc tcggactccg gcgtcccggc
     2881 ggcctcgaac agcagcgccg ccagcagctc gtcctcggag ccctggacgt cgagcaggcg
     2941 gaccgagggc ccggcctcct cctggccgcc gcgggtgcgg tccaggccga gccggccggc
     3001 ccagcggtcg gcggcccggt tgcgctcctc caggtagttg atccactcgc cgccgcggtc
     3061 ggaccgctcg acgcgcgaga cgaagctggg catcatcgcc ttcagctcgt tcaggatcat
     3121 caggccgtag tcgcgcgcct cgggcagcgg cgacgccacc agccgcagga tcatctgctc
     3181 gtacgcctgg ccgctggcga agatgcccat atgcgacagc gacgccgcgg gcagcaggcc
     3241 gcgcagcagg tccagcgcct tggcgcggat cgaccgggca tgcgcggcct cgttctcgtc
     3301 gtcgccgcgc gggaaccggt ccgcggccca ggcctcgacc tgcggcagca tgcgcgagta
     3361 ggtgtcgaac agcaggtcca tggcccgcgc gtactcgggg ccgagctcgg ggttgcgcca
     3421 gtagcggtag ccgcccgtgc cctccatctg cgcgtcgtag gggatgtagc gcgtcgactg
     3481 ctcgaggtag gacgccagcc ggccgcgctg gagcaccttg gtcatcacgt tcgacacgta
     3541 ctcacaggcg atgtgggcgc cgcccagctg ggccaccgag tcgtccccgt agcccaggaa
     3601 gatgcgctcg tagagcttgg ccgcgcgctc gccctcggcg ccgtcgaagg ggcggacctg
     3661 gccgctcgag tcggcggcga actcgtccag gtacagccgc cgcagggtgc cctggtagcg
     3721 cgaatagcgg gcgaacagcg cccccttgac cgtctccggc aggtccacca ggccgaatac
     3781 gggggagtcg aggttcgtga agtgcggtgc cagcgcggcg cgctcggatt cgctgaagct
     3841 ctcgacggga tagtccatgc gggtgggcca gcatagtccc ggctccggcg ggcccggagc
     3901 ccggtcccgc aaactgcgga gacctacgcc agccgcgtct gctgcacgca ctccaggacg
     3961 ggatcgccgt ccggggccag cgccttctcg accttcatgt cgatccggcg cccgtcgatc
     4021 tccagcgtgg ccagctggtt gtcgaagtag ggccctccgt ccacgcggcg ccagcggatc
     4081 gaggggtcgg tgacgccggc ggagcgggcc agcgcgcgca tcatcgccgc caccgggcgc
     4141 gtgaacatgg ccttgatggc gcgccgctcg cgcgcgtcca gcgggttgcg catcggcgag
     4201 cacaccgcct ggtagaccgc gctggtcacg ttggcgtcgc ggcggaaggc gacctcgctc
     4261 aggtacgcgt ggtgcacgtc gccggacagg gtcacgatcg acgaggggct ctcgccgcgc
     4321 tcgccggcgc ccacggcgcg ctggatctcg gccagccgct cgaacgactc gttgaaggcg
     4381 gcccagtgct ccaggtccac gtcctggcgg atcttctcgc cgagcttggc cgcgagcccg
     4441 ccccaggcgc cggcgcagac ggcctcgttc cacgcctcca ggtggtgcat gccctggccg
     4501 agcagccacg gcagcgaggt ggcgatcagc aggtggtcga agccgccggt ggcgtggtcc
     4561 tcgacccact gccactcgtc ctcgtccagc atcgagcggt tgccctcctc gagctggcgc
     4621 ccgcagcgcg agtcgatgac caccacgcgg gtgcggccga cgtcgcggtg gaagctccag
     4681 cggctgaagc cggcctcgcg gtccgagccg cgggcgtact cgcgcaggac cgaggtgccg
     4741 tcggtcaggt gctgcagccg gcccagcagc ccgtcgtggt cctgctcgtc ggggctgagg
     4801 ttgcccacgt gctggtagat ccagtacgac atcagcccgg ccttgatgtg gtcgtcccac
     4861 cagtccgtct tgcggatctc ctccacccac gcggcggagg tgttccagtc gtcgtggatg
     4921 tcgtggtcgt cgaagatcat ggccgacgac accgtggaca gcagccagcg cgtgaccggc
     4981 tcgttccagg actcgtagta gagcttgcag tactcctcga agtccagcgc gatctcgccc
     5041 ggctcctcgg tggtgtcgcg ccgctgcgcg atgaactcct tcgtctcggg cgacacctcg
     5101 tcggcgtaga tctggtcgcc gacccacagc aggaggtcgg gccaggtgga ggggtcctgg
     5161 tcgcgcatct ggagcgcggt ggtgcgcagc gcgtcgatct cgcggccgcg gtcgtcctcg
     5221 tccttggtaa gcgagtaggg cggctcgtgg ggcaccgcga cgcggcagga cccgaaggcg
     5281 atcgtggggt ggatgtcgtc cgtgtaggtg cggatgaagc tgcccggcag ctccgagtcg
     5341 gccagcggcc acaccgactc gccgtccagc tcgacgccgt actcgtacgt ggtgccgggc
     5401 ttgaggtcgc ggatctggac caccccgtag tggtggtcct tcacggagaa cgtgggcgcg
     5461 gtcgagccca gcacgcgcac ctcgcagggc gcgctggtct cgacccacac gaccgcgctg
     5521 tcctggtcga cgtagcgcag gagtggtccg aggacgagat cagccatcgc gcaggtggta
     5581 cccgatctgg cgggtgggcg gtattaagcg ttggttggga agggccggtc agcggaggct
     5641 gcgcacg
//
LOCUS       DASUPW010000322         2938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_2018219, whole genome shotgun sequence.
ACCESSION   DASUPW010000322 DASUPW010000000
VERSION     DASUPW010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2018219"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="VFY44_07205"
     CDS             complement(<1..1053)
                     /locus_tag="VFY44_07205"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX5910265.1"
                     /translation="MATRLVVSLLVAVLVAVAGPAQPARAAAPIPTATVAQSGLVIPW
                     DLAFAPDGTMFVTERPGRIRVYASGAPGAAIVSTTAVPNVRSEHESGLNGIAVDVNFA
                     SNRFIYVCASVDPAPAEPTNNDLNWTNQVMRYRVTAQRTLTERTVIFNGTLGNPARAA
                     FQHNGCSVEMDSSGKLWVGIGDARQPADAQDPESFNGKILRMNRDGTVPSDNPIMPGA
                     AARSQVYTMGHRNPQGIAFQPGTGRVYAAEHGPNENDEINLVQAGGNYGWPCYTGVAT
                     PFTPAGCNAANQYLAPAWTSGTPTIATSGITFLSHARWADWRGSAVVMQLKEQDVRRF
                     VPSANGAVMTMADLFFD"
     gene            complement(1133..1209)
                     /locus_tag="VFY44_07210"
     tRNA            complement(1133..1209)
                     /locus_tag="VFY44_07210"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1173..1175),aa:Glu,seq:ttc)
     gene            complement(1246..1557)
                     /locus_tag="VFY44_07215"
     CDS             complement(1246..1557)
                     /locus_tag="VFY44_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910266.1"
                     /translation="MHPMLRNTVIGVVGLIIAGGLTALALLGRDSGLSVLALLAAGVI
                     GALIGVFMYSQGWVWGSRAARQRDTGQAVLIAVGAGLMALVAAVAIAGLLVLALLFLI
                     E"
     gene            1690..1962
                     /locus_tag="VFY44_07220"
     CDS             1690..1962
                     /locus_tag="VFY44_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910267.1"
                     /translation="MAERSTADQRGTPESDGNMSVREAGRKGGKTTRARYGPEFFQEI
                     GAKGGKAVSERYSNEHFREIGRKGGRRVAELIERAKRMDEPPGQRE"
     gene            complement(1950..2552)
                     /locus_tag="VFY44_07225"
     CDS             complement(1950..2552)
                     /locus_tag="VFY44_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEX5910268.1"
                     /translation="MVFPWQKRTVPPELAARVPPGQVLTEKFPVLHFGRVPSYGDLTG
                     WDFRVWGEVAEPFSLRWAEFRNLPVTERTLDIHCVTRWSKLDTTWEGVALSHIAEVAQ
                     PTAAARYVIFHSEGGYTANVPLATAMQPECVLAWSFDGQPLAPEHGYPLRAIVPGTYF
                     WKSAKWLRGVEFTAEDHPGFWERNGYHNDADPWKEERYSR"
     gene            2610..>2938
                     /locus_tag="VFY44_07230"
     CDS             2610..>2938
                     /locus_tag="VFY44_07230"
                     /inference="COORDINATES: protein motif:HMM:NF016553.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910269.1"
                     /translation="MPPASTPFADLAAVAASVGSTRSKLEKRDRLAAYLRDLPADDLA
                     AAAVFFAGRPVQDPTAKLGLGWVQQGAALAAASGADDAALGAAYLRHSDFGDAAAELL
                     ARPSADG"
BASE COUNT          443 a         1009 c         1060 g          426 t
ORIGIN      
        1 gtcgaagaac aggtcggcca tcgtcatgac ggcaccgttg gccgatggga cgaagcggcg
       61 aacgtcctgc tccttgagct gcatcaccac ggcgctgccg cgccagtcgg cccagcgtgc
      121 gtggctcagg aacgtgatgc ccgacgtcgc gatcgtcggg gtgccggacg tccacgccgg
      181 ggcgaggtac tggttcgccg cgttgcagcc ggccggggta aagggcgtcg ccacaccggt
      241 gtagcagggc cagccgtagt tgccgccggc ctggacgagg ttgatctcgt cgttctcgtt
      301 cgggccgtgc tcggccgcgt agacgcgtcc ggtgccgggc tggaaggcga tgccctgcgg
      361 gttgcggtgc cccatggtgt agacctggct gcgggcggcg gccccgggca tgatcgggtt
      421 gtcggacggc acggtgccat cgcggttcat gcgcaggatc ttgccgttga agctctccgg
      481 atcctgggcg tccgcgggct gcctggcgtc accgatcccc acccacagct tgcccgagga
      541 gtccatctcc accgagcagc cgttgtgctg gaaggcggct ctggcgggat tgcccaatgt
      601 gccgttgaag atcacggttc gctcggtcag cgttcgctgg gcggtcaccc ggtaccgcat
      661 cacctggttc gtccagttga ggtcgttgtt ggttggctcc gcgggagcgg gatcgacgga
      721 ggcacagacg tagatgaacc ggttgctcgc gaagttcacg tcgacggcga tgccgttcag
      781 cccggactcg tgctcggatc ggacgttcgg taccgccgtg gtgctgacga tcgccgcgcc
      841 gggcgcgccg ctggcataga cccggatccg cccgggccgc tcggtgacga acatcgtccc
      901 gtccggcgcg aaggcgaggt cccacgggat gacgaggccg ctctgcgcga cggtcgcggt
      961 cgggatcggt gcggcggccc gcgccggttg ggcgggaccg gcgacggcga cgagaacggc
     1021 gacgagcagc gagacgacga gtcgcgtggc cacgggatgg gatctccccc tgcgcaggct
     1081 ggtgttccgc ggatcacctt acgtcatcga cgccggcgcg cgcgggaagt ggtggtgccc
     1141 ccaaggggat tcgaacccct gatctccacc ttgaaagggt ggcgtcctag gcctctagac
     1201 gatgggggcg acggcgcgga cgggccgcca tcgtagcagc gcccctcact cgatgaggaa
     1261 gaggagggcc agcaccagca gcccggcgat ggcgaccgcc gcgacgagcg ccatcagccc
     1321 ggcgcctacc gcgatgagga ccgcctgccc ggtgtcgcgc tgccgggccg cccgggagcc
     1381 ccacacccaa ccctggctgt acatgaacac gccgatgagg gcgccgatga cgccggcggc
     1441 gagcagggcc aggaccgaga ggccggagtc gcggccgagc aacgccaggg ccgtgagccc
     1501 tccggcgatg atcaggccca ccacgccgat cacggtgtta cgcagcatcg ggtgcatcgg
     1561 cgggccattg tacgtgaccc tcgatcggtc tccgttcgcc ctatccgatg gcgccggtac
     1621 gcatcttgct tccctgctgc gggacgtacg actcgactca ggcaacacag cagcaggagg
     1681 cgcgcaccga tggcggagcg aagcaccgcc gaccagcgcg gcacccccga aagcgacggg
     1741 aacatgagcg tccgggaagc cggacgcaag ggtgggaaga cgacgcgcgc ccgctatggc
     1801 ccggagttct tccaggagat cggtgccaag ggcggcaagg ctgtcagtga acggtactcg
     1861 aacgagcact tccgcgagat cgggcgcaag ggcggccgtc gcgtggcgga gctgatcgag
     1921 cgcgccaagc gcatggacga gcccccgggt cagcgcgagt agcgctcctc cttccatggg
     1981 tccgcgtcgt tgtggtaccc gttgcgctcc cagaagccgg ggtggtcctc cgcggtgaac
     2041 tccacgccgc gcagccactt cgcgctcttc cagaagtacg tgcccggcac gatggcccgc
     2101 agcgggtatc cgtgctccgg tgccagcggc tggccgtcga acgaccacgc cagcacgcac
     2161 tccggctgca tcgccgttgc cagcgggaca ttggcggtgt atcccccctc cgagtggaag
     2221 atcacgtacc gcgccgcggc ggtgggctga gccacctcgg cgatatggct gagggccacg
     2281 ccctcccagg tggtgtcgag cttcgaccag cgtgtgacgc agtggatgtc gagcgtgcgc
     2341 tccgtcaccg gcaggttgcg gaactccgcc caccgcaggc tgaacggctc ggcgacctca
     2401 ccccacacgc ggaaatccca cccggtgagg tccccgtagc tcggcacgcg gccgaagtgg
     2461 agcaccggga acttctcggt caggacctgg ccgggcggca cgcgtgcggc gagctcggga
     2521 ggcacggtgc gcttctgcca cggaaagacc atgcgtgcag catcggcatc cgggcggccg
     2581 gggtcaagat cgctccgtag actggaccca tgccccccgc ctccacgccg ttcgccgacc
     2641 tggcggccgt ggccgcatcg gtcgggtcga cgcgcagcaa gctcgagaag cgcgaccggt
     2701 tggccgccta cctgcgcgac ctgcccgccg acgacctggc cgccgcggcc gtctttttcg
     2761 ccgggcggcc ggtgcaggac ccgacggcca agctcgggct gggctgggtg cagcagggcg
     2821 cggcactggc cgccgccagt ggggccgacg atgccgccct cggcgccgcg taccttcgcc
     2881 actccgactt cggcgacgca gccgccgagc tgctcgcccg cccgagcgca gacgggca
//
LOCUS       DASUPW010000323         2855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_494454, whole genome shotgun sequence.
ACCESSION   DASUPW010000323 DASUPW010000000
VERSION     DASUPW010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494454"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2386
                     /locus_tag="VFY44_07235"
     CDS             <1..2386
                     /locus_tag="VFY44_07235"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX5910270.1"
                     /translation="VVAVVAACLSSVPAASAETTRYILPPGNFGGLPTTQNSLDQLPL
                     YDGLTPLRGNVTPADVNRFYLPEDFNPIGATRDEPTGRAGLRIAYDTYGVPHIYGNTR
                     DDLSFGSGWVTARDRGLLLSLGRGPARVAVADVPGIDAFSLVTSGKGFAPSKQAEALV
                     TKQKNLLVKEYGAKGRQILKDAQAYIDGANAYQRSKGAAADYTVNDVIATTAFIGSIF
                     GAGGGAEAQNSQLLGALRKRFGNKRGTGAFKDVMLADDPEAPTTIGKTFKYGPTTGGK
                     VKGSVVVDPGSLKPFDPLKASKSVAKTDALAAPARRRASNFLITAPNRSATGRTLAVM
                     GPQLGYYYPEIVQQMHLQGAGINAQGAFVPGLAMYMLLGRTNNYAWSLTSAGHDVRDV
                     YALPLCGSKGKRAKRTSNRYRYKGKCRSFTSFNAGKLGGKELKFKKSVHGAVIGTATV
                     KRKAYAFARKRSTFGRDALNLAALKDMTEGKGSTPASFYKSANQFGFTFNWGYINRTA
                     TANFSSGRLPVRPKGLDRRLPTLGTGKYEWKGYLSQNQHPHDTSGPKGLLLNWNNRSA
                     PGFMHGDDEPYGSVQRVELFDKVPGKATLANMVGTMNRAATEDVRSPAWPSISKVLAS
                     GKAPNARAAQVKTILDGWVARDAPRLDATDDGTYDEPGPAIMDAAWNPMAKAVMRPRF
                     GKLLGQLDEVRSLGSLSGVSYVDKDLRTLLKQRVRGKFSQSYCGKGKLAPCRASLWKA
                     LDSASASLAKRYGPDPTKWLMKGERTTFVPGLIKDSFRRTNRPTFQQALEFDRR"
     gene            2443..>2855
                     /locus_tag="VFY44_07240"
     CDS             2443..>2855
                     /locus_tag="VFY44_07240"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5910271.1"
                     /translation="MRFGDRSLSYAELAAAAAAVVTEVGRVQRLAVWAEPTLETCVAV
                     AAAVGAGIPLVPMNPKLGHGELRHVLEDSRPDAILGAPDGALPGDLDHPPRRLAVDVD
                     RRGGEIQAHPRGPEDTALIVYTSGTTGAPKGAVLP"
BASE COUNT          462 a         1046 c          981 g          366 t
ORIGIN      
        1 ggtagtggcg gtggtcgccg cctgtctgtc ctccgtgccc gcggcgtcgg cggagaccac
       61 ccggtacatc ctgccgcccg gcaacttcgg tggcctgccc accacacaga actcgctgga
      121 ccagcttccg ctgtacgacg gcctgacccc gctgcggggc aacgtgaccc cggcggacgt
      181 gaaccgcttc tatctccccg aggacttcaa cccgatcggc gccaccaggg acgagccaac
      241 cggccgcgcc gggctgcgga tcgcctacga cacctacggc gtgccgcaca tctacggcaa
      301 cacccgcgac gacctctcgt tcggctccgg ctgggtgacc gcgcgtgacc gcggcctgct
      361 gttgagcctg ggccgcggcc cggcccgcgt ggccgtggcc gacgtgccgg gcatcgacgc
      421 cttctcgctg gtcaccagcg gcaagggctt cgcgcccagc aagcaggccg aggcgctcgt
      481 gaccaagcag aagaacctgc tggtcaagga gtacggcgcg aaggggcgcc agatcctcaa
      541 ggacgcgcag gcctacatcg acggcgccaa cgcctaccag cgctccaagg gcgcggcggc
      601 cgactacacc gtcaacgacg tgatcgccac gaccgcgttc atcggctcga tcttcggggc
      661 cggtggtggc gccgaggcgc agaactcgca gctgctcggc gccctgcgca agcggttcgg
      721 gaacaagcgc ggcaccggcg ccttcaagga cgtgatgctg gccgacgatc ccgaggcgcc
      781 gaccacgatc ggcaagacgt tcaagtacgg cccgaccacg ggcggcaagg tcaagggctc
      841 ggtggtggtc gacccgggct cgctgaagcc gttcgacccg ctcaaggcat ccaagagcgt
      901 cgccaagacc gacgcgctgg cggcgccggc gcggcggcgg gcgtcgaact tcctgatcac
      961 cgcccccaac cggtcggcca ccggcaggac cctggccgtg atgggcccgc agctgggcta
     1021 ctactacccc gagatcgtgc agcagatgca cctccagggc gcgggcatca acgcccaggg
     1081 cgcgttcgtg cccggcctgg ccatgtacat gctgctgggc cgcaccaaca actacgcctg
     1141 gagcctgacc tcggccggcc acgacgtgcg tgacgtctac gcgctgccgc tgtgcgggtc
     1201 caagggcaag cgcgccaagc gcacctccaa ccgctaccgc tacaagggca agtgccgctc
     1261 gttcacctcg ttcaacgcgg gcaagctcgg cggcaaggag ctgaagttca agaagtcggt
     1321 gcacggcgcc gtgatcggca ccgccaccgt caagcgcaag gcctacgcct tcgcccgcaa
     1381 gcgctccacg ttcggccgcg acgcgctgaa cctggccgcg ctgaaggaca tgaccgaggg
     1441 caagggcagc acgcccgcgt ccttctacaa gtccgccaac cagttcggct tcacgttcaa
     1501 ctggggctac atcaaccgca ccgccacggc caacttctcg tccggccggc tgccggtgcg
     1561 gccgaagggg ctcgaccgcc gcctgcccac gctgggcacc ggcaagtacg agtggaaggg
     1621 ctacctgagc cagaaccagc acccccacga caccagcggt cccaagggcc tgctgctgaa
     1681 ctggaacaac cgctcggcgc cgggcttcat gcacggtgac gacgagccct acggctcggt
     1741 ccagcgtgtc gagctgttcg acaaggtgcc gggcaaggcg acgctggcca acatggtcgg
     1801 caccatgaac cgggcggcca ccgaggacgt ccgctcgccc gcgtggccgt ccatcagcaa
     1861 ggtgctggcc tcgggcaagg cgcccaacgc ccgcgccgcc caggtcaaga cgatcctgga
     1921 cggctgggtg gcacgtgacg ccccgcgcct ggacgccacc gacgacggca cctacgacga
     1981 gcccgggccg gccatcatgg acgcggcctg gaacccgatg gccaaggccg tgatgcggcc
     2041 ccggttcggc aagctgctgg gccagctcga cgaggtccgg tccctgggca gcctgtccgg
     2101 tgtctcctac gtggacaagg acctgcgcac cctgctcaag cagagggtgc ggggcaagtt
     2161 cagccagagc tactgcggca agggcaagct ggctccgtgc cgcgcctcgc tctggaaggc
     2221 gctggactcc gcgtcggcct cgctggccaa gcggtacggg cccgacccga ccaagtggct
     2281 gatgaagggc gagcggacca cgttcgtccc ggggctgatc aaggacagct tccggcgcac
     2341 gaaccgcccg acgttccagc aggcgctgga gttcgaccgg cgctaggcct gcaccctgat
     2401 gcgctgatcc cgaacctgca cgagccgggc gatgccccgg ccgtgcggtt cggcgatcgg
     2461 tcgctcagct acgcggagct ggccgcggcg gcggccgcgg tggtgacgga ggtcggccgg
     2521 gtccagcgcc tggcggtgtg ggccgagccc acgctggaga cgtgcgtggc ggtcgccgcg
     2581 gccgtgggcg ccggtatccc gctggtgccg atgaacccga agctggggca cggcgagctg
     2641 cgccacgtgc tcgaggacag ccggcccgac gccatcctgg gcgcgccgga cggggcgctg
     2701 ccgggcgacc tcgaccatcc cccacgccgg ctcgcggtcg acgtggaccg ccgtggcggt
     2761 gagatccagg cgcacccgcg cgggcccgag gacaccgcgc tgatcgtcta cacctcgggc
     2821 accacgggcg cgccgaaggg cgcggtcctg cccca
//
LOCUS       DASUPW010000324         3380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_909338, whole genome shotgun sequence.
ACCESSION   DASUPW010000324 DASUPW010000000
VERSION     DASUPW010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909338"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2219
                     /locus_tag="VFY44_07245"
     CDS             <1..2219
                     /locus_tag="VFY44_07245"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5910272.1"
                     /translation="TRAVRPGPLSADAVAELVRERLGPNAEPEFCTACHSATGGNPLL
                     LGQLLTALEGDSVKPDAQNAGAVRDIGPRAVSRGVLLRLARLPGPAVEVARAVAVMGD
                     GADLATITAFTELDEATVAATSGDLARAEILAPDTPLSFVHPLVRDAVYEELSPAERE
                     LQHARAVDVMVAAGAGDEHVAAHLLLMAKRGDQLVVDRLVEAARAAVAQGAAEGAVAY
                     LRRAFDEPAPPEQRPQLLLELGTAEALASGAEAVEHLQAAYEQLDDPAQRIAAAPVLA
                     ESLIFRGRAGEAAAFTREAARHVPEEMAPMRAVLEGIELGTTMFGVAEPDMVRRLGEY
                     RDRPVGPGPGDKMIAAMTAWEWALTGGGADECVELARQALTDDSVVVAENGFLTVPAI
                     TVMAMADLDEAAERWDRLVAQTQASGSHFAVSCAHLWSGITQTRRGELAEAEALLRTG
                     RDELSMWGPLPVDGAYFSGTIPLVLLERGDVEAARRELGERPSRIYDHHDAGHLWLRC
                     ETEILLAEGRNEEALERSEEFGEVAGRLVNPAWVPWRSLQAIALDRLGRTGEAVTRAE
                     EELEHARRWGAPGTVGRSLRVLGTLRRAEGLELLDQAVALLERSPARLELAKALAAQG
                     ATLRRERKPTDARDPLRRALELAELCDAGGLAAEVRTELYATGSRPRSNALTGPEALT
                     ASEKRVAELAAEGLTNRDIAQTLFVTPKTVEVHLSATYRKLGIRSRRELAGSFAAA"
     gene            2253..2597
                     /locus_tag="VFY44_07250"
     CDS             2253..2597
                     /locus_tag="VFY44_07250"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5910273.1"
                     /translation="MSTSTSTILDLSVHETEGTLLVRLDGEFDLAGVGMVENALDRMS
                     RPPPLARVVFDLRELEFLDLAGLQTILRADARARAGGYEVVVVRPHGTANRVFTLTRA
                     GETLNMADRPPY"
     gene            complement(2607..2918)
                     /locus_tag="VFY44_07255"
     CDS             complement(2607..2918)
                     /locus_tag="VFY44_07255"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEX5910274.1"
                     /translation="MATCTHLDTVAITELPESVEGCTDCLATGGRWLHLRICLGCGHV
                     GCCDSSPNQHASKHFEESGHPLIRSLEPGEEWSFCFVDDAGMLLPDVHGSTRIPPAPL
                     G"
     gene            complement(2937..>3380)
                     /locus_tag="VFY44_07260"
     CDS             complement(2937..>3380)
                     /locus_tag="VFY44_07260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEX5910275.1"
                     /translation="DLAEVRLPFADGTDPDAATAEVLGRVDAAGAVLLATPVYRGSLT
                     GALKNLLDLLPIDTLEGKPVALLAMGASPHHFLGAERHLRDVLAFFGAHVVPVAGYLT
                     SRDFADGEPTDAAAESVDGLLAGLGALEDALAAAPTAGLRPLAAR"
BASE COUNT          430 a         1247 c         1309 g          394 t
ORIGIN      
        1 cgacccgcgc cgtgcggccc ggcccgctga gcgccgacgc ggtggccgag ctggtgcgcg
       61 agcggctggg cccgaacgcc gagcccgagt tctgcaccgc ctgccactcc gccaccggcg
      121 gcaacccgct gctgctgggc cagctgctga ccgcgctgga gggcgactcg gtgaagcccg
      181 acgcccagaa cgccggcgcg gtgcgcgaca tcggcccgcg cgcggtctcg cgcggcgtgt
      241 tgctgcgcct ggcccgcctg cccggccccg ccgtggaggt ggcgcgcgcg gtggcggtca
      301 tgggcgacgg cgccgacctg gccaccatca ccgcgttcac ggagctggac gaggccacgg
      361 tggcggccac ctcgggggac ctggcccggg ccgagatcct ggcccccgac acgccgctgt
      421 ccttcgtgca cccgctggtg cgcgacgccg tgtacgagga gctgtcgccg gccgagcgcg
      481 agctccagca cgcacgcgcg gtcgacgtga tggtcgcggc cggggccggc gacgaacacg
      541 tggcggcgca cctcctgctg atggccaagc gcggcgacca gctggtggtc gatcgcctgg
      601 tggaggccgc ccgcgccgcc gtcgcccagg gggcggccga gggcgccgtg gcctacctgc
      661 gccgggcctt cgacgagcca gcgccgcccg agcagcggcc ccagctgctt ctggagctgg
      721 gcacggccga ggcgctggcc agcggcgccg aggcggtgga gcacctgcag gccgcctacg
      781 agcagctgga cgatccggcc cagcggatcg cggccgcgcc ggtgctggcc gagtcgctga
      841 tcttccgcgg ccgcgccggc gaggcggccg cgttcacccg cgaggcggcc cggcacgtgc
      901 ccgaggagat ggcccccatg cgcgccgtgc tcgagggcat cgagctgggc accacgatgt
      961 tcggggtggc cgagccggac atggtccggc ggctcggcga gtaccgcgac cggccggtgg
     1021 ggcccgggcc gggcgacaag atgatcgcgg ccatgaccgc gtgggagtgg gcgctcaccg
     1081 gcggcggcgc cgacgagtgc gtggagctgg cccggcaggc gctgaccgac gacagcgtgg
     1141 tggtggccga gaacggattc ctgaccgtgc cggcgatcac cgtgatggcc atggcggacc
     1201 tggacgaggc ggccgaacgc tgggatcgcc tggtggccca gacgcaggcc tccggctcgc
     1261 acttcgccgt ctcgtgcgcg cacctgtgga gcgggatcac ccagacccgc cggggggagc
     1321 tggccgaggc cgaggcgctg ctccgcaccg gccgggacga gctgtccatg tgggggccgc
     1381 tgccggtgga cggggcctac ttctccggca cgatccccct ggtgctgctg gagcggggcg
     1441 acgtggaggc ggcacggcgc gagctgggcg agcgtccatc gcgcatctac gaccaccacg
     1501 acgccggcca cctctggctg cggtgcgaga ccgagatcct gctggccgag ggcaggaacg
     1561 aggaggcgct cgagcgctcg gaggagttcg gcgaggtggc cgggcggctg gtcaaccccg
     1621 cctgggtgcc gtggcgctcg ctccaggcga tcgccctgga ccggctcggc cgcaccggcg
     1681 aagcggtcac ccgggccgag gaggagctgg agcacgcgcg ccgctggggc gcgccgggca
     1741 cggtcggccg cagcctgcgg gtgctgggca ccctgcgccg cgcggagggg ctggagctgc
     1801 tggaccaggc cgtggccctg ctcgagcgct cacccgcccg cctggagctg gcgaaggcgc
     1861 tggcggccca gggcgccacc ctgcgccgcg agcgcaagcc caccgacgcc cgcgacccgc
     1921 tccggcgcgc gctggagctg gccgagctgt gcgacgccgg cggcctggcg gccgaggtgc
     1981 gcacggagct gtacgccacc ggcagccggc cgcggtcgaa cgccctgacc gggcccgagg
     2041 cgctgaccgc gagcgagaaa cgcgtggccg agctggccgc cgagggcctg accaaccgcg
     2101 acatcgccca gacgctgttc gtcacgccca agacggtgga ggtccacctg agcgcgacct
     2161 accgcaagct gggcatccgc tcccggcgcg agctggccgg gtcgttcgcg gcagcctgag
     2221 ccgggcgggt gtagcgtggg ctgacccccc gcgtgagcac ctccacctcc accatcctgg
     2281 acctctccgt ccacgagacg gaggggacgc tgctggtgcg cctggacggc gagttcgacc
     2341 tggcgggcgt cggcatggtc gagaacgcgc tggaccggat gtcccggcct ccgccgctgg
     2401 cgcgggtggt cttcgacctg cgtgagctgg agttcctgga cctggccggc ctccagacca
     2461 tcctgcgtgc cgacgcccgc gcgcgggccg gcggctacga ggtcgtggtg gtgcgtccgc
     2521 acggcacggc caaccgcgtg ttcaccctca cccgcgcggg cgagacgctg aacatggccg
     2581 accggccgcc gtactaaggg gcgcgctcag cccagcggag ccggcgggat ccgggtcgag
     2641 ccgtgcacgt cggggagcag catcccggcg tcgtcgacga agcagaacga ccactcctcg
     2701 cccggctcca gcgaccggat cagcggatgg cccgactcct cgaagtgctt ggaggcgtgc
     2761 tggttgggcg agctgtcaca gcagcccacg tgcccgcatc cgaggcagat gcgcaggtgc
     2821 agccaccgtc cgccggtggc caggcagtcg gtacagccct cgaccgactc cggcagctcg
     2881 gtgatcgcga cggtgtccag gtgggtgcag gtggccatgc cccacggtct acaccatcag
     2941 cgcgcggcca gcggccggag ccccgccgtg ggcgctgcgg ccagggcgtc ctccagtgcc
     3001 cccaggccgg ccagcagccc gtcaaccgac tcggccgccg cgtcggtggg ctccccgtcg
     3061 gcgaagtcgc gcgaggtcag gtagccggcc acgggcacca cgtgggcgcc gaagaacgcc
     3121 agcacgtcgc gcaggtggcg ctcggcgccc aggaagtggt gcgggctggc gcccatggcc
     3181 aggagcgcga ccggcttgcc ctccagcgtg tcgatcggca acaggtccaa caggttcttc
     3241 agcgccccgg tcagcgaccc gcggtagacc ggcgtggcca gcagcacggc cccggccgcg
     3301 tccacccgcc ccaggacctc ggcggtggcc gcgtccgggt cggtcccgtc ggcgaagggc
     3361 agccggacct cggccaggtc
//
LOCUS       DASUPW010000325         3797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_494558, whole genome shotgun sequence.
ACCESSION   DASUPW010000325 DASUPW010000000
VERSION     DASUPW010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494558"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VFY44_07265"
     CDS             <1..431
                     /locus_tag="VFY44_07265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5910276.1"
                     /translation="ALEEEVRRRGLPNVVLLPAGDKAAAARLAAASDACLTVFKDVPV
                     LATNSPNKLFDTFAAGRAAIVNTGGWQRQLVEEGDAGVYVRPGDGEDLAAKVLALRDD
                     PELTATMGANARRLAEARFDRRDLAEQLRVVLEEAASHSR"
     gene            445..1362
                     /locus_tag="VFY44_07270"
     CDS             445..1362
                     /locus_tag="VFY44_07270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5910277.1"
                     /translation="MVNTNGREYLRSCLAAIERVHPPGLERELLVLDNHSDDGSAAMV
                     REQWPQARLIELDRRTGKAENDSTLLRAATGRHCLLLNEDTELQDGSVAALVAALDDD
                     RGAAAAGAQLLTADGTPTACAWRLPGLGTALATAVFAHRNLVVQSRGRETRPVGWVQS
                     SAMLVRRTAAEQVDYLDPDFFVYSDETDFCKRLHDAGWRVLFVPGARAVHHDQLNTDL
                     AASRRRIVEFHRNRDLYLRKHHGPLTRLVVRALGAWFYLATAAAAVVVPGHDPRRYLL
                     NARQELAPGSGEGIREAAAEYNSALHGTP"
     gene            1349..2872
                     /locus_tag="VFY44_07275"
     CDS             1349..2872
                     /locus_tag="VFY44_07275"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX5910278.1"
                     /translation="MEHPDLAQLAGVVAAAGVILMLAGRGRLMVLGGLGLLGAAEAGL
                     LASRSDTASFDRLASASGAVLVVFGLLVMAAAAAVLVRRPGWLPVVMILAAPLRPPIA
                     FESGGGFPLSIATGGQLGRLLPLYFVLAVAAVALVWRAFDPAQSAGAVRALPRVVALP
                     AAAFAAFSCLSVVWADQLGPAVELLSYFTIPFVVLLAVLARSPFPDTAPRAMAIAAIA
                     LGTVFAAIGLYQAATRELFFYAPNLAVSNANSDFFRVTSLFGDPSLYGRHLVLAMTVL
                     LVCLALIRIDLRLGIGLLVVLWLGLFFSYSQSSMVALVCVTLAIAFATGGPPVRRVVA
                     GGLALVLVLGLGFLASIEIRGDSLRRETSDRSTRITETTRVVEDAPLIGVGTGGQAQA
                     SRRLSGKEKPTPNFVSHFTPLTVAAELGVVGLALYGWLLVGGARAILAVGRLDRGLGI
                     ALGAALLALFTHSLFYPGFLEDPLTWVVLAVAAGQLTWSRRDDGVHRAARDAAPASA"
     gene            2944..>3797
                     /locus_tag="VFY44_07280"
     CDS             2944..>3797
                     /locus_tag="VFY44_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910279.1"
                     /translation="MFALLGVLLGIVAVTLPELGSDPWPFRPGAVDPQGILSPLVRAA
                     DSEWDVGIARTTCFLAALLIAAVGVVGLRRRDWPAWAGVGVAVLVALALMLPSTLLQM
                     GLRQSTEPWFFTNDSTYQIEIGGDKLLDGENPYGADYRFSGMERFYPFDGRALERVRD
                     REVALEHFAYFPGTAIAGAVWRVLPEPFDDYRMLVLLFTLGGLAAALAFRAPLEWRLV
                     VGAVIVANPIAVRSAWFGQNDAPSITLMILAFALATRGRARWAAAALAGAVLLKQFAV
                     VAIPFLAV"
BASE COUNT          390 a         1412 c         1414 g          581 t
ORIGIN      
        1 ccgcgctgga ggaggaggtg cgccgtcgcg ggctgcccaa cgtggtgctg ctgcccgcgg
       61 gcgacaaggc cgccgccgcc cggctggccg cggcctcgga cgcctgcctg accgtgttca
      121 aggacgtgcc cgtgctggcc accaactcgc cgaacaagct gttcgacacg ttcgccgcgg
      181 gccgcgctgc gatcgtgaac acgggcgggt ggcagcgcca gctcgtggag gagggcgacg
      241 ccggggtgta cgtgcgcccc ggcgacggcg aggacctggc cgccaaggtg ctggccctgc
      301 gcgacgaccc ggagctgacg gcgacgatgg gcgccaacgc gcgccgcctg gccgaggccc
      361 ggttcgaccg ccgcgacctg gccgagcagc tgcgcgtcgt cctggaagag gcggcctcac
      421 attcccgctg acgtctccta ctgcgtggtc aacaccaacg gccgcgagta cctgcgctcg
      481 tgcctggcgg ccatcgagcg ggtgcaccct ccggggctgg agcgcgagct gctggtgctg
      541 gacaaccact cggacgacgg gtcggccgcc atggtgcggg agcagtggcc gcaggcgcgg
      601 ctgatcgagc tggaccggcg cacgggcaag gccgagaacg actccaccct gctgcgcgcc
      661 gccaccggcc gccactgcct gctgctgaac gaggacaccg agctccagga cggctcggtg
      721 gccgcgctgg tggcggcgct ggacgacgac cgcggcgccg ccgccgcggg cgcgcagctc
      781 ctgaccgccg acggcacgcc caccgcgtgc gcctggcgcc tgcccggcct cggcacggcc
      841 ctggccacgg cggtcttcgc ccaccgcaac ctggtggtgc agagccgcgg gcgggagaca
      901 cggccggtgg gctgggtgca gtccagcgcg atgctggtgc gccgcaccgc ggccgagcag
      961 gtggactacc tggacccgga cttcttcgtg tactcggacg agacggactt ctgcaagcgc
     1021 ctgcacgacg ccggctggcg cgtcctgttc gtccccggcg cccgggccgt gcaccacgac
     1081 cagctgaaca ccgacctggc cgcctcgcgg cggcggatcg tggagttcca ccggaaccgc
     1141 gacctctacc tgcgcaagca ccacggcccg ctcacgcgcc tggtcgtgcg ggcgctgggc
     1201 gcctggttct acctggccac cgccgccgcg gccgtggtcg tgccgggcca cgacccgcgc
     1261 cgctacctgc tgaacgcgcg ccaggagctg gctcccgggt cgggcgaggg gatacgcgag
     1321 gccgccgccg aatacaactc tgccctccat ggaacaccct gacctggccc aactcgcggg
     1381 agtcgtcgcc gccgccggcg tgatcctgat gctggccggc cgcggccggc tgatggtgct
     1441 gggcggcctg ggcctgctgg gcgccgccga ggccgggctg ctggcctcgc gctcggacac
     1501 cgcctccttc gaccgcctgg cctcggcctc gggggccgtg ctggtggtct tcggcctgct
     1561 ggtcatggcc gccgcggccg ccgtgctggt gcgccgcccg ggctggctgc cggtggtcat
     1621 gatcctggcc gcgccgctgc ggcccccgat cgcgttcgag tccggcggcg ggttcccgct
     1681 gtcgatcgcc accggcgggc agctggggcg cctgctgccg ctgtacttcg tcctggccgt
     1741 ggccgccgtc gcgctggtgt ggcgcgcctt cgaccccgcc cagtccgcgg gcgcggtgcg
     1801 cgcccttccg cgcgtggtgg cgctgcccgc cgccgcgttc gccgccttct cgtgcctgtc
     1861 ggtggtctgg gccgaccagc tgggccccgc ggtcgagctg ctgtcgtact tcacgatccc
     1921 gttcgtggtg ctgctggcgg tgctggcccg gtcgccgttc cccgacacgg ctccgcgcgc
     1981 catggccatc gcggccatcg cgctgggcac cgtgttcgcc gccatcggcc tctaccaggc
     2041 ggccacgcgc gagctgttct tctacgcccc caacctggcg gtgtccaacg ccaacagcga
     2101 cttcttccgc gtgacctcgt tgttcggcga ccccagcctg tacggccgcc acctggtgct
     2161 ggccatgacg gtcctgctgg tctgcctggc gctgatccgg atcgacctgc gcctgggcat
     2221 cgggctgctg gtggtgctgt ggctgggcct gttcttctcc tactcgcagt cgagcatggt
     2281 ggccctggtc tgcgtgacgc tggccatcgc cttcgccacc ggcggcccgc cggtcaggcg
     2341 ggtggtggcg ggaggcctgg cgctggtgct ggtgctgggc ctcggcttcc tggcctcgat
     2401 cgagatccgc ggggactcgc tgcggcgcga gaccagcgac cgctcgaccc gcatcaccga
     2461 gaccacgcgg gtggtcgagg acgcccccct gatcggcgtc ggcaccggcg ggcaggccca
     2521 ggccagcagg cggctgtcgg gcaaggagaa gcccacgccc aacttcgtct cgcacttcac
     2581 cccgctgacc gtggccgccg agctgggcgt ggtcgggctg gcgctgtacg gctggctgct
     2641 ggtgggcggg gcgcgcgcga tcctggcggt gggccggctg gaccggggcc tgggcatcgc
     2701 cctgggcgcc gcgctgctgg cgctgttcac ccactcgctc ttctatccgg gcttcctgga
     2761 ggacccgctg acgtgggtgg tgctggcggt ggccgccggg cagctgacct ggtcgcggcg
     2821 cgacgacggg gtgcaccggg ccgcgcgcga cgcggcaccg gcctcggcat gagcggccgc
     2881 cggggaggag cggaggcggg gcgtcggccc gccgagcacc gcaccaggtt gtcgcgggag
     2941 gggatgttcg ccctcctggg cgtgctgctg ggcatcgtgg ccgtgacgct gcccgagctg
     3001 ggctcggacc cgtggccgtt ccggcccggc gccgtggacc cccagggcat cctgtcgccg
     3061 ctggtgcgcg ccgccgacag cgagtgggac gtgggcatcg cccggaccac gtgcttcctg
     3121 gccgcgctgc tgatcgccgc ggtcggcgtg gtggggctgc gccgccgcga ctggcccgcg
     3181 tgggccgggg tgggcgtggc ggtgctggtg gcgctggcgc tgatgctgcc ctcgaccctg
     3241 ctccagatgg gcctgcgcca gtccaccgag ccgtggttct tcaccaacga ctccacctac
     3301 cagatcgaga tcggcggaga caagctcctg gacggcgaga acccgtacgg cgccgactac
     3361 cgcttctcgg gcatggagcg cttctacccc ttcgacgggc gcgcgctcga gcgtgtgcgc
     3421 gaccgcgagg tggcgctcga gcacttcgcc tacttccccg ggaccgccat cgcgggcgcc
     3481 gtctggcggg tgctgcccga gcccttcgac gactaccgga tgctggtgct gctgttcacg
     3541 ctcggcggcc tggctgccgc gcttgccttc cgggccccgc tggagtggcg cctggtcgtc
     3601 ggggcggtga tcgtggccaa cccgatcgcc gtgcgctcgg cctggttcgg ccagaacgac
     3661 gccccctcga tcacgctgat gatcctggcc ttcgcgctgg ccacccgcgg gcgcgcgcgc
     3721 tgggccgccg ccgcgctggc cggcgccgtg ctgctgaagc agttcgccgt tgtggccatc
     3781 cccttcctgg ccgtgat
//
LOCUS       DASUPW010000326         7942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_770707, whole genome shotgun sequence.
ACCESSION   DASUPW010000326 DASUPW010000000
VERSION     DASUPW010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7942
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770707"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VFY44_07285"
     CDS             complement(<1..426)
                     /locus_tag="VFY44_07285"
                     /inference="COORDINATES: protein motif:HMM:NF014762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX5910280.1"
                     /translation="MAHDHATVAEEEYLQALFWLQEARLPMTAANVARAMQLSPPTVH
                     EMIGRLERDGYVSRKPDKTLYFTDTGREHASAIVKRHRLVERFLTDVLGIPWDDVHEE
                     AERLEHAMSPVLEERMLAAIGDARTCPHGHPIGEVEREPG"
     gene            complement(437..1156)
                     /locus_tag="VFY44_07290"
     CDS             complement(437..1156)
                     /locus_tag="VFY44_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910281.1"
                     /translation="MAPRLLEFADELRSEGMAVGTSELLDGFRALEAVSWNDRTQFRE
                     ALSATLAKSQEDRRVLELVFDRFFFRAAEREAVEQGVREADAHAASDGGDRMDLDELR
                     QAIRDALQSEDSGEGQMRDLARLAIAAFGRQGEGSGVIGVDVQRIRRTLDLKGEQPNA
                     QQPDPEAVPRDRLREFERHVRRELERALIERTESLPPSRPLNELDRALPTGAAQDLAD
                     VHRVVAQLRRRLAAEAAAEAG"
     gene            complement(1257..2147)
                     /locus_tag="VFY44_07295"
     CDS             complement(1257..2147)
                     /locus_tag="VFY44_07295"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5910282.1"
                     /translation="MSELDSIEATRAALGEAGYLADDSASLVSYLAAQLGKPVLVEGP
                     AGVGKTELAKALSRATGRKLVRLQCYEGLDEAKALYEWNYRKQLLRIQVEASDAGWQE
                     VKDDIFGEEFLLPRPLLSAISATEPVVLLIDEIDKTDQEFEAMLLEVLSDFQVSIPEL
                     GVVEARTRPIVLLTSNNSRELTEALKRRCLYLWLDYPDVERELEIVRMHTPELSEEMA
                     RKLVEVVQMVRNLDLKKPPSIAESIDWARALLLLGAQDIDSQTLKNTMSIIVKHRTDL
                     DLVAERVGVKLTDGLVGPRG"
     gene            2173..2403
                     /locus_tag="VFY44_07300"
     CDS             2173..2403
                     /locus_tag="VFY44_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910283.1"
                     /translation="MLAALTLLAQGGLPGLGQDGPEDLQRLLKMFYVLFTGGAIIAIL
                     GYAIPSKMLQILGIALVFLSTAVFMAAVSGYG"
     gene            complement(2400..2894)
                     /locus_tag="VFY44_07305"
     CDS             complement(2400..2894)
                     /locus_tag="VFY44_07305"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5910284.1"
                     /translation="MDGLTTEHLRLRPLEAADAEPLFEVYSHPLVEAWIGPHSLQRVT
                     DEIAFQRAAQAQRGFSVWAVETRDGGRFLGDCGLQPLEHRGPEMELGYDLHPDVWGQG
                     LATEAARAVVDAAFGPLGLRRVVAVVKPAHAASRRVLEKAGLREAGRRGAYGELMVLY
                     EAVA"
     gene            2932..3585
                     /locus_tag="VFY44_07310"
     CDS             2932..3585
                     /locus_tag="VFY44_07310"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX5910285.1"
                     /translation="MAEDDPTTPPEPRQRRARKRSAADGIRAKEFPPAMRGYDRTAVD
                     AWREEVAELVLQLEDQAPRDAAVRRALDEVGKETSAILQRAHEAADDIASRSRSQAEG
                     RLQRAEREAEITVSEAEARVLELNEDYKRIWDERARLLDEMRQLADDVLGVADDAADR
                     IAPPREPDPITEENETVAVAETETETEAETEGAGAEDPTQVVEPQTPAGAQAPPKPS"
     gene            complement(3582..4217)
                     /locus_tag="VFY44_07315"
     CDS             complement(3582..4217)
                     /locus_tag="VFY44_07315"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEX5910286.1"
                     /translation="MSIRTDAVDAYFGRLRPERAPVMAEMEALADRDDVPIVSWETGR
                     LLSALAAAGDGRILEVGTAIGYSALHMAEALQAGSIVTLERDPGRIAQARDFLDRAGV
                     GDRVEIVEGDARETIPTLDGPFDFLFLDATKGEYRDYLELAEPLLSPRALLAVDNVLM
                     SGKVADPGAEGHWSEEQLETQRAFNAELVGGSGWRASVLPVGDGVLVGSRP"
     gene            4265..5359
                     /locus_tag="VFY44_07320"
     CDS             4265..5359
                     /locus_tag="VFY44_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase 2"
                     /protein_id="HEX5910287.1"
                     /translation="MSEVAEVQSGLEGVVAFATEIAEPDKEGGSLRYRGVDIEDLVGQ
                     VPYEKVWGLLVDGSFDVGLPPAVPHALTVRTGDPRTDLQASLAMLGPEWGLEPVIDID
                     DETAREQLAQVSVMGLSFVAQSARGIGKPPVPQTEVDKAQSIPERFLIRWRGEANPDH
                     VKAIDAYWISAAEHGMNASTFTARVITSTGADVAAALSGAVGALSGPLHGGAPSRVLS
                     MLDGVEEKGDAEAWVKEALDRGDRLMGFGHRVYRAEDPRARVLRETAREIGAPRLEAA
                     EALEEAALAELKARKPDRVLATNVEFWSAVVLDFADVPSNLFTPLFASARVGGWSAHI
                     LEQKREARLIRPTAKYVGEDPRPVADVGDV"
     gene            complement(5356..6216)
                     /locus_tag="VFY44_07325"
     CDS             complement(5356..6216)
                     /locus_tag="VFY44_07325"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5910288.1"
                     /translation="MLDVTRLRVIEAVARHGSVTRAAKELNYSQPSVTHHLGRLEAET
                     GAQLLQRVGRGIRVTPAGRLLADRAAEILGRIDAADSELAAHVGLTAGRVRLGAFASA
                     SAQLVPGAVAALAATHPGLQVSVTDMHPPDAIEMLRTGEIEVAIIFRYDEDAPEPAGV
                     RLHHLLDDPVHLLSTRPEDGLESLRDATWIAGCERCSSHLLSICAEAGFEPRIGSRSD
                     DMVVMAALVAAGMGVATQTGLALRAHQLEGVVTTELPGSQRHIYAATYGEPPDPPPTA
                     ALLEALAQAA"
     gene            6291..6800
                     /locus_tag="VFY44_07330"
     CDS             6291..6800
                     /locus_tag="VFY44_07330"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEX5910289.1"
                     /translation="MIETATPPAELTRLVDGVRGAVDAHAGWSETASLVADELRLHLP
                     GPGVLTAEQRAGSPESYQGHTLHVEPDGSFSIVALVWRPGQVTRIHDHTTWCVFGVVQ
                     GVEHEDLFDAGLNLIGSSDNHVGDVSGFAPPGDIHRVHNTAGTTAISIHIYGTDVTRL
                     GSSARRYYD"
     gene            6812..7444
                     /locus_tag="VFY44_07335"
     CDS             6812..7444
                     /locus_tag="VFY44_07335"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX5910290.1"
                     /translation="MSLVYVHFFSVSLDGFGTGEGQTRDLHFGHAGERLHTWMFKTRT
                     WESGGSGGIDDLLIQRHDAGIGAEIMGAGKFGHPGWEDDPDWKGAWGPNPPFHTPVFV
                     LTHHTRPSIEMEGGTTFHFLDASPAEALEKAREAAGGLGVRIGGGPSVIRDFLAARLI
                     DHAHIVVVPIVLGRGVRLWDGLEGLEEKYDVEAVSSPTGVTHLTFSRPGI"
     gene            7464..7814
                     /locus_tag="VFY44_07340"
     CDS             7464..7814
                     /locus_tag="VFY44_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5910291.1"
                     /translation="MAIERFDLADMHVSPTAALFEGGDRGAGVSVFVTRTPPGSWVDL
                     HVHPYPETFVLLEGHGRWTVGEEPVELGPDQMVVVPPETPHGFRNTGDRPLLVVSVHE
                     RGTLEQTWLDREPL"
BASE COUNT         1087 a         2925 c         2841 g         1089 t
ORIGIN      
        1 gcccggctcg cgctcgacct cgccgatcgg gtggccgtgc ggacaggtgc gcgcgtcgcc
       61 gatcgcggcc agcatgcgct cctcgagcac cggcgacatc gcgtgctcga gccgctccgc
      121 ctcctcgtgc acgtcgtccc acgggatgcc gaggacatcg gtcaggaagc gctcgaccag
      181 ccggtggcgc ttgacgatcg ccgaggcgtg ctcgcgcccc gtgtcggtga agtagagggt
      241 cttgtcgggc ttgcgcgaga cgtagccgtc gcgctccagg cgcccgatca tctcgtggac
      301 ggtggggggc gagagctgca tcgcccgcgc gacgttggcg gcggtcatgg ggagccgcgc
      361 ctcctgcagc cagaagagcg cctggaggta ctcctcctcg gcgacggtcg cgtgatcgtg
      421 ggccatgacg gtgatgctat ccggcctcgg cagccgcctc ggcggccagg cgccggcgca
      481 gctgggcgac cacccggtgc acgtcggcca ggtcctgggc ggccccggtg ggcagcgcgc
      541 ggtccagctc gttcagcggg cgcgagggcg gcagcgactc ggtgcgctcg atcagcgcgc
      601 gctccagctc gcggcggacg tggcgctcga actcgcgcag gcggtcgcgc ggcacggcct
      661 cggggtcggg ctgctgggca ttgggctgct cgcccttgag gtcgagcgtg cggcggatgc
      721 gctggacgtc cacgccgatc acgcccgagc cctctccctg acgcccgaag gcggcgatgg
      781 ccaggcgggc caggtcgcgc atctggccct cgcccgagtc ctcggactgg agcgcgtcgc
      841 ggatggcctg gcgcagctcg tccaggtcca tccggtcgcc gccgtccgag gcggcgtggg
      901 cgtcggcctc gcgcacgccc tgctccacgg cctcgcgctc ggcggcgcgg aagaagaagc
      961 ggtcgaacac cagctccagc acccgccggt cctcctggct cttggccagc gtggccgaca
     1021 gggcctcgcg gaactgggtg cggtcgttcc agctgaccgc ctccagcgcg cggaagccgt
     1081 ccaggagctc ggaggtgccc acggccatgc cctcgctgcg cagctcgtcg gcgaactcca
     1141 gcaggcgcgg agccatgccg ggcgggccgg agggctcgcc cgtcccgccg tacagcttgg
     1201 ggttagcccg gggaggaccg gagcccgatc ggtgatcggg cgaggaccgg accccgttat
     1261 ccgcggggcc cgacgaggcc atcggtcagc ttcactccca cccgctcggc caccaggtcg
     1321 aggtcggtgc ggtgcttgac gatgatgctc atggtgttct tgagcgtctg ggagtcgatg
     1381 tcctgggcgc ccagcagcag cagcgcccgc gcccagtcga tcgactcggc gatcgagggc
     1441 ggcttcttga ggtccaggtt gcgcaccatc tgcacgacct cgacgagctt gcgcgccatc
     1501 tcctccgaca gctccggggt gtgcatgcgc acgatctcga gctcgcgctc gacgtccggg
     1561 tagtccagcc acaggtacag gcagcggcgc ttgagcgcct cggtcagctc acgcgagttg
     1621 ttcgaggtca gcagcacgat cggccgggtc ctggcctcga cgacccccag ctccgggatc
     1681 gacacctgga agtccgacag cacctccagc agcatcgcct cgaactcctg gtcggtcttg
     1741 tcgatctcgt cgatcagcag caccaccggc tcggtggcgg agatggccga gagcagcggg
     1801 cgcggcagca ggaactcctc gccgaagatg tcgtccttga cctcctgcca gccggcgtcg
     1861 gaggcctcga cctggatccg cagcagctgc ttgcggtagt tccactcgta cagcgccttg
     1921 gcctcgtcca ggccctcgta gcactggagc cgaaccagct tgcggccggt ggcgcgcgac
     1981 agcgccttgg ccagctcggt cttgcccacg ccggccggtc cctcgaccag caccggcttg
     2041 cccagctggg cggccaggta cgagaccagg gaggccgagt cgtccgccag gtagcccgcc
     2101 tcgcccagcg cggcgcgcgt ggcctcgatg gagtcgagct cgctcacggc cgacaggata
     2161 cgctgcgccg ttgtgctcgc cgccctgacc ctcctcgcgc aaggaggcct tcccggtctg
     2221 ggccaggacg gaccggagga tctccagcgc ctgctcaaga tgttctacgt gctgttcacg
     2281 ggcggggcga tcatcgccat cctgggttac gcgatcccgt cgaagatgct gcagatcctg
     2341 ggaatcgcgc tggtcttcct gtccaccgcg gtgttcatgg ccgcggtcag cggatacggc
     2401 taggcgacgg cctcgtagag caccatcagc tcgccgtagg caccccggcg gccggcttcg
     2461 cgcaggccgg ccttctccag cacccgccgt gacgccgcgt gtgccggttt gacgaccgcc
     2521 accacccgcc tcaggccgag cgggccgaac gcggcgtcca ccacggcacg ggccgcctcc
     2581 gtggccaggc cctggcccca cacgtcgggg tgcaggtcgt agcccagctc catctcgggc
     2641 cccctgtgct ccagcggctg gagcccgcag tcgcccagga agcggccccc gtcgcgggtc
     2701 tcgactgccc acaccgagaa gccgcgctgg gcctgcgccg cgcgctggaa ggcgatctcg
     2761 tcggtcaccc gctggagtga gtgcgggccg atccaggcct cgaccagggg gtgggagtac
     2821 acctcgaaca ggggctccgc atcggcggcc tccagcggcc gcagccgcag gtgctcggtg
     2881 gtcaggccgt ccatgtgccc agtgtccccg acgttcgcaa tactcgggcc catggctgag
     2941 gacgatccca ccacaccccc cgagccccgt cagcggcgtg cccgcaagcg cagcgcggcc
     3001 gacggcatcc gcgcgaagga gttcccgccc gccatgcgcg gctacgaccg cacggcggtc
     3061 gacgcctggc gcgaggaggt ggccgagctg gtgctccagc tcgaggacca ggctccgcgc
     3121 gacgccgcgg tccggcgcgc gctcgacgag gtcggcaagg agacgtcggc catcctccag
     3181 cgcgcccacg aggcggccga cgacatcgcc tcgcggtcgc gctcgcaggc ggagggccgg
     3241 ctgcaacgcg ccgagcgcga ggccgagatc accgtgtccg aggccgaggc gcgcgtcctc
     3301 gagctcaacg aggactacaa gcgcatctgg gacgagcggg cgcggctgct ggacgagatg
     3361 cgccagctgg ccgacgacgt gctcggcgtg gccgacgacg ccgccgaccg gatcgccccg
     3421 ccgcgcgagc cggaccccat caccgaggag aacgagaccg tcgcggtcgc ggagacggag
     3481 acggagacgg aggcggagac cgagggggcc ggggccgagg acccgaccca ggtcgtcgag
     3541 ccgcagacgc ccgccggggc gcaggcgccc cccaagccct cctagggccg gctgcccacc
     3601 agcacgccgt cgcccacggg cagcaccgac gcgcgccagc cgctgccgcc caccagctcg
     3661 gcgttgaagg cccgctgggt ctcgagctgc tcctccgacc agtggccctc ggcgccgggg
     3721 tcggccacct tgcccgacat cagcacgttg tccacggcca gcagcgcccg gggggacagc
     3781 agcggctcgg ccagctccag gtagtcgcgg tactcgccct tggtggcgtc caggaagagg
     3841 aagtcgaacg ggccatccag ggtagggatc gtctcccgcg cgtcgccctc cacgatctcg
     3901 acccggtcgc ccacgccggc gcggtccagg aagtcccgtg cctgggcgat ccggcccgga
     3961 tcgcgctcca gggtcacgat cgaccccgcc tgcagggcct cggccatgtg cagcgccgag
     4021 tagccgatgg cggtgcccac ctccaggatc cggccgtccc cggcggccgc cagcgcggac
     4081 agcagccggc ccgtctccca cgacacgatc gggacgtcgt cgcggtcggc cagcgcctcc
     4141 atctcggcca tgaccggggc gcgctcgggg cgcagccgcc cgaagtaggc gtccaccgca
     4201 tcggtcctga tgctcatgcg cggctagtct ccgttccgat cttgatccag ttcgaggaga
     4261 gcatatgtcc gaggtagccg aggttcagtc cggactcgag ggcgtcgtcg cgttcgcgac
     4321 ggagatcgcc gagccggaca aggaaggcgg ctcgctgcgc taccgcggcg tggacatcga
     4381 ggacctggtg ggccaggtgc cctacgagaa ggtctggggc ctgctggtgg acggctcctt
     4441 cgacgtcggc ctgcccccgg ccgtcccgca cgcgctgacc gtgcgcaccg gcgacccgcg
     4501 caccgaccta caggcctcgc tggccatgct gggcccggag tggggcctcg agccggtcat
     4561 cgacatcgac gacgagaccg cccgtgagca gctggcgcag gtctcggtca tggggctctc
     4621 gttcgtggcc cagtccgccc gcgggatcgg caagccgccc gtgccgcaga ccgaggtcga
     4681 caaggcccag tcgatccccg agcggttcct gatccgctgg cgcggtgagg ccaacccgga
     4741 ccacgtcaag gccatcgacg cctactggat ctcggccgcc gagcacggca tgaacgcctc
     4801 gaccttcacc gcccgcgtga tcacctcgac cggcgccgac gtggccgccg ccctgtccgg
     4861 cgccgtcggc gccctgtccg gcccgctgca cggcggcgcg ccctcgcgcg tgctgtccat
     4921 gctcgacggc gtcgaggaga agggcgacgc cgaggcctgg gtcaaggagg cgctggaccg
     4981 tggcgaccgc ctgatgggct tcggccaccg cgtctaccgc gccgaggacc cgcgcgcccg
     5041 cgtgctgcgc gagaccgcgc gtgagatcgg cgccccgcgc ctggaggcgg ccgaggcgct
     5101 ggaggaggcc gccctggccg agctcaaggc ccgcaagccc gaccgcgtcc tggccaccaa
     5161 cgtcgagttc tggtcggcgg tggtgctcga cttcgccgac gtgccgtcga acctgttcac
     5221 gccgctgttc gcctcggcgc gcgtgggcgg ctggtcggcg cacatcctgg agcagaagcg
     5281 cgaggcgcgg ctgatccggc cgaccgccaa gtacgtgggc gaggacccgc gcccggtggc
     5341 cgacgtcggc gacgtctagg ccgcctgcgc cagcgcttcg agcagggccg cggtcggcgg
     5401 cgggtcgggt ggctcgccgt acgtcgccgc gtagatgtgg cgttgcgagc cgggcagctc
     5461 ggtggtcacg actccctcga gctggtgggc gcgtagcgcc aggccggtct gggtggccac
     5521 gcccatgccg gcggccacca gcgcggccat gacgaccatg tcgtccgagc ggctgccgat
     5581 ccggggctcg aacccggcct cggcacagat cgagagcagg tggctgctgc agcgctcgca
     5641 gcccgcgatc caggtcgcgt cgcgcagcga ctccagcccg tcctcggggc gggtcgagag
     5701 caggtggacc gggtcgtcga gcaggtggtg caggcgcacc ccggccggct cgggcgcgtc
     5761 ctcgtcgtag cggaagatga tcgcgacctc gatctcgccc gtgcgcagca tctcgatcgc
     5821 gtcgggtggg tgcatgtcgg tcacgctgac ctgcagcccg gggtgggtgg cggccagcgc
     5881 cgcgaccgcg ccgggaacca actgagcgct cgcggaggcg aacgcgccca ggcgcacgcg
     5941 gccggccgtc agccccacgt gggcggccag ctcggagtcg gcggcgtcga tgcgaccgag
     6001 gatctcggcc gcgcggtcgg ccagcagccg gccggcgggg gtcacccgga tgccccgccc
     6061 tacccgctgc agcagctgcg cgccggtctc ggcctcgagc cgccccaggt ggtgggtcac
     6121 ggagggctgt gagtagttca gctccttcgc cgccctggtc accgatccgt ggcgcgcgac
     6181 ggcctcgatc acccgcagcc gggtcacgtc gagcatgcat caactctatc tatggatcac
     6241 gcagaaagtt ggcattggac gtatgagtcg ccgggcggga ggctgcccgc atgatcgaga
     6301 ccgcgacacc ccccgccgaa ctcaccaggc tcgtggacgg cgtccgcggc gccgtggacg
     6361 cccacgccgg ctggtctgag accgcctcgc tcgtcgccga cgagctgcgc ctgcatctgc
     6421 ccgggcccgg cgtgctgacc gccgagcagc gcgcgggctc gcccgagagc taccagggcc
     6481 acaccctcca cgtcgagccc gacggctcgt tctcgatcgt cgcgctggtg tggcggccgg
     6541 gccaggtcac ccggatccac gaccacacca cgtggtgcgt cttcggcgtg gtccaggggg
     6601 tcgagcacga ggacctgttc gacgccggcc tcaacctcat cggcagcagc gacaaccacg
     6661 tgggcgacgt cagcgggttc gcgccgcccg gcgacatcca ccgggtgcac aacacggcag
     6721 gcaccacggc gatctcgatc cacatctacg gcaccgacgt cacccgcctg ggctcgagcg
     6781 cccggcgcta ctacgactga aaggaccacc catgtctctc gtctacgtcc acttcttctc
     6841 cgtctcgctc gacggcttcg gcaccgggga gggccagacc cgcgacctcc acttcggcca
     6901 cgccggggag cgcctgcaca cgtggatgtt caagacccgg acctgggagt ccggcggaag
     6961 cggcggcatc gacgacctcc tgatccagcg ccacgacgcc gggatcgggg ccgagatcat
     7021 gggcgcgggc aagttcggcc accccgggtg ggaggacgac ccagactgga agggcgcgtg
     7081 gggtcccaac ccgccgttcc acaccccggt cttcgtcctg acccaccaca cccgtccctc
     7141 gatcgagatg gaagggggca ccacgttcca cttcctggac gcctcgccgg ccgaggcgct
     7201 ggagaaggcc cgcgaggccg cgggcggcct gggcgtccgc atcggcggag gcccgagcgt
     7261 gatccgcgac ttcctggccg cccggctcat cgaccacgcc cacatcgtgg tggtcccgat
     7321 cgtcctcggc cgcggggtgc ggctgtggga cgggctcgag ggcctcgagg agaagtacga
     7381 cgtcgaggcc gtctcctcgc ccaccggggt cacgcacctg acgttctcgc ggccgggcat
     7441 ctgaggcgtt ctaggctggc ggtgtggcga tcgagcgctt cgacctggcg gacatgcacg
     7501 tgagccccac cgccgcgctg ttcgagggcg gcgaccgggg cgcgggggtc tcggtcttcg
     7561 tcacgcgcac cccgccgggc agctgggtcg acctgcacgt gcacccgtac cccgagacgt
     7621 tcgtgctgct ggagggccac ggccgctgga ccgtcggcga ggagccggtc gagctgggcc
     7681 cggaccagat ggtggtcgtg ccgcccgaga ccccgcacgg cttccgcaac accggcgacc
     7741 ggccgctgct ggtggtctcg gtgcacgagc gcggcacgct cgagcagacc tggctggacc
     7801 gcgagccgct ctagcccgcg gcctgctcgc ggcgctcgcg ctcgacggtg cgggcgacct
     7861 cgacccgtga ggagacgctg agcttcacga acacgttgcg caggtgggac tccaccgtct
     7921 tgccgctcag gaacagctcg gc
//
LOCUS       DASUPW010000327         2905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1187089, whole genome shotgun sequence.
ACCESSION   DASUPW010000327 DASUPW010000000
VERSION     DASUPW010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187089"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VFY44_07345"
     CDS             <1..631
                     /locus_tag="VFY44_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910292.1"
                     /translation="FTYLPRRVRGRLAGEDVHQPASETALVAWSSMRGSVSLAAALAI
                     PLTIDGGAPFPERDLIVFLAFCVILATLVGQGLLFPAVIRAVDIEDDTRDQDEELNAR
                     LEIAFAATDRIDEIAGEDWVYEGTADRVRNLYEYRQRRFRSRVGDTDHDDDEDYEARS
                     DGYRRFMGEVLAAQRRRLRALRDEGEITDEVRRTIERDLDLEQARLDPS"
     gene            complement(636..1427)
                     /locus_tag="VFY44_07350"
     CDS             complement(636..1427)
                     /locus_tag="VFY44_07350"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX5910293.1"
                     /translation="MPGEQAFYVPEGDGYRATELTRGPWDPGSQHAGPPCALLGRELD
                     RAGALDRGRLSRATFEILGPVPIGPLTVTAEVVRPGRSVELVEGTMAAGGRPVIRARA
                     WRLRTVEPMDFEPATPEPARVAGPEEGREVPFFPTGADVGYHTAMEVRFVHGAFTEPG
                     PALAWMRMRVALVAGEEPEPLHRVLVAADSGNGVSSPLDYREWMFINADVSVTLRRHP
                     EGEWVGLDASTYPEADGVGMSDTRLHDQRGMIGRANQALFVGPRP"
     gene            1466..2068
                     /locus_tag="VFY44_07355"
     CDS             1466..2068
                     /locus_tag="VFY44_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910294.1"
                     /translation="MAKQRQMKMQMQAGALQAIPLLESRTDEQLRSESKHRVAAKALL
                     GARAAEKYDAKAARAYFNEALAGCHPQERPALRQMMQASMALAERRPDDLKAAVEKLG
                     QEAPSSRQLFLLRIMGLIAPPPGAGALTRVRGIAILVGAVLVLLAVGFGLAKLVTLPF
                     GGVGTLPAIAIGLLIVVAVLGVLALFGRRRQARAAAARAG"
     gene            complement(2072..2776)
                     /locus_tag="VFY44_07360"
     CDS             complement(2072..2776)
                     /locus_tag="VFY44_07360"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5910295.1"
                     /translation="MRRAFLFGLVGVLLATGCGGDDDGGASADNTAAPDTQAAGTAAR
                     DSDACRRVQAPKPRAEGTQRRPPAANLAPGKRYDVTFRTNCGPFTIEVDQKAAPATAA
                     SFVSLARKGFFDGTVFHRIVPGFVIQGGDPTGSGQGGPGYTTTDKPPADTVYERGMVA
                     MAKTGEEPAGTAGSQFFVVTGQAPDLAPEYATLGRVTKGLGVVERIGQLGDPSSGGAG
                     TPMEPVVIGRTKVSAY"
BASE COUNT          370 a         1099 c         1055 g          381 t
ORIGIN      
        1 cttcacctac ctcccgcgcc gcgtgcgcgg gcgcctggcc ggagaggacg tgcaccagcc
       61 ggccagcgag acggccctgg tcgcgtggtc gagcatgcgc ggctcggtgt ccctggccgc
      121 ggcgctggcc atcccgctga ccatcgacgg cggcgccccc ttccccgagc gcgacctgat
      181 cgtgttcctg gccttctgcg tgatcctggc caccctggtc ggccagggcc tgctgttccc
      241 ggccgtgatc cgcgcggtgg acatcgagga cgacacccgc gaccaggacg aggagctgaa
      301 cgcgcggctg gagatcgcgt tcgcggccac ggaccggatc gacgagatcg ccggcgagga
      361 ctgggtctac gagggcaccg ccgaccgcgt gcgcaacctg tacgagtacc gccagcggcg
      421 cttccggtcg cgggtcggcg acaccgacca cgacgacgac gaggactacg aggcccgctc
      481 cgacggctac cgccggttca tgggcgaggt gctggccgcg cagcgccggc gcctgcgcgc
      541 cctccgggac gagggcgaga tcaccgacga ggtgcgccgc acgatcgagc gcgacctgga
      601 cctggagcag gcccggctgg acccctccta gcgcctcagg gccggggccc gacgaacagc
      661 gcctggttgg cccgcccgat catcccgcgc tggtcgtgca ggcgggtgtc ggacatgccc
      721 acgccgtcgg cctcggggta ggtcgaggcg tccaggccga cccactcgcc ctcggggtgg
      781 cgccgcagcg tcaccgacac gtcggcgttg atgaacatcc attcgcggta gtccagcggc
      841 gagctgacgc cgttgcccga gtcggcggcc acgaggacgc ggtgcagcgg ctcaggctcc
      901 tcgcccgcca ccagcgccac ccgcatccgc atccaggcca gcgccgggcc gggctcggtg
      961 aaggcgccgt gcacgaaccg gacctccatg gccgtgtggt agccgacgtc ggcgccggtg
     1021 gggaagaacg ggacctccct cccctcctcc gggcccgcca cccgtgccgg ctcgggcgtg
     1081 gccggctcga agtccatcgg ctccacggtg cgcaggcgcc aggcacgtgc ccggatcacc
     1141 ggccgcccgc cggcggccat cgtgccctcg accagctcga cgctgcggcc cgggcggacc
     1201 acctcggcgg tcacggtcag gggcccgatg ggcaccgggc cgaggatctc gaacgtcgcg
     1261 cggctcaggc gccctcggtc cagggcgccc gcgcggtcga gctcgcggcc cagcagcgcg
     1321 cacgggggcc ccgcgtgctg gctgcccggg tcccacggcc cccgagtcag ctcggtcgcg
     1381 cggtagccgt cgccttcggg cacgtagaac gcctgctcac cgggcacgcc gtcgatccta
     1441 tggtggtctg ccaaactggc gacccgtggc caagcagagg cagatgaaga tgcagatgca
     1501 ggcgggcgcc ctccaggcga tccccctgct cgagagccgc accgacgagc agctccggag
     1561 cgagtccaag caccgcgtgg cggcgaaggc gctgctgggc gcccgtgccg cggagaagta
     1621 cgacgccaag gcggcccggg cctacttcaa cgaggcgctg gccggctgcc acccccagga
     1681 gcgccccgcg ctgaggcaga tgatgcaggc gtcgatggcc ctggccgagc gccgccccga
     1741 cgacctcaag gcggccgtcg agaagctggg ccaggaggct ccctccagcc gccagctgtt
     1801 cctgctgcgc atcatgggcc tgatcgcgcc cccgcccggc gcgggtgcgc tgacgcgcgt
     1861 gcgcggcatc gccatcctgg tgggcgccgt gctggtgctg ctggccgtcg gcttcggcct
     1921 ggccaagctg gtgacgctcc cgttcggcgg cgtgggcacc ctgcccgcaa tcgcgatcgg
     1981 gctgctgatc gtggtcgcgg tgctgggcgt gctggccctg ttcggccgca ggcgccaggc
     2041 cagagccgcc gccgcgcgcg cgggctagcc ctcagtaggc ggacaccttc gtcctgccga
     2101 tgaccaccgg ctccatcggg gtgccggcgc cgccgctgga cgggtccccg agctgcccga
     2161 tccgctccac gactccgagc cccttcgtca cccgcccgag cgtggcgtac tccggcgcca
     2221 ggtccggcgc ctggcccgtg accacgaaga actggctgcc cgcggtgccc gccggctcct
     2281 caccggtctt ggccatggcc accatgccgc gctcgtagac ggtgtcggcc gggggcttgt
     2341 cggtggtcgt gtagcccgga ccgccctggc ccgacccggt cgggtcgccg ccctggatca
     2401 cgaacccggg cacgatccgg tggaagaccg tgccgtcgaa gaaccccttc cgggccagcg
     2461 acacgaacga ggcggccgtg gccggcgccg ccttctggtc gacctcgatc gtgaacgggc
     2521 cgcagttggt ccggaaggtc acgtcgtaac gcttgcccgg cgccaggttg gcggcgggcg
     2581 ggcgtcgctg ggtgccctcg gcccggggct tgggcgcctg gacccgccgg cacgcgtccg
     2641 agtcgcgggc agcggtcccc gcggcctggg tgtcgggggc cgccgtgtta tcggcggagg
     2701 cgccaccgtc gtcgtccccg ccacacccgg tggccagcag gacgccgacg agcccgaaga
     2761 ggaacgcacg tcgcacgaag cgcagagtag ccaggcgccg tcgccggccg taggttgttg
     2821 cggacgtgac cgccctctcc cctctgctcc agtcctacga ccaggtgatc ctggatctgg
     2881 acggctgcgt ccgggtgggc gacac
//
LOCUS       DASUPW010000328         5955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1463897, whole genome shotgun sequence.
ACCESSION   DASUPW010000328 DASUPW010000000
VERSION     DASUPW010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5955
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463897"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VFY44_07365"
     CDS             complement(<1..564)
                     /locus_tag="VFY44_07365"
                     /inference="COORDINATES: protein motif:HMM:NF024220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich protein"
                     /protein_id="HEX5910296.1"
                     /translation="MAAGASPATAETYPYVAPTTSDPYYTVVVPAGMTVAHIDLFGAQ
                     GGLLAGGKGARVQAALAVVPGEELRVFVGGQPADNRSAGGYNGGGESAGQQGTRGHGG
                     GGATDIRRAPYTEADRLAVAGGGGGSGSKEAAGGDSDGVGTAGGSDVTDGKGGRSGSE
                     GGAGGAAGTGSNDGGNAVPGGNAGGASG"
     gene            complement(657..1340)
                     /locus_tag="VFY44_07370"
     CDS             complement(657..1340)
                     /locus_tag="VFY44_07370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5910297.1"
                     /translation="MPSSDSPVLLITGASSGIGAATARAAVAEDWRVVLAARSQDKID
                     DLAAELGDGTALAVRCDVTEWDDQQAAVAAALEQFGRLDAFFANAGFGASRGFLEESP
                     EHWRAMVDTNVYGAALSIRASLAHFKKQDAGHFLVTGSAAGRVKLPGSLYSATKWAVH
                     AMADGLRQEVSDTKIKSTIIAPGMVDTPFFDEPKEGALQADDVARAVLYALSQPDHVD
                     VNEVLVRPN"
     gene            1367..1765
                     /locus_tag="VFY44_07375"
     CDS             1367..1765
                     /locus_tag="VFY44_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910298.1"
                     /translation="MASADMPTLTNRPSARLLGAVFTFTGVLHFARPAMYEAIMPPYL
                     PAHRELVYLSGAAEVAGAIAAFFPRLHPFARWWLIATLVAVFPANVHMAINPDDIKGL
                     PDVPQWALWLRLPIQVAFVAWVWHATRPAD"
     gene            complement(1762..2361)
                     /locus_tag="VFY44_07380"
     CDS             complement(1762..2361)
                     /locus_tag="VFY44_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910299.1"
                     /translation="MAQEPGDRYEPFPGLTKVPGWLWKRMGRGARIGAGVAGAVLVVG
                     VIVLIPVLADVRRDNRETERRERAAALAADIKRVNAIQRPRTATGPRDDPPAAVAALR
                     ARILADSNGRRGLNRARRVQCTLGAREGQVGRYSCTAVTARVINADGNKAYGVTGYPY
                     RAVVRFRTGGLTWCKVVGRAGEGSLYSRNPTPVPRACTG"
     gene            complement(2365..3963)
                     /locus_tag="VFY44_07385"
     CDS             complement(2365..3963)
                     /locus_tag="VFY44_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5910300.1"
                     /translation="MSESGYDYVIVGAGSAGCTLAARLTEDPGVRVLLLESGGKDRSP
                     NIKIPAAFGKQFHTKLDWDYSTVPQAGCANRELYSPRGRSLGGSSSMNAMLYVRGRPI
                     DYDLWESEGATGWGWQDVEPYFRRAEDHAGGADEIHGAGGPLRTMKVRSPHDLTNQFY
                     AAAEAAGIPYNRDYNGPEQDGVSPSQVTQHGGKRWSAADAYLKPNMKRPNLTVRTNAT
                     VLGLDLEGDRVTGVRLSGRRGRTETVRADRDVVLCAGAYGSPQLLMVSGIGPADHLAD
                     VGVDVRHDLPGVGQNLHDHPFCVCIWDAHDGPSLLDAEKPRALLEWVMRKSGPLTSPV
                     AEALGFVKTRPGLPAADVQFHMAPAYYVDHGETVYEGHACTIGPVLVTPKSRGQLTLR
                     TADPSDHPLIDPNALSEPEDVESMLAGMELAREIARSGPYAQSLGKELFPGPDVTDRQ
                     ALEDDLRQRVELIYHPVGTCRIGPLEAGGVVDPDLRVHGIEGLRVVDASVMPVVPGGN
                     TNAPTIMVAEKAADHILGKVPTPA"
     gene            3993..4883
                     /locus_tag="VFY44_07390"
     CDS             3993..4883
                     /locus_tag="VFY44_07390"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5910301.1"
                     /translation="MNEAEAEQADRRQELLALGAALVTVVLWASAFVGIRAAGEDLTG
                     GALSLGRLLVASVVLGVIVLARSEKLPARRDLGLVLVCGVLWFGIYNVALNEAERRVD
                     AGTAAMLVNVGPILIALLAGWLLKEGFPRTLLRGMAVAFAGVVVIGLATSQDGLTAST
                     GTFLCLVAAVTYAGGVVAQKPLLSRSSALAVTWAACTVGAVVCLPFGPKLVEEVGEAG
                     AGSIAWTVYLGVFPTAIAFTTWAYALTRSTAGRMGSTTYLVPPLAILMGWAILGETPP
                     ALALLGGVLCLTGAAMARRR"
     gene            complement(4880..5203)
                     /locus_tag="VFY44_07395"
     CDS             complement(4880..5203)
                     /locus_tag="VFY44_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX5910302.1"
                     /translation="MPDALRPLFDQVVIKELEPERMRKSGLLVPQGSSEMPPNEGIVL
                     AVGDGPPELPDFKMPVTPGDHVVFPRSAGVWVEIEDERLLVCQVREILGVIESTPARD
                     GAERG"
     gene            5251..>5955
                     /locus_tag="VFY44_07400"
     CDS             5251..>5955
                     /locus_tag="VFY44_07400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5910303.1"
                     /translation="MADYGQSLQFGVFITPDAADADGVVALSQVADTLGLDILSFQDH
                     PYQRRFLDTWTLLSNVAAQTSNVQLAPNVANLPLRPPAVLARSVATLDILSGGRAQLG
                     LGSGAFWDGIAALGGRKLTPGQGVDALTEALDVIRAMWSGERAVTVEGEYYSLTGAHP
                     GPAPRHDIEIWLGAYKPRMLALTGARADGWIPSMGYADPQELGPMNERIDQAAHEAGR
                     HPSQIRRMYNVNGQFGS"
BASE COUNT          737 a         2169 c         2189 g          860 t
ORIGIN      
        1 gcccgaggcg ccaccggcat tcccgccggg aacagcgttc ccgccgtcgt tgctcccggt
       61 gcccgcagca ccaccggcgc cgccctcgct gcccgagcgg ccgcccttgc cgtccgtcac
      121 atccgagccg ccggcggtac cgacgccgtc gctgtcgcca cccgccgcct ccttggagcc
      181 actgccgcca ccgccgcccg ccaccgccag gcggtcggcc tcggtgtacg gcgccctgcg
      241 gatgtcggtg gctccaccac cgccgtggcc ccgggtgccc tgctggccag ccgactcccc
      301 gccaccgttg tagccgccgg cgctgcggtt gtccgcaggc tgcccgccca cgaacacgcg
      361 cagctcctca ccgggaacga cggccagcgc ggcctgaacg cgcgctccct tgccaccggc
      421 caacaggccg ccctgcgctc cgaacaagtc gatgtgcgcg acggtcatcc cggccggcac
      481 caccaccgtg tagtaggggt cggaggtcgt gggggcgacg tacggatacg tctccgctgt
      541 cgcgggcgaa gcacccgccg ccacaccgac gatcgcgagg gcaacggctg ggactcccct
      601 gagcatccgc atcgcatcac cgtaagtggg atcccgctat cccgccaacg ggggactcag
      661 ttcgggcgca caagcacttc gttcacgtcc acgtggtcgg gctgtgacag cgcgtagagc
      721 accgcccggg ccacgtcgtc ggcctgcagc gcgccctcct tgggctcgtc gaagaagggc
      781 gtgtccacca tgccgggggc gatgatcgtg ctcttgatct tcgtgtccga gacctcctgg
      841 cgcaggccgt cggccatggc gtgcacggcc cacttggtgg ccgagtacag cgagccgggg
      901 agcttgacgc gcccggccgc ggagccggtg accaggaagt ggcccgcgtc ctgcttcttg
      961 aagtgggcga gcgaggcgcg gatggacagc gccgcgccgt agacgttggt gtcgaccatt
     1021 gcgcgccagt gctcggggct ctcctcgagg aagccgcgcg aggcgccgaa gccggcgttg
     1081 gcgaagaacg cgtccaggcg gccgaactgc tcgagcgcgg cggccaccgc ggcctgctgg
     1141 tcgtcccact cggttacgtc gcagcgcacg gccagcgcgg tgccgtcgcc cagctcggcg
     1201 gccaggtcgt cgatcttgtc ctgcgagcgg gcggccagca ccacgcgcca gtcctcggcg
     1261 accgcggcgc gggcggtggc cgcgccgatg ccgctgctgg cgccggtgat caggaggacg
     1321 gggctgtcag aggaaggcat gcctctggtc tagcatgccg cggctcatgg cctccgccga
     1381 catgcccacg ttgaccaacc gcccgtccgc ccgcctgctg ggggccgtgt tcacgttcac
     1441 cggcgtgctg cacttcgcgc ggcccgccat gtacgaggcg atcatgccgc cgtacctgcc
     1501 cgcgcaccgg gagctggtct atctgagcgg cgcggccgag gtggccggcg cgatcgcggc
     1561 cttcttcccg cgcctgcacc cgttcgcccg atggtggctg atcgcgacgc tggtggccgt
     1621 cttcccggcc aacgtgcaca tggcgatcaa ccccgacgac atcaaggggt tgccggacgt
     1681 cccgcagtgg gcgctgtggc tgcgcctgcc gatccaggtg gcgttcgtcg cctgggtgtg
     1741 gcacgccacg cggcccgccg actagcccgt acaggcccgg gggacggggg tggggttgcg
     1801 cgagtagagg ctgccctcgc cggcgcgccc cacgaccttg caccacgtca gcccgccggt
     1861 gcggaaccgc accacggcgc ggtaggggta gccggtcacg ccgtacgcct tgttcccgtc
     1921 cgcgttgatc acccgggcgg tgaccgccgt gcacgagtag cgcccgacct gcccttcccg
     1981 ggcgccgagg gtgcactgca cccgccgggc cctgttcagg ccccggcggc cgttggagtc
     2041 ggccaggatc cgggctcgga gcgcggcgac cgcggcgggc gggtcatccc gcggccccgt
     2101 ggcggtgcgc gggcgctgga tggcgttcac gcgcttgatg tcggcggcca gggcggcggc
     2161 gcgctcgcgc cgctcggtct cgcggttgtc ccggcgcacg tcggcgagca cggggatcag
     2221 gacgatcact cccacgacca ggacggcgcc ggccacgccc gccccgatgc gcgcgcctcg
     2281 gcccatccgc ttccacagcc agccggggac cttcgtcagc ccgggaaacg gctcgtagcg
     2341 gtccccgggc tcctgcgcca tcgctcaggc cggggtgggg accttgccca ggatgtggtc
     2401 ggcggccttc tcggccacca tgatggtggg cgcgttggtg ttgccgccgg gcaccaccgg
     2461 catgaccgag gcgtccacca cgcgcagccc ctcgatcccg tggacgcgca ggtccgggtc
     2521 gaccacgccg cccgcctcca gcggcccgat gcggcaggtg cccacggggt ggtagatcag
     2581 ctcgacgcgc tggcgcaggt cgtcttcgag cgcctggcgg tcggtcacgt ccgggccggg
     2641 gaagagctcc ttgcccaggc tctgcgcgta cgggcccgag cgcgcgatct cgcgcgccag
     2701 ctccatgccc gccagcatcg actcgacgtc ctcgggctcg ctgagcgcgt tggggtcgat
     2761 cagcgggtgg tccgacgggt ccgccgtgcg cagcgtcagc tgtccgcgcg acttcggggt
     2821 gaccagcacg gggccgatcg tgcaggcgtg gccctcgtag acggtctcgc cgtggtccac
     2881 gtagtaggcc ggggccatgt ggaactggac gtcggcggcc ggcagcccgg ggcgcgtctt
     2941 gacgaagccg agcgcctcgg cgaccgggga ggtcagcggg cccgacttgc gcatgaccca
     3001 ttccagcagc gcccgcggct tctcggcgtc cagcaggctg ggcccgtcgt gggcgtccca
     3061 gatgcagacg cagaaggggt ggtcgtgcag gttctggccc acgcccggga ggtcgtggcg
     3121 cacgtccacg ccgacgtcgg ccaggtggtc cgcggggccg atccccgaca ccatcagcag
     3181 ctgcggcgac ccgtaggcgc cggcgcacag gaccacgtcg cgatcggccc ggacggtctc
     3241 ggtcctcccg cggcggcccg agagccgcac gcccgtcacg cgatcgccct ccaggtccag
     3301 ccccaggacg gtcgcgttgg tgcgcacggt caggttgggc cgcttcatgt tcggcttcag
     3361 gtaggcatcg gcggcgctcc agcgcttgcc gccgtgctgc gtgacctgtg acgggctcac
     3421 accgtcctgc tccgggccgt tgtagtcgcg gttgtaggga atgcccgccg cctcggccgc
     3481 ggcgtagaac tggttggtca gatcatgcgg agagcgaacc ttcatcgtgc gcagcgggcc
     3541 cccggcgccg tggatctcgt ccgcgccgcc cgcgtggtcc tcggcgcggc ggaagtaggg
     3601 ctccacgtcc tgccagcccc agccggtcgc gccctcgctc tcccacaggt cgtagtcgat
     3661 cgggcggccg cggacgtaga gcatcgcgtt catggacgac gacccgccga gcgaacggcc
     3721 ccgcggcgag tagagctcac gattggcgca tccggcctgg gggacggtgg agtagtccca
     3781 gtcgagcttg gtgtggaact gcttgccgaa cgcggcgggg atcttgatgt tggggctgcg
     3841 gtccttgccg ccggactcca ggagcaggac cctgacgccg gggtcctcgg tcaggcgcgc
     3901 cgccagggtg cagccggccg atccggcgcc gacgatgacg tagtcgtatc cgctctcgct
     3961 cactcgcggc cgaggctact agcgtggcgg ccttgaacga agccgaagcc gaacaggccg
     4021 accgccgcca ggagctgctg gcgctcggtg ccgccctggt gacggtcgtc ctctgggcca
     4081 gcgcgttcgt gggcatccgg gcggcggggg aggacctgac cggcggcgcg ctctccctgg
     4141 gccggctgct ggtggcgagc gtggtgctgg gcgtgatcgt gctggcccgc agcgagaagc
     4201 tccccgcgcg gcgcgacctg ggcctggtgc tggtgtgcgg cgtgctctgg ttcgggatct
     4261 acaacgtggc gctgaacgag gccgagcggc gtgtggacgc ggggaccgcg gcgatgctgg
     4321 tcaacgtcgg gccgatcttg atcgccctgc tggccggatg gctgctcaag gagggctttc
     4381 cgcgcaccct gctgcggggc atggccgtgg cgttcgccgg cgtcgtggtg atcggcctgg
     4441 ccacctccca ggacggcctg accgccagca ccgggacctt cctctgcctg gtggccgccg
     4501 tcacctacgc gggcggcgtg gtggcccaga agccgctgct gtcgcgctcg tcggcgctgg
     4561 cggtgacctg ggccgcctgc accgtcggcg cggtggtctg cctgccgttc gggcccaagc
     4621 tggtcgagga ggtgggggag gccggtgcgg gatcgatcgc ctggaccgtc tacctgggcg
     4681 tgttcccgac cgcgatcgcc ttcaccacct gggcctacgc gctgacgcgc agcaccgcgg
     4741 gccggatggg gtcgaccacc tacctggtgc cgccgctggc gatcctgatg gggtgggcga
     4801 tcctggggga gacgccgccc gcgctggcgc tgctgggcgg ggtgctctgc ctgacgggtg
     4861 ccgcgatggc gcggcgccgc tagccgcgct cggcgccgtc gcgggccggc gtggactcga
     4921 tcacgcccag gatctcccgt acctggcaca ccagcaggcg ctcgtcctcg atctccaccc
     4981 acacgccggc ggagcggggg aagaccacgt ggtcgcccgg ggtcacgggc atcttgaagt
     5041 ccgggagctc gggcggcccg tcgccgacgg cgagcacgat gccctcgttg ggcggcatct
     5101 cgctgctgcc ctggggcacc agcagcccgg acttgcgcat gcgctcgggc tcgagctcct
     5161 tgatgaccac ctggtcgaag agggggcgga gggcgtcggg cacggagacg aaggtagtgc
     5221 aacccggccc cccgactagg atcggcgtcc gtggcggact acggccagag cctgcagttc
     5281 ggggtgttca tcaccccgga cgccgccgac gccgacggcg tggtggcgct ctcgcaggtg
     5341 gccgacacgc tgggcctgga catcctgagc ttccaggacc acccgtacca gcggcgcttc
     5401 ctggacacgt ggacgctgct gagcaacgtg gccgcccaga ccagcaacgt gcagctggcg
     5461 ccgaacgtgg ccaacctgcc gctgcgcccg cccgcggtgc tggcccgcag cgtcgccacg
     5521 ctggacatcc tgagcggcgg ccgcgcccag ctgggcctgg gctccggcgc gttctgggac
     5581 ggcatcgcgg cgctgggcgg gcgcaagctc acgcccggcc agggcgtgga cgcgctgacc
     5641 gaggcgctgg acgtgatccg ggcgatgtgg tcgggcgagc gcgcggtgac cgtcgagggc
     5701 gagtactact cgctgaccgg cgcgcacccc ggcccggcgc cccggcacga catcgagatc
     5761 tggctgggcg cctacaagcc gcgcatgctg gccctgaccg gcgcccgcgc cgacggctgg
     5821 atccccagca tgggctacgc cgacccgcag gagctggggc ccatgaacga gcgcatcgac
     5881 caggcggccc acgaggccgg gcgccacccg agccagatcc gccgcatgta caacgtcaac
     5941 ggccagttcg gctcc
//
LOCUS       DASUPW010000329         2540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_909742, whole genome shotgun sequence.
ACCESSION   DASUPW010000329 DASUPW010000000
VERSION     DASUPW010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909742"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1970
                     /locus_tag="VFY44_07405"
     CDS             <1..1970
                     /locus_tag="VFY44_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Zn-binding domain-containing protein"
                     /protein_id="HEX5910304.1"
                     /translation="SARALYLYPTKALAQDQARALHALKLPHLRPAIYDGDTPKEERR
                     AIRSRSNLILTNPDMLHVGALPNHSQWGDLLANLAWVVVDEAHTYRGVFGSHVANVLR
                     RLRRLALAYGTEPRFMLASATIANPVELAERLVGDEVRLVARDGAPRAERRIAMWNPP
                     LVDERTGARASALGEAADLLADLVETETRTICFLRSRRGVELIQQFTKLRLEDRGRAD
                     LAERVAPYRAGYTPMQRREIEQRLVSGELLAVVTTSALELGIDVGDLDAAICVTFPGT
                     VASLRQMWGRAGRRNTGLALYVAGADALDQFFCRHPDQFLDRPVESAILDHESEEIFL
                     AHLCAAAYELPLSPGDEEFLGDRWEPAARRLVSMGQLRERAGRYLPRGEGYPAGRIAL
                     RSSSPDSVAVIESEGGEVIGSVEAARASGAVHDGSVYLHMGRSYLVEHLDLDNRRALV
                     TPFTGDFYTQSKHETDTFIEEVVDRREVADCLLSFGTVNVTEQVTGYQRKRVSDHEVL
                     DLVALDMPEQHFTTQALWWEMPEQALAEDFPLDVLQGSLHAAEHAQIAVLPLIAMCDR
                     WDIGGLSTAFHQQTGRPTVFIYDGHPGGVGITRLGFQQFERLVANATALISECPCRAG
                     CPSCVQSPKCGNLNEPLNKNGAVELLRRLAS"
     gene            2019..>2540
                     /locus_tag="VFY44_07410"
     CDS             2019..>2540
                     /locus_tag="VFY44_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910305.1"
                     /translation="MRLLLATCLAGLALCPATAAAVGSSTPSGGAATPTEGGAHYGET
                     PKVHGLASLPHLRAFSVSRPTLTATVSFRIASKLPVRDVRLQLLNARGSAVKTLKLGT
                     RKAGKVHKVIVSRTGLRAGSYRVRITARQLRTAGIASVRRVTVPKPPRPKPVPPAPPA
                     APLGTHVFPVRGTY"
BASE COUNT          334 a          970 c          893 g          343 t
ORIGIN      
        1 gctcggcgcg cgcgctgtac ctgtacccga ccaaggccct ggcccaggac caggcgcggg
       61 cgctgcacgc gctgaagctg ccccacctgc gaccggcgat ctacgacggc gacacgccga
      121 aggaggagcg ccgcgccatc cgctcgcgct ccaacctgat cctgaccaac cccgacatgc
      181 tgcacgtggg ggcgctgccc aaccactccc agtggggcga cctgctggcc aacctggcct
      241 gggtggtggt ggacgaggcc cacacctacc gcggcgtgtt cggctcccac gtggccaacg
      301 tgctgcgccg cctgcggcgg ctggccctgg cctacggcac cgagccgcgg ttcatgctgg
      361 ccagcgccac catcgccaac ccggtggagc tggccgagcg gctggtgggc gacgaggtgc
      421 ggctggtggc ccgcgacggc gccccgcgcg ccgagcgccg gatcgccatg tggaacccgc
      481 ccctggtgga cgagcgcacc ggcgcccgcg cctccgccct gggcgaggcg gcggacctgc
      541 tggccgacct ggtcgagacc gagacccgca ccatctgctt cctgcgctcg cggcggggcg
      601 tcgagctgat ccagcagttc accaagctcc gcctggagga ccgcgggcga gccgacctgg
      661 ccgagcgggt ggccccctac cgcgccggct acaccccgat gcagcggcgc gagatcgagc
      721 agcggctggt gtccggcgag ctgctggccg tggtcaccac cagcgcgctg gagctgggca
      781 tcgacgtcgg cgacctggac gccgccatct gcgtgacgtt ccccggcacg gtggcctcgc
      841 tccgccagat gtggggccgc gccgggcggc gcaacacggg gctggccctg tacgtggcgg
      901 gcgccgacgc gctggaccag ttcttctgcc gccatcccga ccagttcctg gaccgccccg
      961 tggagtcggc catcctggac cacgagtccg aggagatctt cctggcccac ctgtgcgccg
     1021 ccgcctacga gctgcccctg tcgccgggcg acgaggagtt cctgggcgac cggtgggagc
     1081 cggccgcgcg gcggctggtg agcatgggcc agctgcgcga gcgcgccggg cgctacctgc
     1141 cccgcggcga ggggtacccg gccggccgca tcgccctgcg ctcgtcgtcg cccgactcgg
     1201 tggcggtcat cgagtccgag ggcggcgagg tgatcggctc ggtggaggcc gcgcgcgcct
     1261 ccggcgccgt gcacgacggc tcggtgtacc tgcacatggg ccgctcgtac ctggtcgagc
     1321 acctggacct ggacaaccgc cgggcgctgg tcacgccgtt caccggcgac ttctacaccc
     1381 agtccaagca cgagaccgac accttcatcg aggaggtggt ggaccgccgc gaggtggccg
     1441 actgcctgct gagcttcggc accgtgaacg tgaccgagca ggtcaccggc taccagcgca
     1501 agcgcgtgtc cgaccacgag gtcctggacc tggtggcgct ggacatgccc gagcagcact
     1561 tcaccaccca ggcgctgtgg tgggagatgc ccgagcaggc cctggccgag gacttcccgc
     1621 tggacgtgct ccagggctcg ctgcacgccg ccgagcacgc ccagatcgcc gtgctgccgc
     1681 tgatcgcgat gtgcgaccgg tgggacatcg gcggcctgtc caccgccttc caccagcaga
     1741 ccggccgccc gaccgtgttc atctacgacg gccacccggg cggcgtgggc atcacgcgcc
     1801 tgggcttcca gcagttcgag cgcctggtcg cgaacgccac cgcgctgatc tcggagtgcc
     1861 cctgccgggc cggctgcccc tcgtgcgtgc agtcgcccaa gtgcggcaac ctgaacgagc
     1921 cgctgaacaa gaacggggcc gtcgagctgc tgcgccggct cgcatcctga gttgcaggcc
     1981 cttcaagttc cgcggtctcc ggtcgagggg gatcttcgat gcgcctcctg ctcgccacct
     2041 gcctcgccgg cctggccctg tgcccggcca ccgcggcggc cgtgggttcg agcaccccgt
     2101 cgggcggggc cgcgacgccg accgaggggg gcgcgcacta cggcgagacc cccaaggtgc
     2161 acgggctggc gtcgttgccg catctcaggg ccttctccgt gtcccggccc acgctgaccg
     2221 ccacggtcag cttccggatc gcatcgaagc tcccggtcag ggacgtccgg ctccagctgc
     2281 tgaacgccag gggctcggcc gtgaagacgc tgaagctggg cacccgcaag gccggcaagg
     2341 tgcacaaggt catcgtgtcc cgcacgggcc tgcgcgcggg ctcctaccgg gtccggatca
     2401 cggccaggca gctccgcacg gccggcatcg cctcggtcag gcgcgtgacc gtgcccaagc
     2461 cgcccaggcc caagcccgtg ccgcccgctc cgccggcggc cccgctgggc acgcacgtct
     2521 tccccgtccg cggcacctac
//
LOCUS       DASUPW010000330         5138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_79114, whole genome shotgun sequence.
ACCESSION   DASUPW010000330 DASUPW010000000
VERSION     DASUPW010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79114"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFY44_07415"
     CDS             complement(<1..230)
                     /locus_tag="VFY44_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910306.1"
                     /translation="MRGRALALPLAALVLAVALLGWYVAAGGADYAEVDGAADPCAAR
                     ASVDRPVAAELEALAERVVLVGLDETACKLGVS"
     gene            complement(227..1582)
                     /locus_tag="VFY44_07420"
     CDS             complement(227..1582)
                     /locus_tag="VFY44_07420"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5910307.1"
                     /translation="MKPAVIALSVAVGVVLADSAVVTLALPDILRQYETTVGQVSWVL
                     TAFNLVLALAAVPAARLCVHRDPGALGAVGLVVFALCCVACAAAPSLEVLLAARVGQA
                     LGGAVVIAASLEMLVAASGSESQGAARWAGAGVAGAALGPVAGGLLTEAISWQSIFIV
                     QVPLVLAAVPTALAWRGHAGRDHDVSMPSDRPHVAANAALALISAALTAALFLLVLLL
                     VEGWRRSPAEAALIVTVIPLAALAAGPLARAARAGTRSEAVAGAVAVAGGLAALALLP
                     GASAGWTLAPQALVGLGLGLTVDSLTAAALHDRVPRALHGGWTIAARHGGIVVGLLIL
                     TPVFTADLRDAREPAQEAIAAQVLDSRLPASSKLALAEGLGEQLAGEGGRVPDLSPAF
                     ARADLPAGETVRAAALERGLDDQLERAATGAFSDAFLLAGLLALLAVVPALFLRGRGE
                     S"
     gene            complement(1611..3143)
                     /locus_tag="VFY44_07425"
     CDS             complement(1611..3143)
                     /locus_tag="VFY44_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910308.1"
                     /translation="MRKLALGSLVVAVLFAFAPPGARADVRFELKGQWTCKDGSTVKP
                     MAGARVELFRERSYWRDEKITTRHLGADGRYSIPVKASDNFNLYVKLLLHDDDGVELE
                     NWYSPWTWETSTSTKKSRSGTVDLGTWQIGKDGSGTPKCAIWQGVHNAYANFRQVIGA
                     RPPSNIKIEGEFPCCGTPFTTTNSIRWPGGYAVRANYSVPFHEFAHSFRHSYDGNFGH
                     FLVDAARYQYPQFHSSCKVTNQGFAFNEGWAEYWAGDYSTCSPATNYNQEGNVAAELA
                     RLERCSSRPAMVRVLRESRGQIHSLAEFQTRFNTLLGGCAQPLTTTTGGGGDLVLTAQ
                     QQITELQRQIAAQRKLVASLNRQAAAAKRRARNPGRCSAGGCNAAMEKLIEPSALAAQ
                     AQQARLVLDRLEEGLAAARKAGFDPARQVTLGDSLVKGEDAFEKANQKILVAGLQKGL
                     REIKTEPGFNRGEKSELFKTMDRRADGLARARRRGQETPASVETLFSAPSSPTEEATK
                     VR"
     gene            complement(3198..3446)
                     /locus_tag="VFY44_07430"
     CDS             complement(3198..3446)
                     /locus_tag="VFY44_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910309.1"
                     /translation="MVTIVVCLLAFLALGYALGRSRWTHLVPAALALYILLGTFFEGV
                     EDASAWLYFLGVASASLVAGLVGVMLRERSHRIPSATS"
     gene            complement(3478..>5138)
                     /locus_tag="VFY44_07435"
     CDS             complement(3478..>5138)
                     /locus_tag="VFY44_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910310.1"
                     /translation="TCNATEVANHPTPGGFRVWRYVDRSGRTCAYYDTSLSAPANVAR
                     LAAGPSPGVAVLDMSNPERPVETDRLTTLAMLSPHESLNLNERRGLLAAVTGNAATLP
                     GAVAIYDVSQDCRHPRLRSQLASSNGHESGFSPDGNTFWAGGAAGYIRAIDVADPGAP
                     REIWTGAYYSHGLNVSDDGNTLYQTDPVNGNVGILDVSDVQARKPNPRVRELDRLTWD
                     TVSIPQNSVPLTIKGKRFLFEFDEFAFRFNPATVDNKVGAARLIDLSRPDRPRIASNI
                     RLEVNQQANHAAVAGDPTALPDANTALGYSAHYCAAPRRADPGIVACSFLNSGLRVFD
                     VRDPEDPREVAYFLAPPNAGKVPGFLPGNLAFSQPAFDPQRRTVWYTDAGSGFYAVKL
                     SAAAWPADAKAPPCLARRSSIGPDHIGRLRLGMTRARMRRLPVRAPRRSARTYRYCVK
                     QAAGGVTAAFSSKARRGKARLITTTAKGSGNRRATVGTSAARFRRAFPNRVRVGRGLY
                     RLGRGSTRIAGIRKGRVRFYGVTSKAVTRRPALMRRYLRVAGLR"
BASE COUNT          699 a         1883 c         1910 g          646 t
ORIGIN      
        1 gacacgccca gcttgcacgc cgtctcgtcc agccccacca gcaccacccg ctcggccagc
       61 gcctccagct ctgccgccac gggccggtcg accgaggcac gcgccgcgca cggatccgcc
      121 gccccgtcca cctcggcgta gtcggcgccc ccggccgcca cgtaccaccc gagcaacgca
      181 acggccagca ccagcgcggc cagcggcagc gccagcgccc gccccctcac gattcacccc
      241 gcccgcgcag gaacagcgcc ggcaccacgg ccagcagcgc cagcagcccg gccaggagga
      301 acgcgtcgct gaacgcaccc gtggcggcgc gctcgagctg gtcgtccagc ccccgctcca
      361 gcgcggccgc acgaacggtc tcccccgccg gcaggtcggc gcgcgcgaac gcgggcgaca
      421 ggtcagggac ccgcccgccc tcgccggcca gctgctcccc cagcccctcg gccagcgcca
      481 gcttcgacga ggccggcagc cgtgaatcga gcacctgcgc ggcgatggcc tcctgcgccg
      541 gctcgcgcgc gtcgcgcagg tcggcggtga acaccggcgt gaggatgagc agccccacca
      601 cgatgccgcc gtggcgggcg gcgatggtcc agccgccgtg cagcgcccgc ggcacccggt
      661 cgtgcagagc ggcggccgta agcgaatcga cggtgaggcc caggcccagc cccacgagcg
      721 cctgcggcgc cagcgtccac cccgccgacg cccccggcag cagcgccagc gccgccagcc
      781 cgcccgccac cgcgacggcc cccgccaccg cctccgagcg cgtgcccgcg cgcgccgccc
      841 gcgccagcgg cccggccgcg agggcggcca gcgggatcac ggtgacgatg agcgcggcct
      901 cggccggaga gcgccgccag ccctcgacca gcagcagcac cagcaggaac agcgcggcgg
      961 tcagcgccgc cgagatgagc gccagcgccg cgttggcagc cacgtgcggg cggtcggagg
     1021 gcatcgagac gtcgtggtcg cgcccggcgt ggccgcgcca ggccagcgcc gtgggcaccg
     1081 cggccagcac cagcggcacc tgcacgatga agatcgactg ccacgagatg gcctcggtga
     1141 gcaggccgcc cgccaccggc cccagcgcgg cgccggcgac cccggccccc gcccaccggg
     1201 ccgctccctg cgactccgag cccgacgcgg ccaccagcat ctccagcgac gcggcgatga
     1261 ccaccgcccc gcccagcgcc tgccccacgc gcgcggccag cagcacctcg agcgacgggg
     1321 ccgccgcgca cgccacgcag cagagggcga acaccaccag gcccacggcg cccagggcgc
     1381 cggggtcgcg gtgcacgcac agccgcgcgg cgggcaccgc ggccagcgcc aggaccaggt
     1441 tgaaggccgt gagcacccac gacacctgcc ccaccgtggt ctcgtactgg cgcaggatgt
     1501 ccggcagcgc cagcgtgacc acggccgagt cggccagcac cacccccacg gccacggaca
     1561 gcgcgatgac ggcgggcttc atcgcccgcc attagagacg atcgcgcgcc ctagcggacc
     1621 ttcgtcgcct cctccgtcgg cgaggacggc gccgagaaca gcgtctccac gctggcgggt
     1681 gtctcctggc cgcgccggcg cgcgcgggcc aggccgtcgg cgcgcctgtc catggtcttg
     1741 aacagctccg acttctcgcc gcggttgaac cccggctcgg tcttgatctc gcgcagcccc
     1801 ttctggaggc cggcgaccag gatcttctgg ttggccttct cgaacgcgtc ctcgcccttg
     1861 accagcgagt cgcccagggt gacctgcctg gcggggtcga agccggcctt gcgcgcggcg
     1921 gccaggccct cctcgagccg atcgagcacc agcctggcct gctgggcctg cgcggccagg
     1981 gccgagggct cgatcagctt ctccatggcc gcgttgcagc ccccggcgct gcagcggccg
     2041 gggttgcgcg cgcgccgctt ggccgcggcg gcctggcggt tcagcgaggc caccagcttg
     2101 cgctgggcgg cgatctggcg ctggagctcg gtgatctgct gctgggcggt gagcaccagg
     2161 tcgccgccac ccccggtggt ggtggtcagc ggctgcgcgc agccgcccag aagggtgttg
     2221 aagcgggtct ggaactcggc cagcgagtgg atctgcccgc ggctctcgcg cagcacccgg
     2281 accatggccg ggcggctgga gcaacgctcc aggcgggcca gctcggcggc cacgttgccc
     2341 tcctggttgt agttggtcgc cggcgagcag gtggagtagt caccggccca gtactcggcc
     2401 caaccctcgt tgaaggcgaa gccctgattg gtgaccttgc acgacgagtg gaactgcggg
     2461 tactggtagc gcgccgcgtc gaccaggaag tgcccgaagt tgccgtcgta gctgtggcgg
     2521 aacgagtgcg cgaactcgtg gaagggcacc gagtagttgg cccggaccgc gtagccgccg
     2581 ggccagcgga tgctgttggt ggtggtgaac ggggtgccgc agcacgggaa ctcaccctcg
     2641 atcttgatgt tggacggcgg ccgcgcaccg atgacctggc ggaagttggc gtaggcgttg
     2701 tgcacgccct gccagatcgc gcacttgggc gtgcccgagc cgtccttgcc gatctgccac
     2761 gtgcccaggt ccaccgttcc cgagcggctc ttcttcgtcg acgtgctggt ctcccaggtc
     2821 cacggcgagt accagttctc cagctcgacc ccgtcgtcgt cgtgcagcag cagcttcacg
     2881 tacaggttga agttgtcgct ggccttgacg gggatcgagt agcgcccgtc ggcgcccagg
     2941 tggcgggtcg tgatcttctc gtcgcgccag tacgagcgct cgcggaacag ctcgacgcgg
     3001 gcgccggcca tcggcttgac cgtgctgccg tccttgcacg tccactggcc cttgagctcg
     3061 aaccgcacgt ccgcgcgggc gccgggtggg gcgaaggcga acagcacagc caccacgagc
     3121 gaacccagcg cgagctttct catgtcgcga ccgtacgccc gccgggcggc gggcgcaagg
     3181 tcccgtacta gtacgcccta ggaggtggcc gagggaatcc ggtgcgagcg ctcgcgcagc
     3241 atcacgccga ccagcccggc gaccaggctg gccgaggcca cgcccaggaa gtacagccac
     3301 gcgctggcgt cctcgacgcc ctcgaaaaag gtgcccagga ggatgtagag cgccagcgcg
     3361 gcggggacca ggtgggtcca gcgggaacgg ccgagggcgt agcccagtgc caggaacgcg
     3421 agcaggcaga cgacgatggt gaccatgcct cgggtatcgg cgttcgcccc ggcggcttta
     3481 gcgcaggccc gcgacgcgca ggtaccggcg catcagcgcc ggccggcggg tgacggcctt
     3541 ggaggtgacg ccgtagaagc ggacgcggcc cttgcggatg ccggcgatgc gggtgctgcc
     3601 gcggccgagg cggtagaggc cgcggccgac gcggacgcgg ttggggaagg ccctgcgaaa
     3661 gcgcgcggcc gaggtgccga ccgtcgcgcg ccggttgccc gaccccttgg ccgtggtggt
     3721 gatgagccgg gccttgcccc gtcgggcctt gctcgagaac gccgcggtga cgccgccggc
     3781 cgcctgcttg acgcagtagc ggtaggtgcg ggcgctgcgt cgcggcgcgc ggacgggcag
     3841 gcgccgcatg cgcgcgcggg tcatcccgag cctgagccgc ccgatgtggt cggggccgat
     3901 cgacgagcgg cgggccaggc acgggggtgc cttggcgtcc gcgggccacg cggcggcgga
     3961 gagcttgacc gcgtagaagc ccgagcccgc gtccgtgtac cagacggtgc gccgctgggg
     4021 atcgaaggcc ggctgggaga acgccaggtt gccgggcagg aagccgggga ccttgcccgc
     4081 gttggggggc gcgaggaagt acgcgacctc gcgcgggtcc tcgggatcgc ggacgtcgaa
     4141 cacccgcagg ccggagttca ggaacgagca ggccacgatg ccggggtcgg cgcgccgcgg
     4201 cgcggcgcag tagtgcgcgc tgtagccgag cgcggtgttg gcgtccggca gcgcggtcgg
     4261 gtcgcccgcg acggccgcgt ggttggcctg ctggttcacc tcgagccgga tgttggaggc
     4321 gatccgcggc cggtcgggcc ggctgaggtc gatcagccgg gcggcgccga ccttgttgtc
     4381 cacggtcgcg gggttgaagc ggaacgcgaa ctcgtcgaac tcgaacagga agcgcttgcc
     4441 cttgatggtc agcggcacgc tgttctgggg gatcgagacc gtgtcccacg tcaggcggtc
     4501 gagctcgcgc accctcgggt tcggcttgcg ggcctggacg tcgctgacgt cgaggatgcc
     4561 cacgttcccg ttgaccggat cggtctggta gagcgtgttg ccgtcgtcgc tgacgttcag
     4621 cccgtgcgag tagtaggcgc cggtccagat ctcgcgcggc gcgcccgggt cggccacgtc
     4681 gatcgcgcgg atgtagccgg cggcgccgcc cgcccagaag gtgttgccgt cgggcgagaa
     4741 gccgctctcg tggccgttgc tgctggccag ctgggagcgc agcctggggt ggcggcagtc
     4801 ctggctgacg tcgtagatcg ccaccgcccc cggcagcgtc gcggcgttgc ccgtcacggc
     4861 ggccagcagc ccgcgccgct cgttgaggtt cagggactcg tggggcgaga gcatggccag
     4921 cgtggtcagc cggtcggtct cgaccgggcg ctcggggttg ctcatgtcca ggacggcgac
     4981 gcccggtgac ggccccgcgg ccagccgggc cacgttggcg ggagcggaca gcgaggtgtc
     5041 gtagtaggcg caggtccggc cgctgcggtc cacgtagcgc cacacgcgga acccgccggg
     5101 ggtcgggtgg ttggccacct cggtggcgtt gcacgtcc
//
LOCUS       DASUPW010000331         2056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1741490, whole genome shotgun sequence.
ACCESSION   DASUPW010000331 DASUPW010000000
VERSION     DASUPW010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1741490"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VFY44_07440"
     CDS             <1..271
                     /locus_tag="VFY44_07440"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HEX5910311.1"
                     /translation="TVIPDILANAGGVTVSYFEWAQNRSGVRWSAEDVAERLEQKMVE
                     QSEEVWDCARDRDVTLRTAAYVVGLERISESVNATGSVEQFRNGR"
     gene            343..1680
                     /locus_tag="VFY44_07445"
     CDS             343..1680
                     /locus_tag="VFY44_07445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEX5910312.1"
                     /translation="MYFFGALGGMLFGYDTGVISGAILFIPNDFELTPFLEGAVVAGL
                     LLGAMAGAATAGRLSDRLGRRKVILAAAVIFTVGALGAALAPTVGVLVAARVVLGVAV
                     GAAALIVPLYLSEVAPTSIRGAVTSLNQLMVVVGILIAFIVNAALASSENWRLMLGLA
                     VIPSVALFVGMWSMPETPRWLIRRGREDDAREVLAQTREESEIDDEIREIREVEEAES
                     GGAGLRAAFAPWVRPALLVAVGLAVFQQLVGINTIIYFAPTTLTNVGYGPSGAIYANL
                     VIGVLNVAATVVAVRIVDRVGRKPLLLAGLAGMVTSLTVLGLSNLLMAEPNSAGDPLA
                     IVTLACLGGFIVSFAATWGPVVWVMLPEVLPLSVRGSAMGVAIFLHWGANFLVAQTFP
                     ILLDEFEAGPVFLGYAVIGVIAAVFVTTKVQETKGRSLEAIEEELQDEAPAAA"
BASE COUNT          262 a          703 c          770 g          321 t
ORIGIN      
        1 cacggtgatc cccgacatcc tggccaacgc cggcggggtg accgtgtcct acttcgagtg
       61 ggcccagaac cgcagcggcg tgcgctggag cgccgaggac gtggccgagc ggctggagca
      121 gaagatggtc gagcagtccg aggaggtctg ggactgtgcc cgcgaccgcg acgtcacgct
      181 gcgcaccgcg gcctacgtgg tcgggctgga gcgcatctcc gagtcggtga acgcgaccgg
      241 gtcggtcgag cagttccgca acggccgctg acgcgcttga gatgaccgcg ctccgggcaa
      301 ggcccggcca tggagacgag gagcctgcga gagaacgccc gggtgtactt cttcggagcg
      361 ctcgggggga tgctgttcgg ctacgacacc ggcgtcatct ccggcgcgat cctgttcatc
      421 ccgaacgact tcgagctgac gccgttcctg gagggcgccg tggtggccgg gctgctgctg
      481 ggcgcgatgg ccggcgcggc cacggcggga cggctgtccg accgcctggg ccggcgcaag
      541 gtgatcctgg cggccgccgt gatcttcacc gtgggcgcgc tgggcgcggc gctggccccc
      601 acggtcggcg tcctggtggc cgcgcgcgtg gtgctgggcg tggcggtggg cgcggccgcg
      661 ctgatcgtgc cgctgtacct gtccgaggtg gctcccacct cgatccgcgg cgcggtgacg
      721 tcgctgaacc agctgatggt ggtcgtgggc atcctgatcg cgttcatcgt gaacgcggcg
      781 ctggcctcgt cggagaactg gcggctgatg ctgggcctgg ccgtgatccc gtcggtcgcc
      841 ctgttcgtgg gcatgtggag catgcccgag accccgcgct ggctgatccg gcgcgggcgc
      901 gaggacgacg cccgcgaggt gctggcgcag acgcgcgagg agtccgagat cgacgacgag
      961 atcagggaga tccgcgaggt cgaggaggcc gagtccggcg gcgccggcct gcgcgcggcg
     1021 ttcgcgccct gggtgcgccc ggcgctgctg gtggccgtcg gcctggccgt gttccagcag
     1081 ctggtcggga tcaacacgat catctacttc gcccccacca cgctcaccaa cgtcggctac
     1141 gggccgtcgg gcgccatcta cgccaacctc gtgatcggcg tgctgaacgt ggccgccacg
     1201 gtcgtcgccg tccggatcgt ggaccgcgtg ggccgcaagc cgctgctgct ggcgggcctg
     1261 gccgggatgg tcaccagcct gaccgtgctg gggctgtcga acctgctgat ggccgagccc
     1321 aactcggccg gcgacccgct ggccatcgtc acgctggcct gcctgggcgg cttcatcgtc
     1381 tccttcgccg ccacctgggg gccggtggtg tgggtcatgc tccccgaggt cctgccgctg
     1441 agcgtgcgcg gctcggccat gggcgtggcc atcttcctgc actggggggc caacttcctg
     1501 gtggcccaga cgttcccgat cctgctggac gagttcgagg ccggcccggt gttcctgggc
     1561 tacgcggtca tcggggtgat cgccgcggtg ttcgtgacca ccaaggtcca ggagaccaag
     1621 ggccgcagcc tggaggcgat cgaggaggag ctccaggacg aagcgccggc ggcggcgtag
     1681 gcggccggcg cggttcaagc gtttgggtcg ccacagcggc ccaaaagtat gaaccgacgt
     1741 ttcggcttcg gcgctcgccg tctgccgccc cggcgttggg tcacagaacg ctgacgaacg
     1801 caacagcgcc gtcacgagaa ccccgcccta cccagccgcc gttggcgccg atccgctggt
     1861 gcggccgctc gggagggaag aggcggccag ccccgcctag ccccccagcg gggtgtcctg
     1921 taggtccgcg cgcagctcgc tgagcgattc gaacaccgcc acggcgccgg cctcctcgag
     1981 ctcggcgcgc gagaagccgc cggtcatgac ggccaccgtg gggaccttgg ccttcttggc
     2041 cgcctcgatg tcccac
//
LOCUS       DASUPW010000332         2760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1464698, whole genome shotgun sequence.
ACCESSION   DASUPW010000332 DASUPW010000000
VERSION     DASUPW010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464698"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /locus_tag="VFY44_07450"
     CDS             <1..1245
                     /locus_tag="VFY44_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HEX5910313.1"
                     /translation="LRGGVAPDHHEIKRLDETFDRDVLGPRCRFLGNVEVGKDISHAD
                     LMRHYDAVVYAVGAQTDKSLGIPGEDLPGSLPATDFVGWYNGHPDRRELDPPLSGRRA
                     VIIGNGNVAADVARMLTLDPAELERTDTAEHALEALRGSGIEEVVVLGRRGPAQAAFT
                     SAELREMGHMEGVDLHVDPAAVELDPVSQEWLAEEGTFTAGKNVELLREFAAAEPKGA
                     PRRIELRFLTSPVAIHGDGKVESIEVQRNRIERDDSGALRARPADEATETIECDLVLR
                     SVGYRAVPLPDVPFDERNYVLPNEHGRVVDADSREPVPGVYAVGWIKRGPTGILGTNK
                     RDANETVGLLLEDLDGLPAPEAPGAEAIDALLAEKAPGLVTVEGWRAIDALERERGEA
                     AERPRVKLADRDALLDTARAGA"
     gene            1242..1631
                     /locus_tag="VFY44_07455"
     CDS             1242..1631
                     /locus_tag="VFY44_07455"
                     /inference="COORDINATES: protein motif:HMM:NF021510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HEX5910314.1"
                     /translation="MSELNVLGGDLEPCGTDPVTGFFRDGCCRTGPDDLGSHTICAVV
                     TEEFLDHQRGIGNDLATPMPQFRFPGLEPGDRWCVTAANWLRAHHEGSAAYVVLAATH
                     ERALDIVPLEILREHAVDVPADPGALG"
     gene            complement(1777..2424)
                     /locus_tag="VFY44_07460"
     CDS             complement(1777..2424)
                     /locus_tag="VFY44_07460"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEX5910315.1"
                     /translation="MELIPRERTELSPGAVHVPDWLDVDGQRRLVEACREWARGPAPM
                     RHTRLPSGGTMSVQTVCLGWHWLPYRYSRTADDVDGSPVTPMPGWLAQMGARAVADAY
                     GEAEERAFDAALVNFYDDAARMGLHQDKEERSDAPVVSLSIGDSCVFRFGNTRTRGKP
                     YTDVELRSGDLFVFGGPARFAYHGVPKILPGTADPACGMESGRLNLTLRVTGLEG"
     gene            complement(2415..>2760)
                     /locus_tag="VFY44_07465"
     CDS             complement(2415..>2760)
                     /locus_tag="VFY44_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910316.1"
                     /translation="PEAREAALRERPDAYLEYVAGDVGEPGLVERLVAQYDLNALVCS
                     GGISHIPVEAFAAGWAAFPAGSWLVATAREPMDEYAEGLEDVYTETFVHRLSFSGEQI
                     EYIALRGRKPWS"
BASE COUNT          372 a         1055 c          996 g          337 t
ORIGIN      
        1 ctgcgcggcg gcgtggcgcc cgaccaccac gagatcaagc gcctggacga gaccttcgac
       61 cgcgacgtcc tgggcccgcg ctgccgcttc ctgggcaacg tcgaggtggg caaggacatc
      121 tcgcacgccg acctgatgcg ccactacgac gcggtcgtct acgcggtggg cgcccagacc
      181 gacaagtcgc tgggcatccc cggcgaggac ctccccggat ccctcccggc caccgatttc
      241 gtgggctggt acaacggcca tccggaccgc cgcgagctgg acccgccgct gtccggcagg
      301 cgcgcggtga tcatcggcaa cggcaacgtg gccgcggacg tggcccggat gctgacgctg
      361 gaccccgccg agctggagcg caccgacacc gccgagcacg cgctggaggc gctgcgcggg
      421 tcgggcatcg aggaggtcgt ggtgctggga cggcgcggcc ccgcccaggc cgcgttcacc
      481 agcgccgagc tgcgcgagat gggccacatg gagggcgtcg acctgcacgt ggatcccgcc
      541 gcggtcgagc tggaccccgt ctcgcaggag tggctggccg aggagggcac cttcaccgcc
      601 ggcaagaacg tggagctgct gcgcgagttc gccgccgccg agcccaaggg cgccccgcgc
      661 cggatcgagc tgcgcttcct gacctcgccc gtggccatcc acggcgacgg caaggtggag
      721 tcgatcgagg tccagcgcaa ccggatcgag cgcgacgact ccggcgccct gcgcgcccgc
      781 cccgccgacg aggccacgga gacgatcgag tgcgacctgg tgctgcgctc ggtgggctac
      841 cgcgcggtgc cgctgcccga cgtgcccttc gacgagcgca actacgtcct gcccaacgag
      901 cacggccggg tcgtggacgc cgactcgcgc gagcccgtcc ccggcgtgta cgccgtcggc
      961 tggatcaagc gcggacccac cggcatcctg ggcaccaaca agcgcgacgc caacgagacc
     1021 gtcgggctgc tgctggagga cctggacggc ctgcccgcgc ccgaggcgcc gggcgcggag
     1081 gccatcgacg cgctgctggc cgagaaggcg cccggcctgg tgaccgtcga gggctggcgc
     1141 gccatcgacg cgctggagcg cgagcggggc gaggcggccg agcggccgcg ggtcaagctg
     1201 gccgaccggg acgcgctgct ggacaccgcc cgcgccggcg cgtgagcgag ctcaacgtcc
     1261 tgggcgggga cctcgagccc tgcgggaccg acccggtcac gggcttcttc cgtgacggct
     1321 gctgtcgcac cgggcccgac gacctgggca gccacaccat ctgcgccgtt gtcaccgagg
     1381 agttcctgga ccaccagcgc ggcatcggca acgacctggc caccccgatg ccccagttcc
     1441 gcttccccgg cctcgagccc ggcgaccgct ggtgcgtgac cgccgccaac tggctccgcg
     1501 cccaccacga gggctccgcc gcctacgtgg tgctggccgc gacccacgag cgcgcgctgg
     1561 acatcgtgcc cctggagatc ctgcgcgagc acgcggtgga cgtgccggcg gatccgggcg
     1621 cgctggggta gctacgggga cgtccggggc cgtgcacgtc ccggcccgga accgcggctg
     1681 ctcgtcctgg gctcgtgcgt gcacgtcagc gcgcagatcc cgacccgcga gccggcagcc
     1741 tgctcgtggg cccggttgtg cacgcggcgt gggggcctag ccctccagcc ccgtcacccg
     1801 cagcgtcagg ttcaaccgcc ccgactccat cccgcaggcg gggtcggcgg tgcccggcag
     1861 gatcttcggc accccgtggt aggcgaagcg ggccgggccg ccgaacacga acaggtcgcc
     1921 cgagcgcagc tcgacgtcgg tgtagggctt cccgcgcgtc cgggtgttgc cgaagcggaa
     1981 cacgcacgag tcgccgatgc tcagcgacac gaccggcgcg tcggagcgct cctccttgtc
     2041 ctggtgcagg cccatcctgg ccgcgtcgtc gtagaagttg accagcgccg cgtcgaacgc
     2101 ccgctcctcc gcctcgccgt aggcgtcggc caccgcccgg gcgcccatct gcgccagcca
     2161 ccccggcatg ggcgtgaccg gcgagccgtc gacgtcgtcg gccgtgcgcg agtagcggta
     2221 gggcagccag tgccagccca ggcacaccgt ctgcacggac atggtgccgc cgctgggcag
     2281 cctggtgtgc cgcatcgggg cggggccacg cgcccactcg cggcaggcct cgaccagccg
     2341 ccgctgcccg tccacgtcca gccagtccgg cacgtggacc gcgccgggcg agagctcggt
     2401 ccgctcgcgc gggatcagct ccacggcttg cggccgcgga gcgcgatgta ctcgatctgc
     2461 tcgccgctga acgacagccg gtggacgaag gtctcggtgt acacgtcctc caggccctcg
     2521 gcgtactcgt ccatgggctc gcgcgcggtg gccaccagcc acgacccggc cgggaacgcc
     2581 gcccagcccg cggcgaacgc ctcgacgggg atgtgcgaga tgccccccga gcacaccagg
     2641 gcgttcaggt cgtactgcgc gaccagccgt tccaccaggc cgggctcgcc gacgtcgccc
     2701 gccacgtact ccaggtaggc gtccgggcgc tcgcgcagcg ccgcctcgcg ggcctcgggg
//
LOCUS       DASUPW010000333         3456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1741919, whole genome shotgun sequence.
ACCESSION   DASUPW010000333 DASUPW010000000
VERSION     DASUPW010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1741919"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VFY44_07470"
     CDS             <1..407
                     /locus_tag="VFY44_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910317.1"
                     /translation="WGQPDPPPPAAPPVARAAAPAPFLFRSSFVSSRVGARHLKRTIH
                     RVTVSGRFRGRGCGGRVALTYRLRGMRTVTRSLRVGRDCRYRGVVDLRGPARMRRGDR
                     LKIGQRFTGNGATPAGTGRTLSVKLSRPPRRA"
     gene            complement(382..828)
                     /locus_tag="VFY44_07475"
     CDS             complement(382..828)
                     /locus_tag="VFY44_07475"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5910318.1"
                     /translation="MAIEGSASIEIDAPIEEVFEVAADVEESPRWQPDIKKAEVKERD
                     ADGRQVLVRTETDAKVRTLGSNLRFTYDAPNGLTWRQEKGDLKSVEGSWTFDDLGDGR
                     TRATYWMEVDLGRMLGAVIRGPLVDAMRKQLVESMPEKLKRDVEGG"
     gene            complement(861..1502)
                     /locus_tag="VFY44_07480"
     CDS             complement(861..1502)
                     /locus_tag="VFY44_07480"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5910319.1"
                     /translation="MASPTAPERNPRGQGDLLRDRLVDAAIAMLDEGTDPAALSIRAV
                     TKRAGVSPTAFYLHFAHRDELVAAMIDRCFTEFRDHVRRGAAGAGGPAERLNRSGMAY
                     AEFARRWPARYALNFVFMKPAEGEGEAPEKPVAANDSFNDLVGAVLDYLPEDDPRRDE
                     VDLLARGIWSGLHGYVSLSRTRTGMGWPGEDEFIPRLSRAWLGEPRPATDQSH"
     gene            1588..>3456
                     /locus_tag="VFY44_07485"
     CDS             1588..>3456
                     /locus_tag="VFY44_07485"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5910320.1"
                     /translation="MLSSLANRISARPRRVLVIAALFTVFAAAVGGPVAGMLDAGGGF
                     TDRDSDSAQAVERLDAATKERASGSAILLVRPGGSARSEAARAEVTALGRQAAAIPGV
                     SSVASATTTRDPRFVSRDGRSTYLAVSLKASADEDSVRTALEQRFEARKDVTLGGQLF
                     ANDQLGAQISEDLGRAEMLAFPLLILLSIFFFRGARAAVLPLVIGATTVLTTFLMLRV
                     VDGFHDLSIFCLNLVIGMGLGLAIDYTLFLLHRYREELERSGPGAKAVRDTMATAGRT
                     VVFSALTVAIALATLLLFPLNFLQSMAIGGAGVALVAAAASCLIAPAFFALWNVKLKA
                     KDRAKRADARWYGVAQGVMRRPGAIAAVTALVMVMLALPALRATWTPVDIQTLPEGLS
                     ARTVGDELARDFPGQDSSPVQLAVEAGRGEGAQVRALARQVAQEPAIASVGEPRRLDG
                     TTWQIEAIARGDAAGVPAREAVESIRGYDTELSVLVGGEAADFIDQQSAIGSALVPAM
                     VMLALLTFAVLWLMTGSVVLPVKALIMNVLTVGATLGVLTLIFQDGRLEGLLGYSSNG
                     GIEPTDFLVTATLVFALSTDYGVFLLGRIKEARDRGLPDREAVAAGLSVTGSVVTAA"
BASE COUNT          418 a         1268 c         1247 g          523 t
ORIGIN      
        1 agtggggtca gcccgatccg ccgccgcccg cggcgcctcc ggtggcccgc gccgcggcgc
       61 cggcgccgtt cctgttccgc tcgtccttcg tcagctcacg cgtgggcgcg cgccacctca
      121 agcggaccat ccaccgcgtc accgtcagcg ggcgcttccg cgggcgcggc tgcggcggcc
      181 gcgtggcgct gacctaccgc ctgcgcggca tgcggaccgt cacgcgcagc ctgcgcgtgg
      241 ggcgcgactg ccgctaccgc ggcgtggtgg acctgcgcgg gccggcgcgg atgcgcaggg
      301 gggaccggct gaagatcggc cagcgcttca ccgggaacgg cgcgacgccc gccgggacgg
      361 gacggacgct gagcgtgaag ctcagccgcc ctccacgtcg cgcttgagct tctcgggcat
      421 cgactccacc agctgcttgc gcatggcgtc caccagcggg ccgcggatca cggcgccgag
      481 catccggccc aggtcgacct ccatccagta ggtggcgcgg gtccgcccgt cgcccaggtc
      541 gtcgaaggtc cacgagccct ccaccgactt caggtcgccc ttctcctggc gccaggtcag
      601 cccgttcggg gcgtcgtagg tgaagcgcag gttcgacccc agcgtccgga ccttggcgtc
      661 ggtctcggtg cgcaccagca cctggcggcc gtcggcgtcg cgctccttga cctcggcctt
      721 cttgatgtcg ggctgccacc ggggcgactc ctccacgtcg gcggccacct cgaagacctc
      781 ctcgatcggc gcgtcgatct cgatgctggc ggatccctcg attgccattc gcttcctccc
      841 cggtcgctgg tggcgccagc ctagtgcgac tggtcggtcg cggggcgggg ctcgcccagc
      901 catgcgcgcg acaggcgcgg gatgaactcg tcctcgccgg gccagcccat gcccgtgcgc
      961 gtccggctca ggctgacgta gccgtgcagg cccgaccaga tgccccgggc cagcaggtcg
     1021 acctcgtcgc ggcggggatc gtcctccggc aggtagtcca ggaccgctcc caccaggtcg
     1081 ttgaacgagt cgttggcggc caccggcttc tccggcgcct cgccctcgcc ctcggccggc
     1141 ttcatgaaca cgaagttcag cgcgtagcgc gccggccagc ggcgcgcgaa ctcggcgtag
     1201 gccatgccgg agcggttgag ccgttcggcc ggaccgccgg ccccggcggc gccccggcgc
     1261 acgtggtccc ggaactcggt gaagcagcga tcgatcatcg cggcaaccaa ttcgtcccgg
     1321 tgtgcgaagt gaaggtagaa ggccgtcggc gacacgcccg cccgcttggt caccgcgcgg
     1381 atcgagaggg cggcggggtc ggtgccctcg tcgagcatcg cgatcgccgc gtccacgagg
     1441 cggtcgcgca ggaggtcgcc ctgtccgcgg gggttgcgct cgggggcggt gggagaggcc
     1501 atggagccca atctacaccg tcacttgaca tctgtacata tacatctgta actttacgac
     1561 cgtcaaccaa gcaccgaggt cgccaccatg ctctcttcac tcgccaaccg gatctccgcc
     1621 cgcccccgcc gcgtcctcgt gatcgcggcg ctgttcaccg tcttcgcggc tgccgtgggc
     1681 gggcccgtgg ccggcatgct ggacgcgggc ggcggcttca ccgaccgcga ttccgactcc
     1741 gcccaggcgg tcgagcgcct ggatgcggcg accaaggagc gcgcgtccgg cagcgcgatc
     1801 ctgctggtgc gccccggcgg ctccgcccgc tcggaggccg cgcgggccga ggtcacggcg
     1861 ctgggccgcc aggccgccgc gatcccgggg gtctcctccg tggcctcggc aaccaccacg
     1921 cgcgacccgc ggttcgtctc gcgcgacggc cggtcgacgt acctggcggt gtcgctgaag
     1981 gcgtccgccg acgaggactc ggtgcggacc gcgctggagc agcggttcga ggcccgcaag
     2041 gacgtcacgc tgggcgggca gctgttcgcc aacgaccagc tgggagcgca gatctccgag
     2101 gacctgggcc gtgccgagat gctggccttc ccgctgctga tcctgctgtc gatcttcttc
     2161 ttccgcggcg cgcgggcagc ggtgctgccg ctggtcatcg gcgccacgac ggtcctgacc
     2221 acgttcctga tgctgcgcgt ggtcgacggg ttccacgacc tgtcgatctt ctgcctgaac
     2281 ctggtcatcg gcatgggcct gggcctggcc atcgactaca cgctgttcct gctgcaccgc
     2341 taccgcgagg agctcgagcg cagcgggccg ggcgccaagg ccgtgcgcga caccatggcc
     2401 accgccgggc gcacggtggt gttcagcgcc ctgaccgtgg ccatcgcgct ggccacgctg
     2461 ctgctgttcc cgctgaactt cctgcagtcg atggccatcg gtggcgccgg ggtggcgctg
     2521 gtggcggcgg ccgcctcgtg cctgatcgcg ccggcgttct tcgcgctctg gaacgtcaag
     2581 ctcaaggcga aggaccgtgc caagcgcgcc gacgcgcgct ggtacggcgt ggcccagggc
     2641 gtgatgcgcc gccccggcgc gatcgcggcg gtcaccgccc tggtcatggt catgctggcg
     2701 ctgcccgcgc tccgcgccac gtggaccccc gtcgacatcc agacgctgcc ggagggcctc
     2761 agcgcccgga cggtcggtga cgagctggcg cgcgacttcc ccggccagga cagcagcccg
     2821 gtccagctgg ccgtcgaggc cggcaggggc gagggcgcgc aggtgcgggc gctggcccgt
     2881 caggtcgcgc aggagccggc catcgcctcc gtgggcgagc cgcgcaggct ggacggcacc
     2941 acctggcaga tcgaggcgat cgcccgtggc gacgcggccg gcgtgccggc gcgcgaggcg
     3001 gtggagtcca tccgcggcta cgacaccgag ctctcggtcc tggtgggtgg cgaggccgcc
     3061 gacttcatcg accagcagag cgcgatcggg tcggcgctgg tgcccgcgat ggtcatgctg
     3121 gccctgctga ccttcgccgt cctgtggctg atgaccgggt cggtggtgct gccggtgaag
     3181 gcgctgatca tgaacgtcct gacggtgggc gccacgctgg gggtgctgac gctgatcttc
     3241 caggacggcc gcctcgaggg gttgctgggc tacagcagca acggcggcat cgagcccacc
     3301 gacttcctgg tcacggccac gctggtgttc gcgctctcca ccgactacgg cgtgttcctg
     3361 ctgggccgga tcaaggaggc ccgcgaccgc ggcctgcccg accgcgaggc ggtggccgcc
     3421 ggactgtcgg tcaccggcag cgtggtcacc gcggcc
//
LOCUS       DASUPW010000334         4720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1325351, whole genome shotgun sequence.
ACCESSION   DASUPW010000334 DASUPW010000000
VERSION     DASUPW010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325351"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VFY44_07490"
     CDS             complement(<1..291)
                     /locus_tag="VFY44_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910321.1"
                     /translation="MSSQTRNERGDASSLDLRTLAIASVASAVAAIVVSRFWTSGTPL
                     AAAITPVLVTLFKEALDRPTAKIAEKVTVSSKALPQTRIREPAASHVGRDAQL"
     gene            348..1709
                     /locus_tag="VFY44_07495"
     CDS             348..1709
                     /locus_tag="VFY44_07495"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HEX5910322.1"
                     /translation="MSDASQTVDVAVVGAGLAGLTAARDLIAAGHSVAVVEARDRVGG
                     RTLNEPLGDGKVVEVGGQWIGPTQDRMAALAREMGVDTFPTHAQGENLNEWRGTMRRY
                     RGSIPKINPAVLVDVEQAQQRLNRMAKSVPLDRPWAADKAARWDSTTVRTWMERSMAT
                     RGGKDLLALGVQSVWATEPEDFSLLHMLFYIHSGGSLAMLFDTEGGAQESRFVGGSQL
                     VSQRLADSLGDDVVHLGAPVRRIEHGGDGVTVVADGLTVRAQRVVVSLSPPLAGRIAY
                     DPPLPGYRDQLTQRTPMGVVAKTMAVYDEPFWRADGLSGQATSDVGPVKLTFDNSPPN
                     GSPGVLLGFVEGDRARGFALLSADERRRAVVDTFARLFGPRAAKPERYIERLWSEEEY
                     TRGCYGAHMPTGAWVAYGKALREPIGPIHWAGSEVALIWTGYMDGAVRSGESAAREVV
                     SAL"
     gene            complement(1696..2124)
                     /locus_tag="VFY44_07500"
     CDS             complement(1696..2124)
                     /locus_tag="VFY44_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910323.1"
                     /translation="MSALLLANAAATLFMCGLIWFVQVVHYPLLARVGRAELPAVHDR
                     HSRSVTWLVLPPMSVELVTAGALALWPPDGAPAALTWIGLALAAAVWAVTGLVQVPAH
                     ARVGREGATAVPALVAGNWVRTALWTARAGVVLALLATAH"
     gene            2144..3589
                     /locus_tag="VFY44_07505"
     CDS             2144..3589
                     /locus_tag="VFY44_07505"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5910324.1"
                     /translation="MPDVVVIGAGHNGLTAAAYLARAGLSVEVLERRDIAGGACITEE
                     LWPGVRASPGAYTLSLLRPEIVSDLDLAAHGLQVSVHEPYLFAPFPDGRKVVTWSSRE
                     RTHAQLERDWSRADADGYAVFAQRWEQAAARARPLMSEPPDRERWLEAVGPGILDGPV
                     AAELEDIPSEQVRVPFAVQGLIGTLAGPEDPGTAFVGFYHDLGQAAGVDGAWGYARGG
                     MGAVSGALRAAAEAAGARIRLESPVERVLIEDGAAVGVVLEGGEEVRAARVLSNADPL
                     RTSALAGVDAPEGWRQAGPVVKVMVLLDGLPDFTAWPGEEPWQGAIDIGFTMDDLTSA
                     ADDARAGRPAARPWIEAACQTASDDSLAPEGKHVLSMFCQCFPADVDAEAAADVAVAR
                     FAEVCPELPDRIIGRLALGPRQLEERFGITGGHIFHGEMLPGQLLEQRPDRRRFGGIE
                     NLYLAGSGAHPGGAVTGAPGLLAARAVLEDR"
     gene            3589..4527
                     /locus_tag="VFY44_07510"
     CDS             3589..4527
                     /locus_tag="VFY44_07510"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX5910325.1"
                     /translation="MARVYVTRDLPGGALDRLREQHDVDLWDGDDQPPADELARRVAE
                     AEGLLSMLGDRVDAELLDGAPKLRAVANYAVGVDNVDVEAATERGIPVGHTPDVLTGA
                     TADLTLALMLAAMRRLPEAEAQVREGRWTSWAPDGLLGRDLGRSTVLIVGRGRIGQAV
                     AERVGGFGAEVVTAGRGDQLEPLLEQADVVTLHCPLTEETRGLIGEAELRAMGPEAYL
                     VNTARGPVVDTGALVRALTEGWIAGAGLDVTDPEPLPGDHPLLEAPNLAIAPHLGSAT
                     HYTRAAMADLAVDNLLAALDGQRMPHTVNPEAWTDG"
BASE COUNT          594 a         1709 c         1850 g          567 t
ORIGIN      
        1 cagctgggcg tcgcggccca cgtggctggc ggccggctcg cggatccggg tctgcggcag
       61 cgccttggac gacaccgtca ccttctcggc gatcttggcc gtggggcggt ccagcgcctc
      121 cttgaacagc gtgaccagga caggggtgat ggccgccgcg aggggcgtgc ccgaggtcca
      181 gaagcgggaa accacgatcg ccgccaccgc tgatgcgacg ctggcgatgg ccagcgtgcg
      241 gaggtccagg ctgctcgcgt ccccgcgctc gttgcgcgtc tgcgaggaca tgagataggc
      301 agggtagcga gcttcaacgc gcccgtctgg acgggtaggt tggccccatg agcgatgcgt
      361 cacagaccgt cgacgtggcc gtggtgggtg ccggcctggc ggggctgacg gccgcgcggg
      421 acctgatcgc cgcgggccat tcggtggcgg tggtcgaggc ccgcgaccgg gtcggcggcc
      481 gcacgctgaa cgagccgctg ggcgacggca aggtggtcga ggtgggcggc cagtggatcg
      541 gcccgaccca ggaccggatg gcggcgctgg cccgcgagat gggcgtcgac accttcccca
      601 cgcacgcgca gggcgagaac ctgaacgagt ggcgcggcac gatgcgccgc taccggggct
      661 cgatacccaa gatcaacccc gcggtgctgg tggacgtcga gcaggcccag cagcgcctga
      721 accggatggc gaagtcggtg ccgctggacc gcccgtgggc ggcggacaag gcggccaggt
      781 gggactccac gacggtgcgc acctggatgg agcgctcgat ggccacccgc ggcggcaagg
      841 acctgctggc gctgggcgtg cagtcggtct gggccaccga gcccgaggac ttctcgctgc
      901 tgcacatgct gttctacatc cactccggcg gcagcctggc catgctcttc gacaccgagg
      961 gcggcgcgca ggagagccgc ttcgtgggtg ggtcgcagct ggtgtcgcag cggctggcgg
     1021 acagcctggg cgacgacgtg gtgcacctgg gcgcaccggt gcgccggatc gagcacgggg
     1081 gcgacggcgt gacggtggtc gccgacggcc tgacggtgcg cgcgcagcgc gtggtggtgt
     1141 ccctgtcgcc cccgctggcc ggccggatcg cctacgaccc gcccctgccc gggtaccgcg
     1201 accagctgac ccagcgcacg cccatgggcg tggtggccaa gaccatggcc gtgtacgacg
     1261 agccgttctg gcgcgccgac gggctcagcg gccaggccac cagcgacgtc ggaccggtca
     1321 agctgacctt cgacaactcg ccgccaaacg gctcgcccgg cgtgctgctg ggcttcgtcg
     1381 agggcgaccg cgcgcgcggg ttcgcgctcc tgtccgccga cgagcgccgg cgggccgtcg
     1441 tggacacctt cgcgcgcctg ttcgggcccc gggccgccaa gcccgagcgc tacatcgagc
     1501 ggctctggtc cgaggaggag tacacgcgcg gctgttacgg cgcccacatg cccaccgggg
     1561 cctgggtggc ctacggcaag gccctgcgcg agccgatcgg cccgatccac tgggcgggca
     1621 gcgaggtggc gctgatctgg acgggctaca tggacggcgc cgtgcgctcg ggcgagagcg
     1681 ccgcccgcga ggtcgtcagt gcgctgtagc cagcagcgcc aggaccacac cggcacgggc
     1741 cgtccagagc gcggtccgca cccagttgcc cgccaccagc gcgggcaccg cggtcgcgcc
     1801 ctcgcgcccc acgcgggcgt gggcggggac ctggaccagc cccgtgaccg cccacaccgc
     1861 ggccgccagc gccaggccga tccaggtcag tgcggcgggc gcgccgtccg gcggccacag
     1921 cgccagggcg ccggcggtga ccagctcgac cgacatcggc ggcagcacga gccacgtgac
     1981 cgagcgcgag tggcggtcgt gcaccgccgg cagctccgcg cggcccacgc gcgccagcag
     2041 ggggtagtgg accacctgca cgaaccagat cagtccgcac atgaacaggg tggcggcggc
     2101 gttggccagc agcagggcgg acatctcggc gggggatacg cgcgtgcctg acgtcgtggt
     2161 catcggcgcc ggccacaacg gcctgaccgc cgcggcctac ctggcgcgcg ccggcctgtc
     2221 ggtcgaggtc ctggagcggc gcgacatcgc cggcggggcg tgcatcaccg aagagctgtg
     2281 gcccggcgtc cgcgcgtccc ccggcgccta caccctgtcg ctgctgcggc cggagatcgt
     2341 cagcgacctg gatctggccg cccacggcct ccaggtctcg gtgcacgagc cctacctgtt
     2401 cgcgccgttc ccggacgggc gcaaggtcgt gacctggtcc tcgcgcgagc gcacccacgc
     2461 ccagctggag cgcgactggt cacgtgccga cgccgacggc tacgcggtgt tcgcccagcg
     2521 ctgggagcag gcggcggcgc gggcgcggcc gctcatgtcc gagccgcccg accgcgagcg
     2581 ctggctcgag gccgtcggcc cgggcatcct ggacgggccg gtggccgccg agctggagga
     2641 catcccctcc gagcaggtgc gcgttccatt cgcggtgcag ggcctgatcg gcacgctggc
     2701 cggccccgag gacccgggca ccgccttcgt cggcttctac cacgacctcg gtcaggcggc
     2761 cggcgtcgac ggggcctggg gctacgcccg cggcggcatg ggggcggtgt cgggcgcgct
     2821 ccgcgcggcg gccgaggccg cgggcgcgcg catccggctg gagagcccgg tcgagcgcgt
     2881 gctgatcgag gacggcgccg cggtcggcgt ggtcctggag ggcggcgagg aggtgcgcgc
     2941 cgcgcgcgtc ctgtccaacg ccgacccgct gcggacctcc gcgctggccg gcgtcgacgc
     3001 gccggagggc tggcgccagg ccgggccggt ggtcaaggtc atggtgctgc tggacgggct
     3061 gcccgacttc accgcctggc cgggcgagga gccgtggcag ggcgccatcg acatcggctt
     3121 caccatggac gacctgacca gcgcggccga cgacgcgcgc gccgggcgcc ccgccgcgcg
     3181 gccgtggatc gaggccgcct gccagaccgc ctccgacgac tcgctggccc ccgagggcaa
     3241 gcacgtgctg tccatgttct gccagtgctt cccggccgac gtggacgccg aggcggcggc
     3301 cgacgtggcc gtggcccggt tcgccgaggt gtgcccggag ctgccggacc ggatcatcgg
     3361 gcgcctggcg ctgggcccga ggcagctgga ggagcggttc ggcatcaccg gcggccacat
     3421 cttccacggc gagatgctcc ctggccagct cctggagcag cgccccgacc ggcgccgctt
     3481 cggcggcatc gagaacctgt acctggccgg ctcgggagcg cacccgggcg gcgcggtcac
     3541 cggcgcgccg ggcctgctgg cggcacgcgc ggtgctcgag gaccgctgat ggcccgcgtc
     3601 tacgtgacgc gtgacctgcc cggcggggcg ctggaccgcc tgcgcgagca acacgacgtc
     3661 gacctgtggg acggcgacga ccagccgcct gcggacgagc tggcgcgcag ggtggccgag
     3721 gccgaggggc tgctgagcat gctcggcgac cgggtcgacg ccgagctgct ggacggggcg
     3781 ccgaagctgc gggcggtggc caactacgcc gtcggcgtgg acaacgtgga cgtggaggcg
     3841 gccaccgagc ggggcatccc cgtgggccac acgcccgacg tgctgaccgg ggcgaccgcc
     3901 gatctgaccc tggcgctgat gctggccgcc atgcgccgcc tgcccgaggc cgaggcgcag
     3961 gtgcgcgagg ggcgctggac ctcgtgggcc ccggacggcc tgctgggtcg cgacctcggc
     4021 cggtccacgg tgctgatcgt gggccgcggc cggatcgggc aggccgtggc cgagcgggtg
     4081 ggcgggttcg gggcagaggt cgtcaccgcg gggcgcggcg accagctgga gccgctgctg
     4141 gaacaggccg acgtcgtgac gctgcactgc ccgctgaccg aggagacgcg cggcctgatc
     4201 ggcgaggccg agctgcgggc gatggggccc gaggcctacc tggtcaacac cgcacgcggg
     4261 ccggtggtcg acaccggcgc gctggtccgc gccctgaccg aggggtggat cgccggcgcc
     4321 ggcctggacg tgacggatcc cgagccgctg ccaggcgacc acccgctgct ggaggcgccc
     4381 aatctggcca tcgccccgca cctgggctcg gccacgcact acacgcgcgc ggccatggcc
     4441 gacctggccg tggacaatct gctggccgcg ctggacgggc agcgaatgcc gcacacggtg
     4501 aacccggagg cctggacgga cggctgaccc gggcagggga cggctgcggc ggcgcgaata
     4561 gcgcagcgat gagccgccga accgtgtcga cctgcgtcgt gtgcgcgttc gccctcgccc
     4621 cggccgcctt cgcggccgac gtccccggtg gcggccaggc cgcctccacc ggcggtgcgg
     4681 tggccccggc cccggccccg caggcgcccg cgctggcgcc
//
LOCUS       DASUPW010000335         4034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1326088, whole genome shotgun sequence.
ACCESSION   DASUPW010000335 DASUPW010000000
VERSION     DASUPW010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326088"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..888)
                     /gene="pdxS"
                     /locus_tag="VFY44_07515"
     CDS             complement(1..888)
                     /gene="pdxS"
                     /locus_tag="VFY44_07515"
                     /inference="COORDINATES: protein motif:HMM:NF003215.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX5910326.1"
                     /translation="MREESTFRTKAGLATMLKGGVIMDVVTPEQARVAEAAGAAAVMA
                     LERVPADIRADGGVARMSDPTMIEGIQQAVTIPVMAKARIGHFVEAQVLEALEVDYVD
                     ESEVLTPADEAHHIDKWAFKVPFVCGATNLGEALRRIAEGAAMIRSKGEAGTGNVVEA
                     VRHMRKIGGEIRRLTSLDEVELATAAKDLQAPLDLVREVAEKGKLPVVMFSAGGVATP
                     ADAALMMRLGAEGVFVGSGIFKSEDPERTAAAVVEATTHFEDPARIASASRDLGAPMT
                     GIGMDVIDEHGGPLAQRGW"
     gene            complement(947..1702)
                     /locus_tag="VFY44_07520"
     CDS             complement(947..1702)
                     /locus_tag="VFY44_07520"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5910327.1"
                     /translation="MPGSDSDAGSKPVALVTGAAGGIGRAVSERLRSDGYGVLDVDLE
                     AGDDGFAVDLTTREGNKGAVDEALERFGRLDVIVANAGVQHVSAVAEFPEDKWDLLIS
                     LMLTSPFLLARYGWEALGKAPDGGRFIAIASAHALAASPYKSAYVSAKHGVLGLVKTL
                     ALEGAEQGTLATAVCPAFVRTPLVEKQIADQAKAHGISEDEVLEQVILAPHAVKRLIE
                     PSEVADTVAFLAGPGGATFTGVPVTMDLGWTAR"
     gene            complement(1811..2665)
                     /locus_tag="VFY44_07525"
     CDS             complement(1811..2665)
                     /locus_tag="VFY44_07525"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX5910328.1"
                     /translation="MKSTGTAALTLSAEIRSRFDEFSRSQKDVGQYIVDHLDEASFHT
                     AEELARRANTSSSTVVRFAQALGFEGFPELQASAREEYRRIRDGEDGGVVDVANPPLF
                     PIDQTEFEASLAADHTNVEDTARKVDREEVEACIDLISHAERIVLCGTDQMAFFASYL
                     RHLLMLLDLRCEVVASPSQEGLARLGRIDENSVLVGFSAGRPHPLVVRAMKLARNRRA
                     ATISVSDATLSEVAKLADHKLFYSSTSPAYVRSHAALLSLVQALAYGIYAQDEPAYAD
                     RIKAFKLK"
     gene            complement(2711..3430)
                     /locus_tag="VFY44_07530"
     CDS             complement(2711..3430)
                     /locus_tag="VFY44_07530"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX5910329.1"
                     /translation="MAGQTPDVLILCGGRGTRLQEHNQTLPKPLVEIGGWPIVWHVVR
                     IYAAQGFRRFVLLTGHKSDEVEQFTAGATWPEGVDVECVFTGEDTPTGGRVHRARERL
                     RDGTFALTYADGVADMDLAAELAHHRAHGDLATMAVVRPELPFGVTEIGGDDRVSGFH
                     EKPRSDHWINGGFFFLEPGVLDYLDDASVFEREPLEGLATDGKLHVWRHTGFWECMDT
                     YKDAVTLNDLWAGRAPWKVWD"
     gene            3454..>4034
                     /locus_tag="VFY44_07535"
     CDS             3454..>4034
                     /locus_tag="VFY44_07535"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX5910330.1"
                     /translation="MAVADTLFIAAEQVDGVLGRPDQDQLLPAREVQALSFAVHIPLV
                     CFGIAFPAMVLFAEGLWLRTGNPVYKALAKRWSKVTLVLFAIGVVTGTILSFEFGLLW
                     PNFMATFGEVFGVGFTLEGVSFFVEAIFIAIYVYGWDKLPPRTHFLVGIPVAIAGVTG
                     SMLVIAVNGWMNDPGGYDVVNGQIENVRPFAAL"
BASE COUNT          550 a         1387 c         1460 g          637 t
ORIGIN      
        1 tcaccagccg cgctgggcca gcggtccgcc gtgctcgtcg atgacgtcca tgccgatgcc
       61 ggtcatgggc gccccgaggt cgcgggaggc ggaggcgatg cgggcgggat cctcgaagtg
      121 ggtcgtggcc tcgaccaccg cggcggcggt gcgctcgggg tcctcggact tgaagatgcc
      181 ggagcccacg aacacgccct cggcgcccag gcgcatcatc agcgcggcgt ccgccggggt
      241 ggccacgcca ccggcggaga acatgaccac gggaagcttg cccttctcgg cgacctcgcg
      301 gaccaggtcc agcggggcct gcaggtcctt ggcggcggtg gccagctcga cctcgtccag
      361 gctggtcagg cggcggatct cgccgccgat cttgcgcatg tggcggacgg cctcgaccac
      421 gttgccggtg ccggcctcgc ccttggagcg gatcatcgcc gcgccctcgg cgatccggcg
      481 cagtgcctcg cccaggttgg tggcgccgca gacgaagggc accttgaacg cccacttgtc
      541 gatgtggtgg gcctcgtccg cgggcgtcag cacctcggac tcgtcgacgt agtcgacctc
      601 gagcgcctcc agcacctggg cctcgacgaa gtgcccgatc cgggccttgg ccatcacggg
      661 gatggtcacg gcctgctgga tgccctcgat catcgtggga tcggacatgc gggccacgcc
      721 gccgtcggcg cggatgtccg cgggcacgcg ctccagcgcc atgaccgcgg cggcgccggc
      781 cgcctcggcc acccgggcct gctcgggggt gaccacgtcc atgatcacgc cgcccttgag
      841 catcgtggcc aggccggcct tggtgcggaa ggtggactct tctctcacgc gtgtgatgct
      901 actcgtggac cgccgggcgg tcgatgtcac acaagccgcg ggtgccctac cgggcggtcc
      961 agcccaggtc catcgtcacc ggcacgccgg tgaaggtggc cccgccgggg cccgccagga
     1021 aggccaccgt gtcggccacc tcggagggct cgatcaggcg cttgaccgcg tgcggggcca
     1081 ggatcacctg ctccagcacc tcgtcctcgg agatgccgtg cgccttggcc tggtcggcga
     1141 tctgcttctc gaccagcggg gtgcggacga acgcggggca caccgcggtg gccagcgtgc
     1201 cctgttcggc gccctccagg gccagcgtct tgaccaggcc caacacgccg tgcttggcgg
     1261 acacgtaggc ggacttgtag ggcgatgcgg ccagggcatg ggccgaggcg atggcgatga
     1321 agcgcccccc gtcgggcgcc tttcccagcg cctcccagcc gtagcgggcc agcaggaacg
     1381 ggctggtgag catgagtgag atgagcaggt cccacttgtc ctcggggaac tcggcgaccg
     1441 cggacacgtg ctgcacgccg gcgttggcca cgatcacgtc gaggcggccg aaccgctcga
     1501 gggcctcgtc gacggcgccc ttgttgccct cccgtgtggt caggtcaacg gcgaagccgt
     1561 cgtccccggc ctcgaggtcg acgtccagaa cgccgtaacc gtcagaccgg aggcgctcgg
     1621 agaccgcccg gccgatgccg ccggcggcgc cggtcaccag agcgaccggc ttcgaccccg
     1681 cgtccgagtc cgatccgggc ataggcccga gatgcccgct gggggttttc agaaactcgc
     1741 ggtcatgggg tgaccgccgt tgccacccgg tggcaccggt tttctgaaac cgcccagcgc
     1801 gggttccgga ctacttgagc ttgaacgcct tgatgcggtc ggcgtaggcc ggctcgtcct
     1861 gggcgtagat gccgtaggcg agcgcctgga ccagcgacag cagcgccgcg tgcgagcgca
     1921 cgtaggcagg gctggtggac gagtagaaga gcttgtggtc ggccagcttg gccacctcgg
     1981 acagggtggc gtcggagacc gagatggtgg ccgcgcggcg gttgcgggcc agcttcatgg
     2041 cccggaccac cagcgggtgc gggcggccgg cggagaagcc gacgagcacc gagttctcgt
     2101 cgatccggcc caggcgggcc agcccctcct gggagggcga ggcgacgacc tcgcagcgca
     2161 ggtcgagcag catcagcagg tggcgcaggt agctggcgaa gaaggccatc tggtcggtgc
     2221 cgcacagcac gatccgctcg gcgtgggaga tcaggtcgat gcaggcctcg acctcctcgc
     2281 ggtcgacctt gcgcgcggtg tcctcgacgt tggtgtggtc cgcggccagg gaggcctcga
     2341 actcggtctg gtcgatgggg aacagcggcg ggttggccac atcgaccact cccccgtcct
     2401 caccgtcacg gatgcggcgg tattcctcgc gcgcggaggc ctggagctcg gggaagccct
     2461 cgaagcccag cgcctgggcg aagcgcacga cggtcgagct ggaggtgttg gcccggcggg
     2521 ccagctcctc ggcggtgtgg aaggaggcct cgtccaggtg gtcgacgatg tactgcccga
     2581 cgtccttctg ggagcgggag aactcgtcga accgggagcg gatctcggcg gacagggtga
     2641 gggcagcggt tccggtcgac ttcacgcatc ggtgtttcgc ccgcgccggt cgagttcctt
     2701 ccgggagtcc tcagtcccac accttccacg gggcccggcc ggcccacagg tcgttcagcg
     2761 tgaccgcgtc cttataggtg tccatgcact cccagaagcc ggtgtgcctc cacacgtgca
     2821 gcttgccgtc ggtggccagg ccctcgagcg gctcgcgctc gaacacgctc gcgtcgtcca
     2881 ggtagtccag gacgccgggc tcgaggaaga agaagccgcc gttgatccag tggtccgagc
     2941 gcggcttctc gtggaagccc gacacgcggt cgtcgccccc gatctcggtc acgccgaagg
     3001 gcagctcggg gcggacgacc gccatcgtgg ccaggtcgcc gtgcgcgcgg tggtgggcca
     3061 gctcggccgc caggtccatg tcggcgaccc cgtcggcgta ggtcagggcg aacgtcccgt
     3121 cccgcaggcg ctcgcgggcc cggtgcacgc gtccgccggt cggggtgtcc tcgccggtga
     3181 acacgcactc gacgtcgacc ccctcgggcc aggtggcgcc ggcggtgaac tgctcgacct
     3241 cgtcgctctt gtggcccgtc agcagcacga agcggcggaa gccctgtgcc gcgtagatgc
     3301 gcaccacgtg ccacacgatc ggccatccgc cgatctcgac cagcggcttg ggcagcgtct
     3361 ggttgtgctc ctgcagccgc gtgccgcggc cgccgcagag gatcaggaca tcgggggttt
     3421 ggcccgccac ggcgcagacc ttatcctcgc cgcgtggccg tagccgacac cctgttcatc
     3481 gccgcggagc aggtggatgg cgtgctcggc cgccccgacc aggatcagct cctgcccgcg
     3541 cgcgaggtgc aggcgctgtc gttcgccgtg cacatcccgc tggtctgctt cggcatcgcc
     3601 ttccccgcga tggtgctgtt cgccgagggc ctgtggctgc gcaccggcaa ccccgtctac
     3661 aaggcgctgg ccaagcgctg gtcgaaggtc acgctggtgc tgtttgccat cggcgtggtc
     3721 accggcacga tcctgagctt cgagttcggg ctgctgtggc cgaacttcat ggccaccttc
     3781 ggcgaggtct tcggcgtggg cttcacgctc gagggggtct cgttcttcgt ggaggcgatc
     3841 ttcatcgcca tctacgtgta cggctgggac aagctgccgc cgcggacgca cttcctggtc
     3901 gggatcccgg tggccatcgc gggcgtgacc gggtcgatgc tggtgatcgc cgtgaacggc
     3961 tggatgaacg atcccggtgg ctacgacgtg gtcaacggcc agatcgagaa cgtgcgcccg
     4021 ttcgccgcgc tgtt
//
LOCUS       DASUPW010000336         4518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_219738, whole genome shotgun sequence.
ACCESSION   DASUPW010000336 DASUPW010000000
VERSION     DASUPW010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219738"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VFY44_07540"
     CDS             complement(<1..774)
                     /locus_tag="VFY44_07540"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HEX5910331.1"
                     /translation="MAERGAPPKVPRVQRRVLRFISAFTSPLLPDDYLELINPLWSTR
                     ELRGKVVDVKEETPDAATVVIQPGYEWEGHEPGQYLRIGVDLNGRRHWRAYSLTSDPK
                     REDGCISITVKNVDEGVVSPYLVRKGPHGAIVGLGGVEGDFLLPDPVPEKILFISAGS
                     GITPIMSMLRSLDHEGDMPDTMHIHSAGTPEEVIFGDEMRALADKHEKFVLHEQHTAT
                     DVGRFKPADLDELCPDWKERETYISGPPDLLEALEEHWKE"
     gene            835..1644
                     /locus_tag="VFY44_07545"
     CDS             835..1644
                     /locus_tag="VFY44_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910332.1"
                     /translation="MELLTVAQEQAVPGWVIAVAFAVPGVIMLLVLRWALRGRARQGR
                     HRRADERRRGGDALARATAAEIPDGGEYKNEALLRALSIRPEDHGPDDQGNPHDEGWA
                     ATMLGLRSKVSSATDLLEPHLYWGPRAAGQAFVRIGPDEKIAGGSELYSERHLRSITV
                     IRADAPEFELEGSAGRLRAKGAAPAALTRALEQIGASPDLWDRVLVVGGPKGIVATRP
                     SGSAEFGGWVYDLWLLERLAAVMELPPLPNARVGPAWKVPYGLGRSDKPQR"
     gene            1693..2316
                     /locus_tag="VFY44_07550"
     CDS             1693..2316
                     /locus_tag="VFY44_07550"
                     /inference="COORDINATES: protein motif:HMM:NF014646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HEX5910333.1"
                     /translation="MILTDERDRLSRHMLDALMAGNAQRASGVAGEAFDAGLTLTDLF
                     DDVLAPALCAVGERWQAGLVRHTQERFATTAAEALLDRIAPRDTGAGGPGWVVASLDG
                     EQHRVGVRMAVATLTDAGLRARPLFGTSAAEVVAAAEGAPGVCLSCSGPWSVVAARSA
                     AEQIAAAGVPVLLGGGGWGGWQQPPLIRLVGSMAELAAMAGDRTASL"
     gene            complement(2313..3851)
                     /locus_tag="VFY44_07555"
     CDS             complement(2313..3851)
                     /locus_tag="VFY44_07555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5910334.1"
                     /translation="MERSGGRNKNLVLVAMIFAVAMMFIDQTIVSIAVPELQKDLSLS
                     STGVQWIVNGYLLSLSALFAFGGRLADIAGHRRMVLVGVIGFATASALCGATPTGGAG
                     EAWIIFFRVVQGAFAAVLFPAALAIVVQAFPLDERGKAMATFFGITGGLTAIGPLAGG
                     YLTEYTWRAIFWVNVPVALIAVLLILRSKPDDARSPAPLDYRGTLLISGGMGLAVLGL
                     QQSATWGWSDPVTWLCIAAGLVLLVAFVFVERTTEHPLMDVRIFQSRPFTVDNVLLFL
                     LSMAFVPLFFFASVYAQIALGDDASQAGLYLLVFFGGFAAAAQVGGRILDKRGARPAA
                     VPGCLVAAIGFGLWAHKMTDLDFNAQWHLVALTGIGTGLVLGPVSTDALNRAKRGSYG
                     EVTGITQTFRNFGASVGLAVLGTILTLQNKAKVEETLSAAGLPKARADQIADAISHAG
                     GGSSSSFGGRTNPEEARLFGQIQMDFAQACQVVFYVMAGVMLAAFLVALVGMASGKVE
                     ETVA"
     gene            complement(3912..4103)
                     /locus_tag="VFY44_07560"
     CDS             complement(3912..4103)
                     /locus_tag="VFY44_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910335.1"
                     /translation="MSPVTASGRSECQICGCHTSVGACGNCGSSALLALPTPDVVVTM
                     EDALAADLCGRVEAGAPEG"
     gene            complement(4263..>4518)
                     /locus_tag="VFY44_07565"
     CDS             complement(4263..>4518)
                     /locus_tag="VFY44_07565"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX5910336.1"
                     /translation="DGFDAAYARARETAGDDGVDIAGGASTVRQALAAGVVDELTLDI
                     APVLLGAGERLFDDVEGLRLEPVEVLHSPAATHIRYAVGS"
BASE COUNT          655 a         1597 c         1650 g          616 t
ORIGIN      
        1 ctccttccag tgctcctcga gcgcctcgag caggtcgggc ggcccggaga tgtaggtctc
       61 gcgctccttc cagtccgggc acagctcgtc caggtccgcg ggcttgaacc gcccgacgtc
      121 ggtggcggtg tgctgctcgt ggagcacgaa cttctcgtgc ttgtcggcca gcgcgcgcat
      181 ctcgtcgccg aagatgacct cctcgggcgt gcccgcggag tggatgtgca tcgtgtccgg
      241 catgtcgccc tcgtggtcca gcgagcgcag catgctcatg atcggcgtga tcccgctgcc
      301 ggcgctgatg aacaggatct tctccggcac cgggtcgggc agcaggaagt caccctccac
      361 gccgcccagg ccgacgattg cgccgtgcgg tcccttgcgg accaggtacg gcgacaccac
      421 gccctcgtcc acgttcttga cggtgatcga gatgcagccg tcctcgcgct tgggatcgga
      481 cgtcagcgag taggcgcgcc agtgccggcg gccgttcagg tcgaccccga tgcgcaggta
      541 ctggccgggc tcgtggccct cccactcgta gcccggctgg atcacgacgg tggccgcgtc
      601 gggggtctcc tccttgacgt ccaccacctt gccgcgcagc tcgcgggtgg accacagcgg
      661 gttgatcagc tccaggtagt cgtccggcag cagcggcgac gtgaacgcgc tgatgaagcg
      721 cagcacacga cgctggacgc gcgggacttt cggcggggca cctctctcgg ccatggcggg
      781 accatagcga ccttgacacc gatcggtgta agtgtcctgg cgtagggtct cgccgtggag
      841 ctgctgacgg tggcccagga gcaggccgtg cccggctggg tgatcgccgt tgcgttcgcc
      901 gtgccgggcg tgatcatgct gctcgtgctg cgctgggccc tgcgcggacg agcgcgccag
      961 ggacgccaca gacgggccga cgagcggcgc cgtggcggcg atgcgctggc gcgcgccacg
     1021 gccgcggaga tccccgacgg cggcgagtac aagaacgagg cgctgctgcg cgcgctctcg
     1081 atcaggcccg aggaccacgg ccccgacgac cagggcaacc cccacgacga gggctgggcg
     1141 gccacgatgc tggggctacg gtcgaaggtc agcagcgcca ccgacctgct cgagccccac
     1201 ctgtactggg gcccgcgggc cgcgggccag gccttcgtgc ggatagggcc cgacgagaag
     1261 atcgccggcg gcagcgagct gtactccgag cgccacctgc ggtcgatcac ggtgatccgc
     1321 gcggacgcgc ccgagttcga gctggaggga tcggcgggcc gcctccgggc gaagggcgcc
     1381 gcgccggccg ccctgacccg ggcgctcgag cagatcggcg ccagcccgga cctgtgggac
     1441 cgggtgctgg tggtcggggg gccgaagggc atcgtggcca cccgcccgtc cggcagcgcc
     1501 gagttcgggg gctgggtcta cgacctgtgg ctgctcgagc ggctggctgc ggtgatggag
     1561 ctcccgccgc tgccgaacgc gcgcgtggga ccggcctgga aggtgccgta cggcctgggc
     1621 cggtcggaca agccacagcg ttaacgccat cgacgcgggc gaaatccggc gatctgcccg
     1681 atagtggtcg gcgtgatcct gaccgacgag cgcgaccggc tcagccggca catgctggac
     1741 gcgctcatgg ccggcaacgc gcagcgcgcc tccggcgtgg ccggcgaggc gttcgacgcg
     1801 gggctgacgc tgaccgacct gttcgacgac gtgctggcgc ccgcgctgtg cgcggtcggc
     1861 gagcggtggc aggccggcct ggtgcgccac acccaggaac ggttcgccac gacggccgcc
     1921 gaggcgctgc tggaccggat cgcgccgcgc gataccggcg ccggaggccc cgggtgggtc
     1981 gtggccagcc tggacggcga gcagcatcgc gtgggcgtgc gcatggccgt ggccacgctg
     2041 accgacgccg gcctgcgggc gcgaccgctg ttcggcacct ccgcggccga ggtggtggcc
     2101 gcggccgagg gcgcgcccgg cgtgtgcctg tcgtgctcgg gcccctggag cgtggtggcc
     2161 gcgcgcagcg cggcggagca gatcgcggcc gccggcgtcc ccgtgctgct gggtggcggc
     2221 ggttggggcg gctggcagca gccgccgctg atcaggctcg tcgggtcgat ggccgagctg
     2281 gcggcgatgg ccggcgaccg caccgcttcg ctctaggcga ccgtctcctc gaccttgccg
     2341 gacgccatgc ccaccagggc gaccaggaac gcggccagca tcacgccggc catgacgtag
     2401 aagaccacct ggcaggcctg ggcgaagtcc atctggatct ggccgaacag gcgcgcctcc
     2461 tccgggttcg tcctcccgcc gaagctggac gacgagccgc cgccggcgtg gctgatggcg
     2521 tcggcgatct ggtcggcgcg ggccttcggc aggcctgccg cggacagcgt ctcctcgacc
     2581 ttcgccttgt tctgcagggt caggatcgtg cccagcacgg ccaggcccac gctggccccg
     2641 aagttgcgga aggtctgggt gatgccggtg acctcgccgt agctgccgcg cttggcgcgg
     2701 ttgagcgcgt cggtgctcac ggggcccagg accaggccgg tgccgatgcc ggtcagcgcg
     2761 accaggtgcc actgggcgtt gaagtccagg tcggtcatct tgtgcgccca caggccgaac
     2821 ccgatcgccg cgaccaggca gcccggcacg gcggcggggc gcgcgccgcg cttgtccagg
     2881 atccggccgc cgacctgggc cgcggcggcg aagcccccga agaacaccag gaggtacagg
     2941 ccggcttggg aggcgtcgtc gcccagggcg atctgcgcat agacgcttgc gaagaagaac
     3001 agcggcacga acgccatcga cagcaggaac agcaggacgt tgtcaacggt gaacggacgc
     3061 gactggaaga tgcgcacgtc catcagcggg tgctcggtgg tgcgctcgac gaacacgaac
     3121 gccaccagca gcaccagccc ggccgcgatg cacagccacg tcaccggatc gctccagccc
     3181 cacgtcgccg actgctgcag gccgagcacg gccaggccca tgccccccga gatcagcagc
     3241 gtgccgcggt agtccagcgg cgccgggctg cgggcgtcgt cgggcttgga cctgaggatc
     3301 aacagcacgg cgatcagggc caccggcacg ttgacccaga agatcgcccg ccacgtgtac
     3361 tcggtcaggt acccgccggc cagcgggccg atggcggtga ggccgccggt gatgccgaag
     3421 aacgtggcca tggccttgcc gcgctcgtcg agggggaagg cctggacgac gatcgccagc
     3481 gccgccggga acaacaccgc ggcgaacgcc ccctgcacga cccggaagaa gatgatccag
     3541 gcctcgcccg cgccgcccgt cggcgtggcc ccgcacagcg ccgacgcggt ggcgaacccg
     3601 atcaccccga ccaggaccat cctgcggtgc ccggcgatgt cggccagccg gcccccgaac
     3661 gcgaacaacg ccgacagcga caacaggtag ccgttgacga tccactgcac cccggtcgag
     3721 gacagcgaga ggtccttctg gagctcgggg accgcgatcg agacgatcgt ctggtcgatg
     3781 aacatcatcg cgacggcgaa gatcatcgcg acgaggacga ggttcttgtt gcgtccgcct
     3841 gaacgctcca tgggacgacg ctataccggt taaccccgtg gccgcaaggg cgacggccac
     3901 gcgagcgagc gtcagccctc gggcgcgcct gcttcgacgc gcccgcacag gtccgcggcg
     3961 agcgcgtcct ccatggtcac gaccacgtca ggcgtcggca gggccagcag cgcgctgcta
     4021 ccgcagttgc cgcaggcgcc gacggacgtg tggcacccgc agatctggca ttcgctccgg
     4081 cccgaggccg tgactggact catggtgctg ctttcctcgt cggcgcttcg tcgaagtgtg
     4141 gctgcgagag ggctagaagc cgacgagcga agagctctcg ggcagatacg ccacttcgag
     4201 tatcggcgcg ttccccgcgt ttcgcctcat ccgatcggat gagtgcgggg atccggcgag
     4261 cctcagctcc cgacggcgta cctgatgtgc gtcgccgccg gcgagtgcag cacctcgacc
     4321 ggctcgagcc ggaggccctc cacgtcgtcg aacagccgct cgcccgcccc gagcagcacc
     4381 ggcgcgatgt cgagcgtcag ctcgtcgacc acgccggcgg ccagcgcctg gcgcaccgtg
     4441 gaggcgccgc cggcgatgtc cacgccgtcg tcgcccgccg tctcacgcgc ccgtgcgtag
     4501 gcggcgtcga agccgtcg
//
LOCUS       DASUPW010000337         3562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1050680, whole genome shotgun sequence.
ACCESSION   DASUPW010000337 DASUPW010000000
VERSION     DASUPW010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050680"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="VFY44_07570"
     CDS             complement(<1..1160)
                     /locus_tag="VFY44_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX5910337.1"
                     /translation="MRTSIATVSLSGSLEDKLAAAAGAGFDGVEIFEPDLVASPLSPA
                     HVREHAAALGLSIDLYQPFRDFEGVTPERLEANLRRARRKFELMAELGADTMLVCSNV
                     APDAIDDPGLSAAQLYRLAEEAQQHGVRIAFEALAWGRHISDYRAAWAVVERAGHPAL
                     GTCLDSFHILSRGSDPAEIERIPGQKVFFLQLADAPVLGMDVLQWSRHHRCFPGQGGF
                     DLAAFMGHVQATGYDGPWSLEVFNDHFRQAEPERIAVDARRSLLALGEQVGAATVPPA
                     PRAERFAFAELAVGPGSGDRAAEVLEAMGFAHVGPHRSKPVHQWRQGDARVLLNHGDG
                     NAGDDARVSALGVESADPDGAAARAEDLGAALLPRRRGPGEADLAAIAAPDDT"
     gene            complement(1157..2035)
                     /locus_tag="VFY44_07575"
     CDS             complement(1157..2035)
                     /locus_tag="VFY44_07575"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF009201.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX5910338.1"
                     /translation="MGSENGDIHQGERVEVALVGTGIERSLSPALHEREGALLGFDYE
                     YRLIDLPAVAPIGEVVERCRWDGLRGLNVTHPFKQLVLDALDRLSPEAAALGAVNTVV
                     FGPHGAEGHNTDTTGFRDAFAAGLPGAAIDRVVVVGAGGAGAAVAYALLGLDAGQVRV
                     VDRDRERAAELVAKLDEHFGAGRAVAADSVALDDADGVVHATPTGMGGGSRSAIDPAW
                     LHPRLWVAEVVYVPLDTQLLRDAREAGCTTLDGGGMVAAQAAGSLELFTGAAPDRERM
                     TAHMAELVAGQREAAR"
     gene            2108..2743
                     /locus_tag="VFY44_07580"
     CDS             2108..2743
                     /locus_tag="VFY44_07580"
                     /inference="COORDINATES: protein motif:HMM:NF037007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5910339.1"
                     /translation="MASPATGERQRDAERTRAEILEVATREFAEQGFAGARVDEIADR
                     TRTTKRMLYYYFGGKEQLYQEVLQQAYATIRAAEQTIEVDHLEPTAAIRRLAELTFDH
                     HESHPDFIRLVSIENIHHAEHIAGLEDFAGQNSPVIELIDRILKRGLASGEFTRKVDA
                     VDLHMLISSFCVFRVANRHTFGAIFDRDLTSPRLRKRYREMLGDVVVGYLR"
     gene            complement(2740..3147)
                     /locus_tag="VFY44_07585"
     CDS             complement(2740..3147)
                     /locus_tag="VFY44_07585"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX5910340.1"
                     /translation="MEADVHHLLTATRTWAVVGCSPDPRRDSNRIAALLQSRGYRVIP
                     VNPAVDGELLGERCYPRLGDIPESENVEVVDIFRRADQAGAHVDEAIAAGVAGVWMQL
                     GVVDAAAAERARAAGLHVVMDRCPAIELPRLAA"
     gene            3171..>3562
                     /locus_tag="VFY44_07590"
     CDS             3171..>3562
                     /locus_tag="VFY44_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910341.1"
                     /translation="MGPTVAIAFHGLTAAEDLDAGRPDAPTVSPALPTILRALADRDI
                     DATFYVGDELREREPLAVTMIRNGRHAVGSPADEPDPAVRDTPYLLDQGQEGGPAGAN
                     ALHEALQLAVAGALRGRSDAVLLFTPGLL"
BASE COUNT          486 a         1371 c         1266 g          439 t
ORIGIN      
        1 gtgtcgtcgg gcgcggcgat ggcggccagg tcggcctcgc ccgggccgcg gcggcgcggc
       61 agcagcgccg caccaaggtc ctcggcgcgc gcggccgcgc cgtcggggtc ggcgctctcc
      121 acacccaggg ccgacacccg cgcgtcatcg cccgcgttgc cgtcgccgtg gttgagcagt
      181 acccgcgcgt cgccctggcg ccactggtgc accggcttgg accggtgcgg gcccacgtgg
      241 gcgaagccca tggcctccag cacctcggcg gcccggtcgc ccgagccggg ccccacggcc
      301 agctcggcga aggcgaaccg ctccgcccgc ggggcgggcg gcaccgtggc ggcgcccacc
      361 tgctcgccca gggccagcag cgagcgccgc gcgtcgacgg cgatgcgctc gggctcggcc
      421 tggcggaagt ggtcgttgaa gacctcgagc gaccacggcc cgtcgtagcc cgtggcctgc
      481 acgtggccca tgaaggcggc caggtcgaac ccgccctggc ccgggaagca gcggtggtgt
      541 cggctccact ggagcacgtc catgcccagc acgggcgcgt cggccagctg gaggaagaag
      601 accttctgcc cggggatgcg ctcgatctcg gccggatcgc tgccgcggga gaggatgtgg
      661 aacgagtcca ggcaggtgcc cagcgcggga tggccggcgc gctccaccac cgcccacgcg
      721 gcgcggtagt cgctgatgtg ccgcccccag gccagcgcct cgaaggcgat ccgcacgccg
      781 tgctgctgcg cttcctcggc cagccggtac agctgcgcgg ccgacagccc ggggtcgtcg
      841 atggcgtccg gagccacgtt ggagcagacc agcatcgtgt ccgcgcccag ctcggccatg
      901 agctcgaact tgcgccgcgc gcggcgcagg ttggcctcca ggcgctcggg cgtcacgccc
      961 tcgaagtcgc ggaagggctg gtagaggtcg atcgacaggc cgagggccgc ggcgtgctca
     1021 cgcacgtgcg cgggcgacag cggcgacgcc accaggtcgg gctcgaagat ctcgacaccg
     1081 tcgaagcccg cgcccgcggc ggccgcgagc ttgtcctcca gcgacccgct gagcgacacg
     1141 gtggcgatcg aggtcctcat cgcgccgcct cccgctggcc cgccaccagt tcggccatgt
     1201 gcgcggtcat gcgctcgcgg tcgggcgccg cgccggtgaa gagctccagc gaccccgccg
     1261 cctgggcggc caccatgccg cccccgtcca gcgtggtgca gccggcctcg cgggcgtccc
     1321 gcagcagctg ggtgtccagg ggcacgtaga ccacctcggc cacccacagg cgcgggtgca
     1381 gccacgcggg atcgatggcg ctgcggctgc cgccgcccat gccagtgggg gtggcgtgga
     1441 ccaccccgtc ggcgtcgtcc agcgcgaccg agtccgccgc caccgcgcgc ccggcaccga
     1501 agtgctcgtc gagcttggcc accagctccg ccgcccgctc ccggtcacgg tcgaccaccc
     1561 tgacctggcc ggcgtccagg cccagcagcg cgtaggcgac cgccgccccg gcgccgccgg
     1621 cgccgacgac caccacgcga tcgatggccg cgccgggcaa cccggcggcg aacgcatcac
     1681 ggaagccggt ggtgtcggtg ttgtgccctt cggcgccgtg ggggccgaag accacggtgt
     1741 tgaccgcgcc cagcgcggcc gcctcggggg acagccggtc cagggcatcc agcaccagct
     1801 gcttgaacgg gtgggtgacg ttcaggccgc ggaggccgtc ccagcggcag cgctcgacca
     1861 cctccccgat cggcgcgacc gcgggcaggt cgatcagccg gtactcgtag tcgaagccca
     1921 gcagcgcgcc ctcgcgctcg tgcagggccg ggctcaacga gcgctcgatg cccgttccca
     1981 cgagcgcgac ctcgacgcgc tcgccctggt ggatgtcccc gttctcgctc cccatcggat
     2041 cgcctaatgt acgaaccagt gagttagttc ccgccgggga ggccgcccaa acccgtaggc
     2101 ttgcccggtg gcctcaccgg cgaccggaga acggcagcgc gacgccgagc gcacccgcgc
     2161 cgagatcctg gaggtggcca cccgggagtt cgccgagcag ggcttcgcgg gcgcgcgcgt
     2221 ggacgagatc gccgaccgca cccgcaccac caagcggatg ctctactact acttcggcgg
     2281 caaggagcag ctctaccagg aggtgctcca gcaggcctac gccaccatcc gcgcggccga
     2341 gcagaccatc gaggtcgacc acctggagcc caccgccgcc atccgccggc tggccgagct
     2401 gaccttcgac caccacgagt cgcaccccga cttcatccgc ctggtcagca tcgagaacat
     2461 ccaccacgcc gagcacatcg cgggcctgga ggacttcgcc ggccagaaca gcccggtgat
     2521 cgagttgatc gaccggatcc tcaagcgcgg gctggccagc ggcgagttca cgcgcaaggt
     2581 cgacgccgtg gacctgcaca tgctgatcag ctcgttctgc gtcttccggg tggccaaccg
     2641 ccacaccttc ggggcgatct tcgaccgtga cctgacctcg ccgcggctgc gcaagcgcta
     2701 ccgcgagatg ctgggcgacg tggtcgtggg ctacctgcgc taggcggcca ggcgcggcag
     2761 ctcgatggcc gggcagcggt ccatgaccac gtgcagcccc gcggcgcgtg cgcgctcggc
     2821 ggcggccgcg tcgaccacgc ccagctgcat ccacaccccg gccacgccgg ccgcgatcgc
     2881 ctcgtccacg tgggcgccgg cctgatcggc gcggcggaag atgtccacca cctcgacgtt
     2941 ctcggactcc gggatgtcgc cgagcctcgg atagcagcgc tcgcccagca gctcgccgtc
     3001 gacggccggg ttgaccggga tcacgcggta gccgcggctc tggagcaggg cggcgatccg
     3061 gttcgagtcc cggcgcggat cgggtgagca gccgaccacg gcccaggtgc gggtggcggt
     3121 cagcaggtgg tggacgtcgg cttccatgca tcccagccta ggctcaggtc atgggcccga
     3181 ccgtcgccat cgccttccac gggctgaccg ccgccgagga cctggacgcg ggcaggcccg
     3241 acgcgcccac cgtcagcccg gccctgccca ccatcctgcg cgccctggca gaccgcgaca
     3301 tcgacgccac cttctacgtc ggggacgagc tgcgcgagcg cgagccgctg gccgtgacca
     3361 tgatccgcaa cggccggcac gccgtcggct ccccggccga tgagcccgac ccggcggtgc
     3421 gcgacacgcc gtacctgctg gaccagggcc aggagggtgg gcccgccggc gccaacgcgc
     3481 tgcacgaggc gctccagctg gccgtggcgg gcgcgctccg cggccggtcc gacgccgtgc
     3541 tgctgttcac ccccgggctg ct
//
LOCUS       DASUPW010000338         2282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1466560, whole genome shotgun sequence.
ACCESSION   DASUPW010000338 DASUPW010000000
VERSION     DASUPW010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466560"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..795
                     /locus_tag="VFY44_07595"
     CDS             61..795
                     /locus_tag="VFY44_07595"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5910342.1"
                     /translation="MAEPLTLYVIHGSHPCASVEAGLRTKGLAYDTVYLQFGLSQFTQ
                     TARFGKRTVPGLTVGIQKASGSRLILRALDGLSAQAPLLPADPELRGAVDEADLWGDE
                     VLQHQVRWIAVDAIRREPSVALAYGSKSAPPLPDAAIPGATRALFTTEMRLLGQTAER
                     VRDEYVPALAEHLDHVDGLIADGVLGGEQVNVADCQVAGSIRLLQTFEDLREGIDARP
                     CGALARRLCPQYDGSMPRGVIDAPVL"
     gene            813..1841
                     /locus_tag="VFY44_07600"
     CDS             813..1841
                     /locus_tag="VFY44_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX5910343.1"
                     /translation="MASIFSRAVGGGRDIAVDLGTANTLVYVAGQGIVASEPSVVAID
                     ENTGEVHAVGQEAQRMIGRTPASISAVRPLRHGVIADFEITEEMLRYFIRRVHQSRFA
                     HPRVVMCAPSGITDVERNAVREACLAAGARKVHLIEEPIAAAIGAGLDIGEPTGRLVV
                     DVGGGTSEVAVISLGGMVVSRSLRVGGYELDEAIAAYTRSEHNLAIGERSAEEIKLEL
                     GTARPMPTEEHTELRGRDLVSGLPKSIMLSSGQIREAIAEPVNAIVECVRETLEETPP
                     ELASDITESGILLAGGGSLLRGFAERLTEETHIDAYLAESPLTCVALGSGYSLEEFEA
                     LERTSARG"
     gene            complement(1850..>2282)
                     /locus_tag="VFY44_07605"
     CDS             complement(1850..>2282)
                     /locus_tag="VFY44_07605"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HEX5910344.1"
                     /translation="AALPYALGSMAAEPDTHLDDDRPELLRKLEERKARHAERGMFAR
                     VGVVFLGIVLVLLGILMSGPGIPGPGIVVILLGLSFLALEFDWAERLLGKAIVWADNA
                     KDRAEAASPRQKAVTAGLAVLALAAFVVAAVLWDIPLLPVV"
BASE COUNT          329 a          816 c          843 g          294 t
ORIGIN      
        1 gcctgatccc gaccggaacg cgatcgcctt cgcggaggtg cggcggggta ggctcccgcc
       61 gtggccgaac ccctgacgct gtacgtgatc cacggctcgc acccgtgcgc gagcgtggag
      121 gcggggctgc ggaccaaggg gctcgcctac gacaccgtct acctgcagtt cggcctgtcg
      181 cagttcaccc agaccgcgcg cttcggcaag cgcaccgtcc ccgggctgac cgtggggatc
      241 cagaaggcat cgggctcgcg tctgatcctg cgggcgctgg acggcctgtc ggcccaggcg
      301 ccgctgctgc ccgccgaccc ggagctgcgc ggcgctgtcg acgaggccga cctgtggggc
      361 gacgaggtgc tccagcacca ggtgcgctgg atcgccgtgg acgccatccg gcgcgagccc
      421 tcggtggcgc tggcgtacgg ctcgaagtcg gcgccgccgt tgccggacgc ggccatcccg
      481 ggtgccacca gggccctgtt caccaccgag atgcggctgc tgggccagac ggccgagcgg
      541 gtgcgcgacg agtacgtgcc cgcgctggcg gaacacctgg accacgtgga cggcctgatc
      601 gccgacggcg tcctgggtgg cgagcaggtc aacgtggccg actgccaggt ggcgggcagc
      661 atccgcctgc tccagacctt cgaggacctg cgcgagggca tcgacgcgcg cccctgcggc
      721 gcgctggccc ggaggctgtg cccgcagtac gacggcagca tgccacgggg cgtgatcgac
      781 gccccggtcc tgtagctgcg agccttcggc ggatggcatc gatcttcagc cgggccgtgg
      841 ggggcggccg ggacatcgcc gtggacctcg gtaccgccaa cacgcttgtg tacgtggcgg
      901 ggcagggaat cgtggcctcc gagccctcgg tggtggccat cgacgagaac acgggcgagg
      961 tgcacgcggt gggccaggag gcccagcgca tgatcggccg gaccccggcg tcgatctccg
     1021 ccgtgcggcc gctgcgccac ggcgtgatcg ccgacttcga gatcaccgag gagatgctgc
     1081 gctacttcat ccggcgcgtg caccagagcc gcttcgcgca tccgcgggtg gtcatgtgcg
     1141 ccccctcggg gatcaccgac gtggagcgca acgcggtccg cgaggcgtgc ctggccgccg
     1201 gcgcccggaa ggtccacctg atcgaggagc cgatcgccgc cgccatcggc gccgggctgg
     1261 acatcggcga gcccaccggc cgcctggtgg tggacgtggg cggcggcacc agcgaggtgg
     1321 cggtgatctc gctggggggc atggtggtct cacgctcgct ccgggtgggg ggctacgagc
     1381 tggacgaggc catcgcggcg tacacacgca gcgagcacaa cctggccatc ggcgagcgct
     1441 cggccgagga gatcaagctc gagctgggca ccgcccgccc gatgcccacc gaggagcaca
     1501 ccgagctccg cggccgcgac ctcgtctccg gtctgcccaa gagcatcatg ctcagcagcg
     1561 gccagatccg cgaggccatc gccgagccgg tcaacgccat cgtggagtgc gtgcgcgaga
     1621 cgctcgagga gacgccgccc gagctggcct ccgacatcac cgagagcggc atcctgctgg
     1681 ccggaggcgg ctcgctgctg cgcggattcg ccgagcggct gaccgaggag acgcacatcg
     1741 acgcctacct ggccgaatcg ccgctgacgt gcgtggcgct cgggtcgggc tactcgctgg
     1801 aggagttcga ggcgctcgag cgcacgtccg cgcgcggcta gccgcgcgat cagacgacgg
     1861 gcaagagcgg gatgtcccag agcacggcgg ccaccacgaa cgccgccagc gccagcacgg
     1921 cgagcccggc cgtgacggcc ttctgacgcg ggctggccgc ctcggcgcgg tccttggcgt
     1981 tgtcggccca gacgatcgcc ttgcccagca gccgctcggc ccagtcgaac tccagcgcca
     2041 ggaagctcag gcccagcagg atcaccacga tgcccgggcc cgggatcccg gggcccgaca
     2101 tgaggatccc gagcaggacc agcacgatgc ccaggaacac gacgcccacg cgggcgaaca
     2161 tcccgcgctc ggcgtgacgg gccttgcgct cctcgagctt gcgcagcagc tccggccggt
     2221 cgtcgtccag atgggtgtcg ggctccgctg ccatcgagcc cagagcgtag ggcagcgcgg
     2281 cc
//
LOCUS       DASUPW010000339         2917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_912216, whole genome shotgun sequence.
ACCESSION   DASUPW010000339 DASUPW010000000
VERSION     DASUPW010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912216"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VFY44_07610"
     CDS             <1..346
                     /locus_tag="VFY44_07610"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX5910345.1"
                     /translation="GHPVVVNKWGSWCAPCRAEFPALQRQAVKQGKRVAFLGVDGQDN
                     DDNARKFLEKYPVAYPSYVDGDLSIARSFGAVQGFPSTVFYDRKGEIAYVKQGSYPSE
                     AALARDIRRYAR"
     gene            360..779
                     /locus_tag="VFY44_07615"
     CDS             360..779
                     /locus_tag="VFY44_07615"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5910346.1"
                     /translation="MRAPSSDAEMEAAMELRHEVFCVEQGVTVEADRDGMDPQAEHLV
                     AEQDGRVVGTCRLLIDGHVARLGRMVVSRSVRGRGVGGLILAEADRVAAAHGATRILL
                     HAQVAARGVYDRAGYLPTSDVFVEEGIEHVSMEKHLA"
     gene            772..1551
                     /locus_tag="VFY44_07620"
     CDS             772..1551
                     /locus_tag="VFY44_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910347.1"
                     /translation="MPELRVDPLSGLRTVIGDPGTGWDDAGEQPRPDPLGGGRGEPDL
                     FAASPGAGALELVDHPRALSEQDPDELAATLEGWRERMRAHAGAAYVHLWADPRRTHV
                     HALPFVPARVARERERFTAYRERTQGRNLQADLLQAEVRNRERVVSVGPAVVALCPFA
                     SMAPLHVQLLPRAERPRFEDDGPTGADTLHDVLRRLAALSAAEPRIWVRTAPPGATAF
                     CWRIDIVPAAGPDDGLAAGAGVSLTALAPEDAAARLRDALR"
     gene            1563..2174
                     /locus_tag="VFY44_07625"
     CDS             1563..2174
                     /locus_tag="VFY44_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910348.1"
                     /translation="MPPSARPIPAYAAQPPQEPRPYGRWGQALTERFHDAARALESEE
                     DLGEPGEVVWFPDRTWGGRTYVPATASTSTGFELFGYVAYTREHEGAQAEDFTTSVDW
                     TDETAEANPDWTLDLSDQEIAEWRGPDGQHGVITLVWGVSLVANGRVATAELGPETTD
                     QCVLVDDRFTLVSLDDYVGDLLEIRLYAQKGAELARESLYEDD"
     gene            2188..2261
                     /locus_tag="VFY44_07630"
     tRNA            2188..2261
                     /locus_tag="VFY44_07630"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2222..2224,aa:Arg,seq:cct)
     gene            complement(2290..2682)
                     /locus_tag="VFY44_07635"
     CDS             complement(2290..2682)
                     /locus_tag="VFY44_07635"
                     /inference="COORDINATES: protein motif:HMM:NF021568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HEX5910349.1"
                     /translation="MTESRDGAVDVETLSEEGDVRTTISDGIVALLKEYYGRGPDRTK
                     TYVSGDLVVCLLRGGFTPVEQTLLEGGHGDDVIRQRMAFQQVMRRRFEEVVEGATGRK
                     VIGFMSGNHQEPDMLCEVFVLAQNSMLD"
BASE COUNT          402 a         1063 c         1052 g          400 t
ORIGIN      
        1 gggccacccc gtcgtggtga acaagtgggg gtcgtggtgc gcgccctgcc gcgccgagtt
       61 ccccgcgctc cagcgccagg cggtgaagca gggcaagcgg gtggcgttcc tgggcgtcga
      121 cggccaggac aacgacgaca acgcgcgcaa gttcctggag aagtacccgg tcgcctaccc
      181 ctcgtacgtg gacggggacc tgtcgatcgc gcgctccttc ggcgcggtgc agggctttcc
      241 gtcgaccgtg ttctacgacc gcaaggggga gatcgcgtac gtcaagcagg gcagctaccc
      301 cagcgaggcc gccctggcac gggacatccg ccgttacgcc cgctgacccc ccgatcaccg
      361 tgcgcgcccc ctcgtccgac gccgagatgg aggcggccat ggagctgcgc cacgaggtct
      421 tctgcgtcga gcagggggtg accgtcgagg ccgaccgcga cggcatggac ccgcaggccg
      481 agcacctggt ggccgagcag gacgggcggg tcgtgggcac ctgccggctc ctgatcgacg
      541 ggcacgtggc ccggctgggg cggatggtgg tgagccggtc cgtgcgcggc cgtggcgtgg
      601 gcggcctcat ccttgccgag gcggacaggg tggccgcggc gcacggcgcg acgcgcatcc
      661 tcctgcatgc ccaggtggcg gcccggggcg tgtacgaccg cgcgggctac ctccccacgt
      721 cggacgtgtt cgtggaggag ggcatcgagc acgtgtccat ggagaagcac cttgcctgag
      781 ctgcgcgtgg acccgttgtc gggcctgcgg accgtgatcg gcgaccccgg gacgggctgg
      841 gacgacgccg gcgagcagcc acgccccgac ccgctgggcg gcggccgcgg cgagccggac
      901 ctgttcgccg cctcgccggg cgccggtgcg ctggagctgg tggaccatcc gcgtgccctg
      961 tccgaacagg acccggacga gctggccgcg acgctggaag gctggcgcga gcggatgcgc
     1021 gcgcacgccg gtgcggcgta cgtgcacctc tgggccgacc cgcgccggac gcacgtgcac
     1081 gcgctgccct tcgtccccgc acgggtggcc cgcgagcgcg agcggttcac cgcgtaccgc
     1141 gagcgcaccc agggacgcaa cctccaggcc gacctcctcc aggccgaggt gcgcaaccgc
     1201 gagcgggttg tgtcagtggg ccccgcggtg gtcgcgctgt gcccgttcgc ctccatggct
     1261 ccactgcacg tgcagctgct gccccgcgcc gagcgccccc ggttcgagga cgacgggccg
     1321 accggcgccg acaccctgca cgacgtgctg cggcggctgg ccgccctgag tgcggccgag
     1381 ccgaggatct gggtccggac cgcgccgccg ggcgccaccg cgttctgctg gcggatcgac
     1441 atcgtcccgg ccgcggggcc ggacgacggc ctggctgcgg gcgcgggcgt gagcctgacg
     1501 gcgctggcgc cggaggacgc ggcggcgagg ctgcgcgacg ccctgcgcta gcttgcgcgc
     1561 cgatgcctcc ttcagcgcgc cccatccccg cctacgccgc ccagccgcca caggagcccc
     1621 gcccgtacgg gcgctggggc caggccctga ccgagcgctt ccacgacgcc gcccgggccc
     1681 tggagtccga ggaggacctg ggcgagccgg gcgaggtcgt ctggttcccc gaccgcacct
     1741 ggggcgggcg cacctacgtc ccggccaccg cgtccacctc gaccgggttc gagctgttcg
     1801 gctacgtggc gtacacccgc gagcacgagg gcgcgcaggc cgaggacttc accacctccg
     1861 tggactggac cgacgagacc gccgaggcca accccgactg gaccctggac ctgtccgacc
     1921 aggagatcgc ggagtggcgt ggccccgacg ggcagcacgg cgtgatcacg ctcgtctggg
     1981 gcgtgtccct ggtggccaac gggcgggtgg cgaccgccga gctggggccc gagaccaccg
     2041 accagtgcgt gctggtggac gaccgcttca cgctggtctc gctggacgac tacgtgggcg
     2101 acctgctgga gatcaggctc tacgcgcaga agggcgcgga gctcgcgcgc gagtcgctgt
     2161 acgaagacga ttaacttccc cgcaccggcc ccgatagctc agctggatag agcgtccccc
     2221 tcctaagggg aaggccgctg gttcgaatcc agctcggggc atcggatggg tcgccggcgc
     2281 ggtccggcgt cagtcgagca tgctgttctg ggccagcacg aagacctcgc agagcatgtc
     2341 gggctcctgg tggttgcccg acatgaaccc gatcaccttg cggcccgtgg cgccttccac
     2401 gacctcctcg aatcgccgcc gcatcacctg ctggaaggcc atccgctggc ggatcacgtc
     2461 gtcgccgtgg ccgccttcga gcagcgtctg ctcgaccggg gtgaaccctc cccgcagcag
     2521 gcagacgacc agatcgccgc tgacgtaggt cttcgtccgg tcgggtccgc gcccgtagta
     2581 ctccttgagg agggccacga tcccgtccga gatcgtggtc cggacatcgc cttcctcgct
     2641 cagcgtctcg acgtctacgg cgccatccct cgattcggtc accgggcaag agtagccccg
     2701 atcaccctct agactgtgcc aacctcgcga ggttcttctg gccccctcca cctttcgcgg
     2761 acaacgcaga gggggtttgg aaggacgtcc gccatgtcgg acccagagag ccccgcggca
     2821 ttgaccgagg agacgctcga cgccgtcacg gcggcgctcg tggccctgca cgagcgctac
     2881 cacgggcgca agcccgtgac cgcgcgcacc cagatga
//
LOCUS       DASUPW010000340         2036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_912232, whole genome shotgun sequence.
ACCESSION   DASUPW010000340 DASUPW010000000
VERSION     DASUPW010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912232"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..618
                     /locus_tag="VFY44_07640"
     CDS             172..618
                     /locus_tag="VFY44_07640"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX5910350.1"
                     /translation="MSQDLREQREKLVREHMESENHHDFETTLGTFEHPRYEIVPTGD
                     VFDGAEAVQRYFDDTRTAFPDQRNELIALHHADDGVIVEFELKGTHQGPLRGIPATGK
                     EFTCRTAAFFLFEGEKLVCERVYFDAATIMAQLGLIQPAPTLQDGE"
     gene            615..1103
                     /locus_tag="VFY44_07645"
     CDS             615..1103
                     /locus_tag="VFY44_07645"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5910351.1"
                     /translation="MSAQAHKALIERFYTAFGQKDGHAMEECYASDVRFSDPVFPDLR
                     GPRAGAMWQMLTEAPGDLRIELLEHDAGEDTGSAHWRAHYVFSQTGRPVVNDVKASFT
                     FRDGLITEHHDDFNFHRWSRQALGLPGLLLGWTPMLKGAVRRKAADRLEEHTGVSAED
                     RY"
     gene            complement(1100..1546)
                     /locus_tag="VFY44_07650"
     CDS             complement(1100..1546)
                     /locus_tag="VFY44_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910352.1"
                     /translation="MEAGAATRVLVVANKTAATPALIEAVRERAAHGQATFTLLVPAS
                     AHGLHQLVDPEDQESTEAESVVALAVPLLEEAAGAPVEALVGAPEPLAAIEDAVNVHG
                     FDEIIISTLPTRVSRWMHLDLPRKVAGLGLPVTTVNAKGGQPTAAS"
     gene            complement(1601..>2036)
                     /locus_tag="VFY44_07655"
     CDS             complement(1601..>2036)
                     /locus_tag="VFY44_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910353.1"
                     /translation="DPPPYSPPPPGPVGYVSSPAGTPPPNKAAAPIALPSGSAPVVRA
                     DGAVFIRVGCPPGSPVTCGGFLLIEVPGTAGKSAAKLVLAGRSRYSVRPGRTGRVKVK
                     LNKATRKVLRRKRKLALRITIKPDGAKVVTRYKRTVRLPRRP"
BASE COUNT          289 a          750 c          719 g          278 t
ORIGIN      
        1 ccagttccgc accccgggcc tgcggctgtg gcagtccgcg gccacgagcc cctccgggat
       61 gaccatgttc ggccaggatg agctgacggg cgcgctgaag gccaacgggt tcgaggacgt
      121 gcgccggctg ctcagcggga ccacccagtt cgtgggcggg gtgctggccg gatgagccag
      181 gacctgcgcg agcagcgcga gaagctcgtc cgcgagcaca tggagtccga gaaccaccac
      241 gacttcgaga ccaccctcgg caccttcgag cacccccgct acgagatcgt gcccaccggc
      301 gacgtcttcg acggcgccga ggccgtccag cggtacttcg acgacacgcg caccgcgttc
      361 cccgaccagc gcaacgagct gatcgccctg caccacgccg acgacggcgt gatcgtggag
      421 ttcgagctca agggcaccca ccagggcccg ctgcgcggga taccggccac cggcaaggag
      481 ttcacctgcc ggaccgccgc gttcttcctg ttcgagggcg agaagctggt ctgcgagcgg
      541 gtctacttcg acgcggccac gatcatggcc cagctcggcc tgatccagcc cgccccaacg
      601 ctgcaggacg gcgagtgagc gcgcaggccc acaaggcgct gatcgagcgc ttctacaccg
      661 cgttcggcca gaaggacggc cacgcgatgg aggagtgcta cgcgtccgac gtgcgcttct
      721 ccgaccccgt gttcccggac ctgcgcgggc cccgcgcggg cgcgatgtgg cagatgctga
      781 ccgaggcgcc gggcgacctg cggatcgagt tgctggagca cgacgccggc gaggacaccg
      841 ggagcgccca ctggcgcgcg cactacgtgt tcagccagac cggccgcccg gtggtcaacg
      901 acgtcaaggc ctccttcacg ttccgggacg gcctgatcac cgagcaccac gacgacttca
      961 acttccaccg ctggtcacgc caggccctgg gcctgccggg gctgctgctg ggctggacgc
     1021 cgatgctcaa gggcgccgtg cggcgcaagg ccgcggaccg gctcgaggag cacaccggcg
     1081 tgagcgcgga ggaccgctac taggacgccg ccgtcggctg gcctcccttc gcgttgaccg
     1141 tggtcacggg gaggccgagg ccggccacct tgcgcggcag gtccaggtgc atccagcgcg
     1201 agaccctggt gggcagcgtc gagatgatga tctcgtcgaa gccgtggacg ttgaccgcgt
     1261 cctcgatcgc cgccagcggc tccggcgcgc cgaccagggc ctcgaccggc gcacccgccg
     1321 cctcctcgag cagcggcacg gccagcgcca ccaccgactc cgcctcggtg ctctcctggt
     1381 cctccggatc gaccagctgg tgcagcccgt gggccgacgc gggcaccagc agcgtgaacg
     1441 tggcctgccc gtgcgcggcc cgttcgcgga ccgcctcgat cagcgccggc gtcgccgccg
     1501 tcttgttggc caccacgagc acgcgcgtgg ccgctccggc ttccatacgt ccccttccct
     1561 cgcttgtgcc gattgtgcca agctggcggg gcggcggcaa ctagggccgg cggggcagcc
     1621 tgaccgtgcg cttgtagcgg gtcacgacct tggcgccgtc gggcttgatc gtgatccgca
     1681 gggccagctt gcgcttgcgt ctgaggacct tgcgcgtcgc cttgttcagc ttgaccttga
     1741 ccctgccggt cctgcccggc ctcaccgagt agcggctccg gccggccagc accagcttcg
     1801 cggcgctctt gcccgcggtg ccgggcacct cgatcagcag gaacccccca caggtgaccg
     1861 gcgaccccgg cgggcagccg acgcggatga acaccgcgcc atcggcccgg accacgggcg
     1921 ccgagccgct gggcagggcg atcggtgcgg cggccttgtt cggcgggggc gtgcccgccg
     1981 gcgaggacac gtagccgacg gggccgggcg gcggcggcga gtacggcggc gggtcg
//
LOCUS       DASUPW010000341         7033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_497447, whole genome shotgun sequence.
ACCESSION   DASUPW010000341 DASUPW010000000
VERSION     DASUPW010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7033
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497447"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1186
                     /locus_tag="VFY44_07660"
     CDS             176..1186
                     /locus_tag="VFY44_07660"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX5910354.1"
                     /translation="MNKRVLTAAVIACCLGGPATVAAQVPTPPPAPVPVPAPKVVKPG
                     VTVSGIPLEGLTQVQAVQRLREQLRPRAQRRFKVRVGRRSFTLTTGRLRMRVGEKATA
                     KRALKARAGAKVAPVITFRAASLSAFAQRTEQVTRRYGRNASMRITVTRIVGRKARYG
                     SRINAARLDKAIRRRLADPALARGVAPKVFRVRPAVRLIDLRRRNRTILTIDRAGHRL
                     RLFKNLRYDRSYGIAVGAAGTETPPGLYRIQNKQVNPAWTAPNRPWAGSYAGRTVPGG
                     APDNPLKARWLGIAGGVGIHGTAEAWSIGSNASHGCIRMRVPEVIDLFGRVPVGAQVL
                     IR"
     gene            complement(1222..1662)
                     /locus_tag="VFY44_07665"
     CDS             complement(1222..1662)
                     /locus_tag="VFY44_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910355.1"
                     /translation="MRAAAALAAAVALALPAAAPGAKPARTYSGQSSAGTAVGFKVST
                     GYVRGFAIGYVAECDDGETLRGTFRFKPAKVTDGRWAVSGPGSGRLPDGRTTASKLRL
                     SGELAGRRARGAFSITTQMARPDGNGVATCRSGRVTWKASRPPA"
     gene            complement(1659..2606)
                     /locus_tag="VFY44_07670"
     CDS             complement(1659..2606)
                     /locus_tag="VFY44_07670"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX5910356.1"
                     /translation="MPEIPVFDHDAVLSAIDPLEAIDQVRRGFVEYAAGEWTMPSKVY
                     LQAPPAGDFRAMPALGAGLAILKWVTSFPGNPGSGLPVVMGTISISSAADGRPLALAD
                     VRSVTALRTGAVAAVATQELAREDAATVGIVGCGLHGAWAARCLAAAEYGPGVCFDPD
                     PEAAGRLAGELGWEAGSLDDALGCDVVTCITPGNEPVVTAGALRPGVHLNMLGADGPG
                     KAEAEVAAVQRCTLFCDEWNQASHGGELTGAVEAGLEVRDQVTELGAVLTGRAPGRGS
                     DADMTLFDSTGLAIQDLALCHALMEAHSAGVLGARTVAL"
     gene            2701..4137
                     /locus_tag="VFY44_07675"
     CDS             2701..4137
                     /locus_tag="VFY44_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910357.1"
                     /translation="MNFDLHKLLATLAVAAPLALAGTANAAEPFGSDLTQTHRASNVD
                     CASYPCTLAQDFFEGWDSGYVRSTGVITGWKVKLNAGAKARLKVIREGDWNQFTATGT
                     GPSVNGTGGISSVATRIPVSYGDYAGVELQSGLLQTDFYGSDSTTQEMRFQGAFNDGQ
                     TREPGSQPIGHELMTQVTVEPDADEDGYGDETQDGCVFCNTGAGGGEVLPPSSSGGSS
                     SYSPGRGSGSSSSSDSSYGANRPLMKLAIDKRGFFEYRNGTPYITLYMDNQGRKNLEG
                     SIELKYGKKALSEVNKGVNKKFDIDWDDQKSLSNFKLPRKLVKQIERKGAVTLTATAR
                     MEAPDGARTTTKQTVKVRKAGLTNAYDGYYKGTGGLVIQVSGGYLTSISVGLNAYCQR
                     DAKFMMRSLYTLDGYPLMIGRDGSFKASGSQSPDTVKYEGKLTRKGTGKGYLSLFHTK
                     LDLGDGGRLQIDQCFAASNWKVQKVKGK"
     gene            4192..4953
                     /locus_tag="VFY44_07680"
     CDS             4192..4953
                     /locus_tag="VFY44_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910358.1"
                     /translation="MTSSIRRLLPVVCVAAPLVLTATATAKPAFEVGSRGIQSGKYAT
                     VYVNNEGGDENVQGTMTVRSGSKALKTNAIDLEYGDDYSYGNAVIPRRQLRELDRRGR
                     TKLTVVASVRGARTGQAQSIRKAVTIYSRGKTTAYDGTYKGTGGLVIDVQGGFLRSIN
                     VGVNMFCSRTKEFKQGSLYTLSGFPAMIGRDGSFRAKGTQSPNVIRYEGKLSRSGSAK
                     GYLSLFETDLVFGDGGTMQVQQCLGASNWKAKRTR"
     gene            5030..5275
                     /locus_tag="VFY44_07685"
     CDS             5030..5275
                     /locus_tag="VFY44_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910359.1"
                     /translation="MGTNGDQHPPRPRIQIAQAAASPEEAAAIAAALEQFLRDTAPAP
                     VAASTAVNPWLRAGLYENAGLDPNGPSPWGDDVAWGR"
     gene            5431..6795
                     /locus_tag="VFY44_07690"
     CDS             5431..6795
                     /locus_tag="VFY44_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910360.1"
                     /translation="MLAVSVMIACSAATVATVALASPDERKPPNHKKGYEVWTIDQSD
                     TRADGGGNLYVYDGPELADEPGSAVPEKIDLGGEARDMCLEQTGSAPRRPHMVVFNGG
                     NSSVDGNARAAIAFVVSGHVLFMDADERKPIKCIDTGDQAHAVWPTPDQRQIIVANQN
                     DKKLGRIATDYDSDEYELEPAATLDLANGTTPSGAPRQSPALRPDNAPICPRTDDEGK
                     RTFVTLRGGGMFVVDHRRTPMRIVAEYDKANVDENGCGEIQAGDKMYVNSGAGALATN
                     PDNHDVYAFDVDEFGGQPNPPNVPAPKLVYSYDGRTGVDSHGVLLTKGERYLWTGDRQ
                     ANTISVVDSRRDELVNEFSIAQSSSSDPAPDLMDISPDGKTVFASLRGPFPQSGGHAS
                     VGNTPGVGVIDVRRGGRDGRMDGVAKISNPTSSVESADPHAIRIRVLGGGPGREHDEH
                     PHGR"
     gene            6829..>7033
                     /locus_tag="VFY44_07695"
     CDS             6829..>7033
                     /locus_tag="VFY44_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910361.1"
                     /translation="MGRLLRSRRLQVSVALAVAAAACAYAASKTSGDELAGAYRESGH
                     ALLATAATHDLPGAARAAARPAAL"
BASE COUNT         1109 a         2582 c         2499 g          843 t
ORIGIN      
        1 gccaggctca cgtcgccgtt ccgggcgtag cggatgtccg ggtcacccat ggcgacgagt
       61 ctcgccatgg gggcaccgga gcgcaagcgg ctcgacctcg gtcctccccg aggcggattg
      121 ggcgggcgct ttcgcgtgcc cacgcggcgg cggccgcgcc ctactctggg cggtgatgaa
      181 caagcgcgtg ctgacggctg ccgtgatcgc ctgttgcctg ggagggcccg ccaccgtggc
      241 ggcgcaggtg cccacgccgc cgccggcgcc cgtgccggtg cccgcaccca aggtggtcaa
      301 gcccggcgtc accgtctcgg ggatcccgct cgaggggctc acgcaggtcc aggcggtcca
      361 gcggctgcgc gagcagttgc gcccacgggc ccagcgccgc ttcaaggtca gggtcggccg
      421 gcgctcgttc acgctgacca ccggccgcct gcgcatgcgc gtcggcgaga aggccacggc
      481 caagcgggcg ctcaaggcca gggccggcgc caaggtggcc ccggtgatca cgttccgcgc
      541 ggcatcgctg tccgcgtttg cccagcgcac ggagcaggtg acccggcgct acgggcgcaa
      601 cgccagcatg cgcatcaccg tgacccggat cgtcggccgc aaggcgcgct acgggtcgag
      661 gatcaacgcg gcgcggctgg acaaggcgat ccggcgccgg ctggccgacc cggccctggc
      721 ccgcggcgtg gcgccgaagg tgttccgcgt gcggccggcc gtccggctga tcgacctgcg
      781 ccggcgcaac cgcacgatcc tgaccatcga ccgcgccggc caccggctgc gcctgttcaa
      841 gaacctccgc tacgaccgct cgtacgggat cgcggtcggc gccgccggca cggagacccc
      901 gcccgggctg taccggatcc agaacaagca ggtgaacccg gcgtggaccg cgcccaaccg
      961 gccctgggcg ggcagctacg ccgggcgcac ggtgcccggc ggcgcgccgg acaacccgct
     1021 caaggcgcgc tggctgggga tcgccggcgg cgtcggcatc cacggcaccg ccgaggcgtg
     1081 gtccatcggc tccaacgcct cacacggctg catccgcatg cgcgtgcccg aggtgatcga
     1141 cctgttcggc cgcgtgcccg tgggcgcgca ggtcctcatt cgatgagctc cgggggctca
     1201 cgcccccggt cccccgcgac cctaggccgg tggtcttgag gccttccacg tgacccggcc
     1261 gctgcggcag gtggccacgc cgttgccgtc gggccgggcc atctgggtcg tgatcgagaa
     1321 ggcgcccctg gcccggcgcc cggccagctc gccgctgaga cgcagcttgg acgcggtcgt
     1381 gcggccgtcg ggcagcctgc cgctcccggg accgctgacc gcccagcggc cgtccgtgac
     1441 cttggcgggc ttgaagcgga acgtgccgcg cagcgtctcg ccgtcgtcgc actccgccac
     1501 gtagccgatg gcgaagccgc gcacgtagcc ggtcgagacc ttgaagccga cggcggtgcc
     1561 ggccgagctc tggccggagt aggtgcgggc gggcttggcg ccgggggcgg ccgcgggcag
     1621 cgccagcgcg accgccgccg cgagcgcggc cgccgccctc acagggccac cgtcctcgct
     1681 ccgaggacgc ccgcggagtg ggcctccatg agcgcgtggc agagcgccag gtcctggatg
     1741 gccagcccgg tcgagtcgaa cagggtcatg tcggcgtccg agccgcggcc cggggcgcgg
     1801 ccggtcagca cggcgccgag ctcggtgact tggtcgcgga cctccagccc ggcctccacg
     1861 gcgcccgtca gctcgccgcc gtgcgaggcc tggttccact cgtcgcagaa gagcgtgcag
     1921 cgctggacgg ccgcgacctc cgcctcggcc ttgccggggc cgtccgcgcc cagcatgttc
     1981 aggtgcaccc ccgggcgcag cgcgcccgcc gtgaccaccg gctcgttgcc gggcgtgatg
     2041 caggtcacga cgtcgcagcc cagcgcgtcg tccagcgatc ccgcctccca gcccagctcg
     2101 ccggccaggc ggcccgcggc ctccgggtcg gggtcgaagc acacgccggg gccgtactcg
     2161 gcggccgcca ggcagcgtgc cgcccacgcg ccgtgtagcc cgcagccgac gatgccgacg
     2221 gtggcggcgt cctcgcgggc cagctcctgg gtggccaccg ccgccacggc gccggtgcgt
     2281 agcgcggtga ccgagcgcac gtcggccagc gcaagcggcc gcccgtcggc ggcgctggag
     2341 atggagatcg tgcccatgac caccggcagg cccgagccgg ggttcccggg gaaggaggtg
     2401 acccacttga ggatggccag gccggcgccc agggcgggca tggcgcggaa gtcgcccgcc
     2461 ggcggcgcct ggaggtagac cttcgagggc atcgtccact cgcccgcggc gtattccacg
     2521 aagccgcggc gcacttggtc gatcgcctcg agcgggtcga tggccgagag cacggcgtcg
     2581 tggtcgaaga cggggatctc gggcaccggc cgaggatacc cgggggtcat cctcaggtcg
     2641 gaccccaagt tcggcctcag gcatgacgcc ggggccgcgc gccgcgtgga ggcttggcgc
     2701 atgaacttcg acctccacaa gctcctggca accctggccg tggccgcgcc gctggcgctg
     2761 gccggcacgg cgaacgcggc cgagcccttc ggcagcgacc tgacccagac ccaccgcgcc
     2821 tcgaacgtgg actgcgccag ctacccgtgc acgctggccc aggacttctt cgagggctgg
     2881 gactccggct acgtccgctc cacgggcgtg atcaccggat ggaaggtcaa gctgaacgcc
     2941 ggcgccaagg cgcgcctgaa ggtcatccgc gagggcgact ggaaccagtt caccgccacc
     3001 ggcaccggcc cctcggtgaa cggcacgggc ggcatcagct ccgtggccac gcgcatcccc
     3061 gtgtcctacg gcgactacgc cggggtggaa ctgcagtccg ggctgctgca gaccgacttc
     3121 tacggcagcg actcgaccac ccaggagatg cgcttccagg gcgcgttcaa cgacggccag
     3181 acacgcgagc cgggcagcca gcccatcggc cacgagctga tgacccaggt cacggtggag
     3241 cccgacgccg acgaggacgg ctacggcgac gagacccagg acggctgcgt gttctgcaac
     3301 accggcgccg gcggtggcga ggtgctgccc ccgtccagca gcggcggctc gtcctcgtac
     3361 tcgcccgggc gcggatccgg ctcgtccagc tcgagcgact ccagctacgg cgccaaccgg
     3421 ccgctgatga agctggcgat cgacaagcgc ggcttcttcg agtaccgcaa cggcaccccc
     3481 tacatcacgc tgtacatgga caaccagggc cgcaagaacc tggagggcag catcgagctg
     3541 aagtacggca agaaggccct gtccgaggtg aacaagggcg tgaacaagaa gttcgacatc
     3601 gactgggacg accagaagag cctgagcaac ttcaagctgc ccaggaagct ggtcaagcag
     3661 atcgagcgca agggcgccgt gacgctgacc gcgacggcca ggatggaggc ccccgacggc
     3721 gccaggacca ccaccaagca gaccgtgaag gtccgcaagg ccggcctgac caacgcctac
     3781 gacggctact acaagggcac cggcggcctg gtgatccagg tcagcggcgg ctacctgacg
     3841 tcgatcagcg tgggcctgaa cgcctactgc cagcgcgacg ccaagttcat gatgcgcagc
     3901 ctgtacacgc tggacggcta ccccctgatg atcggccgcg acggcagctt caaggccagc
     3961 ggctcgcagt cgcccgacac cgtgaagtac gagggcaagc tgacccggaa gggcaccggc
     4021 aagggctacc tgtcgctgtt ccacaccaag ctggacctcg gcgacggcgg caggctgcag
     4081 atcgaccagt gcttcgccgc cagcaactgg aaggtccaga aggtcaaggg caagtagcgc
     4141 gctagggaca tccccggtgc gcacggggag gggcccgcgg acgatggagc catgacttca
     4201 tcgatccgca ggctcctccc cgtcgtgtgc gtggcagcgc ccctggtgct gaccgcgacc
     4261 gccaccgcca agcccgcctt cgaggtcggc tcgcgcggca tccagtcggg caagtacgcc
     4321 acggtctacg tgaacaacga gggcggcgac gagaacgtcc agggcacgat gaccgtcagg
     4381 agcggctcga aggcgctgaa gacgaacgcg atcgacctgg agtacggcga cgactactcc
     4441 tacggcaacg cggtcatccc ccggcgccag ctccgggagc tggaccgcag gggccgcacc
     4501 aagctgaccg tggtcgccag cgtgcgcggc gcccggaccg gccaggcgca gagcatccgg
     4561 aaggccgtga ccatctacag ccgcgggaag accaccgcct acgacggcac ctacaagggc
     4621 accggcggcc tggtgatcga cgtccagggc ggcttcctgc gatcgatcaa cgtgggcgtg
     4681 aacatgttct gcagccgcac gaaggagttc aagcagggga gcctctacac gctctccggc
     4741 ttcccggcga tgatcggcag ggacggcagc ttccgggcca agggcaccca gtcgcccaac
     4801 gtcatccgct acgagggcaa gctgagccgg agcggcagcg ccaagggcta cctctcgctg
     4861 ttcgagaccg acctggtgtt cggcgacggc ggcaccatgc aggtgcagca gtgcctgggc
     4921 gccagcaact ggaaggcgaa acgcacgcgg tagcccttgc ggccatccat ggccgcgcct
     4981 cgaggtccca gctgccctcc gggcagcgat gtcctaggct cccgccccga tgggcaccaa
     5041 cggcgaccag caccccccgc ggccgcggat ccagatcgcg caggcggcgg cgtcccccga
     5101 ggaggccgcg gcgatcgccg cggcgctgga gcagttcctg cgcgacaccg cgccggcgcc
     5161 ggtggccgcc agcacggccg tgaacccgtg gctgcgggcc gggctgtacg agaacgccgg
     5221 gctcgatccc aacggcccgt cgccgtgggg cgacgacgtg gcctggggcc gctgagcggc
     5281 ggtaccgaaa ggttccatcg acacgcctcc ggccggcgcg tcgagcgaaa cgtctaggcc
     5341 tgcaaatcgg gctcaagtag tggcctcagc ggccgatgca cgggtatccc gccgaacgcc
     5401 gattgaaggg tcccaatgcg ccagaggttg atgctcgccg tgtccgtgat gatcgcctgc
     5461 tcggcggcca cggtcgccac cgtcgccctg gcctccccgg acgagcgcaa gccgccgaac
     5521 cacaagaagg ggtacgaggt gtggaccatc gaccagtccg acacgcgggc cgacggcggc
     5581 gggaacctgt acgtgtacga cgggcccgag ctggccgacg agcccggctc ggccgtgccc
     5641 gagaagatcg acctcggcgg cgaggcccgc gacatgtgcc tggagcagac cggtagcgcg
     5701 ccgcggcgac cgcacatggt ggtcttcaac ggaggcaaca gctccgtgga cggcaacgcc
     5761 cgcgccgcga tcgccttcgt ggtcagcggc cacgtgctgt tcatggacgc cgacgagcgc
     5821 aagccgatca agtgcatcga caccggcgac caggcgcacg ccgtgtggcc gacgcccgac
     5881 cagcgccaga tcatcgtggc caaccagaac gacaagaagc tggggcggat cgcgaccgac
     5941 tacgacagcg acgagtacga gctggagccg gcggccaccc tggacctggc caacgggacc
     6001 acccccagcg gcgctccccg ccagagcccg gccctgcggc ccgacaacgc tcccatctgc
     6061 ccgcgcacgg acgacgaggg caagcgcacc ttcgtgaccc tgcgcggcgg ggggatgttc
     6121 gtggtcgacc accgccggac cccgatgcgg atcgtggccg agtacgacaa ggccaacgtg
     6181 gacgagaacg gctgcggcga gatccaggcc ggcgacaaga tgtacgtgaa ctccggcgcc
     6241 ggagcgctgg ccaccaaccc ggacaaccac gacgtgtacg cgttcgacgt cgacgagttc
     6301 ggcggccagc ccaacccgcc gaacgtgccg gcgcccaagc tggtctacag ctacgacggc
     6361 cgcacgggcg tggactccca cggcgtgctc ctgaccaagg gtgagcggta cctgtggacc
     6421 ggcgaccgcc aggccaacac catctcggtg gtcgacagcc gtcgcgacga gctggtcaac
     6481 gagttcagca tcgcccagag ctcgtccagc gacccggctc ccgacctgat ggacatctcg
     6541 cccgacggca agacggtgtt cgcctccctg cgcggcccct tcccgcagtc cggcggccac
     6601 gcctcggtgg gcaacacccc cggggtcggc gtgatcgacg tgcggcgtgg tggccgcgac
     6661 ggccggatgg acggggtcgc caagatctcc aaccccacca gcagcgtcga gtcggccgac
     6721 ccgcacgcca tccgcatccg cgtgctgggc ggcggaccgg gccgcgaaca cgacgagcac
     6781 ccgcacggta ggtaggtgac cgccgctcca ccaccgcgtg gggggcggtt gggccggctg
     6841 cttcgcagcc gtcgcctgca ggtcagcgtc gcgctggccg tcgccgcggc cgcctgcgcc
     6901 tacgcggcca gcaagacgtc gggcgacgag ctggccggcg cgtaccgcga gtcgggccac
     6961 gccctgctgg ccaccgccgc cacgcacgac ctgcccggcg ccgcccgggc ggcggcgcgc
     7021 cccgcggcgc tgg
//
LOCUS       DASUPW010000342         4107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1882577, whole genome shotgun sequence.
ACCESSION   DASUPW010000342 DASUPW010000000
VERSION     DASUPW010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1882577"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /gene="purB"
                     /locus_tag="VFY44_07700"
     CDS             complement(<1..1079)
                     /gene="purB"
                     /locus_tag="VFY44_07700"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX5910362.1"
                     /translation="MIGRYTRPEMGAVWSEQRKLENWLAVELAVTDALAETGVVPAAD
                     AETIRANAAFTVEAVKEREQVTDHDVAAFVDVVAASVGEAGRWVHHGLTSSDVLDTAT
                     GLQIAQSGAILVAGQRAFRDALAGRAREHVDTLCVGRTHGVHAEPTTFGVKLAGCALE
                     AQRNLERLERAVAGASVGALSGAVGTYAANGPELEAVVLERLGLEREDVSTQVVARDR
                     HAELLSAVALAGAGLERFATEIRHLQRTEVREVEEPFRAGQKGSSAMPHKRNPITTER
                     LTGIARLLRGYAQSGLENVALWHERDISHSSVERVALPDATILLDYGLSLGVRVVEGM
                     TVHTDRMRANLDLTHGALFSQRALLA"
     gene            complement(1129..1590)
                     /gene="purE"
                     /locus_tag="VFY44_07705"
     CDS             complement(1129..1590)
                     /gene="purE"
                     /locus_tag="VFY44_07705"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX5910363.1"
                     /translation="MTEPATQTDTVTELTDIEGDAPLVGIVMGSKSDWDTMAKAAAEL
                     EERGIKHEMQVMSAHRDPDVVADYSKNARARGLKVVIAGAGLAAALPGTVAAHTDLPV
                     IGVPLQSRTSVAGGLDAMLAIAQMPPGVPVACVGVDGAKNAAVLAARILFA"
     gene            complement(1587..2867)
                     /gene="purD"
                     /locus_tag="VFY44_07710"
     CDS             complement(1587..2867)
                     /gene="purD"
                     /locus_tag="VFY44_07710"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX5910364.1"
                     /translation="MPTVLVVGAGGREHALVRRLLRSPRKPDVLAAPGNAGIARDKVE
                     CVPVEVEDIAGLVELARSREVDLVVVGPEVPLVLGLVDALQEAGIPAFGPSKEAARLE
                     GSKRYAKELMEAVGVPTAGYEVLTSKDQALEAVGRYSYPLVLKADSLAAGKGVIICPD
                     EASALEAIDTFFTEHRFGDTEVVLEEFLDGEELSLLAVCDGERAVAMAPAQDYKRIFD
                     GDEGPNTGGMGSYSPVPGIDRDHADALARAVHQPIVDEMKRRGTPFHGILYAGLMMTA
                     DGPKVLEYNCRFGDPETQAVLTRLRSDILDLLEGSTRTGGLEGVGIEWSDEWAVTVVL
                     ASRGYPESSSKGDEIRGLRSIDSIEVEVLHAGTAEVDGQIVTAGGRVLNVTGIGATPG
                     EAREHAYAAARRVQFDGKQMRTDIAARAVDRVEA"
     gene            complement(2871..3152)
                     /locus_tag="VFY44_07715"
     CDS             complement(2871..3152)
                     /locus_tag="VFY44_07715"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX5910365.1"
                     /translation="MPTYVLLSTLSPEGVQTIKNNPQRIREVNNEVEQLGAKVTAQWA
                     TLGRFDFVNIVEAPDETTMARVSLELGSRGTSHYESLTAIPVDEFIASL"
     gene            3362..>4107
                     /locus_tag="VFY44_07720"
     CDS             3362..>4107
                     /locus_tag="VFY44_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910366.1"
                     /translation="MPASANAATLAPGKPCFGDGDPITLGGTGFTPGAPVTVAADGRQ
                     FNGALTSTATGTIAGRLLPFSLVAGRTQNSVFTATDTANPANVGQTTVVRARLRVTVS
                     PANASPRSNRRFRARGFTAGTTLYRHIVRGRAVSNGKMKRLTGACRSTSVKKRLFRKS
                     AKSGTYRVQFDTKRKYSSKTLQRVRFRVRVYRIVRRASSASAGAATLAPGKPCFGDGD
                     PITLGGTGFTPGAPVTVAADGRQFNGALTST"
BASE COUNT          554 a         1550 c         1437 g          566 t
ORIGIN      
        1 gccagcaggg cccgctggct gaacagggcg ccgtgggtca ggtccaggtt ggcgcgcatc
       61 cgatcggtgt ggacggtcat gccctcgacc acccgcacgc ccagcgacaa cccgtagtcc
      121 aggaggatcg tggcgtcggg cagggcgacc cgctcgaccg agctgtgcga gatgtcgcgc
      181 tcgtgccaca gcgccacgtt ctccagcccc gactgggcgt agccgcgcag cagccgggcg
      241 atgccggtca ggcgctcggt cgtgatcggg ttgcgcttgt ggggcatggc cgacgagccc
      301 ttctggcccg cgcggaacgg ctcctcgacc tcgcgcacct cggtgcgctg gaggtggcgg
      361 atctcggtgg cgaagcgctc gagccccgcg ccggccagcg ccaccgcgct cagcagctcg
      421 gcgtggcggt cgcgggccac gacctgcgtc gacacgtcct cgcgctccag gcccaggcgc
      481 tcgagcacca cggcctccag ctcggggccg ttggccgcgt aggtgcccac ggcgcccgac
      541 agcgcgccta ccgaggctcc ggccacggcg cgctccaggc gctcgaggtt gcgctgggcc
      601 tccagcgcgc agcccgccag cttgaccccg aacgtggtgg gctcggcgtg cacgccgtgg
      661 gtgcgcccca cgcacagggt gtccacgtgc tcgcgcgcgc ggcccgccag ggcgtcgcgg
      721 aacgcgcgct ggccggccac gaggatcgcg cccgactggg cgatctggag ccccgtcgcg
      781 gtgtccagga cgtcggacga ggtcagcccg tggtggaccc agcgaccggc ctcgccgacc
      841 gaggcggcca ccacgtccac gaacgcggcc acgtcgtggt cggtgacctg ctcgcgctcc
      901 ttgaccgcct cgaccgtgaa ggccgcgttg gcccggatgg tctcggcgtc ggccgcgggc
      961 accacgcccg tctcggccag ggcgtcggtg acggccagct cgacggcgag ccagttctca
     1021 agcttgcgct gctcggacca caccgcgccc atctcgggcc gggtgtagcg cccgatcacg
     1081 cgagaggacc ggaggcggac gtcgtccgcc gaggaccgat cgacaccatc acgcgaagag
     1141 gatgcgcgcc gcgaggacgg cggcgttctt ggcgccgtcc acgccgacgc aggccacggg
     1201 cacgccgggg ggcatctggg cgatggccag catcgcgtcc agcccgccgg ccaccgaggt
     1261 gcgcgactgc agcggcacgc cgatcacggg caggtcggtg tgcgcggcca ccgtgcccgg
     1321 cagggccgcg gccaggccgg cgccggcgat gaccaccttg aggccgcggg cgcgggcgtt
     1381 cttggagtag tcggccacca cgtcgggatc gcggtgggcg ctcatgacct gcatctcgtg
     1441 cttgatgccc cgctcctcga gctccgcggc cgccttggcc atggtgtccc agtcggactt
     1501 cgagcccatg acgatgccga ccagcggcgc gtctccctcg atgtccgtta gctcggtgac
     1561 ggtgtcggtc tgggtggcgg gctcggtcat gcctcgaccc tatcgacggc ccgcgctgcg
     1621 atgtcggtac gcatctgctt gccgtcgaac tgcacccggc gggcggcggc gtaggcgtgc
     1681 tcgcgcgcct cgcccggcgt ggcgccgatc ccggtcacgt tcagcacccg gccccctgcg
     1741 gtcacgatct ggccgtccac ctcggcggta ccggcgtgca gcacctcgac ctcgatcgag
     1801 tcgatcgagc gcaggccgcg gatctcgtcg cccttggacg aggactccgg atacccgcgc
     1861 gaggccagca ccacggtcac ggcccactcg tccgaccact cgatgccgac gccctccagc
     1921 ccgccggtgc gggtggagcc ctcgagcagg tccaggatgt ccgagcgcag ccgggtcagc
     1981 accgcctggg tctcggggtc gccgaagcgg cagttgtact ccaggacctt gggcccgtcc
     2041 gccgtcatca tcagcccggc gtagaggatc ccgtggaagg gggtgccgcg gcgcttcatc
     2101 tcgtcgacga tcggctggtg gacggcccgc gccagcgcgt cggcgtggtc gcggtcgatc
     2161 ccggggacgg gcgagtagct gcccatgccg ccggtgttcg gcccctcgtc gccgtcgaag
     2221 atccgcttgt agtcctgggc cggggccatg gccacggcgc gctcgccgtc gcacacggcc
     2281 agcagcgaca gctcctcgcc gtccaggaac tcctcgagca ccacctcggt gtcaccgaac
     2341 cggtgctcgg tgaagaaggt gtcgatggcc tcgagcgccg aggcctcgtc ggggcagatg
     2401 atcacgccct tgcccgcggc cagcgagtcg gccttgagca ccagcgggta ggagtaccgg
     2461 ccgaccgcct ccagggcctg gtccttggag gtcagaacct cgtagccggc ggtgggcacg
     2521 cccacggcct ccatcagctc cttggcgtag cgcttggacc cctccagccg agcggcctcc
     2581 ttcgacgggc cgaaggcggg gatcccggct tcctggagcg cgtcgaccag gcccagcacc
     2641 agcggcacct cgggccccac caccaccagg tcgacctcgc gcgagcgcgc cagctccacc
     2701 agcccggcga tgtcctcgac ctccaccggc acgcactcga ccttgtcgcg cgcgatcccg
     2761 gcgttccccg gcgcggccag cacgtcgggc ttgcgtggcg aacgcagcag ccggcgcacg
     2821 agcgcgtgct cgcggccgcc ggctccaact acgaggacgg tcggcaacgg ctacagcgag
     2881 gcgatgaact cgtccacggg gatggccgtg agcgactcgt agtggctggt cccgcgggag
     2941 cccagctcca gcgacacgcg cgccatggtc gtctcgtcgg gcgcctccac gatgttcacg
     3001 aagtcgaacc ggcccagggt ggcccactgc gcggtgacct tggcccccag ctgctcgacc
     3061 tcgttgttga cctcgcggat gcgctgcggg ttgttcttga tggtctgcac gccctcgggg
     3121 gagagggtgc tgagcagcac gtaagtgggc acgaacgtcc tttctctcgg ttccctgctg
     3181 cgccggcggc gcaccgaagg ttctcgcagt ttttccgcac cgacgcaacc cgcccggggt
     3241 tacgtctgcg taggaaccgc gcgcgccggg gaaactggcc cgtgaccacc aacctgggag
     3301 caaccacatg aagatgcgac ggatcgcagt cccggccctc ttggcgacgg ccgtctgcgc
     3361 ggtgccggcg tcggcgaacg cggcgacgct ggcccccggc aagccctgct tcggcgacgg
     3421 ggacccgatc accctgggcg gcaccggctt cacgcccggc gcgcccgtga ccgtggccgc
     3481 cgacgggcgt cagttcaacg gcgcgctgac ctccaccgcc accgggacga tcgccggccg
     3541 cctcctgccg ttcagtctgg tcgcgggccg gacccagaac tcggtcttca ccgccaccga
     3601 cacggccaac ccggccaacg tgggccagac gaccgtggtc cgcgcccgcc tgcgggtgac
     3661 ggtcagcccg gccaacgcca gcccgcgcag caaccgccgc ttccgggccc gtggcttcac
     3721 cgccggcacc acgctctacc gccacatcgt ccgcggcagg gccgtcagca acggcaagat
     3781 gaagcgcctg acaggggcct gccgctcgac cagcgtgaag aagcgcctgt tccgcaagag
     3841 cgccaagagc ggcacctacc gggtgcagtt cgacaccaag cgcaagtaca gctccaagac
     3901 gctgcagcgc gtgcgcttcc gcgtccgggt gtaccggatc gtgcgccggg ccagctcggc
     3961 ctcggccggc gcggccaccc tggcccccgg caagccgtgc ttcggcgacg gggacccgat
     4021 cacgctgggc ggcaccggct tcacccccgg cgcgcccgtg accgtggccg ccgacggtcg
     4081 ccagttcaac ggcgccctga cctcgac
//
LOCUS       DASUPW010000343         2534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_81660, whole genome shotgun sequence.
ACCESSION   DASUPW010000343 DASUPW010000000
VERSION     DASUPW010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81660"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..921)
                     /locus_tag="VFY44_07725"
     CDS             complement(70..921)
                     /locus_tag="VFY44_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910367.1"
                     /translation="MTVAGRLIGFSALLAVVFALAALAGAQADLGSSPPEPAHPGADA
                     GGHGERDPHAARVDEVRGLAVSRRGLTLELARTRVRAGRPVELAFRITDRQGRTVRRF
                     DVEHARRMHLILARRDLGAFQHLHPRQATDGSWSVRARLPEGGSYRLFADFSRGGRAQ
                     TLGADLGVTGPTRSRALPAPATIARAGDLTVTQRAPRARAGREAELEYAVTRAGRRVA
                     VEPYLGARGHLVALREGDLAFLHVHPDRDRLRFATRFPSAGRYRLFLQFQHEGRVQTV
                     AFTQEVS"
     gene            973..1263
                     /locus_tag="VFY44_07730"
     CDS             973..1263
                     /locus_tag="VFY44_07730"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX5910368.1"
                     /translation="MATTPTRGYSATKDQLLARLNRIEGQVRGVEKMVADERYCIDVL
                     TQIGAIQAALDKVALGLMDDHAKHCVIGAAEGEQDEKTEELMAAVGRLMRRS"
     gene            complement(1265..1347)
                     /locus_tag="VFY44_07735"
     tRNA            complement(1265..1347)
                     /locus_tag="VFY44_07735"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1312..1314),aa:Leu,seq:taa)
     gene            1425..>2534
                     /locus_tag="VFY44_07740"
     CDS             1425..>2534
                     /locus_tag="VFY44_07740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5910369.1"
                     /translation="MEASALRQQSALPHLGGRSPLLRLQSDEKLVAMIRRGNNSAFDA
                     LVQRYQPRLLSFCRHMLGSQEDAEDVLQEVFAASFNALLADDRPINARPWLYRIARNR
                     CLNHLRRPRASGQDSMDIFENENGASTAETVHRREEFRQIVADVQELPETQRTALLLR
                     EIDALSYDQIAEAMDTTVPSVKSLLVRARVGLAEASEARLLTCEEVRVELAEVAEGLS
                     RTTAPARRHLKNCERCRTFRSELKTTSKALAAVLPIGPLIFFKKLGIAHLGFGGGAAA
                     GGAAAGGAATGGAGLAAGGAAAGGAAAGGAISAGVGTLASKAAAGMAAAVIVTAGAVE
                     VNHVAKRNPAQTPAVAQAPAPPVAPAPVAVKPAPVA"
BASE COUNT          354 a          932 c          937 g          311 t
ORIGIN      
        1 aggcgcaggt catgccggtg atcggcagct cgacctgggc ggtggcggtg gcggtctgct
       61 cgggcatggc tacgagacct cctgggtgaa ggcgacggtc tggacgcggc cctcgtgctg
      121 gaactggagg aacagccggt agcggccggc cgaggggaag cgggtggcga agcgcaggcg
      181 gtcccggtcg gggtgcacgt gcaggaaggc caggtcgccc tcgcgcagcg ccaccagatg
      241 cccgcgcgcg ccaaggtagg gctcgaccgc gacccgccgc cccgcgcggg tgactgcgta
      301 ctccagttcg gcctcgcggc cggcccgggc ccggggggcg cgctgggtca cggtcaggtc
      361 accggcgcgg gcgatggtgg ccggagccgg gagcgcccgg ctccgggtgg gaccggtgac
      421 gcccaggtcc gcacccaggg tctgcgcccg cccgccgcgg ctgaagtcgg cgaacaggcg
      481 gtaggagccg ccttcgggca gccgggcgcg caccgaccac gagccgtcgg tcgcctggcg
      541 cgggtgcagg tgctggaagg cgcccaggtc gcgccgggcc aggatcaggt gcatgcgcct
      601 ggcgtgctcg acgtcgaagc ggcgcaccgt gcggccctgc cggtcggtga tgcggaaggc
      661 cagctccacc ggcctccccg cgcgcacgcg ggtgcgcgcg agctccaggg tcaggccgcg
      721 ccggctgacg gccaggccgc gcacctcgtc gacccgggcg gcgtgggggt cgcgctcccc
      781 gtggccgccg gcatctgcgc caggatgcgc cggctcgggc ggcgaggacc ccaggtcggc
      841 ctgcgcgccg gccagcgcgg ccagcgcgaa cacgacggcg aggagtgcgg agaagccgat
      901 caggcggcct gcgacggtca cgtgggccac cgtagcagat acccccaccc cgtatacgtg
      961 tacactcgct gcatggccac gacaccgacg cgcggctaca gcgcgaccaa ggaccagctg
     1021 ctcgcccgcc tgaaccgcat cgagggccag gtacggggag tcgagaagat ggtggcggac
     1081 gagcggtact gcatcgacgt cctgacccag atcggcgcca tccaggccgc cctggacaag
     1141 gtggccctcg gcctgatgga cgaccacgcc aagcactgcg tgatcggcgc cgccgagggc
     1201 gagcaggacg agaagaccga ggagctgatg gccgccgtcg gccgcctgat gcgccgcagc
     1261 tgagtgcccg gggtgggact cgaacccaca cgctcctgag agccgggact tttaaggccc
     1321 ctctgtctac cgttccagca cccgggcggg agtcagtcta ggccgcaact tggccctcgt
     1381 tagcgtgtct ttacgcctgg gaactaccgt ttccaggccc ttccatggaa gcttccgctc
     1441 tacgacagca gtccgcgctg ccccacctgg ggggccgttc gcctctcctg aggctgcaga
     1501 gcgacgagaa gctcgtggcc atgatccggc gcggcaacaa cagcgccttc gacgcgctgg
     1561 tccagcgcta ccagccgagg ctgctgtcct tctgccgcca catgctgggc tcccaggagg
     1621 acgccgagga cgtgctgcag gaggtcttcg cggcttcgtt caacgccctg ctggccgacg
     1681 accggcccat caacgcgcgc ccgtggctgt accggatcgc ccgcaaccgg tgcctgaacc
     1741 acctgcgccg cccgcgcgcg tcgggccagg actcgatgga catcttcgag aacgagaacg
     1801 gcgccagcac cgccgagacg gtccaccggc gcgaggagtt ccgccagatc gtggccgacg
     1861 tgcaggagct gcccgagacc cagcgcaccg ccctgctgct gcgcgagatc gacgcgctgt
     1921 cctacgacca gatcgccgag gcgatggaca ccacggtgcc cagcgtcaag tcgctgctgg
     1981 tccgcgcccg ggtcggcctg gccgaggcct ccgaggcccg cctgctgacc tgcgaggagg
     2041 tgcgcgtcga gctggccgag gtggccgagg ggctcagccg caccaccgcc ccggcgcgcc
     2101 gccacctcaa gaactgtgag cgctgccgca ccttccgcag cgagctgaag accacctcca
     2161 aggcgctggc cgccgtgctc cccatcggcc cgctgatctt cttcaagaag ctcggcatcg
     2221 cccacctggg cttcggcggc ggtgccgcgg ccggcggcgc ggccgccggt ggtgccgcca
     2281 cgggcggcgc gggcctcgcg gccggtggcg cagccgccgg cggcgccgcg gccggtggag
     2341 cgatctcggc cggcgtgggc accctggcct ccaaggcggc ggcgggcatg gccgccgcgg
     2401 tgatcgtcac cgcgggcgcc gtcgaggtca accacgtggc caagcgcaac ccggcccaga
     2461 cgcccgccgt ggcccaggcc ccggccccgc ccgtggctcc ggccccggtc gcggtcaagc
     2521 cggctccggt cgcc
//
LOCUS       DASUPW010000344         2331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1051341, whole genome shotgun sequence.
ACCESSION   DASUPW010000344 DASUPW010000000
VERSION     DASUPW010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051341"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VFY44_07745"
     CDS             complement(<1..705)
                     /locus_tag="VFY44_07745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5910370.1"
                     /translation="MSQGRLSDDRLARAAAHGEPDAFGEVFARYNGALYGYCLSILHD
                     PEDARDALQATMEKAFAAISTQRVKGGLRAWLFGIAHNEAISLARTRQATQPYGDELA
                     LAAAAGADPAERERMTQLVSDLKTLPEQQRSALVLRELSGLGSEEIGSALGISPSAAK
                     QAVYEARATLLAQSGGRDMDCRQVQMKLSDSDGRVRRGRRLKAHLADCALCTAFAAAI
                     PARTADMRMVFPPLGAA"
     gene            complement(737..1072)
                     /locus_tag="VFY44_07750"
     CDS             complement(737..1072)
                     /locus_tag="VFY44_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910371.1"
                     /translation="MNSQSALRRIVTLSALALALAPASALAQSSDEGYETDGPTVQNQ
                     IEGGGTAGQSESGTNPAPSSDSADSGDQAAGLPFTGLDLGLLAGAGVLLLGMGAGVRF
                     ALRLPPPVR"
     gene            complement(1134..>2331)
                     /locus_tag="VFY44_07755"
     CDS             complement(1134..>2331)
                     /locus_tag="VFY44_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5910372.1"
                     /translation="FRIGAVALPCTEQLRASDLRARMDAAEPVAVLCDRRNADTVRDA
                     GFAGTLLVVPDESLFEAAAAPAADLGERDPALLVFTSGTSGTPKPIRHGQRYLAGQHV
                     QAEHWFGARPGELAWCTAASGWSKSARNSFIAPWLRGATALLHDARFDPAERLELIER
                     ERVAVLCMAPTEYRVMAKRTELRAMPTLRHAVAAGEPLNPEIVHLWQDRVGVSVHDGY
                     GQTETGALTGMPLDRPVRPGSMGVALPGFDLWIDEGELCVDPATVPTFFLDGPEGPWR
                     TGDRVSRDDDGYLWFEGRTDDVIISAGYRIGPFEVESALVAHPDVAEAAAVAAPDEER
                     GQVVRAVVVLREGTRPGDELARALQEHVKEQTAPYKYPRIVEFADALPKTASGKIKRA
                     ELRRRD"
BASE COUNT          300 a          851 c          872 g          308 t
ORIGIN      
        1 ggccgccccc agcggcggga agaccatgcg catgtcggcg gtgcgggccg ggatggcggc
       61 ggcgaaggcg gtgcacaggg cgcagtcggc caggtgggcc ttgaggcgac gcccgcggcg
      121 cacgcggccg tccgagtcgg acagcttcat ctgaacctgg cggcagtcca tgtcacgacc
      181 tccgctctgg gcgaggaggg tggcccgggc ctcgtacacg gcctgcttgg cggcggaggg
      241 cgagatgccc agcgccgagc cgatctcctc cgagcccagg ccggacagct cgcgcagcac
      301 cagcgccgag cgctgctgct cgggcagcgt cttcaggtcg gacaccagct gggtcatgcg
      361 ctcgcgctcg gccgggtccg cgccggcggc cgccgccagc gccagctcgt cgccgtaggg
      421 ctgggtggcc tggcgcgtgc gggccagcga gatcgcctcg ttgtgcgcga tgccgaacag
      481 ccacgcgcga aggccgccct tcacccgctg cgtggagatg gccgcgaacg ccttctccat
      541 cgtggcctgg agcgcgtcgc gcgcgtcctc ggggtcgtgc aggatcgaca ggcagtaccc
      601 gtacagcgcg ccgttgtagc gcgcgaagac ctcgccgaac gcgtccggct cgccgtgcgc
      661 agcagcgcgg gcgagccggt cgtcgctgag ccgtccctgg ctcaagtgtc acctccgtag
      721 gggcgatggc tcgcgctcac cgcacggggg gaggaagtct caaggcgaag cgcacgcccg
      781 cgcccatgcc cagcagcagc acgccggcac cggccagcag gcccaggtcc aggccggtga
      841 acggcaggcc ggccgcctga tcgccggagt cggcggagtc gctcgaggga gcggggttgg
      901 tgccggactc gctctgcccg gccgtgccgc ccccctcgat ctggttctgg acggtcgggc
      961 cgtcggtctc gtagccctcg tcggagctct gggcgagtgc cgaggcgggg gccagcgcca
     1021 gggcgagggc ggacagggtc acgatgcggc gcagggcgga ttgcgagttc atgacccata
     1081 gacgcagcgt gagggcgaaa gtcagggcct tccaatttct tggcgcaaaa gcactagtcg
     1141 cgccggcgca gctccgcccg cttgatcttg ccgctggcgg tcttgggcag cgcgtcggcg
     1201 aactccacga tgcgtgggta cttgtacggc gcggtctgct ccttcacgtg ctcctgcagc
     1261 gcccgggcca gctcgtcgcc cggccgtgtg ccctcgcgca ggaccaccac cgcgcgcacc
     1321 acctggccac gttcctcgtc gggcgcggcc acggcggcgg cctcggccac gtcggggtgg
     1381 gccaccagcg ccgactccac ctcgaacggc ccgatccggt acccggccga gatgatcacg
     1441 tcgtcggtcc tgccctcgaa ccacaggtag ccgtcgtcgt cccggctgac gcggtcgccg
     1501 gtgcgccagg gcccctcggg gccgtccagg aagaaggtgg gcaccgtcgc gggatccacg
     1561 cacagctcgc cctcgtcgat ccacaggtcg aaccccggca gcgccacgcc catcgagccc
     1621 gggcggacgg gccggtccag cggcatgccg gtcagggcgc cggtctcggt ctggccgtac
     1681 ccgtcgtgca ccgacacgcc gacgcggtcc tgccacaggt gcacgatctc cgggttcagg
     1741 ggctcgccgg cggcgaccgc gtggcggagc gtcggcatcg cgcgcagctc ggtgcgcttg
     1801 gccatcaccc ggtactcggt gggggccatg cacagcacgg ccacccgctc gcgctcgatc
     1861 agctccaggc gctcggcggg atcgaagcgg gcgtcgtgga gcagggccgt cgcgccgcgc
     1921 agccacggtg cgatgaacga gttgcgcgcc gacttcgacc acccgctggc ggccgtgcac
     1981 caggccagct cgcccggccg ggcgccgaac cagtgctcgg cctgcacgtg ctggcccgcc
     2041 aggtagcgct ggccgtggcg gatcggcttc ggcgtccccg aggttcccga ggtgaacacc
     2101 agcagggccg ggtcgcgctc gcccaggtcg gccgccgggg cggctgcggc ctcgaacagg
     2161 ctttcgtcgg ggaccaccag cagcgtgccg gcgaagccgg cgtcgcgcac cgtgtcggcg
     2221 ttgcgccggt cgcagagcac cgcgaccggc tcggccgcgt ccatgcgggc gcgcaggtcg
     2281 ctcgcgcgca gctgttcggt gcacggcagg gcgaccgcgc cgatccggaa g
//
LOCUS       DASUPW010000345         2248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1883138, whole genome shotgun sequence.
ACCESSION   DASUPW010000345 DASUPW010000000
VERSION     DASUPW010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1883138"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1473)
                     /gene="glnA"
                     /locus_tag="VFY44_07760"
     CDS             complement(124..1473)
                     /gene="glnA"
                     /locus_tag="VFY44_07760"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX5910373.1"
                     /translation="MADQPTTAADVKALVEEQDIRFIRLWFTDILGQLKSFSINAAEL
                     DDAFEGGMGFDGSSITGFNAIEESDMIAMPDISTFRALPWRPEEAGVARMFCDVLTPS
                     REPYEGDPRHVLRRALERAESMGFDTFNVGPELEYFYFRNARPEHGQPEILDEGGYFD
                     LTTLDAGSDVRRDTVLALEELGMHVEYSHHEVGPSQHEIDMRYAPALKMADDCMTYRI
                     TVKEYAMKYGWHATFMPKPLFGENGSGMHTHMSLFKDGANAFYDADDQFYLSAVGKAF
                     IAGQLRHAREMSSVFAQWVNSYKRLVPGYEAPVYAAWSRRNRSALVRVPLYHPGKEQA
                     TRMELRCPDPACNPYLTFAILLNAGLEGIEKGYELPEPMEKNLYHLSPDERKRLGIEQ
                     LPETLGEAIELTAESELALRTFGEHIFNRFIEIKRQEWEDYRVQVSQWELDRYMSIL"
     gene            complement(1542..1805)
                     /locus_tag="VFY44_07765"
     CDS             complement(1542..1805)
                     /locus_tag="VFY44_07765"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5910374.1"
                     /translation="MVGKRWTGAILMVMMEGPVRFSEIRQLVPDLSDRLLSERLKELE
                     GENIVARRQDCDSPSCVRYELTEKGEALEPAVRALKRWAHDYR"
     gene            complement(1958..>2248)
                     /locus_tag="VFY44_07770"
     CDS             complement(1958..>2248)
                     /locus_tag="VFY44_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910375.1"
                     /translation="GRDAGLGIDEMALAREFDSHDETEATLLRYVKALLEHDGSPPLH
                     VHEAAREAGWEDEQILEAVAHVALNTFANLVTRAGDVPVDGSGEEARLLQAA"
BASE COUNT          339 a          766 c          771 g          372 t
ORIGIN      
        1 catgcccgcc agccccgccg cgtaggcggt ccgctccagg aaatcccgac gactgaggtc
       61 ctcctcacgc accctggggc caccctacgc caggtgtgcc gcggcgtgta gcggttccgt
      121 tacctagagg atcgacatgt agcggtccag ctcccactgg gagacctgga cgcggtagtc
      181 ctcccactcc tgccgcttga tctcgatgaa gcggttgaag atgtgctcgc cgaaggtgcg
      241 cagggccagc tcggactcgg ccgtgagctc gatcgcctcg ccgagggtct cgggcagctg
      301 ctcgatgccc agccgcttcc gctcgtcggg cgacaggtgg tagaggttct tctccatcgg
      361 ctcgggcagc tcatagccct tctcgatgcc ctccaggccc gcgttcagca ggatcgcgaa
      421 ggtcagatag gggttgcagg cggggtcggg gcagcgcagc tccatccgcg tggcctgctc
      481 cttgccgggg tggtagagcg gcacccgcac cagcgcggag cggttgcggc gcgaccaggc
      541 ggcgtagacc ggcgcctcgt agcccggcac caggcgcttg taggagttga cccactgggc
      601 gaacaccgag ctcatctcgc gcgcgtggcg cagctggccg gcgatgaagg ccttgcccac
      661 ggccgagagg tagaactggt cgtcggcgtc gtagaaggcg ttggcgccgt ccttgaacag
      721 cgacatgtgg gtgtgcatcc cggagccgtt ctcgccgaac agcggcttgg gcatgaaggt
      781 ggcgtgccag ccgtacttca tggcgtactc cttgaccgtg atgcggtagg tcatgcagtc
      841 gtcggccatc ttcagcgccg gggcgtagcg catgtcgatc tcgtgctggg agggcccgac
      901 ctcgtggtgg ctgtactcca cgtgcatgcc cagctcctcg agcgccagca cggtgtcgcg
      961 gcgcacgtcg gagccggcgt ccagcgtggt caggtcgaag tagccgccct cgtccaggat
     1021 ctcgggctgg ccgtgctcgg ggcgcgcgtt gcggaagtag aagtactcca gctcggggcc
     1081 cacgttgaag gtgtcgaagc ccatcgactc ggcccgctcc agcgcccgcc gcagcacatg
     1141 gcgcggatcg ccctcgtagg gctcacggct gggggtgagc acgtcgcaga acatgcgcgc
     1201 cacgccggcc tcctcgggcc gccagggcag tgcccggaag gtggagatgt cgggcatggc
     1261 gatcatgtcc gactcctcga tggcgttgaa gcccgtgatc gaggagccgt cgaagcccat
     1321 cccgccctcg aacgcgtcgt ccagctcggc cgcgttgatc gagaagctct tcagctggcc
     1381 caggatgtcg gtgaaccaga gccggatgaa gcggatgtcc tgctcctcga cgagggcctt
     1441 gacgtcggcg gcagtggtgg gctggtcggc catggacaga agatatcccc cggaggagtc
     1501 acggcccccg gaccccatgt cgaacttcgg cgcgcccggg tctaccggta gtcgtgcgcc
     1561 cagcgcttga gcgcgcggac cgcgggctcg agcgcctcac ccttctcggt gagctcgtag
     1621 cgcacgcagg acggcgagtc gcagtcctgg cggcgggcga cgatgttctc gccctcgagc
     1681 tccttcagcc gctcggagag cagccggtcg gacagatcgg ggaccagctg gcggatctcg
     1741 gagaaccgca ccgggccctc catcatcacc atcaggatcg cgccggtcca gcgctttccg
     1801 accatctcga tcgcacggtg gtagagggag cagcaggggg cccgatcgac atcgggctcc
     1861 tcgacacgaa cttctgtcgc cactccaccc ggggggtgca tggcctcaac tgtaaacgac
     1921 ccggcggagt acgccatcac ccgccgtttt gcagggatta tgcggcctgc agcaaccgcg
     1981 cttcctcgcc cgatccgtcc accgggacgt cccccgcgcg cgtgaccaga ttggcgaagg
     2041 tgttcagggc cacgtgcgcg acggcctcca ggatctgctc gtcctcccag ccggcctcgc
     2101 gggccgcctc gtgcacgtgc agcgggggcg aaccgtcgtg ctcgagcagc gccttcacgt
     2161 accgcagcag cgtggcctcg gtctcgtcgt gggagtcgaa ctcacgcgcc agggccatct
     2221 cgtcgatgcc caggcccgcg tcacggcc
//
LOCUS       DASUPW010000346         4231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_498258, whole genome shotgun sequence.
ACCESSION   DASUPW010000346 DASUPW010000000
VERSION     DASUPW010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498258"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..666
                     /locus_tag="VFY44_07775"
     CDS             70..666
                     /locus_tag="VFY44_07775"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX5910376.1"
                     /translation="MSVAVEPQHADLSVGPRVRALRDAMGLSLRELADRTGVSAPMLS
                     QVERGETSPTLVVATRIAAGLELTLSQLLRLDEGGSVSVVRAADRLKGGTGRRGHTYE
                     VVTPPLPGQRAEVAFHTLAPGASTGGPGDPPLHEPGSRETAVVTEGALRLLCDGQAHD
                     LQEGDAVTFDADLAHHFENPNDEDARFLAVVAAGLRRS"
     gene            667..2040
                     /gene="leuC"
                     /locus_tag="VFY44_07780"
     CDS             667..2040
                     /gene="leuC"
                     /locus_tag="VFY44_07780"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF004016.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX5910377.1"
                     /translation="MPQTLFDKIWDSHEVADALLYIDLHLVHEVTSPQAFESLRLQGR
                     KVRRPDRTLATADHNVPTDGATTAEQIKDVLSRKQVEALESNCKEFGVPVHSIGSKLQ
                     GIVHVIGPELGVTQPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLAQSKP
                     KSMRITFEGDEMPAGVTAKDLILATIGQMGVAGGTGHVIEFAGPVIEQLSMEGRMTIC
                     NMTIEGGGRAGMIAPDETTFEYLKGRPGAPQGADWDAAVEHWKTLHTDEGATYDKEVV
                     VDVASVSPMVTWGTTPGQVVGVSGTVPEPTSDQERRALEYMALEPGTPMHEIRPDRVF
                     IGSCTNSRIGDLREAAGVIEGRRVADGVIAMVVPGSAQVKEQAEAEGLDEVFRSAGFD
                     WRGAGCSMCLGMNPDVAAPGERVASTSNRNFEGRQGKGARSHLMSPRMAAAAAIEGHF
                     VDIRDWS"
     gene            2044..2607
                     /gene="leuD"
                     /locus_tag="VFY44_07785"
     CDS             2044..2607
                     /gene="leuD"
                     /locus_tag="VFY44_07785"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX5910378.1"
                     /translation="MEPIKQIQGGVSVLMRDDIDTDQIIPARYMKRVERTGFGEYLFA
                     DIKEKAGFDVPPNPILVTGVNFGCGSSREHAPWALDDYGFKVIIAKSFADIFRGNAPK
                     TGLLPVELAEDEVEFVAAQDDAVVDLESQEVRAGGRTFSFEFPAESKRRLLNGLDDIG
                     VTLDSEEKISAYEAERERKGPDTLTFA"
     gene            2611..3291
                     /locus_tag="VFY44_07790"
     CDS             2611..3291
                     /locus_tag="VFY44_07790"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5910379.1"
                     /translation="MAATAWFDRWATQYDGERRLLISPFDAFYGAAAEVASLGRTGEG
                     ARVLDLGAGTGLLTAALADALPGAHFTLLDEAPGMLAQARDRLAPLGDDAVAVVQGDL
                     IADLPEGPFDVIASALAIHHLPHEDQARLYALARERLAPGGVFLNAEQVAGPSPALED
                     HFVERELVHARAGGFTAEAEEQAWIRWSADRHVPVERQIEWLHQAGFATVECVYKAWR
                     FAVVAGWR"
     gene            3384..4217
                     /locus_tag="VFY44_07795"
     CDS             3384..4217
                     /locus_tag="VFY44_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910380.1"
                     /translation="MRSSTRAVLATALAIGAAALAPAAASAWAPADSATIHPGIQTET
                     AGSGQCTANFIFTRGSDTYIGQAAHCASTGAATDTDGCLAQSLPLGTKVTLVGSGATG
                     TLAYSSWLTMQGKGETDADACAYNDLALVKIDPADVSKVNPSVPGFGGPTGLGSPGGA
                     LSDVYSYGNSSLRGGITQLSPKQGKLIQTSGNGWNHTLYTLTPGIPGDSGSGFLSSTG
                     AAFGTLSTVAIAPLPASNGVGDLSKELAYANANGFSGTALANGTEPFTPNIVGAILGA
                     G"
BASE COUNT          648 a         1516 c         1506 g          561 t
ORIGIN      
        1 cgacgatccc gcggcacgac tggagatcgg actggcgctc tcgcctgtac gtgatagcgt
       61 acgcttgtaa tgagcgtcgc cgtcgagccc cagcacgccg acctgtcggt cggaccccgc
      121 gtccgcgccc tgcgcgacgc catgggcctg tcgctgcgtg agctcgccga ccgcaccggc
      181 gtgtccgcgc cgatgctgtc ccaggtcgag cgcggtgaga ccagcccgac gctggtggtg
      241 gccacccgca tcgccgcggg gctggagctg acgctgtccc agctgctgcg cctggacgag
      301 ggcgggtccg tcagcgtcgt ccgcgccgcc gaccggctca agggcggcac cggccgccgc
      361 ggccacacct acgaggtggt cacgccgccg ctgcccggcc agcgcgccga ggtggccttc
      421 cacaccctgg ccccgggcgc ctcgaccggc ggtcccggcg acccgcccct gcacgagccc
      481 ggcagccgcg agaccgccgt ggtgaccgag ggcgcgctgc gcctgctctg cgacgggcag
      541 gcccacgacc ttcaggaggg cgacgccgtg accttcgacg ccgacctggc ccatcacttc
      601 gagaacccca acgacgagga cgcccgcttc ctggcggtcg tcgcagcagg actcaggagg
      661 agctgaatgc cacagacgtt gttcgacaag atctgggact cccatgaagt agccgacgcg
      721 ctcctctaca tcgacctgca cctggtccac gaggtcacca gcccgcaggc cttcgagtcg
      781 ctgcgcctcc agggccgcaa ggtccgccgc ccggaccgca ccctggccac ggccgaccac
      841 aacgtgccca ccgacggcgc caccaccgcc gagcagatca aggacgtgct ctcgcgcaag
      901 caggtcgagg cgctggagag caactgcaag gagttcggcg tgccggtgca ctcgatcggc
      961 tcgaagctgc agggcatcgt ccacgtgatc gggccggagc tgggcgtgac ccagccgggc
     1021 atgaccatcg tctgcggcga ctcgcacacc gccacccacg gcgccttcgg ggcgctggcg
     1081 ttcggcatcg gcaccagcga ggtcgagcac gtgctggcca cgcagacgct ggcccagagc
     1141 aagccgaagt ccatgcgcat caccttcgag ggcgacgaga tgcccgcagg cgtgaccgcc
     1201 aaggacctga tcctggccac gatcggccag atgggcgtgg ccggcggcac cggccacgtg
     1261 atcgaattcg ccggtccggt catcgagcag ctgtcgatgg aggggcggat gaccatctgc
     1321 aacatgacca tcgagggcgg cgggcgcgcc gggatgatcg cgcccgacga gacgaccttc
     1381 gagtacctga agggccgccc gggcgcgcca cagggcgccg actgggacgc ggccgtcgag
     1441 cactggaaga cgctgcacac cgacgagggc gccacctacg acaaggaggt cgtggtcgac
     1501 gtcgcgtccg tgtcgccgat ggtcacgtgg gggaccacgc cgggccaggt cgtgggcgtc
     1561 tcgggcacgg tgcccgagcc caccagcgac caggagcggc gcgcgctgga gtacatggcg
     1621 ctcgagcccg gcaccccgat gcacgagatc cgtcccgacc gcgtgttcat cggctcgtgc
     1681 accaactcgc ggatcggcga cctgcgcgag gcggcgggcg tgatcgaggg ccgccgggtg
     1741 gccgacggcg tgatcgcgat ggtcgttccg ggcagcgccc aggtgaagga gcaggccgag
     1801 gccgaggggc tggacgaggt gttccgcagc gcgggcttcg actggcgcgg tgccgggtgc
     1861 tcgatgtgcc tgggcatgaa ccccgacgtg gccgcgcccg gcgagcgcgt ggcctcgacc
     1921 tcgaaccgca acttcgaggg gcgccagggc aagggcgcgc gctcgcacct gatgagcccc
     1981 cggatggccg ccgcggccgc catcgagggc catttcgtcg acatccggga ctggagctga
     2041 accgtggagc cgatcaagca gattcaaggc ggtgtctccg tgctgatgcg cgacgacatc
     2101 gacaccgacc agatcatccc cgcgcgctac atgaagcgcg tcgagcgcac cggcttcggc
     2161 gagtacctgt tcgccgacat caaggagaag gccggcttcg acgtgccgcc caacccgatc
     2221 ctggtgaccg gcgtgaactt cggctgcggc tccagccgcg agcatgcccc gtgggcgctg
     2281 gacgactacg gcttcaaggt gatcatcgcc aagtcgttcg ccgacatctt ccgcggcaac
     2341 gcgcccaaga ccggcctgct gccggtggag ctggccgagg acgaggtcga gttcgtcgcc
     2401 gcccaggacg acgcggtggt ggacctggag agccaggagg tgcgcgcggg tgggaggacg
     2461 ttcagcttcg agttccctgc cgagtccaag cgccggctgc tgaacgggct ggacgacatc
     2521 ggcgtgacgc tggactccga ggagaagatc tccgcctacg aggccgagcg cgagcgcaag
     2581 ggccccgaca cgctgacgtt cgcctgagcc gtggccgcga ccgcctggtt cgaccgctgg
     2641 gccacccagt acgacggtga gcggcggctg ctgatctcgc cgttcgacgc gttctacggc
     2701 gcggccgccg aggtggcgtc gctgggacgc acgggggagg gggcccgcgt cctggacctg
     2761 ggggcgggga ccggcctgct gacggccgcc ctggcggacg cccttccggg agcccacttc
     2821 accctcctcg acgaggcccc aggcatgctg gcccaggccc gcgaccgcct ggcgccgctc
     2881 ggggacgatg cggtggcggt ggtgcagggc gacctgatcg ccgacctgcc cgaggggccg
     2941 ttcgacgtga tcgcctccgc tctggccatc caccacctgc cccacgagga ccaggcacgg
     3001 ctgtacgcac tggcccgtga gcggctggcc cccggcggcg tgttcctgaa cgccgagcag
     3061 gtggccgggc cgtcgccggc gctggaggac cacttcgtgg agcgcgagct ggtgcacgcg
     3121 cgcgccggcg ggttcaccgc cgaggcggag gagcaggcct ggatccgctg gtcggcggat
     3181 cgccacgtgc cggtcgagcg ccagatcgag tggctgcacc aggcgggatt cgccaccgtg
     3241 gagtgcgtct acaaggcgtg gcggttcgcg gtcgtcgccg gctggcgctg agcgatgtgt
     3301 agttctcgcg ccgagcgggg ataaggcggc cgtccgggca gggaacgccc ggggctcgtc
     3361 atctactcag cgggagagtt catatgcgaa gcagtactcg ggccgttctg gccacggcac
     3421 tggccatcgg cgcagcggcg ctggctccgg cagcagcgtc tgcctgggct ccggcggaca
     3481 gcgcgaccat ccaccccggc atccagacgg agaccgcggg ctcgggtcag tgcaccgcca
     3541 acttcatctt cacgcgcggc agcgacacgt acatcggaca ggcggcgcac tgcgccagca
     3601 ccggtgcggc caccgacacc gacggctgcc tggcgcagtc cctgccgctg ggcaccaagg
     3661 tgaccctggt gggctcgggc gccaccggca cgctggccta cagctcctgg ctgacgatgc
     3721 agggcaaggg tgagaccgac gccgacgcgt gcgcctacaa cgacctggcg ctggtcaaga
     3781 tcgacccggc cgacgtgtcc aaggtcaacc cgtcggtgcc cggcttcggc ggaccgaccg
     3841 gcctgggcag ccccggcggc gcgctgtcgg acgtctactc gtacggcaat tcgtcgctgc
     3901 gcggcggcat cacccagctc agccccaagc agggcaagct gatccagacc tcaggcaacg
     3961 gctggaacca caccctgtac acgctgaccc cgggcatccc cggtgactcg ggcagcgggt
     4021 tcctgagctc caccggcgcc gccttcggca cgctgagcac cgtggccatc gcgccgctgc
     4081 cggcctccaa cggcgtcggc gacctgtcca aggagctggc ctacgccaac gccaacgggt
     4141 tctccggcac cgcgctggcc aacggcaccg agccgttcac gcccaacatc gtgggcgcga
     4201 tcctcggcgc cggttagcaa cgactccatg c
//
LOCUS       DASUPW010000347         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_636170, whole genome shotgun sequence.
ACCESSION   DASUPW010000347 DASUPW010000000
VERSION     DASUPW010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636170"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="VFY44_07800"
     CDS             <1..1057
                     /locus_tag="VFY44_07800"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5910381.1"
                     /translation="GRRARLDRFFGAIKPGKPTLYLKHVLLPHGPYMFLPSGKQTRRT
                     FRDPLPGMNGPVGFGSRTLTEHNRQRLQLQIAFADREIGRMITRMKANGTYDPSLIAI
                     TADHGISFQVGVKDRRTATRSNIDEVAPVPLFIKAPGQRRGRTSSAYARTVDVVPTIA
                     DLLNFKMPYAADGRSAFSAAVGKRRFVRMIRRGFNGTITVSAKAMEKRRRALVRDKVA
                     KFGQGDFASLYTGIGPNRQLLGKEVAALKPAAQGKVRTQIANAADMRNVDPRSEIHAT
                     QVAGSLTGGKGRGRDLAVAVNGRIEAVGQTFRLRGSSKESYALMVPESALKPGRNTVE
                     LFEVTDKGAKLQLIGKA"
     gene            complement(1067..1705)
                     /locus_tag="VFY44_07805"
     CDS             complement(1067..1705)
                     /locus_tag="VFY44_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910382.1"
                     /translation="MRMPQEERFWRNRLEWRLRGGLQWPAFVVFTLLDAVVLWRLPPL
                     AFQDLNVFDGVFMAVFGNLILLGAVAPFLAKRLIKRRALATPGVPPAQVEREVLQDRV
                     AAILLAVGFAGCVISGLGNRPVIVSETNETQEAARQLELFVRRSGSGELRRNVEAANT
                     HRLEPGYFRLCIPRDDRKRNVCILVDTRKDPVSVRRDPSSSSNQELFGLDDR"
     gene            1728..>2264
                     /gene="lipB"
                     /locus_tag="VFY44_07810"
     CDS             1728..>2264
                     /gene="lipB"
                     /locus_tag="VFY44_07810"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX5910383.1"
                     /translation="MSRTATDELWVARLGRVPYAEAVAIQERLRAARQAGELPDMLLL
                     LEHPPVYTLGRRGDETDLPMGEDWYRSQGIDVERSDRGGRLTYHGPGQLVGYPIMRVA
                     DVPAYVHGLEAAMIAALDDEGVPARVREGLTGVWAGQGKIGAIGIHVSRGVTTHGFAV
                     NVDNDLQPFQWAVACGGEG"
BASE COUNT          354 a          824 c          806 g          280 t
ORIGIN      
        1 cggccgccgc gcgcgcctgg accggttctt cggggcgatc aagcccggca agccgacgtt
       61 gtacctcaag cacgtgctgc tgccccacgg gccgtacatg ttcctgccct cgggcaagca
      121 gacccgccgg accttccgcg acccgctccc gggcatgaac gggccggtcg ggttcggcag
      181 ccggacgctg accgagcaca accgccagcg cctccagctg cagatcgcct tcgccgaccg
      241 cgagatcggg cgcatgatca cccgcatgaa ggcgaacggc acctacgacc cgtcgctgat
      301 cgcgatcacc gccgaccacg ggatctcgtt ccaggtgggg gtcaaggacc gccgcaccgc
      361 cacccgctcg aacatcgacg aggtcgcgcc cgtcccgctg ttcatcaagg cgcccggcca
      421 gcgccgcggc cgcaccagct cggcctacgc gcgcaccgtc gacgtggtgc ccaccatcgc
      481 ggacctcctg aacttcaaga tgccctatgc ggccgacggg cggtcggcgt tctcggccgc
      541 cgtcggcaag cgccgcttcg tgcgcatgat ccggcgcggc ttcaacggca cgatcaccgt
      601 ctcggcgaag gccatggaga agcgccgccg tgcgctggtg cgcgacaagg tcgccaagtt
      661 cggccagggc gacttcgcca gcctgtacac cgggatcggc cccaaccgcc agctcctggg
      721 caaggaggtc gccgcgctca agcccgccgc ccagggcaag gtgcggacgc agatcgccaa
      781 cgccgccgac atgcgcaacg tcgacccgcg ctcggagatc cacgccaccc aggtggccgg
      841 gtcgctgacg ggcgggaagg ggcgcggccg tgacctggcc gtggccgtga acgggcggat
      901 cgaggcggtg ggccagacct tccgcctgcg cgggtccagc aaggagagct acgccctgat
      961 ggtgcccgag tccgcgctca agccggggcg caacacggtc gagctgttcg aggtcaccga
     1021 caagggcgcc aagctccagc tcatcggcaa ggcctagcgc gcggccctag cggtcgtcca
     1081 gcccgaacag ctcctggttc gacgagctgc tggggtcccg gcgcacggag accgggtcct
     1141 tgcgcgtgtc gaccaggatg cagacgttgc gcttgcggtc gtcgcggggg atgcagaggc
     1201 ggaagtagcc gggctcgagc cggtgcgtgt tggcggcctc gacgttgcgg cgcagctcgc
     1261 cgctgcccga ccggcggacg aacagctcca gctggcgggc ggcctcctgg gtctcgttgg
     1321 tctcggagac gatcaccggg cggttgccca ggcccgagat cacacagccc gcgaacccca
     1381 cggccaggag gatggccgcg acccggtcct gcagcacctc gcgctcgacc tgcgccggcg
     1441 ggaccccggg cgtggccagc gcgcgccgct tgatcaggcg cttggccagg aacggcgcca
     1501 ccgcgcccag caggatcagg ttgccgaaca cggccatgaa cacgccgtcg aacacgttca
     1561 ggtcctggaa ggccagcggc ggcaggcgcc acagcaccac cgcgtcgagc agcgtgaaca
     1621 ccacgaaggc gggccactgg aggccgccgc gcaggcgcca ctccaagcgg ttgcgccaga
     1681 aacgctcctc ctgcggcatt cgcacgcaga gtagcctcat acagaccatg tcccgcaccg
     1741 ccaccgacga gctctgggtc gcccggctgg ggcgggtgcc ctacgccgaa gcggtcgcga
     1801 tccaggagcg gctgcgggcg gcgcgccagg ccggcgagct gcccgacatg ctgctgctgc
     1861 tcgagcaccc gcccgtgtac acgctgggcc ggcgaggcga cgagaccgac cttccgatgg
     1921 gcgaggactg gtaccgcagc cagggcatcg acgtggagcg cagcgaccgc ggcggccggc
     1981 tgacctacca cggccccggc cagctggtgg gctacccgat catgcgggtg gcggacgtgc
     2041 cggcgtacgt gcacggcctc gaggcggcca tgatcgccgc gctggacgac gagggcgtgc
     2101 ccgcgcgcgt gcgcgagggc ctgaccgggg tgtgggccgg ccagggcaag atcggcgcga
     2161 tcggcatcca cgtgtcgcgc ggtgtgacca cgcacggctt cgccgtgaac gtggacaacg
     2221 acctgcagcc gttccagtgg gccgtggcct gcggcggcga agga
//
LOCUS       DASUPW010000348         3918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_360649, whole genome shotgun sequence.
ACCESSION   DASUPW010000348 DASUPW010000000
VERSION     DASUPW010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360649"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VFY44_07815"
     CDS             complement(<1..630)
                     /locus_tag="VFY44_07815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5910384.1"
                     /translation="MATVAQDTLLFNPATFDASEFDPETRRVLLATIEWFESRGKQAL
                     KDADHERTWYADFLDFVAREKVFATLLTPAAEAAGDPDKRWDTARIAAFNEITAFYGL
                     VYWYTWQVTILGLGPIWQSTNAAARERAAGLLDEGAIFAFGLSEREHGADIYSTDMIL
                     TPVEGGGFTASGGKYYIGNGNEAGMVSVFGRRSDVEGPEGYVFFAADSGH"
     gene            700..1488
                     /locus_tag="VFY44_07820"
     CDS             700..1488
                     /locus_tag="VFY44_07820"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5910385.1"
                     /translation="MGRSPVPSGDPADVDFFQLMERHLPRAFISVMRRLPSEPRCRLC
                     QAPFGGPGGRVMARLGFGPSRKNPTLCNTCFEKAPMGGVEMEIGVLFADVRGFTSLAE
                     DMAPEGVAKLLNRFYAAASHVLSRSAIIDKLVGDEVMALYVPQLFRGEGWQDDIVRDA
                     RDLLAAVGYGAGGPWLEVGVGVDIGPAFVGNVGAGDVKDFTALGDVVNTAARLQAQAA
                     PGQIVISERLFGRFTAAPGDATPAELSLKGKRAPERARVIDLRA"
     gene            complement(1498..2898)
                     /gene="sufB"
                     /locus_tag="VFY44_07825"
     CDS             complement(1498..2898)
                     /gene="sufB"
                     /locus_tag="VFY44_07825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX5910386.1"
                     /translation="MATPDKTRIPKQSPITEDEQGQLVAQKGISREVIQELSRIKDEP
                     QWMRDLRLKSFEIFERKPVPTWGVDLSDLDLNELVLYSPPEAGRFDSWDDVPEDIKKT
                     YEDLGIPEAEREHLAGVVGTWRQEPVYENLKKEYADLGILFCSMDTAITEYPELVQEY
                     FMKKCVPPQDNKFSALHGAVWSGGSFLYVPKGVKVDLPLQAYFRMEGSGEGTFEHTLI
                     IADEGSEVNYIEGCTAQTYSANSMHSAVVEIFVKEGAKARYTTVQNWSKDVFNLNTKR
                     AIVEAEGTVEWVGGSMGAKKVMLYPGSFLMGEHARADHLNVGVASGDVWKDTGAKVVH
                     LAPNTTSNILAKSISKDGGIMGYRGLVRMGHNSQGSKSHVQCDGLMMDPYSRSDTWPD
                     IQIQNPHTTVAHEAVVGKISDEQLFYLQSRGLSEEEAASMIVNGFIEPVTKELPMEYA
                     IELNRLIQLEMVGSVG"
     gene            3014..>3918
                     /locus_tag="VFY44_07830"
     CDS             3014..>3918
                     /locus_tag="VFY44_07830"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5910387.1"
                     /translation="MSRRSLILVLALAGLVVAGGLVGVVLATSGGDDKLTIYTARSHY
                     GEEKPFKTFAGDQDVNLTLFGGSASELYERLKSEGGNTKADALITVDAANLYRAQAAG
                     LLAENRDPEVEKHVPANLRDPKGRWYGLTVRARTIMRSTERVKPNDVTTYAGLGDPRW
                     KGKLCLRSGTSEYNISFVADRIAKDGAARTKAMLQRWMDNEPTILGSDTDVLKSIANG
                     ACDVGLTNSYYLGRELEEDEDFPVAPVWADQKGRGTHLNLSGIGVVEGADQAEEAKKL
                     VRYLTEPSEQRVFAQNNHEFGVDSLS"
BASE COUNT          592 a         1292 c         1385 g          649 t
ORIGIN      
        1 gtgaccgctg tcggcggcga agaacacgta gccctcgggg ccctcgacgt cggagcgacg
       61 gccgaacacg gacaccatgc cggcctcgtt gccgttgccg atgtagtact tgccgcccga
      121 cgcggtgaag ccgccgccct ccacgggggt gaggatcatg tcggtggagt agatgtcggc
      181 gccgtgctcg cgctcggaca ggccgaaggc gaagatcgcc ccctcgtcca gcaggccggc
      241 ggcgcgctcg cgggcggcgg cgttggtgct ctgccagatc gggccgaggc ccaggatcgt
      301 gacctgccac gtgtaccagt agacgagccc gtagaaggcg gtgatctcgt tgaaggcggc
      361 gatccgggcg gtgtcccagc gcttgtcggg gtcgcccgcg gcctcggcgg ccggggtcag
      421 cagcgtggcg aacaccttct cgcgcgcaac gaagtccagg aagtcggcgt accacgtgcg
      481 ctcgtggtcg gcgtccttga gcgcctgctt gccgcgcgac tcgaaccact cgatcgtggc
      541 caggagcacc cggcgggtct cggggtcgaa ctccgaggcg tcgaaggtgg cggggttgaa
      601 caacagcgtg tcctgcgcga cggtggccat gtccccatgc tctcacgcgg gcgggtgtcc
      661 cgaacccagg atgcttggcc atccggttag agtcgtgcca tgggccggtc accggtgccc
      721 agcggcgatc ccgccgacgt cgacttcttc cagttgatgg agcgacatct gccccgggcg
      781 ttcatctcgg tgatgcgcag gctgccctcc gagccgcgct gccgcctgtg ccaggcgccg
      841 ttcggcggcc cgggcgggcg ggtgatggcc cgcctcgggt tcggcccgtc gcggaagaac
      901 ccgacgctgt gcaacacgtg cttcgagaag gccccgatgg gcggcgtcga gatggagatc
      961 ggggtgctgt tcgccgacgt ccgcgggttc acgtccctgg ccgaggacat ggccccggag
     1021 ggagtggcga agctgctcaa ccggttctac gccgcggcca gccacgtgct cagccggtcg
     1081 gcgatcatcg acaagctcgt gggcgacgag gtgatggctc tgtacgtgcc ccagctcttc
     1141 cggggcgagg ggtggcagga cgacatcgtg cgcgacgccc gcgacctcct ggccgcggtg
     1201 ggctacggag ccggcgggcc gtggctggag gtcggcgtgg gcgtggacat cggccccgcc
     1261 ttcgtgggca acgtgggcgc cggggacgtc aaggacttca ccgcgctagg cgacgtggtg
     1321 aacacggcgg cccgcctgca ggcccaggcc gcccccggtc agatcgtgat ctccgagcgc
     1381 ctgttcggcc ggttcaccgc cgctcccggc gacgcgaccc cggccgagct gtccctgaaa
     1441 ggaaaacgcg cgcccgaacg ggcgcgcgtc atcgacctgc gtgcctgagg gccggcgcta
     1501 gccgaccgag ccgaccattt cgagctggat caaccggttg agctcgatgg cgtactccat
     1561 cggcagctcc ttggtgaccg gctcgatgaa cccgttcacg atcatgctgg ccgcctcttc
     1621 ctcgctcagc ccgcgggact ggaggtagaa cagctgctcg tcggagatct tgcccaccac
     1681 ggcctcgtgg gccacggtgg tgtgggggtt ctggatctgg atgtcgggcc aggtgtccga
     1741 gcgcgagtac gggtccatca tcaggccgtc gcactggacg tggctcttgg agccctgcga
     1801 gttgtggccc atgcggacca gcccgcggta gcccatgatg ccgccgtcct tggagatgga
     1861 cttggccagg atgttcgagg tggtgttcgg ggccaggtgc acgaccttgg cgccggtgtc
     1921 cttccacacg tcaccggagg cgacgcccac gttcaggtgg tcggcgcggg cgtgctcgcc
     1981 catcaggaag gagcccgggt agagcatgac cttcttggcg cccatggagc caccgaccca
     2041 ctcgacggtg ccctcggcct cgacgatggc ccgcttcgtg ttcaggttga acacgtcctt
     2101 ggaccagttc tggacggtgg tgtagcgcgc cttggcgccc tccttgacga agatctccac
     2161 cacggcggag tgcatcgagt tggcgctgta ggtctgcgcg gtgcagccct cgatgtagtt
     2221 cacctcggag ccctcgtcgg cgatgatgag ggtgtgctcg aaggtgccct cgcctgagcc
     2281 ctccatgcgg aagtaggcct gcagcggcag gtcgaccttg acgcccttgg gcacgtagag
     2341 gaacgagccg ccggaccaga ccgcgccgtg cagtgccgag aacttgttgt cctgcggcgg
     2401 gacgcacttc ttcatgaagt actcctgcac cagctcgggg tactccgtga tggcggtgtc
     2461 catcgagcag aagaggatgc ccaggtcggc gtactccttc ttcaggttct cgtacaccgg
     2521 ctcctggcgc caggtgccca cgacgccggc caggtgctcg cgctcggcct cggggatgcc
     2581 caggtcctcg taggtcttct tgatgtcctc cgggacgtcg tcccaggagt cgaaccggcc
     2641 ggcctcgggc gggctgtaga gcaccagctc gttcaggtcc aggtcggaca ggtccacgcc
     2701 ccaggtcggg acgggcttgc gctcgaagat ctcgaacgac ttgagccgga ggtcgcgcat
     2761 ccactggggc tcgtccttga tccgggagag ctcttggatg acctcgcgcg agatcccctt
     2821 ctgggccacc agttggccct gctcgtcctc ggtgatgggg ctctgcttgg gaatgcgcgt
     2881 cttgtccgga gtagccatga agtccttgtg cgtcggggta cgaaatccgc tcggctgagt
     2941 cggatatccg attgtaccta cgtggcgccc gaacgaccgg atcgaaagcg ggtttaggct
     3001 agcctaagcg gtcgtgtcca gacgctccct gatcctggtc ctggcgctcg ccgggctggt
     3061 cgtggccggt ggtctggtgg gcgtcgtgct cgccacctcc ggcggtgacg acaagctcac
     3121 catctacacg gcgcgctcgc actacggcga ggagaagccg ttcaagacct tcgccggcga
     3181 tcaggacgtg aacctgaccc tgttcggcgg ttcggcctcc gagctctacg agcggctcaa
     3241 gagcgagggc ggcaacacca aggccgacgc gctgatcacc gtggacgccg ccaacctcta
     3301 ccgggcacag gcggccggcc tgctggccga gaaccgcgac cccgaggtcg agaagcacgt
     3361 gcccgccaac ctccgcgacc ccaagggccg ctggtacggg ctgacggtgc gtgcgcggac
     3421 gatcatgcgc tccaccgagc gggtcaagcc gaacgacgtg accacgtacg ccggcctggg
     3481 cgacccgcgc tggaagggca agctgtgcct gcgctcgggc accagcgagt acaacatctc
     3541 gttcgtcgcc gaccggatcg ccaaggacgg cgcggcccgg acgaaggcga tgctccagcg
     3601 ctggatggac aacgagccga cgatcctggg ctcggacacc gacgtgctga agtcgatcgc
     3661 caacggcgcc tgcgacgtgg gcctgaccaa ctcgtactac ctgggccgcg agctggagga
     3721 ggacgaggac ttcccggtcg cgcccgtgtg ggccgaccag aaggggcgcg gcacgcacct
     3781 gaacctgtcg gggatcggcg tggtcgaggg ggccgaccag gccgaggagg cgaagaagct
     3841 cgtgcgctac ctgaccgagc cctccgagca gcgcgtgttc gcgcagaaca accacgagtt
     3901 cggcgtcgac tcgctgtc
//
LOCUS       DASUPW010000349         2289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1053057, whole genome shotgun sequence.
ACCESSION   DASUPW010000349 DASUPW010000000
VERSION     DASUPW010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053057"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..1030
                     /locus_tag="VFY44_07835"
     CDS             125..1030
                     /locus_tag="VFY44_07835"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEX5910388.1"
                     /translation="MRLLTGVLVLQIALAATLTVLVATDNVPFVSGQDVRSGNEAQAA
                     VPRATSKHFDSRAAYNSVKRQVALGPRPAGSAASKVLAERLRRALPGGRFQAVPGGLR
                     NVIGVVPGKTRRVVVVGAHYDTKDLPGFVGANDGAAGTAVMVQLARTIKPRTLKPTLV
                     FIAFDGEEAPAGVPDADFAAEGLRGSKVAAKRYKNASAMVLLDFIGQRNLRLLADASA
                     DADLWGKVRRAGRRAGVGRIFPSDVQGSVQDDHTPFLRQGVPAVDLIDFDYACFHRTC
                     DNLAQIHEPSLDATGETMMKLLPTL"
     gene            1036..1989
                     /locus_tag="VFY44_07840"
     CDS             1036..1989
                     /locus_tag="VFY44_07840"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF002189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX5910389.1"
                     /translation="MPTAPEKLLLAAPRGYCAGVDRAVQTVEHALDLYGAPVYVRKEI
                     VHNKHVVEQLRERGAIFVEQESEVPEGETVVFSAHGVSPAVHANAEARELKTIDATCP
                     LVTKVHVEAKKFAAEGYTIVMIGHNGHEEVEGTMGEAPDHIILIETEDEVDDLEVPDP
                     DKVAFISQTTLAVDETRAIILRLREKFPNITGPRTDDICYATTNRQMAVRQMAVECDL
                     VLVIGSRNSSNSNRLVEVARDHGADSYLIDNETQVREEWLEGKRVVGISSGASAPEEL
                     VQRLVDFFRERGTDDVSEFEVVQEDVRFMLPKQIRKALAAQ"
BASE COUNT          362 a          825 c          780 g          322 t
ORIGIN      
        1 cccacgtgca tgtgcgagtt ctcccgctcc tcgtgcagga aggaggcgtc gaccacgctc
       61 aggcggtcct tgtggccctg cgccatggcg ggagactaac ccggcgtccg ccacaatggg
      121 tgcgatgcgc ctgctcaccg gagtcctcgt cctccagatc gccctggccg cgacgctcac
      181 cgtcctcgtg gccaccgaca acgtgccgtt cgtctccggc caggacgtgc gcagcggcaa
      241 cgaggcccag gccgcggtgc ccagggccac ctcgaagcac ttcgactccc gggccgccta
      301 caactcggtc aagcgccagg tggcgctggg accgcggccc gcgggctcgg cggcctcgaa
      361 ggtgctggcc gagcgcctgc gccgcgcgct gcccggcggc cgcttccagg cggtgcccgg
      421 cggcctgcgc aacgtcatcg gcgtggtgcc cggcaagacg aggcgcgtgg tcgtggtcgg
      481 cgcgcactac gacaccaagg acctgcccgg gttcgtgggc gccaacgacg gggcggccgg
      541 caccgcggtg atggtccaac tggcgcgcac gatcaagccc cggacgctga agccgacgct
      601 ggtgttcatc gccttcgacg gcgaggaggc ccccgccggg gtcccggacg cggacttcgc
      661 cgccgagggc ctgcgcggca gcaaggtggc cgccaagcgg tacaagaacg cctcggcgat
      721 ggtgctgctg gacttcatcg gccagcgcaa cctgcgcctg ctggccgacg ccagcgccga
      781 cgccgacctg tggggcaagg tgcgcagggc cggccgcagg gcgggcgtgg gcaggatctt
      841 cccgtcggac gtccagggca gcgtccagga cgatcacacg ccgttcctca ggcagggcgt
      901 gccggcggtg gacctgatcg acttcgacta cgcctgcttc caccgcacct gcgacaacct
      961 ggcccagatc cacgagccca gcctggacgc gaccggtgag acgatgatga agctcctgcc
     1021 tacactctga ggctcatgcc tacggcaccg gaaaagcttc tcctggccgc cccgcgcggc
     1081 tactgcgcgg gtgtggaccg ggcggtccag accgtcgagc acgccctcga cctctacggc
     1141 gcccccgtgt acgtgcgcaa ggagatcgtg cacaacaagc acgtggtcga gcagctgcgc
     1201 gagcgcggcg ccatcttcgt cgagcaggag agcgaggtgc ccgagggcga gaccgtggtg
     1261 ttctcggccc acggtgtctc accggcggtg cacgccaacg ccgaggcgcg cgagctgaag
     1321 accatcgacg ccacctgccc gctggtgacc aaggtgcacg tggaggccaa gaagttcgcg
     1381 gccgagggct acacgatcgt gatgatcggc cacaacggcc acgaggaggt cgagggcacg
     1441 atgggcgagg cgcccgacca catcatcctc atcgagaccg aggacgaggt ggacgacctc
     1501 gaggtgcccg accccgacaa ggtcgccttc atctcccaga ccacgctcgc ggtggacgag
     1561 acccgcgcga tcatcctcag gctgcgcgag aagttcccca acatcacggg gccgcgcacc
     1621 gacgacatct gttacgccac cacgaaccgc cagatggccg tccgccagat ggccgtcgag
     1681 tgcgacctgg tgctggtcat cggctcacgc aactcgtcga actccaaccg gctggtcgag
     1741 gtcgcccgcg accacggcgc cgactcctac ctgatcgaca acgagaccca ggtgcgcgag
     1801 gagtggctgg agggcaagcg cgtggtgggc atctcctccg gcgccagcgc ccccgaggag
     1861 ctggtccagc gcctggtcga cttcttccgc gagcgcggca ccgacgacgt gtcggagttc
     1921 gaggtcgtcc aggaggacgt gcgcttcatg ctccccaagc agatccgcaa agccctagcg
     1981 gcgcaataag cgtcgcctgg ctgcgtcctc ggcctgctcg tgtgcgctcg cacacgtcgc
     2041 agacctcttc cttgccatgc tcgcttcttg caccgctatg tctttgcggg cggaaggcgc
     2101 gttgctgggg ccaacgcgag tgccgaggac gccgggatgc gatgcctcag ggcgccttgg
     2161 gcgtgaactg gaagccgagc aggcgtagcc ctgcggcttc cgccgtgatc gtcccgtccg
     2221 tgcggtgcag ccccggctcc accgcgcccg acagcaccgc gtacgcctcg ccgccctgcc
     2281 acggcaccg
//
LOCUS       DASUPW010000350         2549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_499552, whole genome shotgun sequence.
ACCESSION   DASUPW010000350 DASUPW010000000
VERSION     DASUPW010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499552"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..522)
                     /locus_tag="VFY44_07845"
     CDS             complement(52..522)
                     /locus_tag="VFY44_07845"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX5910390.1"
                     /translation="MEAARIARFTNRSARNRRASVPFARAHAWLYRASGGRLAPRWFG
                     APVLVLETVGRRSGQPRATPVLFVLQEGDPVVLAANAGARRAPAWWLNLRDAGRGVAV
                     MGRERREVTPRVTAGAERAELWDRLCSAYPAAAHYPEFTDRELPVVVLERLRGR"
     gene            complement(525..983)
                     /locus_tag="VFY44_07850"
     CDS             complement(525..983)
                     /locus_tag="VFY44_07850"
                     /inference="COORDINATES: protein motif:HMM:NF023010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HEX5910391.1"
                     /translation="MPDSVELRHLFDMHVDLEPAQVIASPEVTRMVFVARGGTVRGDR
                     IEAEVLPGGGEWLRVGTDGVGRIDVRATMRTHDGELVLLTNTGVIAIAGEARERFGAG
                     QSLEWNELHARSSPQFETGAEQYAWLNSVVTVAVHGLGPRHVDYRVFEAL"
     gene            complement(989..1378)
                     /locus_tag="VFY44_07855"
     CDS             complement(989..1378)
                     /locus_tag="VFY44_07855"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5910392.1"
                     /translation="MTTATTLDLEHVTDVYFAAWEALDPDRIAALHSADTQFQIHTAG
                     SEPVIGRDHVREAFANVFAQFPGYAFQTHRLLIGDDHWVLDWTLLSDIGELGCLDVVT
                     VDADGLVRRKDTYIEPADLEAVFGSAA"
     gene            complement(1375..2184)
                     /locus_tag="VFY44_07860"
     CDS             complement(1375..2184)
                     /locus_tag="VFY44_07860"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5910393.1"
                     /translation="MIVRRLGWAGIELEAEGATLIVDLMEDASGMEPFVGPPRGPLPS
                     PSRTGALAGLVTHLHADHCDAAALSRALAPGAPVLRPAPASGAGLETAALEEAEQGLR
                     RHALATRVVAPWEDLTVGPFTVTALPAVDGFGDPQVSWAVEAGGHRVLHAGDTLFHGW
                     WWLARMRRGPFDAVFLPVNGAVVDLPHRQPPSPLSADMDPGQAAVAASILAPGVAVPI
                     HYDTLHRPPTYAQVDDPAGHFLTACAAAGVEARVLETGDSLDLTRLPERTA"
     gene            2232..>2549
                     /locus_tag="VFY44_07865"
     CDS             2232..>2549
                     /locus_tag="VFY44_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5910394.1"
                     /translation="MSPRKSAAEAQETRAAIVEHAIDVASLEGLEGLTIGRLAGDMRM
                     SKAGVVGGFGSKEGLQLATLEGAIAVWRREVWERVEDVEPGLPRLRAVCEAWVTYLDG
                     DVFP"
BASE COUNT          346 a          931 c          959 g          313 t
ORIGIN      
        1 gcgagcaccg agaccaccac cggaacgggg acccgcggcc tggcggccga gtcaacggcc
       61 ccgcagccgc tccagcacca ccaccggcag ctcccggtcg gtgaactccg ggtagtgggc
      121 cgccgccggg tacgccgagc acaggcggtc ccacagctcg gcccgctcgg cgcccgccgt
      181 cacccgcggc gtgacctcgc gccgctcgcg gcccatcacg gcgacgccgc gcccggcgtc
      241 gcgcaggttc agccaccacg ccggcgcgcg ccgggcaccg gcgttggcgg ccagcaccac
      301 ggggtcgccc tcctgcagga cgaacagcac cggcgtggcg cggggctgcc cgctgcgccg
      361 gcccaccgtc tccagcacca gcacgggcgc gccgaaccag cgcggcgcga ggcgcccgcc
      421 gctggcgcgg tacagccagg cgtgggcgcg cgcgaacggg acgctggccc tgcggttgcg
      481 cgcgctgcgg ttggtgaagc gggcgatccg ggcggcctcc acggtcagag cgcctcgaac
      541 acgcggtagt ccacgtggcg gggaccgagg ccgtggacgg ccacggtgac caccgagttc
      601 agccacgcgt actgctcggc cccggtctcg aactggggcg acgatcgcgc gtgcagctca
      661 ttccactcca ggctctggcc cgcgccgaag cgctcccgcg cctcacccgc aatggcgatc
      721 acaccggtgt tggtgagcag caccagctct ccgtcgtggg tccgcatggt ggcgcggacg
      781 tcgatccggc ccacgccgtc ggtgcccacc cgcagccatt cgcctccgcc gggcagcacc
      841 tcggcctcga tccggtcgcc ccgcaccgtg ccgcccctgg ccacgaacac catccgggtg
      901 acctcgggcg aggcgatcac ctgcgcgggc tcgaggtcca cgtgcatgtc gaacaggtgg
      961 cgcagctcga cggagtccgg catgcgcatc aggcggcgct cccgaacacg gcctcgaggt
     1021 cggccggctc gatgtaggtg tccttgcgcc ggaccaggcc gtcggcgtcc acggtgacca
     1081 cgtccaggca gcccagctcg ccgatgtcgg agagcagggt ccagtccagc acccagtggt
     1141 cgtcgccgat cagcagccgg tgggtctgga acgcgtagcc ggggaactgg gcgaacacgt
     1201 tggcgaaggc ctcccggacg tggtcgcgcc cgatcaccgg ctccgagccc gcggtgtgga
     1261 tctggaactg cgtgtcggcg ctgtgcaggg cggcgatccg gtcggggtcg agcgcctccc
     1321 aggcggcgaa gtacacgtcg gtcacgtgct cgaggtcgag ggtggtggcg gtggtcatgc
     1381 ggtcctctcg ggtagtcggg tcagatccag cgagtcgccg gtctcgagca cacgggcctc
     1441 gactccggcg gcggcacagg cggtcaggaa gtggccggcg gggtcgtcca cctgggcgta
     1501 ggtgggcggg cggtggagcg tgtcgtagtg gatcggcacg gcgacgccgg gcgccaggat
     1561 cgaggcggcg acggccgcct gcccggggtc catgtccgcg ctgagcggcg acggcggctg
     1621 acggtgcggc aggtccacca cggcgccgtt caccggcagg aacacggcgt cgaacgggcc
     1681 gcggcgcatg cgcgccagcc accaccagcc gtggaagagc gtgtcgcccg catgcaggac
     1741 ccggtgcccg ccggcctcga ccgcccacga cacctggggg tcgccgaagc cgtcgaccgc
     1801 ggggagcgcg gtcaccgtga acgggccgac ggtgaggtcc tcccacggag ccaccacccg
     1861 ggtcgccagc gcgtggcgcc tgagcccctg ctcggcctcc tccagggcgg cagtctcgag
     1921 gccggcgccg cttgcgggcg cgggccgcag gaccggtgcg cccggcgcca gcgcccggga
     1981 cagcgcggcc gcgtcgcagt ggtccgcatg caggtgcgtg accaggcccg ccagggcgcc
     2041 ggtccgcgac ggcgacggca acggcccgcg gggcgggccc acgaacggct ccatcccgct
     2101 ggcgtcctcc atcaggtcga cgatcagggt cgccccctcc gcctccagct cgattccggc
     2161 ccagccgagt cgtctcacga tcatgcgatc cagttttagc agacgatcgt tcgtttggta
     2221 aagtcggatg tatgagcccc cggaaaagcg cagccgaggc ccaggagacg cgggccgcga
     2281 tcgtggagca cgccatcgac gtggcctcgc tggagggcct ggaaggcctg acgatcgggc
     2341 ggctggccgg cgacatgcgc atgagcaagg cgggggtggt cggcggcttc ggctcgaagg
     2401 aggggctgca gctggccacc ctcgaggggg cgatcgccgt gtggcggcgc gaggtctggg
     2461 aacgggtcga ggacgtcgag cccggcctcc cccgcctgcg cgccgtctgc gaggcgtggg
     2521 tgacctacct ggacggcgac gtgttcccg
//
LOCUS       DASUPW010000351         8127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1745805, whole genome shotgun sequence.
ACCESSION   DASUPW010000351 DASUPW010000000
VERSION     DASUPW010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8127
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1745805"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VFY44_07870"
     CDS             <1..571
                     /locus_tag="VFY44_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910395.1"
                     /translation="TNTWTDELMAELAATGAVDSVDLKGHYKGTPVDVVTDAEMYRRV
                     ARAFPDAWIEDPDLSDPDAAAALEGEHDRITWDAPIHSVADIEALAFPPKTVNVKPSR
                     LGGLRSLCAAYDYCEQHGIGAYGGGQTELGVGRDHIQYLAATFHPDTPNDTAPRGFNQ
                     NPVPDGLPTSPLQVPVADTGFRFREDAGT"
     gene            568..951
                     /gene="mscL"
                     /locus_tag="VFY44_07875"
     CDS             568..951
                     /gene="mscL"
                     /locus_tag="VFY44_07875"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX5910396.1"
                     /translation="MIKEFRDFILRGNLVDLAVAVVIGTAFAVLVKSLTDNLITPVLA
                     MIGGKPDFSDLSFTINNAEFRYGAFLTDVIGFLIVAAVIFFLVVKPVNVLMERARAGD
                     TEDEAPDPQIVVLEEIRDLLKAERR"
     gene            962..1498
                     /locus_tag="VFY44_07880"
     CDS             962..1498
                     /locus_tag="VFY44_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HEX5910397.1"
                     /translation="MPAEQFVTALNDQIGREFGASQQYAAVAVYYDDLTLPQMAAFFY
                     RQSLEERDHAMMMVQYLLDADETPVIPGVDAPQVEFSDIVEPVKVALEQEKRVTDQIS
                     SLASVARDANDFVSEQFTQWFLKEQIEEVATMSDLVAVCERSKDRPSEIEDYLVREHP
                     GDEGEDPTAPQAAGGPVA"
     gene            complement(1495..1926)
                     /locus_tag="VFY44_07885"
     CDS             complement(1495..1926)
                     /locus_tag="VFY44_07885"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5910398.1"
                     /translation="MDQRVSVVTLGVGDVARSRAFYTGLGWEPTLEPDGRVIFFQAGG
                     LVFALWALEDMEAETGVSGGRPGSAMLAHNVGTDAEVDELLAAAAQAGGDVVAPARRM
                     EWGGYSGSFADPDGHLWEVAHNPFWTIYEDGTVSLQPPGTP"
     gene            complement(1930..2751)
                     /locus_tag="VFY44_07890"
     CDS             complement(1930..2751)
                     /locus_tag="VFY44_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910399.1"
                     /translation="MPYSRTIAAVACIAGLLAVPAATTAAKPKSAKSHVVKTPKGNQL
                     AVPITVRYKLDGKGKSLKRSTLATRVTGAAKLPDGRVLRATETRRTPGKARVRIEHHV
                     FYSVRQTKILRKALAAKQQVKLTVASALQADLDGDGKTDARTTQKTTQVLSAPTAEKS
                     PPAVGHQQGNNPCGVLGLSSSICSNVSVGTWTAGHFWDSKMWNFTCPSAFPYSAGSVS
                     TQTSSKHYTQTAYTTGNADQTTQITIIDDNVRGHPISYTPTAACTTTDTTSGRIP"
     gene            complement(2802..4259)
                     /locus_tag="VFY44_07895"
     CDS             complement(2802..4259)
                     /locus_tag="VFY44_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910400.1"
                     /translation="MRRLPAVLLALAALAALAPAVAQARARFDTRVLAKVPAPGYPAQ
                     AYVHPNGRVYEGTYINPNAGAMPSRVFEFDGQDGTLLRSWSVPGQNLSGQQAVQVTTS
                     DANGDLVILDNNPARVLKLDRDTGDFRVYSTFADVEGGAAPRPNYGAWGPDGSLYVTD
                     YGQGLIWRVPPGGGPAVVWLRSAKLEGSVDFGTTGLALAGDRRTLLVAQGTSAIGNGE
                     LNPATGKIYRVPIDPAGRPGPVTKLWESAAADVPDGFAIAASGRLYVPMVGLSAQIAV
                     VAPDGREIERFPRAAGSGENGSPVPFDSPSSARFLGTRLIVANQSALAGTAANQALLD
                     VEAGETGLDELIPGRDATAPRLTKVSVAPKRFAAIRRGGKPRRARKGRPARGSKLRLT
                     TSERVTVAVRVERRARGRWRRVQSFERDLAAGRRAIRFGGRVSAGKRTRALAAGRYRF
                     SIQAADPAGHRSPRLFREFRLVSGNRPVARKAARP"
     gene            complement(4256..5773)
                     /locus_tag="VFY44_07900"
     CDS             complement(4256..5773)
                     /locus_tag="VFY44_07900"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HEX5910401.1"
                     /translation="MSDAPLSRRRFLGTGVAAGATAAAAALPGVDDAEAAKRKKKRKR
                     GRGTRTVDVAIVGAGFAGLTAARELVRDGRSVCVLEGRDRVGGRVWNRELGKGIESER
                     GGTFVGPTQDHVIKLADTLGIGRFKTFNDGENVYVADGQRSTFSDTLPTGSAPLDPTI
                     LLDLATVIAALDEMAATVPPDAPWRAARAKEWDGQTFESWLNANSTSERFRRLAAVAC
                     RPIFGGEPRELSLLFVLYYIASSGNERTLGTFERNFNTRDGAQEQRFVGGSQQIAHEL
                     HKRLGKRVVLRSPVKKIVQGKKGVRVESSRLIVKAKRVIVAIPPALAGQIDFSPDLPA
                     ERAALNARVPQGTLLKVAAVYDKPFWRAKGLNGQALSLSGPVAATFDDSPPSGSPGVV
                     FGFIGGDDARAFRKQSASARRAAVLENFATFFGPEARAPKDYFETDWPGERFSRGGPV
                     GILGPGTLTAYGPALRKPVRRVHWAGTETAIYWNGYMDGAVSSGERAAREVSDAL"
     gene            complement(5770..6432)
                     /locus_tag="VFY44_07905"
     CDS             complement(5770..6432)
                     /locus_tag="VFY44_07905"
                     /inference="COORDINATES: protein motif:HMM:NF037005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5910402.1"
                     /translation="MTPPASTSDSPTLRREPQQARSRARLAGLLDAAEKLLVREGPEA
                     LTTTRIAEEAGVSVGSLYRWFPDKAAIVDALTRRYLAEFEATIDELAAAAPTAEWDDL
                     AGMLLDVFAHRGVSQPGYRALWLGRQFSEELQAADRANKAILADGVRRILVGRRVIAD
                     TEEAATACRAGVLAADALLHEAFRLDPDGDPGLMDEGKELLRAYLRTAARRFPPKENA
                     AP"
     gene            6510..7175
                     /locus_tag="VFY44_07910"
     CDS             6510..7175
                     /locus_tag="VFY44_07910"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX5910403.1"
                     /translation="MGVREAREQVAAVARRLAAEGLVLGTAGNVSQRDGDRVAVTPTG
                     AVLAELEAADVCVVALDGSPIEGPLAPTSELDLHLGAINRHGCGAVVHTHAPMSTAAA
                     CVLDELPCIHYAMLALGGDVPVAPYTTFGTPELAASVVDALEGRTAALMASHGAVTVG
                     ADAAAAMDLALLLEWAATVYLGAAQAGTPHVLDQAERTAVIEAVVASGYGTTKRVDDG
                     EAL"
     gene            7172..8104
                     /locus_tag="VFY44_07915"
     CDS             7172..8104
                     /locus_tag="VFY44_07915"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX5910404.1"
                     /translation="MRAIAMGVHVADVLARPVEEIPAGQGGALVDEIRITAAGSAGGT
                     ALTLAKLGGAVTSAGAIGTDVLGDVLVRLLEDGGVDPSLLVRRDDVQTSASVLPIRPN
                     GDRPALHVVGANGTYGPDDAPWDAIRDATHLHLGAPEFMGGEAAARILSYAREHGVTT
                     SADILAPGEQAAQIVDWIGPAFAHLDYLLPNDEQVLALSGQDDLERGARVLMERGVGC
                     VAATAGADGAIVVSPEGTERVPAFPVDVVDTTGCGDAFSAGFLRGRSLDRSLRESAVL
                     GCAAAGLVAGGLGSDHGDFDLADCDALAAGASVG"
BASE COUNT         1053 a         2934 c         3009 g         1131 t
ORIGIN      
        1 gaccaacacg tggaccgacg agctgatggc cgagctggcc gccaccggcg ccgtcgactc
       61 ggtcgacctc aagggccact acaagggcac gccggtggac gtggtcaccg acgccgagat
      121 gtaccggcgc gtggcccggg cgttccccga cgcctggatc gaggaccccg acctgtccga
      181 ccccgacgcg gccgccgccc tggagggcga gcacgaccgg atcacgtggg acgcgccgat
      241 ccactcggtg gccgacatcg aggcgctggc cttcccgccc aagaccgtga acgtcaagcc
      301 ttcgcgcctg ggcgggctcc ggtcgctgtg cgcggcgtac gactactgcg agcaacacgg
      361 catcggcgcc tacggcggcg gtcagaccga gctgggggtc ggacgcgacc acatccagta
      421 cctggcggcc accttccacc ccgacacgcc caacgacacg gccccgcggg gcttcaacca
      481 gaacccggtg cccgacgggc tgcccacgag cccgctgcag gtgcccgtgg ccgacaccgg
      541 gttccgattc cgagaggacg ccggcacgtg atcaaggagt tccgggactt catcctgcgg
      601 ggcaacctcg ttgacctggc ggtggcggtg gtcatcggca cggcgttcgc ggtgctggtc
      661 aagtcgctca ccgacaacct gatcacgccg gtgctggcca tgatcggcgg caagccggac
      721 ttcagcgacc tgtcgttcac catcaacaac gccgagttcc ggtacggcgc gttcctcacc
      781 gacgtcatcg gcttcctgat cgtcgccgcg gtcatcttct tcctcgtggt caagcccgtg
      841 aacgtgctca tggagcgcgc ccgcgccggg gacaccgagg acgaggcgcc cgatccgcag
      901 atcgtggtcc tcgaggagat ccgcgatctg ctcaaggccg agcggcgata gggtcagcgc
      961 catgcccgct gaacagttcg tcaccgcctt gaacgaccag atcggccgcg agttcggggc
     1021 ctcccagcag tacgcggcgg tggccgtcta ctacgacgac ctgacgctgc cccagatggc
     1081 ggcgttcttc tatcgccagt cgctggagga gcgcgaccac gcgatgatga tggtccagta
     1141 cctgctggac gccgacgaga cgccggtgat ccccggggtg gacgcgccgc aggtcgagtt
     1201 ctccgacatc gtcgagccgg tcaaggtcgc gctggagcag gagaagcgcg tgaccgacca
     1261 gatctcgtcg ctggcctcgg tggcgcgcga cgccaacgac ttcgtcagcg agcagttcac
     1321 ccagtggttc ctcaaggagc agatcgagga ggtggccacc atgtccgacc tggtggccgt
     1381 gtgcgagcgg tcgaaggacc gcccgtccga gatcgaggac tacctggtcc gcgagcaccc
     1441 gggcgacgag ggcgaggacc ccaccgcgcc tcaggcggcc ggcggcccgg tcgcctaggg
     1501 cgtgccgggc ggctgcagcg agaccgtgcc gtcctcgtag atcgtccaga aggggttgtg
     1561 ggccacctcc cagaggtggc cgtcgggatc ggcgaagctg cccgagtagc cgccccactc
     1621 catccgccgc gcgggggcca cgacgtcgcc cccggcctgc gcggcggcgg ccagcagctc
     1681 gtcgacctcg gcgtcggtcc ccacgttgtg ggccagcatg gccgagccgg gccggccgcc
     1741 gctgacgccg gtctcggcct ccatgtcctc cagcgcccag agggcgaaca ccagcccgcc
     1801 ggcctggaag aagatcacgc ggccgtcggg ctccagcgtc ggctcccagc ccaggccggt
     1861 gtagaaggcg cgggagcggg cgacgtcgcc gacgcccagc gtgacgacgc tgacccgctg
     1921 gtccactagc tagggaatcc tgccgctggt ggtgtccgtc gtcgtgcagg cggcggtcgg
     1981 cgtgtaggag atcggatggc cgcggacgtt gtcgtcgatg atcgtgatct gcgtggtctg
     2041 gtcggcatta ccggtggtgt acgcggtctg cgtgtagtgc ttggagctgg tctgggtgct
     2101 caccgagccg gcgctgtagg ggaacgcgga ggggcaggtg aagttccaca tcttgctgtc
     2161 ccagaaatga ccggcggtcc aggtgccgac ggacacgttg ctgcagatgg aagagctcag
     2221 ccccagcacg ccgcacgggt tgttgccctg ctggtggccg acggcgggcg gggacttctc
     2281 agcggtcggc gcgctcagca cctgggtggt cttctgggtc gtccgggcgt cggtcttgcc
     2341 gtcgccgtcc aggtcggcct ggagcgccga ggccaccgtc agcttgacct gctgcttggc
     2401 ggccagcgcc ttgcgcagga tcttggtctg gcggaccgag tagaagacgt ggtgctcgat
     2461 cctcacgcgg gccttaccgg gcgtgcgccg ggtctcggtg gcacgcagca cgcgcccgtc
     2521 cggcagcttc gcggcgccgg tcacgcgggt cgccagggtc gaccgcttca ggctcttgcc
     2581 cttgccgtcg agcttgtagc gcacggtgat cggcacggcc agctggttgc ccttcggcgt
     2641 cttgaccacg tgcgacttcg ccgacttcgg cttggccgcg gtcgtggccg cgggtaccgc
     2701 gagcagtcct gcgatgcagg cgacggcggc gatggtgcgt gagtacggca acgaattcct
     2761 cctgagtaac tggcccctgc ccggaatcgg acggcccaag cctacgggcg tgcggccttg
     2821 cgtgccaccg ggcggttgcc cgacaccagg cggaactcgc ggaacagccg cggtgagcgg
     2881 tggcccgcag ggtcggcggc ctggatcgag aaccggtagc gcccggccgc cagggcgcgc
     2941 gtgcgcttcc cggcgctgac gcgtcctccg aaccggatgg cgcgccgccc ggcggccagg
     3001 tcgcgctcga aggactggac ccggcgccag cgtccacgcg cccgccgctc gacccggacg
     3061 gccaccgtca cgcgctcgct ggtggtcagg cgcagcttgg acccgcgcgc cgggcggccc
     3121 ttgcgcgcgc gccggggctt gccgccgcgg cggatggcgg cgaaccgctt cggcgccacc
     3181 gacaccttcg tcagcctggg cgcggtggcg tcgcggccgg ggatcagctc gtccaggccc
     3241 gtctcgcccg cctcgacgtc caggagcgcc tggttggcgg cggtgccggc cagcgcgctc
     3301 tggttggcca cgatcagccg ggtgcccagg aagcgggcgc tcgaggggga gtcgaagggc
     3361 acgggcgagc cgttctcgcc gctgcccgcg gcgcgcggga agcgctcgat ctcgcgcccg
     3421 tcgggcgcga ccacggcgat ctgcgccgac agcccgacca tgggcacgta gaggcggccg
     3481 ctggccgcga tggcgaagcc gtccggcacg tcggccgcgg cgctctccca cagcttggtc
     3541 acgggcccgg gccgccccgc ggggtcgatc gggacgcggt agatcttgcc cgtcgccggg
     3601 ttcagctcgc cgttgccgat cgccgaggtg ccctgggcca ccaggagcgt gcgccggtcg
     3661 ccggccaggg ccaggcccgt ggtgccgaag tcgaccgagc cctcgagctt tgcgctgcga
     3721 agccagacca cggcgggacc gccacccggc ggcacgcgcc agatcaggcc ctggccgtag
     3781 tcggtcacgt acaggctgcc gtccggtccc caggcgccgt agttgggccg gggcgcggcc
     3841 ccgccctcca cgtcggcgaa ggtcgagtac acgcggaagt cgccggtgtc gcggtccagc
     3901 ttgagcacgc gcgccgggtt gttgtccagg atcaccaggt cgccgttcgc gtcgctggtg
     3961 gtcacctgca cggcctgctg gccggacagg ttctggccgg gcacgctcca ggagcgcagc
     4021 agggtgccgt cctggccgtc gaactcgaac acgcgcgagg gcatggcgcc cgcgttgggg
     4081 ttgatgtacg tgccctcgta gacgcgcccg ttggggtgca cgtaggcctg ggccggatac
     4141 ccgggcgccg gcaccttggc cagcacgcgc gtgtcgaagc gcgctcgcgc ctgggccacg
     4201 gcgggggcca gcgccgccag cgcggccagg gccaggagga ctgccggcag ccgcctcaca
     4261 gcgcgtcgct gacctcacgg gcggcgcgct cgccggagct gaccgcgccg tccatgtagc
     4321 cgttccagta gatcgccgtc tcggtgccgg cccagtgcac gcgccgcacg ggcttgcgta
     4381 gggcggggcc gtaggcggtc agcgtgcccg ggccgaggat gcccaccggg ccgccgcgcg
     4441 agaagcgctc gcccggccag tcggtctcga agtagtcctt gggcgcgcgt gcctcggggc
     4501 cgaagaaggt ggcgaagttc tccagcaccg cggcccgccg cgccgacgcc gactgcttcc
     4561 ggaaggcgcg cgcgtcgtcg ccgccgatga agccgaagac cacgcccggg gacccgctgg
     4621 gcggcgagtc gtcgaaggtg gcggccacgg ggccgctgag cgacagcgcc tggccgttca
     4681 ggcccttggc gcgccagaac ggcttgtcgt acacggcggc gaccttcagc agcgtgccct
     4741 ggggcacgcg cgcgttcagc gcggcgcgct cggccggcag gtcggggctg aagtcgatct
     4801 ggcccgccag cgccgggggg atggccacga tcacgcgctt ggccttgacg atcaggcgcg
     4861 aggactccac gcgcacgccc ttcttgccct gcacgatctt cttgaccggc gagcgcagca
     4921 cgacgcgctt gcccagccgc ttgtgcagct cgtgggcgat ctgctgcgag ccgcccacga
     4981 agcgctgctc ctgcgcgccg tcgcgcgtgt tgaagttgcg ctcgaacgtg cccagcgtgc
     5041 gctcgttgcc cgaggaggcg atgtagtaca ggacgaacag gaggctcagc tcgcgcggct
     5101 cgccgccgaa gatcggccgg caggccaccg ccgccaggcg ccggaaccgc tccgacgtgc
     5161 tgttggcgtt cagccagctc tcgaaggtct ggccgtccca ctccttggcg cgcgcggcgc
     5221 gccacggggc gtcgggaggc acggtggccg ccatctcgtc cagcgccgcg atcacggtgg
     5281 ccaggtccag caggatggtc gggtccagcg gcgcggagcc ggtgggcagc gtgtcgctga
     5341 acgtgctgcg ctggccgtcg gccacgtaca cgttctcgcc gtcgttgaag gtcttgaagc
     5401 ggccgatgcc cagcgtgtcg gccagcttga tcacgtggtc ctgggtcggg ccgacgaacg
     5461 tgccgccgcg ctcggactcg atgcccttgc ccagctcgcg gttccacacc cggccgccca
     5521 cgcggtcacg cccctccagg acgcagaccg accgcccgtc gcgcaccagc tcgcgggccg
     5581 cggtcagccc ggcgaagccg gcgcccacga tcgccacgtc caccgtccgg gtgccccggc
     5641 cgcgcttgcg cttcttcttg cgcttcgcgg cctcggcgtc gtcgacgccg ggcagggcgg
     5701 ctgcggctgc ggtggcgcct gcggccactc ccgtgcccag gaaacgacgg cggctcagcg
     5761 gggcatcgct cacggtgctg cattctcctt cggtgggaag cgtcgtgcgg cagttcggag
     5821 gtaggcgcgg agcagctcct tgccctcgtc catcaggccc gggtccccgt cggggtccag
     5881 gcggaaggcc tcgtgcagca gggcgtcggc ggccagcacg ccggcgcggc aggcggtggc
     5941 cgcctcctcg gtgtcggcga tcacgcgccg gcccaccagg atccggcgca cgccgtcggc
     6001 caggatggcc ttgttggcgc ggtccgcggc ctggagctct tcgctgaact gccggcccag
     6061 ccacagcgcg cggtagcccg gctgcgacac gccccggtgc gcgaacacgt ccaggagcat
     6121 cccggccaga tcgtcccact cggcggtggg ggcggccgcg gccagctcgt cgatcgtggc
     6181 ctcgaactcg gccaggtagc ggcgggtcag ggcgtcgacg atcgccgcct tgtcggggaa
     6241 ccagcggtac agcgagccca ccgacacgcc cgcctcctcg gcgatccggg tggtggtcag
     6301 cgcctccggg ccctcccgga ccagcagctt ctcggcggcg tccagcagcc ctgccagccg
     6361 tgcgcggctg cgcgcctgct gtggctcgcg gcgcagcgtg ggcgagtccg aggtcgaggc
     6421 gggtggcgtc acgaccctcc atatacccac cccgccatga aagtgaactc accttcgcgt
     6481 ttctggtcgc gatcgggtac gttcccgctg tgggcgtccg ggaggcacgc gagcaggtgg
     6541 ccgccgtcgc gcggcggctg gccgccgagg gcctggtcct gggcacggcc ggcaacgtca
     6601 gccaacgcga cggggaccgc gtggccgtca cgccgaccgg cgccgtcctg gccgagctcg
     6661 aggccgccga cgtctgcgtc gtcgccctgg acggcagccc gatcgagggc ccgctggcac
     6721 cgacgtccga gctcgacctg cacctggggg cgatcaaccg ccacggctgc ggcgcggtgg
     6781 tgcacaccca cgcgcccatg tccaccgcgg cggcgtgcgt gctggacgag ctgccgtgca
     6841 tccactacgc gatgctcgcc ctgggcggcg acgtgccggt cgccccgtac accaccttcg
     6901 gcaccccgga gctggccgca tccgtggtcg acgcgctgga gggacgcacc gcggcgctca
     6961 tggccagcca cggcgcggtc accgtgggcg ccgacgccgc ggccgcgatg gacctggcgc
     7021 tgctcctgga gtgggcggcc accgtgtacc tcggcgccgc ccaggcgggc acgccgcacg
     7081 tgctcgacca ggccgagcgc acggccgtga tcgaggcggt cgtggccagc ggatacggaa
     7141 ccaccaaacg ggtcgacgac ggggaggcgc tatgagggcg atcgcgatgg gggtccacgt
     7201 ggccgacgtg ctggcgcggc ccgtggagga gatcccggcc ggccagggcg gggcactggt
     7261 ggacgagatc cggatcaccg ccgccgggtc ggccggcggc accgcgctga cgctggccaa
     7321 gctcggcggc gccgtcacca gcgcgggcgc catcggcacc gacgtcctgg gcgacgtgct
     7381 ggtgcgcctg ctcgaggacg gcggcgtcga cccttcgctg ctggtccgcc gcgacgacgt
     7441 ccagacctcg gccagcgtcc ttcccatccg ccccaacggc gaccgcccgg cgctgcacgt
     7501 cgtgggggcc aacggcacgt acgggcccga cgacgcgccc tgggacgcca tccgggacgc
     7561 gacccacctg cacctggggg cgcccgagtt catgggtggg gaggccgcgg cacggatcct
     7621 gtcctacgcc cgcgagcacg gcgtgaccac ctccgccgac atcctggccc cgggcgagca
     7681 ggcggcgcag atcgtggact ggatcgggcc cgccttcgcc cacctcgact acctgctgcc
     7741 caacgacgag caggtgctgg ccctgagcgg ccaggacgac ctcgagcggg gtgcgcgggt
     7801 gctgatggag cgcggcgtcg gctgcgtggc ggccacggcc ggcgcggacg gcgccatcgt
     7861 cgtgtcgccg gagggcaccg agcgggtccc ggcgttcccc gtggacgtgg tggacaccac
     7921 cggatgcggc gacgcgttca gcgcggggtt cctgcgcggc cggtcgctgg accgctcatt
     7981 gcgcgagagc gcggtgctgg gctgcgcggc cgccggcctc gtggcgggcg ggctgggctc
     8041 cgaccacggc gacttcgacc tggcggactg cgacgcgctg gccgccgggg cgtcggtcgg
     8101 ctagagcgcc agcgccagcg gctgctc
//
LOCUS       DASUPW010000352         2224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1192237, whole genome shotgun sequence.
ACCESSION   DASUPW010000352 DASUPW010000000
VERSION     DASUPW010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192237"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VFY44_07920"
     CDS             <1..541
                     /locus_tag="VFY44_07920"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HEX5910405.1"
                     /translation="EEPEGRELLRGVRDLGPAMDTFATVPPVALCELAMDPPEPLPVM
                     MTTAQLDAVPVEELTKAAGPGSGSPLAMIQIRQMGGALERTAPGAGARATLPGAYSLI
                     ALGVPENEGLAETIGGYLGAIDRAVEPHRCGEYPNFVERPADASAFFDADTWARLRAV
                     KGTYDPGELFRGNHYIPAA"
     gene            557..1027
                     /locus_tag="VFY44_07925"
     CDS             557..1027
                     /locus_tag="VFY44_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910406.1"
                     /translation="MKKTATALTVVATLALAGPATAKMQVYKGKTSTGHPVTAKIDKG
                     IIRYLVAGVSTTCLPIQSRGSSGRPSVAAEYFAVESTILKANKRHNRFQLQRKPAFHW
                     REVTTNHDIWLKRRKNRISGRVRLQYQYMVSTFPIGTFNIYSCLGSSTFTARLR"
     gene            complement(1031..>2224)
                     /locus_tag="VFY44_07930"
     CDS             complement(1031..>2224)
                     /locus_tag="VFY44_07930"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5910407.1"
                     /translation="RTAHFAERLESFARVLRYDKRGQGLSDRPGRPPTLEESMDDLRA
                     VMDAAGFQRAALFGVSEGGPMCQLFAATHPDRASALALYGTYARATRSDDYPIGFPRS
                     VMDTFNDAMLEGWGGPVGIELFAPSHAGDAEFRDWWARLLRSGTSPHGATALLDLYKE
                     LDTRAVLANIGVPTLVLQRRDDVLVREPQAAYLAEHIPGARYEPLDGSDHLVFAGDQD
                     AVLDHVEEFFTGTRRVPEVDRVLATVMFTDIVSSTRHAAELGDRRWRELLVRHDELVR
                     RQLDRHRGRAVKSLGDGFLATFDGPARAVRCAGAIRDDVRRLGVEIRAGLHTGECEVI
                     GDDVGGIAVHIGARVGARAEAGQVLVSNTVRDLVVGSGIEFAELGEHDLKGVPGRWRL
                     FAATV"
BASE COUNT          340 a          805 c          804 g          275 t
ORIGIN      
        1 cgaggagccc gagggacgcg agctgctgcg cggggtgcgt gacctggggc cggccatgga
       61 cacgttcgcc acggtgccgc ccgtcgcgct ctgcgagctg gccatggacc cgccggagcc
      121 gctgccggtg atgatgacca cggcccagct ggacgcggtg cccgtcgagg agctgacgaa
      181 ggcggccggc ccgggctcgg gctcgccgct ggcgatgatc cagatccggc agatgggcgg
      241 cgcgctggag cgcacggcac cgggcgccgg ggcgcgcgcc accctgcccg gcgcctacag
      301 cctgatcgcg ctgggggtgc cggagaacga ggggctggcc gagacgatcg gcggctacct
      361 gggcgccatc gaccgcgccg tggagccgca ccggtgcggc gagtacccga acttcgtcga
      421 gaggccggcc gacgccagcg ccttcttcga cgcagacacc tgggcgcgcc tgcgggccgt
      481 caagggcacg tacgaccccg gcgagctgtt ccggggcaac cactacatcc cggccgccta
      541 ggccagagag gacacgatga agaagacagc caccgcactc accgtggtcg ccacgctggc
      601 cctggccggc ccggcgacgg ccaagatgca ggtctacaag ggcaagacga gcaccgggca
      661 cccggtgacc gcgaagatcg acaaggggat catccgctac ctcgtggcgg gcgtcagcac
      721 cacctgcctg ccgatccaga gccgcggctc gagcggcagg cccagcgtgg ccgccgagta
      781 cttcgccgtg gagagcacga tcctgaaggc caacaagcgg cacaaccgct tccagctgca
      841 gcgcaagccg gcgttccact ggcgggaggt caccacgaac cacgacatct ggctcaagcg
      901 ccgcaagaac aggatctccg ggcgcgtgcg gctgcagtac cagtacatgg tgtcgacgtt
      961 cccgatcggc accttcaaca tctactcgtg cctgggcagc tcgacgttca cggcacgcct
     1021 gcgctgaagc tcagacggtg gcggcgaaca gccgccaccg tcccggcacg cccttgaggt
     1081 cgtgctcacc gagctcggcg aactcgatgc ccgagcccac gaccaggtcc cggacggtgt
     1141 tggacaccag cacctggccg gcctcggccc tggcccccac gcgggcgccg atgtggaccg
     1201 cgatgccgcc gacgtcgtct ccgatgacct cgcactcgcc cgtgtggagg ccggcgcgga
     1261 tctcgacgcc cagccggcgc acgtcatcgc ggatcgcgcc ggcgcaccgt acggcccggg
     1321 ccgggccgtc gaacgtggcc aggaagccgt cgcccagcga cttgaccgcc cggccacggt
     1381 ggcggtcgag ctggcggcgc accagctcgt cgtggcgcac cagcagctcg cgccagcgcc
     1441 ggtcgcccag ctcggccgcg tgccgggtcg acgagacgat gtcggtgaac atgaccgtgg
     1501 ccaggacgcg gtcgacctcc ggcacccgcc gggtgccggt gaagaactcc tcgacgtggt
     1561 ccaggacggc atcctggtcg cccgcgaaca ccaggtggtc gctgccgtcg aggggctcgt
     1621 agcgggcacc ggggatgtgc tcggccaggt acgccgcctg cggctcgcgc accaggacgt
     1681 cgtcccgccg ctggagcacc agggtcggca ccccgatgtt ggccagcacg gcgcgggtgt
     1741 ccagctcctt gtagaggtcc agcagggccg tggccccgtg ggggctggtg cccgagcgca
     1801 gcagccgcgc ccaccagtcg cggaactcgg cgtcgccggc gtgcgagggc gcgaacagct
     1861 cgatgcccac cggcccgccc cacccttcga gcatggcgtc gttgaacgtg tccatgaccg
     1921 agcgcgggaa gccgatcggg tagtcgtcgg acctggttgc cctggcgtag gtcccgtaga
     1981 gagccagcgc gctggcccgg tcggggtggg tggcggcgaa cagctggcac atcggaccgc
     2041 cctcggagac gccgaagagc gcggcccgct ggaacccggc cgcgtccatc acggcacgca
     2101 ggtcgtccat gctctcctcg agggtgggcg gccggccggg gcggtccgaa aggccctggc
     2161 cgcgcttgtc gtacctcagc acgcgtgcga acgactccag ccgctcggcg aagtgggccg
     2221 tgcg
//
LOCUS       DASUPW010000353         3929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_2023586, whole genome shotgun sequence.
ACCESSION   DASUPW010000353 DASUPW010000000
VERSION     DASUPW010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2023586"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..1848
                     /locus_tag="VFY44_07935"
     CDS             244..1848
                     /locus_tag="VFY44_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910408.1"
                     /translation="MADTRPGRVDWWRIAPTLLTTAFAIVYVIFQPRSPDLAAHIFRA
                     DLFGREGFTLWNGQWYGGHHTPAYSILSPPVVWALGPQVTGGLAAIGATVCFTELVRS
                     HFGPQRYRWGALWFALGTASLMFTNRIPFAMGVAFGMAACLALQRDRRVLAPLLAVLA
                     AISSPVAGLFVTLAGTAYALTASGEGVPLKRVDGALLAAGGLIPPVLLTLAFPEGGYA
                     PFPFTSYVTIPLACIAALFVLPRSEKALRAGAVLYALGATLAIILPTAMGGNAARLGL
                     LVAGPLLACSLSGRLRRPVTPIVVLLLALLIWQMSPAARDVYKAATDPVAKASYFDPV
                     REYLRLLPDQRRVEIPFTLGHWEGAEVGSDFPMARGWLRQLDTGRNPIFYKGDMSQVD
                     YANWLSQNAVRYVALPDAKPDNSAYREVALIERGLPYLRLRASFEHWRIYEVTLPAPV
                     VIPRGGADIQLEQLGSDQVLLRVRKPGSALVRVRWTPYWLAKGGCVERDGDWTRVTAT
                     RPGFLKLVTRFGPERVLQRGKRCNTG"
     gene            complement(1861..2538)
                     /locus_tag="VFY44_07940"
     CDS             complement(1861..2538)
                     /locus_tag="VFY44_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910409.1"
                     /translation="MRRLTLPLALLAVLAMAASALAATNAAKFDMGFTTKKPAKSTAF
                     SFDVAFANTGGNQVPAALNKFSIALPAGTKFNGDGAAKCTAAETELDTKFSAACPSAS
                     IVGSGKATAVPSGGGNSINTTVKIANQGKGSFRFFFALNGKDVTGFTAKAKGSKLTSE
                     TLTGSLPGGVIVTSLKGSIKKKSAKGKNLITTPKSCPKSKKWKFSGTFNFADGTHKVS
                     DSVSCKS"
     gene            2639..3451
                     /locus_tag="VFY44_07945"
     CDS             2639..3451
                     /locus_tag="VFY44_07945"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5910410.1"
                     /translation="MNQVAQDFFAQFRRYWNMTQRWLPQGWLDALRQLMLFAGAYYAY
                     RLVRGLVDGQAALAFENARTLVDVERGLGLFFEPGLQNWAQTNTQWLVDGANFMYVNS
                     HFVITTTFLIWLYIFRNRSYYYVRNMFMIAMFLALVGYVAYPTAPPRFMPEWGFTDTV
                     ASFVGQSAEQSANVLYNPFAAVPSMHVAFALMIGIPASQIVQHRVFKVLWLVYPAVVT
                     FVVVVTANHFWLDAALGALVAGLSASAASFALARARPDAWSWRAGRPAEVVA"
     gene            3456..>3929
                     /locus_tag="VFY44_07950"
     CDS             3456..>3929
                     /locus_tag="VFY44_07950"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX5910411.1"
                     /translation="MTGPAGAERVRGARRELARNRLIESRLTPNAISMTGLVGNIIAA
                     VLVTQEEFFLAGIAFILGSVCDTLDGRYSRMSGKGTPFGAFLDSSLDRIEEGIVLAAI
                     GVYFARIGDEPAVAAVVRAVLFSLMVSYTRARAEALGVECKVGLATRAIRVVIISA"
BASE COUNT          490 a         1415 c         1368 g          656 t
ORIGIN      
        1 ccatacgcct ggccaagccg cgctcgcgct gggcgcgccg cttctacgac gccgacaaga
       61 agcgcagggc cgaggcgcgc ttcgcctccg aacgccacga tcccccggtg actcccgccc
      121 ccgcgggcac gtagatccct ctaccctgag cgcgtgccgc gcgcggttct ctcagcagcg
      181 gcgcctaccc gcgcacgtcc ggcggagacg tcaggcgcca cgtcgcccgc ccccggggag
      241 cgggtggctg acacgcgccc gggacgggtg gactggtggc gcatcgcccc caccctgctg
      301 acgaccgcgt tcgcgatcgt ctacgtgatc ttccagccgc gctcgccgga cctggccgcc
      361 cacatcttcc gcgccgacct gttcggccgc gagggcttca ccctgtggaa cggccagtgg
      421 tacggcggcc accacacgcc cgcctactcg atcctgtcgc cgcccgtggt gtgggcactg
      481 ggcccgcagg tcaccggcgg cctggcggcc atcggcgcca cggtgtgctt caccgagctg
      541 gtgcggagcc acttcggccc ccagcgctac cgctggggcg cgttgtggtt cgccctcggg
      601 accgcctcgc tgatgttcac caaccggatc ccgttcgcca tgggcgtggc gttcggcatg
      661 gccgcctgcc tggcgctgca gcgcgaccgg cgcgtgctgg cccccctgct tgccgtgctg
      721 gccgccatct ccagccccgt cgcggggctg ttcgtgacgc tggcgggcac ggcctacgcg
      781 ctgaccgcct ccggcgaggg tgtcccgctg aagcgcgtcg acggcgccct cctggccgcc
      841 ggaggcctga tccccccggt gctgttgacg ctggccttcc ccgagggcgg ctacgccccg
      901 ttccccttca cctcgtacgt gacgatcccg ctggcgtgca tagcggcgct gttcgtgctc
      961 ccccggagcg agaaggcgct gcgggcgggc gccgtgctct acgccctcgg ggcgacgctg
     1021 gcgatcatcc tgcccaccgc gatgggcggg aacgccgccc gcctgggcct gctggtggcc
     1081 gggccgctgc tggcctgctc gctgtcgggc cggctgcgcc ggccggtcac gccgatcgtc
     1141 gtcctgctgc tcgcgctcct gatctggcag atgtccccgg ccgcgcgcga cgtgtacaag
     1201 gcggcgaccg acccggtggc caaggcctcc tacttcgacc cggtgcgcga gtacctgcgg
     1261 ctcctccccg accagcgccg ggtggagatc cccttcaccc tcggccactg ggagggcgcg
     1321 gaggtgggct cggacttccc gatggcgcgg ggctggctgc gccagctgga caccggccgc
     1381 aacccgatct tctacaaggg cgacatgagc caggtggact acgccaactg gctgtcacag
     1441 aacgccgtcc ggtacgtggc cctccccgac gccaagccgg acaacagcgc ctaccgcgag
     1501 gtcgccctga tcgagcgcgg cctgccctac ctgcgcctgc gggccagctt cgagcactgg
     1561 cgcatctacg aggtcacgct gccggcgccg gtggtgatcc cgcgcggggg cgcggacatc
     1621 cagctggagc agctgggctc ggaccaggtg ctgctgcgcg tgcgcaagcc cggctcggcg
     1681 ctggtgcgcg tgcgctggac gccctactgg ctggccaagg gcggctgcgt ggagcgcgac
     1741 ggcgactgga cgcgcgtgac ggccaccagg cccggcttcc tgaagctggt cacccgcttc
     1801 gggcccgagc gggtgctgca gcgcgggaag cgctgcaaca ccggctgagc cctgggcgtc
     1861 ctacgacttg cagctgacgc tgtcggagac cttgtgggtc ccgtcggcga agttgaacgt
     1921 cccggagaac ttccacttct tcgacttggg gcacgacttg ggcgtggtga tcaggttctt
     1981 gcccttcgcg ctcttcttct tgatcgaccc cttcagggag gtcacgatca cgccgccggg
     2041 caggctgccc gtgagcgtct cggaggtgag cttggagccc ttcgccttcg cggtgaaccc
     2101 ggtcacgtcc ttgccgttca gggcgaagaa gaaccggaac gagcccttgc cctggttggc
     2161 gatcttgacc gtggtgttga tcgagttgcc accgcctgag ggcaccgcgg tggccttgcc
     2221 gctgccgacg atcgacgccg acgggcaggc ggcgctgaac ttggtgtcca gctcggtctc
     2281 ggcggccgtg cacttggcag cgccgtcacc gttgaacttg gtcccggcgg ggagcgcgat
     2341 cgagaacttg ttcagggcgg cggggacctg gttgccgccg gtgttcgcga acgcgacgtc
     2401 gaacgagaac gcggtcgact tcgcgggctt cttggtcgtg aagcccatgt cgaacttggc
     2461 ggcgttggtc gcggccagag cggaggccgc catggccagc actgccagca gcgccagcgg
     2521 gagggtgagt ctgcgcatcg gggttccttt cgggacgggt cgtatcggag ccgttgccga
     2581 gcctaggtgt ggacagccga acccgccagc tccggccgag gggctacgct gtcccgtcgt
     2641 gaaccaggtg gcccaggact tcttcgccca gttccgccgg tactggaaca tgacccagcg
     2701 ctggctgccc cagggctggc tcgacgcgct gcgccagctg atgctgttcg cgggcgccta
     2761 ctacgcgtac cgcctggtgc gcggactggt cgacggacag gccgccctgg ccttcgagaa
     2821 cgcgcgcacg ctcgtggacg tggagcgtgg cctggggctg ttcttcgagc ccggcctcca
     2881 gaactgggcc cagaccaaca cgcagtggct cgtggacggg gcgaacttca tgtacgtgaa
     2941 ctcgcacttc gtgatcacga cgacgttcct gatctggctc tacatcttcc gcaaccgctc
     3001 ctactactac gtgcgcaaca tgttcatgat cgccatgttc ctggcgctcg tgggctacgt
     3061 ggcctacccc accgcgccgc cgcggttcat gccggagtgg ggcttcacgg acaccgtcgc
     3121 ctccttcgtg ggccagagcg ccgagcagag cgccaacgtg ctctacaacc ccttcgcggc
     3181 cgtgcccagc atgcacgtgg ccttcgcgct gatgatcggc atcccggcca gccagatagt
     3241 ccagcaccgc gtgttcaagg tcctgtggct ggtctacccc gcggtcgtga ccttcgtggt
     3301 ggtggtcacc gccaaccact tctggctgga tgccgccctc ggcgccctgg tcgccgggct
     3361 gtccgcgtcc gccgcatcgt tcgccctggc gcgcgcccgg cccgacgcct ggtcgtggcg
     3421 ggcgggccgg ccggccgaag tggtcgcctg accccatgac cggtcccgcg ggagccgagc
     3481 gagtacgcgg ggcgcggcgg gagctggcgc gcaaccggct gatcgagtcg cggctgacgc
     3541 ccaacgccat ctccatgacg gggctggtgg gcaacatcat cgccgccgtg ctggtgaccc
     3601 aggaggagtt cttcctggcc gggatcgcgt tcatcctggg ctcggtctgc gacaccctcg
     3661 acgggcgcta ctcgcgcatg tcgggcaagg gcaccccgtt cggcgccttc ctggactcca
     3721 gcctggaccg gatcgaggag ggcatcgtgc tggccgcgat cggcgtgtac ttcgcccgga
     3781 tcggcgacga gcccgcggtg gccgccgtcg tgcgggccgt gctgttcagc ctgatggtct
     3841 cctacacccg ggcgcgcgcg gaggcgctgg gggtcgagtg caaggtgggc ctggccacgc
     3901 gcgcgatccg cgtggtcatc atctcggcc
//
LOCUS       DASUPW010000354         3869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1884852, whole genome shotgun sequence.
ACCESSION   DASUPW010000354 DASUPW010000000
VERSION     DASUPW010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1884852"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VFY44_07955"
     CDS             complement(<1..429)
                     /locus_tag="VFY44_07955"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX5910412.1"
                     /translation="MTVAVVGHVEWADAVRVERLPTAGQIVHGEPAWAAPAGGGGVTA
                     VVLGRLAGQVHLFTALGDDDLGRRSAAALGDLGVTVHAAWRDHPQRKALVHVDGDGER
                     AITVVGTRAGPAGEDDLPWDLLDRADAVYFTAGDPGAVAHA"
     gene            454..867
                     /gene="arfB"
                     /locus_tag="VFY44_07960"
     CDS             454..867
                     /gene="arfB"
                     /locus_tag="VFY44_07960"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEX5910413.1"
                     /translation="MQDPMPIDSHTAIPLAEVELRTSRSSGPGGQHANVTASRVEAIF
                     DAAASPSLSESQRRRVIARAGARVTAVAQDTRSQARNRELALERLQSRLAAALRQQRR
                     RRSTRPTRASKERRLEGKRQRSNVKRARKRPDADS"
     gene            complement(868..1305)
                     /locus_tag="VFY44_07965"
     CDS             complement(868..1305)
                     /locus_tag="VFY44_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910414.1"
                     /translation="MRRRLVAALAAGIVLIVIIALVAGGGGDDPKKSSSPAELEVPTT
                     PEVESEPTDTTDSTNTTTSPTPSGTAGGGTSGGTTGGTTGGTTGGGTTGGTTGGGTTG
                     GGTTGGGTTGGGGTTGGGTGTPQAPNQTPPASGGGGAVAPNSP"
     gene            complement(1456..2223)
                     /locus_tag="VFY44_07970"
     CDS             complement(1456..2223)
                     /locus_tag="VFY44_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910415.1"
                     /translation="MRVPAAICAVAAAAVVAAGCGREQQKQADDPVDRDKADVVLTPE
                     SVDPDAVRRAEQKASKSQARAVKLEGAGAKKTYDGGEGISPGAPTDDEVKADLKEARS
                     ELRKFKKLLSTTAYLQTGPRARVLKDGTAVAPEDAPEPVKRVIQAGNAISKLPYKWGG
                     GHGAWRDTGYDCSGSVSFALAGAGLLRSPLASGPFMKWGDAGEGEWITIYANPGHIFM
                     VVAGLRFDTSGRGRAGTRWQEASRGTGGFAVRHFPGL"
     gene            2268..2555
                     /locus_tag="VFY44_07975"
     CDS             2268..2555
                     /locus_tag="VFY44_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910416.1"
                     /translation="MSDRPNPRLPEDKSGIDQPHDVLAADEFAVPGRSVGDVPSDPIH
                     TPHDVLAAEEFAIPGGGAGGGGRSGLDPRTFLPSLLLVATILLLVRRHRQG"
     gene            complement(2546..2782)
                     /locus_tag="VFY44_07980"
     CDS             complement(2546..2782)
                     /locus_tag="VFY44_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX5910417.1"
                     /translation="MSEELSPVNRIRIEVDRGLCIGSGDCVDSAPGVFKLDQEDKAVV
                     IDPDGDPLDMVMDAAGNCPVTAIFIYGEEGDLYP"
     gene            complement(2802..3410)
                     /locus_tag="VFY44_07985"
     CDS             complement(2802..3410)
                     /locus_tag="VFY44_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910418.1"
                     /translation="MRRLLPAVLACLSLFALTAVAAVATPYGRVAHNPADRLAAKPID
                     DYRYDRAKHCYRRPSRGAGALVAWLGRNARGQFWGIMRCERLSRDSMSLHSEGRAIDW
                     HLDVSSAADRRAARRLILTLLATDRAGNPHALARRMGVQEIIWNCRSWWSGADRMGPY
                     SLCYTRKGKLRRKVNRTLAHRDHVHIGLSRSGARMRTSFWRR"
     gene            complement(3446..>3869)
                     /locus_tag="VFY44_07990"
     CDS             complement(3446..>3869)
                     /locus_tag="VFY44_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5910419.1"
                     /translation="SMKIMTEETFGPTLPIMKVKDSEEALALANDSEYGLQASVWTKD
                     ADRGSQLAARLEAGAANVNEHQLNYLALEAPMGGWKSSGLGTRHGKQGIRKYTRQQVS
                     VIAARGMKKELFMYPYKARTTKLLGGLLKLLNGRGKSS"
BASE COUNT          471 a         1455 c         1389 g          554 t
ORIGIN      
        1 ggcgtgcgcc acggcgccgg ggtcgccggc ggtgaagtac accgcgtcgg cccggtccag
       61 caggtcccag ggcaggtcgt cctcgcccgc cgggccggca cgcgtgccca ccaccgtgat
      121 cgcccgctcg ccgtcaccgt ccacgtgcac cagggccttg cgctggggat ggtcacgcca
      181 ggcggcgtgc accgtcaccc ccaggtcgcc cagggccgcg gccgagcgcc ggcccaggtc
      241 gtcgtccccc agggcagtga acaggtgcac ctgcccggcc aggcggccca gcaccaccgc
      301 ggtcacgcca ccgccgccgg cgggcgcggc ccaggccggc tcgccgtgga cgatctggcc
      361 cgccgtcggc aggcgctcga cgcgcacggc gtcggcccac tccacatgtc ccaccacggc
      421 gaccgtcacg ggcgccagtg taggttcgcc gccatgcagg acccgatgcc catcgattcc
      481 cacaccgcga tcccgctggc ggaggtcgag ctgcgcacca gccgctcgtc cggccccggc
      541 ggccagcacg ccaacgtcac ggcctcgcgg gtggaggcga tcttcgacgc cgccgcctca
      601 ccgtcgctga gcgagtcaca gcggcggcgg gtgatcgccc gcgccggcgc gcgcgtcacg
      661 gccgtggcgc aggacacccg cagccaggcc cgcaacaggg agctggcgct ggagcggctc
      721 cagtcccgcc tggccgccgc gctgcgccag cagcgccgcc gccgcagcac gcggccgacg
      781 cgcgcgtcga aggaacgccg cctggagggc aagcgccagc gcagcaacgt gaagcgggcc
      841 cgcaagcggc ccgacgcgga ctcctgacta cgggctgttc ggcgccacgg cgccgccgcc
      901 acccgatgcg gggggcgtct ggttgggcgc ctggggcgtg ccggtgccgc cgccggtggt
      961 cccaccgccg ccggtggtcc ccccgcccgt ggtgccgccg cccgtggtgc cgccgccggt
     1021 cgtgccgccg gtggtcccgc cgccggtcgt gccgcccgtg gtgccgccgg tcgtgccgcc
     1081 gcttgtgccc ccgcccgcgg tgcccgaggg cgtcgggctg gtggtggtgt tggtggagtc
     1141 ggtggtgtcg gtcggctcgc tctcgacctc gggcgtggtg ggcacctcga gctcggccgg
     1201 gctggacgac ttcttggggt cgtcgccccc gccgccggcc accagggcga tgatcacgat
     1261 gagcacgatc ccggcagcca gagcggccac caggcggcgc cgcatgtaga ccgagtcgtc
     1321 ctcgcgctgg ggcggtcggc gccgcggatc gcgggcaggc tctaggcgtc gtgtcggctg
     1381 gtcgtccaca gtgcggcgaa ggtagcagtc acctcgcagg ttccctgcac caccgggaat
     1441 ccctgcacag agggcctaca ggccggggaa gtggcgcacc gcgaagccgc ccgtgccgcg
     1501 ggacgcctcc tgccagcggg tgcccgcgcg gccccgtcca ctggtgtcga agcgcagccc
     1561 ggccaccacc atgaagatgt gcccggggtt ggcgtagatg gtgatccact ccccctcccc
     1621 ggcatccccc cacttcatga acgggccgga cgccagcggg ctgcgcagca gaccggcgcc
     1681 ggccagcgcg aacgacaccg agcccgagca gtcgtagccg gtgtcgcgcc aggcgccgtg
     1741 gcctccgccc cacttgtagg gcagcttgga gatggcgttg ccggcctgga tcacgcgctt
     1801 gaccggctcg ggcgcgtcct cgggtgccac ggcggtgccg tccttgagca cccgcgcgcg
     1861 cggcccggtc tgcaggtagg cggtggtgct cagcagcttc ttgaacttgc gcagctcgct
     1921 gcgtgcctcc ttgaggtcgg ccttgacctc gtcgtcggtg ggcgcgcccg ggctgatgcc
     1981 ctcgccgccg tcgtaggtct tcttcgcgcc ggcgccctcc agcttcaccg cgcgggcctg
     2041 cgacttgctg gccttctgct ccgcgcgccg gaccgcgtcg ggatccacgc tctcgggcgt
     2101 gagcaccacg tccgccttgt cccggtcgac cgggtcatcc gcctgcttct gctgctcccg
     2161 gccgcagccg gcggccacga ccgcggccgc cgcgacggca cagatggctg cggggaccct
     2221 catcagcgga aacgtagcgg gacgagcggt ctggcagtat caggtcgatg agcgatcgac
     2281 ccaacccgcg gctgcccgag gacaagagcg ggatcgacca gccgcacgac gtcctggccg
     2341 ccgacgagtt cgccgttccc ggccgctcgg tcggcgacgt gccctccgac ccgatccaca
     2401 caccccacga cgtgctggcc gcggaggagt tcgccatccc gggcggcggt gccggcgggg
     2461 gtggccgcag cggcctggat ccccggacct tcctgccctc gctgctgctg gtggcgacga
     2521 tcctgctgct ggtgcgccgc catcgtcagg ggtagaggtc gccctcttcg ccgtagatga
     2581 agatcgcggt cacggggcag ttgcccgccg cgtccatgac catgtccagg ggatccccgt
     2641 cggggtcgat gaccaccgcc ttgtcctcct ggtcgagctt gaacacgccc ggggcggagt
     2701 ccacgcagtc ccccgagccg atgcagaggc cgcgatccac ctcgatgcgg atccggttga
     2761 cgggtgagag ttcctcgctc atgagcgggc caggctagtg actagcggcg ccagaagctc
     2821 gtgcgcatgc gcgcgccgct gcggctgagc ccgatgtgca cgtggtcgcg gtgggccagc
     2881 gtcctgttca ccttcctgcg cagcttgccc tttcgcgtgt aacagagcga gtacggcccc
     2941 atccggtccg cgcccgacca ccacgagcgg cagttccaga tgatctcctg cacgcccatc
     3001 cgccgggcca gggcgtgcgg gttgccggcg cggtcggtgg ccagcagggt caggatcagg
     3061 cgccgggccg cacgccggtc ggcggcgctg gacacgtcca ggtgccagtc gatggcacgc
     3121 ccctcggagt gcaggctcat gctgtcgcgg gacagccgct cgcagcgcat gatgccccag
     3181 aactggccgc gggcgttgcg gcccagccag gccaccaggg cgccggcgcc ccggctgggg
     3241 cgccggtagc agtgcttggc ccggtcgtag cggtagtcgt cgatgggctt cgcggccagc
     3301 cggtccgccg ggttgtgggc cacgcggccg tagggggtcg ccaccgccgc cacggcggtc
     3361 agggcgaaca ggctcaggca ggccaggacg gcggggagca atcggcgcac ccccgccttt
     3421 tcggcagctc aggccaaagg ttgagtcagc tcgacttgcc gcggccgttc agcagcttca
     3481 gcagcccgcc cagcagcttc gtggtgcgcg ccttgtacgg gtacatgaag agctccttct
     3541 tcatcccgcg cgcggcgatc accgacacct gctggcgcgt gtacttgcgg atgccctgct
     3601 tgccgtggcg ggtgcccagg cccgaggact tccagccgcc catcggcgcc tcgagcgcca
     3661 ggtagttcag ctggtgctcg ttcacgttcg ccgcgccggc ctcgagccgg gcggccagct
     3721 gggagccgcg gtcggcgtcc ttggtccaca ccgacgcctg gaggccgtac tcggagtcgt
     3781 tggccagcgc cagcgcctcc tcggagtcct tgaccttcat gatcggcagc gtcgggccga
     3841 aggtctcctc ggtcatgatc ttcatcgag
//
LOCUS       DASUPW010000355         4216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_361700, whole genome shotgun sequence.
ACCESSION   DASUPW010000355 DASUPW010000000
VERSION     DASUPW010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361700"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VFY44_07995"
     CDS             <1..427
                     /locus_tag="VFY44_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910420.1"
                     /translation="AAVALVLALQLAGLREELGRTALQVDRETFVLRDVARTLPPGSI
                     RVDVPPSGRHLWASYYLNERRLSSYTPVLYTTYPHVPGGRKADFILADRTLKLKRWPD
                     ADGPPIWQNRIWKIYRMKPGVPGPDRSSLRMQDDFGGTF"
     gene            complement(424..1143)
                     /locus_tag="VFY44_08000"
     CDS             complement(424..1143)
                     /locus_tag="VFY44_08000"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5910421.1"
                     /translation="MSEEYDPDNLLDRNTPQATMEVHYEQVRRREAAILAGRLPLTGG
                     DVLSVGCGWNPGRHMFPSPAWRMTGVELEPTKPDALVADGILDAGFAGRAGDLGLEPG
                     SFDVVLYRLVLHHIAYQGPLAPVFDEAAALLRPGGALIAVEPGAWHPVGAGLSLANRA
                     GAGTAVHGTPDDIPLSPRRLMAEARAAGLEPELHGVTYTWRRMPPALQRALWAVDSGL
                     GDRPRTAGLGHTLMLIARRPG"
     gene            complement(1140..2168)
                     /locus_tag="VFY44_08005"
     CDS             complement(1140..2168)
                     /locus_tag="VFY44_08005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5910422.1"
                     /translation="MTAVHQVLSSAGPYDAVSVQARLWRELLTERGYGGADHAAAVDP
                     RARGDFAPLERLRPRRGDLLVIRWSAYSPRLLALLERPERKLLVYHNVTPPGYFWNHH
                     PGVAVACAVGRAQLPLFARAVDVCAADSDFNAGDLRAAGADARVLPILFDPSRLEERG
                     RAPDGDGPLVLVVSRLAPNKRHDLAIAAFDAWRAEHGGPARMLCVGEPLSPSYAELIG
                     GLANGSVTLAGGLSQPDLNAAYAGADAMLSMSEHEGFSVPLLEAFHFGLPVVARPAGA
                     MPEVGGDAVLWDRDDDLAVTAELIGAAVADEALRATLAERGRARLAEFDHARTADKID
                     AAIQDALR"
     gene            complement(2165..2500)
                     /locus_tag="VFY44_08010"
     CDS             complement(2165..2500)
                     /locus_tag="VFY44_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910423.1"
                     /translation="MSTPEDAAARAREEAERRRAAGGYAGVGSHGPLDESIVHGAPSR
                     ELLAQWAVIEIDADEILYSTRRGGAPITFFKRILVRLLRQYFVELEARQTRFNIALLE
                     RLDALEKKQ"
     gene            complement(2497..3687)
                     /locus_tag="VFY44_08015"
     CDS             complement(2497..3687)
                     /locus_tag="VFY44_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5910424.1"
                     /translation="MKPGEIVLEGATRRFKLTHERSRTLKELFVSRGGAGAGSIAALD
                     GVDLRIEPGDAVGLIGRNGAGKTSTLRCLAGIVPLDSGSAECGGRTVSLLELGAGFGP
                     DFSGRENIYLNGALHGFGRGEMEERVEQMIDFSELGDFIDVPVKAYSSGMQLRLGFSI
                     VAHLDADVMLIDEILAVGDESFQRKCMARIAERMAAGTTLVLVSHDPSAIERICRRVV
                     VLDDGKVAFDGDTADGLLFYHRLLGTGEGRSRSLRPDAAERAQLEIAELELVDAGGQP
                     RAAFRTGEELGVRMSVRALEPAERAVVALEVRDQRGEVCFRTDETVGELAGETVVSFA
                     VPRLNLLGGDYDVAVGAYDQDAPASGLLDRVARFSVAHTTDGEGVADLRGTWSLVGRR
                     EAVR"
     gene            complement(3684..>4216)
                     /locus_tag="VFY44_08020"
     CDS             complement(3684..>4216)
                     /locus_tag="VFY44_08020"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5910425.1"
                     /translation="VQNAPLVRKVRFPRQTIPASVATVQLATFLVMLVVVTPVALVVR
                     GSADPALALLVPIVALLFCLVLGLSLIVAVLHAHYRDVEPVLATALLPWFFLTPIFLP
                     IDSMPGIADHAWVGDVLRWGNPIAPFIEATRDVLYAGTTPSGGTLVYVLVAGLGALGL
                     GTLLFRRLERDLAVIL"
BASE COUNT          588 a         1658 c         1470 g          500 t
ORIGIN      
        1 ggcggcggtc gcgctggtgc tggcgctgca gctggccggg ctgcgcgagg agctcgggcg
       61 caccgcgctg caggtggacc gcgagacgtt cgtgctgcgc gacgtcgccc gcacgctccc
      121 gccgggcagc atccgcgtgg acgtcccgcc gagcggacgc cacctgtggg cgtcctacta
      181 cctgaacgag cgccggctga gctcctacac cccggtcctc tacaccacct atccgcacgt
      241 ccccggtggc cgcaaggccg acttcatcct ggccgacagg acgctgaagc tcaaacggtg
      301 gcccgatgcg gacgggccgc cgatctggca gaaccggatc tggaagatct accggatgaa
      361 gcccggcgtg cccggcccgg accggtcgtc gctgaggatg caggacgact tcggcggcac
      421 gttctagccc gggcggcggg cgatgagcat cagggtgtgg cccagccccg cggtcctcgg
      481 gcggtcaccc agccccgagt ccacggccca cagcgcgcgc tgtagcgccg gcggcatccg
      541 ccgccacgtg taggtcaccc cgtgcagctc gggctccagg cccgccgcgc gcgcctccgc
      601 catcagccgc cgcggcgaca gcgggatgtc gtcgggcgtg ccgtgcacgg cggtgccggc
      661 gccggcccgg ttggccagcg acagccccgc gcccacgggg tgccaggcgc ccggctccac
      721 cgcgatcagc gcgcccccgg gacgcagcag cgccgcggcc tcgtcgaaca ccggcgccag
      781 cggcccctgg taggcgatgt ggtgcagcac caggcggtag agcaccacgt cgaaggagcc
      841 gggctccagc ccgaggtcgc ccgcgcgccc ggcgaagccg gcgtccagga tgccgtcggc
      901 caccagcgcg tccggcttgg tcggctccag ctccacgccg gtcatgcgcc acgccgggct
      961 ggggaacatg tgccggcccg ggttccagcc gcaccccacg gagagcacgt cgcctcccgt
     1021 cagcggcagc cgcccggcca ggatggccgc ctcgcgccgg cgcacctgct cgtagtgcac
     1081 ctccatcgtg gcctggggcg tgttgcggtc cagcaggttg tcggggtcgt actcctcgct
     1141 catctcaggg catcctggat cgccgcgtcg atcttgtccg cggtgcgggc gtggtcgaac
     1201 tccgcgagcc gtgcccgtcc gcgctcggcc agcgtggccc gcaacgcctc gtcggccacc
     1261 gccgcgccga tcagctcggc ggtgaccgca aggtcgtcgt cgcgatccca cagcaccgcg
     1321 tcaccgccta cctcgggcat cgcccccgcg ggccgcgcca ccacgggcag gccgaagtgg
     1381 aaggcctcca gcagcggcac cgagaacccc tcgtgctcgg acatcgacag catcgcgtcg
     1441 gccccggcgt aggcggcgtt caggtccggc tgggacagcc cgccggccag cgtgaccgac
     1501 ccgttggcca gcccgccgat gagctcggcg taggagggcg acagcggctc gccgacgcac
     1561 agcatccggg ccgggccgcc gtgctccgcg cgccaggcgt cgaacgcggc gatggccagg
     1621 tcgtggcgct tgttcggagc cagccggctg accaccagca ccagcggccc gtcgccatcg
     1681 ggcgcgcgcc cgcgctcctc cagccgcgac gggtcgaaca gtatcggcag cacccgcgcg
     1741 tcggcgcccg cggcccgcag gtcgccggcg ttgaagtccg agtcggccgc acacacgtcc
     1801 actgcgcggg cgaacagcgg cagctgcgcg cggcccaccg cgcaggccac ggcgacgccg
     1861 gggtggtggt tccagaagta gcccggcggc gtgacgttgt ggtacacgag cagcttgcgc
     1921 tcggggcgct ccagcagcgc cagcagccgc ggcgagtacg ccgaccagcg gatcaccagc
     1981 aggtcgcccc gcctgggccg cagccgctcc agcggcgcga agtcgccacg cgcccgcggg
     2041 tccaccgccg cggcgtggtc ggcgccgccg tacccgcgct cggtcagcag ctcgcgccac
     2101 agccgggcct gcacgctgac cgcgtcgtag ggccccgcgc tggacaggac ctggtggacc
     2161 gcggtcactg cttcttctcg agcgcatcca ggcgctcgag gagggcgatg ttgaaccgcg
     2221 tctggcgcgc ctccagctcc acgaagtact ggcgcagcag ccgcaccagg atccgcttga
     2281 agaaagtgat cggcgccccg cccctgcgcg tcgagtacag gatctcgtcg gcgtcgatct
     2341 cgatcaccgc ccactgcgcc agcagctccc gcgacggagc gccgtgcacg atcgactcgt
     2401 ccagcgggcc gtggctgccc acgcccgcgt agccccccgc cgcccggcgg cgctcggcct
     2461 cctcgcgtgc gcgcgcggcc gcgtcctcgg gcgtgctcac ctgaccgcct cccgccgtcc
     2521 caccagcgac cacgtgccgc gcaggtcggc cacgccctcg ccgtcggtgg tgtgcgcgac
     2581 cgagaaccgc gcgacgcggt ccaggagccc gctggccggc gcatcctggt cgtaggcgcc
     2641 gaccgccacg tcgtagtcgc cgcccagcag gttcagccgc gggacggcga acgacaccac
     2701 cgtctcgccc gccagctcgc cgacggtctc gtccgtgcgg aagcacacct cgccgcgctg
     2761 gtcgcgcacc tccagcgcca ccaccgcgcg ctcggccggc tccagcgcgc gcacgctcat
     2821 ccgcacgccc agctcctcgc cggtgcggaa cgcggcacgc ggctgcccgc cggcgtcgac
     2881 cagctccagc tcggcgatct ccagctgggc gcgctcggcc gcgtccgggc gcagcgaccg
     2941 cgaccggccc tcgccggtgc ccagcagccg gtggtagaac agcagcccgt cggcggtgtc
     3001 gccgtcgaag gccaccttgc cgtcgtccag caccaccacc cggcggcaga tgcgctcgat
     3061 ggccgacggg tcgtgcgaga ccagcaccag cgtggtgccg gcggccatcc gctccgcgat
     3121 ccgggccatg cacttgcgct ggaacgactc gtcgcccacg gccaggatct cgtcgatgag
     3181 catcacgtcg gcgtccaggt gggccacgat cgagaagccc agccgcagct gcatgcccga
     3241 cgagtaggcc ttgaccggca cgtcgatgaa gtcgcccagc tccgagaagt cgatcatctg
     3301 ctcgacgcgc tcctccatct ccccgcggcc gaagccgtgc agggcgccgt tgaggtagat
     3361 gttctcgcgc ccgctgaagt ccgggccgaa cccggcgccc agctccagca gcgacacggt
     3421 ccgcccgccg cactcggcgc tgccggagtc cagcggcacg atcccggcca ggcagcgcag
     3481 cgtcgaggtc ttgcccgcgc cgttgcggcc gatcaggccg acggcgtcgc cgggctcgat
     3541 ccgcaggtcg accccgtcca gcgcggcgat cgagcccgcc cccgcgcccc ctcgggagac
     3601 gaacagctcc ttcagcgtgc gcgagcgctc gtgggtcagc ttgaagcggc gggtcgcgcc
     3661 ctcgagcacg atctccccgg gcttcacagg atcaccgcca ggtcgcgctc gagccgccgg
     3721 aacagcagcg tgcccaggcc cagggcgccc aggccggcca ccagcacgta gaccagcgtg
     3781 ccccccgacg gcgtcgtgcc cgcgtagagc acgtcacggg tggcctcgat gaacggggcg
     3841 atggggttgc cccagcgcag cacgtcgccc acccaggcgt ggtcggcgat gccgggcatg
     3901 gagtcgatgg gcaggaagat cggcgtcagg aagaaccacg gcagcagcgc cgtggccagc
     3961 accggctcga cgtcgcggta gtgggcgtgc aggacggcca cgatcagcga caggcccagc
     4021 accaggcaga acagcagcgc cacgatcggc acgaggagcg ccagcgccgg gtccgccgag
     4081 ccgcgcacca ccagcgccac cggcgtgacc accaccagca tcaccaggaa cgtggccagc
     4141 tggaccgtgg ccaccgacgc cgggatggtc tggcgcggga agcgcacctt gcgcaccagc
     4201 ggggcgttct gcacca
//
LOCUS       DASUPW010000356         2331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1053549, whole genome shotgun sequence.
ACCESSION   DASUPW010000356 DASUPW010000000
VERSION     DASUPW010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053549"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VFY44_08025"
     CDS             <1..316
                     /locus_tag="VFY44_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910426.1"
                     /translation="MAVAATVTEQDAAFARDVGAEAARQIRAIARPGTVIALPTVPCA
                     PPRIDAGPDEVESFRARTLRLTCIAGLSGLPQVTVPAGLSLIGWPGGDESLLEVAAEL
                     TG"
     gene            complement(313..1293)
                     /locus_tag="VFY44_08030"
     CDS             complement(313..1293)
                     /locus_tag="VFY44_08030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEX5910427.1"
                     /translation="MRALQISELTGPRGLTVTELPDPGPEHPMTPGEGVLIDVHAAGA
                     AFPDVLQTRGEYQFKPPLPFVPGAEVAGVVREASDGAGVSVGDRVAACTYLGGFADVA
                     VAPAHMTFPLDDALDFEQGASLVFNYHTVYFALKIRGRLQAGERVLVHGAAGGVGSAA
                     LQVAKGLGADTVAVVSSDEKAAVAERAGADLVVRSDRAWKDEVKESGGVDVVLDPVGG
                     DRFTDSLRCLREAGRVVVVGFTGGSIPEVKVNRLLLNNTEVIGAGWGAWVMPKPQASI
                     EIGAELDKLIASGAVRPIVGASFPLEQGPEALELIDGRGATGKVVLTTGR"
     gene            complement(1322..1663)
                     /locus_tag="VFY44_08035"
     CDS             complement(1322..1663)
                     /locus_tag="VFY44_08035"
                     /inference="COORDINATES: protein motif:HMM:NF036395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase family protein"
                     /protein_id="HEX5910428.1"
                     /translation="MTTQTRTASEQITDEVTSWPGVTASYGDRSEFSFKLGRREIGHL
                     HGDHALHLGFPKAVWHELYDQGRIDYHPVVPDKPGFAARRIETQEDVDDAIALLRINY
                     DRAVSTHGLPD"
     gene            1745..2086
                     /locus_tag="VFY44_08040"
     CDS             1745..2086
                     /locus_tag="VFY44_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5910429.1"
                     /translation="MESWQLSDEPLEPHHPRILHTDPGANRVIALALPAGEMLQDHEV
                     RERALVFVVEGEAVVRCDGLEERLHAPALMHFDASERHEVEAVTDVRLTLCLAPWSEG
                     YHDRHPSDPQA"
     gene            complement(2083..>2331)
                     /locus_tag="VFY44_08045"
     CDS             complement(2083..>2331)
                     /locus_tag="VFY44_08045"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF014820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX5910430.1"
                     /translation="DLFEVNEAFAPVVLAWQAETGASLDKVNVNGGAIALGHPLGASG
                     ARLTTTLVNALRNRDQRWGLQTMCEAGGLANAMVVEAL"
BASE COUNT          305 a          857 c          840 g          329 t
ORIGIN      
        1 gatggctgtg gcggcgacgg tgaccgagca ggacgccgcg ttcgcgcgtg acgttggggc
       61 cgaggccgcc cgccagatcc gcgccatcgc ccggcccggc acggtgatcg cgctccccac
      121 cgttccgtgc gcgccgccgc ggatcgacgc cgggcccgac gaggtggagt cgttccgcgc
      181 ccggacgctg cggctgacct gcatcgcggg gttgtcgggc ctgccgcagg tgaccgtccc
      241 ggccgggctc tcgctgatcg ggtggcccgg cggcgacgag tcgctgctgg aggtcgcggc
      301 cgagctgacc ggctagcggc ccgtggtcag cacgaccttg ccggtggcgc cgcgaccgtc
      361 gatcagctcc agcgcctccg ggccctgctc cagcgggaac gaggcgccca cgatcgggcg
      421 cacggcgccg gaggcgatca gcttgtccag ctcggcgccg atctcgatcg aggcctgcgg
      481 cttgggcatg acccacgccc cccagccggc gccgatgacc tcggtgttgt tcagcagcag
      541 ccggttgacc ttgacctcgg ggatcgagcc gccggtgaag cccacgacca ccacgcggcc
      601 ggcctcgcgc agacagcgca ggctgtcggt gaagcggtcg ccgcccaccg ggtccaggac
      661 gacgtccacg ccgccggact ccttgacctc gtccttccac gcacggtcgg atcgcaccac
      721 caggtccgcg cccgcccgct cggccacggc cgccttctcg tcggaggaga ccaccgcgac
      781 ggtgtcggcg cccaggccct tggccacctg cagcgcggcc gacccgacgc cgccggcggc
      841 cccgtgcacg agcacacgct cgccggcctg cagccggccg cggatcttca gcgcgaagta
      901 caccgtgtgg tagttgaaga ccaggctggc gccctgctcg aagtccagcg cgtcgtccag
      961 agggaaggtc atgtgggccg gggcgaccgc cacgtcggcg aagccgccca ggtaggtgca
     1021 cgcggccacg cggtcgccca cggacacgcc cgcgccgtcc gaggcctcac ggacgacccc
     1081 cgccacctcg gcgccgggca cgaacggcag cggcggcttg aactggtact cgccccgggt
     1141 ctggagcaca tcggggaacg ccgcgccggc cgcgtgcacg tcgatcagaa cgccctcgcc
     1201 gggcgtcatc gggtgctcgg ggcccggatc gggcagctcg gtgacggtca ggccgcgggg
     1261 gccggtcagc tcggagatct ggagcgcgcg catgcgcggg acattcgcat acgcgcgcgc
     1321 ctcagtcggg caatccgtgc gtggagaccg cgcggtcgta gttgatccgc agcagcgcga
     1381 tcgcgtcgtc cacgtcctcc tgcgtctcga tccgccgcgc ggcgaacccg ggcttgtccg
     1441 ggaccaccgg gtggtagtcg atccgcccct ggtcgtacag ctcgtgccac acggccttgg
     1501 ggaagccgag gtgcagcgcg tggtcgccgt gcaggtggcc gatctcacgc ctgccgagct
     1561 tgaacgagaa ctcgctgcgg tcgccgtagg aggcggtcac gcccggccac gaggtgacct
     1621 cgtcggtgat ctgctcgctt gccgtcctgg tctgggtggt catggaggga ccgtaggcct
     1681 tcaagcgcgg ttgaagtcaa gggtttcgcg aaccggccgc gtgtcgtggc gtaggctggc
     1741 gcccatggag tcctggcagc tgtccgacga gccgctcgag ccgcaccatc cgcggatcct
     1801 gcacaccgac cccggcgcga accgggtgat cgcgctggcc ctgccggccg gcgagatgct
     1861 ccaggaccac gaggtgcgtg aacgcgcgct cgtgttcgtg gtcgagggcg aggcggtcgt
     1921 tcgctgcgac ggcctcgagg agcgcctgca tgcgccggcg ctgatgcact tcgacgcgtc
     1981 cgagcgccac gaggtcgagg cggtcaccga cgtgcgcctg accctgtgtc tggcgccgtg
     2041 gtccgaggga taccacgacc ggcatccctc ggaccctcag gcctagagcg cctcgacgac
     2101 catcgcgttg gcgaggccgc cggcctcgca catcgtctgc aggccccagc gctggtcccg
     2161 gttgcgcagg gcgttgacca gcgtcgtggt cagccgcgcg ccgctggcgc ccagcgggtg
     2221 gcccagggcg atcgcgccgc cgttcacgtt gaccttgtcc agcgacgcgc cggtctcggc
     2281 ctgccacgcc agcacgaccg gtgcgaacgc ctcgtttacc tcgaacaggt c
//
LOCUS       DASUPW010000357         5308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_776174, whole genome shotgun sequence.
ACCESSION   DASUPW010000357 DASUPW010000000
VERSION     DASUPW010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776174"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="VFY44_08050"
     CDS             complement(<1..920)
                     /locus_tag="VFY44_08050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5910431.1"
                     /translation="MREGEMGNSTVTTGTGIGVDDFAAVYDRHRSRVYAQCLRRLRSP
                     ADADDATQATFMRAFTAMNRGSGPRRLGPWLATIARNECCDRLSAAGRAVPMDMNDLA
                     LADDGPAPDAGVLAEAEGAEVRAALEQLPFAQRSVLHLREVRELSYAEVAERLAMPVT
                     AVETLLFRARNNLREEVLAVRSPVKCHRARQLGEAVRDGDAITSLERKMLRNHRSECA
                     LCSWLPVRRARAARAAAILVATAAAASARRLIVAPVADALVSAGAPAKTAAVAVAAGS
                     VAAGGLAVQGPAPHAPERRAAQVREAAPAKA"
     gene            1034..1684
                     /locus_tag="VFY44_08055"
     CDS             1034..1684
                     /locus_tag="VFY44_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910432.1"
                     /translation="MIAAALSAVAVAPAAAHGPGGGGPGRGGDDQLLDSDLTLAGTLG
                     VTSAGKSRTTTVEDRDGNSKTITIKRAVKASGDVTADIKTAAGGDPGALTGAIKFSTR
                     SSSTRSKLDGILTVSSTGGKLRLRVHGRAGGDGVYDVRIGKARGTGDLAGAKVSAEGD
                     LTVTDSAVTLDLDGEYGYPSADEAEGGQAGRGRGGRDCPPRGGQQDEQQPGGSSRR"
     gene            complement(1740..2552)
                     /gene="gluQRS"
                     /locus_tag="VFY44_08060"
     CDS             complement(1740..2552)
                     /gene="gluQRS"
                     /locus_tag="VFY44_08060"
                     /inference="COORDINATES: protein motif:HMM:NF004314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEX5910433.1"
                     /translation="MHTGRFAPSPSGPLHAGNLRTALVAWLRARSLGASFLLRIEDLD
                     PQRSRRAHEDGQLADLRAVGIDWDGEPVRQSERHDLYADALAELDAQGRVYPCWCTRA
                     EIREASQAPHGPLPEGAYPGTCRRLSAAERAEKERTADRPPALRLDARAETVAFTDLV
                     HGEVESAVDDITLRRWDGAYAYNLAVIVDDAEQSIGEVVRGDDLLDTTPRQLLIARLL
                     GLTAPRYGHVPLMLDADGKRLAKRDGPAPSGQDLATLRADAEAILGRFIRGS"
     gene            complement(2650..3720)
                     /locus_tag="VFY44_08065"
     CDS             complement(2650..3720)
                     /locus_tag="VFY44_08065"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEX5910434.1"
                     /translation="MPGYSALELLPAEPRDLTDPEVWVRSGWRSRARREAAAHKSEMV
                     APSPRGLSLAALLAVTGVPAAGVISGALGGSGGTEAQAKTAVASRGGGVSALQRAIGV
                     SADGVFGPQTQKALKAYQRNHGLTADGIAGPQTRAKAGLGPGPVLKRGGKARASGVRA
                     TRSVAARGGGVQALQRAIGVSADGVFGPQTEAALKRWQGSHGLEADGIAGPMTRAKAG
                     LGPGPVLKRKSARSAGGGGGDSRVAGVIAAANRIATTPYKYGGGHGSFNDSGYDCSGS
                     VSYALRGGGLLKTPLASGGFTSWGAPGPGKRITVYANAGHAYMVIDGRRFDTSARSQT
                     GNRWTSESRSSAGYVARHPVGL"
     regulatory      complement(3833..3989)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(4083..5129)
                     /locus_tag="VFY44_08070"
     CDS             complement(4083..5129)
                     /locus_tag="VFY44_08070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX5910435.1"
                     /translation="MAHDLKLGFNTGYWSAGPPDGAPEAVAEADKLGYDSIWTAEAYG
                     SDVLTPLAWWGAATQNVRLGTAIMQMSARTPAASAMAAMTMDHLSGGRFILGLGVSGP
                     QVVEGWYGQPFAKPLARTREYVAIVRDIMARGNRVTADGPHYKLPLEGGTGLGKPLKS
                     TLHPVREQMPIFLAAEGPKNVALAGEICDGWLALFYSPHHDGFYREALTEGLGRDGAR
                     RDADSFEVCATVPLIVHDDVEEAADWLRPMYALYFGGMGAKGANFHNDVPRRMGYEDE
                     AERIQDLYLDGKKDEACAAVPTELIEQLALIGPEDKIRSELPAWRESLVTTIMVGGGV
                     QQVRQAADLVLGAQ"
BASE COUNT          665 a         1989 c         1955 g          699 t
ORIGIN      
        1 gccttcgccg gcgccgcctc gcgcacctgt gcggcgcggc gctcgggcgc gtgcggggcc
       61 ggcccctgca ccgccaggcc gcccgccgcg acgctgcctg cggccacggc cacggcggcc
      121 gtcttggcgg gagcgccggc cgacaccagc gcgtcggcca ccggggccac gatcagcctc
      181 cgtgcgctcg cggcggctgc ggtggccacc aggatcgccg ccgcgcgggc cgcgcgcgcc
      241 cggcgcacgg ggagccacga gcacagggcg cactccgagc ggtggttgcg cagcatcttg
      301 cgctccagcg aggtgatcgc gtcgccgtcg cgcacggcct cgcccagctg gcgggcgcgg
      361 tggcacttca ccggcgagcg cacggccagg acctcctcgc ggaggttgtt gcgggcgcgg
      421 aacagcagcg tctcgacggc ggtcaccggc atggccagcc gttccgccac ctcggcgtag
      481 gacagctcgc ggacctcgcg caggtgcagc accgagcgct gggcgaacgg aagttgctcc
      541 agcgcggcgc ggacctcggc gccctcggcc tcggccagca cgccggcgtc gggcgcgggg
      601 ccgtcgtcgg ccagcgccag gtcgttcatg tccatgggca ctgccctgcc ggcggccgac
      661 agccggtcgc agcactcgtt gcgggcgatc gtggccagcc acgggcccag ccggcgcggg
      721 ccgctgccgc ggttcatcgc cgtgaacgcg cgcatgaagg tcgcctgcgt ggcgtcgtcg
      781 gcgtcggcgg gggagcgcaa tcggcgtagg cactgcgcgt acacgcggct ccggtggcgg
      841 tcgtacacgg cggcgaagtc gtcgactccg atgccggtgc cggtggtcac ggtcgagttg
      901 cccatctctc cctctctcat cgctgaccac ctgacgccct cggcgtggcg aaccttgcgc
      961 tcttagaccg caaggtttcg ccgtggcccc tcgtcttgcc ggcatgaacc gatctcgaat
     1021 cacacacact gcgttgatcg cggcggcgct gagcgccgtc gccgttgcac ccgccgcggc
     1081 ccacggcccg ggcggcggcg ggcccggacg aggcggtgac gaccagctgc tggactccga
     1141 cctgacgctg gccggcacgc tcggcgtgac ctccgcgggc aagtcccgca cgaccacggt
     1201 cgaggaccgt gacggcaaca gcaagacgat cacgatcaag cgcgccgtca aggcgtcggg
     1261 cgacgtgacc gccgacatca agaccgccgc cggcggcgac ccgggcgcgc tgaccggggc
     1321 gatcaagttc agcacccgct cctccagcac ccgcagcaag ctcgacggca tcctgaccgt
     1381 cagctccacg ggcgggaagc tgcgcctgcg cgtgcacggc cgcgccggcg gcgacggcgt
     1441 ctacgacgtg cgcatcggca aggcccgcgg caccggggac ctggccgggg ccaaggtctc
     1501 ggccgagggc gacctgacgg tcaccgactc cgccgtcacg ctggacctgg acggcgagta
     1561 cgggtacccg tcggccgacg aggccgaggg cggccaggcc ggccggggcc gcggtggccg
     1621 tgactgcccg ccgcgcgggg ggcagcagga cgagcagcag ccgggcggat cgtcgcgccg
     1681 ctgagcacgg cagctgggag ggctcggcca ccatcggtgg tcgagcctcc gcccgctgtc
     1741 taagaccctc gtatgaagcg gccgaggatg gcctcggcgt cggcgcgcag ggtcgccaga
     1801 tcctggcccg agggagcggg cccgtcgcgc ttggccagcc gcttgccgtc ggcgtccagc
     1861 atcagcggca cgtgcccgta gcgcggtgcc gtcagcccca gcagccgggc gatcagcagc
     1921 tggcgcggcg tggtgtccag caggtcgtca ccgcggacga cctcgccgat cgactgctcg
     1981 gcgtcgtcca cgatcacggc caggttgtac gcgtacgccc cgtcccagcg gcgcagcgtg
     2041 atgtcgtcga cggcggactc cacctcgccg tgcaccagat cggtgaacgc gaccgtctcg
     2101 gcgcgggcgt ccaggcgcag cgcgggcggg cggtcggcgg tgcgctcctt ctcggcccgc
     2161 tcggccgcgc tcagccggcg gcaggtgccg gggtaggcgc cctcgggcaa cgggccgtgc
     2221 ggtgcctgcg aggcctcccg gatctcggcc cgcgtgcacc agcacgggta cacccggccc
     2281 tgcgcgtcca gctccgccag cgcgtcggcg tacaggtcgt ggcgctcgga ctggcggacg
     2341 ggctccccgt cccagtcgat ccccaccgcg cgcaggtcgg ccagttgccc gtcctcgtgc
     2401 gcgcgccggg accgctgcgg atcaaggtcc tcgatcctca gcaggaacga cgcgcccagc
     2461 gaccgggcac gcagccaggc caccagcgcg gtgcgcaggt tgcccgcgtg cagcgggccc
     2521 gaggggctgg gggcgaagcg gccggtgtgc acgcgttcag cgtgtcagga gcgcggcgca
     2581 gccgcgcggt tacggcgccc ggaaggcgca gtgcccatca gagcgcgggc atggatgccc
     2641 gcaagagcac tacagcccga ccgggtgccg cgccacgtag cccgcgctcg agcgcgactc
     2701 cgacgtccag cggttgccgg tctggctgcg cgcgctggtg tcgaaccggc gcccgtcgat
     2761 gaccatgtag gcgtggcccg cgttcgcgta gaccgtgatc cgcttgcccg gcccgggggc
     2821 gccccagctg gtgaagccgc ccgaggccag cggcgtcttc agcaggccac cgccgcgcag
     2881 ggcgtaggac accgagcccg agcagtcgta gcccgagtcg ttgaacgagc cgtggccgcc
     2941 gccgtacttg tagggcgtgg tcgcgatccg gttcgccgcg gcgatcacgc ccgccacgcg
     3001 cgagtcgccg ccaccgccgc cggcgctgcg ggcgctcttg cgcttgagca ccgggccggg
     3061 gccgagcccg gccttggcgc gggtcatcgg gccggcgatg ccgtcggcct ccagcccgtg
     3121 cgatccctgc cagcgcttga gcgcggcctc ggtctggggg ccgaacacgc cgtcggcgga
     3181 cacgccgatc gcgcgctgca gcgcctggac gccgccacca cgggcggcca ccgatcgggt
     3241 ggcgcgcacg ccggaggcgc gcgccttgcc gccgcgcttg agcaccgggc cggggccgag
     3301 gccggccttg gcgcgggtct gcgggccggc gatgccgtcg gccgtcagcc cgtgattgcg
     3361 ctggtacgcc ttcagggcct tctgcgtctg ggggccgaac accccgtcgg cggacacgcc
     3421 gatggcgcgc tgcagggcgc tgacgccgcc gccgcgcgag gcgaccgccg tcttggcctg
     3481 cgcctcggtg ccgccgctgc cgcccagggc gcccgagatc acgccggcgg ccggcacgcc
     3541 ggtcaccgcc agcagtgcgg ccagcgagag cccgcgcggg gagggggcga ccatctcgct
     3601 cttgtgcgcg gccgcctcgc ggcgggcacg tgagcgccaa cccgagcgca cccacacctc
     3661 ggggtcggtc aggtcgcgcg gctccgccgg cagcagctcg agcgccgagt atccgggcac
     3721 gagtcctgca cggcgcctac gcgcctggga gcgttccagg gattcggacc agagatcgcg
     3781 tgacgtcagg tccgcgggga cggaattcaa gatgcattta cctcgctgtc ggtacttcat
     3841 gtcgcctacg aggtgagctg acgggctcgc gccgtacaga cggcgctacg ctcggtgccg
     3901 gctgagccgg cgcctggacg attcgccccc gcggtacgtg gaccgcacgg ttcccccgtt
     3961 cgtccaggac gggccaggac gattcggcag cgggcgtcgt tgtatcaggc cgaattcctt
     4021 ccgcgtgcag ggattcttgc agcgcgcagg gtgcacgaaa ccgcccaggg ttatggcaac
     4081 cgttattggg cgcccagcac cagatccgcg gcctggcgaa cctgttgcac gccgccgccg
     4141 accatgatcg tggtcaccag cgattcccgc caggcaggca gctcggagcg gatcttgtcc
     4201 tccgggccga tcagcgcgag ctgctcgatc agctcggtgg gcaccgccgc gcaggcctcg
     4261 tccttcttgc cgtcgaggta gaggtcctgg atcctctccg cctcgtcctc gtagcccatc
     4321 cggcggggga cgtcgttgtg gaagttcgcg cccttggcgc ccatgccgcc gaagtacagc
     4381 gcgtacatgg ggcggagcca gtcggccgcc tcctccacgt cgtcgtgcac gatcagcggg
     4441 accgtggcgc agacctcgaa cgagtccgcg tcgcggcggg cgccgtcgcg gcccaggccc
     4501 tcggtcagcg cctcgcggta gaagccgtcg tggtgggggc tgtagaacag ggccagccag
     4561 ccgtcgcaga tctcgcccgc gagggccacg ttcttcggcc cctcggccgc caggaagatc
     4621 ggcatctgct cccgcacggg gtgcagggtg ctcttcagcg gcttgcccag cccggtcccg
     4681 ccctccagcg gcagcttgta gtgggggccg tcggccgtca cccggttccc gcgggccatg
     4741 atgtcgcgca cgatcgccac gtactcgcgc gtgcgcgcca gcggcttggc gaacggctgg
     4801 ccgtaccagc cctcgaccac ctgcggcccc gacacgccca ggcccaggat gaagcgcccg
     4861 ccggagaggt ggtccatggt catggccgcc atcgcggacg cggccggcgt gcgcgccgac
     4921 atctgcatga tcgccgtgcc caggcgcacg ttctgggtgg ccgcgcccca ccaggccagc
     4981 ggcgtcagca cgtccgagcc gtaggcctcc gcggtccaga tcgagtcgta gccgagcttg
     5041 tcggcctcgg ccaccgcctc cggcgcgccg tcgggcgggc cggcgctcca gtagccggtg
     5101 ttgaagccga gcttgaggtc gtgggccatc ggccgaccct atctcgacgc cggcgttctc
     5161 gccgccggcc gcaccagtcg ctcggcccac ggctgggccg ggcagggcgc ggggctcggt
     5221 tcgaagccgt cgccgaggga acagatctcg gcccgcgccc ctactccgtc acgcagccgt
     5281 gctcgtccag cccgaacgtc agctcggg
//
LOCUS       DASUPW010000358         2184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1885273, whole genome shotgun sequence.
ACCESSION   DASUPW010000358 DASUPW010000000
VERSION     DASUPW010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1885273"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VFY44_08075"
     CDS             complement(<1..797)
                     /locus_tag="VFY44_08075"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5910436.1"
                     /translation="MKVFVTGATGFIGGRMTHLLAGRGDEVTALVRDPAKADDLAAAG
                     VRLIQGDLTSLEAIEEGVRGADAVVHGAAVYKVGIPAAERDDMFDANVRGTARVLDAA
                     IEANVPRIVYVSSVVAFGDTEGATVTEDHVRPHRRFSSFYEETKVLAHEIADERITRG
                     APIVMVQPGGVYGPGDHSQLGTMIHQAATGKLKMKMLPEAGYLLAYVDDVAAGILGAL
                     DAGEIGESYVLGGECVTMGELVDRAAAAAGRKGPRATMPTPLIKASAP"
     gene            827..2107
                     /gene="hflX"
                     /locus_tag="VFY44_08080"
     CDS             827..2107
                     /gene="hflX"
                     /locus_tag="VFY44_08080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX5910437.1"
                     /translation="MAQSYGIRNRRARQRALLISAGPDEHDLSELGELLRTAGVAVAG
                     EMTQKRATPDPDRYFGKGKLAELKQAIKDNDANLVAVDDELAPRQERNLEAAIDTPVI
                     DRTAVILDIFADHAGSAEGKLQVELAQLEYNMARMRGLWTHLERLGGSTGGGIATRGP
                     GETQIETDRRLARSRIAALRRRLERTRGVRQTMRNERERAGLPTVALAGYTNAGKSTL
                     LNALTGAEVGVRDRLFHTLDPTTRQMEISGRTYLLTDTVGFIRKLPHQLVEAFGATLE
                     EAMGADLLLHVVDASAPEEELHEMLSAVEDVLAEIGAGEVPTVLALNKADLLDAERRR
                     ELSFRFPKAVQVSAATGEGLDDLREAVEARFLATLRPMELLVPYGEGGSLSELHDVAG
                     EVEREDTPEGVKVRARVPAVAAARFERFALNGRH"
BASE COUNT          314 a          815 c          779 g          276 t
ORIGIN      
        1 ggtgcggagg ccttgatcag gggcgtcggc atggtcgcgc gcggcccctt gcggcccgcg
       61 gcggcggcgg cgcggtcgac cagctcgccc atggtcacgc actcgccgcc cagtacgtag
      121 gactcaccga tctcgccggc gtccagggcc ccgaggatgc ccgcggccac gtcgtccacg
      181 tacgccagca ggtagccggc ctcgggcagc atcttcatct tcagctttcc cgtggcggcc
      241 tggtggatca tcgtgcccag ctgggagtgg tccccggggc cgtagacccc gccgggctgg
      301 accatcacga tcggcgcgcc gcgggtgatg cgctcgtcgg cgatctcgtg cgccagcacc
      361 ttggtctcct cgtagaacga cgagaagcgg cggtgcgggc gcacgtggtc ctcggtcacg
      421 gtggcgccct cggtgtcgcc gaaggccacc accgacgaca cgtagacgat gcgggggacg
      481 ttggcctcga tggccgcgtc cagcacgcgc gcggtgccgc gcacgttggc gtcgaacatg
      541 tcgtcgcgct ccgcggccgg gatgccgacc ttgtagaccg ccgcgccgtg gaccacggcg
      601 tcggcgccgc ggacgccctc ctcgatcgcc tcgagcgacg tcaggtcgcc ctggatcagc
      661 cggacgccgg cggcggccag gtcgtccgcc ttggcggggt cgcgcaccag cgccgtcacc
      721 tcgtcgccgc gccccgccag caggtgggtc atgcgacccc cgatgaagcc cgtggctccc
      781 gtcacgaaca ccttcatggc ctgcatcgta cgtacactca aacgtgatgg cgcagagcta
      841 cggaatccgc aaccgcagag cgcgccagcg cgcgctgctg atctccgccg ggccggacga
      901 gcacgacctg tccgagcttg gagagctcct gcgcacagcc ggcgtcgccg tggccggcga
      961 gatgacccag aagcgcgcca cgcccgaccc cgaccgctac ttcggcaagg gcaagctggc
     1021 cgagctcaag caggccatca aggacaacga cgccaacctg gtcgcggtcg acgacgagct
     1081 ggcgccgcgg caggagcgca acctcgaggc ggccatcgac acgccggtga tcgaccgcac
     1141 cgcggtgatc ctggacatct tcgccgacca cgccggatcg gccgagggca agctgcaggt
     1201 cgagctcgcc cagctcgagt acaacatggc ccgcatgagg gggctgtgga cccacctcga
     1261 gcgcctcggg ggctccaccg gcggcggcat cgccaccagg ggcccgggcg agacccagat
     1321 cgagaccgac cgccgcctgg cccggtcgcg catcgccgcc ctgaggcggc ggctggagcg
     1381 cacgcgcggc gtgcgccaga ccatgcgcaa cgagcgcgag cgcgcgggcc tgcccacggt
     1441 ggccctggcg ggctacacca acgccggcaa gtcgacgctg ctgaacgccc tgaccggggc
     1501 cgaggtaggg gtcagggacc ggctgttcca cacgctcgac ccgacgaccc gccagatgga
     1561 gatctcgggc cgcacctacc tgctgaccga caccgtcggc ttcatccgca agctgccgca
     1621 ccagctggtc gaggccttcg gcgccacgct cgaggaggcc atgggcgccg acctgctgct
     1681 gcacgtggtc gacgcgtccg cgcccgagga ggagctgcac gagatgctct ccgccgtcga
     1741 ggacgtgctg gccgagatcg gggccggcga ggtgcccacc gtcctggcgc tgaacaaggc
     1801 cgacctgctg gacgccgagc gccgccgcga gctgtcgttc cgcttcccca aggcggtcca
     1861 ggtgtccgcg gccaccggcg aggggctgga cgacctgcgc gaggccgtcg aggcccgatt
     1921 cctggccacg ctgcgcccga tggagctgct ggtgccctac ggcgagggcg gctcgctgtc
     1981 cgagctgcac gacgtggccg gcgaggtgga gcgcgaggac acacccgagg gcgtgaaggt
     2041 gcgcgcccgg gtgcccgcgg tcgccgccgc ccggttcgag cgcttcgcgc tcaacggccg
     2101 ccactgatgg agctgcgctt ccggcggctc gacgccgccg ccacgccgcc cgcgcgcgcc
     2161 cacgacgacg acgccgggtt cgac
//
LOCUS       DASUPW010000359         3501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1192983, whole genome shotgun sequence.
ACCESSION   DASUPW010000359 DASUPW010000000
VERSION     DASUPW010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192983"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VFY44_08085"
     CDS             <1..608
                     /locus_tag="VFY44_08085"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5910438.1"
                     /translation="DVQRLIDASSAVDILIANAALPASGRIEDFSVEQIDRALDVNLR
                     APIHLARELAGPRVERGSGHLVFISSLSGKTASPGGSLYSATKFGMRGYALGLREDLV
                     GTGVGVTTVFPGFIRDAGMFHEADTELPKGVGTRTPREVADGVLKGIDRDKPEVAVAP
                     LALRLGSIAGGVAPGPMNALQRKMGSRDIAAQMAKGQANKR"
     regulatory      670..813
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            809..1378
                     /locus_tag="VFY44_08090"
     CDS             809..1378
                     /locus_tag="VFY44_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910439.1"
                     /translation="MTQRFPRHTLLCALAVLSLFAVLGAGSASAAPTGGAQFVPPPPP
                     PKKAKLLKSGRVLSPAGAPAKVRRVIAAANQIVNKPYRWGGGHGAFTSRLAAGYDCSG
                     AVSHALRGGRFLKTPMASGGLMSWGRRGTGRWITVYAHGGHAYVVVAGLRFDTSMRDA
                     NAPGPSTGPRWSKTLRRSAAFVARHPKNF"
     gene            1404..1712
                     /locus_tag="VFY44_08095"
     CDS             1404..1712
                     /locus_tag="VFY44_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910440.1"
                     /translation="MSLAREVTYLRTLGLGDVDLSRATGSADQARSWPPSDPAESGDW
                     TARIGELVAIAERLEYLIEREKIAPWLRTSLPEAGWHTPLDLIGQGRAEQVVEVIEAG
                     "
     gene            complement(1709..>3501)
                     /locus_tag="VFY44_08100"
     CDS             complement(1709..>3501)
                     /locus_tag="VFY44_08100"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX5910441.1"
                     /translation="PVLIHGDASFPGQGIVAETLNLQALAGYSTGGTIHIIANNQLGF
                     TTDPNDSRSTRYASDLAKGFDLPIIHVNADDVEACIAAVRLAVAFRNEFGRDVLIDLV
                     GYRRFGHNETDEPAYTQPQMYEAIKAHPPVREVYAKQLEEQGVVSAADAESMAAECYE
                     RVAAAHTELKETMAGPPDTGRHEIDTTMSREPKTTLPEDTLRSLNEQLLRVPDGFNVH
                     RKLKPFLQRRRDAAEEGGRVDWAHAESLAFASLLALGVPVRMTGQDTERGTFSQRHLV
                     FHDAENGDRFTPLQNISSAVAPFELHNSPLSEQACVGFEYGYSAADPEALVLWEAQFG
                     DFVNSAQVILDQFLVSGLAKWGATSRLTLLLPHGYEGSGPEHSSGRVERFLQACAEGN
                     MRVANTTTPAQYFHLLRRQALVSKARPLVLMTPKSLLRLPAATSSLEDLYEGGFKRVL
                     DDPAAPEDRSKVTKLVLCSGKVYYDIAANEDRAQQEHIAVARVEMLYPFPDEELRELM
                     SSYPNLERVVWVQEEPRNMGPRAYMRRRMAAILPEDLHYFYVGRQLRAAQSEGYTAAH
                     RKEQARIVRVALDLEDDVLERDASAERPRL"
BASE COUNT          483 a         1221 c         1284 g          513 t
ORIGIN      
        1 cggatgtgca gcggctgatc gacgcctcat cggcggtgga catcctcatc gccaacgccg
       61 cgctgccggc gagcggacgg atcgaggact tcagcgtcga gcagatcgac cgcgcgctcg
      121 acgtgaacct gcgggcgccg atccacctgg cccgcgagct ggcgggcccg agggtggagc
      181 gcggctcggg ccacctggtg ttcatctcgt cgctgtcggg caagacggcc agcccgggcg
      241 gctcgctgta ctcggccacc aagttcggca tgcgcgggta cgcgctgggg ctgcgcgagg
      301 acctggtggg cacgggcgtg ggcgtgacga ctgtcttccc cggcttcatc cgcgacgccg
      361 gcatgttcca cgaggccgac accgagctgc ccaagggcgt cggcacccgg acgccccgag
      421 aggtggccga cggcgtgctc aagggcatcg accgcgacaa gcccgaggtg gcggtggcgc
      481 ccctggcgct gcggctgggg tcgatcgccg gcggggtggc cccggggccg atgaacgccc
      541 tgcagcgcaa gatgggcagc cgcgacatcg cggcgcagat ggccaagggc caggcgaaca
      601 aacgctgacc gctctgtgac ccacctcaca taaccacgcg caacctttcg cccaaaccgg
      661 tgttctaggc ccccaacaac gccgaatccg cgtatccacg cggagcgggg gacccaatca
      721 gtccccgggg ccaatcggga aatccttccc gacgcgcaag ctctttcagc gcgcgcccga
      781 cagctaaccc cgcaggcgga cgagagagat gacccaacgt ttcccccgac atacgctgct
      841 gtgcgcgctt gccgtgctct ccctgttcgc cgtgctgggc gcgggctccg cgtcggcggc
      901 cccgaccggc ggggcgcagt tcgtgccgcc cccgccgccg cccaagaagg ccaagctgct
      961 caagagcggc cgggtcctgt cacccgcggg cgcgccggcg aaggtcaggc gcgtgatcgc
     1021 ggcggcgaac cagatcgtga acaagcccta ccgctggggc ggtgggcacg gcgccttcac
     1081 cagccgcctg gccgcgggat acgactgcag cggtgccgtc agccatgcgc tccgcggcgg
     1141 gcgcttcctg aagacgccga tggcgtcggg cggcctgatg agctggggcc gtcgcggcac
     1201 cggccgctgg atcacggtgt acgcccacgg cggccacgcc tacgtggtcg tggccggcct
     1261 gcgcttcgac acctcgatgc gtgacgccaa cgcccccggg ccgagcaccg gcccgcgctg
     1321 gagcaagacg ctgcgccgct cggccgcctt cgtggcgcgc cacccgaaga acttctagac
     1381 cccgtcgggg tcacaatggc tcgatgtccc tcgcccgcga ggtcacctac ctgcgcaccc
     1441 tcggcctggg ggacgtggac ctgtcgcggg ccacgggctc tgccgaccag gcccgcagct
     1501 ggccgccgtc cgacccggcc gagtccgggg actggacggc tcggatcggt gagctggtgg
     1561 ccatcgccga gcgcctggag tacctgatcg agcgcgagaa gatcgccccc tggttgcgca
     1621 cgtcgctgcc cgaggccggt tggcacacgc cgctggacct gatcggccag ggccgcgccg
     1681 agcaggtggt cgaggtgatc gaggccggct agagacgcgg gcgctcggcg gaggcgtcgc
     1741 gctcgagcac gtcgtcctcc aggtccagcg ccacccggac gatccgcgcc tgctccttgc
     1801 ggtgcgcggc ggtgtagccc tcgctctgcg cggcgcgcag ctggcggccg acgtagaagt
     1861 agtgaaggtc ctcgggcagg atggccgcca tgcgccggcg catataggcg cgcgggccca
     1921 tgttgcgcgg ctcctcctgg acccacacca cgcgctcgag gttggggtag ctggacatca
     1981 gctcgcggag ctcttcgtcg gggaaggggt agagcatctc cacccgggcc acggcgatgt
     2041 gctcctgctg ggcgcggtcc tcgttggcgg cgatgtcgta gtagaccttg cccgagcaca
     2101 ggacgagctt cgtgaccttg gagcggtcct ccggcgcggc cgggtcgtcc agcacgcgct
     2161 tgaagccgcc ctcgtacagg tcctcgagcg aggaggtggc ggcgggcagg cgcagcaggc
     2221 tcttgggcgt catcagcacc agcggccgcg ccttggacac cagcgcctgg cggcgcagca
     2281 ggtggaagta ctgcgccggc gtggtggtgt tggccacgcg catgttgccc tcggcgcacg
     2341 cctgcaggaa gcgctcgacg cggcccgagg agtgctcggg gcccgagccc tcgtagccgt
     2401 gcggcagcag cagcgtcagg cgcgaggtcg cgccccactt ggccaggccc gagaccagga
     2461 actggtccag gatcacctgg gccgagttca cgaagtcacc gaactgcgcc tcccacagca
     2521 ccagcgcctc cgggtcggcg gccgagtagc cgtactcgaa cccgacgcag gcctgctcgg
     2581 acagcgggct gttgtgcagc tcgaacgggg ccacggcgct gctgatgttc tgcagcggcg
     2641 tgaagcggtc gccgttctcg gcgtcgtgga agaccaggtg gcgctggctg aaggtgccgc
     2701 gctcggtgtc ctggccggtc atgcgcaccg gcacgcccag ggccagcagc gacgcgaagg
     2761 ccagcgactc ggcgtgcgcc cagtccacgc gcccgccctc ctcggccgcg tcccgccggc
     2821 gctggaggaa gggcttgagc ttgcggtgca cgttgaagcc gtcgggcacg cgcagcagct
     2881 gctcgttgag cgagcgcagc gtgtcctcgg ggagcgtggt cttgggctcc cggctcatgg
     2941 tggtgtcgat ctcgtggcgg cccgtgtcgg gcgggcccgc catggtctcc ttgagctcgg
     3001 tgtgggcggc ggccacgcgc tcgtagcact ctgcggccat gctctcggcg tccgccgcgc
     3061 tgaccacgcc ctgctcctcg agctgcttgg cgtacacctc gcggaccggg gggtgggcct
     3121 tgatggcctc gtacatctgc ggctgggtgt acgcgggctc gtcggtctcg ttgtggccga
     3181 agcgccggta gccgaccagg tcgatcagca cgtcgcggcc gaactcgttg cggaaggcga
     3241 ccgccaggcg caccgcggcg atgcaggcct ccacgtcgtc ggcgttcaca tggatgatcg
     3301 gcaggtcgaa ccccttggcc aggtccgacg cgtagcgcgt ggagcgcgag tcgttggggt
     3361 cggtggtgaa gcccagctgg ttgttggcga tgatgtggat cgtgccgccg gtgctgtagc
     3421 cggccagcgc ctggaggttc agcgtctcgg ccacgatgcc ctgtccgggg aacgaggcgt
     3481 cgccgtggat caggaccggc a
//
LOCUS       DASUPW010000360         4065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1469564, whole genome shotgun sequence.
ACCESSION   DASUPW010000360 DASUPW010000000
VERSION     DASUPW010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469564"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="VFY44_08105"
     CDS             complement(<1..856)
                     /locus_tag="VFY44_08105"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX5910442.1"
                     /translation="MRERVIGAVVAVAACLAAATGAGAQTPAPLTGPAGAGHARVLVI
                     GVDGARWDLVRRAMAEGRAPNLARLAREGLAGPTLLPYTPPAALTISEVGWSTIASGA
                     GPVKHGVNGFMLNNDPGQATKNGYADFLTRAEALRPATSTFLASDWANIGLARNGGPI
                     FGTAIDARYALAAEDTVDSYNRSDAELTEQAARYLRRGDPDAGFVYLGAVDETAHLIG
                     SATPAYRDAIGAADKRIGALVDAIRRRPTYGTERWTVIVTTDHGQQDLNYPSTLSHGG
                     PSELERTSF"
     gene            925..1251
                     /locus_tag="VFY44_08110"
     CDS             925..1251
                     /locus_tag="VFY44_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910443.1"
                     /translation="MPVADSLTLRYALHELPPGRLPFRRWRWELWHGARLEGTGWRLS
                     ERDASRAVQRHGALVGQRLLGLGSAPPPAPRELEPFRPGTTVHVRGGGVFFSLVPLHL
                     EEPDLS"
     gene            complement(1244..1426)
                     /locus_tag="VFY44_08115"
     CDS             complement(1244..1426)
                     /locus_tag="VFY44_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910444.1"
                     /translation="MREAVRHPAPPRTRPTRVAVDRTAARSRWIEAFYIAEAKAQLDH
                     QSPGRHGGRVPGRPLS"
     gene            complement(1419..2258)
                     /locus_tag="VFY44_08120"
     CDS             complement(1419..2258)
                     /locus_tag="VFY44_08120"
                     /inference="COORDINATES: protein motif:HMM:NF006114.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5910445.1"
                     /translation="MSKVWFITGSSKGFGRIWAEAALERGDKVALTARNVDTVSELAE
                     RYGRENALPLALDVTDKGAVDAAVTQAHEHFGRLDVVVNNAGYGLFGSVEEVSEQQAR
                     DQIETNLFGALWVTQAALPFLREQGSGHIVQVSSIGGVHAFPGIGLYHASKWGLEGFT
                     QALAGEVADLGIDVTIVEPGGFSTDWAGPSSVSADEIAAYQPVRDANAERFSDPSAVG
                     DPEATGPAILELVDAEQPPLRVFFGRKPIDLIRPEYEQRLAEWERWDDLSKRAHGEGA
                     VRA"
     gene            2353..2922
                     /locus_tag="VFY44_08125"
     CDS             2353..2922
                     /locus_tag="VFY44_08125"
                     /inference="COORDINATES: protein motif:HMM:NF044515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5910446.1"
                     /translation="MEATTKTMRADARRNREKLLSSALHLFTEQGADVSLDAVAKHAG
                     VGVGTLYRHFPTREALVEAAYLTELDRLHDGAAELLAEHPPDQALAEWMDLFVEYAQT
                     KRGMAGALQGVIASGGDPYRQSRTKILDAVTTLFDAGQAAGALRDDVDAEDVWRGMAG
                     IFAMPDGPDWPQRSRTQLKLLMDGLRHRA"
     gene            3024..3524
                     /locus_tag="VFY44_08130"
     CDS             3024..3524
                     /locus_tag="VFY44_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910447.1"
                     /translation="MSATPALSMLLLCAALALPGCGGEGDDDPGQKELLGDATFQQAV
                     RAVGVSPATRPVSGLGRAIGVRMRDLTAAERRLAAGSLSRQRFRRRYGQGPLEMCDRL
                     GALVGQLDSRLDPDAELSKAVVAGFGRGGSVKNELSARLPGSESLECAMKYRSSYVFV
                     ARLYRD"
     gene            3536..>4065
                     /locus_tag="VFY44_08135"
     CDS             3536..>4065
                     /locus_tag="VFY44_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910448.1"
                     /translation="MRTLGLQRMPGTVVLEQETLAHVASLPEGGWRGQITARDGNVWV
                     LTPDDRKLTRFDAATVAESGEFRLDRGAVGLLVDERGVLVLHADAQVVRLDPVTGAER
                     QRARLPEGTTELVAGPGVLWGLRGHHPGPEGDQSVSLVQVDPSTLGVQREVELGRSIY
                     AGAPYLAEDKVVALIEV"
BASE COUNT          495 a         1470 c         1515 g          585 t
ORIGIN      
        1 cgaacgacgt gcgctccagc tcgctggggc cgccatggct cagggtgctg gggtagttca
       61 ggtcctgctg gccgtggtcg gtggtcacga tcaccgtcca ccgctcggtg ccgtacgtgg
      121 gccgccggcg gatggcgtcc accagcgcgc cgattcgctt gtcggcggcg ccgatggcgt
      181 cccggtaggc cggggtcgcc gacccgatca ggtgcgccgt ctcgtccacc gcgcccaggt
      241 acacgaagcc cgcgtcgggg tcgccccggc gcaggtaccg ggccgcctgc tcggtcagct
      301 cggcgtcgct gcggttgtag gagtccacgg tgtcctcggc ggccagcgcg tatcgcgcgt
      361 cgatggcggt gccgaagatc ggtccgccgt tgcgggccag gccgatgttg gcccagtcgc
      421 tggccaggaa cgtgctggtc gcgggccgca gcgcttcggc ccgggtcagg aagtcggcgt
      481 agccgttctt ggtggcctgg ccgggatcgt tgttcagcat gaagccgttg accccgtgct
      541 tgaccggccc tgcgccggag gcgatcgtgg accagccgac ctcgctgatg gtcagcgccg
      601 cgggcggggt gtagggcagc agggtcggcc ccgccaggcc ctcccgggcc aggcgggcca
      661 ggttgggggc gcgcccctcg gccatggccc gccgcaccag gtcccagcgt gcgccgtcca
      721 cgccgatcac cagcacgcgg gcgtgaccgg cgcccgccgg cccggtcagc ggggcaggcg
      781 tctgggcgcc ggctccggtg gccgcggcca ggcacgcggc gacagccacc accgcgccga
      841 tcaccctctc ccgcatcggt ccaacgtatc tcgaccgggc gctcgcgcgc gcaggctccg
      901 cggcgatctc tagcctcgga cgccgtgccc gtcgccgact ccctgaccct gcgctacgcg
      961 ctccacgagc tgccgcccgg ccgcctgccg ttccggcgct ggcggtggga gctgtggcac
     1021 ggcgcgcgcc tggagggcac cggctggcgc ctgagcgaac gcgacgcctc gcgcgcggtc
     1081 cagcgccacg gggcgctggt gggccagcgt ctgctggggc tgggctcggc gccgccgccc
     1141 gcgccccggg agctcgagcc gttcaggccc ggcaccacgg tgcacgttcg cggcggcggc
     1201 gtgttcttct ccctggtgcc gctgcacctg gaggagcccg acctcagctg aggggccggc
     1261 cgggaacccg tccgccgtgg cggcccgggg actggtggtc cagctgcgcc ttcgcctcgg
     1321 cgatgtagaa cgcctcgatc cagcgcgatc gtgcggccgt gcggtcgacc gccacgcggg
     1381 tgggacgggt gcgcggcggc gccgggtgtc ggaccgcctc acgcacggac ggccccctcg
     1441 ccgtgcgcgc gcttggacag gtcgtcccag cgttcccact cggccagccg ctgctcgtac
     1501 tcgggcctga tcaggtcgat cggcttgcgg ccgaagaaca cgcgcagcgg cggctgctcg
     1561 gcgtccacca gctccaggat cgccgggccg gtggcctccg ggtcgcccac cgcggacgga
     1621 tctgagaagc gctccgcgtt ggcgtcgcgc acgggctggt aggccgcgat ctcgtcggcg
     1681 ctcaccgacg acgggccggc ccagtcggtg ctgaagccgc cgggctcgac gatcgtgacg
     1741 tcgatcccca ggtcggcgac ctcgccggcc agcgcctggg tgaagccctc gaggccccac
     1801 ttcgaggcgt ggtagaggcc gatgcccggg aacgcgtgca cgccgccgat ggacgagacc
     1861 tggacgatgt ggcccgagcc ctgctcgcgc aggaacggca gcgccgcctg ggtgacccac
     1921 agggcgccga acaggttggt ctcgatctgg tcgcgggcct gctgctcgct gacctcctcg
     1981 accgacccga acaggccgta gccggcgttg ttgaccacca cgtccaggcg gccgaagtgc
     2041 tcgtgcgcct gtgtgacggc agcgtcgacc gcgcccttgt cggtcacgtc cagcgccagg
     2101 ggcagggcgt tctcacggcc gtatcgctcg gccagctcgg acaccgtgtc cacgttgcgg
     2161 gcggtcagcg ccaccttgtc gccgcgctcc agcgcggcct cggcccagat gcggccgaat
     2221 cccttcgagc ttcccgtgat gaaccagacc ttggacattg cgtcctcctg tactcttgac
     2281 catgaagcgg aggagttctc cgtttcctgt agtcaccgta gcagacaacc ggaggatgcc
     2341 tccgtttaca ccatggaagc gaccaccaag accatgcgcg ccgacgcgcg ccgcaaccgc
     2401 gagaagctgc tgagctccgc gctgcacctg ttcaccgagc agggcgcgga cgtgtccctg
     2461 gatgccgtgg ccaagcacgc gggcgtgggg gtgggcacgc tctaccgcca cttccccacg
     2521 cgcgaggcgc tggtggaggc cgcctacctg accgagctgg accgcctcca cgacggcgcc
     2581 gccgagctgc tggccgagca cccgccggac caggcgctgg cggagtggat ggacctgttc
     2641 gtggagtacg cgcagaccaa gcgcgggatg gccggcgcgt tgcagggtgt gatcgcctcc
     2701 ggcggcgacc cctaccgcca gtcgcgcacc aagatcctgg atgccgtgac cacgctgttc
     2761 gacgccggcc aggccgcggg cgcgctgcgc gacgacgtgg acgccgagga cgtctggcgc
     2821 ggcatggccg gcatcttcgc catgcccgac ggccccgact ggccccagcg ctcgcgcacg
     2881 cagctcaagc tgctgatgga cggcctgcgc caccgcgcct gagcaaagtt cagggggtcc
     2941 ccctgacgcg aagcgcacgg gccggtgtca gcttgggttc cgacggacgt cacgtgggac
     3001 agcgaccggt acggtcgctc gtgatgagcg cgacgcccgc cttatcgatg ctgttgctct
     3061 gcgccgccct ggccctgccg ggctgcggag gcgagggcga cgacgacccg ggtcagaagg
     3121 agctgcttgg cgacgcgacc ttccagcagg ccgtgcgcgc cgtcggcgtg tccccggcca
     3181 cccgcccggt gtccggcctg ggccgggcga tcggcgtgcg gatgcgcgat ctgacggcgg
     3241 ccgagaggcg cctggccgcg gggtcgctct cccggcagcg gttccggcgc cggtacggcc
     3301 agggcccgct ggagatgtgc gaccgcctgg gggcgctggt ggggcagctg gactcccggc
     3361 tggacccgga cgccgagctg tcgaaggccg tcgtggccgg cttcggtcgc ggtggctccg
     3421 tcaagaacga gctcagcgcg cggctgcccg gctcggagtc gctggagtgc gcgatgaagt
     3481 accggtcctc gtacgtgttc gtggcgcggc tctaccggga ctgaccatgg gcctcttgcg
     3541 gacactcggc ctgcagcgca tgcccggaac ggtggtgctc gagcaggaga cgctggccca
     3601 cgtcgcctcg ctgccggagg gcggctggcg tgggcagatc acggcccgcg acggcaacgt
     3661 ctgggtgctc acgcccgacg accgcaagct cacccgcttc gacgcggcca cggtggccga
     3721 gagcggcgag ttccggctcg accggggtgc cgtcggcctg ctggtggacg agcgcggcgt
     3781 gctggtgctg cacgccgatg cccaggtggt gcgcctggac ccggtgacgg gcgccgagcg
     3841 ccagcgcgcc cggctgcccg agggcacgac cgagctggtt gccggccccg gggtcctgtg
     3901 gggccttcgc ggccaccatc ccggccccga gggcgaccag tcggtgagcc tcgtgcaggt
     3961 ggacccgtcg acgctgggtg tccagcgcga ggtcgagctg ggccgctcga tctacgcggg
     4021 cgcgccctac ctcgccgagg acaaggtggt cgcgttgatc gaggt
//
LOCUS       DASUPW010000361         2007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_776690, whole genome shotgun sequence.
ACCESSION   DASUPW010000361 DASUPW010000000
VERSION     DASUPW010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776690"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VFY44_08140"
     CDS             complement(<1..765)
                     /locus_tag="VFY44_08140"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5910449.1"
                     /translation="MKKPLILIVFTLAVVWFAIDALRGLSADTPGSRSAAEGARSGQD
                     AALVVGSANFPENALLAELYAGALSAAGLEVDTALNLGSREVLFPAMDNGDITIVPEY
                     TGALLDYLAGAEAAPATSTDAQLAQLEIVLPEGLKLLDPSTAQDQETVTCRPEIVERY
                     QLESLDDLGPVSGALVMGGPPELAQRAGRFSLPGLKSAYGIEFAEFKPLDVAGPLTVA
                     ALRAGKIDCANLFSTQSAITTNGFISLRDPRGLIESQ"
     gene            complement(762..1421)
                     /locus_tag="VFY44_08145"
     CDS             complement(762..1421)
                     /locus_tag="VFY44_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5910450.1"
                     /translation="MRLLEHLWYSIVGIVLGAGIGLPLGLLTGHTGRGSFAAAALSNY
                     WRALPTLGVVVLVFRLAPLSVWPVLAALVAIAVPPILLNTDAGLRSVDEAVRDAAAGM
                     GLTSSQRLWRVEVPVALPLIVTGLRSASAQVIATTSVAAFVGLGGLGRYIIDGYASRD
                     LAQITAGAVLIAATALLVDGCFALAQRRLTSRPRARAPVHFGRLPALTGSTRPRKETS
                     S"
     gene            complement(1486..>2007)
                     /locus_tag="VFY44_08150"
     CDS             complement(1486..>2007)
                     /locus_tag="VFY44_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5910451.1"
                     /translation="GLAAYQWPRLYPPMRAATQILYTVPALAMFVLLVSLVGLGPLPV
                     VFALASYSLVILVANLVEGLRAVPRDVVQAATAMGYRQWARVVRVELPLATPALLAGL
                     RAATVSTISLVSLGAVIGTGGLGQLFIHGFQIDNPIEIWAGLVLTVALALVADLLLLA
                     AAAGIAPWTRAAR"
BASE COUNT          352 a          649 c          740 g          266 t
ORIGIN      
        1 ttgggactcg atcaggcctc gcgggtctct gagtgagatg aacccgttgg ttgtgatcgc
       61 ggactgggtg ctgaagaggt tggcgcagtc gatcttcccg gctctcagcg cggcgacggt
      121 caggggcccg gccacgtcga gaggcttgaa ttcggcgaac tcgatgccgt acgccgactt
      181 gagtcccgga aggctgaacc ggccggcgcg ttgggcgagc tcgggcgggc cacccatgac
      241 gagtgccccg ctgaccgggc cgaggtcatc gaggctttcc agctggtacc gctcaacgat
      301 ctcggggcgg cacgtgacgg tttcctggtc ctgggcggtg gacgggtcga gcaacttcaa
      361 cccctccgga agcacgatct ccagctgcgc gagctgcgcg tcggtgctgg tcgcgggagc
      421 ggcctcagcc cccgcgaggt agtccaacag cgcaccggtg tactcgggca cgatggtgat
      481 gtcgccgttg tccatggccg ggaagagcac ctcacgcgag ccgaggttga gggcagtgtc
      541 gacctcgaga cccgcggcgg agagggcgcc ggcgtacagc tcggcgagca gcgcgttctc
      601 ggggaagttg gccgagccta cgacaagcgc agcgtcctgc ccgctacgtg cgccttcggc
      661 agccgaacgg gagcccggtg tgtcagcgct gaggccgcga agtgcgtcga tcgcaaacca
      721 caccacggcg agggtgaaga cgatgagaat caggggcttc ttcatgacga tgtctccttg
      781 cgaggtcgag tcgaaccggt cagggcaggc agacgtccga agtgcacggg tgctcgggcg
      841 cggggacgtg aggtcagtcg ccgctgagcc agcgcgaagc agccgtcgac caggagggcg
      901 gtcgcggcga tgaggaccgc cccggcggtg atctgggcca agtcgcggct ggcgtacccg
      961 tcgatgatgt agcgtcccag cccaccgagg ccgacgaacg ctgcgacgga cgtggtggcg
     1021 atgacctgcg cggacgcgga gcgcagcccg gtcacaatca ggggtagggc caccgggact
     1081 tccacccgcc acagccgttg gctgctcgtc agccccatcc cggcggcggc gtctctcacg
     1141 gcctcgtcga cgctgcgcag gccggcgtcg gtgttgagca ggatcggggg cacggcgata
     1201 gctacgagcg cggccagcac cggccacacc gacaagggcg cgagccggaa caccagcacg
     1261 acgacaccca gggttggcaa cgcgcgccag tagttagaca gcgccgcggc cgcgaagctg
     1321 ccgcggccgg tatgcccggt gaggagcccc agcggcaacc cgatgcccgc gccgaggacg
     1381 atcccgacga tcgagtacca caagtgctcg agcaggcgca ttggtacgcc gtccgggccc
     1441 ctccacatgg ccgggtcgag gagccagtcg acaaatgcgg gcacgtcacc tcgccgccct
     1501 ggtccaggga gcgatgccgg cggcagcggc gagcagcagc aggtcggcga cgagcgctag
     1561 cgcgacggtg agcaccagtc cggcccagat ctcaatcggg ttgtcaatct ggaagccgtg
     1621 aatgaacagc tgtccgagcc cgccggtgcc gatgacggca ccgaggctga ccagggagat
     1681 ggtcgacacg gtcgccgctc gcaggccggc gaggagcgcc ggggtggcga gcggtagctc
     1741 gacgcgtacg acgcgtgccc actggcggta tcccatcgcg gtagcggcct gaacgacgtc
     1801 tcggggcacg gcgcgcagcc cctcgacgag gttggcgacg aggatgacca gcgagtacga
     1861 ggccaacgcg aacaccacgg gaagcggtcc gagaccgacc aggctgacca ggagcacgaa
     1921 catcgccagc gccgggaccg tgtacaagat ctgggtcgcg gcccgcatcg gcgggtacag
     1981 gcgcggccac tggtaggcgg ccaaccc
//
LOCUS       DASUPW010000362         2431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1054112, whole genome shotgun sequence.
ACCESSION   DASUPW010000362 DASUPW010000000
VERSION     DASUPW010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054112"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VFY44_08155"
     CDS             <1..531
                     /locus_tag="VFY44_08155"
                     /inference="COORDINATES: protein motif:HMM:NF039581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910452.1"
                     /translation="ERRRDLVESVLLSGDALTADDVATVVGRLAARGRDELGAADAEV
                     RPTYELRYGGQAFELPVEAGPEPDPADLREGFDAAHEERYGYRDPDARLELVTVRVAV
                     ALPGSELEPATAGQAERRGTRSARFGDEALDATVLGPGAAEVEGPAVFELPGSTLVVP
                     PGWTATADGEAVVMRA"
     gene            528..2030
                     /locus_tag="VFY44_08160"
     CDS             528..2030
                     /locus_tag="VFY44_08160"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX5910453.1"
                     /translation="MSDLDAIELQVMLGALRAACDEMGVVLVRSAHSANIKERRDAST
                     ALFDAQGRMVMQAEHIPVHLGAMPSAVAAVMDQEQAPGDAWILNDPFRGGTHLPDITV
                     VSPVFHEDALLGFAASRAHHADVGAPEPGSMPADSRTLDDEGVVIAPTRLARDGELDR
                     ELLGDLTARMRNPGQREADLRAQLAANRAGADRLLALAQRTGSDTLVRAFEQTLDYAE
                     RRTRARIADLDDGEYAATDVLEAREGDLELRLTATVRGDEMVLDFTGSADQHEGNLNC
                     PLAVTLSASYFALRVLTDPDVPACAGAYRPLEVVAPEGCLLNAASPAAVAGGNVETSS
                     RVADLVLAAFGHALGQGTMNNFTLGNDDFTYYETLGGGQGACADADGPDAVHVAMSNT
                     LNTPIEALELEFPLRAVEYAIRRGSGGDGRHRGGEGVVRELEALEPMHYSLITERRRH
                     APPGADGGGPGERGRNSVNGEELPGKASGTLDTGDRLRIETPGGGGRGEH"
     gene            2017..>2431
                     /locus_tag="VFY44_08165"
     CDS             2017..>2431
                     /locus_tag="VFY44_08165"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX5910454.1"
                     /translation="MANTERKAAFLGLGIMGAPMAASLAREGIEVVAWNRTAAKAEAL
                     AGEFDNVTVAGSPAEAAAAAGTVITMVPDAPQVEEVLFGDGGAAEGLDEGGLAIDMST
                     VSPAASRDFGERLAERGIAFLDAPVTGSRPKAEDGT"
BASE COUNT          320 a          877 c          958 g          276 t
ORIGIN      
        1 gagcgccgcc gcgacctggt ggagagcgtg ctgctgtccg gggacgcgct gaccgccgac
       61 gacgtggcca cggtggtcgg ccggctggcc gcgcgcgggc gcgacgagct gggcgccgcc
      121 gacgcggagg tgcggcccac ctacgagctg cgctacggag gccaggcctt cgagctgccc
      181 gtggaggcgg gcccagagcc cgaccccgcc gacctgcgcg agggcttcga cgccgcccac
      241 gaggagcgtt acggctaccg cgacccggac gcgcggctgg agctggtgac ggtgcgcgtc
      301 gcggtcgccc tgcccggatc ggagctggag ccggccaccg cgggccaggc cgagcgccgg
      361 ggcacgcgct ccgcgcgctt cggcgacgag gcgctggacg ccaccgtgct gggcccgggg
      421 gccgcggagg tggagggccc ggcggtgttc gagctgccgg gatcgacgct ggtggtgccg
      481 ccgggctgga cggcgaccgc cgacggagaa gcggtggtga tgcgcgcgtg agcgacctgg
      541 acgcgatcga gctccaggtg atgctgggcg ccctgcgcgc ggcgtgcgac gagatgggcg
      601 tggtgctggt gcgctcggcc cactcggcca acatcaagga gcgccgcgac gcctcgaccg
      661 cgctgttcga cgcgcagggc cggatggtca tgcaggccga gcacatcccc gtgcacctgg
      721 gagcgatgcc cagcgccgtg gccgcggtga tggatcagga gcaggcgccg ggcgacgcct
      781 ggatcctgaa cgaccccttc cgcggcggca cccacctgcc cgacatcacc gtggtctccc
      841 ccgtgttcca cgaggacgcc ctgctgggct tcgccgccag ccgcgcgcac cacgccgacg
      901 tgggcgcgcc cgagccgggc agcatgcccg ccgactcgcg caccctggac gacgagggcg
      961 tggtcatcgc ccccacccgc ctggcccgcg acggcgagct ggaccgcgag ctgctgggcg
     1021 acctgaccgc gcggatgcgc aacccgggcc agcgcgaggc cgacctgcgc gcccagctgg
     1081 ccgcgaaccg cgccggcgcc gaccgcctgc tggccctggc gcagcgcacg gggtcggaca
     1141 ccctggtgcg tgcgttcgag cagacgctgg actacgccga gcggcgcacc cgggcccgga
     1201 tcgccgacct ggacgacggg gagtacgcgg cgaccgacgt gctggaggcg cgcgagggcg
     1261 acctggagct gcggctgacg gcgaccgtgc gcggggacga gatggtgctg gacttcaccg
     1321 gctccgccga ccagcacgag ggcaacctga actgcccgct ggccgtgacg ctgtcggcgt
     1381 cctacttcgc gctgcgggtg ctgaccgacc ccgacgtgcc cgcctgcgcg ggggcctatc
     1441 gcccgctgga ggtcgtcgcg cccgagggct gcctgctcaa cgccgcgtca ccggcggcgg
     1501 tggccggcgg caacgttgag acctcgtcgc gggtggcgga cctggtcctg gccgcgttcg
     1561 gccacgccct cggccagggc acgatgaaca acttcacgct gggcaacgac gacttcacct
     1621 actacgagac cctcggcggc ggccagggcg cctgcgcgga cgcggacggc ccggacgcgg
     1681 tgcacgtggc catgtccaac acgctgaaca cgccgatcga ggcgctggag ctggagttcc
     1741 cgctgcgggc ggtcgagtac gcgatccggc gcggatcggg gggcgacggg cgccaccgtg
     1801 gcggcgaggg cgtggtgcgc gagctcgagg ctctggagcc gatgcactac tcgctgatca
     1861 ccgagcgccg ccgccatgcg cccccgggtg ccgacggcgg cggcccgggc gagcgcgggc
     1921 gcaacagcgt gaacggcgag gagctgcccg gcaaggcttc gggcacactc gacaccggag
     1981 accggttgag gatcgagacc cctggaggtg gcggacgtgg cgaacactga gcggaaggca
     2041 gcgtttctgg gcctgggcat catgggcgcg ccgatggccg ccagcctggc ccgcgagggc
     2101 atcgaggtcg tggcctggaa ccgcaccgcc gcgaaggccg aggcgctggc cggcgagttc
     2161 gacaacgtga ccgtggcggg ctcgcccgcc gaggcggcgg ccgcggccgg caccgtgatc
     2221 acgatggtgc ccgacgcgcc ccaggtcgag gaggtcctgt tcggcgacgg cggcgcggcc
     2281 gaggggctcg acgagggcgg cctggccatc gacatgtcca ccgtctcccc cgcggccagc
     2341 cgcgacttcg gcgagcgcct ggccgagcgc ggtatcgcct tcctggacgc gccggtcacg
     2401 gggtcgcggc ccaaggccga ggacggcacc t
//
LOCUS       DASUPW010000363         3811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_223893, whole genome shotgun sequence.
ACCESSION   DASUPW010000363 DASUPW010000000
VERSION     DASUPW010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223893"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..925)
                     /locus_tag="VFY44_08170"
     CDS             complement(134..925)
                     /locus_tag="VFY44_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX5910455.1"
                     /translation="MSYETVEWQQDGGVGRITLDRPETVNAWNEQFADDMKAVLALAA
                     DESVRVVLIRGAGKGFSSGADLKDGGLTPAEDGKPDILRELHDNYHPVLRGVRELEKP
                     VVAAVHGPAVGIGLSLALACDLILAAESSFMSLAFAGIGLMPDGGSTLFVPAAVGKAR
                     AFEMALLGERVPAPKALEWGLVNAVHPDDELFAKADELVQRLAVGPTRSYAGSKKALN
                     ASIYGHLESQFDLEADLQHALARTDDFVEGVMAFLQKRQPEFKGQ"
     gene            951..1598
                     /locus_tag="VFY44_08175"
     CDS             951..1598
                     /locus_tag="VFY44_08175"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5910456.1"
                     /translation="MLLDSLQWLGHAGFRLQAGGVTVYIDPYRVPDGPPADVILVTHG
                     HYDHFSPQDIERLSGDQTWLVAPAAVAERAKGHVTSIAPGQALELEPFPGIEVAALAA
                     YNTSKRDADGNAFHPRSAGCVGFDVNVRGQRLYHAGDTDVIPEMDSVVGADVALLPVS
                     GTYVMTAKEAAEAARRIQPRVAVPMHWGEHIGSRQDAETFAELAPVEVRIMEPAA"
     gene            complement(1579..2454)
                     /locus_tag="VFY44_08180"
     CDS             complement(1579..2454)
                     /locus_tag="VFY44_08180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5910457.1"
                     /translation="MTAPADPFAGAGLTALGLAAARSVETGRPDRLIEDPFARALYDA
                     AGRDLGMRLEWPDDPGSVTDTEALHLHGSRYIGLRTRFYDDVLLRAAEAGAGQAVLLG
                     AGLDTRSRRLGLPGSLTLFEVDRPAVLAFKEEALAGAPATCRLVGVGADLGEGWHEAL
                     DAAGLDPGVPTVWMAEGLMPYLDEAAQLALLDAVARRAPEGSGVAFDQVAGGDVEALS
                     RRSGIDMEGLLAGTGGTGGLAGALRGRGWSVEVEGADAVAARYGRDLSDPFGGPGGDP
                     PWLRTDFVRATRPAP"
     gene            2507..3421
                     /locus_tag="VFY44_08185"
     CDS             2507..3421
                     /locus_tag="VFY44_08185"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5910458.1"
                     /translation="MRSSRALRTVAWGVVAVGIAAPLVRHRLRLRVPVVTALSFSAPV
                     ALALAAPRTRARDAGIYALQMWAYYAHYDMPDDRPEDLLARTRVGYPARIDGVLGRGR
                     LPTHRLQAALGRPGEIRRHDLALSAVHWSWFLVPHGTAAYMLLFHHDTFVRSAILIAG
                     TFDAGLLVYWALPTAPPWYAAQTGRIPPVRRIMVEAGERFWKGLWQPLYDGLEGNPFA
                     AMPSLHFGTSVMAAHVLSGVGRLHGAVGWTYALTLGFGLVYLGEHYVIDLIAGLALAE
                     GVRRLEPHARPLLLAAGRKIQQLEPGAA"
     gene            3421..>3811
                     /locus_tag="VFY44_08190"
     CDS             3421..>3811
                     /locus_tag="VFY44_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910459.1"
                     /translation="MWLVPNPSNLRKRGEDDGSPDELDTDEHLAEFEEDEEEDHRVAH
                     LLSDHRQLASVAAICVLGVLAIYLVVPKVVGFDESLDRIHDAKWYWVVVAVAFCVAQF
                     VAYVALFRGVLGGRRQTEVRERLDVKAS"
BASE COUNT          509 a         1428 c         1355 g          519 t
ORIGIN      
        1 gccatcgcgc ccggggccag gaccagcagg gccaggacgg cggcagggac gatcaaccag
       61 atggaacggc gcgagcgcaa ggccgcggca tcatatccct cggtcggctc gtaaagccac
      121 ttcgtggcgg gtgtcactgg cccttgaact caggctggcg cttctgcagg aaggccatga
      181 cgccctcgac gaagtcatcc gtgcgcgcca gggcgtgctg gaggtcggcc tcgaggtcga
      241 actggctctc caggtggccg tagatgctgg cgttcagcgc cttcttcgag cccgcgtagg
      301 agcgtgtcgg ccccacggcc aggcgctgga ccagctcgtc ggccttcgcg aacagctcgt
      361 cgtcggggtg cacggcgttc accaggcccc attcgagcgc cttcggagcg ggcacccgct
      421 cgccgaggag ggccatctcg aaggcccgcg ccttgccgac ggccgcgggg acgaagaggg
      481 tggatccgcc gtcgggcatc agcccgatgc cggcgaaggc caggctcatg aacgaggact
      541 cggcggccag gatcaggtcg caggccagcg ccagcgacag cccgatgccc accgcgggac
      601 cgtgcaccgc ggccacgacc ggcttctcga gctcgcgcac gccgcgcagc accgggtggt
      661 agttgtcgtg cagctcacgc aggatgtccg gcttgccgtc ctcggcgggg gtcagcccgc
      721 cgtccttgag gtcggcgccc gaggagaacc ccttccccgc gccacggatg agcacgacgc
      781 gaacggactc gtcggcggcc agcgcgagca cggccttcat gtcatcggcg aactgctcgt
      841 tccaggcgtt gacggtctcg ggccggtcca gcgtgatccg gccgacaccg ccgtcctgct
      901 gccactcgac ggtttcgtag ctcatggggg cgaaaactat cctcaatcgc atgctgctcg
      961 actcgctcca gtggctcggg cacgcgggct tccgcctgca ggccggaggc gtgaccgtct
     1021 acatcgaccc ctaccgcgtg ccggacgggc cgcccgcgga cgtgatcctg gtcacccacg
     1081 gccactacga ccacttcagc ccgcaggaca tcgagcgcct gtccggcgac cagacctggc
     1141 tcgtcgcgcc cgcggccgtg gccgagcggg cgaagggcca cgtcacgtcc atcgcccccg
     1201 gccaggcgct ggagctcgag cccttccccg gcatcgaggt ggccgccctg gccgcctaca
     1261 acacgtccaa gcgcgacgcc gacggcaacg ccttccaccc gcgctcggcc gggtgcgtgg
     1321 gcttcgacgt gaacgtccgc ggccagcggc tctaccacgc aggggacacc gacgtgatcc
     1381 ccgagatgga ctccgtggtg ggagccgacg tcgccctgct gccggtcagc ggcacctacg
     1441 tgatgacggc caaggaggcc gccgaggcgg cgcgccggat ccagccgcgc gtcgcggtgc
     1501 cgatgcactg gggcgagcac atcgggagcc ggcaggacgc cgagaccttc gccgagctgg
     1561 cgcccgtgga ggtccggatc atggagccgg ccgcgtagcg cgcacgaagt ccgtgcgcag
     1621 ccagggcgga tcgccgcccg gcccgccgaa cgggtccgac aggtcgcgcc cgtacctggc
     1681 ggcgaccgcg tccgcgccct cgacctccac gctccagccg cgcccgcgca gcgctccggc
     1741 caggccgccg gtgcctcccg tgccggccag caggccctcc atgtcgatcc ccgaccgccg
     1801 cgacagggcc tcgacgtcgc cgccggcgac ctggtcgaag gccacgccgc tgccctccgg
     1861 cgcgcgccgg gcgaccgcgt ccagcagcgc cagctgcgcc gcctcgtcga ggtagggcat
     1921 gagcccttcg gccatccaca ccgtgggcac gccagggtcg aggccggccg cgtcaagcgc
     1981 ttcgtgccag ccctcgccga ggtcggcgcc gacccccacc aggcggcacg tggccggcgc
     2041 cccggccagg gcctcctcct tgaacgccag gacggcgggc cggtcgacct cgaagagcgt
     2101 gagcgatccc ggcaggccga gccgccgcga tcgggtgtcc agccccgcgc ccagcagcac
     2161 cgcctggccg gctcccgcct cggccgcgcg gagcaggacg tcgtcgtaga agcgggtgcg
     2221 cagcccgatg tagcgcgatc cgtgcaggtg cagcgcctcc gtgtcggtga ccgagcccgg
     2281 gtcgtccggc cactccagcc gcatgcccag gtcgcgcccg gcggcgtcgt agagcgcacg
     2341 cgcgaacggg tcctcgatca ggcggtcggg gcggcccgtc tccaccgagc gcgcggcggc
     2401 gagcccgagc gccgtcagcc cggccccggc gaacgggtcg gcaggagcag tcaccccgca
     2461 caccgtagga gaacgcagcg ccggtcaggc ggtagcttca ccggccgtgc ggtcctcgcg
     2521 tgcattgaga acggtggcct ggggtgtggt tgccgtgggc atcgccgccc cgctggtccg
     2581 tcaccggctg cgcctgcgcg tgccggtggt cacggcgctg agcttcagcg cccccgtcgc
     2641 cctggcgctg gcggcccctc gtacacgcgc gcgggacgcg ggcatctacg cgctccagat
     2701 gtgggcctac tacgcccact acgacatgcc cgacgaccgc cccgaggacc tgctggcgcg
     2761 cacccgcgtg ggctacccgg cgcggatcga cggcgtgctg gggcgcgggc gcctgcccac
     2821 gcaccgcctc caggccgccc tgggccggcc cggcgagatc cggcgccacg acctggcgct
     2881 gtcggcggtg cactggagct ggttcctggt gccccacggc acggccgcct acatgctcct
     2941 gttccaccac gacacgttcg tgcgctcggc gatcctgatc gcgggcacgt tcgacgccgg
     3001 gctgctggtc tactgggcgc tgcccacggc cccgccgtgg tacgcggcgc agaccggccg
     3061 gatcccgccc gtgcggcgga tcatggtcga ggccggcgag cggttctgga aaggcctctg
     3121 gcagcccctt tacgatggtc tggagggcaa tcccttcgct gccatgcctt ccctgcactt
     3181 cggtacctcc gtgatggctg cccacgtcct gtcgggggtc ggtcgcctgc acggtgccgt
     3241 gggctggacc tacgccctga cgctgggctt cggcctggtg tacctgggcg agcactacgt
     3301 gatcgacctg atcgccggcc tggcgctggc cgagggggtg cggcgcctgg agccccacgc
     3361 gcgcccgctc ctgctggccg ccggccgcaa gatccagcag ctggagcccg gagcggcctg
     3421 atgtggctcg tcccgaaccc gtcgaacctc cgcaagcgcg gcgaggacga cggttcgccg
     3481 gacgagcttg acaccgacga gcacctggcc gagttcgagg aggacgagga ggaggaccac
     3541 cgcgtcgccc atctgctctc cgaccaccgc cagctggcca gcgtggcggc catctgcgtg
     3601 ctgggcgtgc tggccatcta cctggtggtg cccaaggtcg tcggcttcga cgagagcctg
     3661 gaccgcatcc acgacgccaa gtggtactgg gtggtggtcg cggtggcctt ctgcgtcgcg
     3721 cagttcgtgg cctacgtcgc gctcttccgc ggggtgctcg ggggccgcag acagaccgag
     3781 gtgcgcgagc gcctggacgt gaaggcctcg t
//
LOCUS       DASUPW010000364         4052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1747076, whole genome shotgun sequence.
ACCESSION   DASUPW010000364 DASUPW010000000
VERSION     DASUPW010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747076"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VFY44_08195"
     CDS             <1..393
                     /locus_tag="VFY44_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910460.1"
                     /translation="AVLVVPCPEDVPAKLHSLREYQGDKIVAVYDPEDCSTLELEFAY
                     RVARMRVTAAREGSDDIDAAAVRATVARAVAAMDDVRKIKSQLTSAQNGIGGAKELLD
                     AVEARVRAELDQVDAALAAANDQAELPL"
     gene            complement(507..1457)
                     /locus_tag="VFY44_08200"
     CDS             complement(507..1457)
                     /locus_tag="VFY44_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910461.1"
                     /translation="MVVLIALVTAFSSASVQAAKPLALHPRLLTAAGPPPQPRAFAGR
                     YVVTVADGYIRLTDDETGRRYTQSLPRSECYATDVRFPELLLGCRPRAATDTRPKILN
                     MLDGSVTEFRDATEYPTAFGQPFEFGVLSDDSFSDLGSRWIGGTASDLVSRSSHPVYV
                     DRLTQRRVNRSRSAPVYDLDSPDLSAKRQACAGPGRTGSGYLAPVDPSAHIWLWLRTT
                     RKDLGGDLFIQRCGGARRLVSRCPSGCQNARFTNRFVTWVSSRGANVRSRRTGRAYTL
                     REAYGPVQVSATDRRMLVSSTGWTSTTSRTVEIPLPASIR"
     gene            complement(1514..1945)
                     /locus_tag="VFY44_08205"
     CDS             complement(1514..1945)
                     /locus_tag="VFY44_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910462.1"
                     /translation="MRVLGKTSAGGLALLACGAAFLSACGEDDFKNDPRPAAAVNLTG
                     VIQPKGVTISPNNRGAGPFLITVSNQTDEAHTVTLEGDSVEETVGPIQPQDTATIQRT
                     LKPGTYEVRAGSERAVVKEIPPFELTVGKPRKSSADQLLLP"
     gene            complement(1960..2898)
                     /locus_tag="VFY44_08210"
     CDS             complement(1960..2898)
                     /locus_tag="VFY44_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5910463.1"
                     /translation="MTTEAGAEEPRPPRPAYDPHDDPLQMMREAPQAVVDTDPRRLRQ
                     LIPVEDETRALDDWGRSERVFDALQPVLDFYYRYWFRVEVEGIENVPSEGGALLTSNH
                     SGALPPDAPMIMQAIRHEHASPRPVYMLAEHWFKGYPGVGMLTNKIGVVPAHPANAQR
                     LLADEERLALVFPEGQKGSRKLYWQRYRLRRFGRGGFVRTAMRAGVPIVPIAVVGAED
                     AMPIFAHVPLLQRLTGLIYFPVNHAFPHFGLAAGAMYLPAKFKIRFLEPVDVSGYSAA
                     DAEDLSLVQNIAEDIRASIQAEVDDLVSSRTSVWFG"
     gene            complement(3011..3964)
                     /locus_tag="VFY44_08215"
     CDS             complement(3011..3964)
                     /locus_tag="VFY44_08215"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX5910464.1"
                     /translation="MPAGYPQGVRRLDRMIASADYELLAEQAGVVAREGRAVLELTGS
                     EAAEFLQGQVSNDVEALEPGQGCYATLLDHKGKLRTDMRVLRLAPDRLLVDAEPIGRR
                     PLAHNFETYSLGRDVKPRDVTDDHVVLSLVGPRAHELLTDPPPREEHACSEADGVVRV
                     STDVGVDLIVPATREAEVRAGLDAPDADEQAAEVLRVERGRPRLGLDMDATTMPQEAG
                     INERAVSFTKGCYVGQETVARLHYKGKPNRHMRGLKLTAPAQHGDPILLGEKQVGTVG
                     SVADSPAHGPIALALVRREAEPGAEVQVAGTPATVVELPFG"
BASE COUNT          555 a         1403 c         1509 g          585 t
ORIGIN      
        1 gccgtgctgg tcgtgccctg ccccgaggac gtgccggcca agctgcactc gctgcgcgag
       61 taccagggcg acaagatcgt ggccgtctac gaccccgagg actgctcgac cctcgagctg
      121 gagttcgcct accgcgtggc ccgcatgcgc gtgaccgcgg ctcgcgaggg ctcggacgac
      181 atcgacgccg cggcggtgcg cgccaccgtg gcccgggcgg tcgccgccat ggacgacgtg
      241 cgcaagatca agagccagct gacctcggcg cagaacggca tcggcggcgc caaggagctc
      301 ctggacgcgg tcgaggcccg ggtgcgggcc gagctggacc aggtcgacgc ggcgctcgcc
      361 gccgcgaacg accaggccga gctgccgctc tagccctggc gcgtctccgg cgcgccggag
      421 cgggcggtcc ttggggcagc gcctcatgct tttcggtcgc tatcacgacc cacacgcatg
      481 aaccgaggtg tggaaggacc gcccggctaa cggatcgagg cggggagggg gatctcgacg
      541 gtgcgggagg tggtgctggt ccagccggtc gacgacacca gcattcgccg gtcggtcgcg
      601 ctcacctgga ccgggccgta cgcctccctc agcgtgtagg cacgcccggt ccggcgggag
      661 cggacgttgg cgccgcggct gctgacccag gtcacgaacc ggttggtgaa tcgggcgttt
      721 tggcagcccg atggacagcg gctgacaagc cgccgggctc cgccgcaacg ttggatgaac
      781 aggtcaccgc ccaggtcctt gcgcgttgtt ctgagccaga gccagatatg cgcggacggg
      841 tccaccggcg ccagatagcc gctcccggtt cgcccggggc cggcgcacgc ctggcgcttg
      901 gcgctcaggt cgggggagtc caggtcgtag acgggtgccg accgcgatcg gttgacgcgc
      961 cgctgggtga gccggtcgac gtagaccggg tgcgagctgc gcgagaccag gtcggacgca
     1021 gtgcctccga tccagcggga gcccaggtcg gagaacgagt cgtccgagag cacgccgaat
     1081 tcgaacggct ggccaaaggc ggtcgggtac tccgtcgcat cgcggaactc ggtcacgctc
     1141 ccgtcgagca tgttcaggat cttcgggcgc gtgtcggtgg cggcgcgtgg ccggcagccg
     1201 agcaggagct ccgggaacct gacatccgtc gcatagcact cggaccgggg caggctctgg
     1261 gtgtaccggc ggccggtctc gtcgtcggtg aggcggatgt agccgtccgc caccgtgacg
     1321 acgtacctgc cggcgaacgc ccggggctgc gggggcgggc cggccgcggt caggaggcgg
     1381 gggtggagcg cgagtggctt cgcggcctgc accgaagcgc tgctgaacgc cgtcacgagt
     1441 gcgatcagaa cgaccaaccc cccgagtctg ctcatggcag ctccgacacc tgccgggcgc
     1501 gggagttgcg ccgctacggc agcagcagct ggtcggccga cgacttgcgc ggcttgccga
     1561 ccgtgagctc gaacggcggg atctccttga ccaccgcgcg ctcggagccg gcacggacct
     1621 cgtaggtgcc gggcttgagg gtgcgctgga tcgtggccgt gtcctgaggc tggatcgggc
     1681 cgacggtctc ctcgacggag tcgccctcca gcgtgaccgt gtgcgcctcg tcggtctggt
     1741 tggagaccgt gatcaggaac ggaccggcgc cgcggttgtt ggggctgatg gtgaccccct
     1801 tgggctggat cacgccggtc aggttcacgg ccgccgcggg acgcgggtcg ttcttgaagt
     1861 cgtcctcacc gcaggcggac agaaacgccg cgccgcacgc gagcagcgcc agccccccgg
     1921 ccgaagtctt ccctaacacc ctcatccctg gccgccaccc taaccgaacc acacggacgt
     1981 tcgcgaagac acgaggtcgt cgacctcggc ctggatgctc gcgcggatgt cctccgcgat
     2041 gttctggacc agcgacagat cctccgcgtc ggcggccgaa tagcccgaca cgtcgaccgg
     2101 ttccaggaag cggatcttga acttcgccgg caggtacatc gcgcccgccg cgaggccgaa
     2161 atgggggaag gcgtggttga ccgggaaata gatcagcccg gtcaggcgct gcagcagcgg
     2221 cacgtgggcg aagatgggca tggcgtcctc ggcgccgacg accgcgatgg gcacgatcgg
     2281 caccccggcg cgcatggcgg tgcgcacgaa cccgccgcgg ccgaagcggc gcaggcggta
     2341 gcgctgccag tacagcttgc ggctgccctt ctggccctcg gggaacacca gcgccaggcg
     2401 ttcctcgtcc gccagcagcc gctgcgcgtt ggccgggtgc gcgggcacca cgccgatctt
     2461 gttggtcagc atgcccacgc ccggatagcc cttgaaccag tgctcggcca gcatgtacac
     2521 gggccgcggc gaggcgtgct cgtggcggat ggcctgcatg atcatggggg cgtcgggggg
     2581 cagggcgccg gagtggttgg acgtcagcag cgcgccgccc tcggagggca cgttctcgat
     2641 gccctcgacc tcgacccgga accagtaccg gtagtagaag tcgagcacgg gctggagcgc
     2701 gtcgaacacg cgctcggagc ggccccagtc gtccagcgcc cgggtctcgt cctcgaccgg
     2761 gatcagttgg cgcaatcggc gtgggtcggt gtcgaccacc gcctgcgggg cctcgcgcat
     2821 catctgcagc gggtcgtcgt gcgggtcgta ggcaggccgg ggcgggcgcg gctcctccgc
     2881 acccgcttcc gtggtcaagg gcccgcccag gagtggactg tcgtcgctca gttgcccccg
     2941 cgccggccga cgctgtcgct gatcggccat cgggcgatgc tagcccgctc cgcggcgggg
     3001 tgggctgcgc tcagccgaag gggagctcga cgaccgtggc cggcgtgccc gccacctgca
     3061 cctcggcgcc gggctcggcc tcgcgccgca ccagcgccag ggcgatgggg ccgtgggcgg
     3121 gggagtcggc caccgagccg acggtgccca cctgcttctc gccgagcagg atcgggtcgc
     3181 cgtgctgggc cggggcggtc agcttcaggc cgcgcatgtg ccggttgggc ttgcccttgt
     3241 agtgcagccg ggccaccgtc tcctggccca cgtagcagcc cttggtgaag ctcacggcgc
     3301 gctcgttgat gcccgcctcc tggggcatgg tggtggcgtc catgtccagc cccaggcggg
     3361 ggcggccacg ctcgacgcgc agcacctcgg cggcctgctc gtcggcgtcg ggggcgtcca
     3421 ggccggcccg cacctcggcc tcgcgcgtgg ccggcacgat caggtcgacg ccgacgtcgg
     3481 tgctcacgcg caccacgccg tcggcctcgg agcacgcgtg ctcctcgcgc gggggcgggt
     3541 cggtcagcag ctcgtgcgcg cgcgggccca ccagcgacag caccacgtgg tcgtcggtca
     3601 cgtcccgcgg cttgacgtcg cggcccaggc tgtacgtctc gaagttgtgc gccagcgggc
     3661 ggcggccgat gggctcggcg tccaccagga ggcggtcggg ggccaggcgc agcacgcgca
     3721 tgtccgtgcg cagcttgccc ttgtggtcca gcagggtcgc gtaacagccc tgcccgggct
     3781 ccagcgcctc gacgtcgttg gacacctggc cctgcaggaa ctcggcggcc tcggagcccg
     3841 tcagctccag caccgcgcgg ccttcccggg ccactacgcc ggcctgctcg gccagcagct
     3901 cgtagtcggc ggaggcgatc atgcgatcga gtctacgcac gccctggggg tagcctgcgg
     3961 gcatcgagtc aggcggttca caggaggcgc tgtcccagag cggcgcggtg tccgaggcgg
     4021 tccaggacta cgcgaaggcc atctattcgc tg
//
LOCUS       DASUPW010000365         5204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_362644, whole genome shotgun sequence.
ACCESSION   DASUPW010000365 DASUPW010000000
VERSION     DASUPW010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5204
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362644"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2284
                     /locus_tag="VFY44_08220"
     CDS             <1..2284
                     /locus_tag="VFY44_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910465.1"
                     /translation="QLANGIPGIGNLPGGGADPNVATACERIAFRAVEILGGENGCFS
                     SVPGEKGVKVSEGTLRVNGLEIIPDPGVKIRIDTKGAKTIDTVTASGAPGRVTVQIRV
                     SGSSPIVLFRGPLHLQLPTGAAGTKLATFAPGGVNIKGFPVTGSFDVILKQDAVEIPI
                     EIGLPKSLLDITAGVTLRADNDRGLRVDSMRFKLPKVAIGPLNLANLDVSWTGSSDQW
                     RGTAELNLFKAGAKVAVQFDKGRFTDGSITITPVPYPGINLAPDTYLNNVFGRLRLLD
                     NESFAEAGALFGVQPVSPPGTPNGTYVVTVRGSLRVNWRPAFLLTLGGESFLGGEIPL
                     AQAKVTADLDGNFTGSGQAQFKVPLVRGDGKFNAFIGAGTGKFAASIDANVTVGTSPL
                     TVKVGTKAGFSNDEIYGCSSPIGGFKYTFANRDFTPYVFTCPGEPEVPSFVLDALRKD
                     PAALKKFYDDRGIPQPAPAGQQARVAQAGTPGFDVPRGVSGVSVTAIGFNGAPGFTLV
                     SPTGQSVVPQPANAPNGAAAVYTRTVFRTIVGLRKPAPGRWRMVEASPGSIATVNMAR
                     ELPKPKVRIKVTGKGRKRTLRYTATARKGLAIKLFERMGKGGHEIGTITKRRGTIRFR
                     VADGRGGRRAIEAHAEQRGLPVSQVKVGSYRAPAPARPARVRGLRVGRSGRSAIVRWR
                     RARGATTYLVRVKVSKGTKMRVVRLVSKRSLRVRRVPKTSIVSVSVTARNAKGRAGKA
                     AKAKSKPRKSKSKGKRKKRK"
     gene            2331..4136
                     /locus_tag="VFY44_08225"
     CDS             2331..4136
                     /locus_tag="VFY44_08225"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5910466.1"
                     /translation="MIDIGTIVGGYRVERMLGRGGMGVVYEAVQVSLDRRVALKVLRP
                     ELAEDTAFVDRIRREGMVQAALEHPHVLDVYEVGEAEGELFLTMRLVDGATLAELLRA
                     GTLDTERALTLLDQVAGALDAAHEAGLLHRDVKPQNVLVADDGGAFLTDFGLSRAASD
                     TVTASRPTLGTVAYIAPEVIRGEAPTPASDRYSFAATLFHCLSGDVVFPRGSDIAVIY
                     AHTNEAPPRISERRPGLPKELDHLFEAALAKDPADRPASAKDLVGGVRDALGDEAGAE
                     LGAPVPPAPPPAAGVPPSAGTPSRRRWIAAIVATGIGAAALGAGAAALSDDGRHRPEV
                     PVPPPAKGAQVIGSELGAPDRSLDCRGDEPRGRSVACSIVQARNAGAKLIVPADGTIV
                     GWSVRGARGEMALDVIRPSGEDTIRVNRSPFETAGNAGPHRFRTDMAVERGDLVGLQL
                     GPGARIGVRDADGATTRRWFGVVGGFYGSADRGPGTGFDHEVLVRAEFVAGRKQALPR
                     KLTGAAAADAPAGKLRLRERVPISSPRGSVTAEVREVGQRVVIDLVRGGRRVQRQFLP
                     DLRPDGNPAQLEALPVEGEPYGESTCAGSTRTAGV"
     gene            4198..>5204
                     /locus_tag="VFY44_08230"
     CDS             4198..>5204
                     /locus_tag="VFY44_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5910467.1"
                     /translation="MIAGFRVERLLGEGEMGPVYEAVQLSLGRPVALRALAGSGPAGA
                     SVDHPNIVPVYEAGEWEGRRFVVSRLVRGESLAELLDAGSLSPDRFDEIMDAVSGAVA
                     AAHADGLVHGRLAAHNVIVDRAGTPFVTDFGLGRAGTTEGDRQALAALWSRRPMRARR
                     PAVVAAAAVLGVAVVVGLLLVAARDGADTGAAKPVESTGCAPDPDPNTPACTMSQGMV
                     GGRPVMVRADGAVRRWAVRGAAGDLSLQVLRREDGRTVRAGFSQPARVRGPGPHAFPV
                     NLDVRRGDRIGVLLAPGARIGLRPRTGATTQRWDGRRTPLPRPSTLAGQLLLRADVRP
                     GA"
BASE COUNT          725 a         1823 c         1960 g          696 t
ORIGIN      
        1 ccagctggcc aacggcatac cgggcatcgg caacctgccc ggtggcggcg cggaccccaa
       61 cgtggccacc gcgtgtgagc ggatcgcgtt ccgggcggtc gagatcctgg gtggcgagaa
      121 cggctgcttc tccagcgtgc cgggcgagaa gggggtcaag gtcagcgagg gcacgctgcg
      181 cgtgaacggc ctggagatca tccccgaccc gggcgtgaag atccggatcg acaccaaggg
      241 cgccaagacc atcgacaccg tgaccgcgtc gggcgccccc ggcagggtca ccgtgcagat
      301 ccgggtgtcg ggctcatctc cgatcgtgct cttccgcggg cccctgcacc tccagctgcc
      361 cacgggagcg gcgggcacca agctcgccac gttcgccccc ggtggcgtga acatcaaggg
      421 cttcccggtc accggctcgt tcgacgtgat cctcaagcag gacgccgtcg agatcccgat
      481 cgagatcggg ctgcccaagt cgctgctgga catcaccgcc ggcgtgacgc tccgggccga
      541 caacgaccgc ggcctgcggg tggactcgat gcgcttcaag ctgcccaagg tggccatcgg
      601 gccgctgaac ctggccaacc tggacgtgtc gtggacgggc tcgagcgacc agtggagggg
      661 cacggccgag ctgaacctgt tcaaggcggg cgccaaggtc gcggtgcagt tcgacaaggg
      721 gcggttcacc gatggctcga tcaccatcac gccggttccg taccccggca tcaacctggc
      781 gccggacacc tacctgaaca acgtcttcgg gcgattgcga ttgctcgaca acgagtcgtt
      841 cgcggaggcg ggcgcgctgt tcggcgtgca gcccgtgtcc ccgccgggga cgcccaacgg
      901 cacctacgtg gtcacggtgc ggggctccct gcgggtcaac tggcggcccg ccttcctgct
      961 cacgctgggc ggagagagct tcctgggcgg cgagatcccg ctggcgcagg cgaaggtgac
     1021 ggccgacctg gacggcaact tcaccggctc ggggcaggcc cagttcaagg tcccgctggt
     1081 ccggggcgac ggcaagttca acgccttcat cggggcgggg acgggcaagt tcgccgccag
     1141 catcgatgcg aacgtgaccg tcggcacctc gccgctgacc gtcaaggtcg gcaccaaggc
     1201 gggcttctcg aacgacgaga tctacggctg ctcgtcgccg atcggagggt tcaagtacac
     1261 cttcgccaac cgcgacttca cgccgtacgt gttcacctgc cccggggagc cggaggtccc
     1321 gtcgttcgtc ctcgacgcgc tcaggaagga tcccgcggcg ctgaagaagt tctacgacga
     1381 ccgcggcatc ccccagcccg cgcccgccgg ccagcaggcg cgggtggcgc aggccggcac
     1441 gcccgggttc gacgtcccca ggggcgtgag cggggtctcg gtgaccgcga tcgggttcaa
     1501 cggcgcgccc ggcttcacgc tggtgtctcc gaccggccag tcggtcgtgc cccagccggc
     1561 gaacgctccc aacggcgcgg ccgccgtcta caccaggacg gtgttcagga cgatcgtcgg
     1621 gctgcgcaag ccggcccccg gccggtggcg gatggtcgag gcgtcgccgg gctcgatcgc
     1681 cacggtgaac atggcgcgcg agctgcccaa gcccaaggtg cggatcaagg tcaccgggaa
     1741 gggccgcaag cgcacgctgc gctacaccgc gaccgcgcgc aaggggctgg cgatcaagct
     1801 gttcgagcgg atgggcaagg gcgggcacga gatcggcacg atcaccaagc gacgggggac
     1861 gatccgcttc cgggtggccg acggccgcgg tggccggcga gccatcgagg cccacgccga
     1921 gcagcgcggg ctgccggtgt cgcaggtcaa ggtggggagc taccgggcgc ccgcgccggc
     1981 ccgcccggcg cgcgtgcgcg ggctgagggt cggccgctcg ggccggtcgg cgatcgtgcg
     2041 ctggcggcgc gcgagggggg ccaccaccta cctggtccgg gtcaaggtca gcaagggcac
     2101 gaagatgagg gtcgtgcggc tggtgtccaa gcggtcgctc agggtccgcc gcgtgcccaa
     2161 gacctcaatc gtcagcgtct cggtcaccgc ccgcaacgcc aagggccgcg ccggcaaggc
     2221 ggccaaggcc aagtccaagc cccggaagtc gaagtccaag ggcaagcgca agaagaggaa
     2281 gtaattcgag atcgggctta agctggcccg atgttgcgcg ggggcgacag gtgatcgaca
     2341 tcggcacgat cgttggtggc tatcgcgtcg agcgcatgct cggccgtggc ggcatgggcg
     2401 tggtgtacga ggcagtccag gtctcgctcg accgccgggt cgcgctgaag gtcctgaggc
     2461 ccgagctggc ggaggacacc gccttcgtgg accggatccg ccgcgagggc atggtgcagg
     2521 ccgcgctcga gcacccccac gtgctcgacg tgtacgaggt cggagaagcc gagggggagc
     2581 tcttcctgac catgcggctg gtcgacgggg cgaccctcgc ggagttgctg cgagccggga
     2641 ccctggacac cgagcgcgcg ctgaccctgc tcgaccaggt cgccggcgcg ctggacgccg
     2701 cccacgaggc aggtctcctg catcgcgacg tgaagccgca gaacgtgctg gtcgccgacg
     2761 acggcggcgc cttcctgacc gacttcgggc tcagccgggc ggccagcgac acggtcacgg
     2821 cgtcgcggcc cacgctgggc acggtcgcct acatcgcgcc cgaggtcatc cgcggcgagg
     2881 cgccaacgcc ggcctccgac cggtactcgt tcgcggcgac cctgttccat tgcctcagcg
     2941 gggacgtcgt gttcccgcgc ggctcggaca tcgccgtcat ctacgcgcac accaacgagg
     3001 cgccaccgcg catcagcgag cgccggccgg gcctgccgaa ggagctcgac cacctcttcg
     3061 aggcggcgct ggccaaggac ccggccgatc gcccggcgag cgccaaggac ctcgtcggcg
     3121 gcgtgcggga cgcgctcggc gacgaggcgg gcgccgaact cggagccccg gtgccgccgg
     3181 ctccgccgcc ggcggccggt gtgcctccga gcgccggcac gccctcccgt cgccgttgga
     3241 tcgcggcgat cgtggccacg gggatcgggg ccgccgcgct gggtgcgggc gcggccgcgc
     3301 tctcggacga cggccggcac cggccggaag tgcccgtgcc gcccccggcg aagggcgcgc
     3361 aggtcatcgg gagcgagctc ggggctccgg accgctcgct cgactgtcgt ggtgacgagc
     3421 cacgagggcg ctcggtggct tgctcgatcg tccaggccag gaacgcgggg gcgaagctca
     3481 tcgtgcccgc cgacggcacc atcgtcggct ggtcggtgcg gggcgcgcgg ggcgagatgg
     3541 cgctcgacgt gatccgtccc agcggcgagg acaccatccg tgtgaacagg tccccgttcg
     3601 agacggccgg caacgccggg ccgcaccgtt tccggacgga catggcggtg gagcgcggcg
     3661 acctggtcgg cctccagctg ggaccgggtg cccggatcgg cgtgcgcgac gcggacggtg
     3721 ccaccacccg ccgctggttc ggcgtcgtgg gcgggttcta cggcagcgcc gaccgcggcc
     3781 cgggtaccgg cttcgaccac gaggtgctgg tccgcgcgga gttcgtggcc gggcgcaagc
     3841 aggcgctgcc aaggaagctg accggcgcgg ccgcggccga cgcgcccgca ggcaagctgc
     3901 gcctccgtga gcgtgtcccg atctcttcgc cgcgcggcag cgtcacggcg gaggttcgcg
     3961 aggtcgggca gcgggtggtc atcgacctgg ttcgcggggg aaggcgcgtg cagcgccagt
     4021 tcctgcccga cctccggccg gacggcaacc cggcccagct ggaggccctg cccgtggagg
     4081 gcgagcccta tggggagtcg acctgcgctg ggtcaacccg aacagcgggc gtctgatcta
     4141 ccacggcctg acgatgtccc ggagcgacat ccagtacatc ctctaggccc atccaccgtg
     4201 atcgccggct tccgggtcga gcgcctcctc ggtgaggggg agatgggtcc cgtctacgaa
     4261 gcggtccagc tgtcgctggg taggccggtg gcgctcaggg ccctggccgg gagcggccct
     4321 gccggcgcgt cggtcgatca tccgaacatc gtccccgtct acgaggcggg tgaatgggag
     4381 ggtcgccggt tcgtggtcag ccggctggtc cgaggggagt ccctggccga gctgctcgat
     4441 gccggctcgc tctcgccgga ccggttcgac gagatcatgg acgctgtctc gggcgcggtc
     4501 gcggcggcgc acgctgacgg cctggtccat ggccggcttg cggcccacaa cgtgatcgtt
     4561 gatcgagccg ggacaccgtt cgtcacggat ttcggactcg ggcgtgctgg caccaccgaa
     4621 ggggaccggc aggcgctcgc ggcgctctgg tcgcgccgcc cgatgagagc gcgccgcccg
     4681 gccgtggtgg cggccgcggc cgtgctgggc gtggcggtcg tggtgggcct cctgctcgtg
     4741 gccgccaggg acggggcgga cacgggtgcg gcgaagccgg tcgaaagcac cggttgcgcg
     4801 cccgatcccg atcccaacac ccccgcgtgc accatgagcc agggcatggt cggcgggcgg
     4861 ccggtgatgg tgcgggcgga cggcgccgtt cgccgctggg cggtgcgcgg ggccgcaggg
     4921 gacctcagcc tgcaggtgct gcgtcgcgag gacggacgca ccgtgcgcgc cgggttctcc
     4981 cagccggctc gcgtccgcgg tccgggcccg cacgcgtttc ccgtcaacct ggacgtccgg
     5041 cggggcgacc ggatcggggt gctgctcgca ccgggtgcca ggatcgggct ccgcccgcgc
     5101 accggcgcca ccacgcagcg gtgggatggc aggcggacac cgctgccgcg cccgagcacc
     5161 ctcgccggcc agctgctcct gcgcgccgac gtccggcccg gcgc
//
LOCUS       DASUPW010000366         2647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_84752, whole genome shotgun sequence.
ACCESSION   DASUPW010000366 DASUPW010000000
VERSION     DASUPW010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84752"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1094)
                     /gene="cysA"
                     /locus_tag="VFY44_08235"
     CDS             complement(135..1094)
                     /gene="cysA"
                     /locus_tag="VFY44_08235"
                     /EC_number="7.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HEX5910468.1"
                     /translation="MISVQNVSKNYDDFKALDDVSVEVQPGSLTALLGPSGSGKSTLL
                     RIIGGLEDPDGGAVVINDEEVTPVPPQRRGIGFVFQHYAAFKHMTVRDNVGFGLKIRK
                     RPKDEIAARVDELLGVVGLAGYQGRYPNQLSGGQRQRMALARALAVEPKVLLLDEPFG
                     ALDATVRKELRVWLRRLHDEVDVTTLFVTHDQEEAMDVADQIVVMNEGRIEQVGSPRA
                     LYEEPANEFVMKFVGEVNQVGNELIRPHDLEILTVPDGSAQEGLIDRVVYLGFEVRVE
                     LTLEDGSKVWVQTTRTRAEELELAQGQIVHVRTDAAREFAPAA"
     gene            complement(1091..1891)
                     /gene="cysW"
                     /locus_tag="VFY44_08240"
     CDS             complement(1091..1891)
                     /gene="cysW"
                     /locus_tag="VFY44_08240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEX5910469.1"
                     /translation="MARAGRLSMRFVALAYLAAILLIPVAVIAYKTFEPGFASVWESI
                     TTPAAIHAFWLTIQVTAIAVPLNAVLGVFTALALVRGRMPGKKFVEAIIDLPFAISPV
                     VIGLALVLLYGREGWFGDELTEAGVQVLFSFPGIVLATIFVTLPFVVREVAPVLREVG
                     DEQEQAAATLGASTWTTFRLITLPAIRWGLVYGVVLSVARAIGEFGAVSVVSGKVAGE
                     TETLTLLVEKRFNNFDLAGAYAASTLLAIIALVTLGGMVLANRRKELS"
     gene            complement(1884..>2647)
                     /gene="cysT"
                     /locus_tag="VFY44_08245"
     CDS             complement(1884..>2647)
                     /gene="cysT"
                     /locus_tag="VFY44_08245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEX5910470.1"
                     /translation="IGGAGGLTLGVTMLWLSVIVLLPLAALVTESFQGGLGSFWDSAT
                     TKQAIAALRFTVLISLLVTLINVVMGTLIAWVLVRDEFPGKRAVNAVIDLPFALPTIV
                     AGIVLLALYGDQVANKKVGVLLALLFVTLPFVVRAVQPVLQELDREMEHAAYSLGASR
                     RTTFFRIILPNLVPAIVSGAALAFARAVGEFGSLTLISGNIPFDTQVSSVYAYKQLES
                     DNITGAAAVSVVLLGVSLLILVGLRVFEGRLSRHG"
BASE COUNT          438 a          954 c          904 g          351 t
ORIGIN      
        1 ggcccgccga cctgcgtggg gttgccggtc agcgacgggc tctcggtgat cggcgccgct
       61 gcggccccgc caggggccag gagcatcgac agcgccacga cggcgacggc cacgacccgg
      121 ctcatgggcg gagcctacgc cgcgggggcg aactcgcgcg ccgcatccgt gcgcacgtgc
      181 acgatctggc cctgggccag ctccagctcc tcggcgcggg tgcgggtggt ctggacccac
      241 accttgctcc cgtcctccag cgtcagctcg acccggacct cgaagcccag gtacaccacg
      301 cggtcgatca ggccctcctg cgccgagccg tcgggcacgg tcaggatctc caggtcgtgc
      361 gggcggatga gctcgttgcc cacctggttg acctcgccga cgaacttcat cacgaactcg
      421 ttggccggct cctcgtacag cgcgcggggg ctgcccacct gctcgatcct gccctcgttc
      481 atgaccacga tctggtcggc cacgtccatg gcctcctcct ggtcgtgggt cacgaacagg
      541 gtggtcacgt cgacctcgtc gtgcaggcgg cgcagccaca cgcgcagctc cttgcgcacg
      601 gtcgcgtcca gcgcgccgaa cggctcgtcc agcagcagca ccttgggctc gacggccagc
      661 gcgcgggcca gggccatgcg ctggcgctgg ccgcccgaga gctggttggg gtagcggccc
      721 tggtagccgg cgaggccgac cacgcccagc agctcgtcga cccgagcggc gatctcgtcc
      781 ttgggccgct tgcggatctt cagcccgaag cccacgttgt cgcgcacggt catgtgcttg
      841 aacgccgcgt agtgctggaa gacgaagccg atcccgcgcc gctgcggggg caccggggtg
      901 acctcctcgt cgttgatgac cacggcgccg ccgtcggggt cctcgagccc gccgatgatg
      961 cgcagcagcg tcgacttgcc cgagcccgag gggcccagca gcgccgtcag cgagcccggc
     1021 tgcacctcca ccgacacgtc gtcgagcgcc ttgaagtcgt cgtagttctt gctgacgttc
     1081 tggacggaga tcacgagagt tccttgcgtc ggttggccag gaccatgccg cccagggtga
     1141 ccagggcgat gatggcgagc agggtcgacg ccgcgtaggc gccggccagg tcgaagttgt
     1201 tgaagcgctt ctccaccagc agggtcagcg tctcggtctc gcccgcgacc ttgccggaga
     1261 ccacgctgac cgcaccgaac tcgccgatgg cgcgcgccac cgacagcacc acgccgtaga
     1321 ccagccccca gcggatggcc ggcagggtga tcaggcggaa cgtggtccac gtggacgcgc
     1381 ccagcgtggc ggcggcctgc tcctgctcgt cccccacctc gcgcagcacc ggcgcgacct
     1441 cgcgcaccac gaacggcagc gtcacgaaga tcgtggccag gacgatgccc gggaacgaga
     1501 acagcacctg gacgccggcc tcggtcagct cgtcgccgaa ccagccctcg cggccgtaga
     1561 gcagcaccag cgccaggccg atgaccaccg gcgagatggc gaacggcagg tcgatgatcg
     1621 cctccacgaa cttcttcccc ggcatccggc cacgaaccag cgccagcgcc gtgaacacgc
     1681 ccagcacggc gttcaggggc acggcgatcg cggtgacctg gatggtcagc cagaaggcgt
     1741 ggatggcggc cggggtggtg atggactccc acacggacgc gaaccccggc tcgaaggtct
     1801 tgtaggcgat caccgcgacc gggatcagca ggatcgcggc caggtacgcc agcgcgacga
     1861 agcgcatcga caggcgtccg gctctagcca tggcggctca accgcccctc gaacacccgc
     1921 aggccgacca ggatcagcag cgacacgccc agcaggacca ccgacaccgc cgccgcgccg
     1981 gtgatgttgt cggactccag ctgcttgtag gcgtagaccg acgacacctg ggtgtcgaac
     2041 gggatgttgc ccgagatcag ggtcagcgac ccgaactcgc cgaccgcacg ggcgaaggcc
     2101 agcgcggcgc cggacacgat ggccggcacc aggttcggca ggatgatccg gaagaaggtg
     2161 gtgcgcctgg acgcgccgag ggagtaggcc gcgtgctcca tctcgcggtc cagctcctgg
     2221 agcacgggct gcacggcccg gaccacgaac ggcagcgtca cgaacagcag cgccagcagc
     2281 acgccgacct tcttgttggc cacctggtcc ccgtacaggg ccaggagcac gatgcccgcg
     2341 acgatcgtgg gcagcgcgaa cgggaggtcg atgaccgcgt tgaccgcccg cttgcccggg
     2401 aactcgtccc ggaccaggac ccaggcgatc aacgtgccca tgaccacgtt gatgagcgtg
     2461 accagcagcg agatcaggac ggtgaagcgc agcgcggcga tcgcctgctt ggtggtggcc
     2521 gagtcccaga acgagccgag gccgccctgg aaggactcgg tgaccagcgc cgccagcggc
     2581 agcagcacga tgacgctgag ccacagcatc gtgaccccca gcgtcaggcc gcccgcgccg
     2641 ccgatct
//
LOCUS       DASUPW010000367         2746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_362659, whole genome shotgun sequence.
ACCESSION   DASUPW010000367 DASUPW010000000
VERSION     DASUPW010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362659"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..408)
                     /locus_tag="VFY44_08250"
     CDS             complement(82..408)
                     /locus_tag="VFY44_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910471.1"
                     /translation="MSNHTTHTPDVTVRLARSSDTRQLFSLAALDSASVPQGEVVVAE
                     SEGRIVAALPLAGGPAIADPFHRTLALVQMLELRAQQLRAATPAAVSAAERLRGMFGR
                     PALRTH"
     gene            502..1977
                     /locus_tag="VFY44_08255"
     CDS             502..1977
                     /locus_tag="VFY44_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX5910472.1"
                     /translation="MTDLDRDGDTSITQQLVDRFVAAIESGDLAPGQKLPPTRALASD
                     AGINHLTAARVYRRLAEEGWVTASVGRGTFVRTLAPAAADESGDDWQAYVLPDRPTTY
                     QEEILDDSFRMPGQPGMISMSTGFPSPRFHPVEEMREIAQEVFAKGAEDALAYTAPEG
                     HVALREQIATHVDGEVLDPDQVIVTTGAQQALDIVYRTLLQPSDVAVTESPTFTGSLL
                     AMRATGARVIGVPVDEEGLDVDALEAVLSRHEVRLLSLQATCHNPTGVDLSAERRERL
                     MRLAVDRNMFVVEDRVYSDLRYEGDAPPPLRLSAPGHVLEVNSLSKVVGGGLRIGWIN
                     ARGPVLGRLAAMKQASDFHTPTPVQHMAARYLAGDGYARQLERSLPYYRERRDAIMAA
                     LEKHLAGEYEAASPAGGHHVWVTLKRPVEERTLYAEAVRHGMSFTPGGAVTAERHQRT
                     SLRLSFSLLEPDELEEGVRRLARALREVRRRDRTRVAAPLS"
     gene            1997..>2746
                     /locus_tag="VFY44_08260"
     CDS             1997..>2746
                     /locus_tag="VFY44_08260"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5910473.1"
                     /translation="MLEGREEELAWMATRLEALRRHRPFAALIRGEAGVGKSSLIDAA
                     VGHARGIRVLRARGYESDRDIPFAGLLELASPLLELRDRLPEAQREAVEAALALRQAG
                     PHDRFAVPAGLLGLLSHAAEDEPVVVVVDDVHWLDDASRGALLFVARRLAGEPIGVLL
                     GLRDGVAPALDFSGIEELALRGLPVDSALRVLHGAAPDLPERVARTLVDATAGNPLAL
                     LEIPAALTAEQRAGTDALTDPLPTTTALEGAF"
BASE COUNT          369 a          999 c         1016 g          362 t
ORIGIN      
        1 ctcagctcca ctgaaacacg ccggccgccg gaccctggga ggtccggcgg ccgggtgtgt
       61 ctccctagga ggagggaggg tctagtgggt gcgcagcgcg ggccggccga acatgccccg
      121 caggcgctcg gcggcgctga ccgcggccgg ggtggcggcg cgcagctgct gggcgcggag
      181 ctcgagcatc tgcaccagcg cgagcgtgcg gtggaacggg tcggcgatgg cgggcccgcc
      241 tgccagcggg agcgcggcca cgatccggcc ctcggactcg gcgaccacga cctcgccctg
      301 cggcacggac gcggagtcca gggcggcgag ggagaagagc tggcgcgtgt ccgaggaacg
      361 agccagccgc accgtgacgt cgggtgtatg agtggtgtgg ttggacattg tgacctcttc
      421 tatagaacaa gttgctgctt tgcgagtaca ggggtagtat caacgctcat gggtgctaca
      481 acaatgtcgt acaagttgga cttgaccgat ctcgatcgcg acggcgacac gtccatcacc
      541 cagcagctgg tcgaccgctt cgtggccgcc atcgagtcgg gtgacctggc gcccggccag
      601 aagctgccac ccacccgcgc gctggcctcc gacgccggga tcaaccacct gaccgccgcg
      661 cgcgtgtacc ggcgcctggc cgaggagggt tgggtcacgg ccagcgtcgg ccgcggcacc
      721 ttcgtgcgca ccctggcgcc cgccgccgcc gacgagagcg gcgacgactg gcaggcctac
      781 gtgctgcccg accgccccac cacctaccag gaggagatcc tggacgactc gttccggatg
      841 cccgggcagc cgggcatgat ctccatgtcc accggcttcc ccagcccccg cttccacccc
      901 gtggaggaga tgcgcgagat cgcccaggag gtgttcgcca agggcgccga ggacgcgctg
      961 gcctacaccg cgcccgaggg ccacgtggcg ctgcgcgagc agatcgcgac ccatgtggac
     1021 ggcgaggtgc tcgaccccga ccaggtgatc gtgaccaccg gcgcccagca ggcgctggac
     1081 atcgtctacc gcaccctgct ccagcccagc gacgttgccg tgaccgagtc gcccaccttc
     1141 accggctcgc tgctggccat gcgcgccacg ggtgcgcggg tgatcggtgt gccggtggac
     1201 gaggaggggc tggacgtgga cgcgctcgag gcggtgctgt cccgccacga ggtgcgcctg
     1261 ctgagcctgc aggccacgtg ccacaacccc accggcgtcg atctgtccgc cgagcgccgc
     1321 gagcgcctga tgcgcctggc cgtggaccgc aacatgttcg tggtcgagga ccgcgtgtac
     1381 tccgacctgc gctacgaggg cgacgcgccg ccgccactac gcctctccgc gccaggacac
     1441 gtgctggagg tgaactcgct gtccaaggtg gtcggcggcg gcctgcggat cggctggatc
     1501 aacgcccgcg ggccggtgct gggccgcctg gcggcgatga agcaggccag cgacttccac
     1561 acccccaccc cggtccagca catggccgcc cgctacctgg ccggcgacgg ctatgcgcgc
     1621 cagctggagc gcagcctgcc ctactaccgc gagcgccgcg acgcgatcat ggccgcgctg
     1681 gagaagcacc tggcgggcga gtacgaggcg gcgtcaccgg ccggcgggca ccacgtgtgg
     1741 gtcacgctca agcgccccgt cgaggagcgg acgctgtacg ccgaggcggt ccgccacggc
     1801 atgtcgttca cccccggcgg cgcggtcacc gccgagcgcc accagcgcac cagcctgcgg
     1861 ctgtcgttct cgctgctcga gcccgacgag ctcgaggagg gcgtgcgccg cctggcccgg
     1921 gcgctgcgcg aggtccgccg ccgcgaccgc acacgcgtgg ccgcccctct gagctgactg
     1981 gcagactgcc gccccgatgc tcgaggggcg cgaggaggaa ctggcctgga tggccacgcg
     2041 gctggaggcg ctccggcggc accggccgtt cgcggcgctg atccgcggtg aggccggggt
     2101 cggcaagagc tcgctgatcg acgcggcggt gggtcacgcc cggggcatcc gcgtgctgcg
     2161 cgctcgcggc tacgagtccg accgcgacat cccgttcgcc ggcctgctgg agctggcctc
     2221 cccgttgctg gagctgcgcg accggctgcc ggaggcccag cgggaggccg tcgaagccgc
     2281 gctcgcgctg aggcaggcgg gcccccacga ccgcttcgcc gtgccggcgg ggcttctggg
     2341 cctgctgtcc cacgcggccg aggacgagcc ggtggtggtc gtggtggacg acgtgcactg
     2401 gctggacgac gcgtcccgcg gcgcgctcct gttcgtggcc cggcggctgg cgggcgagcc
     2461 gatcggcgtg ttgctgggcc tgcgcgacgg cgtggcgccg gcgctggact tcagcggcat
     2521 cgaggagctg gcgctgaggg gcctgcctgt ggacagcgcc ctccgcgtac tgcacggggc
     2581 tgcgcccgac ctgcccgagc gcgtggcccg cacgctggtg gacgccaccg cggggaaccc
     2641 cctggccctg ctggagatcc ccgcggcgct gaccgcagag cagcgcgcgg ggacggacgc
     2701 cctgaccgac cccctgccca ccaccaccgc tctggagggc gccttc
//
LOCUS       DASUPW010000368         2136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_777268, whole genome shotgun sequence.
ACCESSION   DASUPW010000368 DASUPW010000000
VERSION     DASUPW010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777268"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VFY44_08265"
     CDS             <1..349
                     /locus_tag="VFY44_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910474.1"
                     /translation="EGNEAVMLCERGIRTFESSYRFTLDLMAVPVLKDLTHLPVIVDP
                     SHAAGRRDLVESMSMAAAAAGADGLIVEVHPDPDHAVCDGPQQLKADGFADYLARVER
                     VAEVAGKLPAAAV"
     gene            363..1454
                     /locus_tag="VFY44_08270"
     CDS             363..1454
                     /locus_tag="VFY44_08270"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF009395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX5910475.1"
                     /translation="MHVSQSKRLERVRDQRIARIAPLATPHRMLTELPLTPELEEVVI
                     SGRDQVSAVLDRADDRLLVVVGPCSVHDSKAATEYAGRLAAKAQELDEDLLVAMRVYF
                     EKPRTTTGWKGMINDPHLDDSGDVNTGLRRARKLLLEVLGLGLPVGCEFLDPITPQYI
                     SDTVAWGAIGARTTESQIHRQLGSGLSMPIGFKNRTDGNVQVAVDAVRAAAVSHAFAG
                     VDDEGTPAILHTRGNPDGHVILRGGRGAQNHGPDGVEQALEKLRAAGLPERVVVDASH
                     DNSGKDADRQPEVAAEIGRQLEDGQHAIVGVMLESFLVAGRQDLVPGRQLTYGQSITD
                     ACMAWDTTVDVLEALAASVRARREAAAPA"
     gene            1454..>2136
                     /locus_tag="VFY44_08275"
     CDS             1454..>2136
                     /locus_tag="VFY44_08275"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX5910476.1"
                     /translation="MRIAVLGAGLIGGSIGQAARQKLDDVEVVGHGRSAERLERALAA
                     GAIDRAAGSLPEALEGAGVCFCCAPVGALTGQVRDALAVAPADCVVTDVGSVKRTLVE
                     TIEDERFVGGHPIAGAETSGVEHARPDLFDGAVWYLTPTAASSGVLYERLHRLIVALG
                     ARPAAIDATTHDSLLAVVSHLPHVLANVLVAQAGSHSELPRIGPSFRDATRVAGANTG
                     IWTDIYLANR"
BASE COUNT          293 a          800 c          779 g          264 t
ORIGIN      
        1 ggagggcaac gaggcggtga tgctctgcga gcgcgggatc cgcacgttcg agtcgagcta
       61 ccggttcacg ctcgacctga tggcggtgcc ggtgctcaag gacctgaccc acctgccggt
      121 gatcgtcgac cccagccacg cggccggccg ccgcgatctg gtcgagtcga tgtccatggc
      181 cgcggcggcc gcaggcgccg acggcctgat cgtggaggtg caccccgacc ccgaccacgc
      241 cgtgtgcgac ggcccccagc agctgaaggc cgatggcttc gccgactacc tcgcccgcgt
      301 ggagcgcgtg gcggaggtcg cgggcaagct cccggccgcg gccgtctgac aggctcaggg
      361 acgtgcacgt gagccagtcc aagcgcctcg aacgggtccg cgatcagcgc atcgcccgga
      421 tcgcgccgct ggccacgccg caccggatgc tcaccgagct gccgctgacc cccgagctgg
      481 aggaggtcgt gatcagcggc cgcgaccagg tgtcggccgt gctcgaccga gccgacgacc
      541 gcctgctggt ggtcgtgggc ccgtgcagcg tgcacgactc caaggccgcg accgagtacg
      601 ccgggcggct ggccgccaag gcccaggagc tggacgagga cctgctggtg gccatgcgcg
      661 tgtacttcga gaagcccagg accaccaccg ggtggaaggg catgatcaac gacccccacc
      721 tggacgactc cggcgacgtg aacaccggcc tgcgccgcgc gcgcaagctg ctgctcgagg
      781 tgctgggcct gggcctgccg gtgggctgcg agttcctgga cccgatcacc ccgcagtaca
      841 tctccgacac cgtcgcctgg ggcgccatcg gcgcgcgcac caccgagagc cagatccatc
      901 gccagctggg ctcgggcctg tcgatgccga tcggcttcaa gaaccgcacc gacggcaacg
      961 tgcaggtggc ggtcgacgcg gtgcgcgccg ccgcggtgtc gcacgccttc gccggcgtcg
     1021 acgacgaggg caccccggcc atcctgcaca cgcgcggcaa ccccgacggc cacgtgatcc
     1081 tgcgcggcgg ccgcggcgcc cagaaccacg gccccgacgg ggtcgagcag gcgctggaga
     1141 agctgcgcgc cgccgggctg cccgagcgcg tggtcgtgga cgcctcacac gacaactcgg
     1201 gcaaggacgc cgaccgccag cccgaggtcg cggccgagat cgggcgccag ctcgaggacg
     1261 gccagcacgc catcgtcggc gtgatgctgg agtcattcct ggtcgccggc cgccaggacc
     1321 tggtgcccgg gcgccagctg acctatggcc agtccatcac cgacgcctgc atggcctggg
     1381 acaccaccgt cgacgtgctc gaggccctgg cggcctcggt ccgcgcgcgg cgcgaggccg
     1441 ctgccccggc ctgatgcgga tcgcggtcct gggcgccggc ctgatcggcg gctcgatcgg
     1501 ccaggcggca cgccagaagc tggacgacgt ggaggtcgtg ggccacggcc gctcggccga
     1561 gcggctggag cgggcgctgg ccgccggggc catcgaccgc gccgccggct cgctgcccga
     1621 ggcgctggag ggggccggcg tctgtttctg ctgcgccccg gtcggcgccc tgaccggcca
     1681 ggtgcgcgat gcgctggccg tggcgcccgc ggactgcgtg gtcaccgacg tcggctcggt
     1741 caagcggacg ctggtcgaga cgatcgagga cgagcggttc gtgggcggac acccgatcgc
     1801 gggcgccgag acctccggcg tggagcacgc ccgccccgac ctgttcgacg gcgcggtctg
     1861 gtacctgacg ccgaccgccg cctcgtccgg cgtgctctac gagcgcctgc accgcctgat
     1921 cgtggccctg ggggcccggc cggcggccat cgacgccacc acgcacgaca gcctcctggc
     1981 cgtggtcagc cacctgccgc acgtgctggc caacgtgctg gtggcccagg ccggctcgca
     2041 cagcgagctg ccccggatcg ggcccagctt ccgcgacgcc acccgcgtgg ccggcgccaa
     2101 cacgggcatc tggacggaca tctacctggc caaccg
//
LOCUS       DASUPW010000369         7492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_363029, whole genome shotgun sequence.
ACCESSION   DASUPW010000369 DASUPW010000000
VERSION     DASUPW010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7492
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363029"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VFY44_08280"
     CDS             <1..513
                     /locus_tag="VFY44_08280"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5910477.1"
                     /translation="LTQHTKFSGQDLSFYDQETSTRYVPHVIEPAAGADRATLAFMVD
                     AYDAEEVEGRERVLLRLHPKLAPVKAAVLPLLNKEGQPEKARAIYEELRERMSAEFDT
                     GGSIGKRYRRQDEIGTPWGITVDHQTMEDDTVTLRDRDSLEQTRIAADQVADELTRRL
                     AADWVSPKLA"
     gene            complement(787..1344)
                     /locus_tag="VFY44_08285"
     CDS             complement(787..1344)
                     /locus_tag="VFY44_08285"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEX5910478.1"
                     /translation="MDSAKSVTLPSKRTLQVTGVIVFLIGAAAVLVPAVASVTITLLV
                     GWLLGFAGAMAVISAIMHPGDHRGLRALWGLVALGVGAYFVIFAPEGTKTLTLVLGIN
                     FLAMGAIRMGAWMALRGTAGAAAMGFNGLVSVILGVIILIDYPDSAAWAIGLLVGIEL
                     MFFGIALFSGGRTVDHTAPTGARTV"
     gene            1420..2283
                     /locus_tag="VFY44_08290"
     CDS             1420..2283
                     /locus_tag="VFY44_08290"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5910479.1"
                     /translation="MQPLFEHRLYLAGYRTRALELEGDGPPLLLLHGFSDSADTWRIL
                     LDKLARRGRRAVAVDLPGFGTCDVLSDHEPILVQHRRFAAAAVEWMAPEGGAVVVGNS
                     LGGLVALLLAEDDAHGLAGIVPVAPAGLDMARWFTVIERDPLLRTLLAAPVPIPRVAL
                     QRVVAEVYKRLAFHRPRDIDPRVAATFASHFADRATTGRLLGTGRRLLPELRDCLTPE
                     RIDCPLLMVWGERDVMVFPTGAQRVLDAVEGSRLVTMERCGHSPQIERPDQLAELVLE
                     FPASLADATTS"
     gene            2309..3367
                     /locus_tag="VFY44_08295"
     CDS             2309..3367
                     /locus_tag="VFY44_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910480.1"
                     /translation="MTGWDHLLGGGVADTTPTPSSIIHEAPQCTVHRYRSRRRARADR
                     APVLLVPPLAAPASCFDLHRGCSLAQHLTAMGYPVYLVDYGPIGFHDRALGLEHWVED
                     VIPTAMRVASADAGGVPVQPVGWCLGGILTLLAVAADRSLPVASVAMVASPFDFEQMR
                     LLGPIRQLGRLTGGALVGALYRALGGAPAPLVSRAFQLTSLDRYLTKPVFVATHLHDR
                     ETIAHMEAVDAYMAHMSAYPGRTFGQLYHAFFRVNELAGGKVELRRGTVIDLSDVTVP
                     VLSVAGAVDVLAPEAAVRHVGALLADVRLETAPGGHLGVLTGRSARDTTWEYLDEFLG
                     EHDPVVSGRATPVEARRA"
     gene            complement(3364..4482)
                     /locus_tag="VFY44_08300"
     CDS             complement(3364..4482)
                     /locus_tag="VFY44_08300"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5910481.1"
                     /translation="MGIESLAELTLESMKHALPVLREDPDLLQAARSSSAANIALVTE
                     LIARRETLADLESPPQATAFARELARRNVPVADLARAYRVAEIALWRWAVAEVRARVP
                     ADGLADALEGASEAVFETGDAFSTLVTERYALERERWMRSADAVRSATVQELLDGAPT
                     DPLSASRRLRYELRQRHAAFVVWGGADGSVPETAAAGVGGTRALLVPMGPDLIAGWAP
                     PDAVNPHAAEGAAHVALGTPREGLAGFRSSHLEAMEARRVTRLLGVGVDPIHYDDVAL
                     LALLTKDLDQARAFARRTLGPLAGDDAGTRRLADTLLVLLEEEGRPRRAAARLGVHEN
                     TVAKRLRAIRELLGEDPAERPADMLAALLILRAGRAEG"
     gene            4652..6010
                     /locus_tag="VFY44_08305"
     CDS             4652..6010
                     /locus_tag="VFY44_08305"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEX5910482.1"
                     /translation="MADRLSSSRFGRAARIGGLAAGAAGRTLSGRATTVGRSEEAKAE
                     ANRKAVLETAERMVTVLGSMRGAAMKIGQTLSVIDLGLADDELSEEFQAKLARLQHMA
                     PTVAFADMRKVIEDDLGARIGDAFAEFDEEPIAAASIGQVYKARLDDGRDVAVKVQYP
                     GIKKTVESDLKNLGLVLKAGSRMVPGLASREVAEEITERIVEELDYELEAANHRALAR
                     EYRGHPFIAVPEVVTDMCTERVMVTEFVPARRFADVAEEPDEVRDRYGEILFRFYVNG
                     PFRHRLLSGDPHPGNSLFPADGSVAFLDFGFFKRQSARQVEDQREVLKAVYAQDADRL
                     FELSREQGVIGDDRSLVQPVMEKYRAATWWFLEDREITLTPADVTRIVWEHADMRKGF
                     GDVGLPAEQVTTLRAFGLTLGILAQLRATNNWFRIGREVVFGDEPATELGRAEAEWLA
                     GR"
     gene            6032..7174
                     /locus_tag="VFY44_08310"
     CDS             6032..7174
                     /locus_tag="VFY44_08310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5910483.1"
                     /translation="MSLELTDEQSDFVDAIRDFATRECGTREQRDALTGGGKHPHNQD
                     LYGQIAELGWLGVAIPEEYGGTGGGMVDLCLLLEESARGLIPMGGFGVSMIVAGAVEK
                     FGTEDQKREILEGIVAGRVESIAMSEPGAGSDVGALQCRAQRQNGGYVVEGQKTWISE
                     AHLSDHVLLVCRTDRDGAKHEGLTMLSVPNDAEGMEIRPIETMGGEVVNDVFFTDCHV
                     PAERLLGEEGNAWMQLMSGLNVERLILAALMLGVAQRAFDDTLSYVKEREQFGRPIGS
                     FQALKHRLADVATEIECCRLLTYDVAQKVDANPGAMFPREASMAKLKVTETAKRMALE
                     GMQMMGGYGYATEYDMEGHVRATLVSTIYGGTSEIQRDIIGRTYGL"
     gene            7171..>7492
                     /locus_tag="VFY44_08315"
     CDS             7171..>7492
                     /locus_tag="VFY44_08315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX5910484.1"
                     /translation="MSQETPPELSYVLGHFGLAAGNANVIMQLARLPVGHGVARSTVD
                     SGRVDKHPIKRLRTTASYLAVSMMGTEAERRAMRHEVNRAHAAVHSGPSDPVPYNAFD
                     QDLQL"
BASE COUNT         1065 a         2725 c         2740 g          962 t
ORIGIN      
        1 ctcacccagc acacgaagtt ctccggccag gacctgtcgt tctacgacca ggagacgagc
       61 acccggtacg tgccgcacgt gatcgagccg gcggcgggcg ccgaccgcgc gacgctcgcg
      121 ttcatggtcg acgcctacga cgccgaggag gtcgagggcc gcgagcgcgt gctgcttcgc
      181 ctccacccca agctcgcccc cgtcaaggcc gccgtgctgc cgctgctgaa caaggagggc
      241 cagcccgaga aggcgcgcgc catctacgag gagctgcgcg agcggatgtc ggccgagttc
      301 gacaccggcg gatcgatcgg caagcgctac cggcgccagg acgagatcgg caccccgtgg
      361 ggcatcaccg tcgaccacca gacgatggag gacgacaccg tcacgctgcg cgaccgcgac
      421 tcgctcgagc agacgcgcat cgccgccgac caggtggccg acgagctgac gcggcgcctg
      481 gccgccgact gggtctcgcc gaagctggcc tgatcgtcct gccggcgtcg ttcccggcgg
      541 gtccggcgaa acggcggccg ggtagggcag cggccacctg tcacgttcgt cgcgctcccg
      601 tcacaccggg cgcgacccca cagccccacc ctcgcgccgc gcggtgcatt tctcccgctg
      661 agcgggagca atccgccgcg cggccgcagc cgtgtcacgg gagcgcgcga acggtcacag
      721 cctcttcgcc ctacccggcc caggccgtta cgtcgatccc gcaggggcgg gcgacggcag
      781 gacgcctcag accgtccgcg cccccgtcgg cgccgtgtgg tcgacggtgc gcccgccgga
      841 gaacagggcg atgccgaaga acatcagctc gatgcccacc agcaggccga tcgcccaggc
      901 ggccgagtcg gggtagtcga tcaggatgat cacgccgagg atgacgctga ccaggccgtt
      961 gaagcccatg gcggcggcgc ccgccgtccc ccggagggcc atccaggcgc ccatccggat
     1021 cgcgcccatg gccaggaagt tgatgcccag gacaagggtc agggtcttgg tgccctcggg
     1081 cgcgaagatc acgaagtagg cgccgacgcc gagcgccacc aggccccaga gcgcccgcag
     1141 gccgcggtga tcgccgggat gcatgatcgc gctgatcacg gccatcgcgc cggcgaagcc
     1201 cagcagccag ccgaccagga gggtgatcgt gaccgaggcg acggcgggca cgagcaccgc
     1261 cgcggcgccg atcaggaaga cgatcactcc cgtgacctga agcgtgcgct tggagggcaa
     1321 cgtgaccgac ttcgcggagt ccatggggcc aagcatcgcc cgcccggcgg acgaccgcaa
     1381 gcgagagtcc gggatcaccc gtacgctggt gccccagtta tgcagcccct cttcgagcac
     1441 cggctgtacc tggcggggta ccggacgcgg gccctggagc tggagggcga cgggccgccg
     1501 ctgctgctgc ttcacggctt ctccgacagc gccgacacct ggcggatcct gctggacaag
     1561 ctggcccgcc gtggtcgccg cgccgtggcc gtggacctgc cgggcttcgg cacctgcgac
     1621 gtgctcagcg accacgagcc gatcctggtc cagcaccgcc ggttcgcggc cgccgccgtc
     1681 gagtggatgg cgcccgaggg gggcgccgtg gtggtgggca actcgctggg cgggctggta
     1741 gcgctgctgc tggccgagga cgacgcccac ggcctggcgg gcatcgtgcc ggtggccccc
     1801 gcgggcctgg acatggcgcg ctggttcacg gtgatcgagc gcgacccgct gctgcggacg
     1861 ctgctggcgg cgccggtgcc gatcccgcgc gtggcgctcc agcgggtggt ggccgaggtc
     1921 tacaagcggc tggccttcca ccggccgcgg gacatcgacc cgcgcgtggc ggccacgttc
     1981 gcgtcccact tcgccgaccg cgccaccacc ggccggctgc tggggaccgg ccggcggctg
     2041 ctgcccgagc tgcgcgactg cctgaccccg gagaggatcg actgccccct gctgatggtc
     2101 tggggcgagc gcgacgtgat ggtcttcccg accggcgcac agcgggtgct ggacgcggtc
     2161 gagggctcgc ggctggtgac gatggagcgc tgcggccact cgccccagat cgagaggccc
     2221 gaccaactcg ccgagctggt tctagagttc ccggcctcac tggccgatgc gactacttcc
     2281 tgacccagag acgatcgtcg actcggccgt gaccggctgg gaccacctgc tgggcggcgg
     2341 ggtggccgac acgacgccca cgccctcgtc gatcatccac gaggcgccgc agtgcaccgt
     2401 gcaccgctac cgctcgcgcc gccgcgcccg cgccgaccgc gcgccggtgc tgctggtgcc
     2461 gccgctggcc gcccccgcct cctgcttcga cctgcaccgc ggctgcagcc tggcccagca
     2521 cctgacggcc atgggctacc ccgtgtacct ggtggactac ggcccgatcg gcttccacga
     2581 ccgggccctg gggctcgagc actgggtcga ggacgtgatc cccacggcga tgcgggtggc
     2641 ctcggccgac gccggcggcg tgccggtcca gcccgtgggc tggtgcctgg gcggcatcct
     2701 gacgctgctg gccgtcgcgg ccgaccgcag ccttccggtg gcgtcggtgg ccatggtggc
     2761 cagcccgttc gacttcgagc agatgcggct gctgggcccc atccgccagc tgggccggct
     2821 gaccggcggc gcgctggtcg gcgccctgta ccgcgcgctg ggcggggccc cggcgccgct
     2881 ggtcagccgc gcgttccagc tgacgtcgct ggaccggtac ctgaccaagc cggtgttcgt
     2941 ggccacccac ctgcacgacc gcgagacgat cgcccacatg gaggcggtgg acgcctacat
     3001 ggcccacatg tcggcctacc ccggccgcac cttcggccag ctctaccacg cgttcttccg
     3061 cgtgaacgag ctggccggcg ggaaggtcga gctgcgcagg ggcacggtga tcgacctgtc
     3121 cgacgtgacc gtgccggtgc tcagcgtggc cggggccgtc gacgtgctgg ccccggaggc
     3181 ggcggtccgg cacgtgggcg cgctgctggc ggacgtgcgg ctggagaccg ccccgggcgg
     3241 ccacctcggg gtgctgaccg gccgctcggc ccgcgacacc acgtgggagt acctggacga
     3301 gttcctgggc gagcacgatc cggtggtgtc cgggcgcgcg acaccggtcg aagcccgccg
     3361 ggcctagccc tcggcgcgcc ccgcccgcag gatcagcaac gccgcgagca tgtccgcggg
     3421 acgctcggcg ggatcctcgc ccagcagctc ccggatcgcc cgcagccgct tggccacggt
     3481 gttctcgtgc acgcccaggc gggcggccgc gcgcctgggc cgtccctcct cctccagcag
     3541 caccagcagc gtgtcggcca gccgccgggt gccggcgtcg tcgccggcca gcgggcccag
     3601 cgtgcgccgg gcgaacgcgc gcgcctggtc gaggtccttg gtcagcagcg ccagcagggc
     3661 cacgtcgtcg tagtggatcg ggtcgacgcc gacgcccagc agccgcgtga cccggcgggc
     3721 ctccatcgcc tccagatggc tggagcggaa gccggcgagc ccctcgcgcg gcgtgcccag
     3781 ggccacgtgg gcggcgccct cggcggcgtg cgggttcacg gcgtcggggg gcgcccagcc
     3841 ggcgatcagg tcggggccca tcggcaccag gagcgcccgc gtgcccccca cgcccgccgc
     3901 cgcggtctcg ggcaccgacc cgtcggcgcc gccccagacg acgaacgcgg cgtggcgctg
     3961 gcgcagctcg taccgcagcc gccggctggc cgagagcggg tccgtggggg cgccgtcgag
     4021 cagctcctgc accgtggcgc tgcgcaccgc gtcggcgctg cgcatccacc gctcgcgctc
     4081 gagcgcgtag cgctcggtga cgagcgtgct gaaggcgtcg ccggtctcga acaccgcctc
     4141 cgacgccccc tccagcgcgt cggccaggcc gtcggcgggc acccgcgccc gcacctccgc
     4201 gaccgcccac cgccagagcg cgatctcggc cacgcggtag gcccgggcca ggtcggccac
     4261 cggaacgttg cgccgcgcca gctcgcgcgc gaacgccgtg gcctggggcg gtgactcgag
     4321 gtcggcgagc gtctcgcggc gtgcgatcag ctcggtgacc agcgcgatgt tggccgccga
     4381 gctgctgcgc gcggcctgga gcaggtcggg gtcttcgcgc aacaccggga gcgcgtgctt
     4441 catcgactcc agcgtgagct ccgcgagcga ctcgattccc acctctcgcc cggcctctcc
     4501 gagcgcttga cgccagcctg gaggggttgc catgggcgac atcctatgac gccttcgccc
     4561 gccccggtgt gcccgcgcca cacccgatcc cttccaggcg gcgacgccac cacagccccg
     4621 gcggtcgcgc agcggagagg atggtccccc gatggccgac cgcctgagca gctcccgctt
     4681 cggacgcgcc gcgcggatcg gcggcctggc cgcgggcgcg gccggccgga ccctgagcgg
     4741 ccgtgccacc acggtcgggc ggtcggagga ggcgaaggcc gaggccaacc gcaaggccgt
     4801 gctggagacc gccgagcgga tggtcacggt gctgggctcg atgcggggcg cggccatgaa
     4861 gatcggccag acgctgtcgg tgatcgacct ggggctggcg gacgacgagc tgtccgagga
     4921 gttccaggcc aagctggccc ggctccagca catggcgccg accgtggcct tcgccgacat
     4981 gcgcaaggtc atcgaggacg acctcggcgc gcggatcggc gacgcgttcg ccgagttcga
     5041 cgaggagccg atcgccgcgg cgtcgatcgg ccaggtgtac aaggcccgcc tggacgacgg
     5101 gcgcgacgtg gcggtcaagg tccagtaccc cgggatcaag aagaccgtgg agtccgacct
     5161 caagaacctc gggctggtgc tcaaggccgg ttcgcggatg gtgcccgggc tggccagccg
     5221 cgaagtggcc gaggagatca ccgagcgcat cgtcgaggag ctggactacg agctcgaggc
     5281 ggccaaccac cgcgcgctgg cgcgggagta ccgcggccac cccttcatcg ccgtgcccga
     5341 ggtggtcacg gacatgtgca ccgagcgcgt gatggtcacc gagttcgtgc ccgcacgccg
     5401 gttcgccgac gtggccgagg agcccgacga ggtgcgcgac cgctacggcg agatcctgtt
     5461 ccggttctac gtcaacggcc cgtttcgcca ccgcctgctc agcggcgacc cgcatcccgg
     5521 caactccctg ttcccggccg acggcagcgt ggccttcctg gacttcggtt tcttcaagcg
     5581 ccagagcgcc cggcaggtcg aggaccagcg cgaggtcctg aaggcggtct acgcccagga
     5641 cgccgaccgg ctgttcgagc tctcgcgcga gcagggggtg atcggcgacg accggtcgct
     5701 ggtgcagccg gtgatggaga agtaccgcgc cgccacgtgg tggttcctgg aggaccgcga
     5761 gatcacgctg acccccgccg acgtgacgcg gatcgtgtgg gagcacgccg acatgcgcaa
     5821 ggggttcggc gacgtggggc tgcccgccga gcaggtgacc acgctgcgcg ccttcggcct
     5881 gacgctgggc atcctggccc agctacgggc caccaacaac tggttccgga tcggccgcga
     5941 ggtcgtcttc ggcgacgagc cggccaccga gctgggacgc gccgaggccg agtggctggc
     6001 cgggcgctga caccgacgac ccgggaggga catgagcctc gagctgaccg atgagcagag
     6061 cgacttcgtg gacgcgatcc gcgacttcgc cacacgcgag tgcgggaccc gcgagcagcg
     6121 cgacgcgctg accggcgggg gcaagcaccc ccacaaccag gacctgtacg ggcagatcgc
     6181 cgagctgggc tggctggggg tcgcgatccc cgaggagtac ggcggcaccg gcggcggcat
     6241 ggtcgacctg tgcctgctgc tggaggagag cgcccgcggg ctgatcccca tgggcggctt
     6301 cggcgtgtcg atgatcgtgg ccggcgccgt cgagaagttc ggcaccgagg accagaagcg
     6361 cgagatcctg gagggcatcg tggccggcag ggtcgagtcg atcgccatga gcgagcccgg
     6421 cgcgggctcc gacgtgggcg ccctccagtg ccgcgcacaa cgccagaacg gcggctacgt
     6481 ggtcgagggc cagaagacct ggatcagcga ggcgcacctg tccgaccacg tgctgctggt
     6541 gtgccgcacc gaccgcgacg gggccaagca cgaggggctg accatgctca gcgtccccaa
     6601 cgacgccgag gggatggaga tccgcccgat cgagaccatg ggcggcgagg tggtcaacga
     6661 cgtgttcttc accgactgcc acgtgcccgc cgagcggctg cttggcgagg aggggaacgc
     6721 gtggatgcag ctgatgtcgg gcctgaacgt ggagcgcctg atcctggcgg cgctgatgct
     6781 gggcgtggcc cagcgcgcgt tcgacgacac gctgtcctac gtcaaggaac gggagcagtt
     6841 cggccgcccg atcggatcgt tccaggccct caagcaccgg ctggccgacg tggccaccga
     6901 gatcgagtgc tgccggctgc tgacatacga cgtcgcccag aaggtggacg ccaaccccgg
     6961 ggcgatgttc ccgcgcgagg cgtcgatggc caagctgaag gtcaccgaga ccgccaagcg
     7021 gatggcgctg gagggcatgc agatgatggg cggctacggc tacgccaccg agtacgacat
     7081 ggagggccac gtgcgggcca cgctcgtctc gaccatctac ggcggcacca gcgagatcca
     7141 gcgcgacatc atcgggcgca cgtacggcct gtgagccagg agaccccgcc cgagctgtcg
     7201 tacgtgcttg gccacttcgg gctcgcggcc ggcaacgcca acgtgatcat gcagctggcc
     7261 cggctgcccg tcgggcacgg cgtggcccgc agcacggtcg acagcggccg tgtggacaag
     7321 cacccgatca agcggctgcg gaccaccgcc tcgtacctgg cggtgtcgat gatgggcacc
     7381 gaggccgagc gccgggccat gcgccacgag gtcaaccggg cgcacgccgc cgtgcactcg
     7441 gggccctccg acccggtccc ctacaacgcg ttcgaccagg acctccagct gt
//
LOCUS       DASUPW010000370         2835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1194263, whole genome shotgun sequence.
ACCESSION   DASUPW010000370 DASUPW010000000
VERSION     DASUPW010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194263"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFY44_08320"
     CDS             <1..262
                     /locus_tag="VFY44_08320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5910485.1"
                     /translation="MQNASKHAPDARSVRVALSERDGLAFEVRDDGAGFDPDVATPGV
                     GLTNMHDRLAAVGGAVRITSAPGAGTVVAGTVPLGATATSRG"
     gene            266..1036
                     /locus_tag="VFY44_08325"
     CDS             266..1036
                     /locus_tag="VFY44_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910486.1"
                     /translation="MASAPSAWPAIPEEDLPELMALIEDADSVELKLTVPEPDQRPTF
                     ESLHIDPLEAQVRLVHFYDTPDLQLDRAGVVVRARRVQRKGDDSVVKLRPVEPDHLPR
                     KVRRSENFGVEVDAMPGGFVCSGTMKRALPAGAVQEVAAGERALRKLFTKEQRRFYVD
                     HAPAGIGLDDLVLLGPIFVLKLKFSPQRLRRRMVAELWLYPDSSRVLELSTKCAPGEA
                     FQVAAQTRALLADAGVSLTGKQQTKTSKALRYFAGALG"
     gene            complement(1033..2220)
                     /locus_tag="VFY44_08330"
     CDS             complement(1033..2220)
                     /locus_tag="VFY44_08330"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX5910487.1"
                     /translation="MDDLAVLAVVLLGYGLVSRRLEAGVVTGPLAFVAAGVVAGPHVL
                     GLTDGELLEGAGLILAEAALAFLLFSDAAGVDLRRLRAEEGLPARLLGIGMPLTIALG
                     VGAGALLLGDMEFWEAAIVAAVLAPTDAALGQAVVTSERVPRRIRQSLNVEAGLNDGL
                     SVPFVALFAALAVEEADPSAQHWLTFAAEQIGYGALIGAALGAVGGWLLARASARGLM
                     TTAFEHLAVLALAVLAFTLADQAGGNGFIAAFAGGLGAGRMGLTGHRMLEFEEEEGQL
                     LSLAVFFFFGVAAVDLVEPAGWEVVAYAALSLTVIRMVPVAVAVTGTGLRPRAVAFMG
                     WFGPRGLASIVLVLTVAEEHPDLPSLDVVLATVAVTVLASVVLHGLTAQPFIRAYARE
                     EDD"
     gene            2378..2827
                     /locus_tag="VFY44_08335"
     CDS             2378..2827
                     /locus_tag="VFY44_08335"
                     /inference="COORDINATES: protein motif:HMM:NF039728.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HEX5910488.1"
                     /translation="MESHEEDEIGPIDVIVIGYPVGAPMTGEAMPIFLDLVDRGIIRV
                     LDALFVVKAEDGTVSGFDATDLDVKDVGDFEALAGASSGLLGGDDVQTAGEALDPGSA
                     AVMIVYENRWAAPFAAAVRRNGGVLIASDRIPVQDVIDALDAAEAAA"
BASE COUNT          421 a         1057 c         1025 g          332 t
ORIGIN      
        1 gatgcagaac gcctccaagc acgcgcccga tgcccggtcg gtcagggtcg ccttgagcga
       61 gcgcgacggg ctggcgttcg aggtccgcga cgacggcgcc ggcttcgacc cggatgtcgc
      121 gacgccggga gtggggctca cgaacatgca cgaccgcctg gccgccgtcg gcggtgcggt
      181 gcggatcacg tcggcgccgg gcgccggcac cgtcgtggcc ggcacggtcc cgctcggcgc
      241 gacggcgacc tcgcggggct gagcgatggc ctccgctccc agcgcctggc ccgcgatccc
      301 cgaggaggac ctgccggagc tgatggcgct gatcgaggac gccgacagcg tggagctcaa
      361 gctgaccgtc cccgagcccg accagcgccc caccttcgag tccctgcaca tcgacccgct
      421 ggaggcccag gtgcgcctgg tgcacttcta cgacacgccg gacctccagc tggaccgggc
      481 cggcgtggtg gtgcgtgcgc gccgcgtgca gaggaagggc gacgactcgg tggtcaagct
      541 ccgcccggtg gagccggacc acctgccgcg caaggtccgc aggtcggaga acttcggcgt
      601 ggaggtggac gccatgccgg gcgggttcgt ctgctcgggc acgatgaagc gcgccctgcc
      661 cgcgggcgcc gtgcaggagg tggcggcggg cgagcgggcg ctgcgcaagc tgttcacgaa
      721 ggagcagcgg cgcttctacg tcgaccacgc gcccgccggc atcggactgg acgacctggt
      781 cctgctgggg ccgatcttcg tgctcaagct gaagttctcg ccgcagcggc tgcggcgccg
      841 gatggtcgcc gagctgtggc tctaccccga ctccagccgg gtgctcgagc tgtccaccaa
      901 gtgcgcgccc ggcgaggcct tccaggtggc ggcccagacc cgtgcgctgc tggcggacgc
      961 cggggtgagc ctcaccggca agcagcagac gaagaccagc aaggccctca ggtacttcgc
     1021 cggcgcgctg ggctagtcgt cctcctcgcg cgcgtaggcg cggatgaacg gctgcgcggt
     1081 cagcccgtgg agcaccacgc tggccagcac cgtgacggcc accgtggcca gcacgacgtc
     1141 cagcgacggc aggtccgggt gctcctcggc caccgtcagc accagcacga tcgacgccag
     1201 gccccggggg ccgaaccagc ccatgaaggc gaccgcgcgc gggcgcagac ccgtgccggt
     1261 cacggccacc gccacgggca ccatccggat caccgtcagg ctcagggcgg cgtaggccac
     1321 gacctcccac ccggcgggct cgaccaggtc cacggcggcg acgccgaaga agaagaagac
     1381 ggccaggctc agcagctggc cctcctcctc ctcgaactcg agcatccggt ggccggtgag
     1441 ccccatgcgc cccgcgccga gcccgccggc gaaggcggcg atgaacccgt tcccgccggc
     1501 ctggtcggcc agggtgaacg ccagcacggc cagggccagc acggccaggt gctcgaaggc
     1561 cgtggtcatc agcccgcggg ccgacgcgcg ggccaggagc cacccgccca cggcccccag
     1621 cgcggcgcct atcagcgcgc cgtagccgat ctgctcggcg gcgaaggtca gccagtgctg
     1681 cgccgagggg tccgcctcct ccacggccag cgcggcgaag agcgccacga agggcaccga
     1741 cagcccgtcg ttcaggccgg cctcgacgtt aaggctctgc cggatccgcc gcggcacgcg
     1801 ctcgctggtc accaccgcct ggccgagggc cgcatcggtg ggggcgagga cggcggcgac
     1861 gatggccgcc tcccagaact ccatgtcgcc cagcagcagg gccccggcgc ccacgcccag
     1921 ggcgatggtc agcggcatcc ctatccccag cagccgcgcg gggaggccct cctcggcccg
     1981 gagccggcgc aggtccacgc cggcggcgtc ggagaacagc aggaaggcca gcgccgcctc
     2041 ggccaggatc agcccggcgc cctcgagcag ctccccgtcg gtgagcccca gcacgtgggg
     2101 gcccgcgacc acgccggccg ccacgaacgc cagcggcccg gtgaccaccc cggcctccag
     2161 gcggcgggac accaggccgt agcccaacag cacgacggcc aggaccgcca ggtcgtccac
     2221 gtccgcgagt ctcggcccgc gcgcacccgc gggcaaggtt ggcggtatgc ctccaacggt
     2281 ggtcctggcc ctactcgcgt ggtcgtgcgc gcaccctcgc cgccggggcg ggcggaccgt
     2341 agcttcccct cgactcacag aaaccgagga ggaacggatg gaatcgcacg aagaggatga
     2401 gatcggcccg atcgacgtga tcgtgatcgg ctatccggtg ggcgcgccca tgaccggcga
     2461 ggcgatgccg atcttcctcg acctggtcga ccgcgggatc atccgcgtgc tggacgccct
     2521 gttcgttgtc aaggccgagg acggcaccgt ctccggcttc gacgccaccg acctggacgt
     2581 caaggacgtg ggcgatttcg aggccctggc cggtgcctcg tccggcctgc tgggcggcga
     2641 tgacgtccag accgccggag aggcgctcga tcccggatcc gcggcggtca tgatcgtcta
     2701 cgagaaccgc tgggcggcgc cgttcgccgc cgcggtgcgc cgcaacggcg gggtgctgat
     2761 cgccagcgac cggatccccg tgcaggacgt gatcgacgcc ctcgacgccg ccgaagcggc
     2821 ggcctgacga aagga
//
LOCUS       DASUPW010000371         3881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1331464, whole genome shotgun sequence.
ACCESSION   DASUPW010000371 DASUPW010000000
VERSION     DASUPW010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331464"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="VFY44_08340"
     CDS             <1..1099
                     /locus_tag="VFY44_08340"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX5910489.1"
                     /translation="GVLPTPGAALLTRRYGFDLAVVVSASHNPYTDNGIKIFGPDGMK
                     ISDAFEQGIEAALAGEPPPAAPGRVRELHGAPGDYLRELELRFSGLDLSGLKVLLDCA
                     NGATYRVAPEIFRRLGADVDAVAAAPDGRNINDGVGSTHIEGLGTQVVEGGYDAGFAF
                     DGDGDRVLAVDRTGAVVDGDELLALAALHLRGAGRLPGGGVAVTVMTNYGFHTAMEQA
                     GVEVAITPVGDRHVLAELLRRGWALGGEQSGHIIDTGFVPSGDGTASALLTLEALAGT
                     DLADRAGMQKLPQKLVNVTVAQRGVLDGATAVWAAIDAESAKLEGRGRVLVRASGTEP
                     LIRVMVEAPDQAECDDVTERLVTVVTQELGS"
     gene            1125..2951
                     /gene="glmS"
                     /locus_tag="VFY44_08345"
     CDS             1125..2951
                     /gene="glmS"
                     /locus_tag="VFY44_08345"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX5910490.1"
                     /translation="MCGIIGYTGSRPCQEILMAGLQALEYRGYDSAGLTLLQDGDAQT
                     TRAVGPLKALRERVNGAAPEAHSGIAHTRWATHGRVTEENCHPHDDASGRVHVVMNGI
                     VENWTDLKKELLADGARFTSETDTEVVAHLIASLYDGDIVAATREAFKRLKGHYAFVA
                     VHADEPDVLVGARNECPLVVGLGEGENYIASAIPAFLADTRRIQLIEDGDIVVVTPEG
                     VSFSDADGAPVEHDVEEVDWDEETAEKSGYETFMLKEIHEQPRAVAETVVDRLAHGRA
                     ELGDIGISDDELRGLRRVVIVACGTSYHAGLIGRYAIETWSRVPVEMDVASEFRYRDP
                     VIDDRDLVIGITQSGETLDTLAAMRLAREKGAKVMAVTNIMGSQATRDADGVLYTRAG
                     LEIGVAATKTFVSQVAAMYLFALKLAQVHDMLETERHRELVRDLRDLSHKVEELLATV
                     DEPVKRMAEHWKESGFFLYLGRHAGLAVALEGALKLKEISYVPTDAYAAGEMKHGPIA
                     LLDERTPVVVSATESPVLDKVLSNISEVTVRGAHVLAVATEGSTEVAEHAEEVVYVPK
                     TNWLLQPLLAVIPLQLLAYYIAQHKGLNVDQPRNLAKTVTVE"
     gene            2954..3301
                     /locus_tag="VFY44_08350"
     CDS             2954..3301
                     /locus_tag="VFY44_08350"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX5910491.1"
                     /translation="MIGLDLLEIERFEQVLERRPRIAERLFTDAEREYAAGKARPGQH
                     LAARFCAKEAVAKALALEGWSFTDVEVVATGGAPGVRLSGSAGARADQLGVEVRLSLT
                     HTETTAGAVALAL"
     gene            3298..>3881
                     /locus_tag="VFY44_08355"
     CDS             3298..>3881
                     /locus_tag="VFY44_08355"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEX5910492.1"
                     /translation="MTALPDWLDPLFEAQEMRDVDAWAIDTQGVPSLDLMERAGEGLA
                     RITAAAARRGPIRIVVGKGNNGGDGLVVARLLREEGREVDVLAVADLGGLEGDARANL
                     DRLPGDPPEDFSAEGLDGSGAVVDAMLGTGFSGEPREPIASAIAAINAQDAPVVACDV
                     PSGVGASTGRVEGQAVRATVTGTFHGPKVGLYVAP"
BASE COUNT          564 a         1375 c         1449 g          493 t
ORIGIN      
        1 cggcgtcctg cccactccgg gcgcggccct gctcacacgc cggtacggct tcgatctcgc
       61 ggtggtggtc tccgcctccc acaaccccta caccgacaac gggatcaaga tcttcggccc
      121 ggacgggatg aagatctccg acgccttcga gcagggcatc gaggctgcgc tcgccggcga
      181 gccgccgccg gcggcaccgg gccgagtgcg cgagctgcac ggtgcgcccg gggactacct
      241 gcgcgagctc gagctgcgct tcagcggcct cgacctgagc ggcctgaaag tcctgctgga
      301 ctgcgccaac ggcgccacct accgcgtggc gcccgagatc ttccgccgcc tgggcgccga
      361 cgtggacgcc gtggcggccg cccccgacgg gcgcaacatc aacgacggcg tcggctccac
      421 ccacatcgag gggctcggca cccaggtcgt ggagggcggc tacgacgcgg gcttcgcctt
      481 cgacggcgac ggcgaccggg tgctggccgt ggaccgcacc ggtgccgtgg tggacggcga
      541 cgagctcctg gccctggccg cgctgcacct gcgcggggcg ggccgcctgc ctggcggggg
      601 cgtggccgtg accgtgatga ccaactacgg cttccacacg gccatggagc aggccggggt
      661 ggaggtggcg atcacgccgg tgggcgaccg ccacgtgctg gccgagctgc tgcggcgcgg
      721 ctgggccctg ggcggcgagc agtcgggcca catcatcgac acgggcttcg tgccctcggg
      781 cgacggcacc gcctcggcgc tgctgaccct ggaggcgctg gccgggaccg acctggccga
      841 ccgcgccggc atgcagaagc tgccccagaa gctggtgaac gtcaccgtgg cccagcgggg
      901 cgtgctggac ggcgcgacgg ccgtctgggc cgccatcgac gccgagtcgg ccaagctgga
      961 gggccgcggc cgcgtgctgg tgcgcgcctc gggcaccgag cccctgatcc gcgtgatggt
     1021 cgaggccccc gaccaggccg agtgcgacga cgtgaccgag cgcctggtga cggtggtcac
     1081 gcaggaactc ggctcctgag cgcggagtac cctctcaacc tctcatgtgc ggcatcatcg
     1141 gatacaccgg atcgcgcccg tgccaggaga tcctcatggc cgggctccag gccctggagt
     1201 accgcggcta tgactccgcg ggcctgacgc tgctccagga cggggatgcg cagaccacgc
     1261 gcgccgtggg gccgctgaag gccctgcgcg agcgggtcaa cggcgccgcg cccgaggccc
     1321 acagcggcat cgcgcatacg cgctgggcca cccacggccg ggtgaccgag gagaactgcc
     1381 acccccacga cgacgcctcg ggccgcgtgc acgtggtcat gaacggcatc gtcgagaact
     1441 ggaccgacct gaagaaggag ctgctggccg acggcgcgcg gttcacctcc gagaccgaca
     1501 ccgaggtggt cgcccacctg atcgcgtcgc tgtacgacgg cgacatcgtg gccgccaccc
     1561 gcgaggcgtt caagcgcctg aaggggcact acgcgttcgt ggccgtgcac gccgacgagc
     1621 ccgacgtgct ggtgggcgcg cgcaacgagt gcccgctggt cgtgggcctg ggcgagggcg
     1681 agaactacat cgcctccgcg atccccgcct tcctggccga cacgcgccgg atccagctga
     1741 tcgaggacgg cgacatcgtg gtggtcacac ccgagggggt gagcttcagc gacgccgacg
     1801 gcgcgccggt cgagcacgac gtcgaggagg tcgactggga cgaggagacg gccgagaagt
     1861 cgggctacga gacgttcatg ctcaaggaga tccacgagca gccgcgggcg gtggccgaga
     1921 ccgtggtcga ccggctggcc cacgggcgcg ccgagctggg cgacatcggc atctccgacg
     1981 acgagctgcg cggcctgcgc cgagtggtca tcgtggcctg cggcacctcg taccacgccg
     2041 gcctgatcgg ccgctacgcc atcgagacct ggtcgcgcgt gccggtggag atggacgtgg
     2101 cctcggagtt ccgctaccgg gaccccgtga tcgacgaccg cgacctggtc atcggcatca
     2161 cacagtccgg cgagacgctg gacaccctgg ccgcgatgcg cctggcgcgc gagaagggcg
     2221 ccaaggtcat ggccgtcacc aacatcatgg gcagccaggc cacccgcgac gccgacggcg
     2281 tgctgtacac ccgcgcgggc ctggagatcg gcgtcgccgc caccaagacc ttcgtctccc
     2341 aggtggccgc catgtacctg ttcgcgctga agctggccca ggtgcacgac atgctcgaga
     2401 ccgagcgcca ccgcgagctg gtgcgcgacc tgcgcgacct gtcgcacaag gtcgaggagc
     2461 tgctggccac cgtggacgag ccggtcaagc gcatggccga gcactggaag gagtccggct
     2521 tcttcctgta cctgggccgc cacgcgggcc tggccgtggc gctggagggc gcgctgaagc
     2581 tcaaggagat ctcgtacgtg cccaccgacg cctacgcggc gggtgagatg aagcacggcc
     2641 cgatcgcgct gctggacgag cgcacgccgg tggtggtctc ggccaccgag tcgccggtgc
     2701 tggacaaggt gctgtcgaac atctccgagg tcaccgtgcg cggcgcgcac gtgctggccg
     2761 tggccaccga gggctcgacc gaggtggccg agcacgccga ggaggtggtc tacgtgccca
     2821 agaccaactg gctgctccag cccctgctgg ccgtgatccc gctgcagctg ctggcctact
     2881 acatcgccca gcacaagggc ctgaacgtgg accagccgcg caacctcgcg aagaccgtca
     2941 cggtcgagta gcggtgatag ggctcgatct actggagatc gagcggttcg agcaggtgct
     3001 cgaacgccgc ccccgcatcg ccgagcgcct gttcaccgac gccgagcgcg agtacgccgc
     3061 cggcaaggcg cgccccggcc agcacctggc cgcccgcttc tgcgccaagg aggcggtggc
     3121 caaggcgctg gcgctggagg ggtggagctt caccgacgtc gaggtcgtgg ccaccggcgg
     3181 cgcgcccggc gtccgcctca gcggcagcgc cggcgcccgg gccgaccagc tgggcgtgga
     3241 ggtccgcctg tcgctgaccc acaccgagac caccgccggc gcggtggccc tggccctgtg
     3301 accgcgctgc cggactggct ggatccgctg ttcgaggcgc aggagatgcg cgacgtggac
     3361 gcctgggcga tcgacaccca gggcgtgccg tcgcttgacc tgatggagcg cgcgggggag
     3421 gggctggcgc gcatcacggc ggccgccgcc cgccgcgggc cgatccggat cgtggtcggc
     3481 aagggcaaca acggcggtga cgggctggtg gtcgcgcggc tgctgcgcga ggagggccgc
     3541 gaggtcgacg tgctggccgt ggccgatctc ggcgggctcg agggcgacgc gcgggcgaac
     3601 ctggaccggc tcccgggcga cccgccggag gacttcagcg ccgagggcct ggacggctcg
     3661 ggcgcggtgg tcgacgccat gctgggcacg ggcttcagcg gggagccgcg cgagccgatc
     3721 gcctcggcca tcgccgccat caacgcccaa gacgcgcccg tggtggcctg cgacgtgccc
     3781 tcgggcgtcg gcgcgagcac cggccgcgtg gagggacagg cggtccgggc cacggtgacg
     3841 gggaccttcc acggcccgaa ggtgggcctg tacgtggcgc c
//
LOCUS       DASUPW010000372         2940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1471259, whole genome shotgun sequence.
ACCESSION   DASUPW010000372 DASUPW010000000
VERSION     DASUPW010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471259"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /gene="tsaD"
                     /locus_tag="VFY44_08360"
     CDS             <1..845
                     /gene="tsaD"
                     /locus_tag="VFY44_08360"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX5910493.1"
                     /translation="ELIDAVTADALEQADVTLDRIERVAVTRGPGLIGALLVGVASAK
                     ALAAARGLSLTAVDHLHGHVVASTLGDDPIEAPYLCLVASGGHTFLARVDHPGSYEVI
                     GQTLDDAAGEAFDKGARLLGLGYPGGPELDRLAQQGDPAAFDFPRSAPGDGLDFSFSG
                     VKTALLYAVRDMGEEQAQARRADLAASYQRAIVTALVARTAQAVGREGVARLAIGGGV
                     AANSELRATLAERAAELGVRVWIPARELCTDNAAMIAAAARFTEPIAYPGYLDLDAAA
                     RLAA"
     gene            842..1084
                     /locus_tag="VFY44_08365"
     CDS             842..1084
                     /locus_tag="VFY44_08365"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX5910494.1"
                     /translation="MKLTFYGKAGCHLCEEAYAEVLAVQDEAGFELEVLDVSLDPRMN
                     REYGQRVPVLAADGQDVLELGFDAASVRAVLGRVGP"
     gene            1081..1752
                     /locus_tag="VFY44_08370"
     CDS             1081..1752
                     /locus_tag="VFY44_08370"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEX5910495.1"
                     /translation="MSFREPMTTTPAATPLAADRLSIGVAARLSRYLQVLTQAKKMGK
                     DTISSQELADYTHVNSTQIRRDLSGFGKFGKRGVGYNVDSLVSQIRKILRTSGQHNIA
                     LFGAGHLGQAIAGSSIFADHGFRVVALFDTDPEKIGTAVGENLTVRSAAELAQVVQQE
                     EIVVGVIAVPTAAAQQVADELVQAGVKIIFNYSEALLQVPPEVTVHASSPAVDLLYAL
                     YFYLA"
     gene            1764..2918
                     /locus_tag="VFY44_08375"
     CDS             1764..2918
                     /locus_tag="VFY44_08375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HEX5910496.1"
                     /translation="MTRAREVFEASEDFTVGIEEEFGILDPETRDLVPRFVELRDAAQ
                     ADAVLAESVAGELIESEIEITSGRGVDLADAVARQREHRARLFALAARQGTVLSATAT
                     HPWSPWQDQTVIDTDHYHRVEDGLKYVAWRNNTFSLHVHVGVRGADRAIAVCDRLRPI
                     LPELLALSANSVFLDGRDSGLQSARSQIFTKSFPRCGIPEPFGGWQAWADYVDLLVQT
                     NSIVEHTQLWWSVRPHHSFGTVEVRICDAQSGAEESTALAGLIVACVAQAAADHDEGV
                     AFADPPGRLVEENFWRAIRYGLEGRMIDTERLEEFPAEAIGDRLMAWTAPARAQLRLD
                     VGVPEVNGAARQRAALADGATMREVFAAEVAHSGRTYAGGEVVPEPATRR"
BASE COUNT          409 a         1048 c         1077 g          406 t
ORIGIN      
        1 tggagctgat cgacgcggtc acggccgacg cgctcgagca ggcggacgtg acgctggacc
       61 ggatcgagcg cgtggccgtg acgcgcggcc cggggctgat cggggcgctg ctggtcggcg
      121 tggcgtcggc caaggcgctg gccgccgccc gcgggttgtc cctgaccgcg gtcgaccacc
      181 tgcacggcca cgtggtggcc agcaccctcg gcgacgaccc gatcgaggcg ccctacctgt
      241 gcctggtggc cagcggcggc cacacctttc tggcccgggt cgaccacccg ggctcctacg
      301 aggtgatcgg ccagacgctg gacgacgcgg cgggcgaggc cttcgacaag ggcgcccggc
      361 tgctgggcct gggctacccg ggcggcccgg agctggaccg cctggcccag cagggcgatc
      421 cggccgcgtt cgacttcccg cgctcggcgc cgggcgacgg cctggacttc tcgttcagcg
      481 gggtcaagac cgcgctgctg tacgccgtgc gcgacatggg ggaggagcag gcgcaggcgc
      541 gccgggccga cctggccgcg tcctaccagc gggcgatcgt gacggcgctc gtggcgcgca
      601 ccgcccaggc cgtgggccgc gagggcgtgg cccgcctggc catcgggggc ggcgtggccg
      661 ccaacagcga gctgcgcgcc acgctggccg agcgcgccgc cgagctgggc gtgcgcgtgt
      721 ggatcccggc gcgcgagctg tgcaccgaca acgcggccat gatcgccgcc gcggcccggt
      781 tcaccgagcc gatcgcctac cccggctatc tggacctgga cgcggcggcc aggctggccg
      841 cgtgaagctc accttctacg gaaaggcggg ctgtcacctg tgcgaggaag cgtacgccga
      901 ggtcctggcc gtccaggacg aggcgggctt cgagctcgag gtactcgacg tttccctgga
      961 tccgcgcatg aaccgcgagt acggccagcg ggtgccggtg ctggccgcag acggccagga
     1021 tgtgctcgag ctgggcttcg acgccgcttc cgtacgcgcc gtgctgggta gagtggggcc
     1081 gtgagcttcc gcgagcccat gacgacaacc cctgccgcga cgcccctggc ggccgaccgg
     1141 ctgtccatcg gcgtggccgc ccggctgtcg cgctacctgc aggtgctgac ccaggccaag
     1201 aagatgggca aggacaccat ctcctcccag gagctggccg actacacgca cgtgaactcc
     1261 acccagatcc ggcgcgacct gtccgggttc ggcaagttcg gcaagcgcgg ggtgggctac
     1321 aacgtggact cgctggtctc gcagatccgc aagatcctgc gcaccagcgg ccagcacaac
     1381 atcgccctgt tcggtgccgg ccacctgggc caggccatcg ccggctccag catcttcgcc
     1441 gaccacggct tccgggtcgt ggccctgttc gacaccgacc cggagaagat cggcacggcg
     1501 gtgggcgaga acctgaccgt ccgctcggct gccgagctgg cgcaggtggt gcagcaggag
     1561 gagatcgtcg tgggcgtgat cgcggtgccc accgcggcgg cccagcaggt ggccgacgag
     1621 ctggtgcagg ccggggtcaa gatcatcttc aactactccg aggccctgct gcaggtgccg
     1681 cccgaggtca ccgtgcacgc ctcctcgccc gccgtggacc tgctgtacgc gctctacttc
     1741 tacctggcct gagcatgctg gacatgacgc gagcccggga ggtcttcgag gcctccgagg
     1801 acttcaccgt cggcatcgag gaggagttcg gcatcctcga tcccgagacg cgggacctgg
     1861 tgccgcggtt cgtggagctg cgcgacgccg cccaggccga tgcggtgctg gccgagtcgg
     1921 tggccggcga gctgatcgag tccgagatcg agatcacgtc cggccgcggc gtggacctgg
     1981 ccgacgcggt ggcccgccag cgtgagcacc gggcgcggct gttcgcgctc gcggcgcgcc
     2041 agggcacggt gctgtcggcc accgcgacgc acccctggag cccgtggcag gaccagacgg
     2101 tgatcgacac cgaccactac caccgcgtcg aggacgggct gaagtacgtg gcctggcgca
     2161 acaacacctt cagcctgcac gtccacgtcg gcgtccgcgg cgccgaccgc gcgatcgccg
     2221 tgtgcgaccg cctccggccg atcctgcccg agctgctggc gctgtcggcc aactcggtgt
     2281 tcctggacgg ccgtgactcg ggcctgcagt cggcgcggtc gcagatcttc accaagagct
     2341 ttccccgctg cgggatcccg gagccgttcg gcggctggca ggcatgggcc gactacgtcg
     2401 acctcctggt gcagaccaac tcgatcgtcg agcacaccca gctgtggtgg agcgtgcgcc
     2461 cgcaccactc cttcggcacc gtcgaggtcc gcatctgcga cgcccagtcc ggagccgagg
     2521 agtccaccgc cctggccggc ctgatcgtgg cctgcgtggc ccaggccgcc gccgaccacg
     2581 acgagggcgt ggccttcgcc gatcctcccg ggcgcctggt cgaggagaac ttctggcgcg
     2641 ccatccggta cgggctcgag gggcggatga tcgacaccga gcgcctcgag gagttcccgg
     2701 ccgaggccat cggcgaccgc ctgatggcct ggacggcccc cgccagggcg cagctcaggc
     2761 tggacgtggg ggtgccggag gtcaacggcg ccgcccggca acgggccgcg ctggccgatg
     2821 gtgcaaccat gcgcgaggtc ttcgccgccg aggtggccca ttccgggcgc acctacgcgg
     2881 gaggcgaggt cgtgcccgag ccagcaaccc ggaggtagtg atgagcgcag aaggacgaga
//
LOCUS       DASUPW010000373         3674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1749046, whole genome shotgun sequence.
ACCESSION   DASUPW010000373 DASUPW010000000
VERSION     DASUPW010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1749046"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VFY44_08380"
     CDS             <1..515
                     /locus_tag="VFY44_08380"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX5910497.1"
                     /translation="IAVAEFARHVAGMDGANSTEFDPETPYPVVDLLPEQKEVRDMGG
                     TMRLGADPVKLHEGTRAREIYDEPVIYERHRHRYEVNNHLRRKLEALGLVCSGTSPDD
                     RLVEAIELPDHPFFVASQYHPEFKSRPLRPQPLFRDFVGAALDRVRSREPQGEVSASR
                     GDAETADSNA"
     gene            530..727
                     /locus_tag="VFY44_08385"
     CDS             530..727
                     /locus_tag="VFY44_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910498.1"
                     /translation="MADLDKRGSYVPRRTREKRAYQALVGGGGLAVAAVVLFVIGMGG
                     LGFVALILAAIAGFVFFRSVS"
     gene            727..1857
                     /locus_tag="VFY44_08390"
     CDS             727..1857
                     /locus_tag="VFY44_08390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5910499.1"
                     /translation="MAPAATEAERERLVADFTHYCEIESPSTRERPMADAVAADLADL
                     GLEVHEDDSAAATGSDSGNLTARVPGPEGARTVLLCAHLDTVPLAAPVEVEREGGVLR
                     NRNEGILGADNKAAVATIMGAVRHLIERGGPPVGLELVFTTMEEPGLIGAKEVDRHSL
                     RSEFGFVFDHASPIGELIVAAPTYYKLLARFTGVAAHAGMEPELGRSAIEAASRAIAA
                     MRLGRLDEQTTANAGVIRGGTAKNVVAERCEIEMEARSLDDAVAGDLVGEMVDRCSDA
                     AGEVECDVDTTVEQLSRAFKLARSAPPVQAACAALEDLGIEPVPRSTGGGSDANVLIA
                     AGLPVLNVANGTERAHQSDESVTVEALERMLDLTVRLVERSA"
     gene            1857..2387
                     /locus_tag="VFY44_08395"
     CDS             1857..2387
                     /locus_tag="VFY44_08395"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5910500.1"
                     /translation="MDFERIDSEDVWQGHVGGVRVDTFRYADGGEAKREVFTHPGAVS
                     ILPFDGENIWMVRQPREPVGEPELLELPAGKIDKPGEELEELALRELQEEIGKGARSV
                     RHLCTFYNSPGLLTEEHHLYLATDLYDSRRDSGEDERIEIETLAVADLDDVIPAIKDA
                     KTLIGLLWFRAYLQDG"
     gene            2428..3366
                     /gene="xerD"
                     /locus_tag="VFY44_08400"
     CDS             2428..3366
                     /gene="xerD"
                     /locus_tag="VFY44_08400"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX5910501.1"
                     /translation="MATAAAPIAQRPFEHLVLDFLAYLEFERGLSRNTLEAYRSDLLQ
                     FGRFLEARGLAVVAVTPPQLEDFLAELAGTAGGSASPATIHRKAACLRSFYRHLRRDG
                     LLATDPTASLAAPRRGRKLPQVLTRGQVDKLLAQPKGTAPTDLRDRALLELMYACGLR
                     ASEAIGLQVSDVDLDEGFLRARGKGSKERVVPVGQTAVGAVRRYLERGRPALVGDSAE
                     PHLFVNFRGGGLTRQGLYKIVRRHAVSAGLADRMSPHTLRHTFATHLLAGGCDLRAVQ
                     EMLGHADVATTQLYTHLSSERLKDVYFRAHPRAAAR"
     gene            3379..>3674
                     /locus_tag="VFY44_08405"
     CDS             3379..>3674
                     /locus_tag="VFY44_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910502.1"
                     /translation="MRSRPILLAVLAAGVLPAAGCGDQRPPVATGPVAPSAKTVEYEF
                     PRAGMTLRLPANLQVERLARLPAVFRAGLVEPFVSAFAYRRAEQVPRNARELAAA"
BASE COUNT          499 a         1353 c         1352 g          470 t
ORIGIN      
        1 agatcgcggt ggccgagttc gcgcgccacg tggccggcat ggacggggcc aactcgaccg
       61 agttcgaccc cgagacgccg tacccggtgg tcgacctcct gcccgagcag aaggaggtcc
      121 gcgacatggg cggcaccatg cgcctgggcg ccgacccggt gaagctccac gagggcacgc
      181 gcgcccgcga gatctacgac gagcccgtga tctacgagcg ccaccgtcac cgctacgagg
      241 tcaacaacca cctgcgccgc aagctcgagg cgctgggcct ggtctgctcg ggcacgtcgc
      301 ccgacgaccg gctggtcgag gccatcgagc tgccggatca ccccttcttc gtggcctcgc
      361 agtatcaccc cgagttcaag tcgcgcccgc tgcgcccgca gccgctgttc cgcgacttcg
      421 tgggcgccgc gctcgaccgg gtccgcagcc gcgagcccca gggcgaggtc agcgccagcc
      481 gcggcgacgc cgagacggct gacagcaacg cctagatacc gttccgcgca tggctgatct
      541 cgacaagcgc ggttcctacg tcccccggcg cacccgcgag aagcgggcct accaggctct
      601 ggtggggggt ggcggcctgg cggtcgcggc cgtggtgctg ttcgtgatcg gcatgggcgg
      661 cctcggcttc gtggcgctga tcctggccgc catcgccggc ttcgtgttct tccgctccgt
      721 cagctagtgg cgcccgcggc caccgaggcc gagcgggagc ggctggtcgc cgacttcacg
      781 cactactgcg agatcgagag cccgtccacg cgcgagcggc cgatggccga cgcggtggcc
      841 gccgacctgg ccgacctggg gctcgaggtc cacgaggacg acagcgcggc ggccacgggc
      901 tccgacagcg ggaacctgac cgcgcgcgtg cccgggcccg agggcgcgcg caccgtgctc
      961 ctgtgcgccc acctggacac ggtcccgctg gcggcgccgg tcgaggtgga gcgcgagggg
     1021 ggcgtgctgc gcaaccgcaa cgagggcatc ctgggcgccg acaacaaggc cgcggtggcc
     1081 acgatcatgg gcgcggtccg ccacctgatc gagcgcggcg gcccgccggt gggcctggag
     1141 ctggtgttca ccaccatgga ggagcccggg ctgatcggcg ccaaggaggt cgaccgccac
     1201 tccctgcgca gcgagttcgg gttcgtgttc gaccacgcga gcccgatcgg cgagctgatc
     1261 gtggccgcgc ccacctacta caagctgctg gcccgcttca ccggggtcgc ggcccacgcc
     1321 gggatggagc ccgagctggg ccgcagcgcc atcgaggcgg cctcgcgcgc catcgcggcg
     1381 atgcgcctgg ggcgactgga cgagcagacc accgccaacg ccggcgtgat ccgcggcggc
     1441 acggccaaga acgtcgtggc cgagcggtgc gagatcgaga tggaggcccg cagcctggac
     1501 gacgccgtcg ccggcgacct ggtgggggag atggtcgacc gctgctccga cgccgcgggc
     1561 gaggtcgagt gcgacgtgga caccaccgtc gagcagctct cgcgcgcgtt caagctcgcg
     1621 cgcagcgcgc cgccggtcca ggccgcgtgc gcggcgctcg aggacctggg catcgagccg
     1681 gtcccgcggt ccacgggcgg cggcagcgac gccaacgtgc tcatcgccgc cgggctgccc
     1741 gtgctgaacg tggccaacgg caccgagcgt gcccaccagt ccgacgagag cgtgaccgtc
     1801 gaggccctgg agcggatgct cgacctgacg gtgcgcctgg tggagcgcag cgcctgatgg
     1861 acttcgagcg catcgacagc gaggacgtct ggcagggcca cgtcggcggt gtgcgcgtgg
     1921 acaccttccg gtacgccgac gggggcgagg ccaagcggga ggtgttcacc catcccggcg
     1981 ccgtcagcat cctgccgttc gacggcgaga acatctggat ggtccgccag ccgcgcgagc
     2041 cggtcggcga gcccgagctg ctggagctgc ccgcgggcaa gatcgacaag ccgggggagg
     2101 agctcgagga gctggcgctg cgcgagctgc aggaggagat cggcaagggg gcgcgctcgg
     2161 tgcggcacct gtgcaccttc tacaactctc ccggcctgct gaccgaggaa caccacctgt
     2221 acctggccac cgacctgtac gactcccgcc gcgactcggg cgaggacgag cggatcgaga
     2281 tcgagacgct ggcggtggcc gacctggacg acgtcatccc ggcgatcaag gacgcgaaga
     2341 ccctgatcgg cctgctgtgg ttccgggcct acctgcaaga cggctagcag ggcgaggaca
     2401 ccgccggggg cgggcgaatc ccggagcgtg gccaccgcgg cagcgcccat agcccagcgc
     2461 ccgttcgagc acctggtgct ggacttcctg gcgtacctgg agttcgagcg tgggctctcg
     2521 cgcaacaccc tggaggccta ccgctcggac ctgctccagt tcgggcgctt cctggaggcg
     2581 cgcgggctgg ccgtcgtcgc ggtcacgccg ccgcagctgg aggacttcct ggccgagctg
     2641 gccgggaccg cgggcggctc ggcctcgccg gccacgatcc accgcaaggc cgcgtgcctg
     2701 cgttccttct accgccacct gcgccgcgac gggctgctgg ccaccgatcc cacggccagc
     2761 ctggctgcgc cccgccgggg ccgcaagctg ccccaggtgc tcacccgcgg ccaggtcgac
     2821 aagctgctgg cccagccgaa ggggaccgcg cccaccgacc tgcgcgaccg ggcgctgctg
     2881 gagctgatgt acgcctgcgg cctgcgcgcg tccgaggcca tcgggctcca ggtctccgac
     2941 gtggacctcg acgaaggctt cctgcgcgcc cggggcaagg gctcgaagga gcgcgtcgtg
     3001 cccgtgggcc agaccgccgt cggcgcggtg cgccgctacc tggagcgcgg gcggccggcg
     3061 ctggtgggcg actcggccga gccgcacctg ttcgtgaact tccgcggcgg cggcctgacc
     3121 cgccaggggc tgtacaagat cgtccgccgc cacgccgtct ccgccggcct ggccgaccgg
     3181 atgagcccgc acacgctgcg ccacacgttc gccacccacc tgctggcggg cggctgtgac
     3241 ctgcgcgccg tccaggagat gctcggccac gccgacgtgg ccacgacgca gctgtacacg
     3301 cacctctcga gcgagcggct caaggacgtg tacttccgcg cgcacccgcg cgcggccgct
     3361 cgctgataca acggcggcgt gcgctcccgc ccgatccttc tcgcggtgct ggcggccggg
     3421 gtgctcccgg cggccgggtg cggcgaccag cgcccgcccg tggccaccgg cccggtggcg
     3481 ccgagcgcca agacggtcga gtacgagttc ccgcgcgcgg gcatgaccct gcgcctgccg
     3541 gccaacctgc aggtcgagcg gctggcgcgg ctcccggcgg tgttccgggc cggcctggtg
     3601 gagccgttcg tgtccgcgtt cgcctaccgg cgggccgagc aggtgccgcg caacgcccgc
     3661 gagctggccg ccgc
//
LOCUS       DASUPW010000374         2801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1610047, whole genome shotgun sequence.
ACCESSION   DASUPW010000374 DASUPW010000000
VERSION     DASUPW010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610047"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VFY44_08410"
     CDS             complement(<1..798)
                     /locus_tag="VFY44_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006681752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX5910503.1"
                     /translation="MREQIELSNEVAAELAGANDMIMRTLEERLDCDVFLRGNILTLD
                     GQPDDVAAGREVVRELSDLVGQGHDVAPGTIEAIGSALDRHESPSQILEDVVWRHRGL
                     KVAPKTVTQKRYVDAIRESTITVGIGPAGTGKSFLAVAMAVAALQRREVNRIVLTRPA
                     VEAGERLGFLPGDLMAKVDPYLRPLFDALYDMLEPEKVNGHLERGVIEVAPLAFMRGR
                     TLNDAFIIVDEAQNTTPEQMKMVLTRLGFNSKMVVTGDVTQVDLPRDQ"
     gene            complement(798..1229)
                     /locus_tag="VFY44_08415"
     CDS             complement(798..1229)
                     /locus_tag="VFY44_08415"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX5910504.1"
                     /translation="MAVLDQLQSDANAALKAGDRERAGALRVVLSQLQLAVKEGSDDE
                     IAVLQRERKRRLESAEAFREGGRDELAGEAERDAELISTYLPEQISDEELRALVADAV
                     AASGATSPKDMGKVMSMVMPQVQGRADGKRVSGAAKELLTP"
     gene            1250..1936
                     /locus_tag="VFY44_08420"
     CDS             1250..1936
                     /locus_tag="VFY44_08420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEX5910505.1"
                     /translation="MPAWKAATFDCYGTLIDWEGGAAAFLYDVARRNGDDDPGPGYLL
                     RRRWEEIQFEHIQQDYRSYNVVLEESLREWVGERGYRWNVNVGYDFARSMQSWQPFPD
                     TIPALTRLREAGVPLAIVSNSESAIMDHTLRQLAPLEFDHVVLADEVRCYKPDRRPFT
                     RMLDKLSAAPEDVLHVAFGFKYDIGRAKDLGMQTAWINRHREPLPDQDTVPDHEWRDL
                     WGLAELAERD"
     gene            1946..>2801
                     /locus_tag="VFY44_08425"
     CDS             1946..>2801
                     /locus_tag="VFY44_08425"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX5910506.1"
                     /translation="MDYGELLLLAGALLATGIAASLVAGSLRVPGLIVVLGLAMAIGS
                     DGLGLIHFGGSEADVELARTIGVIALVFILFEGGLASGWGEIRPVLRTSVMLAIFATV
                     AQAVIVGLAAAWVLDLSTEEGLLLGSIVSATDSAAIFSVLRGSSLKKRLARILEGESG
                     MNDPVAIVLVLGFIEFIQDPDYTLLDMAYLAVVELGLGVLIGLVVGRLAVLTFQSARI
                     TAPGLFPVASLAFAALGFGIADVVHASGFIAVYLVGLALGSGSIPGKRTIDEFHDGLA
                     WISQIAMFM"
BASE COUNT          377 a          988 c          973 g          463 t
ORIGIN      
        1 ctggtcgcgc ggcaggtcga cctgggtcac gtcgccggtg acgaccatct tcgagttgaa
       61 gcccagccgc gtgagcacca tcttcatctg ctccggcgtg gtgttctggg cctcgtcgac
      121 gatgatgaac gcgtcgttga gggtgcgccc gcgcatgaag gccagcggcg cgacctcgat
      181 cacgccgcgc tccaggtgcc cgttgacctt ctcgggctcg agcatgtcgt agagcgcgtc
      241 gaacagcggc cgcaggtagg ggtcgacctt cgccatcagg tcgccgggca ggaagcccag
      301 gcgctcgccg gcctcgacgg cggggcgcgt cagcacgatc cggttgacct cgcgccgctg
      361 cagggcggcc acggccatgg ccaccgccag gaacgacttg ccggtgcccg ccgggccgat
      421 gccgacggtg atggtggact cgcggatggc gtccacgtag cgcttctggg tcaccgtctt
      481 gggcgccacc ttcagcccgc ggtggcgcca gaccacgtcc tccaggatct ggctggggct
      541 ctcgtggcgg tccagcgccg agccgatggc ctcgatcgtc cccggcgcca cgtcgtggcc
      601 ctgcccgacc aggtcggaca gctcgcggac cacctcgcgg ccggcggcca cgtcgtcggg
      661 ctggccgtcc agcgtcagga tgttgccgcg caggaacacg tcgcagtcca gccgctcctc
      721 cagcgtgcgc atgatcatgt cgttggcgcc cgccagctcg gcggcgacct cgttgctgag
      781 ctcgatctgc tctcgcacta cggagtcagg agctccttcg cggcgccgct gacgcgcttg
      841 ccgtcggcgc ggccctggac ctggggcatg accatcgaca tgaccttgcc catgtccttc
      901 ggtgacgtcg cccccgacgc ggcgaccgcg tcggccacca gcgccctcag ctcctcgtcg
      961 gagatctgct cggggaggta ggtcgagatg agctcagcgt cacgctctgc ctcgccggcc
     1021 agctcgtcgc gcccgccctc gcggaacgcc tcggcggact ccagccggcg cttgcgctcg
     1081 cgctggagca cggcgatctc gtcgtcgctg ccctccttga ccgcgagctg gagctgggag
     1141 agcaccacgc gcagcgcgcc cgcacgctcg cggtcgcccg ccttgagcgc ggcgttcgcg
     1201 tcggactgca gttggtcgag cacggccatt agggtcaagt gtaggccgca tgccagcgtg
     1261 gaaagctgca accttcgact gttacgggac cctcatcgac tgggagggag gcgccgcagc
     1321 gttcctgtac gacgtcgccc gccgcaacgg cgacgacgat cccgggcccg ggtacctgct
     1381 gcgccggcgc tgggaggaga tccagttcga gcacatccag caggactacc gcagctacaa
     1441 cgtcgtgctc gaggagagcc tgcgcgagtg ggtcggcgag cgcggctacc gctggaacgt
     1501 gaacgtgggc tacgacttcg cgcgctcgat gcagtcctgg cagccgttcc ccgacaccat
     1561 ccccgcgctg accagactgc gtgaggccgg cgtcccgctg gccatcgtgt cgaattccga
     1621 gagcgcgatc atggaccaca cgctgcgcca gctggcaccg ctggagttcg atcatgtggt
     1681 cttggccgac gaggtgcgct gttacaagcc cgaccgccgt ccgttcacgc ggatgctgga
     1741 caagctgagc gccgccccgg aggacgtcct gcacgtcgcc ttcggcttca agtacgacat
     1801 cggcagggcc aaggacctgg gcatgcagac cgcgtggatc aaccgccacc gcgagccgct
     1861 gcccgaccag gacacggtgc ccgaccacga gtggcgcgac ctctggggtc tcgccgagct
     1921 ggccgaacgc gactagagcc cggcgatgga ctacggagaa ctcctcctgc tcgcaggcgc
     1981 gctgctggct accgggatag ccgcatcgct ggtcgccggc agcctgcgcg tgcccggcct
     2041 gatcgtggtc ctgggcctgg ccatggccat cggctcggac gggctcggcc tgatccactt
     2101 cggcggctcg gaggcggacg tcgagctggc ccgcacgatc ggggtcatcg cgctggtgtt
     2161 catcctgttc gagggcggcc tggcctcggg ctggggcgag atccggccgg tcctgcgcac
     2221 cagcgtgatg ctggcgatct tcgccaccgt cgcccaggcg gtcatcgtgg gcctggcggc
     2281 cgcatgggtg ctggacctga gcaccgagga ggggctcctg ctgggctcga tcgtgtccgc
     2341 caccgacagc gccgcgatct tctccgtgct ccgcgggtcg tcgctcaaga agcggctggc
     2401 ccggatcctg gagggcgagt cgggcatgaa cgacccggtg gccatcgtcc tggtgctggg
     2461 cttcatcgag ttcatccagg accccgacta cacgctgctg gacatggcct acctggccgt
     2521 ggtcgagctc gggctgggtg tgctcatcgg cctggtcgtc ggccgcctgg cggtgctgac
     2581 cttccagagc gcccgcatca cggcgcccgg cctgttcccc gtcgcgtcgc tggccttcgc
     2641 cgcgctgggc ttcggcatcg ccgacgtggt gcacgcgtcg ggcttcatcg ccgtctacct
     2701 ggtgggcctg gcgctgggga gcgggtcgat cccgggcaag cgcacgatcg acgagttcca
     2761 cgacggcctg gcctggatca gccagatcgc gatgttcatg a
//
LOCUS       DASUPW010000375         3726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1195729, whole genome shotgun sequence.
ACCESSION   DASUPW010000375 DASUPW010000000
VERSION     DASUPW010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195729"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="VFY44_08430"
     CDS             complement(<1..979)
                     /locus_tag="VFY44_08430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5910507.1"
                     /translation="MRADGLRLRLAPPDGPRPGRDGRLSRVLITAFGDPGHAFPAIAL
                     GRELAARGHEVCLETWDRWQEDVEREGMEFAPAPEYDVWPGKGLTPYQAAVRAAHETV
                     ALIDRFDPEVVVADILTVAGGLAAQMARRPWVTLVPHVYPVPEAGMAPYSSGARMPRT
                     RVGRIWWSGFERALMRGAERGRDELNGARERVGLPPLDHTHGGISPTLALVATFPQLE
                     YPRLDVPPAIRVTGPLLWERPAEPVELPPGDDPLVLIAPSTSQDPDQRMVRASLAGLA
                     GQPVRVLATLNHRPSPVPLDVPDNARVVDWLSYARTMPRCAAVVCHSGHG"
     gene            complement(907..1950)
                     /locus_tag="VFY44_08435"
     CDS             complement(907..1950)
                     /locus_tag="VFY44_08435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX5910508.1"
                     /translation="MRLGLNVGYWGLGLTAAEQLEIVREAEAAGFDSVWAAEAYGSDT
                     ATVLTWLGAQTEKLKLGSAIFQMPGRSPAMTAMTAATIDNISGGRMLLGIGSSGPQVA
                     EGWHGQPFARQLQRTREYVGIVRKALARERLEWQGEEYTLPLPNGPGKALKLMISPVQ
                     DRIPIYIAAIGPKNTQLTGEIADGWLPTFFSPEHVSDSRTLLQEGADRAGNGKQIDDS
                     FDIAPVVNVSIDDDLDRARDSMRPVLALYVGGMGSRKKNFYNAIVQRYGFEDAAKEVQ
                     DLYLDGKKDEAAAALPVELIDMVSLCGPKDRVAERLEVYRDAGVGTLICAPMAFDFDS
                     RRQMVHDLAEMAA"
     gene            2078..2530
                     /locus_tag="VFY44_08440"
     CDS             2078..2530
                     /locus_tag="VFY44_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910509.1"
                     /translation="MPAHTRQAMLEGIGKTTIIVGGYSDGQGGICPMLAAHRNGGRTS
                     FATFAHAWDRYTKADGVARTATERELQTLRSMLEASIEAEEIVGQGVLGEAVKQHRHS
                     QEDRARREEFDVEPVEPLSEGQILSTRRRPARTAPPARDTPVMSTART"
     gene            complement(2454..3176)
                     /locus_tag="VFY44_08445"
     CDS             complement(2454..3176)
                     /locus_tag="VFY44_08445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5910510.1"
                     /translation="MSERAALVTGGSSGIGLAIARMLGSEGYGITLSARRPEKLEGAA
                     AGLREEGFDVEAVPANMADESELQALLERHKERFGRLDVLVNNAGVGIGGPFAEAETK
                     KVDMQIAVNLRAVYLLTRDAIPLLKEAGAEHGKALIVNTASIAGKHGQGWLSAYSATK
                     AGVVGLSQALYKEHAGDGIQTTALCPGFVATAMTDWIEEQVPKDQMIQPEDVAGAVRY
                     VLSTSPACHVPEVQFVRAGDVY"
     gene            3250..>3726
                     /locus_tag="VFY44_08450"
     CDS             3250..>3726
                     /locus_tag="VFY44_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910511.1"
                     /translation="MRRPLYALVLALVVGIAGCGGDDSSDSADSPPAPAKAADFPKAD
                     GKTVAQLRKEASGAGPVLAPTQSQLTPGKIRYGFALFDRDRTQIADVPVALYVAPAGG
                     EAKGPVPAKWESLKVQAQFQSRSTADDPDAAKAIYAAQLDFPKPGRYEVLAIARLDG"
BASE COUNT          510 a         1372 c         1330 g          514 t
ORIGIN      
        1 tcccgtggcc ggaatggcac acgacggccg cgcaccgcgg catcgtgcgc gcgtacgaga
       61 gccagtcgac cacgcgggcg ttgtccggca cgtccagcgg cacgggggag gggcggtggt
      121 tcagcgtggc caggacgcgg acgggctggc cggccaggcc ggccagcgac gcacggacca
      181 tgcgctggtc ggggtcctgg gaggtgctgg gagcgatgag caccagcggg tcgtcgccgg
      241 gcggcagctc gaccggctcc gccgggcgct cccacagcag cgggcccgtc acgcggatcg
      301 ccggtggcac gtccaggcgc gggtactcga gctgggggaa ggtggccacc agcgccaggg
      361 tgggggagat gccgccgtgg gtgtggtcca gcgggggcag ccccacccgc tcgcgggcgc
      421 cgttgagctc gtcgcggccg cgctcggcgc cgcgcatcag cgcgcgctcg aagccgctcc
      481 accagatccg gcccacgcgc gtgcggggca tgcgggcacc ggacgagtac ggggccatgc
      541 cggcctcggg cacggggtag acgtgcggga ccagcgtcac ccacggtcgc cgggccatct
      601 gcgcggccag cccgccggcc acggtcagga tgtcggccac caccacctcg gggtcgaagc
      661 ggtcgatcag cgcgaccgtc tcgtgtgccg cgcgcacggc ggcctggtag ggcgtcagcc
      721 ccttgcccgg ccagacgtcg tactccggcg ccggggcgaa ctccatgccc tcgcgctcga
      781 cgtcctcctg ccagcggtcc caggtctcca ggcacacctc gtgcccgcgg gccgccagct
      841 cgcggcccag ggcgatcgcg gggaacgcgt gcccggggtc gccgaaggcg gtgatcagta
      901 cgcggctcag gcggccatct cggccaggtc gtggaccatc tggcggcgcg agtcgaagtc
      961 gaaggccatc ggcgcgcaga tcagcgtgcc cacgccggcg tcgcggtaga cctcgagccg
     1021 ctcggccacg cggtccttgg gcccgcacag cgagaccatg tcgatcagct ccacgggcag
     1081 cgccgccgcg gcctcgtcct tcttgccgtc caggtagagg tcctggacct ccttggccgc
     1141 gtcctcgaag ccgtaccgct ggacgatcgc gttgtagaag ttcttcttgc gcgagcccat
     1201 gccgcccacg tacagggcca ggacggggcg catggagtcg cgggcgcggt ccaggtcgtc
     1261 gtcgatcgac acgttgacca ccggcgcgat gtcgaacgag tcgtcgatct gcttgccgtt
     1321 gcccgcgcgg tcggcgccct cctgcagcag cgtgcgcgag tcgctgacgt gctcggggct
     1381 gaagaaggtg ggcagccagc cgtcggcgat ctcgccggtg agctgggtgt tcttgggccc
     1441 gatcgcggcg atgtagatgg ggatccggtc ctggaccggg ctgatcatca gcttcagcgc
     1501 cttgccgggg ccgttgggca ggggcagggt gtactcctcg ccctgccact ccaggcgctc
     1561 gcgggcgagg gccttgcgca cgatgcccac gtactcgcgc gtgcgctgga gctggcgggc
     1621 gaacggctgg ccgtgccagc cctcggccac ctggggcccg gaggagccga tccccagcag
     1681 catccgcccg ccggagatgt tgtcgatggt ggccgcggtc atggcggtca tggccggcga
     1741 gcgcccgggc atctggaaga tggcggagcc cagcttgagc ttctcggtct gcgcgcccag
     1801 ccaggtcagc accgtggcgg tgtccgatcc gtacgcctcg gcggcccaga cggagtcgaa
     1861 gcccgcggcc tcggcctcgc gcacgatctc cagctgctcg gcggcggtca gccccagacc
     1921 ccagtatccg acgttcagac caagacgcat gttgcaactc tcctctcaga agcaggaacg
     1981 gccttgaacc ggccgacgcg cgcccctacg gtgactggcc gtgaggcgac ggcatcgtat
     2041 tcggcccgcg cgtgagctgc ggacagcaat cgactgcatg cccgcgcaca cgcgccaggc
     2101 aatgctcgag ggcatcggca agaccacgat catcgtgggc ggctacagcg acggccaggg
     2161 cggcatctgc ccgatgctgg ccgcgcaccg caacggcggc cggacctcgt tcgccacctt
     2221 cgcccacgcg tgggaccgct acaccaaggc cgacggcgtg gcccgcacgg ccaccgagcg
     2281 cgagctgcag acgctgcggt cgatgctcga ggccagcatc gaggccgagg agatcgtcgg
     2341 ccagggcgtc ctgggcgagg cggtcaagca gcaccgccac tcccaggagg accgggcccg
     2401 gcgcgaggag ttcgacgtcg agccggtgga gcccctctcg gagggccaga tcctcagtac
     2461 acgtcgccgg cccgcacgaa ctgcacctcc ggcacgtgac acgccggtga tgtcgacagc
     2521 acgtacctga ccgctccggc cacgtcctcg ggctggatca tctggtcctt gggcacctgc
     2581 tcctcgatcc agtcggtcat ggccgtggcc acgaagcccg ggcacagcgc ggtggtctgg
     2641 atgccgtcgc ccgcgtgctc cttgtagagc gcctggctca ggccgaccac gccggccttg
     2701 gtggccgagt acgcgctcag ccagccctgg ccgtgcttgc cggcgatcga cgcggtgttc
     2761 acgatcagcg ccttgccgtg ctcggcgccc gcctccttga gcagggggat cgcgtcgcgg
     2821 gtcagcaggt agaccgcgcg caggttcacg gcgatctgca tgtcgacctt cttggtctcg
     2881 gcctcggcga acgggccgcc gatgcccacc ccggcgttgt tgaccagcac gtccaggcgc
     2941 ccgaaccgct ccttgtggcg ctccagcagg gcctgaagct cggactcgtc ggccatgttc
     3001 gcgggcacgg cctcgacgtc gaagccctct tcgcgcagcc cggcggcggc gccctcgagc
     3061 ttctccggcc tgcgcgcgga cagcgtgatc ccgtagccct cggaccccag catccgggcg
     3121 atcgccagcc cgatcccgct cgatccgccc gtcaccagcg cggcacgttc cgacacctgc
     3181 tcctcctccg ttggactggc gccgagacta tctccgcacg ccccgaccgc gttccctagc
     3241 cttcgcgcgg tgcgccgccc cctctacgcc ctcgtgctcg cgctcgtggt cggcatcgcc
     3301 ggctgcgggg gcgacgactc gtccgacagc gccgacagcc cgcccgcgcc cgccaaggcc
     3361 gccgacttcc ccaaggcgga cgggaagacg gtggcccagc tgcgcaagga ggccagcggg
     3421 gcaggccccg tgctggcgcc cacgcagagc cagctgacgc cgggcaagat ccgctacggg
     3481 ttcgcgctct tcgaccgcga ccggacgcag atcgccgacg tgccggtggc cctgtacgtg
     3541 gccccggcgg ggggcgaggc caagggcccc gtgccggcca agtgggagtc gctgaaggtg
     3601 caggcccagt tccagagccg ctcgaccgcc gacgaccccg acgcggccaa ggccatctac
     3661 gccgcgcagc tggacttccc caagcccggc cgctacgagg tcctggccat cgcgcgcctg
     3721 gacggc
//
LOCUS       DASUPW010000376         4855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1057214, whole genome shotgun sequence.
ACCESSION   DASUPW010000376 DASUPW010000000
VERSION     DASUPW010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057214"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VFY44_08455"
     CDS             <1..543
                     /locus_tag="VFY44_08455"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX5910512.1"
                     /translation="TYDDLRDASAERVASTPGLDGIAVGGSLGQEKEQMHAVVERSLR
                     PAPDEWPRHLLGIGDVDVILAAVRAGIDTFDCATPTRLARHGTALVPDPERRWKIDLT
                     KSEWRDDPRPLFEGAATPHSRAYLHYLAKGRELTGARILTLHNLDFMARLMSGIRAAI
                     RDGSWEQYAEKVLAGEEPYG"
     gene            complement(540..1742)
                     /locus_tag="VFY44_08460"
     CDS             complement(540..1742)
                     /locus_tag="VFY44_08460"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5910513.1"
                     /translation="MAAPGEIAGRYALDRRLGAGGMSTVFLATDTVLERSVAVKLLAE
                     HLADDEDFVARFRREALAAARLQHPNVVQVFDSGLDGESHRHYIVMEFVDGPSAADML
                     REHKRLDLDEALNVIRDACHGLDYAHRAGVVHRDVKPGNLLVAAESHTTKLADFGIAK
                     ASQQTRITQVGSVLGTAAYLSPEQARGEEAGPASDIYSLGVCAYQFLAGRLPHEYSSL
                     TELALKQQEETVEPITVHRPEVPPELDRAIRVCLAREPEARYATTLEMAQALDAGLGG
                     DVTDATRMLAAAGTGIEELDATRALERTQALRRQPTHAPTPPPAYRRDPTGVQPAAEP
                     SKAEKRARRNKRLGGLFAFLAVVGAIAAVALAFNSSSSNNGPEPVDQGDVTQQVDGIK
                     QFLRENTR"
     gene            complement(1742..2293)
                     /locus_tag="VFY44_08465"
     CDS             complement(1742..2293)
                     /locus_tag="VFY44_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910514.1"
                     /translation="MTFHRRHLLAAAAACAVAVAGCGSSDEPEGEKLPQSTVSQLNVR
                     LDEIQRRFDAAQEQNLPGACDDIQNDSFPAVKRLIDGLPEDVDGQLRDATEESFARLQ
                     QLVDDGCQDIAPTETETETTETVPPETVPPETTPPETTPTETTPPETTPPEETPPEDE
                     GGNGGTDPDGTGGGGTQAPQGSG"
     gene            complement(2290..3111)
                     /locus_tag="VFY44_08470"
     CDS             complement(2290..3111)
                     /locus_tag="VFY44_08470"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX5910515.1"
                     /translation="MAAPAATAVATRATGRRQRGRAIPPDSIGDPLKIAVLSDVHGNL
                     PALRAVLEDVDAAGPDRVWCLGDLVGYGAHPDGCVQTARERCDVNLAGNHDLGVLDHI
                     DIADFAPHAEQAARWTRENASEETLRYLETLSPRHDGEQIGLFHASPRDPIWEYVLSG
                     TQAAECMDFMEPRVSAIGHTHVASYFSRTDGAMAGGTAAAGTELNIDAGEWLLNPGSV
                     GQPRDGDPRAAWLLLDVEQWTATWRRTEYPVDEAAQAINAAGLPTGLGERLYLGQ"
     gene            3215..4312
                     /locus_tag="VFY44_08475"
     CDS             3215..4312
                     /locus_tag="VFY44_08475"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX5910516.1"
                     /translation="MQRLIFIGQIGLSGATLAFLALRPPARFAQMLERLGARPVLGGA
                     AGGAALSLILAVVGLPLGYWAHERAVDVGLSTQSLGPWLGDQALTAAIGAVFGAIAGA
                     VFLGLVRRYPRRWFVPAAAAVVVIGVVSTYLTPVVIDPLFNKFDPLPKGPLRTEVLRL
                     AKAADVDVGQVYRVDASRRTTAINAYVGGIGHTKRVVIYDNLIEDYPRDQVRSVVAHE
                     LGHVHGRDVPRGLLWLALVALPGTFLAQVLAERIAPRELTPRRGARRAGPEALPALAL
                     SLALVSFGLTCAGNALSRRVEAHADAYALRLTDDPKAFIELERSLTLRNLGDPDPPAV
                     TQKLLGTHPTTVERIGFGRRWEQEGRRSLSP"
     gene            complement(4302..4628)
                     /locus_tag="VFY44_08480"
     CDS             complement(4302..4628)
                     /locus_tag="VFY44_08480"
                     /inference="COORDINATES: protein motif:HMM:NF023376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HEX5910517.1"
                     /translation="MPEQDDDRQFDIHADPEVMAGHYSNFANVSHSDYEFTVTFARVD
                     HEVDEDAVPGVVVSRINLSPRFMRELIDAMEDNFSKWQTREGIKNLPEWDDGKSQGEG
                     PGTPTG"
     gene            4665..>4855
                     /locus_tag="VFY44_08485"
     CDS             4665..>4855
                     /locus_tag="VFY44_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910518.1"
                     /translation="MRLVVIAVGRLRPPYADDVQHYKKLLAGHARLELVEVKDDSQVQ
                     RRIPDRAFTVLLDSGGRTFG"
BASE COUNT          586 a         1745 c         1792 g          732 t
ORIGIN      
        1 acctacgacg acctgcgcga cgcgtcggcg gagcgggtcg cctcgacgcc cggcctggac
       61 gggatcgcgg taggcggctc gctcgggcag gagaaggagc agatgcacgc ggtggtcgag
      121 cggtcgctcc ggcccgcccc ggacgagtgg ccgcgccatc tgctcggcat cggcgacgtc
      181 gacgtcatcc tcgcggcggt cagggcgggg atcgacacct tcgactgcgc cacgccgacg
      241 cggctggccc gccacggcac cgcgctggtg cccgaccccg agcggcgctg gaagatcgac
      301 ctgaccaagt ccgagtggcg cgacgacccg cggccgctgt tcgagggcgc cgccacgccc
      361 cattcacgcg cctacctgca ctacctggcc aagggccggg agctgaccgg cgcgcgcatc
      421 ctgacgctgc acaacctcga cttcatggcc cggctgatgt cgggcattcg cgccgcgatc
      481 cgcgacggca gctgggagca gtacgccgag aaggtgctgg cgggcgagga gccgtacggc
      541 tagcgggtgt tctcgcgcag gaactgcttg atgccgtcga cctgctgggt cacgtcgccc
      601 tggtcgaccg gctcgggccc gttgttggac gacgaggagt tgaacgccag ggccaccgcg
      661 gcgatggcgc ccacgacggc caggaacgcg aacaggccgc ccaggcgctt gtttcgcctg
      721 gcccgcttct cggccttcga gggctcggcc gcgggctgga ctccggtcgg gtcgcgccgg
      781 taggcggggg gcggggtggg ggcgtgcgtg ggctggcggc gcagcgcctg cgtgcgctcc
      841 agggcccggg tcgcatccag ctcctcgata cccgtgccgg ccgcggccag catgcgggtg
      901 gcgtcggtca cgtcgccgcc caggccggcg tccagcgcct gggccatctc cagcgtggtg
      961 gcgtagcggg cctcgggctc gcgcgccagg cacacgcgga tcgcgcggtc cagctccggc
     1021 gggacctcgg gacggtgcac ggtgatcggc tccaccgtct cctcctgctg cttcagcgcc
     1081 agctcggtca gcgacgagta ctcgtgcggc aggcgcccgg ccaggaactg gtaggcgcac
     1141 acccccagcg agtagatgtc cgaggccggg ccggcctcct cgccgcgggc ctgctcgggg
     1201 gagaggtagg cggcggtgcc cagcaccgag cccacctggg tgatccgcgt ctgctgcgag
     1261 gccttggcga tgccgaagtc ggccagcttg gtggtgtgcg actcggccgc caccagcagg
     1321 ttgccgggct tgacgtcgcg gtgcaccacg ccggcgcggt gggcgtagtc cagcccgtgg
     1381 cacgcgtccc ggatcacgtt cagcgcctcg tccaggtcca ggcgcttgtg ctcacgcagc
     1441 atgtcggccg ccgacgggcc gtccacgaac tccatgacga tgtagtggcg gtgcgactcg
     1501 ccgtccagcc ccgagtcgaa cacctggacc acgttcgggt gctggagccg ggccgcggcc
     1561 agcgcctcgc ggcggaagcg ggccacgaag tcctcgtcgt cggccaggtg ctcggccagc
     1621 agcttcacgg ccacggaccg ctcgagcacg gtgtcggtgg ccaggaacac ggtggacatg
     1681 ccgcccgcgc ccaggcggcg gtcgagcgcg taccggccgg cgatctcacc cggagcggcc
     1741 atcagcccga tccctgcggg gcctgggtgc cgccgccgcc ggtgccgtcg gggtcggtgc
     1801 cgccgttgcc accctcgtcc tcgggcgggg tctcctccgg cggggtggtc tccggcgggg
     1861 tggtctccgt gggggtggtc tcgggcggtg tggtctccgg gggcaccgtc tcgggcggca
     1921 cggtctcggt cgtctccgtc tcggtctcgg tcggcgcgat gtcctggcag ccgtcgtcga
     1981 ccagctgctg gaggcgggcg aagctctcct cggtggcgtc gcgcagctgg ccgtccacgt
     2041 cctcgggcag gccgtcgatc agtcgcttga ccgccgggaa cgagtcgttc tggatgtcgt
     2101 cgcaggcgcc gggcaggttc tgctcctggg cggcgtcgaa gcggcgctgg atctcgtcca
     2161 ggcgcacgtt cagctgcgac accgtgctct ggggcagctt ctcaccctcg ggctcgtccg
     2221 aggagccgca tccggcgacg gccacggcgc aggccgcggc ggcggccagg aggtgtcggc
     2281 ggtggaaggt cattgcccca agtagagccg ttcgcccaga ccggtgggca gacctgccgc
     2341 gttgatggcc tgcgcggcct catccacggg gtactcggtt cgtcgccagg tcgcggtcca
     2401 ctgctccacg tctagcagga gccaggccgc ccgtgggtca ccgtcgcgcg gctggcccac
     2461 gctgccgggg ttcagcagcc actccccggc gtcgatgttc agctccgtgc ccgccgccgc
     2521 ggtcccgccg gccatcgccc cgtcggtgcg gctgaagtac gaggccacgt gcgtgtggcc
     2581 gatggcggac acccgcggct ccatgaaatc catgcactcg gcggcctgcg tgccggacag
     2641 cacgtactcc cagatcggat cgcgcggcga ggcgtggaag aggccgatct gctcgccgtc
     2701 gtgcctgggg ctcagcgtct ccaggtaacg cagcgtctcc tcggacgcgt tctcgcgcgt
     2761 ccagcgcgcc gcctgctcgg cgtggggggc gaagtcggcg atgtcgatgt ggtccagcac
     2821 ccccaggtcg tggttgccgg ccaggttcac gtcgcagcgc tcgcgggccg tctggacgca
     2881 cccgtcgggg tgtgcgccgt agcccaccag gtcgcccagg caccagaccc ggtccgggcc
     2941 ggcggcgtcc acgtcctcga gcacggccct gagggccggg aggttgccgt ggacatcaga
     3001 gagaaccgcg atcttcaggg ggtcaccgat actgtccggg ggaatggctc gtcctcgctg
     3061 gcgcctaccg gtcgctctgg tggccacagc ggtcgccgcg ggggccgcca cgctgctcct
     3121 taggccccgg agcggcctga tcgacccagc cgcagtcgac gcgacagcct acttcagccc
     3181 ggcccaactg gagcgcgccg acgatttccg ttcattgcag cggctcatct tcatcggcca
     3241 gatcggcctc tccggggcca cgctggcctt cctggcgttg cggcccccgg cgcggttcgc
     3301 ccagatgctg gagcgcctgg gcgcccggcc ggtgctgggc ggggcggcgg gcggggcggc
     3361 cctgtcgctg atcctggccg tggtggggct gcccctgggc tactgggccc acgagcgcgc
     3421 ggtggacgtg ggcctctcga cacagtcgct gggcccgtgg ctgggcgacc aggcgctcac
     3481 ggcggccatc ggcgcggtgt tcggcgccat cgccggcgcg gtgttcctgg gcctggtgcg
     3541 ccgctacccc cgccgctggt tcgtccccgc ggccgcggcg gtggtggtca tcggcgtggt
     3601 gtccacctac ctcacgccgg tggtcatcga ccccctgttc aacaagttcg acccgctgcc
     3661 gaaggggccg ctgcgcaccg aggtgctgcg cctggccaag gcggccgacg tggacgtggg
     3721 gcaggtctac cgggtggacg ccagccgccg caccaccgcc atcaacgcgt acgtgggcgg
     3781 gatcggccac accaagcggg tggtcatcta cgacaacctg atcgaggact acccgcgcga
     3841 ccaggtccgc agtgtggtgg cgcacgagct gggccacgtg cacggccggg acgtgccgcg
     3901 cggcctgctg tggctcgccc tcgttgcgct tcccggcacg ttcctggccc aggtcctggc
     3961 ggaacggatc gcaccccgcg agctgacgcc gcgccggggc gcccgcaggg cgggccccga
     4021 ggcgctgccc gccctggcgc tctcgctggc cctggtcagc ttcgggctga cgtgcgccgg
     4081 caacgcgctg tcacgccgcg tcgaggcgca cgccgacgcc tacgcgctgc ggttgaccga
     4141 cgacccgaag gcgttcatcg agctggagcg cagcctgacg ctgcgcaacc tgggcgatcc
     4201 cgacccgccc gcggtgacgc agaagctgct gggcacccac ccgaccacgg tcgagcggat
     4261 cggcttcggc aggcgctggg agcaggaggg gaggcggtcc ctcagcccgt aggggtgccg
     4321 gggccctcgc cctggctctt gccgtcgtcc cactcgggca ggttcttgat gccctcgcgg
     4381 gtctgccact tggagaagtt gtcctccatg gcgtcgatca gctcgcgcat gaagcgcggc
     4441 gacaggttga tccgcgagac caccacgccc ggcacggcgt cctcgtccac ctcgtggtcc
     4501 acgcgcgcga aggtgaccgt gaactcgtag tcggagtggc tcacgttggc gaagttcgag
     4561 tagtggccgg ccatcacctc ggggtcggcg tggatgtcga actgtcggtc gtcgtcttgc
     4621 tctggcacgg ttcggagtat cgcacccccg cgcacgcccg ccccatgagg ctcgtcgtga
     4681 tcgccgtggg ccggctccgg ccgccctacg cggacgacgt ccagcactac aagaagctcc
     4741 tggccggcca cgcgcggctg gagctcgtcg aggtcaagga cgacagccag gtccagcgcc
     4801 gcatccccga ccgcgcgttc acggtgctgc tggactccgg cggtcgcacc ttcgg
//
LOCUS       DASUPW010000377         5426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_87638, whole genome shotgun sequence.
ACCESSION   DASUPW010000377 DASUPW010000000
VERSION     DASUPW010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87638"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VFY44_08490"
     CDS             complement(<1..470)
                     /locus_tag="VFY44_08490"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5910519.1"
                     /translation="MTDQAKVIAFTNQKGGVAKTTTTLNLAAAFVESGHRALCVDLDP
                     QGNLTMSQGIDPDTLEVSMYDVLVHDMSIREVIRKREVDVACASIDLAGAEIAMSTKI
                     GRERSLEKALRPIKEDYDFICIDTPPSLGLLTINALTASDKVIVPVQCEYLSMRG"
     gene            569..1501
                     /locus_tag="VFY44_08495"
     CDS             569..1501
                     /locus_tag="VFY44_08495"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HEX5910520.1"
                     /translation="MPASPLHKRLVFVTGKGGVGKSTVAAAIGLAGAEAGRRTIIAEV
                     SEQERITGLFGKPAAGYRETEVAHNLSAFSLNPEDAKEEWLRDQLKSGRLAGMLNNSR
                     IFSYLTAAAPGLDELVTLGKIWELAQMERRKKSDSAFDTCVVDAPASGHGIAMLRSPR
                     TYADIAKVGPVARHATFFDTYIRDGRSTAVIAVALAEEMPVNETIDLERRLKDEVGVK
                     LAAVVVNAVLPERYSNAEAEQLQGVDGQGGPATREAVATALAAHTRAKEQHSQIARLK
                     RALDAPVTTLPFLLTPEVDVDGLRELATELGRKL"
     gene            1498..2586
                     /locus_tag="VFY44_08500"
     CDS             1498..2586
                     /locus_tag="VFY44_08500"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEX5910521.1"
                     /translation="MTAVAGVEELIGRKQVVICAGSGGVGKTTTSAAIAAGMADRGKK
                     AAVLTIDPARRLANSLGLSELGNEEARVELPGASGELWAMMLDAKRTFDELIDTYAPD
                     EETRDSVLANPIYKQLSNAVAGSQEYMAMEKLHELHQEGRYDLLVLDTPPSRNALDFL
                     EAPRRLSRFIDSRSLRFFQSSSRMSLGLMGKGTGVLFSIMKRATGVDLLRDLSAFFNA
                     FGSMTDGFSERARHVEALLGDSRTTFLLVTSPREASIREAEWFAKRLREEDLPFGGVV
                     VNRVRREIGTSPSPQLDTELTGLLGDKLGAKVSRTLGEQVALAEVDAHNLEDLTRRLG
                     RKPTLAVPELASDVHDLDGLRALNDHLF"
     gene            complement(2557..4179)
                     /locus_tag="VFY44_08505"
     CDS             complement(2557..4179)
                     /locus_tag="VFY44_08505"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5910522.1"
                     /translation="MARTLAFLFFAGGSTVLLSLAAGHAPDTDARGVVVTAILALVVG
                     PVLLAVGRRLPDWGVPLFLVCGTTAITSVVYFDGQGSIYAAFYPWVGAEAFLFLNRRG
                     ILLQIVYMATAYAWALHATDTTNAWQHWCVAMGATIALGLLVGYLRLRLVRLLDRLTA
                     VARTDELTAIPNRRAFQERFEQELARAARDAQPLSVLMGDLDGFKLVNDRGGHHTGDA
                     ALRRVATELEQWKRLTDVAARVGGEEFALLLPATAHDEALQVAERLRLGIREVFAGDE
                     VPLSISFGIATFPIHAPDGPGVLRAADQALYSAKEQGRDRSVVFSEQASKSLEGARQR
                     LADSSEMQLATVVGLAEALDIRDTGTGQHSRTVARYAGMMARRMGFAGQRIERIELAG
                     LLHDVGKIGISDNVLTKPGPLDDDEWKQMRTHPQIGARLLGRPELADLRGWIVAHHER
                     PDGKGYPFGLSHGDIPLEARILSVADAYEAMTADRVYKRAMAAEAARGELLACAGTQF
                     DAEVVSVFLQALEEEEIEEDTSSSEEVVVQRT"
     gene            complement(4291..>5426)
                     /locus_tag="VFY44_08510"
     CDS             complement(4291..>5426)
                     /locus_tag="VFY44_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910523.1"
                     /translation="SAYLRLNGLRGAEAEPALRALLERLYDELSDFAFPEERFERLYA
                     EVEGTLYEKSQSATVLVPVHGLEMQGDRVDLGDGLSLVRGDRTDAPDEAVWGGEDDEP
                     SALLMLEREVAPDDPMPLAEAREQFRSLLHGMRLFKPGGIALGAVAWRRMGDGRWAPT
                     ELEPTGEARGVPWILVEGEEAELSRFLDAVASPPRTGPVAWSLSRFQMGAGRRLEAEA
                     LTDYLLALRALIDEEGSSLALRVAVLCAEESERRRVQRRVELAQAMERFVIGDGADQD
                     YMDAVGTDSPRTLVEEVERHLRALLRDILCGYLEADLRHVADELLLDQPEPFEIRAAS
                     LKPEPVSEPAPEPVPIRTPVQDEPVQLEGQLGLDDAEYWSAPV"
BASE COUNT          773 a         1935 c         1975 g          743 t
ORIGIN      
        1 cccctcatcg acagatactc gcattgcacg gggacgatca ccttgtcgga ggccgtcagg
       61 gcgttgatcg tgagcagccc gagcgacggc ggggtgtcga tgcagatgaa gtcgtagtcc
      121 tccttgatcg ggcgcagcgc cttctccagc gagcgctccc ggccgatctt ggtggacatg
      181 gcgatctcgg cgccggccag gtcgatcgaa gcgcaggcca cgtcgacctc gcgcttgcgg
      241 atgacctcgc ggatggacat gtcgtgcacg agcacgtcgt acatcgacac ctcgagcgtg
      301 tccgggtcga tgccctggct catggtcagg ttgccctggg ggtccaggtc cacgcacagg
      361 gcgcggtgac cgctctcgac gaacgcggcg gccagattga gggtcgtggt ggtcttggcc
      421 accccgccct tctggttggt gaaggcgatc accttcgcct ggtcggtcac gggcgagctc
      481 ctccgctgaa ggctgcatag ggcattacga ggggcttatt cgcataagcg tgggcaactc
      541 cttccgtctc gcacgtaggg tctgtgcaat gcccgcctcc cccctgcaca agcgcctggt
      601 cttcgtgacc ggcaagggcg gggtcggcaa gagcaccgtg gccgccgcga tcgggctggc
      661 cggcgccgag gccggccgcc gcacgatcat cgccgaggtg tccgagcagg agcgcatcac
      721 cggcctgttc ggcaagcccg cggcgggcta ccgggagacc gaggtggcgc acaacctgtc
      781 ggcgttctcg ctgaaccccg aggacgccaa ggaggagtgg ctgcgcgacc agctgaagtc
      841 gggccgcctg gccgggatgc tcaacaacag ccgcatcttc tcctacctga ccgcggccgc
      901 ccccggcctg gacgagctgg tcacgctggg caagatctgg gagctggccc agatggagcg
      961 gcgcaagaag agcgacagcg cgttcgacac ctgcgtggtg gacgcgcccg ccagcgggca
     1021 cgggatcgcg atgctgcgca gcccacgcac gtacgccgat atcgccaagg tcggcccggt
     1081 ggcgcggcac gcgacgttct tcgacaccta catccgcgac gggcgctcga ccgcggtgat
     1141 cgccgtggcg ctggccgagg agatgccggt gaacgagacc atcgacctgg agcgccggct
     1201 caaggacgag gtcggggtca agctggccgc ggtggtggtc aacgccgtgc tgcccgagcg
     1261 ctactcgaac gccgaggccg agcagctcca gggcgtggac ggccagggcg gccccgcgac
     1321 gcgcgaggcg gtggccaccg cgctggccgc gcacacgcgc gcgaaggagc agcactccca
     1381 gatcgcccgc ctcaagcgcg cgctggacgc tcccgtgacc acgctgccgt tcctgctgac
     1441 gcccgaggtc gacgtggacg ggctgcgcga gctggccacc gagctggggc gcaagctgtg
     1501 accgcggtgg cgggggtcga ggagctgatc ggccgcaagc aggtggtcat ctgcgcgggc
     1561 tcgggcggcg tgggcaagac cacgacctcg gcggccatcg ccgcgggcat ggccgaccgg
     1621 ggcaagaagg cggccgtgct gaccatcgac cccgcgcggc ggctggccaa ctcgctgggc
     1681 ctgagcgagc tgggcaacga ggaggcgcgg gtcgagctgc ccggcgccag cggtgagctg
     1741 tgggcgatga tgctggacgc caagcgcacg ttcgacgagc tgatcgacac ctacgcgccg
     1801 gacgaggaga cgcgcgactc ggtgctggcg aacccgatct acaagcagct gtcgaacgcc
     1861 gtcgcgggct cgcaggagta catggccatg gagaagctgc acgagctcca ccaggagggc
     1921 cgctacgacc tgctggtgct ggacacgccg cccagccgca acgcgctcga cttcctggag
     1981 gcgccgcggc gcctgtcgcg cttcatcgac tcgcgctcgc tgcgcttctt ccagagctcc
     2041 agccggatga gcctcggcct gatgggcaag ggcaccggcg tgctgttctc gatcatgaag
     2101 cgggccacgg gcgtggacct gctgcgcgac ctgtcggcct tcttcaacgc cttcggcagc
     2161 atgaccgacg gcttctccga gcgcgcccgc cacgtggagg cgctgctggg cgacagccgc
     2221 acgacgttcc tgctggtgac ctcgccgcgc gaggcctcga tccgcgaggc cgagtggttc
     2281 gccaagcgcc tgcgcgagga ggacctgccg ttcggcggcg tggtcgtcaa ccgggtgcgg
     2341 cgggagatcg gcaccagccc ctcgccgcag ctggacaccg agctgaccgg gctgctgggc
     2401 gacaagctcg gcgcgaaggt ctcgcgcacg ctgggcgagc aggtggccct ggccgaggtc
     2461 gacgcccaca acctggagga cctgacgcgg cggctggggc gcaagcccac gctggccgtg
     2521 cccgagctgg cgagcgacgt gcacgacctg gacgggctac gtgcgctgaa cgaccacctc
     2581 ttctgaggag ctggtgtcct cctcgatctc ctcctcctcc agggcctgga ggaagacgct
     2641 gaccacctcg gcgtcgaact gcgtgcccgc gcaggccagc agctccccgc gggcggcctc
     2701 ggccgccatc gcgcgcttgt agacgcggtc ggcggtcatg gcctcgtagg cgtcggccac
     2761 cgagaggatc cgggcctcga gcgggatgtc gccgtgggac aggccgaacg ggtagccctt
     2821 gccgtcgggg cgctcgtggt gggccacgat ccagccacgc aggtcggcca gctcgggccg
     2881 gccgagcagc cgggcgccga tctgcggatg cgtgcgcatc tgcttccact cgtcgtcgtc
     2941 cagcgggccg ggcttggtca gcacgttgtc cgagatgccg atcttgccca cgtcgtgcag
     3001 cagcccggcc agctcgatcc gctcgatccg ctggccggcg aagcccatgc ggcgggccat
     3061 catgcccgcg tagcgcgcca ccgtgcgcga gtgctggccc gtgccggtgt cgcggatgtc
     3121 cagggcctcg gccaggccga ccaccgtggc cagctgcatc tcgctgctgt cggccaggcg
     3181 ctggcgcgcc ccctccagcg acttcgaggc ctgctcgctg aacaccaccg agcggtcgcg
     3241 gccctgctcc ttggcgctgt acagcgcctg gtcggcggcc cgcagcaccc cgggcccgtc
     3301 gggcgcgtgg atggggaagg tggcgatgcc gaagctgatg ctcagcggga cctcgtcgcc
     3361 ggcgaagacc tcgcggatgc caagcctcag ccgctcggcc acctgcaggg cctcgtcgtg
     3421 ggcggtggcg ggcagcagga gcgcgaactc ctcgccgccc acgcgggcgg ccacgtcggt
     3481 caggcgcttc cactgctcca gctccgtggc cacccgccgc agcgcggcgt cgccggtgtg
     3541 gtggccgccg cggtcgttga ccagcttgaa gccgtcgagg tcgcccatca gcacgctcag
     3601 cggctgggcg tcgcgggccg cgcgggccag ctcctgctcg aagcgctcct ggaacgcgcg
     3661 ccggttcggg atcgcggtga gctcgtcggt gcgggccacc gcggtcaggc ggtccagcag
     3721 gcgcacgagg cgcaggcgca ggtatcccac cagcaggccg agcgcgatgg tggcgcccat
     3781 cgccacgcac cagtgctgcc acgcgttggt cgtgtcggtg gcgtgcagcg cccaggcgta
     3841 ggcggtggcc atgtagacga tctggagcag gatcccgcgc cggttcagga acaggaacgc
     3901 ctccgcgccg acccacgggt agaacgcggc gtagatgctg ccctgcccgt cgaagtagac
     3961 gaccgaggtg atcgcggtgg tgccgcagac caggaacagc ggcacgcccc agtcgggcag
     4021 ccgccggccc accgccagca gcaccgggcc cacgaccagc gccaggatcg cggtgaccac
     4081 cacgccgcgg gcgtcggtgt cgggcgcgtg cccggcggcc agcgagagca gcacggtcga
     4141 gccgccggcg aagaacagga acgccagggt gcgggccatc tcctcctgac cgaagggatg
     4201 ctctacgccc tgatgcccgg ggacgggggc cagccgcata tctgccctat cgggcagggg
     4261 gcgctggtcc tttagggtcg gtcgatggcc ctataccggg gccgaccagt actccgcgtc
     4321 gtccagtccg agctggccct ccagctgaac cggctcgtcc tggacgggtg tccggatcgg
     4381 cacgggctcc ggcgcgggct ccgagaccgg ctcgggcttg agcgacgcgg ccctgatctc
     4441 gaacggctcg ggctggtcga gcaggagctc gtcggccacg tggcgcaggt cggcctcgag
     4501 gtagccgcag aggatgtcgc gcagcagggc gcgcaggtgg cgctcgacct cctcgaccag
     4561 cgtgcgcggc gagtcggtgc ccacggcgtc catgtagtcc tggtcggcgc cgtcgccgat
     4621 cacgaaccgc tccatcgcct gggccagctc gacgcggcgc tgcaccctcc ggcgctcgct
     4681 ctcctcggcg cacagcaccg ccacgcgcag cgccaggctc gacccttcct cgtcgatcag
     4741 cgcgcgcagg gccagcaggt agtcggtcag ggcctcggcc tcgagccggc ggccggcgcc
     4801 catctggaac cgcgacagcg accaggccac gggcccggtg cgcggcggtg aggccacggc
     4861 gtccaggaag cgcgacagct cggcctcctc gccctcgacc aggatccacg gcaccccgcg
     4921 cgcctcgccg gtcggctcga gctcggtggg agcccagcgg ccgtcgccca tccggcgcca
     4981 cgccaccgcc cccagcgcga tcccgccggg cttgaacagg cgcatgccgt gcagcagcga
     5041 ccggaactgc tcgcgcgcct ccgccagcgg catcgggtcg tcgggcgcga cctcgcgctc
     5101 cagcatcagc agggcgctgg gctcgtcgtc ctccccgccc cagacggcct cgtcgggggc
     5161 gtcggtccgg tcgccgcgga ccagcgacag gccgtcgccc aggtcgaccc ggtcgccctg
     5221 catctccagg ccgtgcacgg gcaccagcac ggtggccgac tggctcttct cgtacagggt
     5281 cccttcgacc tcggcgtaga ggcgctcgaa gcgctcctcg gggaaggcga agtccgacag
     5341 ctcgtcgtag aggcgctcga gcagggcgcg cagggcgggc tcggcctcgg cgccgcggag
     5401 cccgttcaga cgcaggtagg cggacg
//
LOCUS       DASUPW010000378         3384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1057530, whole genome shotgun sequence.
ACCESSION   DASUPW010000378 DASUPW010000000
VERSION     DASUPW010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057530"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1596)
                     /locus_tag="VFY44_08515"
     CDS             complement(<1..1596)
                     /locus_tag="VFY44_08515"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX5910524.1"
                     /translation="MDDQNRTWRRLFAAMVLAASIVVIAVVPSLASPKAGGRELLPDL
                     DQRVPTGVTVAASSTGWDLNFDSAVENVGDGPLQLQGSRASLAVGTMDATQQVITDTG
                     ETVEDANSGTLRYVRSPDHQHWHLDGFDRYELRKAVSGDLVGPDRKTGFCLGDRYDAD
                     PGGSLPGKPAQPVLREQCERNNPDALEVVSGISVGYGDEYRGGLEGQSIDVTGVPAGR
                     YVLVHRVNADGKLREETDANNAASVLIQLSWPRGTGAEPEVSVLARCPDTATCRATPA
                     AGSTGPATEATVIAGGLDTPWEVLPLPDGRVLVTEQPGRVRVISPSGVLQAGALHTEP
                     GATKFLGMARDPGYAYNRSVYLYVTRPEGSRVVRLTDTGTTLANPTTIFPAGGAPPIA
                     SDGRHDGGRIAFGPDGKLYVTTGDIHDPDLPQDTTSPNGKILRMNADGTAPGDNPFAG
                     GTGAAPWVWSYGHRHPQGIAWDSQGRMWESEHGPSGEAHAPAGMKTGNDEINRIEAGR
                     DYGWPLVAGDQTAAGMTPPVAHAG"
     gene            complement(1645..2781)
                     /locus_tag="VFY44_08520"
     CDS             complement(1645..2781)
                     /locus_tag="VFY44_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5910525.1"
                     /translation="MATNPFRYGALALDDSFTNRENELAELTADVSNGQDVVIFAPRR
                     YGKSSLVWRASQELVAGHDVLVAQVDLMSTPTKEKLAEKLAQAIYEELASVLFKAKER
                     MRVFQGLRVRPTVTVDPDDGTMSFSFAASRAPEDLDATLERLFELPGRLASERGRKVA
                     LVLDEFQEIVDIDRGLPKLMRSVFQAQPEVAHVYLGSKRHMMEKIFSDEQEPFWRSAK
                     RIELGVIEPAKFRPFIRHGFRSTGRTIEPHALDRVLEVTGGHPYATQELAYFLWQETP
                     EGEEADDARLDIALGRVLSSENAHFGLIWERASGAQRQLLQALAAEPGRPLTNNYRRF
                     HSLPGPSSVQRALETLEKQELVARSGGDSWIAEPFFAEWVRRLN"
     gene            complement(2833..>3384)
                     /locus_tag="VFY44_08525"
     CDS             complement(2833..>3384)
                     /locus_tag="VFY44_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5910526.1"
                     /translation="DLSLGVAESSMLRVRSHPGEAQRELRDHDERFYVVRPRPLSLGQ
                     TQSLPVPGRLELGDQELELYATGGHTSDGMAVFAPWAGVLVVGDYLSPVEIPMISPGG
                     SVADYRGTLAALAPVVERAGSVVPGHGRVLDRGDAQRLIEEDLAYLDALEGGGERVTL
                     PEGRAGGEQQRIHAENLRATAAA"
BASE COUNT          440 a         1245 c         1194 g          505 t
ORIGIN      
        1 gcccgcgtgt gccaccggag gtgtcatgcc ggccgcggtc tggtcacccg cgaccagcgg
       61 ccagccgtag tcccggccgg cctcgatccg gttgatctcg tcgttgccgg tcttcatccc
      121 ggccggagca tgcgcctcgc cgctggggcc gtgctcggac tcccacatgc gtccctggga
      181 gtcccacgcg atgccctgcg gatggcggtg cccgtagctc cacacccacg gcgccgctcc
      241 ggtcccgccc gcgaacgggt tgtcgcccgg cgccgtgccg tcggcgttca tccggaggat
      301 cttgccgttc ggcgatgtcg tgtcctgggg cagatccggg tcgtggatgt cgcccgtggt
      361 cacgtacagc ttgccgtccg gcccgaaggc gatgcgcccg ccgtcgtggc gcccatcgct
      421 ggcgatcggc ggcgcgccgc cggcggggaa gatcgtggtc gggttcgcga gggtggtgcc
      481 ggtgtcggtc agccgcacga cccggctgcc ctcgggcctg gtcacgtaca ggtagacgct
      541 gcggttgtag gcgtagccgg gatcccgcgc catgcccagg aacttcgtgg cccccggctc
      601 ggtgtgcagg gctcctgcct gcaacacgcc ggacgggctg atcacccgga cccggccggg
      661 ctgctcggtg accaggaccc ggccgtcggg caacggcagc acctcccacg gggtgtccag
      721 accgccggcg atcacggttg cctcggtggc gggaccggtc gagccggccg cgggcgtggc
      781 cctgcacgtg gcggtgtccg ggcaccgcgc gagcaccgac acttcgggct ccgccccggt
      841 gccccgcggc cagctgagct gaatcagcac ggacgccgcg ttgttggcgt ccgtctcctc
      901 ccgcagcttg ccgtcggcgt tgacccggtg gaccagcacg tagcgcccgg ccggaacgcc
      961 cgtcacgtcg atggactggc cctccagacc gccccggtac tcgtcgccgt accccaccga
     1021 gatgcccgag accacctcca gcgcatcggg gttgttgcgc tcgcactgct cgcgcagcac
     1081 gggctgggcg ggctttcccg gcagcgatcc cccggggtcg gcgtcgtagc ggtcaccgag
     1141 gcagaagccg gtcttgcggt ccgggccgac caggtcgccg gacaccgcct tccgtagctc
     1201 gtagcggtcg aagccatcga ggtgccagtg ctggtggtcg ggggacctga cgtagcgcag
     1261 ggtgcccgag ttggcgtctt cgacggtctc gccggtgtcc gtgatcacct gctgggtggc
     1321 gtccatggtg cccaccgcca gcgaggcccg ggagccctgc aactgcagcg ggccgtcgcc
     1381 cacgttctcc acggcggagt cgaagttcag gtcccacccc gtgctgctcg ctgccacggt
     1441 gaccccggtc gggacccgct ggtccaggtc gggcaacagc tcccggccgc cggctttcgg
     1501 actggccagc gacggcacca cggcgatcac cacgatcgag gcggcgagca ccatggcggc
     1561 gaacagtcgc ctccatgtcc ggttctggtc gtccatggcc aggagacgag tatgtgggcg
     1621 atacccccgg gtcgccagag ggcgctagtt cagccgccgc acccattcgg cgaagaaggg
     1681 ctcggcgatc caggagtcgc cgccgctgcg ggccaccagc tcctgcttct cgagcgtctc
     1741 gagcgcgcgc tggaccgagg acggccccgg cagcgagtgg aagcggcggt agttgttggt
     1801 cagcgggcgc cccggctcgg cggccagggc ctgcagcagc tggcgctggg cgcccgatgc
     1861 gcgctcccag atcaggccga agtgcgcgtt ctcggacgac aggacccggc ccagcgcgat
     1921 gtccaggcgg gcgtcgtcgg cctcctcgcc ctcgggcgtc tcctgccaca ggaagtaggc
     1981 cagctcctgg gtcgcgtacg ggtggccgcc ggtgacctcg agcacgcggt ccagcgcgtg
     2041 gggctcgatc gtgcggccgg tggagcggaa cccgtggcgg atgaacgggc ggaacttcgc
     2101 cggctcgatc acgccgagct cgatccgctt ggcgctgcgc cagaacggct cctgctcgtc
     2161 ggagaagatc ttctccatca tgtggcgctt gctgcccagg tagacgtggg cgacctcggg
     2221 ctgggcctgg aagaccgacc gcatcagctt gggcaggccg cggtcgatgt ccacgatctc
     2281 ctggaactcg tccagcacca gcgcgacctt gcggccacgc tcggaggcca gccgcccggg
     2341 cagctcgaac aggcgctcca gcgtggcgtc caggtcctcg ggcgcgcgtg aggcggcgaa
     2401 cgagaacgac atcgtgccgt cgtcgggatc gaccgtgacc gtgggcctga cgcgcaggcc
     2461 ctggaagacc cgcatgcgct ccttggcctt gaacagcacc gaggccagct cctcgtagat
     2521 ggcctgggcc agcttctcgg ccagcttctc cttggtgggg gtggacatca gatccacctg
     2581 cgccaccagc acgtcgtgcc cggcgaccag ctcctgggag gcccgccaca ccagcgacga
     2641 cttgccgtac ctccgcgggg cgaagatgac cacgtcctgg ccgttggaca cgtccgccgt
     2701 cagctcggcg agctcgttct cgcggttggt gaacgagtcg tccagcgcca gcgcgccgta
     2761 ccggaagggg ttcgtggcca tcggaggcct agcgtagcag cgttacgcga tcttgcgtaa
     2821 cgcggatgtg cgttacgcag ccgccgtggc gcgcaggttc tcggcgtgga tccgctgctg
     2881 ctcgccgccc gcccgcccct cgggcagcgt cacacgctcc ccgccgccct ccagcgcgtc
     2941 caggtaggcc aggtcctcct cgatcaaccg ctgcgcgtcg ccgcggtcca gcacccgccc
     3001 atggccgggg accacgctgc cggcgcgctc gaccaccggg gccagcgcgg ccagggtgcc
     3061 gcggtagtcg gccaccgagc cgcccggcga gatcatcggg atctccaccg gcgacaggta
     3121 gtcgcccacc accagcacgc cggcccaggg ggcgaacacc gccatcccgt cggacgtgtg
     3181 cccgccggtg gcgtacagct ccagctcctg gtcccccagc tccagccgcc cgggcaccgg
     3241 cagcgactgg gtctggccca gcgacagcgg ccgcggcctg accacgtaga agcgctcgtc
     3301 gtggtcgcgc agctcgcgct gggcctcgcc cgggtgtgag cgcacgcgca gcatgctcga
     3361 ctccgccacc cccagcgaca ggtc
//
LOCUS       DASUPW010000379         2197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_920073, whole genome shotgun sequence.
ACCESSION   DASUPW010000379 DASUPW010000000
VERSION     DASUPW010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920073"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1545)
                     /gene="tig"
                     /locus_tag="VFY44_08530"
     CDS             complement(139..1545)
                     /gene="tig"
                     /locus_tag="VFY44_08530"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX5910527.1"
                     /translation="MSAVKTDTEELENSRVRVSVEVDSQDLETHLATAAGQIGRDMRV
                     PGFRKGKVPPQVVLQQVGREAVLDEAVRSGLPEWYEAAVHSAGLAVVGSPDLNLSDLP
                     EKGDPLTFSFEVAVRPQATLGDYKGVEAPKREPEVSEDDVEAEIGRLRESLASLETVE
                     KAAENGDYVVIDFVGTVDGEPFEGGDARGFLLELGSDRLVPGFEEQLIGVSAGEERTV
                     EVTFPEDYQADHLAAKEAAFAVEVKEVKSKQLPDLDDDLAVEAGGFDTLDELREDLRK
                     RLGEAQEEAIDRDWREAAVDAAAANATVEIPHDLVHSKAHDMWHQTAHRLQHQGLDPQ
                     QYLQFVNKTEEELVTETEPDAERALRREAVLAAVIEAEKIEVEDDEILDTLRQSAAAE
                     SGGKEPSDKALRRSLEKARKRGQDDALREDIAMRKAVDVIVDSAKPVAAPKEEADSEL
                     WTPEDDKPEKKEIWTPEG"
     gene            1574..1726
                     /locus_tag="VFY44_08535"
     CDS             1574..1726
                     /locus_tag="VFY44_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910528.1"
                     /translation="MPRPPVPLRRGPLERARAWLVCGPLGHLWSAVADLAVFGIRSAL
                     KRAGLR"
     gene            1731..2129
                     /locus_tag="VFY44_08540"
     CDS             1731..2129
                     /locus_tag="VFY44_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910529.1"
                     /translation="MLNPLLLPQAVIDGLAVLPALLQQAQEANRTLTDAIDRADRISA
                     QADQLIAQADLVQESFLRAQATLELMVERGDELLNTASDARTGLEGAQAELARANDQV
                     AKIIEMGGPLERGVDRLDRIVSRVRRDEGS"
BASE COUNT          269 a          804 c          787 g          337 t
ORIGIN      
        1 gagggggtta cagcaggccc tcgacagcaa cagcgcggtg accgaacggc cccgcctccg
       61 ggcctaccca gcccgagccg tggggccgat cgactggtgc ggcggacagc aagagcgcgg
      121 ctctgaccgg cgcgcccctc agccctccgg cgtccagatc tccttcttct ccggcttgtc
      181 gtcctccgga gtccagagct cgctgtcggc ctcctccttg ggggccgcca ccggcttggc
      241 gctgtcgacg atcacgtcga ccgccttgcg catggcgatg tcctcgcgca gcgcgtcgtc
      301 ctggccgcgc ttgcgggcct tctcgagcga gcggcgcagg gccttgtccg agggctcctt
      361 gccgcccgac tcggccgcgg ccgactggcg cagggtgtcc aggatctcgt cgtcctcgac
      421 ctcgatcttc tcggcctcga tcacggcggc caggacggcc tcgcggcgca gggcgcgctc
      481 ggcgtcgggc tcggtctcgg tgaccagctc ctcctcggtc ttgttcacga actggaggta
      541 ctgctggggg tccaggccct ggtgctggag gcggtgcgcg gtctggtgcc acatgtcgtg
      601 cgccttggag tgcacgaggt cgtgggggat ctcgacggtg gcgttggcgg cggcagcgtc
      661 cacggcggcc tcgcgccagt cgcggtcgat ggcctcctcc tgggcctcgc ccaggcgctt
      721 gcgcaggtcc tcgcgcagct cgtccagggt gtcgaagccg ccggcctcga cggccaggtc
      781 gtcgtccagg tccggcagct gcttggactt gacctccttg acctcgacgg cgaaggcggc
      841 ctccttggcc gccaggtggt cggcctggta gtcctcgggg aaggtcacct cgacggtgcg
      901 ctcctcgccc gccgagacgc cgatgagctg ctcctcgaag ccgggcacga gccggtcgga
      961 gcccagctcc agcaggaagc cgcgggcatc gccgccctcg aacggctcgc cgtcgaccgt
     1021 gcccacgaag tcgatgacca cgtagtcgcc gttctcggcc gccttctcga cggtctcgag
     1081 cgaggcgagg gactcgcgca ggcgcccgat ctcggcctcg acgtcgtcct cggacacctc
     1141 gggctcgcgc ttgggggcct cgacgccctt gtagtcgccc agcgtggcct gggggcgcac
     1201 ggccacctcg aaggagaacg tcagcggatc gcccttctcg ggcaggtcgg agaggttgag
     1261 gtcggggctg ccgaccacgg cgaggccggc ggagtgcacc gcggcctcgt accactcggg
     1321 cagcccactg cggaccgcct cgtccagcac ggcctcgcgg ccgacctgct ggagcaccac
     1381 ctgcggcggc accttgccct tgcggaaccc gggcacgcgc atgtcgcggc cgatctgccc
     1441 cgcggcggtg gccaggtgcg tctccaggtc ctgtgaatcg acctcgacgc tgacgcggac
     1501 gcgggagttc tcgagctctt cggtatcggt cttgacggcg ctcatgacga acgccggacg
     1561 atagcgttca ggtgtgcccc gaccgcctgt gcccctgcgc cgcggcccgc tcgagcgcgc
     1621 ccgcgcgtgg ctggtgtgcg gccccctggg ccacctctgg agcgcggtgg ccgacctggc
     1681 cgtgttcggg atccggtccg cgctcaagcg cgcgggctta cgatgacccc gtgctgaacc
     1741 cgctcctgct cccgcaggcc gtgatcgacg gcctggccgt gctgccggcc ctgctccagc
     1801 aggcccagga ggccaaccgg acgctgaccg acgccatcga ccgcgcggac cgcatcagcg
     1861 cccaggccga ccagctgatc gcccaggcgg acctggtcca ggagtccttc ctgcgcgccc
     1921 aggcgacgct ggagttgatg gtcgagcgcg gcgacgagct gctgaacacc gcctcggacg
     1981 cgcggacggg actcgagggc gcgcaggccg agctggcccg cgcgaacgac caggtggcga
     2041 agatcatcga gatgggcggc ccgctggagc gcggcgtcga caggctggac cgcatcgtct
     2101 cgcgcgtgcg acgggacgag ggctcgtgat ccaggcgctc gccgaccgcg ccgccgcgag
     2161 cgggcggctg gccatcgaca ccgagttcgt gtccgag
//
LOCUS       DASUPW010000380         3840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_505547, whole genome shotgun sequence.
ACCESSION   DASUPW010000380 DASUPW010000000
VERSION     DASUPW010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505547"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VFY44_08545"
     CDS             complement(<1..356)
                     /locus_tag="VFY44_08545"
                     /inference="COORDINATES: protein motif:HMM:NF018765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1360 domain-containing protein"
                     /protein_id="HEX5910530.1"
                     /translation="MIENGAPAAPADQNVVQLEAAPEDSYSAGDAKPLAGYATLTTAF
                     AGAVTASEVLRRRRGGPLPKRIGPGDLALLAVATYKLSRLATKARVTSFARAPFTRYT
                     GEAGPSEVSEEPRGTGL"
     gene            complement(353..850)
                     /locus_tag="VFY44_08550"
     CDS             complement(353..850)
                     /locus_tag="VFY44_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910531.1"
                     /translation="MATPTKLESKLAEVLGLAMAAQDAVREVRGMLGGEHQALDKRLV
                     QSRDDAREVERRCKALAGTFKGKKTAILESAKEVKQDTITMRETYLAGEDDPLDGFEF
                     LTMTEAGEVGHWAILAKLNEKAGIEELRKLTEWALPIQERHLQEVLDGSLKLAAAEDP
                     GEEEA"
     gene            complement(856..1809)
                     /locus_tag="VFY44_08555"
     CDS             complement(856..1809)
                     /locus_tag="VFY44_08555"
                     /inference="COORDINATES: protein motif:HMM:NF016925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEX5910532.1"
                     /translation="MILRIDRVQTEMPPPSTPDPAGAAVVQELLGGKFGELSTFMNYT
                     FQSFNFRQRQGARPFFDLIANIAAEEFGHIELVSTAINTMLTGTGEKGELPEPKDLLN
                     RQQIIAGGSATMLQDSNGNAWTGDYVTSTGDLLEDLTHNFFLETGARNNKLKVYEMCD
                     HPAARALTGYLLVRGGVHQIAYARAVENLTGANLMKMFPSPRIPTEKIPESQPHIEKG
                     EHKKLYRFSPSDYLELAAVFNGPHPETGEDLEVVDQAPEGFPPFDLPAQEGVFAPDYA
                     PEEVAEIARNLRQKAGLSDEPTGVVANDTKGVVDKVKDAVS"
     gene            1884..2822
                     /locus_tag="VFY44_08560"
     CDS             1884..2822
                     /locus_tag="VFY44_08560"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX5910533.1"
                     /translation="MTSRSDLVALLREHGLRVTPQRRAILESFGDGPAEHLSADEVHS
                     RAAAVLPEIGRGTVYAALAELTEVGVLSARGVPEPVRYETNTTPHQHFRCRLCLRLFD
                     VDLPEPDAAPLATAGFSVERIVVTAEGVCADCNDFGEGLRAGAERARAKPSGDLPDGV
                     AAGVVDTPLGAVSVGATGQGLVRVVFEDHADVERLEASRRRGSREARQHVADGKAAVA
                     DYFAGRPVRDCVIDFGSLAGAPTLAATMAIPRSQRASYESLDTPAGASERGRTLGANP
                     LAVLIPCHRVTRGSQLPAEYVGGAERRSAMNELERA"
     gene            3084..3818
                     /locus_tag="VFY44_08565"
     CDS             3084..3818
                     /locus_tag="VFY44_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910534.1"
                     /translation="MKLLVGLAGGLLLAMLAVLVAVLASLEGTRSELRTTRVGVTKAE
                     QRSARLGEQVQPLLRAVEPLSAGGAQEELRRTGRAVSGAAGAVPPLAEDVHRGVGIAT
                     FIAQTLHSADLQPALAAVRSLTDTTVPAVDELRPALVALFAELDRPGARSLAECDRRL
                     DARPLSAAGQVGCLLRTVPNVRGLLRDQRDLNARSLATQRSTLAHTRQIDQSFQESLV
                     IQREILVRIRSLDKKTGGTAPPGLAP"
BASE COUNT          495 a         1366 c         1368 g          611 t
ORIGIN      
        1 aggccggtgc cgcgcggctc ctccgagacc tcggagggcc cggcctcgcc ggtgtagcgc
       61 gtgaacgggg cccgcgcgaa gctggtcacc cgcgccttgg tcgccagccg gctgagcttg
      121 tacgtggcca cggccaggag cgcgagatcg cccggcccga tgcgtttcgg caacgggcct
      181 ccgcgccggc gacgaagcac ctccgacgcg gtcacggccc ccgcgaacgc cgtggtcagc
      241 gtcgcgtacc ccgccagcgg cttggcgtct ccggccgagt acgagtcctc cggcgccgct
      301 tccagctgca ccacgttctg gtccgccggc gccgccgggg ccccgttctc gatcaagcct
      361 cctcctcgcc cggatcctcg gcggccgcca gcttgagcga gccgtccagg acctcctgca
      421 ggtggcgctc ctggatcggc agcgcccact cggtgagctt gcgcagctcc tcgatcccgg
      481 ccttctcgtt gagcttggcc aggatcgccc agtggccgac ctcgcccgcc tcggtcatgg
      541 tcaggaactc gaagccgtcg agcgggtcgt cctcgcccgc caggtaggtc tcgcgcatgg
      601 tgatcgtgtc ctgcttgacc tccttggcgc tctccaggat ggccgtcttc ttgcccttga
      661 aggtgccggc cagcgccttg cagcgccgtt cgacctcacg cgcatcgtcg cgggactgca
      721 ccaggcgctt gtccagcgcc tggtgctcgc cgcccagcat gccgcgcacc tcgcgcacgg
      781 catcctgagc ggccatcgcc aggccgagga cctcggccag cttgctctcg agcttggtcg
      841 gagtggccat cgggcttagg agaccgcgtc cttgaccttg tcgacgacac ccttggtgtc
      901 gttggcgacc acgccggtgg gctcgtccga cagcccggcc ttctggcgca ggttgcgggc
      961 gatctcggcg acctcctcgg gggcgtagtc gggcgcgaac acaccctcct gcgcgggcag
     1021 gtcgaacggc gggaagccct cgggggcctg gtcgacgacc tccaggtcct cgccggtctc
     1081 cggatgcggt ccgttgaaca cggccgcgag ctccaggtag tcgctgggcg agaagcggta
     1141 gagcttcttg tgctcgccct tctcgatgtg cggctgcgac tcggggatct tctccgtggg
     1201 gatgcggggc gaggggaaca tcttcatcag gttcgccccg gtcaggttct ccaccgcacg
     1261 cgcgtaggcg atctggtgga cgccgccgcg gacgagcagg tagccggtga gggcgcgtgc
     1321 cgccgggtgg tcgcacatct cgtagacctt gagcttgttg ttgcgggctc cggtctccag
     1381 gaagaagttg tgcgtgaggt cctcgagcag gtcacccgtg gacgtgacgt agtcgccggt
     1441 ccaggcgttg ccgttggagt cctgcagcat cgtggcggac ccgccggcga tgatctgctg
     1501 ccggttgagc aggtccttgg gctccggaag ctcgcccttc tcgcccgttc ccgtgagcat
     1561 ggtgttgatg gccgtcgaga ccagctcgat gtgcccgaac tcctcggcgg cgatgttggc
     1621 gatcaggtcg aagaacggcc gcgcgccctg gcgctggcga aagttgaacg actggaacgt
     1681 gtagttcatg aaggtcgaca gctcgccgaa cttgccgccc agcagctcct gcaccacggc
     1741 ggcgccggcg gggtcggggg tcgagggggg cggcatctcg gtttgcaccc ggtcgatacg
     1801 cagaatcatg taagttctcc tagataggac aaagtcttga ctaagacaac ctgcccggcg
     1861 gcccgtccgg taaacaaggg gccatgacga gccgttccga cctcgtggcg ctgctgcgcg
     1921 agcacgggct gcgggtgacc ccccagcgac gggcgatcct cgaatcgttc ggcgacgggc
     1981 ccgcggagca cctctccgcc gacgaggtcc actcccgcgc cgccgcggtc ctgccggaga
     2041 tcgggcgcgg caccgtgtac gccgcattgg ccgagctgac cgaggtcggt gtgctcagcg
     2101 cgcgcggcgt ccccgagccg gtccggtacg agaccaacac caccccccac cagcacttcc
     2161 gctgccggct ctgcctgcgg ttgttcgacg tcgaccttcc cgagcccgac gccgcaccgc
     2221 tggccactgc ggggttctcg gtcgagcgga tcgtcgtgac ggccgagggc gtctgcgcgg
     2281 actgcaacga cttcggcgag ggattgcgtg ccggggcaga gcgagcacgg gccaagccct
     2341 ccggggacct gcccgatggg gtcgccgcgg gcgtcgtgga caccccgctg ggagccgtct
     2401 ccgtgggcgc caccggccag ggcctggtgc gggtggtgtt cgaggatcac gcggacgtcg
     2461 agcggctcga ggcgtcacgc cgcaggggca gccgcgaggc gcggcagcac gtggcggacg
     2521 gcaaggccgc ggtcgccgac tacttcgcgg gacgccccgt gcgcgactgc gtgatcgact
     2581 tcggcagcct ggcgggcgcg cctacgctgg cggccacgat ggccatcccc cgttcgcagc
     2641 gtgcctcgta cgagagcctg gacacccccg cgggggcatc ggagcgcggc cggaccctgg
     2701 gtgcgaaccc gctcgccgtg ctgatcccgt gccatcgcgt gacccggggc agccagctcc
     2761 cggccgagta cgtgggcggc gccgaacgcc gcagcgcgat gaacgagctc gagcgggcct
     2821 gagcaccacc cggcggtttg ccgcgcccgc gcgcgggtag cccggccggt ccccatgatt
     2881 ctcgatacct cgaacggacg ccggtaacgg tgttccgcat atcccggcgc tcaggcgggc
     2941 ggacgcgggt ctccggcttc cccgggtgcc tgatcacgtc cctggtgctc tccgtcgcgt
     3001 tgacgatcct gctcaatgtg ctgatccggc tgttctaggt gcctgcacag gcagccggga
     3061 cgcctgcccc cgtccctccc gtgatgaagt tgctggtagg cctggccggg gggctgctcc
     3121 tggcgatgct cgcggtgttg gtcgccgtcc tggcgtcgct cgaagggacc aggtcggagc
     3181 tcaggacgac ccgggtcgga gtcaccaagg ccgaacagcg ctctgcccgc ctcggcgagc
     3241 aggtacagcc gctcctgagg gccgtcgagc ccctgtcggc gggaggggcc caggaggagc
     3301 tccgccgtac cggacgggcc gtctccggcg ccgcgggggc cgtgcccccg ctggccgagg
     3361 acgtgcaccg tggggtcggg atcgccacct tcatcgccca gaccctgcac agcgccgacc
     3421 tccagcccgc gctggcggcc gtgcggtcgc tgacggacac gacggtcccg gccgtcgacg
     3481 agctgcggcc ggccctcgtg gccctcttcg ccgagctgga ccggcccggc gcgcgttctc
     3541 tcgccgagtg tgaccggcgg ctcgacgccc ggccgctctc cgccgcgggg caggtgggtt
     3601 gcctcctgcg aaccgttccg aacgtgcgcg ggctgctgcg cgaccagcgg gacctgaacg
     3661 ccaggtcgtt ggccacccag cggtcgacgc tggcccacac ccggcagatc gaccagagct
     3721 tccaggagag cctggtgatc cagcgggaga tcctggtgcg gatccgctcg ctggacaaga
     3781 agacgggcgg aaccgcgccg ccagggttgg cgccctgaga tcggtctcgc tgcgtcggag
//
LOCUS       DASUPW010000381         7292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1891042, whole genome shotgun sequence.
ACCESSION   DASUPW010000381 DASUPW010000000
VERSION     DASUPW010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7292
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1891042"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /gene="tadA"
                     /locus_tag="VFY44_08570"
     CDS             <1..416
                     /gene="tadA"
                     /locus_tag="VFY44_08570"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEX5910535.1"
                     /translation="REAERALEHDDVPVGCVVVAGGEVVAAARNERELRGDPTAHAEI
                     LALREASSRLGSWRLEDTVLYVTLEPCAMCAGAIVLARVPRVVFATEDPKTGAAGSVL
                     NVLAQPALNHRPDVAGGLLAAEAGDMLRAFFAARR"
     gene            432..1556
                     /locus_tag="VFY44_08575"
     CDS             432..1556
                     /locus_tag="VFY44_08575"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5910536.1"
                     /translation="MGDVDFNYSNVPFALSCTFDAVLVLAVVAWAARGERPYPRLAVG
                     GAAVLAVLALKGIVMIRLGLNVSFGVIHVLWLDLVVVLPVAGVAVLCSASLRRHRLLA
                     VAGVAALLLVPVGAYASFIEPSRTVVERAEVPLAPERAGDRPVRIAVVADLQFEHLGG
                     HEREAVATVMAQRPDVILITGDIHQGSREQFARQRRDIRALLSRLRAPGGVYAVHGDT
                     EGPEKARRIYAGTGIDLLINRALPVRVNGRALTVAGIQVNYRSAPAVGTVGALERTPG
                     DRDVRILVTHRPDAVYGLRPRSRVDLTVAGHTHGGQFQLPFAGAPKTASHVPRAVGSG
                     GLHTLSGRRLYVSRGIGMERGQAPSLRLGAPPEVSVLTLR"
     gene            complement(1595..2404)
                     /locus_tag="VFY44_08580"
     CDS             complement(1595..2404)
                     /locus_tag="VFY44_08580"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEX5910537.1"
                     /translation="MSAAAAALGRGVASAEPVAGGDLNDAWRLELDDGTPAFAKTGHP
                     GTYEPEAAGLAWLADGGATVPEVLAASEDVLVLGWVEAARHDPEDLGRMVATMHRAGA
                     GEFGVLADGGHAPLRLGPLEIPSDPAPDWATFYARSRVWPLARRAGLDPDGLGAVERL
                     CERMADFAGPAEPPARLHGDLWSGNVMGGPVLIDPAAYGGHREVDLAMLSLFGSPGDR
                     FLAAYQEVHPLADGHADRVALWQLFPLLVHATLFGGGYGASAVRAARRYIG"
     gene            complement(2401..2865)
                     /locus_tag="VFY44_08585"
     CDS             complement(2401..2865)
                     /locus_tag="VFY44_08585"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEX5910538.1"
                     /translation="MRVLFVCMGNICRSPTAEGVFRALAEEEGVEVEIDSAGTGGWHV
                     GAPPDERATAAAAARGIELGGGARQVRAEDFGEFDLIVAMDAENAAGLQRLAPDGEAA
                     ERIRLMREFDPEADGDLDVPDPYYGGPDGFEDVLDMVERASRGLLEEVRSRR"
     gene            complement(2871..3710)
                     /locus_tag="VFY44_08590"
     CDS             complement(2871..3710)
                     /locus_tag="VFY44_08590"
                     /inference="COORDINATES: protein motif:HMM:NF006133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5910539.1"
                     /translation="MSALTDRTVIMSGGSRGIGLAIAKKAASEGANVAIIAKTDQPHP
                     KLEGTVHTAAAEIEEAGAGGALAIVGDVRDEESVAAAVAQTVERFGGIDIVVNNASAI
                     NLAPMRDLPVKRLDLMLDINLRGTFVLTQAALPHLRESDHAHVLSLSPPLSADHKWLH
                     GHSAYTVAKYGMTMVTLGVAFDEAEAPIAANTLWPRTLIATAAVQNLLGGDEAMRKAR
                     TPEIYADSALAVLKRDPRECTGNSFIDDEVLMEEGVTDFSGYQADPSGELALDLFIEG
                     WPE"
     gene            3776..4534
                     /locus_tag="VFY44_08595"
     CDS             3776..4534
                     /locus_tag="VFY44_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910540.1"
                     /translation="MNRPATLLIAFLLLVAAAPAAGAATFQEGQMKFTVSGSWVATGP
                     TIVDCQDRASGETTVLKPTVTERVTLRSTSAGRVAYKGLVGRSVFFGRIGATPKVELS
                     VSRGPNPPANCSSSPAPDCGSKALRGVANPEFEGPLRMGGKVDLRFDFARSRTSFRFA
                     DPFRRCPEPGAGPGWFAAALGNSGDAQALQAAAIGSPSVKRLYGKRSQTFSGSATKRF
                     KGDPGIGLGPSSATLKMKVKVSRKVTKSQPAFGS"
     gene            4558..4646
                     /locus_tag="VFY44_08600"
     tRNA            4558..4646
                     /locus_tag="VFY44_08600"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4592..4594,aa:Ser,seq:cga)
     gene            4699..5466
                     /locus_tag="VFY44_08605"
     CDS             4699..5466
                     /locus_tag="VFY44_08605"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5910541.1"
                     /translation="MARLARWGAIGTVAAAVVLVPAGLATGGATEPSSGAAARATGCK
                     SKPGLSVVKNGPRTKKRVAITFDDGPSPGTSRLLSVLEAGNVKATFFPMGNKLDRRPG
                     VVKRAVREGHEFQNHSHTHADLGNGGSQATEEMRKATTAIRGATGFTPCVFRPPFDSV
                     GGDLTRRARALGLRTVAADVLPLDYEDPPAGTIVSRVLDATRPGSIIVLHDGQADGDG
                     SRPNTTAAVATIIKRLKARGYRFVTVTKILGYRRTSG"
     gene            5505..6578
                     /locus_tag="VFY44_08610"
     CDS             5505..6578
                     /locus_tag="VFY44_08610"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: protein motif:HMM:TIGR02089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEX5910542.1"
                     /translation="MGEQRRHEIAVIPGDGIGTEVTPAALEVLAAAGRAYGFELVPTE
                     YDWGCDHYAETGAMMPEDALDRLAGADAIFLGAVGRHDVPDHVSLWGLLIPIRRRFQQ
                     YVNERPMRLWPGVRSPLRDVTPEDLDLVVVRENNEGEYSEIGGRLHTGTAIEMAVQET
                     VFTRTGCDRVMDFACRLAGERSGRICSATKSNGIIHTMPYWDERFAAALERHPGLDSE
                     QVLIDALAAMVVLDPGRFDVIVASNLFGDILSDLTAAVAGSIGIAPSANLNPEREHPS
                     MFEPVHGSAPDIAGKGVANPLGAVWSGAQMLEHLGEQEAADAIMAAMEGSLGGGGPRT
                     PDLGGSAATADVVAELASGFASR"
     gene            6597..>7292
                     /locus_tag="VFY44_08615"
     CDS             6597..>7292
                     /locus_tag="VFY44_08615"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX5910543.1"
                     /translation="MGAEEKGPAGTGRTDDAEQMITQTGTYRSLGEQKLSPQEERFER
                     GRRTVGLFLAPAVIVIFLLLPLDLDPSQHKLAAILLGVVTLWITEAVPIPVGGLIGIA
                     LIVLIGVGDADTVLAPFGSSTVFTFIGAFIIAQAMLNHGVARRFAFRMLAVRGVSKST
                     SRTVIAFGLITAMLSAFVSNTATVAMLLPTAIAILGTLANLMQKEGDGSEGFDPLKLR
                     VGVALMLMLAYGAS"
BASE COUNT          925 a         2678 c         2679 g         1010 t
ORIGIN      
        1 tgcgtgaggc ggagcgggcg ctggagcacg acgacgtgcc ggtcggctgc gtggtggtcg
       61 cgggcggtga ggtcgtggcc gcggcccgca acgagcgcga gctgcgtggc gaccccacgg
      121 cccacgccga gatcctggcg ctgcgcgagg cctcgtcccg gctgggctcg tggcggctgg
      181 aggacaccgt gctctacgtg accctggagc cgtgcgccat gtgcgcgggc gcgatcgtgc
      241 tggcccgcgt tccgcgcgtg gtcttcgcca ccgaggaccc caagaccggc gcggccggca
      301 gcgtgctgaa cgtgctggcc cagcccgccc tgaaccaccg gcccgacgtg gccggcgggc
      361 tgctggccgc cgaggccggc gacatgcttc gcgcgttctt cgcggcacgt cgctagctac
      421 cgtccggccg ggtgggggac gtcgacttca actactccaa cgtccccttc gcgctctcgt
      481 gcacgttcga cgccgtgctc gtgctcgccg tggtcgcgtg ggcggctcgg ggtgagcggc
      541 cctacccgcg cctggcggtg ggcggagccg cggtgctggc cgtcctggcg ctgaagggca
      601 tcgtgatgat ccgcctgggg ctgaacgtgt cgttcggcgt gatccacgtc ctgtggctgg
      661 acctcgtcgt ggttctgccc gtggccgggg tggccgtcct gtgcagcgcg tccctgcggc
      721 gccaccgcct gctggcggtg gccggcgtcg ccgccctgtt gctggtgccg gtcggggcct
      781 acgccagctt catcgagccc tcgcgcacgg tcgtcgagcg cgccgaggtg ccgcttgccc
      841 ccgagcgcgc gggcgaccgc ccggtgcgga tcgccgtggt ggcggacctc cagttcgagc
      901 acctgggcgg ccacgagcgc gaggccgtcg cgaccgtgat ggcccagcgc ccggacgtga
      961 tcctgatcac cggcgacatc caccagggct cacgggagca gttcgcccgc cagcgccggg
     1021 acatcagggc tctgctgtcc cggctgcgtg cgcccggcgg cgtctatgcc gtccacggtg
     1081 acaccgaggg cccggagaag gcgcggcgca tctacgccgg gacggggatc gacctgctga
     1141 tcaaccgcgc gctgccggtg cgcgtgaacg gccgtgccct gaccgtggcg ggcatccagg
     1201 tgaactaccg ctcggcaccc gccgtgggca cggtcggcgc gctcgagcgg acacccggtg
     1261 accgggacgt gcggatcctg gtgacccacc gccccgatgc cgtctacggg ctgcgcccgc
     1321 gctcgcgcgt ggacctgacg gtggccggcc acacccacgg gggccagttc cagctcccct
     1381 tcgccggggc gcccaagacg gcctcgcacg tgccccgcgc ggtgggctcc ggcgggctgc
     1441 acacgttgtc ggggcggcgc ctctacgtca gccgcggcat cgggatggag cggggccagg
     1501 cgccgagcct gcgcctgggc gcgccgcccg aggtgtcggt gctcaccctc cgctaggacg
     1561 acctccgcgg cgcccgcgcg ctgcgccgcc tgcgctagcc gatgtagcgg cgcgcggccc
     1621 gcaccgccga ggcgccgtag ccgccgccga acagcgtggc gtgcaccagc agcgggaaca
     1681 gctgccacag ggccacgcgg tcggcgtgcc cgtcggccag cgggtgcacc tcctggtagg
     1741 cggccaggaa gcgatcgccg ggggagccga acagcgacag catcgccagg tcgacctcgc
     1801 ggtggccgcc gtaggcggcg gggtcgatca ggaccgggcc gcccatcacg ttcccgctcc
     1861 acaggtcgcc gtgcagtcgc gccggcggct cggccgggcc ggcgaagtcg gccatccgct
     1921 cgcacaggcg ctcgacggcg ccgagcccgt ccgggtccag cccggcgcgc cgggccagcg
     1981 gccacacccg gctgcgcgcg tagaaggtgg cccagtcggg tgcggggtcg gacgggatct
     2041 ccagcgggcc caggcgcaac ggtgcgtggc cgccgtcggc cagcacgccg aactcgcctg
     2101 cgcccgcgcg gtgcatcgtc gccaccatcc ggcccaggtc ctccgggtcg tggcgggccg
     2161 cctcgaccca gcccagcacc aggacgtcct cgctcgcggc cagcacctcg ggcacggtgg
     2221 cgccgccgtc ggccagccag gccaggccgg ccgcctcggg ctcgtaggtg cccgggtgcc
     2281 cggtcttggc gaacgccggc gtcccgtcgt ccagctccag ccgccaggcg tcgttcaggt
     2341 cgccgccggc caccggctcg gcggaggcga ccccgcggcc cagggccgcg gccgctgcgc
     2401 tcagcgcctg gaccggacct cctccagcag cccccggctg gcgcgctcga ccatgtccag
     2461 gacgtcctcg aacccgtccg ggccgccgta gtaggggtcg ggcacgtcca ggtcgccgtc
     2521 cgcctcgggg tcgaactcgc gcatcaggcg gatccgctcg gcggcctcgc cgtcgggggc
     2581 cagtcgctgc agccccgccg cgttctcggc gtccatggcc acgatcaggt cgaactcgcc
     2641 gaagtcctcg gcgcgcacct ggcgcgcacc gccgccgagc tcgatcccgc gcgccgcggc
     2701 ggccgcggtc gcgcgctcgt ccggcggggc gcccacgtgc cagccgccgg tgccggcgct
     2761 gtcgatctcg acctcgacgc cctcctcctc ggccagcgcg cggaacacgc cctcggccgt
     2821 cggcgagcgg cagatgttgc ccatgcagac gaagaggacc cgcacggcgg ctactcgggc
     2881 cagccctcga tgaacaggtc cagcgccagc tcgcccgacg ggtcggcctg gtagccggag
     2941 aagtcggtca cgccctcctc catcagcacc tcgtcgtcga tgaacgagtt gccggtgcac
     3001 tcgcgcgggt cacgcttgag caccgccagc gcggagtcgg cgtagatctc cggggtgcgc
     3061 gccttgcgca tggcctcgtc gccgcccagc aggttctgca ccgccgccgt ggcgatcagc
     3121 gtccgcggcc agagcgtgtt ggccgcgatc ggggcctcgg cttcgtcgaa ggccacgccc
     3181 agggtgacca tcgtcatccc gtacttggcc accgtgtagg ccgagtggcc gtggagccac
     3241 ttgtggtcgg ccgacagcgg cggcgacagg ctcagcacgt gcgcgtggtc ggactcgcgc
     3301 aggtgcggca gcgcggcctg ggtgagcacg aacgtgccgc gcaggttgat gtccagcatc
     3361 aggtccaggc gcttgaccgg caggtcgcgc atgggggcca ggttgatcgc gctggcgttg
     3421 ttgaccacga tgtcgatccc gccgaagcgc tccaccgtct gggccaccgc cgcggccacc
     3481 gactcctcgt cgcgcacgtc gccgacgatg gccagcgcgc caccggcgcc ggcctcctcg
     3541 atctccgcgg cggccgtgtg caccgtgccc tcgagcttgg ggtggggctg gtcggtcttg
     3601 gcgatgatgg ccacgttcgc cccctccgac gcggccttct tggcgatggc caggccgatt
     3661 ccgcggctgc cgccggacat gatcacggtg cgatcggtga gtgcgctcat ggccgggcac
     3721 cctaatcgtc ctcaggtagg acgcgcgcac gcccggccac atgcatcctg ggcacatgaa
     3781 tcgccccgcc accctcctca tcgccttcct cctcctggtg gccgccgcgc ccgccgccgg
     3841 ggccgcgacc ttccaggagg gccagatgaa gttcaccgtc agcggctcct gggtggccac
     3901 cggccccacc atcgtcgact gccaggaccg cgccagcggc gagacgaccg tgctcaagcc
     3961 caccgtgacc gagcgggtca cgttaaggag cacgtcggcc gggcgggtgg cctacaaggg
     4021 cctggtcggc cggtcggtct tcttcgggcg catcggcgcc acgccgaagg tcgagctgtc
     4081 ggtctcgcgc gggcccaacc cgcccgcgaa ctgctcgtcc agcccggcgc ccgattgtgg
     4141 ttccaaggcg ctgagaggcg tggccaaccc cgagttcgag gggccgctgc gcatgggcgg
     4201 caaggtcgac ctgcgcttcg acttcgccag gagcaggacg tcgttccgct tcgccgaccc
     4261 gttccgccgg tgcccggagc ccggcgccgg cccgggctgg ttcgccgccg ctctgggcaa
     4321 cagcggggac gcccaggcgc tccaggccgc ggcgatcggc agcccgtcgg tcaagcgcct
     4381 ctacggcaag cgcagccaga cgttctccgg gtcggcgacg aagcggttca agggcgatcc
     4441 cggcatcggc ctcggcccgt ccagcgcgac gctgaagatg aaggtcaagg tcagccgcaa
     4501 ggtcacgaag agccaaccgg ccttcggaag ctaggctttc cggcgccgct tgaccctgga
     4561 ggggtgccgg agcggttgaa cggggcggtc tcgaaaaccg ttgtgcgtcg taaggcgtac
     4621 cgtgggttcg aatcccaccc cctccgctga agtcccaggc cgttgccgtc ccggtggacc
     4681 gcctgtacgg tggcctcgat ggctcggctg gcgaggtggg gtgcgatcgg gacggtggct
     4741 gcggccgtgg tcctggtccc ggccggcctg gccacgggcg gcgcgaccga gccctcgtcc
     4801 ggcgccgccg cgcgcgcgac cggctgcaag agcaagcccg gcctttccgt ggtcaagaac
     4861 ggcccgcgca cgaagaagcg cgtcgcgatc accttcgacg acggcccctc gccggggacg
     4921 agccggctgc tctccgtcct cgaggccggc aacgtcaagg cgaccttctt cccgatgggc
     4981 aacaagctcg accgccggcc cggcgtggtc aagcgcgccg tccgcgaggg ccacgagttc
     5041 cagaaccact cccacaccca cgccgacctc ggcaacggcg gttcgcaggc gaccgaggag
     5101 atgcggaagg cgacgaccgc gatccgcggc gcgacggggt tcacgccgtg cgtcttccgg
     5161 ccgccgttcg actcggtcgg cggcgacctg acgcggcgcg cccgtgcgct ggggctcagg
     5221 acggtcgcgg cggacgtcct gccgctggac tacgaggacc cgccggccgg cacgatcgtc
     5281 tcgcgtgtgc tggacgccac ccgcccgggg tcgatcatcg tgctgcacga cggccaggcc
     5341 gacggtgacg ggtcgcgccc caacacgacc gcggcggtgg ccacgatcat caagcggctg
     5401 aaggcccgcg gctaccggtt cgtgaccgtc acgaagatcc tcggctaccg gcggacgtcc
     5461 gggtaggccg gttacgaacg ccgcccacgg gttacgagac tcccgtgggc gaacagcgac
     5521 gacatgagat agcggtcatc cccggcgacg ggatcgggac ggaggtgacc ccggccgcgc
     5581 tggaggtgct ggcggcggcg gggcgcgcgt acgggttcga gctcgtgccc accgagtacg
     5641 actggggctg cgaccactac gcggagaccg gggcgatgat gcccgaggac gcgctcgacc
     5701 ggctggcggg cgccgacgcg atcttcctgg gcgcggtcgg ccggcacgac gtgcccgacc
     5761 acgtgtcgct ctggggcctg ctgatcccga tccggcgccg cttccagcag tacgtgaacg
     5821 agcgcccgat gcggctgtgg cccggcgtgc ggtcgccgct gcgcgacgtc acgcccgagg
     5881 acctggacct ggtggtggtg cgcgagaaca acgagggcga gtactcggag atcggggggc
     5941 ggctgcacac ggggaccgcg atcgagatgg cggtccagga gaccgtgttc acgcgcacgg
     6001 gctgcgaccg cgtcatggac ttcgcctgcc gcctggccgg cgagcgctcg gggcggatct
     6061 gctcggcgac gaagtccaac gggatcatcc acacgatgcc gtactgggac gagcgcttcg
     6121 cggccgcgct ggagcgccac ccggggctgg actccgagca ggtgctgatc gacgcgctgg
     6181 cggccatggt ggtgctggac ccgggccggt tcgacgtgat cgtggcctcc aacctgttcg
     6241 gcgacatcct cagcgacctg acggccgcgg tcgcgggctc gatcgggatc gccccctcgg
     6301 ccaacctcaa ccccgagcgc gagcacccgt cgatgttcga gccggtgcac ggctcggcgc
     6361 ccgacatcgc cggcaagggc gtggccaacc cgctgggcgc ggtctggtcc ggcgcccaga
     6421 tgctcgagca cctcggcgag caggaggccg ccgacgcgat catggcggcc atggagggct
     6481 cgctcggcgg cggcggtccg cgcacgccgg acctgggcgg gtcggccgcc accgcggacg
     6541 tggtggccga gctggcttcg ggttttgcct cgcgctgacg agggcaagcg cgccccatgg
     6601 gagcggagga gaagggcccc gcggggacgg gaaggaccga cgacgccgag cagatgatca
     6661 cccagaccgg tacctaccgg agcctgggcg agcagaagct gagcccgcag gaggagcgct
     6721 tcgagcgcgg ccgccgcacg gtgggcctgt tcctggcccc ggcggtgatc gtgatcttcc
     6781 tgctgctgcc gctggacctg gacccgagcc agcacaagct cgcggcgatc ctgctgggcg
     6841 tcgtgacgct gtggatcacc gaggccgtgc cgatccccgt cggcggcctg atcggcatcg
     6901 cgctgatcgt cctgatcggc gtgggcgacg ccgacaccgt gctggcgccg ttcggctcct
     6961 ccacggtctt caccttcatc ggcgcgttca tcatcgccca ggccatgctc aaccacggcg
     7021 tggcgcggcg gttcgcgttc cggatgctcg ccgtccgcgg ggtgtccaag agcacctcgc
     7081 gcaccgtgat cgccttcggc ctgatcaccg cgatgctgtc ggcgttcgtg tccaacaccg
     7141 cgacggtggc gatgctgctg cccaccgcga tcgccatcct gggcacgctg gccaacctga
     7201 tgcagaagga gggggacggc tcggagggct tcgacccgct gaagctgcgc gtgggcgtcg
     7261 cgctgatgct gatgctggcc tacggcgcca gc
//
LOCUS       DASUPW010000382         2303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1059781, whole genome shotgun sequence.
ACCESSION   DASUPW010000382 DASUPW010000000
VERSION     DASUPW010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059781"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VFY44_08620"
     CDS             complement(<1..189)
                     /locus_tag="VFY44_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910544.1"
                     /translation="MQSSRLVRAVFALLVLATLGTFFATQRRKTEGPVVLRFAGDPRH
                     ISPNNDGVRDSTRVGFDLS"
     gene            231..1322
                     /locus_tag="VFY44_08625"
     CDS             231..1322
                     /locus_tag="VFY44_08625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5910545.1"
                     /translation="MRVQIVDPPAYTPPYDHSLAAALARAGADVELLTSRYPYGAAPD
                     QGGYAVREGFYRRATAGDRGTAARRALRLAEHLPGMFAARDASAFDVVHYQWLTLEPV
                     DALLLARGAPRVFTAHNVLRRGTRSRTALRRALARMDAVVAHTEAGAADLRAETDVPV
                     HVIPHGAFDYLAHQEHEQPLPAELAGVEGPVILCFGILRPYKGVDVLVDAFREIEGAE
                     LWIAGRPWMDTGPLREAAARAGGTVRFIDRFLPDAQLPALFRRADVVTLPYRRIDQSG
                     VLYTALAFGKAMVLSDVGGFGEVGRVDGAAELVPPGDPAALREALAGLLADPARRAGL
                     EERARAAARGRYSWDAIGRRTLELYEGLA"
     gene            1322..2107
                     /locus_tag="VFY44_08630"
     CDS             1322..2107
                     /locus_tag="VFY44_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEX5910546.1"
                     /translation="MRAILMGKHKRSAVSALEHLLRRGVQVVAVVAPPDPVGVADTQR
                     LDRAAQLNGIEVTTDDELYARIERDELADVDLVLSFLFWKRLRAPLIELGRIGCLNFH
                     PAPLPDMRGMGGFNVAILEGFAEWGVSGHFVDADFDTGDLVRVDRFPIEPDATALSLD
                     MDSQVRLLALFKDVVDMAVDGADLPRTPQGEGRYVTKAEFEAMRRVPPGEDAETLERR
                     IRAFWYPPYDGATIEIAGKAVSLVDRALLDEIAAANREAGRFP"
     gene            complement(2108..>2303)
                     /locus_tag="VFY44_08635"
     CDS             complement(2108..>2303)
                     /locus_tag="VFY44_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910547.1"
                     /translation="GTVTNFNDQQRAESVVLYAPGAEREAAEVGRRLKISQREPIDAE
                     SQGVAGDATVAVVTGQDKTQ"
BASE COUNT          291 a          833 c          868 g          311 t
ORIGIN      
        1 ggacaggtcg aagcccaccc gcgtcgagtc ccggacgccg tcgttgttgg gcgagatgtg
       61 ccgcggatcg ccggcgaagc gcagcaccac cgggccctcg gtcttccgcc gctgggtggc
      121 gaagaaggtg cccagcgtgg ccagcacgag cagggcgaag acggcacgga ccaggcggct
      181 cgactgcacc ggcgcaacgc tattggaccc cggcggccag aatgcccccg gtgcgcgtgc
      241 aaatcgtcga cccgccggcc tacacgccgc cctacgacca ttcgctggcc gcggcgctgg
      301 cccgcgcggg cgccgacgtg gagctgctga ccagcagata tccctacggc gcggcccccg
      361 accagggtgg ctacgcggtg cgcgagggct tctaccgccg tgccacggcg ggcgaccgcg
      421 gcaccgcggc ccggcgcgcg ctacggctgg ccgagcacct cccgggcatg ttcgccgcac
      481 gcgacgcgtc ggcgttcgac gtcgtgcact accagtggct gacgctcgag ccggtggacg
      541 cgctgctgct ggcgcgtggc gccccgcgcg tgttcaccgc gcacaacgtg ctccgccgcg
      601 ggacccggtc gcggacagcg ctgcgacggg ccctggcgcg gatggacgcc gtggtcgcgc
      661 acaccgaggc cggggccgcc gacctgcggg ccgagaccga cgtgccggtc cacgtgatcc
      721 cccacggcgc gttcgactac ctggcccacc aggagcacga gcagccgctg cccgccgagc
      781 tggcgggcgt cgagggcccg gtgatcctgt gcttcgggat cctgcgcccg tacaagggcg
      841 tcgacgtgct ggtggacgcc ttccgggaga tcgagggagc cgagctgtgg atcgccggcc
      901 gcccgtggat ggacaccggc ccgctgcgcg aggccgcggc ccgggccggg ggcaccgtgc
      961 gcttcatcga ccggttcctg ccggacgcgc agctgcccgc gctgttccgc cgcgccgacg
     1021 tggtcacgct cccctaccgg cggatcgacc agtccggggt cctgtacacg gcgctcgcgt
     1081 tcggcaaggc gatggtgctc tcggacgtgg gcggcttcgg cgaggtcggg cgggtggacg
     1141 gcgcggccga gctggtgccc ccgggggacc cggccgcgct gcgcgaggcc ctggccgggc
     1201 tgctcgcgga cccggcccgg cgggccgggc tcgaggagcg cgcgcgggcc gccgcccggg
     1261 gccgctactc ctgggacgcc atcgggcgtc gtacgcttga gctctacgag gggctggcct
     1321 gatgcgagcg atcctgatgg gaaagcacaa gcgctccgcc gtgagcgcgc tggagcacct
     1381 gctgcgccgc ggcgtacagg tggtggccgt ggtggcgccg cccgaccccg tgggcgtggc
     1441 ggacacgcag cgcctggacc gcgcggccca gctgaacggc atcgaggtga ccaccgacga
     1501 cgagctctac gcccggatcg agcgcgatga gctggccgac gtcgacctgg tgctgtcgtt
     1561 cctgttctgg aagcgcctgc gcgcgcccct gatcgagctg ggacggatcg gctgcctgaa
     1621 cttccacccc gcccccctgc ccgacatgcg cggcatgggc ggcttcaacg tcgcgatcct
     1681 ggaggggttc gccgagtggg gtgtgtcggg ccacttcgtc gacgccgact tcgacaccgg
     1741 cgacctggtg cgcgtcgacc gcttcccgat cgagccggac gccaccgcgc tgtcgctgga
     1801 catggacagc caggtgcgcc tgctggcgct gttcaaggac gtggtggaca tggccgtgga
     1861 cggcgccgac ctgccgcgca cgccccaggg cgagggccgc tacgtgacca aggccgagtt
     1921 cgaggccatg cgcagggttc cgcccggcga ggacgccgag accctggagc ggcgcatacg
     1981 cgccttctgg tacccgccct acgacggggc gacgatcgag atcgccggga aggcggtcag
     2041 cctggtcgac cgggcgctgc tggacgagat cgcggcggcg aacagggaag caggacgctt
     2101 cccgtagcta ttgcgtcttg tcctggccgg tgacgaccgc gacggtggcg tctcccgcca
     2161 ctccctggct ctcggcgtcg atgggctcgc gctgggagat cttcagccgc cggcccacct
     2221 cggcggcctc gcgctcggcg ccgggggcgt agagcaccac cgactcggcg cgctgctggt
     2281 cgttgaagtt ggtgaccgtg ccc
//
LOCUS       DASUPW010000383         2882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1059894, whole genome shotgun sequence.
ACCESSION   DASUPW010000383 DASUPW010000000
VERSION     DASUPW010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059894"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..318
                     /locus_tag="VFY44_08640"
     CDS             145..318
                     /locus_tag="VFY44_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910548.1"
                     /translation="MPGNSRRLRIVVDWTVGLLFGRASAQLGNLGHPTSLAEYSEDNP
                     RGGERPGEPQGVD"
     gene            315..1271
                     /locus_tag="VFY44_08645"
     CDS             315..1271
                     /locus_tag="VFY44_08645"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5910549.1"
                     /translation="MNAVPAVDGLTFRPGRAADLRPTFELSRLSLHGMAESLGVDSGP
                     APEGEELDREWARRRPLVEFAFAQEGTTVIGEMGGELVGFARTCRFGNMEELTELHVR
                     PSHKGRGIGPALLGQCWPGDPTPQMGRVGVAPGVSRVLNAVTAFGMMPAAGHWHMRAR
                     TSDYVEARSRITDAAEPPVVVLEPDRARAEWQALEPGAIGHDRPGLHEFFARTRTCLA
                     SVDDDGRPSALCWAGMDDAVGPAVGRTSQDLVPVVLQALDRVAKTQEPETLSVYCATH
                     SWWLLRRLRSLGFQIVWPIWVMSSVPLPGLDRYLPTRPAHVL"
     gene            1297..1467
                     /locus_tag="VFY44_08650"
     CDS             1297..1467
                     /locus_tag="VFY44_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910550.1"
                     /translation="MADAASAVGRKALAVLILVAAAYLLFKVAIGFVMGIVWVLVAVV
                     AVIGVVWALRVL"
     gene            1472..1750
                     /locus_tag="VFY44_08655"
     CDS             1472..1750
                     /locus_tag="VFY44_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910551.1"
                     /translation="MSALVLVIFFGLSTGTIGKIKGSSFFIWFLIGFCLPVIGTIVAI
                     LYRNERAEPLRRCEECGKVVAVHQQVCRRCGNDLYFPEPEPQQRLFEA"
     gene            1839..>2882
                     /locus_tag="VFY44_08660"
     CDS             1839..>2882
                     /locus_tag="VFY44_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5910552.1"
                     /translation="MRAVDAIMECLKREGVEHVFGLPGGANLPTYDALVDAGLRHIQC
                     RHEAGAGHAAEGYAKATGKVGVALATSGPGATNLVTPIADAMMDSVPTVFITGQVRTD
                     LLGTDGFQEADISGITMPIVKHSIMIQDPQQIPEAIHEAFHIARTGRPGPVLVDVPQD
                     LSRADIPYEPVTTVDLPGYQPTVEGNTKQIRLAAKALANANRPVIYAGGGVINADAAA
                     ELVELCTSDRFPVTCTLMGLGAFPAPHEQWLGMLGMHGTRSANYAMDEADLICCIGAR
                     FDDRITGKLSEFAPRAKFIHIDVDPAEISKNVPAHIPIVGDAKNIIPKLNAEYRALDT
                     DSSRLDGWWQRIKG"
BASE COUNT          411 a         1054 c         1005 g          412 t
ORIGIN      
        1 accagctcgc cgcccatctc accgatgacc gtcgtgccct cctgggcgaa ggcgaactcg
       61 accagcggcc gccggcgggc ccactcgcgg tccagctcct cgccctcggg cgcggggccg
      121 ctgtcgacgc ccagcgactc cgccatgccc gggaactcgc ggcggctgcg gatcgtggtc
      181 gactggaccg tgggcctgct gttcggccgc gcgtcggcgc agctgggcaa cctgggacat
      241 cccacctcgc tggccgagta cagcgaggac aacccgcgcg ggggcgagcg gcctggcgaa
      301 ccgcaaggcg tggattgaac gccgtccccg cagtcgacgg tctgaccttc cggccgggtc
      361 gcgccgccga cctccggccc acgttcgagc tctcgcggct ctccctgcac ggcatggcgg
      421 agtcgctggg cgtggacagc ggccccgcgc ccgagggcga ggagctggac cgcgagtggg
      481 cccgccggcg gccgctggtc gagttcgcct tcgcccagga gggcacgacg gtcatcggtg
      541 agatgggcgg cgagctggtg ggcttcgccc gcacctgccg gttcgggaac atggaggagc
      601 tgaccgagct gcacgtgcgg ccgagccaca aggggcgggg catcgggccg gcgctgctgg
      661 gccagtgctg gcccggcgac ccgaccccgc agatgggtcg cgtgggggtg gcgccgggcg
      721 tctcgcgcgt gctgaacgcc gtgaccgcgt tcggcatgat gcccgcggcc gggcactggc
      781 acatgcgcgc ccgcacgtcc gactacgtgg aggcgcggtc gcgcatcacc gacgcggcgg
      841 agccgccggt ggtggtcctg gagcccgacc gcgcacgcgc cgagtggcag gcgctggagc
      901 ccggcgccat cggccacgac cgccccggcc tgcacgagtt cttcgcccgc acgcgcacct
      961 gcctggcctc ggtcgacgac gacggccggc cctccgcgct gtgctgggcc ggcatggacg
     1021 acgccgtcgg gcccgccgtg ggccggacct cccaggacct ggtgcccgtg gtgctccagg
     1081 cgctggaccg ggtggccaag acccaggagc cggagacgct gagcgtgtac tgcgccaccc
     1141 acagctggtg gctgctgcgg cggctgcgga gcctgggctt ccagatcgtg tggccgatct
     1201 gggtcatgag ctcggtgccg ctgcccgggc tcgaccgcta cctgcccacg cgcccggccc
     1261 acgtgctcta gctcggcccc tccctacaat cggtgtatgg ccgatgccgc atcagcagtg
     1321 ggtcgcaagg cgctcgcggt cctgatcctg gtcgccgccg cctacctgct cttcaaggtg
     1381 gcgatcggct tcgtgatggg catcgtgtgg gtgctcgtcg ccgtcgtggc cgtgatcggc
     1441 gtcgtctggg cgctacgggt gctctgagcg cgtgtccgcg ctcgtgctgg tcatcttctt
     1501 cgggctctcc acggggacca tcgggaagat caagggctcg tcgttcttca tctggttcct
     1561 gatcggcttc tgcctgccgg tcatcgggac gatcgtcgcc atcctctacc gcaacgagcg
     1621 ggccgagcca ctacgccggt gcgaggagtg cggcaaggtc gtcgccgtcc accagcaggt
     1681 gtgccggcgc tgcggaaacg acctctactt tcccgagccg gagccccagc agcgcctctt
     1741 tgaggcctga cagacggctg gtaggatcag cccttcggcc ccggcgcaca ccacgtcgcg
     1801 ggacacagtg caaaccggaa gatcccggaa ggagccctat gcgagcagtc gacgccatca
     1861 tggaatgcct caagcgcgag ggcgtcgagc acgtattcgg gctcccgggc ggtgccaacc
     1921 tccccaccta cgacgcgctg gtcgatgccg gcctgcgtca catccagtgc cgccacgagg
     1981 cgggcgccgg acacgccgcc gagggctacg ccaaggccac cggcaaggtc ggcgtggcgc
     2041 tggccacctc ggggccgggc gcgaccaacc tggtcacgcc gatcgccgac gcgatgatgg
     2101 acagcgtgcc gacggtgttc atcaccggcc aggtgcgcac cgacctgctg ggcaccgacg
     2161 gcttccagga ggccgacatc tcgggcatca cgatgcccat cgtcaagcac tcgatcatga
     2221 tccaggatcc ccagcagatc ccggaggcca tccacgaggc gttccacatc gcccgcaccg
     2281 gccgccccgg tcccgtgctg gtcgacgtgc cccaggacct ctcgcgcgcc gacatcccgt
     2341 acgagccggt caccaccgtc gacctgccgg gctaccagcc caccgtggag ggcaacacga
     2401 agcagatccg cctggcggcc aaggcgctgg ccaacgccaa ccggccggtc atctacgccg
     2461 gcggcggcgt gatcaacgcc gacgcggcgg ccgagctggt cgagctgtgc acgtccgacc
     2521 ggttcccggt cacctgcacg ctcatggggc tgggcgcgtt cccggcgccg cacgagcagt
     2581 ggctgggcat gctgggcatg cacggcaccc gctcggccaa ctacgccatg gacgaggccg
     2641 acctgatctg ctgcatcggg gcacggttcg acgaccgcat cacgggcaag ctgtcggagt
     2701 tcgcgccgcg ggccaagttc atccacatcg acgtcgaccc cgccgagatc tccaagaacg
     2761 tgccggccca catcccgatc gtgggcgacg ccaagaacat catccccaag ctcaacgccg
     2821 agtaccgggc gctggacacc gactcgtcgc ggctcgacgg ctggtggcag cggatcaagg
     2881 gc
//
LOCUS       DASUPW010000384         3161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_229507, whole genome shotgun sequence.
ACCESSION   DASUPW010000384 DASUPW010000000
VERSION     DASUPW010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229507"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..127
                     /locus_tag="VFY44_08665"
     tRNA            55..127
                     /locus_tag="VFY44_08665"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Glu,seq:ttc)
     gene            178..1638
                     /locus_tag="VFY44_08670"
     CDS             178..1638
                     /locus_tag="VFY44_08670"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEX5910553.1"
                     /translation="MAPAVPTFASGWSFADTEPVADRDELTGLGSRRHLLGELEQSCE
                     QARRGGRPAALLFVDLNDFKLVNDSLGHQVGDQLLKEVAKRFKGAVRGDELLARVGGD
                     EFALLVRDLLPHGEAVDPPRAHELAEAIGRRLIDSLAAPFDLDGIELLVDACVGISLM
                     PGDADSSETLRRHADAAMYEAKATGASVVVYAAGTPDPLERLEVAARLRHAIDRGELE
                     LHYQPIVSLALRGTVGMEALVRWRDPLRGGGLVPPDEFIPIAEGTGVIEPLGEWVLDE
                     LCRQAAEWSAEGMHPHLGYNVSPRQLHRPGFVERLAERVRRHELDPARLILELTESSW
                     SLDASRLLPTLQQLDQAGFPLAIDDFGAGYSSLWRLRELPVKVIKIDRELLHGVPGER
                     QATAVVAAIMALARACDCDVVAEGVETAEQREFLADQGCLLAQGYHFARPVPAEEATR
                     MLRDDLVVSRREAPAMDEPQAVRVVSASASMSPSGS"
     gene            complement(1584..1937)
                     /locus_tag="VFY44_08675"
     CDS             complement(1584..1937)
                     /locus_tag="VFY44_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910554.1"
                     /translation="MDRGIFFRSLAVQALLVGALFAALALALPKSFFEDYGWVTGPVA
                     WIGCSILTARILALPAGLVLFAALAGGVAGFLVSLAASHAAGLVVSLLVFAASCGGYE
                     PEGDIDADADTTRTA"
     gene            complement(1948..2709)
                     /locus_tag="VFY44_08680"
     CDS             complement(1948..2709)
                     /locus_tag="VFY44_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5910555.1"
                     /translation="MDSEDQETYGKSADPAGADVTFLRGAVPAPEPLPRGQHDLSPEY
                     VARHQRSRIQEATTFVLAERGYGAATIGDIVARAHVSRRTFYAHFDSKEHCVLATFGA
                     ASECIADAVRTAYTQEAEWDAAVAAGLGQLMHVLVEYPQTARTCFLEIRSVGAMAAEP
                     IHAAHMMCIDALRSAVNDRAGAPAVGDLALEMGMGGLVTVIRARLTAGDPAALQRELP
                     EIAAALLAPLVGDDATRAVVERLDAAPGVRAAHAI"
     gene            2766..2838
                     /locus_tag="VFY44_08685"
     tRNA            2766..2838
                     /locus_tag="VFY44_08685"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2799..2801,aa:Val,seq:tac)
     gene            complement(2865..>3161)
                     /locus_tag="VFY44_08690"
     CDS             complement(2865..>3161)
                     /locus_tag="VFY44_08690"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5910556.1"
                     /translation="AAIAERLMGATAAEWGHTLQAAGVPAGQVNNIAGALELAAQLGL
                     APTVTLPGPGGADVTLPANPVGMSATPPRYDAGPPALGAMSAEEALALVRGRSG"
BASE COUNT          411 a         1159 c         1187 g          404 t
ORIGIN      
        1 gagctacggg ggcccgctca tgctagagcg gccccggcct agactggcgt gcgcgccccc
       61 atggtctagc ggtcaggacg ccgccctttc aaggcggaga tcgcgggttc gaatcccgct
      121 gggggtatcc catccggggc actcaagtgc catctcgggg gtgccgatca ggtacaggtg
      181 gctcccgccg tccccacctt cgcctccggg tggtcgttcg ccgacaccga gccggtcgcc
      241 gaccgggacg agctgacggg cctgggcagc cggcgccacc tgctcgggga gctggagcag
      301 agctgcgagc aggctaggcg cggcggccgc cccgcggcgc tcctgttcgt ggacctgaac
      361 gacttcaagc tggtcaacga cagcctcggg caccaggtgg gagaccagct gctgaaggag
      421 gtcgcgaagc gcttcaaggg cgcggtgcgc ggggacgagc tgctggcgcg cgtcggcggc
      481 gacgagttcg ccctcctggt ccgggacctg ctgccgcacg gcgaggccgt cgacccgccc
      541 agggcgcacg agctggccga ggccatcggc cgccggctga tcgattcgct ggccgccccg
      601 ttcgacctgg acggcatcga gttgctggtg gacgcgtgcg tgggcatcag cctgatgccc
      661 ggcgacgccg actccagcga gacgctgcgc cgccacgccg atgccgccat gtacgaggcc
      721 aaggccacgg gtgccagcgt ggtggtctac gcggcgggga cccccgaccc gctcgagcgg
      781 ctcgaggtgg ccgcgcgcct gcgccacgcc atcgaccgcg gcgagctgga gctgcactac
      841 cagccgatcg tcagcctggc tctgcgcggc accgtgggca tggaggcgct ggtgcgctgg
      901 cgcgacccgc tgcgcggcgg cgggctggtg ccgccggacg agttcatccc catcgccgag
      961 gggaccggcg tgatcgagcc cctgggcgag tgggtcctgg acgagctctg ccgccaggcg
     1021 gccgagtggt cggccgaggg catgcacccg cacctgggct acaacgtctc gccgcgccag
     1081 ctgcaccggc ccgggttcgt ggagcggctc gcggagcgcg tgcgccggca cgagctcgac
     1141 ccggcacgcc tgatcctgga gctgaccgag tcgtcgtggt cgctggacgc gtcacggctg
     1201 ctgcccaccc tccagcaact cgaccaggcg ggcttcccgc tggccatcga cgacttcggc
     1261 gcgggctact cgtcgctgtg gcgcctgcgc gagctgccgg tcaaggtgat caagatcgac
     1321 cgcgagctgc tgcacggcgt gcccggcgag cgccaggcca ccgcggtggt cgcggccatc
     1381 atggcgctgg cccgggcctg cgactgcgac gtggtggccg agggcgtgga gacggccgag
     1441 cagcgcgagt tcctggccga ccagggctgc ctgctggcgc agggctacca cttcgcccgc
     1501 cccgtccccg ccgaggaggc cacgcgcatg ctgcgcgacg acctggtggt ctcgcggcgc
     1561 gaggcgccgg ccatggacga gcctcaggcc gtgcgcgtgg tgtcggcgtc cgcgtcgatg
     1621 tcgccctcgg gctcgtagcc cccgcaggac gccgcgaaca ccagcaggct gaccaccagg
     1681 cccgccgcgt ggctggcggc caggctgacc aggaagcccg ccaccccacc ggccagcgcg
     1741 gcgaacagca cgaggcctgc gggaagggcc aggatgcggg cggtcaggat cgagcagccg
     1801 atccacgcca ccggccccgt gacccagccg tagtcctcga agaagctctt gggcagcgcc
     1861 agcgcgagcg ccgcgaacag ggccccgacc aacagggcct ggaccgccag ggagcggaag
     1921 aagatgccgc gatccacggc ggccagtcta gatggcgtgc gcggcgcgga cgcccggtgc
     1981 tgcgtccagg cgctccacca cggcccgggt ggcgtcgtcg ccgaccagcg gggccaggag
     2041 cgccgccgcg atctccggca gctcccgctg cagggccgcc ggatcgccgg cggtgagccg
     2101 ggcccggatg acggtgacca gcccgcccat gcccatctcc agcgccaggt cgccgaccgc
     2161 gggcgcgccg gcccggtcgt tcaccgcgct ccgcaacgcg tcgatgcaca tcatgtgcgc
     2221 ggcgtggatg ggctccgcgg ccatcgctcc caccgagcgg atctccagga agcaggtgcg
     2281 cgccgtctgc gggtactcca ccagcacgtg catcagctgg ccgagcccgg cggccaccgc
     2341 cgcgtcccac tcggcctcct gtgtgtaggc cgtccgcacg gcgtcggcga tgcactccga
     2401 cgcggcgccg aaggtggcca ggacgcagtg ctccttggag tcgaagtggg cgtagaaggt
     2461 gcgtcgcgac acgtgcgcgc gggccacgat gtcgccgatc gtggcggcgc cgtagccgcg
     2521 ctcggccagg acgaacgtgg tggcctcctg gatccgtgac cgctggtggc gggccacgta
     2581 ctccggcgac aggtcgtgct ggccccgggg cagcggctcc ggtgcgggca cggctccccg
     2641 gaggaaggtc acgtcggcgc cggcggggtc ggcgctcttg ccgtacgtct cctggtcctc
     2701 ggaatccatt ccggcgacca tcgtagggca gcttcggcga ccgggctgcg aagatgaccg
     2761 gtcgcgggcg cgtagctcag ctggtagagc atcgccctta caaggcgagg gtccctggtt
     2821 cgagcccagg cgcgcccatc ccgcgccgga ccaggcccga tcggtcagcc cgagcgcccc
     2881 cgcaccaggg ccagcgcctc ctcggcgctc atggcgccca gcgccggcgg gccggcgtcg
     2941 tagcgcggcg gcgtggccga catgccgacc gggttcgcgg gcagcgtcac gtcggcccct
     3001 ccggggcccg ggagcgtgac ggtgggggcc aggcccagct gcgcggccag ctcgagggcg
     3061 ccggcgatgt tgttgacctg gcccgccggc accccggccg cctggagcgt gtgcccccat
     3121 tcggcggcgg tggcgcccat cagccgctcg gcgatcgcgg c
//
LOCUS       DASUPW010000385         3117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_229650, whole genome shotgun sequence.
ACCESSION   DASUPW010000385 DASUPW010000000
VERSION     DASUPW010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229650"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VFY44_08695"
     CDS             <1..265
                     /locus_tag="VFY44_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910557.1"
                     /translation="SANSVEVYGQTRPGGSATVAIQSQAAGDQFGTVRTIRTNSQGMF
                     RVNISKKSAFRLKWRLSGISNATGATITSREAKAGRKLSFYRN"
     gene            complement(262..1089)
                     /locus_tag="VFY44_08700"
     CDS             complement(262..1089)
                     /locus_tag="VFY44_08700"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5910558.1"
                     /translation="MPDRRPETISHRLAGSPLARVAALPVRLRTVLRHDARVLGASAR
                     WLVRSREHTNYTFELTPLNRVHLAWWVANVVGAPVERIRGYMDEADGDTALEDHVRRA
                     TARSDRRRLADPDVHLHRRLGWYALVRELQPECVVETGTDKGLGSVVLAAALLRNGHG
                     RLITMDINPTAGYLIGGPYADVTELRVGDSVAGLAAMEEQVDFFLHDSWHSRDHELAE
                     YQAVAPRLSGGAVVLSDNSHVMDALPAWAELTGRRFHYFAEEPRDHWYPGAGIGLAR"
     gene            1144..2724
                     /locus_tag="VFY44_08705"
     CDS             1144..2724
                     /locus_tag="VFY44_08705"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX5910559.1"
                     /translation="MPDPDANGLISSYHPRDGFFDEAVDADGRPHPYAEALVESLDRL
                     GADGLAEAADRRDAIFVQQGITFEASGEDGPVKDRPFPLDLIPRIMPASEWRIIKRGV
                     AQRVRALNRFVEDVYHERGIVREGIVPWELVVTRPNFARAAHRIRPPGGVWCHVSGCD
                     LVRDVDGSWKVLEDNVRVPSGISYVLENRIAMSRLVPDLFDKYSVRPVNDYPSLLLDA
                     LRAVAPGGGEQPTVVVWTPGSANSAYFEHAFLARQMGVELVEASDLVVRDTTVFMRTT
                     RGLSRVDAIYRRIDDDFIDPLEFRPDSLLGVPGLMRAYRAGNVAIANALGTGVADDKA
                     IYHYVPEMIRFYLGEEPILDNVKTYLLGDREQREHALSRLDELVVKPTGESGGKGVFI
                     GPHATEEQIAEQRRLLSVAPERWIAQETVNLSTVPTVRPDGSLAPCHVDLRPFAVFGD
                     EISIVPGGLTRVALREGSMIVNSSQGGGSKDTWVLADDLEEPPPGDTFDPFDPPTMPG
                     LRQAGWHQQERQQQQEDD"
     gene            2724..>3117
                     /locus_tag="VFY44_08710"
     CDS             2724..>3117
                     /locus_tag="VFY44_08710"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEX5910560.1"
                     /translation="MLARNAHDVYWLGRHLVRAEHTARMLDGLFHADLQGRQDDPTSV
                     TLTWDSLLTIMGSQGEAGDGPAQRNQVIQALTLDPHNPVSIRACVERAREGARTVRDT
                     ISAEMWESVNTFNLGMQGANLDSGLRTGP"
BASE COUNT          458 a         1138 c         1097 g          424 t
ORIGIN      
        1 ctcggccaac tcggtcgagg tctacgggca gacgcgcccg ggcggctcgg ccaccgtggc
       61 catccagtcg caggccgccg gcgaccagtt cggcaccgtg cgcacgatca ggaccaactc
      121 gcagggcatg ttccgcgtga acatctccaa gaagagcgcg ttccgcctga agtggcgcct
      181 gagcggcatc agcaacgcga ccggtgcgac catcaccagc cgcgaggcca aggccggcag
      241 gaagctcagc ttctacagga actagcgcgc gaggccgatg ccggcgcccg ggtaccagtg
      301 gtcccggggc tcctcggcga agtagtggaa gcggcgccct gtcagctcgg cccaggccgg
      361 cagggcgtcc atcacatgtg agttgtcgct gagcaccacg gcgccgccgg acagtcgcgg
      421 ggcgaccgcc tggtactcgg ccagctcgtg gtcgcggctg tgccagctgt cgtgcaggaa
      481 gaagtcgacc tgctcctcca tcgcggccag cccggccacg gagtccccca cccgcagctc
      541 cgtgacgtcc gcgtagggcc cgccgatcag gtacccggcg gtcgggttga tgtccatggt
      601 gatcaggcgc ccgtggccgt tgcgcagcag cgcggcggcc agcaccaccg agccgaggcc
      661 cttgtcggtg ccggtctcga ccacgcactc gggctggagc tccctgacca gcgcgtacca
      721 gcccagcctg cggtgcaggt gcacgtccgg gtcggccagc cgccgccggt cactgcgggc
      781 cgtggcccgg cggacgtggt cctcgagcgc ggtgtccccg tcggcctcgt ccatgtagcc
      841 gcggatccgc tcgacgggcg caccgaccac gttggccacc caccacgcca ggtgcacgcg
      901 gttcagcggg gtgagctcga acgtgtagtt ggtgtgctcg cgcgagcgca ccagccagcg
      961 cgcggaggcc ccgagcacgc gcgcgtcgtg gcggagcacg gtccgcagcc gcaccgggag
     1021 ggccgcgacc cgggccagcg ggctgccggc caggcgatgg ctgatcgtct ccggtcggcg
     1081 gtcgggcatc ggcgggccat gctgtcagac atccggggga gaactggata gaactacgcg
     1141 ttcgtgcccg accctgatgc caatggcctg atcagctcct accacccccg tgacggcttc
     1201 ttcgacgagg ccgtggacgc ggacgggcgc ccccacccct acgcggaggc cctggtggag
     1261 tcgctggacc ggctcggggc cgacgggctg gccgaggcgg ccgaccgccg cgacgcgatc
     1321 ttcgtgcagc agggcatcac cttcgaggcc agcggcgagg acggcccggt caaggaccgg
     1381 ccgttcccgc tggacctgat cccgcggatc atgcccgcgt ccgagtggcg gatcatcaag
     1441 cgcggcgtgg cccagcgcgt ccgggcgctc aaccgcttcg tggaggacgt ctaccacgag
     1501 cgcggcatcg tccgcgaggg catcgtgccc tgggagctcg tggtcacgcg gcccaacttc
     1561 gcccgcgccg cccaccgcat caggccgccc ggcggcgtgt ggtgccacgt gtccggctgc
     1621 gacctggtgc gcgacgtgga cggctcctgg aaggtgctgg aggacaacgt gcgcgtcccg
     1681 tcgggcatct cgtacgtgct cgagaaccgg atcgccatga gccggctggt ccctgacctg
     1741 ttcgacaagt actcggtgcg gccggtcaac gactacccgt cgctgctgct ggacgccctg
     1801 cgcgcggtgg ccccgggggg cggcgagcag cccaccgtcg tggtgtggac gcccgggtcg
     1861 gccaacagcg cctacttcga gcacgccttc ctggcccgcc agatgggcgt cgagctggtc
     1921 gaggcctccg acctggtcgt gcgcgacacc acggtgttca tgcgcaccac gcgcgggctc
     1981 tcgcgcgtgg acgccatcta ccgccgcatc gacgacgact tcatcgaccc gctggagttc
     2041 cggccggact cgctgctggg cgtgcccggc ctgatgcgcg cctaccgcgc cggcaacgtg
     2101 gccatcgcca acgcgctggg caccggggtg gcagacgaca aggcgatcta ccactacgtg
     2161 ccggagatga tccgcttcta cctgggcgag gagccgatcc tggacaacgt caagacctac
     2221 ctcctgggcg accgcgagca gcgcgagcac gcgctgtcgc ggctggacga gctggtggtc
     2281 aagcccaccg gcgagtccgg cggcaagggc gtgttcatcg gcccgcacgc caccgaggag
     2341 cagatcgcag agcagcgccg gctcctgtcg gtcgcgcccg agcgctggat cgcgcaggag
     2401 acggtgaacc tgtccacggt gcccacggtc cgccccgacg gctcgctggc cccgtgccac
     2461 gtggacctgc gcccgttcgc cgtgttcggc gacgagatct cgatcgtccc cggcggcctg
     2521 acgcgggtgg cgctgcgcga gggatcgatg atcgtgaaca gctcgcaggg cggcggctcg
     2581 aaggacacct gggtgctggc cgacgacctg gaggagccgc caccgggcga taccttcgac
     2641 ccgttcgacc cgcccaccat gcccggcctg cgccaggccg gctggcacca gcaggagcgc
     2701 caacagcagc aggaggacga ctgatgctgg cccgcaacgc ccacgacgtc tattggctcg
     2761 gccgccacct ggtccgggcc gagcacacgg cgcgcatgct cgacgggctg ttccacgccg
     2821 acctccaggg ccgccaggac gaccccacct cggtcacgct gacgtgggac tccctgctca
     2881 cgatcatggg ctcccagggc gaggccggcg acggccccgc ccagcgcaac caggtgatcc
     2941 aggcgctcac gctcgatccg cacaacccgg tgtccatccg ggcctgcgtt gaacgcgccc
     3001 gcgagggcgc ccgcacggtg cgcgacacca tctcggccga gatgtgggag tcggtcaaca
     3061 ccttcaacct gggcatgcag ggagccaacc tggactccgg cctgcgcacg gggccgt
//
LOCUS       DASUPW010000386         2001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_644434, whole genome shotgun sequence.
ACCESSION   DASUPW010000386 DASUPW010000000
VERSION     DASUPW010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644434"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="VFY44_08715"
     CDS             <1..881
                     /locus_tag="VFY44_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX5910561.1"
                     /translation="YGRGRPGPALKVALLVDSSGAGYGVDRVEETPGSVLAAGLSEQA
                     HRRVYLRSFARVGALSSDLSGQVEQCLPIEPDVAVILIGVNDVTHTVLPQTSVRHLSE
                     AVRRLRDADVEVLVGTCPDLGTVRPIAPPLRQVARIWSRRLAAAQAITVVEAGGRTVS
                     LGSILGPEFDAAPALLFGPDRFHPSAEGYAQLVAVLLPSTLAAVGLAREQEIQPETFR
                     GEGVLPIARAAVEAARTPGTELDGTEIGGSRRGVRGLWVELRHRRRRPRTEGEAPETA
                     EHTGLPEDTGTGRSRK"
     gene            complement(934..1800)
                     /locus_tag="VFY44_08720"
     CDS             complement(934..1800)
                     /locus_tag="VFY44_08720"
                     /inference="COORDINATES: protein motif:HMM:NF024221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEX5910562.1"
                     /translation="MRSNNPVFNRTPEFNGQSTNAYGNQTYPGNGASYGGYGQQGQSD
                     PSTWGTGTPGSPTQVDQGRMTVDSVVQKTAISLAVVVLAAGVTWFMSPPVDGTEDLGP
                     LYLAVMVGSFGAFGLSLVNSFKRVISPALVLAFCALEGVALGALSKVFDARFGDGIVT
                     QAVLGTFAAFAGTLAAYKFFNIQVGNKFRTFVIAAMFGMVGLGLLEMVLGAFGNQLGL
                     FGFGGAGLLFAVLGLVLGVFMLILDFDFVEQGVAAGLPERESWRAAFALTVSLVWIYT
                     NLLRILAIFSQD"
BASE COUNT          293 a          719 c          727 g          262 t
ORIGIN      
        1 ggtacggccg cggccgcccg gggccggcgc tcaaggtggc gctgctcgtc gactccagcg
       61 gcgccgggta cggcgtcgac cgggtcgagg agacccccgg ctcggtgctc gccgccggcc
      121 tgtccgagca ggcacaccgc cgggtctacc tgcggtcgtt cgccagggtg ggggcgctgt
      181 ccagcgacct gagcggccag gtggagcagt gcctgccgat cgagccggac gtcgccgtca
      241 tcctgatcgg cgtcaacgac gtcacccaca cggtcctccc ccagacctcc gtgcggcacc
      301 tgtccgaggc ggtacgccga ctccgggacg ccgacgtcga ggtgctggtc ggcacctgcc
      361 ctgacctggg cacggtccgc ccgatcgcgc cgcccctgcg ccaggtcgcg cggatctggt
      421 cgcgtcggct ggccgccgcg caggcgatca ccgtcgtcga ggccgggggc cgcacggtct
      481 cgctgggctc gatcctcggc cccgagttcg atgcggcacc agccctgctg ttcgggccgg
      541 accgcttcca cccctcggcc gagggctacg cgcagctcgt cgccgtgctg ctcccgtcga
      601 cgctggccgc ggtcggcctg gcccgcgagc aggagatcca gcccgagacg tttcgcggcg
      661 agggcgtgct gccgatcgcg cgggccgcgg tcgaggccgc ccgcaccccc ggcaccgagc
      721 tcgacggcac cgagatcggc ggctcgcgcc gcggcgtacg cgggctctgg gtggagctgc
      781 ggcaccggcg ccggcgcccg cgcaccgagg gcgaggcccc ggagaccgcc gagcacaccg
      841 ggctgccgga ggacaccggc accgggcgca gccggaaata gcgagcggcc cgagctccgg
      901 agagctcgag ccgctcgatc gatcagctgc tggtcagtcc tggctgaaga tcgccaggat
      961 gcgcagcagg ttggtgtaga tccagaccag gctgacggtc agcgcgaacg cggcccgcca
     1021 ggactcccgc tccgggaggc cggcggcgac gccctgctcc acgaagtcga agtccaggat
     1081 cagcatgaac acgcccagca ccaggccgag gacggcgaac agcaggcccg caccgccgaa
     1141 cccgaacagg cccagctggt tgccgaacgc gccgagcacc atctcgagca ggccgaggcc
     1201 gaccatgccg aacatcgccg cgatcacgaa cgtgcggaac ttgttgccga cctggatgtt
     1261 gaagaacttg tacgccgcca gggtgccggc gaacgcggcg aacgtgccga gcacggcctg
     1321 ggtgacgatg ccgtccccga accgcgcgtc gaacaccttg ctgagcgcgc cgagtgcgac
     1381 accctcgagg gcgcagaacg ccagcaccag ggccgggctg atcacgcgct tgaacgagtt
     1441 gaccagggac aggccgaagg cgccgaacga gccgaccatc acggccaggt agagcggccc
     1501 gaggtcctcc gtgccgtcga ccgggggcga catgaaccac gtgacacccg cggcgagaac
     1561 caccacggcg agcgagatcg cggtcttctg gaccaccgag tccacggtca tccggccctg
     1621 gtcgacctgc gtgggcgagc cgggcgtgcc ggtgccccag gtggaggggt cggactggcc
     1681 ctgctggccg tagccgccgt aggacgcgcc attgccgggg taggtctggt tgccgtaggc
     1741 gttcgtcgac tgcccgttga actcgggcgt ccggttgaac accgggttgt tgctcctcat
     1801 tggtctcctc cttggaggcc gggtggcggc cgtcgctctg acgactcacc tgtgcggccc
     1861 agtctagagg ccgtactcat ccgacaaacc gcacgacggg gggatgttgt tccggagcgc
     1921 gcggctcccg ggggtggcag gtcgttccga tggcgcgccg ttcggcgcgg gggcagaccc
     1981 gcgagcgggg ttcctctaca g
//
LOCUS       DASUPW010000387         3081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_921738, whole genome shotgun sequence.
ACCESSION   DASUPW010000387 DASUPW010000000
VERSION     DASUPW010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921738"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="VFY44_08725"
     CDS             complement(<1..1177)
                     /locus_tag="VFY44_08725"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX5910563.1"
                     /translation="MLLAEQLVGEARARRQQRLDRRERVVEGASAALYLAVALSLLAF
                     VPSTREADPLLMGALVVGYVAVSRVRFEYSDVYVSAEQLVFIPMLVLVPIEWVPLLVA
                     AAGLLSVLPEVMSGTWHHQRVLYAFSDSWFAIGPVLVILALAPELPSLDDWGVYLAAF
                     VAQVVFDLGAGLIRDSLQTRPPLIEFLRSSLGTVQVDAILAPIAFVLALEARADPLVL
                     LAIGPLVWLLRVFSKDRQERYSTTLELNRAYRGTVMLLADVVESDDGYTGEHSRSVVE
                     LVQAVMDHLHVDPATRQEMEFAALLHDVGKISIPKDILNKPAKLTAEEFELMKTHTVE
                     GQFLLDRVGGLLGRIGEVVRSCHERWDGKGYPDGLKGEQIPLAARIVFVCDAYNAMTT
                     "
     gene            1361..>3081
                     /gene="alaS"
                     /locus_tag="VFY44_08730"
     CDS             1361..>3081
                     /gene="alaS"
                     /locus_tag="VFY44_08730"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX5910564.1"
                     /translation="MQSAEIREKYLSFFEGRDHKRMPSASLVPASFDPTVLLTTAGMQ
                     PFKPYFRGEETPPSPRLTSCQKVFRTTDIENVGLTARHLTFFEMLGNFSVGDYFKKGA
                     AEFAWELSTEGFGFDPERIWITVFGGDDELGLGPDEEAIEVWRAIGVPDERIVRLGTE
                     DNFWQSGPTGPCGPCSELYLDRGVDFGGADDLPGDDTERFLEYWNLVFMQFELHADGS
                     LTDLPQQNIDTGLGLDRMAAIMQDVPSVYETDLFAPLIEFGEQRSGRTSGQDFTTTRA
                     LRILADHGRGATFLLADGVVPSNEDRGYILRRILRRAIQQGRRLGIADGFLPDLCQVV
                     VDKMGEPYPQLHEERDTIMRWAAAEEEGFGRTLAQGERLLDELVAKARRDGAQAIPAE
                     EAFRLHDTFGFPFELTTELLAEQGLGVDQEGFAGLMDQARVVARGRGRAGAGDDVRER
                     AAGFAKNTGFTTNFVGYESTEAETVLRAVQRDNGTILAKLEDSPFYAEGGGQVSDGGL
                     VETPSGRARVSAVLRLGSDQALALEPLEGDLVEGEPVHAVVERDARLATMRNHTATHL
                     LHAALRER"
BASE COUNT          494 a         1051 c         1112 g          424 t
ORIGIN      
        1 cggtggtcat ggcgttgtag gcgtcgcaga cgaagacgat ccgggcggcc agcgggatct
       61 gctcgccctt caggccgtcc gggtagccct tcccgtccca gcgctcgtga caggagcgca
      121 cgacctcgcc gatgcggccc agcaggccgc ccacgcgatc cagaaggaac tggccctcga
      181 ccgtgtgcgt cttcatgagc tcgaactcct cggcggtcag cttggccggc ttgttcagga
      241 tgtccttggg gatcgagatc ttgcccacgt cgtgcagcag cgcggcgaac tccatctcct
      301 gccgtgtggc cgggtccacg tgcaggtggt ccatcaccgc ctgcaccagc tcgacgaccg
      361 agcgcgagtg ctcgccggtg tagccgtcgt cggactcgac gacgtcggcc agcagcatca
      421 ccgtgccgcg gtaggcgcgg ttgagctcga gggtcgtgga gtagcgctcc tgccggtcct
      481 tggagaacac gcgcagcagc cacaccagcg gcccgatggc cagcagcacc agcggatcgg
      541 cgcgcgcctc gagcgccagc acgaacgcga tcggcgccag gatggcgtcc acctggacgg
      601 tgcccaggga cgagcgcagg aactcgatca ggggtgggcg ggtctggagg ctgtcgcgga
      661 tcagccctgc gccgaggtcg aagacgacct gtgcgacgaa ggccgccagg tagacccccc
      721 agtcgtccag cgacgggagc tcgggggcca gcgccaggat gaccagcacg ggaccgatgg
      781 cgaaccagga gtcgctgaag gcgtagagca cgcgctggtg gtgccacgtg ccggacatga
      841 cctccggcag gaccgagagc aggccggccg cggccacgag cagggggacc cactcgatcg
      901 ggaccagcac gagcatgggg atgaagacca gctgctcggc ggagacgtac acgtcggagt
      961 actcgaaccg cacccgggag accgcgacgt agcccaccac cagggcgccc atgagcaggg
     1021 ggtcggcctc gcgcgtggaa gggacgaagg ccagcagcga cagggcgacg gccaggtaca
     1081 acgcggccga tgcgccctcg accacgcgct cgcgccgatc caggcgctgt tggcgccgag
     1141 cacgcgcttc tccgaccagt tgctcggcaa gcagcattac ttgctcatgt tcggctgctt
     1201 gctacgcgca cattcgatcg caaagcaaag tgcgcaggtt cagggggtca accctgcagt
     1261 gcgggcacac ctttccggcg aaggatcgtg atatgcaggc atgtttccag ccgggaccgg
     1321 cgcagaccgg cgaccgcggt cgactcttag actgcatgcg gtgcagtcgg cggagatacg
     1381 agagaagtac ctgtccttct tcgaggggcg cgatcacaag cggatgccgt ccgcgtcgct
     1441 ggtccccgcc agcttcgatc cgaccgtcct gctgaccacc gccggcatgc agccgttcaa
     1501 gccctacttc cggggtgagg agacgccccc gtcgccgcgg ctgacctcgt gccagaaggt
     1561 gttccggacc accgacatcg agaacgtcgg cctgaccgcc aggcacctga cgttcttcga
     1621 gatgctgggc aacttctcgg ttggggacta cttcaagaag ggcgccgccg agttcgcctg
     1681 ggagctctcg acggagggct tcggcttcga ccccgagcgg atctggatca cggtcttcgg
     1741 cggcgacgac gagctggggc tcgggcccga cgaggaggcg atcgaggtct ggcgggccat
     1801 cggggtgccc gacgagcgca tcgtgcgcct gggcaccgag gacaacttct ggcagtccgg
     1861 gccgacgggc ccgtgcgggc cgtgctcgga gctctacctg gaccggggcg tggacttcgg
     1921 gggcgcggac gatctccccg gcgacgacac cgagcgcttc ctggagtact ggaacctggt
     1981 gttcatgcag ttcgagctgc acgccgacgg ctcgctcacc gacctcccgc agcagaacat
     2041 cgacacaggc ctgggcctgg accggatggc ggcgatcatg caggacgtgc cctcggtcta
     2101 cgagaccgac ctgttcgcgc cgctgatcga gttcggcgag cagcgctccg gtcgcacgtc
     2161 gggccaggac ttcaccacca cgcgtgccct gcgcatcctg gccgaccacg ggcgcggggc
     2221 cacgttcctg ctggccgacg gggtggtgcc ctcgaacgag gaccgcgggt acatcctgcg
     2281 ccggatcctg cgccgcgcga tccagcaggg gcggcggctg gggatcgccg acggcttcct
     2341 cccggacctg tgccaggtgg tcgtcgacaa gatgggcgag ccctacccgc agctgcacga
     2401 ggagcgcgac acgatcatgc gctgggccgc tgccgaggag gagggcttcg gccgcaccct
     2461 ggcccagggc gagcggctcc tggacgagct ggtcgccaag gcgcgtcgcg acggggccca
     2521 ggccatcccc gccgaggagg cgttccggct gcacgacacc ttcggcttcc cgttcgagct
     2581 gaccaccgag ctgctggccg agcagggcct gggcgtggac caggagggct tcgccggcct
     2641 gatggaccag gcgcgggtgg tcgcgcgcgg gcggggccgc gcgggggcgg gcgacgacgt
     2701 gcgcgagcgg gccgccggct tcgccaagaa cacgggcttc accaccaact tcgtgggcta
     2761 cgagtccacc gaggccgaaa cggtgctgcg cgccgtgcag cgcgacaacg ggaccatcct
     2821 ggccaagctc gaggacagcc cgttctacgc cgagggcggg ggacaggtct ccgacggcgg
     2881 cctggtggag acgcccagcg gccgcgcccg cgtctccgcg gtgctgcgcc tgggcagcga
     2941 ccaggccctg gcgctggagc cgctggaggg cgacctggtg gagggcgagc cggtgcacgc
     3001 cgtcgtcgag cgcgacgcgc gcctggccac gatgcgcaac cacacggcca cccacctgct
     3061 gcacgccgcg ctgcgcgagc g
//
LOCUS       DASUPW010000388         3183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_783426, whole genome shotgun sequence.
ACCESSION   DASUPW010000388 DASUPW010000000
VERSION     DASUPW010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783426"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="VFY44_08735"
     CDS             complement(<1..1273)
                     /locus_tag="VFY44_08735"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5910565.1"
                     /translation="MATAQASSTKWAGSLEPSPAGPIEEAEAATLSKPPAVDLPRALQ
                     VLRFSQRQIEFVFACRRRLGPVFRVKGVIPGDPTVTSHPDHVKSLFTAKPGLAPSLTG
                     ESPLRPILGSGSVLTSVGDRHMRQRKLLLPPFHGEAVARYVEMIDEVTARELDSWPAG
                     EPMALAPRMQAITLDVIMRGIFGVDDPQKGTPEYGLREATRRLTELSTKPIAQIGELT
                     NLGRAEPVGLTKLAMKVIDRPTFQVIADRRRATDLDERTDIMSLLMRARTEDGEALTD
                     QELRDELLTLVLAGHETTANQLAWTWERLVRWPDALERLTEAVRTDAEDADAVVEQTI
                     HESMRSRPVIPIIGRRVQVPWRLGEYAVEAGDPVSMSILLIHHREDIYPDPFSFRPER
                     WEDVKPDTYRWIPFGGGVRRCLGAALAMAEQR"
     gene            complement(1283..2245)
                     /gene="nudC"
                     /locus_tag="VFY44_08740"
     CDS             complement(1283..2245)
                     /gene="nudC"
                     /locus_tag="VFY44_08740"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEX5910566.1"
                     /translation="MPDPNTFTGALIDRAADGRRTDASWVHEQMSHPGARAVLAADEG
                     MRVRDGHLALVPLSESPAPVDDGVPLLLGLDDDGPVFAIDEGPATEPGRRPAFVGAGG
                     RRGEPPPTDADHLGLREAAQTLDQAESGLAAYAAAILNWHRSHRFCANCAAPTDVAEA
                     GMVRSCPNCRAQHHPRVDPVVIMVVTDGAERVLLGRQKVWPARRYSALAGFVSPGESL
                     EEAVLREVFEEVGVHVGAPQYRSSQPWPFPGSLMLGFTCPWQEGEIGGADQELEDARW
                     FTRDQVAEAAADDSWDTPDDGGDGLLLPPRSAIARRLIEGWLAG"
     gene            2284..>3183
                     /locus_tag="VFY44_08745"
     CDS             2284..>3183
                     /locus_tag="VFY44_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5910567.1"
                     /translation="MLAAYASSPNADDPLSALEVGDRPDPEPGDGWVTVDVKAASLNM
                     HDVFTLRGVGIKEEQFPMILGCDGAGTLEDGSEVVIHGVVPSPGWVGDETLDPKRTLL
                     SEKHQGTIAQKVVVPQRNVLPKPDSLSFAEAACLGTAWLTAYRMLFVKSGLRPGQTML
                     VQGASGGVATALTALGSNAGIRVWVTGSTEEKRAIAEAQGADATFETGARLPERVDAV
                     FETVGGATWSHSLKSLKPGGTVVIAGATLGDADKGELQRLFFLQLNVVGSTMGTRDEL
                     ADLLAYLEREEITPRIGAEVALAD"
BASE COUNT          413 a         1155 c         1190 g          425 t
ORIGIN      
        1 cgcgctgctc ggccatggcc agcgccgcgc ccaggcagcg gcggacgccg ccgccgaagg
       61 ggatccagcg gtaggtgtcg ggcttgacgt cctcccagcg ctcgggccgg aacgagaacg
      121 gatcggggta gatgtcctcg cggtggtgga tcagcaggat gctcatcgac accgggtcgc
      181 cggcctccac cgcgtactcg cccaggcgcc agggcacctg cacgcggcgg ccgatgatgg
      241 ggatcaccgg gcgtgagcgc atgctctcgt ggatcgtctg ctcgaccacc gcgtcggcgt
      301 cctcggcgtc ggtgcggacg gcctcggtca ggcgctcgag cgcgtcgggc cagcgcacca
      361 ggcgctccca cgtccaggcc agctggttgg ccgtggtctc gtgcccggcc agcaccagcg
      421 tcaacagctc gtcgcgcagc tcctggtcgg tcagcgcctc gccgtcctcg gtgcgggcgc
      481 gcatcagcag ggacatgatg tccgtgcgct cgtccaggtc ggtggcgcgg cggcggtcgg
      541 cgatcacctg gaaggtcggc ctgtcgatga ccttcatcgc cagcttggtc aggcccaccg
      601 gctcggcgcg gccgaggttg gtcagctcgc cgatctgcgc gatcggcttg gtcgacagct
      661 cggtcaggcg gcgggtggcc tcgcgcagcc cgtactcggg cgtgcccttc tgcgggtcgt
      721 ccacgccgaa gatgccgcgc atgatcacgt cgagcgtgat ggcctgcatc cggggcgcca
      781 gcgccatcgg ctcgccggcc ggccacgagt ccagctcgcg cgcggtgacc tcgtcgatca
      841 tctccacgta gcgcgccacg gcctcgccgt ggaacggcgg cagcagcagc ttgcgctggc
      901 gcatgtggcg gtcgcccacc gaggtcagca ccgagcccga gcccaggatc ggccgcagcg
      961 gggactcgcc cgtgagcgag ggagccagcc ccggcttggc cgtgaacagc gacttcacgt
     1021 ggtcggggtg gctggtcacc gtcgggtcgc ccgggatcac gcccttgacg cggaacaccg
     1081 ggccgagccg ccgccggcag gcgaacacga actcgatctg gcgctggctg aagcgaagca
     1141 cctggagcgc ccgcggcagg tcgacggcgg gcggcttgga cagcgtggcg gcctcggcct
     1201 cctcgatcgg tccggcgggc gagggctcga ggctgccggc ccacttcgtg ctggatgcct
     1261 gggcggtggc catcacgaca gactaaccgg ccagccagcc ctcgatcagg cggcgcgcga
     1321 tcgccgaccg gggcggcagc agcaggccgt cgccgccgtc gtcgggcgtg tcccacgagt
     1381 cgtcggcggc cgcctcggcc acctggtcgc gcgtgaacca gcgcgcgtcc tccagctcct
     1441 ggtccgcgcc gccgatctcg ccctcctgcc acgggcaggt gaagcccagc atcagcgagc
     1501 ccgggaacgg ccacggctgc gagctgcggt actgcggcgc gcccacgtgc accccgacct
     1561 cctcgaacac ctcgcgcagc accgcctcct ccaggctctc gccgggcgag acgaaccccg
     1621 ccagcgccga gtagcgccgg gccggccaca ccttctggcg ccccagcagc acgcgctcgg
     1681 cgccgtcggt cacgaccatg atcaccaccg ggtccacgcg cgggtggtgc tgggcgcggc
     1741 agttggggca gctgcgcacc atgcccgcct cggccacgtc ggtgggcgcg gcgcagttgg
     1801 cacagaagcg gtggctgcgg tgccagttca ggatcgcggc ggcgtaggcg gccaggccgc
     1861 tctcggcctg gtccagggtc tgggccgcct cgcgcagacc caggtggtcg gcgtcggtgg
     1921 gcggcggctc gccgcggcgc ccgccggcgc ccacgaacgc gggccggcgg ccgggctcgg
     1981 tggcggggcc ctcgtcgatg gcgaacacgg ggccgtcgtc gtccaggccc agcagcagcg
     2041 gcaccccgtc gtccacgggc gcgggcgact cgctcagcgg gaccagcgcc aggtgcccgt
     2101 cgcggacgcg catgccctcg tcggccgcca gcacggcgcg ggcgccgggg tggctcatct
     2161 gctcatggac ccaggaggcg tcggtgcggc ggccgtcggc cgcgcggtcg atcagggcgc
     2221 cggtgaaggt gttggggtcg ggcacgggca tgaatctagt ccggtcccgg gtaggggttc
     2281 cccatgctcg ccgcctacgc ctcatccccc aacgccgacg atcccctctc cgccctggag
     2341 gtcggcgacc gccccgaccc cgagcccggc gacggctggg tgacggtgga cgtcaaggcc
     2401 gcgagcctga acatgcacga cgtgttcacc ctgcgcggcg tggggatcaa ggaggagcag
     2461 tttccgatga tcctgggctg cgacggcgcc gggacgctgg aggacggcag cgaggtggtc
     2521 atccacggcg tcgtgccctc gcccggctgg gtcggcgacg agacgctcga ccccaagcgc
     2581 acgctgctca gcgagaagca ccagggcacg atcgcccaga aggtggtggt gccacagcgc
     2641 aacgtgctgc ccaagcccga ctccctgtcc ttcgccgagg ccgcctgcct gggcacggcg
     2701 tggctgaccg cctaccggat gctgttcgtg aagtccgggc tgcggccggg ccagacgatg
     2761 ctggtgcagg gcgcctccgg cggcgtggcc accgcgctga cggcgctggg ctcgaacgcg
     2821 ggcatccgcg tgtgggtcac cgggtcgacc gaggagaagc gggcgatcgc cgaggcccag
     2881 ggcgccgatg ccaccttcga gaccggcgcg cggctgcccg agcgcgtgga cgccgtgttc
     2941 gagaccgtgg gcggcgccac ctggtcgcac tcgctgaagt cgctgaagcc gggcggcacg
     3001 gtggtcatcg ccggcgccac gttgggcgac gccgacaagg gcgagctcca gcggctgttc
     3061 ttcctccagc tgaacgtggt cggctccacg atgggcacgc gcgacgagct ggccgacctg
     3121 ctggcctacc tggagcgcga ggagatcacg ccccgcatcg gcgcagaggt ggccctggcc
     3181 gat
//
LOCUS       DASUPW010000389         2043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_922157, whole genome shotgun sequence.
ACCESSION   DASUPW010000389 DASUPW010000000
VERSION     DASUPW010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922157"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..>2043)
                     /locus_tag="VFY44_08750"
     CDS             complement(61..>2043)
                     /locus_tag="VFY44_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5910568.1"
                     /translation="GYVDPELLALALADGARRQGVAVHTGVTVTGIGVDGGRVTGVQT
                     SAGPVAAEVVVIAAGAATGVVGAMAGAVIPITPMRHQYVVTEPLAEDVEGIATVRDPD
                     HIMYFRPHDSGGLLVGGYSREPVTWDTASPLAEPRTTFDPDMERFAESWEGARNRVPA
                     LRSLEPAKVVNAPEAFTPDGDFILGETEVEGLWTAAGFCVHGLAGAGGVGKVMAEWIT
                     DGRPEFDMASMDIRRFGAHYGSRSFSRTRALSAYSRYYDIVYPHQEFDAGRPLRLSPA
                     YPRLAALDAEFGEKAGWERVNWFGSNAGAGDESLRPRGWAGEFWSPAIGAECLAARDT
                     AALFDQSSFAKIDVRGPGAAAALSRLCANDVAGDVGRAVYTQLLNDGGGIEADLTVTR
                     VGEESFRVVTGTAFGRHDLAWIRRHLPRDGSVTAEDVTGSRCCYCLWGPAAREILSSL
                     TDDDLAFKFLRAREISVGPVPVFAQRITFVGELGWELYASAEFGLTLWDLLWQAGREH
                     GLVGGGYRAIDSMRLEKGYRVWGLDITPETTPDEAGLSFAVAMDKEGGFIGRDALAAA
                     RAGDGPAQRLRCLVLDDPRSVCLGSEPVRVDGSPVGRVTSGGYGHRVSRSIAFAYLPS
                     EVEEGARVDVAVFGEWVGAEVVAEPLYDPKFERVRG"
BASE COUNT          282 a          812 c          715 g          234 t
ORIGIN      
        1 gttcgccgtc gaggtcgcgg cggacacctg cactcgtccg ggcgccgacc ctccccgacg
       61 ctagccgcgc acgcgctcga acttcgggtc gtacaacggc tcggccacca cctcggcgcc
      121 gacccactcg ccgaacacgg ccacgtccac gcgcgcgccc tcctcgacct cggacggcag
      181 gtaggcgaag gcgatgctgc gcgagacccg gtggccgtag ccgcccgagg tcacgcgccc
      241 gaccggcgac ccgtccacgc gcaccggctc gctgcccagg cacaccgacc gcgggtcgtc
      301 cagcacaagg cagcgcagcc gctgcgccgg accgtcgccc gcgcgcgcgg cggccagcgc
      361 gtcgcggccg atgaacccgc cctccttgtc catggccacg gcgaacgaca ggcccgcctc
      421 gtcgggcgtg gtctcgggcg tgatgtccag gccccagacg cggtagccct tctccaggcg
      481 catcgagtcg atggcgcggt acccgccgcc caccaggccg tgctcgcgcc cggcctgcca
      541 cagcaggtcc cacagcgtca ggccgaactc ggcggacgcg tacagctccc agcccagctc
      601 gccgacgaac gtgatccgct gggcgaacac cggcaccggc ccgacggaga tctcgcgcgc
      661 gcgcaggaac ttgaacgcca ggtcgtcgtc ggtcagcgac gacaggatct cccgcgccgc
      721 ggggccccag aggcagtagc agcagcgcga cccggtcacg tcctccgccg tcaccgaccc
      781 gtcgcgcggc aggtgcctgc ggatccacgc aaggtcgtgt cgcccgaagg cggtcccggt
      841 gaccacgcgg aacgactcct cgcccacgcg ggtcacggtc aggtcggcct cgatgccgcc
      901 gccgtcgttg agcagctggg tgtacacggc gcggcccacg tcgccggcca cgtcgttggc
      961 gcacagccgc gacagcgcgg ccgccgcgcc gggcccgcgc acgtcgatct tggcgaacga
     1021 cgactggtcg aacagcgcgg ccgtgtcgcg cgcggccagg cactcggcgc cgatggccgg
     1081 cgaccagaac tcgcccgccc acccgcgcgg gcgcagcgac tcgtcgcccg cgccggcgtt
     1141 ggagccgaac cagttcacgc gctcccaccc ggccttctcg ccgaactcgg cgtccagcgc
     1201 ggccaggcgc gggtaggcgg gggacaggcg cagcggccgc ccggcgtcga actcctggtg
     1261 cgggtagacg atgtcgtagt agcgcgagta cgcgctcagc gcgcgcgtgc gcgagaacga
     1321 cctggacccg tagtgcgccc cgaagcggcg gatgtccatc gaggccatgt cgaactcggg
     1381 ccgcccgtcg gtgatccact cggccatgac cttgcccacg ccgccggcgc cggccagccc
     1441 gtgcacgcag aacccggcgg cggtccagag gccctcgacc tcggtctcgc ccaggatgaa
     1501 gtcgccgtcg ggggtgaagg cctcgggcgc gttgaccacc ttggccggct cgagcgagcg
     1561 cagcgccggc acgcggttgc gcgcgccctc ccacgactcg gcgaagcgct ccatgtccgg
     1621 gtcgaacgtg gtccgcggct ccgccagcgg cgaggcggtg tcccacgtga ccggctcgcg
     1681 cgagtagccg cccaccagca gcccgcccga gtcgtgcggg cggaagtaca tgatgtggtc
     1741 ggggtcgcgg acggtggcga tgccctccac gtcctccgcc agcggctccg tgaccacgta
     1801 ctggtggcgc atgggggtga tggggatgac cgcacccgcc atggcgccca ccacgccggt
     1861 ggcggcgccc gcggcgatga ccaccacctc ggcggccacc ggcccggccg aggtctggac
     1921 gcccgtgacg cgcccgccgt ccacgccgat gcccgtcacg gtcacgccgg tgtgcacggc
     1981 cacgccctgg cgccgcgcgc cgtcggccag ggccagcgcc agcaactcgg ggtccacgta
     2041 gcc
//
LOCUS       DASUPW010000390         3965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_508333, whole genome shotgun sequence.
ACCESSION   DASUPW010000390 DASUPW010000000
VERSION     DASUPW010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508333"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1040)
                     /locus_tag="VFY44_08755"
     CDS             complement(72..1040)
                     /locus_tag="VFY44_08755"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5910569.1"
                     /translation="MRVTVTGGLGYIGACAVEELVDAGNEVRVLDSLLHSQEDIASHV
                     EGLGVELIRGDVRDGDARQKAFEGADAVIHLAAIVGDPACAKDPELSNDVNIQGSRGV
                     VADAQAAGVGRLVFASTCSNYGRMADPTVPITEDGELAPVSLYAEQKVEIEKSLLEGD
                     FGDLKPTCLRFATVYGVAPRMRFDLTVNEFTRDLWADRELEVFGEQFWRPYVHVRDAG
                     RGVALVLGSDLDKVAGRVFNVGHSDENYRKLDLVEIITGQLGRGNVSYVTKNEDPRDY
                     KVGFERIKDELGFEPAQRVPEGIAELIEALGNDAFEDPWGPKYRNT"
     gene            complement(1113..2189)
                     /locus_tag="VFY44_08760"
     CDS             complement(1113..2189)
                     /locus_tag="VFY44_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910570.1"
                     /translation="MNFVKKFNQSESDRFTQRPSAQQKAFFRQNYKGMIVSSPYFDSR
                     LSFYPDAWVYRSLYGVFPGSATVRKHPSWFLRTARRGTKAYIPFDCSGGTCPQLAGDI
                     TNPRFRKAWIREARTQVRRGYKSVFIDEVNMIRRVGTGTGRELAPYSPYLRRKITVRR
                     WRTAVASFTRQIRRSLPRRVRIVHNVIWFAPGKKDRLTRMQMRAATVVALERGVNDAG
                     LTGGRGPFGVSTFLNYVSFIHRLKRPVIFIEDAPNADARNYGLAGYLLINNGRDFLSS
                     DPFGEPDKFWPGYRTGLGKAKGGRYRWRGLIRRNFERGIVLLNEPQAPSRTVALGGNY
                     RQVGTSTQQSSITLGASTGAVLVR"
     gene            complement(2331..3767)
                     /locus_tag="VFY44_08765"
     CDS             complement(2331..3767)
                     /locus_tag="VFY44_08765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HEX5910571.1"
                     /translation="MGSRLDPQRLFEGPGWRNLRVGADLIALVVAAPLAALAAAPGDL
                     GDDGTTLLWFLGPVAMALMAIWGLYASRTQPKIMDAVGKIIAATSLATIILIAGAAFI
                     EPNSQPAPLLARGWLYGTLFLIGTRLLLNWAQSRGRAAGLVATPTLIVGAGVVSSHVE
                     MRLTTQPRLGLLPVGYLDANPPPEEMVPERTAPVLGTPDDLERVVSETGARHVVFGFL
                     SGPDSEVLPMMRRCEALGLQVSFVPRFFESVNVHLALEHLGGLPLFGLRPIDPKGWQF
                     AIKHVFDRLLAGLLLVLLAPLLAALALATRLSSPGPVLFRQRRIGRDGHGFDMLKFRS
                     MRLAPAPMEMTTTAGQVVALPADVAPGGVEGDDRRTWVGTLMRSTSLDELPQLVNVLR
                     GDMSLVGPRPERPEFVELFGSRIDRYEDRHRVKSGVTGWAQVHGLRGKTSLRDRVEWD
                     NYYIENWSLGLDFKILVMTVAAILQRAE"
BASE COUNT          583 a         1302 c         1492 g          588 t
ORIGIN      
        1 cgaaggagcc gcggtcgtga tcgtgccggt catggccggt ccgatcgtga ccgcggcgcc
       61 ccgggagcgc ctcaggtgtt gcggtacttc ggaccccacg ggtcctcgaa ggcatcgttg
      121 ccgagggcct cgatcagctc ggcgattccc tcgggcacgc gctgtgcggg ctcgaagccc
      181 agctcgtcct tgatccgctc gaagccgacc ttgtagtcgc gcgggtcctc gttcttggtg
      241 acgtaggaga cgttgccgcg gcccagctgg ccggtgatga tctcgaccag gtcgagcttg
      301 cggtagttct cgtcggagtg gcccacgttg aacacgcggc cggcgacctt gtccaggtcg
      361 gagcccagga ccagggcgac gccgcggccg gcgtcgcgga cgtggacgta ggggcgccag
      421 aactgttcgc cgaagacctc gagctcgcgg tcggcccaga ggtcgcgggt gaactcgttg
      481 acggtcaggt cgaagcgcat ccggggcgcg acgccgtaga cggtggcgaa gcgcaggcag
      541 gtgggcttga ggtcgccgaa gtcgccctcg agcagggact tctcgatctc caccttctgc
      601 tcggcgtaca gcgacacggg ggccagctcg ccgtcctcgg tgatcgggac ggtcgggtcg
      661 gccatgcggc cgtagttgga gcaggtggag gcgaacacca gccggccgac gccggcggcc
      721 tgggcgtcgg ccacgacgcc gcggctgccc tggatgttca cgtcgttgga gagctcgggg
      781 tccttggcgc aggcggggtc gccgacgatg gcggccaggt ggatgaccgc gtcggcgccc
      841 tcgaaggcct tctggcgcgc gtcgccgtcg cggacgtcgc cgcggatgag ctcgacgccg
      901 aggccctcga cgtggctggc gatgtcctcc tgggagtgca ggagggagtc cagcacccgc
      961 acttcgttac cggcgtcgac cagctcttcg acggcgcacg cgccgatgta gcccagtccg
     1021 cccgtgaccg ttactcgcat tccgtcagca gcccttcgtc gtttgacgtg tcagcggtgc
     1081 atcggcagcc atgccgaacc ccttgaggaa ggtcagcgga ccagcacggc gccggtggag
     1141 gcgccgagcg tgatcgagga ctgctgcgtg gaggtgccca cctggcggta gttgccgccc
     1201 agggcgaccg tgcgcgaggg cgcctgcggc tcgttgagca gcacgatgcc gcgctcgaag
     1261 ttgcgccgga tcaggccccg ccagcggtag cggccgccct tggccttgcc cagcccggtc
     1321 cggtagccgg gccagaactt gtcgggctcg ccgaaggggt cgctgctgag gaagtcgcgc
     1381 ccgttgttga tgagcaggta gccggccagc ccgtagttgc gggcgtcggc gttgggggcg
     1441 tcctcgatga agatcacggg ccgcttgagc cggtggatga acgagacgta gttcaggaac
     1501 gtgctgacgc cgaaggggcc gcgaccgccg gtcaggccgg cgtcgttgac gccgcgctcg
     1561 agcgccacca cggtggcggc ccgcatctgc atccgggtca gccggtcctt cttgcccggc
     1621 gcgaaccaga tcacgttgtg cacgatccgc acgcgccgcg gcagcgagcg ccggatctgg
     1681 cgcgtgaagc tcgcgacggc tgtgcgccac cggcgcacgg tgatcttccg gcgcaggtag
     1741 gggctgtagg gcgcgagctc gcggccggtg cccgtgccga cgcggcggat catgttcacc
     1801 tcgtcgatga acacggactt gtagccgcgc cggacctggg tccgcgcctc gcggatccac
     1861 gccttgcgga agcgcgggtt ggtgatgtcg ccggccagct gggggcacgt gccgcccgag
     1921 cagtcgaacg ggatgtaggc cttggtgccg cggcgcgcgg tgcgcaggaa ccagctgggg
     1981 tgcttgcgca cggtcgcgga cccggggaag accccgtaca ggctgcggta gacccaggcg
     2041 tcggggtaga acgacagccg tgagtcgaag taggggctgg agacgatcat gcccttgtag
     2101 ttctggcgga agaacgcctt ctgctgggcg gacggccgct gggtgaagcg atcggactcc
     2161 gactggttga acttcttcac gaagttcacg cttccggagg gtgccgccga cgcgctcccg
     2221 gcgaagccga gagcgagcat cagagcgacg cacgagagcg ccgcggacag ggcgattcgc
     2281 ccctgccgtg cggtgtccac ctgccggacc aaggcgcgca ctctagctag ttattcagcg
     2341 cgctgaagga tggccgccac ggtcatgacg aggatcttga agtccaggcc cagcgaccag
     2401 ttctcgatgt agtagttgtc ccactccacc cggtcgcgga gcgaggtctt gccgcgcagg
     2461 ccgtgcacct gggcccaccc ggtgacgccg gacttgaccc ggtggcggtc ctcgtagcgg
     2521 tcgatgcggg agccgaagag ctcgacgaac tcgggccgct cggggcgcgg gccgaccagg
     2581 ctcatgtcgc cccgcaggac gttgaccagc tgcggcagct cgtccagcga ggtcgagcgc
     2641 atcagcgtgc ccacccaggt gcggcggtcg tcgccctcca caccgccggg ggccacgtcg
     2701 gccggaaggg cgaccacctg gccggcggtg gtggtcatct ccatcggcgc gggggccagc
     2761 cgcatcgagc ggaacttgag catgtcgaag ccgtggccgt cgcggccgat gcggcgctgg
     2821 cggaacagca ccgggcccgg cgagctcagg cgcgtggcca gggccagggc ggccagcagg
     2881 ggggcgagca gcacgagcag gaggccggcc aggagccggt cgaacacgtg cttgatggcg
     2941 aactgccagc ccttggggtc gatcggcctc aggccgaaca gcggcagacc gcccaggtgc
     3001 tccagcgcca ggtggacgtt cacgctctcg aagaagcgcg gcacgaacga gacctgcagg
     3061 ccgagggcct cgcagcggcg catcatcggc agcacctcgc tgtcgggtcc ggagaggaag
     3121 ccgaacacca cgtggcgggc gccggtctcg ctgacaaccc gctccaggtc gtcgggggtg
     3181 cccaggacgg gcgcggtgcg ctcgggcacc atctcctcgg gcggcgggtt ggcgtccagg
     3241 tagccgaccg gcagcaggcc cagccgcggc tgggtggtca ggcgcatctc gacgtgggag
     3301 ctgaccacgc cggcgccgac gatcagggtg ggggtggcca ccaggccggc ggcgcggccg
     3361 cggctctggg cccagttcag cagcagccgg gtgccgatca ggaagagcgt gccgtacagc
     3421 cagccccggg ccagcagggg cgccggctgg ctgttgggct cgatgaaggc ggcgccggcg
     3481 atcaggatga tcgtggccag cgacgtggcg gcgatgatct tgccgaccgc gtccatgatc
     3541 ttcggctgtg tccggctggc gtacagcccc cagatggcca tgagggccat ggcgaccggt
     3601 cccaggaacc acagcagcgt ggtgccgtcg tcgcccaggt ccccgggcgc cgcggccagc
     3661 gcggccaggg gcgcggccac cacgagggcg atcaggtccg cgccgacgcg gagattgcgc
     3721 cagccgggcc cttcgaaaag gcgctggggg tcgaggcggg aacccatctt tgactctttt
     3781 cggtccgccg ggacccctgc atgagtgagg ccggtggata tttcatccac cggcctcctc
     3841 gaatcctctg cggttgcgtg ctccatcatc ggtggagaca cggtaacgcc gcaggcgttg
     3901 cgcccccgct aaagggcgct atgtgtcacg ggacccgctc agacggattc gggtgcgcgc
     3961 gtgag
//
LOCUS       DASUPW010000391         2550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_2032550, whole genome shotgun sequence.
ACCESSION   DASUPW010000391 DASUPW010000000
VERSION     DASUPW010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2032550"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VFY44_08770"
     CDS             <1..598
                     /locus_tag="VFY44_08770"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5910572.1"
                     /translation="ADVTDEQQVAGMYGRTKQEFGRVDVIVNNAGINPPEDMSVLDTP
                     VETWQWVQDVNVRSVFLCCKHGIPHLLEAGGGSVINTASFVAVMGAATSQISYTASKG
                     AVLSMSREMGVEFARRGVRVNALCPGPVDTPLLQELFASDPAKAARRMVHVPPGRFGE
                     AREVANAALFLASDESSYVNATTFLVDGGLSGAYVTPE"
     gene            782..1678
                     /locus_tag="VFY44_08775"
     CDS             782..1678
                     /locus_tag="VFY44_08775"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX5910573.1"
                     /translation="MELADRQEGLLSSDQARACGVSRKVVAARIRRGIWRSPHRRVIA
                     VDRREVKPRARVLAAVLACRAPGAVASHSTAAWLWGLLERWPGLVHLTALGGRNPGQL
                     DGTRIHRPERPPERIRWHNGIPLTTPADTLLDVAGLLGLDELEASVARALRGGTIRRA
                     DLRRAIAHAGPRPGIANLRAAARDPDLTRSGNERLLVALLRRAELTGFETNVEVEGKE
                     VDVYWPEARLAIEVDSYATHGDEATYEDDHVLDADFEAAGIKLLRFTGRRIRARPEAV
                     LARITAPLALRLGGLPVPRRRR"
     gene            1750..1974
                     /locus_tag="VFY44_08780"
     CDS             1750..1974
                     /locus_tag="VFY44_08780"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HEX5910574.1"
                     /translation="MERKMADCRRFESDANCSLTIIGDEDEVVETAVMHAVAAHGHEE
                     SDEMRRQIRENLESEADYRPGAREPEPFPG"
     gene            complement(1971..>2550)
                     /locus_tag="VFY44_08785"
     CDS             complement(1971..>2550)
                     /locus_tag="VFY44_08785"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEX5910575.1"
                     /translation="AAANVDDTEANPWMAEDLRVVAELLARGVPTLGVCLGAQLVAKA
                     AGGSVERAAAPEIGWYEVALEPGAEEDPLAGGLPERFVSFQWHSYVAAPPDGSITLAR
                     SAHCLQAFRLADAPAWGIQFHAEVSPADAAAWTRQYDHDEDAVAMGLDPVALQAETDR
                     RIEGWNQLGRDLFARFLAVGALPPERPPRTPA"
BASE COUNT          341 a          923 c          959 g          327 t
ORIGIN      
        1 cgccgacgtg accgacgagc agcaggtggc cggcatgtac gggcgcacca agcaggagtt
       61 cgggcgcgtg gacgtgatcg tcaacaacgc cggcatcaac ccgcccgagg acatgagcgt
      121 gctggacacc ccggtcgaga cctggcagtg ggtgcaggac gtgaacgtgc gcagcgtgtt
      181 cctgtgctgc aagcacggca tcccgcacct gcttgaggcc ggtggcggat cggtcatcaa
      241 caccgcctcg ttcgtggccg tcatgggcgc cgcgacgtcg cagatctcct acacggcgtc
      301 gaagggcgcg gtgctgtcga tgtcgcgcga gatgggcgtg gagttcgcgc gccgcggggt
      361 gcgcgtgaac gccctctgtc ccgggccggt ggacacccca ctgctccagg agctgttcgc
      421 ctccgacccc gccaaggcgg cgcgccggat ggtgcacgtg ccgccggggc gcttcggcga
      481 ggcgcgcgag gtggccaacg ccgcgctgtt cctggcctcc gacgagtcca gctacgtgaa
      541 cgccaccacc ttcttggtgg acggcggcct gtcgggcgcc tacgtgacgc ccgagtaggg
      601 ggcgaacgtc tgccgccccg gcccttgagg gggtgtgggc gggccatctg acgacggacg
      661 accttcgact cccgagctcc gtcgggaggc ggcccggctt gggccgcccg acgtcacggc
      721 gcgccttcag gatgccgccc gtcgcacggc ggccctgttc gggccgggcg aacgtgcggt
      781 ggtggagctg gccgaccggc aggagggact gctttcgtcc gatcaggccc gggcctgtgg
      841 cgtcagccgc aaggtggtgg ccgcccggat ccggcgcggc atctggcggt cgccgcaccg
      901 gcgcgtgatc gcggtcgacc gtcgcgaggt caagccccgg gctcgtgtgc tcgccgccgt
      961 cctggcctgt cgcgccccgg gcgccgtggc cagccactcg accgcggcgt ggctgtgggg
     1021 cctgctggag cggtggccgg ggctggtcca cctgacggcg ctcggaggcc ggaaccccgg
     1081 gcagctggac ggcacccgga tccaccggcc ggagcggccg cccgaacgga tccgctggca
     1141 caacggcatt ccgctgacga ccccggccga cacccttctg gacgttgccg gactgctcgg
     1201 cttggacgag ctcgaggcca gcgtcgcccg ggcgcttcgc ggcggcacca tcaggcgagc
     1261 ggacctgcgc cgggccatcg cccacgccgg cccgcgcccc ggaatcgcca acctccgcgc
     1321 cgcggcccgg gaccccgacc tcacccgctc gggcaacgaa cggctgctgg tcgcgctcct
     1381 gcgccgcgcc gagctgaccg ggttcgagac caacgtggag gtcgagggca aggaggtcga
     1441 cgtctactgg ccggaggccc ggctggcgat cgaggtggac agctacgcca cgcacggcga
     1501 tgaggccacg tacgaggacg accacgtgct ggacgccgac ttcgaagccg ccggcatcaa
     1561 gctgctgcgc ttcaccggcc ggcggatccg ggctcggccc gaggccgtgc tggcccgcat
     1621 cacggcgccg ctggcgctac ggctcggtgg cctgcccgtg ccccggcggc gccggtgacc
     1681 gaacttgacc gccgccccgg ggcgggcgta gcgtgggaag gtcacctggc ccggaaggag
     1741 gtgaggacga tggagcgcaa gatggccgac tgccgtcgct tcgagagcga cgccaactgc
     1801 tcgctcacca tcatcggtga cgaggacgag gtggtcgaga ccgccgtgat gcacgcggtg
     1861 gccgcgcacg gccacgagga gtcggacgag atgcgccggc agatcaggga gaacctggag
     1921 tccgaggcgg actaccgccc cggtgcgcgc gagccggagc cgttccccgg ctaggccggc
     1981 gtacgtggtg ggcgttccgg cgggagggcg cccaccgcca ggaagcgcgc gaacaggtca
     2041 cggccgagct ggttccagcc ctcgatcctc cggtcggtct cggcctggag cgccaccggg
     2101 tccaggccca tggccacggc gtcctcgtcg tggtcgtact gccgcgtcca cgcggccgcg
     2161 tcggccgggg acacctcggc gtggaactgg atgccccagg cgggcgcgtc cgcgagccgg
     2221 aaggcctgga ggcagtgggc gctgcgcgcc agcgtgatcg acccatcggg cggggccgcc
     2281 acgtacgaat gccactggaa gctcacgaac cgctcgggca gcccgccggc gagcgggtcc
     2341 tcctcggcgc cgggctccag cgccacctcg taccagccga tctcgggggc ggcggcgcgc
     2401 tccaccgacc cgccggcggc cttggccacc agctgcgcgc ccaggcacac gcccagggtg
     2461 ggaacgccgc gcgccagcag ctcggcgacc acgcggaggt cctccgccat ccacgggttc
     2521 gcctccgtgt cgtccacgtt ggcggcggcg
//
LOCUS       DASUPW010000392         2240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_923057, whole genome shotgun sequence.
ACCESSION   DASUPW010000392 DASUPW010000000
VERSION     DASUPW010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923057"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VFY44_08790"
     CDS             <1..536
                     /locus_tag="VFY44_08790"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5910576.1"
                     /translation="QATGQRASHVWAELPVPVIAAIHGNALGGGLQIALGADIRISAP
                     DATLSVMESRWGLIPDMGITRALPRLVGIDVAKELTLTARRFDGAEADRLGVVTRTAG
                     DPHAAAQELAAEIAQRSPDAVRGAKRLFDESWTGDAADTLALEEQIQRTLMGSPNQMA
                     AVMAGVSKQPAEFADPA"
     gene            577..1020
                     /locus_tag="VFY44_08795"
     CDS             577..1020
                     /locus_tag="VFY44_08795"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5910577.1"
                     /translation="MDAPRRDAWDVLADCESWPAWWQGVEAVDEIAAGDARRVGSAYR
                     VTWRAPVVPYRVRFDFHVDTVDEPRSMAGHADGALQGAGTWRLFEDEGVCAVTFDWEV
                     RTKRAWMNALAPLARPVFTRSHDRLMSRGGHDLARRMGVRLLAAG"
     gene            complement(1017..>2240)
                     /locus_tag="VFY44_08800"
     CDS             complement(1017..>2240)
                     /locus_tag="VFY44_08800"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5910578.1"
                     /translation="KRAEARKREFDRFRTLNGRLSGNLAAERKRELGRAGAVSAGVTI
                     LLALLFGGLGYVVIERQARRARERREAERRYRETQSEFAETIQVMQDEDEAHALVKHH
                     LEREIDGACATALVRNNSDDRLVAATPVDPDSPLAAGLVDASPDTCLAVRLARPYDQG
                     QDSAPLLPCKLCGKSAPEVTCVPSLVSGEVIGSLLVQRDTPLEARHRERVVETIGQAS
                     PVLANLRNLAIAETRASTDALTGLPNSRSCRDNLKRMVAAAGRTLAPCTAILLDLDHF
                     KQINDRFGHGAGDDVLAAVGDVMRSTLRDSDFAGRYGGEEFLMLLPDTDQEGGLEAAE
                     KVREAIQGLELAQVDRAITASLGVASFPGDAMDSDTLVRQADRALYAAKGAGRNRVML
                     AMPGATPQAEQHTGR"
BASE COUNT          276 a          810 c          853 g          301 t
ORIGIN      
        1 cgcaggccac cgggcagcgg gcgtcccacg tctgggcgga gctcccggtc ccggtgatcg
       61 cggcgatcca cggcaacgcg ctcggcggcg gcctgcagat cgcgctgggc gccgacatcc
      121 gcatcagcgc gcccgacgcg acgctgtcgg tgatggagtc gcgctggggc ctgatccccg
      181 acatgggcat cacccgggcg ctgccgcggt tggtgggcat cgacgtcgcc aaggagctga
      241 cgctgaccgc gcgccggttc gacggggccg aggccgaccg gctgggcgtg gtcacgcgca
      301 cggccggcga cccccacgcc gcggcgcagg agctggcggc cgagatcgcg cagcgctcgc
      361 ccgacgccgt gcgcggcgcg aagcgcctgt tcgacgagtc gtggaccggc gacgccgccg
      421 acacgctggc gctggaagag cagatccagc gcacgctgat gggctcgccc aaccagatgg
      481 cggcggtcat ggccggcgtg tccaagcagc cggccgagtt cgcggacccg gcctgaggcc
      541 ggcacgccct cctaccgctt cctgaccacc tggctgctgg acgccccgcg ccgggacgcg
      601 tgggacgtgc tggccgactg cgagtcctgg cccgcgtggt ggcagggggt cgaggccgtg
      661 gacgagatcg cggcgggcga cgcgcgccgc gtgggcagcg cctaccgcgt gacctggcgc
      721 gcgccggtgg tgccctaccg cgtgcgcttc gacttccacg tggacacggt cgacgagcca
      781 cggtcgatgg cgggccacgc ggacggcgcc ctccagggtg cgggcacctg gcggctgttc
      841 gaggacgagg gcgtgtgcgc ggtgaccttc gactgggagg tccgcaccaa acgtgcgtgg
      901 atgaacgctc tggcgccgct ggcgcggccc gtgttcacac ggagccacga ccggctgatg
      961 tcccgcggcg gccacgacct ggcgcggcgg atgggggtgc ggctgctggc cgcgggctag
     1021 cggcccgtgt gctgctcggc ctgcggggtg gcgccgggca tggccagcat gacccggttg
     1081 cggccggccc ccttggccgc gtacagcgcg cggtcggcct ggcgcaccag ggtgtcggag
     1141 tccatggcgt cgccggggaa cgaggccacc ccgaggctgg ccgtgatcgc gcgatccacc
     1201 tgagcgagct ccaggccctg gatcgcctcg cgcactttct cggccgcctc cagcccaccc
     1261 tcctggtcgg tgtccggcag cagcatcagg aactcctcgc cgccgtaacg gccggcgaaa
     1321 tcgctgtcgc gcagcgtgct gcgcatgacg tcgccgacgg cggccaggac atcgtcgccc
     1381 gcgccgtgcc cgaagcggtc gttgatctgc ttgaagtggt cgaggtccag caggatcgcc
     1441 gtgcacggcg ccagcgtccg gccggcagcc gccaccatgc gcttgaggtt gtcgcggcac
     1501 gagcgcgagt tggggagccc ggtcagggcg tcggtcgagg cgcgcgtctc ggcgatggcc
     1561 aggttgcgca ggttggccag cacgggcgag gcctggccga tggtctccac gacccgttcg
     1621 cggtgcctgg cctccagcgg cgtgtcgcgc tggaccagca acgacccgat gacctcgccg
     1681 ctgaccagcg acggcacgca cgtgacctcg ggggccgact tgccgcacaa cttgcagggc
     1741 aacagcggcg ccgagtcctg gccctggtcg taggggcggg ccagccgcac ggccaggcag
     1801 gtgtccggag acgcatccac cagcccggcg gccagcggcg agtccgggtc cacaggcgtc
     1861 gcggccacga gcctgtcgtc ggagttgttg cgcaccagcg cggtggcgca cgcgccgtcg
     1921 atctcgcgct cgaggtggtg cttgaccagc gcgtgggcct cgtcctcgtc ctgcatgacc
     1981 tggatcgtct cggcgaactc gctctgggtc tcgcggtacc ggcgctcggc ctcgcggcgc
     2041 tcgcgggcac ggcgcgcctg gcgctcgatg accacgtagc ccagcccgcc gaacagcagc
     2101 gccagcagga tggtcacgcc ggccgacacc gcgcccgcgc ggccgagctc gcgcttgcgc
     2161 tccgccgcca ggttgccgct gaggcggccg ttgagcgtgc ggaagcggtc gaactcgcgc
     2221 ttgcgggcct cggcccgctt
//
LOCUS       DASUPW010000393         3244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_784836, whole genome shotgun sequence.
ACCESSION   DASUPW010000393 DASUPW010000000
VERSION     DASUPW010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784836"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VFY44_08805"
     CDS             complement(<1..447)
                     /locus_tag="VFY44_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5910579.1"
                     /translation="MSTVPTLDVRADFPVLRDGPGAGERGLAYLDSAATSQKPDAVID
                     AMAGFNRGSYGTVHRSVYALAERSTELFEGARTKAAELVSWEPDTTIFTANATAAINL
                     VAYTWARQNLEPGDQIVLTQMEHHSNIVPWQLAAAERGATLRWLHVS"
     gene            complement(444..1772)
                     /locus_tag="VFY44_08810"
     CDS             complement(444..1772)
                     /locus_tag="VFY44_08810"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HEX5910580.1"
                     /translation="MPASWLEEQREIALKAFEAEEVPTWRRSGFWTTSLRGLDLDALE
                     ARRYEPLADGDALPPTVAEDIGDSELGGLIVQRGATTIRSWIDPEVEAQGVIVCSLEE
                     AVDKHPDEVREFYGKRLDPSEGKFTAASLAFWTGGAFVWVPKDVKVEKPIQIVYLIDE
                     PGTAQYAQTLVVIGEHSQASIREYDLGTPFEGQALHAGGFELFARTGARADVAHFQDW
                     GPAEVYDISTKRVEVGRDAFVSWVPIHLGGRLTKQTLDIITAEKGSDMRHTGMYFTEG
                     EEHLDLFTTDRHEAGHTGGDTVWKGALTGSSRASYEGLIEIIENCQETHTYLQTHQML
                     LSSKAKGDAIPSLIVKTDSVSASHGGTVGELDEEQIFYMMTRGISRSEAVRVLVEGYF
                     EEVVQRLHDEQLEALVRRRIAQKLLAAEDQVNEFIGERVSLEEAEGAQAP"
     gene            complement(1762..2514)
                     /gene="sufC"
                     /locus_tag="VFY44_08815"
     CDS             complement(1762..2514)
                     /gene="sufC"
                     /locus_tag="VFY44_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX5910581.1"
                     /translation="MSLEIKNLHVRTEEREILHGVDLKVAKGETHALMGPNGSGKSTL
                     ANTVMGNPVYEVTEGQILFNGEDLTEASPDERARAGLFLAFQYPATIPGVSVANFLRM
                     AVNARRDEPMKVKDFGKYMRENVELLKIDPEFVSRYLNEGFSGGEKKRAEILQLAMLR
                     PEIAVLDETDSGLDIDALRIVSEGVNKLRGPEMGSLIITHYTRILNYVKPDFVHILLD
                     GRIVREGGSELADELEEKGYDFIRAEVAANAG"
     gene            complement(2511..2981)
                     /locus_tag="VFY44_08820"
     CDS             complement(2511..2981)
                     /locus_tag="VFY44_08820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX5910582.1"
                     /translation="MFSTKAEYGVRVMAHLAGRSDSDGPVSLHAIAEAEGLPQAYLEH
                     LAARLRKAELVQSHRGARGGYTLARPAGDITMAEVVRALEGDIAPIECITSDADGALT
                     CVREGDPSHQPCPTKLLWTRVQGSIVRTLTDMTLADLARPAPAPIPTEPETVNA"
BASE COUNT          437 a         1115 c         1157 g          535 t
ORIGIN      
        1 ggacacgtgc agccagcgca gcgtggcgcc cctctcggcc gcggccagct gccacggcac
       61 gatgttggag tggtgctcca tctgggtcag cacgatctgg tcgcccggct ccaggttctg
      121 gcgcgcccac gtgtaggcca ccaggttgat cgccgcggtg gcgttggcgg tgaagatcgt
      181 ggtgtcgggc tcccacgaga ccagctcggc cgccttggtc cgggcgccct cgaacagctc
      241 ggtcgagcgc tcggccagcg cgtacacgct gcggtgcacg gtcccgtacg agccgcggtt
      301 gaagccggcc atcgcgtcga tgaccgcgtc gggcttctgc gacgtggccg cggagtccag
      361 gtaggccagc ccgcgctcgc ccgcgcccgg cccgtcccgg agcaccggga agtccgcgcg
      421 gacgtcgagc gtgggcacag tgctcacggc gcctgggccc cttcggcctc ttccaggctg
      481 acgcgctcgc cgatgaactc gttgacctgg tcctcggcgg ccagcagctt ctgggcgatc
      541 cggcgccgca ccagcgcctc cagctgctcg tcgtgcaggc gctggacgac ctcctcgaag
      601 tacccctcga ccagcacccg gacggcctcc gagcgcgaga tgccgcgggt catcatgtag
      661 aagatctgct cctcgtcgag ctcgccgacg gtgccgccgt gcgaggccga cacggagtcg
      721 gtcttcacga tcagcgaggg gatggcgtcg cccttggcct tcgagctcag cagcatctgg
      781 tgggtctgca ggtaggtgtg cgtctcctgg cagttctcga tgatctcgat caggccctcg
      841 tatgaggcgc gcgaggagcc ggtcagggcg cccttccaca cggtgtcgcc gccggtgtgg
      901 ccggcctcgt ggcgatcggt ggtgaacagg tccaggtgct cctcgccctc ggtgaagtac
      961 atgcccgtgt ggcgcatgtc cgaccccttc tcggcggtga tgatgtccag cgtctgcttg
     1021 gtcaggcggc cgcccaggtg gatcggcacc cacgagacga acgcgtcgcg gccgacctcg
     1081 acccgcttgg tcgagatgtc gtagacctcg gccgggcccc agtcctggaa gtgggccacg
     1141 tcggcgcggg cccccgtgcg ggcgaacagc tcgaacccgc cggcgtgcag cgcctggccc
     1201 tcgaacggcg tgcccaggtc gtactcgcgg atcgaggcct gcgagtgctc gccgatgacc
     1261 accagcgtct gcgcgtactg cgcggtgccg ggctcgtcga tcaggtagac gatctggatc
     1321 ggcttctcga ccttgacgtc cttgggcacc cacacgaacg ctccaccggt ccagaaggcc
     1381 agcgaggcgg cggtgaactt gccctcggag gggtccaggc gcttgccgta gaactcgcgc
     1441 acctcgtccg ggtgcttgtc gaccgcctcc tccagcgagc agacgatcac gccctgggcc
     1501 tcgacctcgg ggtcgatcca ggagcggatg gtggtggccc cgcgctggac gatcaggccg
     1561 cccagctcgg agtcgccgat gtcctcggcc acggtggggg gcagggcgtc gccgtcggcc
     1621 agcggctcgt agcggcgcgc ctcgagcgcg tccaggtcca gcccgcgcag cgacgtggtc
     1681 cagaagcccg agcggcgcca cgtgggcacc tcctcggcct cgaaggcctt cagcgcgatc
     1741 tcgcgctgct cctcgagcca gctagccggc attggcggcc acctccgcgc ggatgaagtc
     1801 gtagcccttc tcctcgagct cgtcggccag ctcggagccg ccctcgcgca cgatccgacc
     1861 gtccagcagg atgtgcacga agtcgggctt cacgtagtta aggatgcgcg tgtagtgcgt
     1921 gatgatcagc gagcccatct cgggcccgcg gagcttgttc acgccctcgg agacgatccg
     1981 cagcgcgtcg atgtccaggc cggagtcggt ctcgtccagc acggcgatct cggggcggag
     2041 catggccagc tgcaggatct cggcgcgctt cttctcgccg cccgagaagc cctcgttcag
     2101 gtaccggctg acgaactccg ggtcgatctt cagcagctcg acgttctcgc gcatgtactt
     2161 gccgaagtcc ttgaccttca tcggctcgtc gcggcgggcg ttcacggcca tgcgcaggaa
     2221 gttggccacc gagacgccgg ggatcgtggc cgggtactgg aaggccagga acaggccggc
     2281 gcgggcgcgc tcgtcggggg aggcctcggt caggtcctcg ccgttgaaca ggatctggcc
     2341 ctcggtgacc tcgtacacgg ggttgcccat gacggtgttg gccagcgtcg acttgcccga
     2401 gccgttcggg cccatcagcg cgtgggtctc gcccttcgcg accttcaggt cgacgccgtg
     2461 gaggatctcg cgctcctccg tgcggacgtg caggttcttg atctcgaggc tcatgcgttc
     2521 acggtctccg gttcggtcgg gatgggggcc ggcgcaggtc gcgccaggtc cgccaaggtc
     2581 atgtcggtca gggtgcggac gatcgagccc tggactcggg tccacagcaa cttggtgggg
     2641 cagggctggt gcgagggatc gccctcgcgc acgcacgtca gtgcgccgtc ggcgtcgctg
     2701 gtgatgcact cgatcggggc gatgtcgccc tccagcgcgc gcacgacctc ggccatcgtg
     2761 atgtccccgg cgggccgcgc cagcgtgtag ccgccgcggg cgccgcgatg gctctgcacc
     2821 agctcggcct tccgcagccg cgcggccagg tgctccaggt acgcctgggg cagcccctcg
     2881 gcctccgcga tcgcgtgcag ggagacgggc ccgtcgctgt cggagcgacc ggccaggtgc
     2941 gccatcacgc gcaccccgta ctcggctttg gttgagaaca tcatcgatcg gatctcggcg
     3001 gggaatacct accgccggac taggttaacg atggccgcag gaatcgttcg cggagaaccg
     3061 gcgcagagcc gtaccggtcg tgaatagggt ccgcggccat gagggggcga ctgggggttc
     3121 tgggggcgac ggtcgtggct gcgctggcat tcgccggccc tgccgaggcg cagttcaagc
     3181 ggtgccagaa gggggagcgc gccgagtgcg ccgagctccg ggtcccgctg gaccgggcgg
     3241 gcca
//
LOCUS       DASUPW010000394         2973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1755109, whole genome shotgun sequence.
ACCESSION   DASUPW010000394 DASUPW010000000
VERSION     DASUPW010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1755109"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..532
                     /locus_tag="VFY44_08825"
     CDS             137..532
                     /locus_tag="VFY44_08825"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX5910583.1"
                     /translation="MKTHTVEGQFLLDRVGGLLGRIGEVVRSCHERWDGKGYPDGLKG
                     EQIPLAARIVFVCDAYNAMTTDRPYRSAMSTQDALDELQANAGTQFDPTIVAALVEVV
                     QDAQPESRAADDMRMLLSEVRLSDAVTTS"
     gene            complement(572..1750)
                     /locus_tag="VFY44_08830"
                     /pseudo
     CDS             complement(572..1750)
                     /locus_tag="VFY44_08830"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(2049..>2973)
                     /locus_tag="VFY44_08835"
     CDS             complement(2049..>2973)
                     /locus_tag="VFY44_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX5910584.1"
                     /translation="VGLVGESGCGKTTLARTLMRLLEPSAGQIRFDGQDITTANRKSL
                     QPLRRDMQMVFQDPFASLNPRKTVRQLVSAPLLLHGMGREEAEGKVDELLERVGLAPE
                     HARRFPHEFSGGQRQRIGIARALALQPRMIVLDEPVSALDVSVQAQIVNLLDDLQEEF
                     GLAYLFVAHDLSVVRHVSDRIAVMYLGKLKELSPSEALYSKPIHPYTHALLSAVPIPD
                     PRENRARKRALVTGEPPNPIDPPSGCVFHPRCPRATDVCKTVEPPLTRYADGHLAACH
                     HPLNVTEAEIASAERSDASPESAGAELPSAA"
BASE COUNT          494 a         1044 c         1067 g          368 t
ORIGIN      
        1 tggcttgcac caaatatatc ggcggccatg ccgacctgat gctcggctcg gtcaccacca
       61 ccgaacatta tgccgagcgt ctcgagcaga gccggcgggt gctcggccag accgcgagtc
      121 cggacgacgc ctggctttga agacccacac cgtcgagggc cagttcctgc tggaccgcgt
      181 cggcggcctg ctgggacgaa tcggcgaggt cgtccgctcg tgccacgagc gctgggacgg
      241 aaagggctac ccggacggcc tgaagggcga gcagatcccg ctggccgccc ggatcgtctt
      301 cgtctgcgac gcctacaacg ccatgaccac cgaccggccc taccgcagcg cgatgagcac
      361 ccaggacgcg ctggacgagc tacaggccaa cgccgggacg cagttcgacc ccacgatcgt
      421 cgcggccctg gtcgaggtcg tccaggatgc ccagccagag agccgtgccg ccgacgacat
      481 gcggatgctg ctctccgaag tgcggctcag cgacgccgtc acgaccagct gaggacagcg
      541 ccgcgggccg gtcccgccag tgcccgcgca ggacgacgac cgccagcagc gccagcgaga
      601 cctcccacgc gccggtgatc gccagcagct ggcgcggatc gaacacggtc atcagcgcgc
      661 cgccggccag cagcgcgatg accatcgccc aggacaccag cgtgtccgtc agcgcgaacg
      721 tccgcccgcg gaagcgctcc gggacctcgg actggatcag cagccgctgg taggtcacga
      781 acagcccgtt gccgaagccg cccagggcga acgccagcag ggcggtgtac aggctcggcg
      841 acagggcggt gagcagcccg gccacgccgg tcagcaggat gccctgcacg aagcggcgct
      901 tgagcagggg ggcggtgccc cccgacgcgc cggtcagcga tcccgccagg aagcccaggc
      961 ccagggccgc caccagcgcc gagtagccgg agccgccggc gtccagcgtg tcctcggcga
     1021 agggcagctc gacgacgttg aacacgccgc ccgagagcat cgccccgccc atcaggccga
     1081 ccaggatcgc cacgggccgc atgcgcatga cggcctggat gcccgaacgg gtgtcgccca
     1141 gcagcgtctc gcgctcggcg tcctgctcgc gcgccgggcg gggaccgaag tccagccgcc
     1201 ccaggaccag cgcggagatc aggaaggtcg ccccgttggc caccagcaac ccctcggggc
     1261 tggccagggc cagcaccccc gcggccagcg ccggcccgag cgtgtagccc acgtcggtca
     1321 cggccccgta cgcggacgtg gccgccgggg cccggtcggg gctgaccagg ccggggatgg
     1381 ccgacagcat cgcggggcgg aacagcgcgg tgccggtgcc tgccagcagc gcgacgatca
     1441 gggtgatctc gaagctgccg acgaagacga ccgtcacgaa cgcggcggcg cggaccacgt
     1501 ccgcgaccac cacgcaccag cggcgcgacc agcgatcggc cgccgcgccc gcaagcggcc
     1561 cgagcaccat cgtgggcagg aactcggcga gcaggatgag gctgatggcc cacggggagc
     1621 gccagcggtc gtaggcgacc aggaggaggg cgacgtagcc ggcgcccgtg ccgagcgcgg
     1681 actgcgccac ggcggccaac atgatgcggg cacgtacctc gtgccgcagg agctgaaggg
     1741 tctcacgcac cggatcgaag cctacgctcg cctgctgacc ggaagaggag cgcggtgggt
     1801 cgcgctcgag ctgagcgcga ccccacgacg catggaacta ctagtgacgc ctcggcatag
     1861 tggctccctt cgaagctggt accggtctca aactccactc gctctgcaca gataagtggg
     1921 gcaaacccgt gcagttcgca tcactttgtc acaagatccg tgcttgcgcc aactttttgc
     1981 aagaacctgt gcaaagggga ggagaacccc tatcgggtca ctagcggcgc ttctgaacgg
     2041 aagcggctct aggccgcgga gggcagctcg gcgccggcgg attccgggct ggcgtccgag
     2101 cgctcggcgg aggcgatctc ggcctccgtc acgttcagcg ggtggtggca ggcggccagg
     2161 tggccgtccg cgtaccgggt cagcgggggc tcgacggtct tgcagacgtc ggtggcacgg
     2221 gggcagcggg ggtggaacac acagcccgag ggcggatcga tcgggttggg cggctcgccc
     2281 gtgaccagcg cacgcttgcg cgcccggttc tcgcgcgggt ccgggatcgg gacggcgctg
     2341 agcagggcgt gcgtgtacgg gtggatcggc ttcgagtaca gggcctccga cggcgagagc
     2401 tccttgagct tgcccaggta catgacggcg atccggtcgg agacgtggcg cacgacgctg
     2461 aggtcgtggg ccacgaacag gtaggccagg ccgaactcct cctggaggtc gtccaggagg
     2521 ttcacgatct gggcctggac cgacacgtcc agggccgaga ccggctcgtc gagcacgatc
     2581 atgcgcggct gcaacgccag cgcacgggcg atgccgatgc gctggcgctg gccacccgag
     2641 aactcgtgcg ggaaccggcg cgcgtgctcg ggcgccaggc cgacgcgctc gagcagctcg
     2701 tcgaccttgc cctcggcctc ctcacgcccc atgccgtgca ggagcagcgg cgcggacacc
     2761 agctggcgca cggtcttgcg cgggttcagc gaggcgaacg ggtcctggaa caccatctgc
     2821 atgtcccggc gcagcggctg gagggacttc cggttcgcgg tggtgatgtc ctggccgtcg
     2881 aagcggatct ggcctgcgct gggctcgagc agtcgcatca gggtccgcgc cagcgtcgtc
     2941 ttgccacagc ccgactcccc caccaggccc acc
//
LOCUS       DASUPW010000395         2766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_509436, whole genome shotgun sequence.
ACCESSION   DASUPW010000395 DASUPW010000000
VERSION     DASUPW010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509436"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VFY44_08840"
     CDS             complement(<1..199)
                     /locus_tag="VFY44_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910585.1"
                     /translation="MASPVLVGAVTLLVVIVGVFLAYNANSGLPFVPTYTLEAELPSG
                     AKLVKGNEVRVGGFRVGAVSEI"
     gene            complement(226..1812)
                     /locus_tag="VFY44_08845"
     CDS             complement(226..1812)
                     /locus_tag="VFY44_08845"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5910586.1"
                     /translation="MRRLATAAAMVVALALGAVALTGADGGGGQTREVRMVFDNAFGL
                     VEGGDFRIGGVRAGQTTGFDVQESRSGPPKAIVKATIDRSGAAAELRRDATCTIRPQS
                     LVGEYYVDCQPGSARAKLGEGDVIPLRRTEGTIPQDLVNDVLRRPTRERLRLFVASLG
                     TGLAGRPEDIQAVLRRSFPGLRETRKTLRILGDQNRTIKQFIADADTVVTRLHERRGD
                     VTDFIRESRGAAETGASRRAELREGVRLLPGFLTELRPTMAELSNLARQGTPLAGDLQ
                     RSAPAATTLLRRLGPFAEAARPALRGLGEAAVPATQGLRESGQELASLRALARQAPGT
                     VKPLRQTLQSLDDRRRAVDQDPRAAQTAPPAPDPTSNASGRGFTGFESLVNYFFWQTM
                     TTNGFDAIGHQLRVGATVSKCSPYRNEPPRNAADEQLFRDCNSWLGPNQPGINAPDPS
                     VPGSAASKARAAKGDAPSTPKVELPPALKKLVERTGGNTKPIDPTAPAAPGTGAPTPA
                     GGTPQAQPSPTGLLDFLLKP"
     gene            complement(1809..>2766)
                     /locus_tag="VFY44_08850"
     CDS             complement(1809..>2766)
                     /locus_tag="VFY44_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910587.1"
                     /translation="DEVFNTLDAPTRRSLEQLLTRGAESVNGRGEDVGRALEYLDPAL
                     STTSRLFREATRDTPNLRASLRNSAQLVSTLSERDTQLAALVGDLSTTTRALGSQKGA
                     LAESVGLLPPVLRRANTSFVNLRLALDDVDPLVNATKPLLPKLRPTFAATRQLAADSR
                     PALADLRAALSRRGANNDLLELMRDIPPLADVAVATKDRTVAPGGRNVSVGRTTGAFP
                     QTAAGLKAATPVIGFGRPYTTDFLGWLDDFSTTGGFYDALGASTRVFISVSENTTGGP
                     PKREQFRRCPGGADVPARDGSNVLSEAEQRELNCTESHRAIR"
BASE COUNT          316 a          994 c         1074 g          382 t
ORIGIN      
        1 cgatctccga gaccgccccc acccggaacc cgccgacgcg gacctcgttg cccttcacca
       61 gcttggctcc gctgggcagc tcggcctcga gcgtgtaggt gggcacgaac gggagcccgg
      121 agttggcgtt gtaggccagg aacacgccga cgatgaccac cagcagcgtc acggcgccca
      181 ccagcacggg gctggccacg atggtcgggg atgtgcggtt gcggttcacg gcttcagcag
      241 gaagtcgagg agcccggtcg ggctgggctg ggcctgcggc gtaccgcccg ccggcgtggg
      301 cgcgccggtc ccgggggcgg cgggcgcggt cgggtcgatc ggcttggtgt tgccgccggt
      361 gcgctcgacc agcttcttca gcgcgggcgg cagctcgacc ttgggggtcg agggcgcgtc
      421 gcccttggcg gcgcgggcct tcgacgcggc cgagcccggc accgacgggt caggcgcgtt
      481 gatgcccggc tggttcgggc ccagccacga gttgcagtcg cggaagagct gctcgtcggc
      541 ggcgttgcgc ggcggctcgt tgcggtaggg gctgcacttc gacaccgtcg cgcccacccg
      601 cagctggtgg ccgatggcgt cgaagccgtt ggtggtcatc gtctgccaga agaagtagtt
      661 gaccagcgac tcgaagccgg tgaagccccg gccggaggcg ttggacgtgg ggtccggggc
      721 ggggggcgcg gtctgcgccg cacgcgggtc ctggtccacc gcgcggcggc ggtcgtccag
      781 gctctgcagg gtctggcgca gcggcttgac cgtgccgggc gcctggcgcg cgagcgcgcg
      841 cagcgaggcc agctcctgac cgctctcacg caggccctgg gtggccggga cggccgcctc
      901 tcccagcccg cgcagcgcgg gccgggcggc ctcggcgaac gggcccaggc gccggagcag
      961 ggtggtggcc gcgggggccg accgctggag gtcgccggcc agcggtgtgc cctgcctggc
     1021 caggttcgac agctccgcca tggtcggccg cagctcggtc aggaagccgg gcagcaggcg
     1081 cacgccctcg cgcagctcgg cccggcgcga cgcgccggtc tcggccgcgc cgcgcgactc
     1141 gcggatgaag tcggtcacgt caccacggcg ctcgtgcagg cgcgtgacca cggtgtccgc
     1201 gtcggcgatg aactgcttga tcgtgcggtt ctggtcgccc aggatccgga gcgtcttgcg
     1261 cgtctcccgc agcccgggga acgagcgccg gagcaccgcc tggatgtcct cggggcggcc
     1321 ggccaggccg gtgccgagcg aggccacgaa caggcgcagt cgctcgcgcg tcgggcggcg
     1381 cagcacgtcg ttgaccaggt cctgggggat cgtgccctcg gtccgccgca gcgggatcac
     1441 gtcgccctcg ccgagcttcg cccgtgccga ccccggctgg cagtccacgt agtactcgcc
     1501 caccagcgac tgcggccgga tcgtgcaggt ggcgtcccgg cgcagctcgg ccgcggcgcc
     1561 ggagcggtcg atggtggcct tgacgatcgc tttcggcggc ccgctccgcg actcctggac
     1621 gtcgaagccc gtggtctgcc cggcgcgcac gccgccgatg cggaagtcgc cgccctccac
     1681 caggccgaag gcgttgtcga agaccatgcg cacctcgcgc gtctgtcccc cgccgccgtc
     1741 ggcgccggtc agcgccaccg cgcccagcgc cagggccacc accatggccg cggctgtggc
     1801 caggcgcctc accggatcgc cctgtgggac tcggtgcagt tcagctcgcg ctgctcggcc
     1861 tcgctcagca cgttcgaccc gtcacgggcg ggcacgtccg cgccgccggg gcagcgccgg
     1921 aactgctccc gcttgggcgg gccgccggtg gtgttctcgg acaccgagat gaacacgcgc
     1981 gtgctggcgc cgagcgcgtc gtagaagccg ccggtggtgg agaagtcgtc cagccagccc
     2041 aggaagtcgg tggtgtacgg gcggccgaag ccgatcaccg gcgtggcggc cttcagcccg
     2101 gcggccgtct gcgggaaggc gccggtcgtg cggcccacgc tgacgttgcg cccgccgggg
     2161 gcgacggtgc ggtccttcgt ggccaccgcg acgtcggcca gcggcgggat gtcgcgcatc
     2221 agctccagca ggtcgttgtt cgcgccgcgg cggctgagcg cggcgcgcag gtcggccagc
     2281 gcggggcgcg agtcggcggc cagctgccgg gtggcggcga aggtgggccg cagcttgggc
     2341 aggagcggct tggtggcgtt caccagcggg tcgacgtcgt ccagcgccag gcgcaggttc
     2401 acgaacgacg tgttggcgcg acgcagcacc ggcggcagca ggcccaccga ctcggccagc
     2461 gcacccttct ggctgcccag cgcgcgggtc gtggtgctca ggtctccgac cagcgcggcc
     2521 agctgcgtgt cgcgctcgct cagcgtggac accagctgcg cgctgttgcg caggctggcc
     2581 cgcaggttcg gcgtgtcgcg cgttgcctcg cggaacagcc ggctcgtggt cgacagcgcc
     2641 gggtccaggt actccagcgc ccgccccacg tcttcgccgc ggccgttcac cgactccgcg
     2701 ccccgggtca ggagctgctc caggctccgg cgggtgggcg cgtcgagcgt gttgaacacc
     2761 tcgtcg
//
LOCUS       DASUPW010000396         2060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_923900, whole genome shotgun sequence.
ACCESSION   DASUPW010000396 DASUPW010000000
VERSION     DASUPW010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923900"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VFY44_08855"
     CDS             complement(<1..273)
                     /locus_tag="VFY44_08855"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX5910588.1"
                     /translation="MPELPEVEIAARRVGAAVAGARVESALAPGMVTMKTFDPPLDTL
                     VGREIDGVRRRGKMFVVELGDLALLIHLMSAGRLQVWDKRASLRDRA"
     gene            306..734
                     /locus_tag="VFY44_08860"
     CDS             306..734
                     /locus_tag="VFY44_08860"
                     /inference="COORDINATES: protein motif:HMM:NF014552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEX5910589.1"
                     /translation="MSVSSDERTGVADALVERLRALGHEPLLHGALSGDERDDWAWAS
                     EAAARDVADGRAHQGIVCCWTGTGASIAANKVGGVRAALCADAETASGARRWNDANVL
                     ALSLRTTSEALLEEILEAWFAGEPSTEDDDAANIAHVDQI"
     gene            complement(731..1306)
                     /locus_tag="VFY44_08865"
     CDS             complement(731..1306)
                     /locus_tag="VFY44_08865"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX5910590.1"
                     /translation="MAITCLLGVAAPAQAKTVNAGIGDFFFRADRVRVDPGDTVAWTN
                     RGATLHNVTSRRGAPEPFRSSDLDSGQAFSRVFVKPGTYDYLCDIHPDQMRGTVQVGP
                     DRTKPKVTGVRATAGRRVRVSYRVSERSRVTVRLIRRGRTVKSVRTRKPVSGSSAQRL
                     ALPAAGAYRVSVTATDLEGNTGTARTSLTVD"
     gene            1357..1824
                     /locus_tag="VFY44_08870"
     CDS             1357..1824
                     /locus_tag="VFY44_08870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX5910591.1"
                     /translation="MRVSAKADYAVRAAVELAVAREGDPPRPVKGDHVAKAQAIPIKF
                     LENILLDLRQGGLVNSRRGPDGGYWLSRPPEEITVADVIRAADGPLASVRGEKPEDLR
                     YEGSAQPLATVWVALRSSLREVLEQVTLADLAADKLPAHVQKRSQEADAWQRR"
     gene            complement(1821..>2060)
                     /locus_tag="VFY44_08875"
     CDS             complement(1821..>2060)
                     /locus_tag="VFY44_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910592.1"
                     /translation="KRCTRRALKVRIRGADADLVIRSRFALRSRRGRDAAEPFTFTVR
                     RKALRAGRRYRLKASARLYDGRVGRVSVRVRGCRG"
BASE COUNT          281 a          762 c          768 g          249 t
ORIGIN      
        1 ggcgcggtcg cgcagcgagg cgcgcttgtc ccacacctgc agccggccgg cggacatcag
       61 gtggatcagc agcgccaggt cgcccagctc gaccacgaac atcttgcccc gccggcgcac
      121 gccgtcgatc tcccgcccca ccagcgtgtc cagcgggggg tcgaacgtct tcatggtcac
      181 catgccgggc gccagcgcgg actccacgcg cgcgccggcc acggcggcgc cgacgcggcg
      241 ggcggcgatc tcgacctcgg gtagctccgg catgggctac gaggctaccc tcggcggcga
      301 tgcgggtgag cgtgtcatcg gacgagcgca cgggcgtcgc ggacgccctg gtcgagcgcc
      361 tgcgcgcact gggccacgag ccgctgctgc acggcgcgct gtccggcgac gagcgcgacg
      421 actgggcctg ggccagcgag gcggcggccc gcgacgtggc cgacgggcgc gcccaccagg
      481 gcatcgtctg ctgctggacc gggacgggcg cgtcgatcgc cgcgaacaag gtcggcggcg
      541 tgcgcgcggc cctgtgcgcc gacgccgaga ccgcgtccgg ggcgcgccgg tggaacgacg
      601 cgaacgtgct ggcgctgtcg ctgcgcacca ccagcgaggc gttgctggag gagatcctcg
      661 aggcctggtt cgccggcgag cccagcaccg aggacgacga cgcggccaac atcgcccacg
      721 tcgaccagat ctagtccacg gtcagcgacg tgcgggcggt gcccgtgttg ccctcgaggt
      781 cggtggccgt gaccgacacc cggtaggcgc ccgccgccgg gagcgcgagc ctctgggcgg
      841 aggagccgct gaccggcttc ctcgtgcgca ccgacttcac cgtcctgccc cgccggatca
      901 gccgcaccgt cacccgcgag cgctcggaca cgcggtacga gacccgcacc cgcctgccgg
      961 ccgtggccct gaccccggtg accttcggct tcgtgcgatc cgggcccacc tgcacggtcc
     1021 cgcgcatctg gtcgggatgg atgtcgcaga gatagtcgta ggtgccgggc ttgacaaaca
     1081 cccggctgaa ggcctgtccc gagtccaggt cgctcgagcg gaagggctcg ggcgcgccgc
     1141 ggcggctggt cacgttgtgc agggtggccc cgcggttggt ccacgcgacg gtgtcgcccg
     1201 ggtcgacccg gacccggtcg gcacggaaga agaagtcgcc gatcccggcg ttcaccgtct
     1261 tcgcctgtgc cggcgcggcc actccgagca ggcacgtgat tgccaccgcg gagaccctcc
     1321 acatgcctcc gagtctagat cgtggagaat ggcggcgtga gagtctcggc caaggcggat
     1381 tacgccgtgc gcgccgccgt ggagctggcg gtcgcgcgcg agggcgaccc gccccgcccg
     1441 gtcaagggcg accacgtggc caaggcccag gccatcccga tcaagttcct ggagaacatc
     1501 ctcctggacc tgcgccaggg gggcctggtg aacagccgcc gcgggcccga cggcggctac
     1561 tggctgtcca ggccgccgga ggagatcacg gtggccgacg tgatccgcgc ggcggacggg
     1621 ccgctggcca gcgtccgggg cgagaagccc gaggacctgc gctacgaggg ctcggcccag
     1681 ccgctggcca ccgtgtgggt cgccctgcgc tcgagcctgc gcgaggtgct ggagcaggtc
     1741 accctggccg acctggccgc cgacaagctg ccggcgcacg tgcagaagcg cagccaggaa
     1801 gccgacgcct ggcagcgccg ctagccgcgg cagccgcgca cgcgcaccga cacccgcccg
     1861 acgcgcccgt cgtacaggcg cgcgctggcc ttgaggcggt agcgccggcc ggcgcgcagc
     1921 gccttgcggc ggacggtgaa ggtgaacggc tccgcggcat cgcggccgcg ccgcgagcgc
     1981 agtgcgaacc ggctccggat cacgaggtcg gcgtcggcgc cccggatgcg caccttcagc
     2041 gcccgccgcg tgcatcgctt
//
LOCUS       DASUPW010000397         2611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1202096, whole genome shotgun sequence.
ACCESSION   DASUPW010000397 DASUPW010000000
VERSION     DASUPW010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202096"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..814)
                     /locus_tag="VFY44_08880"
     CDS             complement(50..814)
                     /locus_tag="VFY44_08880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEX5910593.1"
                     /translation="MRTREILGIPMAMTDYDGAMDVMDDMVEKRRRGWVCAAAVHSVM
                     VAQDDPGMRAALTGAEITVPDGMPVVWAANMMGENLPNRVYGPELMQRYCARGVERGH
                     RVWLYGGRDQGALVQLALTLRHSHPGIKIVGGYSPPFRALTEDEDDRIAEQINRDKPD
                     VIWVGIGVPKQEKWMVRMRDKLEAPVMCAVGAAFDFNAGRVSQAPRWMQERGLEWTYR
                     IAQEPRRLLPRYVYTNPRFMTGVARQYLRERSSRPS"
     gene            1013..2548
                     /locus_tag="VFY44_08885"
     CDS             1013..2548
                     /locus_tag="VFY44_08885"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX5910594.1"
                     /translation="MPDSHRPVHEPPESPDGGQARRVAAGALTQQIAQVTGLVVLLAV
                     VTVLARRLSLAELGTYGLTATLATYLLIVKNSIGGAAQRAMVAARGPEDQSAAFSTAA
                     GLYALTGLVTGLLIAGVGAGLAAAILDGDLREQAQLGVLGLGAVSAVGLAATVNLDAM
                     RAALLLTRSAANEIAALVVFAALMLTLALSDAPLWALIAASGTIPLLSGTVNLVARRR
                     LGLPFRLRAGGFQRERARALAPTAGWLLLIEVSTLVIYGLDRVVLGLFGSPASIGLYE
                     GPVRAHNVIYALNQSVGVTTLPVGASLTAQGDRQRLRELVLRGSRYTLALFVPLTVTA
                     MAMAAPALEVWLGERFREAAPALTILVSYWLLWGMLAVTPNFLVGAGRARQAGSVVAG
                     IAALNLALSLALTPSLGVEGPALGTAIAYVAGFPLMLRIGLDVGGLSLAELARGAWLP
                     AYALGAVLAALLVGARLALDPDTLVVVLPMVLAGPLAYGAAYYGLVMDASERAFVRSL
                     LRR"
BASE COUNT          295 a          924 c         1000 g          392 t
ORIGIN      
        1 ggcgacagtc cggcgccgca ggcggggcag ggtgccacgc cgcggcagtc taggacgggc
       61 ggctggagcg ttcccggagg tactggcgcg cgacgccggt catgaaccgc gggttggtgt
      121 agacgtagcg cggcagcagc cggcgcggct cctgggcgat ccggtaggtc cactccaggc
      181 cgcgctcctg catccagcgc ggggcctgcg acacgcggcc ggcgttgaag tcgaacgcgg
      241 cgccgacggc gcacatgacc ggggcctcga gcttgtcgcg catgcgcacc atccacttct
      301 cctgcttggg cacgccgatg ccgacccaga tcacgtccgg cttgtcgcgg ttgatctgct
      361 cggcgatgcg gtcgtcctcg tcctcggtca gggcgcggaa ggggggcgag tagccgccga
      421 cgatcttgat cccggggtgg ctgtggcgca gcgtcagggc cagctggacc agcgcgccct
      481 ggtcgcggcc gccgtacagc cacacgcggt ggccgcgctc gacgccacgg gcgcagtagc
      541 gctgcatcag ctcggggccg tagacgcggt tgggcaggtt ctcgcccatc atgttggccg
      601 cccacaccac gggcatgccg tccgggacgg tgatctccgc gccggtcagc gcggcccgca
      661 tgcccgggtc gtcctgggcg accatgaccg agtgcacggc ggcggcgcag acccagcccc
      721 gccggcgctt ctcgaccatg tcgtccatga cgtccatggc gccgtcgtag tcggtcatcg
      781 ccatcgggat gccgaggatc tcgcgggtgc gcacgcctgg cagggcggac ggagcgagag
      841 attcggagat gggagtgggt gcgggtttca tgggagaagc ggccggcgat ctcgataacc
      901 cctccctggt gcgcccccga ccaataagtg agggtaccca caggacctaa tcgtcaacct
      961 cccctgcagg aacccggcca cgacgtcgat gattcggtgg gttccggcgg acgtgccaga
     1021 ctcgcaccgc cccgtgcacg agcctccgga atctcccgac ggcgggcagg cccggcgcgt
     1081 cgccgcgggc gccctgaccc agcagatcgc ccaggtcacc gggttggtgg tcctgctggc
     1141 ggtcgtgacc gtgctcgcgc ggcggctgtc gctggccgag ctgggcacgt acgggctgac
     1201 ggccacgctg gccacctacc tgctgatcgt gaagaactcc attggcgggg cggcccagcg
     1261 ggcgatggtg gccgcgcgcg ggcccgagga ccagtcggcc gccttctcca cggcggccgg
     1321 gctgtacgcg ctgaccggcc tggtcaccgg gctgctgatc gcgggcgtgg gggcggggct
     1381 ggccgccgcc atcctggacg gcgacctgcg cgagcaggcc cagctcggcg tgctgggcct
     1441 gggcgcggtc agcgccgtgg gcctggccgc caccgtgaac ctggacgcca tgcgcgcggc
     1501 gctgctgctc acccgcagcg ccgccaacga gatcgccgcg ctggtggtct tcgccgccct
     1561 gatgctgacc ctggcgctgt cggacgcgcc gctctgggcc ctgatcgcgg ccagcggcac
     1621 gatcccgctg ctgtcgggga ccgtgaacct ggtggcgcgg cgtcgcctgg ggctgccctt
     1681 ccggctccgt gccggcggct tccagcgcga gcgggcccgg gcgctggcgc ccacggccgg
     1741 ctggctgctg ctgatcgagg tctcgacgct ggtcatctac ggcctggacc gggtggtgct
     1801 gggcctgttc ggatcgcccg cctcgatcgg cctgtacgag ggccccgtgc gcgcccacaa
     1861 cgtgatctac gcgctcaacc agtcggtggg cgtgaccacg ctcccggtgg gcgccagcct
     1921 gaccgcccag ggggaccgcc agcgcctgcg cgagctcgtg ctgcgcgggt cgcgctacac
     1981 gctggcgctg ttcgtgccgc tgaccgtgac cgccatggcc atggcggcgc ccgcgctcga
     2041 ggtgtggctc ggcgagcgct tccgcgaggc cgcgccggcg ctgaccatcc tggtctccta
     2101 ctggctgctg tgggggatgc tggccgtgac cccgaacttc ctggtcggcg cgggccgcgc
     2161 gcgccaggcc gggtcggtgg tggccggaat cgccgcgctg aacctggccc tgtcgctggc
     2221 gctgacgccc tcgctgggcg tggaggggcc ggcgctgggc acggccatcg cctacgtggc
     2281 cgggttcccg ctgatgctgc ggatcggcct ggacgtgggc gggctgtcgc tggccgagct
     2341 ggcgcggggc gcctggctgc cggcctacgc cctcggcgcc gtgctggccg cgctgctggt
     2401 gggcgcgcgg ctggcgctgg acccggacac gctggtggtg gtgctcccga tggtgctggc
     2461 cgggccgctg gcctacgggg cggcctacta cggcctggtc atggacgcgt ccgagcgcgc
     2521 gttcgtacgc tcgcttctca gacgctgaac ggcagggcgg cggcgatctc ggacaggcgc
     2581 ggcgcggacg cgtcgcgctc ggagtagacc a
//
LOCUS       DASUPW010000398         2445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1202182, whole genome shotgun sequence.
ACCESSION   DASUPW010000398 DASUPW010000000
VERSION     DASUPW010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202182"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VFY44_08890"
     CDS             <1..387
                     /locus_tag="VFY44_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910595.1"
                     /translation="GSALGTQGTQNVARVVVATWERARDLRTVRALEQGPRRSAVFAR
                     FSPAGDLTLLDEMARPVRDAGRDAGLVAALRPTEESLIWVVSGGSRKGMDRAARAFRR
                     TALRDAFAVAAPASGPTVPLPVGAGR"
     gene            384..1274
                     /locus_tag="VFY44_08895"
     CDS             384..1274
                     /locus_tag="VFY44_08895"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEX5910596.1"
                     /translation="MKPGFVPVYQPRPSPLHTAGAGATSALCGAFALVCLLFPHPLLL
                     TAALAAIVGCAAAAGVGGQLVRALRITLPLGLLVALINPLVYNEGATVLIRGGTLFGH
                     RFDITLEATMAGLIQGYRVVVFGMAFALFSACVDQDDVLRALRRFSYRSALTAVLATR
                     LVPVLARDASRMSDASRCRPVPAGRLEVARAALAGSLERAVDVAAALEVRGYSNAARP
                     ARRRRRRSRHDVRVLTAAALVAAGAVALRVAGAGWVQEYPTLDMPLGAAELAVCVVVL
                     AGAALPFAGRGARMGVAARA"
     gene            1267..>2445
                     /locus_tag="VFY44_08900"
     CDS             1267..>2445
                     /locus_tag="VFY44_08900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5910597.1"
                     /translation="MPDVLARAEGFGYRYAGRDVDALTGIDLELDEGSFTVLAGLSGS
                     GKSTLLRALCGLVPHFHGGHARGTLEVGGMDLREHGPADLASVCGTVFQDPESQVVMG
                     GVRAEIALALEHRGQDEAAIARAVEEVALTMGIGGLLDRRTDSLSGGELQRVAIASAL
                     VARPRLLLLDEPTSQLDPVAGDELVWMLRRINEEWGTAVVLAEHRLERCLPAADRVIA
                     MIDGEVACDAPPGEFLEWASSAHPALATPAARLFSLAGLRPLPASVKDAHAGLREAGV
                     TPRGDAKPEEAGLPGRPRWWRRRRGDEPEPALALREVWHEVEDGPTILRGIDLVLRPG
                     ERVALMGRNGAGKSTMLRIARGLAEPTRGRVERAGEVALLLQNPGDYLVHDHAVEDAG
                     A"
BASE COUNT          239 a          905 c          984 g          317 t
ORIGIN      
        1 ggctcggccc tgggcaccca ggggacccag aacgtcgcgc gggtggtggt ggccacctgg
       61 gagcgcgcgc gtgacctgcg caccgtccgc gccctggagc agggcccgcg gcgcagcgcc
      121 gtgttcgccc gcttcagccc cgccggcgac ctgacgctgc tggacgagat ggcccgcccg
      181 gtgcgcgacg ccggccgcga cgccgggctg gtcgcggcgc tgcgccccac cgaggagtcg
      241 ttgatctggg tggtctccgg cggatcgcgg aagggcatgg accgggccgc ccgcgcgttc
      301 cggcggaccg cgctgcgcga cgccttcgcc gtcgccgcgc cggcgtcggg ccccaccgtc
      361 ccgctgcccg tgggggccgg gcggtgaagc ccgggttcgt cccggtctac cagccgcggc
      421 cgagcccgct gcacaccgcc ggcgccggcg ccacctcggc cctgtgcggc gccttcgcac
      481 tggtctgcct gctgttcccg cacccgctgc tcctgaccgc ggcgctggcg gcgatcgtcg
      541 gctgcgccgc cgcggccggg gtgggcgggc agctggtgcg cgcgctgcgc atcacgctgc
      601 cgctgggcct gctggtcgcg ctgatcaacc cgctggtcta caacgagggc gcgaccgtgc
      661 tgatccgcgg cggcaccctg ttcggccacc gcttcgacat cacgctcgag gccaccatgg
      721 cgggcctgat ccagggctac cgggtggtcg tgttcgggat ggccttcgcg ctgttctcgg
      781 cctgcgtgga ccaggacgac gtgctccggg ccctgcggcg cttctcctac cggtcggcgc
      841 tgacggcggt gctggccaca cgcctggtgc cggtcctggc ccgcgacgcc tcgcggatga
      901 gcgacgcctc gcgctgccgg cccgtgcccg ccgggcggct ggaggtggcc cgggcggcgc
      961 tggcgggctc gctggagcgg gcggtcgacg tggccgccgc gctcgaggtg cgcgggtact
     1021 cgaacgccgc ccggcccgcg cgccggcggc gccggcgctc gcggcacgac gtgcgcgtgc
     1081 tgaccgcggc ggcgctcgtg gccgccggcg cggtggcgct acgggtcgcg ggggccggct
     1141 gggtgcagga gtacccgacg ctggacatgc cgctgggagc ggccgagctg gccgtgtgcg
     1201 tggtggtgct ggcgggggcc gcgctgccgt tcgccggccg tggcgcccgg atgggggtgg
     1261 ccgcccgtgc ctgacgtgct ggcccgtgcc gagggcttcg gataccgcta cgccggccgc
     1321 gacgtggacg ccctgaccgg catcgacctg gagctcgacg aaggcagctt cacggtcctg
     1381 gcggggctgt cgggctcggg caagtcgacg ctgctgcggg cgctgtgcgg gctggtgccc
     1441 cacttccacg gcgggcacgc gcgcgggacg ctggaggtcg gcggcatgga cctgcgcgag
     1501 cacgggccgg cggacctggc cagcgtgtgc gggaccgtgt tccaggaccc cgagtcgcag
     1561 gtggtcatgg gcggcgtccg cgcggagatc gcgctggcgc tggagcaccg cggccaggac
     1621 gaggcggcca tcgcgcgcgc cgtcgaggag gtggccctga ccatgggcat cggcgggctg
     1681 ctggaccggc gcaccgactc gctctcgggc ggggagctgc agcgcgtggc catcgcctcg
     1741 gcgctggtgg cccgtccccg gctgctcctg ctggacgagc ccacgtcgca gctggacccc
     1801 gtggccggcg acgagctggt gtggatgctg cgccggatca acgaggagtg ggggaccgcg
     1861 gtggtgctgg ccgagcaccg cctggagcgc tgcctcccgg ccgccgatcg cgtgatcgcg
     1921 atgatcgacg gcgaggtggc ctgcgacgcc ccgcccggcg agttcctcga gtgggcatcg
     1981 agcgcgcatc cggcgctcgc aacgcccgct gcgcgcctgt tctcgctcgc cggcctgagg
     2041 ccgctgccgg cctccgtgaa ggacgcgcac gcgggcctgc gcgaggcggg cgtcacgccg
     2101 cgcggggacg cgaagcccga ggaggccggc ttgcccgggc ggccgcgctg gtggcgccgc
     2161 cgccgcggcg acgagcccga gcccgccctc gccctccgcg aggtctggca cgaggtcgag
     2221 gacgggccca cgatcctgcg cggcatcgac ctcgtcctcc ggcccggcga gcgcgtcgcg
     2281 ctcatgggcc ggaacggcgc cggcaagagc accatgctgc gtatcgcgag gggtctcgcc
     2341 gaaccgaccc gcgggcgcgt cgagcgagcg ggggaggtgg cgctgctgct ccagaacccc
     2401 ggcgactacc tggtgcacga ccacgccgtc gaggacgccg gcgca
//
LOCUS       DASUPW010000399         2839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1202718, whole genome shotgun sequence.
ACCESSION   DASUPW010000399 DASUPW010000000
VERSION     DASUPW010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202718"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="VFY44_08905"
     CDS             complement(<1..957)
                     /locus_tag="VFY44_08905"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5910598.1"
                     /translation="MQRKWQVLLVTAVAVFMGFLDVSIVNVAFPDIERSFPGTSEAGL
                     SWVLNAYNVVFAALLVPAGRAADLVGRRKTFFVGMGVFLLASALCAAAPSVELLVAAR
                     VLQAVGAAILIPTSLGLLLPEFPPEQRATATSIWGATGAVAAATGPSLGGVLVDAANW
                     RWVFLINVPIGLAALIPARRILREYRDESRGAVPDLLGAALLMGGVGLIALAIVQGES
                     WGYGSARIVGSGAVGIALLAAMVLRSRTHTAPVLDLDLFRTRSFAVAVTGLMVFSVGF
                     YALLLGNILFLTRVWDYSIVTAGFAVTPGPLMAAVSSVVGGRL"
     gene            1020..1478
                     /locus_tag="VFY44_08910"
     CDS             1020..1478
                     /locus_tag="VFY44_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5910599.1"
                     /translation="MLPRDYDTQNCSVARTLEAVGDRWTMLVIREAFLRTRRFEDFQA
                     HLGIARNVLSDRLSRLVDDGILDRRPYQDSPPRFEYRLTEKGLDLWPVLMTLMAWGDR
                     HAAEHGPPRLIVHRDCGGTLDRHLRCERCGQDLGPRDVEAQPGPGAVALQ"
     gene            1511..>2839
                     /locus_tag="VFY44_08915"
     CDS             1511..>2839
                     /locus_tag="VFY44_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5910600.1"
                     /translation="MPLFAFTFQEFQDWAVIWLPILFMALIAGTLLVLLRHMPRTKPQ
                     EIKPSSQDSISWDDVAGVEEAKDELREVVDFLRDPDRFKQLGAQVPKGILLHGPPGTG
                     KTLLAKAVAHESNAQFFAQSAASFVEMFAGLGASRIRRLFREARKASPAIVFIDELDA
                     VGATRGTNDSTGEKDQTLNQLLVELDGFGGREQVVVIAASNLLDKLDPALLRPGRFDR
                     QIFVTPPDLKGRHQILDVHTRGKPLAADADLDLVARQTSGLTGADLANICNEAAIFAG
                     RAHRETSETADFQAALERVVAGMQSRRVITDHEKRVVAYHEAGHALCSELLPSVEKVH
                     RISIVPRGQALGYTLNLPAEDRYLKTKDELMDYLVVLLGGRVTEHLIFGQITTGASDD
                     LRKVHEISRSMVTDYGMGSKIQSKQLPATDYSMADATRRLIDEEQQFIADQ"
BASE COUNT          476 a         1031 c          984 g          348 t
ORIGIN      
        1 caggcgaccg cccaccaccg aggagacggc ggccatcagc gggccggggg tcacggcgaa
       61 gccggcggtc acgatcgagt agtcccagac gcgcgtgagg aacaggatgt tgcccagcag
      121 cagcgcgtag aagccgaccg agaacaccat cagcccggtc acggccacgg cgaacgagcg
      181 ggtgcggaac aggtccaggt ccagcacggg ggcggtgtgg gtgcgcgagc gcaggaccat
      241 ggccgccagg agcgcgatgc ccaccgcccc ggagccgacg atgcgggcgc tgccgtagcc
      301 ccagctctcg ccctgcacga tggccagcgc gatcaggccg acgccgccca tcagcagggc
      361 ggcgcccagc aggtcgggca ccgcgccgcg ggactcgtcg cggtactcgc gcaggatccg
      421 gcgcgccggg atcagggcgg ccaggccgat gggcacgttg atcaggaaca cccagcgcca
      481 gttggccgcg tccaccagca cgccgcccag ggacggcccg gtggccgcgg ccacggctcc
      541 ggtggcgccc cagatggacg tcgccgtggc gcgctgctcg ggcgggaact cgggcagcag
      601 gaggccgagc gaggtgggga tcaggatcgc cgcgcccacc gcctggagca cgcgcgccgc
      661 caccaggagc tccacgctcg gggccgcggc acacagcgcg gaggccagca ggaacacgcc
      721 catgcccacg aagaaggtct tgcgccggcc caccaggtcg gcggcccggc cggccgggac
      781 cagcagcgcg gcgaacacca cgttgtaggc gttcagcacc cacgacaggc cggcctcgga
      841 ggtgcccggg aacgagcgct cgatgtccgg gaaggccacg ttcacgatcg acacgtccag
      901 gaagcccatg aagacggcga ccgcggtgac cagcagcacc tgccacttgc gttgcatgac
      961 gaaacgcacc gtacaccggt cagttgcgtc atgcaactca tgtgtctagg atcacagagg
     1021 tgctcccccg cgactacgac acgcagaact gctcggtggc ccgcacgctc gaggcggtcg
     1081 gcgaccgctg gaccatgctg gtcatccgcg aggcgttcct gcgcacgcgc cggttcgagg
     1141 acttccaggc gcacctgggc atcgcgcgca acgtgctcag cgacaggctc agccggctgg
     1201 tggacgacgg catcctggac cgccgcccct accaggactc gccgccccgg ttcgagtacc
     1261 ggctgaccga gaagggcctg gacctgtggc ccgtgctgat gacgctgatg gcctggggcg
     1321 accggcacgc cgccgagcac ggaccgccgc ggctgatcgt gcaccgcgac tgcggcggga
     1381 cgctggaccg gcatctgcgc tgcgagcggt gcgggcagga cctgggcccc cgggacgtcg
     1441 aggcacagcc cggtcctggc gcggtcgcgc ttcagtaaac ggcgcgcggg gccgattacc
     1501 ctggaacgtc gtgcccttgt tcgccttcac cttccaggag ttccaggact gggcagtcat
     1561 ctggctgccg atcctgttca tggcgctgat cgcgggcacc ctgctcgtgc tgctgcggca
     1621 catgccgcgc accaagcctc aggagatcaa gcccagctcg caggactcga tcagctggga
     1681 cgacgtggcc ggcgtcgagg aggcgaagga cgagttgcgc gaggtcgtcg acttcctgcg
     1741 cgaccccgac cgcttcaagc agctcggcgc ccaggtgccc aagggcatcc tgctccacgg
     1801 cccccccggc acgggcaaga ccctgctggc caaggcggtg gcacacgagt ccaacgccca
     1861 gttcttcgcc cagtccgcgg cgtcgttcgt cgagatgttc gccggtctcg gcgcctcgcg
     1921 catccgccgg ctgttccgcg aggcccgcaa ggccagcccg gcgatcgtgt tcatcgacga
     1981 gctggacgcg gtcggcgcca cgcgcggcac caacgacagc accggtgaga aggaccagac
     2041 gctgaaccag ctgctggtgg agctggacgg cttcggcggg cgcgagcagg tcgtggtcat
     2101 cgccgcctcc aacctgctgg acaagctcga cccggcgctg ctgcgccccg gccgcttcga
     2161 ccgccagatc ttcgtcacgc cgcccgacct caagggccgc caccagatcc tcgacgtgca
     2221 cacgcgcggc aagccgctgg ccgccgacgc cgacctggac ctggtggcac gccagaccag
     2281 cggcctgacc ggcgccgacc tggccaacat ctgcaacgag gcggccatct tcgccggccg
     2341 cgcgcaccgc gagacgagcg agaccgccga cttccaggcc gcgctcgagc gcgtggtggc
     2401 cggcatgcag tcccggcgcg tgatcaccga tcacgagaag cgggtcgtgg cctaccacga
     2461 ggccggccac gcgctgtgct cggagctcct gccgtcggtc gagaaggtgc accggatctc
     2521 gatcgtgccg cgcggccagg ccctgggcta cacgctgaac ctgcccgcgg aggaccgcta
     2581 cctcaagacc aaggacgagc tcatggacta cctggtggtc ctgctgggcg gccgggtcac
     2641 cgagcacctg atcttcggcc agatcaccac cggcgcctcc gacgacctgc gcaaggtcca
     2701 cgagatcagc cggtcgatgg tcaccgacta cggcatgggc agcaagatcc agtccaagca
     2761 gctgccggcc accgactact cgatggcgga cgccacgcgc cggctgatcg acgaggagca
     2821 gcagttcatc gccgaccag
//
LOCUS       DASUPW010000400         3307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1339786, whole genome shotgun sequence.
ACCESSION   DASUPW010000400 DASUPW010000000
VERSION     DASUPW010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339786"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..1214)
                     /locus_tag="VFY44_08920"
     CDS             complement(228..1214)
                     /locus_tag="VFY44_08920"
                     /inference="COORDINATES: protein motif:HMM:NF024734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEX5910601.1"
                     /translation="MDLTSYLPAVHQHGLITWRQALAAGLDPDAIQRLAATGEWERVR
                     RGIYTHRSTWDGLDDFRGRPLARIHAARLVLEVDHHLSHDSAALVHRIGTPDPRTALV
                     HVTRDHVRGSRVDAGIKHHGAVTTASQRTQADGLPVLSLARTAVDMAREHGPAHGLAA
                     CDAALRNGVSREELSKVATAMHHWRGIRGARVAIERADPLAESYLESLGRWLVWQLGL
                     GRPECQFGLRDGDRTVWCDIRVGRHIFEPDGLLKYGIDGRPAREDLWREKTRQDFITG
                     FKLGVSRITDHDTRHPEAALRRFAREYATTVARYGTDISDLAPYVVRRRNAG"
     gene            complement(1471..1929)
                     /locus_tag="VFY44_08925"
     CDS             complement(1471..1929)
                     /locus_tag="VFY44_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5910602.1"
                     /translation="MVDVRVITAEEAPLAARLLDDFNREYDEPSPGPEWLAGHLAGLL
                     DGDDTVVLVAGEADGVGVLRLRRSLWGPALEAYLAELYVVPSLRGRGIGRALLEACMA
                     TARERGADYMDLGTSEDDVAARKLYESAGFDRTEGRPDGALSFYYERTLD"
     gene            complement(2015..>3307)
                     /locus_tag="VFY44_08930"
     CDS             complement(2015..>3307)
                     /locus_tag="VFY44_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910603.1"
                     /translation="VAVTGFVATMPYIALQLVGIQAVLEVAGLGGGDNIIAKDAPLFI
                     AFALLAAYTYSSGLRAPAVIAFVKDILIYLVIIVAVIYLPGQVGGWTEIFGAAQDKMA
                     ATNEATGKPTGSFISVDSTHWAYATLALGSALALFMYPHSVTASLSSNSRNTIRRNAA
                     ILPAYSFVLGLLALLGWVAIAAGTNPIGLDGEPNAQLVIPQLFEDMFPAWFAGVAFAA
                     IAIGALVPAAIMSIAAANTFSRNIYKEWLNPAATPAQEAKVSKLMSLVVKGFALIFVL
                     TLDKQNAINFQLLGGIWILQTFPVVVFSLYTRWFHRYALLAGWAVAMVYGTVEAYQVV
                     NPVTGKNFGGSLGMIPGLEQMGYIAMTAFVLNLVISVVLSAILNAAKVSNGEDETIPF
                     DYIADADDPRVQKDLAQHQSHEDLYGDPDDVPGNAGAR"
BASE COUNT          482 a         1120 c         1226 g          479 t
ORIGIN      
        1 gcctggggca aggcgggcga ggccggcatg gccgcgcgca tcgtcgaggc ctgcgagaac
       61 ctccgctcgg cgggcaccgc gctcggcgcc tgaccttccg caccctccgc gccgccgggg
      121 aaccagcccg gccggacgcg acaagtaacg cggtgttggt cagacgggac gaggcttcga
      181 agccccgttg cgtccgactg acaccgcgtt acttgtccgg ggcgctctca cccggcgttc
      241 cgacgccgca cgacgtacgg cgccaggtcg ctgatgtcgg tgccgtagcg cgccacggtg
      301 gtggcgtact cccgcgcgaa gcgccgcagg gcagcctccg ggtggcgggt gtcatggtcc
      361 gtgatgcgcg agacccccag cttgaaaccg gtgatgaagt cctgccgggt cttctcgcgc
      421 cagaggtcct cgcgggccgg cctcccgtcg atgccgtact tgagcagacc gtccggttcg
      481 aagatgtgac gcccgacgcg gatgtcgcac cagacggtgc ggtctccgtc cctgagcccg
      541 aactggcact cgggtctgcc cagaccgagc tgccagacca gccagcgtcc cagcgactcg
      601 aggtacgact cggcgagcgg gtcggcccgc tcgatggcca cgcgagcacc tctgatcccg
      661 cgccagtgat gcatggccgt ggcgaccttg ctcagctctt cacgcgagac accgttgcgc
      721 agcgctgcgt cgcatgcggc gagcccgtgg gccggtccgt gctcccgggc catgtccacg
      781 gccgtcctgg ccaggctcag gacgggcagg ccgtcggcct gcgtccgctg tgaggcggtg
      841 gtcacggctc cgtggtgctt gatgccggcg tcgacgcggc ttccccgcac gtggtcccgg
      901 gtgacgtgga cgagagccgt gcgtggatcg ggcgtgccga tgcggtgcac gagggccgcc
      961 gagtcgtgac tcaggtggtg gtccacctcg agcaccagac gtgcggcatg gatccgggcg
     1021 agggggcggc cgcggaagtc gtctagcccg tcccacgtcg accggtgcgt gtagatgccg
     1081 cgccgcaccc gctcccactc acccgtcgcc gcgagtcgct ggatcgcgtc gggatcgaga
     1141 ccggcggcca gcgcctggcg ccacgtgatg agcccgtgct ggtggacggc tgggaggtac
     1201 gacgtgaggt ccatggcggc tgactgcccc gcgccgcgcg gtccacgctc cacccatcgc
     1261 cggctgggga cagtcgcggt ctgagtggtg gctgtggagg gcggagggcc cgctcccggg
     1321 ggcctaccag tcaagtaacg cggtgttagt cgcgcgagac aacccgtcga cggcccgttg
     1381 cgtccgacta acaccgcgtt acttgtccgg ggcgggagag tgcggcggcg cggggcggga
     1441 gagtgcggcg cggcggcgcg cggaccggag tcagtccagc gtgcgctcgt agtagaacga
     1501 cagcgccccg tcggggcggc cctcggtccg gtcgaagccg gccgactcgt agagcttgcg
     1561 cgcggccacg tcgtcctccg acgtgccgag gtccatgtag tcggcgccgc gctcccgggc
     1621 ggtcgccatg caggcctcga ggagcgcccg gccgatgccc cggccgcgca gggacggcac
     1681 gacgtacagc tcggccaggt aggcctcgag cgccgggccc cacagcgacc ggcgcagccg
     1741 cagcacgccc accccgtcgg cctcgccggc gaccaggacg accgtgtcgt cgccgtcgag
     1801 caggccggcc aggtgcccgg ccagccactc gggaccgggt gacggctcgt cgtactcccg
     1861 gttgaagtcg tccagcagcc gcgcggcgag cggcgcctcc tcggcggtga tcacgcgcac
     1921 gtcgaccatg ctccgagcct aggtcgaaat ccgggacagc ggcagggccc gcccccgggt
     1981 gatcgggagc gggccctgtc gtgacgtacg acgctcagcg ggcgccggcg ttgcccggga
     2041 cgtcgtcggg gtcgccgtac aggtcctcgt gcgactggtg ctgcgcgagg tccttctgga
     2101 cgcgcgggtc gtccgcgtcg gcgatgtagt cgaacgggat ggtctcgtcc tcgccgttgg
     2161 acaccttggc ggcgttgagg atcgcgctga gcacgaccga gatcaccagg ttgagcacga
     2221 acgccgtcat ggcgatgtag cccatctgct ccaggcccgg gatcatcccg agcgagccac
     2281 cgaagttctt gccggtgacc gggttgacga cctggtaggc ctcgacggtg ccgtagacca
     2341 tcgcgaccgc ccagccggcg agcagggcgt agcggtggaa ccagcgggtg tagaggctga
     2401 acaccacgac ggggaaggtc tgcaggatcc agatcccgcc gagcagctgg aagttgatcg
     2461 cgttctgctt gtccagggtc agcacgaaga tcagcgcgaa gcccttgacc accagcgaca
     2521 tcagcttgga gaccttggcc tcctgcgccg gggtggcggc ggggttgagc cactccttgt
     2581 agatgttgcg gctgaacgtg ttggccgcgg cgatcgacat gatcgcggcc ggcacgagcg
     2641 cgccgatcgc gatcgcggcg aaggccacgc cggcgaacca cgccgggaac atgtcctcga
     2701 acagctgcgg gatcaccagc tgcgcgttgg gctcgccgtc gaggccgatc gggttggtgc
     2761 ccgccgcgat ggcgacccag ccgagcaggg ccagcaggcc gagcacgaag gagtaggcgg
     2821 gcaggatcgc ggcgttgcgc cggatcgtgt tgcggctgtt ggacgacagc gacgcggtca
     2881 ccgagtgcgg gtacatgaac agcgcgagcg ccgagccgag cgccagggtg gcgtaggccc
     2941 agtgggtgct gtccacggag atgaaggacc cggtgggctt gcccgtggcc tcgttggtgg
     3001 ccgccatctt gtcctgggcc gcaccgaaga tctcggtcca gccgcccacc tgcccgggca
     3061 ggtagatcac cgcgacgatg atgacgaggt agatcaggat gtccttgacg aacgcgatga
     3121 cggccggcgc gcgcaggccg ctggagtagg tgtatgccgc cagcagcgcg aacgcgatga
     3181 acagcggcgc gtccttggcg atgatgttgt cgccgccgcc gaggccggcc acctcgagca
     3241 ccgcctggat gccgacgagc tggagcgcga tgtagggcat cgtcgccacg aagccggtga
     3301 ccgccac
//
LOCUS       DASUPW010000401         4947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1756333, whole genome shotgun sequence.
ACCESSION   DASUPW010000401 DASUPW010000000
VERSION     DASUPW010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1756333"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VFY44_08935"
     CDS             complement(<1..572)
                     /locus_tag="VFY44_08935"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEX5910604.1"
                     /translation="MGRAPLKTAPWRALASIAVLAVAVALAAQAAGALGGLERATEEA
                     RFEIRGADRPDDVAVVAIDAQTFGDLREQWPFPRSMHGKVLRRLKAAGAREVVYDVQF
                     TEPSQPREDEALYRALGDTGGAVLATSESDGRGGTQVLGGDENLRAVDSRAAASDLTN
                     TKGSVTRFPREVAGLDTLATAAAKRAGGSPL"
     gene            complement(585..1523)
                     /locus_tag="VFY44_08940"
     CDS             complement(585..1523)
                     /locus_tag="VFY44_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910605.1"
                     /translation="MAGPAFGSEGKSGKEAPSVTTQSAVNVTTTDAQLRAQVKTGGDD
                     DARYRFEYGTTTGYGATTPWRELDKREASYLVTRAISGLSAGTTYNFRVVASDDDGER
                     PAVYGANQSFTTAVTPSPTPLIPDPAPGPFSGAPPQLGRTVGLAPGKGKVIVRKPGAR
                     AGVPLTAGAVVPVGSVVDATGGSLALTSALPAGRTQTGSFGGAPFTVRQGRNGYVDLY
                     LRSTSCKRARKGTAATTARRKRGKRLFGRDRGGRFRTHGRNSHATVRGTRWSVEDTCK
                     GTITRVSEGAVVVRDFAKRKSVLVKAGKRYIARPRR"
     gene            complement(1642..2013)
                     /locus_tag="VFY44_08945"
     CDS             complement(1642..2013)
                     /locus_tag="VFY44_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910606.1"
                     /translation="MRDGPIPFFAHGIIEYVGGALFVALPFLLAYDSGAATALSIVIG
                     VGLIAIAASTDGPTSLVNTIPRPAHVALDYVLAAVLIGLPFLAGFSSETAPTVVFIVV
                     GVLHLLITIGTRFRSASAPPA"
     gene            2049..2732
                     /locus_tag="VFY44_08950"
     CDS             2049..2732
                     /locus_tag="VFY44_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX5910607.1"
                     /translation="MLTVAAAQPACVALDVAANAEAHADAVRAARARLVVFPEMSLTG
                     YELDAPDLDPASADLEPLVRACAETGTLALAGAPVAGRHIATLAVDGAGARVAYRKRW
                     LGGEEVGRFEPGDPPTAIDVDGLRVGLGICKDTGVDEHVEGAAALGLDLYVAGVCHLP
                     GELAEQDRRGARIARACAAPVALAGFAGPTGWGYEATAGTSTIWSADGSVAARTGADP
                     GDFARAFGN"
     gene            2794..>4947
                     /gene="dnaE"
                     /locus_tag="VFY44_08955"
     CDS             2794..>4947
                     /gene="dnaE"
                     /locus_tag="VFY44_08955"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX5910608.1"
                     /translation="MSVAHLHVHSEYSLLDGACRIDSLAERAAAYGQPALGLTDHGVM
                     NGAVEHYKACKKHGIKPIMGLEAYYVDDRRTEAVKYERNHLTLVAQSDQGFRNLVKLT
                     SAGFLEGYKRGKANVDMELLSQYSEGVIALTGCLASRFCQRLAEDNRHEARAHADDLM
                     QVFGSDNVYFEVQKNGIALQDKVNEGIVDIAREVGRPVVGTADVHYLGREDYHNHSAL
                     LCVQTKSTLAEPKMSFDTNEFYLKDTGEMTAAFSAWPEAVPTTLEIAERCDVDIELGN
                     MLIPSYETPDGRPEGAYLRDLALDGLRERYGDPIPAEAMERLDMELEVIGRMGFDAYF
                     LIVWDFVKYAKDNGIAVGPGRGSAAGSLVSYALDITEVDPLKYGLLFERFLNPERVSM
                     PDIDIDFSVKGRARVMRYVENKYGRESVAQIVTFGKMLPRNATRDAARVLGKAYAVGD
                     RLAKLIPEPVMGRSLSLDKYLGEVDELKKAYDTDGEAREVIDTARGLEGIVRNAGVHA
                     AAVVIADRPLTDIVPLQLMEDRGAVSEDGERVYKVITQYSMGPIEELGLLKMDFLGLR
                     NLDVIENCLDIIRDAGGERPDMATLPLDDPKTYEMMARGDSVGVFQFESEGMREALRQ
                     VKPTEFEDLVALNALYRPGAMAQIPTYARNKRNPSEINYIDERLRPITEATYGVILYQ
                     EQSMQIAKAIAGFSGAEADDLRKAIGKKKREAMAAL"
BASE COUNT          720 a         1753 c         1808 g          666 t
ORIGIN      
        1 aggggagagc cgccggcgcg cttggccgcc gcggtggcca gcgtgtccag tccggccacc
       61 tcgcgcggga agcgggtcac ggagcccttg gtgttcgtca gatcggatgc ggccgcgcgc
      121 gagtcgaccg cgcgcaggtt ctcgtcgccg cccagcacct gtgtgccgcc ccgcccgtcg
      181 ctctcgctgg tggccaggac cgccccgccg gtgtcgccga gcgcccggta cagggcctcg
      241 tcctcgcgcg gctggctggg ctcggtgaac tgcacgtcgt agacgacctc gcgcgcgccg
      301 gcggccttca gccgccgcag cacctttccg tgcatggaac gcgggaacgg ccactgctcg
      361 cgcaggtcgc cgaaggtctg cgcgtcgatg gccaccacgg ccacgtcatc gggccggtcg
      421 gcgccgcgga tctcgaagcg cgcctcctcg gtggcgcgct ccagcccgcc gagcgcgccc
      481 gcagcctgtg cggcgagcgc gacggccacc gccaggacgg cgatcgaggc cagcgcgcgc
      541 cacggggcgg tcttcagcgg tgcgcgcccc atctgcccgt cgcgctagcg ccgggggcga
      601 gcgatgtagc gcttgcccgc cttgaccagc acgctcttcc tctttgcgaa gtcacggacg
      661 accacggcgc cctcgctgac cctggtgatc gtccccttgc aggtgtcctc gaccgaccag
      721 cgggtgccgc gcacggtcgc gtggctgttg cggccgtggg tccggaatcg cccgccgcgg
      781 tcgcggccga acaggcgctt gccgcgcttg cggcgtgcgg tcgtcgccgc ggtgcccttg
      841 cgggcgcgct tgcaggaggt gctgcgcagg tagaggtcga cgtagccgtt gcggccctgg
      901 cggaccgtga acggtgcgcc gccgaagctg ccggtctggg tccggcccgc gggcagggcg
      961 gaggtcagcg ccaggctgcc gccggtggcg tcgaccacgc tgccgaccgg cacgacggcg
     1021 ccggccgtga gcggcacgcc ggcacgcgcg cccggcttcc tgacgatgac cttccccttg
     1081 ccgggggcca ggccgaccgt gcggccgagc tgcggcgggg cgccgctgaa cggacccggg
     1141 gcggggtcgg ggatcagcgg ggtgggggaa ggcgtcacgg cggtcgtgaa cgactggttg
     1201 gcgccgtaca cggcggggcg ctcgccgtcg tcgtcggagg ccaccacgcg gaagttgtag
     1261 gtcgttccgg cgctgagccc ggagatcgcg cgggtgacca ggtaggaggc ctcgcgcttg
     1321 tccagctccc gccacggggt cgtggcgccg tagccggtcg tggtcccgta ctcgaagcgg
     1381 tagcgggcgt cgtcgtcgcc gccggtcttg acctgggcgc ggagctgggc gtccgtggtg
     1441 gtcacgttca ccgccgactg cgtggtgacg ctgggggctt ccttgccgct cttcccttcg
     1501 ctgccgaagg ccgggccggc cacgccgagg atgagtgagg tcccgagaag ggccgcgcag
     1561 gccggaagag tcgctcgcat ggggtcctga tcggcacccc ggggccgaac ttgaccaacc
     1621 tggacaagtg tcgggccagg cctacgccgg aggggcggag gccgaccgga agcgcgtccc
     1681 gatcgtgatc agcaggtgga gcaccccgac cacgatgaag accacggtgg gcgcggtctc
     1741 gctggagaac cccgccagga acggcagccc gatcaacacg gccgccagca cgtagtccag
     1801 ggccacgtgg gcgggccgcg ggatcgtgtt gaccaggctc gtgggaccgt cggtgctggc
     1861 cgcgatggcg atcagcccga cgccgatcac gatcgacagc gcggtggccg cgccgctgtc
     1921 gtaggccagc aggaagggga gggcgacgaa cagcgcgccc cccacgtact cgatgattcc
     1981 gtgcgcgaag aaggggatgg ggccgtcgcg catcatgcgc gcgcagctta cccgcgtacg
     2041 atcggctcgt gttgaccgtg gccgccgccc aacccgcctg cgtggcgctg gacgtggcgg
     2101 ccaacgccga ggcgcacgcg gacgcggtcc gggcggcccg ggcgcggctc gtggtgttcc
     2161 cggagatgtc gctgaccggg tacgagctcg acgcacccga tctcgacccc gcgtcggcgg
     2221 acctcgagcc cctggtccgg gcctgcgccg agaccggcac cctggcgctg gccggggcgc
     2281 ccgtggccgg ccggcacatc gccaccctgg ccgtcgacgg ggccggcgcc cgggtggcct
     2341 accgcaagcg ctggctgggc ggggaggagg tgggccggtt cgagcccggc gacccgccca
     2401 ccgcgatcga cgtggacggg ctccgggtgg gcctgggcat ctgcaaggac acgggggtcg
     2461 acgagcacgt ggagggcgcg gccgcgctcg gcctcgacct ctacgtggcc ggggtgtgtc
     2521 acctgccggg tgagctggcc gaacaggacc ggcgcggcgc ccggatcgcc cgcgcgtgcg
     2581 ccgccccggt ggcgctggcc ggcttcgccg ggcccacggg ctggggctac gaggcgaccg
     2641 ccgggacctc cacgatctgg tccgccgacg gctcggtggc ggcccgcacc ggggccgatc
     2701 ccggcgactt cgcccgtgcc ttcggcaact gaccgcaaat agcggggagg acctcggttg
     2761 ccatctggcg ccgaacgtag tctgggcgtc cgcgtgtccg tcgcccatct ccatgtccac
     2821 tccgagtact cgctgctcga cggcgcctgc cggatcgact ccctggccga gcgcgccgcc
     2881 gcctacggcc agcccgccct gggcctgacc gaccacggcg tcatgaacgg cgcggtcgag
     2941 cactacaagg cgtgcaagaa gcacgggatc aagccgatca tgggcctcga ggcctactac
     3001 gtggacgacc gcaggaccga ggcggtcaag tacgagcgca accacctgac gctggtcgcg
     3061 cagagcgacc agggcttccg caacctggtc aagctcacct cggccggctt cctggagggc
     3121 tacaagcgcg gcaaggccaa cgtggacatg gagctgctct cgcagtactc cgagggcgtg
     3181 atcgcgctca ccggctgcct ggcctcgcgc ttctgccagc gcctggccga ggacaaccgc
     3241 cacgaggccc gtgcccacgc cgacgacctg atgcaggtct tcggctccga caacgtctac
     3301 ttcgaggtcc agaagaacgg gatcgcgctc caggacaagg tcaacgaggg catcgtcgac
     3361 atcgcccgcg aggtcggccg gccggtggtg ggcacggccg acgtgcacta cctgggccgc
     3421 gaggactacc acaaccactc ggcgctgctg tgcgtgcaga ccaagagcac gctggccgag
     3481 ccgaagatgt ccttcgacac caacgagttc tacctcaagg acacgggcga gatgacggcc
     3541 gcgttctcgg cctggcccga ggcggtgccc accacgctgg agatcgccga gcgctgcgac
     3601 gtggacatcg agctgggcaa catgctcatc cccagctacg agacgccgga cgggcggccc
     3661 gagggcgcgt acctgcgcga cctggccctg gacgggctgc gcgagcgcta cggcgacccc
     3721 atccccgccg aggccatgga gcggctggac atggagctcg aggtcatcgg gcggatgggc
     3781 ttcgacgcct acttcctgat cgtctgggac ttcgtcaagt acgccaagga caacggcatc
     3841 gccgtgggcc cgggtcgtgg ctcggcggcc ggctcgctgg tgtcctacgc gctggacatc
     3901 accgaggtcg acccgctcaa gtacggcctg ctgttcgagc gcttcctgaa ccccgagcgc
     3961 gtgtccatgc cggacatcga catcgacttc tcggtcaagg gccgcgcgcg ggtcatgcgc
     4021 tacgtcgaga acaaatacgg gcgcgagtcg gtggcccaga tcgtgacctt cggcaagatg
     4081 ctgccgcgca acgccacgcg cgacgcggca cgggtgctgg gcaaggccta cgccgtcggc
     4141 gaccggctgg ccaagctcat ccccgagccg gtcatgggcc gctcgctgag cctggacaag
     4201 tacctgggcg aggtcgacga gctcaagaag gcctacgaca ccgacggcga ggcgcgcgag
     4261 gtcatcgaca ccgcccgcgg gctcgagggc atcgtgcgca acgccggcgt ccacgccgcg
     4321 gcggtggtca tcgccgaccg gccgctcacc gacatcgtgc cgctccagct gatggaggac
     4381 cgcggcgccg tgtccgagga cggcgagcgc gtctacaagg tcatcaccca gtactccatg
     4441 ggcccgatcg aggagctggg cctgctgaag atggacttcc tgggcctgcg caacctcgac
     4501 gtgatcgaga actgcctgga catcatccgc gacgccgggg gcgagcggcc cgacatggcc
     4561 acgctgcccc tggacgaccc gaagacctac gagatgatgg cccgcggcga ctcggtcggc
     4621 gtgttccagt tcgagtccga gggcatgcgc gaggcgctgc gccaggtcaa gcccacggag
     4681 ttcgaggacc tggtcgcgct caacgcgctg taccggccgg gcgccatggc gcagatcccc
     4741 acctacgcgc gcaacaagcg caaccccagc gagatcaact acatcgacga gcgcctgcgg
     4801 ccgatcaccg aggccaccta cggggtgatc ctctaccagg agcagtcgat gcagatcgcc
     4861 aaggcgatcg ccggcttctc cggggccgag gcggacgacc tgcgcaaggc catcggcaag
     4921 aagaagcgcg aggccatggc cgcgctc
//
LOCUS       DASUPW010000402         7022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_510291, whole genome shotgun sequence.
ACCESSION   DASUPW010000402 DASUPW010000000
VERSION     DASUPW010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510291"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /gene="truA"
                     /locus_tag="VFY44_08960"
     CDS             complement(<1..707)
                     /gene="truA"
                     /locus_tag="VFY44_08960"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX5910609.1"
                     /translation="MAARLLLEYHGAGFHGWATQPGRRTVQEELEKGLARIRREPVSL
                     AVAGRTDAGVHARGQVASHPGEPTPARALNGVLPADVRVLASDAAPEAFDARRDALSR
                     TYRYRVQTGSVARVHEAGRSLWWRFPLDRAVLRECAAMVAGVHDFTAFTRTQTRHTHF
                     HRDVKRSEWLDEDDGVLAYWIEADAFMRNMVRVLTGTMLEVAYGRYDLDHFEALLEGR
                     PRVEAGDTAPAHGLYLE"
     gene            complement(711..1511)
                     /locus_tag="VFY44_08965"
     CDS             complement(711..1511)
                     /locus_tag="VFY44_08965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5910610.1"
                     /translation="MNPEEAAQQTAKIWSTGDYANLSERLRPAAQALADACAVGAGQE
                     VLDVAAGDGNFAIACAAEGCAVVASDLAPGMVEKGRARTEAEGYDVEWVEANAQELPF
                     DDDRFECVGSVFGAMIAPEPEVVARELFRVVRPGNTVGMTAWAPGGRAPEMFDIGRRY
                     APGGPPEHTPDRWGDEDNVRRWFDGLANSIEFERRSVVWQGDSPEEFVAMMERSAPPQ
                     AAAKAAMPPDVYAQMRAEMVEVARGWAGGDGPFAVDADYLQFVARRRG"
     gene            1576..1896
                     /locus_tag="VFY44_08970"
     CDS             1576..1896
                     /locus_tag="VFY44_08970"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEX5910611.1"
                     /translation="MSAYAIAHLRTVDFNDEITEYLEKIDASLEPFSGRFIIHGKQPE
                     VIEEPWPGVVVAIEFPDLEAAHAWYASEAYQAILALRTENSDSSAIIVEGVPEGYRAA
                     SLVA"
     gene            1908..2285
                     /locus_tag="VFY44_08975"
     CDS             1908..2285
                     /locus_tag="VFY44_08975"
                     /inference="COORDINATES: protein motif:HMM:NF020376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HEX5910612.1"
                     /translation="MAEPRVTFLRGHESINVAGESHYQEALRAIAGDGDVRHDTEAHL
                     IPEPENEYDPNAVRVEIDGRKVGYLPRDLAPAWGPRLAELATRRRVGGCEATVVGPAQ
                     GTLGVFLRLPDPHDEPDGLARRL"
     gene            complement(2282..2635)
                     /gene="rplQ"
                     /locus_tag="VFY44_08980"
     CDS             complement(2282..2635)
                     /gene="rplQ"
                     /locus_tag="VFY44_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010285110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX5910613.1"
                     /translation="MRHGKQKGKLSRDSAHRKSLMMNLSREVIDHERIQTTEAKAKAV
                     KPELERLITLAKKGDTHSRRQAMSRLGQDKFIVYKLFEEIAPRYAERPGGYTRILKLG
                     PRKSDATEMVFLELV"
     gene            complement(2661..3653)
                     /locus_tag="VFY44_08985"
     CDS             complement(2661..3653)
                     /locus_tag="VFY44_08985"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003178382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX5910614.1"
                     /translation="MIEFQTPQISAEDVKDNRGTFTIEPLDRGFGYTFGNSLRRVLLS
                     SLGGAAVTSVRIEGVAHEFSTINGVKEDVTDIVLNLKEIVCRMHSDAAEVEAPLVVTG
                     PGEITAGDIDLPSGVEILNPETHIATLEKKTKLEIYLTVGHGRGYSPAEDNKTADQPI
                     GVIPIDSIFSPVKRVAYEVESARVGQRTDYDKLTLDIETDGSLDPHAALREAAEILIK
                     YLAIFSEPERVESLREAGAALVGAGAVNAGGDLSSLGGGGGGGAGDGMDDILIEELEL
                     GVRSYNCLKRAGVQTVGDLVQKSESELNAIPNFGRKSIEEVKETLEARGLSLRV"
     gene            complement(3714..4334)
                     /gene="rpsD"
                     /locus_tag="VFY44_08990"
     CDS             complement(3714..4334)
                     /gene="rpsD"
                     /locus_tag="VFY44_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX5910615.1"
                     /translation="MARETGPQCKLCRREGEKLFLKGERCLTDKCGIERRNYPPGDHG
                     RGRSRQSEYRVQLREKQKARRYYGVLEKQFATYYDRASRTAGVTGENLLRLLETRLDN
                     VLVRLGFAASRRQARQFIKHGHWQVNGRRVDIPSYQVRPDDVITVKPGSSADPKIREA
                     TELVSVVPAWLQADHDGLTAKVLRHPERDEIAAPVQEQLIVELYSK"
     gene            complement(4334..4732)
                     /gene="rpsK"
                     /locus_tag="VFY44_08995"
     CDS             complement(4334..4732)
                     /gene="rpsK"
                     /locus_tag="VFY44_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020449874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX5910616.1"
                     /translation="MAAQKPGRSRGRRKARKNVPHGQAHIKTSFNNTIVSLTDREGNV
                     IAWESAGGAGFKGSRKSTPFAAQVTADAAAKKGIEQGLQKVDVFVKGPGSGRDTAIRS
                     LQAAGLETGEIRDVTPQAHNGCRVRKRRRV"
     gene            complement(4732..5112)
                     /gene="rpsM"
                     /locus_tag="VFY44_09000"
     CDS             complement(4732..5112)
                     /gene="rpsM"
                     /locus_tag="VFY44_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016637793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX5910617.1"
                     /translation="MARISGINIPLNKRVEIGLTYIFGIGRSQSRALLAKSGVSPDTY
                     VKDLTDDEVLKLRAAIDEDLTVEGDLRRERSQNIKRLQEIGSYRGLRHRRGLPVNGQR
                     TKTNARTRKGPKRMQVAGKKKVTK"
     gene            complement(5124..5237)
                     /gene="rpmJ"
                     /locus_tag="VFY44_09005"
     CDS             complement(5124..5237)
                     /gene="rpmJ"
                     /locus_tag="VFY44_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX5910618.1"
                     /translation="MKVRASVKPMCEKCKVIRRNGAVLVICPNPRHKQKQG"
     gene            complement(5342..6145)
                     /gene="map"
                     /locus_tag="VFY44_09010"
     CDS             complement(5342..6145)
                     /gene="map"
                     /locus_tag="VFY44_09010"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX5910619.1"
                     /translation="MAIVIKTPAEIEAMAASGRVLARCHRLLLNKARPGVTTKELDEA
                     AEKFIRSQGGVPSFKGYHGFPGSICVSPNSMVVHGIPGDYALQRGDVLCVDIGVTLDG
                     WVSDAAVTLPIGQITPIARNLLDTTRASLFEGVEQARPGNRLGDVSHAIQARVEADGF
                     SIIRSLVGHGVGRDMHEDPQIPNFGRPGTGPELEEGMVLAIEPMVNAGGHEVRMGTDG
                     WAVYSEDGSLASHFEHTVAVTADGPRILTPWHEEEGFAAPVASRAAAQA"
     gene            complement(6148..6822)
                     /locus_tag="VFY44_09015"
     CDS             complement(6148..6822)
                     /locus_tag="VFY44_09015"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001380.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX5910620.1"
                     /translation="MSELNLILLGPPGAGKGTQAERLVEDFDLPYYATGDILRAAVSE
                     GTDLGKTAKEYMDKGDLVPDEVITGVIAERIDSDEARDGFLLDGFPRTVGQADELQRA
                     LDGMNRRLTAVLAIEADDDVVVKRLSGRRVNPKTGRTYHLEFDPPKNGEFDDVDGTKL
                     VQRDDDKEETVKHRLEVFHEQTKPLIDYYEDQGILRRFDGTRAPDEVNDHIRATLATL
                     RLEEQM"
BASE COUNT          960 a         2462 c         2450 g         1150 t
ORIGIN      
        1 tgctcgaggt acagcccgtg ggcgggcgcg gtgtcgcccg cctcgacgcg ggggcgcccc
       61 tccagcagcg cctcgaagtg gtccaggtcg tagcgcccgt aggccacctc gagcatcgtc
      121 cccgtcagga cgcgcaccat gttgcgcatg aaggcgtcgg cctcgatcca gtaggccagg
      181 accccgtcgt cctcgtccag ccactccgac cgcttcacgt cccggtggaa gtgcgtgtga
      241 cgggtctggg tgcgggtgaa cgcggtgaag tcgtggacgc cggccaccat ggccgcgcac
      301 tcgcgcagga cggcccggtc cagcgggaac cgccaccaca gcgaccggcc ggcctcgtgc
      361 acccgggcca ccgagcccgt ctgcacgcgg tagcggtagg tgcgcgagag cgcgtcgcgg
      421 cgggcgtcga aggcctcggg cgccgcgtcg gaggccagca cccgcacgtc ggcgggcagg
      481 accccgttca gcgcccgcgc cggggtcggc tcgcccggat ggctggccac ctgtccccgg
      541 gcgtgcacgc ccgcgtcggt gcggcccgcc acggccagcg acacgggctc gcgccggatc
      601 cgggcaaggc ccttctccag ctcctcctgc accgtgcgcc ggcccggctg ggtggcccag
      661 ccgtggaagc cggcgccgtg gtactccagc agtaaccggg cggccagggc tcagccgcgc
      721 cggcgggcga cgaactgcag gtagtcggcg tcgaccgcga aggggccgtc gccgcccgcc
      781 cagccgcgcg ccacctcgac catctcggcg cgcatctgcg cgtacacgtc gggcggcatg
      841 gccgccttgg ccgcggcctg cggcggcgcc gaccgctcca tcatggccac gaactcctcc
      901 ggcgagtcgc cctgccacac caccgatcgg cgctcgaact cgatcgagtt ggccaggccg
      961 tcgaaccagc gccggacgtt gtcctcgtct ccccagcggt cgggcgtgtg ctcgggcggc
     1021 cccccgggcg cgtagcggcg gccgatgtcg aacatctccg gcgccctgcc cccgggcgcc
     1081 cacgccgtca tgcccaccgt gttgccgggc cgcaccacgc ggaacagctc gcgcgccacg
     1141 acctccggct ccggggcgat catggccccg aacaccgagc ccacgcactc gaaccggtcg
     1201 tcgtcgaagg gcagctcctg ggcgttggcc tcgacccact ccacgtcgta gccctcggcc
     1261 tcggtgcggg cgcggccctt ctcgaccatc cccggtgcca ggtcggaggc caccacggca
     1321 cagccctcgg cggcgcaggc gatggcgaag ttgccgtcgc ccgcggccac gtccaggacc
     1381 tcctgcccgg cgccgacggc gcacgcgtcg gccagcgcct gggcggcggg ccgaagccgc
     1441 tccgacaggt tcgcgtagtc gccggtgctc cagatcttgg cggtctgctg tgcagcttcc
     1501 tcggggttca cgaccccgat cctaattacc tggcgggtaa tcgatcgccc gccgggccga
     1561 gcgtaggttg tgcccatgtc cgcttacgcc atagcccacc tgcgcacggt cgacttcaac
     1621 gacgagatca ccgagtacct ggagaagatc gacgcgtccc tcgagccctt ctcgggccgc
     1681 ttcatcatcc acggcaagca gccggaggtc atcgaggagc cgtggcccgg cgtcgtggtg
     1741 gccatcgagt tccccgacct cgaggccgcc cacgcctggt acgcctccga ggcctaccag
     1801 gcgatcctgg ccctgcgaac cgagaactcc gacagctcgg cgatcatcgt ggagggcgtt
     1861 cccgagggct accgggcggc gtcgctggtc gcgtagcctc ggaggggatg gcggagcccc
     1921 gtgtgacctt cctgcgcggc cacgagtcga tcaacgtggc cggtgagtcc cactaccagg
     1981 aggccctgcg cgcgatcgcc ggcgacggcg acgtccgcca cgacaccgag gcgcatctca
     2041 tccccgagcc cgagaacgag tacgacccga acgcggtccg ggtggagatc gacggccgca
     2101 aggtcggcta cctgccgcgc gacctggccc cggcctgggg gccgcgcctg gccgagctgg
     2161 ccacgcgcag gcgggtcggc ggctgcgagg ccaccgtggt gggcccggcg cagggcacgc
     2221 tgggcgtgtt cctacggctg ccggacccgc acgacgagcc ggacgggctc gcccgccgcc
     2281 tctagaccag ctcgaggaag accatctcgg tcgcgtcgga cttccgcggg ccgagcttga
     2341 ggatccgggt gtagcccccg ggccgctccg cgtagcgcgg ggcgatctcc tcgaacagct
     2401 tgtacacgat gaacttgtcc tggcccaggc gggacatcgc ctggcggcgg gagtgggtgt
     2461 cgcccttctt ggcgagcgtg atcagccgct ccagctcggg cttgaccgcc ttggccttgg
     2521 cctcggtggt ctggatgcgc tcatggtcga tgacctctcg cgagaggttc atcatcaacg
     2581 acttgcgatg ggcggaatcg cggctgagct tgcccttctg tttcccgtgg cgcatgagcg
     2641 acgttccagt ctaggcgaac tcagacccgc agcgacagcc cgcgcgcctc gagggtctcc
     2701 ttgacctctt cgatggactt gcggccgaag ttggggatcg cgttgagctc ggactcggac
     2761 ttctggacca ggtcgccgac ggtctgcacg cccgcccgct tgaggcagtt gtacgaccgc
     2821 acgcccagct ccagctcctc gatgaggatg tcgtccatgc cgtcgccggc accgccgcca
     2881 ccgccaccac cgagcgagga caggtccccg ccggcgttca cggcaccggc gcccaccagc
     2941 gcggcgccgg cctcgcgcag ggactccacg cgctcgggct cgctgaagat ggccaggtac
     3001 ttgatcagga tctcggcggc ctcgcgcagc gcggcgtggg gatccagcga cccgtcggtc
     3061 tcgatgtcca gggtcagctt gtcgtagtcg gtgcgctggc cgacacgggc cgactccacc
     3121 tcgtacgcca ctcgcttgac gggcgagaag atcgagtcga tcgggatcac gccgatgggc
     3181 tggtcggccg tcttgttgtc ctcggccggc gagtagccgc ggccgtggcc gacggtcagg
     3241 tagatctcga gcttggtctt cttctcgagc gtggcgatgt gggtctcggg gttcaggatc
     3301 tcgacgcccg agggcaggtc gatgtcaccc gcggtgatct cgccggggcc ggtgaccacc
     3361 agcggcgcct ccacctcggc ggcgtccgag tgcatccggc acacgatctc cttgaggttc
     3421 aggacgatgt cggtgacgtc ctccttgacc ccgttgatcg tggagaactc gtgggccacg
     3481 ccctcgatcc gcacgctcgt gacggcagca ccaccaagcg aagacagcag cacccgccgg
     3541 agcgagttgc cgaacgtgta gccgaagccg cggtccagcg gctcgatggt gaacgtgccg
     3601 cggttgtcct tcacgtcctc ggctgagatc tgtggagtct ggaactcgat catgtctgtc
     3661 cttcgcgttg gtgcgcgcac ggccgtggcg gcggccggcg cgacttcgac tacttacttg
     3721 gagtacagct cgacgatgag ctgctcctgc accggggcgg cgatctcgtc gcgctccggg
     3781 tgccggagca ccttcgccgt cagaccgtcg tgatcggcct gaagccaagc cgggaccacg
     3841 gagaccagct cggtggcctc acggatcttc gggtcggccg acgaccctgg cttgacggtg
     3901 atgacgtcgt ccggacgcac ctggtacgag gggatgtcga ccctgcggcc gttgacctgc
     3961 cagtgaccgt gcttgatgaa ctgacgggcc tggcggcgcg aggcggcgaa gcccaggcgc
     4021 accagcacgt tgtccagacg ggtctccagc aggcgcagca ggttctcgcc ggtcacgccg
     4081 gccgtgcgcg aggcacggtc gtagtaggtg gcgaactgct tctcgagcac gccgtagtag
     4141 cgccgcgcct tctgcttctc gcgcagctgg acgcggtact cggactggcg cgagcgtccg
     4201 cggccgtggt cgccgggcgg gtagttgcgc cgctcgatgc cgcacttgtc ggtcaggcag
     4261 cgctcgccct tcaggaagag cttctcgccc tcgcggcggc acagcttgca ctggggtccg
     4321 gtctcgcggg ccattagacg cgcctccgct tgcggacgcg gcagccgttg tgggcctgcg
     4381 gggtgacgtc gcggatctcg ccggtctcga ggccggcggc ctggagcgag cggatcgcgg
     4441 tgtcgcggcc cgagcccggc cccttgacga agacgtcgac cttctggagg ccctgctcga
     4501 tgcccttctt ggcggccgcg tcggcggtga cctgggcggc gaacggggtc gacttgcgcg
     4561 agcccttgaa accggcgccg ccggcggact cccacgcgat cacgttgccc tcacgatcgg
     4621 tcagcgacac gatcgtgttg ttgaacgaag tcttgatgtg cgcctgtccg tgggggacgt
     4681 tcttgcgcgc cttgcgccgt ccgcgggagc gccctggctt ctgagccgcc attacttggt
     4741 caccttcttc ttgcccgcca cctgcatgcg cttgggaccc ttgcgggtac gggcgttggt
     4801 cttggtgcgc tgcccgttca ccggcaggcc gcgccgatgg cgcaggccgc ggtaggaacc
     4861 gatctcctgg aggcgcttga tgttctgcga ccgctcacga cggaggtcgc cctccacggt
     4921 caggtcctcg tcgatcgcgg cgcggagctt gagcacctcg tcgtcggtca gatccttgac
     4981 gtaggtgtcg gggctcacac cgctcttggc caggagcgcc cgcgactgcg agcgcccgat
     5041 gccgaagatg taggtgagtc cgatctcgac cctcttgttg aggggaatgt tgatgcctga
     5101 gattcgagcc ataaaaggtg gtgtcaaccc tgcttctgtt tgtgccgtgg attcgggcag
     5161 atcaccagga ccgcgccgtt gcgccggatg accttgcact tctcgcacat gggcttgacc
     5221 gaggctcgga ccttcatccg tggatctacc ttccttatag agagaaccag cgaaccgccc
     5281 aggagacggc aattcgccaa cggggaaagt tagcaggcaa cgaaatcggc cgcagggcgc
     5341 tctaggcctg cgctgcggcc ctggacgcca cgggggctgc gaagccctct tcctcgtgcc
     5401 agggggtcag tatgcgcggg ccgtcggccg taacggcgac ggtgtgctcg aagtgcgagg
     5461 ccagcgatcc gtcctcggag tagacggccc atccgtcggt gcccatccgg acctcgtgcc
     5521 cgccggcgtt gaccatcggc tcgatggcca ggaccatgcc ctcctcgagc tcgggcccgg
     5581 tgcccgggcg gccgaagttg gggatctgcg ggtcctcgtg catgtcgcgc cccaccccgt
     5641 gccccaccag ggagcggatg atcgagaagc cgtcggcctc gacccgggcc tggatggcgt
     5701 gtgagacgtc gccgaggcgg ttgccgggcc gggcctgctc gacgccctcg aacagcgacg
     5761 cccgggtggt gtccagcagg ttccgcgcga tcggcgtgat ctgcccgatg ggcagggtga
     5821 ccgcggcgtc cgagacccat ccgtccagcg tcacgccgat gtccacgcac agcacgtcgc
     5881 cgcgctgcag ggcgtagtcg cccgggatgc cgtgcaccac catcgagttc ggcgacacgc
     5941 agatcgagcc cgggaagccg tgatagccct tgaacgaggg caccccgccc tgggagcgga
     6001 tgaacttctc ggcggcctcg tccagctcct tggtggtcac gccggggcgt gccttgttca
     6061 gcagcagccg gtggcagcgg gccaggacgc ggccggaggc ggccatcgcc tcgatctcgg
     6121 ccggggtctt gatgacgatg gccacggcta catctgctcc tcgaggcgaa gggtcgccag
     6181 cgtggcgcgg atgtggtcgt tcacctcgtc cggcgcgcgc gtcccgtcga accggcgcag
     6241 gatgccctgg tcctcgtagt agtcgatcag cggcttggtc tgctcgtgga agacctcgag
     6301 acggtgcttg accgtctcct ccttgtcgtc gtcgcgctgg accagcttgg tgccgtccac
     6361 atcgtcgaac tcgccgttct tgggcgggtc gaactccagg tggtaggtgc ggccggtctt
     6421 ggggttcacg cgccggccgg agagccgctt gacgaccacg tcgtcgtcgg cctcgatggc
     6481 caggaccgcg gtcagccgcc ggttcatccc gtccagcgcc cgctgcagct cgtcggcctg
     6541 gccgacggtg cgcgggaagc cgtccagcag gaagccgtcg cgggcctcgt cgctgtcgat
     6601 ccgctcggcg atgacgcccg tgatgacctc gtcgggcacc aggtcgccct tgtccatgta
     6661 ctccttggcg gtcttgccga ggtcggtgcc ctcggacacg gcggcgcgca ggatgtcgcc
     6721 ggtggcgtag tagggcaggt cgaagtcctc gaccaggcgc tcggcctggg tgcccttccc
     6781 ggcccccggc gggcccagca ggatgaggtt gagctcgctc acggagtcgg taagcgtact
     6841 ggtcgggcgc cgaggcgcca ggcacgtcct ggagctactt gaggaagccc tcgtaattgc
     6901 gcatcatcat ctgcgcctcc agctgcttca tggtgtccag cgccacgccg accacgatca
     6961 gcagcgaggt gccgccgaag gcgatgttgg tcgaggtctg ggccagcagc agggtcggga
     7021 gc
//
LOCUS       DASUPW010000403         4172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_786780, whole genome shotgun sequence.
ACCESSION   DASUPW010000403 DASUPW010000000
VERSION     DASUPW010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786780"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VFY44_09020"
     CDS             <1..309
                     /locus_tag="VFY44_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910621.1"
                     /translation="YEERPAAGDAEGLLILHHGRGADERDLLGLAGALDPQQRLHVVT
                     PGGPLTIPGWPGKHWYAVPRVGYPDPDTFRTAYDALAVFVETLELVEGLRAAGSLGPR
                     "
     gene            complement(277..930)
                     /locus_tag="VFY44_09025"
     CDS             complement(277..930)
                     /locus_tag="VFY44_09025"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910622.1"
                     /translation="MSELVYEERPAAGDAEGLLILHHGRGADEHDLLGLAGALDPQQR
                     LHVVTPGGPLTIPGWPGKHWYAVPRVGYPDPDTFWAAFQALAAFHDQTWERTGIDPGR
                     TVFGGFSMGSVMSYSLGLAGDRPVPAGIMAFSGFIPTVEGWEPSLADRAGLPVFIAHG
                     RRDPVMDVEFARRARATLTEGGLDVSYHESDVDHQIDPAHLDAATDWLAGAQAPGSA"
     gene            complement(941..2476)
                     /locus_tag="VFY44_09030"
     CDS             complement(941..2476)
                     /locus_tag="VFY44_09030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5910623.1"
                     /translation="MNRTVAMGLLFYPRGGSAQVTLYLALALRDAGWTVELLSGSLGH
                     TGDRTHAPTFFGGVASHPVDYGPAVADYEQGLDPMASPVPLHPSYEDRPGVPDRNFAA
                     VSPELAAHSAEAWTGLIRREMPPFDVAHLHHLTPMADAMRAAAPEAPVVTHLHGTELK
                     MLDRALRLTGIAALLGTSFGEMADRGAAGLPAQLDGVEGDDLALALATRWAQWRHTAF
                     WIDCMRGWAAASDRLVVVGPHDQEEAGRLLEVDASRTTWIPNGVDLERFVPLELSPDQ
                     RLQRWRRWLVEDPQAWVEGGVPGSLGYDDADLGVFTDRDSGAPAPVLMFVGRFTEVKR
                     IPLLIRAYARAREHFDRAAPLVIWGGFPGEWEGEHPHTVAMDEGVDDVFFVGWRGHDE
                     LPEGLACADVMVMPSTNESFGQVFIEAMACRVPVIAAEAGGTFAFVNVDPHDPNGWLV
                     APDDLDSLADAMVDAVNHPADRAARGASGLDLARAEYSWTTLAERTAGVYDEVVAGAA
                     TAV"
     gene            2530..3183
                     /locus_tag="VFY44_09035"
     CDS             2530..3183
                     /locus_tag="VFY44_09035"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5910624.1"
                     /translation="MSIVIIGAGPNLGQAVARRFGREGFAVGLISRTQSKLDDLASQL
                     EADGITAAGRAADIRDPDALAAAIGALADQLGPVEVLSYSPLPAPEFMKPVLETTVDD
                     VRGPLEFSVLGAIAATQAVVGPMREAGKGTILFTTGGAAVKPYPLRAGVGISFAGEVA
                     YARMLHDKLADEGVHVAHTAVGGRIGAGQDHEPDEVADVLWHHHTERGEFQSLLGID"
     gene            complement(3185..4111)
                     /locus_tag="VFY44_09040"
     CDS             complement(3185..4111)
                     /locus_tag="VFY44_09040"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX5910625.1"
                     /translation="MRPLAQPPSLLALGGGGILGEAWMTAVLAGIEDAGGFDARDSEH
                     FIGTSAGSIVAAALAGGVSAGSRVGDASRLPSLPSDDSGSESALSKLLGPALAIGGTL
                     AAPFAALALHSTTRPGAAARRAALARAPVGRRSLGDLGGMIDGNGVGWDGRLLVAAVD
                     LDSGQRQVFGADGAPEVSVSDAVQASCAIPGVFRPVEAHGRRYVDGGVWSPTNMDAVR
                     VARGDFVLCLNPTGSLPFSVGTLAGALGPVSRSIAGTEALTLRHRGARVTVVNPDRAA
                     AGAMGVNLMSPEGRGDVTAAGLAQGRRLAGAA"
BASE COUNT          555 a         1553 c         1571 g          493 t
ORIGIN      
        1 tacgaggagc gccccgcggc gggagacgcc gagggcctgc tgatcctgca ccacggccgg
       61 ggcgccgacg agcgcgacct gctgggcctg gccggcgcgc tggaccccca gcagcgcctg
      121 cacgtcgtca cccccggagg gccgttgacc atcccgggtt ggccgggcaa gcactggtac
      181 gcggtcccgc gcgtgggcta ccccgacccg gacaccttcc gcacggcgta cgacgcgctg
      241 gcggtgttcg tggagacgct cgagctggtg gaagggctac gcgctgccgg gagcctgggc
      301 ccccgctagc cagtcggtcg ccgcgtccag gtgcgcgggg tcgatctggt ggtcgacgtc
      361 ggactcgtgg tagctcacgt ccagcccgcc ctcggtcagc gtggcccgcg cacggcgggc
      421 gaactccacg tccatgaccg gatcgcggcg gccgtgcgcg atgaacaccg gcaggccggc
      481 gcggtcggcc aggctgggct cccacccctc gacggtgggg atgaagcccg agaaggccat
      541 gatcccggcg gggaccgggc ggtcaccggc caggccgagc gagtagctca tgaccgagcc
      601 catcgagaac ccgccgaaca ccgtgcgtcc cggatcgatc ccggtccgct cccaggtctg
      661 gtcgtggaag gcggccaggg cctggaacgc cgcccagaac gtgtccggat cggggtagcc
      721 cacgcgcggg accgcgtacc agtgcttgcc cggccagccc gggatggtca gcggcccgcc
      781 cggggtgacc acgtgcaggc gctgctgggg atcgagcgcg ccggccaggc cgagcaggtc
      841 gtgctcgtcg gcaccgcgcc cgtggtgcag gatcagcagc ccctcggcgt ctcccgccgc
      901 ggggcgctct tcgtagacca gctcgctcat cggctcgacg ctacacggcc gtggcggcgc
      961 ccgcgacgac ctcgtcgtag acaccggccg tgcgctcggc cagggtcgtc cacgagtact
     1021 cggcccgggc caggtcgagc ccgctggcgc cgcgggccgc ccggtcggcg gggtggttga
     1081 cggcgtccac catcgcgtcg gccaggctgt ccaggtcgtc gggcgcgacc agccagccgt
     1141 tcgggtcgtg cgggtcgacg ttgacgaagg cgaaggtgcc gcccgcctcg gccgcgatga
     1201 ccggcacgcg gcacgccatg gcctcgatga acacctggcc gaaggactcg ttggtggagg
     1261 gcatgaccat cacgtccgcg cacgccaggc cctcgggcag ctcgtcgtgg ccgcgccagc
     1321 cgacgaagaa cacgtcgtcg acgccctcgt ccatggccac cgtgtgaggg tgctcgccct
     1381 cccactcgcc cgggaagccg ccccagatga ccagcggggc cgcacggtcg aagtgctcgc
     1441 gggcgcgcgc gtaggcgcgt atgagcaggg ggatgcgctt cacctcggtg aaacgcccga
     1501 cgaacatcag caccggcgcg ggcgcgccgc tgtcgcggtc ggtgaacacg cccaggtcgg
     1561 cgtcgtcgta gcccaggctg ccggggaccc cgccctcgac ccaggcctgc gggtcctcga
     1621 cgagccaccg ccgccaccgc tgcagccgct ggtccggtga cagctcgagc ggcacgaacc
     1681 gctccaggtc gacgccgttg gggatccatg tggtgcggga cgcgtcgacc tccaggagcc
     1741 ggcccgcctc ctcctggtcg tgcgggccca cgaccaccag ccggtcgctc gcggcggccc
     1801 agccgcgcat gcagtcgatc cagaacgccg tgtggcgcca ctgcgcccag cgggtggcca
     1861 gggccaaggc caggtcgtcg ccctcgacgc cgtcgagctg ggccggcagg ccggccgcgc
     1921 cccggtcggc catctcgccg aagctggtgc ccagcagggc cgcgatgccg gtcaggcgca
     1981 gcgcccggtc cagcatcttc agctcggtcc cgtgcaggtg ggtcacgacc ggcgcctccg
     2041 gggccgccgc gcgcatggcg tcggccatgg gtgtcagatg gtgcaggtgg gccacgtcga
     2101 agggcggcat ctcacgccgg atcagcccgg tccaggcctc cgcgctgtgc gccgccagct
     2161 cgggcgacac ggcggcgaag tttcggtcgg gcaccccggg gcggtcctcg tacgacggat
     2221 gcagcgggac cggcgaggcc atcgggtcca ggccctgctc gtagtcggcg acggcggggc
     2281 cgtagtcgac gggatgggag gccacgccgc cgaagaacgt gggcgcgtgg gtgcggtcgc
     2341 cggtgtggcc cagcgagccg gacagcagct cgaccgtcca gccggcgtcg cggagcgcga
     2401 gcgccaggta cagcgtcacc tgcgccgagc cgccgcgggg gtagaagagc aaccccatgg
     2461 cgactgtccg gttcacaccg tgaacgtact cgccgttcgt aaataccgga cgcctctaac
     2521 gtcgctgtca tgtccatcgt catcatcggc gccgggccga acctgggcca ggcggtcgcc
     2581 cgccgcttcg gccgcgaggg gttcgccgtc ggcctgatct cgcgcaccca gtccaagctc
     2641 gacgacctgg cctcccagct ggaggccgac ggcatcaccg ccgccgggcg ggccgccgac
     2701 atccgcgacc ccgacgcgct ggccgccgcc atcggcgctc tggcggacca gctcggcccg
     2761 gtcgaggtcc tgtcctactc cccgctgccg gcaccggagt tcatgaagcc cgtcctggag
     2821 acgaccgtgg acgacgtgcg cggcccgctg gagttcagcg tgctgggcgc gatcgcggcc
     2881 acgcaggccg tggtcggccc gatgcgcgag gcgggcaagg gcacgatcct gttcaccacc
     2941 ggtggcgccg ccgtgaagcc gtacccgttg cgcgccggcg tggggatctc gttcgcgggc
     3001 gaggtggcct acgcgcgcat gctgcacgac aagctcgccg acgagggcgt gcacgtggcc
     3061 cacaccgccg tcggcggccg gatcggggcg ggccaggacc acgagcccga cgaggtggcc
     3121 gacgtgctct ggcaccacca caccgagcgg ggcgagttcc agtcgctgct cgggatcgac
     3181 tgagctaggc ggcgccggcc agccggcggc cctgcgccag gccggcggcg gtcacgtcgc
     3241 cccggccctc cggcgacatc aggttcacgc ccatcgcgcc cgccgcggcg cggtcggggt
     3301 tgaccaccgt cacgcgcgca ccgcggtggc gcagcgtcag ggcctcggtg cccgcgatcg
     3361 agcgcgagac gggccccagc gcgccggcca gggtgccgac cgagaagggc agcgaaccgg
     3421 tcgggttcag gcacaggacg aagtcgccgc gggcgacgcg gactgcgtcc atgttggtcg
     3481 ggctccacac gccgccgtcc acgtagcggc ggccgtgcgc ctcgaccggc cggaagacgc
     3541 cggggatggc gcacgaggcc tggacggcgt cggacaccga gacctcgggc gcgccatccg
     3601 ccccgaacac ctggcgctgg cccgagtcca ggtccacggc ggccaccagg aggcggccgt
     3661 cccagcccac gccgttgccg tcgatcatcc cgccgaggtc gcccagcgag cgccgcccga
     3721 ccggcgcgcg ggccagcgcc gcccgccggg cggcggcgcc cgggcgggtc gtggagtgca
     3781 gcgcgagcgc cgcgaacggc gcggccaggg tgccgccgat ggccagcgcg gggcccagca
     3841 gcttcgagag cgcggactcg gagccggagt cgtcgctggg cagcgacggc agcctgctgg
     3901 cgtcgcccac gcgcgagccg gcgctgacgc cgccggccag ggcggcggcg acgatcgagc
     3961 cggctgaggt gccgatgaag tgctcgctgt cgcgggcgtc gaagccgccg gcgtcctcga
     4021 tgccggccag caccgcggtc atccaggcct cgcccaggat gccgcccccg cccagcgcga
     4081 gcagggacgg tggctgggcg agcgggcgca tccgggggac gttagagcga gccggaggcc
     4141 ccggagccga ggaggtcacc ccgatacctc cg
//
LOCUS       DASUPW010000404         3630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_786943, whole genome shotgun sequence.
ACCESSION   DASUPW010000404 DASUPW010000000
VERSION     DASUPW010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786943"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="VFY44_09045"
     CDS             <1..1140
                     /locus_tag="VFY44_09045"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEX5910626.1"
                     /translation="GAAGPQGPAGADGAPGATGPAGPQGEPGVAGPQGEAGPAGAAGE
                     TGPAGPQGEAGPAGPQGEAGATGPAGPQGPRGDTGPAGAGLPTNGSFIANDDGGFAVR
                     SEQGIGTIPTTGDGNRLMWHPFKSAFRAGEAANGGMDEVNIGFYSWGGGAQSRASGAY
                     SFAFGNDAIATGLTSTALGSNVTASGNYSWALGRSANTNGQFGAFVWSDGTSGTTELM
                     ANAPNSFSIRASGGVRLFTNTGMTAGVTLTAGGSSWSVVSDRNRKESFLAVDGEDVLA
                     RIRSIPVTTWRYRDEEDRGVRHIGPMAQDWHRAFGFSGDPLTINMSDLDGVNFAAVQA
                     LEQRTAQLGGRVAEVERLSAEVAALRDENAELRARLERIEALLKP"
     gene            complement(1743..>3630)
                     /locus_tag="VFY44_09050"
     CDS             complement(1743..>3630)
                     /locus_tag="VFY44_09050"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX5910627.1"
                     /translation="GTNHHGGRVAAIFSNAGETGAGTAGVMWSSDMFITAGSDSGTVG
                     TVFKQDSVIRNDLRRAIQQRSRVINLSWGAAYFDSVTGKPRPANPAIPGDLTRANAFR
                     QFWRARMVAYEDGVAPASHPLYVIAAGNDAVDALYSGLPQLATDSRFRNRVVVVAAHD
                     STRSGSGRTLSSFSNTGSLVTVAAPGSELHFRTAAGTDWFNQPGTSFAAPHVAGLAGL
                     LFSQVPTRDAAKVRSYIIEGASRGGRTAGGFPMANAYESLKRGAEDNGAPLCGNRVWA
                     EGAQIWAQRGSGKEAIGAAESGGDVSDILTQHGGRVVLYNNTAAQGRALHRQANGTWT
                     LGTVPSDFNGKIGGATWSELAYSHGADSLVGVNTSLVNNNSWWRTGSQADVPVILRSV
                     GNSDALLGRLTIPNVPIPETRVCVERVPNGSCTYTLISGRYWLFRVAFPQAYQPVLIT
                     VTGWYSAFKEPEPWRTCTRDPSLECRNAPSELLWTPSTVYRIPIKGGTPVKVTELPDP
                     VLWIGQTEAPGSDSLVMGTGRWISEYLFNPDTRSQPRTRNEIPNCAIEYRSLATIQTV
                     AQRIENPNVCYWSSFTSPEDHGGGTFSPFRGPGTPRESTPSNAREMRIDVGELLRAEG
                     TRRR"
BASE COUNT          546 a         1236 c         1282 g          566 t
ORIGIN      
        1 ggcgctgcgg gtccacaggg tcccgcggga gcggacggcg cgccgggcgc cacgggtccc
       61 gccggaccgc agggcgagcc gggcgtcgcg gggccgcaag gagaggccgg ccccgccggt
      121 gctgccggtg agaccgggcc tgccgggccg cagggtgagg ccggaccggc cggcccccag
      181 ggtgaagcgg gcgccacggg tcccgccgga ccgcaggggc ccaggggaga caccgggccc
      241 gccggcgcgg ggctgcccac caacggctcg ttcatcgcca acgacgacgg cgggtttgcc
      301 gtccgcagcg agcagggcat cggcaccatt cccaccaccg gggacggaaa ccgcctgatg
      361 tggcatccgt tcaagtcggc gttccgcgcg ggcgaggcgg caaacggggg gatggacgag
      421 gtcaacatcg gcttctactc gtggggcggc ggcgcgcagt cgcgggcgag cggcgcgtac
      481 agcttcgcgt tcggcaacga cgccatcgcc acggggctga cgtccaccgc gctggggagc
      541 aacgtgaccg ccagcggcaa ctactcgtgg gcgctgggcc ggtccgccaa caccaacggc
      601 cagttcggcg cctttgtgtg gtccgacggg acttcgggga ccacggagct gatggcgaac
      661 gcgcccaact ccttctccat ccgcgcgtcg ggcggcgtgc ggctgttcac caacacgggg
      721 atgacggcgg gcgtgacgct cacggccggc ggcagctcgt ggagcgtggt ctcggaccgc
      781 aaccgcaagg agagctttct ggcggtggat ggagaagacg tgctggcgcg catccggtcc
      841 atccccgtca ccacctggcg ctaccgcgac gaggaggacc gcggcgtccg ccacatcggc
      901 ccgatggcgc aggactggca ccgggcgttc ggcttcagcg gcgacccgct caccatcaac
      961 atgagcgacc tggacggcgt gaacttcgcc gcggtgcagg cgctggagca gcggacggcg
     1021 cagctcggcg gccgcgtggc cgaggtggag cgcctgagcg ccgaagtcgc cgcgctgcgc
     1081 gacgagaacg ccgagctccg cgcgcggctg gagcgcatcg aggccctgct caagccctga
     1141 gtccacacgc gactttctct acgtagagcg gggccggctc cagagcacag gagccggccc
     1201 cgctctcgtt tccgaggcgt cgccgctccc gcccaaacga acagggtcaa cggggcaggg
     1261 ctttctcgtg ccgcgctaca cccttccgcc gcgtatccaa tgaacaagag cttgactctg
     1321 ttgactctgt tgactctgtg tgaggcccga tcctttgccg gagccggcct gagcggtgca
     1381 gccagcgagg tccatcgtag ttcctggaga tctgagagtg tgtttaagat cttctcattc
     1441 agaaaaccca gggttgattt cctgagggag cgcgcctcca gaacctaaag gtactcaacc
     1501 gcttgcgcgc gaccgaagga tctcatctcg gcgatcgcgg ggctggccac gcggcactga
     1561 tccagccccc aggtgctgcc ggcgaggtcc ttcggtcggc gctaaattgc ttgcgcggca
     1621 gcgggttggg agcggcgcct ccctcaggaa atcaatcggc aggttgatcg aaacacgctg
     1681 aaaggagagg ccccgcaacg gtcgtccgtt gcggggcctt ccattgcccg ggcgtgcggt
     1741 ggctatctgc gccgtgtgcc ttcagcgcgc agcaactcgc cgacgtcgat cctcatctct
     1801 cgtgcgttcg atggggtact ctcgcgtggc gtcccggggc cgcggaacgg ggagaaggtc
     1861 ccgccgccgt ggtcttccgg gctcgtgaac gacgaccagt agcacacgtt cgggttttcg
     1921 atccgctggg ccaccgtctg gatggtggcg agcgaccggt actcgatggc gcaattgggg
     1981 atctcgttcc tggtcctcgg ctggctgcgc gtgtccggat tgaacaggta ctcgctgatc
     2041 cacctgccgg tgcccatcac cagcgagtcg gaacccggcg cttcggtctg cccgatccac
     2101 aggacgggat ccgggagctc ggtgaccttt accggcgtgc cgcccttgat ggggatcctg
     2161 tagaccgtgg acggcgtcca cagcagctca gagggcgcat tgcggcactc caggctgggg
     2221 tcacgcgtgc aggttctcca gggctccggc tccttgaacg cgctgtacca gccggtcacc
     2281 gtgatcagga cgggctggta cgcctgcgga aaggcgaccc ggaacagcca gtagcgcccg
     2341 gagatcaggg tataggtgca cgagccgttg ggcacgcgct ccacgcagac ccgggtctcc
     2401 ggtatgggca cgttcgggat cgtcagcctt cccagcagtg cgtcggagtt tccgaccgag
     2461 cggaggatga ccggcacgtc ggcctggctt cccgtgcgcc accacgagtt gttgttcacg
     2521 agcgacgtat tcactcccac cagggagtcg gcgccgtgcg agtaggcgag ctccgaccac
     2581 gtggccccgc cgatctttcc gttgaagtcc gagggaacgg tacccagcgt ccacgtgccg
     2641 ttggcctgcc ggtgcagcgc ccgcccctgc gcggccgtgt tgttgtacag gaccacccgg
     2701 ccgccgtgct gggtgaggat gtcggacacg tcgccgccgc tttccgccgc gccgatcgcc
     2761 tccttgccac tcccgcgctg ggcccagatc tgcgctccct cggcccacac gcggttgccg
     2821 cacagcggcg cgccgttgtc ctcggccccg cgcttgagcg actcgtacgc gttagccatg
     2881 gggaacccgc ccgccgtccg gccgccgcgg ctcgcgccct cgatgatgta cgagcgcacc
     2941 ttggcggcgt cgcgcgtggg cacctgcgag aacagcagcc ccgccaggcc ggccacgtgt
     3001 ggcgcggcaa agctggttcc cggctggttg aaccagtcgg tgcccgcggc ggtgcggaag
     3061 tgaagctccg agcccggggc cgccacggtc accagggagc ccgtgttgga gaacgacgac
     3121 aacgtgcggc cgctccccga gcgcgtggaa tcgtgcgcgg cgacgacgac gacgcggttc
     3181 ctgaaacggg agtcggtcgc gagctgggga aggccgctgt acagggcgtc gaccgcatcg
     3241 ttccccgcgg cgatcacgta caggggatgg ctggcgggcg cgacgccgtc ctcgtacgcc
     3301 accatccgcg cgcgccagaa ctgccggaac gcgttcgccc gggtgaggtc gccggggatc
     3361 gccgggttcg cgggacgcgg ctttccggtg acggagtcga agtacgccgc gccccacgac
     3421 aggttgatca cgcgcgaccg ctgctgaatg gcccgtcgga ggtcgttgcg gatcaccgaa
     3481 tcctgcttga agacggtacc gaccgttccg ctgtcggatc cggcggtgat gaacatgtca
     3541 ctgctccaca tcacgcccgc tgttccggca cccgtctcac cggcattgct gaaaatggcc
     3601 gcgaccctgc cgccgtggtg attggttccc
//
LOCUS       DASUPW010000405         6843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_373105, whole genome shotgun sequence.
ACCESSION   DASUPW010000405 DASUPW010000000
VERSION     DASUPW010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6843
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373105"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..137)
                     /locus_tag="VFY44_09055"
     rRNA            complement(<1..137)
                     /locus_tag="VFY44_09055"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(559..1731)
                     /gene="tyrS"
                     /locus_tag="VFY44_09060"
     CDS             complement(559..1731)
                     /gene="tyrS"
                     /locus_tag="VFY44_09060"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX5910628.1"
                     /translation="MSDSPGEAARFLTRNAVDALPEGRLEKQLAEGRPLRAKLGVDPT
                     APDIHLGHTVVLRKLREFQDLGHTVVLIVGDYTARVGDPSGRSATRPQLDGAEIDANA
                     TTFKEQAFAVLDPDRTEVRSNGEWLDMPTEDLFRLARTATVAQILERDDFAKRYSARE
                     PISVLELLYPLLQGYDSVAVDADVELGGTDQKFNLLLARDIQQDYGHEPQSILTMPIL
                     PGTDGVQKMSKSLGNYVGVTEPAEEIFGKLMRVPDDAMPVYYDLLLDEPFDPGRPAVE
                     SKRAMARALAARFHGQEAAAAAEAHFDRLHVAREMPDEIEDYAFAANGGDVHVPALLR
                     GAFGISGSEARRLLTQGGVRLDGDPLGAGDMDVPAERLEGSVLQVGKRRFRRLRAG"
     gene            1866..4202
                     /locus_tag="VFY44_09065"
     CDS             1866..4202
                     /locus_tag="VFY44_09065"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX5910629.1"
                     /translation="MTEPPTTITPSHVPSPDASHASANGGGPPSPPKPRLKKLRLALM
                     LVVLGALAVVSTVFGMMMAVASDLPQLENRAEYRRAENSTLLGEPTGKGKKPEDLAKL
                     TGNQNRILVKDSDISTNMENAVIAIEDRRFYEHEGVDYKGITRALSQDILRRSAVQGG
                     STITQQFVKNALAAQSKRTVFQKLREAALAYHLERKWTKQKVLTQYMNSVYFGNGAYG
                     VEAAARTYFGGSTDTTTTPTGLPADAATGGTQGPGLRGRVARDLTPAQAAMLAGIIAS
                     PSLYDPVENPRNAKKRRDHVLGNMLDQKMITRSQYDSSIREALPTEREITPPRPESRQ
                     PYFTTWLTQQLVDRYKAPAVFSGGLKVQTTLDPELQAAAEKAINGRLNGVGPSASLVA
                     IENKTGKVRAMVGGTDYQNRPFNLATNGHRQPGSSFKPFTLIEALDNGVDPNSTFSSQ
                     KKEFPVPGSPGEKFVVNNYEDSYSGVSSLSSATATSDNSVYAELGLKVGTKKIADLAQ
                     KMGVRTKVSDNPAMTLGGLKEGVTPLELAYAYTTLANHGVRVSGSLASSPMGPVAIDS
                     VTSDGRDDHNERKTERVFPAKVGQVAEGMLAGVVQGGTGKAAQIGEFAAGKTGTTENY
                     GDAWFVGYNRQMTVAVWVGYPDGLKQMKTEYRGGPVAGGTYPTEIWHDFMTSFITVRD
                     RRDAERGKEREPEEAPAAPSTSVPAVPQQEQSAAAPTQTPEQGQGGQDAAPDQAPQRT
                     PTPRQPSTPAQPQQPAPQPPAQAPAPAPGAGGGAAPTP"
     gene            complement(4192..4773)
                     /locus_tag="VFY44_09070"
     CDS             complement(4192..4773)
                     /locus_tag="VFY44_09070"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX5910630.1"
                     /translation="MTRPLEPAFYDRPVVEVAREMVGCHLRHQGAGGRIVETEAYHFT
                     EPACHAHAGVTPRTRVLHGPPGRAYVYLSYGIHSLLNAVCEDDGIGAAVLIRAIEPLD
                     GLAEMAVRRGRSARRELCNGPGKLTQALGIGLAMNGSALDGPVEILPGEGPPPRLAVG
                     ERIGITKAVELPWRFCDADSDCVSSPRLRLATA"
     gene            complement(4770..6137)
                     /gene="argH"
                     /locus_tag="VFY44_09075"
     CDS             complement(4770..6137)
                     /gene="argH"
                     /locus_tag="VFY44_09075"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX5910631.1"
                     /translation="MSRFSEPQDPLFAALNTSVGFDFRLAVYDIQQSLAHAQMLARSG
                     IIAGEDLEAIERGMAQVRSEVDNNRFVVDPLDEDVHMAIERRLTEIVGEPGAKLHTAR
                     SRNDQVATDVAMLVRAHCLRAQDLLQALMGTLVQLAEDHLDWPMPGYTHLQRAQPVYL
                     SHHLLAYFWKFRRDLQRFTFCLTGTDDLPLGAGALAGVNFDTDRQYVARELGFTGVSP
                     NSLDAVSNRDFVLDYLSAAATCSTHLSQLGGEIVAWSSQEFGFCEVTDRFASGSSLMP
                     QKKNPDAAELLRAKAPRVLGRLTTLHGVLHGLPLTYNKDLQEDKEQLFDAIDTVELCL
                     EVADAMLAGVKFDRQAMAAAAADEFPAATDVADMLVKRGVPFRQAHGTVGAVVRFAVD
                     QGRQLSELTREELAELAPQLDDPDAFYALLGDGAWLESKASEGGTALARVQEQLAKAR
                     AVLGE"
     gene            6183..6797
                     /locus_tag="VFY44_09080"
     CDS             6183..6797
                     /locus_tag="VFY44_09080"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5910632.1"
                     /translation="MSERPTVRPFTAGDEQATVPLLYESSGGMYDRLAGNRALAERTL
                     ARALEREETTASADVVWVAELDGEIAGAMAVMPYAEWTPRAHRFLRTTLRTVPPWRWP
                     RALWLYRSSGRMAPEPSPSSLYIDSLATATGHRRRGVARAMLEEAERLARRQGLRAGA
                     LDTWQDNRAARALYVGAGFEEVAYTPATGSLPGGVSMIKELRGR"
BASE COUNT         1042 a         2464 c         2432 g          905 t
ORIGIN      
        1 catcggggca cgttacccac gtgttactca cccgttcgcg gctctgcccc agggcaagcc
       61 ctggttcgtc gctccacttg catgtgttaa gcacgccgcc agcgttcgtc ctgagccagg
      121 atcaaactct ccgtgaagat cgggcactgg ccgaagccgg cgcccggatg attcaggaaa
      181 gctgtcgatc tccgtactaa ccgggtcccg cgtacacatg gcggtgaccc tcggactcga
      241 cgggtttcta cttactcagt taagcccttc gcgacgttgc gccgaagcgc agcgaccgaa
      301 aggtcgaacc tggacacgat cgaaagccaa aaggccttcg gtcgtacatg ctgctgagtt
      361 ttcaaagacc gccgtgctct ctgggcgggg tttccgcctc gggaacgacg ttccggcatg
      421 aaaaaggcct ctctcggagg cccgccgcgc tgccccgtct tgcgacgaga actacggccg
      481 agccatctcc aagttggact ggttcattcc gggatcggtg agtatagcgc tgcggcggcg
      541 gaacggaagg ccgggttctc agccggccct cagacggcgg aaacgccgct tgcccacctg
      601 caggaccgag ccctccagcc gctcggcggg cacgtccatg tcgcccgccc ccagcgggtc
      661 gccgtccagg cgcacgccgc cctgcgtcag cagccggcgc gcctcggatc ccgagatccc
      721 gaacgccccg cgcaggagcg ccgggacgtg gacgtccccg ccgttggccg cgaaggcgta
      781 gtcctcgatc tcgtccggca tctcgcgcgc gacgtgcaga cggtcgaagt gggcctcggc
      841 cgcggccgcg gcctcctggc cgtggaaccg ggcggcgagc gcccgggcca tcgcccgctt
      901 ggactccacc gcgggacggc cgggatcgaa gggctcgtcg aggagcaggt cgtagtagac
      961 cggcatcgcg tcgtcgggga cccgcatgag cttgccgaag atctcctcgg ccggctcggt
     1021 cacgcccacg tagttgccca gcgacttgga catcttctgc acgccgtcgg tcccgggcag
     1081 gatgggcatg gtcaggatcg actggggctc gtgcccgtag tcctgctgga tgtcgcgcgc
     1141 caggagcagg ttgaacttct ggtcggtgcc gcccagctcc acgtcggcgt ccaccgcgac
     1201 cgagtcgtag ccctggagca gcgggtagag cagctccagc accgagatcg gctcgcgcgc
     1261 ggagtagcgc ttggcgaagt cgtcgcgctc gaggatctgc gcgaccgtcg cggtccgggc
     1321 caggcggaac aggtcctcgg tgggcatgtc cagccactcc ccgttggagc ggacctcggt
     1381 cctgtcgggg tcgaggaccg cgaaggcctg ttccttgaag gtcgtggcgt tggcgtcgat
     1441 ctcggccccg tccagctggg ggcgggtggc ggagcgcccg ctggggtccc ctacacgcgc
     1501 ggtgtagtca ccgacgatca agacgaccgt gtgcccgagg tcctggaact cgcgcagctt
     1561 gcgcagcacg acggtgtgcc ccaggtggat gtcgggcgcg gtcggatcga cccccagctt
     1621 ggcccgcagc ggccgccctt cggccagctg cttctcgagc cggccctccg ggagcgcgtc
     1681 gacggcattg cgggtgagaa aacgggctgc ttcacccggg ctatccgaca tggggccaat
     1741 gctagactgc cgccccgtcg cccagggggg ccggaggccg ccgcccgcgg cttctccaag
     1801 gccccacaac gcaacccgcc gcttgggccc gcgttcaggc attcgccctt cgccgggcca
     1861 ttccgatgac ggagccgccg acaacaatca cgccctcaca cgtcccgtcg ccggacgcgt
     1921 cgcacgcgtc cgcgaacggc ggcggcccgc cctcgcctcc caagccgcgg ctcaagaagc
     1981 tgcgcctggc gctgatgctg gtcgtgctgg gcgccctggc cgtggtctcg acggtcttcg
     2041 ggatgatgat ggccgtcgcc agcgacctcc cccagctgga gaaccgcgcc gagtaccgcc
     2101 gggccgagaa ctccaccctg ctgggtgagc ccaccggcaa gggcaagaag cccgaggacc
     2161 tggcgaagct gaccggcaac cagaaccgga tcctggtcaa ggactccgac atctccacca
     2221 acatggagaa cgcggtgatc gcgatcgagg accgccgctt ctacgagcac gagggcgtcg
     2281 actacaaggg catcacgcgc gccctgtccc aggacatcct gcgccgcagc gccgtgcagg
     2341 gcggctcgac gatcacccag cagttcgtga agaacgcgct ggccgcgcag tccaagcgca
     2401 cggtgttcca gaagctgcgc gaggccgcgc tggcctacca cctggagcgc aagtggacca
     2461 agcagaaggt gctgacccag tacatgaaca gcgtctactt cggcaacggc gcctacggcg
     2521 tcgaggcggc ggcgcgcacc tacttcggcg gcagcaccga caccacgacc acgccgaccg
     2581 gcctgcccgc ggacgccgcc accggcggca cccagggccc cggcctgcgc ggtcgcgtgg
     2641 cgcgggacct gacacccgcg caggcggcga tgctggcggg catcatcgcc tctcccagcc
     2701 tgtacgaccc ggtcgagaac ccgcgcaacg cgaagaagcg gcgcgaccac gtgctgggga
     2761 acatgctcga ccagaagatg atcacccgga gccagtacga cagctcgatc cgggaggcgc
     2821 tgcccaccga gcgtgagatc accccgccgc ggcccgagtc gcgccagccc tacttcacca
     2881 cctggctgac acagcagctg gtggaccgct acaaggcgcc ggccgtgttc agcggcggcc
     2941 tgaaggtcca gaccacgctc gaccccgagc tgcaggccgc ggccgagaag gccatcaacg
     3001 gccgcctgaa cggggtcggg ccgagcgcgt cgctggtggc gatcgagaac aagaccggca
     3061 aggtccgggc gatggtgggc ggcaccgact accagaaccg ccccttcaac ctggccacga
     3121 acggccaccg ccagcccggc tcgtcgttca agccgttcac gctgatcgag gcgctggaca
     3181 acggcgtgga ccccaactcc accttcagct cgcagaagaa ggagttcccg gttcccggct
     3241 cgccgggcga gaagttcgtg gtcaacaact acgaggactc ctactccggg gtgtcgtcgc
     3301 tgtcgtcggc cacggcgacg tccgacaact cggtgtacgc cgagctgggc ctgaaggtgg
     3361 gcacgaagaa gatcgccgac ctggcgcaga agatgggcgt gcggaccaag gtctcggaca
     3421 acccggcgat gacgctgggc ggcctgaagg agggcgtcac cccgctcgag ctggcctacg
     3481 cctacacgac gctggccaac cacggcgtca gggtgtcggg ctcgctggcc tcgtcgccga
     3541 tgggcccggt ggccatcgac agcgtgacca gcgacggccg cgacgaccac aacgagcgca
     3601 agaccgagcg ggtgttcccc gcgaaggtcg ggcaggtggc cgagggcatg ctggccggcg
     3661 tggtccaggg cgggaccggc aaggcggccc agatcggcga gttcgccgcc ggcaagaccg
     3721 gcaccaccga gaactacggc gacgcgtggt tcgtgggcta caaccgccag atgaccgttg
     3781 ccgtgtgggt gggctacccc gacgggctca agcagatgaa gaccgagtac cgcggcgggc
     3841 cggtggccgg cggcacgtac ccgaccgaga tctggcacga cttcatgacc tccttcatca
     3901 ccgtgcgcga ccgccgcgac gccgagcggg gcaaggagcg cgagcccgag gaggcccccg
     3961 ccgccccctc gacgtccgtc cccgccgtgc cccagcagga gcagtccgcg gcggcgccca
     4021 cgcagacccc cgagcagggc cagggcggcc aggacgcggc cccggaccag gcgccgcagc
     4081 gcacacccac accgcgccag ccctccaccc cggcccagcc ccagcagccg gcgccccagc
     4141 cgcccgcgca ggcgcccgcc ccggcccccg gggccggcgg cggcgccgcc cctacgccgt
     4201 agccaggcgc agccgcggcg acgacacgca gtcgctgtcg gcgtcgcaga agcgccaggg
     4261 cagctccacg gccttggtga tgccgatccg ctcgcccacg gccaggcgcg gcgggggtcc
     4321 ctcgcccggc aggatctcga ccggcccgtc cagggcgctg ccgttcatgg ccaggccgat
     4381 gcccagggcc tgggtgagct tgcccgggcc gttgcacagc tcgcggcgcg cgctgcggcc
     4441 ccggcggacg gccatctcgg cgagcccgtc cagcggctcg atcgcccgga tcagcaccgc
     4501 ggcgccgatg ccgtcgtcct cgcacacggc gttcaggagc gagtggatgc cgtaggagag
     4561 gtacacgtag gcccggccgg gcggcccgtg cagcacccgg gtgcggggag tcacgccggc
     4621 gtgggcgtgg cacgcgggct cggtgaagtg gtaggcctcg gtctcgacga tccggccgcc
     4681 cgcgccctgg tggcgcaggt ggcagccgac catctcgcgg gccacctcca caacggggcg
     4741 gtcgtagaag gccggctcga gcggccgcgt cactcgccca ggaccgcgcg ggccttggcc
     4801 agctgctcct ggacccgggc cagcgccgtg ccgccctctg aggccttgga ctccagccag
     4861 gccccgtccc ccagcagcgc gtagaaggcg tcggggtcgt ccagctgggg cgccagctcg
     4921 gccagctcct cgcgcgtgag ctcggacagc tgccgtccct ggtcgacggc gaaccggacc
     4981 accgcgccca cggtcccgtg ggcctggcgg aacggcacgc cgcgcttgac cagcatgtcg
     5041 gccacgtcgg tggctgccgg gaactcgtcg gcggccgccg cggccatggc ctggcggtcg
     5101 aacttgacgc cggccagcat ggcgtcggcc acctccaggc acagctcgac ggtgtcgatg
     5161 gcgtcgaaga gctgctcctt gtcctcctgg agatccttgt tgtaggtcag cggcagcccg
     5221 tgcaggaccc cgtggagggt ggtcagccgg cccagcacgc gcggcgcctt ggcgcgcagc
     5281 agctcggcgg cgtcggggtt cttcttctgc ggcatcaggc tggagccgga cgcgaaccgg
     5341 tcggtgacct cgcagaagcc gaactcctgg ctggaccagg ccacgatctc gccgcccagc
     5401 tgggacaggt gggtcgaaca ggtggcggcg gccgacaggt agtccaggac gaagtcccgg
     5461 ttggagaccg cgtcgagcga gttcggcgag acgccggtga acccgagctc acgggccacg
     5521 tactggcggt cggtgtcgaa gttcaccccc gccagcgccc ccgcgcccag cgggaggtcg
     5581 tcggtgccgg tcaggcagaa ggtgaaccgc tggaggtcgc ggcggaactt ccagaagtag
     5641 gccagcaggt ggtgcgagag gtacacgggc tgggcgcgct ggaggtgggt gtagcccggc
     5701 atgggccagt ccaggtggtc ctcggccagc tggaccagcg tgcccatgag cgcctggagc
     5761 aggtcctgcg cccgcaggca gtgcgcgcgc accagcatgg ccacgtcggt ggccacctgg
     5821 tcgttgcgcg accgcgcggt gtgcagcttg gcgcccggct cgccgacgat ctccgtcagc
     5881 cgccgctcga tggccatgtg cacgtcctcg tccagcgggt cgaccacgaa ccggttgttg
     5941 tcgacctcgg accggacctg ggccatgccg cgctcgatgg cctcgaggtc ctcgccggcg
     6001 atgatgcccg agcgggccag catctgggca tgggccagcg actgctggat gtcgtacacc
     6061 gccaggcgga agtcgaagcc caccgacgtg ttgagggcgg cgaacagcgg gtcctggggc
     6121 tcggagaagc gcgacatggc cgcgcagtct acgagcggac ggggtccgta gagtggcggg
     6181 ccgtgtcgga gcgacccacc gtgcgaccgt tcaccgcggg cgacgagcag gccacggtgc
     6241 cgctgctgta cgagagctct ggcggcatgt acgaccgcct ggccgggaac cgggcgctgg
     6301 ccgagcgcac cctggcccgc gccctggagc gcgaggagac caccgcgagc gcagacgtgg
     6361 tgtgggtggc ggagctggac ggcgagatcg cgggcgccat ggcggtcatg ccctacgcgg
     6421 agtggacgcc ccgggcccac cgcttcctgc ggaccacgct gcgcacggtc ccgccctggc
     6481 gctggccgcg cgcgctgtgg ctgtaccgct ccagcggccg gatggcgccc gagccctccc
     6541 ccagctcgct gtacatcgac tcgctggcca ccgcgaccgg gcaccggcgc cggggcgtgg
     6601 cgcgggcgat gctcgaggag gcggagcggc tggcccggcg ccagggcctg cgtgccgggg
     6661 ccctggacac ctggcaggac aaccgcgcgg cccgcgccct gtacgtgggc gccggcttcg
     6721 aggaggtcgc ctacaccccg gccaccggct cgctgcccgg cggggtgtcg atgatcaagg
     6781 agctgcgcgg ccggtagccg cggccacggc cgccgcgacg cgctggacct ggccctcggt
     6841 cat
//
LOCUS       DASUPW010000406         2965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1479993, whole genome shotgun sequence.
ACCESSION   DASUPW010000406 DASUPW010000000
VERSION     DASUPW010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479993"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /gene="rpsO"
                     /locus_tag="VFY44_09085"
     CDS             complement(<1..237)
                     /gene="rpsO"
                     /locus_tag="VFY44_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX5910633.1"
                     /translation="MSLTVEQKQEIVTKHGRGENDTGSAEVQVALLSARIEELTEHLR
                     EHRKDHHSRRGLLMLVDKRRRLLNYLQRVDLDRYR"
     gene            complement(276..1163)
                     /locus_tag="VFY44_09090"
     CDS             complement(276..1163)
                     /locus_tag="VFY44_09090"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX5910634.1"
                     /translation="MKVTSLRDIPELPPGQAPPRKVAIGTFDGVHMGHRGVIEGNDTV
                     LTFDPHPLSVLAPEKAPPLVMPHSVQRDVIGGLGVEELVVIPFDRDFSLRSAEDFVEH
                     VLVGQLGATSVSVGENFHFGRDAKGSPDFLRSRTEFETRVVPLIEVDGEPVSSTRIRE
                     LVRGGDVAGAAKLLGGPFILKGPVVHGEKRGRELGYPTANIVPREGFVAPANGVYAGW
                     ANQHLAAINVGVRPTFQSALGLLVEPYLLDFDGDLYGQELKVAFAERLRGEQKFDSVD
                     ALKEQMAADVQRTRELLAG"
     gene            complement(1160..1987)
                     /gene="truB"
                     /locus_tag="VFY44_09095"
     CDS             complement(1160..1987)
                     /gene="truB"
                     /locus_tag="VFY44_09095"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX5910635.1"
                     /translation="MIPRTADAVRAQADAVLLVDKPAGKTSHDVVAAVRREYPRKTKV
                     GHAGTLDPFATGLLLVLVGRAARLQRYLMELPKTYRAVARLGWTSDTGDPEGTLEHTG
                     RVPERLHIPEGEQLQRPPAYSAVKVDGERLYAKARRGEAVEGEPRPVTVYRAELVGRD
                     GEQATFDIECSSGTYVRQLVTGLGDAYCTELRRTAIGPFSLDDAGAELAPAEALAFMP
                     ARELDPEQARLVAHGVKLTLEEGEDVPSPVRLMHDGELVAVAEPRDRRLRPVVVFAP"
     gene            complement(1984..>2965)
                     /locus_tag="VFY44_09100"
     CDS             complement(1984..>2965)
                     /locus_tag="VFY44_09100"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEX5910636.1"
                     /translation="TEIDQVVEELRGADKLLLTTHENPDGDALGSLLGMHGVLTQLGK
                     DALMYMSPDEFPLPIEYRDMKFEGLLGAPPEDMGERVVVFLDCGNIDRMPVDFLRDEG
                     THILNIDHHHDNTRFGTANLVVPDASCTAEIVYDLARELGARITPAIADAMYVGLVTD
                     TGRFMYQNTTPAAHRMAADLIEAGVDTHDVYRRLYEDLPLRRLELLQRALASVQRYDD
                     GAITMGSLTRGDFEETGAFETDSEGIVDHMRSVEGTKVAVLSRELLAEDRQGQRKVSL
                     RATDGSVDVSVIARALGGGGHRQAAGASTSLSRDELAQEIRGLVAQQLGP"
BASE COUNT          386 a         1058 c         1076 g          445 t
ORIGIN      
        1 gcggtaccgg tccaggtcga cgcgctggag gtagttcagg agccggcggc gcttgtcgac
       61 cagcatcagc aggccacgac gcgagtggtg gtccttgcgg tgctcgcgca ggtgctcggt
      121 gagctcctcg atgcgagccg acaggagggc cacctggacc tcggccgagc cggtgtcgtt
      181 ctcaccgcgg ccgtgcttgg tgacgatctc ctgcttctgt tcaacggtca agctcatgtg
      241 cggcgcagaa aggtagcagg gcggcgggtg cggactcagc ccgccagcag ctcccgagtg
      301 cgctggacgt cggccgccat ctgctccttg agggcgtcga cggagtcgaa cttctgctcg
      361 ccgcggagcc gctcggcgaa cgccaccttc agctcctggc cgtacaggtc gccgtcgaag
      421 tcgagcaggt acggctccac caggaggccc agcgcgctct ggaaggtggg ccgcaccccc
      481 acgttgatcg cggccaggtg ttggttggcc cacccggcgt agacgccgtt ggccggggcc
      541 acgaagccct cgcgcgggac gatgttcgcg gtcgggtagc ccagctcgcg gccgcgcttc
      601 tcgccgtgca cgaccggccc cttgaggatg aacgggccgc ccagcagctt ggccgccccg
      661 gccacgtcgc cgccgcgcac cagctcgcgg atgcgggtcg aggacacggg ctcgccgtcg
      721 acctcgatca gcggcaccac gcgggtctcg aactcggtgc gcgagcgcag gaagtcgggg
      781 ctgcccttgg cgtcgcggcc gaagtggaag ttctcgccga cggagacgct ggtggcgccc
      841 agctggccca ccagcacgtg ctcgacgaag tcctcggcgc tgcgcagcga gaagtcgcgg
      901 tcgaacggga tgaccaccag ctcctccacg ccgagcccgc cgatcacgtc gcgctggacc
      961 gagtgcggca tgaccagcgg cggcgccttc tcgggggcca gcaccgacag cgggtgcggg
     1021 tcgaaggtca gcaccgtgtc gttgccctcg atgaccccgc ggtggcccat gtggacgccg
     1081 tcgaacgtgc cgatcgccac cttgcgcggg ggcgcctggc cggggggcag ctcggggatg
     1141 tcgcgcaggc tggtgacctt cacggcgcga acactacgac gggccgcagc cgccggtccc
     1201 gtggctcggc caccgcgacc agttcgccgt cgtgcatcag ccggacgggc gaggggacgt
     1261 cctccccctc ctcgagcgtc agcttgaccc cgtgcgcgac gagccgggcc tgctcggggt
     1321 ccagctcgcg cgcgggcatg aaggccagcg cctccgccgg ggccagctcg gccccggcgt
     1381 cgtccagcga gaacgggccg atcgccgtcc gccgcagctc ggtgcagtag gcgtcgccga
     1441 gccccgtgac cagctggcgc acgtaggtgc ccgacgagca ctcgatgtcg aacgtggcct
     1501 gttcgccgtc gcgcccgacc agctcggcgc ggtacacggt caccggccgc ggctcgccct
     1561 ccaccgcctc ccccctgcgc gccttcgcgt acaggcgctc gccgtcgacc ttgaccgccg
     1621 agtaggcggg cgggcgctgg agctgctcgc cctcggggat gtgcagccgc tcgggcacgc
     1681 ggcccgtgtg ctccagcgtt ccctccgggt cgccggtgtc ggaggtccag cccagccgcg
     1741 ccacggcccg gtaggtcttg ggcagctcca tcaggtagcg ctggaggcgg gccgcacggc
     1801 cgaccaacac caggagcagc ccggtggcga acgggtcgag cgtgcccgcg tggccgacct
     1861 tcgtcttgcg cgggtactcg cgccgcacgg ccgccacgac gtcgtgtgag gtcttccccg
     1921 cgggcttgtc cacgagaagg accgcgtccg cctgtgccct gacggcgtca gccgtccggg
     1981 gtatcacggc ccgagctgct gggcgacgag cccgcggatc tcctgggcca gctcgtcgcg
     2041 cgagagcgac gtggatgcgc ccgcggcctg gcggtggccg ccgccgccca gggcgcgggc
     2101 gatcacggac acgtccacgc tgccgtcggt cgcgcgcagg ctgacctttc gctggccctg
     2161 gcggtcctcg gcgagcagct cgcgcgagag caccgcgacc ttggtgccct cgaccgagcg
     2221 catgtggtcc acgatgccct cggagtcggt ctcgaatgcg ccggtctcct cgaagtcccc
     2281 ccgggtcagc gagcccatgg tgatggcgcc gtcgtcgtag cgctgcacgc tggccagcgc
     2341 gcgctggagg agctccaggc ggcgcagcgg aaggtcctcg tagaggcgcc ggtagacgtc
     2401 gtgggtgtcc accccggcct cgatcaggtc ggcggccatg cggtgggccg cgggggtggt
     2461 gttctggtac atgaaccggc ccgtgtcggt gaccaggccg acgtacatgg cgtcggcgat
     2521 ggccggggtg atccgtgccc ccagctcgcg cgccaggtcg tagacgatct cggcggtgca
     2581 ggacgcgtcc ggcaccacca ggttcgcggt gccgaagcgg gtgttgtcgt ggtggtggtc
     2641 gatgttcagg atgtgcgtgc cctcgtccct gaggaagtcg accggcatcc ggtcgatgtt
     2701 gccgcagtcc aggaacacca cgacccgctc gcccatgtcc tcggggggcg cgcccagcag
     2761 tccctcgaac ttcatgtcgc ggtactcgat cggcagcggg aactcgtccg gcgacatgta
     2821 catcagcgcg tccttgccga gctgggtcag cacgccgtgc atgccgagca gcgagccgag
     2881 cgcgtcgcca tcggggttct cgtgggtcgt cagcagcagc ttgtcggcgc cgcgcagctc
     2941 ctcgaccacc tgatcgatct cggtc
//
LOCUS       DASUPW010000407         4159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_787159, whole genome shotgun sequence.
ACCESSION   DASUPW010000407 DASUPW010000000
VERSION     DASUPW010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787159"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="VFY44_09105"
     CDS             <1..1039
                     /locus_tag="VFY44_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910637.1"
                     /translation="DPDPVYRPDPAELAEVLDEAAGDLSDEGGLVEPETIERFGLTAV
                     RARTRLMTMLHRGSTAAAPAPVEPFAPSRRGALAVRVGAGLAAGALAVAGFATLGSAP
                     TFSPLAAAAVVAVAVGVLPRLGWLAGAAAFCLWLATAGNEPGSALVLLVALAATPLLL
                     PRAGALWSLPALAPLLGLAGVAPMFVGLAALAGTAWRRAGLAAAGFVWLAVAEVLSGR
                     DLLFGVADGTLARARWEGSGTAALTDAIGPLLTSAALAPALVWAAFAVVLPLVARGRW
                     AAVDLLVGTAWAVGLVLAHDWLGQLMSTGAGLQTARGAVAGAALGALVAVTAILLAPP
                     RPDVTPEPALP"
     gene            1050..1817
                     /locus_tag="VFY44_09110"
     CDS             1050..1817
                     /locus_tag="VFY44_09110"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HEX5910638.1"
                     /translation="MSVLKNLEAKLGGLVEGAFSRAFKSRVEPVELARKLAREMEESK
                     QASVSRVYVPNHYRVFLSPQDREQFESYEPALRKELSDYLLEHARAERLALTSRPAVE
                     FMTDDRLNLGEFGIQAQQLGEPEPEPGKGLEEVAPSQGDFGHTMVYSPDRAARKLEAE
                     PVAPSRAVLSGAGRRTVLSGARVVIGRSRDCEITVDDANVSRRHAELRPQGDTWMVAD
                     LGSTNGIKVNRQRVDQAVLRHGDVVTLGLTELEFELE"
     gene            1827..2312
                     /locus_tag="VFY44_09115"
     CDS             1827..2312
                     /locus_tag="VFY44_09115"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5910639.1"
                     /translation="MDDPLAVALKFGFLAVLYLFLLWVSRSALRDLTRGQEAAGASEP
                     VDAPGGRRQSEPQDGGLLAHMSPHLTVVAALGREPGDVIDVSQGATMGRGESADINVD
                     DPFASTTHARIYARGDFMQLEDMGSTNGTYLNGRPVRGAERLKEADSIRIGDTEYRYG
                     E"
     gene            2313..3533
                     /locus_tag="VFY44_09120"
     CDS             2313..3533
                     /locus_tag="VFY44_09120"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEX5910640.1"
                     /translation="MTLRVVELAGLSDVGRQRDANEDNFVISEPLFAVADGMGGAQAG
                     EVASRTAADVFERVSGGGDRPEQLLTDLTREANRQIFEMSQSDASRRGMGTTLTAAMV
                     WSEGLSIGHVGDSRAYRLRDGKLEQLTHDHSLVAELQRSGQLTAEAAENHPQRSIITR
                     ALGPEADVEVDAHTHTTRDGDVYLLCSDGLTGMISDADIENILRGAPTLEDAAGALIK
                     AANQSGGKDNITVVLFRLGEDDGSRPLDQDTMAGSETIHQGLTADDVQAAVREQERAG
                     ARAPATGAGTPEPRGLPTHARRSRGRRALTAVVALLVVAVVAFGAYVGVRRVYFLGTD
                     DAGLVTIYRGVPYELPLGLNLFSEQYASSVPARSLGKARRERLLDHEWRSEADAEDLV
                     RQLERGTLDTGANP"
     gene            3530..>4159
                     /locus_tag="VFY44_09125"
     CDS             3530..>4159
                     /locus_tag="VFY44_09125"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX5910641.1"
                     /translation="MSARTREMLGLIPVALIVSAGFGAVILARSEGGPPGAALDEVKA
                     GTLTYGLVFLGLCLFAHLFMRARLPDADPYLFPLGATLAAFGLVVIYRIDPELAREQA
                     QWFVIGLVVFVATIVFLRDHAVLERYRYTIAAAGIALLLMPRVPGLGEQVNGAFLNVK
                     LGPISFQPAEFAKIAIIIFLASYLGDTREVLVRGRIPRLSLKHFGPLLLV"
BASE COUNT          510 a         1512 c         1574 g          563 t
ORIGIN      
        1 ggaccccgac cccgtgtacc ggcccgaccc ggccgagctg gccgaggtgc tggacgaggc
       61 cgccggcgac ctgtccgacg agggcggcct ggtcgagccc gagacgatcg agcgcttcgg
      121 cctgaccgcc gtgcgtgccc ggacacggct gatgacgatg ctgcaccggg ggagcaccgc
      181 ggcggctccg gcgccggtgg agcccttcgc tcccagccgc cgtggagcgc tggccgtccg
      241 ggtgggcgcc gggctggccg ccggcgccct ggcggtcgcg gggttcgcga cgctgggctc
      301 cgcgccgacg ttctcgccgc tggctgccgc cgccgtggtg gccgtggcgg tcggcgtgct
      361 cccgcggctc ggctggctgg ccggcgcggc cgcgttctgc ctctggctgg ccacggccgg
      421 caacgagccg gggtcggcgc tggtcctgct ggtggccctg gccgccacgc cgctcctgct
      481 gccgcgcgcc ggggcgctgt ggtcgctgcc cgcgctggcc ccgctgctgg gcctggcggg
      541 cgtggcgccg atgttcgtgg ggctggccgc gctggccggc accgcctggc ggcgcgccgg
      601 gctggccgcc gcgggcttcg tgtggctggc cgtcgccgag gtgctgagcg gccgcgacct
      661 gctgttcggc gtggccgacg gcaccctggc gcgggcgcgc tgggagggct cgggcacggc
      721 ggccctgacc gacgcgatcg ggccgctgct gacctccgcg gcgctggcgc cggccctggt
      781 gtgggccgcg ttcgccgtgg tgttgccgct ggtggcgcgg ggtcgatggg cggccgtcga
      841 cctgctcgtg ggcaccgcgt gggcggtcgg gctggtgctc gcccacgact ggctcggcca
      901 gctcatgtcc accggcgcgg ggctccagac cgcgcgcggc gccgtggcgg gcgcggccct
      961 gggtgccctg gtggccgtga cggccatcct gctggcgccg ccacggcccg acgtgacccc
     1021 ggaaccagca ctaccctgac cacccaccga tgagcgttct gaagaacctg gaagcgaagc
     1081 tcgggggcct ggtcgagggc gcgttcagtc gcgccttcaa gtcccgggtc gagccggtcg
     1141 agctggcgcg caagctcgcc cgcgagatgg aggagagcaa gcaggcctcg gtctcgcgcg
     1201 tgtacgtgcc caaccactac cgcgtcttcc tctcgccgca ggaccgcgag cagttcgaga
     1261 gctacgagcc ggcgttgcgc aaggagctct cggactacct gctcgagcac gcccgcgccg
     1321 agcggctggc gctgaccagc cggccggccg tggagttcat gaccgacgac cggctgaacc
     1381 tgggcgagtt cggcatccag gcccagcagc tgggcgagcc cgagcccgag ccgggcaagg
     1441 ggctggagga ggtggcgccg tcccaggggg acttcggcca caccatggtc tactcgcccg
     1501 atcgcgccgc ccgcaagctc gaggccgagc cggtggcgcc gtcgcgcgcc gtgctctcgg
     1561 gggccggccg gcgcacggtg ctgagcgggg cgcgcgtggt catcggccgc agccgcgact
     1621 gcgagatcac cgtggacgac gccaacgtgt cccgccgcca cgccgagctg cggccccagg
     1681 gcgacacctg gatggttgcc gacctgggct cgaccaacgg catcaaggtc aaccgtcagc
     1741 gggtcgacca ggccgtgctg cgccacggcg acgtggtcac cctgggcctg accgagctcg
     1801 agttcgagct ggagtaggcg gaccacgtgg acgatccgct ggccgtcgcg ttgaagttcg
     1861 gcttcctggc cgtgctctac ctgttcctgc tctgggtctc gcgcagcgcg ctgcgcgacc
     1921 tgacgcgggg ccaggaggcc gcgggcgcct ccgagcccgt cgacgccccc ggcgggcggc
     1981 gccagtccga gccccaggac gggggactgc tcgcgcacat gtcgccgcac ctgacggtgg
     2041 tcgcggcgct ggggcgcgag cccggtgacg tgatcgacgt gtcgcagggc gccaccatgg
     2101 gccggggcga gtcggccgac atcaacgtgg acgacccgtt cgcctccacg acccacgcgc
     2161 gcatctacgc gcgcggcgac ttcatgcagc tagaggacat gggctccacc aacgggacct
     2221 atctgaacgg ccgccccgtc cgcggcgcgg agcgcctgaa ggaggccgac tccatccgga
     2281 tcggcgacac cgagtaccga tacggcgagt agatgaccct gcgcgtcgtc gagctcgccg
     2341 gactgtccga cgttggccgc cagcgcgacg ccaacgagga caacttcgtg atctccgagc
     2401 cgctgttcgc ggtcgccgac ggcatgggcg gggcgcaggc gggcgaggtt gcgtcgcgca
     2461 ccgcagccga cgtgttcgag cgcgtcagcg ggggcggcga ccggcccgag cagctgctga
     2521 ccgacctcac ccgcgaggcc aaccgccaga tcttcgagat gtcgcagtcc gacgcctccc
     2581 ggcgcggcat gggcaccacg ctgacggcgg cgatggtgtg gagcgagggc ctcagcatcg
     2641 gccacgtggg cgacagccgc gcctaccgcc tccgggacgg caagctcgag cagctgaccc
     2701 acgaccactc gctggtggcc gagctccagc gcagcgggca gctgaccgcg gaggcggccg
     2761 agaaccaccc tcagcgctcg atcatcaccc gcgcgctggg gcccgaggcc gacgtggagg
     2821 tggacgcgca cacgcacacc acccgcgacg gcgacgtcta cctgctctgc tcggacggcc
     2881 tgacggggat gatctccgac gccgacatcg agaacatcct gcgcggcgcc ccgaccctgg
     2941 aggacgccgc cggggcgctg atcaaggccg cgaaccagag cggcggcaag gacaacatca
     3001 ccgtggtgct cttccgcctg ggcgaggacg atggctcgcg gcccctggac caggacacca
     3061 tggccgggtc ggagaccatc caccaggggc tgacggccga cgacgtgcag gccgcggtgc
     3121 gcgagcagga gcgggcgggc gccagggccc cggccaccgg cgcggggacg ccggagccgc
     3181 ggggcctgcc cacgcacgcc cgccgctcgc gcgggcgccg ggccctgacc gcggtggtcg
     3241 cgctgctggt ggtcgcggtc gtggcgttcg gggcgtacgt gggcgtgcgg cgggtctact
     3301 tcctgggcac ggacgacgcc ggcctggtga ccatctaccg cggggtgccc tacgagctgc
     3361 cgctcggcct gaacctgttc agcgagcagt acgcgtccag cgtgccggcg cggtcgctgg
     3421 gcaaggcccg gcgcgagcgg ctgctggacc acgagtggcg cagcgaggcg gacgccgagg
     3481 acctggtgcg ccagctcgag cgcggcaccc tcgacaccgg cgccaacccg tgagcgcgcg
     3541 cacgcgcgag atgctcgggc tgatcccggt ggcgctgatc gtcagcgccg ggttcggcgc
     3601 cgtgatcctg gcccgtagcg agggcgggcc gccgggcgcc gccctggacg aggtcaaggc
     3661 cggcacgctg acctacggcc tggtcttcct gggcctgtgc ctgttcgccc acctgttcat
     3721 gcgcgcgcgc ctgcccgatg ccgacccgta cctgttcccg ctgggcgcga cgctggccgc
     3781 gttcgggctc gtggtgatct accgcatcga ccccgagctg gcccgcgagc aggcccagtg
     3841 gttcgtgatc gggctggtgg tgttcgtggc cacgatcgtg ttcctgcgcg accacgcggt
     3901 gctggagcgc taccggtaca ccatcgccgc cgccgggatc gcgttgctgc tgatgccgcg
     3961 cgtgcccggg ctgggcgaac aggtcaacgg cgcgttcctg aacgtcaagc tggggccgat
     4021 ctcattccag cccgcggagt tcgccaagat cgcgatcatc atcttcctgg ccagctacct
     4081 cggcgacacg cgcgaggtac ttgtcagggg ccggatcccg cgcctctccc tgaagcattt
     4141 cgggccgctg ctcctcgtg
//
LOCUS       DASUPW010000408         4136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1340803, whole genome shotgun sequence.
ACCESSION   DASUPW010000408 DASUPW010000000
VERSION     DASUPW010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340803"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VFY44_09130"
     CDS             <1..433
                     /locus_tag="VFY44_09130"
                     /inference="COORDINATES: protein motif:HMM:NF013586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX5910642.1"
                     /translation="RMKAEFYPRVMKSRVFKEGYIAEKSGHSRGSTVDLTLVKLPQRR
                     KDGVYKPGQKLVDCAGPFAKRFKDSSVDMGTGYDCFDVRSHPLSKRITGARKANRLRL
                     RRAMVAEGFEPLSTEWWHFTLKNEPYEDTFFDFPVAKSSLR"
     gene            complement(414..1253)
                     /locus_tag="VFY44_09135"
     CDS             complement(414..1253)
                     /locus_tag="VFY44_09135"
                     /inference="COORDINATES: protein motif:HMM:NF025252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HEX5910643.1"
                     /translation="MAQPLRTPHQVPDGSPAEDFPMALSPLVDAPPARRPSAAEQART
                     IMAGTNVASLASLTREGDPWASMVTFGLVGGVPVLCLSDLALHGRNLKGDQRGSLAVA
                     VPVPQETDPSDSGRVTLAGRFEQPREDEAAAARAAYNGAVEGAEVFSAFADFTFWVMR
                     IDTVRWVGGFGRMASADPESYLAAEADPVGPHSAFAVTHLNDDHADALLLMARHLAGH
                     TDATTATCLRADRYGLDLDVSTPRGATNARVAFDQPLDAPDGLRAATVELTRRSRAIA
                     ESS"
     regulatory      1342..1509
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            1521..2159
                     /locus_tag="VFY44_09140"
     CDS             1521..2159
                     /locus_tag="VFY44_09140"
                     /inference="COORDINATES: protein motif:HMM:NF018447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HEX5910644.1"
                     /translation="MPSILVRRARSSHAAVVLALIALLCATGLVAAGAASADTAGSTG
                     AMSTEIVLKKGDRGKAVAAVQRKLGLKADGNFGAGTASAVKRFQKREGLDADGIVGPA
                     TRRAMSLPAFKSSAVTRKGAKKKRRTGSTKVPAVLAQIAECESGGDITAVSSNGRYYG
                     KYQFSKSTWKANGGQGTNPAEASEAEQDRVATKLYKAEGLTPWPSCGSKIDA"
     gene            2248..3336
                     /locus_tag="VFY44_09145"
     CDS             2248..3336
                     /locus_tag="VFY44_09145"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX5910645.1"
                     /translation="MPRALLTTLVVLLAVLAAGGPAHADQPRIRAVECIAACADGQPR
                     GGSLLLVSGTGLGNVYTAVFPGGRDGIRDLRGRAGQTSSTAVRVRVPWEAASGSFVLG
                     TRDGMVSRARRLKIAPVPVVSKWTCIGRCAPGRKVRPGSLVLVRGLRLGAVRDAVLQG
                     GRGRADDLRARVSNQRFASFRMRVPDKAITGNFSAREPRRRSPGRRLTVQAPAPVPVP
                     PGAAAGVFPVRGTHDFGSAGSRFGAGRSGHTHQGQDVMAACGVPLVSATPGTVQATGY
                     QSAAGNYLVIRGSAPVQDYVYMHLPAAPLVKKGAVVAAGQPVGAVGRSGNAQGCHLHF
                     EIWSAPGWYDGGAPFDPLPQLQAWEAAG"
     gene            3381..3509
                     /locus_tag="VFY44_09150"
     CDS             3381..3509
                     /locus_tag="VFY44_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910646.1"
                     /translation="MGETGNARGCHLHFEIWSAPGWYDGGKPFDPYPQLKAWEAAG"
     gene            complement(3506..>4136)
                     /locus_tag="VFY44_09155"
     CDS             complement(3506..>4136)
                     /locus_tag="VFY44_09155"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5910647.1"
                     /translation="AALAVPGARPGPQSSWGELRRAISGGPLRLALWLFTLPALFSGT
                     FGVLVPLRLDDLGAGGLTIGALFVAAAAVEGVLNPIMGRQSDRRGRLPLIRIGLVGMG
                     VTAVLLPLPTAVWLLGTLLLVAVGWLAFFWAPAAAMLSESSEGLGLDQGFAFGIMNLA
                     WAAGQVGGGAAGGALADLTSDAVPYGVLSLLCLVTLVLARERRPLAAAG"
BASE COUNT          595 a         1516 c         1514 g          511 t
ORIGIN      
        1 gaggatgaag gccgagttct acccgcgggt catgaagtcg cgcgtgttca aggagggcta
       61 catcgccgag aagtccgggc acagccgcgg cagcacggtg gacctgacgc tggtcaagct
      121 gccgcagagg cgcaaggacg gggtctacaa gcccggccag aagctcgtgg actgcgcggg
      181 accgttcgcc aagcggttca aggacagctc ggtggacatg ggcaccggct acgactgctt
      241 cgacgtgcgc tcacaccccc tgagcaagcg gatcacgggc gcccgcaagg ccaaccgcct
      301 gcggctgcgc cgcgcgatgg tggccgaggg cttcgagccg ctctccaccg agtggtggca
      361 cttcacgctc aagaacgagc cctacgagga cacgttcttc gacttcccgg tggctaagag
      421 ctctctgcga tagcgcggga gcgccgcgtc agctccacgg tggcggcgcg gagcccgtcc
      481 ggcgcgtcca gcggctggtc gaaggcgacg cgggcgttcg ttgcgccgcg cggggtgctc
      541 acgtccaggt ccaggccgta gcggtccgcg cgcaggcagg tggccgtcgt ggcgtcggtg
      601 tggccggcca ggtgccgggc catcaacagc agcgcatcgg cgtggtcgtc gttgagatgg
      661 gtgacggcga acgcggagtg ggggcccacg gggtccgcct cggcggccag gtagctctcg
      721 gggtcggccg acgccatgcg cccgaagccg ccgacccagc gcaccgtgtc gatccgcatg
      781 acccagaagg tgaagtcggc gaacgccgag aagacctcgg ccccttccac cgcgccgttg
      841 taggccgccc gggcggcggc ggcctcgtcc tcacgcggtt gctcgaacct tccggccagc
      901 gtgacccggc cgctgtcgga cgggtcggtc tcctgcggga ccgggaccgc cacggccagg
      961 ctgccgcgct ggtcgccctt gaggttgcgc ccgtgcaggg ccaggtcgga gaggcagagg
     1021 accggcacgc cacccacgag gccgaacgtg accatggagg cccagggatc cccctcgcgg
     1081 gtcaggctgg ccagggacgc cacgttggtg cccgccatga tcgtgcgcgc ctgctcggcc
     1141 gccgagggcc ggcgcgccgg gggggcgtcc accagcgggg acagcgccat cgggaagtcc
     1201 tccgcggggg agccatcggg gacctggtgc ggggttcgga ggggttgggc catgaagtta
     1261 gggcagccta atagaaccgg tcggaacgcg ccaggcccac ttggtggaca ttcgacctac
     1321 ggatctgctt ccataccgcc ttcgttcatt cagccgagtc gctgccagcc cgtctggcgg
     1381 cgaacggggg aaccacttgg gtccgggtcg ttcaccggtc cccggggcga atcgccgcaa
     1441 tcgcgcggcg tagcgcactt tccagcgcga gcccgtcagc tcaccccgta ggcgcttagg
     1501 aaagaggagt tgaagaaggc ttgccgtcga ttctggtccg tcgtgcccgt agttcccatg
     1561 ctgccgtcgt actcgccctg atcgcgctgc tctgcgcgac cgggctggtc gccgccggcg
     1621 ccgcctcggc cgacacggcc gggtccaccg gcgccatgag cacggagatc gtgctcaaga
     1681 agggcgaccg cggcaaggct gtggccgccg tgcagcgcaa gctcggcctc aaggccgacg
     1741 gcaacttcgg cgccggcacc gccagcgcgg tcaagcgctt ccagaagcgc gagggcctgg
     1801 acgccgacgg catcgtcggc cccgccacgc gccgcgccat gtcgctgccc gccttcaaga
     1861 gcagcgccgt gacccgcaag ggcgccaaga agaagcgcag gaccggctcg accaaggtcc
     1921 cggcggttct ggcccagatc gccgagtgcg agtccggcgg cgacatcacc gcggtctcca
     1981 gcaacgggcg gtactacggc aagtaccagt tctcgaagtc cacctggaag gccaacggcg
     2041 gccagggcac caaccccgcc gaggcctccg aggccgagca ggatcgcgtc gcgaccaagc
     2101 tgtacaaggc cgagggcctg acgccgtggc cgagctgcgg ttcgaagatc gacgcctgag
     2161 ccactccgca accttcgtcc cggacgcgtg tcggggcagg gtaagccggg acccgggcga
     2221 agccccggga tgccggtgag ctctgccatg ccccgtgcgc ttctgacaac cctggtcgtg
     2281 ctcctggccg tgctggccgc cggcgggccc gcgcatgccg accagcccag gatccgcgcg
     2341 gtggagtgca tcgccgcctg cgccgacggg cagccccgcg gtggcagcct cctgctggtg
     2401 tcgggcacgg ggctgggcaa cgtctacacg gcggtcttcc ccggcggccg cgacggcatc
     2461 cgtgacctgc gcggccgtgc gggccagacc tcctcgaccg cggtccgggt gcgggtgccg
     2521 tgggaggccg cgtccggcag cttcgtgctg ggcacccgtg acggcatggt ctcgcgcgcc
     2581 cgccggctca agatcgctcc cgtccccgtg gtctcgaagt ggacctgcat cggccggtgc
     2641 gctcccggtc gcaaggtcag gcccggcagc ctggtgctgg tgcgcggctt gcgtctcggg
     2701 gcggtgcgcg acgccgtgct gcaggggggc cgcggccgcg ccgacgacct gcgcgcgcgt
     2761 gtgagcaacc agcgcttcgc cagcttccgt atgcgcgtgc ccgacaaggc catcaccggg
     2821 aacttctcgg cacgcgagcc gcgccgccgc tcgccggggc gccggctgac cgtgcaggcc
     2881 ccggcgccgg ttcccgtgcc gcccggtgcg gcggccggcg tgttccccgt gcgcgggacc
     2941 cacgacttcg gaagcgccgg ctcgcgcttc ggcgcgggac gcagcggcca cacccaccag
     3001 ggccaggacg tcatggcggc ctgcggcgtg ccgctggtct cggccacgcc gggcacggtg
     3061 caggccaccg gttaccagtc ggccgcgggc aactacctgg tcatccgcgg gtcggccccg
     3121 gtgcaggact acgtgtacat gcacctcccg gccgccccgc tggtcaagaa gggggcggtg
     3181 gtggccgccg gccagccggt cggcgccgtg ggccggagcg gcaacgccca gggctgtcac
     3241 ctgcacttcg agatctggag cgcgccgggc tggtacgacg gcggcgcccc gttcgacccg
     3301 ctgccccagc tccaggcgtg ggaggccgcg ggctagccgg ccggcaccgg cctgcgctcg
     3361 cgggccagca gctcggcgcc gtgggcgaga ccggcaacgc gcgcggctgc cacctgcact
     3421 tcgagatctg gagcgcgccg ggctggtacg acggcggcaa gccgttcgac ccctacccgc
     3481 agctcaaggc gtgggaggcc gcgggctagc ccgcagccgc cagcggacgc cgctcgcgcg
     3541 ccagcaccag cgtgaccagg cacagcagcg agagcacgcc gtagggcacc gcgtccgagg
     3601 tcaggtcggc cagcgcgccg ccggccgcgc cgccgcccac ctgcccggca gcccaggcca
     3661 ggttcatgat cccgaaggcg aagccctggt ccaggcccag cccctcggag gactcggaca
     3721 gcatggccgc cgcgggcgcc cagaagaagg ccagccaccc cactgccacc agcagcagcg
     3781 tgcccagcag ccagaccgcg gtgggcaggg gcagcagaac cgcggtcacg cccatgccga
     3841 ccaggccgat gcggatcagc ggcagccgcc cgcggcggtc ggactggcgg cccatgatcg
     3901 ggttgagcac gccctcgacc gccgccgcgg ccacgaacag cgcgccgatg gtcagcccgc
     3961 ccgcgcccag gtcgtccagc cgcagcggca ccagcacgcc gaacgtgccc gagaacagcg
     4021 cgggcagggt gaacagccac agcgccagtc gcagcggccc gccgctgatc gccctgcgca
     4081 gctcccccca ggacgactgc gggcccgggc gcgcacccgg cacggcgagc gcagcc
//
LOCUS       DASUPW010000409         2201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_373349, whole genome shotgun sequence.
ACCESSION   DASUPW010000409 DASUPW010000000
VERSION     DASUPW010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373349"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VFY44_09160"
     CDS             <1..661
                     /locus_tag="VFY44_09160"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910648.1"
                     /translation="GPATAARLHERGIHRVEHLAAVPGAALAAMLGRAAGWHLSAIAN
                     NRDPRPVRPRTRRRSLGSQSALGTRPRSQDELDAVLIALADRVTRRMRSKGLVGRTVT
                     LRMRFGDFTRAARSRSLAQATAATETTLRTLRELYADARPLIGARGLTLLGLTFTNLG
                     HVRDGVQLALPLEEGPPQELDEVVDRVRERFGPEAVTRAALLRGGRDLAAALSPDELE
                     E"
     gene            672..1283
                     /locus_tag="VFY44_09165"
     CDS             672..1283
                     /locus_tag="VFY44_09165"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEX5910649.1"
                     /translation="MTQTIDIDTPHGPARAHLHHADRPRAALVLGHGAGGGVEAPDLK
                     AATAEAIERGVSVALVEQPYRVAGKKSSPRPNQLDPAWLAVTAHLAGHELEGLPQITG
                     GRSAGARVACRTASEGGAAGVLCLAFPLQPPARKDGSQSDSRQGELDAVEVPMLVVQG
                     VKDRFGMPRSGGDRKVVEIRGDHSLRSDAEALRHAVGEWLSIF"
     gene            1379..>2201
                     /locus_tag="VFY44_09170"
     CDS             1379..>2201
                     /locus_tag="VFY44_09170"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5910650.1"
                     /translation="MDQTTTKEAAMQTTDRNRWAALYVLCLGMLMIVLDVTVVNVALP
                     SIQDDLGFSPSNLAWVVNAYLIAFGGLLLLAGRFGDLLGRKRVFMAGMAVFTVASLAC
                     GAAQSQEMLVAARFVQGVGGAMTSAVILGMIVTMFPEPREQAKAIGIYGFVASAGGSI
                     GLLLGGVLTQSINWHWIFFVNIPIAIGTAVAAQRLLKDDQGIGFSKGADTPGAVLITS
                     SLMLGVYTIVKPGAELGFGAAQTLILGAVSVALLVGFVVREAKAENPLVPLRMFRS"
BASE COUNT          301 a          790 c          806 g          304 t
ORIGIN      
        1 cgggcccgcc accgccgccc ggttgcacga gcgcggaatc caccgcgtcg agcacctggc
       61 cgcggtcccc ggcgccgccc tggccgcgat gctgggccgc gcggcgggct ggcacctgag
      121 cgcgatcgcc aacaaccgcg acccgcggcc ggtgcgcccg cgcacccggc gccggtcgct
      181 cggcagccaa tccgcgctcg gcacccggcc gcgaagccag gacgagctgg acgcggtgct
      241 catcgcgctg gccgaccggg tcacgcgccg catgcgctcg aagggcctcg tgggccggac
      301 ggtcacgctg cggatgcgct tcggcgactt cacccgcgcc gcccggtcgc gcagcctggc
      361 ccaggccacc gcggccaccg agacgaccct gcgcacgctg cgcgagctgt acgccgacgc
      421 ccggccgctg atcggcgccc gcgggctcac gctgttgggg ctgacgttca ccaacctcgg
      481 gcacgtgcgc gacggcgtgc agttggcgct gccgctggag gagggcccgc cgcaggagct
      541 cgacgaggtg gtcgaccgcg tccgcgagcg cttcggcccg gaggccgtga cgcgagccgc
      601 gctgctgcgc ggcggccgtg acctggcggc ggcgctcagc cccgacgagc tggaggagta
      661 aggatggccg ggtgacccag accatcgaca tcgacacccc gcacgggccc gcccgcgccc
      721 acctgcacca cgccgaccgg ccccgcgccg cgctcgtgct cggccacggc gccgggggcg
      781 gggtggaggc gccggacctg aaggcggcca cggcggaggc catcgagcgt ggcgtcagcg
      841 tcgcgctcgt cgagcagccc taccgcgtgg ccggcaagaa gtcgagcccg cggcccaacc
      901 agctcgatcc cgcctggctg gccgtgaccg cccacctggc ggggcacgag ctggaggggc
      961 tgccccagat caccggcggg cgctccgccg gcgccagggt ggcgtgccgc acggcgtccg
     1021 agggcggcgc ggccggggtc ctgtgcctgg ccttcccgct ccagccccct gcgcgcaagg
     1081 acggctcgca gagcgacagc cgccagggcg agctggacgc ggtcgaggtg ccgatgctgg
     1141 tggtccaggg cgtcaaggac cggttcggga tgccccgcag cgggggcgat cggaaggtgg
     1201 tcgagatcag gggcgaccac agcctgcgca gcgacgcgga agcgctcagg catgcggtcg
     1261 gggagtggtt gtcgattttc tgagcggtgc ttcgacgtag tggtagatgg ggcgtacaga
     1321 tcgtaagaac caagcaaaga gttggaaata cctaccaact ctgatacggt cccgccccgt
     1381 ggaccagacg accaccaagg aggccgcaat gcagaccacc gaccgcaaca gatgggcagc
     1441 gctgtacgtg ctctgcctgg gcatgctcat gatcgttctc gacgtgaccg tcgtgaacgt
     1501 cgcgctgccc tcgatccagg acgacctggg cttctcgccc tcgaacctgg catgggtggt
     1561 gaacgcctac ctgatcgcgt tcggcggcct gttgctgctg gccgggcggt tcggcgacct
     1621 cctgggccgc aagcgggtct tcatggccgg catggccgtg ttcaccgtgg cctcactggc
     1681 ctgcggggcg gcacagagcc aggagatgct ggtcgcggcc cgcttcgtcc agggcgtcgg
     1741 cggcgccatg acctccgcgg tcatcctggg catgatcgtc acgatgttcc ccgagccgcg
     1801 cgagcaggcg aaggccatcg gcatctacgg gttcgtggcc tcggccggcg gctcgatcgg
     1861 cctgttgctg ggcggcgtcc tgacccagtc gatcaactgg cactggatct tcttcgtgaa
     1921 catcccgatc gccatcggca ccgcggtggc cgcgcagcgg ctgctgaagg acgaccaggg
     1981 catcggcttc tccaagggcg ccgacacccc gggtgcggtg ctgatcacca gctccctgat
     2041 gctgggcgtc tacaccatcg tcaagccggg agccgagctg ggcttcggcg ccgcgcagac
     2101 gctgatcctg ggcgccgtgt cggtcgccct cctggtgggc ttcgtggtcc gtgaggccaa
     2161 ggcggagaac ccgctggtgc cgctgcggat gttccgctcg c
//
LOCUS       DASUPW010000410         3230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1064746, whole genome shotgun sequence.
ACCESSION   DASUPW010000410 DASUPW010000000
VERSION     DASUPW010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064746"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VFY44_09175"
     CDS             <1..291
                     /locus_tag="VFY44_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEX5910651.1"
                     /translation="PKAVRASMGSVFACPPARGAATGTTVALDADAPDVLEPVDGPVT
                     LLVGAEREGLPADVVESAHVRARIPLRPGGPESLNAAMAATLALYVMSSRNG"
     gene            284..1336
                     /gene="pheS"
                     /locus_tag="VFY44_09180"
     CDS             284..1336
                     /gene="pheS"
                     /locus_tag="VFY44_09180"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016279474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX5910652.1"
                     /translation="MADAPARLEEIRAEGQAAIAAAATAAELEELRVRLLGRKAELTT
                     MLRSIKDLPPEQRGPVGKGGNQVKVVLESLLAERTAALEASELDDRLRGDAIDVTLPG
                     DPPANAGSLHLITQTRREIEDVFVGLGFSVAEGPEVEFDYYNFTALNHPPDHPARMLA
                     DTFYFSDDVLLRTHTSPMQVRSMELQEPPVYLVVPGKAYRRDNDATHTPMFHQVEGLA
                     IDEDITLADLQGVLLEFARAMFGPERDIRLRPHYFPFTEPSVEVDVSCFKCGGTGWLG
                     AERCSLCKGSGWIEILGSGMVDPNVLGFVDYDPDKVQGFAFGMGIERIAMLRHGVPDL
                     RLFYDNDVRFLAQFGA"
     gene            1338..>3230
                     /gene="pheT"
                     /locus_tag="VFY44_09185"
     CDS             1338..>3230
                     /gene="pheT"
                     /locus_tag="VFY44_09185"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX5910653.1"
                     /translation="MRVPLSWLREYCDPDLDAAGIADRLDLTGTELERIEHVGVGDTS
                     GFVVGKVLSAEQHPDADRLKVCEVDDGRGQRTIVCGAPNVAAGQTVAVALPGAVMPDG
                     TQLGEAKLRGVKSSGMICAEDELGIGEDHAGIMVLDDSWPAGAPLAEYLPLADDVLEL
                     EITPNRPDCLSVFGIAREVHAATGAELSDDPTEGDAIASGDDTSTDHVSIRIADPEIC
                     LRFTGRVFEDVKVGPSPPWLRQRLTAAGQRPISNVVDITNYVMLTCGQPLHAFDLDRV
                     RGAEIVVRRAEAGETMTTLDDVEREFSAEMAVVCDAEGPSGVAGIMGGQISEVSDATT
                     RVLMEAATWVPTNILRTSKALNLRSEASTRFEKGVHPENALAGQRLAARLMVELCGAR
                     MVPDPIDVYPEPLEPRVVRLRMERVLKLLGKAICEDEVVGRLESLGFAVRRGDGVLDV
                     TVPYWRDADVQREADLLEEVARTHGLDQLPVTLPARRNAVGRLTPRQRLRRRLEDALR
                     DRGLHEIVGWSFTAPQTLAALRLGDEPVLDLANPLSEDQSVMRPLLLPGLLDAARDNA
                     ARGAGALALFESQHVYRPSGELTAPEGSPKGALPAEESHHLAALVPGDFYAAKGLIEA
                     IGAAARV"
BASE COUNT          447 a         1156 c         1195 g          432 t
ORIGIN      
        1 ccgaaggcgg tgcgtgcctc gatgggctcg gtgttcgcgt gcccgccggc gcggggcgcc
       61 gccaccggca ccaccgtggc ccttgacgcc gatgcgcccg acgtcctgga gccggtcgac
      121 ggccccgtca cgctgctcgt gggcgccgaa cgggaaggcc tgcccgccga tgtcgttgaa
      181 tcagcccacg tgcgcgcgcg aatccccctg cggcccggcg gccccgaatc gctgaacgcg
      241 gccatggccg ccaccctggc cctctacgtg atgtcatccc gcaatggctg acgcccccgc
      301 acgcctggag gagatccgcg cggagggcca ggcggccatc gccgcagcgg ccacggccgc
      361 cgagctcgag gagctgcgcg tgcgcctgct gggccgcaag gccgagctga ccacgatgct
      421 gcgctcgatc aaggacctgc cgcccgagca gcgcggtccc gtgggcaagg gcggcaacca
      481 ggtcaaggtg gtgctggagt cgttgctggc cgagcgcacc gcggcgctgg aggcctcgga
      541 gctggacgac cggctgcgag gcgacgccat cgacgtgacg ctgcccggcg atcccccggc
      601 caacgccggg agcctgcacc tgatcacgca gacccggcgc gagatcgagg acgtgttcgt
      661 gggcctgggc ttctccgtgg ccgagggccc cgaggtcgag ttcgactact acaacttcac
      721 cgcgctgaac cacccgcccg accacccggc gcggatgctg gccgacacct tctacttctc
      781 cgacgacgtg ctgctgcgca cgcacacctc gccgatgcag gtgcgctcga tggagctcca
      841 agagccgccg gtctacctgg tggtgcccgg caaggcctac cggcgcgaca acgacgccac
      901 ccacacgccc atgttccacc aggtcgaggg gctggccatc gacgaggaca tcacgctggc
      961 ggacctccag ggcgtgctgc tggagttcgc gcgggccatg ttcggccccg agcgcgacat
     1021 ccgcctgcgc ccgcactact tcccgttcac cgagccgtcg gtcgaggtgg acgtgtcctg
     1081 cttcaagtgc ggcggcacgg gctggctggg cgccgagcgc tgctcgctgt gcaaggggtc
     1141 gggctggatc gagatcctgg gctcgggcat ggtcgacccc aacgtcctgg gcttcgtgga
     1201 ctacgacccg gacaaggtgc aggggttcgc gttcggcatg gggatcgagc ggatcgcgat
     1261 gctgcgccac ggcgtgcccg acctgcggct gttctacgac aacgacgtgc gcttcctggc
     1321 gcagttcggg gcctgagatg cgcgtcccgc tgtcatggct gcgcgagtac tgcgatcccg
     1381 acctggacgc cgccggcatc gccgaccggc tggacctgac cggcaccgag ctggagcgga
     1441 tcgagcacgt gggggtgggg gacacctccg gcttcgtggt gggcaaggtg ctcagcgccg
     1501 agcagcaccc cgacgccgac cggctgaagg tgtgcgaggt cgacgacggc cgcgggcagc
     1561 ggacgatcgt gtgcggcgcc cccaacgtgg ccgccgggca gaccgtggcc gtcgcgctgc
     1621 cgggcgcggt catgcccgac gggacccagc tgggcgaggc caagctgagg ggcgtgaagt
     1681 ccagcggcat gatctgcgcc gaggacgagc tgggcatcgg cgaggaccac gccgggatca
     1741 tggtgctgga cgactcgtgg cccgccggcg cgccgctggc cgagtatctg ccgctggccg
     1801 acgacgtgct cgagctggag atcacgccca accggcccga ctgcctctcg gtgttcggga
     1861 tcgcgcgcga ggtgcacgcc gccaccggcg ccgagctgtc cgacgacccg accgagggcg
     1921 acgccatcgc ttcgggcgac gacacctcga ccgaccacgt gtcgatccgg atcgccgacc
     1981 ccgagatctg cctgcgcttc accgggcggg tgttcgagga cgtgaaggtg gggccgtcgc
     2041 cgccgtggct gcgccagcgc ctgaccgcgg ccggccagcg gccgatctcg aacgtcgtgg
     2101 acatcaccaa ctacgtgatg ctcacctgcg gccagccgct ccacgccttc gacctggacc
     2161 gggtgcgcgg tgccgagatc gtcgtgcggc gcgcggaggc gggggagacc atgaccacgc
     2221 tggacgacgt cgagcgcgag ttcagcgccg agatggcagt ggtgtgcgac gccgagggcc
     2281 cctcgggcgt ggccgggatc atgggcgggc agatctccga ggtctcggac gccaccacgc
     2341 gtgtcctgat ggaggccgcg acgtgggtgc ccaccaacat cctgcgtacg tcgaaggcgc
     2401 tgaacctgcg ctcggaggcc tcgacgcggt tcgagaaggg cgtccacccc gagaacgccc
     2461 tggccggcca gcggctggca gcgcggctga tggttgagct ctgcggcgcc cggatggtgc
     2521 cggacccgat cgacgtgtac cccgagcctc tggagccgcg cgtggtgcgg ctgcgcatgg
     2581 agcgcgtgct caagctgctg ggcaaggcca tctgcgagga cgaggtggtg ggacggctgg
     2641 aatcgctggg cttcgccgtc cggcgcggcg acggcgtcct ggacgtgacc gtgccctact
     2701 ggcgcgacgc cgacgtgcag cgcgaggccg acctgctcga ggaggtggcc cgcacccacg
     2761 gcctggacca gctgccggtc accctgcccg cgcgccgcaa cgcggtcggg cgcctgacgc
     2821 cgcgccagcg gctgcggcgc cggctggagg acgcgctgcg tgaccgcggc ctgcacgaga
     2881 tcgtgggctg gagcttcacg gcgccccaga cgctcgcggc gctgcggctg ggcgacgagc
     2941 cggtgctgga cctggccaac ccgctcagcg aggaccagag cgtgatgcgc ccgctgttgc
     3001 tgcccgggct gctggacgcg gcgcgcgaca acgccgcccg cggcgccggc gcgctggcgc
     3061 tgttcgagtc ccagcacgtc taccgcccca gcggcgagct gaccgcgccc gagggcagcc
     3121 cgaagggcgc gctgcccgcg gaggagagcc accacctggc cgcgctcgtc cccggggact
     3181 tctacgccgc caaggggctc atcgaggcca tcggcgccgc cgcccgggtg
//
LOCUS       DASUPW010000411         3458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_648967, whole genome shotgun sequence.
ACCESSION   DASUPW010000411 DASUPW010000000
VERSION     DASUPW010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648967"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1558)
                     /locus_tag="VFY44_09190"
     CDS             complement(116..1558)
                     /locus_tag="VFY44_09190"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX5910654.1"
                     /translation="MKPPVLEDVRPRADEARALDPLTRLETLCDPGTLRLVRTAVEGP
                     SKRSRPGDGVVVGSGRVGGRTVYCYAQDSSVVGGSLGASHADSIVRVMEMAARAEAPI
                     VGFVESGGARMDEGVAALAGYGKIFRAQVELSGRVPQISVVSGTSAGGGAYSPALADF
                     VVMAPEASMFLTGPGVVNEVMGEDVTGAELGGPEVHARNGVSHLSARSDVHAVLLARE
                     LLGYLSGSGEPAPPGGDDPAATVPSSQRKVYDVREVVAALVDDGRTLETNERFAKNMV
                     TALGRIEGRSVGFVCNQPKWLGGVIDADAAQKAARFVRTCDAFGIPLVVLMDTPGFLP
                     GTRQEQAGVIRHGAKLLHAFAEATVPRMTVVLRKGYGGGFITMNSLDLGADLMLAWPD
                     AQIGVVGAAQAVKIIHRRDIAAADDPEAAAAELAGQYEADKLGAEVAAREGFVDELVE
                     PADTRDRLAWALAGSGAEGRGGRDHGNIPL"
     gene            complement(1555..2760)
                     /locus_tag="VFY44_09195"
     CDS             complement(1555..2760)
                     /locus_tag="VFY44_09195"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX5910655.1"
                     /translation="MKRRVAITGLGAVSPLGVGAGELIGRWAEGASGIADGEGRCADF
                     DPADHLSRKELRRADRFTQMALTASGEALAQAGFNGERPVSAERTACVIGTGIGGIGT
                     IEEQHLVLEHKGAAQVSPLAIPLLMANAASGSVAMKNDLRGQCYGVVSACAAGAHAIG
                     AGVRMVQYGDADACLVGGSEAAITPLASAAFGSMAAISPTGESRPFDARRDGFVMGEG
                     AGALMLEDEEAARERGAEILGVIVGYGATADAHHLTAPEPSGDGAARAIAAALEDAGI
                     APGGIDYVNAHGTSTPLNDRAETEALQTALGAHAGKIPVSSLKSSIGHLLGAAGAVEA
                     VATALALQRGVAPPTVGWEQRDEGMDLDYVPDSARPLEGDGSRPLRAISNSFGFGGHN
                     AVLCIEASS"
     gene            complement(2757..3008)
                     /locus_tag="VFY44_09200"
     CDS             complement(2757..3008)
                     /locus_tag="VFY44_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX5910656.1"
                     /translation="MSTETVSREQIESRVEDALVEFGAEREEIGPDAKFESLDVDSLD
                     LVELAQIVEEDYGVQITGEDMKTLDTVGKAVDFVEARLS"
     gene            complement(3023..>3458)
                     /locus_tag="VFY44_09205"
     CDS             complement(3023..>3458)
                     /locus_tag="VFY44_09205"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5910657.1"
                     /translation="MRMKDDDWDAVIRVNLESYFRLARASMRGMMKRRHGRIIGITSV
                     VGVTGNPGQANYAASKAGLIAMTKTVAAEVARRKVTVNAVAPGLVDTDMTEGLGDELL
                     KAVPARRVGTPEEVAACVRFLASDEAAYVTGSVLTVDGGMTA"
BASE COUNT          433 a         1348 c         1203 g          474 t
ORIGIN      
        1 gaggttgtgc aacacggtct ccgaccccag gcgttgcgcg accgcctcgg gccacggcac
       61 ctcgcccggc tcgccgacgc cggcgctgaa cgagtcgccc agcgcgacgt aggacctaga
      121 gcgggatgtt gccgtggtca cgaccacccc tcccctcggc gcctgacccg gccagcgccc
      181 acgccagccg gtcgcgcgtg tcggcgggct cgaccagctc gtccacgaac ccctcgcgtg
      241 cggccacctc ggcgcccagc ttgtcggcct cgtactggcc ggcgagctcg gccgccgcgg
      301 cctcggggtc gtcggcggcg gcgatgtcgc gccggtggat gatcttcacg gcctgcgccg
      361 cccccacgac gccgatctgg gcgtcgggcc aggccagcat caggtcggcc cccaggtcca
      421 gcgagttcat ggtgatgaag ccgccgccgt agcccttgcg cagcaccacc gtcatccgcg
      481 gcacggtggc ctcggcgaag gcgtgcagca gcttggcgcc gtggcggata acgcccgcct
      541 gctcctggcg cgtgccgggc aggaacccgg gcgtgtccat cagcaccacc agcgggatcc
      601 cgaacgcgtc gcaggtgcgc acgaaccggg cggccttctg cgccgcgtcg gcgtcgatca
      661 cgccccccag ccacttgggc tggttgcaca cgaagcccac ggagcggccc tcgatccgcc
      721 ccagcgcggt gaccatgttc ttggcgaagc gctcgttggt ctccagcgtg cggccgtcgt
      781 ccaccagcgc ggccaccacc tcgcgcacgt cgtagacctt gcgctgcgat gagggcacgg
      841 tggcggcggg gtcgtcgccg ccgggcggcg ccggctcgcc ggagccggac aggtagccca
      901 gcagctcgcg cgccagcagc accgcgtgca cgtcgctgcg cgcggacagg tgcgagacgc
      961 cgttgcgggc gtgcacctcg ggcccgccca gctccgcccc ggtcacgtcc tcgcccatga
     1021 cctcgttcac cacgccgggg ccggtcagga acatgctggc ctcgggggcc atgaccacga
     1081 agtcggccag cgcaggcgag taggcgcccc cgcccgcgga ggtcccggac accacgctga
     1141 tctggggcac gcggcccgac agctcgacct gggcgcggaa gatcttgccg tagccggcca
     1201 gcgcggccac gccctcgtcc atgcgcgcgc cgccggactc cacgaagccc acgatgggcg
     1261 cctcggcgcg ggcggccatc tccatcacgc gcacgatcga gtcggcgtgc gaggcgccga
     1321 gcgacccgcc caccacgctc gagtcctgcg cgtagcagta cacggtgcgg ccgccgacgc
     1381 ggcccgagcc cacgaccacg ccgtcgccgg gccggctgcg cttggacggc ccctcgaccg
     1441 cggtgcgcac cagccgcagc gtgccggggt cgcacagcgt ctccagccgg gtcagcgggt
     1501 ccagcgcgcg ggcctcgtcg gcccgcggcc ggacgtcctc cagcaccggc ggcttcacga
     1561 cgaggcctcg atgcacagca ccgcgttgtg cccgccgaag ccgaacgagt tggagatcgc
     1621 acgcagcgga cgggagccgt cgccctccag cggccgcgcc gagtccggca cgtagtccag
     1681 gtccatgccc tcgtcgcgct gctcccagcc cacggtcggc ggggccacgc cgcgctggag
     1741 cgccagcgcg gtggccacgg cctccacggc gccggcggcc cccagcaggt ggccgatcga
     1801 cgacttcagc gacgacaccg ggatcttgcc ggcgtgggcg cccagcgcgg tctgcagcgc
     1861 ctcggtctcg gcgcggtcgt tcaggggcgt cgacgtgccg tgggcgttca cgtagtcgat
     1921 gccgccgggc gcgatgccgg cgtcctccag cgcggccgcg atggcccgcg cggcgccgtc
     1981 gccgctgggc tcgggcgcgg tcaggtggtg ggcgtcggcg gtggcgccgt agcccacgat
     2041 gacccccagg atctcggcgc cgcgctcgcg cgcggcctcc tcgtcctcca gcatcagcgc
     2101 gcccgcgccc tcgcccatga cgaagccgtc gcggcgcgcg tcgaacggac gcgactcgcc
     2161 cgtcggcgag atggcggcca tgctcccgaa ggccgcgctg gccagcgggg tgatggcggc
     2221 ctccgagccg cccaccaggc aggcgtcggc gtcgccgtac tggaccatgc gcacgccggc
     2281 gccgatggcg tgggcgcccg ccgcgcaggc cgagaccacg ccgtagcact ggccgcgcag
     2341 gtcgttcttc atggccaccg agcccgaggc cgcgttggcc atgagcaacg ggatggccag
     2401 cggggagacc tgggccgcgc ccttgtgctc cagcaccagg tgctgctcct cgatggtgcc
     2461 gatgccgccg atgccggtgc cgatcacgca cgcggtgcgc tcggcggaca ccggcctttc
     2521 accgttgaac ccggcctggg ccagcgcctc gcccgacgcg gtcagcgcca tctgggtgaa
     2581 gcggtcggcc cgccgcagct ccttgcggct caggtggtcg gcggggtcga agtccgcgca
     2641 gcggccctcg ccgtcggcga tgcccgacgc gccctcggcc cagcggccga tcagctcgcc
     2701 ggcgcccacg cccagcgggc tgacggcgcc caggccggtt atggcgacgc gacgcttcac
     2761 gacagtcgcg cctccacgaa gtccacggcc ttgccgacgg tgtccagcgt cttcatgtcc
     2821 tcgccggtga tctggacgcc gtagtcctcc tcgacgatct gggccagctc gaccaggtcc
     2881 agcgagtcca cgtccaggga ctcgaacttg gcgtcgggcc ctatctcctc gcgctccgcg
     2941 ccgaactcga ccagcgcatc ctccacgcgg ctctcgatct gctctctgct cactgtctcc
     3001 gtgctcatct ggatgtcctc cttcaggcgg tcatgccgcc gtcgactgtc agtaccgatc
     3061 cggtcacgta tgcggcctcg tccgaggcca ggaaccgcac gcaggcggcc acctcctcgg
     3121 gggtgcccac gcggcgcgcg ggcacggcct tcagcagctc gtcgcccagc ccctcggtca
     3181 tgtcggtgtc caccaggccc ggggccacgg cgttcacggt caccttgcgg cgtgccacct
     3241 cggcggccac ggtcttggtc atcgcgatca ggccggcctt ggaggcggcg tagttggcct
     3301 ggccggggtt tccggtgacg ccgacaaccg aagtgatgcc gatgatccgg ccgtgacggc
     3361 gcttcatcat cccgcgcatg ctggcgcgcg ccagccggaa gtagctttcc aggttcaccc
     3421 tgatgacggc gtcccagtcg tcgtccttca tccgcatg
//
LOCUS       DASUPW010000412         3362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1065307, whole genome shotgun sequence.
ACCESSION   DASUPW010000412 DASUPW010000000
VERSION     DASUPW010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065307"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1444
                     /locus_tag="VFY44_09210"
     CDS             176..1444
                     /locus_tag="VFY44_09210"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEX5910658.1"
                     /translation="MAEVKPLHALHYDPESVDSFEAVIAPPYDVIDAGLRAQLLGQSP
                     KNVVEIDLPESPDGGDIYEHAAQTLEQWRADGTVVQDDEPAYWVLEQDYTAPDGSQRR
                     RRGFFARVRVEDYGPGRIRPHERTHPGPKEDRLRLTRATKANLSPIFSLFPDPGDRAG
                     KVMEDVVAGEPFARAADHEGTQNAMWRVSDPDAVAELEAALAEAELLIADGHHRYETA
                     RVYADEIGGEGDHRYVLMYLVSLSDPGLLVFPTHRLLTGLKDDALKQEAIRDTLTQDF
                     DLEEIPAEQLEPEPGDERVRMGYMDSFHKQAYALALKDQSIADKALSDKPAPYRRLDT
                     AVLEALVLKGALGMTEDDISHLKGLGYSKDFADARDAVESGRADAGFFMRATPVEQVR
                     EVAEAGESMPPKSTYFYPKVPTGLLFNPLA"
     gene            1462..2043
                     /locus_tag="VFY44_09215"
     CDS             1462..2043
                     /locus_tag="VFY44_09215"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX5910659.1"
                     /translation="MKIYTKKGDDGTTGLWYGGRVDKDSPRPEAYGSVDEAASALGMA
                     RAAAERDSEVYLDILRIQEELFIAGSELATAPEAADRLEAGVSKLTQEMVERLEQLID
                     RYMDRVDLPPKFVIPGGTELSARLDVARTFVRRAERRVESLNRAGELNDDTVLTYLNR
                     CSDAVYAIARFTDEDSPELFQGRDASTRKDSHS"
     gene            2040..2336
                     /locus_tag="VFY44_09220"
     CDS             2040..2336
                     /locus_tag="VFY44_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX5910660.1"
                     /translation="MRAIARRSGGTLKHHVEIGGHRLVADEPSEQGGTDAGPSPEELL
                     AASLASCSAITMEVQLPAGLPEEQREKLMQIVAKCPVHRTLEGEVMFEEQVSLV"
     gene            2338..2916
                     /locus_tag="VFY44_09225"
     CDS             2338..2916
                     /locus_tag="VFY44_09225"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX5910661.1"
                     /translation="MPSPDDLRELLLTPEAAAALDAPGKTDAAVLIPLYERDGELGTV
                     FTERRHDLSKHAGEISFPGGRQDEPDEDLRRTALREAHEEIGLAAETVELVGALPPVG
                     TFVTGYRVYPFVGLIGPEQAWTLQETEVEKVLEFSLAQLVAGHEMKRLVRKGVPIKTP
                     TYTVDDTLVWGATGRIVQHLLERLAPLLPRPA"
     gene            2949..>3362
                     /locus_tag="VFY44_09230"
     CDS             2949..>3362
                     /locus_tag="VFY44_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910662.1"
                     /translation="MSSRTGQGGLSLGDLVSGLGAVVLLASLWRPWYELRIPEEMIAQ
                     ARALTSRMGEMGPFAQQGLDQLQSQGAVPLTAWEVFEQADTVLAVVAGLTLGLVVLNA
                     VGALAARQDGVLVLAGLVATALVAYKLVNPPGPDVP"
BASE COUNT          516 a         1183 c         1223 g          440 t
ORIGIN      
        1 caccgaggtg cgtgagacga ggagacgctc ggctatctca tcgatggtca ggcgtcggtc
       61 acgcctcagc caccgagctt tctcccgaat gtagggcgga taggtcatgg cgaacgtatg
      121 ttcgctcggg cggcggacga accgcgccac cccggctgca ttagggtccg cgccaatggc
      181 cgaggtcaaa ccgctccacg ccctgcacta cgaccccgag agcgtcgact cgttcgaggc
      241 ggtgatcgcg ccgccctacg acgtgatcga cgccgggctg cgggcgcagc tgctgggcca
      301 gagcccgaag aacgtggtcg agatcgacct gcccgagtcg cccgacggag gcgacatcta
      361 cgagcacgcc gcgcagacgc tggagcagtg gcgcgccgac ggcaccgtgg tgcaggacga
      421 cgagcccgcc tactgggtgc tggaacagga ctacaccgcg ccggacggat cgcagcggcg
      481 gcggcgcggt ttcttcgccc gcgtgcgcgt ggaggactac ggccccgggc gcatccgccc
      541 gcacgagcgc acccaccccg gcccgaagga ggaccggctg cgcctgaccc gggccacgaa
      601 ggccaacctg tcccccatct tcagcctctt ccccgacccc ggcgaccgcg ccgggaaggt
      661 catggaggac gtcgtggccg gcgagccgtt cgcccgtgcc gccgaccacg agggcaccca
      721 gaacgccatg tggcgggtca gcgacccgga cgccgtggcc gagttggagg cggcgctggc
      781 cgaggccgag ctgctgatcg ccgacggcca ccaccgctac gagaccgcgc gcgtgtacgc
      841 ggacgagatc ggcggcgagg gcgaccaccg ctacgtgctg atgtacctgg tctcgctgag
      901 cgacccgggc ctgctggtgt tccccaccca ccggctgctg accggactca aggacgacgc
      961 gctcaagcag gaggccatcc gcgacacgct gacccaggac ttcgacctgg aggagatccc
     1021 ggccgagcag ctggagcccg agcccggcga cgagcgcgtc cgcatgggct acatggactc
     1081 gttccacaag caggcctacg cgctggcgct gaaggaccag tcgatcgccg acaaggcgct
     1141 gtccgacaag ccggccccgt accggcggct ggacaccgcg gtgctggagg cgctggtcct
     1201 caagggcgcc ctgggcatga ccgaggacga catctcgcac ctcaaggggc tgggctactc
     1261 caaggacttc gccgatgcgc gcgacgccgt ggagtcgggc cgcgccgacg cgggcttctt
     1321 catgcgcgcg accccggtgg agcaggtgcg cgaggtggcg gaggccggcg agtcgatgcc
     1381 cccgaagtcg acgtacttct atcccaaggt gccgaccggg ttgctgttca acccgctggc
     1441 ataggcctag catcggccgt ggtgaagatc tatacgaaga agggcgacga cggcacgacc
     1501 ggcctctggt acggcggccg cgtggacaag gactccccgc gccccgaggc ctacgggtcc
     1561 gttgacgagg cggcctcggc gctgggcatg gcccgcgcgg cggccgagcg cgactccgag
     1621 gtctacctgg acatcctgcg catccaggag gagctgttca tcgccgggtc cgagctggcc
     1681 acggcgcctg aggcggccga ccggctggag gccggcgtct cgaagctgac gcaggagatg
     1741 gtggagcgcc tggagcagct gatcgaccgg tacatggacc gggtcgacct gccgccgaag
     1801 ttcgtgatcc ccggcggcac cgagctgtcg gcgcgcctgg acgtggcgcg cacgttcgtg
     1861 cgccgcgccg agcgccgcgt ggagtcgctg aaccgcgccg gcgagctgaa cgacgacacc
     1921 gtcctgacct acctgaaccg ctgctccgac gcggtgtacg cgatcgcccg cttcaccgac
     1981 gaggacagcc ccgagctgtt ccagggcagg gacgcatcga cacggaagga ctcgcacagt
     2041 tgagagcaat cgcacgtcgc tccggcggca ccctgaagca ccacgtcgag atcggcgggc
     2101 accgcctggt ggccgacgag ccctccgagc agggcggaac cgacgccggg ccgagcccgg
     2161 aggagctgct ggcggccagc ctggcctcgt gcagcgcgat cacgatggag gtgcagctgc
     2221 ccgccggcct gcccgaggag cagcgcgaga agctgatgca gatcgtggcc aagtgccccg
     2281 tgcaccgcac cctggagggc gaggtcatgt tcgaggagca ggtctcgctg gtctgacgtg
     2341 ccctcccccg acgatctgcg cgagctgctg ctgacccccg aggcggcggc ggccctggac
     2401 gcccccggca agacggacgc cgcggtgctg ataccgctct acgagcgcga cggcgagctg
     2461 gggacggtgt tcaccgagcg ccggcacgac ctgagcaagc acgcgggcga gatctccttc
     2521 cccggcggcc gccaggacga gcccgacgag gacctgcgcc ggaccgcgct gcgcgaggcg
     2581 cacgaggaga tcgggctggc cgcggagacc gtcgagctgg tcggcgccct gccgcccgtg
     2641 ggcacgttcg tcaccggcta ccgcgtgtac ccgttcgtgg gcctgatcgg gcccgagcag
     2701 gcctggaccc tccaggagac cgaggtcgag aaggtcctgg agttctcgct ggcccagctg
     2761 gtggccggcc acgagatgaa gcgcctggtg cgcaagggcg tgccgatcaa gacccccacg
     2821 tacacggtgg acgacacgct ggtgtggggc gcgaccgggc ggatcgtcca gcacctgctc
     2881 gagcgcctgg cgccgctcct gccccggccg gcctaaggct tccgcgccgg ccggccgatg
     2941 tgcgggacat gagctcccgt accggtcaag gcggcctgtc gctgggcgac ctggtgtcgg
     3001 gtctcggcgc cgtggtgctg ctggcctcgc tctggcgccc ctggtacgag ctccggatac
     3061 cggaggagat gatcgcgcag gcgcgcgccc tgacctcgcg catgggcgag atggggccgt
     3121 tcgcccagca ggggctcgac cagctccagt cccagggcgc ggtcccgctg accgcgtggg
     3181 aggtgttcga gcaggccgac accgtgctgg ccgtggtcgc cggcctgacg ctcgggctgg
     3241 tggtgctgaa cgccgtcggc gccctggccg cgcgccagga cggcgtcctg gtcctggccg
     3301 gcctggtggc caccgcgctg gtggcctaca agctggtgaa cccgcccgga cccgacgtgc
     3361 cg
//
LOCUS       DASUPW010000413         3547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_512353, whole genome shotgun sequence.
ACCESSION   DASUPW010000413 DASUPW010000000
VERSION     DASUPW010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512353"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1309)
                     /locus_tag="VFY44_09235"
     CDS             complement(<1..1309)
                     /locus_tag="VFY44_09235"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HEX5910663.1"
                     /translation="MTPSLTRGAARTVRSLSRRLGRGGGTTLPGRLLLRADPDAMHDM
                     AAELDLGSALISATNGKTTTAAMVAAVLEADGRSLVHNRAGSNMGWGVATALLDARHG
                     RGQTGLFEVDEAWLPAVAEAVQPSAYLLSNLFRDQLDRYGELETLADRWAELVAAEAA
                     EPSRAVRFVLNADDPLVADLGRGHEGAVYFGVGDRSQALPELQHAADSKHCRNCGAPY
                     TYEAVFMGHIGHYRCPNCGRERPAPAVEATTVELHGMSGARVELATPQGSLELNLKLP
                     GLYNVYNAVGAVALALELGVPLTTVGPALDGFGGAFGRVETIAVDGRPVSILLVKNPA
                     GANEVLRTLTLEEGQVDLWLALNDRIADGRDVSWIWDADFELLAGRVRRVTCSGTRAE
                     EMALRLKYAGIDARIEVDRDLGRSLDAAVGDRDGETPLFALPTY"
     gene            complement(1306..2232)
                     /locus_tag="VFY44_09240"
     CDS             complement(1306..2232)
                     /locus_tag="VFY44_09240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX5910664.1"
                     /translation="MKGRRSRFSPPGGRKLAHIRSLLVLCNPTAAGGRAVRRLPEVET
                     ELRRLGADFRSVQTLDAAHARELAAEAGREGKTVVSVGGDGLLRPLAAALRDTDGALA
                     VIPAGRGNDFARVLGVPEDPLVAARIAVQGRERMIDVAQADGEAYLGIASLGFDSDAN
                     RIANESRVIRGNMVYLYSALRALASWKCASFAVTVDGERHELRGYSVAVANSKAYGGG
                     MYIAPDAELDDGLLDVVAIREVSRLRFLANMPKVFRGNHLDEPSVFTHRGSEITVEAE
                     RRFRVYADGDPIADVPVTMTVAQRCLRVVGPA"
     gene            2265..2777
                     /locus_tag="VFY44_09245"
     CDS             2265..2777
                     /locus_tag="VFY44_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910665.1"
                     /translation="MIEPRRIWWARPRKLLAMERPGGGGRSHRPERREGEIAWLVGRR
                     VRCVVSTMATRHNLPAYEAAGLEWHHVPVPVTADGGPALDEILGLLRAELRPAGAVAV
                     HGNRHTDFVAAVCAAHLHEQRGVEPADGLRAAAAAGLTVTPDSCRLLGVDYEALTPTS
                     SASVAAVATG"
     gene            complement(2720..3523)
                     /locus_tag="VFY44_09250"
     CDS             complement(2720..3523)
                     /locus_tag="VFY44_09250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEX5910666.1"
                     /translation="MTALSPLLQSYDQVILDLDGCVRVGDTAVPGSPEAIAALRGAGK
                     RVAFATNNSLHSGEEQVQSLWGIGVQASLADVVTVGGALQHLLAETRTGRGAFVIGTA
                     VLHRHVTDAGLSVLNGTPRAAEAEVVVVGGTDRFDYALLRDAALAARHCGDLLATARD
                     PTLPMPDGPWPGTGSLLAAVEVASGQTAGIVGKPEAQLMLAAMDRLGEGRTLVVGDRL
                     DSDVAAAGAAGLDAALVLSGPDTGEAAEDDPQPVATAATLAELVGVSAS"
BASE COUNT          459 a         1340 c         1326 g          422 t
ORIGIN      
        1 tgtaggtggg cagcgcgaac agcggcgtct cgccgtcacg gtcgccgacc gcggcgtcga
       61 gcgagcgccc gaggtcccgg tcgacctcga tccgagcgtc gatgccggcg tacttcagcc
      121 gcagcgccat ctcctcggcc cgcgtgcccg agcacgtgac ccggcgcacg cggccggcca
      181 ggagctcgaa gtcggcgtcc cagatccagg acacgtcgcg cccgtccgcg atccggtcgt
      241 tcagcgccag ccacaggtcc acctggccct cctccagggt cagggtgcgc aacacctcgt
      301 tcgcgcccgc ggggttcttg accaggagga tcgacaccgg gcggccgtcc acggcgatgg
      361 tctccacgcg gccgaacgcg cccccgaagc cgtccagggc ggggcccacc gtggtcagcg
      421 gcacgcccag ctccagcgcc agggccacgg cgccgacggc gttgtagacg ttgtagagcc
      481 ccggcagctt caggttcaac tcgagcgagc cctggggcgt ggccagctcg acccgggcgc
      541 cgctcatgcc gtgcagctcc accgtggtgg cctcgaccgc gggggcaggg cgctcgcggc
      601 cgcagttggg gcagcggtag tggccgatgt ggcccatgaa cacggcctcg taggtgtagg
      661 gggccccgca gttgcggcag tgcttggagt cggccgcgtg ctggagctcg ggaagcgcct
      721 ggctgcggtc gccgacgccg aagtacaccg cgccctcgtg cccgcggccc aggtcggcca
      781 ccagcgggtc gtccgcgttc aggacgaagc gcaccgcccg ggagggctcg gccgcctcgg
      841 cggccaccag ctcggcccac cggtcggcaa gggtctccag ctcgccgtag cggtccagct
      901 ggtcgcggaa caggttcgac agcaggtacg cgctgggctg caccgcctcg gccaccgccg
      961 gcagccacgc ctcgtcgacc tcgaacagcc cggtctggcc gcggccgtgc cgcgcgtcga
     1021 gcagggcggt ggccacgccc cagcccatgt tcgagcccgc gcggttgtgc accagcgagc
     1081 ggccgtcggc ctccaggacg gccgccacca tcgcggcggt ggtggtcttg ccgttcgtgg
     1141 ccgagatcag cgccgagccc aggtccagct cggcggccat gtcgtgcatg gcgtcggggt
     1201 cggcgcgcag cagcaggcgc ccgggcaggg tggtcccgcc cccgcggccg aggcggcggg
     1261 agagcgagcg caccgtgcgg gcggccccgc gcgtcaggct gggggtcacg ccgggccgac
     1321 cacgcgcagg cagcgctggg ccacggtcat ggtcacgggg acgtcggcga tcgggtcgcc
     1381 gtcggcgtac acgcggaagc ggcgttcggc ctcgaccgtg atctccgagc cgcggtgggt
     1441 gaacacgctg ggctcgtcca ggtggttgcc gcggaacacc ttcggcatgt tggccaggaa
     1501 ccgcaggcgc gagacctccc ggatggccac cacgtccagc aggccgtcgt ccagctcggc
     1561 gtcgggcgcg atgtacatcc cgccgccgta ggccttggag ttggccaccg ccaccgagta
     1621 gccgcgcagc tcgtggcgct cgccgtccac ggtcacggcg aacgaggcgc acttccacga
     1681 tgccagcgcc cgcagggccg agtacaggta gaccatgttg ccccggatca cgcgcgactc
     1741 gttggcgatc cggttggcgt cggagtcgaa gcccaggctg gcgatcccga ggtaggcctc
     1801 gccgtcggcc tgcgccacgt cgatcatccg ctccctgccc tggaccgcga tccgggcggc
     1861 cacgagcggg tcctccggca ccccgagcac ccgcgcgaag tcgttgcccc ggcccgcggg
     1921 gatcacggcg agcgcaccgt cggtgtcgcg cagggcggcc gccagcgggc gcagaagccc
     1981 gtcgccgccc accgagacca ccgtcttgcc ctcccggccc gcctcggcgg ccagctcacg
     2041 cgcgtgggcg gcgtccaggg tctggaccga gcggaagtcc gcgcccaggc ggcgcagctc
     2101 ggtctcgacc tcgggcaggc ggcgcacggc gcgcccgccg gcggccgtcg gattgcagag
     2161 gaccagcagg cttcggatgt gcgccagttt acggccgccg gggggcgaga accggctccg
     2221 ccggcccttc accgggcaga caggcgaccc gaggaggaac ggacatgatc gagccccgca
     2281 ggatctggtg ggcgcgaccc cgcaagctgc tggcgatgga gcggcccggg ggagggggac
     2341 gcagccacag gccggagcgc cgcgagggtg agatcgcgtg gctggtgggc cggcgcgtgc
     2401 gctgcgtggt cagcacgatg gccacccgcc acaacctgcc cgcctacgag gccgccgggc
     2461 tggagtggca ccacgtgccg gtgcccgtga ccgcggacgg cgggcccgcg ctggacgaga
     2521 tcctcggcct gctgcgggcc gagctgcgcc cggccggcgc ggttgccgtg cacggcaacc
     2581 ggcacaccga cttcgtcgcc gccgtctgcg cggcgcacct gcacgagcag cgcggagtcg
     2641 agcccgccga cggcctccgc gccgcggccg ctgcgggcct gacggtcaca cccgactcct
     2701 gccggctgct tggcgtcgac tacgaggcgc tgacgccgac cagctcggcc agcgtggccg
     2761 cggtggccac gggctgaggg tcgtcctcgg ccgcctcgcc cgtgtccggg ccgctgagca
     2821 ccagcgcggc gtccaggccc gccgccccgg cggcggccac gtcggagtcc agccggtcgc
     2881 ccacgaccag cgtccggccc tcgcccaggc ggtccatcgc ggccagcatg agctgggcct
     2941 cgggcttgcc cacgatcccg gccgtctgcc cggacgcgac ctccaccgcg gccagcagcg
     3001 agccggtgcc cggccacggg ccgtccggca tcggcagcgt gggatcgcgg gcggtggcca
     3061 gcaggtcgcc gcagtggcgg gcggccaggg cggcgtcgcg cagcagcgcg tagtcgaaac
     3121 ggtcggtgcc gccgaccacc accacctcgg cctcggcggc gcgcggggtg ccgttcagca
     3181 ccgacagccc cgcgtcggtc acgtgccggt gcagcaccgc ggtgccgatc acgaaggcgc
     3241 cccgcccggt ccgggtctcg gccagcaggt gctgcaacgc gccgcccacg gtgaccacgt
     3301 ccgccaggga cgcctgcacg ccgatgcccc agaggctctg gacctgctcc tcgccggagt
     3361 gcaacgagtt gttggtggcg aaggccacgc gcttgcccgc gccgcgcagg gccgcgatgg
     3421 cctcgggcga gccgggcacc gcggtgtcgc ccacccggac gcagccgtcc agatccagga
     3481 tcacctggtc gtaggactgg agcagagggg agagggcggt cacgtccgca acaacctacg
     3541 gccggcg
//
LOCUS       DASUPW010000414         9370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_374550, whole genome shotgun sequence.
ACCESSION   DASUPW010000414 DASUPW010000000
VERSION     DASUPW010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9370
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374550"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VFY44_09255"
     CDS             <1..420
                     /locus_tag="VFY44_09255"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX5910667.1"
                     /translation="GHPRDLVLENARRKAAAVEGEDVLGADTTVALDGAILGKPADAT
                     EARSHLQRLSGGTHEVWSAVAFRGEAVADVTAVTFRELGPLLDWYVDTGEWEGKAGAY
                     AIQGLGAALVERIDGDYNCVVGLPVAALLALAPDLIG"
     gene            558..1577
                     /locus_tag="VFY44_09260"
     CDS             558..1577
                     /locus_tag="VFY44_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX5910668.1"
                     /translation="MGGRDMAVDLGTANTLVYVRGRGIVLSEPSVVAIDSRTAEVHAV
                     GIEAKRMLGRTPGTIQAIRPLKDGVIADFDVTEQMLRHFIQKVHQNRWAHPRVVVCVP
                     SGVTGVEKRAVEEACLSAGARTADLLEEPMAAAIGAGLPVGEPTGNMVVDIGGGTSEV
                     AVISLGGIVVSQSIRIGGDELDDAIINYVKREYKLMIGQQTAEEVKLEIGSANPLGEE
                     MQAEIRGRDLVSGLPKTVVLTSEEVRLALEEPLQAIIDAVKETLDRTPPELASDIMDR
                     GIMLAGGGALLQGLDERLREETQMPAHLAESPLTCVAVGSGRSLEEFEAIHRANKNSR
                     NQRRR"
     gene            1603..2493
                     /gene="mreC"
                     /locus_tag="VFY44_09265"
     CDS             1603..2493
                     /gene="mreC"
                     /locus_tag="VFY44_09265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX5910669.1"
                     /translation="MFDSKVVRRRRAALAIFIVLSMVMLTASFGESGGPFGALQRGAQ
                     TAFAPLETGAGKALKPFRDLFGWFGDSFDAKDENKKLKAEVEGLREQLGRSATAERDA
                     AQLRGLLKLNRATGFPQATRPVAARVIARSPTVWYSSVKIDAGSDDGIKKNQPVIASG
                     GLAGKVSRVTGGTADVSLITDGTSAVSAQVMPNGANGIVKPEVGKPDDLLLDFVQKKR
                     PVTEGTTVVTSGFKSAEVESLFPRGIPIGRVTRVDAGEVELYQRVHIKPFADLRRVDF
                     VQVLTSRGRPATEQAGVPTP"
     gene            2490..3068
                     /gene="mreD"
                     /locus_tag="VFY44_09270"
     CDS             2490..3068
                     /gene="mreD"
                     /locus_tag="VFY44_09270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX5910670.1"
                     /translation="MRATPGAFLRAGGIALAAVILQLAGLGPMLFFGATPDLVPLVVA
                     AVAIYAGSLSGAAMGFATGFLLDLAVGGTMGVSSLVLTAVGYGVGRYREVRDPAHGLM
                     PIAVGALATLGFELAFAAVSFMLDVGATVSALVFREMLVTVGLNALISLPVFWLCRRI
                     LRPSLAVDPIDKRRRRQGPREAGPIGLRGLEI"
     gene            3077..5002
                     /gene="mrdA"
                     /locus_tag="VFY44_09275"
     CDS             3077..5002
                     /gene="mrdA"
                     /locus_tag="VFY44_09275"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX5910671.1"
                     /translation="MDDDGRRPSLTPQLALRVAVVGGIALVAFGVVFFRLWYLQVLSG
                     DKYRAEANDNRVREIKVQAPRGEIVDRNGKVLVNNRTGLAVRVTPKTLPAKPEDRRDV
                     YRRLGRVLNLSVPRVERRVDRQLKALPYSPATIKQDVPQKTVYYLFENQEDFPGVTVE
                     QEFLREYPHRQVGAQLFGFVREATKEQLDDKQRYRGVGLGDRVGQSGIESQYDRFLRG
                     RNGASRVQVDALGNLRGQLRNRRPEQGRQLRLSLDLGVQEAGQNALGGARGAFAVMNV
                     QDGQVLGLGSSPSFDPNQFSKVLPQSAFKRLTSKESGEPLTNRATSAGYPTGSVFKLI
                     TATAAMEGGLITPDTVLNDGGSLKIGQQVFKNAGDAVHGPVALRKAMSVSSDVFFYLL
                     GQEANGKGNGLLIQDWASKLGLGRRTGIDLPGENPGLIPTPKYRNEGFASFQKCKARK
                     KPTPEQIALGVCGFLDRPWSVGDNVNLSVGQGDLQADPLQMAVAYAAIANGGRVLRPR
                     LGQRIEDATGRPLQELEAPPARKLSIKPEFRQAIMEGLRSAASEPGGTSTPVFKGFKV
                     PIAGKTGTAEKGAGRPDQSWYVALAPYPDPKYVVAVTFEQGGFGADTAAPAARKILGQ
                     LFGVKEEGPIQGGGAPD"
     gene            5034..6233
                     /gene="rodA"
                     /locus_tag="VFY44_09280"
     CDS             5034..6233
                     /gene="rodA"
                     /locus_tag="VFY44_09280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX5910672.1"
                     /translation="MSAAPYGTSLRGFGDARPSAGRILGGLDPALLLATLGLVACSIY
                     TLSTATKSDIPGDPNFFVFRQSLYVAVGILLMLAISRVDYSRLREWKAGLYGALIGLI
                     VLVYVFGGVTRGSKRAIQLPFFEIQTSELGKILLIVTLSAFVVDRMRRMGDRETTSRI
                     MLLALFPAMLVIAQPDLGSGLVYISIVLAILFVAGTKWTHFAALGSLAATAVVIVLVA
                     APALGVELLKPYQVDRLTSFINPAEDPAEEGYQQQQSLVAVGAGGKTGRGDEATQTRL
                     DFLPEHHTDFIFSVVGEEFGFMGAALVLSLYALLIWRCLRILTMSKNLYGTLIAAGIT
                     AMFMHQVFINVGMAIGIMPITGVTLPLLSYGGSSVLVTFLALGLLQSIHAQARETAFA
                     KGRDMAL"
     gene            6252..8192
                     /locus_tag="VFY44_09285"
     CDS             6252..8192
                     /locus_tag="VFY44_09285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX5910673.1"
                     /translation="MRKQVLVTADRGETRVAILEAEGTPPAEAGNNSKNRSGNKGGND
                     NGNDANWRVGELYIERRGSRSIVGNVYKGKVDNVLPGLEAAFVDIGLEKNGFLHADDV
                     VIPGVEVARRGRTGGKGKRISELLKPGQEILVQAIKDPLKTKGPRLSMQLSIAGRYLV
                     YVPQGEGVGVSRRLEDKERDRLRKAAKGLELGDGGAIIRTAAQGAKKADFEREIKYLH
                     KLHDVLTQRSEEATAPAMVFQEADLSVRVVRDIFSKQFERAIVDDPGQHQRLQSFFSR
                     TAPELLERLDLYEDPKPSLFERYGIDREIRSTLRKRVDLPSGGYLIIDYAEAGTVIDI
                     NSGSFTGRGKGARLEDTITKTNLEAAEEVVRQLRLRDIGGIIVIDFIDMSRAKNRDAV
                     LKVLRKALDEDRTKTYVVEISPLGLVEMTRQNVTEGVREILTKTCPTCEGEGVVLSEE
                     TVALDVERRLRELAGERPDTEAFLIRAHSRVIELVAGGPGRPLLEIEEETGKYFHFEG
                     SEGVPVDHFSVVLEGSNEEVQERALPFSEGDEVLVTIEEPHMYAEDDAVAKFDGYVIS
                     VAGAGKLVGKKTLVRIEDVGRSAASAVLVDGADVVDAPVVADSTLGGAGGPANGATPK
                     PRRRGRRGGRGRRRKESESAND"
     gene            8203..8556
                     /locus_tag="VFY44_09290"
     CDS             8203..8556
                     /locus_tag="VFY44_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910674.1"
                     /translation="MSSPRTIATTVLAALALGLVACGGESDEDQVKEVANQLANTDEK
                     VCDNATDKFLKAVGGTKEQCKKSAREDDGSTKPKVGKITVDGDKATAVLSADGSKATF
                     GLVKDGDQWKLDSVR"
     gene            8574..8891
                     /gene="rplU"
                     /locus_tag="VFY44_09295"
     CDS             8574..8891
                     /gene="rplU"
                     /locus_tag="VFY44_09295"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX5910675.1"
                     /translation="MYAIVKVGGKQYRVQTGDSLLVDRMPGDEGAKVTLEPLMFRGDK
                     DDDSVLSGDGLSKVKVEATITGHERGKKIHGLKFKPKRGYKVRYGARADLTRIEIGAI
                     KNG"
     gene            8903..9154
                     /gene="rpmA"
                     /locus_tag="VFY44_09300"
     CDS             8903..9154
                     /gene="rpmA"
                     /locus_tag="VFY44_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX5910676.1"
                     /translation="MAHKKGLGSSRNGRDSNAQRLGVKVFAGQAVTGGEIIVRQRGTR
                     FRPGEGVGIGKDDTLFAARAGTVQFQTGHRGRTVTVDPS"
BASE COUNT         1487 a         3277 c         3290 g         1316 t
ORIGIN      
        1 ggccatccgc gcgacctggt cctggagaac gcgcgccgca aggcggccgc cgtggagggc
       61 gaggacgtcc tgggcgcgga caccacggtg gccctggacg gggcgatcct gggcaagccg
      121 gccgacgcca ccgaggcccg cagccacctg caacggctct cgggcggcac gcacgaggtg
      181 tggtcggccg tcgccttccg cggggaggcg gtggccgacg tgaccgcggt gaccttccgc
      241 gagctcggcc cgctgctgga ctggtacgtg gacacggggg agtgggaggg caaggcgggc
      301 gcctacgcca tccagggcct cggcgcggcg ctcgtggagc ggatcgacgg cgattacaac
      361 tgcgtggtcg gcctgcccgt ggccgcgctc ctggcgctgg cccccgacct gatcgggtag
      421 gcggcggcac gccttgcaat cccccttgac gggggccttc agccgctaca ctggcgtcct
      481 tcgggagcgc cctgcggaac ggtccgccgg cggcccggat acctccaccc atgggattct
      541 tctcttacct cacaggcatg ggcggccgag acatggccgt cgacctcggc accgccaaca
      601 cgctggtgta cgtgcggggc cgcgggatcg tgctctcgga gccgtccgtg gtggccatcg
      661 actcgcgcac cgcagaggtg cacgcggtgg gcatcgaggc caagcggatg ctgggccgca
      721 cgcccgggac catccaggcc atccggccgc tgaaggacgg cgtgatcgcc gacttcgacg
      781 tcacggagca gatgctgcgc cacttcatcc agaaggtgca ccagaaccgc tgggcgcacc
      841 cgcgcgtcgt ggtctgcgtg ccttcgggcg tgaccggggt cgagaagcgc gccgtcgagg
      901 aggcctgcct gtcggcgggc gcccgcacgg cggacctgct cgaggagccg atggctgccg
      961 cgatcggggc cggcctcccg gtcggcgagc ccaccgggaa catggtggtg gacatcggcg
     1021 gtggcaccag cgaggtcgcg gtgatctcgc tgggcggcat cgtggtgtcc cagtcgatcc
     1081 gcatcggcgg cgacgagctg gacgacgcga tcatcaacta cgtcaagcgc gagtacaagc
     1141 tgatgatcgg tcagcagacg gcggaggagg tcaagctgga gatcggctcg gccaacccgc
     1201 tgggcgagga gatgcaggcc gagatccgcg gccgcgatct ggtctccggt ctgcccaaga
     1261 cggtcgtgct gaccagcgaa gaggtgcgcc tggctctcga ggagccgctg caggccatca
     1321 tcgacgccgt caaggagacc ctggaccgca caccgccgga gctggcctcg gacatcatgg
     1381 accggggcat catgctggcc ggcgggggag ccctcctaca ggggctggac gagcgcctgc
     1441 gtgaggagac gcagatgccc gcccacctgg cggagtcccc gctcacgtgc gtcgcagtcg
     1501 ggtccggccg cagcctcgag gagttcgagg caattcaccg cgccaacaag aattcccgaa
     1561 accagcggcg tcgttagccc ggcgccgcga aggcggcact gaatgttcga cagcaaagtg
     1621 gtccgcaggc gaagggcggc cctggcgatc ttcatcgttc tctcgatggt catgctcacc
     1681 gcatcgttcg gtgagtccgg cggccccttc ggcgccctcc agcgcggcgc ccagaccgcg
     1741 ttcgcgcccc tcgagaccgg tgccggcaag gcgctgaagc cgttccgcga cctgttcggc
     1801 tggttcggcg acagcttcga cgccaaggac gagaacaaga agctcaaggc cgaggtcgag
     1861 ggcctgcgcg agcagctcgg ccggtcggcc accgccgagc gcgacgccgc ccagctgcgc
     1921 ggcctgctca agctcaaccg cgccaccggc ttcccgcagg ccacccggcc cgtggccgca
     1981 cgcgtgatcg cccgctcgcc gaccgtctgg tactcgtcgg tcaagatcga cgcgggctcc
     2041 gacgacggca tcaagaagaa ccagccggtg atcgcctcgg gcgggctggc cggcaaggtc
     2101 agccgcgtga ccggcggcac cgccgacgtg tcgctgatca ccgacggcac cagcgcggtg
     2161 tcggcccagg tcatgcccaa cggcgccaac ggcatcgtca agcccgaggt gggcaagccc
     2221 gacgacctgc tgctggactt cgtgcagaag aagcgcccgg tcaccgaggg caccaccgtc
     2281 gtgacctccg gcttcaagtc ggccgaggtg gagtcgctgt tcccgcgcgg aatcccgatc
     2341 ggccgcgtga cacgcgtcga cgccggcgag gtcgagctct accagcgcgt gcacatcaag
     2401 cccttcgccg acctgcgccg cgtggacttc gtccaggtgc tgacctcgcg cggccgtccc
     2461 gccaccgagc aggccggggt gccgacgccg tgagggccac ccccggagcc ttcctgcgcg
     2521 cgggcggcat cgccctggcc gccgtgatcc tgcagctggc gggcctcggg cccatgctct
     2581 tcttcggcgc cacccccgac ctggtcccgc tggtcgtcgc cgccgtggcc atctacgccg
     2641 gcagcctgtc cggcgcggcc atgggcttcg ccaccggctt cctgctggac ctggccgtcg
     2701 gcggcaccat gggggtctcg tcgctggtgc tgaccgccgt cggctacggc gtggggcgct
     2761 accgcgaggt gcgcgacccc gcccacgggc tgatgccgat cgccgtcggc gccctggcca
     2821 cgctgggctt cgagctggcc ttcgccgcgg tgtcgttcat gctcgacgtg ggcgccaccg
     2881 tcagcgcgct ggtcttccgc gagatgctgg tcacggtggg cctgaacgcc ctgatctcgc
     2941 tgcccgtgtt ctggctctgc cgccgcatcc tgcgcccgtc gctggccgtg gacccgatcg
     3001 acaagcgccg ccgccgccag ggcccccgtg aggcgggccc gatcggcctg cgcgggttgg
     3061 agatctgacg atgtacatgg acgacgacgg ccgccggccc tcgctgacgc cccagctcgc
     3121 gctgcgggtg gcggtggtgg gcgggatcgc gctcgtggcc ttcggcgtcg tgttcttccg
     3181 cctctggtac ctgcaggtgc tgtcgggcga caagtaccgc gccgaggcca acgacaaccg
     3241 ggtgcgcgag atcaaggtgc aggcgccgcg cggcgagatc gtggaccgca acggcaaggt
     3301 gctggtgaac aaccgcaccg gcctggcggt gcgcgtcacg ccgaagacgc tgccggccaa
     3361 gcccgaggac cgccgcgacg tctaccgccg cctgggccgg gtgctcaacc tgtcggtgcc
     3421 gcgggtcgag cggcgggtgg accgccagct caaggcgctg ccgtactcgc cggccacgat
     3481 caagcaggac gtcccgcaga agaccgtcta ctacctgttc gagaaccagg aggacttccc
     3541 cggggtcacc gtggagcagg agttcctgcg cgagtacccg caccggcagg tcggcgcgca
     3601 gctgttcggc ttcgtgcgcg aggccaccaa ggagcagctg gacgacaagc agcgctaccg
     3661 cggggtcggg ctgggcgacc gtgtcggcca gtccgggatc gaatcccagt acgaccgctt
     3721 cctgcgcggc cgcaacggcg ccagccgggt ccaggtggac gcgctgggca acctgcgcgg
     3781 ccagctgcgc aaccgccggc ccgagcaggg ccgccagctg cgcctgtcgc tggacctcgg
     3841 cgtccaggag gcgggccaga acgccctggg cggcgcccgc ggcgcgttcg cggtcatgaa
     3901 cgtccaggac ggccaggtgc tgggcctggg cagctccccg agcttcgacc ccaaccagtt
     3961 ctccaaggtg ctgccccagt cggccttcaa gcgcctgacc tcgaaggaga gcggcgagcc
     4021 gctgaccaac cgcgccacct cggccggcta tcccaccggc tcggtgttca agctcatcac
     4081 cgccaccgcg gcgatggagg gcgggctgat cacccccgac accgtgctca acgacggcgg
     4141 ctcgctgaag atcggccagc aggtgttcaa gaacgccggc gacgcggtgc acggccccgt
     4201 ggcgctgcgc aaggccatgt cggtgtccag cgacgtgttc ttctacctgc tggggcagga
     4261 ggccaacggc aagggcaacg ggctgctgat ccaggactgg gccagcaagc tcgggctggg
     4321 ccggcggacc ggcatcgacc tgccgggcga gaacccgggc ctgatcccca cgcccaagta
     4381 ccgcaacgag ggcttcgcca gcttccagaa gtgcaaggcg cgcaagaagc ccacgccgga
     4441 gcagatcgcg ctgggcgtgt gcggcttcct ggaccgcccg tggagcgtcg gcgacaacgt
     4501 gaacctgtcc gtcggccagg gcgacctgca ggccgacccg ctccagatgg ccgtggccta
     4561 tgcggcgatc gccaacggcg gccgcgtcct gcgcccgcgc ctggggcagc ggatcgagga
     4621 cgccaccggg cgcccgctgc aggagctgga ggcgccgccg gcgcgcaagc tgagcatcaa
     4681 gcccgagttc cgccaggcga tcatggaggg gctgcgcagc gcggccagcg agcccggtgg
     4741 cacgtcgacc ccggtgttca agggcttcaa ggtccccatc gccggcaaga cgggcaccgc
     4801 cgagaagggc gccggccgtc ccgaccagtc gtggtacgtg gccctggcgc cctaccccga
     4861 ccccaagtac gtggtggccg tgaccttcga gcagggcggg ttcggcgcgg acaccgcggc
     4921 ccccgcggcg cgcaagatcc tgggccagct gttcggcgtc aaggaagagg gcccgatcca
     4981 gggcgggggc gccccggact gatggcccct gcgaccaata ggctggtccg cgcatgagcg
     5041 ccgctcccta cggcacctcc ctccgggggt tcggggacgc ccgacccagt gcgggacgga
     5101 tcctgggcgg cctggacccg gcgctcctgc tggccacgct gggcctggtg gcgtgcagca
     5161 tctacacgct gtcgaccgcc accaagtcgg acatccccgg cgaccccaac ttcttcgtct
     5221 tccgccagtc gctgtacgtg gccgtcggca tcctgctgat gctggccatc tcacgggtcg
     5281 actactcccg cctgcgggag tggaaggccg gcctctacgg cgcgctcatc gggctgatcg
     5341 tgctcgtcta cgtcttcggc ggcgtcacgc gcggctccaa gcgcgccatc cagcttccgt
     5401 tcttcgagat ccagacctcc gagctgggca agatcctgct catcgtcacg ctttcggcct
     5461 tcgtggtcga tcggatgcgc aggatgggag accgcgagac caccagcagg atcatgctgc
     5521 tggcgttgtt cccggcgatg ctcgtcatcg cccagcccga cctcgggtcg ggcctcgtgt
     5581 acatcagcat cgtgctggcc atcctgttcg tggccggcac caagtggacc catttcgcag
     5641 cgctgggcag cttggccgcc accgccgtcg tgatcgtcct cgtggcggcg ccggcgctgg
     5701 gggtcgaact gctcaagccg taccaggtgg atcgcctcac gtcgttcatc aacccggccg
     5761 aggatccggc ggaggagggc taccagcagc agcagtcgct ggtggccgtc ggcgccggtg
     5821 gaaagaccgg tcgtggcgac gaggcgacgc agaccaggct ggacttccta cccgagcacc
     5881 acaccgattt catcttcagt gtggtgggcg aggagttcgg attcatgggg gcggcactag
     5941 tcttgtccct atacgcctta ctgatctgga gatgcctgcg aatcctgacg atgtcgaaga
     6001 acctgtacgg gactttgatc gccgccggga taaccgccat gttcatgcac caggtcttca
     6061 tcaacgtagg catggccatc gggatcatgc ccatcaccgg agtcaccctg ccgctgctca
     6121 gctacggcgg gtcctccgtg ctcgtcacct tcctggctct tggccttctt cagtcgatcc
     6181 acgcgcaggc gcgcgagacg gctttcgcca agggccgcga catggctcta tgagctttat
     6241 gagaggaatt tttgagaaag caagtactcg tcacggcgga ccgtggcgaa acacgcgtcg
     6301 ccatcctcga ggcggagggc acgccccccg ctgaggcggg caacaacagc aagaaccgct
     6361 ccggcaacaa gggcggcaac gacaacggca acgacgccaa ctggcgtgtg ggcgagctct
     6421 acatcgagcg gcgcggatcg cgctcgatcg ttggcaacgt ctacaagggc aaggtcgaca
     6481 acgtcctgcc cgggctcgag gccgcgttcg tggacatcgg gctggagaag aacggcttcc
     6541 tgcacgccga cgacgtggtc atccccggcg tcgaggtggc ccgccgcggt cgcaccggcg
     6601 gcaagggcaa gcgcatctcc gagctgctga agccgggcca ggagatcctg gtccaggcca
     6661 tcaaggaccc gctcaagacc aaggggccgc gcctgtcgat gcagctgtcg atcgccggcc
     6721 gctacctggt ctacgtgccc cagggcgaag gcgtgggcgt gtcccggcgc ctcgaggaca
     6781 aggagcgcga ccggctgcgc aaggccgcca agggcctgga gctgggcgac ggcggagcca
     6841 tcatccgcac cgccgcccag ggcgcgaaga aggccgactt cgagcgcgag atcaagtacc
     6901 tgcacaagct ccacgacgtg ctgacccagc gctcggagga ggccaccgct ccggccatgg
     6961 tcttccagga ggccgacctg tcggtgcgcg tagtgcgcga catcttctcc aagcagttcg
     7021 agcgcgcgat cgtcgacgac ccgggccagc accagcggct gcagtccttc ttcagccgca
     7081 ccgcccccga gctgctcgag cgcctggacc tgtacgagga ccccaagccc tcgctgttcg
     7141 agcgctacgg catcgaccgc gagatccggt ccacgctgcg caagcgggtg gacctgccct
     7201 cgggcggcta cctgatcatc gactacgccg aggcgggcac ggtgatcgac atcaactcgg
     7261 gctcgttcac cggccgcggc aagggcgccc gcctcgagga caccatcacc aagaccaacc
     7321 tcgaggccgc cgaggaggtg gtgcgccagc tccggctgcg cgacatcggc gggatcatcg
     7381 tcattgactt catcgacatg agccgcgcca agaaccgcga cgcggtgctc aaggtgctgc
     7441 gcaaggcgct ggacgaggac cgcaccaaga cctacgtggt cgagatctcg ccgctgggcc
     7501 tggtggagat gacgcgccag aacgtgaccg agggcgtgcg cgagatcctg accaagacct
     7561 gccccacctg cgagggcgag ggcgtggtcc tgtcggagga gaccgtcgcg ctggacgtgg
     7621 agcggcgcct gcgcgagctg gccggcgagc ggcccgacac cgaggccttc ctgatccggg
     7681 cgcactcgcg cgtgatcgag ctggtggccg gcggccccgg ccgcccgctg ctggagatcg
     7741 aggaggagac cggcaagtac ttccacttcg agggatccga gggcgtgccc gtggatcact
     7801 tctcggtggt cctggagggc tcgaatgagg aggtccagga gcgcgcgctg cccttcagcg
     7861 agggcgacga ggtgctggtg accatcgagg agccgcacat gtacgccgag gacgacgccg
     7921 tcgccaagtt cgacggctac gtgatctccg tcgcgggcgc gggcaagctg gtgggcaaga
     7981 agacgctggt gcggatcgag gacgtcggcc gctccgccgc ctcggccgtg ctggtggacg
     8041 gcgccgacgt ggtggacgcc cccgtggtgg ccgacagcac cctcggaggc gcgggcggcc
     8101 ccgcgaacgg cgcgaccccg aagccccgcc gtcgcggccg ccgtggcggc cgcgggcgcc
     8161 ggcgcaagga gtcggagagc gccaatgact agggtccgct cgatgagctc cccgcgcacc
     8221 atcgccacca ccgtcctggc cgctctggcc ctcggcctcg tcgcctgcgg cggcgagtcc
     8281 gacgaggacc aggtcaagga ggtggccaac cagctggcca acaccgacga gaaggtgtgc
     8341 gacaacgcca ccgacaagtt cctgaaggcc gtcggcggca ccaaggagca gtgcaagaag
     8401 tcggcgcgcg aggacgacgg ctcgaccaag cccaaggtcg gcaagatcac cgtggacggc
     8461 gacaaggcca ccgccgtgct cagcgccgac ggcagcaagg ccaccttcgg cctcgtcaag
     8521 gacggggacc agtggaagct ggattcggtt cgctaagctc cccggtcgct ttcatgtacg
     8581 caatcgtcaa agtcggtggc aagcagtacc gcgtccagac gggggactcc ctgctggtcg
     8641 accgcatgcc cggggacgag ggggccaagg tcaccctcga gccgctcatg ttccggggcg
     8701 acaaggacga cgactcggtg ctctccggcg acggcctgag caaggtcaag gtcgaggcca
     8761 ccatcaccgg ccacgagcgc ggcaagaaga tccacgggct caagttcaag cccaagcgcg
     8821 gctacaaggt ccgctacggc gcccgcgccg acctcacgcg catcgaaatc ggcgccatca
     8881 agaacggcta ggagggacag caatggctca taagaagggg ctcggctcct cccgcaacgg
     8941 ccgcgactcc aacgcgcagc gcctgggcgt caaggtgttc gccggccagg ccgtcaccgg
     9001 cggcgagatc atcgtccgtc agcgcggcac ccgcttccgc cccggcgagg gcgtaggcat
     9061 cggcaaggac gacacgctgt tcgccgcccg cgcgggcacg gtgcagttcc agaccggcca
     9121 ccgcggccgc accgtcaccg tcgacccttc gtagagccgt cccccgaatg ggggagcgcg
     9181 gaatccgctg attgccctta ggttgggcgc ggcacggacc gcatctcacc tttcggaggc
     9241 aatatgaaga gcaggactgg cgcaacggcg ctggctctcg ccgctgcgct gctggtcggc
     9301 gcctgtggcg gggacgacga ggagcagaag aagaaggacc aggcggctgc cgccgccgcg
     9361 gccaagaacc
//
LOCUS       DASUPW010000415         5694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1897488, whole genome shotgun sequence.
ACCESSION   DASUPW010000415 DASUPW010000000
VERSION     DASUPW010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1897488"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VFY44_09305"
     CDS             complement(<1..731)
                     /locus_tag="VFY44_09305"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5910677.1"
                     /translation="MPARAFVGVVLTLIVAFAAGCGGGDSGSGSRTINWYVFSEPGGA
                     YDAAVTTCNKQAKGAYKINLERLPTDANAQRELLVRRLAAEDSSIDLMGMDVIWTAEF
                     AQANWIKEWTGADEAAATKGKLKGPLRSVQYQDKTWAMPFTSNTQLLWYRKDKVKTPP
                     KTWDEMIDQAIKLNTAIEVQGRQYEGLTVWVNSLIASAGGQIVDENGKVKVDATAKQA
                     AEIMKRLASSKAAPPGLSTNAEDQAR"
     gene            complement(780..2210)
                     /locus_tag="VFY44_09310"
     CDS             complement(780..2210)
                     /locus_tag="VFY44_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX5910678.1"
                     /translation="MSWIERAVIYQVYPRSFQDASGDGIGDLAGIERRLGHIADLGAA
                     ALWLSPVYPSPGVDMGYDIADFRDVDPLFGDLASLDRLVASAHDQGLRVLLDLVPCHT
                     SIHHPWFTEHPEYYVWADGDRPPNNWVANFGGPAWTLDPERGRWYLHSYYPEMPDLDW
                     RNPDVVAAMQDVVRFWLDRGVDGFRLDAIQGLMKDPAMRDDPPAERPFGLPLPAEYGR
                     LEHVHSTNSPDVGEAVAALRAAAGDAPLVGEVYLPAADRGPYLEHLDVAFAFELLHAP
                     WDAAALAAAIEANAGGGAAWVLSNHDFPRVPTRVGEANARAAAVLLLTLPGPAFVYQG
                     EELGQADAPGPGTRYDRHGRDSCRHPVQWEPDPRTGGFTDGEPWLAPVDAPRRNVRDQ
                     TGDPGSMLELYRALIALRADLDGDVSLLEAADGVVAFRRGRHTVAVNTSSAPAPMPAR
                     GPAVVSTHPGEGDAATLVSHEARVVG"
     gene            2335..3126
                     /locus_tag="VFY44_09315"
     CDS             2335..3126
                     /locus_tag="VFY44_09315"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5910679.1"
                     /translation="MIDIEIGIVRPELTLQMPADQDTLPLVRQALRSLGETVDADLEA
                     LEDAELAVTEACANAVEHAYADGKGDIEVRFVPGEAELQVSVADHGVGIPVDVRTNRV
                     GDGFGLPMIDSIATSVDFADGDGTHITMALPLGRPEPPVAVDGALPGVEPAERILRRL
                     VAVVAAQRDMSSERVMEGLLLAELVARNSLRYLIGDTVSVHIDLVDQGFELRVGPLEP
                     GGAQAVIDDSEVPVVGSVLERLADEVRVDHADDGESITLRINPRG"
     gene            complement(3130..3462)
                     /locus_tag="VFY44_09320"
     CDS             complement(3130..3462)
                     /locus_tag="VFY44_09320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5910680.1"
                     /translation="MSLFEVDITEGRLGPVAALSGEVDLAAAEAVEERIRTTLTDEPD
                     VLVLDLRAVEFLDSSGLRVVLSLDKAQRDRGARLVVVRGGRRVARVMELTGADRQLEM
                     VDDPAEIQ"
     gene            3617..4300
                     /locus_tag="VFY44_09325"
     CDS             3617..4300
                     /locus_tag="VFY44_09325"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5910681.1"
                     /translation="MQDEARKDELDVEEPSSRSQFLKIAGGTGAAGALALFIAACGGD
                     DEKSSSSSSSSSDKEKMGSGSAKGDLKIVNYALTLEYLEADFYKQVIESGEIKDKKLG
                     EVAKTIANDENEHVAALTATVEQLGGKPAAKPTTNFDDVLAGGEKKILETAATVENLG
                     AAAYLGQAANIKSKEVLAAALSIHTVEGRHAAVLNSVIGKSIVPDGAFAKPADMATVL
                     KAVKPFIKA"
     gene            4331..4966
                     /locus_tag="VFY44_09330"
     CDS             4331..4966
                     /locus_tag="VFY44_09330"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5910682.1"
                     /translation="MSDKNMKAPELANIEVQGLTRESFIMRGAIAAGSVYGLSTVGPF
                     VSQALAQGDGDVDILNFALTLEYLEAAFYTEALKQVGGLRAEVKELATTTRDDENEHV
                     DAIAATVKKLGGKPAAKPGVDFGKAFASEKTFLTTAVVFEDLGVGAYNGAGPSIKSKE
                     VLAAAGSIVQVEGRHAGAVRLLNGDKIAPSPFDKGIPMAEVLMKAKPFIKS"
     gene            complement(5020..>5694)
                     /locus_tag="VFY44_09335"
     CDS             complement(5020..>5694)
                     /locus_tag="VFY44_09335"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX5910683.1"
                     /translation="LQDTIEGLTQDRGAAGVLLVLGILGALNAASGYIGAFIRASNVI
                     YEVQEGRPFYKLRPLQLAVTITIVGAVGLVITALVLSGPLAGAVGDALGLSEEAVTVY
                     GVAKWPVLALLVMGILGLLYYASPNARVTGIRWVTPGSLLALVVWVGASALFALYVAN
                     FGSYNKTYGTLGGAVTFLVWLWITNIAVLLGAELNAELERSREIKAGVSGAEEEIQLP
                     PREEAG"
BASE COUNT          893 a         2006 c         2004 g          791 t
ORIGIN      
        1 cgggcctggt cctccgcgtt ggtggacagg ccgggcggcg ccgccttcga gctggccagg
       61 cgcttcatga tctcggccgc ctgcttggcg gtggcgtcca ccttgacctt gccgttctcg
      121 tccacgatct ggcccccggc ggatgcgatc agggagttga cccacacggt cagcccctcg
      181 tactggcggc cctggacctc gatcgccgtg ttcagcttga tcgcctggtc gatcatctcg
      241 tcccaggtct tgggcggcgt cttgaccttg tccttgcggt accagagcag ctgggtgttg
      301 ctggtgaagg gcatcgccca ggtcttgtcc tggtactgga cgctgcgcag cggccccttg
      361 agcttgccct tggtggcggc ggcctcgtcg gcgccggtcc actccttgat ccagttggcc
      421 tgggcgaact cggcggtcca gatcacgtcc atgcccatca ggtcgatgct cgagtcctcg
      481 gcggccagcc gccgcaccag cagctcgcgc tgggcgttgg cgtcggtggg caggcgctcg
      541 aggttgatct tgtacgcgcc cttggcctgc ttgttgcagg tggtcacggc ggcgtcgtag
      601 gcgccgccgg gctcgctgaa cacgtaccag ttgatggtcc gcgaacccga gccgctgtca
      661 ccgccaccgc aacctgcggc gaacgcgacg atcagggtca gtaccactcc tacgaatgcc
      721 cgggcgggca tgtgcgtctc ctctcctcgc cggagtccct accccccggc agggtgaaac
      781 taacccacca cacgagcctc gtgcgaaacc agggttgcgg cgtcgccctc cccggggtgg
      841 gtggacacca ccgccgggcc ccgggcgggc atgggcgcgg gcgcggagga ggtgttcacg
      901 gccaccgtgt ggcgcccgcg gcggaaggcc acgacgccgt cggcggcctc gagcagcgac
      961 acgtcgccgt ccaggtcggc gcgcagcgcg atcagggccc ggtacagctc gagcatggag
     1021 ccggggtcac cggtctggtc gcgcacgttg cgccggggcg cgtccaccgg cgccagccac
     1081 ggctcgccgt cggtgaaccc gccggtgcgc gggtcgggct cccactgcac cgggtggcgg
     1141 cagctgtcgc ggccgtgacg gtcgtagcgc gtgccgggcc cgggcgcatc ggcctggccc
     1201 agctcctcgc cctggtagac gaacgcgggg cccggcaggg tcagcagcag cacggcggcc
     1261 gcgcgggcgt tcgcctctcc cacgcgcgtg ggcacgcgcg ggaagtcatg gttggacagc
     1321 acccaggccg cgccgccgcc cgcgttggcc tcgatcgccg cggccagcgc ggccgcgtcc
     1381 cagggggcgt gcagcagctc gaacgcgaac gccacgtcca ggtgctcgag gtacggcccc
     1441 cggtccgcgg cgggcaggta cacctcgccc accagggggg cgtccccggc ggccgcccgg
     1501 agcgcggcca cggcctcgcc cacgtccggg ctgttggtgg agtgcacgtg ctccagccgc
     1561 ccgtactccg cgggcagcgg gaggccgaac ggccgctcgg ccggcgggtc gtcgcgcatc
     1621 gcggggtcct tcatcaggcc ctggatcgcg tccaggcgga agccgtcgac cccgcggtcc
     1681 agccagaacc gcaccacgtc ctgcatcgcg gccaccacgt ccgggttgcg ccagtccagg
     1741 tcgggcatct cggggtagta gctgtgcagg taccagcgcc cgcgctccgg atccagggtc
     1801 caggccgggc cgccgaagtt ggccacccag ttgttcggag gccggtcgcc gtcggcccac
     1861 acgtagtact ccgggtgctc ggtgaaccac gggtggtgga tcgacgtgtg gcacgggacc
     1921 aggtccagca gcacgcggag gccctggtcg tgggccgacg cgaccagccg gtccagggag
     1981 gccagatcgc cgaacagcgg gtcgacgtcg cggaagtcgg cgatgtcgta gcccatgtcc
     2041 acgccgggcg aggggtacac gggcgagagc cacagcgcgg cggcgcccag atccgcgatg
     2101 tggcccaggc gccgctcgat gccggccagg tccccgattc cgtcgccgct ggcgtcctgg
     2161 aacgatcgcg ggtagacctg gtagatcacg gctcgctcga tccagctcac ggcggcggag
     2221 actagttcgc ttgcgccgaa ccgttttcgg cttgtgggct atgggtagtc gcgcgtcaat
     2281 gagaatgtcc gcccggcccg gccggtacgg gcatcaccag acacggagtc acgcgtgatc
     2341 gacatcgaga tcggcatcgt gcggccggag ctgacgctcc agatgcccgc cgaccaggac
     2401 acgctgcctc tggtgcggca agctctccga tccctcggcg agaccgtcga tgccgacctc
     2461 gaagcgctcg aggacgccga gctggcggtg accgaggcgt gcgccaacgc cgtcgagcac
     2521 gcctacgccg acggcaaggg cgacatcgag gtgcggttcg tcccgggcga ggccgagcta
     2581 caggtcagcg tggccgacca cggcgtcggc atcccggtgg acgtacgtac gaaccgggtg
     2641 ggcgacgggt tcggcctgcc gatgatcgac agcatcgcca ccagcgtcga cttcgccgac
     2701 ggcgacggca cccacatcac catggcgctc ccgctgggcc ggcccgagcc gcccgtggcg
     2761 gtcgacggcg ccctgccggg cgtcgagccc gccgagcgga tcctgcgccg gcttgtggcc
     2821 gtggtggccg cccagcgcga catgtccagc gagcgcgtca tggagggcct gctcctggcc
     2881 gagctggtgg cccggaactc gctgcgctac ctgatcggcg acacggtctc ggtgcacatc
     2941 gacctggtcg accagggctt cgagctgcgc gtgggcccgc tggagcccgg tggggcccag
     3001 gccgtgatcg acgacagcga ggtgcccgtg gtgggctccg tcctggagcg cctggccgac
     3061 gaggtgcgcg tggaccacgc cgacgacggc gagagcatca cgctgcgaat caacccccgc
     3121 ggctgagcgc tactggatct cggcggggtc gtcgaccatc tcgagctggc ggtcggctcc
     3181 cgtcagctcc atcacgcgcg cgacccggcg gccgccgcgc acgaccacca ggcgggcgcc
     3241 ccggtcgcgc tgggccttgt ccagcgacag caccacgcgc agaccgctgg agtccaggaa
     3301 ctcgaccgcg cgcaggtcca gcaccaggac gtcgggctcg tcggtgagcg tggtacgaat
     3361 tcgttcctcg accgcctcgg cggcagccag gtccacctcg ccggaaagtg cggcgacggg
     3421 gcccagtcga ccttccgtga tgtccacttc gaacaggctc accgggcaac ggcgcgtata
     3481 taccacgcgc ggtgtgaccg gtgcgcacgc gccaggccgc tgatccggca gtcaacgggc
     3541 cacgcaggaa cagcagacac cggaggcgcg ccccgtgcgg gccccctagc cgaaccccga
     3601 cgaagggacc caaacgatgc aggacgaagc ccgaaaggac gagctggacg tggaggagcc
     3661 ctcctcccgt tcccagttcc tcaagatcgc cggcggcacc ggcgcggccg gcgcgctggc
     3721 cctgttcatc gccgcctgcg gcggtgacga cgagaagagc tcttcgagct ccagctccag
     3781 ctccgacaag gagaagatgg gcagcggctc cgcgaagggc gatctcaaga tcgtcaacta
     3841 cgcgctgacg ctggagtacc tcgaggccga cttctacaag caggtcatcg agtccggcga
     3901 gatcaaggac aagaagctcg gagaggtcgc caagaccatc gccaacgacg agaacgagca
     3961 cgtcgcggcg ctgacggcca cggtcgagca gctgggtggc aagcctgccg ccaagcccac
     4021 gaccaacttc gacgacgtcc tggccggtgg cgagaagaag atcctcgaga ccgcagccac
     4081 cgtcgagaac ctgggcgcag ccgcgtacct gggccaggcc gcgaacatca agagcaagga
     4141 agtcctggcc gccgctctct cgatccacac ggtcgaggga cgtcacgccg ccgtgctgaa
     4201 cagcgtcatc ggcaagtcga tcgtgcccga cggcgcgttc gccaagccgg ccgacatggc
     4261 cacggtcctg aaggccgtca agccgttcat caaggcctag tccgagcgac ttcgagagga
     4321 gacgacagca atgtctgaca agaacatgaa ggcgcccgaa ctcgccaaca tcgaggtaca
     4381 gggcctgacc cgagagtcgt tcatcatgcg cggagccatc gcggccggat cggtctacgg
     4441 actgtccacc gtcggcccgt tcgtgagcca ggctctggcc cagggtgacg gggatgtgga
     4501 catcctcaac ttcgccctga cgctggagta cctggaggcg gcgttctaca ccgaggcgct
     4561 caagcaggtc ggtggactgc gcgccgaggt caaggagctc gccaccacca cgcgtgacga
     4621 cgagaacgag cacgtggacg ccatcgcggc cacggtcaag aagctgggcg gcaagcccgc
     4681 cgccaagccg ggggtcgact tcggcaaggc gttcgccagc gagaagacgt tcctgaccac
     4741 cgccgtggtg ttcgaggacc tgggtgtggg tgcctacaac ggcgccggcc ccagcatcaa
     4801 gtccaaggag gtgctcgcgg ctgccgggag catcgtgcag gtcgagggcc ggcacgccgg
     4861 cgcggtgcgc ctgctcaacg gcgacaagat cgcaccgtcg ccgttcgaca agggcatccc
     4921 gatggccgag gtgctgatga aggccaagcc cttcatcaag agctaggaag catcagctgc
     4981 cgcgctcagg gaccgccgtc gacggcggtc cctgtcgcgt cagcccgcct cctcgcgggg
     5041 cggcagctgg atctcctcct ccgcgccgga cacgccggcc ttgatctccc gcgagcgctc
     5101 cagctcggcg ttcagctccg cgcccagcag gacggcgatg ttggtgatcc acagccagac
     5161 caggaaggtc acggcgccgc cgagggtgcc gtacgtcttg ttgtacgagc cgaagttggc
     5221 cacgtacagg gcgaacagcg cggacgcgcc gacccacacg accagggcca gcaggctccc
     5281 gggtgtgacc cagcggatgc ccgtgaccct ggcgttcggc gaggcgtagt acaggagccc
     5341 gaggatcccc atgaccagga gcgccaggac cggccacttc gcgacgccgt agacggtcac
     5401 cgcctcctcg ctcaggccga gcgcgtcgcc gaccgcgccg gccagcgggc cgctgagcac
     5461 cagggccgtg atcaccaggc cgacggcgcc gacgatggtg atcgtcaccg ccagctggag
     5521 cggacgcagc ttgtagaagg gacggccctc ctgcacctcg tagatcacgt tcgaggcgcg
     5581 gatgaaggcg ccgatgtagc ccgaggccgc gttcagcgcg cccaggatgc ccagcaccag
     5641 caggacgccg gcggcgccgc ggtcctgggt caggccctcg atcgtgtcct gcag
//
LOCUS       DASUPW010000416         8229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1342191, whole genome shotgun sequence.
ACCESSION   DASUPW010000416 DASUPW010000000
VERSION     DASUPW010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342191"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VFY44_09340"
     CDS             complement(<1..265)
                     /locus_tag="VFY44_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910684.1"
                     /translation="MPTRLGDLTTVAARRGARLAHRARARAGRDGGRAAILLYHRVAT
                     PERDAWDLSVSPETFAAHMELLADRYRPLALADLLAAADAGTIP"
     gene            275..1243
                     /locus_tag="VFY44_09345"
     CDS             275..1243
                     /locus_tag="VFY44_09345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5910685.1"
                     /translation="MPDCGAVDAPEASVVIPTRNRREMLARTLGGALRQQEVELEVVV
                     VDDSTDDATKDYLDGLGDDRVRHVRGRARGVSDARNQGVAAAGGRWVAYLDDDDLWSP
                     VKLRRQIDLAEAAGAVFAWSGAIAVDPQLRPVPTTVPAAAVDGLTERLVVGNAVPAGC
                     SNVVARADAVARTEGYDERFAQVADWDMWLQLAAQGPAAATSEVHVAYVQHDENMLLA
                     GDTADPFEEFERLRQKHAALAAGLGTDFSRAHYARWVATRHRRAGDRALAARMLVRES
                     GDLRHAPGNLARAGAVLLGEPIVNRVRHRGATRAERPAWLDDYVAA"
     gene            complement(1228..2586)
                     /locus_tag="VFY44_09350"
     CDS             complement(1228..2586)
                     /locus_tag="VFY44_09350"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX5910686.1"
                     /translation="MSALRPSIGPARASSGLPTPYILAGAAACTLAGLAFAYVLTIFT
                     PPLPLVVAGSIGFAGLATLAVARLDSAVALGMLTLGVVVVEPAPPDAVFAVVIAVAAV
                     TGRFSLKGTPLSIFTGLALFLTLSIFSVIEAIEASKAVVYLAITVYLIVFSVWMAGYV
                     DSVKRSTMIVKAYVIGAALVGALSTLALFVPIPGGELLLTSDGLRAQGLFQDPNVYGP
                     FMVPAALLMLEELINPRLLTWRPTVKALVFVILVAAATFSYSRATWGSTVVGILVVLA
                     VLSLRRGGSRRAMWGLVLLATGFALATFVLSATGSLGFIEERATLQSYDTDRFAAQRL
                     GLELAFQKPLGIGPGQFEVISPVATHSTYVRALAEQGLLGLAAMLTILFGTLILALRN
                     AMLGRDAWGIGSATLLGAWIGLLVNSAVVDTAHWRILWFVAALIWAASMRHRWEGRQA
                     AT"
     gene            2631..4124
                     /locus_tag="VFY44_09355"
     CDS             2631..4124
                     /locus_tag="VFY44_09355"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX5910687.1"
                     /translation="MNTPPTTPDANEGEASGSVARNALVRMAGEILAKTASVVFFILI
                     ARKLGEEAFGDFTFALSLTSVLLFAAGLGTEELTTREIARDRSTVHLYITNVILIKTI
                     TSFLLVVASVLIVVLGSYPAEVQLTVLLVGAGVAFENTGRSYWSAFQAFERQEYVSLS
                     LIVQRFATLIGCIVVLVAGGGLVAVAAVFLGGAVLNLFTVRWALRRFVVKLRFDVDVA
                     RWWPIARAGLPIGVVTVLGGLTLRLDATLISFFTPGSGNAEVGYFGAAFRLVEATLFI
                     GWGFNAAALPWLAQQTGDALRHGFELGTKAVAGLLAPIGVILCVLAEPIIELVYGAQY
                     EPAVLPLRYLGLSVVAYSINFYFSTVLVARDKPTAFARAQLVALGVNVAVNAYVIPRH
                     GAAGAAAVALGTGALLAVLGAIQVQRVIGRASLLRGLLSPTVAALAMAGVIELTQLPL
                     VPAALLGLVVYGATALAVERVVYPDDLAMARVLVSRLRRRRVAALAD"
     gene            4129..4485
                     /locus_tag="VFY44_09360"
     CDS             4129..4485
                     /locus_tag="VFY44_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910688.1"
                     /translation="MSAADTKALIATAVERLLDAVPALRQLKLTIRLQLEAKGGDAPI
                     WRVELPGPKIDRDPAGDARLDVTIQRQHFNRLAEEGTLTEWARAYEKGHVKVAGDAGI
                     VKLLGSVIAKQLARQH"
     gene            complement(4493..5248)
                     /locus_tag="VFY44_09365"
     CDS             complement(4493..5248)
                     /locus_tag="VFY44_09365"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX5910689.1"
                     /translation="MPELPEVERARAALEQGALGRRIAAVDDADTWVCRPHAPGEIAG
                     ALTGATLTAAHRRGKAMWVETDRGVVLGLHLGMAGRIAVDEPPADRSWDRFSVQFEDG
                     GRLALHDRRRLGRVVLDPDLDRVGPDAAEVGRDAFRERVGRGSAPLKARIMDQGTIAG
                     VGNLLADETLWKAGLSPLLPAGDLDDAQLDHLRRVLRRATRSAIRKGGVHTGDLIPHR
                     NRGGTCPSCGAELRREAVGGRTTYWCPVEQPGP"
     gene            5308..5679
                     /locus_tag="VFY44_09370"
     CDS             5308..5679
                     /locus_tag="VFY44_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910690.1"
                     /translation="MSAEPTHAPVTYLEPVFSPPRPAQVTSDEALTELALALDTQRSA
                     NKAMVEAVKGMDRMLGDQRELNADLARRNEQLEAQMAEYAEAVTRQVADNNKLWCDLR
                     VAEHRLEEWLDKPAWRRLMRR"
     gene            complement(5734..5970)
                     /locus_tag="VFY44_09375"
     CDS             complement(5734..5970)
                     /locus_tag="VFY44_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX5910691.1"
                     /translation="MPGVFIDVELTDEARTDAELAQKLADACPVDIFAATEGGTEVVE
                     QNLDECILCGLCVDAAPAGAVVVKKLYDDTTLQR"
     gene            complement(5970..6881)
                     /locus_tag="VFY44_09380"
     CDS             complement(5970..6881)
                     /locus_tag="VFY44_09380"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX5910692.1"
                     /translation="MAVLLSENTSFIVQGITGREAVNLTRECLDYGSNIVGGVTPGRK
                     GREVHGVPVFDTVGQVIDHNGGQVPDGSVVTVPPAFTKDAVFEAIENGIKTVVIVTER
                     IPRGDVAQMVELADEHGARIIGPNCLGLIVPGVAKMGGIGGPAKNAEQAYQPGQVGVM
                     SRSGGMTTEISSTLTAAGLGVSTAISIGGDAIIGSTYAELMPHFEADEQTKAIVIYTE
                     PGGRMEAQLAEWVAENNSRLPIVAFMAGRFMDDDEMQGMSFGHAGTIVEGKEDSATEK
                     IARLEAAGIPVVERIDQIPDAVKEKLA"
     gene            complement(6881..8095)
                     /locus_tag="VFY44_09385"
     CDS             complement(6881..8095)
                     /locus_tag="VFY44_09385"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX5910693.1"
                     /translation="MRFFEYESRRLVEAAGIPVTKYGFATTDQEARQIAEDIGGPTVI
                     KSQVLSGGRMKAGGVKFADTPEEAAGHARDILKLEINGHMPRGVLVDPKAEVKQEYYA
                     GVVWDGTAKKPLILFSDMGGIDIEQVAEDHPDHVGRGHISNLKPVSDYQAKQVIAAAG
                     VTGKPLQRLTPIVARLAQLFRDRDMTLAEINPLAELTDGSFVALDAHMEMEEEAKPRQ
                     RKILAGIGIEEDDNREGYEPSPFEVAVSAIDAADHRGVIQGKDTGFHGNIGLVIGAGG
                     GSLTLTDAVRSQGGQPANYSEIGGNPSVAKAHGLATEVLKKDGVEKIAVMMSIVSNTR
                     VDIVARGVIKACLDLGKDPAETIAIFRIPGAWEEEGFKILDKYGIEYCDRSVSMHEAA
                     QRAVEKIQGAAA"
BASE COUNT         1109 a         2899 c         3036 g         1185 t
ORIGIN      
        1 cggggatcgt gccggcgtcg gcggcggcca ggaggtcggc cagcgccagc ggccggtagc
       61 ggtcggccag cagctccatg tgggcggcga aggtctcggg gctgacgctg aggtcccacg
      121 cgtcccgctc gggggtggcg acccggtggt agagcaggat cgctgcccgt ccgccgtcac
      181 ggcccgcacg ggcgcgggcc cggtgggcca ggcgcgcgcc tcggcgcgca gccacggtgg
      241 tcaggtcgcc gagccgggtg ggcatcccgg cagggtgcca gactgcggcg ccgtggacgc
      301 ccccgaggca tcggtcgtca ttccaacccg caaccgccgg gagatgctgg cgcgcaccct
      361 ggggggcgcg ctgcgccagc aggaggtcga gctggaggtc gtcgtggtgg acgacagcac
      421 cgacgatgcg accaaggact acctggacgg gctgggcgac gaccgcgtcc gtcacgtccg
      481 gggccgtgcc cgcggcgtgt cggacgcccg caaccagggt gtcgccgccg ccggcggccg
      541 ctgggtcgcg tacctggacg atgacgacct ctggtcaccg gtcaagctcc gccgccagat
      601 cgacctggcc gaggccgccg gcgccgtgtt cgcgtggtcc ggcgcgatcg cggtcgaccc
      661 ccagctgcgg cccgtgccca ccacggtgcc cgccgcggct gtggacggcc tgaccgagcg
      721 cctggtggtg ggcaacgccg tgcccgcggg ctgctccaac gtggtggcgc gcgcggacgc
      781 ggtggcccgg accgagggct acgacgagcg cttcgcccag gtggccgact gggacatgtg
      841 gctgcagctg gcggcccagg gcccggccgc cgcgaccagc gaggtgcacg tggcctacgt
      901 ccagcacgac gagaacatgc tgctggcggg ggacacggcc gacccgttcg aggagttcga
      961 gcgcctgcgc cagaagcacg ccgccctggc cgccgggctg ggcacggact tcagccgggc
     1021 gcactacgcg cgctgggtgg ccacgcgcca ccggcgcgcg ggcgaccggg cgctggccgc
     1081 gcggatgctg gtgcgcgaga gcggggacct gcgccacgcc cccggcaacc tggcgcgggc
     1141 cggcgcggtg ctgctggggg agccgatcgt gaaccgggtc cgccaccggg gcgccacgcg
     1201 ggcggagcgc ccggcgtggc tggacgacta cgtggcggcc tgacgccctt cccagcggtg
     1261 ccgcatcgac gccgcccaga tcagggccgc gacgaaccac aggatgcgcc agtgcgcggt
     1321 gtccaccacg gcgctgttca cgagcagccc gatccaggcg cccaggaggg tggccgagcc
     1381 gatcccccac gcgtcgcggc ccagcatcgc gttgcgcagg gccaggatga gcgtgccgaa
     1441 caggatggtg agcatggccg ccaggcccag cagcccctgc tcggccagcg cgcggacgta
     1501 ggtgctgtgg gtggcgacgg ggctgatcac ctcgaactgc cccgggccga tgcccagcgg
     1561 tttctggaac gccagctcca ggcccaggcg ctgggcggcg aagcggtcgg tgtcgtagct
     1621 ctggagcgtc gcgcgctcct cgatgaaccc cagcgagccg gtggccgaca gcacgaacgt
     1681 ggccagcgcg aagccggtgg ccagcagcac cagcccccac atggcgcgcc ggctcccgcc
     1741 ccgtcgcagc gagagtacgg ccagcaccac gaggatgccc accaccgtgc tgccccaggt
     1801 ggcgcgcgag tacgagaacg tggccgcggc cacgaggatc acgaacacca gggccttgac
     1861 ggtcgggcgc caggtcagca ggcgggggtt gatgagctcc tcgagcatca gcagcgcggc
     1921 cgggaccatg aacggcccgt acacgttcgg gtcctggaac aggccctgcg cccgcaggcc
     1981 gtcgctggtc agcagcagct cgccgcccgg gatcgggacg aacagcgcca gggtcgagag
     2041 cgcgccgacc agcgcggcgc cgatcacgta ggccttgacg atcatcgtgg agcgcttgac
     2101 cgagtccacg tagccggcca tccacacgct gaacacgatc aggtagaccg tgatggccag
     2161 gtagaccacg gccttcgagg cctcgatcgc ctcgatcacc gagaagatgc tcagcgtcag
     2221 gaacagcgcc aggccggtga agatgctgag cggcgtgccc ttgaggctga accgcccggt
     2281 cacggccgcc acagcgatca ccaccgcgaa caccgcgtcc gggggcgcgg gctcgaccac
     2341 cacgacgccc agcgtcagca tgcccagcgc gacggcggag tccagccggg ccacggccag
     2401 cgtggccagg cccgcgaacc cgatcgagcc cgccaccacc agcggcaggg gcggcgtgaa
     2461 tatcgtcagg acgtaggcga acgccagacc ggccagcgtg caggccgccg cgcccgcgag
     2521 tatgtacggg gtcggcagac cggaggacgc ccgcgccggg ccgatggatg ggcgaagagc
     2581 gctcacttcg ggctcagagc gtagtagagg cccgggtcgg taccccacgc gtgaacacac
     2641 ccccaacgac acccgacgcg aacgaaggcg aggcctcggg ctcggtcgcg cgcaacgccc
     2701 tggtcaggat ggccggcgag atcctggcca agacggcctc ggtcgtgttc ttcatcctga
     2761 tcgcccgcaa gctgggggag gaggccttcg gtgacttcac gttcgcgctg tcgctgacct
     2821 ccgtcctgct gttcgccgcc gggctaggca ccgaggagct gaccacccgc gagatcgccc
     2881 gggaccgctc cacggtccac ctgtacatca cgaacgtgat cctgatcaag acgatcacct
     2941 cgttcctgct ggtggtcgcg tccgtcctga tcgtggtcct gggctcgtac ccggccgagg
     3001 tgcagctgac ggtgctgctg gtgggcgcgg gggtggcgtt cgagaacacc ggccggtcgt
     3061 actggtcggc gttccaggcc ttcgagcggc aggagtacgt ctcgctgtcg ctgatcgtgc
     3121 agcggttcgc caccctgatc gggtgcatcg tggtgctggt ggcgggcggc ggcctggtgg
     3181 cggtcgcggc ggtgttcctg ggcggcgcgg tgctgaacct gttcacggtg cggtgggcgc
     3241 tgcggcggtt cgtggtcaag ctgcgcttcg acgtcgacgt ggcgcgctgg tggccgatcg
     3301 cccgggccgg cctgcccatc ggcgtggtca ccgtgctggg cggcctgacc ctgcgcctgg
     3361 acgcgaccct gatcagcttc ttcacgccgg ggtccgggaa cgccgaggtg ggctacttcg
     3421 gcgccgcgtt ccgcctggtc gaggccaccc tgttcatcgg ctggggcttc aacgcggccg
     3481 ccctgccgtg gctggcccag cagaccggtg acgcgctccg gcacggcttc gagctgggga
     3541 cgaaggccgt cgcgggcctg ctggcgccga tcggggtgat cctgtgcgtg ctggccgagc
     3601 cgatcatcga gctggtctac ggggcccagt acgagccggc ggtgctgccg ctgcgctacc
     3661 tgggcctcag cgtggtcgcg tactcgatca acttctactt ctccaccgtc ctggtggccc
     3721 gtgacaagcc gaccgcgttc gcgcgcgccc agctggtggc gctcggggtc aacgtggcgg
     3781 tcaacgccta cgtgatcccc cgccacggcg ccgccggcgc ggcggcggtc gcgctgggca
     3841 ccggggcgct cctggcggtg ctcggggcga tccaggtaca gcgcgtgatc gggcgggcgt
     3901 cgctgctacg ggggctgctc agcccgaccg tcgccgccct ggccatggcc ggcgtgatcg
     3961 agctgaccca gctgccgctg gtgcccgccg ccctgctcgg cctggtggtc tacggcgcga
     4021 ccgcgctggc cgtggagcgg gtggtctacc ccgacgacct ggccatggcc cgggtgctgg
     4081 tttcgaggct gcggcgccgc cgggtcgccg cactggcaga ctgagggcat gtccgccgcc
     4141 gacaccaagg ccctgatcgc caccgcggtc gagcggctgc tcgacgcggt cccggccctg
     4201 cgccagctca agctgaccat ccggctccag ctcgaggcca agggcggaga cgcgccgatc
     4261 tggcgcgtgg agctgcccgg cccgaagatc gaccgcgatc cggcgggcga cgcgcgcctg
     4321 gacgtgacca tccagcgcca gcacttcaac cgcctggccg aggagggcac gctgaccgag
     4381 tgggcccggg cctacgagaa gggccacgtc aaggtcgcgg gcgacgccgg catcgtgaag
     4441 ctgctgggca gcgtgatcgc caagcagctc gcgcgccagc actagcggcc cgtcaggggc
     4501 ccggctgttc gaccgggcac cagtaggtgg tgcgtccgcc gacggcctcg cggcgcagct
     4561 ccgcgccgca ggacgggcac gtgccgcccc ggttgcggtg ggggatcagg tcgcccgtgt
     4621 gcaccccgcc cttgcggatg gccgaccggg tggcgcggcg cagcacgcgc cgcaggtggt
     4681 ccagctgggc gtcgtccagg tcgcccgccg ggagcagcgg cgacaggccg gccttccaca
     4741 gcgtctcgtc ggccagcagg ttgcccacgc cggcgatggt gccctggtcc atgatccgtg
     4801 ccttcagcgg cgcggagccg cgtcccacgc gctctcggaa cgcgtcccgg cccacctcgg
     4861 ccgcgtcggg gcccacccgg tccaggtcgg ggtccagcac cacgcggccc agacggcgcc
     4921 ggtcgtgcag cgccagccgg ccgccgtcct cgaactgcac cgagaagcga tcccacgagc
     4981 ggtccgcggg cggctcgtcg acggcgatgc ggccggccat gcccaggtgc aggcccagca
     5041 cgacgccgcg gtcggtctcg acccacatcg ccttgccccg ccggtgggcc gccgtcaggg
     5101 tcgcgccggt cagcgcgccg gcgatctcgc cgggggcgtg ggggcggcag acccacgtgt
     5161 cggcgtcgtc cacggcggcg atccggcgcc ccagggcacc ctgctccagg gccgcgcggg
     5221 cccgctcgac ctcgggtagc tctggcactt ctgtctgtac gcgcgggcgg ggctaatggt
     5281 ttcgttcccg ctgccgagtg aacacccatg tcagccgaac cgacgcatgc ccccgtcacg
     5341 tacctcgagc cggtcttcag cccgcccagg ccggcccagg tcaccagcga cgaggcgctg
     5401 accgagctgg ccctggcgct ggacacgcag cgcagcgcca acaaggcgat ggtcgaggcg
     5461 gtcaagggga tggaccggat gctgggcgat cagcgtgagc tgaacgccga cctggcccgc
     5521 cgcaacgagc agctcgaggc ccagatggcc gagtacgccg aggccgtgac ccgccaggtg
     5581 gccgacaaca acaagctgtg gtgtgacctc cgcgtggccg agcaccggct cgaggagtgg
     5641 ctggacaagc cggcgtggcg ccggctcatg cgccgctagc gagaggagcg cagccgggcg
     5701 tcggccggcg agcaccgcat cgacacgttc gccctagcgc tgcagcgtcg tgtcgtcgta
     5761 gagcttcttg acgaccaccg cgccggcggg cgcggcgtcc acgcacaggc cgcagaggat
     5821 gcactcgtcc aggttctgct cgacgacctc ggtgccgcct tcggtggcgg cgaagatgtc
     5881 cacggggcag gcgtcggcca gcttctgggc cagctcggcg tcggtcctgg cctcgtccgt
     5941 cagttcgaca tcgatgaaca cgccaggcat caggccagct tctccttcac ggcgtcggga
     6001 atctggtcga tccgctccac cacggggatg ccggcggcct ccagccgcgc gatcttctcc
     6061 gtggccgagt cctccttgcc ctcgacgatg gttccggcgt ggccgaagct catgccctgc
     6121 atctcgtcgt cgtccatgaa gcggccggcc atgaaggcca cgatcggcag gcgcgagttg
     6181 ttctcggcca cccactcggc cagctgggcc tccatgcgcc cgccgggctc ggtgtagatg
     6241 acgatggcct tggtctgctc gtcggcctcg aagtggggca tcagctcggc gtaggtggag
     6301 ccgatgatgg cgtcgccgcc gatcgagatg gcggtggaca cgcccaggcc ggcggcggtc
     6361 agcgtggagg agatctcggt ggtcatgccg ccggagcggc tcatcacgcc gacctggccg
     6421 ggctggtagg cctgctcggc gttcttggcc gggccgccga tgccgcccat cttggccacg
     6481 ccgggaacga tcaggcccag gcagttgggg ccgatgatgc gggcgccgtg ctcgtcggcc
     6541 agctcgacca tctgggccac gtcgccgcgc gggatccgct cggtcacgat gaccacggtc
     6601 ttgatgccgt tctcgatcgc ctcgaacacc gcatccttgg tgaaggcggg ggggaccgtg
     6661 accacggagc cgtcgggcac ctggccgccg ttgtggtcga tgacctggcc gacggtgtcg
     6721 aacacgggca cgccgtgcac ctcacggccc ttgcggccgg gggtcacgcc gccgacgatg
     6781 ttggagccgt agtccaggca ctcgcgggtc aggttcacgg cctcgcggcc ggtgatgccc
     6841 tggacgatga aggacgtgtt ctcggagagg agaacggcca tcaggcagcc gctccctgga
     6901 tcttctcgac ggcgcgctgc gcggcctcgt gcatcgagac ggagcggtcg cagtactcga
     6961 tcccgtactt gtccaggatc ttgaagccct cttcctccca ggcgcccggg atgcggaaga
     7021 tggcgatggt ctcggcgggg tccttgccca ggtccaggca ggccttgatc acgccgcggg
     7081 cgacgatgtc cacgcgggtg ttggagacga tcgacatcat cacggcgatc ttctcgacgc
     7141 cgtccttctt caggacctcg gtggccaggc cgtgggcctt ggccacggac gggttgccgc
     7201 cgatctcgga gtagttggcg ggctggccgc cctgcgagcg cacggcgtcg gtcagcgtca
     7261 gcgagccgcc gcccgcgccg atgaccaggc cgatgttgcc gtggaagccg gtgtccttgc
     7321 cctggatcac gccgcggtgg tcggcggcgt cgatggccga gacggcgacc tcgaagggag
     7381 agggctcgta gccctcgcgg ttgtcgtcct cctcgatgcc gatgccggcc aggatcttgc
     7441 gctggcgcgg cttggcctcc tcctccatct ccatgtgcgc gtccagcgcc acgaaggagc
     7501 cgtccgtcag ctcggcgagg gggttgatct cggccagcgt catgtcgcgg tcgcggaaca
     7561 gctgggccag gcgggcgacg atgggggtca ggcgctggag cggcttgccg gtcacgccgg
     7621 cggccgcgat gacctgcttg gcctggtagt cggagaccgg cttgaggttg gagatgtggc
     7681 cgcgcccgac gtggtcgggg tggtcctcgg ccacctgctc gatgtcgatg ccgcccatgt
     7741 cgctgaacag gatcagcggc ttcttggcgg tgccgtccca gaccacgccg gcgtagtact
     7801 cctgcttgac ctcggccttg gggtcgacca ggacgccacg gggcatgtgg ccgttgatct
     7861 ccagcttgag gatgtcgcgg gcgtggccgg cggcctcctc gggcgtgtcg gcgaacttca
     7921 cgccgcccgc cttcatccgg ccgccggaga gcacctgcga cttgatcacc gtcggcccgc
     7981 cgatgtcctc ggcgatctgg cgggcctcct ggtcggtggt ggcgaacccg tacttggtga
     8041 ccgggatgcc cgctgcctcg accaggcggc ggctttcgta ctcgaagaag cgcatgagcg
     8101 gccgggaacc ctacaggtcc ggcggtttct gccgatccgt catacgatga ccctcttgcg
     8161 gcctgccgat gctcgaccct ctggcctcgc ggggcgcgaa cgagcgctcc tgggcgggat
     8221 cttggcgct
//
LOCUS       DASUPW010000417        14165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1342491, whole genome shotgun sequence.
ACCESSION   DASUPW010000417 DASUPW010000000
VERSION     DASUPW010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 14165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14165
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342491"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1457
                     /locus_tag="VFY44_09390"
     CDS             <1..1457
                     /locus_tag="VFY44_09390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5910694.1"
                     /translation="LGAILALALVLRLWGLTHKSLDGGDEPFSLALAQRPLGEMFELF
                     GFEANGTIYSILLWPLVRISEAEWMLRLPALVAGVLAVAAIWWAGRELVGPRAGLLAA
                     GLMAVNPYAIYQSQYARPFSLVMLFAAVSFAALALALSDGRRRWWVLYVAATALGAYG
                     NAVTAAILIPAHLVLVLVRNRDALKPWLLSVVGVGVALIPLAVALGIARGRRNPLYWL
                     DSPGPKDIVIAGQDFASGLPASRPLFALTALIAGVIVVLAARRGGKWELPAPVPVIAW
                     ALLPIVLLALVSQITPLFRSAYAIAAFPGLLLLIAACLDRLPRAALGGGLAALVVVAM
                     AGTVSQATSQHDETWRSATDWLKAEYRPGDRVLEDIPSINTVIGYYDDRFRAPNGELA
                     VLEWKDNPFPRTVVPYDDPDGYAGRTGPPTQAAIARLARGNRRLFVFLAEYSESLQGD
                     VPNSAALRWARRNCEVTEREETEITALLITGCPS"
     gene            1468..2271
                     /locus_tag="VFY44_09395"
     CDS             1468..2271
                     /locus_tag="VFY44_09395"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX5910695.1"
                     /translation="MHVIRRLRAEPHPARFIASRLLWRSGLCRRLTIGFPERGYRLRF
                     HPNAVSASLWVDRDKGADDRAVLGRSLAQGELAVDVGANVGALAVEAAALVGPQGTVY
                     AFEPNPHVFPYLEDNVALNDLKQVKCLNYAVGDTHEPVRFTADPRSDEFSRVDPEGAI
                     SVPSTTLDDAIPGDARIALLKVDVEGYEMAVFRGATRTLERTERIYFEVADEHFRRFG
                     YTTAELLALLRERGFEIYRGGRAGTDPVPLTEHSGDWDNLIAARPGISV"
     gene            complement(2261..3514)
                     /locus_tag="VFY44_09400"
     CDS             complement(2261..3514)
                     /locus_tag="VFY44_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910696.1"
                     /translation="MEAMQGDGRAGRAAGRARPRVGRGLRLGACLGLTLGGLGLGAPA
                     PAPALTDQREQGHARFFKGADGTLDRYIAGTRCRQAFGVYTRVRLFAPAGDRCVRRGF
                     RRALVYVNAQAIRVEDARANRRLDRLVLRDRRGRRLYVPFGCAKGTCPQYAADVASPA
                     WRRHVARQIRAVTRARYAGVFLDDVNFDRNVGDGRGRPVAPVRGGRTLSEAEWKAAMA
                     TLVRTARRAAPRRELMINTVWWKPESSLDDPVVAAGVAAATDFEIERGAEDTRRGQPF
                     DGLLAVIDRLHGLGLGVTLENYEATDRRRAEFELATHLLTTEGRDTFSAEWRSCPRAT
                     RRYKPCGRSFWRGYLTRLGPALGPREQRPDGLWQRRFRDGLVLVNPPGSPRREGALDR
                     MYQDLDGTPRVFVALDPDQALVLRR"
     gene            complement(3415..4479)
                     /locus_tag="VFY44_09405"
     CDS             complement(3415..4479)
                     /locus_tag="VFY44_09405"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX5910697.1"
                     /translation="MRNPRDFAKPLAVGAPTPTLEVPFTPSRMIHFFDPSNEKIVGKL
                     PDLVQKADVVLGNLEDAISVDNKEAAREGLVKVGREIDLGQAQLWTRINSLESPWALD
                     DVTRLVTEIGDKLDVIMVPKVEGAWDIHYMDRLLAQLEARAGLERPILIHALLETGIG
                     VVNLEEIATASPRMQGMSFGPADLAASRRMKTTRVGGGHPGYLVMNDPDPENPEAPRA
                     TAQQDLWHYSIARMVDACTSAGILPYYGPFGDIKDVAGCEAQFRAAFLLGCVGTWSLH
                     PVQIDIAKKVFSPDPDEVKFAQKVIEAIPDGRGVHMIDGKMQDDATWKQCKVMVELAE
                     QLAERDPELAEAYGLAPASA"
     gene            4598..4981
                     /locus_tag="VFY44_09410"
     CDS             4598..4981
                     /locus_tag="VFY44_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910698.1"
                     /translation="MSQNQPQSLDDIIERFKREISDHAAAERARVTAAADAAINRIHE
                     LEASLGERPGPAAEELRVPRRFERSVEPDPGKDIPAGAVLMARRMADEGHTRGEVAEF
                     LAENFRRIDVPAAVDHAFQDASAAS"
     gene            5037..5543
                     /locus_tag="VFY44_09415"
     CDS             5037..5543
                     /locus_tag="VFY44_09415"
                     /inference="COORDINATES: protein motif:HMM:NF039588.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX5910699.1"
                     /translation="MTDFTTDQREREASGGHPYGRYLEEFDEGAVYKHWPAKTVTEAD
                     DHAFCLLTMNHHPLHINDVYAAQSQQGRNVVVGPLVYSLALGMSVSDVSGKAIANLAT
                     DELSHPAPVFHGDTLFVESEVLEVKESRSKPDRGVVRVHTRVYNQDGVLVAEFKRSVL
                     IPRKTPGE"
     gene            complement(5568..5966)
                     /locus_tag="VFY44_09420"
     CDS             complement(5568..5966)
                     /locus_tag="VFY44_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX5910700.1"
                     /translation="MLLPASAAQANPAHAVVAKVNSYRAANGLPALRLSRSLSRSSYA
                     YARHLMRSDRFGHSSHIRASSRFNMLGENLAFSWGKRRSARIPVRGWARSAPHRAVLL
                     NRKFRYVGVGRVAGRFGSRRATIWVLHAGR"
     regulatory      complement(6012..6191)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            6267..7367
                     /locus_tag="VFY44_09425"
     CDS             6267..7367
                     /locus_tag="VFY44_09425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5910701.1"
                     /translation="MSAILMVTQPSDGGVFEHLVGLVAALRERGHRVAVAGPVEHEAE
                     RLGGDVHQLELVRSVSPRQDGPALARYARVVRAVRPDVIHAHSSKAGALARLGRVAHP
                     RTPVVYTPHGFAFNGYFESERERSAYRAVERALAPLASRVLCVCEAERRLAASVGPAG
                     RTRTVYNAVAEHVPGPPHPDLAAARGRGPVIGAVSQLRPGKGVETLVDAMPALLARHP
                     AVSVAVAGSGPLRSEIEAQARALGVQDSLLLLGQTDGPGPVLDGSDLFVNPSWAESFP
                     LSTLEAMSASVPVVVTDVGGAGEAVQDGRTGLLVPARDPGALADGLARLLDDPEAADG
                     LAGAALEEVRTRFTVDRMVDGTTAVYGELTAL"
     gene            complement(7364..8221)
                     /locus_tag="VFY44_09430"
     CDS             complement(7364..8221)
                     /locus_tag="VFY44_09430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX5910702.1"
                     /translation="MNPPTDRPRIAVLIPCYNDGELIGETLTSLLQDRDLEIVIIDDA
                     STDEATLETLRGLEAEGLTVVWRKENGGVARARADGLQASSAPYVYPLDADDVTIPGT
                     ISQMADRLDEDPGAAVCFGDYEEFGLTEVVRAVPEHLDPFRVAYANEYPVSSLFRRSV
                     LDDLGGWTAGGFDERSHEDWNLWMALVENGYRGVHLGPGQITYRRRLADGRLLGWGKQ
                     HHPMLYRKLKAAHPKLFADIDRYRRESDLPRSRKLLYPYIYGGRKRRAFEPKLKELLD
                     RVGFWTLRR"
     gene            complement(8265..9776)
                     /locus_tag="VFY44_09435"
     CDS             complement(8265..9776)
                     /locus_tag="VFY44_09435"
                     /inference="COORDINATES: protein motif:HMM:NF026235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEX5910703.1"
                     /translation="MKAAHRILLCSLLSALLALSFSAAASQAASPEAGSVKFTRAAES
                     DFDQFTKDPTAGEQESMRNRFWRMRTYAPYFDARLSWYKDAWTYKNLYAIYSDDAEAE
                     AHSDQILKDANGNRLYIPFDCDGQKCTQYAGDFGDPAFRARWIAAAKEQSANYRGIFI
                     DDVNMEVRVGNAKGEKVMPQDPRTGRTMTEADYRRYIAEFTEQIKAALPGKEIVHNAL
                     WFADHSDPSVRRELDAATHIELERGVNDDGLRGGNGQWSYERFMKRVDYLHSRGKGVI
                     FDSGADNEADVEYELASYFLVSGGNDGVGTPWRSNPRDWWQGYDVELGAPRGNRYEWN
                     GMLRRDFANGYVLVNQPDSPTRSARLPAGSRGPAGDPRGAVRLGAGQGKIVLGRVPAA
                     VGRPVLKVTTNPLTRLRARSSRTSRPVIRRARRTAKLRGRVKRATGGKVRVTVQRRVR
                     GGWRASRTVVTRVSKSGTFNRTFRSLPRGGYRIKATYGGQKSATSVRHFRIRR"
     regulatory      complement(9785..9864)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(9974..11590)
                     /locus_tag="VFY44_09440"
     CDS             complement(9974..11590)
                     /locus_tag="VFY44_09440"
                     /inference="COORDINATES: protein motif:HMM:NF026235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEX5910704.1"
                     /translation="MKTTRPAWLAAMLLLVMMAVPSLASATETPGPSAGKVNFAKAAQ
                     SDFDQYLLGTSTSRKQWMNQNYWRMRAYAPFFDTRTDWYGRGWAYENAYAIYRGWEGD
                     DARFILKDASGNRLYIPFACNGSHCTQYAADIGDPGWRASFINRAKARIARGYRGVFV
                     DDVNFAFNVSDGAGNLVAPRDPRTGRTMTHTDWQRYMAEFMEQLRAELPEQAEIVQNQ
                     VYFHVGLSSPYVRRAIDASTHIEVERGFNDTGITGGNGWFGYETVMAWVDYIHSRGKG
                     VVYDVQSGWGREYALGNYFLTSTGNDGLGMDDGGLPDNFWSGFATELGAPLNQRYKWN
                     GLFRRDFENGTVLVNQPGSPTRTVTPGGEWTDTAGNRRTSVTIGARDASVLRRASGEG
                     PVPTPEPDATPAPTFPTTPAPVTRPNTPKPTAAPAADPATGAVRAVRNTRRPAAAKKK
                     LTAIVKGRVRAATAGGRARSTRLSRVRVKLLRQVGGRWTVARTVTVRTDAQGRFTARA
                     TGLPAGRYKASASAPGRSVQLGWRGTLRLA"
     regulatory      complement(11599..11679)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            11833..13383
                     /locus_tag="VFY44_09445"
     CDS             11833..13383
                     /locus_tag="VFY44_09445"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEX5910705.1"
                     /translation="MSSLPPSNITSSSTGEDRIDGRRYLGALRRSRTLILFVVLIITG
                     AVVAASLVLPKQYQASTSIVLNVQPGIGDSDPTTIQRQLVTLQTLIISPDVLDEATKN
                     VPGQPDLGGKIDAKVNPDANIIDVTATDGDPALAAQMANTVASTFLKTRTDLERSQTA
                     RARERLQAEVARLQNTEGTEAQVNALQQRISQLSVNEESAGSDLQQVGRAEEPDAPSS
                     PQPLRNGLLAFVGSLFLGVLLALGRDQLSPRLGDPRELGRALDLRVLAGVPYVRGLRR
                     RRAAVMSGVEAEAYETVRAGVELVAGRDPAPRLLLVTGAVHGEGKTTAVWRLGNALAR
                     TGHKALILSADLRVPRMHEVAGIPLGLGLSDILAMIDWEGDRPDPEILDRSIVSVVSG
                     GPGKRRNGRLDMITSGTKAKDPGRLVTAGAMGAFLGYVRTLDYDYVLVDAPPLLGIVD
                     AQVLARYVDHVLLVNRLDRLSLDNVADLRQTLDRMEVAPLGIVVIGAKTEVSPYYTTR
                     RPPVGQPG"
     gene            13391..14119
                     /locus_tag="VFY44_09450"
     CDS             13391..14119
                     /locus_tag="VFY44_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX5910706.1"
                     /translation="MSARAVILAGGKGSRLAPYTAVIPKPLMPLGDRPILDVVLRQLA
                     SHGFGRVTIAVGYLAHLIQAVLGDGSEQGVEIDYHLETEPLGTAGPLATIADHESLLA
                     MNGDLLTDLDYGAFYAAHKASGNAMSVAVHRRTVTADYGVLDLEDADTDVVSVTGYHE
                     KPESQITVSMGVYAIEPEALALVPAGRFDIPDLVLKLLDEGMGVGAYPFEGYWLDIGR
                     HDDYDQAVKEFEEILPRLLPGGLS"
BASE COUNT         1838 a         5042 c         5207 g         2078 t
ORIGIN      
        1 tcctgggcgc gatccttgcg ctggcgctgg tcctgcgcct gtggggactg acccacaaga
       61 gcctggacgg cggcgacgag cccttctcgc tggcgctggc ccagcggccg ctgggcgaga
      121 tgttcgagct gttcggcttc gaggccaacg gcacgatcta ctcgatcctg ctgtggccgc
      181 tggtgcgcat cagcgaggcg gagtggatgc tgcgcctgcc cgcgctcgtg gccggcgtgc
      241 tggccgtggc ggccatctgg tgggcgggcc gggagctggt cggcccgcgc gccgggctgc
      301 tggccgccgg cctgatggcc gtgaacccgt acgccatcta ccagtcgcag tacgcgcgcc
      361 cgttctccct ggtgatgctg ttcgcggccg tctcgttcgc cgccctggcc ctggccctga
      421 gcgacggacg gcggcgctgg tgggtgctgt acgtggccgc caccgccctc ggggcctacg
      481 gcaacgcggt gaccgccgcc atcctgatcc cggcccacct ggtgctggtc ctggtgcgca
      541 atcgcgacgc gctgaagccg tggctgctct cggtggtggg ggtgggcgtg gccctgatcc
      601 ccctcgccgt ggcgctgggc atcgcccgcg ggcgccgcaa cccgctctac tggctggaca
      661 gccccgggcc caaggacatc gtgatcgcgg gccaggactt cgccagcggg ctgccggcct
      721 cgcggccgct gttcgcgctc accgccctga tcgcgggcgt gatcgtggtg ctggccgcgc
      781 gaaggggcgg caagtgggag ctgccggccc cggtgcccgt gatcgcctgg gcgctgctgc
      841 cgatcgtcct gctggccctg gtgtcgcaga tcaccccgct gttccgctcg gcgtacgcga
      901 tcgccgcgtt ccccgggctg ctgctgctga tcgccgcgtg cctggaccgg ctgccgcggg
      961 cggcgctggg gggcgggctg gccgcgctgg tcgtggtggc catggccgga accgtcagcc
     1021 aggccacctc ccagcacgac gagacctggc gcagcgcgac cgactggctg aaggccgagt
     1081 accggcccgg cgaccgcgtg ctggaggaca tccccagcat caacaccgtc atcggctact
     1141 acgacgaccg cttccgcgcc cccaacggcg agctcgcggt gctggagtgg aaggacaacc
     1201 cgttcccgcg caccgtggtg ccctacgacg accccgacgg ctacgccggc aggaccggcc
     1261 cgcccacgca ggcggccatc gcccgcctgg cgcggggcaa ccggcgcctg ttcgtgttcc
     1321 tggccgagta ctccgagtcg ctccagggcg acgtgcccaa cagcgccgcc ctgcgctggg
     1381 cgcgccgcaa ctgcgaggtc accgagcgcg aggagaccga gatcaccgcc ctgctgatca
     1441 ccggctgccc gtcgtagact cgccgccgtg cacgtgatcc gacgactgcg ggccgagccg
     1501 catccggccc gcttcatcgc ctcgcggctg ctgtggcgct cgggcctgtg ccggcgcctg
     1561 acgatcgggt tccccgagcg cggctaccgg ctgcgcttcc atcccaacgc cgtctcggcc
     1621 tccctgtggg tggaccgcga caagggcgcc gacgaccgcg cggtcctggg ccgcagcctg
     1681 gcccaggggg agctggcggt cgacgtgggg gccaacgtgg gcgcgctggc cgtggaggcc
     1741 gcggcgctgg tcggcccgca gggcaccgtc tacgccttcg agcccaaccc gcacgtgttc
     1801 ccctacctcg aggacaacgt ggcgctgaac gacctgaagc aggtcaagtg cctgaactac
     1861 gcggtcgggg acacccacga gccggtgcgc ttcaccgccg acccgcgcag cgacgagttc
     1921 agccgcgtgg acccggaggg cgcgatctcg gtgcccagca ccaccctgga cgacgcgatc
     1981 cccggcgacg cgcggatcgc gctgctgaag gtcgacgtgg agggatacga gatggccgtc
     2041 ttccgcggcg ccacccggac cctggagcgc accgagagga tctacttcga ggtggccgac
     2101 gagcacttcc ggcgcttcgg ctacaccacg gccgagctgc tggcgctgct gcgcgagcgc
     2161 gggttcgaga tctaccgcgg cggtcgcgcc ggcaccgatc cggtgccgct gaccgagcac
     2221 tccggcgact gggacaacct gatcgccgcc cgtcccggga tcagcgtctg agcaccagcg
     2281 cctgatcggg gtccagcgcc acgaacaccc gcggcgtccc gtccaggtcc tggtacatgc
     2341 ggtccagcgc accttcgcgg cgcgggctcc cgggcgggtt gaccagcacc aggccgtcgc
     2401 ggaagcgccg ctgccacagg ccgtcgggcc gctgctcgcg cgggcccagc gccggtccca
     2461 ggcgcgtcag gtatccgcgc cagaacgagc ggccacacgg cttgtagcgg cgggtcgcgc
     2521 gcgggcacga gcgccactcg gccgagaagg tgtcccggcc ctcggtggtc agcaggtggg
     2581 tggccagctc gaactcggcc cggcggcggt cggtggcctc gtagttctcc agcgtcacgc
     2641 ccagccccag cccgtgcagc cggtcgatca cggccagcag cccgtcgaag ggctggccgc
     2701 gccgggtgtc ctcggcgccg cgctcgatct cgaagtccgt ggcggcggcc acgccggcgg
     2761 ccaccaccgg gtcgtccagc gacgactcgg gcttccacca cacggtgttg atcatcagct
     2821 cgcgcctcgg cgcggcgcgg cgcgctgtgc gcaccagcgt ggccatggcc gccttccact
     2881 cggcctcgga cagcgtccgg ccgcccctga cgggggccac cggccggccg cggccgtcgc
     2941 ccacgttgcg gtcgaagttc acgtcgtcca ggaacactcc ggcgtagcgc gcccgcgtca
     3001 cggcacggat ctggcgcgcc acgtgccgcc gccaggcggg ggaggccacg tccgcggcgt
     3061 actgcgggca ggtgcccttg gcgcagccga acggcacgta caggcgccgg ccgcggcggt
     3121 cgcgcagcac cagccggtcc aggcgccggt tcgcgcgcgc gtcctcgacc cggatggcct
     3181 gggcgttcac gtacaccagg gccctccgga acccgcgccg cacgcaccgg tcgccggcgg
     3241 gcgcgaacag gcggacgcgg gtgtagacgc cgaaggcctg ccggcaccgg gtgccggcga
     3301 tgtagcggtc gagcgtcccg tcggcgccct tgaagaagcg ggcgtggccc tgctcgcgct
     3361 gatcggtcag ggcgggggcc ggcgcgggcg cgcccagccc cagcccgccc agcgtcaggc
     3421 cgaggcaggc gccaagccgt aggcctcggc caactcgggg tcgcgctcgg ccagctgctc
     3481 tgccagctcg accatcacct tgcattgctt ccatgtggcg tcgtcctgca tcttcccgtc
     3541 gatcatgtgc accccgcgtc cgtccgggat ggcctcgatc accttctggg cgaacttcac
     3601 ctcgtcggga tcggggctga acaccttctt agcgatgtcg atctgcacgg ggtgcagtga
     3661 ccaggtgccc acgcagccca gcaggaacgc cgcgcggaac tgcgcctcgc acccggccac
     3721 gtccttgatg tccccgaacg ggccgtagta gggcaggatc ccggccgacg tgcaggcgtc
     3781 gaccatgcgc gcgatcgagt agtgccacag gtcttgctgc gcggtcgcgc gcggggcctc
     3841 ggggttctcg gggtcggggt cgttcatgac caggtagccg gggtggccgc cgccgacgcg
     3901 tgtggtcttc atccgccgtg aggcggccag gtcggccggg ccgaagctca tgccctgcat
     3961 ccgcggcgag gccgtggcga tctcctcaag gttcaccacg ccgatgcccg tctccagcag
     4021 cgcgtggatc aggatcggcc gctccaggcc ggcgcgcgcc tccagctggg ccagcagccg
     4081 gtccatgtag tggatgtccc aggcgccctc gaccttgggg accatgatca cgtcgagctt
     4141 gtcgccgatc tcggtgacca gccgggtcac gtcgtccagc gcccacgggg actccagcga
     4201 gttgatccgg gtccacagct gcgcctggcc caggtcgatc tccctgccga ccttgacgag
     4261 cccctcgcgc gccgcctcct tgttgtccac cgagatggcg tcctcgaggt tgcccaggac
     4321 cacgtcggcc ttctgcacca ggtcgggcag cttgcccacg atcttctcgt tcgaggggtc
     4381 gaagaagtgg atcatccgcg agggcgtgaa cggcacctcg agggtcggcg tgggggcgcc
     4441 cacggccagt ggcttggcga agtctcgggg gttgcgcatg gccgtgaatc taccgttacc
     4501 gaagttcgcg gagatccccg ggcgccgcta ctttggggtc agtacttggt tcggagacgg
     4561 gaggcctccg catcgcccat ccacggaagg acggtcaatg tcgcaaaacc agccgcagag
     4621 cctcgatgac atcatcgagc gcttcaagcg tgagatctcg gaccacgctg ccgccgagcg
     4681 cgcgcgggtg acggccgccg ccgacgctgc catcaaccgc atccacgagc tcgaggcgtc
     4741 gctgggggag cgaccgggcc ccgccgccga ggagctgcgt gtcccgcgcc gcttcgagcg
     4801 cagcgtcgag cccgatccgg gcaaggacat cccggccggc gccgtgctca tggcccgccg
     4861 gatggccgac gagggccaca cacgcggcga ggtcgccgag ttcctggccg agaacttccg
     4921 ccgcatcgac gtgcccgccg cggtcgacca cgccttccag gacgcctccg cggcctcctg
     4981 agccacgggc gggtccggcc gccccggcag gcgagtcact aacctgccgc ggcgatatga
     5041 ccgacttcac cacagaccag cgcgagcgcg aggcctccgg cggccacccc tacggccgct
     5101 atctcgagga attcgacgag ggcgccgtgt acaagcactg gcccgccaag accgtcaccg
     5161 aggcggacga ccacgcgttc tgcctgctga ccatgaacca ccacccgctg cacatcaacg
     5221 atgtgtacgc ggcccagtcc cagcaggggc gcaacgtggt cgtgggcccg ctggtctact
     5281 cgctggccct gggcatgtcg gtcagcgacg tgtccggcaa ggcgatcgcc aacctggcca
     5341 ccgacgagct ctcccacccg gcgcccgtct tccacggcga cacgctgttc gtggagagcg
     5401 aggtgctcga ggtcaaggag tcccgctcca agcccgaccg cggcgtggtc cgcgtgcaca
     5461 cccgggtcta caaccaggac ggcgtcctgg tggccgagtt caagcgctcg gtgctgatcc
     5521 ccaggaagac gccgggggag taacccccgg cccccgccgg tcgctgatca gcgaccggcg
     5581 tgcaggaccc agatggtggc ccggcgtgag ccgaaccgcc cggccacgcg gccgacgccg
     5641 acgtagcgga acttgcggtt cagcagcacc gcgcggtgcg gcgccgagcg ggcccagccg
     5701 cggacgggga tgcgggcgct gcggcgcttg ccccagctga aggcgaggtt ctcgcccagc
     5761 atgttgaagc gggagctggc gcggatgtgg gacgagtggc cgaagcggtc cgagcgcatc
     5821 aggtgccgcg cgtaggcgta ggagctgcgc gacaggctgc gcgacagccg cagcgcgggc
     5881 aggccgttgg cggcgcggta gctgttgacc ttcgcgacca cggcgtgggc ggggttggcc
     5941 tgggcggcgg aggccggcag caacatggcg gcgcaggcca gtacaacgag aacgctggtt
     6001 ctcctcacgg gcatctctct ttcgtcggcc tgcggggtta gctgacgggc gagcgcagaa
     6061 agagcgacgc gcttcaccgg gttgagcact caatccggcg attagcccct gacggccacg
     6121 gtgaccgtcc atcttggttc ccccgctcgt cgcgccctga cggactgccg acgattaggc
     6181 ggttgttggc gcagaaggta ccaaaggcgc tggctggcgt cctggctagc atggcgcggc
     6241 cggatgcgac cccgtacaca gcgcgcatga gcgccatcct gatggtcacc cagccctccg
     6301 acggcggggt cttcgagcac ctggtggggc tggtcgccgc gctgcgggag cgcggccacc
     6361 gggtggcggt ggcggggccc gtcgagcacg aggccgagcg cctgggcggc gacgtccacc
     6421 agctggagct ggtgcggtcg gtgtcgcccc gccaggacgg cccggcgctg gcccgctacg
     6481 cccgggtcgt gcgcgcggtc cggccggacg tgatccacgc gcacagctcc aaggcgggcg
     6541 cgctggcacg gctggggcgc gtggcccatc cgcgcacgcc ggtggtgtac acgccgcacg
     6601 gcttcgcctt caacgggtac ttcgagagcg agcgcgaacg cagcgcctac cgcgcggtcg
     6661 agcgtgctct ggccccgctg gccagccggg tcctgtgcgt gtgcgaggcc gagcggcggc
     6721 tggccgcctc ggtcggcccc gcgggccgca cccggacggt ctacaacgcg gtcgccgagc
     6781 acgtgcccgg gccgccgcac ccggacctgg ccgcggcccg cgggcgcggc ccggtgatcg
     6841 gggcggtgtc gcagctgcgg cccggcaagg gggtcgagac gctggtggac gccatgccgg
     6901 cgctgctggc gcgccacccg gcggtgtccg tcgcggtggc cgggtccggt ccgctgcgct
     6961 cggagatcga ggcccaggcg cgggcgctgg gggtccagga ctcgctgctg ctgctgggcc
     7021 agaccgatgg cccggggccc gtgctggacg gcagcgacct gttcgtgaac ccctcgtggg
     7081 ccgagtcgtt ccccctgtcc acgctggagg ccatgtcggc ctcggtgccg gtcgtggtca
     7141 ccgacgtcgg cggcgcgggc gaggccgtcc aggacggccg gaccggcctg ctggtgcccg
     7201 cccgtgaccc cggtgcgctg gccgacgggc tggcccggct gctggacgat cccgaggccg
     7261 cggacgggct ggccggcgcc gcgctcgagg aggtgcgcac gcgcttcacg gtggaccgga
     7321 tggtcgacgg caccaccgcg gtgtacggcg agctgacggc gctctagcgc cgcagggtcc
     7381 agaagccgac gcggtccagc agctccttga gcttgggctc gaacgcccgt cgcttgcgcc
     7441 cgccgtagat gtacggatac aggagcttgc gcgagcgcgg caggtccgac tcgcgccggt
     7501 agcggtcgat gtccgcgaac agcttggggt gggcggcctt gagcttgcgg tagagcatcg
     7561 ggtggtgctg cttgccccag cccaggaggc gcccgtccgc cagccggcgc cggtaggtga
     7621 tctggcccgg cccgaggtgc acgccccggt agccgttctc gaccagcgcc atccacaggt
     7681 tccagtcctc gtggctgcgc tcgtcgaagc cgccggccgt ccagccgccc aggtcgtcga
     7741 gcaccgagcg ccggaacagc gacgacacgg ggtactcgtt ggcgtacgcg acgcggaacg
     7801 ggtccaggtg ctcgggcacg gcccggacca cctcggtcag gccgaactcc tcgtagtcgc
     7861 cgaagcagac cgccgcgccg gggtcctcgt ccaggcgatc ggccatctgg ctgatcgtgc
     7921 ccgggatggt cacgtcgtcc gcgtcgaggg ggtagacgta gggcgccgag ctggcctgca
     7981 ggccgtccgc gcgggcgcgc gcgaccccgc cgttctcctt gcgccagacc acggtcaggc
     8041 cctcggcctc gaggccgcgc agggtctcca gcgtggcctc gtcggtggag gcgtcgtcga
     8101 tgatcacgat ctccaggtca cggtcctgga gcagcgacgt gagcgtctca ccgatcagct
     8161 ccccatcgtt gtaacagggg atcagaacgg cgatccgggg gcggtcggtc ggcggattca
     8221 ccggcgcgag gttacccggc gcccgtaggc ggatcgtccg ggggtcagcg ccggatgcgg
     8281 aagtggcgca ccgacgttgc gctcttctgg ccgccgtagg tggccttgat gcggtagccg
     8341 ccgcggggga gggagcggaa ggtgcggttg aaggtgcccg acttcgacac ccgcgtgacc
     8401 acggtgcgcg aggcgcgcca gccgccgcgg acgcggcgct gcacggtcac gcggaccttg
     8461 ccgccggtcg cgcgcttgac gcggccgcgg agcttcgcgg tccgccgggc gcgccggatg
     8521 accgggcgcg aggtgcgcga gctgcgggcg cggaggcggg tcagggggtt ggtggtcacc
     8581 ttcagcaccg ggcggccgac cgcggcgggc acgcggccca gcacgatctt gccctggccg
     8641 gcgcccaggc ggacggcgcc gcgggggtcg ccggcggggc cgcggctgcc ggccggcagc
     8701 ctggccgagc gggtggggga gtcgggctgg ttgaccagca cgtagccgtt ggcgaagtcg
     8761 cggcgcagca tgccgttcca ctcgtagcgg ttgccgcgcg gggcgcccag ctcgacgtcg
     8821 tagccctgcc accagtcgcg cgggttcgag cgccaggggg tgccgacgcc gtcgttgccg
     8881 ccggagacca ggaagtagga ggccagctcg tactccacgt cggcctcgtt gtcggcgccg
     8941 gagtcgaaga tgacgccctt gccgcggctg tgcaggtagt cgacgcgctt catgaagcgc
     9001 tcgtagctcc actggccgtt gccgccgcgg aggccgtcgt cgttgacgcc ccgctccagc
     9061 tcgatgtgcg tggcggcgtc cagctcgcgg cggaccgagg gatcgctgtg atcggcgaac
     9121 cagagggcgt tgtgcacgat ctccttgccg ggcagcgcgg ccttgatctg ctcggtgaac
     9181 tcggcgatgt agcggcggta gtcggcctcg gtcatcgtgc ggccggtgcg cgggtcctgg
     9241 ggcatgacct tctcgccctt ggcgttgccc acgcggacct ccatgttcac gtcgtcgatg
     9301 aagatgccgc ggtagttggc ggactgctcc ttggcggcgg cgatccagcg ggctcggaag
     9361 gccgggtcgc cgaagtcgcc ggcgtactgg gtgcacttct ggccgtcgca gtcgaagggg
     9421 atgtacaggc ggttgccgtt cgcgtccttg aggatctggt cggagtgggc ctcggcctcg
     9481 gcgtcgtcgc tgtagatggc gtagaggttc ttgtaggtcc aggcgtcctt gtaccaggac
     9541 aggcgcgcat cgaagtaggg ggcgtaggtg cgcatccgcc agaagcggtt gcgcatcgac
     9601 tcctgctcac cggcggtggg gtccttggtg aactggtcga agtcggactc ggcggcgcgg
     9661 gtgaacttga ccgagccggc ctcgggggag gcggcctggg aggcggcggc cgagaagctc
     9721 agagccagga gggcggacag cagtgagcac agcaggatgc gatgggcggc cttcacggtt
     9781 tcctttcggc ggctgggctg cctctggagg gagaggcccc ttgctttgcg tccccgcctc
     9841 gcggcgggtt tgcctttgtc ggggagaccg ctgggtctcc gcgcttactg tccctatcgg
     9901 cccccgcccg gctgcctgaa ggggccgtcg accgtcactg gacgcatgtg cgacctccgc
     9961 tgtgggcctg tgctcaggcc agccgcagcg tgccgcgcca gcccagctgg acgctgcgcc
    10021 ccggagcgct ggcgctggcc ttgtagcgcc cggcgggaag gccggtggcg cgggcggtga
    10081 agcggccctg cgcgtcggtc ctaaccgtga ccgtgcgggc cacggtccag cggccgccga
    10141 cctggcgcag cagcttcacc cggacccgcg acaggcgcgt cgagcgggcg cggccgccgg
    10201 ccgtggcggc gcggacgcgg cccttgacga tcgcggtcag cttcttcttg gcggcggccg
    10261 gcctgcgggt gttgcgtacg gcgcgaaccg cgccggtggc cgggtcggcc gccggtgccg
    10321 cggtcggctt cggggtgttc ggtcgggtga ccggtgcggg cgtcgtgggg aacgtggggg
    10381 cgggggtggc gtcgggctcg ggcgtgggca ccgggccctc gccggaggcg cgcctgagga
    10441 ccgacgcgtc gcgggcgccg atggtgaccg aggtgcggcg gttgccggcc gtgtcggtcc
    10501 actcgccgcc gggcgtgacc gtccgggtgg gcgagccggg ctggttgacc agcacggtgc
    10561 cgttctcgaa gtcacggcgg aagaggccgt tccacttgta gcgctggttc agcggcgcgc
    10621 ccagctcggt ggcgaacccg ctccagaagt tgtccggcag gccgccgtcg tccatgccca
    10681 ggccgtcgtt gccggtggag gtcaggaagt agttgcccag ggcgtactcg cggccccagc
    10741 ccgactggac gtcgtacacc acgcccttgc cgcgggagtg gatgtagtcg acccacgcca
    10801 tcacggtctc gtatccgaac cagccgttgc cgccggtgat gccggtgtcg ttgaagccgc
    10861 gctcgacctc gatgtgggtc gaggcgtcga tcgcgcgcct gacgtagggg ctggacaggc
    10921 cgacgtggaa gtagacctgg ttctggacga tctcggcctg ctcggggagc tcggcccgga
    10981 gctgctccat gaattccgcc atgtagcgct gccagtcggt gtgggtcatc gtgcggccgg
    11041 tgcgcgggtc gcgcggcgcg accaggttgc cggcgccgtc gctgacgttg aaggcgaagt
    11101 tcacgtcgtc cacgaacacg ccccggtagc cgcgggcgat gcgggccttg gcgcggttga
    11161 tgaagctcgc gcgccagccc gggtcgccga tgtcggcggc gtactgggtg cagtgcgagc
    11221 cgttgcaggc gaacgggatg tacaggcggt tgccgctcgc gtccttgagg atgaagcggg
    11281 cgtcgtcgcc ctcccagccg cggtagatcg cgtaggcgtt ctcgtacgcc cagccgcgcc
    11341 cgtaccagtc ggtccgcgtg tcgaagaagg gcgcgtaggc gcgcatccgc cagtagttct
    11401 ggttcatcca ctgcttgcgg ctcgtcgagg tgcccagcag gtactggtcg aagtcggact
    11461 gcgcggcctt ggcgaagttg accttgccgg cggagggtcc gggggtctcg gtcgcggagg
    11521 ccaggctcgg gacggccatc atcactagga ggagcatggc ggcgagccac gcggggcggg
    11581 tggtcttcac ggtgtccttt cggcggctgg gctgcctccc gggcggggag accccttgct
    11641 ttgcgtcccc gtctcgcgac gggtttgcct ttgtcgagga gggctgtggt cagccctgcc
    11701 gaacttcacc cactgaatcg gccgtgcggg ggttctccgt aacccgcggg tgccgctttg
    11761 gacgcatgtg tgagcatgct cgtccggtcg tggcgcatac gatgtcccga tgccccggca
    11821 ggggtagcca ccatgagcag tctgcccccg agcaacataa cctcctccag caccggcgaa
    11881 gaccgcatcg acggtcgccg ttacctgggc gcgctccgcc gcagccggac gctcatcctc
    11941 ttcgtcgtcc tgatcatcac cggcgccgtg gtcgcggctt cgctggtgct gcccaagcag
    12001 taccaggcct cgacctcgat cgtgctcaac gtccagccgg gcatcgggga ctccgacccc
    12061 accacgatcc agcgccagct ggtcacgctg cagacgctga tcatctcgcc cgacgtgctg
    12121 gacgaggcca ccaagaacgt ccccggccag cccgacctcg ggggcaagat cgacgccaag
    12181 gtcaacccgg acgcgaacat catcgacgtc accgccaccg acggcgaccc cgcgctggcg
    12241 gcccagatgg ccaacacggt ggccagcacc ttcctcaaga cgcgcaccga cctcgagcgc
    12301 agccagaccg cgcgtgcgcg cgagcgcctc caggccgagg tcgcccggct ccagaacacc
    12361 gaggggaccg aggcccaggt caacgcgctg cagcagcgca tcagccagct cagcgtcaac
    12421 gaggagagcg ccggctcgga cctccagcag gtcggccgcg ccgaggagcc cgacgcgccc
    12481 agttctcccc agcccctgcg caacggcctg ctggcgttcg tcggctcgct cttcctcggc
    12541 gtcctgctcg ccctcggccg cgaccagctg tcgccgcgcc tgggcgaccc gcgcgagctc
    12601 ggccgcgcgc tcgacctccg cgtcctcgcg ggcgtgccct acgtgcgcgg cctgcgccgc
    12661 cgccgtgctg ccgtcatgag cggggtcgag gccgaggcct acgagaccgt ccgcgccggc
    12721 gtggagctcg tcgccgggcg cgacccggcg ccacgcctgc tcctggtcac gggcgcggtg
    12781 cacggcgagg gcaagaccac cgccgtgtgg cggctgggca acgcgctcgc ccgcaccggg
    12841 cacaaggccc tgatcctctc cgccgacctg cgcgtgccgc gcatgcacga ggtcgccggg
    12901 atcccgctcg ggctcggcct ctcggacatc ctggccatga tcgactggga gggcgaccgg
    12961 cccgaccccg agatcctcga ccgctcgatc gtctccgtcg tgtccggcgg acccggcaag
    13021 cgccgcaacg gccggctgga catgatcacc agcggcacca aggccaagga cccgggccgg
    13081 ctcgtgaccg ccggcgccat gggggcgttc ctcggctacg tgcgcacgct ggactacgac
    13141 tacgtgctgg tcgacgcccc gccgctgctg ggcatcgtcg acgcccaggt gctggcccgg
    13201 tacgtcgacc acgtgctgct ggtcaaccgc ctcgaccgcc tgagcctgga caacgtcgcc
    13261 gacctgcgcc agacgctgga ccggatggag gtcgccccgc tgggaatcgt ggtgatcggc
    13321 gccaagaccg aggtctcgcc gtactacacc acccggcgcc cgcccgttgg ccaacccggc
    13381 tgagccgccc gtgagcgccc gggccgtgat cctggcgggg ggcaagggct cgcgcctggc
    13441 gccctacacc gcggtgatcc ccaagccgtt gatgccgctg ggcgaccgcc cgatcctgga
    13501 cgtcgtcctg cgccagctgg ccagccacgg gttcggccgc gtgaccatcg cggtcggcta
    13561 cctggcccac ctgatccagg ccgtcctggg cgacggcagc gagcagggcg tcgagatcga
    13621 ctaccacctg gagaccgagc cgctgggcac cgcgggcccg ctggccacga tcgccgacca
    13681 cgagagcctg ctggccatga acggcgacct gctcaccgac ctcgactacg gcgccttcta
    13741 cgccgcccac aaggcgtcgg gcaacgccat gtcggtcgcg gtgcaccggc gcacggtcac
    13801 cgccgactac ggcgtgctgg acctggagga cgccgacacc gacgtggtgt cggtcaccgg
    13861 gtaccacgag aagcccgaga gccagatcac ggtctcgatg ggcgtctatg cgatcgagcc
    13921 ggaggcgctg gccctggtgc ccgccggccg gttcgacatc cccgacctgg tcctgaagct
    13981 gctggacgag ggcatggggg tgggggcgta cccgttcgag ggctactggc tggacatcgg
    14041 ccgccatgac gactacgacc aggcggtcaa ggagttcgag gagatcctgc cccggttgct
    14101 gccgggcggc ttgagctagc tgctccttgt tgggcgcgtc gggcgtttgc ccggccgcgc
    14161 cgccg
//
LOCUS       DASUPW010000418         4421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_788998, whole genome shotgun sequence.
ACCESSION   DASUPW010000418 DASUPW010000000
VERSION     DASUPW010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788998"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VFY44_09455"
     CDS             <1..745
                     /locus_tag="VFY44_09455"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5910707.1"
                     /translation="VEWVSNIGLIARASLRLAQDRPAEAVADLRAHREIVQRFGWREF
                     ARSFPNAMLATALAAAGHKDEARELARQEAAFARDTGRHSCLVASLTALGRAQEGKEA
                     VATLAEAVRASAHSQSPVIRARALVELGAALRRDGQRVDARERLREGGELAGTAGARL
                     LQEQALEELSIAGARQRDPFSSGVDALTPSERRVAEHAARGMTNREIAETLFVTQKTI
                     EVHLGNTYGKLGIRSRNQLQAALEGPVSV"
     gene            760..3219
                     /locus_tag="VFY44_09460"
     CDS             760..3219
                     /locus_tag="VFY44_09460"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX5910708.1"
                     /translation="MAGARRHPWLTCALALGILCAGSTGAQAKILQAESVLPPGQSGY
                     VSVPGVVSGTGSPHLDDQRTLFTSFGFKSALFGQGGQTELPKAGVKVVRDRYGVPAVT
                     GASDYDAWWGVGYAVAQDRLFQLELFRRATSGRLAEILGDGYLDDDLIARRDYYTDAE
                     LDAMLGRIPAQLRQRAEAYRDGINAWSSKVRSDPSLLPGEFAALGATPPTWTLRDTAR
                     VGVFLARTVPSSDGREIDNARALRELGPKGFDKLLPLRTKGALPTVPASSGRFPSQPG
                     RSVRQEKAAFKRSQGALKGVSLPEPAASASASGTPRSSVIPHGGSYMWAIGRPAKKVR
                     KCRTVKRKGRKAKRRCRTTYRRPKKPGNAYLFNGPQLGYSVPELFVEYELHSPTQDIR
                     GVSAAGIPVLGIGHNGHLAWGFTSGLSDEDDLYAEKLTGTEAYRYKGASRPMDCRTER
                     FDFKAPVTDLPDLVGTPGRLSGSRTERICRTLHGPVQERAGGTAYARRYAIWGRELET
                     LVGLSALNDAKTVRQADTALQGVTWNENVMAADERGDIGFWHPGLHPLRPKGYDERLP
                     YPGTGEAEWRGLLDRRKTPYVINPRQGYLFNWNNVPSAGWTAGDSEATERLAGPYHRS
                     VFLRRLVARVAKKPNYAASRAIDRTSGSTAQQRPLFGKRLRLARRGATGGAASVLDTL
                     LGWDGDYTRTDSRGTVPAGVATWEEFKDRAEAIALFALTRRSPGPGTVSLAGKTGTSH
                     EFDISNGEAYALRTLSPAALRLAAERTQAVLARRFGSATPSAWREPRRMYDVEIQGAA
                     DKPQMPFFDRGTWQQSVGLGP"
     gene            3236..3985
                     /locus_tag="VFY44_09465"
     CDS             3236..3985
                     /locus_tag="VFY44_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910709.1"
                     /translation="MPWVETESLSFVARHESGQTGSVERLLDDLERFRSALEPRYEIV
                     PGEVAVVVHPRSLMLSLAHPWLPAARMVSAPAGRRYFAGFYSRREIHVLAPAALEARA
                     SEVAGSREAMLLSPRHEYAHLVVGANNPSLPPPFTPGTFRRYVRMAWVSEGSATYLAG
                     QVPHMRAAVARRMREGGRPTFPPPARDALVLGGTVFDFLAREAGAEAAEQLALSRSPE
                     SALTALESALGRPAASIGRSWEDYLAGFAAG"
     gene            complement(3958..>4421)
                     /locus_tag="VFY44_09470"
     CDS             complement(3958..>4421)
                     /locus_tag="VFY44_09470"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX5910710.1"
                     /translation="DEELRTLRFAAIFHDIGKVSVRDDVLAKRGPLTSAERTEIEGHT
                     VVGEQILSSVDFLSEVLPLVRHEHERWDGGGYPDGLAGEAIPLGSRIVLACDAYDAMT
                     SDRPYRAAMTDAQARSELLAGAGTQFDERVVAALLQVLDEGAGQPAAKPAR"
BASE COUNT          579 a         1679 c         1612 g          551 t
ORIGIN      
        1 ggtcgagtgg gtcagcaaca taggcctgat cgcccgcgcg tcgctgcgcc tggcccagga
       61 ccggcccgcg gaggccgtcg ccgacctgcg ggcccaccgg gagatcgtcc agcgcttcgg
      121 ctggcgcgag ttcgcccgca gcttccccaa cgcgatgctg gccaccgcgc tggccgcggc
      181 gggccacaag gacgaggcgc gcgagctggc ccggcaggag gccgccttcg cccgcgacac
      241 cggccgccac agctgcctgg tcgcctcgct gacggcgctg ggccgggccc aggagggcaa
      301 ggaggccgtg gccacgctgg cggaggccgt ccgggcgtcc gcccattcgc agtcgccggt
      361 gatccgcgcc cgcgcgctgg tcgagctggg cgccgcgctg cgccgggacg ggcagcgggt
      421 cgacgcccgc gagcgcctgc gcgagggcgg cgagctggcc ggcacggccg gcgcgcgcct
      481 gttgcaggag caggcgctgg aggagctgtc catcgccggg gcgcgccagc gcgacccgtt
      541 cagctcgggc gtggacgcgc tcacgccgtc cgagcgccgg gtggccgagc acgcggcccg
      601 cggcatgacc aaccgcgaga tcgccgagac gctgttcgtg acccagaaga cgatcgaggt
      661 ccacctgggc aacacgtacg gcaagctcgg catccgctcg cgcaaccagc tccaggcggc
      721 gctcgaaggg cccgtgtcgg tgtaggttgg accccgctcg tggcgggggc ccgacgacat
      781 ccgtggctga cctgtgcgct cgcgctcggg atcctgtgcg cggggagcac cggcgcgcag
      841 gccaagatcc tgcaggccga gtccgtgctg ccgccgggcc agagcggcta cgtgtcggtc
      901 cccggggtgg tctcgggcac cgggtcgccc cacctggacg accagcgcac gctgttcacc
      961 tccttcggct tcaagagcgc gctgttcggc cagggcggcc agaccgagct gcccaaggcc
     1021 ggggtcaagg tcgtgcgcga ccgctacggc gtgcccgccg tgaccggcgc ctccgactac
     1081 gacgcgtggt ggggcgtggg ctacgcggtc gcccaggacc ggctgttcca gctggagctg
     1141 ttccgccgcg ccaccagcgg ccgcctggcc gagatcctgg gcgacggcta cctggacgac
     1201 gatctgatcg cgcgccgcga ctactacacc gacgccgagc tggacgcgat gctgggccgc
     1261 atcccggcgc agctcaggca gcgggccgag gcctaccgcg acggcatcaa cgcgtggtcg
     1321 tcgaaggtgc gctccgaccc ctcgctgctg ccgggcgagt tcgccgccct gggggccacg
     1381 ccgcccacct ggacgctgcg cgacaccgcg cgcgtgggcg tgttcctggc ccgcaccgtg
     1441 ccctccagcg acggccgcga gatcgacaac gcccgcgcgc tgcgcgagct gggccccaag
     1501 ggcttcgaca agctgctgcc gctgcggacc aagggcgcgc tgcccaccgt gcccgcctcc
     1561 agcgggcgct tcccgtccca gcccgggcgc tcggtcaggc aggagaaggc ggcgttcaag
     1621 cgctcgcagg gcgcgctgaa gggcgtgtcg ctgcccgagc cggcagcgtc ggcatcagca
     1681 tccgggacgc cccgttcgtc cgtcattcct catggcgggt cgtacatgtg ggccatcggc
     1741 cgccccgcca agaaggtccg caagtgccgc accgtcaagc gcaagggccg caaggccaag
     1801 cgccgctgca ggaccaccta ccggcgtccg aagaagcccg gcaacgccta cctgttcaac
     1861 gggccccagc tgggctactc ggtgcccgag ctgttcgtgg agtacgagct gcactcgccc
     1921 acccaggaca tccgcggcgt gtcggcggcg ggcatcccgg tgctgggcat cggccacaac
     1981 ggccacctgg cctggggctt cacctccggc ctgtcggacg aggacgacct gtacgccgag
     2041 aagctgaccg gcaccgaggc ctaccgctac aagggcgcct cgcgacccat ggactgccgc
     2101 accgagcggt tcgacttcaa ggcgccggtg accgacctgc ccgacctggt gggcacgccc
     2161 ggccggctgt cggggtcgcg caccgagcgg atctgccgca cgctgcacgg gccggtccag
     2221 gagcgcgccg gcggcaccgc ctacgcgcgc cggtacgcca tctggggccg cgagctggag
     2281 accctggtcg ggctgtcggc gctgaacgac gcgaagacgg tgcgccaggc cgacaccgcc
     2341 ctccagggcg tgacctggaa cgagaacgtg atggcggccg acgagcgcgg cgacatcggc
     2401 ttctggcacc cggggctgca cccgctccgg cccaagggct acgacgagcg cctgccctac
     2461 cccggcaccg gcgaggcgga gtggcgcggc ctgctggacc ggcgcaagac cccgtacgtg
     2521 atcaacccgc gccagggcta cctgttcaac tggaacaacg tcccgtcggc gggctggacc
     2581 gccggcgact ccgaggccac cgagcgcctg gccggcccgt accaccgctc ggtcttcctc
     2641 aggcgcctgg tggcccgggt ggcgaagaag ccgaactacg ccgcctcgcg cgccatcgac
     2701 cgcaccagcg gcagcaccgc gcaacagcgg ccgctgttcg gcaagcggct caggctcgcg
     2761 cgcaggggcg cgaccggcgg cgcggcctcc gtgctggaca cgctgctggg ctgggacggc
     2821 gactacaccc gcaccgactc ccgcgggacc gtgcccgcgg gcgtggccac ctgggaggag
     2881 ttcaaggacc gcgccgaggc catcgcgctc ttcgccctga ccaggcgctc gcccgggccg
     2941 ggcacggtgt cgctggccgg caagaccggc acctcgcacg agttcgacat ctccaacggc
     3001 gaggcctacg cgctgcgcac cctctccccc gccgccctgc gcctggccgc cgagcgcacc
     3061 caggccgtgc tggccaggcg cttcggcagc gccaccccgt ccgcctggcg cgaaccacgg
     3121 cgcatgtacg acgtcgagat ccagggcgcc gccgacaagc cccagatgcc gttcttcgac
     3181 cgcggcacct ggcagcagtc ggtcggactc ggtccctgac aggatcctcg gcccgatgcc
     3241 gtgggtcgag accgagagcc tgtcgttcgt agcccgtcac gagtcgggtc agacgggctc
     3301 ggtcgagcgg ctgctggacg acctcgagcg cttccgctcg gccctggagc cgcgctacga
     3361 gatcgtgccg ggcgaggtcg cggtggtcgt gcacccgcgg tcgctgatgc tgtcgctggc
     3421 ccacccctgg ctgcccgccg cgcggatggt gtcggccccg gccgggcggc ggtacttcgc
     3481 gggcttctac agccggcgcg agatccacgt gctggccccg gcggcgctcg aggcgcgtgc
     3541 ctcggaggtg gcgggctcac gcgaggccat gctgctctcg ccccggcacg agtacgcgca
     3601 cctggtggtg ggcgccaaca acccgtcgct gccgcccccg ttcacgcccg ggaccttccg
     3661 ccgctacgtg cgcatggcct gggtcagcga ggggtccgcc acctatctgg cgggccaggt
     3721 gccccacatg cgggcggccg tcgcgcggcg catgcgcgag ggcgggcggc ccacgttccc
     3781 gccgccggcg cgcgacgccc tggtgctggg cggcacggtg ttcgacttcc tggcccgcga
     3841 ggcgggcgcc gaggcggccg agcagctggc gctgagccgc agccccgagt cggcgctgac
     3901 cgcgctggag agcgcgctcg ggcggccggc ggcgtcgatc gggcgcagct gggaggacta
     3961 cctggcgggc ttcgccgcgg gctgacccgc gccctcgtcc agcacctgca ggagcgcggc
     4021 caccacgcgc tcgtcgaact gcgtcccggc cccggccagc agctccgacc gggcctgggc
     4081 gtcggtcatg gccgcccggt acgggcggtc cgaggtcatg gcgtcgtagg cgtcgcaggc
     4141 cagcacgatc cgtgagccca gcgggatcgc ctcgcccgcc agcccgtcgg ggtagccgcc
     4201 gccatcccag cgctcgtgct cgtggcggac cagcggaagc acctcggaca ggaagtccac
     4261 cgacgacagg atctgctcgc ccaccacggt gtggccctcg atctccgtgc gctcggcgga
     4321 ggtcagcggg ccgcgcttgg ccagcacgtc gtccctgacc gacaccttgc cgatgtcgtg
     4381 gaagatcgcg gcgaagcgca gcgtccgcag ctcctcgtcg c
//
LOCUS       DASUPW010000419         4261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_375142, whole genome shotgun sequence.
ACCESSION   DASUPW010000419 DASUPW010000000
VERSION     DASUPW010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375142"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1614
                     /locus_tag="VFY44_09475"
     CDS             <1..1614
                     /locus_tag="VFY44_09475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEX5910711.1"
                     /translation="GGGVTGAGAALDAASRGLKVALVEARDFAAGTSSRSSKLIHGGL
                     RYLEQLEFGLVREALRERSLLLNTIAPHLVRPVSFMMPLEHRVWERPYIGAGLVLYDG
                     LGGAKGLRRHRHLTKRGALRVAPGLKPDALIGAVQYQDCKVDDARHTMVVARTAAQYG
                     AAVASNAPVVGFLREAGRVTGVRVQDRQSGQEIEVAARQVINATGVWTDDVQDLVGER
                     GKFKVRASKGVHLVVPRDRVQLDTGLILRTEKSVLFVIPWGRHWIIGTTDNDWELDKA
                     HPAASASDIDYVLGHVNKVLASPLTHEDVEGVYAGLRPLLAEESEGTSKLSREHSVAS
                     PAPGLISVAGGKYTTYRVMGRDAVDAAVRGLDEKVPPSATQVTPLVGAEGYHVLWNQR
                     ARLARESGLHVERIEHLLGRYGSSIHELLDEVARDPELGKPLEGADDYLRVEVRYAAS
                     HEGAMHLDDVLARRTRISIETFDRGLASAEPAAALMGDVLGWSDEQRAQQVDLYNQRV
                     AAERDSQTKPDDQAADVARTSVPDFTAAS"
     gene            complement(1607..1999)
                     /locus_tag="VFY44_09480"
     CDS             complement(1607..1999)
                     /locus_tag="VFY44_09480"
                     /inference="COORDINATES: protein motif:HMM:NF020950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HEX5910712.1"
                     /translation="MRRLPAFAVLSLALLAPSAAQAKTVTVTKTAKNTTVKLEKGDVL
                     RVKLAENASTGYGWRRAVKPSSVLSYRGSRYVSPRQSDPQIVGAPGTRYFRFGAARRG
                     TTSFTLGLYPPGRGGKAAEKVKLKVAVS"
     gene            2050..2646
                     /locus_tag="VFY44_09485"
     CDS             2050..2646
                     /locus_tag="VFY44_09485"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX5910713.1"
                     /translation="MTEWAQEVLIAHGVAMLVLLILLENVFPPIPSEVILPLAGFLVF
                     EGQMTFVEAMVAATLGSVTGAVILYALGRHGGRPLLLRYGQVLRLDDRRLDRADDWFD
                     RHGPKIVFLGRMLPGVRSVVSVPAGLSEMRVPVFVALTALGSALWNAALIGAGWALGG
                     NWREVSEFVGPLTVPVILTVTLLAVAYALVRVRRARTE"
     gene            2643..3416
                     /gene="budA"
                     /locus_tag="VFY44_09490"
     CDS             2643..3416
                     /gene="budA"
                     /locus_tag="VFY44_09490"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEX5910714.1"
                     /translation="MSEHRFLDALRVDLLRHAELRPDRREHLLFQTSTIDALLSGHFD
                     GDVTIGELTGHGDLGLGTLNGCDGELVVVDGQAWQARLDGSLHRVPDAALTPYAVVTR
                     FDPGDHVELEGPLDFDALTARLDAAGRPGAQSAAVRIEGSFASMRVRSVPRQEPPYPP
                     LADVVAQQQVTDLAGVQGTVVGFRFPGPLEGIEIAGWHLHFVDAARARGGHVLGCVLD
                     RGTAALDHEADLHVELPPGVDPPRGGAASDSLLRRLERD"
     gene            complement(3413..>4261)
                     /locus_tag="VFY44_09495"
     CDS             complement(3413..>4261)
                     /locus_tag="VFY44_09495"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910715.1"
                     /translation="GGGWLQEGGTLDFAGGVPVEMGSGFSALAAALVVGARRDYGRAA
                     LLPHNAMYVLLGAGLLWFGWFGFNGGSGVSTGESGVLAFTNTLLTPACTLVVWFALDL
                     IRGRQVTAIGAATAIIVGCVAITPAGGFISPGWAMLLGAVAAVPSYAMILWRPRTRVD
                     ETLDVLAAHGIAGFTGILFIGVFASQAWNGVADGAAYGNLELLGDQALAAVAAPAYAF
                     GATYVLLRVMGALFPLRAGEREEALGMDVTQHGEEAYLTGEGAILVPRDNLVPADEKV
                     LAAAEA"
BASE COUNT          566 a         1572 c         1566 g          557 t
ORIGIN      
        1 gggggtgggg tgaccggtgc gggagccgcg ctggacgccg cctcccgagg gctgaaggtg
       61 gctctggtcg aggcgcgcga cttcgccgcc ggcacctcca gccggtccag caagctcatc
      121 cacggcggcc tgcgctacct cgagcagctg gagttcgggc tggtccgcga ggcgctgcgc
      181 gagcgcagcc tgctgctgaa caccatcgcc ccccatctag tccgcccggt ctcgttcatg
      241 atgccgctgg agcaccgcgt ctgggagcgg ccgtacatcg gcgccggcct ggtgctgtac
      301 gacggcctcg gcggcgccaa gggcctgcgt cgccaccgcc acctgaccaa gcgcggcgcg
      361 cttcgggtgg cgcccggcct caagcccgat gccctgatcg gcgcggtcca gtaccaggac
      421 tgcaaggtcg acgacgcccg tcacacgatg gtcgtggcgc ggacggcggc ccagtacggc
      481 gccgccgtgg ccagcaacgc cccggtggtg ggtttcctgc gcgaggcggg acgcgtgacc
      541 ggcgtgcgcg tccaggaccg acagagcggc caggagatcg aggtcgccgc gcgccaggtg
      601 atcaacgcca ccggcgtctg gaccgacgac gtccaggacc tggtcggcga gcgcggcaag
      661 ttcaaggtgc gggcctcgaa gggcgtccac ctggtggtcc cgcgtgaccg ggtgcagctg
      721 gacaccggcc tgatcctgcg caccgagaag tcggtgctgt tcgtgatccc ctgggggcgg
      781 cactggatca tcggcaccac cgacaacgac tgggagctgg acaaggccca tccggccgcg
      841 tcggccagcg acatcgacta cgtgctcggg cacgtgaaca aggtgctggc cagcccgctg
      901 acccacgagg acgtggaggg cgtgtacgcc ggcctgcgcc cgctgctggc cgaggagagc
      961 gagggcacca gcaagctcag ccgcgagcac tcggtggcct cccccgcgcc gggcctgatc
     1021 tcggtggccg gcggcaagta cacgacgtac cgggtcatgg gccgtgacgc cgtggacgcg
     1081 gccgtccgcg ggctggacga gaaggtgccc ccgtcggcga cccaggtcac gccgctggtg
     1141 ggcgccgagg gctaccacgt gctgtggaac cagcgcgcgc ggctggcgcg cgagagcggc
     1201 ctccacgtgg agcgcatcga gcatctgctg ggccggtacg gctcgtccat ccacgagctg
     1261 ctggacgagg tcgcgcgcga cccggagctg ggcaagccgc tggagggcgc ggacgactac
     1321 ctgcgcgtgg aggtgcgcta cgccgcgtca cacgagggcg cgatgcacct ggacgacgtg
     1381 ctggcgcggc gcacccggat ctcgatcgag acgttcgacc gcgggctggc ctcggccgag
     1441 ccggcggccg cgctgatggg cgatgtcctg ggctggagcg acgagcagcg cgcgcagcag
     1501 gtcgacctct acaaccagcg cgtggcggcc gagcgcgact cgcagaccaa gcccgacgac
     1561 caggccgccg acgtggcgcg cacgtcggtg ccggacttca ccgccgctag ctgacggcga
     1621 ccttgagctt gaccttctcg gccgccttgc cgccgcggcc gggcgggtag aggccgagcg
     1681 tgaacgaggt ggtgccccgc cgggccgccc cgaaccggaa gtagcgcgtg cccggcgcgc
     1741 ccacgatctg cgggtcgctc tggcgcggcg agacgtacct gctgccccgg taggagagca
     1801 ccgaggacgg cttgaccgcg cgccgccagc cgtagccggt gctggcgttc tcggccagct
     1861 tgacgcgcag cacgtcgccc ttctccagct tgaccgtggt gttcttcgcc gtcttggtca
     1921 cggtcaccgt cttggcctgg gccgcagagg gagccagcag ggcgagggac aggacggcga
     1981 aggcgggcag tcggcgcatc gggcgacgct acacccgcat ccgcgcccgc gccagctacc
     2041 gtgcgcgcgg tgaccgaatg ggcacaagag gttctgatcg cgcacggcgt ggcgatgctg
     2101 gtgctgctga tcctgctcga gaacgtgttc ccgccgatcc cgtcggaggt catcctcccc
     2161 ctggccgggt tcctggtgtt cgagggccag atgacgttcg tcgaggccat ggtggccgcg
     2221 accctgggct cggtgacggg ggccgtgatc ctgtacgcgc tcggccgcca cggtggccgg
     2281 ccgctgctgc tgcgctacgg ccaggtcctg cgcctggacg accgccggct cgaccgcgcc
     2341 gacgactggt tcgaccgcca cggccccaag atcgtgttcc tgggccggat gctgcccggc
     2401 gtgcgcagcg tggtgtcggt gcccgccggg ctgtcggaga tgcgcgtgcc ggtgttcgtg
     2461 gccctgaccg cgctgggctc ggcgctgtgg aacgcggcgc tgatcggcgc cggctgggcg
     2521 ctgggcggca actggcgcga ggtcagcgag ttcgtcgggc ccctgaccgt gcccgtgatc
     2581 ctcaccgtga ccctgctcgc ggtggcctac gcgctggtgc gcgtgcggcg cgcgcgcacc
     2641 gagtgagcga gcaccggttc ctcgacgcgc tccgcgtcga cctgctgcgc cacgccgagc
     2701 tgcgccccga ccggcgcgag cacctgctgt tccagacctc gaccatcgat gcgctgctgt
     2761 ccggccactt cgacggcgac gtcacgatcg gcgagctgac cggccacggg gacctggggc
     2821 tgggcacgct caacggctgc gacggtgaac tggtggtggt cgacggccag gcgtggcagg
     2881 cgcgtctgga cggctccctg caccgcgtgc ccgacgccgc gctgacgccg tacgcggtgg
     2941 tcacccggtt cgaccccggc gaccacgtcg agctggaggg gccgctggac ttcgacgccc
     3001 tgaccgcccg cctggacgcc gccgggcggc cgggagccca gagcgcggcc gtgcgcatcg
     3061 agggcagctt cgcgtccatg cgggtccgct cggtgccgcg ccaggagccg ccgtacccgc
     3121 cgctggccga cgtggtggcc cagcagcagg tgaccgacct ggccggcgtg cagggcaccg
     3181 tggtgggctt ccggttcccc ggcccgctcg aggggatcga gatcgccggc tggcacctgc
     3241 acttcgtgga cgctgcccgc gcccgcggcg ggcacgtgct tgggtgcgtg ctcgaccgcg
     3301 ggacggccgc gctggaccac gaggcggacc tccacgtcga gctgccgccc ggtgtggacc
     3361 cgccccgcgg cggcgccgcg agcgactcgc tgctgcgccg gctcgagcgc gactaggcct
     3421 cggccgccgc cagcaccttc tcgtcggccg gcacgaggtt gtcgcgcggc accaggatcg
     3481 ccccctcccc cgtcaggtag gcctcctcgc cgtgctgggt cacgtccatg cccagcgcct
     3541 cctcgcgctc gccggcgcgg agcgggaaca gcgcgcccat cacgcgcagc agcacgtagg
     3601 tggcgccgaa cgcgtacgcc ggcgccgcca cggccgccag cgcctggtca cccagcagct
     3661 ccaggttgcc gtaggcggcc ccgtcggcca cgccgttcca ggcctggctg gcgaacaccc
     3721 cgatgaacag gatccccgtg aagcccgcga tgccgtgcgc ggccagcacg tccagcgtct
     3781 cgtccacgcg cgtgcgcggg cgccagagga tcatggcgta gctgggcacg gccgccacgg
     3841 cgcccagcag catcgcccag ccggggctga tgaagccgcc cgccggggtg atggccacac
     3901 agccgacgat gatcgccgtg gccgcgccga tggccgtgac ctgccggccc cggatcaggt
     3961 ccagcgcgaa ccagaccacc agcgtgcagg ccggcgtcag cagcgtgttg gtgaacgcca
     4021 gcacgcccga ctcgccggtg gacacgccgc tgccgccgtt gaagccgaac cagccgaacc
     4081 acagcagccc cgcacccagc agcacgtaca tggcgttgtg cggcagcagc gccgcccggc
     4141 cgtagtcgcg ccgcgcaccc accaccagcg ccgcggccaa cgccgagaag cccgacccca
     4201 tctccaccgg cacgccgccg gcgaagtcca gcgtgccgcc ctcctggagc cagccgccgc
     4261 c
//
LOCUS       DASUPW010000420         2273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_375269, whole genome shotgun sequence.
ACCESSION   DASUPW010000420 DASUPW010000000
VERSION     DASUPW010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375269"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VFY44_09500"
     CDS             <1..339
                     /locus_tag="VFY44_09500"
                     /inference="COORDINATES: protein motif:HMM:NF022793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3151 domain-containing protein"
                     /protein_id="HEX5910716.1"
                     /translation="TAGNPPAEVASRHPTVSAAWAALAEEALTRSERELSDAVEAYAY
                     ARTGYHRGLDALRRNGWKGFGPVPWSHEPNRGFLRCVIALAKAAEAIGETEEQERLTQ
                     LLRDSDPTLA"
     gene            complement(336..1142)
                     /locus_tag="VFY44_09505"
     CDS             complement(336..1142)
                     /locus_tag="VFY44_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5910717.1"
                     /translation="MTPAALPVLELRGVTVRFGAVAALSRVSSVLPAGEVTCVLGENG
                     SGKSTLVALLSGLTRHTEGTLLVSGEPMRFRSPRQARAAGIATVWQDLAVAPLLPVWR
                     NFFLGAEPSRGVGPFRRLDLDRARTTTVSALARVGVTGLDPDAPASTLRAGERQSLAI
                     ARALHFGARALVIDEPTAPLAVPQQTLMLQSIVTARSQGLAVLQVTNTPRYAHLVGDR
                     FLLLAHGQVAGSLTRDDVDASDLTRLMAGGDEFSALTAALTALHPEIGER"
     gene            complement(1139..2221)
                     /locus_tag="VFY44_09510"
     CDS             complement(1139..2221)
                     /locus_tag="VFY44_09510"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5910718.1"
                     /translation="MPVITERLPALGSVPADAPRTRRWFVRFLARPSLPALVGLAAVL
                     AVFLPLAPQLLTVAGLASVLDSAALIGIGGVTVGLLLIAGHFDLSIGVVAVASALGTA
                     LLTGQAGWAVWPALLVSFVGALLIGLFNGLLVVNTGLPSFLVTLATFLVLQGIARAGG
                     TAVSGVSRVTGLEEAEGWQSVAAVFGSTLEIGDGRFRISLVWFLLVTAVATWALWRTG
                     FGNGIFASGGARRAARQLGVPVARTTVVLFCVTATAGWLIGTLGLVRLAGVQVSGVQI
                     GSTGLISAIDFIVVAVIGGCLLTGGYGSTIGAAIGAVVYAVARNGIVMAGWDPLWFQA
                     LLGVLLLTALLTNGVVRRRLKAAPRS"
BASE COUNT          349 a          835 c          866 g          223 t
ORIGIN      
        1 accgccggca acccgcccgc cgaggtcgcg tcgcgccacc ccacggtcag cgcggcctgg
       61 gcggcgctgg ccgaggaggc gctgacccgg tcggagcggg agctcagcga cgccgtcgag
      121 gcctacgcct acgcccgcac cggttaccac cgcgggctgg acgccctgcg tcgcaacggc
      181 tggaagggct tcggcccggt gccgtggtcg cacgagccca accgcgggtt cctgcgctgc
      241 gtcatcgcgc tcgcgaaggc cgccgaggcg atcggcgaga ccgaggagca ggagcgcctg
      301 acgcagctcc tccgcgacag cgacccgacc ctggcttagc gctcgccgat ctcggggtgc
      361 agggcggtca gggcggcggt gagcgcggag aactcgtcgc cgccggccat cagccgggtg
      421 aggtcggagg cgtcgacgtc gtcccgggtc aggctgcccg ccacctggcc gtgcgccagc
      481 agcaggaacc ggtcgccgac caggtgggcg tagcgcgggg tgttggtcac ctgcagcacg
      541 gccaggccct ggctgcgggc ggtgacgatc gactgcagca tcagcgtctg ctggggaacg
      601 gcgagcggcg ccgtgggctc gtcgatgacc agcgcgcgag cgccgaagtg cagggcgcgg
      661 gcgatggcca ggctctgccg ctcgccggcg cgcagcgtgc tcgcgggtgc gtccgggtcc
      721 agcccggtca cgccgacgcg ggcgagggcg gagacggtcg tcgtccgggc gcggtcgagg
      781 tccagccggc ggaacgggcc gaccccgcgg gacggctcgg cgccgaggaa gaagttccgc
      841 cacaccggca gcagcggcgc gacggcgagg tcctgccaca cggtcgcgat gccggcggcg
      901 cgggcctggc gcggcgatcg gaagcgcatc ggctcgccgc tcaccaggag cgtgccctcg
      961 gtgtgccggg tgagccccga caggagcgcc accagcgtcg acttgcccga cccgttctct
     1021 ccgagcacgc aggtgacctc accggcgggg aggacggagc tgacgcggga gagcgcggcg
     1081 acggccccga agcgcacggt caccccgcgg agttcgagca ccggcagcgc tgcgggggtc
     1141 atgaccgcgg tgccgccttc agccgccggc ggacgacgcc gttggtgagc agggcggtca
     1201 gcagcaggac gccgagcagc gcctggaacc acagcgggtc ccacccggcc atgacgatgc
     1261 cgttgcgggc caccgcgtag accacggcgc cgatcgccgc gccgatcgtc gaaccgtagc
     1321 cgcctgtcag caggcagccg ccgatcaccg cgacgacgat gaagtcgatg gcgctgatca
     1381 gcccggtcga gccgatctgc acgccgctca cctgcacgcc cgcgagacgg accaggccca
     1441 gggtgccgat cagccagccc gccgtcgcgg tgacgcagaa caggacgacc gtcgtccggg
     1501 ccaccggcac gccgagctgc cgggccgccc ggcgcgcccc gccgctggcg aagatgccgt
     1561 tgccgaagcc ggtccgccac aacgcccagg tcgccaccgc cgtgaccagc aggaaccaga
     1621 ccagcgagat ccggaagcga ccgtcgccga tctcgagggt ggagccgaac acggccgcca
     1681 ccgactgcca accttcggcc tcctccagcc ccgtcacccg ggagactccg ctgactgccg
     1741 tgccgccggc gcgcgcgatg ccctggagca ccaggaacgt cgcgagggtg accaggaagc
     1801 tgggcagccc ggtgttgacg acgaggagcc cgttgaacag cccgatgagc agggccccga
     1861 cgaacgagac cagcagcgcc ggccacacgg cccagccggc ctgcccggtg agcagggcgg
     1921 tgccgagcgc gctggcgacg gccaccacgc cgatcgacag gtcgaagtgc ccggcgatca
     1981 gcagcaggcc gacggtcacg ccgccgatcc cgatcagggc cgcggagtcc aggacgctgg
     2041 ccagacctgc gacggtgagc agctgcggcg ccagcgggag gaagacggcg agcaccgccg
     2101 ccaggcccac cagggccggc aggctgggcc gcgccaggaa ccgcacgaac cagcgccggg
     2161 tccgcggcgc gtctgcggga accgagccca gtgcgggcag gcgctcggtg atcactggca
     2221 cgtcccgaga gcggtccggg gaccggacgg ggcgcggagc gtcgccagcg ggg
//
LOCUS       DASUPW010000421         3011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_96866, whole genome shotgun sequence.
ACCESSION   DASUPW010000421 DASUPW010000000
VERSION     DASUPW010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96866"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..171)
                     /locus_tag="VFY44_09515"
     CDS             complement(<1..171)
                     /locus_tag="VFY44_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019394039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL13 family ribosomal protein"
                     /protein_id="HEX5910719.1"
                     /translation="MKTFVATPENRQRDWIVVDAAGQTLGRLATQIADRLRGKYKPEY
                     TPHCDVGDFVIVI"
     regulatory      313..479
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            531..1715
                     /locus_tag="VFY44_09520"
     CDS             531..1715
                     /locus_tag="VFY44_09520"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX5910720.1"
                     /translation="MRATGAALACLCAGLAAAPGAAGAQTGGGAQFEESPAVKKVACV
                     KACAKRKRPRAGSTLRLRGSGLSGVTTVIFQGVPGAEDDVRAPVTRKSAASAWVVVPA
                     GAASGPLVALNQTGRRSKASAIVSLLPPPPPSPNPTLTPVPGVRQDGAPALETGTSRV
                     KVFFGARRAVTFSYRVTDGVASGLQVQLVRVADGSVVRTWAPAPPALGTVASVVWNGD
                     LAGKKAAPAGRYAFRLTASAGSGAVARSAQTSNVTRDSFDLYQDQFPIRGPHKYEGGL
                     GDGRGHQGQDIFAKCGTPLVAARGGKVKTKAYQSRAGNYLVIDGDGTGNDYAYMHMAA
                     PSPFEEGDRVYTGQQIGQVGQTGRASGCHLHFEAWSAPGWYSGGRVMNPTPLLKAWDA
                     YS"
     gene            1745..2053
                     /locus_tag="VFY44_09525"
     CDS             1745..2053
                     /locus_tag="VFY44_09525"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5910721.1"
                     /translation="MADLQRSIDFYETLGFEVAERYEQEGWLLWVRLRRGSAELMLAL
                     ASEPVHDHAQGVLFYLYVDDLDAIEADAPVLEHEAPRPAREMRLTDPDGYVVMVAELA
                     "
     gene            2050..2871
                     /locus_tag="VFY44_09530"
     CDS             2050..2871
                     /locus_tag="VFY44_09530"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5910722.1"
                     /translation="MSSLKAFEADGWNRQAEGYDLLTGRVTARVAPPLLDAADVMAGH
                     RVLDVACGTGALSAAATRRGASPLGIDLAEAMVDAARSGLPGVEFRVADAEALPFPDD
                     RFDAAVGGFVLNHLPHPARCAEECARVVAPGGRVAFAVWERPERSRLTALLGEALEHA
                     GGDRGAGVPEGPDDFRYADAGRMRRLLEGAGLEEVDAATVDLDVRVADAGELWRGLTA
                     GLVRAPAALAANDQDTRDRARAEFDDLVREFAVADGGLSVPAVVRVGAGRVRLEP"
BASE COUNT          379 a         1141 c         1107 g          384 t
ORIGIN      
        1 gatcacgatc acgaagtcgc cgacatcgca gtgcggcgtg tactccggct tgtacttgcc
       61 ccgcagccgg tcggcgatct gggtggccag ccggccgagg gtctgacccg cggcgtcgac
      121 gacgatccag tctcgctggc ggttctccgg ggtggcgacg aaggtcttca ttacgtacaa
      181 gcggccgcgc ggatggcggc cggtcgaaca gtgtggcaga tgagcgctgg atcgtccctg
      241 cagcctggcg ccggcacgcg cgaaatgccg tttcgcgtta cgccgtcggc ctggtgggta
      301 tgatcgtcgc gattcgaacg cctgccgaat cccggtggcg ccgccaggtg tcacggggaa
      361 cgggggaacc gatggagccc gtcgccgggc tccaaggggc gaatcggcaa tgacgccgta
      421 gcgctgctct ttcagcgcga gcccgacagc taaccccgta ggcccgagaa agagaggcag
      481 tgcccgtgcc gaggaacccc tcctcagcgc acaaccggtc gtacagccgt atgcgcgcca
      541 ccggcgccgc cctggcctgc ctgtgcgcgg gcctggccgc ggcccccggc gcggccggcg
      601 cccagaccgg cggcggcgcc cagttcgagg agtccccggc ggtcaagaag gtcgcctgcg
      661 tcaaggcgtg cgccaagcgc aagcgccccc gggccggatc gacgctgcgc ctgcgtggca
      721 gcggcctctc gggcgtgacc accgtgatct tccagggcgt gcccggcgcc gaggacgacg
      781 tcagggcgcc cgtcacccgc aagtcggccg cgagcgcgtg ggtcgtggtc cccgccggcg
      841 ccgcctccgg cccgctggtg gcgctgaacc agaccggccg ccgctcgaag gcgtcggcca
      901 tcgtctcgct gctgccgcct cccccgccca gcccgaaccc gacgctgacg ccggtgcccg
      961 gcgtgcgcca ggacggcgca cccgcgctgg agaccggcac cagccgggtc aaggtcttct
     1021 tcggcgcccg ccgcgccgtc acgttctcct accgcgtgac cgacggcgtg gcctccggcc
     1081 tgcaggtgca gctggtgcgc gtggccgacg gctcggtggt ccgcacctgg gcgccggccc
     1141 cgcccgccct gggcacggtg gccagcgtgg tctggaacgg cgacctggcc ggcaagaagg
     1201 ccgcgcccgc cgggcgctac gccttccgcc tgaccgcctc cgccggctcc ggcgccgtgg
     1261 cccgcagcgc gcagacgtcc aacgtcacgc gtgactcgtt cgacctctac caggaccagt
     1321 tccccatccg cgggccgcac aagtacgagg gcggcctggg cgacgggcgc ggccaccagg
     1381 gccaggacat cttcgccaag tgcggcaccc cgctggtggc ggcccgcggc ggcaaggtca
     1441 agaccaaggc ctaccagtcg cgcgcgggca actacctggt catcgacggc gacggcacgg
     1501 gcaacgacta cgcctacatg cacatggccg ccccgtcgcc cttcgaggag ggcgaccgcg
     1561 tctacaccgg ccagcagatc ggccaggtgg gccagaccgg ccgcgccagc ggctgccacc
     1621 tgcacttcga ggcctggagc gcgcccgggt ggtactccgg cgggcgcgtg atgaacccca
     1681 cgccgctgct gaaggcctgg gacgcctact cctgagccgt tcggcgctcg tcccgctggc
     1741 ccacgtggcc gacctgcaac ggtcgatcga cttctacgag acgctgggct tcgaggtcgc
     1801 cgagcgctat gagcaggagg gttggctgct gtgggtccgc ctgcgccgcg ggtcggccga
     1861 gctgatgctg gcgctggcgt ccgagccggt gcacgatcat gcgcagggcg tgctcttcta
     1921 cctgtacgtg gacgacctgg acgccatcga ggccgacgcc ccggtgctcg agcacgaggc
     1981 gccccgcccg gcgcgcgaga tgcgcctgac cgaccccgac ggctacgtgg tcatggtcgc
     2041 cgagctggca tgagctcgct gaaggcgttc gaggccgacg gctggaaccg ccaggccgag
     2101 ggctacgacc ttctgaccgg ccgcgtgacc gcgcgcgtgg cccctcccct gctggacgcc
     2161 gccgacgtga tggcgggcca ccgcgtgctg gacgtggcct gcggcaccgg ggcgctgtcg
     2221 gccgccgcca cgcgccgcgg ggcctcgccg ctggggatcg acctggccga ggccatggtc
     2281 gacgccgccc ggagcgggct cccgggggtc gagttccgcg tggccgacgc cgaggcgctg
     2341 cccttccccg acgaccgctt cgacgccgcc gtcggcggct tcgtgctgaa ccacctgccc
     2401 caccccgccc gctgcgcgga ggagtgcgcg cgcgtggtgg ccccaggcgg gcgcgtggcc
     2461 ttcgccgtct gggagcgccc ggagcgctcg cgcctgaccg ccctgctggg cgaggccctg
     2521 gagcacgcgg gcggcgaccg cggcgccggc gtgcccgagg ggcccgacga cttccgctac
     2581 gccgacgccg gccgcatgcg ccgcctgctg gagggcgccg gcctcgagga ggtcgacgcg
     2641 gcgaccgtgg acctggacgt gcgggtcgcc gatgccggcg agctgtggcg cggcctgacc
     2701 gccggcctgg tgcgcgcccc ggccgcgctc gcggccaacg accaggacac gcgcgaccgt
     2761 gcgcgggccg agttcgacga tctggtgcgc gagttcgcgg tcgccgacgg cggcctcagc
     2821 gtccccgccg tcgtgcgcgt gggcgccgga cgagtcagac tcgagccatg accgccgtcg
     2881 gggaggagct cgcccgcgcc atcgccgcca aggacgcggc cgcgctgcgc gagctgctgc
     2941 ggcccgacgt cgacttcacc gcgatcaccc cgcgccggca ctggaacgcc gggacgccgg
     3001 acgacgtcgc c
//
LOCUS       DASUPW010000422         3769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1482152, whole genome shotgun sequence.
ACCESSION   DASUPW010000422 DASUPW010000000
VERSION     DASUPW010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482152"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VFY44_09535"
     CDS             complement(<1..761)
                     /locus_tag="VFY44_09535"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5910723.1"
                     /translation="MRLRREARWLWPILAIVLTGLACAAYVLNKQRLESPLADRYELL
                     LEFDNADAVTPGTGSPITVAGVKVGQIDGARLEGGRGVVSVSIDPAELPHVYADATAQ
                     LVPNTPLKDMQIRLYPGRRSAGRLRDGGRIDVADTITPVDADGFRRALDSDTRAWVRS
                     LIAGLDVGLKGRKRDLNRALRSLGPTAAQLRRISSLLASRRKTIPALVHNLRVITEAT
                     ASRDRDLTRVVHAGDATFDALARNDASLRRALELLP"
     gene            complement(758..2239)
                     /locus_tag="VFY44_09540"
     CDS             complement(758..2239)
                     /locus_tag="VFY44_09540"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5910724.1"
                     /translation="MRAPGAVPLKPYAIAVGLIVGLVLVTWFSFNPRLPFSSGYRVHA
                     VFQSSNGLRDGSPVRVAGITVGAVVDIGEGPGNTTVVTIELKDSARPVRRDATVRIRP
                     RVFLEGGFLAELQPGSPSAPELRDGGTIPLAQTSIPVQFHQTMDVLDHPGRQALRTSL
                     DAVAGGLADGGADGLRTLAPELRPLLRDTAWFAEAARGTRPDDVSRLVRATSRVAAAL
                     DRPPERLGSLVDNLAVTASAVRSRDTQLSASIAELNGVLRATPAAVRALDAGLPALER
                     AGRHVGPALPIAPRAFRDTTAAVRSLGRLTAPGRRARTIAALETAFRDVPGLVGRLAR
                     AFPDAKPLADCLSSHVLPLFNAKVPDGALSTGRPVWQDLVHSTVGLAGASQNFDANGP
                     TVRYQLGIGGQSLAAFPGAGELLASAPGTLRSRPLPRPDRTFPPLNSRAPCSRQPQPV
                     LATPSGAAGFQPLATPRATTKRVEMKELLDPESLERVMRGDRP"
     gene            complement(2236..3624)
                     /locus_tag="VFY44_09545"
     CDS             complement(2236..3624)
                     /locus_tag="VFY44_09545"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5910725.1"
                     /translation="MTVRGPRATRRRWYADMRIGGALILAVAAALMYVSYTAQNGLPW
                     EDTYDVRIAVPDAGKLIKNTDVKVGGARVGQVLGIEAVPRRGDVPPHAVLDVQLEEDA
                     GPLAVDSTAEVRLASVLGGKYVELVPGKARRVIPEGGTLPLANSATTVEIEDALAVFD
                     PEGRAALQRVIASLGDALAGRGGDLNETIGTTASMLPRLEAVLSNLSAEETRLGAFVR
                     GAAAATTALEPVAGELGPLVRASGDTLGALDAAGASLGESIEEFPPAARATRRALRRL
                     NPVLDDTVAVSRELRPAAAVLGPSARGIRGVAATAIRLDPELGRMARPVDRLLGGVDA
                     FSRNPETSRALRLLGSTDLATFGASATLGLGALLETTWDAEQHCRVASTWIRRLVDIT
                     SDGNAGGNWVRMAPLDEPTQMVPQGRPSANLHANPYPNADARECEAGNEGFAPGQMIG
                     NPPGIQGGRSGP"
BASE COUNT          442 a         1409 c         1459 g          459 t
ORIGIN      
        1 gggagcagct ccagcgcgcg ccggagcgat gcgtcgttgc gggccagggc gtcgaacgtg
       61 gcgtcgcccg cgtggaccac ccgggtcagg tcccggtcgc ggctggccgt ggcctcggtg
      121 atcacgcgca ggttgtgcac cagggcgggg atcgtcttgc gacggctggc cagcaggctg
      181 gagatccggc gcagctgcgc cgccgtcggc ccgaggctgc gcagcgcccg gttgaggtcg
      241 cgcttgcgcc ccttgaggcc cacgtcgagc ccggcgatca ggctccgcac ccacgcccgc
      301 gtgtccgagt ccagcgcgcg ccggaagccg tcggcgtcga ccggcgtgat ggtgtccgcc
      361 acgtcgatcc ggccgccgtc gcgcaggcgc ccggccgagc gccggccggg gtagaggcgg
      421 atctgcatgt ccttgagcgg ggtgttgggc accagctgcg cggtcgcgtc ggcgtacacg
      481 tggggcagct ccgcggggtc gatgctcacg ctgaccaccc cgcggccgcc ctccagcctg
      541 gccccgtcga tctggccgac cttcaccccc gccacggtga tcggcgagcc cgtgccgggg
      601 gtcacggcgt ccgcgttgtc gaactccagc aggagctcgt accggtcggc gagcggcgac
      661 tccagccgct gcttgttcag cacgtaggcg gcacaggcca ggccggtgag cacgatggcc
      721 aggatcggcc acagccagcg ggcctcccgt cgcagcctca cgggcggtcc ccccgcatca
      781 cgcgctccag gctctcgggg tcgagaagct ccttcatctc gacgcgcttg gtggtcgcgc
      841 ggggtgtcgc cagcggctgg aacccggccg cgccgctggg tgtggccagc acgggctgcg
      901 gctggcgcga gcacggggcg cggctgttca gcggcgggaa cgtgcggtcg ggccggggga
      961 ggggtcgcga gcgcagcgtg cccggagcgg aggccagcag ctcccccgcc cccgggaagg
     1021 cggccagcga ctgcccgcca atgcccagct ggtagcggac cgtcggcccg ttggcgtcga
     1081 agttctgcga cgcgccggcc aggccgaccg tcgagtgcac caggtcctgc cacaccggcc
     1141 ggcccgtgga cagcgcgccg tcgggcacct tcgcgttgaa cagcggcagc acgtgcgagc
     1201 tcaggcagtc ggccagcggc ttggcgtcgg ggaacgcccg ggccagccgc ccgaccagcc
     1261 cgggcacgtc gcggaacgcg gtctccaggg ccgcgatcgt ccgcgcgcgc cggcccgggg
     1321 ccgtgagccg gcccagcgag cggacggcgg ccgtggtgtc gcggaaggcc cgcggggcga
     1381 tcggcagggc ggggcccacg tgccggccgg cgcgctccag ggccggcagc ccggcgtcca
     1441 gggcccgaac ggccgccggg gtggcgcgca ggacgccgtt gagctcggcg atcgaggcgg
     1501 acagctgggt gtcgcgcgag cggaccgcgg acgccgtgac ggccaggttg tcgaccaggg
     1561 accccaggcg ctcggggggg cggtccagcg cggccgcgac cctgctggtg gcccgcacca
     1621 ggcgggacac gtcgtccggc cgcgtccccc gcgcggcctc ggcgaaccac gccgtgtcgc
     1681 ggaggagggg gcgcagctcg ggcgcgaggg tgcgcagtcc gtccgcgccc ccgtccgcca
     1741 ggccgccggc caccgcgtcg aggctcgtgc gaagggcctg gcgcccgggg tggtcgagca
     1801 cgtccatcgt ctggtggaac tggaccggga tcgaggtctg cgccagcggg atggtgccgc
     1861 cgtcgcgcag ctccggcgcg ctggggctcc cgggctgcag ctcggccagg aagccgccct
     1921 cgaggaacac ccgcggccgg atccgcaccg tggcgtcgcg gcggacgggg cgcgcgctgt
     1981 ccttgagctc gatcgtgacc acggtggtgt tgcccggccc ctcgccgatg tcgaccacgg
     2041 cgcccacggt gatgccggcc acgcgcacgg gggacccgtc gcgcagcccg ttcgagctct
     2101 ggaacaccgc gtggacgcgg tagcccgatg agaacggcag cctggggttg aagctgaacc
     2161 aggtcaccag gaccagcccc acgatcaggc ccaccgcgat ggcgtagggc ttcagcggca
     2221 cggccccggg cgccctcacg gcccgctcct cccaccctgg atgccgggcg ggttgccgat
     2281 catctggccg ggggcgaagc cctcgttgcc cgcttcgcac tcccgcgcgt cggcgttcgg
     2341 gtacgggttg gcgtgcaggt tcgccgacgg ccggccctgg ggcaccatct gcgtcggctc
     2401 gtccaacggg gccatgcgca cccagttgcc gccggcgttg ccgtcgctgg tgatgtccac
     2461 cagccgccgg atccaggtgg aggccacgcg gcagtgctgc tcggcatccc aggtcgtctc
     2521 cagcagcgcg cccaacccca gcgtggccga ggcgccgaac gtggccaggt ccgtggagcc
     2581 cagcagccgc agcgccctgc tggtctccgg gttgcgcgag aaggcgtcga cgcctcccag
     2641 gagccggtcc accgggcgcg ccatgcgccc cagctcgggg tcgagccgga tcgcggtggc
     2701 cgccacgccg cggatgccgc gcgcgctggg gcccaggacc gcggccgcgg gccggagctc
     2761 gcgcgagacg gccacggtgt cgtccagcac ggggttgagc ctgcgcagcg cgcgccgcgt
     2821 ggcgcgggcg gccggcggga actcctcgat gctctcgccg agcgacgccc cggcggcgtc
     2881 cagtgccccc agcgtgtccc cgctcgcgcg gaccagcggg cccagctcgc ccgcgaccgg
     2941 ctcgagcgcc gtggttgccg cggccgctcc gcgcacgaag gcaccgaggc gtgtctcctc
     3001 ggccgacagg ttggacagca cggcctcgag ccggggcaac atcgaggccg tggtgccgat
     3061 cgtctcgttc aggtcgccgc cgcggccggc caacgcgtcg cccagcgagg cgatcacccg
     3121 ctgcagggcg gcgcgcccct ccgggtcgaa cacggccagg gcgtcctcga tctcgacggt
     3181 ggtcgccgag ttggccaggg gcagcgtccc gccctcgggg atgacccggc gggccttgcc
     3241 gggcacgagc tccacgtact tgccgcccag gacggaggcc aggcgcacct cggcggtgga
     3301 gtccaccgcc aggggacccg cgtcctcctc gagctggacg tccagcaccg cgtgcggggg
     3361 cacgtcaccc cggcgcggca ccgcctcgat gcccagcacc tggcccaccc gggcgccgcc
     3421 gaccttgacg tcggtgttct tgatcagctt gccggcgtcg ggcacggcga tccggacgtc
     3481 gtatgtgtcc tcccagggca gcccgttctg cgccgtgtag gagacgtaca tcagcgcggc
     3541 ggcgaccgcg aggatcagcg cgcccccgat ccgcatgtcc gcgtaccagc gccggcgcgt
     3601 cgctctgggc ccccgcaccg tcatcgcagg aggtagtcca ggagggggac gtcgggcggg
     3661 ccgtccggcg cgggcgtcgt ggcgggcggg gcggcggtga cggacgcgcg cttgggcgag
     3721 ggctcgcccg cgtccttcga ggggccgccc gacgtgacgc ccgagcccg
//
LOCUS       DASUPW010000423         3474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_236507, whole genome shotgun sequence.
ACCESSION   DASUPW010000423 DASUPW010000000
VERSION     DASUPW010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236507"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..1143
                     /locus_tag="VFY44_09550"
     CDS             196..1143
                     /locus_tag="VFY44_09550"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX5910726.1"
                     /translation="MKVLSIDGGGIRGLIPAVVLAEIERRSGRRIADLFDLIAGTSTG
                     GILAAALACPDGDGRPRFTAEELTDLYVTEGPRIFDNSLLRTVGSAGGLADERYDSDG
                     LVAALETYVGRTRLSEALVPVLLTAYDIEGRQAFFFRTERARADPEYDFALADAALAT
                     AAAPTYFEPVRVTDVAGAATYPLVDGGVFAANPAMCAWTDLIRAGVAPDVTVLASLGT
                     GTAIRPIPYEQARGWGLLGWARPLIDVLFSGASETVDFQLGQILGDRYMRFQQRLEIA
                     SDDMDDASPENLRDLRAEGDLLVRENTEAIDDLCARLSE"
     gene            1158..2144
                     /gene="trpS"
                     /locus_tag="VFY44_09555"
     CDS             1158..2144
                     /gene="trpS"
                     /locus_tag="VFY44_09555"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX5910727.1"
                     /translation="MRIFSGIQPTGRKHLGNYIGAVRGWVEGQDRGEPAIYCMVDLHA
                     VTVPYEPAELRRYVYDTTALLMASGLDPERCVLFRQSDVHEHTELTWLLSSVTAHGDL
                     NRMTQFKEKSAQQRELVSAGLFLYPMLQAADVLVYRAHEVPVGEDQRQHIELMRDVAM
                     RFNATYGETLVVPEHRIPEVGARIMDLQDPTSKMSTTSSEEKGRIYIDDDEKSVLKKV
                     KSAVTDSGGEVRRGPGKEGITNLIDILAVVRGVGPAEIEAEFEGQQYGAFKGAVAEAV
                     VDYLRPVRERYAELRPDETVLEAALDRGAEQARVLAAETMVDVRAAMGVGKP"
     gene            complement(2141..3001)
                     /locus_tag="VFY44_09560"
     CDS             complement(2141..3001)
                     /locus_tag="VFY44_09560"
                     /inference="COORDINATES: protein motif:HMM:NF004526.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase/reductase"
                     /protein_id="HEX5910728.1"
                     /translation="MASYPVNGKIAMVTGAARGIGFGTAQELTKRGATVVVVDLDQSA
                     ADEAAERLGPDAMGIGADVTDHGAMRAAVAATVERYGRLDIVMANAGIASTAATVNAM
                     SGESAERVLDVNLHGVYRTVTAALPEIIRNKGHVVVVASVYAFANGVGTAPYAMAKAG
                     VEQLGRALRVELAQHGAGASVAYFGFIDTEMVRRGIDQDPLSGKVWENTPKILHKRLQ
                     PSQAGTGVVDGIEKRAPRIIRPRRWAVFSVLRGILNPLLDTAMVRDAGTQEFAAELDK
                     RGDQEQATTA"
     gene            complement(3014..>3474)
                     /locus_tag="VFY44_09565"
     CDS             complement(3014..>3474)
                     /locus_tag="VFY44_09565"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910729.1"
                     /translation="IDFADLSTTPVALELGGWRLAVLDSGERHAHAESGYNERRAECE
                     RGDPRRLSHVAGENDRVREAVLALGAGDMARLGELLDASHASLRDDYEVSTPAVEATR
                     ERLLEAGAAGARIMGGGFGGAVLGLFPPGARPPRDALAVQPGRTAQLLTT"
BASE COUNT          460 a         1270 c         1259 g          485 t
ORIGIN      
        1 ctgacgttgc cgttcgtggg catcctgttc gggctcgccg cggtggcgct gacctaccgc
       61 accccgctgc cgcccgcgcg catcgccggg ctggcgctgg ccgcgctgtg cctcgtcctc
      121 gtcgtgaccg gcgttgggcc gggcgacggc ggcgggttgg tcgactagag cgccgcggcg
      181 cggtagcgtc gccccatgaa ggtgctgtcc atcgacggcg gtggcatccg ggggctgatc
      241 ccggccgtgg tgctggccga gatcgagcgc cgctccgggc gtcggatcgc agacctgttc
      301 gacctgatcg cgggcacctc gacgggcggc atcctggcgg cggccctggc gtgcccggac
      361 ggggacggcc gcccgcgctt cacggccgag gagctgaccg acctgtacgt gacggagggg
      421 ccgaggatct tcgacaactc gctgctgcgc acggtcggct ccgccggcgg gctggccgac
      481 gagcgctacg actccgacgg cctggtggcg gcgctggaga cctacgtcgg ccggacgcgc
      541 ctgtcggagg cgctggtgcc cgtcctgctg acggcctacg acatcgaggg gcggcaggcg
      601 ttcttcttcc gcaccgagcg ggcacgggcc gaccccgagt acgacttcgc gctggccgac
      661 gcggcgctgg ccaccgcggc ggccccgacc tacttcgagc cggtgcgggt caccgacgtc
      721 gccggcgccg ccacctatcc cctggtggac ggcggcgtgt tcgcggccaa cccggccatg
      781 tgcgcctgga ccgacctgat ccgcgccggc gtggcgcccg acgtgaccgt gctggcctcg
      841 ctgggcacgg gcaccgccat ccgcccgatc ccgtacgagc aggcgcgcgg ctggggcctg
      901 ctggggtggg cgcggccgct gatcgacgtg ctgttctcgg gcgcgtccga gaccgtcgac
      961 ttccagctgg gccagatcct gggtgaccgc tacatgcggt tccaacagcg gctggagatc
     1021 gcgtccgacg acatggatga cgcgtcgccg gagaacctgc gcgacctgcg cgcggagggc
     1081 gacctgctgg tgcgcgagaa cacggaggcc atcgacgacc tctgcgcccg cctgtccgaa
     1141 tagcctgcac gccggacatg cggatcttca gcggcatcca gcccaccggg cgcaagcacc
     1201 tgggcaacta catcggggcc gtccgcggct gggtcgaggg ccaggaccgg ggcgagcccg
     1261 ccatctactg catggtcgac ctgcacgccg tcaccgtgcc ctacgagccg gccgagctgc
     1321 gccgctacgt gtacgacacc accgcgctgc tcatggcctc gggcctggac ccggagcgct
     1381 gcgtgctgtt ccgccagtcc gacgtgcacg agcacaccga gctgacctgg ctgctgtcgt
     1441 cggtcaccgc ccacggcgac ctgaaccgga tgacccagtt caaggagaag tccgcccagc
     1501 agcgcgagct ggtgtcggcc gggctgttcc tgtacccgat gctccaggcc gccgacgtgc
     1561 tggtctaccg cgcccacgag gtgccggtcg gcgaggatca gcgccagcac atcgagctga
     1621 tgcgcgacgt ggcgatgcgc ttcaacgcca cctacggcga gacgctggtg gtgcccgagc
     1681 accgcatccc ggaggtcggc gcacgcatca tggacctgca ggacccgacg tcgaagatga
     1741 gcaccaccag ctcggaggag aagggccgga tctacatcga cgacgacgag aagagcgtcc
     1801 tcaagaaggt caagagcgcc gtcaccgact ccggcgggga ggtcaggcgc ggccccggca
     1861 aggaggggat cacgaacctg atcgacatcc tggccgtcgt ccgcggggtg ggcccggcgg
     1921 agatcgaggc cgagttcgag ggccagcagt acggcgcctt caagggcgcg gtggccgagg
     1981 ccgtggtgga ctacctgcgc ccggtgcgcg agcgctacgc cgagctgcgg ccggacgaga
     2041 cggtcctgga ggcggcgctg gaccgcggcg ccgagcaggc ccgtgtgctg gccgccgaga
     2101 cgatggtcga tgtgcgcgcc gcgatgggcg tcggcaagcc ctaggccgtc gtcgcctgct
     2161 cctggtcgcc gcgcttgtcc agctcggcgg cgaactcctg ggtgccggcg tcgcgcacca
     2221 tcgcggtgtc cagcagcggg ttcaggatcc cgcgcagcac cgagaagacc gcccagcggc
     2281 ggggccggat gatgcgcggc gcgcgcttct cgatgccgtc caccacgccc gtgccggcct
     2341 gcgagggctg gaggcgcttg tgcaggatct tcggcgtgtt ctcccacacc ttcccggaga
     2401 gcgggtcctg gtcgatcccg cggcgcacca tctcggtgtc gatgaagccg aagtaggcca
     2461 cgctggcgcc cgcgccgtgc tgggccagct ccacgcgcag cgcgcggccc agctgctcca
     2521 cgcccgcctt ggccatggcg tagggcgcgg tgcccacgcc gttggcgaag gcgtacacgg
     2581 atgcgacgac gaccacgtgg cccttgttgc ggatgatctc gggcagcgcg gcggtcaccg
     2641 tgcggtagac gccgtgcagg ttcacgtcca ggacccgctc ggcggactca ccggacatgg
     2701 cgttcacggt cgcggccgtc gaggcgatcc cggcgttggc catgacgatg tccagccggc
     2761 cgtagcgctc cacggtggcg gccacggcgg cgcgcatcgc gccgtggtcg gtcacgtcgg
     2821 cgccgatgcc catcgcgtcc gggcccagcc gctccgcggc ctcgtcggcc gcgctctggt
     2881 ccaggtccac gaccacgacg gtcgcccccc gcttggtcaa ctcctgcgcg gtgccgaagc
     2941 cgatgccgcg cgcggccccg gtgaccatcg cgatcttgcc gttcactgga tagctcgcca
     3001 tggcgcgagt ctttcaggtg gtgagcagct gcgccgtcct gcctggctgg acagccaggg
     3061 cgtcgcgcgg gggccgggcg cccggcggga acagccccag caccgcaccg ccgaacccgc
     3121 cgcccatgat ccgcgcaccg gccgcgcccg cctccagcaa ccgctcacgc gtggcctcga
     3181 ccgcgggagt cgagacctcg tagtcgtcgc gcaggctggc gtgtgaggcg tccagcagct
     3241 cacccagccg cgccatgtca cccgcgccca gcgcgagcac cgcctcgcgc acgcggtcgt
     3301 tctcgccggc cacgtggctc agccggcgcg gatcgccccg ctcgcactcc gcgcggcgct
     3361 cgttgtagcc cgattccgcg tgggcgtggc gctcgccgga gtccagcacc gccagccgcc
     3421 agccgcccag ctccagggcc accggggtgg tggacaggtc ggcgaagtcg atca
//
LOCUS       DASUPW010000424         3475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1343156, whole genome shotgun sequence.
ACCESSION   DASUPW010000424 DASUPW010000000
VERSION     DASUPW010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343156"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VFY44_09570"
     CDS             <1..228
                     /locus_tag="VFY44_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910730.1"
                     /translation="DPAKVIETIDYSHPVFTPEGVAAQGRQMEISGADRIHYCGAYWR
                     WGFHEDGVVSALRALDRVGGAPAPALQAVAA"
     gene            225..977
                     /locus_tag="VFY44_09575"
     CDS             225..977
                     /locus_tag="VFY44_09575"
                     /inference="COORDINATES: protein motif:HMM:NF018770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HEX5910731.1"
                     /translation="MTASAIYDGWVRHRRHEPVDHDFSYRHAMLLLDLDELPRVLDVK
                     PFYSATGRALARFRREDYLGDPDQPLAESVRDLVEQRTGTRPPGPVRLLTTLRTFGHS
                     FNPVSFYYCFARGGERVEAVVAHVTNTPWGESHAYVLSRDGEASVMRDTMDKVFHVSP
                     FIGMDGHYDWRVTEPGRQLLVHIDETGDGGAHVFDATLSLKRRELTPRRLMGLLARFP
                     LTSLRVVGLIYWNALKLKLKGAPYFRHPRKAT"
     gene            977..2212
                     /locus_tag="VFY44_09580"
     CDS             977..2212
                     /locus_tag="VFY44_09580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX5910732.1"
                     /translation="MRDAAARRVVFKLFSLIKRERIDVIDHGRTFSFGDPDAELRATI
                     VVHQPDFYRNMLRGSIGLAETYMDGHWDTDDLVALTRIGARNMAAIDKVRRDWHPVLH
                     RAQKLADMVPRNDIAGSKRNIAAHYDLGNQLFSLFLDPTMMYSCAYFEEPGQSLEDAQ
                     RAKLERACRALELGPDDHLLEIGTGWGAMAIHAAENHGCRVTTTTISREQHAYATEQV
                     RDRGLEDRITVLLEDYRDLTGTYDKLVSIEMIEAVGWQFFPTYFRHCSRLLADDGLMF
                     LQAITIDERSYEVEKQSKSFINTHIFPGGCLPSLEVIHRCVAQETDMTTVWLDDITQH
                     YAETCLRWREAFTGSAELAAEMGYDETFRRMWQLYLAYVEAGFRERRIMDVHMLLSKP
                     EWRQSLPVEPAAEEAAWAR"
     gene            2299..3282
                     /locus_tag="VFY44_09585"
     CDS             2299..3282
                     /locus_tag="VFY44_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910733.1"
                     /translation="MVVALLLAGSVLAAVHHAEVVAHRVGEPYGALVLAVAVTVIEVG
                     LIVTLMVSGDPDEAATLARDTVFAAVMITCNGILGLSVLTGALRRGSAHFNSEGSATA
                     FATVLTLATVCLVLPSFTSSAEGPEFTGSQLAFAAIASLALYGLFVWIQTIRHRDYFL
                     PVESDAGEDAHAEPPSDRATWASLGLLLVSLVSVVGLAKIESPGIEDGISAIGAPASA
                     VGVVIAVLVLSPETLAAVRNARRGRVQTSLNLAFGSAMASIGLTIPVLAVASIWLDGP
                     LELGLGGTQLVLLVITGLVGALTILPGRATVQEGGVHLVLLFAFLFFAIHP"
     gene            3279..>3475
                     /locus_tag="VFY44_09590"
     CDS             3279..>3475
                     /locus_tag="VFY44_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5910734.1"
                     /translation="MTPLAYDRAGSGPALALIHPLGAHRGVWKPVLPLLEDRFDIVAV
                     DMPGFGDSADLPEDVAATPLN"
BASE COUNT          489 a         1283 c         1177 g          526 t
ORIGIN      
        1 gaccccgcga aggtgatcga gaccatcgac tactcccacc ccgtgttcac gcccgagggc
       61 gtggccgcgc agggccgcca gatggagatc tcgggcgccg accggatcca ctactgcggc
      121 gcctactggc gctggggctt ccacgaggac ggcgtggtca gcgccctgcg cgcgctggac
      181 cgcgtcggcg gcgcgccggc gccggcactc caggcggtgg ccgcgtgacc gcctccgcca
      241 tctacgacgg ctgggtgcgc caccgccgcc acgagccggt cgaccacgac ttcagctacc
      301 gccacgcgat gctgctgctg gacctggacg agctgccgcg cgtgctggac gtcaagccgt
      361 tctactcggc caccggccgc gcgctggcgc gcttccggcg cgaggactac ctgggcgacc
      421 ccgatcagcc gctggccgag tccgtgcgcg acctggtcga gcagcggacc ggcacccgcc
      481 cgcccgggcc ggtgcggctg ctgaccacgc tgcgcacctt cggccactcg ttcaacccgg
      541 tgagcttcta ctactgcttc gcccgcggcg gcgagcgcgt ggaggccgtc gtggcccacg
      601 tgaccaacac gccgtggggc gagagccacg cgtacgtgct ctcccgcgac ggagaggcgt
      661 cggtcatgcg cgacacgatg gacaaggtct tccacgtgtc gcccttcatc ggcatggacg
      721 gccactacga ctggcgcgtg accgagcccg gccggcagct gctggtgcac atcgacgaga
      781 ccggcgacgg cggcgcgcac gtgttcgacg ccacgctctc gctcaagcgc cgcgagctga
      841 ccccgcggcg gctgatgggc ctgctggccc gcttccccct gacctcgctg cgcgtggtcg
      901 ggctgatcta ctggaacgcg ctgaagctca agctcaaggg cgccccgtac ttccgccacc
      961 cccggaaggc cacctgatgc gcgacgccgc cgcccgccgg gtcgtgttca agctgttctc
     1021 gctgatcaag cgcgagcgca tcgacgtgat cgaccacggc cgcaccttca gcttcggcga
     1081 tcccgacgcc gagctgcgcg cgacgatcgt cgtgcaccag cccgacttct accgcaacat
     1141 gctgcgcggc tcgatcggcc tggccgagac ctacatggac ggccactggg acaccgacga
     1201 cctggtggcg ctgacccgga tcggcgcccg caacatggcc gcgatcgaca aggtccggcg
     1261 cgactggcac cccgtgctgc accgcgccca gaagctggcc gacatggtcc cgcgcaacga
     1321 catcgccggg tcgaagcgca acatcgccgc ccactacgac ctgggcaacc agctgttctc
     1381 gctgttcctg gaccccacga tgatgtactc gtgcgcctac ttcgaggagc cgggccagtc
     1441 gctcgaggac gcgcaacggg ccaagctcga gcgcgcctgc cgcgcgctgg agctggggcc
     1501 ggacgaccac ctgctggaga tcggcaccgg ctggggcgcg atggccatcc acgccgccga
     1561 gaaccacggc tgccgcgtga ccaccacgac gatctcgcgc gagcagcacg cctacgcgac
     1621 cgagcaggtg cgcgaccgcg gcctggagga ccggatcacg gtgctgctgg aggactaccg
     1681 cgacctgacc ggcacctacg acaagctcgt gtcgatcgag atgatcgagg cggtgggctg
     1741 gcagttcttc cccacctact tccggcactg ctcacggctg ctggccgacg acgggctgat
     1801 gttcctgcag gccatcacca tcgacgagcg ctcctacgag gtcgagaagc agtccaagag
     1861 cttcatcaac acccacatct tccccggcgg ctgcctgccc tcgctggagg tcatccaccg
     1921 ctgcgtggcg caggagaccg acatgaccac ggtctggctg gacgacatca cccagcacta
     1981 cgcggagacg tgcctgcgct ggcgcgaggc gttcaccggg agcgccgagc tggcggccga
     2041 gatgggctac gacgagacgt tcaggcgcat gtggcagcta tacctcgcgt atgtcgaagc
     2101 cggcttccgc gagcgccgca tcatggacgt gcacatgctg ctgagcaagc ccgagtggcg
     2161 ccagtcgctg cccgtggagc cggccgctga ggaggcggcc tgggcgcgct gagacattgg
     2221 accgcgggag tcccggtcct gtcgctggtc gcgctcgccg tcttctggcc gcagaaggac
     2281 ccgagcaccg cggtgctcat ggtcgtggcc ctgctcctgg ccggctcggt gctggccgcg
     2341 gtccatcacg ccgaagtggt ggcccacagg gtgggcgagc cctatggggc gctcgtcctg
     2401 gccgtggcgg tcacggtcat cgaggtcggg ctgatcgtga ccctgatggt gtccggcgat
     2461 cccgacgaag ccgcgacgct ggcccgcgac acggtcttcg cagcggtgat gatcacctgc
     2521 aacgggatcc tcgggctgtc ggtgcttacg ggagcgctgc ggcgcggctc ggcccacttc
     2581 aactccgagg gctcggccac cgccttcgcc acggtcctga cgctggccac ggtatgcctg
     2641 gtgctgccct cgttcaccag cagcgccgag ggacccgagt tcacgggctc ccagctggcc
     2701 ttcgccgcca tcgcctcgct ggccctgtac ggcctgttcg tctggatcca gacgatccgc
     2761 caccgcgact acttcctgcc cgtggagagc gacgcgggcg aggacgccca cgccgagcct
     2821 cccagcgacc gtgccacctg ggccagcctc ggcctgctgc tggtgtcgct ggtgtcggtg
     2881 gtcgggctgg cgaagatcga gtcgcccggc atcgaggacg gcatctccgc catcggcgca
     2941 cccgcgtcgg ccgtcggcgt ggtgatcgcg gtgctggtgc tctcgcccga gacgctcgcc
     3001 gccgtgcgca acgcgcgccg cggccgggtg cagaccagcc tgaacctggc cttcggctcg
     3061 gccatggcca gcatcgggct gaccatcccg gtcctggccg tcgcgtcgat ctggctggat
     3121 gggccactgg agctgggcct cggcggcacc cagctcgtgc tgctggtgat caccgggctc
     3181 gtgggcgcgc tgacgatcct gcccggccgc gccaccgtcc aggagggcgg ggtgcacctg
     3241 gtcctcctgt tcgcgttcct gttcttcgcg atccatccat gacgccgctc gcgtacgacc
     3301 gggccggctc cgggccggcg ctggcgctga tccaccccct cggcgcgcac cgcggcgtgt
     3361 ggaagcccgt cctgccgctc ctcgaggacc gcttcgacat cgtggccgtg gacatgcccg
     3421 gcttcggcga ctcggccgac ctgcccgagg acgtggccgc cacaccgctg aacat
//
LOCUS       DASUPW010000425         4354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_790014, whole genome shotgun sequence.
ACCESSION   DASUPW010000425 DASUPW010000000
VERSION     DASUPW010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790014"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VFY44_09595"
     CDS             <1..331
                     /locus_tag="VFY44_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5910735.1"
                     /translation="GSTFAGGPVIAAGANAVLDVISEDGFLAAVAAKGARLRSGLEGL
                     GLEVRGRGLMLAFATADAPDTARRLLLEHRLVVNATGPDTIRLLPPLTVSDEEIDEAV
                     ARISRSS"
     gene            complement(315..1151)
                     /locus_tag="VFY44_09600"
     CDS             complement(315..1151)
                     /locus_tag="VFY44_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910736.1"
                     /translation="MIALLPCLAPASAAADSRVTGVIGGPATVKRGQEAIYTVTLRNA
                     GSQTLGSGSPRLAMDLDSIPSSGASLTGAVQIGGTGPWSCGSSGCTLRSGVTFPPGAT
                     ASFAVAAKVFRSTTLDLDVTGNEVSFSNVRRSVRTSGTLVPVLYGLAIPATASPRTGP
                     IGFTLSEPSRVTVRLERISTRWRLRKRVGGRVVRSIRCSDRKPRVRRTTLVRKGRTRC
                     SPGLRTLFTSPTRPRGFNLLDVPQLRTLTVGRRYRLRLIPRTPDGRQGREARSLVFKT
                     TG"
     gene            1234..2505
                     /locus_tag="VFY44_09605"
     CDS             1234..2505
                     /locus_tag="VFY44_09605"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX5910737.1"
                     /translation="MAEDRDNQKLGSYEAAPGDVGRVLLLYSGGLDTSVMLKWIQDHY
                     GAEVVALTVNLGQPGEDFEVVKGKALKLGALSCEVVDAREEFAEEYVLPAIKANARYG
                     GGYPLFTALGRPLIAKIAVDKAREHGCDAVAHGCTGKGNDQVRIDGTVLTLAPEMKIV
                     APVRNWAMGREEEIAYAREHGIPVKGGTEAPPYSIDDNIWGRSSEGGPIEDLSEPPRD
                     DVFELVADARDAPDEAQDLTIGFEQGRPVSLDGDRIGLVDLIDRVAAAGRTHGVGILD
                     HIEDRIVGLKVRDLYEVPAAEILLTAHAELEKLVCTIHQNEFKFMLDRKWAYLAYAGL
                     WHEPLRGDLDAYMDSVNAQVTGEIKVRLYKGKASVVARSSPHALYDQELAGFGESGGL
                     FSQEASPGFIELWSLQSRMAYAVRNREARGK"
     gene            2502..2837
                     /locus_tag="VFY44_09610"
     CDS             2502..2837
                     /locus_tag="VFY44_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910738.1"
                     /translation="MRRIGLILCFAVLACAAPAPVAAAPPQATTARACGSVKLTLGKS
                     IVRAKNVRCADARRFVAAVLARDCGETRDCNFSRFTFRGYTCRKSVSAKLTKNSCTKG
                     RKAISELHA"
     gene            2851..3021
                     /locus_tag="VFY44_09615"
     CDS             2851..3021
                     /locus_tag="VFY44_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910739.1"
                     /translation="MTNKSSYTVLGWIVWQIGSRLAKRKATQNKGKLGAGAAVALIVI
                     GGILVAAGDSDD"
     gene            complement(3018..>4354)
                     /locus_tag="VFY44_09620"
     CDS             complement(3018..>4354)
                     /locus_tag="VFY44_09620"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX5910740.1"
                     /translation="RAAASDLTNEKGTVTRFKREVAGLDTLATAAAKRAGGSPLAPGL
                     FGEGGAWIDYRGPAGTIPTVSFSDVLRGRFPAAAVRDRVVVVGAAAPTLRDVHPTPAG
                     DELMAGAEVQANAIWTAMHGVPLRSAPAWLSLALIVLLALVAPLAGWRLPGVVAVPVL
                     LLTALAFLVGAQLAFGAGLMVPVVAPLVALLTATVAFITWSRLSERRARLDTLRDNAI
                     LEQRVRERTDELWQTQLEVVRRLGRAVDWRDGDTGEHVDRIGRFCERLGSASGMGPSE
                     AELLRHASVLHDVGKVGIPDGILNKPGKLDAREWEIMKTHTTIGGDILEGAESELMDL
                     ARSIALTHHENWDGSGYPAGLRGEEIPLAGRICAICDVFDALLSPRPYKEPWALEDVL
                     TELASLRGTKFDPELLDAFLAIAPSLHAESFAPSAGAYVVAGDQQAGVPAGA"
BASE COUNT          641 a         1541 c         1619 g          553 t
ORIGIN      
        1 cggctcgacg ttcgccggcg ggccggtgat cgcggccggc gccaacgccg tgctggacgt
       61 gatctccgag gacggcttcc tggcggcggt cgcggcgaag ggcgcccggc tgcggagcgg
      121 gctggagggc ctggggctgg aggtgcgcgg ccgcggcctg atgctcgcct tcgccaccgc
      181 cgacgccccg gacaccgccc ggcgcctgct gctcgagcac cggctcgtgg tcaacgccac
      241 cggaccggac acgatccgcc tgctgccgcc gctgacggtc tccgacgagg agatcgacga
      301 ggccgtggcc cggatcagcc ggtcgtcttg aacacgagcg acctggcctc gcggccctgg
      361 cgcccgtccg gcgtccgcgg gatcaggcgc agccggtaac ggcgccccac ggtcagggtg
      421 cgcagctggg gcacgtcgag caggttgaac ccgcggggcc gcgtggggct ggtgaacagg
      481 gtccgcaggc ccgggctgca gcgcgtgcgg cccttgcgca cgagcgtggt gcgccgcacc
      541 cggggcttgc ggtccgagca ccggatcgag cgcaccacgc ggccgcccac gcgcttgcgg
      601 agccgccagc gggtggagat gcgctccagg cggacggtca cccgagaggg ctcggacagc
      661 gtgaagccga tcggcccggt ccgcggcgag gccgtggcgg ggatcgccag gccgtagagc
      721 acgggcacca gcgtcccgct ggtgcgcacc gacctgcgca cgttcgagaa cgacacctcg
      781 ttgccggtca cgtccaggtc cagcgtcgtg gagcggaaca ccttggccgc gacggcgaac
      841 gaggcggtcg cgcccggtgg gaacgtgacc ccgctgcgca gggtgcaccc cgacgagccg
      901 caggaccacg ggcccgtgcc accgatctgg accgcgcccg tgaggctcgc gcccgaggag
      961 gggatcgagt ccaggtccat cgccaggcga gggctgccgc tgcccagcgt ctggctgccg
     1021 gcgttgcgga gggtcacggt gtagatcgcc tcctggccgc gcttgacggt cgcgggcccg
     1081 ccgatcaccc ccgtgacgcg cgagtccgcg gccgcggagg ccggtgccag gcagggcagg
     1141 agggcgatca tggaaaccag gaggtatcgg cgcatacgga ctgtgtaacc cgcctcgggc
     1201 tcgaatggtg cggctggtag cttgccccgt tcgatggctg aagaccgcga caaccagaag
     1261 ctcggcagct acgaggccgc ccccggcgat gtcggccggg tgctcctcct gtactcgggc
     1321 ggcctggaca ccagcgtcat gctcaaatgg atccaggacc actacggcgc cgaggtggtg
     1381 gccctgaccg tgaacctggg ccagcccggc gaggacttcg aggtggtcaa gggcaaggcg
     1441 ctcaagctgg gcgcgctgtc gtgcgaggtc gtggacgcgc gcgaggagtt cgccgaggag
     1501 tacgtgctcc cggcgatcaa ggccaacgcc cgctacggcg gcggctaccc gctgttcacc
     1561 gccctgggcc gcccgttgat cgccaagatc gcggtcgaca aggcgcgcga gcacgggtgc
     1621 gatgcggtcg cacacggctg caccggcaag ggcaacgacc aggtacgcat cgacggcacc
     1681 gtgctgacgc tggcgccgga gatgaagatc gtcgccccgg tgcgcaactg ggccatgggc
     1741 cgcgaggagg agatcgccta cgcccgtgag cacggcatcc cggtcaaggg cgggaccgag
     1801 gcgccgccct attcgatcga cgacaacatc tggggccggt cgtcggaggg cgggccgatc
     1861 gaggacctct ccgagcctcc gcgcgacgac gtgttcgagc tggtggccga cgcgcgcgac
     1921 gccccggacg aggcccagga cctgaccatc ggcttcgagc aggggcggcc ggtgtccctg
     1981 gacggcgacc ggatcggcct cgtggacctg atcgaccgcg tcgccgccgc cgggcgcacc
     2041 cacggggtcg gcatcctgga ccacatcgag gaccggatcg tcggcctgaa ggtgcgcgac
     2101 ctgtacgagg tcccggccgc cgagatcctg ctgaccgcgc acgccgagct ggagaagctg
     2161 gtgtgcacga tccaccagaa cgagttcaag ttcatgctcg accgcaagtg ggcctacctg
     2221 gcctacgcgg ggctgtggca cgagccgctg cgcggcgacc tggacgccta catggactcc
     2281 gtgaacgctc aggtcaccgg cgagatcaag gtccgcctgt acaagggcaa ggcgtcggtg
     2341 gtggcacgga gctcgccgca cgccctgtac gaccaggagc tggccggctt cggcgagtcc
     2401 ggcggcctgt tcagccagga ggccagcccc ggcttcatcg agctgtggag cctccagtcg
     2461 cgcatggctt acgcggtccg caaccgggaa gcacggggca agtgagacgc atcggcctga
     2521 tcctgtgctt cgccgtgctg gcctgtgccg cgcccgcgcc cgtggctgct gcgccgccgc
     2581 aggccacgac ggcccgcgcc tgcggcagcg tgaagctgac gctgggcaag tcgatcgtgc
     2641 gcgccaagaa cgtccgctgc gccgacgccc ggaggttcgt cgcggccgtg ctcgcgcgcg
     2701 actgcggtga gacccgcgac tgcaacttca gccggttcac gttccgcggg tacacctgca
     2761 ggaagagcgt gtccgccaag ctgaccaaga actcgtgcac caagggccgc aaggccatct
     2821 ccgagctgca cgcctgacga aaggacgagg atgaccaaca agagcagcta caccgtgctg
     2881 ggctggatcg tctggcagat cggatcccgc ctggccaagc gcaaggccac gcagaacaag
     2941 ggcaagcttg gcgccggcgc cgccgtggcc ctgatcgtga tcggcggcat cctggtggcc
     3001 gcgggcgaca gcgacgacta ggcgccggcg ggaacgccgg cctgctggtc gccggcgacc
     3061 acgtaggcgc ccgccgaggg ggcgaagctc tcggcgtgca gtgagggcgc gatggccagg
     3121 aacgcgtcca ggagctcggg gtcgaacttg gtcccgcgca ggctcgccag ctcggtgagc
     3181 acgtcctcca gcgcccacgg ctccttgtag ggccgcggcg agagcagggc gtcgaacacg
     3241 tcgcagatgg cgcagatccg gcccgcgagc gggatctcct cgccccgcag cccggcgggg
     3301 tatcccgatc cgtcccagtt ctcgtggtgg gtcagggcga tgctgcgggc caggtccatc
     3361 agctcggact ccgcgccctc caggatgtcg ccgccgatgg tggtgtgtgt cttcatgatc
     3421 tcccactccc gggcgtcgag cttgcccggc ttgttcagga tcccgtcggg gatcccgacc
     3481 ttgcccacgt cgtgcagcac gctggcgtgg cgcagcagct ctgcctcgga ggggcccatc
     3541 ccggaggccg accccagccg ctcgcagaag cggccgatgc ggtccacgtg ctcgccggtg
     3601 tcgccgtcgc gccagtccac cgcgcggccc agacggcgga cgacctccag ctgggtctgc
     3661 cacagctcgt cggtgcgctc gcgcacccgc tgctccagga tcgcgttgtc gcggagggtg
     3721 tccaggcggg cgcggcgctc ggacagccgg ctccaggtga tgaaggccac ggtcgcggtc
     3781 agcagggcca cgaggggggc gaccaccggc accatgagcc cggcgccgaa cgccagctgg
     3841 gcgccgacca ggaaggccag ggcggtcagc agcaggaccg ggaccgcgac cacacccggc
     3901 agtcgccacc cggccagcgg cgccaccagc gccagcagca cgatcagcgc cagcgacaac
     3961 cacgccggcg cgctgcgcag cggcaccccg tgcatcgcgg tccagatggc gttggcctgc
     4021 acctcggctc cggccatcag ctcgtcgccg gcgggcgtgg ggtggacgtc gcgcagggtg
     4081 ggcgccgccg cccccaccac gaccacgcgg tcgcggaccg cggcggcagg gaagcggccc
     4141 cgcagcacgt cggagaagga gacggtcggg atcgtgccgg cgggcccgcg gtagtcgatc
     4201 cacgcgccgc cctcgccgaa cagcccgggc gccagcgggg agccgccggc gcgcttggcg
     4261 gcggcggtcg cgagcgtgtc cagcccggcc acctcgcgct tgaagcgggt caccgtgccc
     4321 ttctcgttgg tcaggtccga ggcggccgct cggg
//
LOCUS       DASUPW010000426         2985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1760782, whole genome shotgun sequence.
ACCESSION   DASUPW010000426 DASUPW010000000
VERSION     DASUPW010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760782"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VFY44_09625"
     CDS             complement(<1..404)
                     /locus_tag="VFY44_09625"
                     /inference="COORDINATES: protein motif:HMM:NF014827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEX5910741.1"
                     /translation="MATATRPAEAEGREGRREILCQDLSVGLQGVIVIDDTTLGPGLG
                     GVRYKPYASMVHAVTECRRLAAAMTLKNAVAELPFGGAKSVIMADRPVADRAALMRRF
                     GDFVAGAGGDYLPGVDMGTAVTDLALISESGAT"
     gene            540..989
                     /locus_tag="VFY44_09630"
     CDS             540..989
                     /locus_tag="VFY44_09630"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX5910742.1"
                     /translation="MSAHATVDETDRRILDLLRQDGRRTVSDIAKHVNLSPAPVKRRI
                     DRLEQTGVIAGYTVVLDHKKVGPSIEAYTELRFVGDTDVDDILNSATELPEVQEVMAI
                     AGDPDALVHVRVDDVEHLQRVVNKLRRTGTVTGTKTLIVMGRWARPE"
     gene            998..2449
                     /locus_tag="VFY44_09635"
     CDS             998..2449
                     /locus_tag="VFY44_09635"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX5910743.1"
                     /translation="MLVVGAGPVGLVLACELARREVPVRVIDRLERPTDQSRAIVVHA
                     RSLEMLQRVGVVDRFLEAGVRSAAMTMHASGREIARVELDMVDSPYPFSLLLPQTETE
                     AILTARLEELGGHVERGVALESFEQDDERVSARLGSGETVEASYVVGADGGHSAVRAQ
                     MGLKLEGSFQGQTFVIADVEAESGHPSESMHTFFSEEGAFLLFPMRDSRVRIIAETDT
                     AAGEPTLESVQAMADARAGGIRLTEAHWLTTFEIHHAQVPTYRVGRAFLAGDAAHVHS
                     PAGGQGMNTGMQDAFNLGWKLALARERHAAPSLLDSYDDERRPVAAQVIRQTTELTRM
                     AVMRHRGARELRNRALHLATGLAPIRRRLADQVEETAINYHLSAIVADDGHHHRGTVR
                     AGDAAPEAKMEDGSSLHELLASRTGHTILHVGNSPPPFDAVLVPPGGAVADRYRLVDG
                     ATVAIRPDGYLGLVASKDGERALERYKAGVLRD"
     gene            complement(2453..>2985)
                     /locus_tag="VFY44_09640"
     CDS             complement(2453..>2985)
                     /locus_tag="VFY44_09640"
                     /inference="COORDINATES: protein motif:HMM:NF037182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX5910744.1"
                     /translation="FPDVTLGVVLDADATLRFPDFRAEERTFALVAQLAGRSGRGSDR
                     GRVVVQALDPEARALQYAARHDSEGFLRGELGRRKALAYPPYGHLVRVVCSSAEPGPE
                     HEAATALRDRLDPPGATVLGPVPLFRRQGKDRAQLVVRGPDREGQIAAVRTAVEEIAA
                     SFSRQGVAVAVDVDPQ"
BASE COUNT          420 a         1098 c         1084 g          383 t
ORIGIN      
        1 gtggcccccg actcgctgat cagggccagg tcggtcacgg cggtccccat gtccacgccg
       61 ggcaggtagt cgccgccggc gccggccacg aagtcgccga agcgccgcat cagggcggcc
      121 cggtcggcca cggggcggtc ggccatgatc accgacttgg cgccgccgaa gggcagctcg
      181 gccaccgcgt tcttgagcgt catcgccgcg gccagccggc ggcactcggt gacggcgtgg
      241 accatgctgg cgtacggctt gtaacggaca cccccaaggc ccggcccgag cgtggtgtcg
      301 tcgatcacga tcacgccctg gagccccacc gacaggtcct ggcagaggat ctcgcgccgt
      361 ccctcacgcc cttccgcttc cgcgggccgc gtagccgtgg ccaccgtgca tctcctccaa
      421 gttcgggttc cctccgcgtg gtgtagcgcc attgatcaca agcgcaaccc acgcgcacgt
      481 ttcgcgcccc cgtgagcaac aaatttccgt tctggccgca tccggcatat ggttgcggtg
      541 tgtcggcgca cgcgacagtc gatgaaaccg accgccggat cctggacctc ctgcgccagg
      601 acgggcggcg caccgtcagc gacatcgcca agcacgtgaa cctgtccccc gcgcccgtca
      661 agcggcggat cgaccggctg gagcagacgg gcgtgatcgc cggctacacc gtcgtcctgg
      721 accacaagaa ggtcggcccc tcgatcgagg cgtacaccga gctgcggttc gtgggcgaca
      781 ccgacgtgga cgacatcctg aactcggcca ccgagctgcc cgaggtgcag gaggtcatgg
      841 ccatcgcagg cgatcccgac gcgctggtgc acgtgcgcgt ggacgacgtc gagcacctgc
      901 agcgggtggt caacaagctg cgccgcacgg gcacggtgac cgggacgaag acgctgatcg
      961 tgatggggcg ttgggcgcgc ccggagtaga ccatccggtg ctggtggtgg gtgccgggcc
     1021 ggtgggcctg gtcctggcct gcgagctggc gcgccgggag gtgcccgtgc gggtgatcga
     1081 ccggctcgag cgccccaccg accagtcgcg cgccatcgtc gtgcacgccc gcagcctgga
     1141 gatgctccag cgggtgggcg tggtcgaccg cttcctcgag gccggcgtgc gcagcgcggc
     1201 gatgacgatg cacgcctccg gccgggagat cgcccgggtg gagctggaca tggtcgacag
     1261 cccctatccc ttctcgctgc tcctgcccca gaccgagacc gaggccatcc tgaccgcgcg
     1321 gctggaggag ctgggcgggc acgtggagcg gggcgtcgcc ctggagtcgt tcgagcagga
     1381 cgatgagcgc gtgtcggccc ggctgggcag cggcgagacc gtcgaggcct cgtacgtggt
     1441 gggcgcggac ggcggccaca gcgcggtccg ggcccagatg ggcctgaagc tggaggggtc
     1501 gttccagggc cagacgttcg tgatcgccga cgtcgaggcc gagtccggtc acccgtccga
     1561 gtcgatgcac acgttcttct ccgaggaagg cgcgttcctg ctgttcccca tgcgcgacag
     1621 ccgcgtgcgc atcatcgccg agacggacac cgccgccggc gagcccacgc tggagagcgt
     1681 gcaggccatg gccgacgccc gcgcgggcgg catccggctg accgaggcgc actggctgac
     1741 caccttcgag atccatcacg cccaggtgcc cacctaccgc gtgggccgcg cgttcctggc
     1801 cggcgacgcc gcccacgtgc acagccccgc cgggggtcag ggaatgaaca ccgggatgca
     1861 ggacgcgttc aacctgggct ggaagctggc gctggcccgc gagcgccacg cggcgccgtc
     1921 gctgctggac agctacgacg acgagcgccg gcccgtcgcg gcgcaggtca tccgccagac
     1981 gaccgagctg acgcggatgg cggtcatgcg ccaccggggc gcgcgcgagc tgcgcaaccg
     2041 cgcactgcac ctggccaccg gcctggcccc gatccgccgg cggctggccg accaggtgga
     2101 ggagacggcc atcaactacc acctgagcgc catcgtggcc gacgacgggc accaccaccg
     2161 cggcaccgtg cgcgcgggcg acgccgcccc ggaggccaag atggaggacg gcagctcgct
     2221 gcacgagctg ctggcctccc ggaccggcca cacgatcctg cacgtgggca actcgccgcc
     2281 gcccttcgac gccgtcctgg tccctcccgg tggggcagtg gcggaccgct accggctggt
     2341 cgacggcgcc accgtggcca tccgccccga cggctacctg ggcctggtgg cctccaagga
     2401 cggcgagcgg gcgctggagc gctacaaggc gggcgtgctg cgggactgac ccttactgcg
     2461 ggtcgacgtc caccgccacc gccacgccct ggcggctgaa cgacgcggcg atctcctcca
     2521 cggccgtgcg caccgcggcg atctggcctt cgcggtccgg gccgcgcacc accagctggg
     2581 cgcggtcctt gccctggcgc cggaacagcg gcaccgggcc cagcacggtg gccccgggcg
     2641 ggtccagtcg gtcgcgcagc gcggtggcgg cctcgtgctc ggggcccggc tcggcgctgg
     2701 agcagaccac gcgcaccagg tgcccgtacg gcgggtaggc cagcgccttg cgccgcccca
     2761 gctcgccgcg caggaagccc tcggagtcgt gccgggcggc gtactgcagc gcgcgggcct
     2821 cggggtccag cgcctgcacc accacccgtc cgcggtccga gccgcggccg ctgcggcccg
     2881 ccagctgggc caccagcgcg aacgtgcgct cctcggcacg gaagtcgggg aagcgcagcg
     2941 tcgcgtcggc gtcgaggacg accccgagcg tcacgtcggg gaagt
//
LOCUS       DASUPW010000427         5320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_652138, whole genome shotgun sequence.
ACCESSION   DASUPW010000427 DASUPW010000000
VERSION     DASUPW010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652138"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1256
                     /locus_tag="VFY44_09645"
     CDS             99..1256
                     /locus_tag="VFY44_09645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5910745.1"
                     /translation="MRILVVPKWYPWPELPVFGVFCREHAAALATRHDVVVLATLFTP
                     DPPDFRVFKLTDQVEDGLRVLRVRYRRPRLRPLALVCQLAGMAAAVRLLRREGWRPGV
                     VHAHVYSAALPALAVGRLSRALVAVTEHYTGFGRGLITGNERRLARFAFERADVVAPV
                     SRELEGTLRELAPKANYVVVPNTVDTDAFEPPAERPPGLRLLNVAALAEKKGHRFLLE
                     AMTELPEATLVIAGDGELRGELEERVRELALEDRVTFSGEQPKHEVARLMREAGLFVL
                     PSLAENLPVVLIEAMASGLPAVATRVGGVPEMLGDEDGVLVEPEDPPALAAAIREAAG
                     RAFDPQAMAARARDRYSYAAVCERWTEVYAGLASSRGSSSEATRRATASSE"
     gene            complement(1184..2209)
                     /locus_tag="VFY44_09650"
     CDS             complement(1184..2209)
                     /locus_tag="VFY44_09650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5910746.1"
                     /translation="MTGSPDIALVSPGTTFGWRYGDAALARHIRQAGAECQVVAVPLG
                     RSAALRRTMATTDLIEALAARRAAGTITAGAVIYSTITAALLQPARSRSAVRFDGLAA
                     VNRPGPGGAWQRRRERTVLERADLLLPWSEQAAPAGHESLVIGPPIATAAPAADAPTV
                     LAYAANPDKRGLDVVCAAWERARPAGGVLGVGGLDRDDGRRWLDKLGGTEPEGVQWLG
                     AVERPRWLSLVAGAGAFLSAARIEDYGMAQMEALAAGTPLVCAPAPGGYAALPLAAEL
                     APALVAPERTGGALGGALSAALALDGPARDDYARRARRLLEPYSDEAVARRVASELLP
                     RLLASPA"
     gene            2239..3330
                     /locus_tag="VFY44_09655"
     CDS             2239..3330
                     /locus_tag="VFY44_09655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5910747.1"
                     /translation="MAGDSRPVALITGTISPYRREPFRLLGQDVALEVLAYEDTGPPI
                     EGLVTRGVTQAEAARLVAGGRYRAVVAGLGGRVALPGAYAAARLRGIPFVLWATIWEH
                     PRTLAHRLSWLPTRHLYRHADAVATYGAHVSRYVERHRGDRGRVFVAPQAVDVDHFAA
                     PVAPEDREAARGRAGAGPGQALFLFVGRLEAEKGIEVLLEAWRTAAPAGARLAFAGTG
                     PLADKIARQGPTVRSLGYVAASQLPALYAAADALVLPSVATATFREPWGLVVNEAMLQ
                     HTPVIASDAVGAAAAGLVDDGRTGLVFPAGDAGALGERIGRLTTDVQAREEMAVRGRT
                     AALALDPAGWARGMRSALDSVLTARGATC"
     gene            3432..4064
                     /locus_tag="VFY44_09660"
     CDS             3432..4064
                     /locus_tag="VFY44_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910748.1"
                     /translation="MRNLIAPALLVLAFSAPAAAQASPDAVVRDCAEDGSVDGSYSNA
                     DKRAALKKIPADLDEYSDCRSAISGSIGSGPKAGAADKKGDSGGGATAASSGGGDGGT
                     PTIDADTNGDGKVTDAEKAAAGKTDLALKQDAKRKNTEASLGDRKTDPTKVGAIDASQ
                     TSNGVTAPVIASIAALALLAAVAALFFLGRRKPGFAQAMRRVPVPGFLRR"
     gene            4153..>5320
                     /locus_tag="VFY44_09665"
     CDS             4153..>5320
                     /locus_tag="VFY44_09665"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX5910749.1"
                     /translation="MILAAGVLLAVALQSFDTRRPLYGGTAVGLFTLLTIVTGLSMAW
                     SVAPDLSLQELGRTFTYLAVFAVAVVAARTLPSAGRHLLTGILLAAVAICLWALATRI
                     WPASLGGDVLGARLGEPFDYWNALGGMAALAVPGALWLGARRDATRVGAALAYPALGA
                     LLLTIMLTQSRGALAAAVVAALVWLAFVPLRLRTAPVLAVAAVGVAPVTAWALSQEAF
                     TAALQPLAVREDAAGTFGLLTVATLAVLTGAGFAAVHIRATMRPSPEVRRRSGIGLAA
                     GLAVVAVVGLVFVGAAGGGIGKQLDELTANQTTAPGSGAERLGSVSSSRGEYWRQAMD
                     VFRDEPLLGRGADGFTLARLEYREDPRAASHAHGYLPQTMADLGLLGLLVALALL"
BASE COUNT          606 a         2028 c         2004 g          682 t
ORIGIN      
        1 gcgatcgagg aggcgtcggc gttcctggcc gaccggccct acgtgcgcac cgagaaggcc
       61 cagcggaccg tgtccaccat ccgccggctg gccccctgat gcggatcctg gtcgtgccca
      121 agtggtaccc gtggcccgag ctgcccgtgt tcggcgtgtt ctgccgcgag cacgccgcgg
      181 cgctggccac ccgccacgac gtcgtggtgc tggccaccct gttcacgccc gaccccccgg
      241 acttccgcgt gttcaagctc accgaccagg tcgaggacgg cctgcgcgtg ctgcgggtgc
      301 gctaccgccg gccccgcctg cgcccgctgg cgctggtgtg ccagctggcc ggcatggccg
      361 cggccgtgcg cctgctgcgg cgcgagggct ggcgccccgg cgtggtgcac gcgcacgtgt
      421 actccgcggc cctgcccgcg ctggccgtgg gccgcctgtc gcgcgcgctg gtggccgtga
      481 ccgagcacta caccggcttc gggcgcggcc tgatcaccgg caacgagcgc cggctggcac
      541 ggttcgcctt cgagcgcgcc gacgtggtgg ccccggtcag ccgcgagctc gagggcacgc
      601 tgcgcgagct cgcgccgaag gcgaactacg tggtggtgcc caacacggtc gacaccgatg
      661 cgttcgagcc gccggccgag cgtcccccgg gcctgcgtct gctcaacgtg gccgcgctgg
      721 ccgagaagaa gggccaccgg ttcctgctgg aggccatgac ggagctgccg gaggcgacgc
      781 tggtgatcgc cggcgacggc gagctgcgcg gcgagctgga ggagcgcgtg cgcgagctgg
      841 cgctggagga ccgggtcacg ttcagcggcg agcagcccaa gcacgaggtc gcgcggctga
      901 tgcgcgaggc cggcctgttc gtgctgccca gcctggccga gaacctgccg gtggtgctca
      961 tcgaggccat ggccagcggc ctgcccgcgg tggccacccg cgtgggcggc gtcccggaga
     1021 tgctcggcga cgaggacggc gtgttggtcg agccggagga cccccccgcc ctggcggccg
     1081 cgatccggga ggcggccggg cgcgcgttcg acccgcaggc catggccgcc cgcgcccgcg
     1141 accgctactc gtacgccgcc gtgtgcgagc gctggaccga ggtctacgcc gggctggcca
     1201 gcagccgtgg cagcagctcg gaggccaccc gccgggcgac cgcctcgtcc gagtagggct
     1261 ccaggagccg gcgcgcgcgc cgcgcgtagt cgtcgcgggc ggggccgtcc agcgccagcg
     1321 cggcggacag ggcgccgccc agcgcgcctc ccgtgcgctc gggcgcgacc agggcgggcg
     1381 ccagctcggc cgcgagggga agcgcggcat agccgcccgg cgcgggcgcg cagaccagcg
     1441 gcgtgcccgc cgccagcgcc tccatctggg ccatgccgta gtcctcgatc cgcgccgcgc
     1501 tgaggaacgc gcccgccccc gcgaccaggg agagccagcg cgggcgctcg accgcgccca
     1561 gccactgcac gccctcgggc tcggtgccgc ccagcttgtc cagccagcgg cgcccgtcgt
     1621 cgcggtccag gcccccgacg cccagcacgc cgcccgccgg ccgcgcgcgc tcccacgccg
     1681 cgcacaccac gtcgaggccg cgcttgtccg ggttggccgc gtaggccagg accgtcggcg
     1741 cgtccgcggc cggcgcggcg gtggcgatgg gcgggccgat caccagcgac tcgtgtccgg
     1801 cgggcgcggc ctgctcgctc cagggcagca gcaggtcggc gcgctccagc accgtccgct
     1861 cgcgccgccg ctgccacgcg ccgcccggac cgggccggtt cacggccgcg agaccgtcga
     1921 agcggacggc cgagcgcgag cgcgcgggct gcagcagggc ggcggtgatg gtcgagtaga
     1981 tcaccgcgcc cgcggtgatc gtcccggccg cgcgccgggc cgccagcgcc tcgatcaggt
     2041 cggtggtggc catggtgcgc cgcagggcgg ccgagcgccc cagcggcacc gccacgacct
     2101 ggcactccgc ccccgcctgg cggatgtgcc gggccagggc ggcgtccccg tagcgccacc
     2161 cgaacgtcgt gcccggcgag accagcgcga tgtcgggcga tccggtcacg ggtggcgcac
     2221 agcgtagggt cacgctggat ggcaggcgac tccaggccgg tcgccctgat cacgggcacc
     2281 atctcgccct accggcgcga gccgttccgg ctgctcggcc aggacgttgc gctggaggtc
     2341 ctggcctacg aggacaccgg gccgccgatc gagggcctgg tcacccgcgg cgtgacgcag
     2401 gccgaggcgg cgcggctggt ggccggcggc cgctaccggg cggtggtcgc cgggctcggc
     2461 ggccgggtcg cgttgcccgg ggcctacgcg gccgcgcggc tgcgcgggat cccgttcgtg
     2521 ctgtgggcga ccatctggga gcacccccgc acgctcgccc accggctgtc ctggctcccg
     2581 acgcgccacc tgtaccggca cgccgacgcg gtcgccacct acggcgcgca cgtcagccgc
     2641 tacgtcgagc gccaccgcgg cgaccgcggg agggtgttcg tggctcccca ggcggtcgac
     2701 gtggaccact tcgcggcccc cgtggcgccc gaggaccgcg aggccgcccg tggacgcgct
     2761 ggagcgggcc ccggccaggc cctgttcctg ttcgtcggcc ggctggaggc ggagaagggc
     2821 atcgaggtgc tgctggaggc ctggcgcacg gccgcgccgg ccggtgcgcg actggcgttc
     2881 gccggcaccg ggccgctggc ggacaagatc gcaagacagg gacccaccgt ccggtccctc
     2941 ggctacgttg ccgcgtccca gctaccagcc ctctacgccg ccgccgatgc gctggtgctg
     3001 ccttcggtgg cgaccgcaac tttccgagag ccatgggggt tggttgtgaa cgaggccatg
     3061 ctccagcaca cacccgtcat agccagcgac gccgtcggcg ccgcagccgc aggcctggtg
     3121 gacgacgggc gcaccgggct ggtgttcccg gcgggtgacg ccggcgccct cggggagcgg
     3181 atcgggcggc tgacaacaga cgtccaggcc cgcgaggaga tggccgttcg gggccgaacc
     3241 gccgctctgg cgctggatcc ggccgggtgg gctcgcggca tgcgatcggc cctcgactcc
     3301 gtcctgacgg ccaggggggc gacttgctag cctctcccgt tcgcagcagg gggaaggtgt
     3361 cgtgcagtcg aagtaagcgc acgtcaccgc tttggcgtac ccgaccttcc gagttaccta
     3421 tgaccagtcg catgagaaat ctcatcgcgc cggcccttct tgtgctggcc ttctccgccc
     3481 cggcggccgc ccaggccagc cctgatgccg tcgtgcgcga ttgcgccgag gacggctcgg
     3541 tggacgggag ctactccaac gccgacaagc gcgcggccct gaagaagatc ccggccgacc
     3601 tggacgagta cagcgattgc cgctcggcca tctccggctc gatcgggtcc ggccccaagg
     3661 ccggcgccgc ggacaagaag ggcgactccg gcggcggcgc cacggccgct tcgagcggcg
     3721 gtggcgacgg cggcaccccc accatcgacg ccgacaccaa cggcgacggc aaggtgaccg
     3781 acgccgagaa ggccgcggcc ggcaagaccg acctggccct caagcaggac gccaagcgca
     3841 agaacaccga ggccagcctc ggcgatcgca agacggaccc cacaaaggtg ggcgccatcg
     3901 acgcgagcca gaccagcaac ggagtcacgg cgccggtgat cgcatcgatc gccgccctgg
     3961 ccctcctggc cgccgtggcc gcgctgttct tcctggggcg ccgcaagccc ggcttcgctc
     4021 aggccatgcg ccgtgtcccg gttccgggct tcctccgccg ctagccctgc ggtcgcgatc
     4081 gccctcgcgg cggtcgcgtt cggagcagcc ggaggtaccc aggtagggcg cgcggcgccc
     4141 acccaggcca tcgtgatcct ggcggccggc gtcctgctgg ccgtcgcgct ccagtccttc
     4201 gacacccggc gcccgctcta cggcgggacc gccgtcggcc tgttcaccct gctgaccatc
     4261 gtcaccgggt tgtcgatggc ctggtcggtg gcccccgacc tgagcctgca ggagctgggt
     4321 cgcacgttca cctacctggc ggtgttcgcg gtcgcggtgg tcgcggcccg cacgctgccc
     4381 tccgccggcc ggcatctgct gacggggatc ctgctggccg cggtggccat ctgcctgtgg
     4441 gcgctggcca cgcgcatctg gcccgcctcc ctgggcggcg acgtgctggg cgcgcgcctg
     4501 ggcgagccgt tcgactactg gaacgcgctc ggtggcatgg ccgcgctggc cgtgcccggg
     4561 gcgctgtggc tgggcgcccg ccgcgacgcc acacgggtcg gggccgcgct ggcctacccg
     4621 gccctgggcg cgctgctgct gacgatcatg ctgacccagt cccgcggcgc gctggccgcc
     4681 gccgtggtgg ccgcgctggt ctggctggcc ttcgtgcccc tgcgcctgcg caccgcgccg
     4741 gtcctggccg tggccgccgt gggcgtggcc ccggtcaccg cctgggcgct gtcccaggag
     4801 gccttcaccg ccgcgctgca accgctggcg gtgcgcgagg acgcggcggg gacgttcggc
     4861 ctgctcacgg tggccaccct cgcggtgctc acgggtgccg gcttcgcggc cgtgcacatc
     4921 cgggccacga tgaggccctc gcccgaggtc cggcgccggt cgggcatcgg cctggccgcg
     4981 gggctggcgg tggtggccgt cgtcgggctc gtgttcgtcg gcgccgcggg cggcgggatc
     5041 ggcaagcagc tggacgagct gacggccaac cagaccaccg cgccgggctc cggcgccgag
     5101 cggctgggct cggtctcgtc gtcgcgcggc gagtactggc gccaggccat ggacgtgttc
     5161 agggacgagc cgctgctggg ccgcggcgcc gacggcttca cgcttgcccg gctggagtac
     5221 cgcgaggatc cccgcgcggc cagccacgcc cacggctacc tcccccagac catggccgac
     5281 ctggggctcc tgggcctgct cgtggcgctg gcgctgctgg
//
LOCUS       DASUPW010000428         2067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1899979, whole genome shotgun sequence.
ACCESSION   DASUPW010000428 DASUPW010000000
VERSION     DASUPW010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1899979"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..695
                     /locus_tag="VFY44_09670"
     CDS             114..695
                     /locus_tag="VFY44_09670"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5910750.1"
                     /translation="MTPLAELSNLADEEVMHLVQEGDPRAFELFYDRHSGPAFSLAYR
                     MVGNRTVAEDVSQEAFLSIWRSKLRYQPDRGSVRTWVLGIVHHRAIDALRRNTVHDKR
                     RASAEGIEERFEARERTEVEVARRDEALTVRAAIDELPAEQSRVIELAYFGGFSHSQI
                     ADQLEMPIGTVKGRMRLGLEKLRKTIAGDPEPA"
     gene            692..1405
                     /locus_tag="VFY44_09675"
     CDS             692..1405
                     /locus_tag="VFY44_09675"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX5910751.1"
                     /translation="MSVERHEMHEQDVGAYVLGALSDEEHTRFVRHLVECPVCRDEVD
                     RLQVAADALPRSVTPLAAPASLKAAVMGQVHADVREREPASLGRRLRERLTRLPAVPA
                     RVASATAVVLLVLGLAAGFGLSQLTTGDDERTVTASFDTDRVVQGSGNLVIAGDDDSG
                     GTLRVHGMPALKRDETYQVWLERDGEVIPKAMFTVSGNGDGLTAVDDLQGADRVMVTK
                     EPAGGARAPSGPPMVTVNL"
     gene            1450..>2067
                     /locus_tag="VFY44_09680"
     CDS             1450..>2067
                     /locus_tag="VFY44_09680"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5910752.1"
                     /translation="MPTCYRHPGRETGVSCSNCGNPICPDCMTSTSVGMRCPDCAGQK
                     TQVRTVRNLTNEPTLTYVLIAVNVIVALGNLLGGGGAANAGGTLVDDGGLSRFHVEQG
                     EYWRLVTSGFLHAGPLHLLFNMFGLYILGGLLEPALGKLQFGILYFVSLLAGAFGALL
                     FEPDALTVGASGAIFGLMGAAVVVMRSRGMNPMESGLGLWIGLNLL"
BASE COUNT          286 a          758 c          733 g          290 t
ORIGIN      
        1 cctggccgcg ctcgagcacg gcctgcgcga ggtctcctcg gacctgcgct cgcgactggg
       61 ccgctgatcc ggctgatccg atcacccacg gggtgcgttc tagcccccag aaggtgactc
      121 ccctggccga actctccaac ctcgccgacg aagaggtcat gcacctggtg caggagggcg
      181 acccccgggc gttcgagctg ttctacgacc gccactccgg ccccgcgttc tcgctggcct
      241 accggatggt gggcaaccgg acggtggccg aggacgtctc ccaggaggcc ttcctgtcca
      301 tctggcgcag caagctgcgc taccagcccg accgcggaag cgtccggacg tgggtgctgg
      361 gcatcgtgca ccaccgcgcc atcgacgcgc tccggcgcaa caccgtgcac gacaagcggc
      421 gcgccagcgc cgaggggatc gaggagcgct tcgaggcccg cgagcgcacc gaggtcgagg
      481 tggcccgccg cgacgaggcc ctgaccgtgc gcgccgccat cgacgagctg cccgccgagc
      541 agtcccgggt gatcgagctg gcctacttcg gcggcttctc ccacagccag atcgccgacc
      601 agctggagat gccgatcggc acggtcaagg gccgcatgcg cctgggcctg gagaagctgc
      661 gcaagaccat cgccggggat ccggagccgg catgagcgtc gagcgccacg agatgcacga
      721 gcaggacgtc ggcgcctacg tgctcggcgc cctgtccgac gaggagcaca cccgcttcgt
      781 ccgccacctg gtcgagtgcc ccgtctgccg ggacgaggtc gaccggctcc aggtcgccgc
      841 ggacgcgctg ccgcgctcgg tgaccccgct ggccgcgccc gcgagcctga aggcggccgt
      901 catgggccag gtgcacgccg acgtgcgcga gcgcgagccc gcctcgctgg gccggcgcct
      961 gcgcgagcgg ctgacccggc tgcccgccgt gcccgccagg gtcgcgtcgg cgaccgccgt
     1021 ggtgctgctc gtgctgggcc tggccgccgg cttcggcctg agccagctca ccaccgggga
     1081 cgacgagcgg accgtgaccg ccagcttcga caccgatcgc gtggtccagg gaagcggcaa
     1141 cctggtgatc gccggtgacg acgactccgg cgggaccctg cgcgtgcacg gcatgcccgc
     1201 cctgaagcgc gacgagacct accaggtgtg gcttgagcgc gacggggagg tcatccccaa
     1261 ggccatgttc accgtcagcg gcaacggcga cggcctgacc gcggtcgacg acctccaggg
     1321 cgccgaccgc gtgatggtca ccaaggagcc cgccggcggg gcacgcgcgc ccagcgggcc
     1381 gcccatggtc accgtgaacc tctgaccctc gcgggccggg gacgcgcgcc aactaccctg
     1441 cgtcgcggaa tgccgacgtg ttaccgccac cccggccggg agaccggcgt gtcctgctcg
     1501 aactgcggga acccgatctg ccccgactgc atgacctcca cctcggtggg catgcgctgt
     1561 cccgactgcg ccggtcagaa gacccaggtg cggacggtgc gcaacctgac gaacgagccg
     1621 acgctgacct acgtgctgat cgcggtcaac gtgatcgtgg cgctgggcaa cctgctgggc
     1681 ggcggcgggg ccgcgaacgc cggcggcacc ctggtggacg acggcggcct ctcgcgcttc
     1741 cacgtcgagc agggcgagta ctggcgcctg gtgacctcgg ggttcctgca cgcgggcccg
     1801 ctgcacctgc tgttcaacat gttcggcctc tacatcctgg gcgggctgct ggagcccgct
     1861 ctgggcaagc tgcagttcgg gatcctctac ttcgtctcgc tgctggccgg cgccttcggg
     1921 gcgctgctgt tcgagcccga tgctctgacc gtgggcgcct cgggcgccat cttcggcctg
     1981 atgggcgccg cggtggtggt catgcgctcc cgcggcatga accccatgga gagcggcctg
     2041 gggctgtgga tcggcctgaa cctgctg
//
LOCUS       DASUPW010000429         2359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1761327, whole genome shotgun sequence.
ACCESSION   DASUPW010000429 DASUPW010000000
VERSION     DASUPW010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1761327"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="VFY44_09685"
     CDS             <1..1146
                     /locus_tag="VFY44_09685"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX5910753.1"
                     /translation="VASMFQVTVVVLIYAVAEGGEFQSYYIFYGSLFFSLAYLSAFRW
                     LFDRVSGYALRAAGYRRRAVIVGKGDQIDAVAHALGGGSDPPLDPVGFVSLTPRPENG
                     MKNLGSLEQLERYFGDIDEVLIADPDFPEDDAVDLVDRCHRSGVRVRVAPSTMEILMD
                     RVEFVPGRAVPLFELKPPVFEGLDFFIKRTFDFFGSIILLIVLSPVLALIALAIKLDT
                     RGPVLFRAPRPGIGGLPFPCLKFRTMQVGAHALQGELEQHNEAGGAIFKIREDPRVTR
                     VGRVLRRFSLDEFPQLWNVFRGDMSLVGPRPLPQRDYDRLEEWHRKRYLVLPGMTGLW
                     QVSGRSELEFDDLVRLDFVYLERWSVFLDLTILLKTIPAVLRSKGAW"
     gene            complement(1163..1831)
                     /locus_tag="VFY44_09690"
     CDS             complement(1163..1831)
                     /locus_tag="VFY44_09690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5910754.1"
                     /translation="MELNDSRILVAGATGTLGGHLTTLLGDAGANLAVAARSGGADTI
                     PLELTDPDSPAAAVAAAAEQLGGLDGLVVATGAVAFGTAGELDDAVTRELFAVNTLGP
                     IALVRAALERIEGPGGVACLSAIVADFPTAGMASYSASKAGLSAYLTAVRREVRKRGI
                     TVLDVRPPHLDTPFSEAALAGEPPKLPEPGDPAELARAVVDALRQDKRILAYDMKQKE
                     LAAS"
     gene            complement(1846..>2359)
                     /locus_tag="VFY44_09695"
     CDS             complement(1846..>2359)
                     /locus_tag="VFY44_09695"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5910755.1"
                     /translation="ELMAPAEADPVLDVGCGSVGLATLGVPGRVTGVDHGDRPDYPGE
                     RFVQADARELPFADGEFAIAYSNSLIEHLPAADRARAAAEMARVGRRLFVQTPNRWFP
                     VEPHALLPLVHWLPRRIGRRLWKLGVTGEPFDDIHLLTARELRGLFPDARIVRERVGP
                     LTKSLIAVRD"
BASE COUNT          293 a          860 c          855 g          351 t
ORIGIN      
        1 gtcgcctcga tgttccaggt cacggtcgtg gtcctgatct acgccgtggc cgagggcggc
       61 gagttccagt cgtactacat cttctacggc agcctcttct tctcgctcgc gtacctgtcg
      121 gcgttccgct ggctgttcga ccgcgtcagc ggctatgccc tgcgggcggc gggctaccgg
      181 cgccgcgcgg tgatcgtcgg caagggcgac cagatcgacg cggtcgcaca cgcgctcggc
      241 ggcggctccg acccgccgct cgaccccgtc ggcttcgtct cgctcacgcc gcggcccgag
      301 aacggcatga agaacctcgg ctcactggaa caactcgagc gctacttcgg cgacatcgac
      361 gaggtgctga tcgccgaccc cgacttcccc gaggacgacg cggtggacct ggtcgaccgc
      421 tgccaccgca gcggcgtgcg ggtgcgcgtc gcgccctcga cgatggagat cctgatggac
      481 cgggtcgagt tcgtgcccgg ccgcgcggtg ccgctgttcg agctcaagcc gccggtcttc
      541 gagggcctgg acttcttcat caagcgcacg ttcgacttct tcggctcgat catcctgctg
      601 atcgtgctct cgcccgtgct ggcgctgatc gccctggcga tcaagctcga cacccgcggg
      661 ccggtgctgt tccgggcgcc gcgccccggg atcggcggcc tgccgttccc gtgcctgaag
      721 ttccgcacga tgcaggtggg cgcgcacgcg ctccagggcg agctcgagca gcacaacgag
      781 gccggcgggg ccatcttcaa gatccgcgag gacccgcggg tcacgcgcgt cggccgcgtg
      841 ctgcgccgct tctcgctgga cgagttcccc cagctgtgga acgtcttccg cggcgacatg
      901 tcgctggtcg ggccgcgtcc gctgccccag cgcgactacg accgcctcga ggagtggcac
      961 cgcaagcgct acctggtgct gccgggcatg accggcctgt ggcaggtctc ggggcgctcc
     1021 gagctggagt tcgacgacct ggtccggctg gacttcgtct acctggagcg gtggtcggtc
     1081 ttcctggacc tgacgatcct gctgaagacg atccccgcgg tgctgcgctc caagggcgcc
     1141 tggtagagcc cgccggccgg tttcacgaag ccgccagctc cttctgcttc atgtcgtagg
     1201 ccaggatgcg cttgtcctgg cgcagcgcgt cgaccacggc ccgcgccagc tcggccgggt
     1261 cgcccggctc cggcagcttc ggcggctcgc ccgccagggc ggcctccgag aacggggtgt
     1321 ccaggtgcgg cgggcggacg tccagcaccg tgatcccgcg cttgcggacc tcgcgccgca
     1381 cggcggtcag gtaggccgac aggccggcct tggacgccga gtacgaggcc atcccggcgg
     1441 tcgggaagtc ggccacgatg gccgacaggc acgccacgcc gcccggcccc tcgatccgct
     1501 ccagcgccgc cctgaccagg gcgatcgggc ccagcgtgtt caccgcgaac agctcgcggg
     1561 tgaccgcgtc gtccagctcg cccgcggtgc cgaaggccac cgcgccggtg gccacaacca
     1621 ggccgtcgag cccgcccagc tgctcggcgg cggccgcgac cgcggcggcg gggctgtcgg
     1681 ggtcggtcag ctcgagcggg atcgtgtccg cgccgccgct gcgggcggcg accgccaggt
     1741 tcgccccggc gtcgcccagc agtgtggtca ggtggccgcc gagggtgccg gtggcgcccg
     1801 ccaccaggat gcgggagtcg ttgagttcca tgtgtggggt ccgtgtcagt cgcgtacggc
     1861 gatcagcgac ttcgtcaacg gccccacgcg ctcgcggacg atccgggcgt ccgggaacag
     1921 cccgcgcagc tcgcgcgcgg tcagcaggtg gatgtcgtcg aagggctcgc cggtcacgcc
     1981 cagcttccac agccggcggc cgatccggcg cggcagccag tgcaccagcg gcagcagcgc
     2041 gtgcggctcg accgggaacc agcggttggg cgtctgcacg aacaggcgcc ggcccacgcg
     2101 cgccatctcg gccgccgccc gggcccggtc ggcggcgggc aggtgctcga tcagcgagtt
     2161 ggagtaggcg atggcgaact cgccgtcggc gaagggcagc tcacgcgcgt cggcctggac
     2221 gaagcgctcg cccgggtagt cggggcggtc gccgtggtcg acgccggtga cccggcccgg
     2281 cacgccgagg gtggccaggc cgacgctgcc acagcccacg tcgagcacgg ggtcggcctc
     2341 ggccggggcc atgagctcg
//
LOCUS       DASUPW010000430         3621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1622598, whole genome shotgun sequence.
ACCESSION   DASUPW010000430 DASUPW010000000
VERSION     DASUPW010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1622598"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VFY44_09700"
     CDS             complement(<1..1026)
                     /locus_tag="VFY44_09700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5910756.1"
                     /translation="MPREPLSIAQVTPYPWEQQHEVNHFVERLAGELRERGHRVVVVA
                     PSDSRELVRDGRARVKAVKAGNQDALFDGDVLAVGQSLPFRRGGSVSLPLDVSRTLED
                     LLENAALDVVHVHEPFAPSAASAALRHSRALNVGTFHSPTERFVSTQVARPLVELLFG
                     RLDGRSASFAATRELVERFFPGGYDVIRPGADLLEPARDGGPPHIVFPVREERGALRI
                     FLRALRRMETDQDWRATVWFPDTAGVSSPPALSRRLRDRVRFAGPADGDEAAHLASAD
                     VVVAASSGTAPAPQLVLRALGAGAVPVASQLPRYAESLDDGELGLMFEPRDALTLAAQ
                     LDRLVGDP"
     gene            1050..1679
                     /locus_tag="VFY44_09705"
     CDS             1050..1679
                     /locus_tag="VFY44_09705"
                     /inference="COORDINATES: protein motif:HMM:NF016240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HEX5910757.1"
                     /translation="MSIEPGAAEIRVLGCLIEKQRTTPDGYPLSVNALRLACNQSTGR
                     DPVVDYDDRTVLDALEQLGRRRWTRLASGAGSRARKYRQLLDRELPASEPEMAVLCVL
                     MLRGPQTPGELKQRTERLHPFADLAAIEDTLLGLIDRELVRGLDRRPGQKERRYEQLL
                     GGAGDEAAAEAPAQAAPAAPVEPDRLGRLEAEVARLRRDLDALKSELGQ"
     gene            1676..2896
                     /locus_tag="VFY44_09710"
     CDS             1676..2896
                     /locus_tag="VFY44_09710"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEX5910758.1"
                     /translation="MTAAHPLRRSDEERKTQSASALELFYDLVFVFAITQVSHHLLEH
                     LTWEGAGQSALMLLMVWWSWNYTTWLTNELNADTVAVRLVVILLMLGSLLMAIAIPEA
                     FGDQALLFAGAYVAIQVGRSAFLAFAGAHAGSMERLRATRILIWFTAAGVFWIAGALA
                     DGSARTILWLIALAIDYGAPAVTYWVPGIARVPLSAWDVSAAHFAERFQLFVIIALGE
                     SIVITGATTAELDLDTGRAAAFAVAFLGSAALWWLYFNYVATIAERRLSLAEDRTAIA
                     RDGYTYLHVLMIAGVIVSAVGDELVIAHPEEHLHGAELAAVAAGPAIYLMAHVLFRLR
                     MTHTVSVPRLTGAAICVALGLVGGQISALALAVLLLAVLVGVILWEQRRAARRRARGE
                     PTPLERLAGSAPPA"
     gene            2930..3595
                     /locus_tag="VFY44_09715"
     CDS             2930..3595
                     /locus_tag="VFY44_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910759.1"
                     /translation="MAALAIGAVTAAPAQAKWYGSTLKGKPNATYGCESALTSGTFGF
                     GLSPTNQSSCTYRHGGYLFRNRFTFIVPGTGRITKIRIKSGPNPPRARLTVLTGSSRV
                     DPFTGRDQPGTYTCCTARYVSKPFKLKANGITTKKTNVKVYDVREKDLNVRIHSSDGL
                     ALSFGSGGRLPLKVTNQLGGFNTGTPQLVGYWPRTAKGDPRVDGYAVSGIDLMFQWNW
                     VRR"
BASE COUNT          504 a         1347 c         1302 g          468 t
ORIGIN      
        1 ggggtcgccc accagtcggt ccagctgggc ggccagcgtc agcgcgtcgc ggggctcgaa
       61 catcaaccca agctcgccgt cgtccagcga ctcggcgtag cgcggcagct gcgaggccac
      121 cgggacggcg ccggcgccca gcgcccgcag caccagctgg ggggcgggcg ccgtgccgct
      181 cgacgcggcc accaccacgt cggccgaggc caggtgggcg gcctcgtccc cgtcggccgg
      241 gccggcgaag cggacgcggt cgcgcagccg ccgcgacagc gcgggcggcg acgacacgcc
      301 ggccgtgtcg gggaaccaga ccgtggcccg ccagtcctgg tcggtctcca tgcgccgcag
      361 cgcgcggagg aagatccgca gcgccccgcg ctcctcgcgg accgggaaca cgatgtgcgg
      421 cgggccgccg tcgcgggcgg gctccagcag gtcggccccg gggcggatca cgtcgtaccc
      481 gccggggaag aagcgctcga ccagctcgcg cgtggcggcg aagctggcgc tgcgcccgtc
      541 caggcggccg aacaacagct ccaccagggg ccgcgcgacc tgggtcgaga cgaaccgctc
      601 ggtgggcgaa tggaacgtgc ccacgttcag cgcgcgcgag tggcgcagcg cggccgaggc
      661 ggcgctgggc gcgaacggct cgtgcacgtg gaccacgtcc agcgccgcgt tctccagcag
      721 gtcctccaac gtccgcgaga cgtccagcgg cagcgacacc gagccgcccc ggcggaaggg
      781 cagcgactgg cccacggcca gcacgtcgcc atcgaacagc gcatcctggt ttcccgcctt
      841 gaccgccttc acccgcgcgc gcccgtcgcg caccagctcg cgcgagtccg acggcgccac
      901 caccaccacg cggtggccgc gctcgcgcag ctcgcccgcc agccgctcca cgaagtggtt
      961 gacctcgtgc tgctgctccc aggggtacgg cgtgacctgg gcgatcgaca ggggttcgcg
     1021 cggcacgcga gcagcatacg attcccgccg tgagcatcga gcccggcgct gccgagatcc
     1081 gcgtgctggg gtgcctgatc gagaagcagc gcaccacgcc cgacggctac cccctcagcg
     1141 tcaacgcgct gcggctggcc tgcaaccagt ccaccgggcg cgacccggtg gtcgactacg
     1201 acgaccggac ggtgctggac gcgctggagc agctggggcg ccggcgctgg acgcggctgg
     1261 ccagcggcgc cggcagcagg gcgcgcaagt accgccagct gctggaccgc gagctgcccg
     1321 cctccgagcc cgagatggcg gtgctgtgcg tgctcatgct gcgcggcccc cagacccccg
     1381 gcgagctcaa gcagcgcacc gagcgcctgc accccttcgc cgacctggcc gcgatcgagg
     1441 acacgctgct gggcctgatc gaccgcgagc tggtgcgcgg gctggatcgc cgcccgggcc
     1501 agaaggagcg gcgctacgag cagctgctgg gcggcgccgg cgacgaggcg gcggccgagg
     1561 ctccggcgca ggccgcgccg gccgcgcccg tcgagcccga tcgcctgggc cgtctcgagg
     1621 ccgaggtcgc caggctgcgc cgcgacctgg acgcgctcaa gtcagagctg ggccagtgac
     1681 cgccgcgcac ccgctgcggc gctccgacga ggagcgcaag acccagagcg ccagcgcgct
     1741 cgagctcttc tacgacctgg tcttcgtgtt cgccatcacc caggtctcgc accatctgct
     1801 cgagcacctg acgtgggagg gcgcggggca gtcggcgctg atgctgttga tggtctggtg
     1861 gtcgtggaac tacacgacct ggttgaccaa cgagctgaac gcggacaccg tcgccgtccg
     1921 gctggtcgtg atcctgctga tgctcgggag cctgctcatg gccatcgcca tccccgaggc
     1981 gttcggcgac caggccctgc tgttcgccgg ggcctacgtg gccatccagg ttgggcgcag
     2041 cgccttcctg gccttcgcgg gcgcgcacgc gggctcgatg gagcggctgc gagccacccg
     2101 gatcctgatc tggttcaccg ccgccggcgt gttctggatc gcgggggcgc tggccgacgg
     2161 atccgcccgc acgatcctct ggctgatcgc gctggccatc gactacggcg cgcccgccgt
     2221 gacctactgg gtcccgggga tcgccagggt gccgctcagc gcctgggacg tcagcgccgc
     2281 ccacttcgcc gagcgcttcc agctgttcgt gatcatcgcc ctcggcgagt cgatcgtgat
     2341 caccggtgcc accacggccg agctggacct ggacaccggc cgcgccgcgg ccttcgcggt
     2401 ggccttcctg ggctcggcgg cgctgtggtg gctgtacttc aactacgtgg ccaccatcgc
     2461 cgagcggcgg ctgtcgctgg ccgaggaccg aaccgcgatc gcgcgcgacg gctacaccta
     2521 cctgcacgtc ctgatgatcg ccggcgtgat cgtgtcggcc gtgggcgacg agctcgtgat
     2581 cgcgcacccc gaggagcacc tccacggcgc cgagctggcg gcggtggcgg ccggcccggc
     2641 catctacctc atggcccacg tgctgttccg gctgcggatg acgcacaccg tcagcgtgcc
     2701 gcgcctgacc ggggccgcga tctgcgtggc gctcggcctg gtgggcggtc agatctccgc
     2761 gctggcgctg gccgtgctgc tgctcgcggt gctcgtcggc gtgatcctgt gggagcagcg
     2821 gcgggcggcg cgcaggcggg cgcgcggtga gcccacgccg ctcgagcgac tggcgggatc
     2881 cgcgccaccg gcataacctg accgggtgcg tcgccggtac cccgttctga tggccgcgct
     2941 cgcgatcggg gcggtcaccg cggccccggc gcaggccaag tggtacggca gcacgctcaa
     3001 gggcaagccc aacgccacgt acggctgcga gtcggcgctg accagcggga ccttcggctt
     3061 cgggctctcg cccaccaacc agtccagctg cacgtaccgc cacggcggct acctgttccg
     3121 caaccggttc acgttcatcg tgccgggcac cgggcggatc acgaagatcc ggatcaagtc
     3181 gggccccaac ccgccccgcg cgcggctcac ggtgctcacg ggctccagcc gcgtcgaccc
     3241 gttcaccggc cgcgaccagc ccgggaccta cacgtgctgc accgcccgct acgtgagcaa
     3301 gcccttcaag ctcaaggcca acggcatcac cacgaagaag acgaacgtca aggtctacga
     3361 cgtgcgcgag aaggacctga acgtccggat ccactcgtcg gacggcctgg cgctgagctt
     3421 cggcagcggt gggcggctgc cgctgaaggt caccaaccag ctcggcgggt tcaacaccgg
     3481 caccccgcag ctggtgggct actggccgcg cacggccaag ggcgacccgc gcgtggacgg
     3541 ctacgccgtc agcgggatcg acctgatgtt ccagtggaac tgggtgcgcc ggtagaacgc
     3601 aaacgggcgg ccccggagga c
//
LOCUS       DASUPW010000431         3018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1345352, whole genome shotgun sequence.
ACCESSION   DASUPW010000431 DASUPW010000000
VERSION     DASUPW010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345352"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..1233
                     /locus_tag="VFY44_09720"
     CDS             145..1233
                     /locus_tag="VFY44_09720"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5910760.1"
                     /translation="MPRTLLTGATGFVGAHLARALTERGDELRVTVRESSDTREIDDL
                     DAEQVTCDVLDRRAVRRAMKGVDRVFHAAGMTSMRPTDAERMFEANVGGARIVLEESL
                     RAQVDGVLFVSSAAALGPAPWGGAADESQLYSGAELRIPYVSSVHEAEGEAMRLAAAG
                     LPLVCVNPAGVFGPGDTHVTSTRLVRSFLLGRVPVYANGAINVVDVRDVARGALLADA
                     RGQVGERYILGGRNFTFDRLFADLGRLSGIEPPVKIPRGVAAAGAGVLGFGPGRTVLA
                     PQEVAAASQYWTYRSGKAKRELGWQARPHEETLADTVAWYLEHEGDRIARSRHSQPMQ
                     YKAAGAFIGAGEGVVENGRRVLRSLRLL"
     gene            1238..1519
                     /locus_tag="VFY44_09725"
     CDS             1238..1519
                     /locus_tag="VFY44_09725"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5910761.1"
                     /translation="MATLYRCKAPTDLVCRCGKVARRLRSAGIDYDEVRVPFLKRDRP
                     EVEELTGQRWVPVLVHGEQVIHESHRILEYLDHVAGQTAPADTRTGAPT"
     gene            1519..1728
                     /locus_tag="VFY44_09730"
     CDS             1519..1728
                     /locus_tag="VFY44_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910762.1"
                     /translation="MAQGAGKGTRLARSRSAAQRAWPVVLMAWERWQSLSDEEKDRYK
                     RQAREYAERGRKAFVQAKRRGPGRH"
     gene            1736..2875
                     /locus_tag="VFY44_09735"
     CDS             1736..2875
                     /locus_tag="VFY44_09735"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5910763.1"
                     /translation="MDSAALADAARAGAGFADDQRRLAPDVAGGLRDSGLLRMLVPAS
                     LGGGEVAPERMVADVEALARADGAAGWCLAVAATGGVLAAFMEPEAAREVFGDARGWV
                     AGVFAPKGRAVATADGDLTVSGRWPFASGSAHSDWLMGGCLVQEGDDVRRSASGAPDV
                     RLALMPTAATTSVDTWSVAGLRGTGSNDFEVDGVVVPQARTVSLVSDTAREPGPLYAF
                     PAFGLLALAIGAVGLGIARGAVDDLVAMASGKVPAMATKTLADQPDTQIRVARAEASL
                     RAARAYVDTAVGEAWELAVQHHDVDLERRAGLRLAASHAMTTAAGVVDEMYSLGGGSS
                     VYESSPLQRRHRDIHVATQHMLVNPSTWEFAGRVLLGAPVRSEQF"
BASE COUNT          362 a         1097 c         1169 g          390 t
ORIGIN      
        1 tcggggggga ggtacgccgt gctgtcggcg acgatggcga cgggcattca ggggaggcta
       61 ccccgtgagg gggtcccgcc ctcactccga tgtcccggcg ccgtgaacgg gtcggctcct
      121 ccgaccctcc atactccgcc ggcgatgccc cgcaccctgc tcaccggcgc caccggcttc
      181 gtcggcgcgc acctggcccg cgccctgacc gagcgcgggg acgagctgcg ggtgaccgtc
      241 cgcgagagct cggacacccg cgagatcgac gacctggacg ccgagcaggt cacgtgcgac
      301 gtgctggacc gccgcgccgt gcggcgcgcg atgaagggcg tcgaccgcgt gttccacgcc
      361 gcgggcatga cgtcgatgcg ccccacggac gccgagcgca tgttcgaggc caacgtgggc
      421 ggggcccgga tcgtgctcga ggagagcctg cgcgcccagg tcgacggcgt cctgttcgtc
      481 tccagcgccg ccgcgctggg cccggcgccc tggggcgggg cggccgacga gtcgcagctg
      541 tactcgggcg cggagctgcg gatcccgtac gtcagctccg tgcacgaggc cgagggcgag
      601 gccatgcgcc tggccgccgc gggcctgccg ctggtgtgcg tcaacccggc gggggtgttc
      661 ggcccgggcg acacccacgt gacctcgacc cggctggtgc gcagcttcct gctcgggcgc
      721 gtgcccgtgt acgcgaacgg cgccatcaac gtggtcgacg tgcgcgacgt ggcccggggc
      781 gccctgctgg ccgacgcgcg cgggcaggtc ggcgagcgct acatcctggg cggtcgcaac
      841 ttcaccttcg accgcctgtt cgcggacctc ggccgcctgt cgggcatcga gcccccggtg
      901 aagatcccgc gcggggtcgc ggcggccggc gccggcgtgc tcggcttcgg tcccggccgc
      961 accgtgctgg ccccgcagga ggtcgcggcg gccagccagt actggaccta ccgctccggc
     1021 aaggccaagc gcgagctggg ctggcaggcg cgcccgcacg aggagacgct ggccgacacg
     1081 gtggcctggt atctcgagca cgagggcgac cggatcgcac gctcgcgcca ctcccagccg
     1141 atgcagtaca aggcggcggg agcgttcatc ggcgccggcg agggcgtggt ggaaaacggg
     1201 cgcagagtcc tgcgcagcct ccggttactc tgagcgtgtg gccaccctct accgctgcaa
     1261 ggctccgacg gatctggtct gccgctgtgg caaggtcgcc cgccggctcc ggtcggccgg
     1321 catcgactac gacgaggtcc gcgtgccgtt cctgaagcgc gaccggcccg aggtcgagga
     1381 gctgacgggc caacgctggg tgccggtgct ggtgcacggg gagcaggtca tccacgagtc
     1441 gcaccggatc ctggaatacc tggaccacgt cgcgggtcag acggcgcccg ccgacacccg
     1501 cacgggagcg ccgacctgat ggcgcagggc gcgggcaagg gcacccggct ggcgcgcagc
     1561 cggtccgccg cacagcgtgc ctggccggtg gtgctgatgg cctgggagcg ctggcagtcc
     1621 ctctccgacg aggagaagga ccgctacaag cgccaggccc gcgagtacgc cgagcgcggc
     1681 cgcaaggcgt tcgtccaggc gaagcgccgt gggcccggcc ggcactagcg ccgccgtgga
     1741 ttccgcggcg ctggccgacg cggcccgcgc gggcgcgggg ttcgccgacg accagcgcag
     1801 gctggccccc gacgtggccg gcggcctgcg cgacagcggc ctgctgcgga tgctggtccc
     1861 cgcctcgctg ggcgggggcg aggtggcgcc cgagcgcatg gtcgccgacg tcgaggcact
     1921 ggcccgggcc gacggcgccg ccggctggtg cctggccgtg gccgccaccg gaggcgtcct
     1981 ggcggcgttc atggagcccg aggccgcacg cgaggtgttc ggcgacgcgc gcggatgggt
     2041 ggccggggtg ttcgccccga agggccgcgc ggtggccacc gccgacggcg acctgaccgt
     2101 gagcgggcgg tggccgttcg ccagcggctc ggcgcacagc gactggctga tgggcggctg
     2161 tctggtgcag gagggcgacg acgtccgccg cagcgccagc ggcgcgcccg acgtgcgcct
     2221 ggccctgatg cccaccgccg cgaccacgtc cgtggacacc tggtcggtgg ccgggctgcg
     2281 cggcacgggg agcaacgact tcgaggtgga cggcgtggtg gtgccgcagg cgcgcacggt
     2341 ctcgctggtg tccgacacgg cgcgcgagcc gggcccgctc tacgccttcc ccgccttcgg
     2401 cctgctggcg ctggccatcg gcgcggtcgg gctgggcatc gcccgcggcg cggtcgacga
     2461 cctggtggcc atggcctccg ggaaggtgcc cgcgatggcc accaagaccc tggccgacca
     2521 gcccgacacc cagatccgcg tcgcccgcgc cgaggcgtcg ctgcgcgccg cgcgcgcgta
     2581 cgtggacacc gccgtcggcg aggcctggga gctggccgtc cagcaccacg acgtcgacct
     2641 cgagcggcgc gccggcctgc gcctggccgc cagccacgcc atgaccacgg cggccggggt
     2701 ggtcgacgag atgtactcgc tgggcggcgg gagctcggtc tacgagtcca gcccgctcca
     2761 gcgccgccac cgcgacatcc acgtggcgac gcagcacatg ctggtcaacc cgtccacgtg
     2821 ggagttcgct gggcgcgtgc tgctcggggc gccggtgcgg tcggagcagt tctagggcgc
     2881 cctcggcgtt cttccgtcgg cctcccctgc gggctcggcg aaacggctcg gctgggtagg
     2941 gcggacggtt ctgtgaccgt tcgcgctctt ccgtaacccg ctttgggacg cgcggcggat
     3001 ttctatcgct cagcggga
//
LOCUS       DASUPW010000432         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_653589, whole genome shotgun sequence.
ACCESSION   DASUPW010000432 DASUPW010000000
VERSION     DASUPW010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653589"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VFY44_09740"
     CDS             <1..701
                     /locus_tag="VFY44_09740"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTP cyclohydrolase, FolE2/MptA family"
                     /protein_id="HEX5910764.1"
                     /translation="QEGLRAEVSIKARYPERKITPASGSETQEIYTLLGSAVASARGT
                     RRLIGVEAHGMTACPCAQELVTDRSRERLRADGFSDEDIERIFSDVPVATHNQRGIGR
                     LYVGCPEGCTDGIEAQTLLRIVESSMSSEIYELMKRSDEVEVVERAHMHPRFVEDCVR
                     EMIRLAVEEFGQMGEDAFLLARQENLETIHRHSVVAERHGLMGELGRELREQVTVPHH
                     VTRREWLEDRPLLG"
     gene            793..1275
                     /locus_tag="VFY44_09745"
     CDS             793..1275
                     /locus_tag="VFY44_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910765.1"
                     /translation="MESAESTTSEDVVPSPAALSGDAALVVGLAATAMPFAHTAEDQA
                     ESWLRTLRLHGSVGTALSALGMSEEQLLTRAMPRSESVGTPVPEGDVMERVVRSAMEF
                     TIARGGQTTGTGDLLFALFDVYGRTMDRAMFMHGLTRSQVFEALAEADRPMSVRRSTD
                     "
     gene            complement(1272..1841)
                     /gene="pgsA"
                     /locus_tag="VFY44_09750"
     CDS             complement(1272..1841)
                     /gene="pgsA"
                     /locus_tag="VFY44_09750"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX5910766.1"
                     /translation="MFPLNLPNVLTLLRILLVPVLVVALTEEIDNGSTIAAIVFVVAS
                     LSDGLDGYIARSRQSVTTFGKVMDPIADKLLIVAALFSLVSLDRVDAWVAMVVVAREF
                     AVSGLRVAAGQQGVVIPASGLGKVKTIVQSAAVLALIAASDAGDWWVQALVYLMVLAT
                     VVSGADYFLNFRRRLEELSRQSAAPSETT"
BASE COUNT          318 a          706 c          713 g          269 t
ORIGIN      
        1 gccaggaggg cctccgggcc gaggtctcga tcaaggcccg ctaccccgag cgcaagatca
       61 cgcccgcctc cggctccgag acgcaggaga tctacacgct gctgggctcc gcggtggcgt
      121 cggcccgcgg cacccgccgc ctgatcggcg tcgaggccca tggcatgacc gcgtgccctt
      181 gcgcccagga gctggtgacc gaccgctcac gcgagcgcct gcgcgccgac ggcttctcgg
      241 acgaggacat cgagcggatc ttcagcgacg tgccggtggc cacccacaac cagcgcggca
      301 tcggccggct gtacgtgggc tgccccgagg gctgcaccga cggcatcgag gcccagacgc
      361 tgctgcggat cgtggagagc tcgatgagct cggagatcta cgagctgatg aagcgcagcg
      421 acgaggtcga ggtggtcgag cgcgcgcaca tgcacccccg cttcgttgag gactgcgtcc
      481 gcgagatgat ccggctggcc gtcgaggagt tcggccagat gggagaggac gcgttcctgc
      541 tggcccgcca ggagaacctg gagacgatcc accggcacag cgtggtcgcc gagcgccacg
      601 ggctgatggg cgagctgggc cgcgagctcc gcgagcaggt cacggttccg caccacgtga
      661 cccgccgcga gtggctcgag gaccggcccc tgctgggcta gatcgcccat tcccctgtgt
      721 ggggggtcga ccgaatggcc aggttttggc gcgttcctgt cggaaccatc cggtaccccc
      781 gaaggggtgt tcatggagtc tgctgaatcg accacgtccg aggacgtcgt gcccagtccc
      841 gccgccctga gcggcgacgc cgcgctcgtc gtcggcctgg ccgccaccgc aatgcccttc
      901 gcccacaccg ccgaggacca ggccgagagc tggctgcgca cgctccgcct gcacggaagc
      961 gtgggcaccg ccctgtccgc cctgggcatg agcgaggaac agctgttgac ccgggcgatg
     1021 ccccggagcg agagcgtggg caccccggtg cccgaaggcg acgtgatgga gcgcgtggtg
     1081 cgctcggcga tggagttcac gatcgcccgc ggcggccaga ccaccggcac cggcgacctg
     1141 ctgttcgccc tcttcgacgt ctacgggcgc acgatggacc gcgcgatgtt catgcacggc
     1201 ctgacgcggt cacaggtctt cgaggcgctg gccgaggccg accggccgat gagcgtcagg
     1261 cgctcgaccg actaggtcgt ctcactcggc gccgccgact ggcgcgacag ctcctcgagc
     1321 cggcgccgga agttcaggaa gtagtcggcg cccgagacca cggtggccag caccatcagg
     1381 tagacgaggg cctggaccca ccagtcgccc gcgtccgagg cggcgatcag cgccagcacc
     1441 gcggcggact gcacgatggt cttgaccttg cccagcccgc tggcggggat gaccaccccc
     1501 tgctggcccg cggccacgcg caggccgctg accgcgaact cgcgcgccac gaccaccatg
     1561 gccacccagg cgtcgacgcg gtccaggctg accagcgaga acagcgcggc cacgatcagc
     1621 agcttgtcgg ctatggggtc catgaccttg ccgaacgtgg tgaccgactg gcgcgagcgc
     1681 gcgatgtagc cgtccaggcc gtccgagagc gaggcgacca cgaagacgat ggccgcgatc
     1741 gtggagccgt tgtcgatctc ctcggtcagg gcgaccacca gcacgggcac caggaggatg
     1801 cgcagcagcg tcaggacgtt gggcaggttc agggggaaca tggcgaggtc taggcgaggc
     1861 tacccccgga tgcgctcgga gtgacgcgct gccagctcgg cggcctggcc aaggaactcg
     1921 gagatcgcgg ccagctgctc ggccgtgaag cgcgcgccga gctcgcgctc ccagtccgcg
     1981 gcgatggggc cccatacctc ttccgc
//
LOCUS       DASUPW010000433         4396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_930933, whole genome shotgun sequence.
ACCESSION   DASUPW010000433 DASUPW010000000
VERSION     DASUPW010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930933"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VFY44_09755"
     CDS             complement(<1..724)
                     /locus_tag="VFY44_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910767.1"
                     /translation="MQALRRSLAALAAAAALLCVAVPAAQAGTVLRVDGSSVTRAYDP
                     SVPARGAAELGRMPAGPPPAAGTAQTAKPKKKKKKGPTRGEKAVDKALKTALRKRSIS
                     KKTEASYRKTYRTARLRRDRLKGQRRKELNYVVTTLEAIALRRQLNASRMKPLFLIVQ
                     RNAQFWLRSPFPASRGYVQFRGSQMLFEYYVGEGLQLQPLANFKKANALHGACVKPTA
                     GVACNRAALARLLDEMVATRVRR"
     gene            804..1928
                     /locus_tag="VFY44_09760"
     CDS             804..1928
                     /locus_tag="VFY44_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910768.1"
                     /translation="MPLPSTKSLLRRRVAVLLLGLAVLPVAAAGCGGGGDDGGSDDAT
                     ALIEKAFGNPIKSADVKLQAELKVQGLQGLNDTPIQLSAAGPYIGGKDKLPQLDMDVK
                     LGARGQGQSLETGILSTGDRAYVKFGGEYYEQPKANVDAANRDLAKQPKGQKNALGVD
                     PGSWVTDAKTEGDEKVGGVETTHVSARVDVKKLLADLNDLARKGAGAVGGTSAPQPLT
                     EKQLSDAAKTVKDPTFDVYVAKDDETIRRMSGNLSLAVPEGEQSQTNGITGGSLRFTL
                     EMTDVNGDQKVEQPAKSRPLSDLSRQLGGAAALGGALGGGQGGGSGTTTTPQTPTTPD
                     STGGAGGANDPDAIRRYIACLDRTSPSDNAARERCRSELQ"
     gene            1976..3028
                     /locus_tag="VFY44_09765"
     CDS             1976..3028
                     /locus_tag="VFY44_09765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HEX5910769.1"
                     /translation="MELNAIAPYAGLIVALVFFLWLDLHFFARGREPNFKEAVWWSIG
                     WLVLSLAAGLVVLVLEDGEHAVLYTTVYLIERSLSLDNLFVFILLFTYFGVPEIRRAR
                     LLFWGIVAALALRGVFILAGITLIEQFHFIIYLLGLALLFLAYRIFAGVGENVDPDKN
                     LMVRAVRKVFPVTSEFHEGHWFVKQKGQRYVTPLFLCLVAIVFADIAFAIDSIPAAFA
                     ITRDGFLIWMGNVFALLGLRALFVLVEGLIARFRYLDQTIAVVLGVVGIKLLAEDLYK
                     VGPVLSLVIIFVLFAVGIALSLWADSRDPDAEAKREERKENLQPSEEPAPDESDSDEP
                     EPEEPESEPATPPGAS"
     gene            complement(3006..3329)
                     /locus_tag="VFY44_09770"
     CDS             complement(3006..3329)
                     /locus_tag="VFY44_09770"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5910770.1"
                     /translation="MSLPVDDREQVFDALADPTRRRMLSVLGERESATATELAADLPV
                     TRQAVAKHLGALAAVGLVEPTREGREVRYRLTPEPLSGAMAWMATVGGRWDTRLDDLR
                     KRLGE"
     gene            complement(3326..3667)
                     /locus_tag="VFY44_09775"
     CDS             complement(3326..3667)
                     /locus_tag="VFY44_09775"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX5910771.1"
                     /translation="MRQEVEREIELDAEPAEVWRELTESDWLGDDPVIELEPGGDLEF
                     TDRDEGRRTGWVEAVEPERRLTVWWSGDGDQESSRVEFEIVERGDGSALRVTETRPLA
                     DLTVPTAMALA"
     gene            3738..4358
                     /locus_tag="VFY44_09780"
     CDS             3738..4358
                     /locus_tag="VFY44_09780"
                     /inference="COORDINATES: protein motif:HMM:NF009205.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX5910772.1"
                     /translation="MPGLVPMVVEQTARGERSFDIYSRLLNERIVFIGQQFDDDLANV
                     VVAQMLHLAAEDPDKDVSIYVNSYGGSVTAALAIYDTMQYVPCDVQTICFGVAMSAGS
                     LVVMGGAEGKRLALPNARLLIHQPLGGFQGQSADIEIHAKEMLETRRRLEELYAQHTG
                     QSAEQLRADMDRDRFFSPREAIEYGLIDRVVERQELRRRPKGFEAG"
BASE COUNT          626 a         1554 c         1562 g          654 t
ORIGIN      
        1 cgcgccgcac gcgcgtggcc accatctcgt cgagcagccg ggccagggcg gcgcggttgc
       61 aggccacgcc ggcggtgggc ttcacgcacg cgccgtgcag cgcgttggcc ttcttgaagt
      121 tggccagcgg ctggagctgc aggccctcgc cgacgtagta ctcgaacagc atctggctgc
      181 cccggaactg cacgtagccg cggctggccg ggaacgggct gcgcagccag aactgggcgt
      241 tgcgctgcac gatcaggaac agcggcttca tgcgcgaggc gttcagctgg cggcgcaggg
      301 cgatggcctc cagcgtggtg accacgtagt tcagctcctt gcggcgctgg cccttgagcc
      361 ggtcgcggcg caggcgggcg gtgcggtacg tcttgcggta ggaggcctcg gtcttcttgg
      421 agatgctgcg tttgcgcagc gcggtcttca gcgccttgtc cacggccttc tccccgcggg
      481 tcgggccctt cttctttttc ttcttgggct tcgcggtctg ggccgtgccg gcggccgggg
      541 gcggcccggc gggcatgcgg cccagctcgg ccgcgccgcg ggcgggcacg gacgggtcgt
      601 acgcgcgcgt gacggaggat ccgtccacgc gcagcacggt gccggcctgg gcggcgggga
      661 cggccacgca cagcagcgcg gcggccgccg cgagggccgc cagggagcgt cgaagagcct
      721 gcatctggga cccccaacgc ccgggggccg cggaagttgc gtggtgcagg ccttctgtcg
      781 ctagtctgcg cccccttccg ctgatgccgc tgccgtccac gaagagcctc cttcgacgcc
      841 gcgtcgccgt gctcctgctc ggcctcgccg tgctgcccgt ggccgccgcc ggatgcggtg
      901 ggggcggcga cgacggaggc agcgacgacg cgacggcgct gatcgagaag gccttcggca
      961 acccgatcaa gagcgcggac gtgaagctgc aggccgagct caaggtccag ggcctccagg
     1021 gcctgaacga caccccgatc cagctgtcgg cggcggggcc ctacatcggc ggcaaggaca
     1081 agctcccgca gctggacatg gacgtgaagc tgggcgcgcg cggccagggc cagtcgctgg
     1141 agacgggcat cctctccacg ggagaccgcg catacgtgaa gttcggcggc gagtactacg
     1201 agcagccgaa ggccaacgtg gacgccgcca accgggacct ggccaagcag ccgaagggcc
     1261 agaagaacgc cctcggcgtg gaccccggct cctgggtcac cgacgcgaag accgagggcg
     1321 acgagaaggt cggcggcgtg gagaccacgc acgtgtcggc ccgcgtcgac gtgaagaagc
     1381 tgctggccga cctgaacgac ctggcgcgca agggcgccgg cgccgtgggg ggcaccagcg
     1441 cgccccagcc cctgaccgag aagcagctct cggacgcggc caagaccgtc aaagacccca
     1501 ccttcgacgt ctacgtggcc aaggacgacg agacgatccg gcgcatgtcg gggaacctgt
     1561 cgctggccgt ccccgagggc gagcagtcgc agaccaacgg catcaccggc gggtcgctgc
     1621 ggttcacgct cgagatgacc gacgtcaacg gcgaccagaa ggtggagcag cctgcgaagt
     1681 cgcgcccgct ctcggacctc tcgcgccagc tcggcggcgc ggcagcgctg ggcggcgccc
     1741 tggggggcgg ccagggcggc ggctcgggca cgaccaccac gccgcagacc ccgaccacgc
     1801 ccgacagcac gggcggtgcc ggcggggcca acgaccccga cgccatccgg cgctacatcg
     1861 cctgtctgga ccgcacgagc ccgagcgaca acgcggcgcg cgagcgctgc cgctccgagc
     1921 tccagtgaca cccggctggt tcaagccgga cttcgcccgc tagcgtcacc cacccgtgga
     1981 actgaacgcc atagccccgt acgcgggcct gatcgtggcg ttggtgttct tcctgtggct
     2041 ggacctgcac ttcttcgcgc gcggccggga gccgaacttc aaggaggcgg tctggtggtc
     2101 gatcggctgg ctcgtgctca gcctggcggc cggcctggtg gtcctggtgc tcgaggacgg
     2161 cgagcacgcc gtgctctaca ccacggtcta cctgatcgag cgctcgctct cgctggacaa
     2221 cctgttcgtg ttcatcctgc tgttcacgta cttcggcgtg cccgagatcc ggcgcgcgcg
     2281 gctgctgttc tggggcatcg tggccgccct ggcgctgcgc ggcgtgttca tcctggccgg
     2341 gatcacgctg atcgagcagt tccacttcat catctacctg ctcggcctgg cgctgctgtt
     2401 cctggcctac cggatcttcg ccggcgtggg cgagaacgtc gacccggaca agaacctgat
     2461 ggtgcgggcg gtgcggaagg tgttcccggt gacctccgag ttccacgagg gccactggtt
     2521 cgtcaagcag aagggccagc gctatgtgac cccgctgttc ctgtgcctcg tggccatcgt
     2581 cttcgcggac atcgcgttcg ccatcgactc catcccggcc gcgttcgcca tcacccgcga
     2641 cggcttcctg atctggatgg gcaacgtgtt cgccctcctg ggcctgcgcg cgctgttcgt
     2701 gctggtcgag ggcctgatcg cgcgcttccg ctacctcgac cagaccatcg ccgtggtgct
     2761 gggcgtggtg ggcatcaagc tgctggccga ggacctgtac aaggtcgggc cggtcctgag
     2821 cctggtgatc atcttcgtgc tcttcgccgt gggcatcgcc ctctcgctgt gggccgacag
     2881 ccgcgatccc gacgccgagg ccaagcgcga ggagcgcaag gagaacctgc agccctccga
     2941 ggagccggcg cccgacgagt cggactccga cgagccggag cccgaggagc cggagtccga
     3001 gccggctact cccccaggcg cttcctgagg tcgtccagcc gggtgtccca gcgcccgccg
     3061 acggtggcca tccacgccat cgcgcccgac agcggctccg gggtcagccg gtagcgcact
     3121 tcgcggccct cgcgggtcgg ctcgaccagc ccgacggccg ccagcgcgcc caggtgcttg
     3181 gccaccgcct ggcgggtcac gggcaggtcg gcggccagct cggtggccgt ggcgctctcg
     3241 cgctcgccca gcacgctgag catccgccgc cgcgtggggt cggccagggc gtcgaagacc
     3301 tgctcgcggt cgtcgaccgg gaggctcacg ccagcgccat cgccgtgggg acggtgaggt
     3361 cggccagcgg ccgggtctcg gtcacccgca gcgcgctgcc gtcgccgcgc tcgacgatct
     3421 cgaactccac ccgcgaggac tcctggtcgc cgtcgccgct ccaccacacg gtcaggcggc
     3481 gctcgggctc gaccgcctcg acccagccgg tgcgccggcc ctcgtcgcgg tcggtgaact
     3541 ccaggtcgcc gccgggctcg agctcgatca ccgggtcgtc gcccagccag tcggactcgg
     3601 tcagctcacg ccacacctcg gcgggctccg cgtccagctc gatctcgcgt tcaacttcct
     3661 gtcgcatgtg caactcctcg gttgcgctac agtgatgagc aactgacggg ttgcacgata
     3721 gcgaggaggc ggaggacatg cccggactgg ttcccatggt ggtcgagcag acggcgcgcg
     3781 gcgagcgctc gttcgacatc tactcacggc tgctcaacga gcggatcgtg ttcatcggcc
     3841 agcagttcga cgacgacctg gccaacgtgg tcgtggccca gatgctgcac ctggcggcgg
     3901 aggatcccga caaggacgtc tccatctacg tgaactccta cggcggctcg gtcacggccg
     3961 ccctggccat ctacgacacg atgcagtacg tcccgtgcga cgtgcagacc atctgcttcg
     4021 gggtcgccat gtcggcgggc tcgctggtgg tgatgggcgg cgccgagggc aagcggctgg
     4081 cgctgcccaa cgcgcggctg ctgatccacc agccgctcgg cgggttccag ggccagtcgg
     4141 ccgacatcga gatccacgcc aaggagatgc tcgagacccg gcgccggctg gaggagctgt
     4201 acgcccagca caccggccag agcgccgagc aactacgcgc cgacatggac cgcgaccgct
     4261 tcttcagccc ccgggaggcc atcgagtacg gcctgatcga ccgcgtggtc gagaggcagg
     4321 agctgcgccg gcggccgaag ggcttcgagg cggggtaggg gttctaccgc aggcgctacc
     4381 tgcggcctcg gcgaaa
//
LOCUS       DASUPW010000434         2801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_2040634, whole genome shotgun sequence.
ACCESSION   DASUPW010000434 DASUPW010000000
VERSION     DASUPW010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2040634"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VFY44_09785"
     CDS             complement(<1..530)
                     /locus_tag="VFY44_09785"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5910773.1"
                     /translation="MRALVTGGSGFIGSNLVDALVERGDTVAVLDNLSTGRRQNLDGA
                     LERGAVLFETDVRDPEAVASVVAETTPEVVFHMAAQIDVRRSVSDPAFDMQTNVGGTI
                     NVLEAARGAGVRRVVNTSTGGAIYGECKIIPAPEDHPVAPESPYGLSKHCAEGYCDLY
                     QRLHGVSTVSLRYSNV"
     gene            584..>2801
                     /gene="recJ"
                     /locus_tag="VFY44_09790"
     CDS             584..>2801
                     /gene="recJ"
                     /locus_tag="VFY44_09790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX5910774.1"
                     /translation="MWTTDPYDCTAAAALQDALGLGPAAAAILVRRGYADVGSAREFL
                     AADTRTDPSDLPGAAEACELVLRHVQAGSRILVFGDYDVDGVCSTAMMIRTLRALGAD
                     PAWQLPSRAEGYGLGVQAVERIAGYRTALLITVDCGVTSVAEVARARELGMDVLVTDH
                     HRPGAELPDCPVVHPALGPDGLDGPAGELCAAGVVLKLSGALYAAAGRDPALAEQDMD
                     LAGLATVCDMVPLRGENRRIARQGMDAVARTRKAGLRALMAVADLEPGDVDAQSAGFR
                     LGPRINAAGRLLRADAALELMLTDDPERATEIARELDGLNHDRRETEQRILHEAEALC
                     VPQLHAGALVVAGRDWHAGVVGIVASRLVERFRRPAVVIALDGDSGRGSGRSIGAYDL
                     HGGLGAASEHLAGFGGHRMAAGLEIEAGRVDAFRAALAAHAGAALAPADLAPVEHVDA
                     VISGGVLTLDTAEELARLGPFGPGNPKPRLLVPAAGIEYVTAMGEERKHARFSLVGGG
                     ARARGVAFRRSQRSLAAAGACPQDVVVDLERNRWNGALEARVVLRSLSPTRAGRLHEV
                     GERPLWDRIAAELAADPGRWWPPAPAAPGGRTLCERQGEGLAGLAGDLLTSGEPVLVV
                     VADVARRRAGLERTVAGMAPDGLAVASWDALGHAPRLAGPYTHLLVVDPPPVAEAMDA
                     LAAAPGPGFAHLAWGPAETEFSLAYWRHQLSLRDELAVLWRALSRHGGLDGPELEAA"
BASE COUNT          300 a         1057 c         1091 g          353 t
ORIGIN      
        1 tagacgttgc tgtagcgcag cgagaccgtc gagacgccgt gcagtcgctg gtagaggtcg
       61 cagtacccct cggcgcagtg cttggacagg ccgtagggcg actccggggc gacggggtgg
      121 tcctcggggg cggggatgat cttgcactcg ccgtagatgg cgccgccggt cgacgtgttc
      181 accacgcgcc tgacgcccgc gccgcgggcg gcctcgagca cgttgatggt gccgcccacg
      241 ttggtctgca tgtcgaacgc ggggtcggac accgaccgcc gcacgtcgat ctgggccgcc
      301 atgtggaaca cgacctcggg cgtggtctcg gccacgacgg aggccacggc ctcgggatcg
      361 cggacgtcgg tctcgaacag caccgcgccg cgctcgaggg cgccgtccag gttctggcgc
      421 ctcccggtcg agaggttgtc caggaccgcg accgtgtcgc cccgctcgac cagggcgtca
      481 acgagattgg agccgatgaa gccggacccg ccggtgacca gtgcgcgcat gggtcggcac
      541 cctagtggtc cttccgtccc ccctgcaggc cacgatggcc ctcgtgtgga ccaccgaccc
      601 ctacgactgc acggcggcag ccgcgctcca ggacgcgctc ggcctgggcc ccgccgccgc
      661 ggccatcctg gtccgccggg ggtacgccga cgtcgggtcc gcgcgggagt tcctggccgc
      721 cgacacccgt accgacccgt ccgacctgcc gggggcggcc gaggcctgcg agctggtcct
      781 gcgccacgtc caggcgggct cgcggatcct ggtcttcggc gactacgacg tggacggcgt
      841 ctgctccacg gccatgatga tccggacgct gcgcgcgctg ggcgccgacc ccgcctggca
      901 gctgcccagc cgggccgagg gctacggcct gggcgtccag gcggtggagc ggatcgcggg
      961 gtaccgcacg gccctgctca tcaccgtcga ctgcggggtc acgtcggtgg ccgaggtggc
     1021 gcgggcccgc gagctgggca tggacgtgct ggtcaccgac caccaccgcc cgggcgccga
     1081 gttgcccgac tgcccggtcg tgcacccggc gctgggccct gacggcctcg acggcccggc
     1141 gggcgagctg tgcgccgcgg gcgtcgtgct caagctctcg ggcgccctgt acgccgcggc
     1201 gggccgtgat cccgccctgg ccgagcagga catggacctg gccggcctgg ccaccgtctg
     1261 cgacatggtc ccgctgcggg gcgagaaccg gcggatcgcg cgccagggca tggacgccgt
     1321 ggcccgcacg cgcaaggcgg gcctgcgggc gctgatggcc gtggccgacc tggagcccgg
     1381 tgacgtcgac gcccagtccg cgggcttccg gctgggcccg cgcatcaacg cggcgggccg
     1441 gctgctgcgc gccgacgccg cgctggagct gatgctcacc gacgaccccg agcgggcgac
     1501 cgagatcgcc cgcgagctgg acgggctcaa ccacgaccgg cgcgagaccg agcagcggat
     1561 cctgcacgag gccgaggcgc tgtgcgtccc gcagctccac gccggggcgc tggtggtggc
     1621 cgggcgggac tggcacgcgg gggtggttgg catcgtggcc tcgcggctgg tggagcgctt
     1681 ccgccggccg gcggtggtca tcgcgctgga cggcgactcc ggtcgcgggt cggggcgcag
     1741 catcggcgcg tacgacctgc acggcggcct gggggcggcg tccgagcacc tggccggctt
     1801 cggcggccac cggatggccg ccgggctgga gatcgaggcc gggcgcgtgg acgccttccg
     1861 cgccgccctg gccgctcacg ccggcgccgc cctggcgccc gcggacctgg ccccggtcga
     1921 gcacgtcgac gccgtgatct ccggcggcgt cctgaccctg gacacggccg aggagctcgc
     1981 ccgcctgggg ccgttcggcc ccgggaaccc caagccgcgc ctgctggtgc ccgcggccgg
     2041 catcgagtac gtgaccgcca tgggcgagga gcgtaagcac gcgcgcttct cgctggtcgg
     2101 cggtggcgcc cgcgcccgcg gcgtggcctt ccgccggtcc cagcgctccc tggccgcggc
     2161 cggcgcctgc ccgcaggacg tcgtggtcga cctcgagcgc aaccgctgga acggggccct
     2221 cgaggcgcgc gtggtgctgc gctcgctcag cccgacccgc gccggccggc tgcacgaggt
     2281 gggcgagcgg ccgctgtggg accgcatcgc cgccgagctg gccgccgacc ccgggcgctg
     2341 gtggccgccg gcgcccgccg cccccggcgg ccgcacgctg tgcgagcgcc agggggaggg
     2401 gctggccggc ctggccggag acctgctgac cagcggcgag cccgtcctgg ttgtggtggc
     2461 cgacgtcgcg cgccgccgcg ccgggctgga gcggaccgtc gcgggcatgg cccccgacgg
     2521 cctggccgtg gcctcctggg acgcgctcgg ccacgccccc cggctggccg gcccgtacac
     2581 ccacctgctg gtcgtggacc cgccgccggt ggcggaggcc atggatgcgc tcgcggccgc
     2641 gcccggcccc gggttcgcgc acctggcctg ggggcccgcc gagacggagt tctcgctggc
     2701 gtactggcgc catcagctgt cgttgcgcga cgagctcgcc gtgctctggc gcgcgctcag
     2761 ccggcacggc gggctggacg ggcccgaact ggaggccgcc c
//
LOCUS       DASUPW010000435         4799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_654413, whole genome shotgun sequence.
ACCESSION   DASUPW010000435 DASUPW010000000
VERSION     DASUPW010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654413"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..1122)
                     /locus_tag="VFY44_09795"
     CDS             complement(223..1122)
                     /locus_tag="VFY44_09795"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5910775.1"
                     /translation="MTATETSRERFADVGGGITLCYETFGDPSDPPLLLVMGLGMQLL
                     GWDERFCSMLAERGFHVVRFDNRDAGLSTSVDAPVPGLRQLAVRRFDPRQYDVGDMAG
                     DAVGLLRELDLAPAHVVGASLGGMIAQTMAARSPGSVRTLTSIMSTTGHRLKGQPAPS
                     RYRFLLRPAPRERDAYMDHTVELWRAIGSPGFPADEAAVRERAARSFDRARNPAGTGR
                     QMAAVLKSGDRTREVRRITAPTLVVHGTKDTLVRPSGGTATAEAIGGAELMMIDGMGH
                     DMPPQVWPRIVDAITGRAQRFDS"
     gene            complement(1119..1592)
                     /gene="greA"
                     /locus_tag="VFY44_09800"
     CDS             complement(1119..1592)
                     /gene="greA"
                     /locus_tag="VFY44_09800"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX5910776.1"
                     /translation="MSETHKLTAEAAERLETEVTRLEGEGRREIAERIGIAREWGDLK
                     ENAEYHAAKEEAAMLEAKIARMRDQLRTSEIVEAAADGDGAGMYSAVTYADSKAGKEM
                     TFTLVPATEADPSQKRISIDSPVGQALAGASEGDKRTLETPGGERAITIVKVEQS"
     gene            1657..3048
                     /locus_tag="VFY44_09805"
     CDS             1657..3048
                     /locus_tag="VFY44_09805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX5910777.1"
                     /translation="MLKNRLSALDATFLSLEDDDSNMCVGGADVFAGPAPDQDEVLDE
                     LGRCLEAVPNFRLRRVRVPLGLGRPRWADAEDFRLEDHVSRVVLDEPGGAERVQALFA
                     HLMAEPVDHDRPPWEIQLIEGLEGGRFALLHKMHHALVDGIASVEVLQTLFSPDPGHG
                     ADVEPRTLEPKPPPSQVRMLAESVVERTLAPEALGAVAGAVTHPRRTAGSVVKGAAGV
                     AGLARAALRPAPDTPYNQPVGPERSFAWQRESLDQVKAVKDALGGSVNDVVLTAVAGA
                     LDRDLRRRGGHPGADEMYAFVPVSLRGDSGDGQLGNEVSGLKVALPLGEDDPLERFAR
                     VHQTMEALKGSPQALGASAAVGATGLVSAAAFEAMSPVGDVQRYANLVITNVPGPPDP
                     LYFLGRELEDVFPFVPLGANLAMGVAVVSYAGSLGFGFSADPATVPDVHELAPALHES
                     LAELVAAAPVASK"
     gene            3058..4203
                     /locus_tag="VFY44_09810"
     CDS             3058..4203
                     /locus_tag="VFY44_09810"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEX5910778.1"
                     /translation="MNDIVVFGATGFTGKLTAQYLAGALPEGASWALAGRNRAKLEAV
                     AADLDGAVAPELVEADVTDVASVSRVAESARVAITTVGPYLKHGDPLVAACAAAGTDY
                     VDLTGEPEFVDRSYVRHHAAAEKSGARLVHACGFDSIPHDLGARFTVEQLPEGVPLKV
                     EGFVRAGGTFSGGTYHSALGQFARARQMISAASERKKAEPTPTDGRRSRGIKGKPHRE
                     PAVNGWAVPMPTIDPQIVVRSGRALERYGPDFSYGHYAAVKRLPFALGGMAGVGALMG
                     AAQIPPLRKQLEKLKSSGDGPSAEQRAKAWFTVTFVGEGGGKKVTTRVKGGDPGYGET
                     AKMLAESALCLAFDDLPQTAGQVTTAQAMGGPLTRRLQAAGISFDVL"
     gene            4245..>4799
                     /locus_tag="VFY44_09815"
     CDS             4245..>4799
                     /locus_tag="VFY44_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910779.1"
                     /translation="MSVPPEPTPPVLVGARTARDADEERLMWRLATVSFAVGALTAFG
                     VLALPDPDPADHGAIAVIAGVLALTGVVLAVAGPRPRLLSLLPFWGVLCVSALVAVAS
                     PLGAMPLFYLWPVLQAAYFMDRRRLVAVEMTMLVSYAVALQFSPPGLRTAYFLAVVVC
                     TSLVGAVVSHLKQRVERLVDSLDSA"
BASE COUNT          572 a         1747 c         1818 g          662 t
ORIGIN      
        1 acgtgccgcc agcaccaggc gtgctgggga tagccgtgca gcagcagcac cggcggggcg
       61 ccggggtcgc cggcctccag cacgtgcagg tccagcgggc cggagtcggc cgtgtcgatc
      121 gtgaccgtcc ggtgccgggc accggggacg tcgggcgggg ccgtgggggc gagtgcgtcg
      181 atgcgtcgat cctacggcgc ggccgggccc gggccgaggg cgctaggagt cgaagcgctg
      241 ggcgcgcccg gtgatggcgt ccacgatgcg cggccacacc tgcggcggca tgtcgtggcc
      301 catgccgtcg atcatcatca gctcggcgcc gccgatcgcc tcggcggtgg cggtgccccc
      361 cgagggccgc accagcgtgt ccttggtgcc gtggacgacc agcgtcgggg cggtgatccg
      421 gcgcacctcg cgcgtgcggt cgcccgactt cagcaccgcg gccatctggc gcccggtccc
      481 ggccgggttg cgcgcgcggt cgaacgagcg ggcggcacgc tcgcggaccg cggcctcgtc
      541 ggcgggaaag cccggcgagc cgatcgcccg ccacagctcg acggtgtggt ccatgtaggc
      601 gtcgcgctcg cgcggcgcgg gccgcagcag gaagcggtag cggctggggg cgggctggcc
      661 cttgagccgg tggccggtgg tggacatgat cgacgtcagc gtgcggacgc tgcccgggct
      721 gcgggcggcc atggtctggg cgatcatgcc gcccagcgag gcgcccacca cgtgggcggg
      781 ggccaggtcc agctcgcgca gcaggccgac ggcgtcgccg gccatgtcgc ccacgtcgta
      841 ctggcgcggg tcgaagcggc gcaccgccag ctggcgcagg cccggcaccg gagcgtcgac
      901 cgacgtggac agcccggcgt cgcggttgtc gaagcgcacc acgtggaagc cgcgctcggc
      961 cagcatcgag cagaaccgct cgtcccagcc cagcagctgc atgcccaggc ccatgaccag
     1021 cagcagcggc gggtccgacg ggtcgccgaa ggtctcgtaa cagagcgtga tcccgccgcc
     1081 gacgtcggcg aacctctcac gggaggtctc ggtcgccgtc acgactgctc gaccttcacg
     1141 atcgtgatcg cgcgctcgcc ccccggcgtc tcaagcgtgc gcttgtcgcc ctcggacgcg
     1201 ccggccaggg cctggccgac cggggagtcg atcgagatcc gcttctgcga cgggtccgcc
     1261 tccgtggcgg gcaccagcgt gaaggtcatc tccttgcccg ccttcgagtc ggcgtaggtc
     1321 acggcggagt acatgcccgc gccgtcgccg tcggcggccg cctcgacgat ctcggaggtg
     1381 cgcagctggt cgcgcatgcg cgcgatcttg gcctccagca tcgcggcctc ctccttggcg
     1441 gcgtggtact cggcgttctc cttgaggtcg ccccactcac gggcgatgcc gatccgttcg
     1501 gcgatctcgc gccgcccctc gccctccagg cgtgtgacct ccgtctcgag gcgttctgcc
     1561 gcctctgcgg tcagcttgtg ggtctcgctc atcgggcgag tgtggcagga ggtgcgccgg
     1621 ggtactccat tccccagccg gacggaggga gaggcagtgc tgaagaaccg cctgagcgcc
     1681 ctggacgcca cgttcctgtc gctcgaggac gacgactcga acatgtgcgt cggaggcgcc
     1741 gacgtgttcg cgggcccggc gccggaccag gacgaggtgc tcgacgagct gggccggtgc
     1801 ctcgaggcgg tgcccaactt ccgcctgcgg cgcgtgcgcg tgccgctggg cctcggccgc
     1861 ccgcgctggg ccgacgcgga ggacttccgg ctggaggacc acgtctcgcg cgtggtgctg
     1921 gacgagcccg gtggcgccga gcgcgtgcag gcgctgttcg cgcacctgat ggccgagccg
     1981 gtggaccacg accggccgcc gtgggagatc cagctgatcg agggcctcga gggcggtcgc
     2041 ttcgcgctgc tgcacaagat gcaccacgcg ctggtggacg ggatcgccag cgtggaggtg
     2101 ctccagaccc tgttctctcc cgatcccggc cacggcgccg acgtggagcc gcgcacgctg
     2161 gagcccaagc cgcccccctc gcaggtgcgg atgcttgccg agtcggtggt cgagcgcacg
     2221 ctggcgcccg aggcgctggg tgcagtcgcc ggcgcggtga cgcacccgcg ccggaccgcg
     2281 ggctcggtgg tcaagggcgc cgcgggggtg gccgggctgg cccgggcggc gctgcgcccc
     2341 gccccggaca cgccctacaa ccaacccgtg gggccggagc gctccttcgc ctggcagcgc
     2401 gagtcgctgg accaggtcaa ggcggtcaag gacgcgctcg gcggctcggt caacgacgtg
     2461 gtcctgaccg cggtggccgg cgcgctggac cgcgacctgc gtcgccgcgg cggccatccc
     2521 ggcgccgacg agatgtacgc cttcgtgccg gtgagcctgc gcggcgactc cggcgacggc
     2581 cagctgggca acgaggtctc cggcctgaag gtggcgctcc cgctgggcga ggacgacccg
     2641 ctggagcggt tcgcgcgcgt gcaccagacc atggaggcgc tcaagggcag cccccaggcg
     2701 ctgggggcca gcgccgcggt cggcgcgacc gggctggtct cggcggcggc cttcgaggcc
     2761 atgagcccgg tgggcgacgt gcagcgctac gcgaacctgg tgatcaccaa cgtgccggga
     2821 ccgcccgatc ccctctactt cctgggccgg gagctggagg acgtgttccc gttcgtgccg
     2881 ctgggcgcca acctggcgat gggcgtcgcc gtggtcagct acgcgggctc gctggggttc
     2941 ggcttctcgg ccgaccccgc gaccgtgccc gacgtgcacg agctcgcgcc cgcgctccac
     3001 gagtcgctgg ccgagctggt cgccgccgcg ccggtggctt ccaaataggc tggcgccatg
     3061 aacgacatcg tcgtgttcgg agccacgggc ttcaccggga agctgacagc gcagtacctg
     3121 gcgggcgccc tgccggaggg cgcctcgtgg gcgctggcgg gccgcaaccg cgccaagctc
     3181 gaggcggtgg ccgccgacct ggacggcgcc gtcgcgcccg agctggtcga ggccgacgtg
     3241 accgacgtgg cctcggtcag ccgggtggcc gagtcggcgc gggtggccat caccaccgtc
     3301 ggcccctacc tcaagcacgg cgatccgctg gtggccgcct gcgccgcggc cggcaccgac
     3361 tacgtggacc tgaccggcga gcccgagttc gtcgaccgct cctacgtgcg ccaccacgcg
     3421 gcggccgaga agtccggcgc gcggctggtg cacgcgtgcg gcttcgactc gatcccccac
     3481 gacctgggcg cgcggttcac cgtggagcag ctgcccgagg gcgtgccgct gaaggtcgag
     3541 gggttcgtcc gcgccggcgg caccttctcg ggcggcacct accactcggc gctggggcag
     3601 ttcgcccgcg cccggcagat gatctccgcc gcgtccgagc gtaagaaggc cgagcccacg
     3661 cccacggacg ggcgccgctc gcgcggcatc aagggcaagc cccaccgcga gcccgcggtg
     3721 aacgggtggg cggtgccgat gcccaccatc gacccgcaga tcgtggtccg ctccggtcgc
     3781 gcgctggagc gctacggccc cgacttctcc tacggccact acgccgccgt caagcggctg
     3841 ccgttcgcgc tcggcggcat ggccggcgtg ggcgcgctga tgggcgcggc ccagatcccg
     3901 ccgctgcgca agcagctgga gaagctcaag agctcgggcg acgggcccag cgccgagcag
     3961 cgcgccaagg cctggttcac cgtcacgttc gtgggcgagg gcggcggcaa gaaggtcacc
     4021 accagggtca aggggggcga tcccggctac ggcgagacgg ccaagatgct ggccgagtcc
     4081 gcgctgtgcc tggcgttcga cgatctgccg cagaccgcgg gccaggtgac caccgcccag
     4141 gccatggggg gaccgctgac ccggcggctg caggccgcgg ggatctcctt cgacgtgctc
     4201 tgacccggct caagtcggga cgccgtgctc ccgataccga tcgggtgagc gtcccgcccg
     4261 agcccacccc gcccgtgctg gtgggcgcgc gcacggcgcg cgacgccgac gaggagcggc
     4321 tgatgtggcg gctggcgacg gtctcgttcg ccgtcggcgc gctgaccgcc ttcggggtcc
     4381 tggcccttcc ggaccccgac ccggccgacc acggcgccat cgccgtcatc gcgggcgtcc
     4441 tggcgctgac gggcgtcgtc ctggccgtgg ccgggccgcg cccccggctg ctctcgctcc
     4501 tcccgttctg gggggtgctg tgcgtcagcg ccctggtggc ggtcgccagc ccgctgggcg
     4561 cgatgccgct gttctacctc tggcccgtgc tccaggccgc gtacttcatg gaccggcgcc
     4621 ggctggtggc cgtggagatg accatgctcg tcagctacgc ggtggcgctg cagttctcgc
     4681 cccccggcct gcgcacggcg tacttcctgg ccgtggtggt gtgcacgtcc ctggtggggg
     4741 ccgtggtcag ccacctcaag cagcgggtcg agcgcctggt ggacagcctg gactccgcg
//
LOCUS       DASUPW010000436         2891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1624276, whole genome shotgun sequence.
ACCESSION   DASUPW010000436 DASUPW010000000
VERSION     DASUPW010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624276"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1490)
                     /locus_tag="VFY44_09820"
     CDS             complement(159..1490)
                     /locus_tag="VFY44_09820"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5910780.1"
                     /translation="MLGAALLAVVTVLTVFAVGRPSNPFADRHTYYAIFDSVQGLGPI
                     DRDVRVAGAKVGKIGEVQRRGDDVRVELVLDHDVTLHRDATAAMRPHTLFEGSNFVDL
                     SPGSPSARTVDDGATIPLARTRNYVTLDKALRVLRPDIRQNLRTLAEVGSRTLRDEAV
                     VGLQQTLRTTPPLMRDLAPVTRAMRGTKGDELAGAVRGLSVTVDALQSREEDLAPLVS
                     RTNQTAAALTVDDAQPLDATLAALPPTLRELRASAPALTGVVQRSERLAGGLTPALPD
                     LTAGLREGTPVVRRTIPVARQATPLVRDARLIASRLGRARPELVEMFQLTQPSLKKFN
                     SLFEQFNAPTSLGAPTGVAQLVGGAFSGLDAVFRPYQTRAQSPTSPGHAGRLGTYVDP
                     AGLTGLVESLGLGSAAARSARRGVTEQPSCADVKGVDADAAKALRLTGGCR"
     gene            complement(1511..>2891)
                     /locus_tag="VFY44_09825"
     CDS             complement(1511..>2891)
                     /locus_tag="VFY44_09825"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5910781.1"
                     /translation="PPGLPAQMTVRPTRRAKRGGRLVRAADAASRRPMLLGVLILAVG
                     AFLLVVGFLATTGPPFQEKYRLRVTVTGEDPVLRTGQAVRIGGRLAGIISDVQPDPRR
                     GRATVTANITKPGFRPLTSDTKAYVRVHSIVYETYLELRPGRSGTELADGDRLRASAA
                     SGVDLLEAVQLFDRKVQRDLRGTVVSTGIGVAGRGRGLNAALADTPPLARDLAAQLGA
                     ATRDEGALGRIIAGASRTARGARGTRPDDVAGLITSADLTLDTVARRREDLRLAIRRL
                     PAFEGTFLTVAPEAEPLLDDVAALSNDLRPTVRGLNATMPALNGVLGNGRALRVDADR
                     LADAADPVLRLARPAVYELFPVMTTLRPLNADLRTLLTRIGPYRTEYRIPGRPGLRKG
                     TGEITQAGRRFIDATDDPVPRGLAPGAPTWRVLPVFTPRPCQNPFPKPGQAGKDTMVG
                     GACRPGGGR"
BASE COUNT          346 a         1062 c         1128 g          355 t
ORIGIN      
        1 gaactcgaac ggcgccttgg tgatcagcgg cacgcgcacg tccttcttga tggccgtgta
       61 gcccaggaag gtcagcgcgg cgatcagctg gagcgccagg atcgacttgc cgaagggcac
      121 cggccgggag cgtttggcgc gcatgcgcag cttcgccatc atcggcagcc cccggtcagc
      181 cgcagtgcct tcgcggcgtc ggcgtcgacg cccttgacgt cggcgcacga cggctgctcg
      241 gtgaccccgc gccgggccga cctggcggcg gcgctgccca ggccgagcga ctccacgagc
      301 ccggtcagcc cggcggggtc gacgtaggtg cccaggcgcc cggcgtgacc gggcgaggtc
      361 ggagactggg cgcgcgtctg gtacgggcgg aacaccgcgt cgaggcccga gaacgcgccg
      421 ccgaccagct gggccacgcc ggtgggcgca ccgagcgatg tgggcgcgtt gaactgctcg
      481 aacagggagt tgaacttctt cagcgagggc tgcgtgagct ggaacatctc caccagctcg
      541 ggccgggccc ggcccaggcg cgaggcgatc agccgcgcgt cgcgcacgag cggcgtggcc
      601 tggcgcgcga cggggatcgt gcggcgcacg accggcgtcc cctcgcgcag gccggcggtc
      661 aggtcgggca gcgcgggcgt gaggccgccc gccaggcgct cgctgcgctg caccaccccg
      721 gtcagcgcgg gagccgacgc gcgcagctcg cgcagcgtgg gcggcagcgc cgcgagcgtg
      781 gcgtccagcg gctgcgcgtc gtccacggtc agcgccgcgg cggtctggtt ggtgcggctg
      841 accagcggcg ccaggtcctc ctcccggctc tggagcgcgt cgacggtgac cgacagcccg
      901 cggaccgccc cggccagctc gtcccccttg gtcccgcgca tggcccgggt gaccggggcc
      961 aggtcgcgca tcagcggcgg ggtggtgcgc agcgtctgct ggaggccgac gacggcctcg
     1021 tcgcggagcg tgcgcgagcc cacctcggcc agcgtgcgca ggttctggcg gatgtccggg
     1081 cgcaggaccc gcagcgcctt gtccagcgtc acgtagttgc gcgtccgggc cagcgggatc
     1141 gtggccccgt cgtcgacggt ccgcgcgctc gggctgccgg gcgacaggtc cacgaagttc
     1201 gagccctcga acagcgtgtg gggccgcatg gcggcggtcg cgtcgcggtg cagcgtcacg
     1261 tcgtggtcca gcaccagctc cacccgcacg tcgtcgccgc gccgctggac ctctccgatc
     1321 ttgccgacct tggcgccggc cacgcgcacg tcgcggtcga tggggcccag gccctggacc
     1381 gagtcgaaga tcgcgtagta ggtgtggcgg tccgcgaacg ggttcgaggg ccgcccgacc
     1441 gcgaacacgg tcagcacggt gaccaccgcg agcagggccg cgccgagcac cctccgccgg
     1501 tacgtggggt tcatcggccg cccccggggc ggcaggcccc gccgaccatg gtgtccttgc
     1561 cggcctggcc gggcttgggg aacgggttct ggcacgggcg cggggtgaac acgggcagca
     1621 cccgccaggt gggggcgccc ggggcgagcc cgcgcggcac cgggtcgtcg gtcgcgtcga
     1681 tgaagcggcg ccccgcctgg gtgatctcgc cggtgccctt gcgcaggccc ggccgcccgg
     1741 ggatgcggta ctcggtccgg tacgggccga tacgcgtcag cagcgtgcgc aggtcggcgt
     1801 tcagcgggcg gagcgtggtc atgaccggga acagctcgta gaccgcgggc cgggccaggc
     1861 gcaggaccgg gtcggccgcg tcggccagcc ggtcggcgtc cacgcgcagg gcgcgcccgt
     1921 tccccagcac accgttcagc gcgggcatgg tcgcgttcag gccccgcacg gtcggccgca
     1981 ggtcgttcga gagcgcggcc acgtcgtcca gcaggggctc ggcctccggg gccaccgtca
     2041 ggaaggtgcc ctcgaaggcg ggcaggcgcc ggatcgccag gcgcaggtcc tcgcgccggc
     2101 gcgcgaccgt gtccagcgtc aggtcggccg aggtgatcag ccccgccacg tcgtccggcc
     2161 gcgtgccgcg ggcgccgcgc gccgtccgcg aggcgccggc gatgatgcgc cccagggcgc
     2221 cctcgtcgcg cgtggccgcc cccagctgcg cggccaggtc gcgggccagc ggcggcgtgt
     2281 cggccagggc cgcgttcagg ccccggccgc gcccggcgac gccgatcccc gtgctgacca
     2341 ccgtgccgcg caggtcgcgc tgcaccttgc ggtcgaacag ctggacggcc tccagcaggt
     2401 cgacccccga ggcggcggag gcccgcaggc ggtcgccgtc ggccagctcg gtgccgctcc
     2461 ggcccgggcg cagctccagg taggtctcgt agacgatcga atggacccgg acgtaggcct
     2521 tcgtgtccga ggtcagcggc cggaaccccg gcttggtgat gttcgccgtg accgtcgcgc
     2581 ggccgcggcg gggatcgggc tggacgtcgc tgatgatgcc cgccaggcgc ccgccgatcc
     2641 ggaccgcctg cccggtgcgc aggaccgggt cctcccccgt cacggtcacg cgcaggcggt
     2701 acttctcctg gaacgggggg cccgtggtgg ccaggaagcc cacgaccagc aggaacgcgc
     2761 cgaccgcgag gatcaggacg cccagcagca tcggccgccg ggacgcggcg tcggcggcgc
     2821 gcaccagccg gccgccccgc ttcgctcgtc gtgtcggccg cacggtcatt tgagcaggta
     2881 gtccaggagg c
//
LOCUS       DASUPW010000437         5998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_379493, whole genome shotgun sequence.
ACCESSION   DASUPW010000437 DASUPW010000000
VERSION     DASUPW010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5998
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379493"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VFY44_09830"
     CDS             <1..749
                     /locus_tag="VFY44_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910782.1"
                     /translation="RGTAVVLVGHVTKEGALAGPRVLEHLVDCVLSFEGERERTYRTL
                     RALKNRFGSTNEAGVFEMREEGLVEVEDASARFVAEATRAPGSVVLASMEGSRPLLVE
                     VQALVAPTELVPPRRMANGVDRNRLALVLAVLTRHAGIALGGADVFVSVAGGVRVDEP
                     GADLAVALAVASAARGVTLGDGRAPLAAFGELGLTGELRHAAHPDRRLAEAKKFGLAS
                     VLHPGDGLPDLRTALRRGLGPADASPARAA"
     gene            813..1922
                     /gene="disA"
                     /locus_tag="VFY44_09835"
     CDS             813..1922
                     /gene="disA"
                     /locus_tag="VFY44_09835"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:NF010009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HEX5910783.1"
                     /translation="MAYAGDEVAELESRQEPRLVRALEMIAPGTAIREGVDAIVQGRT
                     GALICIGDNEDLSFLYSGGLKLEVDYTPALLFQLAKMDGAIILSSNGTKIGWANVQLM
                     PDPTILSLETGTRHRTAERVSKQTDALLICVSQSRSVVSLYLDGAKYILEDIPVVLAK
                     ANQALATLDKYRTRLDQVTTRLTALEFEGGVTLHDVLTVLQRGELVTRMAVEIERFIV
                     ELGAEGRLIEMQLEETLVGVAADKAALVHDYLVEPNEDSFAKVLDSLVRLAHQDLLDF
                     GRLAELLGYDRKLNTLDHPVSPRGHRVLGRIPRLPKLVVQSIVGEFGGLDEILAASDG
                     ELESVEGVGDMRAKDIREGLRRLQEINLVDRYLQT"
     gene            2050..2556
                     /locus_tag="VFY44_09840"
     CDS             2050..2556
                     /locus_tag="VFY44_09840"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX5910784.1"
                     /translation="MGDHVVYPHHGAGKVLKKEQKEVLGETREYLTIKILHNDMTVMV
                     PCENAGRAGLRRVIGEEAVNKVLGVLRDEVSEMPKNWNRRFKHNREKIKTGDVYELAE
                     VVRNLAIRENEKGLSTGEKQMYTRSKKILASELMYALEMGEDDAEDHLDSIIEDAHNV
                     RVAAAATA"
     gene            2567..3205
                     /gene="ispD"
                     /locus_tag="VFY44_09845"
     CDS             2567..3205
                     /gene="ispD"
                     /locus_tag="VFY44_09845"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX5910785.1"
                     /translation="MSLVGIVPAGGSGERLGAQRPKAFVVLAGRAMLEWSVEVLERVC
                     DRVVVAVPEGYDADDAVPGGPSRSESVRNAIQAAPEAEVYVVHDAARPLLTEELVRRC
                     VDALGDGVDGAIAAAPVTDTIKHTDGQGRVVSTLERSALWAVQTPQVFRAEALRRALD
                     ADAAALAGATDDASLVEAAGGRVDVVDSGIENFKVTTPLDLRMAEAVLAERC"
     gene            3199..4026
                     /locus_tag="VFY44_09850"
     CDS             3199..4026
                     /locus_tag="VFY44_09850"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HEX5910786.1"
                     /translation="MLTDYHVHLRPDEYDATPAEYFTAENARVYRDAATERGIAELGV
                     SEHIHRFTAALDVWQHDWWKRNARDDIDAYNAFLREAGLKVGIEADLIRGREEQTAAL
                     LDAREWDYVVGSVHFLQDEAVDMRESEWDIWESADPDEVWSRYFETLGHAASTGLFDI
                     LAHPDLVKVWGKGAPRPEGDLRRYYELAMPGIEAADVAIEVSTAGLRKPVGEIYPDAE
                     FLRMCLDLGKPVALSSDAHVPGDLGHGYGQAVAFLRENGVDQLATFTGRRRTMVPLG"
     gene            4023..4505
                     /gene="ispF"
                     /locus_tag="VFY44_09855"
     CDS             4023..4505
                     /gene="ispF"
                     /locus_tag="VFY44_09855"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX5910787.1"
                     /translation="MSTRTGIAYDSHRLDASRPLILGGVHVAEAARGLTGHSDADVLT
                     HAVIDALLGAAGLADIGHHFPDTDPEFRNADSIELLAQVRGLLDGEGLRTTYVDATVI
                     AEAPKLGPHRDAMRERLAGALGLAEGSVNVKFTTNEGMGALGRGEGMAAMAVATLEDA
                     "
     gene            4640..4975
                     /locus_tag="VFY44_09860"
     CDS             4640..4975
                     /locus_tag="VFY44_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910788.1"
                     /translation="MSQTAAVSKSSEPRSLGSWQFWLANAVFAPIAASLSLWLVSSGG
                     QDWQSGVLVTAAVFAVIYALVARAWELEPVAAVGGYLLATVLTVPWGYGLLIGALVVS
                     GETGSTCLS"
     gene            4987..5298
                     /locus_tag="VFY44_09865"
     CDS             4987..5298
                     /locus_tag="VFY44_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910789.1"
                     /translation="MNPTPWWFWLVALIVAPIIALALFVGLFAVSGWSGSGAWVAGAI
                     AIAVGGGAVIWVGEGLRIDRLWTGVGVVLASLAAPGWLFGAIVLLMAITCPEAGCFQL
                     R"
     gene            5311..>5998
                     /locus_tag="VFY44_09870"
     CDS             5311..>5998
                     /locus_tag="VFY44_09870"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX5910790.1"
                     /translation="MPEQSDRPAAQSTDVAFAGAARQAELIASGELSSRELVELYLGR
                     IERIDPQVNAYRTVMADRALTEADQADRRRAGGDERPLLGVPVAIKDTTDVAGEVSTQ
                     GTAAHGGPAAEDAELVRRLRAAGAVIIGKTNLPELAIIGATESPNWGVTRNPWDLDRT
                     PGGSSGGSAAAVAAGLAALAHATDGAGSIRIPAACCGLFGLKPSRGRVSFAPGGEGWA
                     GASIQHAVTRS"
BASE COUNT          869 a         2112 c         2168 g          849 t
ORIGIN      
        1 agcgcggcac cgcggtcgtc ctggtcggcc acgtgaccaa ggagggcgcc ctggccggcc
       61 cgcgggtgct cgagcacctg gtcgactgcg tcctgtcctt cgagggcgag cgcgagcgca
      121 cctaccgcac cctgcgcgcg ctgaagaacc gcttcggctc caccaacgag gccggggtgt
      181 tcgagatgcg cgaggagggg ctggtcgagg tcgaggacgc ctcggcccgg ttcgtggccg
      241 aggccacgcg cgcgcccggg tcggtcgtgc tggcctcgat ggagggctcg cggccgctcc
      301 tggtcgaggt ccaggcgctg gtggcgccca ccgagctggt gcccccgcgc cggatggcca
      361 acggcgtcga ccgcaaccgc ctggccctgg tgctggccgt gctcacccgt cacgcgggca
      421 tcgccctggg cggcgccgac gtgttcgtct cggtcgccgg cggcgtgcgc gtggacgagc
      481 ccggcgccga cctggcggtc gcgctggccg tggcctcggc cgcgcgcggg gtcaccctgg
      541 gcgacggccg cgccccgctg gccgcgttcg gcgagctcgg cctgaccggc gagctccggc
      601 acgccgcgca ccccgaccgg cgcctggccg aggcgaagaa gttcggcctg gcctccgtcc
      661 tccatccggg ggacgggttg ccggacctgc gcaccgcgct ccggcgcggg ctgggccccg
      721 cagacgcctc gccggcccgg gctgcctagc ttttcccgca atagagccaa atcgcggggc
      781 tttttcgggc cgagtgccgt acaatggctc ggatggccta cgcaggagac gaagttgcgg
      841 agctcgaatc taggcaagag ccccgcctcg tacgggccct cgagatgatc gccccgggca
      901 cggcgatccg tgaaggcgtc gacgccatcg tgcagggccg caccggcgcg ctgatctgca
      961 tcggcgacaa cgaggacctc tcgttcctgt actccggcgg cctcaagctg gaggtcgact
     1021 acaccccggc gctgctgttc cagctggcga agatggacgg cgcgatcatc ctgtcgtcca
     1081 acggcacgaa gatcggctgg gccaacgtcc agctgatgcc cgatcccacg atcctctcgc
     1141 tggagaccgg cacacgccac cgcaccgccg agcgcgtgtc caagcagacc gatgcgctgc
     1201 tcatctgcgt ctcgcagtca cgcagcgtgg tgtcgctcta cctcgacggc gccaagtaca
     1261 tcctcgagga catccccgtg gtgctggcca aggccaacca ggccctggcc accctggaca
     1321 agtaccgcac ccgcctggac caggtgacca cccgcctgac cgcgctcgag ttcgaaggcg
     1381 gcgtgacgct ccacgacgtg ctcaccgtgc tgcagcgggg cgagctcgtc acgcgcatgg
     1441 cggtcgagat cgagcgcttc atcgtcgagc tgggcgccga gggccgcctg atcgagatgc
     1501 agctggagga gacgctcgtg ggcgtggccg ccgacaaggc cgccctggtg cacgactacc
     1561 tggtcgagcc caacgaggac tccttcgcga aggtgctcga ctccctcgtg cgcctggccc
     1621 accaggacct cctggacttc ggccgcctgg ccgagctgct gggctacgac cgcaagctga
     1681 acacccttga ccatcccgtg tcaccacggg gccaccgcgt cctgggccgc attccgcggc
     1741 tccccaagct cgtcgttcag agcatcgtgg gcgagttcgg tgggctggac gagatcttgg
     1801 ccgcatccga cggagagctg gagtccgtcg agggagtggg ggacatgcgc gccaaggaca
     1861 tacgcgaggg cctgcgcagg ctgcaggaga tcaacctcgt cgataggtat ctgcaaacct
     1921 gaatagggca gccgggtgca cgacgcaacc cggctttttg actaggagga cgtcatttcc
     1981 gagacggcga ttgagcaaga gatcgaggac ctcgagatca cgcgggagat cccgcacatc
     2041 gagtacgaga tcggcgatca cgtggtgtat ccgcaccacg gcgctggcaa ggtcctcaag
     2101 aaggagcaga aggaggttct cggcgagacg cgggagtacc tcaccatcaa gatcctccac
     2161 aacgacatga ccgtgatggt cccgtgtgag aacgccgggc gcgcggggct gcgccgcgtg
     2221 atcggcgagg aggcagtgaa caaggtgctc ggagtcctcc gcgacgaggt gtccgagatg
     2281 ccgaagaact ggaaccgccg cttcaagcac aaccgggaga agatcaagac cggtgacgtg
     2341 tacgagctgg ccgaggtggt ccgcaacctg gccatccgcg agaacgagaa gggcctctcg
     2401 acgggcgaga agcagatgta cacccgctcg aagaagatcc tggcctcgga gctgatgtac
     2461 gccctcgaga tgggcgagga cgacgccgag gaccacctgg actcgatcat cgaggacgcc
     2521 cacaacgtcc gcgtggccgc cgcggcgacc gcctgatcgc agccacatgt ccctcgtggg
     2581 aatagttccc gcgggcggaa gcggcgaacg ccttggcgcc cagcggccca aggcgttcgt
     2641 cgttctggcc ggccgcgcga tgctcgagtg gagcgtcgag gtgctcgagc gcgtctgcga
     2701 ccgcgtggtc gtggcggtgc ccgagggcta cgacgccgac gatgccgtgc ccggcggtcc
     2761 ctcgcggtcg gagtccgtcc gcaacgcgat ccaggccgcg ccggaggccg aggtgtacgt
     2821 ggtccacgac gccgcacgcc cgctgctgac cgaggagctc gtgcgccggt gcgtggatgc
     2881 gctcggcgac ggcgtcgacg gcgccatcgc cgccgcgccg gtcacggaca ccatcaagca
     2941 caccgacggc cagggccgcg tggtctccac gctggagcgc tccgcgctgt gggccgtgca
     3001 gacgccgcag gtgttccgcg ccgaggcgct gcggcgcgcg ctggacgccg acgccgccgc
     3061 gctggccggg gccaccgacg acgcctcgct ggtggaggcg gccgggggcc gcgtggacgt
     3121 ggtggattct gggatcgaga acttcaaggt caccacgccg ctggacctga ggatggctga
     3181 ggcggtgctg gccgagcgat gctgaccgac taccacgtcc atcttcggcc ggacgagtac
     3241 gacgccaccc cggccgagta cttcaccgcc gagaacgccc gcgtctaccg ggacgccgcg
     3301 accgagcgcg gcatcgccga gctgggcgtc tccgagcaca tccaccgctt cacggccgcg
     3361 ctggacgtct ggcagcacga ctggtggaag cgcaacgccc gcgacgacat cgacgcctac
     3421 aacgccttcc tgcgcgaggc cggcctgaag gtgggcatcg aggccgacct gatccgcggc
     3481 cgcgaggagc agacggccgc cctgctggac gcgcgcgagt gggactacgt ggtcgggtcg
     3541 gtgcacttcc tccaggacga ggccgtggac atgcgcgaga gcgagtggga catctgggag
     3601 tcggccgacc ccgacgaggt ctggtcgcgc tacttcgaga cgctgggcca cgccgcctcc
     3661 accggcctgt tcgacatcct ggcccacccc gacctggtca aggtgtgggg caagggcgcg
     3721 ccccggcccg agggcgacct gcgccgctac tacgagctgg ccatgcccgg catcgaggcc
     3781 gccgacgtgg ccatcgaggt ctccaccgcc gggctgcgca agccggtggg ggagatctac
     3841 cccgacgccg agttcctgcg catgtgcctg gacctgggca agccggtcgc gctgtccagc
     3901 gacgcccatg tgccgggcga cctgggccac gggtacggtc aggccgtggc gttcctgcgc
     3961 gagaacgggg tcgaccagct ggccaccttc accggccgcc ggcgcaccat ggtgccgctg
     4021 ggatgagcac ccgcaccggc atcgcctacg actcgcaccg cctcgacgca tcgcgcccgc
     4081 tgatcctggg cggcgtgcac gtggccgagg cggcgcgcgg gctgaccggc cactccgacg
     4141 ccgacgtgct cacgcacgcg gtcatcgacg ccctcctggg cgcggccggg ctggccgaca
     4201 tcggccacca cttcccggac accgacccgg agttccggaa cgcggactcg atcgagctgc
     4261 tcgcgcaggt gcgcgggctg ctggacggcg agggcctgcg cacgacctac gtggacgcca
     4321 cggtcatcgc cgaggcgccc aagctcggcc cccaccgcga cgcgatgcgc gagcgcctgg
     4381 ccggggccct cggcctggcc gagggctcgg tgaacgtcaa gttcaccacc aacgagggca
     4441 tgggcgccct cggccgcggc gagggcatgg ccgcgatggc cgtggccacg ctcgaagacg
     4501 cctgagcccc gcgcggcatt gagcgatggc ttcttcccgc tgggcgggga ctatccgtcg
     4561 cgcgtcctgt tgaccatcgg tcagtaggtg tgttacggtc tgctgaccga tggtcaagaa
     4621 gccgacacga ggaggagcca tgtcccagac cgccgccgtt tcgaagtcgt ccgagccgcg
     4681 cagcctcggc agctggcagt tctggctcgc caacgcggtg ttcgcgccca tcgccgccag
     4741 cctcagcttg tggctcgtct cgagcggcgg ccaggactgg cagtccggtg tcctcgtgac
     4801 cgctgccgtg ttcgccgtga tctacgcgct cgtggcccgg gcctgggagc tggaacccgt
     4861 ggccgcggtc ggcgggtatc tcctggccac ggtgctgacc gtgccctggg gatatggcct
     4921 cctgatcggc gcgcttgtcg tcagcgggga gacggggagc acgtgtctga gctgagcgga
     4981 acctccgtga accccacgcc ctggtggttc tggctcgtgg cgctgatcgt ggccccgatc
     5041 atcgccctgg cgctgttcgt cgggctcttt gccgtttccg gctggagcgg gtccggcgcc
     5101 tgggtcgcag gcgcgatcgc gatcgcggtc ggagggggcg ccgtgatctg ggtgggcgag
     5161 ggcctacgca tcgaccgctt gtggacgggc gtaggcgtgg tgctcgcctc cctggcagca
     5221 ccgggctggc tcttcggcgc gatcgtgctg ttgatggcga tcacctgccc ggaggccggc
     5281 tgctttcaac tgaggtaata cgctcggccc gtgcccgagc agtccgaccg ccccgccgcc
     5341 cagtccaccg acgtcgcctt cgccggcgcg gctcgccagg cggagctgat cgcctcgggg
     5401 gagctgagct cgcgcgagct ggtggagctg tacctgggcc ggatcgagcg catcgacccg
     5461 caggtgaacg cctaccggac ggtcatggcc gaccgggcgc tgaccgaggc cgaccaggcc
     5521 gaccggcgcc gcgccggcgg cgacgagcgg ccgctgctgg gcgtgccggt ggccatcaag
     5581 gacacgaccg acgtcgcggg cgaggtgtcc acccagggca cagccgcgca cggcgggccg
     5641 gccgccgagg acgcggagct ggtgcgccgg ctccgggccg cgggcgccgt gatcatcggc
     5701 aagaccaacc tgcccgagct ggccatcatc ggcgccaccg agtctcccaa ctggggcgtg
     5761 acgcgcaacc cgtgggacct ggaccggacg ccgggtggct ccagcggcgg cagcgccgcc
     5821 gccgtcgcgg ccggcctggc cgcgctggcg cacgccaccg acggcgcggg ctcgatccgc
     5881 atcccggcgg cctgctgcgg cctgttcggg ctgaagccgt cgcgcgggcg ggtgtcgttc
     5941 gccccgggcg gcgagggctg ggccggggcc tcgatccagc atgcggtgac gcgctcgg
//
LOCUS       DASUPW010000438         7370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_240782, whole genome shotgun sequence.
ACCESSION   DASUPW010000438 DASUPW010000000
VERSION     DASUPW010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7370
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240782"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VFY44_09875"
     CDS             complement(<1..625)
                     /locus_tag="VFY44_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910791.1"
                     /translation="MSSHPTHRPSDRARPSRGTAAALAIAGLSLFVALDGSAVAAQSM
                     VTARHIATGAVTSAKVKKGAITMKHMSPAARKALRGARGAAGANGAGGANGLSGTNGA
                     NGLTGATGSAGATGGTGGAGATGGQGPKGDTGDPGDPGADGVVTPLSVTAGQVLLLTA
                     TPPTTVVERSVPAGKYIVLGKTQITHSGAGDSVHCELKTGAQVIDQVD"
     gene            complement(734..1588)
                     /locus_tag="VFY44_09880"
     CDS             complement(734..1588)
                     /locus_tag="VFY44_09880"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5910792.1"
                     /translation="MSDAMTIAGHALREAVRRRVLLVVALLTAVFLGLFALAAFEAFD
                     SLEQTGAGGIPLDERALAGGTLLGLSMFVTLFLGVVLAVFLTLDAVRGDAERGLLQPL
                     VVRPVGRTTLLAGRLLAASAVCASYVVVVFLLAVLITHAAGDFWPSSVVVPAAGLALG
                     VVSVVAICLLGSTLLSTTANGIAIFMVFATGLAAGLLGQIGEGLDVDALTTASSVISW
                     IVPFEALYQNGLHALTADIGGAAGVIVQLGPFGGASSVGAAIWPWAVAWSAIVLALAA
                     LAFRRRDL"
     gene            complement(1585..2427)
                     /locus_tag="VFY44_09885"
     CDS             complement(1585..2427)
                     /locus_tag="VFY44_09885"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5910793.1"
                     /translation="MTAALSAHALSKRYGSTAALDAVDLEVGQGELVGLLGPNGAGKS
                     TLVKIACGLVRPTGGRAQVAGAPAGSRAARASTGYLAELFRFPSWAGADEVLSLHQRL
                     ARSDGGPAEREELLARVGLADEGGRRMATMSKGMQQRLGIAQALIGNPRLVLLDEPTS
                     ALDPGGRRTVRDLLVGLREQGVGVLLNSHLLSEIELVCDRVVIIDGGRVVTEGRPQDL
                     TAPGGVEIETSEGVRRLPDARREDVPGIVRELVAGGAEVYGVRVVSGTLEDAYLEAVV
                     GRDR"
     gene            complement(2499..3920)
                     /locus_tag="VFY44_09890"
     CDS             complement(2499..3920)
                     /locus_tag="VFY44_09890"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5910794.1"
                     /translation="MRARAYVPSLLAIGLVVSVISSLGAPLIPSIAGDLGSSLSATQW
                     SLTATLLVGAVASPVVGRLGDGPHRRTVLLVCLAIVTLGGALAALATTLPVLVAGRAM
                     QGLGLALMPLTMASARDHLPAERAGSTIALLSVIGAAGVGLGYPITGFIAEYLDSSAA
                     FWFGAVASGLAFAMAAVFVPSPRDPAHPKSLDVLGAALIGAGLIALLLALEKGADWGW
                     GAPRTAALLALAVVLLALWTRNELRVDEPLVELRLVRRRSVLTANVTGLVLGVAMYLG
                     ISLMTQIVQLDTGIGQTVFVAGLTLVPFAIVSALSSRLLPVVQRRTGRRATIPIGASV
                     IAASMIFFGLTGDALWEAFVTMGVVGLGLGFTFAAMPGLIVGSVPQHETSSAMSFYQV
                     TRYVGFSIGSGLAVTLLRAFDGDSVPDQSAYSATFLVGAALCLLAAVIAWVLPGDALD
                     RVAEGLDEPHIDAVGIEATSRAA"
     gene            3944..5134
                     /locus_tag="VFY44_09895"
     CDS             3944..5134
                     /locus_tag="VFY44_09895"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5910795.1"
                     /translation="MTAPSRTRAFAIVAFAFVVAMAGTTLPTPLYPIYEQRFGFSGVT
                     VTVIFATYAVGVIAALVLFGNLSDRIGRKRVLLPGLLAGALSAVCFLVAEGLPLLLVG
                     RFLSGLSAGIFTGTATATLVDLAPGGDRDRASLVATIANMGGLGLGPPLAGLFAEALP
                     DPIRLVFLTHLVLLGVAALGLRAIAEPTGASGPFAIRPRGFEVPPEVRTAFIRASVAG
                     FAGFAVAGLFTAVAPAFLGQVLGVTNHLVIGLVVMCLFAGSIAGQVLRRRVDDRAALA
                     GGCATLIAGMAILVTSLQVESVALMVLAAVVGGAGQGLSFAAALAMVAGGAPADRRAA
                     VTSSLFVVTYVAISLPVVGVGLLADATDLVEAGTVFAGIVALLAAGAALSLVRSPRAH
                     VSRT"
     gene            complement(5131..5703)
                     /locus_tag="VFY44_09900"
     CDS             complement(5131..5703)
                     /locus_tag="VFY44_09900"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5910796.1"
                     /translation="MSTEPTTRRPRWIEDAERLDLAIYAAVARTPTPALDVVMSRLSR
                     TADYSRLSLLSAAALAVAGGRTGRRAAALGLGSLGVTSTVVNAAVKPLARRRRPDRLA
                     GEVPVARHVAMPTSRSFPSGHSAAAFAFATGVGHVSPGAAAPLRVLAATVAYSRVHTG
                     VHYPGDVLAGALIGTAVAQVTTHMIESRRS"
     gene            5718..7034
                     /locus_tag="VFY44_09905"
     CDS             5718..7034
                     /locus_tag="VFY44_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX5910797.1"
                     /translation="MSRAQAAAAVALVTAAATLALAVAIAVSEFPRGLVVLALVVVAA
                     ASAWYGVLRRGAARTAGIGAAMLALTGIVVLLLAGGSLLAQLLVVAGLVLSLAAARMA
                     LEVRVTLPDAEAPSRPVLFFNPRSGGGKAGRFALAGEARGRGIEPIELTPGQDLTALV
                     QGAIDRGADGLAMAGGDGSQAIVAALAARHGLPYACVPAGTRNHFALDLGVDRDDVVG
                     ALDAFVDGGERTVDLADVNGRVFVNNVSLGLYAEAVQREGYRDAKLRTLLDTVPDVLG
                     PHGGEVDLRWTGPGGRAHHSGAAVLVSNNRYRLGRAVGSGTRPRIDDGLLGMTVFGAP
                     TDRGQGGRRLQRPWREWTAPTFEVDAAGPVAAGVDGEALTLDPPLRFRIRPGVLRVRI
                     SRRHPGASPSAIAPEGLGDGLAALARIARGRIQQPTTPTTRSPEWN"
     gene            7025..7243
                     /locus_tag="VFY44_09910"
     CDS             7025..7243
                     /locus_tag="VFY44_09910"
                     /inference="COORDINATES: protein motif:HMM:NF040111.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amphi-Trp domain-containing protein"
                     /protein_id="HEX5910798.1"
                     /translation="MELMEITEKERMSREEAAKRIHAIADALARNNDVEFDRGGMHFK
                     VRVPDEVEFKFEVEIEDDEREIEIELKW"
BASE COUNT          978 a         2686 c         2783 g          923 t
ORIGIN      
        1 tgtccacctg gtcgatcacc tgcgccccgg tcttgagctc gcagtggacc gagtccccgg
       61 caccggagtg ggtgatctgg gtcttgccga gcacgatgta cttgccggcg gggaccgatc
      121 gctcgacgac ggtcgtgggg ggtgtggccg tgaggagcag cacctggccg gcggtgacgc
      181 tcagcggggt gaccacgccg tcggcgccgg gatcaccggg atcgccggta tcgcccttgg
      241 ggccctggcc gccggtggcg cccgcgccgc cggtgccgcc ggtcgcgccg gcgctaccgg
      301 tggcgccggt gaggccgttc gccccgttgg tgccggagag gccgttggcc ccgccggccc
      361 cgttggctcc ggcggccccg cgagctcccc tgagggcctt gcgcgcagcc ggcgacatgt
      421 gcttcatcgt gatggcgccc ttcttgacct tggccgaggt cacggcaccg gtggcgatgt
      481 ggcgggccgt gaccatgctc tgcgcggcca cggcggagcc gtcgagcgcg acgaacaggg
      541 acaggccggc gatcgccagg gctgcggcgg tgcctcggga tgggcgtgca cggtccgaag
      601 gacggtgcgt gggatggctg gacatcagat tgctcccctg cgtcgtggag gagcagctgg
      661 tgccccggta ctagtcactc gacaccaggg cgctccaccg tgagcgtcct ggaccaatgg
      721 tgtggcgccg gcctcagagg tcgcggcgtc ggaacgccag cgccgcaagc gccagcacga
      781 tcgcggacca cgcgaccgcc cacggccaga tggcggcgcc gacgctgctg gcgccgccga
      841 acgggcccag ctgcacgatc acgccggccg ctccgccgat gtccgcggtc agcgcgtgca
      901 gcccgttctg gtagagcgcc tcgaacggga cgatccacga gatcaccgag cttgccgtgg
      961 tcagcgcgtc cacgtccagg ccctcgccga tctggcccag gagcccggcg gccaggcccg
     1021 tggcgaagac catgaagatc gcgatgccgt tggccgtggt cgacagcagc gtcgagccca
     1081 gcaggcagat cgcgacgacg ctgaccacgc ccagcgccag gccggccgcg ggcacgacga
     1141 ccgacgaggg ccagaagtca ccggcggcgt gcgtgatgag cacggccagg aggaagacga
     1201 cgaccacgta ggaggcgcag acggcggagg cggccagcag ccgcccggcc agcagcgtcg
     1261 tgcggccgac cgggcgcacc accagcggtt gcagcaggcc gcgctcggca tcgccgcgga
     1321 cggcgtccag cgtgaggaac acggccagca ccacgccgag gaagagcgtg acgaacatcg
     1381 acagccccag gagcgtgccg cccgccagcg cgcgctcgtc cagcgggatg cccccggcgc
     1441 cggtctgctc gagcgagtcg aaggcctcga aggcggccag cgcgaacagg ccgaggaaga
     1501 cggcggtcag cagcgcgacc acgagcagga cgcggcgccg caccgcctcc cgcagcgcgt
     1561 ggccggcgat ggtcatcgcg tcgctcaccg gtcgcgcccc acgacggcct cgaggtaggc
     1621 gtcctcgagc gtcccgctga ccacgcggac gccgtagacc tcggccccgc ccgccacgag
     1681 ctcgcggaca attccgggca cgtcctcgcg gcgcgcgtcg ggcaggcggc ggacgccctc
     1741 ggaggtctcg atctcgacgc cgccgggcgc ggtcaggtcc tgcggccgcc cttcggtgac
     1801 cacccggccg ccgtcgatga tcacgacccg gtcgcacacc agctcgatct cgctgagcag
     1861 gtgtgagttc agcagcacgc cgacaccctg ctcgcgcagg cccacgagca ggtcacggac
     1921 cgtgcgcctg ccgcccgggt cgagcgcgct ggtcggctcg tccagcagca cgagccgcgg
     1981 gttcccgatc agcgcctgcg cgatgcccag gcgctgctgc atgcccttgc tcatcgtggc
     2041 catgcgtcgc ccgccctcgt ctgccagacc gacgcgcgcc agcagctcct cgcgctcggc
     2101 cgggccgccg tccgagcggg ccaggcgctg gtgcagcgac agcacctcgt cggcccccgc
     2161 ccagctggga aagcggaaga gctcggccag gtagccggtc gacgcccgcg ccgcccggct
     2221 cccggcaggg gctccggcca cctgcgcccg gccgccggtg ggccggacca ggccgcaggc
     2281 gatcttgacc agcgtcgact tgccggcccc gttggggccg agcaggccga ccagctcgcc
     2341 ctggccgacc tcgaggtcga cggcgtccag cgcggccgtg ctgccgtagc gcttcgagag
     2401 cgcgtgggct gacagtgctg cggtcacggc cgcggactgt acggcgcgcg ggtcggggcg
     2461 gggaggagga cgcgtgcagg ctcggtgcag ggccggcttc acgctgcgcg cgaagtggcc
     2521 tcgatcccga ccgcgtcgat gtgcggctcg tcgaggccct cggcgacgcg gtcaagggcg
     2581 tcgcccggca gcacccaggc gatcacggcg gccagcaggc acagcgcggc gcccaccagg
     2641 aaggtcgccg agtaggcgct ctggtcgggc acgctgtcgc cgtcgaaggc gcgcagcagc
     2701 gtgaccgcca ggccgctgcc gatcgagaag cccacgtacc gggtcacctg gtagaagctc
     2761 atcgcgctgc tggtctcgtg ctggggcacc gagcccacga tcaacccggg catggccgcg
     2821 aaggtgaagc ccagccccag gcccacgacg cccatggtca cgaacgcctc ccagagcgcg
     2881 tcgcccgtga gcccgaagaa gatcatcgag gccgcgatga ccgaggcgcc gatcgggatc
     2941 gtggcgcgcc ggcccgtgcg ccgctggacc accggcagca accggctgga cagcgccgac
     3001 acgatcgcga acggcaccag tgtcaggccg gccacgaaga ccgtctggcc gattccggtg
     3061 tccagctgga cgatctgcgt catcagcgag atgcccaggt acatggccac gcccagcacc
     3121 aggccggtca cgttcgcggt gagcaccgac cgccgcctga cgagccgtag ctcgaccagg
     3181 ggctcgtcca cgcgcagctc gttgcgggtc cacagggcca gcaacaccac ggccagcgcc
     3241 agcagcgcgg cggtgcgcgg tgcgccccag ccccagtcgg cgcccttctc cagcgccagc
     3301 agcagcgcga tcaggccggc gccgatcagc gccgcgccga gcacgtccag cgacttcggg
     3361 tgcgcggggt cgcgcgggga cggcacgaac accgccgcca tcgcgaacgc gagcccgctg
     3421 gccacggccc cgaaccagaa cgcggccgag gagtccaggt actcggcgat gaagcccgtg
     3481 atcgggtaac ccagcccgac gcccgcggcg ccgatgaccg acagcagcgc gatcgtggac
     3541 ccggcccgtt cggccggcag gtggtcgcgg gccgaggcca tggtcagcgg catcagcgcc
     3601 aggcccaggc cctgcatcgc gcggccggcc accagcacgg gcagggtggt ggccagcgcg
     3661 gccagcgcgc cgccgagcgt gacgatggcc aggcacacca gcagcaccgt gcgccggtgc
     3721 ggcccgtcgc ccagccggcc caccacgggc gaggccaccg cgcccaccag cagcgtcgcg
     3781 gtcagcgacc actgggtggc gctgaggctg gagcccaggt ccccggcgat cgaggggatc
     3841 agcggggcgc cgagcgagct gatgaccgag accaccagcc cgatcgccag caggctcggg
     3901 acgtaggcac gagcacgcac cgcgccaaaa ctagtctcag ggggtgaccg cgcccagccg
     3961 cacccgcgcc ttcgccatcg tggccttcgc cttcgtcgtg gccatggcgg gcacgaccct
     4021 gcccacgccg ctgtatccga tctacgagca gcggttcggg ttcagcgggg tgacggtcac
     4081 ggtgatcttc gccacgtacg cggtgggggt gatcgcggcg ctggtgttgt tcggcaacct
     4141 gtcggaccgg atcgggcgca agcgcgtcct gctgccgggc ctgctggcgg gcgccctgag
     4201 cgccgtctgc ttcctggtgg ccgaggggct gccgctgctg ctggtggggc gcttcctgtc
     4261 cggcctcagc gccgggatct tcaccggcac ggccaccgcg acgctggtgg acctggcgcc
     4321 gggcggcgac cgcgaccgcg cctcgctggt ggccacgatc gcgaacatgg gcggcctggg
     4381 actggggccg ccactggccg ggttgttcgc cgaggcgctg cccgacccga tccgcctggt
     4441 gttccttacc cacctggtgc tgctgggcgt ggccgcgctc gggctccggg ccatcgccga
     4501 gcccaccggc gcctccggcc ccttcgccat ccgcccgcgc gggttcgagg ttccgcccga
     4561 ggtgcgcacg gcgttcatcc gcgccagcgt ggccggcttc gcgggcttcg ccgtcgcggg
     4621 actgttcacc gccgtggccc cggccttcct gggccaggtc ctgggcgtga cgaaccacct
     4681 ggtgatcggg ctggtggtca tgtgcctgtt cgccggctcg atcgcgggcc aggtgctgcg
     4741 ccggcgggtc gacgaccgcg ccgccctggc cggcggctgc gccacgctga tcgccgggat
     4801 ggcgatcctg gtgaccagcc tccaggtgga gtcggtggcg ctgatggtgc tcgcggccgt
     4861 ggtcggcggc gccgggcagg ggctgagctt cgccgccgcc ctggccatgg tcgccggcgg
     4921 cgcgcccgcc gaccgccgcg ccgcggtgac ctcgagcctg ttcgtggtca cctacgtggc
     4981 gatctcgctg ccggtggtgg gcgtcggcct gctggccgac gccaccgacc tggtcgaggc
     5041 cggcacggtg ttcgccggca tcgtcgccct gctcgctgcc ggggcggcgc tgtcgctcgt
     5101 gaggagcccg cgcgcccacg tcagccggac ctaggatcgg cgagactcga tcatgtgagt
     5161 ggtgacctgg gcgacagccg tgccgatcag cgcaccggcc aggacgtcgc cggggtagtg
     5221 cacgccggtg tgcacgcgcg agtacgcgac ggtcgcggcc agcacgcgca ggggcgcggc
     5281 cgcgcccggg gacacgtggc cgacgccggt ggcgaacgcg aacgccgcgg cggagtggcc
     5341 ggagggaaac gagcgcgacg tgggcatggc cacgtggcgc gcgaccggga cctcgccggc
     5401 caggcggtcg ggccgccgcc gccgtgccag cggcttgacg gccgcgttga ccaccgtcga
     5461 ggtgaccccc agcgagccca ggcccagcgc tgctgcgcgc cggccggtgc gcccgccggc
     5521 cacggccagc gccgcggccg acagcaggga caggcgcgag tagtccgccg tacgcgagag
     5581 ccggctcatg accacgtcca gggcgggcgt gggagtgcgc gcgacggcgg cgtagatggc
     5641 caggtccagg cgctcggcat cctcgatcca gcggggccgg cgggtggtcg gctcggtgct
     5701 catgtgggcg ggatcgtatg agcagggctc aggcggcggc ggccgtggcg ctcgtgaccg
     5761 cagcggcgac gctggccctg gccgtggcga tcgcggtcag cgagtttccc cgcggcctcg
     5821 tggtgctggc gctggtcgtg gtggccgcgg cctcggcctg gtacggcgtg ctgcggcgcg
     5881 gcgccgcccg cacggccggg atcggcgcgg cgatgctggc cctgaccggc atcgtcgtgt
     5941 tgctgctggc gggcggatcc ctgctcgcac agctgctggt cgtggccggg ctggtgctgt
     6001 cgctcgcggc ggcccggatg gcgctcgagg ttcgcgtgac gctgcctgat gccgaggccc
     6061 cgagccgccc ggtgttgttc ttcaacccgc gctcgggcgg gggcaaggcc gggcggttcg
     6121 cgctggccgg cgaagcccgt ggccgcggca tcgagcccat cgagctgacc cccgggcagg
     6181 atctgacggc gctggtgcag ggggccatcg atcgcggcgc cgacgggctg gcgatggccg
     6241 gtggcgatgg ctcacaggcc atcgtcgcgg ccctggcggc ccggcacggc ctgccctacg
     6301 cgtgcgtgcc tgccgggacg cgcaaccact tcgcgctcga cctcggcgtg gaccgtgacg
     6361 atgtggtcgg cgctctggac gcgttcgtgg acgggggtga gcggacggtc gacctggcgg
     6421 acgtcaacgg gcgcgtgttc gtgaacaacg tctcgctcgg gctgtacgcc gaggcggtcc
     6481 agcgggaggg ctaccgcgac gcgaagttgc gcacgctgct ggacacggtg ccggacgtgc
     6541 tcggacctca tggcggtgag gtggacctgc gctggaccgg cccgggaggg cgcgcccacc
     6601 actcgggcgc cgccgtgctg gtgtccaaca accgctaccg cctgggtcgc gcggtcggct
     6661 cgggcacccg cccgcggatc gacgacggcc tcctggggat gacggtcttc ggcgcgccga
     6721 ccgatcgggg ccagggtggc cgccggctgc agcgcccgtg gcgcgaatgg acggcgccca
     6781 ccttcgaggt ggacgccgcc ggccccgtcg cggccggggt ggacggcgag gcgctgaccc
     6841 tcgatccgcc gctgcgcttc cgcatccgcc ccggcgtgct gcgcgtccgc atctcgcgcc
     6901 gccaccccgg cgcctcccct tcggccatcg cccccgaggg cctcggggac ggtctggccg
     6961 cactggcccg gatcgcccgc ggccggatcc agcaacccac cacacccacg acgaggagcc
     7021 ccgaatggaa ctgatggaga tcaccgagaa ggaacgcatg agccgcgagg aggccgccaa
     7081 gcgcatccac gcgatcgcgg acgcgctggc ccggaacaac gacgtcgagt tcgaccgcgg
     7141 cggcatgcac ttcaaggtgc gcgtgcccga cgaggtcgag ttcaagttcg aggtcgagat
     7201 cgaggacgac gagagggaga tcgagatcga gctcaagtgg tgacccgcta ggaggcggcc
     7261 acgggcgtgt agcgcaggta ggtcacgccg ttctcgtacg agtcggacgc cgccagcgag
     7321 aacttgccgg gagcggcccc gtccgggaac agccgcagcc cggtgcccag
//
LOCUS       DASUPW010000439         4100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1625332, whole genome shotgun sequence.
ACCESSION   DASUPW010000439 DASUPW010000000
VERSION     DASUPW010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625332"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1686
                     /gene="thrS"
                     /locus_tag="VFY44_09915"
     CDS             <1..1686
                     /gene="thrS"
                     /locus_tag="VFY44_09915"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX5910799.1"
                     /translation="LAAAVLDLYPGTKISIGPPIEDGFYYDFEFPEDVKLSDADFERI
                     EAEMRKHVKADEQFTREDVPVAEALERFRAEGQDYKVELIEDLVRDEGVETVSLYTNG
                     PFTDLCRGPHAPSTKRIKAFKLTSVAGAYWRGDADRQMLTRVYGTAFLSKDDLAAHLE
                     RLEEARKRDHRKLGRELDLFFLSEISPGSPFWKPDGARIWAQLSDFWRRENVGRGYDE
                     VRTPILYDVDLWKQSGHWDTYRDNMYFTEVENRQMGLKPMNCPAHIQIYKSDRRSYRD
                     LPIRYAEAGLVHRHEPSGTLHGLLRVRHITQDDGHIFCTEDQVKDEVKACLAFGFFIY
                     DVFGFEPRLELSTRPEKRVGSDEMWDRAEASLEAALVEEGLDFEVNEGDGAFYGPKID
                     LHMTDSIGRSWQLGTVQLDYSMPERFELAYTGADNEDHRPVMIHRALMGSFERFIGIL
                     IEHYAGEFPLWLAPAQALVLPISDRHVEYAEKVARELRDQGLRVAVDDRGESVGRKIR
                     DGELRKVPYLLVVGDQEADSGQVAVRRHGEGDLGAEAVGAFAARVEAQVRDRT"
     gene            1812..2420
                     /gene="infC"
                     /locus_tag="VFY44_09920"
     CDS             1812..2420
                     /gene="infC"
                     /locus_tag="VFY44_09920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX5910800.1"
                     /translation="MAIPRRFDRRPPDRDDTRINERIRIREVRLIGDDGNQIGVVPTE
                     KALAYARERDLDLVEVAPDARPPVCRVLDYSKYKYEQEQKAKAAKKHQKQVNVREIKL
                     RPKIADNDYETKKNHVVRFLKGDDRVKVTIMFRGREQTHPERGEALLLRLAEDVAELG
                     TVEQRPIQDGRNMTMLLSPVPHKTEADEPAAAEPDAPAAEPA"
     gene            2429..3079
                     /locus_tag="VFY44_09925"
     CDS             2429..3079
                     /locus_tag="VFY44_09925"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5910801.1"
                     /translation="MRRLAHAAAKRVGAVRLRRLIAGTRPPYRLELGTNEVNHDGWIP
                     TDVQWRARNYLDATGPWPFPEDSISHVYADNVIEHVSLDGARSLLREAARTMQPGGRI
                     RLVTPDARRCAEVYLEGGERAEAQKDAHRGAGTRVEHDVDILRGVFIEYEHRYGYAWD
                     QEALTAELEAAGFTGVERCELGESDDPALGGLEWRVLPIDRETMLVLEARAPGLLQ"
     gene            3089..3280
                     /gene="rpmI"
                     /locus_tag="VFY44_09930"
     CDS             3089..3280
                     /gene="rpmI"
                     /locus_tag="VFY44_09930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX5910802.1"
                     /translation="MPKMKSHSGAKKRFRKTGTGKIRGRHGMTSHILEKKSARRKRKL
                     GRPHIIDKADVPRVKELLK"
     gene            3280..3639
                     /gene="rplT"
                     /locus_tag="VFY44_09935"
     CDS             3280..3639
                     /gene="rplT"
                     /locus_tag="VFY44_09935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX5910803.1"
                     /translation="MARVKRSVHARKKRRSTLERAKGFRGQRKSSYKQAKETLLKADT
                     YAYRDRRNKKRDFRRLWITRINAAARQEGLTYGTFMHGLKVAGVELDRKVLADIAVND
                     KETFRRFAETAREAAAA"
     gene            3713..>4100
                     /locus_tag="VFY44_09940"
     CDS             3713..>4100
                     /locus_tag="VFY44_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910804.1"
                     /translation="MITSSDNSQLKLIRKLSRKKARDEAGLFVAEGDDLVAAAVAAGW
                     EPEVLLWSGQDVEPELLDRVSQLGSGTRVIGVYPQRWAEPGGDLSVALHGVGDPGNVG
                     TIIRSAHALADGPVLIAPGTADPYGPK"
BASE COUNT          702 a         1420 c         1449 g          529 t
ORIGIN      
        1 ctcgccgccg cggtgctgga cctctatccc ggcaccaaga tctcgatcgg cccgccgatc
       61 gaggacggct tctactacga cttcgagttc cccgaggacg tcaagctcag cgacgcggac
      121 ttcgagcgga tcgaggccga gatgcgcaag cacgtcaagg ccgacgagca gttcacgcgc
      181 gaggacgtcc cggtggccga ggccctggag cggttccggg cggaggggca ggactacaag
      241 gtcgagctga tcgaggacct ggtccgcgac gagggcgtgg agacggtgtc gctgtacacc
      301 aacggcccct tcaccgacct gtgccgcggc ccgcacgccc cgtcgaccaa gcggatcaag
      361 gccttcaagc tgaccagcgt ggccggcgcc tactggcgcg gggacgccga tcgccagatg
      421 ctcacgcgcg tgtacggcac ggcgttcctg tccaaggacg acctggcggc acacctggaa
      481 cgcctggagg aggcgcgcaa gcgcgaccac cgcaagctgg gccgcgagct ggacctgttc
      541 ttcctgtccg agatctcgcc ggggtcgccg ttctggaagc ccgacggcgc ccgcatctgg
      601 gcccagctga gcgacttctg gcgccgggag aacgtgggcc gcggctacga cgaggtgcgc
      661 acgccgatcc tctacgacgt ggacctgtgg aagcagtcgg gccactggga cacctaccgc
      721 gacaacatgt acttcaccga ggtggagaac cggcagatgg ggctcaagcc catgaactgc
      781 ccggcccaca tccagatcta caagtccgac cggcgctcct accgcgacct gccgatccgt
      841 tacgcggagg ccggcctggt gcaccgccac gagcccagcg gcacgctcca cgggctgctg
      901 cgcgtgcggc acatcaccca ggacgacggc cacatcttct gcaccgagga ccaggtcaag
      961 gacgaggtca aggcctgcct ggccttcggc ttcttcatct acgacgtgtt cgggttcgag
     1021 ccgcgcctgg agctgtccac ccgacccgag aagcgggtcg gctcggacga gatgtgggac
     1081 cgcgccgagg cctcgctgga ggccgccctg gtcgaggagg ggctcgactt cgaggtcaac
     1141 gagggcgacg gcgccttcta cgggcccaag atcgacctgc acatgaccga ctcgatcggc
     1201 cgttcgtggc agctgggcac ggtccagctg gactactcga tgcccgagcg gttcgagctg
     1261 gcctacaccg gcgccgacaa cgaggaccac cgcccggtga tgatccaccg ggcgctgatg
     1321 ggcagcttcg agcgcttcat cgggatcctg atcgagcact acgccggcga gttcccgctg
     1381 tggctggcgc cggcgcaggc gctggtgctg cccatctccg atcgccacgt ggagtacgcg
     1441 gagaaggtgg cgcgcgagct cagggaccag ggcctgcgcg tggcggtcga cgaccgcggc
     1501 gagtccgtcg ggcgcaagat ccgcgacggg gagctgcgca aggtgccgta cctgctggtg
     1561 gtgggcgacc aggaggcgga ctcgggccag gtggcggtgc ggcgccacgg cgaaggcgac
     1621 ctgggggccg aggcggtggg cgcgttcgcc gcacgggtcg aggcacaggt tcgcgaccgc
     1681 acctgacgag cagcgctata gtcaccttcg aatgctgggc aaccacacca cgcgctgcga
     1741 ccgctaacgc gcccttgccc tctcccgact cacctttcct tcttgccttc tagtcccatc
     1801 cgcttcagct agtggcgatt ccgcgcaggt tcgatcggcg accgcccgac cgggacgaca
     1861 cccgcatcaa cgagcggatc cgcatccgcg aggtgcggct gatcggcgac gacggcaacc
     1921 agatcggcgt cgtgccgacc gagaaggccc tggcctacgc ccgcgagcgt gacctggacc
     1981 tggtcgaggt cgcccccgac gcccggccgc cggtgtgccg cgtcctggac tactcgaagt
     2041 acaagtacga gcaggagcag aaggccaagg ccgccaagaa gcaccagaag caggtcaacg
     2101 tgcgcgagat caagctgcgc cccaagatcg cggacaacga ctacgagacc aagaagaacc
     2161 acgtggtccg gttcctcaag ggcgacgacc gcgtgaaggt cacgatcatg ttccgcggcc
     2221 gcgagcagac ccaccccgag cgcggggagg ccctgctgct gcgcctggcc gaggacgtgg
     2281 ccgagcttgg cacggtcgag cagcggccga tccaggacgg gcgcaacatg accatgctgc
     2341 tctcgccggt gccgcacaag accgaggccg acgagcccgc ggccgccgag cccgacgcgc
     2401 ccgcggccga gcccgcctga gacccgcggt gcggcgcctg gcgcacgcag ccgccaagcg
     2461 ggtgggcgcg gtccggctac gccgcctgat cgccggcacc aggccgccct accggctgga
     2521 gctgggcacc aacgaggtca accacgacgg gtggatcccc accgacgtcc agtggcgcgc
     2581 ccgcaactac ctggacgcca cgggtccgtg gccgttcccg gaggactcga tcagccacgt
     2641 gtacgccgac aacgtgatcg agcacgtgtc gctcgacggc gcccgctcgc tgctgcgcga
     2701 ggcggcccgg acgatgcagc cgggcggccg gatccgcctg gtcacgcccg acgccaggcg
     2761 ctgtgccgag gtctacctgg agggcggcga gcgcgccgag gcccagaagg acgcccaccg
     2821 gggcgcgggc acgcgcgtgg agcacgacgt ggacatcctg cgcggggtgt tcatcgagta
     2881 cgagcaccgg tacggctacg cctgggacca ggaggcgctg accgcggagc tcgaggccgc
     2941 gggattcacg ggcgtggagc ggtgcgagct gggggagagc gacgatcccg cgctcggcgg
     3001 cctggagtgg cgcgtgctgc cgatcgaccg ggagacgatg ctcgtcctgg aggctcgggc
     3061 gcccggtctg cttcaatagg ccgtcgccat gccgaagatg aagtctcatt cgggcgccaa
     3121 gaagcgcttt cgcaagaccg gaaccgggaa gatccggggc cgtcacggca tgaccagcca
     3181 catcctggag aagaagagcg cccggcgtaa gcgcaagctc ggccggccgc acatcatcga
     3241 caaggccgac gtgccgcggg tcaaggagct cctgaagtaa tggcccgcgt caagcgctcc
     3301 gtccacgcgc gcaagaagcg ccgcagcacg ctcgagcggg ccaaggggtt ccgtggccag
     3361 cgcaagtcca gctacaagca ggcgaaggag acgctgctca aggcggacac ctacgcctac
     3421 cgcgaccggc gcaacaagaa gcgcgacttc cgccggctgt ggatcacccg catcaacgcc
     3481 gccgcccgcc aggagggcct gacctacggc accttcatgc acggcctcaa ggtcgcgggc
     3541 gtggagctgg accgcaaggt cctggccgac atcgccgtga acgacaagga gaccttccga
     3601 cgttttgccg agaccgcccg ggaggcggcc gctgcctagt tcggcagacg gccaccgcag
     3661 caccccgggc gcccagcgag gcgccttttt tgtgccctga ggagaccaga ccgtgatcac
     3721 atcgtccgac aacagccagc tgaagctcat ccgcaagctc tcgcgcaaga aggcgcgcga
     3781 cgaggcgggc ctgttcgtgg ccgagggcga cgacctggtc gcggccgcgg tggccgcggg
     3841 ctgggagccg gaggtgctgc tgtggtcggg ccaggacgtg gagcccgagc tgctggaccg
     3901 ggtctcccag ctgggatccg gcacgcgcgt gatcggcgtc tacccccagc gctgggcgga
     3961 gcccggcggg gacctgtcgg tggcgctgca cggggtgggg gacccgggca acgtggggac
     4021 gatcatccgc tccgcccacg cgctggccga cggaccggtg ctgatcgccc ccggcacggc
     4081 cgacccgtac gggcccaagg
//
LOCUS       DASUPW010000440         2242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1625496, whole genome shotgun sequence.
ACCESSION   DASUPW010000440 DASUPW010000000
VERSION     DASUPW010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625496"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2004)
                     /locus_tag="VFY44_09945"
     CDS             complement(<1..2004)
                     /locus_tag="VFY44_09945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5910805.1"
                     /translation="MHILVMTDRDWTHPQGGGTGANLYGQVSRWLSWGHRVSIVAAAY
                     EGCQAHEQLGALEIHRVGGRSTVFPRVIWRGMRGRLPEADVVLEVVNGITFLSPLWLR
                     TPSVTLVHHVHRSHYVEEMGRKGALAALLLETLPLKVLYARSRFSTISNATADEMVEQ
                     LGLERDRIEVNYIGVELDTLAPGVRAEEPTLLFLGRLKRYKRIEILLDVLEQIPEAVL
                     EVAGDGDHGPALEEEIARRGLSDRVRMHGHVNEATKLELLQRAWVNLTASSAEGWCLT
                     VMEAAGCRTPSAALAVGGLPESIDGDRTGLLAHTPAELGEQVGRLVHDHDLRERLGSQ
                     ALERARTFTWDAAAERSLDSLEAARAEGPQPSFLKSLSRSDTGRAAGLASAVIATNVV
                     ALLSAFIILMVPGSALQIAVAREISTQIAAGDPHPGRGVRRWLSRLALATCLVAAAAI
                     PLREPLAAAINVDQTWAAASVPVSSMAWMLVCVERGALQGFQRYREVGLSLVGEATLR
                     TVIALLLVAAGLGVTGAFLGTGLALGLLALILFAILRRQLPPADVSVAGASRLRSLLA
                     GAWAPVLGMTLLFALQEVHVIVVKHEATAAQASSWAVAAVAAKGIIWVAVGLGMYLLP
                     EVARRAAEGEDARPILARTLGLIAALAIPMVLVYAVAAQPLLSA"
     gene            2052..>2242
                     /locus_tag="VFY44_09950"
     CDS             2052..>2242
                     /locus_tag="VFY44_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910806.1"
                     /translation="MSIDPAAIARDTGALVAVPSVTGDERAALELLGRQAEALGLDAR
                     LHTHDLERLRSLPGHPGQEA"
BASE COUNT          311 a          839 c          836 g          256 t
ORIGIN      
        1 cgccgacagc agcggctggg cggccaccgc gtacaccagg accatcggga tcgccagggc
       61 ggcgatcagg cccagcgtgc gcgccaggat cggccgcgcg tcctcgccct cggcggcccg
      121 gcgggccacc tccgggagca ggtacatgcc caggccgacc gccacccaga tgatgccctt
      181 ggccgcgacc gccgccacgg cccacgagct ggcctgcgcg gcggtggcct cgtgcttgac
      241 cacgatcacg tggacctcct ggagggcgaa cagcagggtc atgcccagca cgggcgccca
      301 cgcccccgcc agcagcgagc gcagccgcga cgcgccggcc acgctgacgt ccgcaggcgg
      361 cagctgacgg cgcaggatgg cgaacaggat cagcgccagc aggcccagcg ccaggccggt
      421 gcccaggaac gcgccggtca cgcccaggcc ggcggccacc agcagcagcg cgatcaccgt
      481 ccgcagcgtc gcctcgccga ccaggctcag gcccacctcg cggtagcgct ggaagccctg
      541 gagcgcgccg cgctccacgc acaccagcat ccaggccatg ctggacacgg gcacggaggc
      601 cgcggcccac gtctggtcca cgttgatggc ggccgcgagc ggctcgcgca gcgggatggc
      661 ggcggcggcc accaggcagg tggccagcgc caggcggctg agccatcggc gcacgccgcg
      721 acccgggtgc gggtcgccgg ccgcgatctg ggtgctgatc tcgcgcgcga ccgcgatctg
      781 gagcgcggag ccggggacca tgaggatgat gaaggccgac agcagcgcga ccacgttggt
      841 ggcgatgacc gcggaggcca ggccggccgc gcggcccgtg tccgagcgcg acagcgactt
      901 caggaagctg ggctgggggc cctcggcgcg ggcggcctcg agcgagtcca gcgagcgttc
      961 cgcggcggcg tcccaggtga aggtgcgggc gcgctccagg gcctgggagc cgagccgctc
     1021 gcgcaggtcg tggtcgtgca ccaggcggcc gacctgctcg ccgagctcgg cgggcgtgtg
     1081 cgccaggagg ccggtgcggt cgccgtcgat cgactccggc agcccgccca cggccagcgc
     1141 ggcgctgggc gtgcggcagc cggcggcctc catgaccgtc aggcaccagc cctcggcgga
     1201 ggacgcggtc aggttgaccc acgcgcgctg gagcagctcc agcttggtcg cctcgttcac
     1261 gtgaccgtgc atgcgcacgc ggtcgctcag cccgcggcgc gcgatctcct cctccagcgc
     1321 cggcccgtgg tcgccgtcgc cggccacctc cagcaccgcc tccgggatct gctccaggac
     1381 gtccagcagg atctcgatcc gcttgtagcg cttgagcctg cccaggaaca gcagcgtggg
     1441 ctcctccgcg cggacgccgg gggcgagcgt gtccagctcg acgccgatgt agttgacctc
     1501 gatccggtcc cgctccagcc cgagctgctc caccatctcg tcggcggtgg cgttggagat
     1561 ggtcgagaag cgcgagcggg cgtacagcac cttcagcggc agggtctcga gcagcagcgc
     1621 ggccagcgcg cccttgcggc ccatctcctc cacgtagtgg ctgcggtgca cgtggtgcac
     1681 cagcgtcacc gacggggtgc ggagccagag gggcgagagg aaggtgatgc cgttgaccac
     1741 ctcgagcacc acgtcggcct ccggcagccg cccgcgcatg ccgcgccaga tcacgcgcgg
     1801 gaacacggtc gagcgcccgc ccacgcggtg gatctccagc gctcccagct gctcgtgcgc
     1861 ctggcacccc tcgtaggccg cggcgacgat ggacacgcgg tgcccccacg agagccagcg
     1921 cgagacctgg ccgtacaggt tggctccggt cccgcccccc tgcgggtggg tccagtcccg
     1981 atcggtcatg acgaggatgt gcacccgccg caggctaccg gcggccggtt gcgggtaccg
     2041 tgcgccggga ggtgagcatc gaccccgcag ccatcgcccg cgacaccggt gcgctggtgg
     2101 cagtgccgag cgtgacgggc gacgagcgcg cggcgctgga gctgctgggg cggcaggccg
     2161 aggccctggg cctggacgcg cggctgcaca cccacgacct cgagcgcctg cggtcgctgc
     2221 cggggcatcc cgggcaggag gc
//
LOCUS       DASUPW010000441         2051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_794638, whole genome shotgun sequence.
ACCESSION   DASUPW010000441 DASUPW010000000
VERSION     DASUPW010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794638"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1655
                     /locus_tag="VFY44_09955"
     CDS             <1..1655
                     /locus_tag="VFY44_09955"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5910807.1"
                     /translation="DERVALKVFRGGEMDGASARAHFKRTVQAQAALSHPNVVAIHDW
                     GTEPAPYLAMTMVQGTTLAEMLANGRRRGLPALEILGAVARGLDAAREQGLVYCRLHP
                     SGVLVAEDGTTMLGDFGAGRSARAVELLGRGSLGAYADYIAPEAIEGGGATPAGNVYA
                     LGAMAFHVLAGHPPFVSEYEGVTLQAHLDAPPKGPGSERPGIPTRLDQAVVRALSKDP
                     ALRQGSAGELMDTVIEAYAEAGLASEPAADAVPARKRRARRPRLVPALLAVAGVIAAA
                     ALGAAAAGGEDPPRAPEPARAAGTKDVRVSHPASWRVARQAPEIAGLRLADPVAVSPP
                     GGPIELVAGRVDGAVPGRPGQLVDLGGVIGRRHSGLRETGSRRPVVAYSVPGAEGTAV
                     AACVGSGSRAARAACERVASTVRPRREGVGLAAASPAYARAVSAVVRRIGAVRIKGRR
                     RLARARTPAGQRRITARLARDYAVLARAMARVEAPAPARASQRVIVSQLRRAARGYRD
                     MSRAARRRSGRRWVAARAGVRSAEGRVQSALRSLRRQGYRVR"
     gene            complement(1660..>2051)
                     /locus_tag="VFY44_09960"
     CDS             complement(1660..>2051)
                     /locus_tag="VFY44_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910808.1"
                     /translation="ISSNPLITRPLDGSAERPHALEIYMDSMRQTRQMDLDIVLPGHG
                     EPFTDHAQLIDKRFALHQRRAQKLYALIAERPRTAYELAQALWGNIAVTQAYLTLSEV
                     LGHTDLLQTAGRVGEVEADGVVRFEAA"
BASE COUNT          237 a          732 c          835 g          247 t
ORIGIN      
        1 ccgacgagcg ggtggcgctc aaggtcttcc gaggcggcga gatggacggc gcgtcggccc
       61 gggcccactt caagcgcacg gtccaggcgc aggccgcgct gtcgcatccg aacgtggtgg
      121 ccatccacga ctggggaacc gaaccggctc cgtacctggc gatgacgatg gtccagggga
      181 ccaccctggc ggagatgctg gccaacggcc ggcgccgcgg gctaccggcg ctcgagatcc
      241 tcggcgcggt cgcgaggggc ctggacgcgg cacgcgagca ggggctcgtg tactgccgcc
      301 tgcacccgtc gggcgtgctg gtggccgagg acgggaccac gatgctcggc gacttcggcg
      361 ccgggcgcag cgcgcgcgcg gtcgagctgc tcgggcgtgg cagcctgggc gcgtacgccg
      421 actacatcgc gccggaggcc atcgagggcg gtggcgccac ccccgcgggc aacgtctacg
      481 ccctcggagc catggccttc cacgtgctgg cgggccaccc gccgttcgtc tcggagtacg
      541 agggtgtgac gctccaggcc cacctggacg cgccgccgaa gggccccggc agcgaacggc
      601 cgggcatccc cacgcgcctg gaccaggccg tggtccgcgc gctctccaag gacccggcgc
      661 tgcgtcaggg gtcggcgggc gagctgatgg acacggtgat cgaggcctac gccgaggcgg
      721 ggctcgcgtc ggagcccgcg gccgacgccg tgccggcgcg gaagcgacgt gcccgtcgcc
      781 cgcggctggt cccggccctg ctggcggtgg cgggcgtgat cgccgcggcg gccctgggcg
      841 cggcggccgc cgggggagag gatccgccgc gcgcgcccga gccggcgcga gccgccggaa
      901 cgaaggacgt gcgcgtgagc caccccgcga gctggcgtgt ggcgcgccag gcaccggaga
      961 tcgccgggct gcgcctggcg gacccggtcg ccgtctcgcc gcccggcggg ccgatcgagc
     1021 tggtggccgg acgcgtcgac ggagcggtgc ccggccgccc cggtcagctg gtggacctcg
     1081 gaggcgtgat cgggcgccgg cactcggggc tgcgggagac ggggtcgcgg cgtcctgtcg
     1141 tcgcctactc cgtgccgggc gccgagggca ccgcggtggc cgcctgcgtg ggctcgggat
     1201 cccgtgcggc ccgggccgcg tgcgagcgcg tggcgtcgac ggtcaggccg cgccgcgagg
     1261 gcgtggggct cgccgccgcc agcccggcct acgcccgggc ggtgagcgcc gtcgtgcgcc
     1321 gcatcggagc ggtccggatc aagggacgcc gcaggctggc cagggcccgc acccccgcgg
     1381 gccagcgacg cataaccgct cgcctggcgc gcgactacgc ggtgcttgcg cgcgccatgg
     1441 cgcgcgtcga ggctccggcg ccggcaaggg cgtcgcagcg cgtgatcgtg agccagctac
     1501 ggagggcggc gcgcgggtac cgggacatgt cgcgggccgc gcgcaggcgc tcgggccggc
     1561 ggtgggtggc ggcacgggcc ggtgtccggt cggccgaggg acgcgtccag agcgcgctgc
     1621 gcagccttcg ccgccagggc taccgggtcc gctgagcgct cacgccgcct cgaaccgcac
     1681 cacgccgtcc gcctcgacct cgcccacgcg gccggcggtc tgaagcaggt cggtgtgccc
     1741 cagcacctcg ctcagcgtca ggtaggcctg ggtcacggcg atgttgcccc acagggcctg
     1801 ggccagctcg taggcggtgc ggggacgctc cgcgataagc gcgtacagct tctgggccct
     1861 gcgctggtgc agggcgaagc gcttgtcgat cagctgcgcg tggtcggtga acggctcgcc
     1921 gtggccgggg agcacgatgt ccaggtccat ctgccgggtc tgacgcatcg agtccatgta
     1981 gatctcgagc gcgtggggac gctcggccga gccgtccagc gggcgggtga tcagcgggtt
     2041 ggacgagatg t
//
LOCUS       DASUPW010000442         8829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1764527, whole genome shotgun sequence.
ACCESSION   DASUPW010000442 DASUPW010000000
VERSION     DASUPW010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8829
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1764527"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="VFY44_09965"
     CDS             complement(<1..995)
                     /locus_tag="VFY44_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910809.1"
                     /translation="MVRPSRNVLALFLAVLAAVATIGAVLALYLRFEVAEQDAFADRA
                     VAAFDRPEVRRVVAREVVVQLIDRGSTDLVAARPVLEGVVEALVGTPPFQRLVRAAAA
                     QSHQVLFERDDNSLVFDLADVSTVVLSGMRSVSPDIARRVPDDVDARLVDVRERNFAG
                     DTLAAADAVRRLAVWLPVLAVLLLIGALVLAADRRRALTRYGLALGMTGIVAVVALST
                     GRAVLLDSIGGTDEIPRADLQAAVGGVWDSFLGDLRSLILIVALVGFVLASSILTVAD
                     PAAVRARVVALTRRPAGAPLAAARGIALVALGILVLLRPDDALRIVADAGGAGLV"
     gene            1025..1732
                     /locus_tag="VFY44_09970"
     CDS             1025..1732
                     /locus_tag="VFY44_09970"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX5910810.1"
                     /translation="MRRLAILSLAACMLAACGQDERPAGEEVLDGSAPLEARTTTEGS
                     ARTGSPRVVQVVPPGGVREQAGAADEEKKVRAENKRLRRELEDLEALEKARKRIQRRL
                     ARAGYDSNLVIGAGGLAWPVNGTVSSPFGPRWGRLHAGIDISAPAGRVIRAAQDGRVS
                     IRGPVSGYGNYTCVQHTKEFQTCYAHQSRFLVERGDDVVQGQAIGLVGCTGRCFGDHL
                     HFEVYVDNRPTDPMPYL"
     gene            complement(1734..2111)
                     /locus_tag="VFY44_09975"
     CDS             complement(1734..2111)
                     /locus_tag="VFY44_09975"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5910811.1"
                     /translation="MNSSIDLQHHDGIPVARLVGEVDLTRAPGIRVGLLRAVTNQDHG
                     LVLDLRDTTYLDSAGVNIIFELAERLSARQQRLAAVVPDRAIIERVLELVNMRSILES
                     HRTLEAAIGAIRALGPGEPAPGA"
     gene            complement(2108..3703)
                     /locus_tag="VFY44_09980"
     CDS             complement(2108..3703)
                     /locus_tag="VFY44_09980"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX5910812.1"
                     /translation="MARDTASQLRDLQQVTDAALSSLPLDQLLDELLERVRDILEVDT
                     VAILLLEEDGRVLAARAAKGLEEEVERGVRIPVGRGFAGRVAAERGPVQITDIDHADI
                     VNPLLREKGLQSLLGVPMLAEERLIGVLHVGALTKRLFPPDEIDLLQRAADRAALAIG
                     ARLYERQRGLAEEIQRSLFPRPLPDFPGISLAARYWPAASAPLGGDWYDAFMLPTGSL
                     GLVMGDVVGRGFRAAALMSQLRSALRAYAIDGNEPWELMTKLNMLLRRIDPGGSATLL
                     YLEFDSDAGQATLASAGHLPPLLRKGEEASFVDLPRAVPLGVVANPVYDERTLAIDPP
                     LELVLYTDGLMERAGEALEDGFERLREAGAPAAADPEELADRLVAALLPEGAATDDAA
                     VLVMAVHPLADELEVTLVAEPESAPLLRRILRRWLREHGASPDEVEELTLACGEASAN
                     AIEHAYPPEARSFLVEAHVEDGEIVMRIRDWGQWRPPRGAHRGKGMTLMEGLTDSVEV
                     DGGEEGTTVTLRRRPRPQEATAA"
     gene            3749..4648
                     /locus_tag="VFY44_09985"
     CDS             3749..4648
                     /locus_tag="VFY44_09985"
                     /inference="COORDINATES: protein motif:HMM:NF006128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX5910813.1"
                     /translation="MSEGYETILYRAGAATATITLNRPDRLNTIVPPMPEEVQHAVGR
                     ATRDHDVKVIVLRGAGRAFCAGYDFGQGFGHWDEYIQTGGEWDAGKDFAFATAQEVAP
                     TQQLMSVWRTPKPVIAQVHGWCVGGGSDFALCADLVVASEDAVIGTPYSRMWGAYLSG
                     MWIHRLGLARAKYHALTGRPLTGAQAAEVELVNEAVPFASLEARVAELAAELASIPSS
                     QLAAQKLVVNQAYENMGLASTQTLGPILDGLMRNTPEARAFIEKAEREGVRSVVEDRD
                     GPFGDYSQAPPELRPDPDNVIEP"
     gene            4645..5577
                     /locus_tag="VFY44_09990"
     CDS             4645..5577
                     /locus_tag="VFY44_09990"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX5910814.1"
                     /translation="MSHRDHLPQLDGGLFLTDGGLETSLIFHDGLDLPLFAAFGLLDN
                     DEGTDALRRYFEPYVELAGARGAGFVLESPTWRASPRWAAELGYSGDDLDALNRKAIA
                     LMEELRERYRSEAPMVISGCVGPHDDGYDPAELLSTAAAEDYHSTQIQTFASTAADMV
                     TAITMTYAAEAVGVTRAARAAGIPAAISFTVETDGRLPSGQALDEAIEQVDAETDGGP
                     AYYMLNCAHPTHFEAVVGGSGTWRHRIRGLRANASTRSHAELDEAEELDTGDPADLGA
                     RYAALADQLPELNVLGGCCGTDHRHVKAIAEAWP"
     gene            5568..6002
                     /locus_tag="VFY44_09995"
     CDS             5568..6002
                     /locus_tag="VFY44_09995"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX5910815.1"
                     /translation="MAVAMGQAAEQTNRLLLRARDTMDRHYAKPLDVPALARVALMSE
                     AHFIREFGAAFGETPHRYLQRRRVERAMFLLTQTDLPIMDVCLDVGFTSLGTFGRTFR
                     DIVGKTPTQFRQGNSPERVPLCFTMTWKRPSSFGEAAAKEQK"
     gene            6012..6476
                     /locus_tag="VFY44_10000"
     CDS             6012..6476
                     /locus_tag="VFY44_10000"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5910816.1"
                     /translation="MFNSFNISSIYVLDQDDALDFYVGKLGLEIGADIDMGFMRWLTV
                     QVPGHPERQVLLEKPNSPPLDEESVEQVRELVSKGAMGFTLGLTTDDAQKTFEELSAK
                     GVEFTQEPTEHFYGIDCGIRDPFGNNLRFSQPNPDMEVPSPEDLKKEFFVDG"
     gene            complement(6478..7647)
                     /locus_tag="VFY44_10005"
     CDS             complement(6478..7647)
                     /locus_tag="VFY44_10005"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX5910817.1"
                     /translation="MTARLPLDHARRLALHAQGLAVPPPAGQTVSDVVARIGCLQLDP
                     VGVVARSPLLVLRARMRGGTFASHDRALDRAAYTDRALFDYWCHEASLCHVGDLALHR
                     WAMRTYVERLTPARAHRARWLADNAEFVDHLVEDLRTRGPVRARELEDRSAAAWEWGH
                     WTDEVSSRQTIARMLDLLWMTGRVGVGARAGIERLWDVFERCVPEPEPERLDQGEVVR
                     RAALRAVGMLGVARAPHITAHFTRRRYPGLAEAVAGLEADGRLERVGVQGLRGEWWAR
                     PEDLERAARLEPGSRTVALSPFDNLICDRARAAELFGFDHRLEIYVPGPKRRWGYYVL
                     PVLHRERFVGRADLRAEDGVLRVLALHREPGRAAPAAIDRALRSLARWRGAELAA"
     gene            7673..8482
                     /locus_tag="VFY44_10010"
     CDS             7673..8482
                     /locus_tag="VFY44_10010"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5910818.1"
                     /translation="MGDYVHGYRERESERLADQAGTLVELLHGDTAYPAGATVLEAGC
                     GVGAQTVTLAARSPDARITSVDISEESLALARARVQEAGAANVELRRADILDPPFDPA
                     SFDHLFVCFVLEHLPEPGAALARLAGLVRPGGSVTVIEGDHGSAYFHPETAAARAAVD
                     TLVQLQAGAGGDALIGRRVYPLMVEAGLTDVHVSPRVVYADDSRPELVDGFTRRTFTA
                     MVEGVREPAIESGLVPAAEFDAGVLDLYRTAERGGTFSYTFFKGTGTVAAR"
     gene            complement(8479..>8829)
                     /locus_tag="VFY44_10015"
     CDS             complement(8479..>8829)
                     /locus_tag="VFY44_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910819.1"
                     /translation="RGFSLLILPTALSLEGPMLLAFTVFYGLDWIATVPPTVALANKR
                     FGRDVGLVAFGWIFGAHQIGAAAAAWAAGMVRDGSGTYDGVFLVSGLLCFAAAVMLAG
                     ARPRPRVVAEPAAA"
BASE COUNT         1264 a         3318 c         3191 g         1056 t
ORIGIN      
        1 tacaccagcc ccgctccgcc cgcatcggcc acgatccgga gcgcgtcgtc gggccgcagc
       61 agcaccagga tgcccagcgc caccagtgcg atgccgcgcg cggcggccag gggcgcgccg
      121 gcgggccggc gcgtgagcgc caccacgcgg gcccggacgg ctgccgggtc cgcgaccgtg
      181 aggatgctgg aggccagcac gaaccccacc agggccacga tcaggatcag cgaccgcagg
      241 tcgcccagga acgagtccca gacgcccccg acggcggcct ggaggtcggc tcgcgggatc
      301 tcgtcggtgc ccccgatgga gtccagcagc accgcccgcc cggtggacag cgccacgacg
      361 gccacgatgc ccgtcatgcc cagcgccagg ccgtagcggg tcagcgcccg gcggcggtcg
      421 gcggccagga ccagggcgcc gatcagcagg agcaccgcca gcacggggag ccacacggcc
      481 agccgcctga cggcgtcggc cgccgccagc gtgtccccgg cgaagttgcg ctcccggacg
      541 tccaccaggc gcgcgtccac gtcgtcgggc acccgccgcg cgatgtcggg cgagacgctg
      601 cgcatgcccg acagcaccac cgtgctcacg tcggccaggt cgaacaccag cgagttgtcg
      661 tcgcgctcga acagcacctg atgactctgc gcggccgcgg cccggaccag gcgctggaac
      721 ggcggcgtgc ccaccagcgc ctcgaccacc ccctccagca ccggccgcgc cgccaccagg
      781 tccgtcgagc cgcggtcgat cagctgcacc acgacctcac gcgccaccac gcggcggacc
      841 tcgggccggt cgaacgccgc gaccgcgcgg tcggcgaacg cgtcctgctc ggccacctcg
      901 aagcgcaggt acagcgccag caccgcgccg atcgtcgcca ccgcggccag caccgccagg
      961 aacagggcca gcacgttccg cgacggtcgc accatggctg tgcaccctaa gtcagactgg
     1021 aagcgtgaga cgcctcgcga tcctctccct cgccgcctgc atgctcgccg cctgcggcca
     1081 ggacgagcgt cccgccggcg aggaggtgct ggacggctcc gcgccgctgg aggcgcgcac
     1141 caccaccgag ggcagcgccc ggacgggctc gccgcgcgtg gtccaggtgg ttcccccggg
     1201 tggcgtccgt gagcaggccg gcgcagccga cgaggagaag aaggtgcgtg ccgagaacaa
     1261 gcggctgcgc cgtgagctcg aggacctgga ggcgctggag aaggcgcgca agcggatcca
     1321 gcggcgactg gcccgtgccg gttacgactc gaacctggtc atcggcgcgg gcggcctggc
     1381 gtggcccgtc aacggcacgg tcagctcacc gttcggcccg cgctggggcc gcctgcacgc
     1441 gggcatcgac atctcggcgc cggccgggcg cgtgatccgg gcggcccagg acgggcgggt
     1501 gtccatccgg gggccggtca gtgggtacgg caactacacc tgcgtccagc acaccaagga
     1561 gttccagacg tgttacgccc accagtcgcg cttcctggtc gagcggggcg acgacgtggt
     1621 ccagggacag gccatcggcc tggtgggctg caccggccgc tgcttcggcg accacctgca
     1681 cttcgaggtc tacgtggaca accgcccgac cgacccgatg ccctacctgt gagctaggcc
     1741 ccgggcgccg gctcgcccgg gccgagcgcc cggatggcgc cgatcgcggc ctccagcgtg
     1801 cggtggctct ccaggatcga gcgcatgttc accagctcca ggacgcgctc gatgatggcc
     1861 cggtcgggca ccacggccgc gagccgctgc tggcgggcgg acaggcgctc ggccagctcg
     1921 aagatgatgt tcacgcccgc gctgtccagg taggtcgtgt cgcgcaggtc caggaccagc
     1981 ccgtggtcct ggttggtcac ggcccgcagc aggccgaccc ggatgccggg cgcgcgcgtc
     2041 aggtccacct cgccgaccag gcgggccacc gggatgccgt cgtggtgctg caggtcgatg
     2101 ctcgagttca tgcggcagtg gcctcctggg ggcgcgggcg ccggcgcagg gtaacggtcg
     2161 tgccctcctc cccaccgtcg acctcgaccg agtcggtgag cccctccatc agcgtcatcc
     2221 ccttcccccg gtgcgcacct cgcggcgggc gccactggcc ccagtcccgg atgcgcatga
     2281 cgatctcccc gtcctccacg tgcgcctcga ccaggaacga gcgcgcctcg ggcgggtagg
     2341 cgtgctcgat ggcgttcgcg gacgcctcgc cgcaggccag cgtgagctcc tcgacctcgt
     2401 ccggggaggc gccgtgctcg cgcagccagc ggcgcaggat gcgccgcagc agcggcgccg
     2461 actcgggctc cgccaccagg gtcacctcga gctcgtcggc cagcgggtgc acggccatga
     2521 ccaggacggc ggcgtcgtcg gtcgcggccc cctcggggag gagcgcggcg acgagccggt
     2581 cggccagctc ctcgggatcg gcggcggccg gcgccccggc ctcacgcagt cgctcgaagc
     2641 cgtcctccag cgcctcgccg gcccgctcca tgagcccgtc ggtgtacagg acgagctcca
     2701 gcggggggtc gatcgccagc gtccgctcgt cgtagacggg gttggcgacc acaccgagcg
     2761 ggacggcccg cgggaggtcg acgaagctgg cctcctcccc cttgcgcagc agcggcggca
     2821 ggtgcccggc ggaggccaac gtggcctgcc ccgcgtcgga gtcgaactcc aggtagagca
     2881 gcgtggcgct gccgcccgga tcgatgcgcc gcagcaacat gttgagcttg gtcatgagct
     2941 cccacggctc gttcccgtcg atcgcgtacg cgcgcagcgc gctgcgcagc tggctcatca
     3001 gcgcggccgc gcggaagccg cggccgacga catctcccat gaccagcccc agcgatcccg
     3061 tgggcagcat gaaggcgtcg taccagtcgc cgccgagcgg cgcggaggcg gcgggccagt
     3121 agcgggcggc cagcgagatg cccgggaagt ccggcagcgg tcgcgggaac aggctgcgct
     3181 ggatctcctc ggccaggccc cgctggcgct cgtacagccg cgccccgatg gccagcgccg
     3241 cccggtccgc ggcccgctgg agcaggtcga tctcgtcggg cgggaacagc cgcttggtca
     3301 gggcgcccac gtgcaggacg ccgatcaggc gctcctcggc cagcatcggc acgccgagca
     3361 gcgactgcag tcccttctcg cgcaggagcg ggttgacgat gtcggcgtgg tcgatgtcgg
     3421 tgatctggac cgggccgcgc tcggcggcca ccctgccggc gaagccgcgg ccgacgggga
     3481 tgcgcacgcc ccgctcgacc tcctcctcga gccccttcgc cgcgcgcgcg gccagcacgc
     3541 ggccgtcctc ctccagcagc aggatcgcga cggtgtcgac ctccaggatg tcgcgcacgc
     3601 gctcgagcag ctcgtccagc agctggtcca gcgggagcga ggacagcgcg gcgtcggtga
     3661 cctgctggag gtcgcgcaac tgagacgcgg tgtcacgggc catcctcgat ggccagcgta
     3721 cctgagcccg gtccggtaca agacgaggat gagcgagggc tacgagacga tcctctaccg
     3781 cgccggcgcg gccaccgcca cgatcacgct caaccggccc gaccgcctga acacgatcgt
     3841 gccgcccatg cccgaggagg tgcagcacgc cgtcggccgc gccacgcgcg accacgacgt
     3901 caaggtcatc gtcctccgtg gcgccgggcg ggccttctgc gccggctacg acttcggcca
     3961 ggggttcggc cattgggacg agtacatcca gaccggcggc gagtgggacg cgggcaagga
     4021 cttcgcgttc gccaccgcgc aggaggtcgc ccccacccag cagctgatga gcgtgtggcg
     4081 cacgcccaag ccggtgatcg cccaggtgca cggctggtgc gtgggcggcg gcagcgactt
     4141 cgccctgtgc gccgacctgg tcgtggccag cgaggacgcc gtgatcggga ccccctacag
     4201 ccgcatgtgg ggcgcctacc tgtcgggcat gtggatccac cgcctggggc tggcccgggc
     4261 caagtaccac gcgctgaccg gccggcccct gaccggcgcc caggcggccg aggtcgagct
     4321 ggtcaacgag gccgtgccct tcgcctcgct ggaggcgcgg gtggccgagc tggcggccga
     4381 gctggcgtcg atcccctcgt cgcagctggc ggcccagaag ctcgtggtca accaggccta
     4441 cgagaacatg ggcctggcct ccacccagac gctgggcccg atcctggacg gcctgatgcg
     4501 caacacgccc gaggcccggg cgttcatcga gaaggccgag cgcgagggtg tacggtcggt
     4561 cgtggaggat cgcgacggcc cgttcggcga ctacagccag gcgccgcccg agctgcgccc
     4621 ggaccccgac aacgtgatcg aaccgtgagc caccgagacc acctccccca gctggacgga
     4681 ggcctgttcc tgaccgacgg cgggctggag accagcctga tcttccacga cggcctggac
     4741 ctgccgctgt tcgccgcctt cggcctgctg gacaacgacg agggcaccga cgcgcttcgc
     4801 cgctacttcg agccctacgt ggagctggcc ggcgcccgcg gcgccggctt cgtgctcgag
     4861 agcccgacct ggcgggccag cccgcgctgg gccgccgagc tggggtactc cggcgacgac
     4921 ctggacgcgc tcaaccgcaa ggcgatcgcg ctgatggagg agctgcggga gcggtaccgg
     4981 tccgaggcgc ccatggtcat cagcgggtgc gtggggcccc acgacgacgg ctacgacccg
     5041 gccgagctgc tgtccacggc cgccgccgag gactaccact ccacccagat ccagacgttc
     5101 gcctcgaccg ccgcggacat ggtcaccgcg atcacgatga cctacgccgc cgaggccgtc
     5161 ggcgtgaccc gcgcggccag ggccgccggg atcccggcgg ccatctcgtt cacggtcgag
     5221 accgacggcc ggctgcccag cggccaggcg ctggacgagg caatcgagca ggtcgacgcg
     5281 gagacggacg gcggcccggc ctactacatg ctcaactgcg cccacccgac gcacttcgag
     5341 gccgtggtcg gcgggagcgg cacgtggagg caccggatcc gggggctgcg cgccaacgcc
     5401 tcgaccagga gccacgccga gctggacgag gccgaggagc tggacaccgg cgacccggcc
     5461 gacctgggag cgcggtacgc ggcgctggcc gaccagctcc cggagctcaa cgtgctgggc
     5521 ggctgctgcg gcaccgacca ccggcacgtg aaggcgatcg cggaggcgtg gccgtagcga
     5581 tgggtcaggc cgccgagcag accaaccggc tgctgcttcg cgcccgcgac accatggacc
     5641 gccactacgc gaagccgctg gacgtgcccg cgctggcgcg cgtcgcgctg atgtccgagg
     5701 cgcacttcat ccgcgagttc ggcgccgcgt tcggcgagac gccccaccgc tacctgcagc
     5761 ggcggcgggt cgagcgggcc atgttcctgc tgacccagac cgacctgccg atcatggacg
     5821 tgtgcctgga cgtcgggttc accagcctcg gcacgttcgg ccggacgttc cgcgacatcg
     5881 tcgggaagac gcccacgcag ttccgtcagg gcaactcgcc ggagcgggtg ccgctgtgct
     5941 tcacgatgac gtggaagcga ccgagcagtt tcggagaagc cgccgcgaag gagcaaaagt
     6001 aggttcgccc catgttcaac tcattcaaca tctcaagcat ctacgtgctg gaccaggacg
     6061 acgcgctgga cttctacgtc ggcaagctcg ggctggagat cggggccgac atcgacatgg
     6121 gcttcatgcg ctggctgacc gtgcaggtgc ccggccaccc cgagcgccag gtcctgctgg
     6181 agaagcccaa ctcgccgccc ctggacgagg agtccgtgga gcaggttcgc gagctggtgt
     6241 ccaagggcgc catgggcttc acgctgggcc tgaccaccga cgacgcgcag aagacgttcg
     6301 aggagctgtc ggccaagggc gtggagttca cgcaggagcc gaccgagcac ttctacggca
     6361 tcgactgcgg catccgcgac ccgttcggta acaacctgcg cttcagccag ccgaaccccg
     6421 acatggaggt gccgtccccg gaggacctca agaaggagtt cttcgtcgac ggctgactca
     6481 ggccgccagc tcggcgccgc gccagcgcgc cagcgatcgc aacgcgcggt cgatggccgc
     6541 gggcgcggcg cggccgggct cgcggtgcag cgccaggacg cgcagcacgc cgtcctcggc
     6601 ccgcaggtcg gcccggccca cgaagcgctc gcggtgcagg accgggagca cgtagtagcc
     6661 ccagcggcgc ttgggccccg gcacgtagat ctccagccgg tggtcgaagc cgaacagctc
     6721 cgccgcccgc gcccggtcgc agatcaggtt gtcgaacggg gacagcgcga cggtgcggct
     6781 gcccggctcc aggcgcgccg cccgctccag gtcctcgggc cgcgcccacc actcgccgcg
     6841 caggccctgg acgcccacgc gctccagccg gccgtcggcc tccaggccgg ccacggcctc
     6901 cgccaggccg gggtagcgcc ggcgcgtgaa gtgcgcggtg atgtgcggcg cccgcgccac
     6961 gcccagcatg cccaccgccc gcagcgccgc ccggcggacg acctcgccct ggtccaggcg
     7021 ctcgggctcg ggctcgggga cgcagcgctc gaacacgtcc cacaggcgct cgatcccggc
     7081 cctggccccc acccccacgc ggccggtcat ccacagcagg tccagcatcc gggcgatggt
     7141 ctggcgcgag gacacctcgt cggtccagtg cccccactcc cacgccgccg ccgaccggtc
     7201 ctccagctcg cgcgcgcgta cggggccgcg cgtacggagg tcctcgacca ggtggtccac
     7261 gaactcggcg ttgtcggcca gccagcgggc ccggtgcgcg cgggcgggcg tcagccgctc
     7321 gacgtacgtc cgcatggccc agcggtgcag cgccaggtcg ccgacgtggc acaggctggc
     7381 ctcgtggcac cagtagtcga acagcgcgcg atcggtgtac gcggcccggt ccagcgcgcg
     7441 gtcgtggctg gcgaaggtcc cgccgcgcat gcgggcgcgg agcaccagga ggggcgaccg
     7501 cgcgaccacc cccacggggt ccagctgcag gcagccgatc cgggccacga cgtccgacac
     7561 cgtctggccg gcgggcgggg gcacggccag gccctgcgcg tgcagcgcca gccggcgtgc
     7621 gtggtcgagg ggcaggcgcg ccgtcacggc aggcgaggct agcctgccgc ccgtgggcga
     7681 ctacgtgcac gggtaccgcg agcgcgagag cgagcgactg gccgaccagg cgggcacgct
     7741 ggtggaactg ctgcacggcg acacggcgta ccccgccggc gccaccgtgc tggaggcggg
     7801 ctgcggggtg ggcgcgcaga cggtcaccct ggccgcgcgc agcccggacg cccggatcac
     7861 ctccgtcgac atctccgagg agtcgctggc cctcgcccgc gcgcgtgtcc aggaggcggg
     7921 cgcggccaac gtggagctgc gccgtgccga catcctggac ccgccgttcg acccggcgtc
     7981 gttcgaccac ctgttcgtgt gcttcgtcct ggagcacctg cccgagcccg gcgccgcgct
     8041 ggcgcggctg gccggcctgg ttcgccccgg cggctcggtc acggtgatcg agggcgacca
     8101 cgggtcggcc tacttccacc ccgagaccgc cgcggcgcgc gccgccgtcg acaccctggt
     8161 gcagctgcag gcgggcgccg gcggcgacgc cctgatcggc cgccgcgtgt acccgctgat
     8221 ggtggaggcc gggttgaccg acgtgcacgt gtcgccgcgg gtggtctacg cggacgacag
     8281 ccggcccgag ctggtcgacg gcttcacccg caggacgttc accgccatgg tcgagggcgt
     8341 gcgcgagccc gcgatcgagt ccggcctggt cccggccgcc gagttcgacg ccggcgtgct
     8401 cgacctgtac cgcacggccg agcggggcgg cacgttctcg tacacgttct tcaagggcac
     8461 gggcacggtc gccgcccgct aggcggcggc cggctcggcc accacgcgcg ggcggggccg
     8521 ggcgcccgcc agcatcaccg cggcagcgaa gcacagcagc cccgagacca ggaacacccc
     8581 gtcgtaggtg cccgagccgt cgcgcaccat ccccgcggcc caggcggccg cggcggcgcc
     8641 gatctggtgc gcgccgaaga tccagccgaa cgccaccagg ccgacgtcgc ggccgaagcg
     8701 cttgttggcc agggccaccg tgggcggcac cgtggcgatc cagtccagcc cgtagaacac
     8761 ggtgaaggcc agcagcatcg gcccctccag gctcagcgcg gtgggcagga tcagcagcga
     8821 gaagccacg
//
LOCUS       DASUPW010000443         2778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1625810, whole genome shotgun sequence.
ACCESSION   DASUPW010000443 DASUPW010000000
VERSION     DASUPW010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625810"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VFY44_10020"
     CDS             <1..305
                     /locus_tag="VFY44_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910820.1"
                     /translation="GAAKAKAEPKAKASKSKAKATTTRAKSSTKSTAAKAKPKAKATA
                     SKAKAKPKAAATKAKPKAKASTTRAKSTTTKAKAKPKAASKSKAKPASRSRAKAKK"
     gene            318..1106
                     /locus_tag="VFY44_10025"
     CDS             318..1106
                     /locus_tag="VFY44_10025"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5910821.1"
                     /translation="MVAVVTGASSGIGEAVAHRLASEPGAKLVLIARREDRLRALAAS
                     LPAPTTYVAVDVTDDDAPQKVLDHVEAEHGRLDLLVNNAGAGFRGTFAEKGYANVKRT
                     MDLNFDAVVRMTEALLPLLRRSAPSSIVMVSSTAGRISRPGSGAYSASKFALAGWTDA
                     LRSEELKSGVHVGNVLPGFISTEGFPQAELTGKALTRWAVSTPDKAAEAVYEAGPGGK
                     AERYVPRAYVLVGIVRMLFPGLLRRVMDGGAAKSLATKTGEGTE"
     gene            1103..2554
                     /locus_tag="VFY44_10030"
     CDS             1103..2554
                     /locus_tag="VFY44_10030"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5910822.1"
                     /translation="MTTREVVIVGAGFGGIGMARELVRRGVTDFELLERASDVGGTWR
                     ANTYPGCQCDIPSNLYSFSFAPKPDWTHAYPEQPQILEYLRHCDDRFGVRPHLRLDCE
                     VTDARWDDDAAVWDIETSQGPLRAHTLIAAPGLLSNPRVPERFRAFEGEVFHSAQWDH
                     DTPLDGRRVGMVGTGASAIQIGPAIQPRVRSLTVFQRSAPWIIPHPDRAIPDAMKRLY
                     TRVPAVQRLARGAIYAAHETMAPGLTYFPRLMKGQEANARLLRRRQVKDPELLAKVTP
                     DYEIGCKRILLSSDWYPMLAEPNVELVTDGIEEVRAHSIVTAGGTEHELDALVMATGF
                     DPVNIPLAQRLHGRDGRSLTDVWGGSPQAFLGTAVAGFPNLFMIYGPNLNQGHTSMVY
                     LLESQIAYVGQALERLGRLGAMEVRPEVQQRYNDDLQRRLAGSVWNNGGCSSWYLDEN
                     GHNSIQWPGFSFSFRNRTRRFELSDYRLRPAVA"
BASE COUNT          433 a         1009 c          992 g          344 t
ORIGIN      
        1 cgggcgcggc gaaggccaag gccgagccga aggcgaaggc ctcgaagtcg aaggccaagg
       61 ccaccacgac ccgggccaag tcctcgacca agagcaccgc cgcgaaggcc aagccgaagg
      121 cgaaggccac cgcgagcaag gcgaaggcca agcccaaggc cgcggccacc aaggccaagc
      181 cgaaggccaa ggcctcgacg accagggcca agagcacgac caccaaggcg aaggccaagc
      241 ccaaggctgc gtccaagtcc aaggccaagc cggccagccg cagcagggcc aaggcgaaga
      301 agtaggctcg cgacggcatg gtggccgtcg tcacaggagc atcgagcggg atcggcgagg
      361 cggtggccca ccgcctcgcc tccgagcccg gggcgaagct ggtgctgatc gcgcgccgcg
      421 aggaccggct gcgggcgctg gccgccagcc tgcccgcacc caccacctac gtcgcggtcg
      481 acgtgaccga cgacgacgcg ccgcagaagg tgctggacca cgtcgaggcc gagcacgggc
      541 gcctggacct gctggtcaac aacgccggcg ccggcttccg cggcacgttc gccgagaagg
      601 gctacgccaa cgtcaagcgc acgatggacc tgaacttcga cgccgtggtg cgcatgaccg
      661 aggcgctgct gccgctgctg cgccgctcgg cccccagctc gatcgtgatg gtctccagca
      721 ccgcgggccg gatctcgcgg ccgggctcgg gcgcctactc ggccagcaag ttcgcgctgg
      781 cgggctggac cgacgcgctg cgttcggagg agctgaagag cggcgtgcac gtgggcaacg
      841 tgctgccagg gttcatcagc accgagggct tcccccaggc cgagctgacc gggaaggcgc
      901 tgacgcggtg ggccgtgtcc acgcccgaca aggcggccga ggcggtgtac gaggccgggc
      961 ccggcggcaa ggccgagcgc tacgtcccgc gcgcctacgt gctggtgggc atcgtgcgga
     1021 tgctgttccc gggcctgttg cgccgggtca tggacggcgg cgccgccaag tcgctggcca
     1081 cgaagacggg agagggcacc gagtgaccac gcgcgaggtc gtgatcgtcg gtgccggctt
     1141 cgggggcatc ggcatggccc gtgagctggt ccgccgcggg gtcaccgact tcgagctgct
     1201 ggagcgcgcg agcgacgtcg gcgggacgtg gcgggccaac acctaccccg gctgccagtg
     1261 cgacatcccc tcgaacctct actcgttctc gttcgcgccc aagccggact ggacccacgc
     1321 ctaccccgag cagccccaga tcctggagta cctgcgccac tgcgacgacc gcttcggcgt
     1381 ccgcccgcac ctgcggctgg actgcgaggt caccgacgcc cgctgggacg acgacgcggc
     1441 cgtgtgggac atcgagacgt cgcaggggcc gctgcgcgcc cacacgctga tcgccgcgcc
     1501 cggcctgttg agcaacccgc gcgtgccgga gcggttccgg gccttcgagg gcgaggtgtt
     1561 ccactcggcc cagtgggacc acgacacgcc gctcgacggg cgccgggtgg gcatggtggg
     1621 cacgggcgcg tcggccatcc agatcggccc cgcgatccaa ccccgcgtgc gcagcctgac
     1681 cgtcttccag cgctcggcgc cgtggatcat cccgcacccc gaccgcgcga tcccggacgc
     1741 catgaagcgg ctgtacaccc gcgtgcccgc ggtccagcgg ctggcgcgcg gggccatcta
     1801 cgcggcgcac gagaccatgg cgccggggct gacctacttc ccccggctga tgaagggcca
     1861 ggaggccaac gcgcgcctgc tgcgccggag gcaggtcaag gaccccgagc tgctggccaa
     1921 ggtcacgccc gactacgaga tcggctgcaa gcggatcctg ctgtccagcg actggtatcc
     1981 gatgctggcc gagcccaacg tcgagctggt cacggacggc atcgaggagg tgcgcgcgca
     2041 ctcgatcgtc acggccggcg ggaccgagca cgagctggac gcgctggtca tggccaccgg
     2101 cttcgacccg gtgaacatcc ccctggcaca gcgcctgcac ggccgcgacg ggcggtcgct
     2161 gaccgacgtc tggggcgggt cgccgcaggc cttcctgggc accgcggtcg cgggctttcc
     2221 caacctgttc atgatctacg gccccaacct caaccagggg cacacgtcca tggtctacct
     2281 gctggagtcg cagatcgcat acgtgggcca ggcgctggag cggctgggtc gcctgggggc
     2341 gatggaggtc cggcccgagg tccagcagcg ctacaacgac gacctccagc ggcggctggc
     2401 cggcagcgtc tggaacaacg gcggctgctc gtcgtggtac ctggacgaga acggccacaa
     2461 ctcgatccag tggcccggct tctcgttctc cttccgcaac cgcacccgcc ggttcgagct
     2521 gtccgactac cgcctgcggc ccgcagtcgc ctgagacctt ggggggcgct ttggggcggt
     2581 cccccgatgc tgggggccgc gccccggtgc accatggccc catggaagcc ctgacccccg
     2641 cgctggccgt ccacgtggtt cgcgacggcc gcgccagcgc cggcgcctac tcgctgctcg
     2701 aggcgcgcgc gccggctgga ttcgccatcc cccacaacgt caaccgcatg tgcgccggcg
     2761 tcctgcaggt ggtggggg
//
LOCUS       DASUPW010000444         2785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1625946, whole genome shotgun sequence.
ACCESSION   DASUPW010000444 DASUPW010000000
VERSION     DASUPW010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625946"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VFY44_10035"
     CDS             <1..386
                     /locus_tag="VFY44_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910823.1"
                     /translation="LKGLSPIAAVGATIEDVVKDVAFADSRGRGVGLRSGRYTLGRDL
                     RLGGLSYVPGLRLTGRVARFESDRRRRGDVRVSGPRGYRGVLRFRGRKVSGRLGGRRV
                     GARLVLGAAARSAGSASAPYRPGHQ"
     gene            complement(282..1217)
                     /locus_tag="VFY44_10040"
     CDS             complement(282..1217)
                     /locus_tag="VFY44_10040"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5910824.1"
                     /translation="MDPAIVAFGLGVGVLVGMTGIGGGSLMTPLLILVFGVKPVTAVG
                     TDLAYGAVTKTVGGIKHWRQKTVDFPLSVWMGIGSVPSAIGGVYALGAIERAYGDAFD
                     DTLILALAVTLLFCGVMTLARALFLKRIAGRERESVQMGRSEKVRAVVLGVVVGFVLG
                     VTSAGSGALIAVGLIMLFHLVPQRVVGTDVFHAAILLWAAALAHIVAGNVDFLLAGNI
                     LLGSVPGVWAGSHLSVRIPVATLRKVLALVLIGSGLGLLTKAGVGVPAWTLAAFPVAV
                     SLLVAWSVRSRRAARAARRGAEHQPRPYPPAPQAP"
     gene            complement(1222..2475)
                     /locus_tag="VFY44_10045"
     CDS             complement(1222..2475)
                     /locus_tag="VFY44_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017488255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX5910825.1"
                     /translation="MAVAEAPMLRLATSGSVDDGKSTLIGRLLYDSKQVLSDQLEHVE
                     RVSEKRGDGYVNLALLTDGLRAEREQGITIDVAYRYFQTARRKFIIADTPGHVQYTRN
                     MVTGASTADLSIVLVDARAGVVEQSKRHAFIASLLRIPHLVVAVNKMDLVDFDEAVFD
                     DIRADFTDFASELGFTEITFIPVSALEGDNVVDRSERTPWYDGRSLLEHLETVEVASD
                     RNLEELRLPVQWVIRPQTAEHHDYRGYAGRLASGMLTPGDEVVVLPSGHRTRVEAVDG
                     AEGELDQAFPPMSVTVRLEDDIDVSRGDLIADPESAPEPVRELTADVCWMADAPLAPR
                     GRYALKHTTRTVRAIVDEVQTRVDVHTLEDDPAESLELNDIGRVRLRLGAPLTVDPYA
                     RNRDTGSFILIDEATGDTVGAGMIR"
     gene            complement(2478..>2785)
                     /locus_tag="VFY44_10050"
     CDS             complement(2478..>2785)
                     /locus_tag="VFY44_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002855962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfate adenylyltransferase small subunit"
                     /protein_id="HEX5910826.1"
                     /translation="DLEVPSIYYAHEREVFERDGMLYAASPHIQLQDGEDVFTESVRY
                     RTVGDLTVTGAVCSTAATLDDVVGEIAATRVTERGETRADDRVTEAAMEDRKKEGYF"
BASE COUNT          396 a         1012 c         1003 g          374 t
ORIGIN      
        1 cgctcaaggg cctgagcccc atcgccgcgg tgggcgcgac gatcgaggac gtggtcaagg
       61 acgtggcctt cgccgactcg cgcggccgcg gcgtgggcct gcgctcgggg cggtacacgc
      121 tggggcgcga cctgcgcctc ggcggcctgt cctacgtgcc ggggctgagg ctgaccggcc
      181 gggtcgcccg cttcgagagc gacaggcgcc ggcgcggcga cgtccgcgtc agcgggccgc
      241 gcggctaccg cggcgtgctg cgcttccgcg ggcggaaggt gtcagggcgc ctggggggcc
      301 ggcgggtagg ggcgcggctg gtgctcggcg ccgcggcgcg cagcgcgggc agcgcgtcgg
      361 ctccgtaccg accaggccac cagtagggac accgccaccg ggaacgccgc gagcgtccac
      421 gcgggcaccc cgacgcccgc cttggtcagc agccccaggc cggacccgat cagcaccagc
      481 gccagcacct tccgcagcgt cgcgacgggg atccgcaccg acaggtggct gcccgcccag
      541 acgcccggca ccgaccccag caggatgttg ccggccagca ggaagtccac gttgccggcg
      601 acgatgtggg ccagggccgc ggcccacagc aggatcgcgg cgtggaacac gtcggtgccc
      661 accacgcgct gggggaccag gtggaagagc atgatcagcc cgacggcgat cagcgcgccg
      721 ctgccggccg aggtcacgcc gaggacgaac cccaccacca cgcccaggac caccgcgcga
      781 accttctcgg accggcccat ctgcacggac tcgcgctcgc gcccggcgat gcgcttcagg
      841 aagagcgccc gggccagcgt catcacgccg cagaacagca gcgtcacggc cagcgccagg
      901 atcagcgtgt cgtcgaacgc gtcgccgtag gcgcgctcga tcgcgccaag cgcgtagacg
      961 ccgccgatgg ccgagggaac cgagccgatt cccatccaca ccgacagcgg gaagtccacg
     1021 gtcttctgcc gccagtgctt gatgccgccg acggtcttgg tcaccgcccc gtaggccagg
     1081 tcggtgccca cggcggtcac cggcttgacg ccgaacacca ggatcagcag cggggtcatc
     1141 agcgagccgc ccccgatccc ggtcatgccc accagcaccc cgaccccgag gccgaacgcg
     1201 acgatcgccg ggtccacggc cctagcggat catgccggcg cccacggtgt cgccggtggc
     1261 ctcatcgatc aggatgaagc tcccggtgtc gcggttgcgg gcgtacgggt ccaccgtcag
     1321 cggcgcgccc aggcgcaggc gcacgcggcc gatgtcgttc agctccaggc tctcggccgg
     1381 gtcgtcctcc agggtgtgca cgtccacgcg ggtctggacc tcgtccacga tcgcgcgcac
     1441 ggtgcgggtg gtgtgcttga gcgcgtagcg cccacgcggg gccagcggcg cgtcggccat
     1501 ccagcacacg tccgccgtca gctcgcgcac cggctcgggc gcgctctcgg ggtcggcgat
     1561 caggtcgccg cgcgagacgt cgatgtcgtc ctccaggcgc acggtgaccg acatcggcgg
     1621 gaacgcctgg tccagctcgc cctcggcgcc gtccacggcc tcgacgcggg tgcggtgacc
     1681 ggacggcagg accaccacct cgtcgcccgg cgtcagcatg ccggaggcca gccggccggc
     1741 gtagccgcgg tagtcgtggt gctcggccgt ctgcgggcgg atcacccact gcacgggcag
     1801 gcggagctcc tcgaggttgc ggtcgctggc cacctcgacg gtctccaggt gctccagcag
     1861 cgagcggccg tcgtaccagg gcgtgcgctc ggagcggtcg accacgttgt cgccctccag
     1921 cgcgctgacc gggatgaacg tgatctcggt gaagcccagc tcggacgcga agtcggtgaa
     1981 gtccgcccgg atgtcgtcga acacggcctc gtcgaagtcg accaggtcca tcttgttcac
     2041 cgccaccacc aggtgcggga tgcgcaacag cgaggcgatg aaggcgtggc gcttggactg
     2101 ctcgaccacg ccggcgcggg cgtccacgag cacgatcgaa aggtccgctg tcgacgcccc
     2161 cgtgaccatg ttgcgcgtgt actgaacgtg gcccggcgtg tcggcgatga tgaacttgcg
     2221 ccgcgcggtc tggaagtagc ggtaggccac gtcgatcgtg atgccctgct cgcgctcggc
     2281 gcgcaggccg tcggtcagca gcgccaggtt cacgtagccg tcgccacgct tctcggacac
     2341 gcgctcgacg tgctcgagct ggtcggacag cacctgcttc gagtcgtaga gcagccgccc
     2401 gatcagggtc gacttgccgt cgtccacgga gccggaggtg gccaggcgga gcatgggtgc
     2461 ttccgcgacg gccatggcta gaagtacccc tccttcttgc ggtcctccat ggcggcctcg
     2521 gtcacgcggt cgtcggcgcg ggtctcgccg cgctcggtga cccgcgtggc ggcgatctcg
     2581 ccgaccacgt cgtccagggt cgcggcggtg gagcacacgg cgccggtcac ggtcaggtcg
     2641 ccgaccgtgc ggtagcggac cgactcggtg aacacgtcct cgccgtcctg cagctggatg
     2701 tggggcgagg cggcgtagag catcccgtcg cgctcgaaca cctcgcgctc gtgcgcgtag
     2761 tagatcgacg gcacctccag gtcct
//
LOCUS       DASUPW010000445         2844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1764864, whole genome shotgun sequence.
ACCESSION   DASUPW010000445 DASUPW010000000
VERSION     DASUPW010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1764864"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VFY44_10055"
     CDS             complement(<1..354)
                     /locus_tag="VFY44_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910827.1"
                     /translation="MDHDDPLTPAASGLDRRNFLGIAGGGLLLCTVGGQKIEVKNAAD
                     VAKVDKAAASVKRPAAAQKKKGSKRERVDDMRFGTPGPAPGGRRKEYWIEAKPVRWNV
                     APTGRDEWMGKRVPRR"
     gene            482..1153
                     /gene="nucS"
                     /locus_tag="VFY44_10060"
     CDS             482..1153
                     /gene="nucS"
                     /locus_tag="VFY44_10060"
                     /inference="COORDINATES: protein motif:HMM:NF002876.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HEX5910828.1"
                     /translation="MRLIVARCEVRYTGRLTALLPEALRLLMIKSDGSVMVHADTGGF
                     KPQNWMTPPTVIEESDDRIVVRKRAGTTEDRLEIDIAEVVSDVTHDMGEAAALEKEGV
                     EKDLQALLADAPEHCGEGFRLVRREWPTDIGPVDLMCRDSDDGWVAVEIKRVATIDAV
                     EQLTRYLERLATEPGFATCRGVLAATQIKPQARVLAESRGIDCVEVDPAVLRGEREPD
                     LVLFG"
     gene            1157..2224
                     /locus_tag="VFY44_10065"
     CDS             1157..2224
                     /locus_tag="VFY44_10065"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5910829.1"
                     /translation="MAEGRQGAVAYRTVLLAGGLLVLGLLFEQLVSLLLAVLITVIVA
                     IALSSFADRLERRLKIPRPIGALLGIVLGLGVLAGIVALIIPPFIDQTNQFVDDVPGI
                     VDDLRDQVADLTGAEPGEISDRVQDFADKQAEDPGKLVGPVTSIGFSAAGVLGALLLV
                     LITAYYMAINPDPLLNGVRRLIPPRHRDEADRVMRRLRTSWIGWIQGVVVDMVVSGIL
                     LYIGLTIIGLDFAIFFAVLTALLSVIPYFGAIISGLPPVLFALTDSPGKALLVIVVYT
                     LVQQFEGNVTIPLVMARTVKLHPAVIAVGVVVVGQLFGFIGLIVAVPVLSMLTIMVEE
                     LWVKPMEEAERSRGTPEITRA"
     gene            2308..2502
                     /locus_tag="VFY44_10070"
     CDS             2308..2502
                     /locus_tag="VFY44_10070"
                     /inference="COORDINATES: protein motif:HMM:NF039788.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6458 family protein"
                     /protein_id="HEX5910830.1"
                     /translation="MSIGASIFLFATGAILRYAITAEVSGVDIATVGTILMVAGVIAL
                     LASLALAFAGPNNPRDDLRR"
     gene            complement(2504..>2844)
                     /locus_tag="VFY44_10075"
     CDS             complement(2504..>2844)
                     /locus_tag="VFY44_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5910831.1"
                     /translation="IAAGARGYVLKSSPVSDLIRSIRSIAGGQAYVDPALSPTLLMDP
                     GAPEAPLPERERQILQHLAEGLRTEEVAARVGLSAETVKADTKRAIHRLEATGRVHAV
                     ANALRRSYIT"
BASE COUNT          387 a          988 c         1006 g          463 t
ORIGIN      
        1 ccggcgcggg acgcgcttgc ccatccattc gtcgcggccg gtgggcgcga cgttccagcg
       61 caccggcttc gcctcgatcc agtactcctt gcggcgcccg cccggcgcgg gcccgggtgt
      121 gccgaagcgc atgtcgtcca cgcgctcgcg cttggagccc ttcttcttct gggccgccgc
      181 ggggcgcttg acggaggccg cggccttgtc gaccttggcc acgtcggccg cgttcttgac
      241 ttcgatcttc tgcccgccca cggtgcacag cagcaggcca ccgcccgcga tgccgaggaa
      301 gttgcgccgg tcgaggccgg aggcggcggg tgtcagaggg tcgtcgtggt ccatcgcgat
      361 gtgtcgcccg gtccacccct gccatggccg gaagacgtga ccttacaacg gatctggcac
      421 caaaacgctc cggttaaccg aaccttcgtc ccgtccgcgc cggcgcctac cgtcgggacc
      481 tgtgcgcttg atcgtcgccc gctgcgaggt ccgttacacg ggccgcctga ccgcgctcct
      541 cccggaggcg ctccggctgc tgatgatcaa gtcggacggc tcggtcatgg tccacgccga
      601 caccggcggc ttcaagccgc agaactggat gaccccgccg acggtgatcg aggagtccga
      661 cgaccggatc gtcgtgcgca agcgcgccgg cacgaccgag gaccggctgg agatcgacat
      721 cgccgaggtg gtctccgacg tgacccacga catgggcgag gccgccgcgc tggagaagga
      781 gggggtcgag aaggacctcc aggcgctgct ggccgacgcc cccgagcact gtggtgaggg
      841 cttccgcctc gtccgccgcg aatggcccac cgacatcggc cccgtcgacc tgatgtgccg
      901 cgactccgac gacggctggg tcgccgtgga gatcaagcgc gtggccacca tcgacgcggt
      961 cgagcagctg acgcgctacc tcgagcggct ggccaccgag cccggcttcg ccacctgccg
     1021 cggcgtcctg gccgccaccc agatcaagcc ccaggcgcgc gtgctggccg agtcgcgggg
     1081 gatcgattgc gtcgaggtcg accccgccgt cctgcgcgga gagcgcgagc ccgacctcgt
     1141 tctcttcggg taggtgatgg ccgaggggcg ccagggggcc gtcgcctacc gcacggtcct
     1201 gctggccggc ggcctcctgg tgctggggct gctgttcgag cagctcgtgt ccttgctgct
     1261 cgcggtgttg atcacggtga tcgtggccat cgcgctgtcc tcgttcgccg accggctcga
     1321 gcgccggctg aagatcccgc ggccgatcgg ggcgctgctg gggatcgtgc tcgggctggg
     1381 cgtgctggcc gggatcgtgg cgctgatcat cccgccgttc atcgaccaga ccaaccagtt
     1441 cgtggacgac gtgcccggca tcgtcgacga cctgcgcgac caggtcgcgg acctgaccgg
     1501 agccgagccg ggcgagatct ccgaccgcgt gcaggacttc gccgacaagc aggccgagga
     1561 cccgggcaag ctggtcgggc cggtcacgtc gatcgggttc agcgccgccg gggtgctggg
     1621 cgcgctgctc ctggtcctga tcaccgccta ctacatggcc atcaacccgg acccgctgct
     1681 gaacggggtg cggcggctga tcccgccccg tcaccgcgac gaggccgacc gggtgatgcg
     1741 ccggctgcgc acctcgtgga tcggctggat ccagggggtg gtggtcgaca tggtcgtctc
     1801 ggggatcctg ctctacatcg ggctgacgat catcggcctg gacttcgcga tcttcttcgc
     1861 cgtgctgacc gcgctgctct cggtgatccc ctacttcggc gccatcatca gcgggctgcc
     1921 gccggtgctg ttcgcgctca ccgactcacc cggcaaggcg ctgctggtga tcgtggtcta
     1981 cacgctggtc cagcagttcg agggcaacgt gacgattccg ctggtcatgg cgcggacggt
     2041 caagctgcac cccgccgtga tcgcggtggg cgtggtggtg gtgggtcagc tgttcgggtt
     2101 catcggcctg atcgtggccg tgcccgtgct ctcgatgctc acgatcatgg tcgaggagct
     2161 gtgggtgaag ccgatggagg aagccgagcg ctcgcgggga acgccggaga tcacgagagc
     2221 ttgaacgccg cgatcacgat cgacgccgcg acgaagatca cgatcagaac ctgcatgtag
     2281 atccagaaac gaggcacaat ccccactatg tcaatcggag cttcgatctt cctgttcgcc
     2341 accggcgcca tcctgcggta cgccatcacg gccgaggtca gcggcgtcga catcgcgacg
     2401 gtgggcacga tcctgatggt ggccggcgtg atcgcgctgc tggccagcct ggcgctggcg
     2461 ttcgccgggc cgaacaaccc ccgcgacgac ctgcggcgct gacctaggtg atgtaggagc
     2521 ggcgcagggc gttggccacc gcgtgcacgc ggccggtggc ctcgagccgg tggatcgccc
     2581 gcttggtgtc ggccttgacg gtctcggccg acagccccac gcgcgcggcc acctcctcgg
     2641 tgcgcagacc ctcggccagg tgctgcagga tctggcgctc gcgctcgggc agcggcgcct
     2701 cgggcgcgcc cgggtccatc agcagcgtcg gtgacagcgc cgggtccacg taggcctggc
     2761 cgcccgcgat ggagcggatg gaccggatca ggtcggagac gggcgagctc ttgagcacgt
     2821 atccccgcgc gcccgcggcg atcg
//
LOCUS       DASUPW010000446         2571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1072173, whole genome shotgun sequence.
ACCESSION   DASUPW010000446 DASUPW010000000
VERSION     DASUPW010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072173"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VFY44_10080"
     CDS             complement(<1..828)
                     /locus_tag="VFY44_10080"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5910832.1"
                     /translation="MRVGVVGLGYWGPNLARNFDRLPGVELAWLCDADDERRERQALA
                     FPGARQSADLDELLADPALDAVCVATPVPTHAAIAERVLAAGKHCFVEKPLAQTVADA
                     ERVVALAAEADRVLMVGHLLEYHPGLEMLNRLATDGELGDIHYIYGNRLNLGKLRAEE
                     NALWSLGAHDVSVLLRLAGEEPYEVSAKGESYMRPGVEDVVFCFLRFPSGLAAHLHLS
                     WLDPHKERRFTVVGSKKMATFDDMDVERKLTVYDKGFDQDSTSYGEYITRSGDTYSPR
                     "
     gene            complement(840..2087)
                     /locus_tag="VFY44_10085"
     CDS             complement(840..2087)
                     /locus_tag="VFY44_10085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX5910833.1"
                     /translation="MKIGVIGLGYVGLPLALAFAEEGHTVAGVDSDSRRAAALGRHES
                     YIEDVSSERLAAVGDRITAGTRYAELADCDAVMIAVPTPLTTNREPDLGPLISAGTSL
                     AGVLRAGQLVVLESTTYPGTTRERLVPMLEESGLAAGVDFNVAFSPERIDPGRTDYTM
                     RNTPKVIGGLTPACLDRAVAVYGEVCDEIVPVSGPEPAELSKLLENVFRSVNIALVNE
                     LAMLCERMGIDVWEVVEAAATKPYGFMRFDPGPGMGGHCLPVDPFYLAWKAREYGQPT
                     EFVELAGEVNQRMPEFCVELVTAALNDHAKAVRGSRIAIIGASYKRDVGDLRESPALK
                     IMRMLDARGADLVYHDDFVPVLADFELENMPLDEALEGADCAVIVTAHSALDVPGVVA
                     GAPSVVDLRGVTRGIESPHVVRL"
     gene            complement(2084..>2571)
                     /locus_tag="VFY44_10090"
     CDS             complement(2084..>2571)
                     /locus_tag="VFY44_10090"
                     /inference="COORDINATES: protein motif:HMM:NF014411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEX5910834.1"
                     /translation="LATAHRAGNVDDPARLERLVALLEAVSGTVVLPLHPRTAGRLQE
                     FGLRGRLDAAEGVQLLPPLGYLDFMRLAMDARAVLTDSGGVQKEAYLLGVRCVTLRET
                     TEWVETVDAGWNTLVGLDRAAAVAALEAPAPAGERPELYGGGKAAGRIVEVLSAYTPG
                     P"
BASE COUNT          364 a          966 c          896 g          345 t
ORIGIN      
        1 gcgcgggctg taggtatcgc ccgagcgggt gatgtactcc ccgtaggagg tgctgtcctg
       61 gtcgaagccc ttgtcgtaca cggtcagctt gcgctccacg tccatgtcgt cgaacgtggc
      121 catcttcttc gacccgacga cggtgaagcg gcgctccttg tgcgggtcca gccaggacag
      181 gtgcaggtgc gcggccagcc ccgaggggaa gcgcaggaag cagaagacca cgtcctccac
      241 tcccgggcgc atgtaggact cgcccttggc gctgacctcg tacggctcct cgcccgccag
      301 gcgcagcagc accgacacgt cgtgggcgcc gaggctccag agcgcgttct cctcggcccg
      361 cagcttgccc aggttcaggc ggttgccgta gatgtagtgg atgtccccca gctcgccgtc
      421 ggtggccagg cggttcagca tctccagtcc ggggtggtac tccaggaggt gcccgaccat
      481 caggacccgg tccgcctcgg ccgccagcgc caccacccgc tcggcgtcgg ccaccgtctg
      541 ggccagcggc ttctcgacga agcagtgctt gcccgcggcc agcacccgct cggcgatcgc
      601 cgcgtgggtg ggcaccggcg tggccacgca caccgcgtcc agcgcggggt cggccagcag
      661 ctcgtccagg tcggcactct ggcgcgcgcc gggaaaggcc agcgcctggc gctcgcggcg
      721 ctcgtcgtcg gcgtcgcaga gccaggccag ctcgaccccg gggaggcggt cgaagttgcg
      781 ggccaggttg ggcccccagt agccgaggcc gacgaccccg accctcacgg gaccgtcgct
      841 caaagcctca ccacgtgcgg cgactcgatg ccccgcgtga ccccgcgcag gtccaccacc
      901 gacggcgcgc cggccacgac gcccggcacg tccaaggcgg aatgggcggt cacgatgacg
      961 gcgcagtcgg cgccctccag cgcctcgtcc agcggcatgt tctcgagctc gaagtccgcc
     1021 agcacgggca cgaagtcgtc gtggtagacc aggtcggccc cgcgggcgtc cagcatgcgc
     1081 atgatcttca gcgccggcga ctcgcgcagg tcgccgacgt cgcgcttgta ggaggcgccg
     1141 atgatggcga tccgcgaccc ccgcaccgcc ttggcgtggt cgttcagcgc cgccgtgacc
     1201 agctcgacgc agaactcggg catgcgctgg ttgacctcgc cggccagctc cacgaactcg
     1261 gtgggctggc cgtactcgcg cgccttccag gccaggtaga acgggtccac gggcaggcag
     1321 tgcccgccca tgccggggcc cgggtcgaag cgcatgaagc cgtacggctt ggtggccgcg
     1381 gcctcgacga cctcccacac gtcgatgccc atccgctcgc acagcatcgc cagctcgttg
     1441 accagggcga tgttcaccga gcggaagacg ttctccagga gcttggacag ctcggccggc
     1501 tcggggcccg atacgggcac gatctcgtcg cacacctcgc cgtacaccgc caccgcgcga
     1561 tccaggcacg ccggcgtcag gccgccgatg accttggggg tgttgcgcat cgtgtagtcg
     1621 gtgcggcccg ggtcgatccg ctcgggcgag aaggccacgt tgaagtcgac gccggcggcc
     1681 agccccgact cctcgagcat cggcaccagg cgctcacggg tcgtgcccgg gtaggtcgtc
     1741 gactccagca ccaccagctg cccggcgcgc aggacgcccg ccagcgaggt gccggccgag
     1801 atcagcggcc ccaggtcggg ctcgcggttg gtggtcaggg gcgtcggcac cgcgatcatc
     1861 accgcgtcgc agtcggccag ctccgcgtac cgggtgcccg cggtgatgcg gtcaccgacc
     1921 gcggcgagcc gctcggacga gacgtcctcg atgtagctct cgtgacgccc gagggcagcc
     1981 gcccgccggc tgtccgaatc cacgccggcc acggtgtggc cctcctcggc gaaggcgagg
     2041 gccagcggaa ggcccacgta cccgagccct atgacaccga tcttcatggc ccgggagtgt
     2101 aagccgagag cacttcgacg atccggcccg cggccttgcc gccgccgtag agctccgggc
     2161 gctccccggc cggggccggg gcctcgagcg cggccacggc ggccgcgcgg tccagcccga
     2221 ccagcgtgtt ccagcccgcg tccacggtct cgacccactc ggtggtctcg cgcagcgtca
     2281 cgcagcgcac gcccaggagg taggcctcct tctggacccc gcccgagtcg gtcagcacgg
     2341 cgcgggcgtc catggccagg cgcatgaagt ccaggtagcc cagcggcggc agcagctgaa
     2401 ccccctcggc cgcgtccagg cggccgcgca gcccgaactc ctggaggcgc ccggccgtcc
     2461 gcgggtgcag cggcagcacg acggtccccg agaccgcctc cagcagcgcc accagccgct
     2521 cgaggcgcgc gggatcgtcc acgttgcccg cccggtgggc cgtggccagg a
//
LOCUS       DASUPW010000447         2657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1626219, whole genome shotgun sequence.
ACCESSION   DASUPW010000447 DASUPW010000000
VERSION     DASUPW010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626219"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1817)
                     /gene="pknB"
                     /locus_tag="VFY44_10095"
     CDS             complement(<1..1817)
                     /gene="pknB"
                     /locus_tag="VFY44_10095"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEX5910835.1"
                     /translation="MSDVATDTVVDGRYKIVDRIGSGGMADVYRAEDTHLGREVALKI
                     LHRRFAQDAEFVERFRREARAAAGLQHPHVVGVYDRGEHEGTYYIAMELLEGRTLKDI
                     VTAEAPLDQLRVIELGRQVLQAAEFAHKRGVIHRDFKPHNVIVDAEGQAKVTDFGIAR
                     AGASEMTETGSIMGTAQYLSPEQAQGHAVTAASDVYSIGVMLYEMLTGKLPFEGDSAV
                     SIALKHLSDSPPPMAEHGVQVEPNLEAVVMGALAKEPASRWQSASDFADALEACKPYV
                     EAAQGGEPDNGTKDFAAVAAAPVPVNGNGDDEDEEKKRRWLPWLIGALVLALIAVMAY
                     AFTRPEETSVPKVEGLTLTKARDRLERDGFEKVKVERERSNAEVDTVLRQDPDAGEAA
                     AASETITLIVSRGPGNVRVPSVRNTSRQLAIRELQKQGLEVTADTEPSGAVRKGFATR
                     TSPGEGTSVERGSRVRLFVSSGPARVEVPDVVGLTREAAETRLTRARLDVNVELRDSD
                     APENEVIRQSPSGGSSAKRGDEVTIVVSRGEQQTEVPNVTGLDLADARRVLRGEGLGV
                     TVRRRTTGNRADDGQVLEQRPSSGQDVDEGASVIVVVGE"
     gene            complement(1814..>2657)
                     /locus_tag="VFY44_10100"
     CDS             complement(1814..>2657)
                     /locus_tag="VFY44_10100"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX5910836.1"
                     /translation="SLYPPGSTFKVVTAAAALDSGKYTPQSVVDGSSPRDVSGVPLAN
                     SGGQDFGPISLTDALTNSVNTVWAQVGESIGRGTLEEYMGRFGFNKDPQLDYPDGQMA
                     PSGVRGDKGQLLTADAGWDVGRVAIGQGGAEGAILATPLQMAQVAGAVGNKGRLMRPR
                     LTEKIVAKDGRAEDIRPVQQSRVMSEKAAGELTQMMGNVVEEGTGTAAALSGAKVAGK
                     TGTAEVDNATANQAWFIGFAPLDNPKMAIAVTIERTQGQGGTEAAPLAKQVLEALLGP
                     GGVR"
BASE COUNT          307 a         1014 c          921 g          415 t
ORIGIN      
        1 tactcgccca cgacgacgat caccgacgca ccctcgtcca cgtcctggcc ggacgacggg
       61 cgctgttcca gcacctggcc gtcgtcggcg cggttgccgg tcgtgcggcg ccggacggtc
      121 acgcccagcc cctcgccgcg cagcacgcgg cgcgcgtcgg ccaggtcgag ccccgtcacg
      181 ttggggacct cggtctgctg ctcgccgcgc gagaccacga tggtcacctc gtcgccgcgc
      241 ttggcgctcg acccgcccga ggggctctgg cggatgacct cgttctcggg ggcgtccgag
      301 tcgcgcagct ccacgttcac gtccagccgg gcgcgcgtga gccgcgtctc ggccgcctcc
      361 ctggtcaggc cgaccacgtc cggcacctcg accctggccg ggccggagct gacgaacaac
      421 cgcacgcgcg agccccgctc gaccgacgtg ccctcgccgg gcgacgtgcg ggtggcgaag
      481 cccttgcgca ccgcaccgga gggctcggtg tcggcggtga cctccaggcc ctgtttctgg
      541 agctcgcgta tggccagctg gcgcgaggtg ttgcgcaccg agggcacgcg gacgttgccc
      601 gggccgcggg agacgatcag ggtgatggtc tccgacgccg cggcggcctc gcccgcatcg
      661 gggtcctggc gcagcacggt gtccacctcg gcgttgctgc gctcgcgctc gaccttgacc
      721 ttctcgaacc cgtcgcgctc gagccggtcg cgggccttcg tcagcgtgag cccctcgacc
      781 ttgggcaccg acgtctcctc gggccgcgtg aaggcgtagg ccatgacggc gatcagggcc
      841 aggaccagcg cgccgatcag ccacggcagc cagcggcgct tcttctcctc gtcctcgtcg
      901 tcgccgttcc cgttcaccgg caccggagcg gcggccacgg cggcgaagtc cttggtgccg
      961 ttgtcgggct cgccgccctg agccgcctcg acgtagggct tgcacgcctc gagcgcgtcc
     1021 gcgaagtccg acgcgctctg ccaccgcgac gcgggctcct tggccagcgc gcccatgacc
     1081 acggcctcga ggttgggctc gacctgcacg ccgtgctcgg ccatgggcgg cgggctgtcg
     1141 gacaggtgct tcagggcgat ggacaccgcg ctgtcgccct cgaacggcag cttgccggtg
     1201 agcatctcgt agagcatcac gccgatcgag tagacgtccg acgcggcggt cacggcgtgg
     1261 ccctgggcct gctcgggcga gaggtactgg gcggtgccca tgatcgagcc ggtctcggtc
     1321 atctccgacg cgccggcgcg cgcgatgccg aagtccgtga ccttggcctg cccctcggcg
     1381 tccacgatca cgttgtgggg cttgaagtcg cggtggatga cgccgcgctt gtgggcgaac
     1441 tccgcggcct gcaggacctg gcgtcccagc tcgatcacgc gcagctggtc cagcggcgcc
     1501 tcggcggtca cgatgtcctt gagcgtgcgc ccctcgagca gctccatggc gatgtagtag
     1561 gtgccctcgt gctcaccgcg gtcgtacacg ccgaccacgt gcgggtgctg gaggccggcc
     1621 gcggcgcgcg cctcgcgccg gaaccgctcc acgaactcgg cgtcctgggc gaagcgccgg
     1681 tgcaggatct tcagcgcgac ctcgcggccc aggtgggtgt cctcggcgcg gtagacgtcg
     1741 gccatcccgc cggagccgat ccggtcgacg atcttgtagc gcccgtccac cacggtgtcg
     1801 gtggccacgt cgctcaccgc acgcctccgg ggcccagcag cgcctcgagc acctgcttgg
     1861 ccagcggcgc cgcctcggtg ccgccctggc cctgggtgcg ctcgatggtc acggcgatcg
     1921 ccatcttggg gttgtccagc ggcgcgaagc cgatgaacca ggcctggttg gccgtcgcgt
     1981 tgtccacctc ggccgtgccg gtcttgccgg ccaccttggc gcccgacagc gccgcggccg
     2041 tgccggttcc ctcctcgacc acgttgccca tcatctgggt cagctcgccg gccgccttct
     2101 ccgacatcac gcgggactgc tgcaccgggc ggatgtcctc ggcgcgcccg tccttggcca
     2161 cgatcttctc ggtcaggcgc ggccgcatca gccggccctt gttgcccacc gcgccggcca
     2221 cctgggccat ctgcagcggc gtggccagga tcgcgccctc ggcaccgccc tggccgatgg
     2281 ccacgcggcc cacgtcccag ccggcgtccg cggtcagcag ctggcccttg tcgccgcgca
     2341 cgccgctggg cgccatctgg ccgtcggggt agtccagctg cgggtccttg ttgaacccga
     2401 accggcccat gtactcctcc agcgtcccgc gcccgatgct ctcccccacc tgcgcccaga
     2461 ccgtgttgac cgagttggtc agcgcgtcgg tcagcgagat cgggccgaag tcctggccgc
     2521 cggagttggc cagcggcacc ccgctgacgt cccgcggcga cgagccgtcg accacggact
     2581 gcggggtgta cttgccgctg tccagcgccg ccgcggcggt gaccaccttg aacgtggacc
     2641 ccggcgggta gagcgac
//
LOCUS       DASUPW010000448         3193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_381308, whole genome shotgun sequence.
ACCESSION   DASUPW010000448 DASUPW010000000
VERSION     DASUPW010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381308"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VFY44_10105"
     CDS             <1..388
                     /locus_tag="VFY44_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910837.1"
                     /translation="AAAALLALAVSAATERVPALERQARTAQRTAELDRDLGVAVARM
                     GGADAVVRRGAPAVDRAFIPRMAWLTTLPIGQVERRSGRPGLVFAAGRRDPVTLVAQT
                     TPAPALSGRLVVRRWRVYERRVSRSR"
     gene            complement(372..746)
                     /locus_tag="VFY44_10110"
     CDS             complement(372..746)
                     /locus_tag="VFY44_10110"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5910838.1"
                     /translation="MSELIHTCYRIGDIDKSVAFYEKLGFEEIRRMPIRDEAINVFMG
                     LPDDGARLELTYNHGVDSYEMGTGYNHIAVTVEDLDGTLEGLAGQGIEPEKPPSQVRE
                     GGNRICFVRDPDGYRIELIETG"
     gene            781..1083
                     /locus_tag="VFY44_10115"
     CDS             781..1083
                     /locus_tag="VFY44_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910839.1"
                     /translation="MDPAALRNRLVMATSMWREYTDEPLPKMPPGDPEEQISGFELKL
                     VEMVFAQATASTARRAADQTWDIVHDRPDTDPVKVRVVAGHEHLARLAAEGGDQPG"
     gene            1096..>3193
                     /locus_tag="VFY44_10120"
     CDS             1096..>3193
                     /locus_tag="VFY44_10120"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX5910840.1"
                     /translation="MGAFYSQRVKARTLGFLIIGVGLAGLLLVSMPHSEMVKTEAAWV
                     FVPIAVAIGAAVVLLAGRIDATGLRVAVVAGIVVVSVANYVLGPVALVPIMFTWFALY
                     VFAFFGRKEAMAYMAVMALAFALVLTEQDVTSPVVRWLLGTVTPVVGGLLLSMVVRLA
                     MDRTGVLEDSEAQTRAIIESAPNAFTTVDQDGVIMEWNREAERTFGYTAEEVVGRQVA
                     DVIILPEDREGHFQRLHAAFATSPDDPPLRGERNLMRKDGTSLFCEVTLSLVAFDEDH
                     ILLVFMRDLSHRAERERQREQLFREQAARQEAEHMAEMVQGLQMLLDAALAHGRLEQI
                     LELLVPRLCEVLSAEAAAIFLTEEGTGELVLRASTDGAPEERVVARDQHVAARVADSR
                     APLLVHDPPDDNLSASAMRGMGSVISVPLTAGEEVTGVIQVGVPPPRRFDDDNLLLLG
                     LAADRVALAIDHARIFEREHRIAETLQRSLLPDRLPVLPGLEVAARYIPAASEAEVGG
                     DWYDVISIDASRVGLVMGDVAGKGLAAASMVGRLGSAMRAYALEGREPAEVVASLNQL
                     VWSEVGDSEMATLIYVVVDPVAETISWVNAGHPPPLILAADGSTSFLEGPRSVPLGVM
                     PFPSYEAGTAPLPEGGTLLLYTDGLVERPGELLDDGFARLAVAAEGADVSAERACDHV
                     LERLVPTGAMADDVALL"
BASE COUNT          396 a         1110 c         1211 g          476 t
ORIGIN      
        1 cgcggcggcg gccctgctgg cgctcgcggt ctccgcggcg accgagcggg tccccgcgct
       61 ggagcgccag gcgcggaccg cgcagcgcac cgcggagctg gaccgcgacc tgggcgtggc
      121 ggtcgcgcgc atgggtgggg ccgatgcggt ggtcaggcgc ggcgcgcccg cggtcgaccg
      181 ggcgttcatc ccgcggatgg cctggttgac cacgctgccc atcgggcagg tcgagcgccg
      241 ctcgggccgc cccggcctgg tgttcgccgc ggggcggcgc gacccggtca cgctggtcgc
      301 gcagaccacg ccggcgcccg cgctgtccgg gcggctggtg gtccggcgct ggagggtcta
      361 cgagcggcgg gtcagccggt ctcgatgagc tcgatccggt acccgtccgg atcgcgcacg
      421 aagcagatgc ggttgccgcc ctcgcgcacc tgggacggcg gcttctcggg ctcgatgcct
      481 tgcccggcga gcccctcgag cgtcccgtcc aggtcctcga cggtcaccgc gatgtggttg
      541 tagccggtgc ccatctcgta ggagtcgacg ccgtggttgt aggtcagctc cagccgcgcg
      601 ccgtcgtcgg ggaggcccat gaacacgttg atggcctcgt cgcggatggg cattcgcctg
      661 atctcctcga agcccagctt ctcgtagaag gccaccgact tgtcgatgtc gccgatgcgg
      721 taacaggtgt ggatcagctc gctcacgccc cggacgttag tacgtactag cctcccccgg
      781 atggatcccg ctgccctgcg caaccggctg gtcatggcca cgagcatgtg gcgtgagtac
      841 accgacgagc cgctgcccaa gatgcccccc ggcgaccccg aggaacagat cagcgggttc
      901 gagctgaagc tggtcgagat ggtgttcgcc caggccaccg cgtcgaccgc ccgccgggcg
      961 gccgaccaga cctgggacat cgtgcacgac cgccccgaca ccgatccggt gaaggtgcgg
     1021 gtggtggccg gccacgagca cctggcgcgc ctggccgccg agggcggcga ccagccggga
     1081 tagtctgacc ccccgatggg ggcgttctac agccagcggg tcaaggcccg caccctgggc
     1141 ttcctgatca tcggcgtcgg gctggccggc ctgctgctgg tctccatgcc gcactccgag
     1201 atggtcaaga cggaggccgc gtgggtgttc gtgcccatcg cggtcgcgat cggcgcggcg
     1261 gtggtgcttc tggccgggcg gatcgacgcg accggcctgc gcgtcgcggt ggtggcgggc
     1321 atcgtcgtgg tcagcgtggc caactacgtg ctcggcccgg tggcgctggt gccgatcatg
     1381 ttcacctggt tcgcgcttta cgtgttcgcg ttcttcggcc gcaaggaggc gatggcctac
     1441 atggccgtca tggccctggc cttcgcgctc gtgctcaccg agcaggacgt gaccagcccg
     1501 gtcgtgcgct ggctgctggg cacggtcacg ccggtggtcg gtgggctgct cctgtcgatg
     1561 gtggtgcgcc tggccatgga ccggaccggg gtgctcgagg acagcgaggc gcagacccgg
     1621 gccatcatcg agtccgcgcc caacgcgttc acgaccgtcg accaggacgg ggtgatcatg
     1681 gagtggaacc gcgaggcgga gcgcacgttc ggctacaccg ccgaggaggt cgtgggccgg
     1741 caggtggccg acgtgatcat cctgcccgag gaccgggagg ggcacttcca gcggctgcac
     1801 gcggcgttcg cgacctcgcc cgacgacccg ccgctgcgcg gcgagcgcaa cctcatgcgc
     1861 aaggacggca cgtcgctgtt ctgcgaggtc acgctgagcc tcgtcgcgtt cgacgaggac
     1921 cacatcctcc tggtgttcat gcgcgacctg agccaccgcg cggagcgcga gcgccagcgc
     1981 gagcagctct tccgcgagca ggcggcccgc caggaggccg agcacatggc cgagatggtc
     2041 cagggcctgc agatgctcct ggacgccgcg ctggcccacg gacggctgga gcagatactg
     2101 gagctgctgg tcccgcggct gtgcgaggtg ctgtcggccg aggcggcggc catcttcctg
     2161 accgaggagg gaacgggcga gctcgtgctg cgcgcgtcca ccgacggggc cccggaggag
     2221 cgggtcgtgg cccgtgacca gcacgtggcc gcgcgcgtgg ccgactcgcg cgcgccgctg
     2281 ctggtgcacg accccccgga cgacaacctg tcggcctccg cgatgcgggg catgggctcg
     2341 gtgatctcgg tgccgctgac cgcgggcgag gaggtgaccg gcgtgatcca ggtgggcgtg
     2401 ccgccgccgc gccgcttcga cgacgacaac ctgctgctgc tgggcctggc cgccgaccgc
     2461 gtggcgctgg ccatcgacca cgcgcgcatc ttcgagcgcg agcaccggat cgccgagacg
     2521 ctccagcgca gcctgttgcc cgaccgcctg ccggtgctgc cgggcctgga ggtcgcggcg
     2581 cgctacatcc cggccgccag cgaggccgag gtgggcggcg actggtacga cgtgatctcc
     2641 atcgacgcgt cccgcgtggg cctggtcatg ggcgacgtgg caggcaaggg cctggccgcc
     2701 gcgtccatgg tcggccggct gggctcggcc atgcgcgcgt acgcgctgga ggggcgggag
     2761 ccggcggagg tggtcgccag cctgaaccag ctcgtctggt ccgaggtggg cgacagcgag
     2821 atggcgacgc tgatctacgt ggtggtcgac cccgtggccg agaccatctc ctgggtcaac
     2881 gccgggcatc cgccgcccct gatcctggct gccgacggct ccacctcctt cctcgagggc
     2941 ccgcggtcgg tcccgctggg cgtgatgccg ttcccgtcct acgaggcggg caccgcaccg
     3001 ctccccgagg gcggcacgct gctgctgtac accgacgggc tggtcgagcg gccgggggag
     3061 ctgctggacg acggcttcgc ccggctggcg gtggcggccg agggtgccga cgtctcggcc
     3121 gagcgggcgt gcgaccacgt gctggagcgc ctggtgccga cgggcgcgat ggcggacgac
     3181 gttgcgctcc ttg
//
LOCUS       DASUPW010000449         5251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_656607, whole genome shotgun sequence.
ACCESSION   DASUPW010000449 DASUPW010000000
VERSION     DASUPW010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656607"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1549)
                     /locus_tag="VFY44_10125"
     CDS             complement(<1..1549)
                     /locus_tag="VFY44_10125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5910841.1"
                     /translation="MSSRAARLALFVGAALISGFTILRGGAPFDEGLVLQAARRVADG
                     EVPYRDFQWAYGPADAYLLGGWFKAFGVSLLSWRILRVVCDAVVAVLVYELVRRGAPR
                     WIALVAWLATACAMAQPTSASPGAPTLVFAFAGLAVATRTPPDRRAPALAGVLVGIAL
                     LWRLDFGLYAAGAVGIALLVTSQPLRERLRAVGVFSACTTAVALIGYLPFIVAAGPAD
                     FYEAILGRSLTERDYWTLPFPLSYDGSVGVWPPGQALDDAKDLVNYYVPALLVAGLVL
                     VGAALAVIAWRRRRLDPAAAGLITFAVGGLLYLYSRADEFHTAPMLVALTAALGLAWA
                     ALRAPEFGRAGRAAAAGLLALLVLLAGTGLANRLSALLRPPALAAIDLPGTQGAKAPP
                     AEARALRRTVRAVQARVPPDQPIYTVTRRSDLVRFNQPVLYVLAQRDNATAVDFGLQT
                     SPRAQRRLVAQLRRARPRVVVRWLDPITTVREPNLRGKPSGSRALDEYLARAYRPGPR
                     YGQYRLLL"
     gene            complement(1546..2766)
                     /locus_tag="VFY44_10130"
     CDS             complement(1546..2766)
                     /locus_tag="VFY44_10130"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX5910842.1"
                     /translation="MNARAERFPLFDSLRAIAALSVIGVHGAVTAGIYNDSTLRQFVS
                     HLDVGVQIFFVISGFLLYRPFARARLEGRSRPATGPYAWRRFLRIVPAYWVALTVIAW
                     VQLDHAEVFTAIDIPRYYGFLQVYDPGTPFKGIPQAWTLCVEVAFYAVLPLWALLMRR
                     VPARSPRSWVRSELIMLAGLFAASGLYKAFMVKQGAITDPSFGPYMLSVPAFLDQFAI
                     GMALAVLSVWFADPGHSRPRLLGAVERWPVLPWAFAAAAFVMVSVGTGYDVNGGTPLT
                     PFQFFAEHYLFAAVALGVVLPGVFGDPEQGVVRRILANRVLLSLGVVSYGMYLWHSAA
                     FEQLNRWGFGTVAAETHPYAWYAAGIAVTVVVATASYFVVEKPALRLKRLVGSRDPGP
                     PGEALREPTAVGGR"
     gene            2828..3526
                     /locus_tag="VFY44_10135"
     CDS             2828..3526
                     /locus_tag="VFY44_10135"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5910843.1"
                     /translation="MPDLTIVMPVYNELNTVTDAVERVLAIEYPVDSVELLIVDDGST
                     DGTRALLDDGDWPEQVRIVKHDRNRGKGAALRTGFKEARGTWSAIMDADLEYDPADLP
                     KLLEPLLNGDATVVFGTRGFESHSAFSFWYVVGNKAVTFAASILFDAWLADIMTCHKL
                     MRTELFRSLPLRANGFAVEAEITAGVLNAGHRVFEVPIEYHARGRDAGKKLTSTDGLR
                     VIGSLLRCRLAPAR"
     gene            3546..4736
                     /locus_tag="VFY44_10140"
     CDS             3546..4736
                     /locus_tag="VFY44_10140"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX5910844.1"
                     /translation="MSATRGTHAATHVDPVVAPPPGEKRFPLIDSMRGIAALCVLVTH
                     AAAVSGETIESPAGKVWAQLTVGVPIFFLVSGFLLYRPYFSARYHGYAAPSARTFFRR
                     RILRIVPAYWAALLVLAIGLTLSGVLTSDWWVYFGFLQAYQRDTVLQGIAPAWSLCVE
                     VTFYLVLPMYAVLAARFSRRMAKARMVAVDLAVLGALGLASVAIRTWAADADETLWFQ
                     TLPTTFDWFALGMALAVLSVAYDRRALPRPLQALAARPGACWLGAIAAFLAMCLLLDL
                     PLTLVGRYTPAQVLVQHLTFGLVALLLMLPAVFDDDAGGVPRRLLAWPTLAWLGTISY
                     GIFLYNLTLCEWLQGRGVADWLPGGRFPPLVLVTFLASVAAAAASYYALERPILRLKY
                     RRRT"
     gene            4733..5143
                     /locus_tag="VFY44_10145"
     CDS             4733..5143
                     /locus_tag="VFY44_10145"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5910845.1"
                     /translation="MKARIALAVASVLVLGVLGFWLRASTLESRAYDVSLNAFQVTKP
                     GPLAQARSDYRRAERHNPDTRPLLLRGDLLFKARRYREAAAVLRELVREEPQNVDGWA
                     LLAASSEPFDRAAADRARARLRALNPLGLGNSGG"
BASE COUNT          726 a         1915 c         1941 g          669 t
ORIGIN      
        1 tcagcagcag gcggtactgc ccgtaccggg ggccgggccg gtaggcccgc gccaggtact
       61 cgtcgagggc ccgcgagccg ctcggcttcc cgcgcaggtt gggctcgcgc acggtggtga
      121 tcgggtccag ccagcgcacc accacgcgcg gccgggcacg gcgcagctgc gccaccaggc
      181 ggcgctgggc ccgggggctg gtctggaggc cgaagtcgac cgccgtggcg ttgtcgcgct
      241 gggccagcac gtacagcacc ggctggttga agcgcaccag gtcggagcgc cgggtcacgg
      301 tgtagatggg ctgatcgggc ggcacgcggg cctggaccgc ccgcacggtc cggcgcagcg
      361 cccgggcctc ggcgggggga gccttggcgc cctgcgtgcc ggggaggtcg atggcggcca
      421 gcgcgggtgg ccgcagcagc gcggacaggc ggttggccag cccggtcccg gccagcagca
      481 ccagcagggc cagcagaccg gcggcggcgg cccgtcccgc gcggccgaac tcgggtgcgc
      541 gcagggccgc ccaggccagg cccagcgccg ccgtcagcgc cacgagcatg ggggcggtgt
      601 gaaactcgtc cgcgcgcgag tagaggtaca gcaggccgcc caccgcgaac gtgatcagcc
      661 ctgcggcggc cgggtcgagc cggcgccggc gccacgcgat gacggccagg gcggcgccca
      721 cgagcaccag cccggccacc aggagcgcgg gcacgtagta gttcaccagg tccttggcgt
      781 cgtccagcgc ctgccccggg ggccacacgc ccacgctccc gtcgtagctc agcgggaacg
      841 gcagcgtcca gtagtcgcgc tcggtcagcg accggcccag gatggcctcg tagaagtcgg
      901 ccggccccgc ggccacgatg aacggcaggt acccgatcag cgccacggcg gtggtgcacg
      961 cgctgaacac ccctaccgcg cgcagccgct cccgcagcgg ctgcgaggtc accagcaacg
     1021 cgatgccgac ggctcccgcc gcgtacaggc cgaagtccag ccgccacagc agcgcgatgc
     1081 ccaccagcac ccccgccagc gcgggggcgc gccggtccgg tggcgtgcgg gtggccaccg
     1141 ccagcccggc gaaggcgaac accagcgtgg gcgccccggg gctggcgctg gtgggctggg
     1201 ccatggcgca cgccgtggcc agccaggcca cgagggcgat ccacctgggc gcgccccggc
     1261 ggaccagctc gtacaccagc acggccacga cggcgtcgca gaccacccgg aggatccgcc
     1321 agctcagcag cgatacgccg aaggccttga accatccgcc cagcaggtag gcgtcggcgg
     1381 gcccgtaggc ccactggaag tcgcggtagg gcacctcgcc gtccgcgacc cgccgtgccg
     1441 cctgcagcac caggccctcg tcgaacggcg cgcctccgcg caggatcgtg aagcccgaga
     1501 tcagcgccgc gcccacgaac agtgcgagcc gggctgcgcg gctgctcacc gcccgcccac
     1561 cgcggtcggc tcgcgcagcg cctcgccggg cggccccggg tcacgggagc ccaccagccg
     1621 cttgagcctg agcgccggct tctcgaccac gaagtagctg gcggtggcca cgaccacggt
     1681 gaccgcgatg ccggccgcgt accaggcgta cgggtgggtc tcggcggcca ccgtgccgaa
     1741 gccccagcgg ttgagctgct cgaacgccgc gctgtgccac aggtacatgc cgtaggacac
     1801 gacgcccagc gacagcagca cccggttggc caggatccgc cgcaccacgc cctgctcggg
     1861 gtcaccgaac acgcccggga gcaccacgcc cagcgccacc gcggcgaaca ggtagtgctc
     1921 ggcgaagaac tggaacggcg tcagcggcgt gcccccgttg acgtcgtagc cggtgcccac
     1981 gctgaccatc acgaacgccg ccgccgcgaa cgcccagggc agcaccggcc accgctcgac
     2041 ggcgcccagc aggcgcgggc gcgagtggcc ggggtcggcg aaccacacgc tcagcacggc
     2101 cagggccatc ccgatcgcga actggtccag gaaggccggc accgacagca tgtacgggcc
     2161 gaacgagggg tcggtgatgg cgccctgctt gaccatgaac gccttgtaga gcccgctggc
     2221 ggcgaacagg ccggccagca tgatcagctc ggaccgcacc cacgagcgtg gcgagcgggc
     2281 ggggacgcgc cgcatcagca gcgcccacag cggcagcacc gcgtagaagg ccacctcgac
     2341 gcacagggtc caggcctgcg ggatgccctt gaacggggtg ccggggtcgt agacctggag
     2401 gaagccgtag tagcgcggga tgtcgatggc ggtgaacacc tcggcgtggt ccagctggac
     2461 ccaggcgatc acggtcagcg ccacccagta ggccggcacg atgcgcagga agcggcgcca
     2521 cgcgtagggc ccggtggccg gccggctgcg tccctccagc cgggcgcggg cgaacggccg
     2581 gtagagcagg aaccccgaga tcacgaagaa gatctggacc ccgacgtcca ggtgcgacac
     2641 gaactggcgc agcgtcgagt cgttgtagat cccggccgtg acggcgccgt ggacgccgat
     2701 caccgacagg gcggcgatcg cgcgcaggga gtcgaacagg gggaagcgtt ccgcgcgtgc
     2761 gttcaagggg ccggaggcta cccgtcggat cccggatccc cggcgccgga cgctagggtg
     2821 gcgccccatg ccagacctga ccatcgtgat gcccgtctac aacgagctca acaccgtgac
     2881 cgacgcggtc gagcgcgttc tggcgatcga gtaccccgtg gactcggtgg agctgctgat
     2941 cgtggacgac gggtcgaccg acgggacgcg cgccctgctc gacgacggcg actggcccga
     3001 gcaggtgcgg atcgtcaagc acgaccgcaa ccgcggcaag ggcgccgccc tgcggacggg
     3061 cttcaaggag gcccggggga cgtggtcggc catcatggac gccgacctgg agtacgaccc
     3121 ggccgacctg cccaagctgc tggagccgct gctcaacggc gacgccaccg tggtgttcgg
     3181 cacccgcggc ttcgagtcgc actcggcctt cagcttctgg tacgtcgtgg gcaacaaggc
     3241 cgtgacgttc gcggccagca tcctcttcga cgcgtggctg gcggacatca tgacctgcca
     3301 caagctgatg cggacggagc tgttccggtc gctgccgctg cgcgccaacg gcttcgccgt
     3361 cgaggccgag atcaccgcgg gcgtgctcaa cgccggccac cgggtgttcg aggtgccgat
     3421 cgagtaccac gcccgtggcc gcgacgcggg caagaagctg acctcgaccg acgggctccg
     3481 ggtcatcggc tcgctgctgc gctgccgctt ggctccggcg cgctaggcgc cacccctcct
     3541 ggcccgtgag cgccacacgg ggcacacacg ccgccacgca cgtggacccg gtcgtggctc
     3601 ccccgccggg ggagaagcgg ttcccgctga tcgactcgat gcgcgggatc gcggccctgt
     3661 gcgtgctggt gacccatgcc gcggccgtct ccggcgagac catcgagagc ccggccggca
     3721 aggtgtgggc ccagctcacg gtcggggtgc cgatcttctt cctggtctcc gggttcctgc
     3781 tgtaccggcc ctacttctcg gcccggtacc acggctacgc ggccccgagc gcgcgcacgt
     3841 tctttcgccg ccggatcctg cgcatcgtgc ccgcctactg ggccgcgctg ctggtgctgg
     3901 ccatcggcct gaccctgagc ggcgtgctga ccagcgactg gtgggtctac ttcggcttcc
     3961 tccaggccta ccagcgcgac acggtgctgc agggcatcgc gcccgcgtgg agcctgtgcg
     4021 tggaggtcac cttctacctg gtgctgccga tgtacgcggt gctggccgcc cggttctcgc
     4081 gccggatggc caaggcgcgg atggtggcgg tggacctggc ggtgctcggc gcgctgggcc
     4141 tcgcctccgt cgcgatccgg acgtgggcgg cggacgccga cgagaccctg tggttccaga
     4201 cgctgccgac caccttcgac tggttcgcgc tgggcatggc gctggccgtg ctcagcgtgg
     4261 cctacgacag gcgcgcgctg ccgcggccgc tgcaggcgct ggccgcgcgt cccggcgcgt
     4321 gctggctggg ggcgatcgcg gcgttcctgg ccatgtgcct gctgctggac ctgccgctga
     4381 cgctggtggg ccgctacacg ccggcccagg tgctggtcca gcacctgacc ttcggcctgg
     4441 tggcgctgct gctgatgctg cccgcggtgt tcgacgacga cgccgggggc gtgccccggc
     4501 gcctgctggc ctggccgacg ctggcgtggc tgggcacgat ctcctacggc atcttcctct
     4561 acaacctgac gctgtgcgag tggttgcagg gacgcggcgt ggctgactgg ctgcctggag
     4621 ggcggttccc gccgctggtg ctggtcacgt tcctggcctc ggtggccgcg gcggcggcaa
     4681 gctactacgc gctggagcgg ccgatcctgc gcctgaagta ccggaggcgg acatgaaggc
     4741 gcggatcgcg ctggccgtgg cctcggtgct ggtgctgggc gtgctggggt tctggctccg
     4801 cgcctccacc ctggagtcgc gcgcctacga cgtgtcgctg aacgccttcc aggtgaccaa
     4861 gcccgggccg ctggcccagg cccgcagcga ctaccggcgg gccgagcgcc acaaccccga
     4921 cacccgcccg ctgctgctgc gcggcgacct gctgttcaag gccaggcgct accgggaggc
     4981 cgccgcggtc ctgcgcgagc tggtgcggga ggagccccag aacgtggacg gctgggcgct
     5041 gctggcggcg tcgtcggagc cgttcgaccg cgccgccgcc gaccgggcgc gggcccgcct
     5101 gcgcgcgctg aacccgctgg gcctgggcaa cagcggcgga tgaccagcct gcctccctca
     5161 cccgcccacc tgcggcggga ggcgctggtg gcgctggtcg gcgggctggc gctggcctgc
     5221 gtgatgttct ggccgctgcc gctgcatctg a
//
LOCUS       DASUPW010000450         2342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_795838, whole genome shotgun sequence.
ACCESSION   DASUPW010000450 DASUPW010000000
VERSION     DASUPW010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795838"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..640
                     /locus_tag="VFY44_10150"
     CDS             131..640
                     /locus_tag="VFY44_10150"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5910846.1"
                     /translation="MLTRLRGTPLPAPAFLAGRVATAVGMAVALTVVLLVIGRVAYGV
                     ELPGSTAPGVVLSLVVGAAAFCSLGVAFSTLVPNEDAGPAVVNAAVLPLYFISGVFFP
                     TDEAPGWLNDVASVFPVKHLAEALLEAFEPSSPAPGIAWGHLGIVALWGVAGALAAVF
                     TFRWTPKGE"
     gene            complement(645..1445)
                     /locus_tag="VFY44_10155"
     CDS             complement(645..1445)
                     /locus_tag="VFY44_10155"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX5910847.1"
                     /translation="MPEGDTIHYAARRVGAALTGREIRSIETPHPRTRGERWTQKLDG
                     RGVRSVDAHGKHLFIRFEGDLTLHSHLRMTGKWGVYRHGERWRRSRRRAWLVIRTEEH
                     EVVQFDGPVLQLTTDSRTRFDPHLAGLGPDVLAPEFDQDKFLERIREDDQTRGLGEAL
                     LDQRNLAGIGNIWKAESCFMAGLDPWKPTTRVTDDQLLQLVENIRPLMAKSAERGGHI
                     TTFEGDRRGATWVYGRDGLPCRRCGTLVKAHGQGDDNRTTFWCPGCQT"
     gene            1526..1933
                     /locus_tag="VFY44_10160"
     CDS             1526..1933
                     /locus_tag="VFY44_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910848.1"
                     /translation="MRRAAALAATIATLALAPAAGAESWELAGPQWVQVNECAGTTVG
                     VRASQPGDALGRPMSTRFSYQWQNAAGAWLPVPGAGSPWLAAGPGPWLNRETGWTQTF
                     LSGAGTRVRGVVQMRWGSAAGRTLVTPGSCLLR"
     gene            1933..>2342
                     /locus_tag="VFY44_10165"
     CDS             1933..>2342
                     /locus_tag="VFY44_10165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5910849.1"
                     /translation="MHIRRAGLADADAVLAVWEAARSAGADRPDDLAEYALLLERSPD
                     GVLVAEVDGRVVGTVIAVWDGWRGAIARLCVLPSHRRRGIGHALVAAGEEELRAKGVR
                     RVNVLSTRRTPAPATCGPRADTPPTRASRATGATS"
BASE COUNT          258 a          850 c          897 g          337 t
ORIGIN      
        1 gtcgttgccg aacacggtgg cgaagatgac caggaacagc accggcatca gcagcccgaa
       61 gaacaccgcg ggcgggttgc gcaggtagga gatcgtggcg tagcggacct ggcgcgcggc
      121 cagcttcgcc gtgctcacgc ggctgcgcgg gacgccgctg ccggcgcccg cgttcctggc
      181 cggccgggtg gccaccgcgg tcggcatggc cgtggcgctg acggtggtgc tgctggtgat
      241 cgggcgcgtg gcctacgggg tcgagctgcc ggggtcgacg gcgcccggcg tggtgctgtc
      301 gctggtggtc ggcgcggcgg cgttctgctc gctgggcgtg gcgttctcga cgctggtgcc
      361 caacgaggac gccggcccgg cggtggtgaa cgcggccgtg ctgccgctgt acttcatctc
      421 gggcgtgttc ttccccaccg acgaggcgcc gggctggctc aacgacgtgg cctcggtgtt
      481 cccggtcaag cacctggccg aggcgctgct ggaggcgttc gagccctcct cgccggcccc
      541 gggcatcgcc tggggccacc tcgggatcgt ggcgctgtgg ggcgtcgcgg gcgcgctggc
      601 cgcggtgttc acgttccgct ggaccccgaa gggggagtag ccgctcaggt ctggcagccc
      661 gggcaccaga aggtggtccg gttgtcgtca ccctggccgt gcgccttcac cagcgtgccg
      721 cagcgccggc acgggagccc gtcgcgcccg tagacccagg tggcgccgcg ccggtcgccc
      781 tcgaaggtcg tgatgtggcc gccgcgctcg gccgacttgg ccatcagcgg ccggatgttc
      841 tcgaccagct ggagcagctg gtcgtcggtc acccgcgtgg tcggcttcca cgggtcgagc
      901 ccggccatga agcacgactc ggccttccag atgttgccga tgccggccag gttgcgctgg
      961 tccagcagcg cctcgccgag tccgcgcgtc tggtcgtcct cgcggatgcg ctccaggaac
     1021 ttgtcctggt cgaactccgg cgccagcacg tcggggccca ggccggccag gtgcgggtcg
     1081 aagcgggtgc gcgagtcggt ggtcagctgc agcaccgggc cgtcgaactg gaccacctcg
     1141 tgctcctcgg tgcggatgac cagccacgcg cgccggcgcg acctacgcca gcgctcgccg
     1201 tggcggtaca cgccccactt cccggtcatg cgcaggtgcg agtgcagcgt caggtcgccc
     1261 tcgaaccgga tgaacaggtg cttgccgtgg gcgtcaaccg accgcacccc gcggccgtcc
     1321 agcttctggg tccagcgctc gccgcgcgtg cgcgggtgcg gcgtctcgat cgagcggatc
     1381 tcgcgcccgg tcagcgctgc gccgacgcgc ctggctgcgt agtggatcgt gtcgccctcg
     1441 ggcatgtgtc gatcctaggc ggcggccacc gccgtgccgg gctactctgg ggcggggagg
     1501 cgccggctcg aaccggaagg gccgcatgcg ccgcgcagca gcacttgcag caaccatcgc
     1561 cacgctggcc ctggcgcccg ccgccggggc cgagtcctgg gagctcgcgg ggccacagtg
     1621 ggtgcaggtc aacgagtgcg cgggcaccac cgtcggcgtc cgcgccagcc agcccgggga
     1681 cgccctgggc cggcccatgt ccacgcgctt ctcctaccag tggcagaacg cggccggtgc
     1741 ctggctgccc gtccccggcg ccggctcgcc ctggctggcc gccggccccg ggccgtggct
     1801 caaccgcgag accggctgga cccagacctt cctctcgggc gcgggcacgc gcgtgcgcgg
     1861 ggtggtccag atgcgatggg gctccgccgc cggccgtacg ctggtcacgc cgggctcctg
     1921 cctgctgcgc tgatgcacat ccgccgcgcg ggcctggccg acgccgacgc ggtgctggcc
     1981 gtctgggagg ccgcgcgcag cgccggcgcc gaccgcccgg acgacctggc cgagtacgcc
     2041 ctgctgctcg agcgcagccc cgacggtgtg ctggtggccg aggtggacgg ccgcgtggtc
     2101 gggaccgtga tcgccgtctg ggacggctgg cgcggcgcaa tcgcccggct gtgcgtgctg
     2161 ccctcgcacc ggcgccgggg catcggccac gcgctggtgg ccgccggcga ggaggagctg
     2221 cgggccaagg gcgtccggcg cgtgaacgtc ctgtctacga ggaggacgcc ggcgccggcg
     2281 acctgtgggc ctcgcgcgga tacgccacca acccgcgcat cgcgcgctac tggcgcgacc
     2341 tc
//
LOCUS       DASUPW010000451         2077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1212628, whole genome shotgun sequence.
ACCESSION   DASUPW010000451 DASUPW010000000
VERSION     DASUPW010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212628"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VFY44_10170"
     CDS             <1..769
                     /locus_tag="VFY44_10170"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5910850.1"
                     /translation="GDGVWGPAEDPENAKAVLRRAVELGVQLFDTADSYGPEVAEDLL
                     REALHPYPDDILIATKAGLTRTGPGEWHAVGRPEYLRQQCELSLRRLKLDRIDLWQLH
                     RIDSKVDRDEQFAVLRDLKDEGKVGAVGLSEVSVEDIEAARRVVDVVTVQNLHNLTYR
                     KSQDVLEYCEREGIGFIPWFPIASGQLAQEGGPVDDVVKATGAEPSQVALAWLLQVSS
                     VMLPIPGTKSIEHLESNQAAAELELSDDQMAELTAAA"
     gene            complement(783..1262)
                     /locus_tag="VFY44_10175"
     CDS             complement(783..1262)
                     /locus_tag="VFY44_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910851.1"
                     /translation="MKTFSAALAALLAVSAVAAATAQAVTPKTGKWKVTLDGVPVPPG
                     GTAGGFKVSEKKGKLYVSNFLSPEYYVKCTGQQAALVERNPGFPTTWGTSNAKKLSAG
                     AGFKGRFPFKSGKTTRADSYTGKFTSRKKASGTVRTTIKGDPLRACDSGALKWKATR"
     gene            complement(1317..>2077)
                     /locus_tag="VFY44_10180"
     CDS             complement(1317..>2077)
                     /locus_tag="VFY44_10180"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX5910852.1"
                     /translation="ARVSDALPRALQIPLGGTATGTGLNTHADFAEKARAKLSEATGL
                     AVAAPEDPFEAQGNRDGLVELSGAMKVVAVSLTKIANDLVIMGSGPRTGLVELSLPEL
                     QKGSSIMPGKVNPVIPEVVLQVAAQVIGNDAAVTIAGTQGNFELNVRVPVIARNVLDS
                     ITILSSACTMLAEKCVDGLEAREENLTRAAESTPAIATALNPFIGYDKATEIVKEAVA
                     SERTIREVAIEKGVDEATLDEALDLRKMARGSAG"
BASE COUNT          293 a          727 c          738 g          319 t
ORIGIN      
        1 cggggacggg gtgtgggggc cggccgagga ccccgagaac gcgaaggcgg tcctgcggcg
       61 cgcggtcgag ctgggggtcc agctgttcga caccgccgac tcgtacgggc ccgaggtggc
      121 cgaggacctg ctgcgcgagg cgctgcaccc gtaccccgac gacatcctga tcgccaccaa
      181 ggccggcctc acgcgcacgg gcccgggcga gtggcacgcg gtcggccgtc ccgagtacct
      241 gcgccagcag tgcgagctca gcctgcgccg gctgaagctc gaccggatcg acctgtggca
      301 gctgcaccgg atcgactcca aggtcgaccg cgacgagcag ttcgcggtgc tcagggacct
      361 caaggacgag ggcaaggtcg gcgccgtcgg cctgtccgag gtgtcggtcg aggacatcga
      421 ggccgcgcgc cgggtcgtgg acgtcgtgac cgtccagaac ctgcacaacc tgacctaccg
      481 caagtcacag gacgtgctcg agtactgcga gcgcgagggc atcggcttca tcccctggtt
      541 ccccatcgcc agcggccagc tggcccagga gggcggcccg gtggacgacg tggtcaaggc
      601 caccggcgcc gagccgtcgc aggtggcgct ggcgtggctg ctccaggtgt cgtcggtcat
      661 gctgcccatc ccgggcacca agagcatcga gcacctggag tccaaccagg ccgccgccga
      721 gctggagctc agcgacgacc agatggccga gttgacggcc gccgcctagc ccggccaccc
      781 ggctagcggg tggccttcca cttcagggcg ccggagtcgc atgcgcgcaa ggggtcgccc
      841 ttgatggtcg tgcgcaccgt gccgctggcc ttcttcctgg acgtgaactt gcccgtgtag
      901 ctgtcggcgc gggtggtctt gcccgacttg aacgggaatc gccccttgaa gccggcgccg
      961 gcgctgagct tcttggcgtt cgaggtcccc caggtggtcg ggaagccggg gttcctctcc
     1021 accagcgcgg cctgctgtcc cgtgcacttg acgtagtact cgggcgagag gaagttgctc
     1081 acgtagagct tgcccttctt ctcggagacc ttgaagccgc cggctgtgcc gccggggggc
     1141 acgggcacgc cgtccaacgt gaccttccac ttaccggtct tgggcgtgac ggcctgggcg
     1201 gtcgcggcgg cgacggcgga cacggcgagc agggccgcga gcgcggcgga gaaggtcttc
     1261 atgcgcgcca tccgaacacg gatgcccgga tggcgccact agcagggggg ccagacctaa
     1321 ccggcactcc cgcgtgccat cttgcgcagg tccagcgcct cgtccagcgt cgcctcgtcc
     1381 acgcccttct cgatcgcgac ctcgcggatc gtgcgctcgg aggccacggc ctccttgacg
     1441 atctccgtgg ccttgtcgta gccgatgaac gggttcagcg cggtggcgat ggccggcgtc
     1501 gactcggccg cgcgcgtcag gttctcctca cgcgcctcca gcccgtcgac gcacttctcg
     1561 gccagcatcg tgcaggcgct ggacaggatc gtgatcgagt ccagcacgtt gcgggcgatc
     1621 accggcacgc gcacgttcag ctcgaagttg ccctgggtgc cggcgatggt cacggctgcg
     1681 tcgttgccga tcacctgcgc ggccacctgc agcaccacct cggggatcac cgggttgacc
     1741 ttgccgggca tgatcgacga gcccttctgc agctcgggca gcgacagctc caccagcccg
     1801 gtgcgcgggc cggagcccat gatcaccagg tcgttggcga tcttggtcag cgagaccgcc
     1861 accaccttca tggcgccgga cagctccacg agcccgtcgc ggttgccctg ggcctcgaac
     1921 ggatcctcgg gggcggccac ggccaggccg gtggcctcgg acagcttggc ccgtgccttc
     1981 tcggcgaagt cagcgtgggt gttcaggccg gtgcccgtgg cggtgccgcc gagcgggatc
     2041 tgcagcgcgc gcggcagcgc gtcggacaca cgcgcgc
//
LOCUS       DASUPW010000452         2411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_103809, whole genome shotgun sequence.
ACCESSION   DASUPW010000452 DASUPW010000000
VERSION     DASUPW010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103809"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VFY44_10185"
     CDS             <1..487
                     /locus_tag="VFY44_10185"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEX5910853.1"
                     /translation="STIGNLTPGPRADFLAALADTLAPGDTLLLGTDLVKDPATLEAA
                     YDDSEGLTAAFNKNVLTVINRELGGEFDLDAFDHVSRWDEGYQRIEMRLRSRQAQSVP
                     VSALGLTAEFAAGEEMRTEVSCKFTPERVADELEAAGLRMEAFHTDADGLFALSRASL
                     R"
     gene            503..1270
                     /locus_tag="VFY44_10190"
     CDS             503..1270
                     /locus_tag="VFY44_10190"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5910854.1"
                     /translation="MNLDGAGALISGGASGLGAATARLLVEKGARVALIDLNADAAHA
                     LAGELGDAAHAVPCDVTDPGEMEAAVDEAATLLGGLRLAVGCAGIGWAERTVHKRGAH
                     SFEPYEKVIQVNLIGMFNLLRLGANAIAQSEPGDDGERGAVVMTASIAAFDGQIGQLS
                     YSASKGGVVGMTLPAARDLAGRGVRVNTIAPGLFDTPLLAALPDDARKALGDGVPFPQ
                     RLGKPSEYAALVASIAENTMLNGETIRLDGALRMPPK"
     gene            1282..>2411
                     /locus_tag="VFY44_10195"
     CDS             1282..>2411
                     /locus_tag="VFY44_10195"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX5910855.1"
                     /translation="MPEAVIVDAVRTPIGRAFKGSLAQVRPDETGAFIVDQLLERNPG
                     VDPALVEEVIAGCGMPQGKQAFNIGRLIALLSQKLPLETNGVTVSRYCASGLEAIRIA
                     SNNIKAGQGDAYIAAGVEFVSQFNERTEAAGAADQNEHLKGAAEGEPDFYIAMGDTAV
                     NVAKRYDVKRSDMDEYAQRSQELAVQSQESGFFDKEIVPVTLPDGTVVDKDDGPRASS
                     TLEKLSELPEAFEGGGGVTAGNSCPLNDGAAASLIMSDTKAKELGLTPRARIITAATA
                     ANEPEYMGVAPIWAIGKVLERAGMSIDDVDTVELNEAFAAQVIPIMAECDIPLEKMNP
                     HGGAIALGHPFGMTGVRIMTTLLNGLEADDKTIGLETMCVAGG"
BASE COUNT          382 a          877 c          844 g          308 t
ORIGIN      
        1 ctccaccatc ggcaacctga cgcccggccc gcgcgcggac ttcctcgccg cgctcgccga
       61 caccctggcg cccggcgaca cgctgctgct gggcaccgac ctggtcaagg acccggccac
      121 gctggaggcg gcctacgacg actccgaggg cctgaccgcg gcgttcaaca agaacgtgct
      181 gacggtgatc aaccgcgagc tgggcggcga gttcgacctg gacgcgttcg accacgtctc
      241 gcgctgggac gagggctacc agcggatcga gatgcggctg cggtcgcgcc aggcccagtc
      301 ggtgccggtg tccgcgctgg ggctgaccgc agagttcgcc gccggcgagg agatgcggac
      361 cgaggtctcg tgcaagttca ccccggagcg ggtggccgac gagctcgagg ccgccggcct
      421 gcgcatggag gccttccaca cggacgccga cgggctgttc gcgctcagcc gtgcctccct
      481 ccgctgagta gggtctcccg ccatgaacct ggacggagcg ggagcactga tcagcggcgg
      541 ggcgtccggg ctgggcgccg ccaccgcacg actgctggtc gagaagggcg cgcgcgtcgc
      601 gctcatcgac ctgaacgccg acgccgccca cgccctggcg ggcgagctgg gcgacgcggc
      661 ccacgccgtg ccctgcgacg tgaccgaccc gggcgagatg gaggccgccg tggacgaggc
      721 ggccaccctg ctgggcggcc tgcgcctggc cgtcggctgc gccgggatcg gctgggccga
      781 gcgcacggtg cacaagcgcg gcgcccactc cttcgagccc tacgagaagg tcatccaggt
      841 caacctgatc gggatgttca acctgctgcg cctgggcgcg aacgccatcg cccagtccga
      901 gcccggcgac gacggcgagc gcggcgcggt ggtcatgacc gcgtcgatcg cggccttcga
      961 cggccagatc ggccagctgt cctactcggc ctccaagggc ggggtggtcg gcatgaccct
     1021 gcccgccgcg cgcgacctgg ccgggcgcgg cgtgcgcgtg aacaccatcg cccccggcct
     1081 gttcgacacc ccgctgctgg ccgcgctgcc cgacgatgcg cgcaaggccc tcggcgacgg
     1141 cgttcccttc ccgcagcgac tcggcaagcc ctcggagtac gccgcgctgg tggcgtcgat
     1201 cgccgagaac accatgctca acggagaaac gatccggctg gacggcgctc tgcgcatgcc
     1261 gccgaagtaa ggagactcgc aatgcccgaa gcagtcatcg tggacgccgt ccgcaccccc
     1321 atcggccgcg ccttcaaggg ctccctggcc caggtccgcc ccgacgagac cggcgccttc
     1381 atcgtcgacc agctcctgga gcgcaacccc ggcgtggacc ccgcgctggt cgaggaggtc
     1441 atcgccggtt gcggcatgcc ccagggcaag caggcgttca acatcggccg gctgatcgcc
     1501 ctgctgtcgc agaagctgcc gctggagacc aacggcgtga ccgtctcgcg ctactgcgcc
     1561 tccggcctcg aggccatcag gatcgcgtcg aacaacatca aggccggcca gggcgacgcc
     1621 tacatcgccg ccggcgttga gttcgtctcc cagttcaacg agcgcaccga ggccgccggc
     1681 gccgccgacc agaacgagca cctcaagggc gcggccgagg gcgagcccga cttttacatc
     1741 gccatgggcg acacggcggt gaacgtggcc aagcgctacg acgtcaagcg gtccgacatg
     1801 gacgagtacg cccagcgctc gcaggagctg gccgtgcagt cgcaggagag cggcttcttc
     1861 gacaaggaga tcgtgcccgt cacgctgccc gacggcacgg tggtcgacaa ggacgacggc
     1921 ccgcgcgcct cgtccacgct ggagaagctg tccgagctgc ccgaggcgtt cgagggcggc
     1981 ggcggcgtga ccgccggcaa ctcgtgtccg ctgaacgacg gcgccgcggc gtcgctgatc
     2041 atgtccgaca ccaaggccaa ggagctgggc ctgacgccgc gagccaggat catcaccgcg
     2101 gccacggccg ccaacgagcc ggagtacatg ggcgtggccc ccatctgggc catcggcaag
     2161 gtgctggagc gcgccggcat gagcatcgac gacgtggaca cggtcgagct gaacgaggcg
     2221 ttcgccgccc aggtcatccc gatcatggcc gagtgcgaca tcccgctgga gaagatgaac
     2281 ccgcacggcg gcgcgatcgc cctgggccac ccgttcggca tgaccggcgt gcgcatcatg
     2341 accacgctgc tgaacggcct cgaggccgac gacaagacca tcggcctgga gaccatgtgc
     2401 gtggccggcg g
//
LOCUS       DASUPW010000453         5439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_658201, whole genome shotgun sequence.
ACCESSION   DASUPW010000453 DASUPW010000000
VERSION     DASUPW010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658201"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VFY44_10200"
     CDS             complement(<1..469)
                     /locus_tag="VFY44_10200"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5910856.1"
                     /translation="MTRRRPWAVAAAAVACCAAAAAIAVPSSGDSDYRVDVVFDDSRG
                     LIPGQLVQVAGARVGSIEDVSVTPGYKARIHMRVDRRFAPFRADARCTIKPQGLIAEN
                     YVQCDPGSPDARELRGRGGRAPTVPVERTTQPVSLTDLFEVWNTPTRHRLTVLL"
     gene            complement(466..1794)
                     /locus_tag="VFY44_10205"
     CDS             complement(466..1794)
                     /locus_tag="VFY44_10205"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5910857.1"
                     /translation="MSRARKLRLVAVGALCAVAAVVGALALGVGSSGYVVNAQFSDGS
                     QLVTGDLVQVGGRKVGKVSAIGLTDDGLAEVEMTLDGDVAPLRRGTRASVRTVGLSGV
                     ANRFVDLSPGPESAGEIPDGGVLGPDDTRGVVDLDALLNGFDPKARRDIRGIVRDASV
                     ALNPETARQTNAGLAVLNPAVGQLTELNRELTRDDAALRSMLTHTASVSGVLARHRDG
                     LATGIDATAGVFDAVAAEREALADSLARAPGALAMSRRTLRRVRTRTLPVLDPVVRDT
                     RPALKPLGQLLEVVEPTVGDALPLLTEVRRLVPQARRTLAPLPRLERPASPAIASTTK
                     ALRQARPLLAGTRPYTPELVAGFVTGLGGTAAAAYDANGHYNRINLLATANTLTGLLP
                     ALPAGRLGGLRTRLDARCPGAAEEPAPDRSNPWAAGPGSGAGTCDPGHSP"
     gene            complement(1791..2594)
                     /locus_tag="VFY44_10210"
     CDS             complement(1791..2594)
                     /locus_tag="VFY44_10210"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5910858.1"
                     /translation="MRAWIHRSRAGGALYGLGTAGSFTADVVGGVFSRRVWPYLGEVL
                     RQAGVFITGSMLIILALVFVLGLQCGIEGAYGAKAVGASTSVGAFTALCNLREVIPYA
                     FGYMMAAKVGTGIVAELGSMRISEEIDAVSVMGLDAVAYLGSTRLLGCMIVLPFIYMI
                     AIVCGITASYLAVVIQLGQVSAGGYLQLFWMFQTPLDFVFSLVKGMAMAAFVVLVGIY
                     YGYHASNGPVGVGRATAKSMVANIIGIHTIGLLGTQLFWGSNPRAPIGG"
     gene            complement(2591..3373)
                     /locus_tag="VFY44_10215"
     CDS             complement(2591..3373)
                     /locus_tag="VFY44_10215"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5910859.1"
                     /translation="MRASTGSALSELGQIGALTVRTVNAAVRPPYTYGPELASQFVFG
                     LRMAAIPLVISSIAFTYGPAGVQAAGFLNLFGAIDRLGGLFAVIVMREFAPLVCAIVM
                     AGVVGTSICADLGARQIREELDALRVLGVDTTKSLVAPRFIALMALASLSNVFALLLG
                     TLGGMIVTLANGAPLGPFFATFFTNATVPEIAASLIKTTLFGAIIAIVCCYKGLTVKG
                     GPEGVGRAVNQAVVISFMAIGIVNYAFTQALLALYPELSVVR"
     gene            3603..>5439
                     /locus_tag="VFY44_10220"
     CDS             3603..>5439
                     /locus_tag="VFY44_10220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX5910860.1"
                     /translation="MGGRASRRVLTCVAGAAMALAAAGPAAAAETRQELALTTSDGVA
                     LHATVAGEAPLRPRPLVVEYSPYGQDASAHPPLGPDFNYVYVQARGTGRSGGAWDIMG
                     DREQLDIAETLGQLCRRPFSSGRIGLYGFSASAIASYHAMRRGSLPCVKAASMLAGTN
                     SLYRDLIFIGGMPNLVPAGVVTGAIAGAFLANLPGRAGNPASGLSAPGGVTNALNAFL
                     AHQTEDDFWRRRIFPGPGGAPRDIPILAATGFYDVESRGPFENFKAIRSRGGRLLVIG
                     AHDREAGGSGGVQPHFRRWFEHHLLGRDNGADREPPVQLYVGRGSHGDLIGGRWVKVD
                     ARDWPVPGTRWRALHPATDGSLSPARPSRGATQSVPGVPSNTLSTDPYTTATVTNVQG
                     SNLSEATALTYTTPRLAADVMTVGPAALRVRFASTSPEADIYAVLSDVASDGTSNPVA
                     AGRLRNSFPRLEPGRSLTDPDSGAVVQPFNDFSRKEPAAAGQARDYQVEFWPIGNRFE
                     RGHRLRLSLVGAPVSFLPGAPGVNTITVGGPEAAALQVPTLPGSDLCAALGARPCPTA
                     APAATQPCLSARAVVGRRGIGRVRLGMTRARLRAIGAGRPRRTART"
BASE COUNT          753 a         2051 c         1978 g          657 t
ORIGIN      
        1 acagcagcac ggtcagccgg tggcgggtcg gcgtgttcca gacctcgaac aggtcggtca
       61 ggctcacggg ctgggtggtg cgctccaccg ggacggtcgg ggcccggccg ccccgcccgc
      121 gcagctcgcg cgcgtcgggc gagccggggt cgcactggac gtagttctca gcgatcaggc
      181 cctggggttt gatcgtgcag cgggcgtccg cacggaaggg agcgaaccgg cggtccacgc
      241 gcatgtggat ccgggccttg tagcccgggg tcaccgacac gtcctcgatc gagcccacgc
      301 gcgcgccggc gacctgcacc agctgcccgg ggatcagccc gcgcgagtcg tcgaagacca
      361 cgtcgaccct gtagtccgag tcgccggagg agggcaccgc gatggccgcg gcggccgcgc
      421 agcacgccac cgcggctgcc gcgacggccc agggacgccg gcgggtcacg ggctgtgccc
      481 cgggtcgcag gtgcccgccc cggacccggg cccggcggcc caggggttcg agcggtcggg
      541 ggccggctcc tcggccgccc cggggcagcg cgcgtccagc cgcgtccgca gcccgcccag
      601 cctgccggcg ggcagcgcgg ggagcagccc ggtcagcgtg ttggcggtgg ccagcaggtt
      661 gatccggttg tagtggccgt tcgcgtcgta ggcggccgcc gccgtgcctc cgagcccggt
      721 cacgaacccg gccaccagct ccggggtgta cgggcgggtg ccggccagca gcggccgggc
      781 ctgccgcagg gccttggtcg tcgacgcgat cgcgggcgac gcgggccgct ccagccgggg
      841 cagcggcgcg agcgtgcgcc gggcctgggg caccagccgg cggacctcgg tcagcagcgg
      901 cagggcgtcg cccaccgtcg gctcgacgac ctcgagcagc tggccgagcg gcttcagggc
      961 ggggcgcgtg tcgcgcacca cggggtccag gacgggcagc gtgcgcgtac gcacgcgccg
     1021 cagggtccgc cgggacatgg ccagcgcgcc gggcgcgcgc gccagcgagt cggccagcgc
     1081 ctcacgctcc gcggcgaccg cgtcgaacac gcccgcggtg gcgtcgatgc cggtggccag
     1141 cccgtcgcgg tggcgcgcca gcacgccgga gaccgaggcg gtgtgggtga gcatggaccg
     1201 cagggcggcg tcgtcgcggg tcagctcgcg gttgagctcg gtcagctgtc ccaccgcggg
     1261 gttcagcacc gccaggccgg cgttcgtctg gcgcgcggtc tccgggttca gggccacgga
     1321 ggcgtcgcgc acgatgccgc ggatgtccct gcgcgccttg gggtcgaagc cgttgagcag
     1381 cgcgtccagg tccaccaccc cgcgggtgtc gtcgggcccg aggacgccgc cgtcgggtat
     1441 ttcgccggcg gactcgggcc ccggggacag gtcgacgaag cggttggcca cgcccgacag
     1501 gccgacggtc cggaccgacg cccgggtgcc gcggcgcagc ggcgcgacgt cgccgtccag
     1561 ggtcatctcc acctcggcca gcccgtcgtc ggtcagcccg atcgcggaca ccttgcccac
     1621 cttgcggccc cccacctgga ccaggtcgcc ggtgaccagc tggctgccgt cggagaactg
     1681 ggcgttgacc acgtagcccg agctgccgac ccccagggcc agcgccccca ccacggcggc
     1741 gacggcgcac agcgcgccca cggccaccag cctgagcttg cgggcgcggc tcatccgccg
     1801 atcggtgccc gtgggttgga gccccagaag agctgggtgc ccagcagccc gatggtgtgg
     1861 atgccgatga tgttggccac catcgacttg gcggtggcgc ggcccacgcc caccgggccg
     1921 ttgctggcgt ggtagccgta gtagatgccc acgagcacca cgaacgccgc catggccatg
     1981 cccttgacca gcgagaacac gaagtccagc ggcgtctgga acatccagaa cagctgcagg
     2041 tagccgcccg ccgagacctg gcccagctgg atcaccacgg ccaggtacga cgccgtgatg
     2101 ccgcacacga tcgcgatcat gtagatgaac ggcaggacga tcatgcagcc cagcaggcgg
     2161 gtcgagccca ggtaggccac ggcgtccagg cccatcacgc tgaccgcgtc gatctcctcg
     2221 gagatgcgca tggagcccag ctcggccacg atgcccgtgc ccaccttcgc cgccatcatg
     2281 tagccgaagg cgtaggggat cacctcgcgc aggttgcaga gcgcggtgaa ggcgcccacc
     2341 gacgtgctgg cccccacggc cttggcgccg taggcgcctt cgatgccgca ctgcaggccc
     2401 agcacgaaca ccagcgccag gatgatcagc atcgagccgg tgatgaacac cccggcctgt
     2461 cgcagcacct cgcccaggta gggccagacc cggcggctga acacgccgcc gaccacgtcc
     2521 gcggtgaacg agccggcggt gcccaggccg tacagcgcgc cgccggcacg gctccggtgg
     2581 atccaggccc tcaccggacc accgacagct cggggtagag cgccagcagc gcctgggtga
     2641 acgcgtagtt cacgatgccg atcgccatga acgagatcac cacggcctgg ttcacggccc
     2701 gcccgacgcc ctcgggcccg cccttgacgg tcagcccctt gtaacagcag acgatcgcga
     2761 tgatggcgcc gaacagcgtg gtcttgatca gcgacgcggc gatctcgggc acggtcgcgt
     2821 tggtgaagaa ggtggcgaag aagggcccga gcggagcgcc gttggccagc gtcacgatca
     2881 tgccgccgag cgtgcccagc agcagggcga acacgttgga cagcgacgcc agcgccatca
     2941 acgcgatgaa ccgcggcgcc accaggctct tggtcgtgtc cacaccgagg acccgcaggg
     3001 cgtccagctc ctcccggatc tggcgggcac cgaggtcggc gcagatcgag gtccccacca
     3061 ccccggccat gacgatcgcg cagaccagcg gcgcgaactc gcgcatcacg atcaccgcga
     3121 acagcccgcc cagccggtcg atggcgccga agaggttcag gaacccagcg gcctgcacac
     3181 ccgccggccc gtaggtgaag gcgatggacg agatcaccag cgggatcgcc gccatcctca
     3241 ggccgaacac gaactggctg gccagctccg ggccgtaggt gtagggcggg cgcaccgccg
     3301 cgttgaccgt gcgcaccgtc agcgctccga tctgacccag ctcggagagg gcggagcccg
     3361 tgctcgcgcg cacgcgcccg aggggccgcc gccggcccgt cgacaactcc cctgcagtgc
     3421 cgatgttcgc cacttctgcc ctcgctcccg atgcccgccc gtcagcgcga tcgcgccaag
     3481 cggtcgccga ccgtatcagc gcggtcgtgc cggcgcgcca ccagcgaaca gcggtgttcg
     3541 taacccggca cgacggcaac gtaccgcacc ggcgaacatc aatgttcgct atcgtccagc
     3601 gcatgggagg ccgggccagc aggcgggtgc tgacgtgtgt cgccggcgcg gcgatggcgc
     3661 tggccgccgc cgggcccgcg gccgcggcgg agacccggca ggagctggcc ctgaccacct
     3721 ccgacggtgt ggccctgcac gccaccgtgg ccggggaggc gccgctgcgc ccgcgcccac
     3781 tggtggtgga gtacagcccg tacggccagg acgcctcggc ccacccgccg ctgggccccg
     3841 acttcaacta cgtctacgtg caggcccgcg gcaccgggcg cagtggcggg gcgtgggaca
     3901 tcatgggcga ccgcgagcag ctggacatcg cggagacgct gggccagctc tgccggcggc
     3961 cgttcagctc cgggcggatc ggcctgtacg ggttctcggc cagcgcgatc gcgtcctacc
     4021 acgccatgcg ccgcggctcg ctgccgtgcg tgaaggccgc gtccatgctc gcgggcacca
     4081 actcgctcta ccgggacctg atcttcatcg gcggcatgcc caacctggtt ccggcgggag
     4141 ttgtcaccgg cgccatcgcg ggcgccttcc tggccaacct gccggggcgg gcgggcaacc
     4201 ccgcgtccgg gctctccgcg cccggcgggg tgaccaacgc gctgaacgcg ttcctggccc
     4261 atcagaccga ggacgacttc tggcgccggc ggatcttccc ggggcccggc ggggcgccgc
     4321 gggacatccc catcctggcc gccacgggct tctacgacgt cgagtcgcgg gggccgttcg
     4381 agaacttcaa ggccatccgc agccgcggcg gccggttgct ggtgatcggc gcccacgacc
     4441 gcgaggcggg cggcagcggc ggggtgcagc cccacttccg gcgctggttc gagcaccacc
     4501 tgctgggccg ggacaacggc gccgaccgtg agccgcccgt ccagctgtac gtcggtcgcg
     4561 gcagccacgg ggacctcatc ggcggcagat gggtcaaggt cgatgcgcgc gactggccgg
     4621 tgcccggcac gcgctggcgc gccctgcacc cggccaccga cggatcactg tcgccggccc
     4681 gtcccagccg gggcgcgacg cagtcggtcc ccggcgtgcc ctcgaacacg ctctccaccg
     4741 acccctacac caccgccacc gtgaccaacg tgcaggggtc caacctcagc gaggccaccg
     4801 cgctcaccta caccaccccc aggctcgcgg ccgacgtgat gaccgtcggc ccggcggccc
     4861 tgcgggtgcg cttcgccagc acctcgcccg aggccgacat ctacgcggtg ctgtccgacg
     4921 tggcgtccga cgggacctcc aaccccgtgg cggccggccg cctgcgcaac tcgttcccgc
     4981 ggctcgagcc cggcaggtcg ctgaccgacc ccgacagcgg ggcggtcgtc cagcccttca
     5041 acgacttctc gcgcaaggag cccgcggcgg ccgggcaggc gcgcgactac caggtggagt
     5101 tctggccgat cggcaaccgc ttcgagcgcg ggcaccggct gcggctgtcg ctcgtgggcg
     5161 cgcccgtgtc cttcctcccg ggcgcgcccg gggtgaacac catcaccgtg ggcggcccgg
     5221 aggcggcggc gctgcaggtg cccaccctgc ccggctcgga cctgtgcgcc gcgctggggg
     5281 ccaggccgtg cccgacggcc gcgcccgccg ccacgcagcc gtgcctgtcc gcgcgggcgg
     5341 tggtcgggcg cagggggatc ggccgtgtgc ggctgggcat gacccgcgcg cggctgcgcg
     5401 cgatcggcgc gggccggccg cgccgcacgg cgcgcaccc
//
LOCUS       DASUPW010000454         4618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1074740, whole genome shotgun sequence.
ACCESSION   DASUPW010000454 DASUPW010000000
VERSION     DASUPW010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074740"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VFY44_10225"
     CDS             <1..656
                     /locus_tag="VFY44_10225"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEX5910861.1"
                     /translation="IVFGVLLGLGVLPGDNPVGGTFVPAGIAGAVVVVLGLVALIPGD
                     FERRLSKASSDTRMGRLAAKLATGPATLSSGIRTAIDYIAHPSRGALGVFGAVGFWAS
                     QIGILWACFEAFGGSVPFAVLVQGFFVGMAANLIPSPAGGVGSVDAGMIGAFVLFGVD
                     SQIVFPAVLTYRIIAFWLPIPPGIVAFFQLRRTVARWDAERRSGGYTSESKVMTEAM"
     gene            653..1684
                     /gene="trxB"
                     /locus_tag="VFY44_10230"
     CDS             653..1684
                     /gene="trxB"
                     /locus_tag="VFY44_10230"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX5910862.1"
                     /translation="MSDGNGSDRKIEDVIIIGSGPAGYTAALYTSRADLQPLVFEGFA
                     WGGLLQQTTDVENYPGYPEGVMGPEMMQNFRDQAERFGTRFVTDDVTRVDLSEDGGVH
                     RVYVGDDEYLARSVILAMGAEPKKLGVTGEDELASRGVSYCATCDAAFFRDKPTIVVG
                     GGDTAMEDATFLAKFAKDVKIIHRRGEFRASKVMLNRAEATDNIELLTPYVIDRFEGG
                     GDNGALKKVFLENTEGGEDRELEIDGAFIAIGHKPNSDIVIGQVDTDDEGYVEVEGRS
                     TRTKLAGVFAAGDLVDHTYRQAVTAAGSGCAAALDAEAYLRDTPIDAEAHWEGDPDKM
                     VAAAEKAAS"
     gene            1681..2976
                     /locus_tag="VFY44_10235"
     CDS             1681..2976
                     /locus_tag="VFY44_10235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HEX5910863.1"
                     /translation="MTEVWTNWARQQRCAPVRIERPASEEELSRAVTRAAGEGLEVRV
                     AGSGHSFTDTACTDGVLIDIGAMDRVLDADASSGLVTVQAGITLHELGARLAELGLAM
                     ENQGDIDAQTLAGAISTATHGTGATYRNISSQVEAVRLVTATGEVLDIGAEDPGLLGA
                     ARVGLGALGALSAVTLRTVPIFTLERRDAPMPLGAVLDDFDGHADGADHFELYVFPYT
                     DVALTRTSTRSDREPEPGNVRVEVVQERLVENGALAVLGAVTGRLPGSIPRVNRTLIK
                     AMTPSVKVDRSHRVYATRRDVRFTEMEYAIPREHGPEALRRVLETIQRRRLPIAFPIE
                     MRVVRGDDAPLSTAHGRDSVYIAVHQFRGQEFETYFRAVEAIMDSYGGRPHWGKRHYQ
                     SAATLRDRYPQWDAWQEARDELDPGRTFTNDYTRRVLGG"
     gene            complement(2973..4160)
                     /locus_tag="VFY44_10240"
     CDS             complement(2973..4160)
                     /locus_tag="VFY44_10240"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase/aldolase"
                     /protein_id="HEX5910864.1"
                     /translation="MSPYDRLETAFAGRDAPFAFVDLDALWPNAHEMLGRAGPKPIRV
                     ASKSVRCRELLERILAHDPRFRGLMTFTLDESLWLHGLGFDDLLLAYPTADMGALAGL
                     GALEDDGRPIVMVDSVEHLDLIERAVPSPAAPVRVCLDMDAGFHMAGGRVRIGPKRSR
                     VRTPPQAVALAREVVRRDGFELAGLMAYEGHIAGVGDRPRGNPLQGLVIGRMQAASAS
                     ELAERRAAVVEAVTAIAPLALVNGGGTGSLHTTAREQAVTELTAGSGFYAPTLFDRYS
                     AFSLRPAAGFALPVVRRPGPETVTLLGGGYHASGAAGKDRLPEPWLPEGLKLDSLEGA
                     GEVQTPVTGDAARTLDLGDRVYMRHAKAGELCERFDSLLLVSGDAVVGEVPTYRGEGK
                     TFL"
     gene            complement(4157..>4618)
                     /locus_tag="VFY44_10245"
     CDS             complement(4157..>4618)
                     /locus_tag="VFY44_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910865.1"
                     /translation="DLAARPEERSGRELVIENGRGLKTQSQYRGLRNDRYVYIRHDKT
                     GERELYDIKRDPRQLENLAKDERFRRVRILMDRRVRKLSRCRGVRACGASRPKISIRA
                     DCREGPRYGRPVVGGREKSRVRRVTRAGGAGLQVTMVDGRVATFPDVACAQ"
BASE COUNT          643 a         1683 c         1676 g          616 t
ORIGIN      
        1 tgatcgtctt cggtgtgctg ctgggcctgg gcgtgctgcc gggcgacaac ccggtgggcg
       61 ggacgttcgt gccggcgggc atcgcgggcg cggtcgtggt ggtcctcggg ctggtcgccc
      121 tgatccccgg cgacttcgag cggcgactgt ccaaggcgtc cagcgatacc cgcatggggc
      181 ggctggcggc caagctggcc accgggccgg ccacgctgtc cagcggcatc cgcaccgcga
      241 tcgactacat cgcccacccc agtcgcggcg cgctgggcgt gttcggcgcc gtcgggttct
      301 gggcctcgca gatcgggatc ctgtgggcct gcttcgaggc cttcgggggc agcgtcccgt
      361 tcgccgtgct ggtccagggc ttcttcgtgg gcatggcggc caacctgatt ccctcgccgg
      421 cgggcggcgt aggctccgtg gacgccggca tgatcggcgc gttcgtcctc ttcggggtgg
      481 acagccagat cgtgttcccg gcggtgctca cgtaccggat catcgccttc tggctgccca
      541 tcccgcccgg gatcgtcgcg ttcttccagc tgcgccgcac cgtggcccgc tgggatgccg
      601 aacgccgctc ggggggctat acttcagaaa gtaaagtaat gacggaggcc atgtgagcga
      661 cggaaacggc agcgaccgca agatcgaaga cgtgatcatc atcggcagtg ggcccgcggg
      721 ctacacggct gcgctgtaca cctcgcgcgc cgacctgcag ccgctggtct tcgagggctt
      781 cgcgtgggga ggcctgctcc agcagaccac cgacgtggag aactacccgg gctaccccga
      841 gggggtcatg ggcccggaga tgatgcagaa cttccgcgac caggccgagc gcttcggcac
      901 ccgcttcgtg accgacgacg tcacccgcgt ggacctgtcc gaggacggcg gcgtgcaccg
      961 ggtctacgtc ggcgacgacg agtacctggc ccgcagcgtg atcctggcca tgggcgccga
     1021 gcccaagaag ctcggcgtga ccggcgagga cgagctggcc tcccgcggtg tgtcgtactg
     1081 cgccacctgc gacgccgcct tcttccgcga caagcccacg atcgtggtcg gcggcggcga
     1141 caccgccatg gaggacgcca ccttcctggc caagttcgcc aaggacgtga agatcatcca
     1201 ccgccgcggc gagttccgcg cgtcgaaggt gatgctcaac cgggccgagg ccaccgacaa
     1261 catcgagctg ctcacgccct acgtcatcga ccgcttcgag ggcggcggcg acaacggcgc
     1321 cctgaagaag gtgttcctgg agaacaccga gggcggcgag gaccgcgagc tcgagatcga
     1381 cggcgcgttc atcgccatcg gccacaagcc caactcggac atcgtgatcg ggcaggtgga
     1441 caccgacgac gagggctacg tggaggtcga gggccgctcg acccggacga agctggcggg
     1501 cgtgttcgcc gccggcgacc tggtggacca cacctaccgc caggccgtga ccgcggccgg
     1561 ctcgggctgt gccgccgcgc tggacgcgga ggcctacctc cgcgacaccc cgatcgacgc
     1621 cgaggcccac tgggagggcg acccggacaa gatggtcgcc gccgccgaga aggcagccag
     1681 ctgaccgagg tctggacgaa ctgggcgcgc cagcagcgct gcgccccggt ccggatcgag
     1741 cggccggcct ccgaggagga gctgagccgc gcggtgaccc gggccgccgg cgaagggctc
     1801 gaggtccgcg tggccggctc gggccactcg ttcaccgaca ccgcctgcac cgacggggtg
     1861 ctgatcgaca tcggcgccat ggaccgggtg ctggacgccg acgcctccag cggcctggtc
     1921 accgtccagg ccggcatcac cctgcacgag ctgggcgcgc gcctggccga gctgggcctg
     1981 gcgatggaga accagggtga catcgacgcg cagacgctgg ccggggccat ctcgaccgcc
     2041 acccacggca ccggggcgac ctaccggaac atctcgtccc aggtggaggc cgtccggctg
     2101 gtcacggcca ccggtgaggt gctggacatc ggtgccgagg accccggcct gctgggcgcg
     2161 gcgcgcgtgg gcctgggcgc gctgggcgct ctcagcgcgg tcacgctccg gacggtcccg
     2221 atcttcaccc tcgagcgccg cgacgcgccg atgccgctgg gcgcggtgct ggacgacttc
     2281 gacggccacg ccgacggcgc cgaccacttc gagctgtacg tgttcccgta caccgacgtg
     2341 gcgctcacgc gcacctccac gcgcagcgac cgcgagcccg agccgggcaa cgtgcgcgtc
     2401 gaggtggtgc aggagcggct ggtcgagaac ggcgcgctgg ccgtcctcgg cgccgtcacc
     2461 ggccggctcc ccgggtcgat cccgcgtgtg aaccggacgc tgatcaaggc catgaccccg
     2521 tcggtcaagg tcgaccgcag ccaccgggtg tacgccacgc ggcgcgacgt gcgcttcacc
     2581 gagatggagt acgcgatccc gcgcgagcac ggtcccgagg ccctgcgccg ggtgctggag
     2641 accatccagc gccgccggct gcccatcgcc ttcccgatcg agatgcgcgt ggtcaggggc
     2701 gacgacgcgc cgctgtccac cgcgcacggc cgcgacagcg tgtacatcgc cgtgcaccag
     2761 ttccgcgggc aggagttcga gacctacttc cgggccgtcg aggcgatcat ggactcctac
     2821 ggcgggcgcc cgcactgggg caagcgccac taccagtcgg cggccacgct gagggaccgt
     2881 tacccgcagt gggacgcctg gcaggaggcg cgcgacgagc tggaccccgg ccgcacgttc
     2941 accaacgact acacgcgccg cgtgctcggc ggctagagga aggtcttgcc ctcgccgcgg
     3001 taggtgggca cctcgccgac cacggcgtcg ccgctgacca gcagcaggga gtcgaagcgc
     3061 tcgcacagct cgcctgcctt ggcgtggcgc atgtacacgc ggtcgcccag gtccagcgtc
     3121 ctcgcggcgt cgccggtgac cggggtctgg acctcgccgg cgccctccag cgagtccagc
     3181 ttcaggccct cgggcagcca gggctcgggc agccggtcct tgcccgcggc gccggaggcg
     3241 tggtagccgc cgcccagcag cgtcaccgtc tccgggcccg gccggcgcac caccgggagg
     3301 gcgaagcccg cggcggggcg cagcgagaac gccgagtagc ggtcgaacag cgtgggggcg
     3361 tagaagcccg agcccgcggt cagctcggtc accgcctgct cgcgggccgt ggtgtgcagg
     3421 ctgcccgtgc cgccgccgtt gaccagcgcc agcggcgcga tggccgtgac cgcctcgacc
     3481 acggcggcgc ggcgctcggc gagttcggat gccgacgccg cctgcatccg gccgatgacc
     3541 aggccctgga gggggttgcc gcggggccgg tcgccgacgc cggcgatgtg gccctcgtac
     3601 gccatcaggc cggcgagctc gaagccgtcg cggcggacca cctcgcgggc cagcgcgacg
     3661 gcctggggcg gggtgcgcac ccgcgagcgc ttgggcccga tcctcacccg gccgcccgcc
     3721 atgtggaacc ccgcgtccat gtccaggcac acgcggacgg gcgcggcggg cgagggcacg
     3781 gcgcgctcga tcaggtccag gtgctcgacc gagtccacca tcacgatcgg ccgcccgtcg
     3841 tcctcgaggg cgcccagccc ggccagcgcg cccatgtcgg ccgtcgggta ggccagcagc
     3901 aggtcgtcga agcccagccc gtggagccac agcgactcgt ccagcgtgaa ggtcatcaac
     3961 ccgcggaaac gcgggtcgtg ggccaggatc cgctccagca gctcgcggca gcgcaccgac
     4021 ttgctggcca cccggatcgg cttgggcccg gcccggccca gcatctcgtg ggcgttgggc
     4081 cacagggcat ccaggtccac gaacgcgaac ggggcgtcgc ggccggcgaa cgcggtctcg
     4141 aggcggtcgt acgggctcac tgcgcgcacg ccacgtcggg gaaggtggcc acgcgaccgt
     4201 cgaccatcgt cacctgcagc ccggcaccgc ccgcacgcgt cacgcggcgc acgcgtgact
     4261 tctcgcggcc gcccacgacg gggcgcccgt agcgcggtcc ctcgcggcag tcggcgcgga
     4321 tcgagatctt cggccggctg gcgccgcagg cccgcacgcc gcggcatcgc gacagcttgc
     4381 gcacgcgccg gtccatcagg atccggacgc gccggaagcg ctcgtccttg gccaggttct
     4441 ccagttgccg cgggtcgcgc ttgatgtcgt acagctcgcg ctcgccggtc ttgtcgtggc
     4501 ggatgtacac gtagcggtcg ttgcgcagcc cgcggtactg cgactgggtc ttcagcccgc
     4561 ggccgttctc gatgaccagc tcgcggccgc tgcgctcctc gggccgcgcc gccaggtc
//
LOCUS       DASUPW010000455         2535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_521343, whole genome shotgun sequence.
ACCESSION   DASUPW010000455 DASUPW010000000
VERSION     DASUPW010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521343"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..554)
                     /gene="smpB"
                     /locus_tag="VFY44_10250"
     CDS             complement(75..554)
                     /gene="smpB"
                     /locus_tag="VFY44_10250"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX5910866.1"
                     /translation="MAGKKGKRKAAPGDVATNRQASFRYHLSDHVEAGIQLQGSEVKS
                     IRDGGVQIKDAYAFVRKGEVYLVNMHIAPYGPAARDGHEPERERKLLLHKYEIDRLTG
                     SVQEKGLTLVPTRVYFKNGRAKVEIALGRGKDQGDKRRDLKAKDQRREIDRALAERS"
     gene            586..2172
                     /locus_tag="VFY44_10255"
     CDS             586..2172
                     /locus_tag="VFY44_10255"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5910867.1"
                     /translation="MRRGALGLVVLMALVCAPSASAATNCPEPGANRWQKVTPAAAGM
                     DAAKLDAALAEGPQNLSLSVRVFRHGCLVAADPLEPTNRDTKFESWSMAKSVTSVMFG
                     RAVEQGLISPDDPVGSLLPEADREHGAITMRHLLTMTSGLHWNGLRDYNVFTGFDRTR
                     DALTLGTDHKPGTYFEYAQSPVSLLAEAIGRAAGQDAGTFVQRELFTPLGIEPGSWGW
                     TRDNAGHIGGFYGVQMRTEDYARMGELMRRGGVWKGKRLLSKTYMRDAITPTRTNGCY
                     GWLIWLNRDAPCVGPTITKRPVDDQREFPDLPADMYRFSGLFGQLVTVFPSQGIVVAR
                     NGQDAGLLPTGGSSWEHTLYRKVLGAVTDQRIAAPAPQANGGPQDKPNADYGFQTALL
                     NPDQYSKGVAQAPLPPAGPARARAMRLGPLNVRATPKGFLRVRVGCPPRWPAKVVTPL
                     CSGELRMEGTRKPVVYSVRPGRQKQVRFQLTRKRLSRLKKARKLPLNYSGRNPDATGG
                     TWTKASLTVLAAKPKKKRRR"
     gene            complement(2180..>2535)
                     /locus_tag="VFY44_10260"
     CDS             complement(2180..>2535)
                     /locus_tag="VFY44_10260"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5910868.1"
                     /translation="KDVAYVLRKHLRGLDHVYRVGGEEFVAVLPGVDLETAEQAAERL
                     RKAVEASRPAGLTVTISFGVVVASGVEAGDFDTLYRRADVALYEAKQKGRNGVRIAAD
                     DEAGPARRQTDAVPA"
BASE COUNT          352 a          954 c          866 g          363 t
ORIGIN      
        1 accgccaggc gcctccacac catgtggtga ttagacaccc tgggcccacc gggccgcaag
       61 ggggaacccc cggctcagct gcgctcggcc agcgcccggt cgatctcgcg gcgctggtcc
      121 ttggccttga ggtcgcgccg cttgtcgccc tggtccttgc cgcgccccag cgcgatctcg
      181 accttggcgc ggccgttctt gaagtagacc cgggtgggca ccagcgtcag gcccttctcc
      241 tgcaccgagc cggtcagccg gtcgatctcg tacttgtgca gcagcagctt gcgctcgcgc
      301 tcgggctcgt gcccgtcgcg ggcggccggg ccgtagggcg cgatgtgcat gttcaccagg
      361 tacacctcgc ccttgcgcac gaacgcgtag gcgtccttga tctggacgcc gccgtcgcgg
      421 atggacttga cctccgagcc ctggagctgg atgcccgcct cgacgtgatc gctcaggtgg
      481 tagcggaacg atgcctgcct gttggtggcc acatcacccg gcgccgcctt gcgctttccc
      541 ttcttaccgg ccatgccttc cattgtggcg atataggatg ggcacgtgag gcggggtgcc
      601 ctgggactgg tcgtgttgat ggcgctggtg tgcgcgccgt cggcctctgc ggccacgaac
      661 tgccccgagc ccggcgcgaa ccggtggcag aaggtcacgc ccgccgccgc cggcatggac
      721 gccgccaagc tcgacgccgc cctggccgag gggccgcaga acctcagcct gtcggtgcgc
      781 gtgttccgcc acggctgcct ggttgcggcc gacccgctcg agcccaccaa ccgcgacacg
      841 aagttcgaga gctggtcgat ggccaagtcg gtgacctcgg tcatgttcgg ccgggccgtg
      901 gagcagggcc tgatcagccc cgacgacccg gttggctcgc tgctgcccga ggccgaccgc
      961 gagcacggcg ccatcaccat gcggcacctg ctgaccatga cctcggggct gcactggaac
     1021 ggcctgcgcg actacaacgt gttcaccggg ttcgaccgca cgcgcgacgc gctgacgctg
     1081 ggcaccgacc acaagcccgg cacgtacttc gagtacgccc agagcccggt gtcgctgctg
     1141 gccgaggcca tcggccgcgc cgccggccag gacgccggca ccttcgtcca gcgtgagctg
     1201 ttcaccccgc tgggcatcga gcccggctcg tggggctgga cgcgcgacaa cgccggccac
     1261 atcggcggct tctacggcgt gcagatgcgc accgaggact acgcccgcat gggcgagctg
     1321 atgcgccgcg gcggcgtctg gaagggcaag cggctgctgt ccaagaccta catgcgcgac
     1381 gcgatcacgc ccaccaggac caacgggtgc tacggctggc tgatctggct gaaccgcgac
     1441 gcgccgtgcg tgggcccgac catcaccaag cgcccggtgg acgaccagcg cgagttcccc
     1501 gacctgccgg ccgacatgta ccgcttctcg ggcctgttcg gccagctggt cacggtgttc
     1561 ccgtcgcagg gcatcgtggt ggcccgcaac ggccaggacg ccgggttgct cccgaccggc
     1621 ggcagctcct gggagcacac gctctaccgc aaggtgctcg gggccgtgac cgaccagagg
     1681 atcgccgccc ccgcccccca ggccaacggg ggcccccagg acaagccgaa cgccgactac
     1741 ggcttccaga ccgcgctgct caaccccgac cagtactcca agggcgtcgc ccaggccccg
     1801 ctgccgcccg ccggccccgc ccgggcgcgc gccatgcggc tcggccccct gaacgtgcgc
     1861 gccacgccca agggcttcct gcgggtgcgc gtgggctgcc cgccgcgctg gccggcgaag
     1921 gtggtcacgc ctctgtgctc gggcgagctg cggatggagg gcacgcgcaa gccggtggtc
     1981 tactccgtcc ggccgggtcg ccagaagcag gtgcgcttcc agctcacccg caagcgcctc
     2041 tcacggctga agaaggccag gaagctgccg ctgaactact cgggccgcaa ccccgacgcc
     2101 accggcggca cctggaccaa ggcgtccctg acggtcctgg ccgccaagcc gaagaagaag
     2161 cgccggcgct agcgcccgct caggcgggaa cggcgtcggt ctgcctgcgc gcgggcccgg
     2221 cctcgtcgtc ggccgcgatc cggacgccgt tgcgcccctt ctgcttggcc tcgtacagcg
     2281 ccacgtcggc acgccggtag agggtgtcga agtcgccggc ctcgacgccc gaggccacga
     2341 ccacgccgaa gctgatggtc accgtcaggc cggccgggcg cgaggcctcg acggccttgc
     2401 gcaggcgctc ggcggcctgc tcggccgtct ccaggtccac gcccggcagg accgcgacga
     2461 actcctcgcc gcccacgcgg tagacgtggt cgagcccgcg caggtgcttg cgcagcacgt
     2521 aggccacgtc cttga
//
LOCUS       DASUPW010000456         2577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_384000, whole genome shotgun sequence.
ACCESSION   DASUPW010000456 DASUPW010000000
VERSION     DASUPW010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384000"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VFY44_10265"
     CDS             complement(<1..650)
                     /locus_tag="VFY44_10265"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5910869.1"
                     /translation="MAATDQVIKPEFGTAKKHFIFNEEHDALRESIGSFVEKELAPHA
                     ERWEEETFDDWVFERMGELGFLGLSYPEEYGGQGGDYYCNLVLAEEIVHADCGGLAMG
                     IAVHTDMATPPILAFGTEEQKQAYLAPAIRGEKISCLGITEPDAGSDVSGIKTRAVRD
                     GDEWVINGSKTYITNGHRADFIVLVTKTDPDAGYDGFTLFLVDMDLPGVIREKRLE"
     gene            complement(685..1569)
                     /locus_tag="VFY44_10270"
     CDS             complement(685..1569)
                     /locus_tag="VFY44_10270"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5910870.1"
                     /translation="MRRVPFIAVAALAAVPALPGTSQMGVYPGLRPLGDLAAPAPAQT
                     DSGLGVYPALTAPSALAFPGPAARRSASRYARSRGCKVSFAVADERGGVAGVGIDRPY
                     PAASLSKAMLLVAFLRRNADPTRSERETLGYMIRLSDNASADAIYRTLGAGPVRELAR
                     AAGMRRFAIAGHWAGATVTAADQARFFLALDDLTPPRHRAFERELLETVSPLQTWGIP
                     RVARPRWRVYFKGGWRPEHGTLLEHQAAYLERGNRRVAIAVLTEGGSDDRFGRATQAG
                     IARRLLAGSDGPLLTSEK"
     gene            1642..>2577
                     /locus_tag="VFY44_10275"
     CDS             1642..>2577
                     /locus_tag="VFY44_10275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5910871.1"
                     /translation="MSEREFDVVVVGAGPAGEVAAGRLGDAGLDVAVVEPELIGGECS
                     YWACMPSKALLRPGELYDETQRVPGVAEAVGGGLDTAAVLARRDEVVHDFDDSGQVEW
                     LDDKGVTLVRAHGRLDGERRVRAGDDVLVARKAVIVATGSGVNYPPIDGLADARPWGN
                     REGTAAKEVPGRLAILGGGVIGVEMADAWRSLGAQVTLIEGSDRPLEHEERFAGELVQ
                     EALRVKGVDVRCDAKATAVHRNDDDEVVVSIEGGPDVVADELLVATGRVPHTDDLGVE
                     TVGLEPGRYIDVDDQMRAHGVDGDWLYAIGDVNGRV"
BASE COUNT          344 a          895 c          988 g          350 t
ORIGIN      
        1 ttctcgagcc gcttctcgcg gatcaccccc ggcaggtcca tgtcgaccag gaacagcgtg
       61 aacccgtcgt agcccgcatc cgggtcggtc ttggtgacga gcacgatgaa gtcggcccgg
      121 tggccgttgg tgatgtaggt cttggacccg ttgatcaccc actcgtcgcc gtcgcgcacc
      181 gcgcgggtct tgatgcccga cacgtccgag ccggcgtcgg gctctgtgat gccgaggcag
      241 gagatcttct ccccgcggat ggccggcgcg aggtaggcct gcttctgctc ctcggtgccg
      301 aaggcgagga tcggcggggt cgccatgtcg gtgtggacgg cgatccccat ggccaggccg
      361 ccgcagtcgg cgtggacgat ctcctcggcc agcaccaggt tgcagtagta gtcgccgccc
      421 tgcccgccgt actcctcggg gtaggacagc ccgaggaagc ccagctcgcc catccgctcg
      481 aagacccagt cgtcgaaggt ctcctcctcc cagcgctcgg cgtggggggc cagctccttc
      541 tccacgaacg agccgatcga ctcgcgcagc gcgtcgtgct cctcgttgaa gatgaagtgc
      601 ttcttcgcgg tcccgaactc gggcttgatg acttgatcgg tggcggccat gcggccacgc
      661 tagcaaagta ggaagtggcg ctttctactt ctcagatgtc agcagcggcc cgtcgctgcc
      721 cgccagcagc cgccgcgcga tgccggcctg cgtggcgcgg ccgaaccggt cgtcgctgcc
      781 tccctcggtc agcaccgcga tcgccacccg gcggttcccg cgctccaggt aggccgcctg
      841 gtgctccagc agcgtcccgt gctcgggtcg ccagcccccc ttgaagtaca cgcgccagcg
      901 cgggcgggcg acgcgtggga tgccccaggt ctgcaacggg ctgacggtct ccagcagctc
      961 gcgctcgaac gcccggtggc gcggcggcgt caggtcgtcc agggccagga agaaccgggc
     1021 ctggtcggcg gccgtgacgg ttgctccggc ccagtggccg gcgatcgcga agcggcgcat
     1081 gccggcggcg cgcgccagct cgcgcaccgg cccggcgccg agcgtgcggt agatcgcgtc
     1141 ggccgaggcg ttgtcggaca gccggatcat gtaccccagg gtctcgcgct cggaccgggt
     1201 cgggtcggcg ttccggcgca ggaatgcgac cagcagcatg gccttgctca ggctggccgc
     1261 ggggtagggg cggtcgatgc ccacgccggc cacgccgccg cgctcgtccg ccaccgcgaa
     1321 cgacaccttg cagccgcgcg acctcgcgta gcggctggcc gaacgccggg ccgcgggccc
     1381 cgggaacgcc agggcggacg gagccgtcag ggccgggtag acgcccagcc cggagtcggt
     1441 ctgcgcgggg gcgggagccg ccaggtcacc cagcgggcgc aggcccgggt agacccccat
     1501 ctgggaggtg ccgggcagcg ccggcacggc ggccagcgcg gccacggcga tgaacgggac
     1561 acggcgcacg gagcccggaa cgctacgccg ggcgcgccgg cctcgccggc cgcccttgcg
     1621 ccaggggtaa gactgcatgg catgagcgaa cgcgaattcg acgtggtggt cgtgggtgcc
     1681 gggccggccg gagaggtggc cgccggccgc ctgggcgatg ccggcctgga cgtggcggtg
     1741 gtggagcccg agctgattgg cggcgagtgc tcctactggg cgtgcatgcc gtcgaaggcg
     1801 ttgctccggc cgggtgagct gtacgacgag acgcagcgcg tcccgggcgt ggccgaggcg
     1861 gtggggggcg ggctggacac cgccgccgtg ctcgcgcgcc gcgacgaggt ggtgcacgac
     1921 ttcgacgact cgggccaggt cgagtggctg gacgacaagg gcgtgacgct ggtgcgcgcc
     1981 cacgggcgcc tggacggtga gcgccgcgtg cgcgcgggcg acgacgtgct cgttgcccgc
     2041 aaggccgtga tcgtggccac cggcagcggc gtgaactacc cgccgatcga cggcctggcc
     2101 gacgcccgcc cctggggcaa ccgcgagggc acggccgcga aggaggtgcc cggacggctg
     2161 gccatcctcg gcgggggcgt gatcggcgtg gagatggccg acgcctggcg ctcgctgggc
     2221 gcccaggtca cgctgatcga gggctcggac cggccgctgg agcacgagga gcgcttcgcg
     2281 ggcgagctgg tccaggaggc gctccgggtc aagggcgtgg acgtccgctg cgacgccaag
     2341 gccaccgcgg tccaccgcaa cgacgacgac gaggtcgtgg tctcgatcga gggcgggccc
     2401 gacgtggtgg ccgacgagct gctggtggcc accggccgcg tgccccacac cgacgacttg
     2461 ggcgtcgaga ccgtcggcct cgagcccggc cggtacatcg acgtcgacga ccagatgcgg
     2521 gcccacgggg tcgacggcga ctggctgtac gccatcggcg atgtgaacgg ccgcgtg
//
LOCUS       DASUPW010000457         2404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1351581, whole genome shotgun sequence.
ACCESSION   DASUPW010000457 DASUPW010000000
VERSION     DASUPW010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351581"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VFY44_10280"
     CDS             <1..328
                     /locus_tag="VFY44_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910872.1"
                     /translation="AAHGNHSALNLRYAWPSGDWGSLPIEGGIEAAYRRDLEAAEDPV
                     ALRAEIEERLNAVRSPFRTAEKFGIEEIIDPRDTRRLLCDWAERAHELVSQELGQGPK
                     GRGLRP"
     gene            complement(279..1139)
                     /locus_tag="VFY44_10285"
     CDS             complement(279..1139)
                     /locus_tag="VFY44_10285"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX5910873.1"
                     /translation="MHKPPPHLYFVVSAVFHYLGPSFAVLLFARIQVLGVAWIRIASA
                     AVILWLWRRPRAKLTRTLVAWGTVLAGMNAVFYLAIDRLPLGTVAAIEFLPVIALAAL
                     GARTPRNVAALALAAAGVYLLTDVRLAGEALGFVFAFANAILFALYIVLGHRVSRTEG
                     LDGIDGLALSMIVALVVSTPIGGWQAVPAFTDPVALAAGIGVGICSSVIPYVSDQLAM
                     ARLPRATYSLMVALLPAVATVVGVIVLAQVPTAPEIAGVALVVAAVAVHQERATAEAR
                     APSAPDPAPG"
     gene            1202..1657
                     /locus_tag="VFY44_10290"
     CDS             1202..1657
                     /locus_tag="VFY44_10290"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX5910874.1"
                     /translation="MPTETRQLDAIDRRLLEELQADARLTLAELGRRVGLSSPAVAER
                     LQRLESEGAIRGYHAEVDPRALGYSLTVIIRIRPAPRELAKVADLARATPEVVECQRI
                     TGEDCFFMKAHVRDVLHMEELIDRFNPYGVTTTSIAQSAPVPARPIAVD"
     gene            1689..2015
                     /locus_tag="VFY44_10295"
     CDS             1689..2015
                     /locus_tag="VFY44_10295"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX5910875.1"
                     /translation="MRWVALTGLIFLAGCGGGDMAGDGGAQKVAIEDFKYGPDTIDVK
                     AGDTVDFTNEDGAKHTATSKPQGTFDSGDIAQGQTRPVTFRKAGSFRLYCVYHPTMAA
                     RVTVTK"
     gene            2031..>2404
                     /locus_tag="VFY44_10300"
     CDS             2031..>2404
                     /locus_tag="VFY44_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910876.1"
                     /translation="MGARASAALAMVAATLFVAVPASAAPPSSCGLPGPDQATTGGFD
                     AARQGSFVFVPFDVPAGTTQVRVAYCWDGSQGTSDHTLDLGVYQARGDARRPWGGANF
                     RGWGGSGYRDVTITPQGFGTDA"
BASE COUNT          376 a          863 c          876 g          289 t
ORIGIN      
        1 ggccgcgcac ggcaaccact cggcgctgaa cctgcgctac gcctggccgt cgggcgactg
       61 gggctcgctg cccatcgagg gcggcatcga ggccgcctat cgccgcgacc tggaggcggc
      121 cgaggacccg gtggcgctac gtgccgagat cgaggagcgc ctgaacgccg tccgctcgcc
      181 cttccgcacg gccgagaagt tcgggatcga ggagatcatc gacccgcgcg acacccgccg
      241 cctgctgtgc gactgggccg agcgcgcgca cgagctggtc agccaggagc tgggtcaggg
      301 gccgaagggg cgcgggcttc ggccgtagcg cgctcctggt ggaccgccac ggcggccacc
      361 accagcgcca cccccgcgat ctccggcgcc gtgggcacct gggcaaggac gatcacgccg
      421 accaccgtgg ccaccgcggg cagcagcgcc accatcagcg agtacgtggc gcgcggcagc
      481 cgcgccatgg ccagctggtc ggacacgtac gggatcaccg acgagcagat gccgacgccg
      541 atgccggccg ccagcgcgac cgggtcggtg aacgcgggca ccgcctgcca ccccccgatg
      601 ggcgtggaga ccaccagggc gacgatcatc gacagcgcca ggccgtcgat gccgtccagg
      661 ccctcggtcc gcgacacgcg gtgcccgagc acgatgtaga gcgcgaacag gatcgcgttc
      721 gcgaacgcga acacgaagcc cagcgcctcg ccggccagcc tcacgtcggt cagcaggtag
      781 accccggccg cggcgagcgc cagcgccgcc acgttccggg gcgtgcgcgc gcccagcgcc
      841 gccagcgcga tcaccggcag gaactcgatg gccgccaccg tgcccagggg gaggcggtcg
      901 atggccaggt agaagaccgc gttcatgccg gccaggacgg tcccccaggc caccagggtg
      961 cgcgtgagct tcgcccgcgg gcgccgccac agccacagga tcaccgcggc cgaggcgatc
     1021 cggatccacg ccacccccag gacctggatc cgggcgaaca gcagcacggc gaacgacggc
     1081 cccaggtagt ggaagaccgc gctgaccacg aagtagaggt ggggaggggg cttgtgcacc
     1141 cctgtagcct cgacggcgag agcccgtgat cacagggtca ttcgacggat ctgagttcgt
     1201 tatgcctacg gaaacgcgac aactggacgc aatcgaccgt cgattgctcg aggagctcca
     1261 ggcggacgcc cgcttgacgc tggccgagct gggccgccgc gtcggcctgt cctcgcccgc
     1321 ggtcgcggag cggctgcagc gccttgagtc ggagggtgcg atccgcgggt accacgccga
     1381 ggtcgacccg cgcgccctgg gctactcgct gacggtgatc atccgcatcc gcccggcgcc
     1441 gcgggagctg gccaaggtgg ccgacctggc gcgggcgacc cccgaggtcg tggagtgcca
     1501 gcgcatcacc ggcgaggact gcttcttcat gaaggcgcac gtgcgggacg tgctgcacat
     1561 ggaggagctg atcgaccggt tcaacccgta cggggtgacc accacgtcca tcgcccagtc
     1621 ggcgccggtg ccggcgcgcc cgatcgccgt ggactgatcc gaggcggccc gggcggcgca
     1681 gacctggcat gcgctgggtg gcattgaccg ggttgatctt cctggccggt tgtggcggcg
     1741 gcgacatggc cggcgacggc ggcgctcaga aggtcgcgat cgaggacttc aagtacgggc
     1801 cggacacgat cgacgtgaag gcgggggaca cggtcgactt caccaacgag gacggcgcca
     1861 agcacacggc cacgtccaag ccgcagggca ccttcgactc gggcgacatc gcgcagggcc
     1921 agaccaggcc cgtgaccttc aggaaggccg gcagcttccg cctgtactgc gtctaccacc
     1981 ccacgatggc cgcgcgggtg acggtcacga agtagccgta gactggccgc gtgggagccc
     2041 gggcgtcggc ggcgctggcg atggtggcgg cgacgttgtt cgtcgccgtc ccggcaagcg
     2101 cggcgccgcc gtcgagctgc ggcctcccgg gtcccgatca ggcgaccacc ggcggcttcg
     2161 acgccgcccg ccagggcagc ttcgtgttcg tgcccttcga cgtgcccgcc ggcaccacgc
     2221 aggtgcgcgt ggcctactgc tgggacggct cccagggcac cagcgatcac acgcttgacc
     2281 tcggcgtcta ccaggcgcgc ggcgacgccc ggcgaccgtg gggcggggcg aacttccgcg
     2341 gctggggcgg gtccggatac cgcgacgtca cgatcacccc gcagggcttc gggaccgatg
     2401 cgca
//
LOCUS       DASUPW010000458         3320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_384200, whole genome shotgun sequence.
ACCESSION   DASUPW010000458 DASUPW010000000
VERSION     DASUPW010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384200"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..861)
                     /gene="pheA"
                     /locus_tag="VFY44_10305"
     CDS             complement(7..861)
                     /gene="pheA"
                     /locus_tag="VFY44_10305"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEX5910877.1"
                     /translation="MNSVAYLGPPGTHTEEALLASAPGPVEREPRGTLLETVMAVQQG
                     ETDRAVVPIENSLEGGVAATLDALAGPADGVRIVSEVVHQIHHNLIARPGTALGDVRK
                     VLSIPHATGQCVRFLDANLPDAERLAVASTAEAVRVVGDSDEPWAALGSALAAELYGC
                     EVLAANVEDRDDNQTRFVWLAQAAEAEAPTEGAATTSVVFWGFNDESPGALVSVLGEL
                     SSRDINLTRIESRPRRVRLGHYMFFADLDGSTADPHVAEALAALEARVHELRLLGSYP
                     SGPGRAPR"
     gene            complement(858..1382)
                     /locus_tag="VFY44_10310"
     CDS             complement(858..1382)
                     /locus_tag="VFY44_10310"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HEX5910878.1"
                     /translation="MDGLNSTEGIVALAGAALGLIALVLAIVLAVKLRRLRAAQRRVL
                     GDAGELDLVEHAARLEQGFVALRDWVEETAQGIETRMGSAEDRIDGCVTYRSLIRYDA
                     YGEMSGRQSSSIALLDSHRSGVVMSSILHRENARVYVKQLHEGSSELELSPEEQEAVT
                     AALERRPPEPVDHT"
     gene            complement(1393..2379)
                     /locus_tag="VFY44_10315"
     CDS             complement(1393..2379)
                     /locus_tag="VFY44_10315"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HEX5910879.1"
                     /translation="MVAAGVGIVVLLLVVLLFRGCLSSRKENAMREYVQDVTGLVRES
                     DQESRNLFRTLAAPANAQDVEIQNRLNTLRAQGEQILDRTEALDTPDELKGAQSYLTE
                     VLKFRRDGVAAIARDLPRASADQGDRRRGIEQVAASMQNFLASDVIYQTRFTPSLRGA
                     LDDEGLGGEQIPRSRFLPEVDWVLPATVAEKVPKLGGSGASDEEAAPGLHGTGVASAT
                     LGGQTLNPGGQAANIPLSDDLTMTVQIANQGENTEEDVRVEVTIGRGGDAVRAEKTLD
                     TIAAGETKPVEIPITDPPPTGQNVPITVSVAKVPGEEKLDNNKASYSAIFTR"
     gene            2601..>3320
                     /locus_tag="VFY44_10320"
     CDS             2601..>3320
                     /locus_tag="VFY44_10320"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5910880.1"
                     /translation="MRRSETTTIPEGEPTVRLGPDRVAGRVALDRYRLDERLGAGGFG
                     VVWRAHDLKLERDVAVKAVPRTGGEAEQSRTEREALAAARLNHPGIVALYEFGYDDEY
                     IYLVSELVEGATLGELTAGGVLSDRDVARIGQALGEALQHAHARGVIHRDVKPANVMV
                     LAEPAAGAGFAKLTDLGVAHVASADDLTATGDVIGTLAYMAPEQAEGQRITAACDVYS
                     LALTLYEAWTGTNPVRARGAAA"
BASE COUNT          430 a         1181 c         1231 g          478 t
ORIGIN      
        1 gcgattctag cgaggggccc ggcccggccc gctggggtag gagcccagga gccgaagctc
       61 gtgcacgcgc gcctccagcg cggccagtgc ctcggccacg tgcgggtcgg cggtcgaccc
      121 gtccaggtcg gcgaagaaca tgtagtggcc cagccgcacc cggcgcgggc gcgactcgat
      181 ccgggtcagg ttgatgtcgc ggctggagag ctcgcccagc acggacacga gcgcaccggg
      241 ggactcgtcg ttgaagcccc agaacacgac cgatgtcgtg gccgcgcctt cggtcggcgc
      301 ctcggcctcg gccgcttgcg ccagccagac gaagcgggtc tggttgtcgt cgcggtcctc
      361 cacgttggcg gccaggacct cgcacccgta cagctcggcc gccagcgccg agcccagcgc
      421 ggcccacggc tcgtccgagt cgccgaccac ccggacggcc tcggcggtgg aggccaccgc
      481 cagccgctcc gcgtcgggca ggttggcgtc caggaagcgc acgcactgcc cggtggcgtg
      541 ggggatggac agcaccttgc gcacgtcgcc gagcgccgtc ccggggcggg cgatcaggtt
      601 gtggtggatc tggtgcacca cctcggacac gatccggacg ccgtccgcgg ggccggcgag
      661 ggcgtcgagc gtggcggcga ctccgccttc gagcgagttc tcgatcggga cgacggcgcg
      721 gtcggtctcg ccctgctgga ccgccatgac ggtctccagc agcgtcccgc gcggctcgcg
      781 ctcgaccggg ccgggcgcgg aggccagcag cgcctcctcg gtgtgcgtgc cgggcggccc
      841 gaggtaggcc accgagttca cgtgtggtcg accggctccg gcggacgccg ctcgagggcc
      901 gcggtcacgg cctcctgctc ctcgggcgag agctcgagct cggagctgcc ctcgtgcagc
      961 tgcttgacgt acacgcgcgc gttctcgcgg tgcaggatcg aggacatgac caccccggac
     1021 cggtgcgagt ccagcagcgc gatcgagctg gactggcggc cggacatctc gccgtaggcg
     1081 tcgtagcgga tcagcgagcg gtaggtcacg cagccgtcga tccggtcctc ggccgacccc
     1141 atccgggtct cgatgccctg tgcggtctcc tcgacccagt cgcgcagggc gacgaagccc
     1201 tgctccaggc gcgcggcgtg ctccacgaga tccagctcgc cggcgtcgcc gagcacgcgt
     1261 cgttgcgcgg cgcgcaggcg gcgcagcttg acggcgagca cgatcgccag caccagggcg
     1321 atcaggccca gcgcggcgcc cgcgagggcg acgatgccct ccgttgagtt gagtccgtcc
     1381 acgcgggtga aactaccgcg tgaagatcgc ggagtacgac gccttgttgt tgtcgagctt
     1441 ctcttcgccc ggaaccttgg ccacggacac cgtgatcggc acgttctggc ccgtcggcgg
     1501 cgggtcggtg atggggatct cgaccggctt cgtctcgccc gcggcgatcg tgtcgagcgt
     1561 cttctcggcc cgcaccgcgt cgccgccacg gccgatcgtg acctccacgc ggacgtcctc
     1621 ctcggtgttc tcgccctggt tggcgatctg gacggtcatc gtgaggtcgt cggagagggg
     1681 gatgttcgcg gcctgcccac cggggttcag tgtctggccg cccagcgtgg ccgaggcgac
     1741 gccggtgccg tgcaggccgg gcgcggcctc ctcgtcgctg gcgccgctgc ccccgagctt
     1801 cgggaccttc tcggccacgg tggcgggcag gacccagtcg acctcgggca ggaagcggct
     1861 gcgcgggatc tgctccccgc ccaggccctc gtcgtccagc gcgccgcgga gcgagggcgt
     1921 gaagcgggtc tggtagatca cgtcgctggc caggaagttc tgcatgctgg cggccacctg
     1981 ctcgataccc cgccggcggt cgccctggtc ggcgctggcc cgggggaggt cgcgggcgat
     2041 ggccgcgacc ccgtcgcggc ggaacttcag cacctcggtc aggtagctct gggcaccctt
     2101 gagctcgtcg ggggtgtcca gggcctcggt gcggtccagg atctgctcac cctgggcgcg
     2161 gagggtgttc agccggttct ggatctccac gtcctgggcg ttggcggggg cggcaagggt
     2221 ccggaacagg ttcctgctct cctggtcgga ctcgcgtacc agccccgtga cgtcctggac
     2281 gtactcgcgc atggcgttct ccttgcggga gctcaggcag ccgcggaaga gcagcaccac
     2341 cagcaacagc acgacgattc ccaccccggc ggcgaccaat cgccttacca tgagggtttg
     2401 gcgatcggtg gatggacccg ccggaggcgg gcgccgcccc gatcgggggc gcggttgtgg
     2461 ctgatccggt tcgtcgaaga aggacagggt ctcgcgcctc gcttccggtg aggagtatga
     2521 cctttccatc ggttgtcctg cctcggagcc gtcccgaggc cggaaggggg aaggtcgcgg
     2581 cggcagaact acgaatccag atgagaaggt ccgagaccac cacgatcccc gagggcgagc
     2641 ccaccgttcg cctcggcccc gaccgcgtcg cgggccgcgt ggcgctggac cggtaccggc
     2701 tggacgagcg cctcggcgcc ggcggcttcg gggtggtctg gcgcgcccac gacctcaagc
     2761 tggagcgcga cgtggcggtc aaggcggtcc cgcgcaccgg cggcgaggcc gagcagtcgc
     2821 gcaccgagcg cgaggccctg gccgccgcgc gcctgaacca cccggggatc gtggccctgt
     2881 acgagttcgg ctacgacgac gagtacatct acctggtctc cgagctggtc gagggcgcca
     2941 cgctcggtga gctgaccgcg ggcggcgtgc tgtcggaccg cgacgtggcc cgcatcggcc
     3001 aggccctggg cgaggcgctc cagcacgcgc acgcccgcgg cgtgatccac cgcgacgtca
     3061 agccggcgaa cgtgatggtg ctggccgagc ccgcggccgg cgccggcttc gccaagctga
     3121 ccgaccttgg cgtggcccac gtggccagcg ccgacgacct gaccgccacc ggcgacgtga
     3181 tcggcacgct ggcctacatg gccccggagc aggccgaggg gcagcggatc accgcggcct
     3241 gcgacgtcta ctcgctggcc ctgacgctgt acgaggcctg gaccggcacg aacccggtgc
     3301 gcgcccgggg cgccgccgcg
//
LOCUS       DASUPW010000459         2848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_105753, whole genome shotgun sequence.
ACCESSION   DASUPW010000459 DASUPW010000000
VERSION     DASUPW010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105753"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VFY44_10325"
     CDS             complement(<1..446)
                     /locus_tag="VFY44_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910881.1"
                     /translation="MRSRRLSFRSRVLIGLALAAGPVVVVIALLLRDEPAPQKPPPPA
                     IHAGQVKYRTIYHSSQTPGYTAWVGAWLMPDDSLMTGFVEATGPMNPAERAQTPASVL
                     ELFGTPRAEDPQRDFWGLDLSTRFLRSSDGGRRWRLDSSERFKAIGP"
     gene            complement(472..1932)
                     /locus_tag="VFY44_10330"
     CDS             complement(472..1932)
                     /locus_tag="VFY44_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910882.1"
                     /translation="MSPSSGNQNGLPASPDVAAGAAGPGRLQRLGQAVRQHPIAVLLV
                     LIALPAVGVAIALARGNDHEASTTLLLGPPAAAQQADRGATLDERAVATARELADSPA
                     VAARAAGSLGGTTPGEIADAVEVDQNGDANALTITASGESPAQAVRRADAYAEAYSAV
                     AGEVQPASLRPAAASQLSRATPSPDPDSPVVAYGLIGLGAAILLVLLGAPLARGLDWR
                     IKDVAELEEIYGLPALAHIPRSRSLAKRGRSARAEATKNTVGFSEEAEAFRTLRTNLR
                     YFNVDAPVRSILVASPLPGDGKSTVARHLAITMASMGDSICLVNADLRKQEPGVLPTP
                     DGLSLVLAGFDPDTALTEIPIAYDPLSEESRVLVELPSGPLPPNPAELLESDRMQWLV
                     RQLERRFDLVIIDSPALVTVSDALTLVPRVSGVLAVGGIGQTTRQAAVDLRKQIELLG
                     GRSLGVVANFSNQGRRYYGYYEKEQEAKAQASAAGR"
     gene            complement(1988..>2848)
                     /locus_tag="VFY44_10335"
     CDS             complement(1988..>2848)
                     /locus_tag="VFY44_10335"
                     /inference="COORDINATES: protein motif:HMM:NF027579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HEX5910883.1"
                     /translation="YEVHIDADLVEGELELGEVTVPGRSEQEVLLSTYLCHPSMANNE
                     LSGPMVVAQLADLLRNRPEPPRFTYRLLFVPETIGAIAYLARFGQRLRERLAAGYVVT
                     CIGDPGPFTYKPSRRGGTLADRAALHVLRHAGHEYEQVDFYPPASDERQYCSPGFDLP
                     VGSLMRTPYFEYPEYHTSLDDLELVTPDSLGQSLRMYHRVVEALEAAATYRVTVAEGE
                     PRLGPRGLYATTGGSKVNDQAKRDMMFLLNYCDGRPDLLAAADRADRPIWEIEPTVRR
                     LLDHDLLRPA"
BASE COUNT          375 a         1014 c         1084 g          375 t
ORIGIN      
        1 ggcccgatgg ccttgaaccg ctccgagctg tccagcctcc agcgccggcc gccgtcgctg
       61 gagcgcagga agcgcgtcga caggtccagg ccccagaagt cgcgctgggg gtcctcggcc
      121 cgcggcgtgc cgaacagctc caggaccgac gccggggtct gggcccgttc ggccgggttc
      181 atcggcccgg tggcctcgac gaagccggtc atgagcgagt cgtcgggcat gagccaggcg
      241 cccacccagg cggtgtaccc cggcgtctgg gaggagtggt agatcgtccg gtacttgacc
      301 tgcccggcgt ggatggccgg cgggggcggc ttctgcggcg cgggctcgtc gcgcagcagc
      361 agcgcgatca cgacgaccac cgggcccgcc gcgagcgcga ggccgatcag gacccggctg
      421 cgaaaggaga ggcgcctcga ccgcacggga gggactgtag ggagcggggt gtcagcgacc
      481 cgcggctgac gcctgggcct tggcttcctg ttccttctcg tagtagccgt agtagcgccg
      541 gccctggttc gagaagttgg cgaccacgcc cagcgagcgg ccgccgagga gctcgatctg
      601 cttgcgcagg tccaccgcgg cctggcgcgt ggtctgcccg atcccgccca ccgccagcac
      661 gcccgagacg cgcgggacca gcgtcagcgc gtcgctgacg gtcaccagcg ccgggctgtc
      721 gatgatcacc aggtcgaacc gccgctccag ctggcgcacc agccactgca tgcggtcgct
      781 ctccagcagc tcggcggggt tgggcggcaa cggcccgctg ggcagctcga ccagcacccg
      841 cgactcctcc gacagcgggt cgtaggcgat cgggatctcg gtcagggcgg tgtcggggtc
      901 gaagccggcc agcaccagcg acaggccgtc gggggtggga agcacccccg gctcctgctt
      961 gcgcaggtcg gcgttgacca ggcagatcga gtcccccatc gaggccatgg tgatggccag
     1021 gtggcgggcc accgtggact tgccgtcgcc cggcagcggg ctggccacca ggatcgaccg
     1081 caccggggcg tcgacgttga agtagcgcag gttggtgcgc agcgtgcgga aggcctcggc
     1141 ctcctcgctg aagccgacgg tgttcttggt ggcctcggcg cgggcggacc ggccccgctt
     1201 ggccagcgac cggctgcgcg gtatgtgggc cagggccggg aggccgtaga tctcctccag
     1261 ctcggccacg tccttgatcc gccagtccag gccgcgcgcc agcggcgcgc ccagcagcac
     1321 cagcaggatc gcggcgccga gcccgatgag cccgtaggcc accacggggc tgtcgggatc
     1381 cggcgacggc gtggcccgcg acagctgcga cgccgcggcc gggcgcagcg aagcgggctg
     1441 gacctccccc gcgacggccg agtaggcctc ggcgtaggcg tcggcgcggc gcacggcctg
     1501 ggcgggcgac tccccgctgg cggtgatggt cagggcgttg gcgtcgccgt tctggtccac
     1561 ctccacggca tcggcgatct cgccgggggt ggtccccccg agcgaacccg ccgcacgagc
     1621 cgcgacggcc gggctgtcgg ccagctcacg cgccgtcgcc acggcgcgct cgtccagcgt
     1681 ggctccgcgg tcggcctgct gggccgcggc gggcggcccc agcagcagcg tggtcgacgc
     1741 ctcgtggtcg ttcccgcgcg cgagggcgat ggccacgccc acggcgggaa gggcgatcag
     1801 caccagcagc accgcgatcg ggtgctggcg caccgcctgg cccaggcgct ggaggcggcc
     1861 cgggcctgcg gcgcccgcgg ccacatccgg tgacgcgggc agcccgttct ggttgccgga
     1921 agaagggctc atggagcgga cgccagcgta acccgtcccc cggcgggccg ccaccgcgcg
     1981 gggcggctca cgccggccgg agcaggtcgt ggtcgagcag gcgcctgacg gtgggctcga
     2041 tctcccagat cggccggtcg gcgcggtcgg cggccgccag caggtcgggg cggccgtcgc
     2101 agtagttcag gaggaacatc atgtcccgct tggcctggtc gttgaccttc gagccgcccg
     2161 tggtggcgta caggccccgc gggcccagcc ggggctcgcc ctccgcgacg gtcacgcggt
     2221 acgtggccgc cgcctccagg gcctcgacca cgcggtggta catccgcagg ctctgaccca
     2281 gcgagtccgg cgtgaccagc tccagatcgt ccagcgaggt gtggtactcg gggtactcga
     2341 agtacggcgt gcgcatgagc gagcccacgg gcaggtcgaa gcccggtgag cagtactggc
     2401 gctcgtcgct ggccggcggg tagaagtcga cctgctcgta ctcgtgcccg gcgtgccgga
     2461 ggacgtggag cgcggcgcgg tcggccagcg tgccgccgcg gcgtgagggc ttgtacgtga
     2521 acggccccgg gtcgccgatg cacgtgacca cgtatccggc ggccagccgc tcgcggagcc
     2581 gctgcccgaa acgggccagg tacgcgatgg cgccgatcgt ctccggcacg aacagcagcc
     2641 ggtaggtgaa ccggggcggt tccgggcggt tgcggagcag gtcggccagc tgggccacaa
     2701 ccatcgggcc cgacagctcg ttgttggcca tcgaggggtg gcacaggtac gtggacagca
     2761 ggacctcctg ctcgctgcgg cccggcacgg tcacctctcc aagctccagc tccccctcca
     2821 ccaggtcggc gtcgatgtgc acctcgta
//
LOCUS       DASUPW010000460         2284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_385362, whole genome shotgun sequence.
ACCESSION   DASUPW010000460 DASUPW010000000
VERSION     DASUPW010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385362"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VFY44_10340"
     CDS             complement(<1..383)
                     /locus_tag="VFY44_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX5910884.1"
                     /translation="MRVRALEAELERERAEGRERERQMERYASDLNETFRAERRRADE
                     LSDSYVATVRALTNAVEARDAYTGKHAERVAAYGLEMARRISDTLAADPQTEFGFLLH
                     DVGKVAIPDVVLHKAGDLSPDEEAL"
     gene            complement(434..>2284)
                     /locus_tag="VFY44_10345"
     CDS             complement(434..>2284)
                     /locus_tag="VFY44_10345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5910885.1"
                     /translation="QATRRLAEGDLDQRVEPGGPREIQELDSSFNEMAEQLQQAQIRL
                     EAERASLALTIESLGDALVVTDEQGIVTAANPRAAELVPGLTPGADAYAGGSPLPEPE
                     LGLAGDVAIEVDGRSLAATAGRLPAARGAGLVWTIRDVSERARLERMKSDFVATASHE
                     LRSPLTSIKGFVELLGRSEALGDREREFVDVVLQSTDRLVELVNDLLDVARLEAGRME
                     IHARLFDLDPVIEEVAVLMGPRVAEKDQQLVLDLPPGLPKVLADPVRVRQVLVNLISN
                     AHQYTEEGGTITVIADGEGQDVALSVADDGQGMAQEDLDHVFDRFVRRDDTGAGTGLG
                     LSIVRSLVDLQGGTIDVQSQVGEGTVFTVRLPAEGLGSQTSHRAVEGSLVLVLTPRAD
                     LAGTLMERVEAGGAKAKAPVTVRPGLADLRSQRYDVLLLDLEGLGEDGMELLSRLRDD
                     PELGRRPLVTVCSERQDQALAGEWRVRADVDADELAGTLGAAIVAERSSILVVGRSAV
                     RDRVEAELLATGLDHQWATTGTAAAQACRGERFEVALVDARMRAFDEVLDALDLRGKR
                     HDKAVIVFTDEDTDQEVDHPSVRAVVPLEEAASAAVEALADAPTASLDQG"
BASE COUNT          281 a          878 c          804 g          321 t
ORIGIN      
        1 atcagcgcct cctcgtcggg cgacaggtcg ccggccttgt gcagcaccac gtcgggaatg
       61 gccaccttgc ccacgtcgtg gagcaggaag ccgaactcgg tctggggatc ggcggccagc
      121 gtgtccgaga tccgccgcgc catctccagg ccgtaggcgg ccacccgctc ggcgtgcttg
      181 ccggtgtagg cgtcgcgggc ctcgaccgcg ttggtcagcg ccctgaccgt ggccacgtac
      241 gagtcgctca gctcgtcggc gcggcggcgc tcggcccgga aggtctcgtt caggtcggag
      301 gcgtagcgct ccatctggcg ctcgcgctcg cggccctcgg cgcgctcgcg ctcgagctcg
      361 gcctccagcg cgcgcaccct caccggatca ccgccgctgg tcgaagcctc ttcggaaagc
      421 gccatcccca tatctacccc tgatccaggc tagctgttgg cgcgtcggcg agcgcctcca
      481 ccgcggccga cgcggcctct tcgagcggaa cgaccgcgcg cacggagggg tgatcgacct
      541 cctggtccgt gtcctcgtcg gtgaacacga tgaccgcctt gtcgtggcgt ttcccgcgca
      601 ggtcgagggc gtcgagcacc tcgtcgaagg cgcgcatccg ggcgtccacc agcgcgacct
      661 cgaagcgctc gccgcggcac gcctgggcgg ccgcggtccc ggtggtggcc cactggtggt
      721 ccaggcccgt ggccagcagc tcggcctcga cccggtcgcg gacggcggag cgaccgacca
      781 ccaggatgct cgagcgctcg gccacgatgg cagcgcccag cgtgccggcc agctcgtcgg
      841 cgtccacgtc ggcccggacg cgccactcgc cggccagggc ctggtcctgg cgctcgctgc
      901 acaccgtgac cagcggccgc cggcccagct cggggtcgtc gcgcaggcgg gacagcagct
      961 ccatgccgtc ctcgcccagg ccctcgaggt ccagcagcag cacgtcgtag cgctgggagc
     1021 gcaggtcggc caggcccggg cgcaccgtga ccggcgcctt ggccttcgcg ccgccggcct
     1081 cgacgcgctc catcagcgtt cccgccaggt cggcccgggg cgtcagcacc aggaccaggc
     1141 tgccctcgac ggcgcggtgg gaggtctgcg accccaggcc ctcggccggc aggcggaccg
     1201 tgaacaccgt gccctcgccc acctggctct ggacgtcgat ggtgccgccc tggaggtcga
     1261 cgagcgagcg cacgatcgac aggcccagcc cggtgccggc gccggtgtcg tcgcggcgca
     1321 cgaagcggtc gaagacgtgg tccaggtcct cctgcgccat gccctggccg tcgtcggcca
     1381 ccgacagcgc gacgtcctgg ccctcaccgt cggcgatcac cgtgatcgtc ccgccctcct
     1441 cggtgtactg gtgcgcgttg gagatcaggt tcaccagcac ctggcggacg cggacggggt
     1501 cggccaggac cttgggcagg cctggcggca ggtccagcac cagctgctgg tccttctcag
     1561 caacgcgcgg ccccatcagc accgccacct cctcgatcac ggggtcgagg tcgaacaggc
     1621 gcgcgtggat ctccatccgg ccggcctcca gccgcgccac gtcgagcagg tcgttcacga
     1681 gctcgacgag ccggtcggtg ctctgcagca ccacgtcgac gaactcgcgc tcgcggtcgc
     1741 ccagcgcctc cgaccgcccg agcagctcca cgaagccctt gatcgaggtc agcgggctgc
     1801 gcagctcgtg cgaggcggtg gccacgaagt cgctcttcat gcgctccagg cgcgcccgct
     1861 cggacacgtc gcggatggtc cacaccagcc ccgcgccgcg ggcggcgggg aggcggccgg
     1921 cggtcgccgc cagcgatcgg ccgtccacct cgatcgccac gtcgccggcc agccccagct
     1981 ccggctcggg cagcgggctg ccgcccgcgt aggcgtcggc cccgggggtc aggccgggca
     2041 ccagctccgc ggcgcgcggg ttggccgcgg tcacgatgcc ctgttcgtcg gtgaccacaa
     2101 gcgcgtcccc gaggctctcg atggtcagcg cgaggctggc ccgctctgcc tcgaggcgga
     2161 tctgcgcctg ctggagctgc tcggccatct cgttgaagga ggagtccagc tcctggatct
     2221 cgcgcgggcc gccaggctcg acgcgttggt ccaggtcgcc ctcggccagc ctgcgcgtgg
     2281 cctg
//
LOCUS       DASUPW010000461         2769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_799605, whole genome shotgun sequence.
ACCESSION   DASUPW010000461 DASUPW010000000
VERSION     DASUPW010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799605"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VFY44_10350"
     CDS             <1..928
                     /locus_tag="VFY44_10350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX5910886.1"
                     /translation="ALHAGGVEVIGIRVPRGAPTAERAGLIAGSVLGLYPHRTRDGSK
                     FTSGVVVVAGGGEGLTGAPTMTALAAQRAGAGYVQVAVPKAAQTTLELRLLEAMTRGL
                     PDRDGGHTEEGVAILAEMAERAGAVVLGPGLGRTEGAQDFARGVAQAVQAPLLIDADG
                     LNAHAGALEDLAGRGGATVLTPHEAELGRLLGREPGDIAADRLAAVREAAATSGAVTL
                     LKGDDTIVARPDGLAAVSRGGTPALATAGTGDVLSGLIGALLAKGVDPFAAAALGTLA
                     HANAGRGAAERHGADHVVAGDVIDALPSAFGE"
     gene            940..1401
                     /locus_tag="VFY44_10355"
     CDS             940..1401
                     /locus_tag="VFY44_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5910887.1"
                     /translation="MASTVGEIMDGNPETVTADTPVEEVIRIMQTNELPGIPVVNEGG
                     RCVGIVTETDMVLPDDDGDLHLPHYVNIFGGTVFLEPLRKWEDRLKKAFASTAKDMMS
                     ENPVTVEPSTTIDEAAGIIHDTGHNRLPVVEHGRLVGVVTRVDVLGGLVSD"
     gene            1403..2497
                     /gene="alr"
                     /locus_tag="VFY44_10360"
     CDS             1403..2497
                     /gene="alr"
                     /locus_tag="VFY44_10360"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX5910888.1"
                     /translation="MAQRALARVHTAAIQANCRLLAERVGGDTALCAVVKADGYGHGA
                     ATAARAALAGGAEWLAVATAVEAAALRAEGLVVPVLVMGALSADEADLALDNDADVVV
                     WTRGFLESIASRRPRVHVKLDTGMGRLGTADVEEARALVRAVDLDHDAGLVGVMTHFA
                     TADEPGDDHFPLQLERFTAFADEVRRDHPGVIAHAANSAATLRDKASHFDMVRCGIAV
                     YGMDPFQQDAAAQGLAPALELHSYVAAVKRFEAGDSAGYGRRWAADRATWVATLPIGY
                     GDGWRRGLTNDCDVLIGGRRYPLVGTVSMDNVTVDLGPETDVEVGAAAVLIGSQGDEA
                     VTAEEIARRLGTINYEVTCGLSARVPRLEV"
     gene            2500..>2769
                     /locus_tag="VFY44_10365"
     CDS             2500..>2769
                     /locus_tag="VFY44_10365"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910889.1"
                     /translation="MPATALETAEPVKAVRQALAGGPDARLVGGVVRDAHLGRPLGDL
                     DLAVDGDPAQVARTVAKALGGPVFPLSEAFGSWRALDGDRRFEVDV"
BASE COUNT          354 a          997 c         1094 g          324 t
ORIGIN      
        1 ggcgctgcac gccggcggcg tcgaggtcat cggcattcgc gtaccccgcg gcgcgcccac
       61 ggcggagcgg gcgggcctga tcgccggctc cgtcttgggc ctgtacccgc accgcacccg
      121 cgacgggtcg aagttcacct ccggcgtggt ggtggtcgcg ggtgggggcg agggcctgac
      181 cggagcgccc acgatgacgg cgctggcggc ccagcgcgcg ggcgcgggct acgtgcaggt
      241 ggcggtcccc aaggcggcgc agacgaccct ggagctgcgc ctgctggagg ccatgacgcg
      301 cgggctgccc gaccgcgacg gcggtcacac ggaggagggg gtcgccatcc tggccgagat
      361 ggccgagcgg gccggcgcgg tggtcctggg accgggcctg ggtcgcacgg agggcgcgca
      421 ggacttcgcc cgcggggtcg cccaggccgt tcaggcgccg ctcctgatcg acgccgacgg
      481 cctgaacgcc cacgccggcg cgctggagga cctggccggg cgcggcggcg ccacggtgct
      541 gacgccgcac gaggccgagc tgggccgcct gctggggcgc gagcccggcg acatcgccgc
      601 cgaccgcctg gcggcggtcc gggaggcggc cgccaccagc ggcgcggtca ccctgctcaa
      661 gggcgacgac accatcgtgg cccgccccga cgggctcgcg gcggtcagcc gcggcggcac
      721 gccggcgctg gccacggcgg gcacggggga cgtgctctcg ggcctgatcg gcgcgctgct
      781 ggccaagggc gtggacccgt tcgcagccgc tgcgctgggc acgctggccc acgcgaacgc
      841 cggccgcggg gccgccgagc gccacggcgc cgaccacgtg gtggccggag acgtgatcga
      901 tgcgctgccg tccgcgttcg gcgagtaatc tcacgcgcca tggcgagcac ggtcggagag
      961 atcatggacg gcaacccgga gacggtgacc gcggacaccc ccgtcgagga ggtcatccgc
     1021 atcatgcaga ccaacgagct gcccgggatc ccggtggtca acgagggcgg ccgctgcgtg
     1081 ggcatcgtga ccgagaccga catggtcctg cccgacgacg acggcgacct gcacctgccc
     1141 cactacgtga acatcttcgg cggcacggtc ttcctggagc cgctgcgcaa gtgggaggat
     1201 cgcctgaaga aggcgttcgc ctcgacggcc aaggacatga tgagcgagaa ccccgtcacg
     1261 gtggagccct cgacgaccat cgacgaagcc gccgggatca tccacgacac cggccacaac
     1321 cggctgccgg tggtcgagca cggccgcctg gtcggcgtgg tcacgcgcgt cgacgtgctc
     1381 ggcggcctcg tcagcgactg acatggccca gcgcgcgctc gcgcgggtcc acaccgccgc
     1441 catccaggcc aactgccgcc tgctggccga gcgcgtgggg ggtgacaccg ccctgtgcgc
     1501 cgtcgtcaag gccgacgggt acggccacgg ggccgccacc gcggcgcgcg cggccctggc
     1561 cggcggcgcc gagtggctgg cggtggccac cgcggtggag gcggcggcgc tgcgggccga
     1621 ggggctggtg gtcccggtcc tggtcatggg cgcgctctcg gccgacgagg ccgacctggc
     1681 gctggacaac gacgccgacg tggtggtgtg gacgcggggg ttcctggagt cgatcgcctc
     1741 gcgccgcccg cgggtgcacg tgaagctgga caccggcatg ggccgcctgg gcaccgccga
     1801 cgtggaggaa gcgcgcgctc tggtgcgcgc cgtcgacctg gaccacgacg ccgggctggt
     1861 gggcgtgatg acccacttcg ccaccgccga cgagccgggc gacgaccact tcccgctcca
     1921 gctggagcgg ttcacggcgt tcgcggacga ggtgcggcgc gaccaccccg gcgtgatcgc
     1981 ccacgccgcc aacagcgccg ccacgctgcg cgacaaggcc tcccacttcg acatggtccg
     2041 ctgcggcatc gccgtgtacg gcatggaccc gttccagcag gacgcggccg cccagggcct
     2101 ggcgccggcg ctggagctgc acagctacgt ggccgccgtg aagcggttcg aggccggtga
     2161 cagcgcgggc tatggccgcc gctgggccgc cgaccgcgcc acctgggtgg ccacgctgcc
     2221 gatcggctac ggcgacggct ggcggcgcgg gctgaccaat gactgcgacg tcctgatcgg
     2281 cggacgccgg tacccgctgg tgggcacggt gagcatggac aacgtgaccg tggacctggg
     2341 cccggagacc gacgtcgagg tgggcgcggc ggccgtgctg atcggctccc agggcgacga
     2401 ggccgtgacc gccgaggaga tcgcgcggcg gctggggacc atcaactacg aggtcacctg
     2461 cggcctctcc gcccgcgtgc cgcgcctgga ggtctgacgg tgccggccac cgcgctggag
     2521 acggccgagc cggtcaaggc ggtgcgccag gccctggccg ggggcccgga cgccaggctg
     2581 gtcggcggcg tggtccgcga cgcccacctg ggccgcccgc tgggcgacct ggacctggcc
     2641 gtggacggcg accccgcaca ggtggcgcgg acggtggcga aggcgctcgg cggcccggtg
     2701 ttcccgctgt ctgaggcgtt cggctcctgg cgggcgctgg acggcgaccg gcgcttcgag
     2761 gtcgacgtg
//
LOCUS       DASUPW010000462         2157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1077004, whole genome shotgun sequence.
ACCESSION   DASUPW010000462 DASUPW010000000
VERSION     DASUPW010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077004"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VFY44_10370"
     CDS             <1..662
                     /locus_tag="VFY44_10370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5910890.1"
                     /translation="RRAANAAGAVVCVSEATAAEVVGVLGVDRACVVVAPHGPGQVEP
                     APEPRADRGDGPLLFVGDAEPRKNLAGLLAAYARYRAAAEDPAPLVLAGAAAAAAGEP
                     GVTGAPDPSPAELSALLDGARALVHPSLHEGFGLPLVEAMALGVPVVAVASAGAREVC
                     GQAALLVEPDGLAEAIGRVAGDAGLRTDLAGRGWERAGRYSWRASARAHLRAYTLALR
                     S"
     gene            659..1858
                     /locus_tag="VFY44_10375"
     CDS             659..1858
                     /locus_tag="VFY44_10375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5910891.1"
                     /translation="MNVAILGTRGIPASYSGFETAVEQITSRLTARGHTVTVYCRPHV
                     VDPGIREWKGAKLVHLPTVRNKYLDTFVHTLLSSLHASRRVKPDVALFFIAGNSPLCL
                     VTRAAGIPTVINVDGLDSDRRKWNRVAKSYLRAAERLSPRLATRAITDSHAVARIYES
                     RYGRGIGVVPYGAELPEDGGNDVLERLGLEPRRYVLFVGRLEPENNPHMLVEAWSRIP
                     SAKTKGMKLVVVGGAPYASEYINRVRRAADPRVVFPGYVFGSGYWQLQRNAYLFCAPT
                     EVGGTHPVILESLVAGNCVLVNDYEPNAETVGDAGVYFSGREGVPDLTRQLERLLDQP
                     DVVDGYRGKARERAALYSWDAVASAYEQLLLDVSESSGYGPLPMERLGELERATDPAG
                     GSSRAAV"
     gene            complement(1855..>2157)
                     /locus_tag="VFY44_10380"
     CDS             complement(1855..>2157)
                     /locus_tag="VFY44_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910892.1"
                     /translation="ALSEDGRLVDAYRVLEQAVTEHPRNPDTWIRLGQFELDDLGLPR
                     RALASSQAAATIDPASTRLRDLVEQIDAEVERQNRDVAAARALLDAQIRRQQGQQP"
BASE COUNT          258 a          791 c          825 g          283 t
ORIGIN      
        1 atcgccgcgc cgccaacgcc gcgggcgcgg tggtgtgcgt gtccgaggcc accgccgccg
       61 aggtggtcgg ggtgctgggg gtcgaccgcg cgtgcgtggt cgtcgccccg cacgggccgg
      121 gccaggtcga gccggcgccc gagccccgcg ccgaccgcgg cgacggtccg ctgctgttcg
      181 tgggcgacgc cgagccgcgc aagaacctgg ccgggcttct ggccgcctac gcccgctacc
      241 gggcggcggc cgaggacccc gccccgctgg tgctggccgg ggcggccgcc gcggcggcgg
      301 gcgagccggg cgtgaccggc gcgcccgacc ccagcccggc cgagctgtcc gcgctgctgg
      361 acggcgcgcg cgcgctggtg cacccgtcgc tgcacgaggg cttcggcctg ccgctggtgg
      421 aggccatggc cctgggggtg ccggtggtgg cggtggccag cgccggcgca cgcgaggtgt
      481 gcggccaggc ggcgctgctg gtggagcccg acgggctggc cgaggcgatc gggcgcgtgg
      541 cgggcgacgc cggcctgcga accgacctgg ccgggcgcgg gtgggagcgc gccgggcgct
      601 actcgtggag ggccagcgcc cgcgcccacc tacgcgccta taccctggcg cttcggtcat
      661 gaacgtcgcc atcctgggca cccgcggcat cccggcctcc tacagcggct tcgagaccgc
      721 cgtggagcag atcaccagcc ggctgaccgc gcgcgggcac acggtcaccg tgtactgccg
      781 cccgcacgtc gtggaccccg gcatccgcga gtggaagggc gcgaagctgg tgcacctgcc
      841 gacggtgcga aacaagtacc tggacacgtt cgtgcacacg ctcctgtcca gcctgcacgc
      901 gtcgcgccgg gtcaagcccg acgttgcgct gttcttcatc gcgggcaaca gcccgctgtg
      961 cctggtcacc cgcgccgccg gcatccccac cgtgatcaac gtggacggcc tggactcgga
     1021 ccggcgcaag tggaaccgcg tggccaagtc ctacctgcgc gccgccgagc ggctgtcccc
     1081 gcggctggcc accagggcca tcaccgactc gcacgcggtc gcgcgcatct acgagtcgcg
     1141 ctacgggcgc ggcatcgggg tcgtgcccta cggcgccgag ctccccgagg acggcggcaa
     1201 cgacgtgctg gagcgcctgg gcctggagcc gcgccgctac gtcctgttcg tcggccgcct
     1261 ggagcccgag aacaacccgc acatgctggt cgaggcgtgg tcgcggattc cgtcggccaa
     1321 gaccaagggc atgaagctcg tggtggtggg cggggcgccg tacgcgtccg agtacatcaa
     1381 ccgcgtacgc cgcgcggccg acccgcgcgt ggtgttcccc ggctacgtgt tcggcagcgg
     1441 ctactggcag ctccagcgca acgcctacct gttctgcgcc cccaccgagg tgggcggcac
     1501 gcatccggtg atcctggaat cgctggtggc gggcaactgc gtcctggtca acgactacga
     1561 gcccaacgcc gagaccgtgg gcgacgccgg cgtctacttc tccggccgtg agggcgtgcc
     1621 ggacctgacc cgccagctgg agcggctgct cgatcagccc gacgtggtcg acggctaccg
     1681 gggcaaggcc cgggagcggg ccgcgctgta ctcgtgggac gcggtggcgt ccgcgtacga
     1741 gcagctgctg ctggacgtgt cggagagcag cggctacggg ccgctgccca tggagcgcct
     1801 cggcgagctg gagcgcgcca ccgatccggc cggcggctcc tcgcgcgcgg ccgtctaggg
     1861 ctgctggccc tgctgacgcc ggatctgcgc gtccaggagc gcccgcgcgg cggccacgtc
     1921 ccggttctga cgctcgacct ccgcgtcgat ctgctccacc aggtcgcgca ggcgggtgga
     1981 ggcggggtcg atcgtggccg cggcctgtga cgaggccagc gcccggcggg gcaggcccag
     2041 gtcgtcgagc tcgaactggc ccaggcggat ccacgtgtcc gggttgcggg gatgctcggt
     2101 cacggcctgc tcgagcacgc ggtaggcgtc gaccagccgg ccgtcctcgg acagggc
//
LOCUS       DASUPW010000463         2409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_800106, whole genome shotgun sequence.
ACCESSION   DASUPW010000463 DASUPW010000000
VERSION     DASUPW010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800106"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="VFY44_10385"
     CDS             <1..993
                     /locus_tag="VFY44_10385"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5910893.1"
                     /translation="DELCGLEVTVPGRADGGPRLCLNGHLDVVAPGTERWEHGPWSGL
                     VAEGQVHGRGSVDMKGGVVAALHAMADRGPAAGCQVVLWAVASEEDGGQGTFAALEAD
                     RAFDACLIPEPTGLEVVCAQAGSLTFEGVVGGRAAHAGMRLEGESAIDNYVQVHMALA
                     AHERDINAGVEYDLMAALELPYPVSVGRVSGGEWASSVPDRVEFTGRLGVPVGQDLDE
                     ARAALRRALAGLPVQVSFTGGVFAPGATDPRHPFAALVLESFPGSSAVGVPWGADMRQ
                     YTALGIPAVMVGPGNAHLAHAVDERVPIDDLAALAEGIGRVIDGFGGPAHTLSP"
     gene            990..>2409
                     /locus_tag="VFY44_10390"
     CDS             990..>2409
                     /locus_tag="VFY44_10390"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyltransferase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HEX5910894.1"
                     /translation="MSEPRIGILIVAYNAASTLVKVLDRIPADFRERVTEVLVSDDYS
                     SDDTYLIGLGYQQDRTDLPITVIRQPHNLGYGGNQKAGYRWAIEQGLDIVVLLHGDGQ
                     YAPESLPDLVGPLVEGRCDAVLGSRMMVPGEARRGGMPLYKYVGNRILSTVENAVVGA
                     DLTEWHSGYRAYSVDALREIPFERNDDGFNFDTQVIVQMFEAGRRVEEVPIPTYYGDE
                     VCRVNGIGYARDVTRDVLRYRAHKMGFGTGETAFASQAYELKQADDSSHHRLATWLAA
                     RPPGRVLDLGCADGSLGERLGRAGHEVTGVDVVAGEHTRERLHAFVAGDLEQGIPDEV
                     GDGFDVVLCADVLEHVSAPGRLLSAARDRLAPGGVVVVSVPNFAHWYPRARVALGRFD
                     YDRRGILDSTHLRFFTRRSFERLAGQVGLEVSRREVTGLPLEVLDRSASEDTDGVAVG
                     SVLGRAVRAVDGAGQALRPGLFG"
BASE COUNT          299 a          836 c          948 g          326 t
ORIGIN      
        1 gacgagctct gcggcctcga ggtcacggtg cccggccgtg cggacggcgg tccgcggctg
       61 tgcctgaacg gccatctgga cgtcgtggcg ccgggcaccg agcggtggga gcacggcccc
      121 tggtcgggcc tggtcgccga aggccaggtg cacggccgcg ggtcggtgga catgaagggc
      181 ggggtggtgg ccgcgctgca cgcgatggcc gaccgcggtc ccgcggccgg atgccaggtg
      241 gtgctgtggg cggtggcctc ggaggaggac gggggccagg gcacgttcgc ggcgctggag
      301 gccgaccgcg cgttcgatgc ctgcctgatc ccggagccga ccggcctgga ggtggtgtgc
      361 gcgcaggcgg gatcgctgac cttcgagggg gtcgtcggcg gccgcgcggc ccacgccggc
      421 atgcgcctcg agggcgagtc cgccatcgac aactacgtgc aggtgcacat ggcgctcgcg
      481 gcccacgaga gggacatcaa cgccggtgtc gagtacgacc tgatggcggc cctggagctg
      541 ccctaccccg tgtcggtggg gcgcgtcagc gggggcgagt gggccagctc ggtgcccgac
      601 cgggtcgagt tcaccggccg ccttggcgtg ccggtgggcc aggacctgga cgaggcacgc
      661 gccgcgctgc gccgggcgct ggccggcctg ccggtgcagg tcagcttcac cggcggggtg
      721 ttcgcccccg gcgccaccga cccgcgccat ccgttcgcgg ccctggtgct ggagtcgttc
      781 ccgggctcga gcgcggtggg ggtgccctgg ggcgccgaca tgcgccagta caccgcgctg
      841 ggcatcccgg ccgtgatggt cgggccgggc aacgcccacc tggcgcacgc cgtcgacgag
      901 cgggtgccga tcgacgatct ggcggcgctg gccgagggca tcggccgcgt gatcgacggc
      961 ttcggcgggc cagcgcatac gctttcgccg tgagcgagcc gcgcataggc atcctgatcg
     1021 tggcgtacaa cgccgcgtcc acgctggtca aggtgctgga ccgcatcccc gcggacttcc
     1081 gcgagcgcgt gaccgaggtg ctggtgtccg acgactactc ctcggacgac acctacctga
     1141 tcgggctggg ctaccagcag gaccgcaccg acctgccgat cacggtcatc cgccagcccc
     1201 acaacctggg ctacggcggc aaccagaagg ccggctaccg ctgggcgatc gagcagggcc
     1261 tggacatcgt ggtgctgctc cacggcgacg gccagtacgc gccggagtcg ctccccgatc
     1321 tggtcggccc gctggtcgag gggcgctgcg acgcggtgct gggctcgcgc atgatggtcc
     1381 ccggcgaggc ccgccggggc ggcatgccgc tgtacaagta cgtgggcaac cggatcctgt
     1441 cgaccgtcga gaacgcggtg gtgggcgccg acctgacgga gtggcacagc ggctaccgcg
     1501 cgtacagcgt cgacgccctg cgcgagatcc cgttcgagcg caacgacgac ggcttcaact
     1561 tcgacaccca ggtgatagtg cagatgttcg aggcgggccg gcgggtggag gaggtgccga
     1621 tccccaccta ctacggcgac gaggtctgcc gggtgaacgg catcggctac gcccgcgacg
     1681 tgacccgcga cgtgctgcgc taccgcgccc acaagatggg gttcgggacg ggcgagacgg
     1741 cgttcgcctc ccaggcctac gagctcaagc aggccgacga cagctcccac caccgtctgg
     1801 ccacgtggct ggccgcccgc ccgcccgggc gggtgctgga cctgggctgc gccgacggga
     1861 gcctcggcga gcgcctcggg cgcgcggggc acgaggtcac cggcgtggac gtggtggcgg
     1921 gcgagcacac gcgcgagcgg ctgcacgcgt tcgtggcggg cgacctggag cagggcatcc
     1981 ccgacgaggt cggggacggc ttcgacgttg tgctctgcgc cgacgtcctg gagcacgtgt
     2041 cggcgccggg acggctgctg tccgccgcgc gcgaccggct ggcgcccggc ggcgtcgtgg
     2101 tggtcagcgt gcccaacttc gcccactggt acccgcgggc gcgggtggcg ctgggccggt
     2161 tcgactacga ccgccgcggc atcctggact ccacccacct gcgcttcttc acccgccgca
     2221 gcttcgagcg cctggccggc caggtggggc tcgaggtcag ccgccgcgag gtcacgggcc
     2281 tgccgctgga ggtgctggac cgcagcgcgt ccgaggacac ggacggcgtc gcggtgggct
     2341 cggtcctggg ccgggccgtg cgggccgtcg acggcgccgg gcaggcgctg cggcccgggc
     2401 tgttcggct
//
LOCUS       DASUPW010000464         3320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_107482, whole genome shotgun sequence.
ACCESSION   DASUPW010000464 DASUPW010000000
VERSION     DASUPW010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107482"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="VFY44_10395"
     CDS             <1..1064
                     /locus_tag="VFY44_10395"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEX5910895.1"
                     /translation="LSESGDVWQAQDTSPEEVAAALRELLKQRHAETEAFVPARVLNL
                     VVIVDRDWRGEIQNRLDKVGSYHASRTILVSVEPGRTTIDASAAMTAEEEVKRGELAV
                     AHEHVVLDIGEKHLKALDTIVDPLVVSDLATLVWSPHRHHEGVDAMAKLAQVILIDSQ
                     NTEGPEYAVKRSSELAQRHYVVDLAWLRSTPWRERVASTFDPAQWRRDLGSVSSVTVR
                     HKPDSAMAGYLLLGWLSSRLGWEPGAMTEHAGGAQGKAKAHKHEIKLALEPDETMTTP
                     GLAGITVESARGMSISLDRGPGGLTAHRQTPDGQDQTWTVTGASRGESGILGEGIRQA
                     LLRDHTYGPALAAAEQMLG"
     gene            1074..1361
                     /locus_tag="VFY44_10400"
     CDS             1074..1361
                     /locus_tag="VFY44_10400"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HEX5910896.1"
                     /translation="MPRFLVERDFGQVSDEQMQEFVATTKTLEADERFVELRWEGSRV
                     CDAEGAGIKSFCVYTAPSAQLLDEHTQALGQHGTHRIFEIVGDIDPRDIAV"
     gene            1372..1656
                     /locus_tag="VFY44_10405"
     CDS             1372..1656
                     /locus_tag="VFY44_10405"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HEX5910897.1"
                     /translation="MPRYVIERDFGQVSEDDLQEVAARSKMTAIDHFPDIRWEHSHVC
                     AGDDGAVKSFCVYEAPGADRLREHADQVGGHEVISVYEIVGDLTPEEVRV"
     gene            complement(1653..>3320)
                     /locus_tag="VFY44_10410"
     CDS             complement(1653..>3320)
                     /locus_tag="VFY44_10410"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5910898.1"
                     /translation="GDRSGALVAYSRFRERLRAELGISASADTRALVDGIREGSVDPA
                     PAGEDVGTPPRPAGPRAAGEWVPGTPFPLPPRLRQSAGGAGFVGREAELDTLWRLWAQ
                     VGEGGGARLALVAGQAGMGKTRLSRELALKAHQEGAIVLHGTANEDLLMPHQHVVEAL
                     AHLLAVAAPAELGRLVEPRAADLEPVAPNLQGAGAGDDAAKAPGESKRYRLFEAVAGL
                     LEELAGDAPVLLVIDDLHWADQSTAALLRHVLESRPGARLLVLATQRPGEGASEAQAE
                     ALQRLSQGQFVERVPLGGLSPEDIVRLARDVTERDLAPELARAIQAETHGNPFFAQEI
                     VRHLDESDRGASVLSLARGDVPERVREVVNLRLVRLSDACLRLLTVAAVMGTDFEPAL
                     LEQVSDLQGEDLAIALDEARAADVLVEADSGDHELFSFSHALVRRTLLARVTRAHKRR
                     IHARVAEALEASRGDGALLEIAHHLCEARPVSDRETALDYATRAAERATSGLAYAEAV
                     DLFTRARLLLPPDDPRRRKLALKRAIAYQALFHAVYDTSPSATGAPG"
BASE COUNT          471 a         1227 c         1212 g          410 t
ORIGIN      
        1 atttgagcga gtcgggcgac gtctggcagg cgcaggacac ctcgccggag gaggtcgctg
       61 ccgcgctgcg cgagctgctc aagcagcgtc acgccgagac cgaggcgttc gtgcccgccc
      121 gcgtgctgaa cctggtggtg atcgtcgacc gcgactggcg cggcgagatc cagaaccggc
      181 tggacaaggt gggctcctac cacgcctcgc gcacgatcct ggtcagcgtc gagcccggcc
      241 gcaccaccat cgacgcctcc gccgccatga ccgccgagga agaggtcaag cgcggcgagc
      301 tggccgtggc gcacgagcac gtcgtcctgg acatcgggga gaagcacctg aaggcgctgg
      361 acacgatcgt cgacccgctc gtggtcagcg acctggccac gctggtgtgg tcgcctcacc
      421 gccaccacga gggcgtggac gccatggcca agctggccca ggtgatcctg atcgactcgc
      481 agaacaccga gggccccgag tacgcggtca agcgatccag cgagctcgcc cagcgtcact
      541 acgtggtcga cctggcctgg ctgcgctcca ccccctggcg cgagcgcgtg gcctccacct
      601 tcgaccccgc ccagtggcgg cgcgacctgg ggtcggtctc ttcggtgacc gtgcgccaca
      661 agccggactc ggccatggcc ggctacctgc tgctgggctg gctgtcgtcg cggctggggt
      721 gggagcccgg cgccatgacc gagcacgccg ggggcgccca gggcaaggcc aaggcccaca
      781 agcacgagat caagctggcg ctggagcccg acgagaccat gaccacgccc gggctggcgg
      841 gcatcacggt cgagtccgca cgcgggatga gcatctcgct ggaccgcggc ccgggcgggc
      901 tgaccgccca ccggcagacc ccagacgggc aggaccagac gtggaccgtg accggcgcct
      961 cgcgcgggga gtccggcatc ctcggcgagg gcatccgcca ggcgctgctg cgcgaccaca
     1021 cctacggccc cgcgctggcg gcggcggagc agatgctggg ttaacgtagg cccatgccga
     1081 gattcctggt cgaacgggac ttcgggcagg tcagcgacga gcagatgcag gagttcgtcg
     1141 cgaccaccaa gaccctcgag gccgacgagc gcttcgtcga gctccggtgg gagggcagcc
     1201 gcgtctgcga cgccgaggga gcgggcatca agtccttctg cgtctacacc gcccccagcg
     1261 cgcagctgct ggacgagcac acgcaggcgc tgggccagca cggaacccac cgcatcttcg
     1321 agatcgtcgg tgacatcgat ccgcgcgaca tcgcggtgtg aggaggtcgg gatgcccagg
     1381 tacgtgatcg agcgcgactt cggacaggtc agcgaggacg acctccagga ggtggccgcg
     1441 cggtcgaaga tgacggccat cgaccacttt cccgacatcc gctgggagca cagccatgtc
     1501 tgcgccggcg acgacggcgc ggtcaagagc ttctgcgtct acgaggcgcc gggtgccgac
     1561 cgcctcaggg agcacgcgga ccaggtgggc ggccacgagg tcatcagcgt ctacgagatc
     1621 gtcggcgacc tgacccccga ggaggtcagg gtctagcccg gcgcgccggt cgcgctcggc
     1681 gacgtgtcgt acacggcgtg gaagagcgcc tggtaggcga tcgcgcgctt gagcgcgagc
     1741 ttgcgccggc gcgggtcgtc ggggggcagg agcagccggg cgcgcgtgaa caggtccacg
     1801 gcctcggcgt acgccaggcc cgacgtggcc cgctcggccg ccctggtggc gtagtccagc
     1861 gccgtctcgc ggtccgacac cggccgggcc tcgcacaggt ggtgcgcgat ctccagcagc
     1921 gcgccgtcgc cgcgcgaggc ctccagcgcc tctgccaccc gcgcatggat gcggcgcttg
     1981 tgcgcgcggg tcacgcgcgc gagcagggtg cggcgcacca gcgcgtggga gaacgagaac
     2041 agctcgtggt cgccgctgtc ggcctccacc agcacgtccg cggcccgcgc ctcgtcgagc
     2101 gctatggcca ggtcctcgcc ctggaggtcg ctgacctgct ccagcagggc cggctcgaag
     2161 tcggtgccca tcaccgccgc caccgtgagc agccgcaggc aggcgtcgga cagccggacc
     2221 aggcgcaggt tgaccacctc gcgcacccgc tcgggcacgt cgccgcgggc cagcgagagc
     2281 acgctcgcgc cgcggtctga ctcgtccagg tgccgcacga tctcctgcgc gaagaagggg
     2341 ttcccgtgcg tctcggcctg gatcgcccgg gccagctcgg gggcgagatc gcgctccgtg
     2401 acgtcgcggg cgagccggac gatgtcctcg ggggacagcc ccccgagggg cacgcgctcc
     2461 acgaactgtc cctgtgagag gcgctggagc gcctcggcct gggcctcgga tgccccttca
     2521 ccgggccgct gggtggccag cacgagcagc cgcgcgccgg ggcgggactc cagcacgtgg
     2581 cgcagcaacg cggccgtgga ctggtcggcc cagtgcaggt cgtcgatcac gagcaggacc
     2641 ggagcgtcac cggccagctc ctcgagcagg ccggccaccg cctcgaagag ccggtagcgc
     2701 ttgctctcgc ccggcgcctt cgccgcgtcg tctcctgccc cggccccctg gaggttgggc
     2761 gcgacgggct cgaggtcggc tgcccgcggc tcgaccaggc gacccagctc cgccggcgcg
     2821 gcgaccgcca gcaggtgggc gagcgcctcg accacgtgct ggtggggcat caggaggtcc
     2881 tcgttcgccg tgccgtggag cacgatcgcg ccctcctggt gcgccttgag cgccagctcc
     2941 cggctgaggc gcgtcttgcc catgcccgcc tggccggcca ccagcgccag gcgcgcgccg
     3001 ccgccctcgc cgacctgcgc ccacagccgc cacagcgtgt ccagctcggc ctcccggccc
     3061 acgaagccgg cgccccccgc gctctggcgc agccgcgggg gcagggggaa cggggtgccc
     3121 gggacccact cgcctgcggc ccgcggcccg gccggacgcg ggggcgtgcc cacgtcctcg
     3181 ccggccgggg cggggtcgac cgagccctcc cggatcccgt ccaccagcgc ccgggtgtcc
     3241 gcggaggccg agatgcccag ctctgcccgc agccgctcgc gaaagcgcga gtaggccacc
     3301 agcgccccgc tgcggtcgcc
//
LOCUS       DASUPW010000465         2623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_661120, whole genome shotgun sequence.
ACCESSION   DASUPW010000465 DASUPW010000000
VERSION     DASUPW010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661120"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VFY44_10415"
     CDS             complement(<1..604)
                     /locus_tag="VFY44_10415"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX5910899.1"
                     /translation="MEPAARAEQILVESGAILRGDHFVYVTGQHGDGWVAKDAVFPYT
                     DRVDELCGLLAGALADREYDYVCGPATGGLIVAQWVAHHLRLPAVFAEHAKEEGYVPE
                     HAGDGPLRAPFVLRRGYDEAVRGKRVLVVDDVVNTGLSVRETADAVRDAGGQVVTVAC
                     LCTRGNAEAGDVGSADFAWLTEVLVPSWPAADCPLCRDGVP"
     gene            668..1513
                     /locus_tag="VFY44_10420"
     CDS             668..1513
                     /locus_tag="VFY44_10420"
                     /inference="COORDINATES: protein motif:HMM:NF039362.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HEX5910900.1"
                     /translation="MNRIKRGWRLAKDSWAVLRADRSLTLFPVLSFVAAATAFALIMA
                     PGIVVAAAAEQEWVALPFFLVASYLATFATIYFNVALTGAAVKSMDGRDTNLHDGLAV
                     ARERRGLIAKWALVQWAVGLLIRVLQNAGDRGGVGQIVSALVSGLVGAAWAIASFFVV
                     PVLALEGVGPKDALKRSIAVIRERWGEGIVGHATIGGAVFLVGVVPALVVGGLGYVSF
                     ASVPALGGALIAVAVVALIAVGVIGSALNAIFRVTLYRYATEGRGSDAFADDDLAAAF
                     RPKKA"
     gene            complement(1515..>2623)
                     /locus_tag="VFY44_10425"
     CDS             complement(1515..>2623)
                     /locus_tag="VFY44_10425"
                     /inference="COORDINATES: protein motif:HMM:NF024732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5910901.1"
                     /translation="MRDRGYIDGKVPGLRHVSWFLSPHSRDAFHRGGCDLVPNSFSEV
                     PRLMRQVVKPALVVTAVSPPDRHGYFSLAANAEYAAALIGEVPFFVEVNPRLPRTFGG
                     NQLHASDLVGFCEADYAPAELPAHAMGDRDRAIAALVAERIPDGATLQAGIGAVPDCV
                     LGLLGEHKDLGVHTELLGDGMIDLVEGGVVTGTEKATHRNKIVTTTALGSARLYDFVA
                     DNPGVEFWPVEYTNDPGVIAREPHFTAINATVEVDFLGQCASEGLGSDYWSSSGGQPD
                     FARGAVTSEHGQAFIVVHSTTAQDTVSRIVPQLHPGAAVTTFKNIVDKVVTEYGVAEL
                     RGSSVRERTRRLIAIAHPDHRDQLTAKARELGYV"
BASE COUNT          337 a          961 c          944 g          381 t
ORIGIN      
        1 ccggaacgcc gtcgcggcac agggggcagt ccgcggccgg ccacgagggc accagcacct
       61 ccgtcagcca cgcgaagtcg gcgctgccca cgtcgccggc ctccgcgttg ccgcgggtgc
      121 acaggcacgc gaccgtgacc acctggccgc cggcgtcgcg gacggcgtcg gcggtctcgc
      181 gcaccgacag gccggtgttc accacgtcgt ccaccaccag cacgcgcttg ccccgtaccg
      241 cctcgtcgta gccgcggcgc aacacgaacg gcgcccgcag cgggccgtcg cccgcgtgct
      301 cgggcacgta gccctcctcc ttggcgtgct cggcgaacac ggccggcaga cggaggtggt
      361 gcgccaccca ctgggccacg atcagcccgc ccgtggccgg cccgcacacg tagtcgtact
      421 cgcggtccgc gagcgctccg gccagcagcc cgcacagctc gtcgacgcga tcggtgtacg
      481 ggaacaccgc gtccttcgcc acccagccgt cgccgtgctg gccggtcacg tagacgaagt
      541 ggtcgccgcg caggatcgcc ccggactcca ccaagatctg ctcggcgcgc gcggcgggct
      601 ccatgacgac gcgagtctaa ggacggggcg cgttcaagga cggccccgca tggccgacaa
      661 tggcggtatg aaccgaatca agcgcggatg gcgcctggcc aaggacagct gggcggtcct
      721 cagggccgac aggtcgctga ccctcttccc cgtcctgtcg ttcgtggccg ccgcgaccgc
      781 cttcgcgctg atcatggccc cgggcatcgt cgtcgctgcc gccgccgagc aggagtgggt
      841 cgcgctgccc ttcttcctcg tggcgtcgta cctggcgacc ttcgcgacga tctacttcaa
      901 cgtcgccctg acgggcgccg ccgtgaagtc catggacggc cgcgacacca acctgcacga
      961 cgggctggcc gtggcccgtg agcggcgcgg cctgatcgcg aagtgggcgc tcgtgcagtg
     1021 ggccgtcggg ctgctgatcc gggtcctgca gaacgccggc gaccgcggcg gagtgggtca
     1081 gatcgtcagc gccctggtct cggggctggt cggtgccgcc tgggccatcg ccagcttctt
     1141 cgtggtgccc gtgctggccc tggagggggt cgggcccaag gacgcgctca agcgctcgat
     1201 cgccgtcatc cgcgagcgct ggggcgaggg catcgtcggc cacgccacca tcggcggcgc
     1261 cgtgttcctg gtgggcgtcg tgccggcgct ggtcgtcggc gggctcggct acgtcagctt
     1321 cgcctcggtg ccggcgctgg ggggcgcgct gatcgccgtc gccgtggtcg cgctgatcgc
     1381 ggtcggggtg atcggctccg cgctgaacgc catcttccgg gtgaccctct accgctacgc
     1441 caccgaaggc cgggggtcgg acgcgttcgc ggacgacgac ctggccgccg ccttccgccc
     1501 caagaaggcc tgactcagac gtagcccagc tcgcgggcct tcgccgtcag ctggtcccgg
     1561 tggtccgggt gggcgatggc gatcagccgg cgggtgcgct cgcgcacgct gctgccgcgc
     1621 agctcggcca ccccgtactc ggtgaccacc ttgtcgacga tgttcttgaa cgtggtcacc
     1681 gcggcgccgg gatgcagctg gggcacgatc cgcgagaccg tgtcctgcgc ggtcgtggag
     1741 tgcaccacga tgaacgcctg cccgtgctcg gacgtcacgg cgccgcgggc gaagtcgggc
     1801 tggccgccgg acgaggacca gtagtcgctg cccaacccct cggacgcgca ctggcccagg
     1861 aagtcgacct cgaccgtcgc gttgatggcc gtgaagtgcg gctcgcgtgc gatcaccccg
     1921 gggtcgttgg tgtactccac cggccagaac tccacgcccg ggttgtcggc gacgaagtcg
     1981 tacagccggg cgctgcccag ggccgtggtg gtcacgatct tgttccggtg cgtggccttc
     2041 tcggtgccgg tgaccacgcc gccctccacc aggtcgatca tgccgtcgcc cagcagctcg
     2101 gtgtgcacgc cgaggtcctt gtgctccccc agcaggccga gcacgcagtc gggcaccgcg
     2161 ccgatgccgg cctggagcgt ggcgccgtcg gggatccgct cggccaccag cgcggcgatc
     2221 gcccggtcgc ggtcgcccat cgcgtgcgcg ggcagctcgg ccggtgcgta gtcggcctcg
     2281 cagaagccca ccaggtccga ggcgtgcagc tggttgccgc cgaacgtgcg cggcagccgc
     2341 gggttgacct ccacgaagaa gggcacctcg ccgatcagcg ccgccgcgta ctcggcgttc
     2401 gcggccagcg agaagtagcc gtggcggtcg ggcggcgaga ccgcggtgac gaccagcgcc
     2461 ggcttcacga cctggcgcat caggcggggc acctcgctga acgagttggg caccaggtcg
     2521 cagccgccgc ggtggaaggc gtcgcggctg tgtggcgaca ggaaccacga cacgtgccgc
     2581 agccccggca ccttcccgtc gatgtagccg cggtcgcgca tgg
//
LOCUS       DASUPW010000466         3316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_386161, whole genome shotgun sequence.
ACCESSION   DASUPW010000466 DASUPW010000000
VERSION     DASUPW010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386161"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VFY44_10430"
     CDS             complement(<1..609)
                     /locus_tag="VFY44_10430"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX5910902.1"
                     /translation="MTVLRETPVRPAARDAEVAADVRDIVEAVLRGDGLQVIRRLDGL
                     TVDSPDALRVPAGEIEAALAALDPDVRAGLELAAANVGALVEAQLRDPVDVTLGQGQQ
                     IRVDELPVARAGVYAPGGRAAYPSTVVMCAVTARAAGVERIVVCTPPGEDGHAHGVVL
                     AACALCGVDEVYRVGGVQAVAAMAYGLGPLEPVDVIAGPGNAY"
     gene            complement(606..1244)
                     /gene="hisG"
                     /locus_tag="VFY44_10435"
     CDS             complement(606..1244)
                     /gene="hisG"
                     /locus_tag="VFY44_10435"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX5910903.1"
                     /translation="MSRPALTIAVPRGALMGGTLDLLDTLGIDTAEVRANDRKLLFEE
                     AGIVTMRPSDVPTYVAQGAADIGVTGKDVLLEQPDRVVYELLDLGFGRCDMVVAARRG
                     DDAMDEALRRLGRVRVATKYPKITSAHFAETGRQVEIVEVKGSVELAPLTGMVDGIVD
                     LTATGTTLRENDLEVLEEITVCTARLIANPVAHKLKAEAIDELVARIRDGRS"
     gene            complement(1241..2200)
                     /gene="hisZ"
                     /locus_tag="VFY44_10440"
     CDS             complement(1241..2200)
                     /gene="hisZ"
                     /locus_tag="VFY44_10440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX5910904.1"
                     /translation="MIHPIPPGTRDVLPDEMREQRAITDGLHRTFEAAGYGEVWTPTL
                     EYEEVLGTGAGASGYRLFDDQGDVLALRSDMTLPIARLAATRFPDAEPPLRLCYFGHA
                     YRAARRGSGQATEFLQAGVELLGVPGPDGEAEVVDVLLQSLEAAGLARHRVGLGDSAL
                     YRELLANLEVPPEHHADLLARLAGKDLVGLEMRVEQLGLSDAARRLLSRLPEMRGGPE
                     MLERAHDVAGPALDGLARLYATLEERGLADRVILDLGLVRHMTYYTGAVYEVYDPAVG
                     FTLGGGGRYDDLVGRFGEPRAACGFALDVQRVHVARAAEEGLR"
     gene            complement(2219..>3316)
                     /locus_tag="VFY44_10445"
     CDS             complement(2219..>3316)
                     /locus_tag="VFY44_10445"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX5910905.1"
                     /translation="NAAAATTAELDTGLTERIRASFLLAGPLLARFGSALMPPPGGDV
                     IGRRRLDPHLDAFRALGATVEGRKSYAITAPRGGLRACDFFMDEPSVMATENALMAAA
                     LTPGSTVIHNAASEPHVQDLARLLLQMGARIEGIGSNVLVVHGEASLGGADYAIGPDY
                     IEVGSFIALAACTGGELRIKDCVADDLRMTRLAFERLGCKIEYDGADVIVPPGQALRI
                     KDDEGDAVSKIEDGPWPAFPADLTSIALALATQSEGMVLIHEKMFENRLFFVDKLVSM
                     GARVLLCDPHRAVVSGPSRLHGERMESPDIRAGMAMLIAALCAEGRSEIGNIRQIDRG
                     YERIDERLRGLGAHIERVPAEGARAPEQLPA"
BASE COUNT          425 a         1259 c         1206 g          426 t
ORIGIN      
        1 gtaggcgttg cccgggcccg cgatcacgtc cacgggctcc agcggcccga gcccgtaggc
       61 catggcggcc acggcctgga cgccgcccac ccggtagacc tcgtcgacgc cgcacagcgc
      121 gcaggcggcc aggaccacac cgtgggcgtg gccgtcctcg ccgggcggcg tgcacaccac
      181 gatccgctcc acgcccgccg cgcgcgcggt cacggcgcac atgaccaccg tggacgggta
      241 cgcggcgcgg cctccgggcg cgtacacgcc cgcgcgggcc acgggaagct cgtcgacacg
      301 gatctgctgg ccctgcccca gggtcacgtc gaccgggtcg cgcagctggg cctcgaccag
      361 ggcgcccacg ttcgccgcgg ccagctccag gccggcgcgg acatcggggt ccagggccgc
      421 cagcgcggcc tcgatctcgc cggcgggcac acgcagcgcg tcgggtgagt ccaccgtcag
      481 gccgtcgagc cgccggatga cctggaggcc gtcgccgcgc agcaccgcct cgacgatgtc
      541 gcgcacgtcg gcggccacct cggcgtcgcg cgcggccggg cggaccgggg tctcgcggag
      601 cacggtcacg agcggccgtc ccggatccgg gccaccagct cgtcgatggc ctcggccttg
      661 agcttgtggg ccaccgggtt cgcgatcagc cgcgcggtgc agacggtgat ctcctccagc
      721 acctcgaggt cgttctcacg cagcgtggtg ccggtggcgg tcaggtccac gatgccgtcg
      781 accatgccgg tcagcggcgc cagctccacc gatcccttga cctcgacgat ctcgacctgg
      841 cggcccgtct cggcgaagtg cgcggaggtg atcttggggt acttggtggc cacgcgcacc
      901 cgcccgaggc ggcgcagcgc ctcgtccatg gcgtcgtcgc cccggcgggc ggccacgacc
      961 atgtcgcagc ggccgaagcc caggtccagc agctcgtaga ccacgcggtc gggctgctcg
     1021 agcagcacgt ccttgccggt cacgccgatg tcggcggctc cctgggccac gtaggtgggc
     1081 acgtcggagg ggcgcatcgt cacgatgccc gcctcctcga acaggagctt gcggtcgttg
     1141 gcgcgcacct cggcggtgtc gatgccgagg gtgtccagca ggtccagcgt gccgcccatc
     1201 agggcgccgc gcggcacggc gatcgtcagc gcgggccggc tcatcgcagc ccctcctcgg
     1261 ccgcgcgcgc cacgtgcacg cgctgcacgt ccagggcgaa gccgcaggcg gcgcggggct
     1321 cgccgaagcg gccgaccagg tcgtcgtagc gcccgccgcc gcccagcgtg aagcccacgg
     1381 ccgggtcgta gacctcgtag accgcgcccg tgtagtaggt catgtgccgc accagcccca
     1441 ggtccaggat cacccggtcg gccaggccgc gctcctcgag cgtggcgtac agccgcgcca
     1501 ggccgtccag cgccgggccg gccacgtcgt gggcgcgctc cagcatctcg gggccgccgc
     1561 gcatctccgg gagccgtgac agcagccgcc gcgcggcgtc cgacaggccg agctgctcga
     1621 cccgcatctc cagccccacc aggtccttgc ccgccagccg cgccagcagg tcggcgtggt
     1681 gctcgggcgg gacctcaagg ttcgccagca gctcgcggta gagcgcgctg tcgcccaggc
     1741 ccacgcggtg ccgggccagc ccggcggcct ccagcgactg cagcagcacg tcgaccacct
     1801 cggcctcgcc gtcggggccg ggcacgccca gcagctcgac gccggcctgc aggaactcgg
     1861 tcgcctggcc gctgccccgg cgcgcggcgc ggtaggcatg gccgaagtag cacagccgca
     1921 gcggcggctc ggcgtcgggg aagcgggtgg cggccaggcg cgcgatcggc agcgtcatgt
     1981 ccgaccgcag cgccagcacg tccccctggt cgtcgaacag gcggtagccc gacgcgcccg
     2041 cgccggtgcc cagcacctcc tcgtactcca gggtcggggt ccacacctcg ccgtagccgg
     2101 cggcctcgaa cgtcctgtgc aggccgtcgg tgatggcgcg ctgctcgcgc atctcgtccg
     2161 gcagcacgtc gcgcgtgccc ggcggaatcg gatggatcac ggggtgcctc ctcgggccct
     2221 aggccgggag ctgctcgggc gcgcgggcgc cctcggcggg cacgcgctcg atgtgcgcgc
     2281 ccagcccgcg cagccgctcg tcgatccgct cgtagccgcg gtcgatctgg cggatgttgc
     2341 cgatctccga ccgcccctcg gcgcacagcg ccgcgatgag catggccatg cctgcccgga
     2401 tgtccgggct ctccatgcgc tcaccgtgaa gccggctcgg gccgctcacg accgcgcggt
     2461 gcggatcgca gagcagtacc cgagcgccca tggacacgag cttgtcgacg aagaacagcc
     2521 ggttctcgaa catcttctcg tggatcagga ccatgccctc ggactgggtg gccagcgcca
     2581 gcgcgatgct ggtcaggtcg gccgggaagg cgggccaggg cccgtcctcg atcttggaca
     2641 cggcatcgcc ctcgtcgtcc ttgatccgca gcgcctgccc cgggggcacg atcacgtcgg
     2701 cgccgtcgta ctcgatcttg cagcccaggc gctcgaaggc cagccgcgtc atgcgcaggt
     2761 cgtcggccac gcagtccttg atccgcagct cgccgccggt gcaggccgcg agcgcgatga
     2821 agctgcccac ctcgatgtag tccgggccga tggcgtagtc cgcaccgccg agggatgcct
     2881 cgccgtgcac gaccagcacg ttcgagccga tgccctcgat ccgggcgccc atctgcagca
     2941 gcaggcgggc cagatcctgg acgtggggct cggaggccgc gttgtggatc accgtggacc
     3001 cgggggtcag ggcggcggcc atcagcgcgt tctcggtggc catgaccgac ggctcgtcca
     3061 tgaagaagtc gcaggcgcgc agcccgcccc ggggcgcggt gatggcgtag gacttgcggc
     3121 cttcgaccgt ggcacccagc gcgcggaagg cgtccaggtg cgggtccagc cgccggcgcc
     3181 cgatcacgtc gccgccgggg ggcggcatca gggcggagcc gaagcgggcc agcagtgggc
     3241 cggccagcag gaacgaggcg cggatgcgct cggtcagccc ggtgtccagc tccgccgtcg
     3301 tcgcggcggc cgcgtt
//
LOCUS       DASUPW010000467         2762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_2048325, whole genome shotgun sequence.
ACCESSION   DASUPW010000467 DASUPW010000000
VERSION     DASUPW010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2048325"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VFY44_10450"
     CDS             complement(<1..274)
                     /locus_tag="VFY44_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5910906.1"
                     /translation="MPGDGGERGRLGEFTIEAEIGRGGMGVVYLAHQPSLDRRVALKV
                     IVPELSEDPAFRDRFEREARHVASLDHPNIVPIFGAGEADGRLYIAM"
     gene            complement(277..600)
                     /locus_tag="VFY44_10455"
     CDS             complement(277..600)
                     /locus_tag="VFY44_10455"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5910907.1"
                     /translation="MADWRLLSNHGRVLVFIGREPKARLRDIADGVGLTERSVHRIVS
                     ELCDDGCLRRIPNGRTNHYEIQPETPVHDPLLGEHWVGEILAVVAGSAAFPRREADLP
                     AEADG"
     gene            complement(666..739)
                     /locus_tag="VFY44_10460"
     tRNA            complement(666..739)
                     /locus_tag="VFY44_10460"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(703..705),aa:Arg,seq:tct)
     gene            complement(746..1690)
                     /locus_tag="VFY44_10465"
     CDS             complement(746..1690)
                     /locus_tag="VFY44_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910908.1"
                     /translation="MRAAAFTLLIGLLLPAAAAGAATPAGVAGALERDPVYAEDGAKP
                     GLTAAEQGRLRIQIARQDIGRVKVAVVTQASADRAGGLAEFAKAVDRDLKAKGNLIVV
                     GGPGIHLITSYAPPEPATDALRKGVQENNDSSLADRLSAGIEGIAKVDPGASGDLGAG
                     RTQSAPPSQLPDLDEATDSVVDTVKLVLLIIAAAVALPFVIGAVWIVLRIRRRRRDEA
                     EVLAEERDAFRREVVSLGDQIRELDLDVSMPDADPEGRAAYERALAVYDEANAALTMR
                     RSPASLQRARAALQEGHQRMAEAKRLLEAQLAARNAPQ"
     gene            1740..2408
                     /locus_tag="VFY44_10470"
     CDS             1740..2408
                     /locus_tag="VFY44_10470"
                     /inference="COORDINATES: protein motif:HMM:NF022589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HEX5910909.1"
                     /translation="MGSLLAQVVPLAAGAAVSPGLLALQMLNLSRPSGGMARAWSVLA
                     GAAAVVLAVSVGALFVTVSLGDLGRDRDLRDVIRLVAAVALVGVAVFELLHPARPPRR
                     PADAPAPEDPGRGPVHMASVGLGAALVAPNMALYVPAAHEIARSTVSAPEKLLAFAVA
                     AAVALIPVAGPPVLVAVLGDRARPALEALNAFVTRNSRVVTIVACLVFAGYLAVTGIV
                     GLAG"
     gene            2421..>2762
                     /locus_tag="VFY44_10475"
     CDS             2421..>2762
                     /locus_tag="VFY44_10475"
                     /inference="COORDINATES: protein motif:HMM:NF022589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HEX5910910.1"
                     /translation="MGSLLSQVLPLAAGAAVSPAILTLQLLVLARGAGAVRRSWFIAA
                     GAASVLVVEAVLALSLAEGTGGPGTPPDWRSAVKLAAAVVLLVMGIRNLVRTPAPKKD
                     EPGGGIGPGRAF"
BASE COUNT          330 a         1027 c         1017 g          388 t
ORIGIN      
        1 gcatcgcgat gtaaaggcgg ccatccgcct cacccgcgcc gaagatggga acgatgttgg
       61 ggtggtcgag cgacgccaca tggcgcgcct cgcgctcgaa ccggtcgcgg aaggcggggt
      121 cctcggagag ctccggaacg atcaccttca gcgcaacccg ccgatccagg ctcggctggt
      181 gagcgaggta caccacgccc atgcccccgc ggccgatctc ggcctcgatg gtgaactcac
      241 ccagccggcc ccgctccccc ccgtctcccg gcatgctcag ccgtcagcct ccgccgggag
      301 gtccgcttcg cgacgcggaa acgccgcgct tcccgccacg acggcgagaa tctcgccgac
      361 ccagtgttcg cccaggagcg ggtcgtggac cggcgtctcg ggctggatct cgtagtggtt
      421 cgtacggccg ttgggaatgc ggcgaagaca cccgtcgtcg cacagctcgc tgacgatgcg
      481 gtgcacgctg cgctcggtca gaccgacgcc atccgcgatg tcacgcaggc gcgccttcgg
      541 ctcccgcccg atgaacacca ggacccgccc gtgattcgac agcagtcgcc aatccgccac
      601 cccgccaggc taagccggta gcccgcccga cgcaagacac tcaacacatg aaaaacattg
      661 caacatgccc ccggctggac tcgaaccagc gaccctccgc ttagaaggcg gacgctctat
      721 ccagctgagc tacgggggcg cgtggtcatt gtggcgcgtt gcgcgccgcc aactgcgcct
      781 ccagcaggcg cttggcctcg gccatccgct ggtggccctc ctggagggcg gcgcgggcgc
      841 gttgcaacga cgccgggctg cgccgcatgg tcagcgccgc gttggcctcg tcgtagacgg
      901 ccagcgcgcg ctcgtaggcg gcccgcccct cggggtcggc gtcgggcatc gagacgtcca
      961 ggtcgagctc gcggatctgg tcgcccaggg acacgacctc cctgcggaag gcgtcgcgct
     1021 cctcggccag cacctcggcc tcgtcgcggc gccggcgacg gatccgcagc acgatccaga
     1081 cggccccgat cacgaacggc agcgccacgg cggcggcgat gatcagcagc acgagcttga
     1141 ccgtgtcgac caccgagtcg gtggcctcgt ccaggtcggg gagctgcgac gggggcgccg
     1201 actgggtgcg gccggccccg aggtccccgg acgcgcccgg gtcgaccttg gcgatgccct
     1261 cgatgcccgc cgagagccgg tcggccaggc tggagtcgtt gttctcctgc acgcccttgc
     1321 gcagcgcgtc ggtggccggt tccgggggcg cgtaggaggt gatcaggtgg atgcccggcc
     1381 cgccgaccac gatcaggttg cccttggcct tcaggtcgcg gtccaccgcc ttggcgaact
     1441 cggccagccc gccggcgcgg tcggcgctgg cctgggtgac cacggccacc ttcacccgcc
     1501 cgatgtcctg gcgcgcgatc tggatccgca gccggccctg ctcggccgcg gtcaggccgg
     1561 gcttcgctcc gtcctcggcg tagacggggt cccgctccag cgctcccgcc acgccggccg
     1621 gggtggcggc gccggccgcg gcggctggca gcagcaggcc gatcagcagg gtgaatgcgg
     1681 ccgcgcgcac gccgccagaa tcgcatacgg cggccgcgac ggccggtagc ctcgcgccgg
     1741 tgggctccct cctggcgcag gtcgtgccgc tggcggccgg agcggcggtc agcccggggc
     1801 tcctggcgct gcagatgctc aacctctccc gcccctcggg gggcatggcc cgggcctggt
     1861 cggtgctcgc cggcgccgcg gcggtggtcc tggcggtgtc cgtcggcgcc ctgttcgtga
     1921 ccgtgagcct gggcgacctg ggccgcgacc gggacctgcg cgacgtgatc cggctggtgg
     1981 ccgcggtggc gctcgtcggc gtggcggtgt tcgagctcct gcaccccgcc cggcccccgc
     2041 ggcgacccgc cgacgcaccg gcgcccgaag atcccgggcg cgggccggtg cacatggcct
     2101 ctgtcggcct gggggccgcg ctggtggccc cgaacatggc gctgtacgtg ccggccgcgc
     2161 acgagatcgc ccgctcgacc gtgtcggccc cggagaagct gctggccttc gcggtggcgg
     2221 ccgcggtggc gctcatcccg gtggcgggcc caccggtgct ggtggcggtc ctgggggacc
     2281 gcgcgcgccc cgcgctcgag gccctgaacg cgttcgtcac gcgcaacagc cgcgtggtca
     2341 cgatcgtcgc ctgcctggtg ttcgcggggt acctggcggt caccgggatc gtcggcctgg
     2401 cggggtaggg tcggccgccc gtgggctcgc tcctcagcca ggtcctgccg ctggccgccg
     2461 gcgccgccgt gagcccggcg atcctgacgc tccagctgct cgtcctggcc aggggagcag
     2521 gagcggtcag gcgatcgtgg ttcatcgccg ccggggccgc gtcggtgctg gtcgtcgagg
     2581 cggtgctggc gctgtcgctg gccgagggca ccggtgggcc ggggacgcca cccgactgga
     2641 gatcggcggt caagctggcc gccgccgtgg tcctgctcgt catgggcata cggaacctgg
     2701 tccgcacgcc ggcgccgaag aaggacgagc ccgggggcgg catcggcccg ggccgcgcgt
     2761 tc
//
LOCUS       DASUPW010000468         3172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1077657, whole genome shotgun sequence.
ACCESSION   DASUPW010000468 DASUPW010000000
VERSION     DASUPW010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077657"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..243)
                     /locus_tag="VFY44_10480"
     CDS             complement(1..243)
                     /locus_tag="VFY44_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910911.1"
                     /translation="MFDEDEEFTQGTGDGGADGPAEPGPEGPADGGADSGDGGADGPA
                     EPGPEGPADGGAGTGDGGADGPAEPGPEGPADGGAE"
     gene            complement(316..1212)
                     /locus_tag="VFY44_10485"
     CDS             complement(316..1212)
                     /locus_tag="VFY44_10485"
                     /inference="COORDINATES: protein motif:HMM:NF004846.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX5910912.1"
                     /translation="MSHKWTAADLPSQSDRTIVVTGANSGIGLVAARELAGAGARVVL
                     AVRDTSKGSTAAGSIAGDTEVQKLDLADLSSVHAFADGFDGRIDVLINNAGVMAVPES
                     RTKDGFEMQTGTNHLGHFALTNLLLPKITDRVVTVSSGAHRIGNLDLDDLNWEKRKYQ
                     SWKAYGQSKLSNLLFTMELEHRLAEAGSELHAYACHPGYASTNLQSHTGNVIQNTLMA
                     VGNKLLAQSDAMGALPTLYAATQDLPGGSYVGPDSFQEQRGHPTLVGRSGRASDPAVA
                     KRLWELSEEMTGVSFPLAGATA"
     gene            1291..2208
                     /locus_tag="VFY44_10490"
     CDS             1291..2208
                     /locus_tag="VFY44_10490"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5910913.1"
                     /translation="MLALAACGDSGGGGAEGEQATVKVQDTAGVPAAFLQYGVEKGYF
                     KKRKLDVKVQPSQGGATVIPSVVSGDTDIGGSNLVSVLLAQSKGVPVKIVAPGTFVRT
                     KPAQDFSGIVVAKDSDIRSPKDLEGKTLAVNTLKNVNDTTVSESLRKQGVDPARIKLL
                     EVDFPDMTAAVEGGRVDAAMEIEPFLSGAVKAGARIIDRPYTGTRPGLQIGSYFASEQ
                     YIAKNEDVVERFREGVAETAEAIESDQAAFREFLPTAAKIPPAAAQKAQLPTWKAESD
                     QSSIELTAQLMKRYGVTKEEPDAGEAQSK"
     gene            2205..>3172
                     /locus_tag="VFY44_10495"
     CDS             2205..>3172
                     /locus_tag="VFY44_10495"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5910914.1"
                     /translation="MKIDAFCHVLPRPYYDRLQDIQTAGAANLLKRTAPIASLYDMDE
                     RERIVAPFGDDYRQIISLAAPQIEALGDPSTTVDLARVANDAMAELVGPGEERFVGFV
                     AGLPLNDVDASLEEIDRAVNDLGALGIQIYTHVNGRPLDDPEFAPLFAKMAELDRPIW
                     VHPSRNSSWPDYPLAEDKSKFEIWWTLGWPYDTSVFMARLAFSGVFERHPDLRVITHH
                     AGGMIPFFSGRVGPGWDQFGARTPDDEAHLVDSKLTGRPVDTFHKFYADTAVFGAPHA
                     LQAAYEFFGADHILFASDMPFDPVQGQFIRTTIEDVDALRCTEQERA"
BASE COUNT          467 a         1127 c         1112 g          466 t
ORIGIN      
        1 ctactcggcg ccgccgtcgg cgggaccctc gggcccgggc tcggcggggc cgtccgcgcc
       61 gccgtcaccg gtgcccgcgc cgccgtcggc gggaccctcg ggtccgggct cggcggggcc
      121 gtccgcgccg ccgtcgccgg aatccgcgcc gccgtcggcg ggaccctcgg gtccgggctc
      181 ggcggggccg tccgcgccgc cgtcgccggt gccctgggtg aactcttcgt cttcgtcgaa
      241 catgtgacct ccgcgttgct gattgtcgct ctaggggttt cccggcaacg ctactgccat
      301 gcctcggcgc gcaacctagg ccgtcgcgcc cgccagcggg aagctcacgc cggtcatctc
      361 ctcggagagc tcccacaggc gcttggccac ggccgggtcg ctggcgcggc cgctgcggcc
      421 caccagggtc gggtggccac gctgctcctg gaaggagtcc gggccgacgt agctgccgcc
      481 cgggaggtcc tgggtggccg cgtacagggt gggcagcgcg cccatggcgt cgctctgggc
      541 caggagcttg ttgcccaccg ccatcagcgt gttctggatc acgttgccgg tgtggctctg
      601 gaggttggtc gacgcgtagc cggggtggca cgcgtaggcg tgcagctcgg acccggcctc
      661 ggccaggcgg tgctccagct ccatcgtgaa cagcaggttc gagagcttgg actggccgta
      721 ggccttccac gactggtact tgcgcttctc ccagttcagg tcgtccaggt ccaggttgcc
      781 gatccggtgc gcgcccgacg acacggtgac cacgcggtcc gtgatcttcg gcagcagcag
      841 gttggtcagc gcgaagtggc ccaggtggtt cgtgccggtc tgcatctcga agccgtcctt
      901 ggtgcgcgac tcgggcaccg ccatgacgcc ggcgttgttg atcagcacgt cgatccggcc
      961 gtcgaacccg tcggcgaagg cgtgcacgga ggacaggtcg gccaggtcga gcttctgcac
     1021 ctcggtgtcg ccggcgatcg agcccgcggc ggtgctgccc ttcgaggtgt cgcgcacggc
     1081 caggaccacg cgggcgcccg cgccggccag ttcacgcgcc gccaccaggc cgatgccgct
     1141 gttcgcgccg gtcacgacga tcgtccggtc gctctgggag ggaaggtcgg ctgctgtcca
     1201 cttgtggctc atggaatgag cctacgcacc ggcgggtacc tgccccctat gaggagagga
     1261 gccgcggcgg tcgctgccgc aatcatcgcc atgctcgcgc tcgccgcctg tggagacagc
     1321 gggggcgggg gagccgaggg agagcaggcc accgtgaagg tgcaggacac ggccggggtg
     1381 ccggccgcct tcctgcagta cggggtcgag aagggttact tcaagaagcg caagctcgac
     1441 gtgaaggtgc agcccagcca gggcggcgcc accgtgatcc cgtcggtggt ctccggcgac
     1501 accgacatcg gcggctcgaa cctggtctcc gtgctgctgg cccagagcaa gggcgtcccg
     1561 gtcaagatcg tcgcgcccgg caccttcgtc cggaccaagc cggcgcagga cttctcgggc
     1621 atcgtcgtcg cgaaggacag cgacatccgc tcgcccaagg acctggaggg caagacgctg
     1681 gcggtgaaca cgctcaagaa cgtgaacgac accacggtca gcgaatccct gcgcaagcag
     1741 ggcgtcgacc ccgccaggat caagctgctg gaggtcgact tccccgacat gaccgcagcc
     1801 gtcgagggcg ggcgcgtgga cgcggccatg gagatcgagc cgttcctgag cggcgccgtc
     1861 aaggcgggcg cccggatcat cgaccggccc tacaccggca ccaggcccgg gctgcagatc
     1921 gggtcgtact tcgccagcga gcagtacatc gccaagaacg aggacgtggt cgagcgcttc
     1981 agggagggcg tggccgagac cgcggaggcc atcgagtccg atcaggccgc cttccgcgag
     2041 ttcctgccga ccgcggccaa gatcccgccg gcggccgccc agaaggccca gctgccgacc
     2101 tggaaggccg agagcgacca gtcctcgatc gagctcacgg cccagttgat gaagcgctac
     2161 ggcgtgacca aggaagagcc cgacgccggg gaggcacagt ccaagtgaag atcgacgcgt
     2221 tctgccacgt cctgccgcgg ccctactacg accggttgca ggacatccag accgccggtg
     2281 cggccaacct gctcaagcgc acggcgccga tcgcctcgct ctacgacatg gacgagcgcg
     2341 agcggatcgt cgcgcccttc ggcgacgact accgccagat catcagcctg gccgcgccgc
     2401 agatcgaggc gctgggcgac ccgtcgacca cggtcgacct ggcccgggtg gccaacgacg
     2461 ccatggccga gctcgtgggc cccggcgagg agcggttcgt cggcttcgtg gcggggctgc
     2521 cgctgaacga cgtggacgcc tcgctggagg agatcgaccg ggccgtgaac gacctggggg
     2581 cactgggcat ccagatctac acgcacgtga acggccgccc gctggacgac ccggagttcg
     2641 cgcccctgtt cgccaagatg gcggagctgg accgcccgat ctgggtccac ccgtcgcgca
     2701 actcgtcctg gcccgactac ccgctggccg aggacaagtc caagttcgag atctggtgga
     2761 cgctgggctg gccgtacgac acctcggtgt tcatggcgcg gctggccttc tccggcgtct
     2821 tcgagcgcca ccccgacctg cgcgtgatca cccaccacgc cggcgggatg atcccgttct
     2881 tctccggccg cgtgggcccg ggctgggacc agttcggggc caggacgccc gacgacgagg
     2941 cccacctggt ggactccaag ctcaccggcc gcccggtcga caccttccac aagttctacg
     3001 ccgacaccgc cgtcttcggc gctccgcacg cgctccaggc ggcctacgag ttcttcggcg
     3061 ccgaccacat cctgttcgcc agcgacatgc ccttcgaccc cgtccagggc cagttcatcc
     3121 ggaccacgat cgaggacgtc gacgcgctcc ggtgcaccga gcaggagcgg gc
//
LOCUS       DASUPW010000469         4915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1909528, whole genome shotgun sequence.
ACCESSION   DASUPW010000469 DASUPW010000000
VERSION     DASUPW010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1909528"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="VFY44_10500"
     CDS             <1..1070
                     /locus_tag="VFY44_10500"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX5910915.1"
                     /translation="LLDGAVPVELLTGSTGAARRRDLLGRLESGQLNLVVGTHALLED
                     PVRFASLSLCVVDEQHRFGVRQRAALDAKAPDGLAPHALHMTATPIPRTLALTAYGDL
                     ETTVLRHLPAGGRPVQTHVVDGARARARAYERVREEIAKGRQCFVVCPLVSESEALQA
                     AAATDEAERLRATEFRDHAVGLIHGQMSSADKQEAMRGFADGETDVLVATSVIEVGID
                     VPNATVMLIEAAERYGLSQLHQLRGRVGRGRHESVCILFGDPKLPRLEAIASDKDGFA
                     LAEVDLDLRGAGEVLGTRQSGMPEFRVARLPEDQELLERARVHADATIAADPALEQPE
                     HALLRGAVIDRFGSDLDPIPA"
     gene            1067..1591
                     /gene="rsmD"
                     /locus_tag="VFY44_10505"
     CDS             1067..1591
                     /gene="rsmD"
                     /locus_tag="VFY44_10505"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX5910916.1"
                     /translation="MRVIAGTHRGRPLTRPPDARPTADRVREALFSMLGDVTDADVLD
                     LYAGSGALGIEALSRGAASAVFVDSSRQSTEAVQANLTALGERGEVVRRDALDYLRAA
                     PGTRRFDLVLCDPPYDSALAVADSLAELLPAVLYDTSVTVTESDTRTPLDLPFPVVRE
                     RAYGNTRIVIHRGF"
     gene            1581..2081
                     /gene="coaD"
                     /locus_tag="VFY44_10510"
     CDS             1581..2081
                     /gene="coaD"
                     /locus_tag="VFY44_10510"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX5910917.1"
                     /translation="MGSRNGGTAIVPGSYDPVTLGHLDIISRAAEVFDNVIVGVVNAS
                     VRKNKSVFSVEDRVAFIEGEITDLDNVEVKPFSNLLVEFARENGAKAVVKGLRAISDF
                     EYEFEMNQLNRKMAPDIESMYLMAAPEYSFLSSSGVKEIATFGGDLTGLVPAPVASRL
                     QEALKR"
     gene            2101..2565
                     /locus_tag="VFY44_10515"
     CDS             2101..2565
                     /locus_tag="VFY44_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910918.1"
                     /translation="MDVLVLIDKLDDTVHNAKPVPLTDQVRVDKEEIYDILDQMRATI
                     PEEIKQARWIVKERQEMLAEAKRESERIIREAREEQARLIGDEEVVKQAERQADEIVE
                     DARAREREIRLGAEDYADDILNTLEINLEKFTAAVQRGRDRLQRREEPADVA"
     gene            2586..3104
                     /locus_tag="VFY44_10520"
     CDS             2586..3104
                     /locus_tag="VFY44_10520"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX5910919.1"
                     /translation="MAPLTDTFDLGTLRLASGEGAHVDLEVAVEPLEFGGQTYTVPGD
                     RVAVRLDVSRPTGSGFALRLRFEAPLDGPCMRCLENADAVLAVDAREVDQPGGGDELS
                     SPYLGDEQELDVHGWARDALALALPTQIVCREDCAGLCPECGENLNHHPDHAHEPEPD
                     PRWAKLSELKLD"
     gene            3128..3325
                     /gene="rpmF"
                     /locus_tag="VFY44_10525"
     CDS             3128..3325
                     /gene="rpmF"
                     /locus_tag="VFY44_10525"
                     /inference="COORDINATES: protein motif:HMM:NF013908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX5910920.1"
                     /translation="MAVPKQRQSSARTNKRRSQHKIEAPSLAVCPRCGAPRIPHRVCG
                     ECGTYAGREIFRPAVDETDDE"
     gene            3336..4340
                     /locus_tag="VFY44_10530"
     CDS             3336..4340
                     /locus_tag="VFY44_10530"
                     /inference="COORDINATES: protein motif:HMM:NF014554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910921.1"
                     /translation="MIAVDANGADAGPAVVAEGARLSGEPTLVFGHGLPAGDGIVEAP
                     GVPASDNPVRAVRSNPDASIVQAAAAVADGRAEALVSAGSTGPTLAAATLAIKRLKGV
                     HRPALAVLVPIPAKPVLLLDCGANVEVRPEHLVQFAYMGSAFMEVVMGVERPSVGLLS
                     VGEESGKGTPDVLEARERLEGGPLNFTGNLEGFDLPGAGADVVVADGFTGNIALKVLE
                     GTSRVVRDAIRDAITSGPVSSAGGLLIRGAVGRLRDSIDPENVGGAILLGLRKPVVVG
                     HGSFGPRGIASAVALARRAVDEDMVGRTHAALEAAGALRSAPAGSFATVKPETPGTPG
                     "
     gene            4354..4608
                     /gene="acpP"
                     /locus_tag="VFY44_10535"
     CDS             4354..4608
                     /gene="acpP"
                     /locus_tag="VFY44_10535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX5910922.1"
                     /translation="MEREEVMRRIREHLAAELEVDASGITDATRFKEDLEADSLDLVE
                     LTVELEDTYGIRIPDEEAARILTVGQAADYVAAHADDAES"
     gene            4586..>4915
                     /locus_tag="VFY44_10540"
     CDS             4586..>4915
                     /locus_tag="VFY44_10540"
                     /inference="COORDINATES: protein motif:HMM:NF025973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HEX5910923.1"
                     /translation="MPTTPRAETGESLGDLLDQLPEDLARQAFTHASWVDHRAESYER
                     LAFLGDVVLSLAVSTHLYPRFERYGAGRLTKVRAQAVSGASCAAVALDLEVPRRLGER
                     APDGGAGP"
BASE COUNT          706 a         1791 c         1769 g          649 t
ORIGIN      
        1 ggctgctgga cggggcggtg ccggtggagc tgctgacggg atccaccggg gcggcgcgcc
       61 gtcgcgacct gctggggcgg ctggagtcgg gccagctgaa cctggtggtg ggcacccacg
      121 cgctgctcga ggacccggtc cggttcgcgt cgctgagcct gtgcgtggtc gacgagcagc
      181 accggttcgg cgtccgccag cgcgccgccc ttgacgccaa ggccccggac gggctggccc
      241 cgcacgccct gcacatgacg gcgacgccga tcccgcgcac gctggccctg accgcctacg
      301 gcgacctgga gaccacggtc ctgcgccacc tgcccgcggg cgggcggccc gtgcagaccc
      361 acgtcgtcga cggcgcccgc gcccgggcgc gggcctacga gcgggtgcgc gaggagatcg
      421 ccaagggccg ccagtgcttc gtggtctgcc cactggtgtc cgagtccgag gcgctccagg
      481 ccgcggcggc caccgatgag gccgagcggc tgcgggccac ggagttccgc gaccacgcgg
      541 tcgggctgat ccacggccag atgtccagcg ccgacaagca ggaggccatg cgcggcttcg
      601 ccgacggcga gaccgacgtc ctggtggcca ccagcgtgat cgaggtcggc atcgacgtgc
      661 ccaacgccac ggtcatgctc atcgaggcgg ccgagcgcta cgggctctcc cagctgcacc
      721 agctgcgcgg gcgcgtgggc cggggccggc acgagtcggt gtgcatcctg ttcggtgacc
      781 cgaagctgcc gcggctggag gcgatcgcct cggacaagga cggcttcgcg ctggcggagg
      841 tggacctgga cctgcgcggg gcgggggagg tgctggggac gcgccagagc ggcatgcccg
      901 agttccgggt ggcgcgactg cccgaggacc aggagctgct ggagcgggcg cgcgtacacg
      961 ccgacgccac gatcgccgcc gacccggcgc tcgagcagcc cgagcacgcg ctgttgcgcg
     1021 gcgccgtgat cgaccgcttc ggctccgacc tggacccgat cccggcgtga gggtcatcgc
     1081 gggcacgcac cggggccggc cgctgacgcg cccgccggac gcgcggccga cggccgaccg
     1141 cgtgcgcgag gcgctgttct cgatgctggg cgacgtgacc gacgcggacg tgctcgacct
     1201 gtacgcggga tcgggggcgc tgggcatcga ggcgctctcg cggggggcgg cctccgcggt
     1261 gttcgtcgac tcctcgcgcc agtcgaccga ggccgtccag gccaacctga ccgccctcgg
     1321 cgagcgcggc gaggtcgtgc gccgcgacgc cctggattac ctccgggccg cgccgggcac
     1381 ccgccgcttc gacctcgttc tttgcgaccc gccgtatgat tccgccctcg ccgttgcgga
     1441 ctcgctggcc gagttgcttc cggcggtcct ctacgacacc tccgtgaccg tgaccgagtc
     1501 agacacccgc acgccgctcg acctgccctt cccggtggtc cgcgagcgcg cgtacggaaa
     1561 caccaggatc gtgatccacc gtgggttcta ggaacggcgg gacggccatc gtgcccgggt
     1621 cctacgaccc ggtgaccctg ggccacctgg acatcatctc ccgtgccgcc gaggtgttcg
     1681 acaacgtgat cgtcggcgtc gtcaatgcgt ccgtgcgcaa gaacaagagc gtcttcagcg
     1741 tcgaggaccg ggtcgcgttc atcgagggcg agatcacgga tctggacaac gtcgaggtca
     1801 agccgttctc gaacctgctg gtggagttcg cgcgggagaa cggcgccaag gccgtggtca
     1861 agggactgcg cgccatctcc gacttcgagt acgagttcga gatgaaccag ctgaaccgca
     1921 agatggcccc ggacatcgag tcgatgtacc tgatggccgc gcccgagtac agtttcctct
     1981 cctcgagcgg cgtgaaggag attgcaacgt ttggtggcga cctcaccggg ttggtcccgg
     2041 ccccggtagc ctcccgtttg caggaagctt tgaagcgctg acgaactgag gcccgagcct
     2101 atggacgttc tcgtactcat cgacaagctg gacgacaccg tccacaacgc caagcccgtc
     2161 cccctgacgg accaggttcg cgtcgacaaa gaggagatct acgacatcct cgatcagatg
     2221 cgggccacga tccccgagga gatcaagcag gcccgctgga tcgtcaagga gcgccaggag
     2281 atgctggccg aggccaagcg cgagtccgag cggatcatcc gcgaggcgcg tgaggagcag
     2341 gcccgcctga tcggcgacga agaggtcgtc aagcaggccg agcgccaggc cgacgagatc
     2401 gtcgaggacg cccgcgcccg cgagcgcgag atccgcctcg gcgccgagga ctacgccgac
     2461 gacatcctca acaccctcga gatcaacctc gagaagttca ccgctgccgt gcagcgcggg
     2521 cgcgaccggc tccagcgccg cgaagagcct gccgacgtgg cctagcgcca ctagcctggg
     2581 gtctgatggc ccccctgacc gacacgttcg acctcggcac gcttcgcctg gcctcgggcg
     2641 agggcgccca cgtggacctg gaggtggccg tcgagccgct ggagttcggc ggccagacct
     2701 acaccgtgcc cggcgaccgg gtggccgtcc gcctggacgt gtccaggccc acgggctcgg
     2761 ggttcgccct ccggctgcgc ttcgaggcgc ccctggacgg gccctgcatg cgctgcctcg
     2821 agaacgccga cgccgtgctg gcggtcgacg cgcgtgaggt ggaccagccc ggcggcggcg
     2881 acgagctctc ctcgccctat ctcggcgacg agcaggagct ggacgtgcac ggctgggccc
     2941 gcgacgccct ggcgctggcg ctgcccaccc agatcgtctg ccgcgaggac tgcgcgggcc
     3001 tgtgccccga atgcggcgag aacctgaacc accaccccga ccacgcgcac gagcccgagc
     3061 cggacccccg ctgggccaag ctctcagagc tcaagctgga ctgaaccgtc tacactcggc
     3121 cgctgccatg gccgttccca agcaacgaca gtcttccgcc cgcaccaaca agcggcgctc
     3181 ccagcacaag atcgaggccc cgagcctggc cgtgtgcccg cgctgcggcg ctccccgcat
     3241 cccgcaccgc gtgtgcggcg agtgcggcac ctacgcgggt cgcgagatct tccgccccgc
     3301 ggtcgacgag accgacgacg agtaaccgcg gccccgtgat cgcggtcgac gcgaacgggg
     3361 cagacgcagg gccggcggtg gtcgccgagg gcgcgcgcct gtccggcgag cccacgctcg
     3421 tcttcgggca cgggctgccc gccggcgacg ggatcgtcga ggcccccggc gtccccgcat
     3481 ccgacaaccc ggtccgggcc gtgcgctcga acccggacgc ctcgatcgtc caggccgccg
     3541 ctgcggtggc cgacggacgc gccgaggcgc tggtctcggc cggcagcacc ggccccacgc
     3601 tggccgccgc cacgctggcc atcaagcgcc tcaagggcgt ccaccgcccg gcgctggccg
     3661 tgctggtgcc gatcccggcc aagcccgtgc tgctgctgga ctgtggcgcc aacgtggagg
     3721 tgcgccccga gcacctggtc cagttcgcct acatgggctc cgcgttcatg gaggtcgtga
     3781 tgggggtgga gcggccgagc gtcggcctgc tgtcggtcgg cgaggagagc ggcaagggca
     3841 cgcccgacgt gctcgaggcg cgcgagcgac tggagggcgg gccgctgaac ttcaccggca
     3901 acctcgaggg cttcgacctg cccggcgccg gcgccgacgt ggtcgtggcc gacggcttca
     3961 ccggcaacat cgccctgaag gtgctggagg gcacctcgcg ggtggtgcgc gacgccatcc
     4021 gcgacgccat cacctcgggc ccggtgtcca gcgccggcgg gctcctcatt cgcggcgcgg
     4081 tcggccgcct gcgcgactcg atcgaccccg agaacgtggg cggcgcgatc ctgctgggcc
     4141 tgcgcaagcc ggtggtcgtg ggccacggca gcttcggccc cagggggatc gccagcgccg
     4201 tggccctggc ccgccgggcg gttgacgagg acatggtggg gcgcacgcac gcggcgctgg
     4261 aggccgcggg cgcattgcgg tccgcacccg ctggtagctt cgccaccgtg aagcccgaga
     4321 cccccggaac gccgggctga acgccggcct gccatggaac gcgaagaggt catgaggcgc
     4381 atccgcgagc acctggcggc cgagctcgag gtcgacgcgt cggggatcac cgacgccacc
     4441 cggttcaagg aggacctgga ggccgattcg ctcgacctgg tggagctcac cgtggagctc
     4501 gaggacacct acggcatccg gatccccgac gaggaagcgg cccgcatcct cacggtcgga
     4561 caggccgccg actacgtggc ggcacatgcc gacgacgccg agagctgaga ccggcgagag
     4621 ccttggcgac cttctcgatc agctccccga ggacctggcc cgccaggcct tcacgcacgc
     4681 gtcgtgggtc gaccaccgcg ccgagtccta cgagcgcctg gccttcctgg gggacgtcgt
     4741 cctgagcctc gccgtctcca cccacctgta cccgcgcttc gagcgctacg gcgccggccg
     4801 gctgaccaag gtgcgcgcgc aggccgtcag cggggcctcg tgcgcggcgg tcgcgctcga
     4861 cctcgaggtg ccacggcgcc tgggcgaacg ggcgcccgac ggcggcgccg gcccc
//
LOCUS       DASUPW010000470         4154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_662377, whole genome shotgun sequence.
ACCESSION   DASUPW010000470 DASUPW010000000
VERSION     DASUPW010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662377"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..712)
                     /gene="hpt"
                     /locus_tag="VFY44_10545"
     CDS             complement(179..712)
                     /gene="hpt"
                     /locus_tag="VFY44_10545"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX5910924.1"
                     /translation="MKDPHIGEILVQQDELAHRVDVLAKQISADYAGERLLLVGVLKG
                     AMFFLSDLMRRLDVDCEVDFMAVASYGSSTDSSGVVRILKDLDASIEGREVLIIEDIV
                     DSGLTLSYLLRTLKARDPASVEVCALLEKPERRKVDLPIRYSGFEIPNKFVIGYGLDH
                     AERYRNLPYVAVLEGAV"
     gene            complement(709..1983)
                     /gene="tilS"
                     /locus_tag="VFY44_10550"
     CDS             complement(709..1983)
                     /gene="tilS"
                     /locus_tag="VFY44_10550"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX5910925.1"
                     /translation="MAEPALDAARASGLVPAGRPLLVMLSGGADSVCLLEVALRLDAR
                     VSAVHVNYGLRAAAEQDEAHCRALCDRLGVELTVERAWLPEEGNVHAHARDARYAVAE
                     RVAGGDYAAAHTASDQAETVLYRLAVSPGRRALLGMEPRRGRLVRPLLGATRSDTLDY
                     CRRRGLPWREDASNDDPRFARARVRNALLPALLEVAPAAEGAVAETARQLREEAEVLD
                     AVAAEALAGLGGGPAVELAALRALPRATARLVLRALAEQAAGGPFSLTRERVDAVLGL
                     DAAGSSTAELGDGLEALSEYGTLRIRRTRGDSGPPDPAPLPVPGSARFGDWDVRAGAV
                     GEVTVSVDGPLTVRAWRQGDRIRPVGLGGTKTLADLFTDRKVPRELRRSLPVVVSGDD
                     VVWVAGVALDERFAADEGAEGAVGLSASLSRP"
     gene            complement(1987..3192)
                     /locus_tag="VFY44_10555"
     CDS             complement(1987..3192)
                     /locus_tag="VFY44_10555"
                     /inference="COORDINATES: protein motif:HMM:NF028011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX5910926.1"
                     /translation="MPERTLTLNEAAGIAGVSSDTLRRWLRQGLVPQMKGRKPSAWTP
                     EAASHARLVARLRERGHKLDQIAAATEEGRLASHQLEQLFPDAHGDRTVDDAAELTGL
                     EPALIERFWVSLGLPTQGLETMTDEDLQAVQYAATMLSTGFPLVAFLQLARVYGQALR
                     QIADAEVRLFHLYVHEPLMREGVPGIEMAEQMEHLARDLLPMASPLMDFVHQRFLQHY
                     MEQDVVGHMEVDREEGEDLGRVRVGIAFCDLAGYTRYTEEEGEDEAVSFVERFVEQVT
                     DTLPDDARVIKTIGDEVMIVGPDVQGMTDWAVGFQSLFTERPEPRVGIHYGATLYRDG
                     DYFGREVNTASRVVARSRGGEVLVTDAVVDRVSESDHLVFENVGQVKLKGFDEPRQLF
                     RAATPPDEG"
     gene            3318..>4154
                     /locus_tag="VFY44_10560"
     CDS             3318..>4154
                     /locus_tag="VFY44_10560"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5910927.1"
                     /translation="MNYFVTGATGFIGRHLVEELLRRDGTIYVLVREGSRGKVDALVQ
                     SLGADEGRIVPVAGDLFKEKLGVEGFDEKIDHLFHLAAVYDMEADEEASEKANVQGTR
                     NVIEFANSHDVGRFHHTSSIAVAGSYKGVFQEDMFDEEQKLPHHYHRTKYESEKMVRD
                     GVKAKTLVFRPGIVVGHSRTGEMDKVDGPYYMFTLLKKLRGALPAWMPLAGPEGGKIT
                     IVPVDFVARAMDHIGHLPDEDLPGDTFHLVDPEPMTVGEALNTFAKAAHAPQLAMRVD
                     ENL"
BASE COUNT          622 a         1541 c         1432 g          559 t
ORIGIN      
        1 agcacgatca cgatcaggat cggaaacgcc gcgctcttga agaatctgct catgagatag
       61 aaaaagccgc gtggcggctg aaaccgaagc gtagcaaggg ggtagcagcc ggttcggggg
      121 gcatcctgcc cccctcacac acgtcaactg accttctctc cccctccttc tccgtctttc
      181 agaccgcccc ttccaggacc gccacgtagg gcaggttcct gtaccgctcc gcgtggtcga
      241 ggccgtagcc gatcacgaac ttgttcggga tctcgaaacc cgaatagcgg atcggcaggt
      301 ccaccttgcg ccgctccggc ttctcgagca gggcgcagac ctcgaccgag gcgggatcgc
      361 gcgccttcag cgtgcgcagc aggtaggaca gggtcaggcc cgagtccacg atgtcctcga
      421 tgatcagcac ctcgcggccc tcgatcgacg cgtccaggtc cttgaggatg cgcaccacgc
      481 cgctggagtc ggtggacgat ccgtacgagg ccacggccat gaagtccacc tcgcagtcca
      541 cgtcgagccg cctcatcagg tccgacagga agaacatggc gcccttgagg acaccgacca
      601 gcagcagccg ctcaccggcg tagtccgcgg agatctgctt cgccagcaca tcgacgcggt
      661 gcgccagctc gtcctgctgg acgaggatct cgccgatgtg gggatccttc acgggcgcga
      721 gaggctagcg gacaacccca ccgccccctc ggcgccctcg tccgcggcga agcgctcgtc
      781 cagggccacg cctgccaccc agaccacgtc gtcgccgctg accaccaccg gcaggctgcg
      841 gcgcagctcg cgcgggacct tgcggtcggt gaacaggtcg gccagcgtct tggtcccgcc
      901 caggcccacc gggcggatgc ggtcgccctg gcgccacgcg cgcacggtca gcgggccgtc
      961 gacggacacc gtgacctcac ccacggcgcc cgcgcgcacg tcccagtccc cgaagcgcgc
     1021 cgaaccggga accggcagcg gagccgggtc cggcgggccc gagtcgccgc gcgtgcgccg
     1081 gatccgcagc gtcccgtact cggacagggc ctccaggccg tcaccgagct cggcggtcga
     1141 gctgccggcg gcgtccaggc ccagcaccgc gtcgacgcgc tcgcgggtga gcgagaacgg
     1201 cccgccggcg gcctgctcgg ccagcgcgcg cagcaccagc cgcgcggtgg cgcggggcag
     1261 cgcgcgcagt gcggccagct ccacggccgg ccctccaccc aggccggcca gcgcctcggc
     1321 ggccaccgcg tccaggacct cggcctcctc gcgcagctga cgggcggtct cggccaccgc
     1381 cccctccgcc gcgggcgcga cctcgagcag cgccggcagg agcgcgttcc tcacgcgcgc
     1441 gcgggcgaag cggggatcgt cgttggaggc gtcctcgcgc cagggcaggc cgcggcggcg
     1501 gcagtagtcg agcgtgtccg agcgggtggc gcccaggagc ggccgcacca gccgcccgcg
     1561 ccggggctcc atgcccagca gggcgcgccg gcccggcgag accgccagcc ggtagagcac
     1621 cgtctcggcc tggtcggacg cggtgtgcgc ggcggcgtag tcgcctccgg ccacccgctc
     1681 ggccaccgcg taccgggcgt cccgggcgtg ggcgtgcacg ttgccctcct cgggcagcca
     1741 cgcgcgctcc acggtcagct cgacccccaa tcggtcgcac agcgcgcggc agtgggcctc
     1801 gtcctgctcg gcggcggcgc gcaggccgta gttcacgtgc accgcggaca cccgggcgtc
     1861 caggcgcagc gccacctcca gcaggcagac cgagtcggcg ccgccggaga gcatgaccag
     1921 cagcggccgc ccggcgggca ccagcccgct ggcccgggcc gcatccagcg ccggctcggc
     1981 cacggctcag ccctcgtccg ggggcgtcgc cgcccggaac agctggcgcg gctcgtcgaa
     2041 gcccttgagc ttgacctggc cgacgttctc gaacaccagg tggtcggact ccgagacccg
     2101 gtcgacgacc gcgtcggtga ccagcacctc gccgccgcgc gaccgggcca ccacgcgcga
     2161 cgcggtgttg acctcgcggc cgaagtagtc gccgtcgcgg tacagcgtgg cgccgtagtg
     2221 gatgcccacg cgcggctcgg ggcgctcggt gaacaggctc tggaacccca ccgcccagtc
     2281 ggtcatgccc tgcacgtccg gcccgacgat catcacctcg tcgccgatgg tcttgatcac
     2341 gcgcgcgtcg tcgggaagcg tgtcggtcac ctgctcgacg aagcgctcca cgaacgagac
     2401 tgcctcgtcc tcgccctcct cctcggtgta ccgggtgtag cccgccaggt cgcagaatgc
     2461 gatccccacg cggacgcggc ccaggtcctc cccctcctcg cgatcgacct ccatgtggcc
     2521 caccacgtcc tgctccatgt agtgctggag gaagcgctgg tgcacgaagt ccatcagcgg
     2581 cgaggccatc ggcagcaggt cgcgggccag gtgctccatc tgctcggcca tctcgatgcc
     2641 cggcacgccc tcgcgcatca gcggctcgtg cacgtacagg tggaacagcc gcacctcggc
     2701 gtcggcgatc tggcgcagcg cctggccgta cacgcgcgcc agctgcagga aggccaccag
     2761 cgggaagccg gtggacagca tcgtcgccgc gtactgcacc gcctggaggt cctcgtcggt
     2821 catggtctcc aggccctggg tgggcaggcc caggctgacc cagaaacgct cgatcagcgc
     2881 gggctccagg ccggtcagct cggcggcgtc gtccacggtg cggtcgccgt gggcgtcggg
     2941 gaagagctgc tcgagctggt gcgaggccag ccgtccctcc tccgtcgccg cggcgatctg
     3001 gtcgagcttg tgcccgcgct cgcgcagccg ggccaccagg cgggcgtggc tggccgcctc
     3061 cggggtccat gcagagggct tccggccctt catctgggga accaggccct gccgcagcca
     3121 gcggcgcagg gtgtcggagg acaccccggc gatccctgcg gcctcgttga gcgtcagcgt
     3181 tcgttcgggc acgccccgcg caagctactt cacccgcggg atccctaccg ggggctcgcg
     3241 cgccagggcc ccgcgcgccc tcggctgcgc gcgggatatg ctcgcgccgg ccaaagaggg
     3301 aagagagagg cttcgttgtg aactacttcg ttaccggcgc gaccggtttc atcgggcgcc
     3361 acctcgtcga ggagctgttg cggcgcgacg ggaccatcta cgtgctggtg cgcgagggct
     3421 cgcgcgggaa ggtggacgcg ctggtccaga gcctgggcgc cgacgagggg cggatcgtcc
     3481 ccgtggccgg cgacctgttc aaggagaagc tgggggtcga gggcttcgac gagaagatcg
     3541 accacctgtt ccatctggcc gcggtgtacg acatggaggc cgacgaggag gcctcggaga
     3601 aggccaacgt ccaggggacc cggaacgtca tcgagttcgc caactcacac gacgtcgggc
     3661 gcttccatca caccagctcg atcgccgtcg ccggctccta caagggcgtg ttccaggagg
     3721 acatgttcga cgaggagcag aagctgcccc accactacca ccgtacgaag tacgagtcgg
     3781 agaagatggt gcgcgacggc gtcaaggcca agacgctcgt gttccggccg ggcatcgtcg
     3841 tcggccactc ccggacgggc gagatggaca aggtcgacgg cccgtactac atgttcacgc
     3901 tgctcaagaa gctgcgcggc gcgctgcctg cctggatgcc gctagccggc ccagagggcg
     3961 gcaagatcac gatcgttccg gtcgacttcg ttgcccgtgc gatggatcac atcggccacc
     4021 tgcccgacga ggacctgccg ggcgacacct tccacctcgt cgaccccgag ccgatgaccg
     4081 tcggcgaggc gctcaacacc ttcgcgaagg cggcgcacgc gccccagctc gcgatgcgcg
     4141 tcgacgagaa cctg
//
LOCUS       DASUPW010000471         2696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_109025, whole genome shotgun sequence.
ACCESSION   DASUPW010000471 DASUPW010000000
VERSION     DASUPW010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109025"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /gene="rlmD"
                     /locus_tag="VFY44_10565"
     CDS             <1..921
                     /gene="rlmD"
                     /locus_tag="VFY44_10565"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEX5910928.1"
                     /translation="FHHPGRWDVIDPVEHDVLASERVDALREQVLAWCREAGLSAYDR
                     RDHGGFLRNLVVREGRRTGQLQARLVTSQGAFEKDLFAAAIDADSVLWTQAAGVAETT
                     RDGKTRKLKGGELVAEELTVRDTTLRFGISADAFFQTNTEMAEVLYATALEAAALTGR
                     ERVFDLYCGIGTISLAMALDAADVVGVESVERAVADAIENARANGVDNARFYAGDVRT
                     AMRPLLEEEGSADVVVLDPPRAGLSQKVVRRVLEVEAQKIVYVSCNPTTLAPNARQIV
                     DAGYEMGPVTPVDMFPQTPHIECVTSFSRR"
     gene            894..2402
                     /locus_tag="VFY44_10570"
     CDS             894..2402
                     /locus_tag="VFY44_10570"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5910929.1"
                     /translation="MRHVLLAALTALAVAGPATAPAQAAQKARPPVVMIVLDEFPLAD
                     IQAADGTIDRRRFPGFAALAAGSTWFPNAYAVHDSTHFAVPAILTGRAPRAGADAPTY
                     LTHPRSLFTYLDRLGYRIHSREEATTVCPPRLCPRADRYGNPHYNILHRRRERLDRTI
                     DGLRRTRKPTLTFHHSVLPHVPWSYLPTGQARQGYREGTLPDFASPAGFGDEFLTLFN
                     EQRHLLQAGFADAEVGRLVTRLKKTRQWKDALVVVTADQAISFQRGVSDRRQVTADNV
                     HEVAPVPLFVKRPGQTRGARSTAYVSGLDILPTVAGLLRRPLGWRSDGASVFGRAAQA
                     RRQVRMFRRDLSGLVTVPAAQMEARRANDRLHRLSLFGNGRWSRVFRIGPNRRLLGRT
                     AGHTASPARGAPRARFAVPGNLRRVDPAGAEVPTFASGRLEGGSTEGGRDVALSVNGR
                     VAAVGRSFHLAGDPTEWFALNLPVSALRKGRNAMSLYAVEPDLRLTALGGVP"
     gene            complement(2482..>2696)
                     /locus_tag="VFY44_10575"
     CDS             complement(2482..>2696)
                     /locus_tag="VFY44_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910930.1"
                     /translation="EEALEELNGLGVPLGATVEATPGEIQKLTPSCDALYVPDIDASL
                     DDESAKRDFWRAFRALGDWYADFPPY"
BASE COUNT          361 a         1022 c          970 g          343 t
ORIGIN      
        1 ttccaccacc cgggccggtg ggacgtgatc gaccccgtcg agcacgacgt cctggcctcc
       61 gagcgcgtgg acgcgctgcg cgagcaggtc ctggcctggt gccgcgaagc ggggctcagc
      121 gcgtacgacc gccgcgacca cggcggcttc ctgcgcaacc tcgtggtccg tgagggccgg
      181 cgcaccggcc agctgcaggc ccggctggtc accagccagg gcgcgttcga gaaggacctg
      241 ttcgccgccg ccatcgacgc cgactcggtg ctgtggaccc aggccgccgg cgtggccgag
      301 accacgcgcg acggcaagac gcgcaagctc aagggcggcg agctcgtggc cgaggagctg
      361 acggtccgcg acacgacgct gcgcttcggc atctccgccg acgccttctt ccagaccaac
      421 accgagatgg ccgaggtgct gtacgccacc gcgctcgagg ccgccgcgct gaccggacgg
      481 gagcgcgtgt tcgacctcta ctgcggcatc ggcacgatct cgctggccat ggccctggac
      541 gccgccgacg tggtgggcgt cgagagcgtc gagcgcgccg tggccgacgc catcgagaac
      601 gcgcgcgcca acggcgtgga caacgcgcgc ttctacgcgg gcgacgtgcg caccgcgatg
      661 cggccgctgc tggaggagga gggcagcgcc gacgtggtgg tgctggatcc gccccgcgcc
      721 ggcctgtcgc agaaggtggt gcgccgggtg ctggaggtcg aggcgcagaa gatcgtctac
      781 gtgtcgtgca accccaccac gctggcgccc aacgcgcgcc agatcgtgga cgcgggctac
      841 gagatggggc ccgtgacccc ggtcgacatg ttcccgcaga ccccgcacat cgaatgcgtc
      901 acgtccttct cgcggcgctg accgcgctcg cggttgccgg cccggccacc gccccggccc
      961 aggcggcaca gaaggcccgg ccaccggtcg tgatgatcgt gctcgacgag ttcccgcttg
     1021 ccgacatcca ggctgcggac ggcacgatcg accggcggcg gttccccggg ttcgccgcgc
     1081 tggccgcggg ctcaacctgg ttccccaacg cctacgcggt gcacgacagc acgcacttcg
     1141 cggtgccggc catcctgacc gggcgcgcgc cccgcgcggg agccgacgcc cccacctacc
     1201 tcacccaccc gcgctcgctg ttcacctacc tggaccgcct gggctaccgg atccactcgc
     1261 gcgaggaggc cacgaccgtg tgcccgccgc gcctgtgccc gcgcgccgac cgctacggca
     1321 acccgcacta caacatcctc caccgcaggc gcgagcgcct ggaccgcacc atcgacgggc
     1381 tgcgccggac caggaagccc acgctgacct tccaccactc ggtgctgccg cacgtgccct
     1441 ggagctacct gcccacgggc caggcgcgcc agggctaccg cgaggggacc ctgcccgact
     1501 tcgcctcgcc ggccggcttc ggcgacgagt tcctgacgct gttcaacgag cagcgccacc
     1561 tgctgcaggc ggggttcgcc gacgccgagg tgggccggct ggtgacccgg ctgaagaaga
     1621 cgaggcagtg gaaggacgcg ctggtggtgg tcaccgccga ccaggcgatc tcgttccagc
     1681 ggggcgtgag cgaccggcgc caggtcaccg cggacaacgt ccacgaggtg gcgccggtgc
     1741 cgctgttcgt caagcgcccc ggccagaccc gcggcgcccg ctccaccgcc tacgtcagcg
     1801 ggctggacat cctgcccacc gtcgccgggc tcctgcgccg gccgctgggc tggaggagcg
     1861 acggcgccag cgtgttcggc cgcgccgcgc aggcgcggcg ccaggtgcgc atgttccggc
     1921 gcgacctgtc cggcctggtc accgtgcccg ccgcccagat ggaggcccgc cgggcgaacg
     1981 accggctgca ccgcctgtcc ctgttcggca acggccgctg gtcgcgcgtc ttccggatcg
     2041 ggccgaaccg ccggctgctg ggccgcacgg ccggccacac cgcctcaccg gcgcgggggg
     2101 cgccgcgggc ccggttcgcg gtccccggga acctgcgccg cgtcgatccc gccggcgccg
     2161 aggtgcccac gttcgcctcc gggcgcctgg agggaggctc caccgagggc ggccgcgacg
     2221 tggcgctgtc ggtgaacggc cgcgtcgccg cggtgggccg cagcttccac ctggccggcg
     2281 acccgaccga gtggttcgcg ctcaacctgc ccgtgtcggc gctgcgcaag gggcgcaacg
     2341 ccatgagcct gtacgcggtc gagccggacc tgcggctgac ggccctgggc ggagtccctt
     2401 aggaggccgg cgaagccggc cggtctcgca gcctggatgg ctgcgtgaag atcaaggcgc
     2461 agccacggat ggctgcaagg gctagtacgg cgggaagtcg gcgtaccagt cgccgagggc
     2521 ccggaaggca cgccagaagt cgcgcttggc ggactcgtcg tccagcgagg cgtcgatgtc
     2581 gggcacgtac agcgcgtcgc agctgggggt cagcttctgg atctcgcccg gggtggcctc
     2641 gacggtcgcg cccagcggca cgcccaggcc gttcagctcc tccagcgcct cctcgc
//
LOCUS       DASUPW010000472         2904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1910358, whole genome shotgun sequence.
ACCESSION   DASUPW010000472 DASUPW010000000
VERSION     DASUPW010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1910358"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..652
                     /locus_tag="VFY44_10580"
     CDS             170..652
                     /locus_tag="VFY44_10580"
                     /inference="COORDINATES: protein motif:HMM:NF025384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HEX5910931.1"
                     /translation="MAEPIEIQGSTYTGKIRNPLAVIGLSLITFGIYGIFWYYYANKE
                     LAEIGRAHNTDELGDSPGTSVLAITLGAFVIVPALVSAYKFCKRLQTAGRLTGAGEGM
                     EAGLLFIIYLFVSPVGAYIAQSNLNKVLQAQAGGAGSLGAGSGVTSPEIPVAPPAQQQ
                     "
     gene            complement(657..1307)
                     /locus_tag="VFY44_10585"
     CDS             complement(657..1307)
                     /locus_tag="VFY44_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910932.1"
                     /translation="MPDAQLDDAYQRQLSHWRAGLDSGAHRIGWKVGLNPPKVQEALG
                     LSSPVVGHLTSATLLGADGAHSLAGAQAPKAEPEIVIEIGPDKTIAGLGPAIELVDIP
                     ALPSGPDDVPEVVATNIFHRGVAIGSSAAVDSPAGATWSLSVDGEVVREGDASDYPIA
                     DMIHAVADTLEAAGEHLEAGDRIIAGALAPPPDVEAGQRLELDLGPLGAIEVQLTV"
     gene            complement(1300..2286)
                     /locus_tag="VFY44_10590"
     CDS             complement(1300..2286)
                     /locus_tag="VFY44_10590"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX5910933.1"
                     /translation="MTETVTATPATDSRAPVPGADDIQPTANETLDRFLTGTITVLPF
                     VGLGLACWQLWGSALHWHDVVVFAVMYLITGLGITVGFHRLFTHRAFQTKPWMRGVIA
                     AAGSMAIEGPITAWVADHRKHHAFSDQHGDPHSPHVDHGHGLKGALRGLWHAHWGWLF
                     IHTQRGNKQRYARDLIDDPVVSWVDRTFLLWVITGLVLPFLLGWAIGGTLFAAFTGLL
                     WGGLIRMLVLHHMTYSINSLCHFFGRRDYETDDQSRNLLWLAPFTLGESWHNNHHAFP
                     TSAAHGLRWWQLDISAGVIWVMEKTGLAWDVKRIPRERQQAKANGEAKAASA"
     gene            2419..>2904
                     /locus_tag="VFY44_10595"
     CDS             2419..>2904
                     /locus_tag="VFY44_10595"
                     /inference="COORDINATES: protein motif:HMM:NF014998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX5910934.1"
                     /translation="MAKHMDRLSSVDAAFLSQEREGSHMHIGSILLFEGPAPRPEEVT
                     AHVESRLHLVPRYRHKLSFPRLEMGRPLWVDDPSFNIGYHVRQTALPAPGSVEQLRLL
                     AGRIFSQRLDRSKPLWELWLVEGLEDGRFAVINKTHHSLVDGVSGADLTAVMFDLERD
                     GT"
BASE COUNT          434 a          998 c         1038 g          434 t
ORIGIN      
        1 tgtgaccagt gccttcatcg tcctggttac gtacccggat cggggacggg ccaccggaca
       61 gatgtccacc aagattcgcc gtccgccgaa ggctgggtta ccttgatctc gcgctgccga
      121 ggccgaaggt gctggaaatg cggcgaatca accggaaaag gagccgcaca tggcagagcc
      181 gatcgagatt cagggatcca cgtacacggg caagatccgc aacccgctgg cggtcatcgg
      241 gctgagcctg atcaccttcg ggatctacgg gatcttctgg tactactacg ccaacaagga
      301 gctcgccgag atcggcaggg cccacaacac cgacgagctc ggcgactcgc ccggcacctc
      361 ggtcctggcc atcaccctcg gcgccttcgt catcgtgccg gcgctcgtgt cggcctacaa
      421 gttctgcaag cgcctccaga ccgccggccg gctgaccggc gcgggcgagg gcatggaggc
      481 agggctgctc ttcatcatct acctgttcgt ctcgcccgtg ggcgcctaca tcgcgcagtc
      541 gaacctgaac aaggtgctgc aggcgcaggc cggcggagcc ggttccctgg gcgccggcag
      601 cggggtgacc tctccggaga tccccgtcgc gccgcccgcg cagcagcagt agcgcttcag
      661 acggtgagct ggacctcgat cgcccccagc gggccgaggt ccagctcgag ccgctgaccc
      721 gcctccacgt cgggcggcgg cgccagcgcg ccggcgatga tccggtcgcc ggcctccaga
      781 tgctcacccg cggcctccag cgtgtcggcc accgcgtgga tcatgtcggc gatcgggtag
      841 tcggaggcgt cgccctcccg caccacctcg ccgtccacgc tgagcgacca cgtggccccc
      901 gcgggggagt ccacggccgc ggaggagccg atggccacgc cgcggtggaa gatgttggtg
      961 gccaccacct cgggcacgtc gtccgggccc gacggcagcg ccgggatgtc aacgagctcg
     1021 atggcggggc ccaggccggc gatggtcttg tcgggcccga tctcgatcac gatctcgggc
     1081 tcggccttgg gcgcctgcgc gcccgccagc gagtgggcgc cgtcggcgcc cagcagggtg
     1141 gcggaggtca ggtggccgac caccggcgag gacaggccca gcgcctcctg caccttgggc
     1201 gggttcaggc cgaccttcca gccgatccgg tgcgcgccgc tgtccagccc ggcgcgccag
     1261 tggctcagct ggcgctggta ggcgtcgtcg agctgggcgt caggcactgg cggccttggc
     1321 ctcgccgttg gccttggcct gctggcgctc gcgcgggatg cgcttgacgt cccacgccag
     1381 gccggtcttc tccatcaccc agatcacgcc ggccgagatg tccagctgcc accagcgcag
     1441 gccgtgcgcg gcggaggtgg ggaaggcgtg gtggttgttg tgccacgact cgccgagcgt
     1501 gaacggggcc agccagagca ggttgcgcga ctggtcgtcg gtctcgtagt cgcgccggcc
     1561 gaagaagtgg cacagcgagt tgatgctgta ggtcatgtgg tgcagcacga gcatccggat
     1621 caggccgccc cagagcaggc ccgtgaacgc ggcgaacagc gtccccccga tggcccagcc
     1681 cagcaggaac ggcagcacca ggcccgtgat cacccagagc aggaacgtgc ggtcgaccca
     1741 gctgaccacc gggtcgtcga tcaggtcgcg ggcgtagcgc tgcttgttgc cgcgctgggt
     1801 gtggatgaac agccagcccc agtgcgcgtg ccacaggccg cgcagggcgc ccttgaggcc
     1861 gtgcccgtgg tccacgtggg ggctgtgggg atcgccgtgc tgatcggaga aggcgtggtg
     1921 cttgcggtgg tcggccaccc aggcggtgat cgggccctcg atggccatcg agcccgccgc
     1981 ggcgatgacc ccgcgcatcc acggcttggt ctggaacgcg cggtgcgtga acaggcggtg
     2041 gaagccgacg gtgatgccga ggccggtgat caggtacatc accgcgaaca cgaccacgtc
     2101 gtgccagtgc agggcgctgc cccagagttg ccagcaggcc agccccaggc cgacgaacgg
     2161 caggacggtg atggtgccgg tcaggaagcg gtccagcgtc tcgttggcgg tcggctggat
     2221 gtcgtcggcg ccgggcacgg gcgcacgcga atcggtcgcg ggcgtggctg tcaccgtctc
     2281 ggtcaccggg cggagagtac ccgtcggcca ccgatccgga acggcttcct taatccgtga
     2341 accgctttgc gggtgtaggt tcgacagcgg cggccgtgcg ggagcggccg ctctggaacc
     2401 ggaaaggagg agatggcgat ggcgaagcac atggatcgct tgagctcggt ggacgccgcg
     2461 ttcctgtcac aggagcgcga gggctcgcac atgcacatcg gcagcatcct cctgttcgag
     2521 ggccctgcgc cccggcccga ggaggtcacc gcgcacgtgg agtcgcgcct gcacctggtg
     2581 ccccgctacc gccacaagct ctccttcccg cgcctggaga tgggccgccc gctgtgggtc
     2641 gacgacccca gcttcaacat cggctaccac gtgcgccaga ccgcgcttcc cgcgccgggc
     2701 tcggtcgagc agctgcggct tctggccggc cggatcttca gccagcgcct ggaccggtcc
     2761 aagccgttgt gggagctgtg gctcgtcgag gggctcgagg acgggcgctt cgcggtcatc
     2821 aacaagaccc accacagcct ggtggacggc gtctccggcg ccgacctgac cgcggtcatg
     2881 ttcgacctcg agcgcgacgg cacc
//
LOCUS       DASUPW010000473         2993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_388692, whole genome shotgun sequence.
ACCESSION   DASUPW010000473 DASUPW010000000
VERSION     DASUPW010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388692"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..369)
                     /gene="rbfA"
                     /locus_tag="VFY44_10600"
     CDS             complement(28..369)
                     /gene="rbfA"
                     /locus_tag="VFY44_10600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX5910935.1"
                     /translation="MAGERMRRVDEAVREVLSQRLTAGLKDPRIGFVTVTSVETAPDL
                     RNARVYVSVLGSEEERRDTLEGLQSSSGFLQAALGRELRMKRTPTLEFVHDDSVDRGF
                     RIGEIIEEHDR"
     gene            complement(373..774)
                     /locus_tag="VFY44_10605"
     CDS             complement(373..774)
                     /locus_tag="VFY44_10605"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEX5910936.1"
                     /translation="MRHRARELRRREAGRHPRGLRDPAGRADPRVAIAFLVYLELGLF
                     FPEGSDLKGKRKEVQSVKAHLSRRFGASVAETDHHDLWQRATLSCALVARDASGARSQ
                     ADALERWLSGRFPEGYSVTRALVSGDELVEG"
     gene            complement(681..2852)
                     /gene="infB"
                     /locus_tag="VFY44_10610"
     CDS             complement(681..2852)
                     /gene="infB"
                     /locus_tag="VFY44_10610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX5910937.1"
                     /translation="MAKKRVHEIAKERGIPSKEVLATLQAAGLDVKAAASSVDERDIA
                     RAFNPSAQEAASEEPSGNGDSASADAPAAQRPTRTEGGGGSAKRRRVVIDSQASRRAP
                     GPPPQQNQPPRRRGRRRRTPWVEPDLTAKPDAPEEERISAVQSGATVKEVAESLGVAA
                     PEVIKKLMELGEMATLTQTLSDEAIEVLADGLDKKIEIQHAADDVVAEPEYEDDESSL
                     EDRPPVITILGHVDHGKTSLLDAIRETEVAAGEAGGITQHIGAYQVHHGDHTLTFLDT
                     PGHEAFTAMRARGGQVADIAVVVVAADDGVMPQTTEAVDHAKAAEVPIMVAVNKIDKE
                     GADPNRVRGELAQQGLNPADWGGDTEFADVSAKTHQNLDELLDTLITLADIEELKANP
                     DAEASGTVIESRLDPGRGAVVSLLVQRGTLKVGDAVVAGAHSGRARAMHDFRGDKLKE
                     ATPGMPAEILGFDGVPEAGEHFRVVSNEREARRVAGERANRLKTEAIARRAGVRVSLE
                     DVMARARGGELAELNLIVKADVAGSLEALADEIAKLPQEQVTVNLIRDAVGGINESDI
                     MLASASEAIVIGFNVRPVGEAAEVARREGVEIRTYSVIYRVIEELRQAMEGLLAPEEV
                     ENTLATVEVRETFKASRIGTIAGSMVIEGTVTRGNRVRLVRDGTIVYDGRIASLRRFK
                     DDVREVTAGMECGIVLENFGDVKQGDILEVYETQQVEQTLA"
BASE COUNT          363 a         1092 c         1036 g          502 t
ORIGIN      
        1 gcagttgatc gagctctgtt cccacgctca ccggtcgtgt tcctcgatga tctcccctat
       61 acggaacccc ctgtcgacgg agtcgtcgtg cacgaactcc agcgtcggcg tgcgcttcat
      121 ccgcagctcc cgtcccaggg ccgcctgcag gaacccgctc gaggactgaa gcccctcgag
      181 ggtgtcccgt cgttcctcct ccgagccgag cacgctgacg tagacgcgcg cgttgcggag
      241 gtctggggcg gtctccacgg acgtcacggt cacgaatcct atgcgtgggt ccttcaaccc
      301 cgccgtcagc ctctgggaca gcacctcgcg gaccgcctcg tccaccctgc gcatgcgctc
      361 gccggccatg cgtcagccct cgaccagctc gtcgccggag accagcgccc gggtcaccga
      421 gtagccctcg gggaagcgcc cggacagcca ccgctcgagc gcgtccgcct gggatcgggc
      481 gccggaggcg tcacgggcga ccagcgcgca ggacagcgtc gcccgctgcc acaggtcgtg
      541 gtggtcggtc tcggccacgg aggcgccgaa gcgccggctc agatgtgcct tcacggactg
      601 cacctccttg cgctttccct tcaaatcgga cccctcgggg aagaacagcc ccagctcgag
      661 gtagaccagg aacgcgatcg ctacgcgagg gtctgctcga cctgctgggt ctcgtagacc
      721 tcgaggatgt cgccctgctt cacgtctccg aagttctcga gcacgatgcc gcactccatg
      781 cctgcggtga cctcacgcac gtcgtccttg aaccggcgca gcgaagcgat ccggccgtcg
      841 tacacgatcg tcccgtcgcg caccaggcgc accctgttgc cgcgggtgac ggtgccctcg
      901 atgaccatgg agccggcgat ggtgccgata cgggatgcct tgaaggtctc gcgcacctcg
      961 acggtggcca gcgtgttctc gacctcctcc ggggccagca gtccctccat ggcctggcgc
     1021 agctcctcga tcacgcggta gatcaccgag taggtgcgga tctcgacgcc ttcgcgccgc
     1081 gcgacctcgg cggcctcgcc gaccgggcgc acgttgaagc cgatcacgat ggcctcggag
     1141 gccgaggcca gcatgatgtc ggactcgttg atgccgccga cggcgtcgcg gatcaggttc
     1201 accgtgacct gctcctgggg cagcttggcg atctcgtcgg ccagggcctc cagcgagccg
     1261 gccacgtcgg ccttgacgat caggttcagc tcggccagct cgccgccgcg ggcgcgggcc
     1321 atgacgtcct ccagggacac gcgcacgccg gcacggcggg cgatggcctc ggtcttgagc
     1381 cggttggcgc gctcgccggc cacgcgccgg gcctcacgct cgttgctgac cacgcggaag
     1441 tgctcaccgg cctcgggcac gccgtcgaag cccaggatct cggccggcat gcccggcgtg
     1501 gcctccttga gcttgtcgcc gcggaagtcg tgcatggcgc gggcgcggcc cgagtgggcg
     1561 ccggccacga cggcgtcgcc caccttgagc gtgccgcgct gcaccagcag gctgaccacg
     1621 gcgccgcggc ccgggtccag gcgcgactcg atcacggtgc ccgacgcctc ggcgtccggg
     1681 ttggccttga gctcctcgat gtcggccagc gtgatcagcg tgtccagcag ctcgtccagg
     1741 ttctggtggg tcttggcgga cacgtcggcg aactcggtgt cgccacccca gtcggcgggg
     1801 ttcaggccct gctgggccag ctcgcctcgg acgcggttgg ggtccgcgcc ctccttgtcg
     1861 atcttgttga ccgcgaccat gatcggcacc tcggccgcct tggcgtggtc gacggcctcg
     1921 gtggtctgcg gcatgacgcc gtcgtcggcg gccacgacca cgaccgcgat gtcggctacc
     1981 tggccgccgc gggcccgcat ggcggtgaac gcctcgtggc cgggggtgtc caggaaggtc
     2041 agcgtgtggt cgccgtggtg cacctggtag gcgccgatgt gctgggtgat gccgccggcc
     2101 tcgcccgcgg ccacctcggt ctcacggatg gcgtccagca gcgaggtctt gccgtggtcg
     2161 acgtggccga ggatcgtgat gaccggcggg cggtcctcga ggctggactc gtcgtcctcg
     2221 tactcgggct cggccacgac gtcgtcggcg gcgtgctgga tctcgatctt cttgtccagg
     2281 ccgtcggcca ggacctcgat cgcctcgtcc gagagcgtct gcgtcagcgt ggccatctcg
     2341 ccgagctcca tcagcttctt gatgacctcg ggcgcggcga cgcccagcga ctccgcgacc
     2401 tccttgacgg tcgcgccgga ctggaccgcc gagatgcgct cctcctcggg ggcgtcgggc
     2461 ttggccgtca ggtcgggctc gacccagggc gtgcgccggc gccggccgcg gcggcgcggc
     2521 ggctggttct gctgcggcgg cggcccgggg gcccgccgtg aagcctgcga gtcgatgacc
     2581 acgcggcggc gcttggcgct tccgccgcca ccctcggtcc gcgtcgggcg ctgcgctgcg
     2641 ggcgcgtccg cgctcgcact gtcgccgttg ccgctgggct cctcggacgc ggcctcctgc
     2701 gccgaggggt tgaacgcacg cgcgatgtcg cgctcgtcga cgctcgatgc ggcggccttc
     2761 acgtccaggc ccgcggcctg cagggtcgcc aggacctcct tggagggaat cccgcgctcc
     2821 ttcgctatct catgaactcg cttcttcgcc atctcagttg aactctaaag gcctcaggag
     2881 gccccttcgg ccggtgcggg ctcgccgccg ccgccgatct ggtcgccttc ctggtccgcg
     2941 agggcctggt gcgccggtag gccgcagtag cgcgagccgg cgatggccgc gtt
//
LOCUS       DASUPW010000474         2504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_663711, whole genome shotgun sequence.
ACCESSION   DASUPW010000474 DASUPW010000000
VERSION     DASUPW010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663711"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VFY44_10615"
     CDS             <1..457
                     /locus_tag="VFY44_10615"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEX5910938.1"
                     /translation="DLAGGVRPAVPDRIAIPAAKVDTVVDAVGTGPGGAIRVPSLGRA
                     GWYDGGPRPGEPGRAVVIGHLDTKRGPGLFARVPSLRTGEDIRITDRRGRVHDYLVVG
                     RAQVRKDRFPTEEVYGGSGRSVLVLVTCGGPYDRETGYRDNILVYARAA"
     gene            complement(454..930)
                     /locus_tag="VFY44_10620"
     CDS             complement(454..930)
                     /locus_tag="VFY44_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910939.1"
                     /translation="MSDGRHDFDFLHGSWTIRNRRLAARLEGSDDWLEFDSTAQVRPI
                     LLGLGNSETISIPDLPGTGPFEGHTTRLFDPVARHWSIFWASTGAPGRMDPPLTGTFE
                     DGQGVFYGDDTFQGRPVRVRFDWIPDGPDRARWQQSYSDDGGSSWELDWIMDFSRA"
     gene            956..2059
                     /locus_tag="VFY44_10625"
     CDS             956..2059
                     /locus_tag="VFY44_10625"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX5910940.1"
                     /translation="MTPADSISLARGAPSLDIVAVDDLRAAADAAFTKDPAGAFSYGT
                     SAGYKPLVEWIANRQGVAPEQVIATNGSMQADAFLFSALVSPGDLVVVEAPSYDRTLL
                     ALRQLGAEFLAIPLEDDGIDVDALESALEGGARPKLAHIIPNFHNPAGCTLSRAKREK
                     LVALAEQYDFVLFEDDPYVELRFAGESEPTMLSLDTSGHVVYASSFSKTVCPGIRVGY
                     LAGPAELIADIRKQATGVYISPNMVAQSIVAEFCSSGAIERSIETVKNALLERRDVLT
                     AALQRELPDAEFVVPQGGYFLWVELPEGSDVAAIADLAKEKGVVFVKGTDFLLEGGEN
                     ALRIAYSGVPADQIDEAVSRLASAYRELSGAVA"
     gene            2125..>2504
                     /locus_tag="VFY44_10630"
     CDS             2125..>2504
                     /locus_tag="VFY44_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5910941.1"
                     /translation="MALKTAATALACALTVLVAACGSDSGSSGGDADSGTTITHKFGT
                     TKVPENPKRIVTLGYNDHEFLLAMGVEPVAIRDWVGVGPVTKVPWAKDEIAAMKKKPT
                     LIPRTDLKPEQVAKLKPDLIIGVFG"
BASE COUNT          374 a          892 c          883 g          355 t
ORIGIN      
        1 ggacctggcc ggcggcgtca ggccggcggt gcccgaccgg atcgccatcc ccgcggcgaa
       61 ggtcgacacg gtggtcgatg ccgtcggcac cggccccggg ggcgccatcc gcgtgcccag
      121 cctgggccgc gccggctggt acgacggcgg gccgcgcccc ggggagccgg ggcgggcggt
      181 ggtcatcggc cacctggaca ccaagcgcgg gcccggcctg ttcgcccggg tgccctcgct
      241 tcgcacgggc gaggacatcc gcatcaccga ccgccgcggc cgggtgcacg actacctcgt
      301 ggtcggccgc gcccaggtgc gcaaggaccg cttccccacc gaagaggtct acggcggctc
      361 cgggcggtcc gtcctggtgc tggtcacctg cggcggaccc tacgaccgcg agacgggcta
      421 ccgggacaac atcctggtgt acgcgcgggc ggcctaggcg cggctgaagt ccatgatcca
      481 gtccagttcc cacgagctgc cgccgtcgtc ggaatacgac tgctgccagc gggcgcggtc
      541 cgggccgtcg gggatccagt cgaagcggac ccggacgggg cgcccctgga aggtgtcgtc
      601 cccgtagaag acgccctggc cgtcctcgaa cgtgccggtc aggggcgggt ccatgcggcc
      661 gggagcaccg gtcgacgccc agaagatgct ccagtgccgc gcgacgggat cgaacagccg
      721 ggtggtgtgg ccctcgaacg ggcccgtgcc gggcagatcg gggatcgaga tcgtctccga
      781 gttgccgagg cccaacagga tcgggcgcac ctgggcggtg gagtcgaact ccagccagtc
      841 gtcgcttccc tcgaggcggg cggccagccg cctgttccgg atcgtccagc tgccgtgcag
      901 gaagtcgaaa tcgtggcgac cgtcgctcat gagcggccac ggtatcgtca gagcggtgac
      961 ccccgccgac agcatctcac tcgcccgtgg agccccctcg ctggacatcg tggccgtgga
     1021 cgacctgcgc gccgccgccg atgccgcgtt caccaaggac ccggccggcg ccttctcgta
     1081 cggcacgtcg gcgggctaca agccgctggt cgagtggatc gcgaaccgcc agggggtggc
     1141 acccgagcag gtgatcgcca ccaacggatc gatgcaggcg gacgccttcc tgttcagcgc
     1201 gctggtctcg cccggcgatc tggtggtggt cgaggccccc agctacgacc gcacgctgct
     1261 ggccctgcgc cagctgggcg ccgagttcct ggccatcccg ctggaggacg acggcatcga
     1321 cgtcgacgcc ctggagagcg cgctcgaggg cggcgcgcgc ccgaagctgg cgcacatcat
     1381 ccccaacttc cacaaccccg ccggctgcac gctctcgcgc gcgaagcgcg agaagctggt
     1441 ggcgctggcc gagcagtacg acttcgtgct gttcgaggac gacccctacg tggagctgcg
     1501 cttcgcgggc gagtccgagc ccacgatgct gtcgctggac acctcgggcc acgtggtcta
     1561 cgcgtcctcg ttctcgaaga cggtgtgccc cggcatccgg gtcggctacc tggccgggcc
     1621 ggccgagctg atcgccgaca tccgcaagca ggcgaccggc gtgtacatct cgccgaacat
     1681 ggtcgcccag tcgatcgtgg ccgagttctg ctcctcgggc gccatcgagc gctcgatcga
     1741 gacggtcaag aacgcgctgc tcgagcgccg cgacgtgctg accgcggcgc tccagcgtga
     1801 gctgccggac gccgagttcg tggttcccca gggcggctac ttcctgtggg tcgagctgcc
     1861 cgagggcagc gacgtggccg ccatcgccga cctggccaag gagaagggcg tggtgttcgt
     1921 caagggcacc gacttcctcc tggagggcgg cgagaacgcg ctccggatcg cctactcggg
     1981 cgtgcccgcg gaccagatcg acgaggcggt ctcgcggctg gcatcggcct accgcgagct
     2041 ctccggcgcc gtcgcctagg tttagccgac accgaagtta gggaacccta actgcgtccg
     2101 tacccgacgc agactggact tcccttggcc ctgaagaccg cagcaaccgc gctggcgtgc
     2161 gccctgacgg tgctcgtcgc cgcctgtggc agcgactccg gctcctccgg cggcgacgcc
     2221 gattccggca ccacgatcac gcacaagttc ggcaccacca aggtgcccga gaaccccaag
     2281 cggatcgtga cgctgggcta caacgaccac gagttcctgc tggcgatggg cgtcgagccg
     2341 gtcgccatac gtgactgggt cggggtcggg cccgtgacca aggtcccgtg ggccaaggac
     2401 gagatcgccg cgatgaagaa gaagcccacg ctgatccccc ggaccgacct caagcccgag
     2461 caggtggcca agctgaagcc cgacctgatc atcggcgtgt tcgg
//
LOCUS       DASUPW010000475         3710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_250205, whole genome shotgun sequence.
ACCESSION   DASUPW010000475 DASUPW010000000
VERSION     DASUPW010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250205"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VFY44_10635"
     CDS             complement(<1..199)
                     /locus_tag="VFY44_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="involved in the transport or adenine; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEX5910942.1"
                     /translation="MLERHFRLSERGTTVRAEAIGGLATFLTMSYILFVNPAILSAAG
                     MPFGAVAVATALVSCVATATMG"
     gene            248..976
                     /locus_tag="VFY44_10640"
     CDS             248..976
                     /locus_tag="VFY44_10640"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5910943.1"
                     /translation="MPTITLHALAPSHPCMTADAALRLKGLEFERVDFPPGPHIEQMA
                     EIYGQGNTTVPGLTIDDERVHTSRAILARLEELEPEPALYPGDRADAVREAEKWGDEQ
                     LQDLGRRMPWGALHFRPEAMGTFGGAGPLDGPGTDFAIKLIRGTWKYHGLSAELLAND
                     LKGLPAKLDHVDQLAADGVIGTEEATAADLQIGATIRVLMVVEDLLPLIEGRPGEEIA
                     RRWFPDYAGSVPAGAFPAGWVPQR"
     gene            973..1866
                     /locus_tag="VFY44_10645"
     CDS             973..1866
                     /locus_tag="VFY44_10645"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5910944.1"
                     /translation="MTGFDAWDVELHGHRMVYRVAGDGPPVVLIHGMVNNSRHWQSVA
                     LALAERHTVIAPDLIGHGDSESPRVDYSLGGHATRIRDLLASLGIDRASMIGHSYGGG
                     VAMQFFWQFPQRTDRLMLVSAGGLGPEVSPMLRSAALPGASGLLWAIAHPRVVEALTG
                     AGTIGRQVSRALRPLSGPGPRQAFLQTLRSVIDVRGQRVSARDRLYLLSHAPTPTQIV
                     WGGRDHTIPVAHGEFAHRAVPHSRFDVLPEAAHFPHLEDPEGLARVLGEFLEETEPAP
                     VDDADWGDVIAERAPRSRVGA"
     gene            1879..2262
                     /locus_tag="VFY44_10650"
     CDS             1879..2262
                     /locus_tag="VFY44_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910945.1"
                     /translation="MESREASGQGYVEGVSSGERGPIREEAGSTPMPRHPGEALTEPA
                     AVIEPPSTPDGTFVKVRASDHPLPRACEVTVNFQDYHRSSSKLGRFGKIVGIDLDGGE
                     YLVMLDNFECLAAPMRRVEWRKVAT"
     gene            2241..3365
                     /locus_tag="VFY44_10655"
     CDS             2241..3365
                     /locus_tag="VFY44_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5910946.1"
                     /translation="MAQGRDVTAPVSLEELEADPHATLARLRATEPVAWVDALNGWLV
                     TSRALALEVMRDAGRFTVDDPRFTTARVVGPSMLSLDGAEHARHREPWLPRFGRGRVH
                     ERLGPVVTRETDRLLDAVADRGRADLRAALAGPLAAAVVGHALELEADPGTVLGWYRG
                     IVDSVQTMTAGGEPTAAGREAFAELAAALDTDAASDAAVILFGGIETTEGMIATALWY
                     LLREPGGVERALAEPAWLEGAIEESLRLEPAAAMVDRYATADTTLGTAAVRAGDLVAV
                     SVTAANRDPESFPEPDRFDPLRSYAKRHLAFASGPHVCIGVHLARLEAHTAVRRVLER
                     LPALALDPGRSAAPRGLVFRKPPQLEVTWRPEPERSSLRT"
     gene            complement(3311..>3710)
                     /locus_tag="VFY44_10660"
     CDS             complement(3311..>3710)
                     /locus_tag="VFY44_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910947.1"
                     /translation="IDTSRTTMNWARQHTISVKVLVRSDGMPIYVSRGTKRCSGREAP
                     RAGEPLRMHQRSEETRLFIVALLGGQAGAAFGLLFLGGMLFAPLLTALLGSAAGPAFV
                     IGRDRLRAWAGERYVRSELRSGSGRQVTSS"
BASE COUNT          483 a         1383 c         1371 g          473 t
ORIGIN      
        1 ggcccatggt cgcggtggcc acgcacgaga ccagggcggt ggccaccgcc acggctccga
       61 agggcatccc ggcggccgac aggatcgccg ggttcacgaa caggatgtag ctcatggtca
      121 ggaacgtggc caggccgccg atggcctcgg cccgcaccgt cgtaccccgc tcgctcagcc
      181 ggaagtggcg ttcaagcatc gcggcaacct accgggaagc gtcggcaaag ggcatagggt
      241 ccagcgcatg cccacgatca ccctgcacgc gctggcgccc tcccacccct gcatgaccgc
      301 cgacgcggcg ttgcggctca agggcctgga gttcgagcgc gtggacttcc cgccggggcc
      361 gcacatcgag cagatggccg agatctacgg ccagggcaac accacggtgc ccgggctgac
      421 catcgacgac gagcgggtgc acacctcgcg cgcgatcctg gcccggctgg aggagctcga
      481 gcccgagccc gcgctctacc ccggcgaccg cgcggacgcg gtgcgcgagg ccgagaagtg
      541 gggcgacgag cagctgcagg acctcggccg gcggatgccc tggggcgcgc tgcacttccg
      601 gcccgaggcg atgggcacct tcggcggcgc cgggccgctg gacggccccg gcaccgactt
      661 cgccatcaag ctgatccgcg gcacctggaa gtaccacggc ctcagcgccg agctgctggc
      721 caacgacctc aaggggctgc cggccaagct cgaccacgtc gaccagctgg ccgccgacgg
      781 ggtgatcggc accgaggagg ccaccgcggc cgacctgcag atcggcgcga cgatccgggt
      841 gctgatggtg gtcgaggacc tgttgccgct gatcgagggc cgccccggcg aggagatcgc
      901 ccggcgctgg ttccccgact acgccggctc cgtcccggcc ggggcgttcc ccgcggggtg
      961 ggtgcctcag cggtgaccgg cttcgacgcc tgggacgtcg agctgcacgg ccaccggatg
     1021 gtctaccggg tggccggcga cggcccgccc gtggtgctga tccacgggat ggtcaacaac
     1081 tcgcgccatt ggcagtcggt ggcgctggcg ctggccgagc gccacaccgt gatcgcgccc
     1141 gacctgatcg gccacggcga ctccgagtcg ccgcgcgtgg actactcgct gggcgggcac
     1201 gccacacgca tccgtgacct gctggcctcg ctgggcatcg accgcgcgtc gatgatcggc
     1261 cactcctacg gcggcggcgt ggcgatgcag ttcttctggc agttccccca acgcaccgac
     1321 cggctgatgc tggtctcggc cggcggcctg ggcccggagg tcagcccgat gctgcgctcg
     1381 gccgcgctgc cgggggcgtc ggggctgctc tgggccatcg cccatccgcg cgtggtcgag
     1441 gcgctcacgg gcgcgggcac catcggccgc caggtctcgc gcgcgctgcg acccctgtcc
     1501 gggcccggtc cccggcaggc gttcctgcag acgctgcgct cggtgatcga cgtccgcggc
     1561 cagcgcgtca gcgcgcgcga ccggctctac ctgctcagcc acgcgcccac gcccacgcag
     1621 atcgtctggg gcgggcgcga ccacaccatc cccgtcgccc acggcgagtt cgcacaccgg
     1681 gcggtgccgc acagccgctt cgacgtcctg cccgaggcgg cccacttccc ccacctcgag
     1741 gaccccgagg ggctggcgcg ggtgctgggc gagttcctgg aggagaccga gcccgcgccg
     1801 gtggacgacg ccgactgggg cgacgtgatc gccgaaaggg ccccgcgctc acgcgtgggg
     1861 gcgtagtctc ggtaggcgat ggagtcccgg gaagccagcg gccaggggta tgtggaaggc
     1921 gtgtcctcgg gcgagcgcgg gccgatccgc gaggaggccg gctcgacgcc catgccccgg
     1981 cacccgggcg aggcgctgac cgagcccgcc gcggtcatcg agccgccctc gacgccggac
     2041 ggcacgttcg tgaaggtccg ggcgtccgac catcccctcc cgcgggcgtg cgaggtcacg
     2101 gtgaacttcc aggactacca ccgctccagc tccaagctgg gccgcttcgg caagatcgtg
     2161 ggcatcgacc tggacggggg cgagtacctg gtcatgctgg acaacttcga gtgcctggcg
     2221 gcgccgatgc ggcgcgtgga gtggcgcaag gtcgcgacgt aaccgctccg gtctccctgg
     2281 aggagctgga ggccgacccc cacgccacgc tggcccgcct gcgggcgacc gagccggtgg
     2341 cgtgggtcga cgcgctgaac ggctggctgg tgacctcgcg cgcgctggcg ctggaggtca
     2401 tgcgcgacgc gggccggttc acggtcgacg acccccgctt caccaccgcc cgcgtggtcg
     2461 gcccgagcat gctctcgctg gacggcgccg agcacgcccg tcaccgcgag ccctggctcc
     2521 cgcgcttcgg ccgcggccgc gtgcacgagc ggctggggcc ggtggtcacg cgcgagaccg
     2581 accgcctcct ggacgccgtc gccgaccgcg gccgtgccga cctgcgcgcc gccctggcgg
     2641 gcccgctggc cgccgcggtg gtcggccacg ccctggagct ggaggcggac ccgggaacgg
     2701 tgctgggctg gtaccggggg atcgtggact cggtgcagac gatgaccgcg ggcggcgagc
     2761 ccaccgccgc gggccgcgag gcgttcgccg agctggccgc agcgctggac accgacgccg
     2821 cgtcggacgc cgcggtgatc ctgttcggcg gcatcgagac caccgagggc atgatcgcca
     2881 cggcgctctg gtacctgctg cgcgagccgg gcggggtcga gcgggcgctg gccgagccgg
     2941 cgtggctgga gggcgccatc gaggaatcgc tgcggctgga gcccgccgcc gccatggtcg
     3001 accgctacgc cacggcggac accacgctcg gcaccgccgc ggtgcgcgcc ggggacctgg
     3061 tggcggtctc ggtcaccgcg gccaaccgcg acccggagtc cttccccgag cccgaccggt
     3121 tcgacccgct gcggtcgtac gcgaagcgcc acctggcgtt cgcctccggg ccgcacgtgt
     3181 gcatcggcgt gcacctggcc cgcctggagg cacacaccgc cgtgcggcgt gtgctggagc
     3241 gcctaccggc gctggcgctg gaccccgggc gctcggccgc gccgcgcggc ctggtcttcc
     3301 gcaagccgcc tcagctggag gtgacctggc ggcccgagcc cgagcgcagc tcgctgcgca
     3361 cgtagcgctc gcccgcccac gcgcggaggc ggtcccggcc gatcacgaac gcagggccgg
     3421 cggcgctgcc cagcagcgcc gtcagcagcg gggcgaacag catccctccc aggaagagca
     3481 ggccgaacgc ggcgccggcc tggccgccca gcagggcgac gatgaacagg cgggtctctt
     3541 ccgaacgctg gtgcatgcgc agtggttcac ccgcgcgcgg ggcctccctc ccgctgcaac
     3601 gtttcgtgcc cctgcttacg tagatcggca tgccgtcgga gcgcaccaag accttcacgc
     3661 tgatcgtgtg ctgcctggcc cagttcatgg tggtcctgga cgtgtcgatc
//
LOCUS       DASUPW010000476         2281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_2051396, whole genome shotgun sequence.
ACCESSION   DASUPW010000476 DASUPW010000000
VERSION     DASUPW010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2051396"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VFY44_10665"
     CDS             complement(<1..730)
                     /locus_tag="VFY44_10665"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEX5910948.1"
                     /translation="MAVSSVEQTVLPDGRVELDPAVDASLEGSSLHNEDLAPVPIEKR
                     TWTTYNYLALWVGMAINIPSWLLASGLIAIGMSWTQAIMTIFLGNLIVLIPMLLISHA
                     GTKYGIPYPVIARSSFGTIGANLPAITRGIIACFWFGIQTWIGGTALFAVVAALLGPD
                     SWWVNAQQFSIVAGDPQPWTMWLSFGIFWFLNMLIIVWGMEAIKKFENWAAPFLIVVF
                     LGLMVYMVVQAGGLGPTLAEPGTLG"
     gene            1056..>2281
                     /locus_tag="VFY44_10670"
     CDS             1056..>2281
                     /locus_tag="VFY44_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5910949.1"
                     /translation="MTATYRVPGAILTEREHRVPLDHAHPDGRSLTVFTREVADPDGM
                     DRPYLVFLQGGPGFEATRPTSPPSGWMKRALQDYRVLLVDQRGTGRSTPVGAVIPGET
                     AEDQAAYLALFRADSIVGDCEWIRGELEVDRWTVLGQSFGGFTALTYLSQAPQGLAAA
                     LITGGLAPVTGRPVDDVYGATWVRTIEANARYFARYPEDRARVAEMARRLEDEDVRLP
                     SGDRLTARRFRQLGMWLGDSAGFELIHHVLELPFGSTAFLADVDLPVRFARNPIYATL
                     HESSYADGGPTRWSAHRLAPDAYLAGDLLSAEHVFPWMWEDYGALRLHAGAAELLADH
                     DWPRLYDPTILAQNEVPVAATIYVDDVYVDRGFAEETAAQVRGLKPWITNEYAHNGLR
                     ADGERVVGRLLDLINGR"
BASE COUNT          334 a          799 c          835 g          313 t
ORIGIN      
        1 agccgagggt cccgggctcc gccagggtcg ggccaaggcc tccggcctgg accaccatgt
       61 agaccatgag gccgaggaag accacgatga ggaacggggc cgcccagttc tcgaacttct
      121 tgatcgcctc catgccccac acgatgatca gcatgttgag gaaccagaag atcccgaagc
      181 tgagccacat cgtccacggc tgcgggtcac ctgccacgat cgagaattgc tgggcgttca
      241 cccaccacga gtcggggccc aggagggccg caacgaccgc gaacagggcc gttccaccga
      301 tccacgtctg gatgccgaac cagaagcacg cgatgatccc gcgggtgatc gcggggaggt
      361 tggccccgat cgtcccgaac gacgaccggg cgatgaccgg ataggggatg ccgtacttgg
      421 tgccggcatg gctgatcagc agcatcggga tcagcacgat caggttgccc aggaagatcg
      481 tcatgatcgc ctgggtccag gacatcccga tggcgatcag gccggaggcc agcagccagc
      541 tcgggatgtt gatggccatc ccgacccaca gggccaggta gttgtaggtg gtccaggtcc
      601 gcttctcgat ggggaccggg gccaggtcct cgttgtggag tgacgagccc tccaggctcg
      661 cgtccacggc gggatcgagc tcgacccgtc cgtccggcag gaccgtctgc tccaccgatg
      721 agaccgccat gctcctcctc ctcctccggg gggccgcctc ggccccgcgt gctagctgcg
      781 cgccggccgc gccgactccc tccctgcgcg cccctccagc gcgccgccgc ggacacggcg
      841 cagggcggcc gcatgcacgg gtcggagacg gggaacgaga ggccgcggga tcgcgggttg
      901 agcgccgtgc gatcggtgcg gatgcaccat ccggtctcgg cctcctggcg acggatgtcg
      961 cgatcgatgg cccgcatgtt agccacttcg cgcccgcccg acgcggccgg cgcgctaggc
     1021 tggcacccca gccgcaatgg aggcgcccga tggccgtgac cgccacctat cgcgtccccg
     1081 gcgcgatcct gaccgagcgc gagcaccgcg tgccgctcga ccacgcgcac ccggacgggc
     1141 ggtcgctgac cgtgttcacc cgcgaggtcg cggacccgga cgggatggac cgcccgtacc
     1201 tcgtgttcct ccagggcggc cccgggttcg aggcgacccg gcccacgagc ccgccgtccg
     1261 gctggatgaa gcgcgcgctg caggactacc gcgtgctgct ggtggaccag cgcgggaccg
     1321 ggcggtcgac gccggtcggc gcggtgatcc cgggcgagac cgccgaggac caggccgcgt
     1381 acctggcgct gttccgggcg gactcgatcg tgggcgactg cgagtggatc cggggcgagc
     1441 tcgaggtgga ccgctggacg gtcctggggc agagcttcgg cgggttcacc gccctcacgt
     1501 acctgtcgca ggcaccccag gggctcgcgg cggcgctgat cacgggcggg ctggcgccgg
     1561 tgaccggccg gccggtggac gacgtctacg gcgcgacctg ggtgcggacg atcgaggcca
     1621 atgcgcgcta cttcgcccgg tacccggagg accgggcgcg ggtcgcggag atggcgcggc
     1681 gcctggagga cgaggacgtg cggctgccgt cgggcgaccg gctgacggca cgccggttcc
     1741 ggcagctggg gatgtggctg ggtgactcgg cggggttcga gctgatccac cacgtgctgg
     1801 agctgccgtt cgggtccacg gcgtttctcg ccgacgtgga cctgcccgtc cgcttcgcgc
     1861 gcaacccgat ctacgccacg ctccacgagt cgtcgtacgc cgacggcggc ccgacgcgct
     1921 ggtccgcgca ccggctggcg cccgacgcct acctcgccgg cgacctgctg agcgccgagc
     1981 acgtcttccc ctggatgtgg gaggactacg gggcgctgcg gctccacgcc ggcgcggcgg
     2041 agctgctggc cgaccatgac tggccgcggc tctacgaccc gaccatcctc gcccagaacg
     2101 aggtgccggt cgccgcgacg atctacgtcg acgacgtcta cgtggaccgg gggttcgcgg
     2161 aggagacggc cgcccaggtg cgcggcctga agccgtggat caccaacgag tacgcgcaca
     2221 acgggctgcg ggcggacggc gagcgggtgg tgggccggct gctcgacctg atcaacgggc
     2281 g
//
LOCUS       DASUPW010000477         2362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_389334, whole genome shotgun sequence.
ACCESSION   DASUPW010000477 DASUPW010000000
VERSION     DASUPW010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389334"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..1730)
                     /locus_tag="VFY44_10675"
     CDS             complement(189..1730)
                     /locus_tag="VFY44_10675"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEX5910950.1"
                     /translation="MAAAAVACVAAAPAAAAPSGSQKLQDALTVKNIVNHERQLAAIA
                     GRNGNTRASGTPGYDKSVEYVSKKLTKAGYKVTKQTFLFPYFEETAPGSFARVSPQPK
                     TYTEDEFSVMQYSGSGTAEAKVVPVDVTVPPTPEPSSTSGCEPADFAGFTAGSIALVQ
                     RGTCDFGVKAQNAKAAGASAVVIFNEGQPGRTDGEGGTLGAPIDIPVVFSSYAAGEEL
                     VNLAKAGDVTVKITTSTISETRPTYNVIADSPAGRKNRTVVVGAHLDSVVEGPGINDN
                     GSGTSTNLEIALQMAKKGIEPRNRVRFAFWGAEESGLLGSEDYVGKLSDAERDKIAAN
                     LNFDMLGSPNYARLVYDGDGSEDPETGPGPVGSDTIERIFNRHFASKGLATEPTAFDG
                     RSDYGPFIDVGIPAGGLFSGAEELKTAEQVAKFGGTAGLALDPCYHQACDNIDNLNKQ
                     ALDELGDGAAHAVYTLAERKKSIVVLKKAKTAKAAKARTAKASRASGKKLARSFDYRG
                     SHLFR"
     gene            complement(1880..>2362)
                     /locus_tag="VFY44_10680"
     CDS             complement(1880..>2362)
                     /locus_tag="VFY44_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910951.1"
                     /translation="NAATGRGCLARRSPVGPRNIGRIRLGMTRKTLAKRVPEPVRKTK
                     RSWRWCVKGGKGTVSAAFTRKGKVALVSTTAPRHGNRGVLVGSRTKTVRAKFARRRAV
                     SKTLFRANKRSPRLFGVRKGKVRYWAVTSRKTIGRKRDLRAYLRYAGVAAKAKPKRKR
                     "
BASE COUNT          290 a          850 c          818 g          404 t
ORIGIN      
        1 acgaacgcca ccacggccgc cagcaccgcc ttcggccgct tccaggtcag gtcggcgacg
       61 cgctcgagca tgagggtgcg ggacggtacc agagtgagtt tccttaggta actcaccctc
      121 ccggcccgac gcaggagggg cggcgctctc gcgccgcccc tcgatgctgc gtcgggtgct
      181 gcctgcggct aacggaacag gtggctgccc cggtagtcga agctgcgcgc cagcttcttg
      241 ccggaggccc gcgaggcctt cgcggtgcgc gccttggccg ccttggcggt cttggccttc
      301 ttcagcacca cgatcgactt cttgcgctcg gccagcgtgt acaccgcgtg cgcggcacca
      361 tcgccaagct cgtcaagcgc ctgcttgttg aggttgtcga tgttgtcgca cgcctggtgg
      421 tagcacgggt ccagcgccag cccggcggtg ccgccgaact tggccacctg ctcggccgtc
      481 ttcagctcct ccgcaccgct gaacaggccg ccggccggaa tgccgacgtc gatgaacggg
      541 ccgtagtcgg accgtccgtc gaacgccgtc ggctcggtgg ccaggccctt ggacgcgaag
      601 tggcggttga agatccgctc gatcgtgtcc gagcccacgg ggccgggacc ggtctccgga
      661 tcctcggagc cgtcaccgtc gtagaccagg cgggcgtagt tgggcgaccc gagcatgtcg
      721 aagttcaggt tcgccgcgat cttgtcccgc tcggcgtcgc tgagcttgcc gacgtagtcc
      781 tcggagccca gcaggccgga ctcctcggcg ccccagaagg cgaacctgac gcggttgcgc
      841 ggctcgatgc ccttcttggc catctgcagg gcgatctcca ggttggtgga ggtgccgctg
      901 ccgttgtcgt tgatgcccgg gccctcgacg accgagtcca ggtgcgcacc cacgacgacc
      961 gtccggttct tgcgcccggc cggcgagtcg gcgatcacgt tgtacgtggg ccgcgtctcg
     1021 ctgatggtcg acgtcgtgat cttcacggtc acgtcaccgg ccttggccag gttgaccagc
     1081 tcctcgcccg cggcgtagct gctgaagacc accgggatgt cgatcggcgc gcccagcgtg
     1141 ccgccctccc cgtcggtccg gcccggctgg ccctcgttga agatgaccac cgcggatgcg
     1201 ccggccgcct tcgcgttctg ggccttgacg ccgaagtcgc acgtgccgcg ctggaccagc
     1261 gcgatcgacc cggccgtgaa gccggcgaag tcggcgggct cgcagccgct ggtgctggag
     1321 ggctccggcg tgggcggcac ggtcacgtcc accgggacca ccttggcctc ggccgtgccg
     1381 ctgcccgagt actgcatgac gctgaactcg tcctcggtgt aggtcttcgg ctgcggcgac
     1441 acgcgcgcga acgagcccgg cgcggtctcc tcgaagtagg ggaacaggaa cgtctgcttg
     1501 gtgaccttgt agcccgcctt ggtgagcttc ttgctcacgt actccacgga cttgtcgtac
     1561 cccggggtgc ccgaggcgcg ggtgttgccg ttgcgcccgg cgatggccgc cagctggcgc
     1621 tcgtggttga cgatgttctt gaccgtcagg gcgtcctgca gcttctggct gccgctgggc
     1681 gccgctgccg ccggcgccgc tgccacacag gccacggcgg ccgcggccaa caggcctact
     1741 gctcgcttgc gatgcacgaa tcgacctcct ggtcgacgag tttgtgggcg ccggcttccc
     1801 gacgaagccg cgccccggat ctgcggaacc cctccccgct ggcccggagg cccaaacgcg
     1861 ccgagagcgg tcgcggcggc taacgcttgc gcttgggctt ggccttggcg gccacgcccg
     1921 cgtaccgcag gtaggcgcgc agatcgcgct tgcgcccgat cgtcttccgc gaggtcacgg
     1981 cccagtagcg gaccttgccc ttgcgcacgc cgaacaggcg cgggctgcgc ttgttggcgc
     2041 ggaacagcgt cttgctgacc gcgcggcggc gggcgaactt cgcgcgcacc gtcttcgtcc
     2101 tgctgcccac cagcacgccg cggttgccgt gccgcggggc ggtggtgctg accagcgcca
     2161 ccttgccctt gcgggtgaac gcggcggaga ccgtgccctt gccgcccttc acgcaccagc
     2221 gccaggagcg cttggtcttg cgcaccggct cgggcacccg cttggccagc gtcttccggg
     2281 tcatgcccag gcgaatccgg ccgatgttcc tgggccccac cggcgagcgc cgcgccaggc
     2341 agccgcggcc ggtggccgcg tt
//
LOCUS       DASUPW010000478         3925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_803815, whole genome shotgun sequence.
ACCESSION   DASUPW010000478 DASUPW010000000
VERSION     DASUPW010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803815"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1123)
                     /locus_tag="VFY44_10685"
     CDS             complement(128..1123)
                     /locus_tag="VFY44_10685"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HEX5910952.1"
                     /translation="MPSAAPATTVTPAERAVRTAGIASVAAAVPREEVASATVAARLG
                     VDEQWIERRTGVRSRHVAPAAVRLDELAAEAAEQALEQAGTRAADLDLVLVATTTADE
                     LLPNAAPLVADRIGATRAGAMDLGAACTGFLSALGQAAAQVEAGRADAVLVVGADLMA
                     RVTDPHDRATAALFGDGAGAAVVTADGPGAIGPVVLGADAANGRDLIRIEHSDRLVQM
                     AGHETFRNAVARLSQSSRLAAERAGVTLDEIDLFVYHQANTRILDAVGRELGLAPERV
                     VDCIASYGNTSAATIPIALRVAADAGRLEPGARVLLGAFGAGFTWGAALVEWGAA"
     gene            complement(1116..1943)
                     /locus_tag="VFY44_10690"
     CDS             complement(1116..1943)
                     /locus_tag="VFY44_10690"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX5910953.1"
                     /translation="MSASNTPTAILFPGQGSQQPGMREPARAVLPELVELADELCDGD
                     PFARADQGTRFQQPALYCAALAAWEGLRELEPVALAGHSLGEITALAAAGALTPEDGL
                     RLVTVRGALMQQAADAGDGGMMAVRGARADVEPLVRDTAVAVANDNSPRQVVLAGPAA
                     DLDALGDVRGKRLPVAGAFHSPLMEPAVEGFRAELERTEIREPALPVISCVTAEPFTD
                     VREQLAAALTSPVRWVDVLGALRGRGVRRFVETGPGSVLTGLVRKTLDDAEAVAAHA"
     gene            2062..2481
                     /locus_tag="VFY44_10695"
     CDS             2062..2481
                     /locus_tag="VFY44_10695"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX5910954.1"
                     /translation="MPSEPFIHHERVRFGDLDAMRHLNNVVFLRYFETARISYMRRLT
                     PDHDPADPEAGGFGFIFAECHINYRSPVYFDEEVEVRCSIGEVRRSAFQVAFEMTVGD
                     RVAAEGYGWLVGFDYTAQESARLPDALRERLAAEAAA"
     gene            complement(2478..3077)
                     /locus_tag="VFY44_10700"
     CDS             complement(2478..3077)
                     /locus_tag="VFY44_10700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5910955.1"
                     /translation="MPERLRLLAVDDHEVVRWGFRLLMADQPWVERCLTAGTPDEALD
                     LAARYRPHVAVIDLFMGDSSGAELCSTLREREPATKVLLISGAGWLSPAAARAAGASG
                     FVPKDWDAADVTSAVRMVANGMTVFVPREERPASSLSSRERQIVELMAQGSTNGEIAG
                     ELHLSPHTVKDHASAVYRKLGVRNRAEAVRRAERLGLTA"
     gene            complement(3146..3586)
                     /locus_tag="VFY44_10705"
     CDS             complement(3146..3586)
                     /locus_tag="VFY44_10705"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5910956.1"
                     /translation="MERPLALVAVLIVGGLIALQPPVNSQLARETSILGAAFISTAIS
                     ALVMGVIFFAAGGASQLSAVPSVPPIYLTGGLLGAVLVTVSLVAVRTLGAGGVVAATV
                     TTQLIVSAVLDRLGVLGLDEIGLTPLRVAGFALLLTGTLMVTIR"
     gene            complement(3593..>3925)
                     /locus_tag="VFY44_10710"
     CDS             complement(3593..>3925)
                     /locus_tag="VFY44_10710"
                     /inference="COORDINATES: protein motif:HMM:NF024672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEX5910957.1"
                     /translation="DVRVLRQADPAGAAGGTAREHHLRGVRVSEPRDVPILGRMIRLG
                     QLLKLAGAIDSGSDAKHLLATETVLINGEPDVRRGRQLHHGDLVTVGGEELRVVVPGQ
                     DEEAAPAK"
BASE COUNT          496 a         1487 c         1478 g          464 t
ORIGIN      
        1 gtctcgtcgg cgccgtcctg gtcggagcgg tagttcacgg ccacgggcca gccggcgtcg
       61 gccagcgcgc gggcgctggc ggcgccgatg ccgcgggagg cgccggtgac cagggcgcag
      121 ccctccatca ggcggccccc cattcgacca gcgccgcgcc ccaggtgaag ccggcgccga
      181 acgcgcccag caggacgcgc gcaccgggct caaggcggcc ggcgtcggcg gccacccgca
      241 acgcgatggg aattgtggcc gccgacgtgt tgccgtagct ggcgatgcag tccacgaccc
      301 gctcgggcgc caggcccagc tcgcggccca ccgcatccag gatgcgggtg ttggcctggt
      361 ggtagacgaa cagatcgatc tcgtccagcg tcacgccggc gcgctcggcg gccaggcggc
      421 tggactggct caggcgggcg accgcgttgc ggaaggtctc gtgcccggcc atctggacga
      481 gccggtcgga gtgctcgatc cggatcaggt cgcgcccgtt ggcggcgtcg gcgcccagca
      541 cgaccgggcc gatggccccg gggccgtccg cggtgaccac ggcggcgccg gccccgtcgc
      601 cgaacagcgc ggcggtggcg cggtcgtgcg ggtcggtcac gcgcgccatc aggtcggcgc
      661 ccaccaccag gacggcgtcg gcgcggccgg cctcgacctg tgccgcggcc tggcccagcg
      721 ccgacaggaa tccggtgcac gccgcgccca ggtccatggc gccggcgcgg gtggcgccga
      781 tgcgatcggc caccagcggc gcggcgttgg gcagcagctc gtcggcggtc gtggtggcca
      841 ccagcaccag gtccaggtcc gccgcgcggg tcccggcctg ctccagcgcc tgctcggcgg
      901 cctcggcggc gagctcgtcc aggcgcacgg cggcgggggc cacgtgccgc gagcgcacgc
      961 cggtgcggcg ctcgatccac tgttcgtcca cgcccaggcg ggcggcgacg gtggcgctgg
     1021 ccacctcctc gcgcggcacg gcggcggcca cggaggcgat gcccgcggtg cggacggcgc
     1081 gctcggccgg ggtgaccgtg gtggcgggag cggcgctagg catgcgcggc caccgcttcg
     1141 gcgtcgtcca gggtcttgcg caccaggccg gtcagcaccg agccggggcc ggtctcgacg
     1201 aaccggcgca cgccgcggcc ccggagcgcg cccagcacgt cgacccagcg caccggcgac
     1261 gtcagcgccg cggccaactg ctcgcgcacg tcggtgaacg gctcggccgt gacacaggag
     1321 atcaccggaa gggcgggctc gcggatctcg gtccgctcca gctcggcgcg gaagccctcg
     1381 accgcgggct ccatcaaggg cgagtggaac gcgccggcca ccggcaggcg cttgccgcgc
     1441 acgtcgccga gcgcgtccag gtcggctgcg ggcccggcca ggaccacctg gcgcggcgag
     1501 ttgtcgttgg ccacggcgac ggccgtgtcg cgcaccagcg gctcgacgtc cgcgcgggcg
     1561 ccgcggacgg ccatcatgcc gccgtcgccg gcgtcggccg cctgctgcat cagggcgccg
     1621 cgcacggtca ccagccgcag cccgtcctcg ggcgtcaggg cgccggcggc ggccagggcg
     1681 gtgatctcac cgagggagtg gcccgccagg gcgacgggct cgagctcgcg caggccctcc
     1741 cacgcggcca gcgccgcgca gtacagggcg ggctgctgga agcgggtgcc ctggtcggcg
     1801 cgggcgaacg gatcgccgtc gcagagctcg tcggccagct cgaccagctc gggcagcacg
     1861 gcgcgcgcgg gctcgcgcat gccgggctgc tggctgccct ggccggggaa gaggatcgcg
     1921 gtgggtgtgt tcgaagcgct catggcgtac cgaacccttc accgccggcc gcccggcgca
     1981 acggcccgcg accgccatac cccccggccg ggggatcggg cggcgagcag tggggggtgc
     2041 ggcggggcta acgtggccgc cgtgccctcc gagcccttca tccaccacga gcgcgtgcgc
     2101 ttcggcgacc tggacgccat gcgccacctg aacaacgtcg tgttcctgcg ctacttcgag
     2161 acggcgcgga tcagctacat gcgccggctg acgccggacc acgaccccgc ggacccggag
     2221 gccggcggct tcggcttcat cttcgccgag tgccacatca actaccgctc gcccgtctac
     2281 ttcgacgagg aggtcgaggt ccgctgcagc atcggcgagg tgcgccgctc ggcgttccag
     2341 gtggccttcg agatgacggt gggcgaccgc gtggccgccg agggctacgg ctggctggtg
     2401 ggcttcgact acaccgccca ggagtcggcg cggctgcccg acgccctgcg cgagcggctc
     2461 gcggccgagg ccgcggccta ggccgtcagc cccagccgct ccgcgcgccg cacggcctcg
     2521 gcccggttgc gcacgccgag cttgcggtag acggcgctgg cgtggtcctt gacggtgtgg
     2581 ggcgacaggt gcagctcgcc cgcgatctcg ccgttggtcg agccctgggc catcagctcc
     2641 acgatctggc gctcacgcga ggacaggctc gaggcggggc gctcctcgcg cggcacgaac
     2701 accgtcatgc cgttggccac catgcgcacg gcgctggtca cgtcggcggc gtcccagtcc
     2761 ttggggacga agcccgacgc gccggcggcc cgcgcagccg cgggcgacag ccagccggcg
     2821 ccggagatca gcagcacctt ggtcgcgggc tcgcgctcgc gcagcgtgga gcacagctcg
     2881 gcgcccgagc tgtcccccat gaacaggtcg atgaccgcca cgtgcgggcg gtagcgcgcg
     2941 gccaggtcca gcgcctcgtc cggggtgccg gcggtcaggc agcgctcgac ccagggctgg
     3001 tcggccatca gcaggcggaa cccccagcgc accacctcgt ggtcgtcgac ggccagcagt
     3061 cgcaggcgct cgggcatccc cgcccgtatc cccgccggtc ttggcgcgtt agcgccacgg
     3121 gggtcaagac cccccgagtg gggggtcagc ggatggtgac catcagcgtg ccggtcagca
     3181 gcagcgcgaa gccggccacg cgcagcggcg tcagcccgat ctcgtccagc cccagcaccc
     3241 ccaggcggtc caacaccgcc gacacgatca gctgcgtggt caccgtcgcg gccaccacgc
     3301 cgcccgcgcc cagcgtgcgc acggcgacca gcgacaccgt caccagcacc gcgcccagca
     3361 ggccgcccgt caggtagatc ggcggcaccg agggcaccgc cgacagctgc gaggcgccgc
     3421 ccgcggcgaa gaagatgacg cccatgacca gcgccgagat ggcggtgctg atgaacgcgg
     3481 cgcccaggat gctggtctcg cgcgccagct gcgagttgac cggcggctgg agggcgatca
     3541 gccctccgac gatgagcacg gccaccaggg ccagagggcg ctccatgcgc ccctacttcg
     3601 ctggcgcggc ctcctcgtcc tgcccgggca ccacgacacg cagctcctcg ccgccgacgg
     3661 tgaccaggtc gccgtggtgc agctggcggc cgcggcggac gtcgggctcg ccgttgatca
     3721 gcacggtctc ggtggccagc aggtgcttgg catcgctgcc gctgtcgatc gccccggcca
     3781 gcttgagcag ctggcccagc cggatcatgc ggcccaggat cgggacgtcg cgcggctcgc
     3841 tcacgcgcac tccacgcagg tggtgctctc gggccgtgcc tccagccgcg ccggcgggat
     3901 cggcttgccg caggactcgc acgtc
//
LOCUS       DASUPW010000479         5346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_942063, whole genome shotgun sequence.
ACCESSION   DASUPW010000479 DASUPW010000000
VERSION     DASUPW010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942063"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1617)
                     /locus_tag="VFY44_10715"
     CDS             complement(64..1617)
                     /locus_tag="VFY44_10715"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5910958.1"
                     /translation="MLFAVVFAIREAFGNPTDGLTFLYLLPICLVAIERGVRAGLAAA
                     GVGLALVAVWDHMDAQGLTTLAYVTRAVVFGGVGMLCGTMAARVRKAGAESERYFEMS
                     SQLLGIFTLDGRPRRMNRRWSELLGRPMDELMAMGLADVVHPDDLPLAQRHLSEILNA
                     DRGGQISHTVRMLTATGGVRHVNVHNTLDAEEGLLYMSAEDVTERHELESARQQAEER
                     FRVAFENSATGMAVVALTEEHSGQIVEANEELGRVLGKPREQLLGMSGLVDFAHPEDM
                     AGLREDMAALATGEQEVVRSEIRILRPDGSLHWVHLTSSLLHGPDGEPLFRLSQIMDI
                     DARKQAEQQMQHMADHDPLSWLFNRRRFMDELGGELAGSRLRRTSGAVLLIDLDKFKQ
                     VNDLSGHAVGDEVIRTVSLALIRRLRSGDVAGRLGGDEFGVLLRRVTGEEAVLVSHDL
                     REAVAEALAMMDDEVARRVTLSIGIALVEGPRPGAGVLVERADAAMYEAKRDGGDRVA
                     LWSPVFAGA"
     gene            1735..2163
                     /locus_tag="VFY44_10720"
     CDS             1735..2163
                     /locus_tag="VFY44_10720"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5910959.1"
                     /translation="MRAYRVHVSHDFAKPPERVFAYMAEHENLGPLFGAEITRLRDGD
                     TDRNGVNSVRKLKVGPLPPFEETVHEFVPNERILYRITKGGPLKDHVGVLEFSPTPGG
                     GTHLEYNIRIASAIPGLAPIVASGLTRDIGKNLPMVDERA"
     gene            2213..3730
                     /locus_tag="VFY44_10725"
     CDS             2213..3730
                     /locus_tag="VFY44_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910960.1"
                     /translation="MRAALLTIVVALLVAPAVAHGAYAPLDRSGPPLEVSAAAVDAAT
                     KCSPGIAGATRAPVLLSPGTGATADENFSWNYQRSLTARGIPWCGITMPGHAQGDIQV
                     AGEYLVHAIRRIHRLSGRRVAVMGHSQGGMSMRWALRFWPDTRRMVDDVIGFAGSNHG
                     TETGGGCANGCTPAGWQQGARANFIVALNSGAETHAGVSYTEVYTHRDEVVTPNADDN
                     GSSSVRGGRGRIANVATQDICPANTSEHFIVGTTDPVAYALAMDALDHDGPASEARVD
                     RAACTQVFAPGVDPAYLDTYLQIVSVVPGVAADVAPVNTIGVPVVRQEPALRCYVFAE
                     CTPASGGSDAAGRCVSAVASARGTVFGPAALGRTRAAQRRRLPGRRGKNRKGIDRFCV
                     AGGGALEIGYPTSRLSRKGRRAARGRAILVLGSSPRFRVRGLAHGSRTATLRKRLRGE
                     RSYRVGRNRWYTARGAKATLVFRTRGGSVRAVGLASRRLPAGRAATRRLLRAWQL"
     gene            3740..4792
                     /locus_tag="VFY44_10730"
     CDS             3740..4792
                     /locus_tag="VFY44_10730"
                     /inference="COORDINATES: protein motif:HMM:NF016999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEX5910961.1"
                     /translation="MRKLGEWSLLGAATLALGAGLGAVGLPSSYLFGALLAGLGAALL
                     APGRLEVPDRAFLAAQAVTGVTLGAYLEPDSLEAVAGSWLPVLLVSAGTLALSVAAGK
                     LLARTTDCDETTASLGLVAGGATGIVAMADELGGDGRLVAFMQYARVLVVVLITPLVA
                     ALAFPGHASAPAAAMDTPLLGEPSWWAITAAVAVAGAGAGALVRLPNPWILGPLLVSG
                     VVTVVAPSGTFEVPELLREAAFIGIGAQVGLRFTPDTLREVGRLLWPVMVSIAGLIAG
                     CFGLAVLLHLTADVSLADAYLATTPGGFYAVAAIAFGANGDTTFIVAVQGLRVLVMVL
                     LAPALVRWLARSRTPA"
     gene            4802..>5346
                     /locus_tag="VFY44_10735"
     CDS             4802..>5346
                     /locus_tag="VFY44_10735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HEX5910962.1"
                     /translation="MASAALHYAAFTATPAGGNPAGVVLDATGMGDDQMLALAARLAY
                     SESAFLWPRAEDGHYDVRYFSPEAEVPFCGHATIASGVALAEHAGATGRLVLHTASGE
                     VPVDTETDGQGRTVATLTSVAPDVQEPDPALVTGALEHLRWDPGGLDPELPPLVAYAG
                     ARHLIVACRTRERLADVDYDF"
BASE COUNT          657 a         2039 c         1949 g          701 t
ORIGIN      
        1 ccgcaccggc ccggccgggg tggcgtgccg caacggcgcc ctgaaggtga ccgccgacgc
       61 gccctaggcg ccggcgaaga cgggcgacca gagcgcgacg cggtcgccgc cgtcgcgctt
      121 ggcctcgtac atggccgcgt cggcgcgctc gaccagcacg cccgcgccgg ggcgtgggcc
      181 ctcgaccagc gcgatgccga tgctcagcgt cacgcggcgc gcgacctcgt cgtccatcat
      241 cgcgagcgcc tcagccaccg cctcgcgcag gtcgtgggag accaggaccg cctcctcgcc
      301 ggtgaccctc cgaagcagga cgccgaactc gtcgccgccc agccggcccg cgacgtcgcc
      361 gctgcgcagg cggcggatca gcgccagcga gaccgtcctg atgacctcgt cgcccaccgc
      421 gtggccggac aggtcgttga cctgcttgaa cttgtccagg tcgatcagca gcaccgcgcc
      481 gctggtccgg cgcagcctgc tgccggccag ctcgccgccc agctcgtcca tgaaccggcg
      541 gcggttgaac agccagctca gcggatcgtg gtcggccatg tgctgcatct gctgctcggc
      601 ctgcttgcgg gcgtcgatgt ccatgatctg cgacaggcgg aacaacggct cgccgtcggg
      661 gccgtgcagc agggacgagg tcaggtgcac ccagtgcagg ctgccgtccg ggcgcaggat
      721 ccggatctcg ctgcgcacga cctcctgctc gcccgtggcc agcgccgcca tgtcctcccg
      781 tagccccgcc atgtcctcgg ggtgggcgaa gtccaccagc ccgctcatgc ccaggagctg
      841 ctcgcggggc ttgcccagga cccgcccgag ctcctcgttg gcctcgacga tctggccgct
      901 gtgctcctcg gtcagcgcga ccaccgccat ccccgtggcc gagttctcga acgcgacccg
      961 gaagcgctcc tccgcctgct ggcgcgcgct ctccagctcg tggcgctcgg tcacgtcctc
     1021 cgccgacatg tacaggaggc cctcctcggc gtcgagggtg ttgtgcacgt tcacgtgccg
     1081 cacgccgccc gtggccgtga gcatccgcac ggtgtgcgag atctggcccc cgcgatcggc
     1141 gttcaggatc tcgctcagat gccgctgggc cagcgggagg tcgtcggggt ggaccacgtc
     1201 ggcgaggccc atcgccatca gctcgtccat ggggcggccc agcagctcgg accagcgccg
     1261 gttcatccgc cgcgggcgcc cgtccagggt gaagatgccg agcagctggc tggacatctc
     1321 gaagtagcgc tcgctctccg cgccggcctt gcgcacgcgc gccgccatcg tgccgcacag
     1381 catcccgacc cctccgaaca ccacggcacg ggtcacgtag gccagcgtgg tcagcccctg
     1441 cgcgtccatg tggtcccaca cggccaccag cgccaggccg acgccggcgg cggccaggcc
     1501 ggcgcggacg ccgcgctcga tggccaccag gcagatcggc agcaggtaga ggaacgtcag
     1561 gccgtccgtc gggttgccga acgcctcacg gatggcgaac accacggcga agagcaccgc
     1621 ggcggccaga agtaccgcgc cccgcccccg aagcgcgcgc gcaggatcca aggcgtgcat
     1681 gtcccgtacc ccatcgggcg aacggccccc gcacttgagc catagggtgc gcccatgcgc
     1741 gcctatcgag tccacgtgag ccacgacttc gccaagcctc cggaacgggt gttcgcctac
     1801 atggccgagc acgagaacct ggggccgctg ttcggcgccg agatcacgcg cctgcgtgac
     1861 ggcgacaccg accgcaacgg cgtgaactcg gtgcgcaagc tgaaggtcgg gcccctccct
     1921 cccttcgagg agaccgtgca cgagttcgtg cccaacgagc ggatcctgta ccggatcacc
     1981 aagggcgggc cgctgaagga ccacgtgggc gtgctggagt tcagccccac ccccggcggc
     2041 gggacgcacc tggagtacaa catccggatc gcgtccgcca tcccgggcct ggcgccgatc
     2101 gtcgcgtcgg ggctgacgcg cgacatcggc aagaacctgc cgatggtgga cgagcgcgcc
     2161 taggccgacc cggcgcggcg ccgccgccgg cctgggagga tcggccgtcg cgatgcgcgc
     2221 cgccctcctc accatcgtcg tggccctcct ggtcgcgcct gccgtggcgc acggcgccta
     2281 cgcgccgctg gaccgcagcg ggccgccgct ggaggtgtcg gccgcggccg tggacgcggc
     2341 cacgaagtgc tcgccgggca tcgccggcgc cacccgtgcc ccggtgctgc tgtcccccgg
     2401 caccggcgcg accgcggacg agaacttcag ctggaactac cagcgctcgc tgaccgcgcg
     2461 cggcatcccg tggtgcggga tcacgatgcc cggccacgcg cagggcgaca tccaggtggc
     2521 gggcgagtac ctcgtgcacg cgatccggcg catccaccgc ctctcgggcc gccgggtggc
     2581 cgtcatgggc cacagccagg gcggcatgtc catgcgctgg gcgctgcgct tctggcccga
     2641 cacccgccgg atggtggacg acgtgatcgg cttcgccggc agcaaccacg gcaccgagac
     2701 cggcggcggc tgcgccaacg gctgcacccc ggccgggtgg cagcagggcg cgcgggccaa
     2761 cttcatcgtc gccctcaaca gcggagccga gacccacgcc ggggtgtcct acaccgaggt
     2821 ctacacgcac cgcgacgagg tcgtgacgcc gaacgccgac gacaacggca gctcctcggt
     2881 ccgcggtggg cgggggcgga tcgccaacgt ggccacgcag gacatctgtc ccgccaacac
     2941 cagcgagcac ttcatcgtcg gcacgaccga cccggtggcc tacgcgctgg ccatggacgc
     3001 gctcgaccac gacggcccgg cgtccgaggc ccgggtcgac cgcgcggcgt gcacccaggt
     3061 cttcgcgccc ggcgtggacc ccgcctacct ggacacctac ctccagatcg tctcggtggt
     3121 cccgggagtg gccgccgacg tggcaccggt gaacacgatc ggcgtcccgg tggtgcgaca
     3181 ggagcccgcg ctgcgctgtt acgtgttcgc cgagtgcacg cccgccagcg gaggctcgga
     3241 cgccgccggc cgctgcgtca gcgcggtcgc ctcggcccga ggcacggtgt tcgggccggc
     3301 ggcgctgggc cggacgcgcg cggcgcagcg acggcgtctg ccggggcgcc ggggcaagaa
     3361 ccgcaagggc atcgaccgct tctgcgtggc cggcggcggc gcgctggaga tcggctaccc
     3421 gaccagccgg ctctcgcgca agggccgccg cgccgcgcgc gggcgcgcga tcctggtgct
     3481 gggctcgagc ccgcgcttcc gcgtgcgggg gctggcccac ggttcgcgca cggccaccct
     3541 gcgcaagcgc ctgcgcggcg agcgcagcta ccgcgtgggc cgcaaccgct ggtacacggc
     3601 gcgcggcgcc aaggccaccc tggtgttccg cacacgcggc gggtccgtgc gcgcggtcgg
     3661 gctggccagc cgccggctgc ccgccggccg ggccgccacg cggcggctgc tgcgcgcctg
     3721 gcagctctag attcaggcgg tgcgcaagct gggcgaatgg tcgctgctgg gcgcggccac
     3781 gctggccctg ggcgccggcc tgggcgcggt gggcctgccc tcgtcgtacc tgttcggggc
     3841 gctgctggcc gggctgggcg cggcgctgct ggcgcccggc cggctggagg tccccgaccg
     3901 ggcgttcctg gcggcgcagg cggtcaccgg ggtcaccctg ggcgcatacc tcgagcccga
     3961 ctcgctggag gccgtcgccg gctcgtggct tccggtgctg ctggtcagcg ccggcaccct
     4021 ggccctgagc gtggccgccg gcaagctgct ggcgcgcacg accgactgcg acgagaccac
     4081 cgcgtcgctg gggctggtgg ccggcggcgc caccggcatc gtggcgatgg ccgacgagct
     4141 cggcggcgac ggccggctgg tggcgttcat gcagtacgcg cgcgtgctgg tcgtggtgct
     4201 gatcaccccg ctcgtggccg cgctggcctt ccccggccac gccagcgcgc cggcggcggc
     4261 catggacacc cccctgctgg gcgagccctc gtggtgggcc atcaccgccg cggtcgccgt
     4321 cgcgggcgcc ggggccggcg cgctggtgcg cctgcccaac ccgtggatcc tgggcccgct
     4381 gctggtgtcg ggcgtggtca cggtggtggc gccctcgggc acgttcgagg tgcccgagct
     4441 gctgcgcgag gcggccttca tcggcatcgg cgcccaggtg ggcctgcgct tcacgcccga
     4501 cacgctgcgc gaggtcgggc gcctgctgtg gccggtgatg gtgagcatcg cggggctgat
     4561 cgcgggctgc ttcggcctgg ccgtgctgct gcacctgacc gccgacgtct cgctggccga
     4621 cgcctacctg gccaccacac cgggcggctt ctacgccgtc gccgcgatcg ccttcggcgc
     4681 caacggggac accacgttca tcgtggccgt gcagggcctg cgcgtgctgg tcatggtgct
     4741 gctggcgccg gcgctggtgc ggtggctggc gcgatcgcgc accccggcgt agcctggcgc
     4801 ggtggcctcc gccgcactgc actacgcggc gttcaccgcc accccggcgg gcggcaaccc
     4861 ggccggcgtg gtgctggacg cgacgggcat gggcgacgac cagatgctgg ccctggcggc
     4921 gcggctggcg tactcggagt ccgccttcct gtggccccgg gccgaggacg gccactacga
     4981 cgtccgctac ttcagccccg aggccgaggt gcccttctgc gggcacgcca cgatcgcctc
     5041 gggcgtggcg ctggccgagc acgccggggc gaccggccgg ctggtgctgc acaccgccag
     5101 cggcgaggtc ccggtggaca cggagaccga cggccagggc cgcaccgtgg ccacgctgac
     5161 cagcgtcgcg cccgacgtgc aggagcccga cccggcgctg gtgaccggcg ccctggagca
     5221 cctgcgctgg gacccgggcg gcctggaccc cgagctgccc ccgctggtgg cgtacgcggg
     5281 cgctcgccac ctgatcgtcg cctgccgcac gcgcgagcgc ctggccgacg tggactacga
     5341 cttcga
//
LOCUS       DASUPW010000480         2493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1773758, whole genome shotgun sequence.
ACCESSION   DASUPW010000480 DASUPW010000000
VERSION     DASUPW010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1773758"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..1072
                     /locus_tag="VFY44_10740"
     CDS             95..1072
                     /locus_tag="VFY44_10740"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5910963.1"
                     /translation="MIDQRPLGTQGLTASAQGLGCMGMSEFYGDGDDTESVATIHRAI
                     ELGVNFLDTAAMYGSGANEQLVGKAIADRRDEVVLATKFGIVRGEDPRERGVNGRPEY
                     VKQSCDDSLRRLGLDHVDLFYQHRVDPETPIEETVGAMGELVDAGKVRFLGLSEAAPE
                     TIRRAHATHPISALQTEYSLWTRHVEDELLETVRELGIGFVAYSPLGRGFLTGRFKSA
                     DALDENDFRRHNDRFSGDNLDANVALVEKVEALAAAKEVTPGQLALAWVHAQGDDVFP
                     IPGTKRRAYLEENAAAFEVELTADDLAALDEAVPAGAAAGDRYPADPER"
     gene            complement(1069..>2493)
                     /locus_tag="VFY44_10745"
     CDS             complement(1069..>2493)
                     /locus_tag="VFY44_10745"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5910964.1"
                     /translation="NIAGATVVLFLALYVVPRPDIGDESGALVANFIAFGVFLGVAIA
                     VGVVWGHRRLSPMRAWLRADRAPDADEQRLTLRAPRRILLVNSFLWTLAVVVFTVVNF
                     AFSPGLGLVVAIVVALGGMTTSAVAYLISERLLRPVVARALSQRPPDRQVTAGIVTRA
                     LAAWALGTGVPVVGLVLVAIASLAGREVTETQLAITAVCLGGVSLTVGLLTAWQASRG
                     TADPVLSVREALNRVEDGDFEAEVPVFDGSELGLLQSGFNRMAAGLREREEIRELFGR
                     QVGTDVAKAALEQGTEMGGETREVAVLFTDIVGSTSLAQDRPPHEVVDMLNKFFAVVV
                     DVVEEHGGWINKFEGDAALAVFGAPIPLDDAADRALAAARSLAARLAEEVPELTAGIG
                     VSGGTALAGNVGAEQRFEYTVIGDPVNEAARLTELAKEEDGHVLASCDLVEQAGEEEA
                     GHWEAGDEAELRGRSTPTRLARPV"
BASE COUNT          377 a          939 c          880 g          297 t
ORIGIN      
        1 gagatcttct tcgtggacgc cccgctcgcg ggcttcggcg cggtcgagat catcaacacc
       61 gaggacgggg aaccggacga ctaacgtcgc ccgcatgatc gatcagcgac cactcggcac
      121 acagggactg accgcatccg cccagggatt gggctgcatg ggcatgtccg agttctacgg
      181 cgacggcgac gacaccgagt ccgtggccac gatccaccgc gcgatcgagc tcggggtgaa
      241 cttcctggac accgccgcca tgtacggcag cggcgcgaac gagcagctcg tcgggaaggc
      301 catcgccgac cgccgcgacg aggtggtgct ggccacgaag ttcggcatcg tgcgcgggga
      361 ggacccgcgc gagcgcggcg tgaacggcag gcccgagtac gtgaagcagt cgtgtgacga
      421 ctcgctgcgc cgcctgggcc tggaccacgt ggacctcttc taccagcacc gggtggaccc
      481 cgagacgccg atcgaggaga cggtgggcgc catgggtgag ctggtcgacg cgggcaaggt
      541 gcgcttcctg ggcctgtcgg aggcggcgcc ggagaccatc cgccgcgccc acgccaccca
      601 tcccatcagc gcgctgcaga ccgagtactc gctgtggacc cggcacgtgg aggacgagct
      661 gctggagacg gtccgcgagc tgggcatcgg cttcgtggcc tacagcccgc tgggccgcgg
      721 cttcctgacc ggccgcttca agtcggccga cgcgctggac gagaacgact tccgccgcca
      781 caacgaccgc ttcagcggcg acaacctgga cgccaacgtg gcgctggtcg agaaggtcga
      841 ggcgctggcc gccgccaagg aggtcacgcc ggggcagctg gcgctggcct gggtgcatgc
      901 ccagggcgac gacgtcttcc cgatccccgg cactaagcgg cgcgcgtacc tggaggagaa
      961 cgcggccgcg ttcgaggtcg agctgaccgc ggacgacctg gccgcgctgg acgaggcggt
     1021 gcccgccggc gccgccgccg gcgaccgcta cccggccgac ccggagcgct agacggggcg
     1081 ggccagccgc gtgggggtcg agcgcccgcg cagctcggcc tcgtcgccgg cctcccagtg
     1141 gccggcctcc tcctcgcccg cctgctcgac caggtcgcac gaggccagca cgtggccgtc
     1201 ctcctccttg gccagctcgg tcaggcgcgc ggcctcgttc acggggtcgc cgatgacggt
     1261 gtactcgaag cgctgctcgg cgccgacgtt gccggccagg gcggtgcccc ccgacacccc
     1321 gatgccggcg gtcagctcgg gcacctcctc ggccagccgc gcggccagcg accgggccgc
     1381 ggccagcgcc cggtccgccg cgtcgtccag cgggatcggc gcaccgaaca cggccagggc
     1441 cgcgtcgccc tcgaacttgt tgatccagcc gccgtgctcc tcgaccacgt cgaccaccac
     1501 cgcgaagaac ttgttcagca tgtccacgac ctcgtgcggc ggccggtcct gggccaggct
     1561 cgtggagccc acgatgtcgg tgaacagcac ggccacctcg cgcgtctcgc cgcccatctc
     1621 cgtgccctgc tccagcgcgg ccttggccac gtcggtgccg acctgccggc cgaacagctc
     1681 gcggatctcc tcgcgctcgc gcaggcccgc ggccatccgg ttgaagcccg actgcagcag
     1741 cccgagctcg ctgccgtcga acaccgggac ctcggcctcg aagtcgccgt cctcgacccg
     1801 gttcagcgcc tcgcgcaccg acagcaccgg gtcggccgtg ccgcgcgagg cctgccacgc
     1861 cgtcagcagg ccgacggtga gggacacgcc gccgaggcag accgcggtga tggccagctg
     1921 cgtctcggtg acctcgcgcc cggccagcga ggcgatggcc accagcacca gccccacgac
     1981 cgggacgccg gtgcccagcg cccaggcggc cagggcgcgc gtgacgatcc ccgcggtgac
     2041 ctggcggtcg ggcgggcgct gggacagcgc gcgggccacc acggggcgca acagccgctc
     2101 gctgatcagg taggccaccg cgctcgtggt catgcccccc agcgccacga cgatggccac
     2161 caccaacccc agcccgggcg agaacgcgaa gttgacgacg gtgaacacca cgaccgccag
     2221 cgtccacagg aacgagttca ccagcaggat gcggcgcggc gcgcgcaggg tcaggcgctg
     2281 ctcgtcggcg tcgggggcgc ggtccgcgcg cagccaggcg cgcatggggc tcagcctacg
     2341 gtggccccac accacgccca ccgcgatcgc cacgcccagg aacacgccga aggcgatgaa
     2401 gttggccacc agcgcgcccg actcgtcgcc gatgtcgggg cgcgggacca cgtacagcgc
     2461 caggaacagg accacagtgg cgccggcgat gtt
//
LOCUS       DASUPW010000481         4740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_527450, whole genome shotgun sequence.
ACCESSION   DASUPW010000481 DASUPW010000000
VERSION     DASUPW010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527450"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="VFY44_10750"
     CDS             complement(<1..1146)
                     /locus_tag="VFY44_10750"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910965.1"
                     /translation="MALAHTYRGAIGVLAATAALALPTTGEAAQVEVVPYNDGQGHTY
                     LKYTAGPGEDNDLEVVPAVDGVVFREQSVGIDAGPSCAQLTPTSVGCSSEHVQGITIY
                     AGDGSNEVEVEVDLTASIYGAAATKNVFVAGDGRASLVGGEGDDELYGGAGINDIRGY
                     GGDDLISGGPGEDDMEGGQGDDTFTADAGADRMNGDGGHDTVDYSARTKPVAVFFDGS
                     YNSGAIAGPYSLAEHDVVGESVEAAIGGTANDTLVGSSDPNRLTGGPGDDSLDTRGGG
                     VDVADCGDGTDTALTDADDTRTACELPAPAVAQPPMQPPPAPSSSPLQPAVRPAAPKS
                     AGPKVRIGPARLRIGILGRVPVRIRCPRTAVRHCTGVLSLGAGTRARP"
     gene            complement(1150..1413)
                     /locus_tag="VFY44_10755"
     CDS             complement(1150..1413)
                     /locus_tag="VFY44_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910966.1"
                     /translation="MLTHAHPTDQPEAVTVRLGRATDSGQLADLAGLNCAAVPEGAVM
                     VAETGGSIVAAVPVDGGPAIADPFRRTAAVISLLKLRAAQMGA"
     gene            complement(1443..2855)
                     /locus_tag="VFY44_10760"
     CDS             complement(1443..2855)
                     /locus_tag="VFY44_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910967.1"
                     /translation="MLICPQAAGAADVGVASMGNGQNHVYLFSAPGEDNDLEILPGPS
                     SVVLHDAEAPVTTVDPACAQTDANTVTCSDDGVARVDATMRDGTNEVRVEGGLPARVD
                     ADPESARNVFTSATSQAVTFRGGNGNDLLQGGGGDDDIDGGGGPDQVGGGGGDDQLRG
                     GTGNDTFLAEGGADTVHGDGGYDYMDYSTRPSRVRVSLDGLANDGSPLGPVQVAEFDN
                     VTGVEAVFGGSNDDTLSGTTGPELLIGNGGDDTLAGGGGADQLYGYAGSDRIDGDGGD
                     DTAVGGEGSDTLVGGAGVDQIEGREGDDVLDARDDAADTADCGPGADTAQTDPGDARL
                     ACELPAPAVASPPLSPKPPAAAPKGPARLKMRIGPARVKLGRSGKVALKVRCPADAPG
                     RCSGVLSLTAKSGRKAGSAGRARFSAAPGKTATVRIRLSRSVRAKARRRALKLAVRAV
                     TATAGSGSTTTQGSVTLARR"
     gene            3002..4459
                     /locus_tag="VFY44_10765"
     CDS             3002..4459
                     /locus_tag="VFY44_10765"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX5910968.1"
                     /translation="MAAIDRDAARLRELGYEQELDRRLGGLGNVVVGFATTSPVVGLY
                     AVVLLGTLVAGPAWVWVLPVALLGQCLLLAVYAELAAEFPITGGAYQWTRRLIGPAYG
                     WFTGWVAVCAYAAANTTIAYLGAPWALTLLDVDPTPGRLVLAGVALALLCALVGAAGI
                     GALRWVLGIGVTAEVVALVGIGLVLLLAFRDQEFSLLGGTLGAEAASGGSVFAALLAA
                     LAVSGWVFIGFDACVGSAEETKGAARRVPRSVWLVVLAVAVIVLLNAVATALAHPDPG
                     AVVGGKDVDPVTTAVVEAFGSWSAKPFAALVLGAFLSCGIAAQALTARTMYSIARDGV
                     MPGSRVLRRVNRRGTPIAAVVVTTVLACAGLLLALESAAIGSIVIFGTASIYATFLLI
                     ATAALVARVRGTWTPAGHVQLGRLGTAVNALAVAWLAFELVNIAWPREVLAPVGAPFY
                     QVWAAPLLLAAITTSGLAYLLIARPHRALAGAAGV"
     gene            4463..>4740
                     /locus_tag="VFY44_10770"
     CDS             4463..>4740
                     /locus_tag="VFY44_10770"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5910969.1"
                     /translation="MATASGRLVGREAELAVVEGALDALTGPEPALIDIVGEAGIGKT
                     RIFGEVCARAQARDFLVLRGRAAEFEQRVPFAAVVEAMDGHLGTLGPA"
BASE COUNT          490 a         1713 c         1826 g          711 t
ORIGIN      
        1 gggccgggcc cgcgtgccgg cgccgagcga cagcacgccg gtgcagtggc ggaccgccgt
       61 cctggggcag cggatccgca cgggcacacg cccgagaatg ccgatgcgca ggcgcgcggg
      121 accgatacgg accttcggtc cggcggactt cggtgccgcc ggacggaccg cgggctgcaa
      181 cggcgaggac gacggtgccg gcggcggctg catcggcggc tgggcgactg ccggcgcagg
      241 cagctcgcat gcagtgcggg tgtcgtcggc gtcggtcaac gccgtgtcgg tgccgtcgcc
      301 gcagtcggcc acgtccacac cgcccccgcg ggtgtccagg gagtcgtccc cgggcccgcc
      361 ggtcagccgg ttggggtcgg aggaccccac cagggtgtcg ttggcggtgc cgccgatggc
      421 cgcctcgacg gactcgccga ccacgtcgtg ctcggcaagc gagtacgggc cggcgatggc
      481 gccgctgttg tacgagccgt cgaagaacac cgccacgggc ttcgtgcggg cggagtagtc
      541 gacggtgtcg tggccgccgt cgccgttcat gcggtcggca ccggcgtcgg cggtgaaggt
      601 gtcgtcgccc tgaccgccct ccatgtcgtc ctcgcccggg ccgcccgaga tgaggtcgtc
      661 cccgccgtag ccccggatgt cgttgatccc ggcgccgccg tagagctcgt cgtctccctc
      721 cccgccgacc agggaggcgc gcccgtcgcc cgccacgaac acgttcttcg tggcggccgc
      781 gccgtagatg ctggcggtca ggtcgacctc gacctcgacc tcgttcgacc cgtcgccggc
      841 gtagatggtg atcccctgga cgtgctcgct cgagcagccc accgacgtgg gcgtcagctg
      901 ggcgcacgag gggccggcgt cgatgccgac cgactgctcg cggaacacga ccccgtccac
      961 cgccggcacc acctcgaggt cgttgtcctc gcccgggccg gccgtgtact tgagatacgt
     1021 gtggccctgg ccgtcgttgt agggcacgac ctcgacctgc gccgcctcgc ccgtggtggg
     1081 cagggccagc gcggcggtcg cggccagaac accgatcgcg ccgcggtatg tgtgggcgag
     1141 ggccatggct caggccccca tctgcgcggc gcgcagcttc aggaggctga tcaccgcggc
     1201 cgtgcgccgg aaggggtcgg cgatggccgg cccgccgtcg acgggcacgg ccgcgacgat
     1261 ggacccgccg gtctcggcca ccatcaccgc gccctccggt acggcggcgc agttcagtcc
     1321 ggccagatcg gccagctggc ccgagtcggt ggcccggccc agccggacgg tcaccgcctc
     1381 gggctgatcg gtggggtgtg cgtgcgtgag catgggatcg tctcctctgg tctgggtggg
     1441 ggtcagcggc gggcgagcgt gacgctgccc tgggtggtcg tggagccgga gccggcggtg
     1501 gccgtgacgg ctcgcaccgc cagcttcagc gcgcgcctgc gggccttggc ccggaccgag
     1561 cgcgacagcc ggatccgcac cgtcgcggtc ttgcccggtg ccgccgagaa gcgcgcccgc
     1621 ccggcgctgc ccgccttgcg gccgctcttg gcggtcaggc tcagcacccc gctgcagcgg
     1681 ccgggggcgt cggcgggaca gcgcaccttc agcgccacct tgccgctcct gcccagcttc
     1741 acccgcgccg ggccgatccg catcttcagg cgggccgggc ccttcggcgc ggccgcgggg
     1801 ggcttggggc tcagcggcgg gctcgccacc gccggcgcgg gcagctcgca cgccaggcgg
     1861 gcgtcgcccg ggtcggtctg cgccgtgtcg gcgcccgggc cgcagtccgc cgtgtcggcg
     1921 gcgtcgtcgc gggcgtccag cacgtcgtcg ccctcgcggc cctcgatctg gtcgaccccc
     1981 gcgccgccga ccagcgtgtc ggagccctct cccccgaccg ccgtgtcgtc gccgccgtcg
     2041 ccgtcgatgc ggtcgcttcc ggcgtacccg tacagctggt cggcgccacc gccgccggcg
     2101 agcgtgtcgt caccgccgtt gccgatcagc agctccggcc cggtcgtgcc cgacagcgtg
     2161 tcgtcgttcg agccgccgaa gacggcctcc acgccggtga cgttgtcgaa ctcggccacc
     2221 tggaccggcc ccagcggcga gccgtcgttg gccaggccgt ccagcgacac ccgcacccgg
     2281 ctgggacggg tcgagtagtc catgtagtcg tacccgccgt cgccgtggac cgtgtcggcg
     2341 ccgccctcgg cgaggaacgt gtcgttgccg gtgccgcccc gcagctggtc gtcgcctcct
     2401 cctccgccga cctggtcggg accgccgccg ccgtcgatgt cgtcgtcacc gccgccgccc
     2461 tgcagcaggt cgttgccgtt tccaccgcgg aaggtcacgg cctgcgaggt cgccgacgtg
     2521 aacacgttcc tggccgactc cgggtcggcg tccaccctgg ccggcagccc gccctcgacc
     2581 cggacctcgt tggtgccgtc gcgcatggtc gcgtcgaccc gggccacgcc gtcgtccgag
     2641 caggtgacgg tgttggcgtc cgtctgcgcg catgccgggt cgaccgtggt caccggggcc
     2701 tcggcgtcgt ggaggaccac gctggagggt ccgggcagga tctccaggtc gttgtcctcg
     2761 cccggcgccg agaagaggta gacgtggttc tgcccgttgc ccatcgacgc gacgcccacg
     2821 tcggccgctc cggccgcctg gggacagatg agcacggccg tgacgacggc accggccaga
     2881 agcccggtcc gtcgggtgga tgtggggcgt tgagtgaaca tggcccgatc gtggatcgcc
     2941 accggccccg ggtcatcagg ggccatccct gaaccgcccc tgagtgggtt agggtcgctc
     3001 gatggctgcg atcgaccgcg atgcggcgcg cctacgggaa ctgggctacg aacaggaact
     3061 cgaccgccgg ctggggggcc tcggcaacgt ggtggtgggc ttcgccacga cctcgccggt
     3121 ggtggggctg tacgccgtgg tgctgctggg gaccctggtc gccggcccgg catgggtctg
     3181 ggtgctgccg gtggcgctgc tgggccagtg cctgctgctg gccgtgtacg ccgagctggc
     3241 ggcggagttc ccgatcaccg gtggcgccta ccagtggacc cggcgcctga tcggtcccgc
     3301 ctacggctgg ttcacgggct gggtggcggt gtgcgcgtac gccgcggcca acaccacgat
     3361 cgcctacctg ggggcgccgt gggcgctgac gctcctggac gtggacccca cgcccggccg
     3421 gctggtgctg gccggtgtgg cgcttgcgct gctgtgcgcg ctggtgggcg ccgccgggat
     3481 cggggcgctc aggtgggtcc tggggatcgg cgtgaccgcc gaggtggtgg cgctggtcgg
     3541 catcggcctg gtgctgttgc tggccttccg cgaccaggag ttctcgctgc tgggcgggac
     3601 gctgggcgcc gaggccgcgt cggggggatc ggtgttcgcc gcgctgctgg cggcgctggc
     3661 ggtcagcggg tgggtcttca tcgggttcga cgcctgcgtg gggagcgcgg aggagaccaa
     3721 gggcgcggcg cggcgcgtgc cccgatcggt ctggctcgtg gtgctggccg tggccgtgat
     3781 cgtgctgctg aacgccgtcg ccacggcgct ggcccatccg gatccgggcg ccgtggtcgg
     3841 gggcaaggac gtggacccgg tcacgacggc ggtcgtcgag gcgttcggct cgtggtcggc
     3901 caagccgttc gccgctctgg tgctgggggc gttcctgtcg tgcggcatcg ccgcgcaggc
     3961 gctgacggcg cgcaccatgt actcgatcgc acgcgacggg gtgatgcccg gctcccgggt
     4021 gctgcgccgg gtgaaccgca ggggcacgcc catcgccgcc gtggtggtga ccaccgtgct
     4081 ggcctgcgcc ggcctgctcc tggccctgga gtcggccgcc atcggcagca tcgtcatctt
     4141 cggcaccgct tccatctacg ccacgttcct gctgatcgcg accgccgcgc tggtggcgcg
     4201 cgtgcgcggc acgtggacgc ccgccggcca tgtgcagctg ggccgcctgg gcaccgccgt
     4261 caacgcgctg gcggtggcgt ggctggcctt cgagctggtc aacatcgcat ggccgcgcga
     4321 ggtgctggcg cccgtgggcg cgccgttcta ccaggtgtgg gcggctccgc tgctcctggc
     4381 ggccatcacc accagcggcc tggcctacct gctgatcgcc aggccccacc gggcgctggc
     4441 cggggccgcc ggggtctagg gcgtggccac cgcctccggc cggctggtgg gacgcgaggc
     4501 cgagctcgcg gtggtcgagg gcgccctcga cgccctgacc ggccccgagc cggccctgat
     4561 cgacatcgtg ggcgaggcgg gaatcggcaa gacgcggatc ttcggcgagg tgtgcgcgcg
     4621 ggcgcaggcg cgtgacttcc tggtcctgcg gggacgggcc gcggagttcg agcagcgggt
     4681 cccgttcgcc gcggtggtgg aggcgatgga cggccacctg gggacgctcg gcccggccag
//
LOCUS       DASUPW010000482         5564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1912963, whole genome shotgun sequence.
ACCESSION   DASUPW010000482 DASUPW010000000
VERSION     DASUPW010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1912963"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VFY44_10775"
     CDS             <1..829
                     /locus_tag="VFY44_10775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5910970.1"
                     /translation="VTALQGMAWLVRRLSETGGRLSRSNTDLELANANLARSNADLEQ
                     FAYVASHDLSSPLRSVAGFSQMLARRYRGRLDADADTWIGFVEQGVGRMQRVIDDLLL
                     YSRVDREEPDPEPVDLDEVLDEVRRSLGPQLDDKGGELTHDPLPAVLAEHTHMVQVLQ
                     NLVGNALTYVAPGVEPRVHVSATPEGGRARIAVTDNGVGVDPEHVDRIFKMFQRLHAE
                     DEHPGTGIGLAITKKIVERYGGRIEVAAAPGGGSTFSFTIAAAPVAPPRREREKVLR"
     gene            826..1227
                     /locus_tag="VFY44_10780"
     CDS             826..1227
                     /locus_tag="VFY44_10780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5910971.1"
                     /translation="MTRVLLVEDNEADVVLLREALREGGVDLDLTVAPDGEQAMAGLR
                     NGGGLPDLVLLDLNLPRKDGREVLSEMKADPALAGVPVIVLTTSQSPGDVAFAYRNHA
                     NAYVRKPNGLDQLIEVTRAIRDFWVAAATLP"
     gene            1244..1894
                     /locus_tag="VFY44_10785"
     CDS             1244..1894
                     /locus_tag="VFY44_10785"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX5910972.1"
                     /translation="MTDFSDLRALYVNCTLTRSPEPSHTQRLIDQSAAIMEEQGVSVD
                     QFRAVDHDIATGVQPDMTEAGWDTDEWPELFQRVLAADILVLGGPIWLGDNSSQTKLV
                     IERLYSNSSDLNERGQSVFYGRAGGCLITGNEDGIKHCASNVLYSLQHVGYTIPPQAD
                     AGWIGEAGPGPSYGDDGAGLDNEFTRTNTGFMAWNLMHLARMLKGGIPAQGNVREQ"
     gene            complement(1897..2367)
                     /locus_tag="VFY44_10790"
     CDS             complement(1897..2367)
                     /locus_tag="VFY44_10790"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HEX5910973.1"
                     /translation="MPPDQPPTLYEWAGGTAAFERWLNAFYDQLELDEELVALFGGRV
                     SEEHRRHVVAWWAEVMGGPSTYTDELGGYERMLGHHRGLQITPEQRLRFVTLLSQAAD
                     VAALPPDPEFRAALMGYAEWGSRLALHNSDPGAEVATSAPVPRWGWGVAPPYQP"
     gene            complement(2357..3850)
                     /locus_tag="VFY44_10795"
     CDS             complement(2357..3850)
                     /locus_tag="VFY44_10795"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5910974.1"
                     /translation="MDHVDVLVVGAGISGIGAACHLEDECPGKTYAVLEARDSIGGTW
                     DLFRYPGIRSDSDMFTLGYRFKPWTDTKTLADGPAILSYVRETAREHGVEDKIRYGHK
                     VVGLSWSGDDARWTVRIERDGEPAEMTAGFVFLCSGYYDYEGGYTPEFPGVDRFEGEL
                     IHPQKWPEDLDYAGKRVVVIGSGATAVTMVPAMADTAAHVTMLQRSPTYIVALPAVDP
                     IARAVRRVLPLKAAYMVSRWKNVLLQMVTFNLSRWRPNLVRRAIRAAIKRQLPAGYDV
                     DRHFKPPYDPWDQRMCVVPDADLFRSLSSGRAEMVTDTIETFTERGIRLASGRELEAD
                     IVVSATGLNLKALGALDVTVDGEPVPIPDRLTYKSLMLEDVPNLAFTIGYTNASWTLK
                     ADLTSEYVCRLVNHMDRHGYDVCVPHNDDPSVTREPLIDLDSGYVRRSLDQMPKRGSK
                     EPWRLRQNSAVDIVNLRHGKLEDGTLRFTRRGEAAAAGTAAEHTVAA"
     gene            complement(3850..4155)
                     /locus_tag="VFY44_10800"
     CDS             complement(3850..4155)
                     /locus_tag="VFY44_10800"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEX5910975.1"
                     /translation="MLNLLRFDGDEGRASYARYGEAVVPMVEAVGGEVVFSGRCSDAV
                     IGPQEGEWDSVLVVRYPSRRAFLDMISSEEYRRDAHPLRAAAIADSRLIPCAEGAAG"
     gene            complement(4268..5281)
                     /locus_tag="VFY44_10805"
     CDS             complement(4268..5281)
                     /locus_tag="VFY44_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910976.1"
                     /translation="MTWRPAAALLAVLLTAPSTAQAVPGGAPLNVPPSTNSGCEALVI
                     PVQGQPTPPSCTLLGIDPAGTWTSQTPRGNWVITTARVRTGPRVGPMVFTVIRATRSQ
                     AGTGAGRIICCSVPVESQVLRPRPNAINEVPVRLPVKNTVEVIGGEPIEVVDYLGISL
                     LDLSSSGPVAASSNETVSWIAPALRQGGEAIQGGAFNARVLVNGEYEPADAAGGGGGG
                     GGGVADRVAPALTRLGLSRSRFAAAGRGGSIAAKVGTTIRYRQSEAATVTFRVRRKGA
                     RKPLKGSFAHRGKAGANKLRFTGRLRGKKLRRGSYVLSATSKDAAGNVAKVKRLRFRI
                     VKR"
     gene            5339..>5564
                     /locus_tag="VFY44_10810"
     CDS             5339..>5564
                     /locus_tag="VFY44_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910977.1"
                     /translation="MTSLLDDLNDWLRIPSVSIGDPDHAALEQAAQWVVDRVRAAGGE
                     AGQVVVDGGNPLAVGELRAADHGAPTVLIYG"
BASE COUNT          782 a         2002 c         2002 g          778 t
ORIGIN      
        1 ggtgaccgcg ctccagggca tggcctggct ggtccgccgc ctgtcggaga cgggcggtcg
       61 cctcagccgg tccaacaccg acctcgagct ggccaacgcg aacctggccc ggtcgaacgc
      121 cgacctcgag cagttcgcct acgtggcctc gcacgacctg tcgtcgccgc tgcggtcggt
      181 ggccgggttc agccagatgc tggccaggcg ctaccgcgga cggctggacg ccgacgccga
      241 cacgtggatc gggttcgtcg agcagggcgt cggccggatg cagcgcgtga tcgacgacct
      301 gctcctgtac tcccgcgtgg accgtgagga gccggacccc gagcccgtgg acctggacga
      361 ggtgctggac gaggtccggc gctcgctggg cccgcagctg gacgacaagg gcggggagct
      421 cacgcacgac ccgctgcccg cggtgctggc cgagcacacg cacatggtcc aggtgctgca
      481 gaacctggtc ggcaacgcgc tgacctacgt ggctccgggg gtcgagccgc gggtgcacgt
      541 gtccgccacg cccgagggcg gtcgcgcgcg gatcgcggtg accgacaatg gcgtcggggt
      601 cgaccccgag cacgtggacc gcatcttcaa gatgttccag cggctgcacg ccgaggacga
      661 gcacccggga acggggatcg gcctggccat aaccaagaag atcgtcgagc gctacggcgg
      721 tcgcatcgag gtcgcagccg cgccgggagg cggcagcacc ttctccttca cgatcgccgc
      781 ggcgccggtc gccccgccgc gccgcgagag ggagaaggtg ctgcggtgac ccgtgtcctg
      841 ctggtcgagg acaacgaggc cgacgtcgtc ctgctccggg aggccctgcg cgagggcggg
      901 gtcgacctgg acctgaccgt cgcgcccgac ggcgaacagg cgatggccgg cctgcgcaac
      961 ggtggcgggc tgcccgacct ggtgctcctg gacctgaacc tgccgcgcaa ggacgggcgc
     1021 gaggtgctgt ctgagatgaa ggccgacccg gcgctggccg gcgtgccggt gatcgtgctg
     1081 accacctcgc agtcacccgg cgacgtggcg ttcgcctacc gcaaccacgc caacgcgtac
     1141 gtgcgcaagc cgaacggcct ggaccagttg atcgaggtca cacgcgccat tcgcgacttc
     1201 tgggtggccg ccgccacgct gccgtagcta gcgtcgtcgg ccgatgaccg acttcagcga
     1261 cctccgcgcc ctgtacgtga actgcacgct gacgcgctcg cccgagccca gccacaccca
     1321 gcgcctgatc gaccagagcg cggcgatcat ggaggagcag ggcgtgagcg tggaccagtt
     1381 ccgcgccgtc gaccacgaca tcgccaccgg cgtgcagccg gacatgaccg aggccggctg
     1441 ggacaccgac gagtggccgg agctgttcca gcgtgtgctg gccgccgaca tcctggtgct
     1501 gggcggcccc atctggctgg gcgacaacag ctcgcagacc aagctggtca tcgagcgcct
     1561 gtactcgaac tcctccgacc tgaacgagcg cgggcagtcg gtgttctacg gccgcgccgg
     1621 cggctgcctg atcaccggga acgaggacgg catcaagcac tgcgcctcga acgtgctcta
     1681 cagcctccag cacgtgggct acacgatccc gccgcaggcc gacgccggct ggatcggcga
     1741 ggcgggcccc ggccccagct acggcgacga cggcgccggg ctggacaacg agttcaccag
     1801 gaccaacacc gggttcatgg cctggaacct gatgcacctg gcgcggatgc tcaagggcgg
     1861 gatcccggcc cagggcaacg tccgcgagca gtagggctag ggctggtagg gcggcgccac
     1921 gccccagccc cagcggggca ccggcgccga ggtggccacc tcggcgcccg gatccgagtt
     1981 gtgcagcgcc agccgcgatc cccactccgc gtagcccatg agcgccgcgc ggaactcggg
     2041 gtccggcggc agcgccgcca cgtcggcggc ctggctgagc agcgtgacga agcgcagccg
     2101 ctgctcgggg gtgatctgca ggccgcggtg gtggcccagc atccgctcgt agccgcccag
     2161 ctcgtcggtg taggtggagg ggccgcccat gacctccgcc caccaggcca cgacgtgccg
     2221 gcggtgctcc tccgacacgc gcccgccgaa cagggccacc agctcctcgt ccagctcgag
     2281 ctggtcgtag aacgcgttca gccagcgctc gaacgccgcg gtcccgcccg cccactcgta
     2341 cagcgtcggt ggctggtcag gcggcaacgg tgtgctcggc cgccgtgccc gcggcggccg
     2401 cctcgccccg gcgggtgaac cgcagcgtcc cgtcctcgag cttgccgtgg cgcaggttca
     2461 cgatgtccac ggcggagttc tggcgcagcc gccacggctc tttcgagccg cgcttgggca
     2521 tctggtccag cgaccgccgc acgtagcccg agtccaggtc gatcagcggc tcgcgcgtca
     2581 cgctcgggtc gtcgttgtgg ggcacgcaca cgtcgtagcc gtggcggtcc atgtggttca
     2641 ccaggcggca gacgtactcc gacgtcaggt cggccttcag cgtccaggag gcgttggtgt
     2701 agccgatggt gaaggccagg ttgggcacgt cctcgagcat caggctcttg taggtcagcc
     2761 ggtcggggat gggcacgggc tcgccgtcca cggtcacgtc cagcgcgccc agggccttga
     2821 ggttcaggcc ggtggcgctg accacgatgt cggcctccag ctcccgcccg gaggccaggc
     2881 ggatgccgcg ctcggtgaac gtctcgatgg tgtcggtcac catctcggcg cgcccgctgc
     2941 tgagggaccg gaacaggtcc gcgtcgggca ccacgcacat gcgctggtcc caggggtcgt
     3001 agggcggctt gaagtggcgg tcgacgtcgt agccggcggg cagctggcgc ttgatggcgg
     3061 cgcggatggc ccggcgcacc aggttcgggc gccaccggct gaggttgaag gtgaccatct
     3121 gcagcagcac gttcttccag cgcgagacca tgtacgcggc cttgagcggc agcacgcggc
     3181 ggaccgccct ggcgatcggg tccacggcgg gcagcgccac gatgtaggtc ggcgagcgct
     3241 ggagcatcgt cacgtgcgct gccgtgtcgg ccatggccgg gaccatcgtc acggccgtcg
     3301 cgccgctgcc gatgaccacc acgcgcttgc ccgcgtagtc caggtcctcg ggccacttct
     3361 gcgggtggat cagctcacct tcgaagcgat cgacgcccgg gaactccggc gtgtagccgc
     3421 cctcgtagtc gtagtagccg ctgcacagga acacgaagcc ggccgtcatc tcggcgggct
     3481 cgccgtcgcg ctcgatccgc accgtccagc gcgcgtcgtc gcccgaccag ctcagcccga
     3541 cgaccttgtg gccgtaccgg atcttgtcct cgaccccgtg ctcgcgcgcg gtctcgcgca
     3601 cgtaggacag gatcgccggg ccgtcggcca gggtcttggt gtcggtccag ggcttgaacc
     3661 ggtagcccag cgtgaacatg tcggagtccg agcggatgcc cgggtagcgg aacaggtccc
     3721 acgtgccgcc gatggagtcg cgggcctcga gcaccgcgta ggtcttgccc gggcactcgt
     3781 cctccaggtg acaggcggcg ccgatgccgg agatcccggc gcccaccacc aggacgtcca
     3841 cgtggtccat cagcccgcgg ccccctcggc gcaggggatc agccgcgagt cggcgatcgc
     3901 ggccgcccgg agcgggtgcg cgtcgcgccg gtactcctcg ctggagatca tgtccaggaa
     3961 cgcccgccgc gacgggtagc gcaccaccag cacggagtcc cactcgccct cctgcgggcc
     4021 gatcaccgcg tcggagcagc ggcccgagaa caccacctcg ccgcccacgg cctcgaccat
     4081 cggcacgacg gcctcgccgt agcgcgcgta cgacgcgcgg ccctcgtccc cgtcgaagcg
     4141 caggaggttg agcatgacca ccggtgaggc gtccgcctct gcggcggcgg tcagtgcctc
     4201 gagctgctcc cgcgtggtgt cggggaaggc cgcgatgggg ggagcttacg gcctcggcgt
     4261 gtccggttca gcgcttgacg atccggaagc gcagccgctt gaccttggcc acgttgcccg
     4321 cggcgtcctt cgacgtggcc gacaacacgt acgacccgcg cctgagcttc ttgccgcgca
     4381 gccggccggt gaagcgcagc ttgttggccc ccgccttgcc cctgtgggcg aatgagccct
     4441 tgagcggctt gcgcgctccc ttgcgccgca cgcggaaggt cacggtggcc gcctcgctct
     4501 gccggtagcg gatcgtggtg cccaccttcg cggcgatgct gccgccgcgg ccggcggcgg
     4561 cgaagcggct gcgcgagagc ccgaggcggg tcagggcggg ggcgacccgg tcggccacgc
     4621 caccgccgcc acccccaccg ccgcccgccg cgtcggcggg ctcgtactcg ccgttgacca
     4681 gcacgcgggc gttgaaggcg ccgccctgga tcgcctcgcc gccctgccgg agcgcgggcg
     4741 cgatccacga cacggtctcg ttcgagctgg cggccacggg acccgagctg ctcaggtcca
     4801 gcaggctgat gcccaggtag tccacgacct cgatcggctc gccgccgatc acctcgacgg
     4861 tgttcttgac ggggagccgc accgggacct cgttgatcgc gttgggccgt ggcctcaaca
     4921 cctggctctc gacgggcacg ctgcagcaga tgatccgccc ggcgcccgtc ccggcctggg
     4981 agcgggtggc gcggatgacg gtgaacacca tcgggccgac gcgcgggccc gtgcgcacgc
     5041 gcgcggtggt gatgacccag ttgccccggg gcgtctgcga ggtccacgtg cccgcggggt
     5101 cgatgcccag cagcgtgcag ctgggcgggg tcggctgccc ctgcaccggg atgaccagcg
     5161 cctcgcaccc gctgttggtg ctcggcggta cattcagcgg cgccccaccg ggcacggcct
     5221 gggccgtcga aggcgcggtc agcaagaccg ccaggagtgc cgctgcgggc cgccaggtca
     5281 tcgacgatcc aacgtacacc cgcaccgggt agcccgctat acggcagatg aggtacatgt
     5341 gacatccctt ctggacgatc tgaacgactg gctgcggatc ccgtcggtgt ccatcggcga
     5401 tcccgatcac gctgcgctgg agcaggccgc gcagtgggtg gtggaccgcg tccgggccgc
     5461 cggcggcgag gccgggcagg tggtcgtcga cggcggcaac ccgctggccg tcggcgagct
     5521 gcgcgccgcc gaccacgggg cccccacggt gctgatctac ggcc
//
LOCUS       DASUPW010000483         2114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_804603, whole genome shotgun sequence.
ACCESSION   DASUPW010000483 DASUPW010000000
VERSION     DASUPW010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804603"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VFY44_10815"
     CDS             <1..384
                     /locus_tag="VFY44_10815"
                     /inference="COORDINATES: protein motif:HMM:NF017847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HEX5910978.1"
                     /translation="RIEAELSELDDEEEAAAMREDLGAGESGLATVVREAWKLLGLIT
                     FFTAGEGKEGRAWAIERGTAARRAAGKIHTDIEKGFVAAEVVPWDALVQTGGYAAARE
                     QAKLRIEGRDYEVQDGDVITVRFTP"
     gene            381..1670
                     /locus_tag="VFY44_10820"
     CDS             381..1670
                     /locus_tag="VFY44_10820"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX5910979.1"
                     /translation="MSDRRPHLAWAGGLAAAGLAWYSADSLRHRREEGSSGYELAGEV
                     SVTDPTFMRAAEALTAAPIAEGNHVELLVNGDAIFPRYLETIAGAQRTLNITTYAYWK
                     GQIAIDVAEALIERAKAGVEVNVLIDALGGAKMDRSLLGDMEDAGVTVHRFRPLKPYA
                     VRRLENRTHRKLLIADGRVGMTGGVGIAEEWTGNAQDPDHWRDTHVRVEGPVVRGLQG
                     AFAENWIEATGRVLAGEDYLPDIEPFEDGVPMQVTRSSAGVGDTNAEALTYLAIAAAD
                     RTLDLTSAYFVPRPAFLQALVDAAERGVQLRILVPGSHIDKGIVRTAGRSVYDQLLEV
                     GAELYEYGPTMLHAKTLTVDGAWASVGSVNFDNRSFQLHDEAALSVRDEAFATALTEQ
                     FERDIAVSERIEPEAWAERGPRRRAAEAVSRLARREL"
     gene            1667..>2114
                     /locus_tag="VFY44_10825"
     CDS             1667..>2114
                     /locus_tag="VFY44_10825"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX5910980.1"
                     /translation="MSVRLALWANPASGGSTDEGEVEDALRDRGAELVDEQDAERLVV
                     AGGDGSVAPAAERAQELGVPLAVVPTGTANDFARAAGLPDDLEAACELAATGSTIRTL
                     DLGRKDGRPFVTACSAGLAVDAARSASGLKKALGPLAYAVGALHAGA"
BASE COUNT          302 a          739 c          830 g          243 t
ORIGIN      
        1 cggatcgagg ccgagctgtc cgagctggac gacgaggagg aggccgcggc gatgcgcgag
       61 gacctgggcg cgggcgagtc gggcctggcc accgtggtcc gcgaggcgtg gaagctgctg
      121 gggctgatca ccttcttcac cgccggcgag ggcaaggagg gacgggcgtg ggccatcgag
      181 cgcggcaccg cggcccgccg cgccgcgggc aagatccaca ccgacatcga gaaggggttc
      241 gtggcggccg aggtggtgcc ctgggacgcg ctggtgcaga ccggtggcta cgccgccgcg
      301 cgcgaacagg ccaagctccg gattgagggg cgcgactacg aggtacagga cggggacgtg
      361 atcaccgtcc gcttcacccc atgagtgacc gccgccccca tctggcgtgg gccggcggcc
      421 tggccgcggc cgggctggcc tggtactcgg ccgactccct gcgccaccgg cgcgaggagg
      481 gcagcagcgg ctacgagctg gccggcgagg tgtcggtcac cgacccgacc ttcatgcgcg
      541 ccgccgaggc gctgaccgcc gcgccgatcg ccgagggcaa ccacgtcgag ctgctggtca
      601 acggcgacgc catcttcccg cgctacctgg agaccatcgc gggcgcgcag cggaccctga
      661 acatcaccac ctacgcgtac tggaagggcc agatcgccat cgacgtggcc gaggcgctga
      721 tcgagcgcgc gaaggccggc gtcgaggtca acgtgctgat cgacgccctc ggcggcgcga
      781 agatggaccg ctcgctgctg ggcgacatgg aggacgccgg cgtgaccgtg caccgcttcc
      841 gcccgctcaa gccctatgcg gtccggcggc tggagaaccg cacccaccgc aagctgctga
      901 tcgccgacgg ccgggtgggc atgaccggcg gcgtgggcat cgccgaggag tggaccggca
      961 acgcccagga ccccgaccac tggcgcgaca cccacgtccg cgtcgagggc cccgtggtgc
     1021 gcgggctcca gggggcgttc gccgagaact ggatcgaggc caccggcagg gtgctggcgg
     1081 gcgaggacta cctgccggac atcgagccgt tcgaggacgg cgtgccgatg caggtcacgc
     1141 gctcgagcgc cggcgtcggc gacaccaacg ccgaggcgct gacctacctg gccatcgccg
     1201 cggccgaccg cacgctggac ctgacctcgg cgtacttcgt gccccggccc gcgttcctgc
     1261 aggccctggt cgacgccgcc gagcgcggcg tccagctgcg gatcctggtg ccgggctcgc
     1321 acatcgacaa gggcatcgtg cggaccgccg gccggtcggt ctacgaccag ctgctggagg
     1381 tgggtgccga gctgtacgag tacggcccca cgatgctgca cgccaagacg ctgacggtgg
     1441 acggcgcatg ggccagcgtg gggtcggtga acttcgacaa ccgctcgttc cagctgcacg
     1501 acgaggcggc cctgtccgtg cgcgacgagg ccttcgccac ggcgctgacg gagcagttcg
     1561 agcgcgacat cgccgtctcg gagcggatcg agcccgaggc atgggccgag cggggaccgc
     1621 gccggcgggc cgccgaggcc gtcagccgcc tggcgcggcg cgagctgtga gcgtgcgcct
     1681 ggcgctgtgg gccaacccgg cgtcgggcgg cagcaccgac gagggtgagg tggaggacgc
     1741 cctgcgcgac cgcggcgcgg aactggtgga cgagcaggac gccgagcggc tggtggtggc
     1801 cggcggggac gggagcgtgg ccccggccgc ggagcgcgcg caggagctcg gcgtgccgct
     1861 ggccgtggtg cccaccggca ccgccaacga cttcgcccgc gccgccgggc tgccggacga
     1921 cctggaggcg gcctgcgagc tggccgccac gggcagcacg atccgcacgc tcgacctggg
     1981 ccgaaaggac gggcggccgt tcgtgaccgc gtgcagcgcc gggctcgcgg tggacgcggc
     2041 ccgatcggca tcgggcctca agaaggcgct ggggccgctg gcgtacgcgg tcggggcgct
     2101 gcacgccggg gcca
//
LOCUS       DASUPW010000484         2441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_2053056, whole genome shotgun sequence.
ACCESSION   DASUPW010000484 DASUPW010000000
VERSION     DASUPW010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2053056"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VFY44_10830"
     CDS             <1..286
                     /locus_tag="VFY44_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910981.1"
                     /translation="NVNRMCAGVLQVVGGAVELVLDGRPRRVAAGQYAEVPRDVPWRA
                     VVEADAHVLWFGAPAGIESLVGMLGHPPLDDDDAAALLAAAGVSRVRASW"
     gene            complement(278..1402)
                     /locus_tag="VFY44_10835"
     CDS             complement(278..1402)
                     /locus_tag="VFY44_10835"
                     /inference="COORDINATES: protein motif:HMM:NF017856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEX5910982.1"
                     /translation="MSDRSDRLREAHAVAAVRSSARRRMDRVTGAWLEILQTGVAAAV
                     AWQAAKLIVGQSQPFFAPIAAVLALGLSRGQPRRRAVELGLGVALGILIADLLVQLIG
                     TGTWQIGVVVVLAMSAALVVGAGILLVNQAAISAILVITLPSLSQGGTLDRLFDALIG
                     AAVALVLSQVLFPRDPVRQMGKAAGRVLDELALALEETAVALADADQTAAEHALERVR
                     ALDDAIGAFYDAVAVGRENAWLSPARRRRSLGHLRSYADAARQVDYAVRNTRILARNA
                     AQAARTGPPDQELADAVSILADAVRALGRELATPDQEDEETRYLARQAADQATSVLGR
                     RSDLRTNMVVGQVRATATDLLRGTGLDTQAARLTPPGTSP"
     gene            complement(1399..2286)
                     /locus_tag="VFY44_10840"
     CDS             complement(1399..2286)
                     /locus_tag="VFY44_10840"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5910983.1"
                     /translation="MTAPPDVPGARHTTVSVATADAGAIDLHVLEAGEPGKPPVLLLH
                     GYPQHAWCWRHVIPLLAPHHRLIAPDLRGFGWSDCPEGGYDPVTFAADAVALLDALEI
                     EQADVVGHDWGGSTAFALALTAPERVRRMVILNTIAPWVRRSPRLIAQARRSWYTVAM
                     ALVGDVVLRRRPQMVAENIRKDAVHPSGMTEEDAMAYALRLGEPARARASKLLYRTYL
                     KTAVGRAPGPDLSERRLTQPTRFLFGVHDAYVAPVTLEGVEEHGDDLTLEMVEDSGHF
                     IAEEKPELVARRALDLFGA"
BASE COUNT          294 a          915 c          927 g          305 t
ORIGIN      
        1 caacgtcaat cgcatgtgcg ccggcgtcct ccaggtggtg gggggtgcgg tcgagctggt
       61 gctggacggg cggccgcgcc gcgtcgcggc cggccagtac gccgaggtcc cgcgcgacgt
      121 gccgtggcgg gccgtggtcg aggccgacgc gcacgtgttg tggttcggcg ccccggcggg
      181 catcgagagc ctggtgggca tgctcggcca cccgccgctg gacgacgacg acgcggccgc
      241 cctgctggcc gccgcgggcg tcagccgcgt gcgcgcgtca tggtgaggtg cccggcggcg
      301 tgagccgcgc ggcctgggtg tccaggccgg tgccgcgcag caggtcggtg gccgtggccc
      361 tgacctgtcc caccaccatg ttcgtgcgca ggtcgcttcg ccggcccagc accgaggtcg
      421 cctggtcggc ggcctggcgg gccaggtagc gcgtctcctc gtcctcctgg tccggggtgg
      481 ccagctcacg gcccagcgcg cgcacggcgt cggccaggat ggacaccgcg tcggccagct
      541 cctggtccgg gggcccggtg cgggccgcct gggcggcgtt gcgggccagg atgcgggtgt
      601 tgcgcaccgc gtagtccacc tgccgcgcgg cgtcggcgta cgagcgcagg tggcccagcg
      661 agcggcgccg gcgcgcgggc gagagccagg cgttctcgcg ccccacggcg acggcgtcgt
      721 agaaggcgcc gatcgcgtcg tccagcgccc gcacccgctc cagcgcgtgc tcggcggcgg
      781 tctggtcggc gtcggccagg gcgacggcgg tctcctcgag cgcaagcgcc agctcgtcca
      841 ggacccggcc cgccgccttg cccatctggc gcaccgggtc gcgcgggaac agcacctggc
      901 tcagcaccag cgccaccgcg gcgccgatca gcgcgtcgaa cagccggtcc agcgtgccgc
      961 cctggctcag gctcggcagc gtgatcacca ggatcgccga gatggcggcc tggttgacca
     1021 gcaggatccc ggcgccgacc accagcgcgg cgctcatggc cagcaccacc accacgccga
     1081 tctgccaggt gccggtgccg atcagctgca ccagcagatc ggcgatcagg atgcccagcg
     1141 cgacgccgag gcccagctcc accgcgcgcc ggcgtggctg tccgcgtgac agccccaggg
     1201 ccagcaccgc ggcgatgggg gcgaagaacg gctggctctg gcccacgatc agcttggccg
     1261 cctgccacgc gaccgcggcg gcgacgccgg tctggaggat ctccagccac gcacccgtga
     1321 ccctgtccat gcgccgccgc gccgacgagc gcaccgccgc gacggcgtgg gcctcgcgca
     1381 gacggtcgga cctgtcgctc acgccccgaa gaggtcgagc gcccggcggg ccaccagctc
     1441 cggcttctcc tcggcgatga agtgccccga gtcctcgacc atctccagcg tcaggtcgtc
     1501 gccgtgctcc tccacgccct ccagggtcac gggggccacg taggcgtcgt ggacgccgaa
     1561 caggaagcgg gtgggctggg tcaggcgccg ttcggacagg tccggccccg gcgcgcggcc
     1621 gacggcggtc ttcaggtagg tgcggtacag cagcttcgac gcccgggcgc gggcgggctc
     1681 gcccaggcgc agcgcgtagg ccatcgcgtc ctcctcggtc atcccggacg ggtggaccgc
     1741 gtccttgcgg atgttctcgg ccaccatctg cgggcggcgg cgcagcacca cgtcgccgac
     1801 gagcgccatg gccacggtgt accagctgcg ccgggcctgg gcgatcaggc gcggtgagcg
     1861 ccgcacccag ggcgcgatgg tgttcaggat caccatccgc cgcacccgct cgggcgcggt
     1921 cagcgccagc gcgaaggcgg tgctgccgcc ccagtcgtgg ccgaccacgt ccgcctgctc
     1981 gatctccagc gcgtccagca gcgcgaccgc gtcggcggcg aacgtgaccg ggtcgtagcc
     2041 gccctccggg cagtcgctcc agccgaagcc gcgcaggtcg ggcgcgatca ggcggtggtg
     2101 tggagccagc agcgggatca cgtggcgcca gcaccaggcg tgctgcgggt agccgtgcag
     2161 cagcagcacg ggcggcttcc cgggctcgcc ggcctccagc acgtgcaggt cgatggcgcc
     2221 cgcgtcggcg gtcgcgaccg acacggtggt gtgccgggct ccggggacgt cgggcggagc
     2281 ggtcacgggg ctcgcctacc ccttcttggt ggccacgaag ctcagggcca cctcgccccg
     2341 caccaggagg cggtacatgc ggtaggcgcg gcggccggcc cggctgcgct gcagcaggcg
     2401 gatgccctgg accttgagcg cgtcccaccg ccgcgccgac g
//
LOCUS       DASUPW010000485         3233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1913749, whole genome shotgun sequence.
ACCESSION   DASUPW010000485 DASUPW010000000
VERSION     DASUPW010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1913749"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VFY44_10845"
     CDS             complement(<1..416)
                     /locus_tag="VFY44_10845"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEX5910984.1"
                     /translation="MSQDTTRAAAFFDLDKTLIEGSSAVHFARAAYRSGMRSRREIAG
                     DLYANLKFRFRGSTDEETDALRDAVLQALAGQREVDLQRLGPGVLAGILPLIYTEVLE
                     EAWSHIDAGRPAYIVTAASHELAETLASVLALTGGIG"
     gene            complement(413..952)
                     /locus_tag="VFY44_10850"
     CDS             complement(413..952)
                     /locus_tag="VFY44_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910985.1"
                     /translation="MRRALALAVVAMAVAAPAASAQAPDYTIRAKGSKTGLGKVSAIG
                     PFKLAGDPNLTGATAVFGTQSSLQKTATLCRPGWRAIGLRISFVNLGGGDSCQLGSVQ
                     TVSAFGKVWRTERGLRIGSPVKALRRLYPGALRKGRTYRLVGAKSIFTGGSRYSVLAA
                     RTDGKRVKSFRLFVGAAGE"
     gene            complement(952..2136)
                     /locus_tag="VFY44_10855"
     CDS             complement(952..2136)
                     /locus_tag="VFY44_10855"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX5910986.1"
                     /translation="MPKTVILGTARTPFGKMGGALASQDATDLGGHAIEHALERSEVK
                     PDEVQHVVFGQVLQAGQGQVPSRQAQIKGGIPKEVSSETVNKVCASGIRAAGMVDQAV
                     RAGDLDVAVAGGMESMSKAPYMLPKARFGYRMGDAKMLDAMVHDGLTNPFTGKQMFVE
                     ATEVGDELEITRADMDKFALQSHDRAIAATDDGRLAEEIAPVTISSKKGDTVVEVDEP
                     PRRGSSLESLAKLPGLTGKDGSHTAGNAPGVNDGAGALVLSSDEWAKANGKTPLATII
                     GQAAVADDFPYLARTPANATKKALEQAGLSIDDIDLFEINEAFASVAINSLRMLGVEE
                     DKVNVNGGAIAMGHPIGASGARILGALVHELRRRGGGLGVAAICSGGGQGDAVILEVA
                     GG"
     gene            complement(2226..>3233)
                     /locus_tag="VFY44_10860"
     CDS             complement(2226..>3233)
                     /locus_tag="VFY44_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910987.1"
                     /translation="KIGGSPLDMATDGAGNVWATDANGVVRINTAPAVPTFDDVTVLG
                     ASGREIALGGDGRMWWADFGGSAIQAVTTGAVPANAKVIDTTAAPQGIAAGPGTQMAF
                     GAPNNLLGRVGPAGGAQYTTDTGGDAGFGIVLGQDGAYWAPRFPKDSVGRLTPDGGYT
                     TPIALPAGSGPRRIAAGSGNTLWVTLELSKQIARISGVTPPPAANPGGGGTAKDTVKP
                     RLARLKVNAAKRRFTVRLSEAASLRVTIEKRTFGKRKGKKCRAAKKGRTGKKCVRYVK
                     VRSFRKAGTAGDNTVSMGRKLAPASYRLSLAAVDAAGNRSATARKGFTVKKPKKARKR
                     K"
BASE COUNT          379 a         1163 c         1189 g          502 t
ORIGIN      
        1 ccgatgccgc cggtcagcgc caggacggag gccagcgtct cggccagctc gtggctggcg
       61 gccgtgacga tgtaggcggg ccggccggcg tcgatgtggc tccacgcctc ctccagcacc
      121 tccgtgtaga tcagcggcag gatcccggcc agcacgcccg gccccaggcg ctggagatcg
      181 acctcgcgct ggcccgccag ggcctgcagc acggcgtcgc gcagcgcgtc ggtctcctcg
      241 tcggtggagc cgcggaagcg gaacttgagg ttggcgtaca ggtcgccggc gatctcgcgc
      301 cgcgagcgca tgcccgagcg gtaggcggcc cgggcgaagt gcaccgcgga cgacccctcg
      361 atcagggtct tgtccaggtc gaagaacgct gcggcgcggg tcgtgtcctg gctcattcgc
      421 cggcggcgcc gacgaacagc cggaacgact tgacccgctt gccgtcggtc cgggccgcca
      481 gcacggagta gcggctgccg ccggtgaaga tgctcttcgc gccgaccagc cggtaggtgc
      541 gccccttgcg cagcgcgccg gggtagagcc tgcgcagcgc cttgaccggc gatccgatcc
      601 tgagcccgcg ctcggtccgc cagaccttgc cgaacgcgga cacggtctgg acgctgccca
      661 gctggcacga gtcgccgccg cccaggttca cgaagctgat ccgcaggccg atggcacgcc
      721 agcccggccg gcacagcgtg gcggtcttct ggaggctgga ctgggtgccg aagacggccg
      781 tggctcccgt caggttcggg tcgccggcca gcttgaacgg cccgatggcg ctgaccttgc
      841 ccagcccggt cttgctgccc ttggcccgga tcgtgtagtc gggcgcctgc gcggaggccg
      901 ccggagcggc caccgccatc gccacgacgg cgagtgcgag cgcccggcgc atcagccgcc
      961 cgcgacctcc aggatcaccg cgtcgccctg gccaccgccg gagcagatgg cggccacgcc
     1021 gagcccgccg ccgcggcggc gcagctcgtg caccagcgcg cccaggatgc gggcgcccga
     1081 ggcgccgatc gggtggccca tggcgatcgc gccgccgttg acgttgacct tgtcctcctc
     1141 gacccccagc atgcgcaggg agttgatggc caccgaggcg aacgcctcgt tgatctcgaa
     1201 caggtcgatg tcgtcgatcg acaggccggc ctgctcgagc gccttcttgg tggcgttggc
     1261 gggggtccgg gccaggtagg ggaagtcgtc ggcgaccgcc gcctggccga tgatcgtggc
     1321 cagcggggtc ttgccgttgg ccttggccca ctcgtcgctg gacagcacca gggcgccggc
     1381 gccgtcgttc acgcccgggg cgttgccggc ggtgtgcgag ccgtccttgc cggtcagccc
     1441 gggcagcttg gccagcgact ccagcgagga gccgcggcgc ggcggctcgt ccacctcgac
     1501 cacggtgtcg cccttcttcg agctgatcgt gaccggggcg atctcctcgg ccaggcggcc
     1561 gtcgtcggtc gcggcgatgg cgcggtcgtg gctctggagg gcgaacttgt ccatgtccgc
     1621 gcgcgtgatc tcgagctcgt cgccgacctc ggtggcctcg acgaacatct gcttgccggt
     1681 gaaggggttg gtcagcccgt cgtggaccat cgcgtccagc atcttggcgt cgcccatgcg
     1741 gtagccgaag cgggccttgg gcagcatgta cggggccttg gacatcgact ccatgccgcc
     1801 ggccacggcc acgtccaggt caccggcgcg gacggcctgg tccaccatgc ccgcggcgcg
     1861 gatgcccgag gcgcagacct tgttcacggt ctccgaggag acctccttcg ggatcccgcc
     1921 cttgatctgc gcctggcgcg agggcacctg gccctggccg gcctggagga cctggccgaa
     1981 gacgacgtgc tggacctcgt ccggcttgac ctcggaccgc tcgagcgcgt gctcgatggc
     2041 gtggccgccc aggtcggtgg cgtcctgcga ggccagtgct ccgcccatct tcccgaaggg
     2101 ggtgcgggcg gtgccgagga tcacggtctt gggcatgggg gcttgcggct ccttcgaagg
     2161 ggtcgaaacg gggccgcaga cgttaccggg gccgggggga actctgcccc cggccccggt
     2221 ggccgctact tgcgcttgcg ggccttctta ggcttcttga ccgtgaagcc cttcctggcc
     2281 gtggccgagc ggttgccggc cgcgtccacc gcagccagcg agaggcggta gctcgcgggc
     2341 gcgagcttcc tgcccatgct caccgtgttg tcaccggccg tgcccgcctt gcggaaggag
     2401 cgcaccttca cgtagcgcac gcacttcttg cccgtgcgcc ccttcttggc ggcgcggcac
     2461 ttcttgccct tgcgcttgcc gaaggtgcgc ttctcgatcg tcacccgcag cgaggcggcc
     2521 tcgctcaggc gcaccgtgaa ccggcgcttg gcggcgttga ccttgagcct cgccaggcgc
     2581 ggcttgaccg tgtccttggc ggtgccgccg ccgcccgggt tcgcggccgg cggcggggtc
     2641 actccgctga tgcgggcgat ctgcttcgag agctcgagcg tcacccacag cgtgttgccg
     2701 gagccggcgg cgatgcggcg cggccccgag ccggccggca gcgcgatcgg cgtggtgtac
     2761 ccaccgtccg gtgtcagccg gccgacgctg tccttgggga agcgcggcgc ccagtaggcg
     2821 ccgtcctggc cgagcacgat cccgaacccg gcgtcgccgc cggtgtcggt ggtgtactgg
     2881 gcgccgccgg cggggccgac ccggcccagc aggttgttcg gggcgccgaa ggccatctgg
     2941 gtgccggggc ccgcggcgat gccctgcggc gccgcggtgg tgtcgatgac cttggcgttc
     3001 gccggcaccg cgcccgtggt gacggcctgg atggcgctgc cgccgaagtc ggcccaccac
     3061 atgcggccat cgccgcccag tgcgatctcg cgcccgctgg cgcccaggac cgtgacgtcg
     3121 tcgaaggtgg gaacggcggg ggcggtgttg atcctgacca ccccgttggc gtcggtcgcc
     3181 cacacgttgc cggcaccgtc ggtggccatg tccagcgggc tgccgccgat ctt
//
LOCUS       DASUPW010000486         2047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_666078, whole genome shotgun sequence.
ACCESSION   DASUPW010000486 DASUPW010000000
VERSION     DASUPW010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666078"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1499)
                     /locus_tag="VFY44_10865"
     CDS             complement(<1..1499)
                     /locus_tag="VFY44_10865"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5910988.1"
                     /translation="MSWQRRGSRLWLMVVAGVLCAALGAVLQERDALDSAEMDTVDLR
                     FQLRGDQAKPKDIVLVAIDGQSLQDLNRLLPFPRRLHARAIDRLRRAGARQIVYDIQF
                     TEETTVADDNALFEAVRRAGTVVLATSEVRRDGGTDVLGGPAGQRAARASVGNAALSK
                     ADSDRVIRRYARATDGLESLAVVAARELGAKPARGPFRPDGAWIDYPGPPGTIDTVRF
                     SDLLAGRVPASRLRGKVAVVAVTDPNEKDVYGTPGSGAEVMAGGEIQAHALDTIRRDF
                     PLRPLPGGWAALFAALFGLAMPMAALKVRRWPWLAPVAAVVLAAIAYLAFLAGVIVPV
                     AAPLAALVVGTLATLAVDLATGSRARERMRGAFARFVPAQIVDDVIERTGDDYRLTGD
                     RLEATVLFCDLRGFTTLAESLDAERVIEVLDRYLTEMSDAILDQDGTVVSYMGDGIMA
                     VFGAPIADDDHAAKAVAAAREMIGPRLEAFNAWLAAEGVAPGGVRMGIGL"
     gene            1580..>2047
                     /locus_tag="VFY44_10870"
     CDS             1580..>2047
                     /locus_tag="VFY44_10870"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910989.1"
                     /translation="MIDRLRTRTALTAAAAFAALGGVALAAPASISTVAGTGTAGFAG
                     DAGPATLAQLNQPRDLARLPDGDVLVADTANNRIRRIDENGTITTFAGGNTPSPACGA
                     PRAAIALNAPEGVTVAPSGGVVVANTGGSQLVVFDAGGTVVGLVNGTFVGSSPA"
BASE COUNT          274 a          786 c          734 g          253 t
ORIGIN      
        1 aggccgatgc ccatgcgcac gccgccgggg gccacgccct ccgcggccag ccaggcgttg
       61 aacgcctcca gacggggccc gatcatctcg cgcgcggccg ccaccgcctt ggccgcgtgg
      121 tcgtcgtcgg cgatcggggc gccgaacacg gccatgatcc cgtcgcccat gtaggagacc
      181 acggtgccgt cctggtccag gatggcgtcg ctcatctcgg tcaggtagcg gtccagcacc
      241 tcgatcaccc gttccgcgtc cagcgactcg gccagggtgg tgaagccgcg caggtcgcag
      301 aacagcaccg tggcctccag ccggtcgccc gtcaggcggt agtcgtcgcc ggtgcgctcg
      361 atcacgtcgt ccacgatctg ggcgggcacg aaccgggcga acgccccgcg catgcgctcg
      421 cgcgcgcggg accctgtggc caggtccacg gccagcgtcg ccagcgtccc caccaccagc
      481 gcggccagcg gcgcggcgac gggcacgatc accccggcca ggaacgccag gtaggcgatg
      541 gccgccagca ccaccgcggc gaccggtgcc agccacggcc agcgccgcac cttcagcgcg
      601 gccatcggca tggccaggcc gaagagcgcc gcgaacagcg cggcccagcc gcccgggagg
      661 gggcgcaggg gaaagtcgcg ccggatggtg tccagggcgt gggcctggat ctcgccgccg
      721 gccatgacct cggctcccga gccgggcgtg ccgtagacgt ccttctcgtt ggggtccgtc
      781 accgcgacca ccgccacctt cccgcgcagg cgcgaggccg gcacgcggcc ggccaggagg
      841 tcggagaagc ggacggtgtc gatcgtgccc ggaggcccgg ggtagtcgat ccaggccccg
      901 tcggggcgaa acggcccgcg cgcgggcttg gcgccgagct cgcgggccgc caccaccgcc
      961 aggctctcga gcccgtcggt ggcgcgcgcg tagcggcgga tcacccggtc cgaatcggcc
     1021 ttcgacagcg ccgcgttgcc caccgaggcg cgggccgcgc gctgacccgc gggcccgcca
     1081 agcacgtcgg tgccgccgtc ccggcgcacc tcggaggttg ccagcaccac ggtccccgct
     1141 cgccgcacgg cttcgaacag cgcgttgtcg tcggccaccg tggtctcctc ggtgaactgg
     1201 atgtcgtaga caatctgtcg cgcgcccgcg cggcgcagcc ggtcgatggc gcgggcgtgc
     1261 aggcggcggg ggaagggcag cagcctgttc aggtcctgca ggctctggcc gtcgatggcc
     1321 accagcacga tgtccttcgg cttcgcctgg tcgccgcgca gctggaagcg caggtcgacc
     1381 gtgtccatct cggccgagtc cagcgcgtcg cgttcctgga gcacggcgcc gagcgccgcg
     1441 cacaggacgc cggccaccac catcagccac agccgcgagc cgcggcgctg ccaggacacc
     1501 agggcagcct aatgccgcat cgatctctgt aaacggggtt acacagatgt ccgctggacg
     1561 ggcgtaggtt gtgccaacga tgatcgacag gctccgcacc cgcaccgcac tcaccgccgc
     1621 cgcggccttc gcggcgctcg gcggtgtggc cctggccgcg ccggcctcga tctcgaccgt
     1681 cgcgggtacc ggcaccgcgg ggttcgccgg cgacgccggc ccggccaccc tggcccagct
     1741 caaccagccc cgtgacctgg cccggctgcc cgacggcgac gtgctggtgg ccgacaccgc
     1801 caacaaccgc atccgccgga tcgacgagaa cgggaccatc acgacgttcg ccggcgggaa
     1861 cacgccctcg ccggcctgcg gcgccccgcg cgccgcgatc gcgctcaacg cgcccgaggg
     1921 tgtgacggtg gcgccgtcgg ggggcgtcgt tgtggccaac accgggggct cccagctcgt
     1981 cgtcttcgat gccgggggca cggtcgtcgg cctggtgaac ggcacgttcg tgggctcgtc
     2041 cccggct
//
LOCUS       DASUPW010000487         3962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_943482, whole genome shotgun sequence.
ACCESSION   DASUPW010000487 DASUPW010000000
VERSION     DASUPW010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943482"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..1420
                     /locus_tag="VFY44_10875"
     CDS             221..1420
                     /locus_tag="VFY44_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910990.1"
                     /translation="MKILVTSARMPFALAIIRRLARSGHEVHASDTYSTAPGSHSRYV
                     TGHLVTSAPKFSTERFVHEVADYTREHGIDLIVPAWEDVFYLACHREIVESGGATLYA
                     GTFEALARLHDKHSFEVMVEQLGIRAPVGVTVYDDQQLHEAVGRWPHYFGRGVFSRGG
                     VTLLTNTGPLAGRVDIDDIHPTDDQPWLVQEFVDGPMVCTYSTLHEGRVTAHCMYRAP
                     RQFHHSTGIQFESIDGTESLKAVQKLGEELNYTGQMSLDFVDAEDGLVIIECNPRPTD
                     GTLLMEDEELAGGITDPSRELEMVPPGRETQLDFAVLAGIFDDGLKGVPGTIHDLVKV
                     RGSDRGWHDQLPNLYSFLAFCHHERLSLKEHEKLQVAMYEDVSWDGEPIEGMTAEQSA
                     AVEALRK"
     gene            1430..1906
                     /locus_tag="VFY44_10880"
     CDS             1430..1906
                     /locus_tag="VFY44_10880"
                     /inference="COORDINATES: protein motif:HMM:NF025196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4188 domain-containing protein"
                     /protein_id="HEX5910991.1"
                     /translation="MGPRVHAGRFTADIEGDFVVFLIGMRINKPWRVHEWWRVFVAMP
                     LMLRELDAAPNPPLLGWRLLLASPLSPTVIQYWRSYDDLEAYANDPQRRHKPAWQRFF
                     RDIGLNGNVGIWHETYTVRAGEYEAIYANMPRTGLAAAGRMRGLGSSSTSRERIAR"
     gene            1923..2876
                     /locus_tag="VFY44_10885"
     CDS             1923..2876
                     /locus_tag="VFY44_10885"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5910992.1"
                     /translation="MSDRLIDSALDRTIAPGYSRFGLAVRRRLGSWPADPPRMDGKVV
                     LVTGASSGLGRASAVGLARLGADVRVLTRDSDRSAKLARELEKETGGRVSGVTCDVSD
                     LTSVRRFTGRFAAAEDRLDVLINNAGVMPPERTETADGIELTLATHVVGPHLLIERLS
                     PLLEAAAPSRVINVSSGGMYSQQLDLDDLQTEHETYRKNRVYAKTKRAQVVLTHEWAR
                     RLEPSGVAVQSMHPGWADTQGVRDSLPGFSKITRGIIRDAEQGADTIVWLAASPDAQR
                     PTGAFWHDRRPRPEHYLPGRGESDQDRRRLWDAVQELAGAV"
     gene            2886..3446
                     /locus_tag="VFY44_10890"
     CDS             2886..3446
                     /locus_tag="VFY44_10890"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5910993.1"
                     /translation="MRKLQSQGVHHITLVGADRRTSIDFWEGVLGMPFVFEQPNLDNA
                     SESHLYFDPGDGRLITIFTDEERAGDGERTPTDPGCVHHVAFAVSAATFAQAVERLDE
                     RGIEHSEVKDRGFMDSIYFKDPLGLLIELASYRFVPPHGFTHADVLLAAHRIRVQRDD
                     YNIAPVHLADAIEQLSSESRESLSDD"
     gene            3571..3768
                     /locus_tag="VFY44_10895"
     CDS             3571..3768
                     /locus_tag="VFY44_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910994.1"
                     /translation="MSQLASPRYTRQECLNCGAHTCGPKCGCCGNNHLDPIAGAPAIV
                     PVSDRELFTRPAPLPFTRWAP"
BASE COUNT          592 a         1443 c         1358 g          569 t
ORIGIN      
        1 cggtggctct ggccggtggg ccagctcctc ggttcgcgag tcgaccccac cttcctccgc
       61 cttgagcgtt ttggcacgac tcttgcgcgg ttgcccgcga atgtggcgaa aggcccgcaa
      121 aaggctgtgc gcggcgtgga ggttcgggcc cgggatccgt gcgcgcgcct ggacgcgtgt
      181 tcgggtagga gatcgccgcg agcccccgta gcctggggca atgaagatcc tcgtgaccag
      241 cgcccggatg ccctttgccc tcgcgatcat ccgccggctg gcgcgctccg ggcacgaggt
      301 ccacgcctcg gacacctact ccacggcgcc cggcagccac tcgcgctacg tcacggggca
      361 cctggtcacg tcggcgccca agttctcgac cgagcggttc gtgcacgagg tggccgacta
      421 cacgcgcgag cacggcatcg acctgatcgt gcccgcgtgg gaggacgtct tctacctggc
      481 ctgccaccgc gagatcgtgg agtccggtgg cgcgacgctg tacgccggca ccttcgaggc
      541 cctggcgcgc ctgcacgaca agcactcgtt cgaggtcatg gtcgagcagc tggggatccg
      601 cgcccccgtg ggcgtcacgg tctacgacga ccagcagctg cacgaggccg tcggccgctg
      661 gccgcactac ttcggccgcg gcgtgttctc acgcggcggc gtgacgctgc tgaccaacac
      721 cgggccgctc gcgggccgcg tggacatcga cgacatccac ccgaccgacg accagccgtg
      781 gctggtccag gagttcgtgg acgggccgat ggtgtgcacc tactccacgc tgcacgaggg
      841 acgcgtgacc gcgcactgca tgtaccgcgc gccgcgccag ttccaccact ccaccgggat
      901 ccagttcgag tcgatcgacg gcaccgagtc gctgaaggcg gtgcagaagc tgggggagga
      961 gctgaactac accggccaga tgtcgctgga cttcgtcgac gccgaggacg gcctggtcat
     1021 catcgagtgc aacccgcgcc ccaccgacgg caccctgctg atggaggacg aggagctggc
     1081 cggcggcatc accgacccgt cccgcgagct ggagatggtt ccgcccggcc gcgagaccca
     1141 gctggacttc gccgtgctgg ccggcatctt cgacgacggg ctcaagggcg tgcccggcac
     1201 catccacgac ctggtcaagg tgcgcggctc ggaccgcggc tggcacgacc agttgcccaa
     1261 cctgtactcg ttcctggcct tctgccacca cgagcggctc agcctcaagg agcacgagaa
     1321 gctccaggtg gccatgtacg aggacgtctc gtgggacggc gagccgatcg agggcatgac
     1381 cgccgagcaa tcggcggcag tggaagccct gcgcaagtag ggtcggcaca tgggcccgcg
     1441 cgttcacgcc ggccgcttca ccgccgacat cgaaggcgac ttcgtcgtgt tcctgatcgg
     1501 catgcgcatc aacaagccgt ggcgggtgca cgagtggtgg cgcgtgttcg tggccatgcc
     1561 gctgatgctg cgcgagctgg acgccgcgcc caacccgccg ctgctgggct ggcgcctgct
     1621 gctggcgtcg cccctgtcac ccacggtcat ccagtactgg cgcagctacg acgacctcga
     1681 ggcctacgcc aacgaccccc agcgccggca caaacccgcc tggcagcgct tcttccgcga
     1741 catcgggctg aacggcaacg tgggcatctg gcacgagacc tacacggtgc gcgcgggcga
     1801 gtacgaggcc atctacgcga acatgccccg gacgggcctg gcggcggccg gccggatgcg
     1861 cgggctgggc tccagctcga cctcgcgtga gcggatcgcc cgctgaggcc cgtatccccg
     1921 gcgtgagcga ccgcctgatc gacagcgccc tggaccgcac catcgccccc ggctacagcc
     1981 gcttcggcct cgccgtgcga cgacgcctgg gctcgtggcc cgccgaccca ccgcggatgg
     2041 acggcaaggt ggtgctggtc accggcgcgt cgtccggcct cggccgggcc tccgccgtcg
     2101 ggctggcccg gctgggggcc gacgtgcggg tgctcacccg cgacagcgac cgcagcgcca
     2161 agctggcgcg cgagctggag aaggagaccg gcggccgggt cagcggcgtc acctgcgacg
     2221 tcagcgacct gacgtcggtg cgccgcttca ccggccggtt cgcggccgcc gaggaccgtc
     2281 tggacgtcct gatcaacaac gccggcgtga tgccgcccga gcgcaccgag accgccgacg
     2341 ggatcgagct caccctggcc acccacgtcg tcggcccgca cctgctgatc gagcggctgt
     2401 cgccgctgct cgaggccgcc gcgccctcac gcgtcatcaa cgtgtcgtcc ggaggcatgt
     2461 actcccagca gctggacctc gacgacctcc agaccgagca cgagacctac cgcaagaacc
     2521 gggtctacgc gaagaccaag cgcgcgcagg tcgtgctgac ccacgagtgg gcgcgccggc
     2581 tcgagcccag cggggtcgcc gtccagtcga tgcacccggg ctgggccgac acccagggcg
     2641 tgcgcgactc gctgcccggg ttcagcaaga tcacccgcgg gatcatccgc gacgccgagc
     2701 agggcgccga caccatcgtc tggctggccg cgtcacccga cgcccagcgg ccgaccggcg
     2761 cgttctggca cgaccgccgc ccgcggcccg agcactacct gcccgggagg ggcgagtccg
     2821 accaggatcg gcgccgcctg tgggacgccg tgcaggagct ggccggagcg gtttagcatc
     2881 gcgccatgcg caagctccag agccagggcg tccaccacat caccctcgtc ggcgccgacc
     2941 gccggacgtc gatcgacttc tgggagggcg tgctggggat gccgttcgtc ttcgagcagc
     3001 cgaacctgga caacgcctcc gagagccacc tctacttcga tcccggcgac ggccggctga
     3061 tcacgatctt caccgacgag gagcgggccg gtgacggcga gcggacgccg accgaccccg
     3121 gctgcgtcca ccacgtggcc ttcgccgtct cggccgccac cttcgcccag gccgtcgagc
     3181 gcctggacga gcgcggcatc gagcacagcg aggtcaagga ccgcggcttc atggactcga
     3241 tctacttcaa ggaccccctg ggcctgctga tcgagctggc gtcctaccgc ttcgtcccgc
     3301 cccacggctt cacgcacgcc gacgtcctgc tggcggcgca ccgcatccgc gtgcagcgcg
     3361 acgactacaa catcgccccg gtccacctgg ccgacgcgat cgagcagctc agctccgagt
     3421 cgcgcgagtc gctctcggac gactagttca tgccgggcgc cgccggtgcc gataggaaca
     3481 agagcaccgc gccagagggt tcttcgctcg tcggcttcca ccctttcgtg gccccagttc
     3541 gatgaaggta cgacgagaga gcgccggacc atgtcgcagc tcgcctcacc gcggtacacc
     3601 cgtcaggagt gcctgaactg cggcgctcac acctgcggcc cgaagtgcgg ctgctgtggc
     3661 aacaaccacc tggacccgat cgccggggcc ccggcgatcg tcccggtctc cgaccgtgag
     3721 ctgttcacgc ggccggcgcc gctgccgttc acgcgctggg ctccgtgagg cctctccggc
     3781 cctgaccagc cgcggcgccg gtacggcggg cggtcgtcac taccttgggc tcaggctgct
     3841 cggccgcact tggacgaggg gttccgatag cgggggagga aattcgtgag gcgtcgttgg
     3901 gttggagtgc ttctggtggg cctggccgcg gtgggatgcg gtgacgggga gggtcggcaa
     3961 gg
//
LOCUS       DASUPW010000488         2322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_252836, whole genome shotgun sequence.
ACCESSION   DASUPW010000488 DASUPW010000000
VERSION     DASUPW010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252836"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VFY44_10900"
     CDS             complement(<1..264)
                     /locus_tag="VFY44_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5910995.1"
                     /translation="MRNRQLHDALRAFALEAAAMLRDDQAAGQELEFDLDEGHRRGGP
                     ALYHYRPLTSKYIADRWSRLRFLPSAAEACEALDTGASAYLRLN"
     gene            341..1171
                     /locus_tag="VFY44_10905"
     CDS             341..1171
                     /locus_tag="VFY44_10905"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEX5910996.1"
                     /translation="MTPELRASYAACRRVHRHHDPTYYWATRRLPADVRPAVHAVYAF
                     VRSADELVDGPGRASDPDARRAALDGLEDELREARAGGDTSDPTVAALADAGARHGLP
                     LEELDVYMRSMRIDCAPVRMTSRSELDGYMNGSAAAVGRIMAPLLGAGGSADSFAALG
                     QAFQLTNFIRDVREDFALDRIYLPGLDEAELAAGTASAGFKQALAEQVGHARRLFREG
                     APALGAVQPRVRPGMRMARAVYVRVLDRVERLDYDVLGRRAGLAPWEIAGAAVGSLRR
                     "
     gene            1168..2289
                     /locus_tag="VFY44_10910"
     CDS             1168..2289
                     /locus_tag="VFY44_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX5910997.1"
                     /translation="MTVRATLRGAERTPIERPRADVLICGASFAGLAVARELAGSGAD
                     VLIVDRYEVGERQTSACAAPTPWLEAMGVAGAIRQEIPHMAFHTPHGSARYRLPWSWS
                     SFDYRELCRLLFDQCDARFEIAKVEGRDERGHVVTDRGVLEAPEIVDALGWRRVLATS
                     GYQPPEAPLSRGLEVHPDGGGTDLDVWVDRSLVRRGYCWSVPADGEQRVGSGSYDPHQ
                     HVKEPTVELAGRLGVDAVRYQGNWFPHRLRDAAEAGVYFVGDSAGHCFPLSGEGIRTA
                     FYFGIACGRELRAVVEGARQPADALERYRRFSAGHGKAFSRALRLQSLIPALPPRVLA
                     LALKAIGAQPIVDRAFGWYLDQAHPAFASSPVPAVSRTS"
BASE COUNT          271 a          860 c          885 g          306 t
ORIGIN      
        1 gttcaggcgc aggtaggccg acgccccggt gtccagcgcc tcgcacgcct cggccgcgga
       61 cggcaggaag cgcagccggc tccagcggtc ggcgatgtac ttcgaggtca gcggccggta
      121 gtggtagagc gccggcccgc cgcggcggtg gccctcgtcg aggtcgaact cgagctcctg
      181 gccggcggcc tggtcgtcgc gcaacatggc cgcggcttcc agggcgaagg cgcggagcgc
      241 gtcgtggagc tggcggttgc gcatgaaccg ccgtttcgcc gcagcggtcg gcgatcctgc
      301 cagcatgaac cgcgtgcatg cgcctgtgcg tacgagggac atgacgccgg agctccgggc
      361 ctcctatgcc gcgtgccgcc gggtgcaccg gcaccacgac ccgacctact actgggccac
      421 ccggcgcctg ccggccgacg tccgccccgc cgtccacgcg gtgtacgcgt tcgtgcgctc
      481 ggccgacgag ctggtggacg gtcccggccg ggcctcggac cccgacgccc gccgcgccgc
      541 actggacggg ctggaggacg agctccgcga ggcccgggcg ggaggcgaca cctccgaccc
      601 cacggtggcc gccctggccg acgccggcgc ccggcacggg ctgccgctgg aggagctgga
      661 cgtgtacatg cgctcgatgc ggatcgactg cgccccggtg cggatgacct cacgctccga
      721 gctggacggc tacatgaacg gcagcgcggc cgccgtgggc cggatcatgg cgccgctgct
      781 gggagccggc gggtccgccg acagcttcgc cgccctgggc caggcgttcc agctgaccaa
      841 cttcatccgc gacgtgcgcg aggacttcgc cctggaccgg atctacctcc ccggcctgga
      901 cgaggccgag ctggccgccg gtaccgcgtc cgccggcttc aagcaggcgc tggccgagca
      961 ggtagggcac gcccgccggc tgttccgcga gggcgccccg gccctgggcg cggtgcagcc
     1021 gcgggtgcgc ccgggcatgc gcatggcccg cgcggtctac gtgcgcgtgc tggaccgcgt
     1081 cgagcggctg gactacgacg tgctgggccg gcgtgcgggg ctggcgccct gggagatcgc
     1141 gggcgccgcc gtgggatcgc tgcgccggtg acggtccggg cgaccctgcg cggcgccgag
     1201 cgcaccccga tcgagcgccc ccgggcggac gtcctgatct gcggcgccag cttcgccggc
     1261 ctggccgtgg cccgcgagct ggccgggtcc ggcgccgacg tcctgatcgt cgaccgctac
     1321 gaggtcggcg agcgccagac ctcggcctgc gccgccccca ccccgtggct ggaggcgatg
     1381 ggcgtggccg gcgccatccg ccaggagatc ccgcacatgg ccttccacac cccgcacggc
     1441 agcgcgcgct accggctgcc ctggagctgg tcctcgttcg actaccgcga gctgtgccgg
     1501 ctgttgttcg accagtgcga cgcgcgcttc gagatcgcca aggtcgaggg gcgcgacgag
     1561 cgcggccacg tggtcaccga ccgcggcgtg ctcgaggcgc cggagatcgt ggacgccctg
     1621 ggctggcggc gggtgctggc caccagcggc taccaacccc cggaggcgcc gctgtcgcgc
     1681 ggcctggagg tgcaccccga cggcggcggc accgacctgg acgtgtgggt ggaccggtcg
     1741 ctggtgcggc ggggctactg ctggagcgtc ccggccgacg gcgagcagcg ggtgggctcg
     1801 gggtcctacg acccccacca gcacgtcaag gagcccacgg tcgagctggc cgggcgcctg
     1861 ggcgtggacg ccgtgcgata ccagggcaac tggttcccgc accggctgcg cgacgcggcc
     1921 gaggccggcg tgtacttcgt cggcgactcg gccggccact gcttcccgct ttcgggtgag
     1981 ggcatccgca ccgcgttcta cttcggcatc gcctgcgggc gcgagctgcg ggccgtggtc
     2041 gagggcgcac gccagcccgc cgacgccctg gagcgctacc gccgcttcag cgccgggcac
     2101 gggaaggcgt tctcgcgcgc gctcaggctc cagagcctga tcccggcgct accgccccgc
     2161 gtgctggcgc tggcgctgaa ggcgatcggg gcacagccga tcgtggaccg cgcgttcggc
     2221 tggtatctgg accaggcgca tccggcgttc gcgtcgagtc ccgtgcctgc ggtcagccgc
     2281 accagctgat cgaccaacgg ccggctgggt agggcccgta tt
//
LOCUS       DASUPW010000489         4269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1637320, whole genome shotgun sequence.
ACCESSION   DASUPW010000489 DASUPW010000000
VERSION     DASUPW010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1637320"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1357)
                     /locus_tag="VFY44_10915"
     CDS             complement(<1..1357)
                     /locus_tag="VFY44_10915"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX5910998.1"
                     /translation="MLVLEILGVRLLAPYVGLTLQTTTAIIGAVLAGIAAGAALGGRA
                     ADLTDPRRLLAWLLAGGGILALLTVPVVRALGPALRGSGDAGALVVTFAALLPPAAVL
                     SAVTPTLARLQLRDLGSSGTVVGRLSAWATAGALAGTFVTGFVLVPRLPVTASVIAVG
                     VVVVAAGLLVGLRGAVLGATGAAIVVAAALAAGTLTAVASPPCDVESAYHCVRIDRDP
                     ERASGRVLVLDDLRHSYVDLADPGHLEFDYTRWIADAVDGSAPPGRPLDAVFVGGGGF
                     TLPRWLAATRPGSRSRVLEVDGDLVDVARERLALRTSPALRVQEGDARMTMRDRPPGS
                     ADVVVGDAFGGRAVPWHLATREWAEEVRRVLRPGGVYALNVIDQGGLRLMRAEAATLL
                     DVFADVRLVAKPGLAGGNLVLVASGRRLPAPVGSRARGARTHGRAAVARLAAGSDPLT
                     DD"
     gene            complement(1429..1698)
                     /locus_tag="VFY44_10920"
     CDS             complement(1429..1698)
                     /locus_tag="VFY44_10920"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEX5910999.1"
                     /translation="MCRNIRTLHNFEPPASQEEVEAAALQYVRKVSGSTKPSQANAEA
                     FDHAVEQVTAITRELLDRLVTSAPPKDREVEAAKAKARAAKRYAA"
     gene            1721..2230
                     /locus_tag="VFY44_10925"
     CDS             1721..2230
                     /locus_tag="VFY44_10925"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEX5911000.1"
                     /translation="MATHAAFLRGMNVGGHRITNDDLRAAFARIGFEDVGVFRASGNV
                     AFDGGRSDPTPKIEKGLEAELGYAVPTFVRTAEEMHALAEADPFDGAEGKVQVVFLRK
                     PPPKSVLKRATDTDRLALGPGAELFWLPSGGLMESDLGMDGLEDEVGPGTTRTKGTIE
                     QITKKFFAG"
     gene            complement(2227..3882)
                     /gene="ettA"
                     /locus_tag="VFY44_10930"
     CDS             complement(2227..3882)
                     /gene="ettA"
                     /locus_tag="VFY44_10930"
                     /inference="COORDINATES: protein motif:HMM:NF008775.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEX5911001.1"
                     /translation="MPEFIFTMRNLTKVHPPDKEVLKDVTLAFYPGAKIGVLGANGSG
                     KSSLLRIMAGLDTEVRGEADLADGASVGLLEQEPQLDETKDVRGNVEDGVRAMKDLID
                     RFNELAANYSEETADEFAKLQEKIDAADAWNLDTMLDTAMDALRCPPADADVSKLSGG
                     ERRRVALCRLLLSQPDLLLLDEPTNHLDAESVAWLERHLDEYPGAVVAVTHDRYFLDN
                     VAGWILELDRGRGLPFEGNYSSWLEQKQKRLAQEEKKESTRQRTLAAELEWVRMNASA
                     RLTKNKARLARYDALLAEERNAKVDSVEIHIPAGPRLGDVVVEAEHVSKGYGDRLLIE
                     NLNFKLPPAGIVGIIGGNGAGKTTLLRMITGEEQPDSGELRVGETVELSYVDQSRDAL
                     DPAKSVWEEISGGADQLKVGDREMNSRAYVSSFNFKGSDQQKAVGKLSGGERNRLHMA
                     KLLRSAGNVLLLDEPSNDLDVDTLRALEEAIQAFPGCVVVVSHDRWFLDRVATHVLAF
                     EGDSKVHFFEGNFEAYEQWKREQLGEEADRPHRMTYKKLVARS"
     gene            3910..>4269
                     /locus_tag="VFY44_10935"
     CDS             3910..>4269
                     /locus_tag="VFY44_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911002.1"
                     /translation="MASPKLTDRVERAIEDRTDEEELERAPAPVRRRQITGAFWLTVC
                     GVSLYLVGPTVLDTLDEADRVRGIAPGWLGAMAGLQLLSLACLWELQRIALHTRDMTS
                     VATSQLAGNALAKVAPGG"
BASE COUNT          575 a         1557 c         1541 g          596 t
ORIGIN      
        1 cgtcgtcggt gagcggatcg gagcccgcgg ccaggcgcgc cacggcggcg cggccgtggg
       61 tccgggcccc ccgcgcccgc gaccccaccg gggccggcag gcggcgcccg gaggccacca
      121 ggaccaggtt cccgccggcc aggccgggct tcgcgaccaa ccgcacgtcg gcgaacacgt
      181 ccagcagggt ggccgcctcg gcgcgcatca gccggagccc gccctggtcg atcacgttca
      241 gcgcgtagac gccgccgggg cgcagcaccc gccgcacctc ctcggcccac tccctcgtgg
      301 ccaggtgcca gggcacggcg cggccgccga aggcgtcgcc gaccaccacg tccgccgaac
      361 ccggcggccg gtcgcgcatg gtcatccggg cgtcgccctc ctgcacgcgc agcgcgggcg
      421 aggtgcgcag ggccagccgc tcccgggcca cgtcgaccag gtcgccgtcg acctccagca
      481 cgcgcgagcg cgagccgggc cgcgtggcgg cgagccagcg cggcagcgtg aagccgcccc
      541 cgccgacgaa cacggcgtcc agcgggcgcc cgggcggggc cgagccgtcc accgcatcgg
      601 cgatccagcg ggtgtagtcg aactccaggt gccctgggtc ggccaggtcc acgtacgagt
      661 ggcgcaggtc gtccagcacc aggacccggc ccgaagcccg ctcggggtcg cggtcgatgc
      721 ggacgcagtg gtacgcgctc tcgacgtcgc acggcggcga ggccacggcg gtcagcgtgc
      781 ccgccgccag ggcggcggcc accacgatcg ccgcgccggt ggctcccagc accgccccgc
      841 gcagcccgac cagcagcccg gcggccacca ccaccacacc tacggcgatc accgaggcgg
      901 tcaccggtag tcgcggcacc agcacgaacc cggtgacgaa ggtcccggcc agggcgcccg
      961 ccgtggccca ggccgacagg cgcccgacca cggtcccgct ggaccccagg tcgcgcagct
     1021 ggagccgggc cagcgtcggc gtgacggcgc tgagcaccgc ggccgggggc aggagcgcgg
     1081 cgaacgtgac caccagtgcg cccgcgtcgc cgctgccccg cagcgccggt cccagcgccc
     1141 gcaccacggg cacggtcagc agcgccagga tcccgccgcc cgccagcagc caggccagca
     1201 ggcggcgcgg atcggtcagg tcggccgctc ggccgcccag ggcggctccg gcggcgatgc
     1261 cggccagcac ggcgccgatg atggcggtgg tggtctggag cgtcaggccg acgtacggcg
     1321 ccagcagccg cacgcccagg atctcgagca ccagcacggc gcccgaggac aggaaggcca
     1381 gggccgcggc cgcctcccgg ggcagcggct gccggatctc gtcgtcgctc aggccgcgta
     1441 ccgcttcgcg gcccgcgcct tggccttggc cgcctcgacc tcgcggtcct tgggcggcgc
     1501 gctggtcacc agccggtcca gcagctcacg ggtgatggcc gtgacctgct cgaccgcgtg
     1561 gtcgaacgcc tcggcgttgg cctgtgaggg cttggtggac ccgctgacct tgcgcacgta
     1621 ctgcagcgcg gcggcctcga cctcttcctg gctggcgggt ggctcgaaat tgtggagtgt
     1681 tcggatgttc cggcacatgc ctggatcgta cgatgtcggc gtggccacac acgccgcatt
     1741 tctccggggc atgaacgtgg gcggccaccg gatcaccaac gacgacctgc gcgcggcgtt
     1801 cgcgcgcatc ggcttcgagg acgtcggtgt gttccgcgcc agcgggaacg tggccttcga
     1861 cggcggcagg agcgacccca cgccgaagat cgagaagggc ctggaggcag agctgggcta
     1921 cgccgtgccc acgttcgtgc gcacggcgga ggagatgcac gccctggcgg aggccgaccc
     1981 gttcgacggc gccgagggca aggtgcaggt ggtgttcctg cgcaagccgc caccgaagtc
     2041 ggtgctgaaa cgggccacgg acaccgaccg gctggcgctg ggtcccggcg ccgagctctt
     2101 ctggctcccg tccggtgggc tgatggagtc cgacctgggc atggacgggc tcgaggacga
     2161 ggtcgggccg ggcaccacgc gcaccaaggg cacgatcgag cagatcacga agaagttctt
     2221 cgcgggctag ctgcgcgcga cgagcttctt gtacgtcatc cggtgcgggc ggtcggcctc
     2281 ttcgccgagc tgctcgcgct tccactgctc gtaggcctcg aagttgccct cgaagaagtg
     2341 gaccttggag tcgccctcga aggccaggac gtgggtggcc acgcggtcca ggaaccagcg
     2401 gtcgtgcgag accaccacca cgcagcccgg gaaggcctgg atggcctcct ccagcgcgcg
     2461 cagggtgtcc acgtccaggt cgttggacgg ctcgtccagc agcagcacgt tgccggcgct
     2521 gcggagcagc ttggccatgt gcagccggtt gcgctcgccg ccggagagct tgccgacggc
     2581 cttctgctgg tccgagccct tgaagttgaa gctcgagacg taggcgcgtg agttcatctc
     2641 gcggtcgccg accttgagct ggtcggcccc gccggagatc tcctcccaca ccgacttggc
     2701 cgggtcgagc gcgtcgcggg actggtccac gtaggacagc tcgacggtct cccccacccg
     2761 cagctcgccg ctgtcgggct gctcctcgcc ggtgatcatg cgcagcagcg tggtcttgcc
     2821 ggcgccgttg ccgccgatga tgcccacgat cccggccggc ggcagcttga agttcaggtt
     2881 ctcgatcagc agccggtcgc cgtagccctt ggagacgtgc tcggcctcga ccacgacatc
     2941 gcccagccgg gggccggcgg ggatgtggat ctcgacgctg tcgaccttgg cgttgcgctc
     3001 ctcggccagc agcgcgtcgt aacgggccag ccgggccttg ttcttggtca ggcgcgccga
     3061 cgcgttcatg cgcacccatt cgagctctgc ggccagggtg cgctggcgcg tggactcctt
     3121 cttctcctcc tgcgccagcc gcttctgctt ctgctccagc caggacgagt agttgccctc
     3181 gaacggcagc ccgcggccgc ggtccagctc caggatccag ccggccacgt tgtccaggaa
     3241 gtagcggtcg tgggtcacgg ccaccacggc gcccgggtac tcgtccagat ggcgctccag
     3301 ccacgccacc gactcggcgt ccaggtggtt ggtgggctcg tccagcagga gcaggtcggg
     3361 ctggctcagc agcagccggc acagcgccac gcggcggcgc tcgccacctg agagcttgct
     3421 gacgtccgcg tcggccggcg ggcagcgcag agcgtccatc gcggtgtcca gcatggtgtc
     3481 caggttccac gcgtccgcgg cgtcgatctt ctcctggagc ttggcgaact cgtccgcggt
     3541 ctcctcggag tagttggccg ccagctcgtt gaaccggtcg atcaggtcct tcatggcccg
     3601 gaccccgtcc tcgacgttgc cgcggacgtc cttggtctcg tccagctggg gctcctgctc
     3661 cagcagcccc acgctggcgc cgtcggccag gtcggcctcg ccccggacct cggtgtccag
     3721 gccggccatg atccgcagca ggctggactt gcccgagccg ttggcgccca ggacgccgat
     3781 cttggcgccg ggatagaagg ccagggtcac gtccttgagg acttccttgt cgggcggatg
     3841 caccttggtc aggttgcgca tcgtgaagat gaactcgggc atggccgcta gtaaagcgac
     3901 aatgggcgcg tggcctcccc caagctgacg gatcgcgtcg agcgcgcgat cgaggacagg
     3961 accgacgagg aggagctcga gcgggctccc gcgccggtcc gcaggcgcca gatcaccggc
     4021 gcgttctggc tgacggtgtg cggcgtctcg ctctacctgg tggggccgac ggtgctggac
     4081 acgctggacg aggcggaccg ggtgcggggc atcgcccccg gctggctggg ggcgatggcc
     4141 ggcctccagc tcttgtccct ggcctgcctc tgggagctgc agcggattgc gctgcacacc
     4201 cgcgacatga cctcggtggc cacctcgcag ctggccggaa acgcgttggc caaggtggct
     4261 cccggaggc
//
LOCUS       DASUPW010000490         2909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1914825, whole genome shotgun sequence.
ACCESSION   DASUPW010000490 DASUPW010000000
VERSION     DASUPW010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1914825"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..383
                     /locus_tag="VFY44_10940"
     CDS             18..383
                     /locus_tag="VFY44_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911003.1"
                     /translation="MTAVGEELARAIAAKDAAALRELLRPDVDFTAITPRRHWNAGTP
                     DDVAEIVFGSWFEDKDRIDSIEHVETDAFADRERVGYRFAITNPEGRFLVEQQAYLEV
                     EDGRIGWMRLVCSGFRPVG"
     gene            complement(380..739)
                     /locus_tag="VFY44_10945"
     CDS             complement(380..739)
                     /locus_tag="VFY44_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HEX5911004.1"
                     /translation="MGVAVGLFSRKKDPKQELAEVIVNRGVRVRGTVESFAVDGATAT
                     MSVRFAPEGQGERTVTVVQDMAPQVQVGLEPGAPVELSYDRDDPSTVLIWGSPLYRTT
                     EEGAVVRAVDVQGGERS"
     gene            complement(730..2346)
                     /gene="guaA"
                     /locus_tag="VFY44_10950"
     CDS             complement(730..2346)
                     /gene="guaA"
                     /locus_tag="VFY44_10950"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX5911005.1"
                     /translation="MDRPAAISTVPADDAEAVEPQATAAAHDEVLVLDFGGQYSQLIA
                     RRVRECGVFSELLHHTVPIEEIKQRNPRGLILSGGPASVYADDAPVLRPELLKLGIPV
                     LGICYGMQAMVLNLGGKVEGAEVGEFGRSTLTVSEPGTLLAGLPEEQPCWMSHRDTVY
                     EAPPGMTALASSSESPVAAFEDTERQLYGIQFHPEVVHTPYGTQILTRFLKDIAGAGM
                     DWNAASVAEEQIRRIREQVGDGQVICGLSGGVDSSVAALLVHRAIGDQLTCVFVDHGL
                     MRKGEGDQVVAAFRDHFKVPLVAVDAEERFLAKLAGVTEPEAKRKIIGNEFIRVFEEE
                     AAKLEDPRYLVQGTLYSDVIESGGDGTGAATIKSHHNVGGLPEDLEFELVEPLRALFK
                     DEVRQVGLELGLPERLVWRQPFPGPGLGIRIVGGEVNRERLEILRDADYVLQDEIRGA
                     GLYRDLWQSFCVLPGDLRTVGVQGDERTYGYVVVIRAVTSDDAMTADWARLPYDLLER
                     TASRMINEIRQVNRVVLDVTSKPPGTIEWE"
     gene            complement(2347..>2909)
                     /locus_tag="VFY44_10955"
     CDS             complement(2347..>2909)
                     /locus_tag="VFY44_10955"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: protein motif:HMM:NF012690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX5911006.1"
                     /translation="IGVPQATAIADVAAARSQHMLETGEYVKVIADGGMRNGGDISKA
                     VACGADAVMIGSPLARAYEAPGHGYHWGMATFHPELPRGARVATTQNGTVEQILMGPA
                     HENDGTFNLMGSLRTSMATCGYEDIPSFHRAEVMVAPSLQTEGKQLQRDQAIGMGSQS
                     AAKAAPASDGVTNGGAPAQPEAALVE"
BASE COUNT          395 a         1042 c         1038 g          434 t
ORIGIN      
        1 cgagtcagac tcgagccatg accgccgtcg gggaggagct cgcccgcgcc atcgccgcca
       61 aggacgcggc cgcgctgcgc gagctgctgc gccccgacgt cgatttcacc gcgatcaccc
      121 cgcgccggca ctggaacgcc gggacgccgg acgatgtcgc cgagatcgtg ttcggctcgt
      181 ggttcgagga caaggaccgg atcgactcga tcgagcacgt ggagaccgac gcgttcgccg
      241 accgcgagcg cgtgggctac cggttcgcca tcaccaaccc cgaaggccgg ttcctggtcg
      301 agcagcaggc ctacctcgag gtcgaggacg ggcggatcgg ctggatgcgg ctggtgtgct
      361 cgggcttccg gcctgtgggc taggagcgct cgccgccctg gacgtccacg gcccggacca
      421 ccgcgccctc ctcggtggtg cggtacagcg gcgagcccca gatgagcacg gtcgaggggt
      481 cgtcgcggtc ataggacagc tcaaccggcg cgccgggctc gaggcccacc tgcacctggg
      541 gcgccatgtc ctgcaccacg gtcacggtcc gctcgccctg gccctcggga gcgaaccgca
      601 cgctcatcgt cgcggtggct ccgtcgacgg cgaacgactc gaccgtgccg cgcacgcgca
      661 ctccgcggtt gacgatgacc tcggccagtt cctgcttggg gtccttcttg cgtgagaaca
      721 gccccacggc tactcccact cgatggtgcc cgggggtttg ctggtgacgt ccagcaccac
      781 ccggttgacc tggcggatct cgttgatcat ccgcgaggcc gtgcgctcca gcaggtcgta
      841 gggcagccgc gcccagtcgg ccgtcatcgc gtcgtcgctg gtcacggcgc ggatgacgac
      901 cacgtagccg taggtgcgct cgtcgccctg cacgccgacc gtgcgcaggt cgccgggcag
      961 cacgcagaag gactgccaca ggtcgcggta gaggccggcg ccgcggatct cgtcctggag
     1021 cacgtagtcg gcgtcgcgca ggatctccag gcgctcgcgg ttgacctcgc ctccgacgat
     1081 ccggatgccc agcccggggc cggggaaggg ctggcgccac accaggcgct cgggcaggcc
     1141 cagctccagg cccacctggc gcacctcgtc cttgaacagg gcgcgcagcg gctcgacgag
     1201 ctcgaactcc aggtcctcgg gcaggccgcc cacgttgtgg tggctcttga tcgtggccgc
     1261 gccggtgccg tcgcccccgg actcgatcac gtcggagtag agcgtgccct ggaccaggta
     1321 gcgggggtcc tcgagcttgg ccgcctcctc ctcgaacacg cggatgaact cgtttccgat
     1381 gatcttgcgc ttggcctcgg gctcggtgac gccggccagc ttggccagga accgctcctc
     1441 ggcgtccacg gccaccagcg gcaccttgaa gtggtcgcgg aaggcggcca cgacctggtc
     1501 gccctcgccc ttgcgcatca gcccgtggtc aacgaacacg caggtcagct ggtcgccgat
     1561 ggcgcggtgc accagcaggg cggccacgga cgagtcgacg cccccggaca ggccgcagat
     1621 gacctggccg tcgcccacct gctcgcggat gcggcggatc tgctcctcgg ccaccgacgc
     1681 ggcgttccag tccatgccgg cgccggcgat gtccttcagg aagcgggtca ggatctgggt
     1741 gccgtagggc gtgtggacga cctcggggtg gaactggatc ccgtacagct ggcgctcggt
     1801 gtcctcgaac gccgccacgg gcgactccga cgacgacgcc agcgcggtca tgccgggcgg
     1861 cgcctcgtag acggtgtcgc ggtggctcat ccagcacggc tgctcttccg gcaggccggc
     1921 cagcagggtg ccgggctcgg agaccgtgag cgtggagcgg ccgaactcgc cgacctcggc
     1981 gccctcgacc ttgccgccca ggttcagcac catggcctgc atcccgtaac agatgcccag
     2041 caccgggatc cccagcttga gcagctccgg gcgcaggacg ggcgcgtcgt cggcgtacac
     2101 cgaggccggg ccgccggaga ggatcagccc gcgcgggttg cgctgcttga tctcctcgat
     2161 cggcaccgtg tggtgcagca gctcgctgaa cacgccgcac tcccggacgc gacgcgcgat
     2221 cagctggctg tactggcccc cgaagtccag gaccagcacc tcgtcgtgcg cggccgcggt
     2281 ggcctgcggc tcgacggcct cggcgtcgtc cgccggaacc gtcgagatgg cggcaggcct
     2341 gtccacctac tcgaccagcg ccgcctcggg ctgcgcgggt gcgccgccgt tggtcacgcc
     2401 atcggacgcc ggggcggcct tcgcggccga ctgggagccc atgccgatgg cctggtcgcg
     2461 ctggagctgc ttgccctcgg tctgcagcga cggggccacc atgacctcgg cgcggtggaa
     2521 cgaggggatg tcctcgtagc cgcaggtggc catcgaggtg cgcagcgagc ccatcaggtt
     2581 gaacgtgccg tcgttctcgt gtgccggccc catcaggatc tgctccacgg tgccgttctg
     2641 ggtggtggcc acacgagcgc cgcgcgggag ctcggggtgg aacgtggcca tgccccagtg
     2701 gtagccgtgg ccgggagcct cgtaggcgcg ggccagcggc gagccgatca tcacggcgtc
     2761 ggcgccgcag gcgacggcct tggagatgtc gccgccgttg cgcatgccgc cgtcggcgat
     2821 gaccttgacg tactcgcccg tctccagcat gtgctgcgag cgggcggccg ccacgtcggc
     2881 gatcgccgtg gcctgcggga cgccgatgc
//
LOCUS       DASUPW010000491         3103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_2054374, whole genome shotgun sequence.
ACCESSION   DASUPW010000491 DASUPW010000000
VERSION     DASUPW010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2054374"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..723
                     /locus_tag="VFY44_10960"
     CDS             151..723
                     /locus_tag="VFY44_10960"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEX5911007.1"
                     /translation="MKLVLASRNAHKVRELDELMKPHEVVELPPEVELPPETADTFEG
                     NALGKARAAAAALDSVAIADDSGIEAAALGGRPGVRSARFAGEDATDQENLDKLLADV
                     PADGDTRVAYVCALVAVWPDGRELVTEARCEGRLDHDPRGDGGFGYDPAFVPVDYDDG
                     RTMAELSPAEKDAISHRGRAARQLLELLDG"
     gene            716..1168
                     /locus_tag="VFY44_10965"
     CDS             716..1168
                     /locus_tag="VFY44_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911008.1"
                     /translation="MADLEVSPEALTSALQAEDPAAGIVAAGDLRQGKRPSMVALMLG
                     YGLIVLMLPRKSKQLSRRFVLIATENEVIARRAWSGGGEHEYLIKVGDRLGSWPRDQV
                     WMDGFGADGTQAASATLHLAGQTLPVSRPNLSGDPNTDELFLTLGRAG"
     gene            complement(1165..1305)
                     /locus_tag="VFY44_10970"
     CDS             complement(1165..1305)
                     /locus_tag="VFY44_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911009.1"
                     /translation="MPLSRIAWIVTVGICVIAALLLLVNDYQGYAGVLLAVGAAAAVN
                     LR"
     gene            1324..1665
                     /locus_tag="VFY44_10975"
     CDS             1324..1665
                     /locus_tag="VFY44_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911010.1"
                     /translation="MSVDQRSAPSHLVEMSADVRAAVLADPAGGLVSASAEFDGAPGR
                     ELAGLAADLFTAADRALGDPAQQVEAQVAGGSVFAVRDASHTLACVARRTALPALVLF
                     DLQHVLNEATA"
     gene            1662..1949
                     /locus_tag="VFY44_10980"
     CDS             1662..1949
                     /locus_tag="VFY44_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911011.1"
                     /translation="MRERPKGTVARATYVAEGVATAMRRRQRDKEPRVLLYRRPGDPR
                     VLATGAKGQDAVLEAAEKMVALVDEVLPTPREARRAQRREARAGGAEEDAE"
     gene            1946..>3103
                     /locus_tag="VFY44_10985"
     CDS             1946..>3103
                     /locus_tag="VFY44_10985"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX5911012.1"
                     /translation="MSLVDPELAGRVIARALTHGGDLAELYAEDRRGFSLSLDDGRIE
                     RPQGGNERGASVRVVRGQASYSGHVDGLSEEALLAVAESVAGAVRGDAREPAALSAPV
                     AAEGHRVRVRPEDVPAERQAAILRALDERARAAGPEITQVGVGYVDERRIVEIFNSDG
                     LAASDDRTRVRLSTQAVARRGDRVETGTQTRGGHAGFELLETDPELVAEDAARKALTL
                     LDAVDAPTGRLPVVVGNGFGGVLLHEAVGHGLEADAVQKQASVYAGQLGKQLAERGVT
                     AYDDGAKANAWGSDGIDDEGFPTQRTTIIENGELTSYLYDLVRARKDGVESTGNGRRE
                     SFRHLPVPRMTNTFFAEGTATPEQLIEATERGLYAVSFGGGQVEPATGDFVF"
BASE COUNT          418 a         1129 c         1197 g          359 t
ORIGIN      
        1 gaacgtggtc atgaccggtg acgacggcct ggtcgaggtg caggccacgg gcgagcgcac
       61 cccgctcagc cgcacgttcc tggacgacct gctggtgctg gccgcgggcg ggatcaccga
      121 gctgcgcgag caccagcgca ccgcgacggc gtgaagctcg tcctggcctc acgcaacgcg
      181 cacaaggtgc gcgagctgga cgagctgatg aagccgcacg aggtggtgga gctgccgccc
      241 gaggtcgagc tgccgcccga gaccgccgac accttcgagg gcaacgccct gggcaaggcc
      301 cgggccgccg ccgcggcgct ggacagcgtg gccatcgccg acgactcggg catcgaggcg
      361 gccgcgctcg gcgggcgccc cggggtgcgc tcggcccgct tcgcaggcga ggacgccacc
      421 gaccaggaga acctggacaa gctgctggcc gacgtgccgg ccgacggcga cacccgcgtg
      481 gcctacgtgt gcgcgcttgt ggcggtgtgg cccgacgggc gcgagctggt caccgaggcg
      541 cgctgcgagg gccggctgga tcacgacccc cgcggcgacg gcggcttcgg ctacgacccc
      601 gccttcgtgc ccgtggacta cgacgacggg cgcaccatgg ccgagctgtc gccggccgag
      661 aaggacgcga tcagccaccg gggccgggcc gcgcggcagt tgctggagct gctcgatggc
      721 tgacctcgag gtctcaccgg aggcgctgac gtccgcgctg caggccgagg accccgcagc
      781 gggaatcgtg gcggccggtg acctgcgcca gggcaagcgc ccctcgatgg tggccctgat
      841 gctgggctac gggctgatcg tgctgatgct cccgcgcaag tcgaagcagc tgtcgcgccg
      901 gttcgtgctg atcgcgaccg agaacgaggt catcgcccgg cgcgcctgga gcgggggagg
      961 cgagcacgag tacctgatca aggtgggcga ccggctgggt tcgtggccgc gcgaccaggt
     1021 gtggatggac ggcttcggcg ccgacggcac ccaggcggcg agcgcgacgc tccacctggc
     1081 cggccagacg ctgccggtct cccggccgaa cctgtcggga gacccgaaca ccgacgagct
     1141 gttcctgacg ctcggccgcg ccggctagcg caggttcacg gcagccgccg cgccgacggc
     1201 gagcagcact ccggcgtagc cctggtagtc gttgaccagc aggagcagcg cggcgatcac
     1261 gcagatcccg acggtcacga tccacgcgat ccgagacagt ggcacgcggc tattgtggct
     1321 tgcgtgtcag tcgaccagcg gtccgccccg tcgcacctgg tggagatgtc ggctgacgtg
     1381 agggccgcgg tgctggccga tcccgccggt ggcctggtca gcgcctcggc cgaattcgac
     1441 ggcgcgccgg gccgcgagct ggccgggctg gccgccgacc tgttcaccgc cgccgaccgc
     1501 gccctgggcg acccggcgca gcaggtcgag gcccaggtgg ccggggggtc ggtgttcgcc
     1561 gtgcgcgacg cctcgcacac cctggcctgc gtcgcccgcc gcaccgccct gcccgcgctg
     1621 gtgctcttcg acctccagca cgtcctgaac gaggcgacgg cgtgagggag cggcccaagg
     1681 gcaccgtcgc gcgcgccacg tacgtggccg agggggtggc gaccgccatg cgccggcgcc
     1741 agcgcgacaa ggagccgcgc gtgctgctgt atcgccgccc cggggacccg cgcgtgctgg
     1801 ccaccggcgc caagggccag gacgccgtgc tcgaggcggc cgagaagatg gtggcgctgg
     1861 tcgacgaggt cctgcccacg ccgcgcgagg cccgtcgcgc acagcgccgc gaggcgcgcg
     1921 ccggcggggc ggaggaggac gcggagtgag cctggtcgac cccgagctgg ccgggcgcgt
     1981 gatcgcccgc gcgctgaccc acggcggcga cctggccgag ctgtacgccg aggaccggcg
     2041 cggcttctcg ctgtccctgg acgacggccg gatcgagcgc ccccagggcg gcaacgagcg
     2101 cggcgcgtcg gtgcgcgtgg tgcgcggcca ggccagctac tccggccacg tggacggcct
     2161 ctccgaggag gcgctgctgg cggtggccga gtcggtggcc ggtgccgtcc gcggcgacgc
     2221 ccgcgagccg gccgcgctgt ccgcgccggt ggccgcggag ggccaccggg tccgggtccg
     2281 ccccgaggat gtcccggccg agcgccaggc cgccatcctg cgcgcgctgg acgaacgcgc
     2341 ccgcgccgcc ggcccggaga tcacccaggt gggggtgggc tacgtggacg agcgccggat
     2401 cgtcgagatc ttcaactccg acgggctggc cgcgtccgac gaccgcaccc gcgtgcgcct
     2461 gtcgacgcag gcggtggcgc gccggggcga tcgcgtcgag accggcaccc agacccgcgg
     2521 cggccacgcc ggcttcgagc tgctggagac cgaccccgag ctggtggccg aggacgccgc
     2581 gcgcaaggcg ctgacgctgc tggacgcggt ggacgcgccc accggccggc tgccggtggt
     2641 ggtgggcaac gggttcggcg gcgtgctgct gcacgaggcc gtcggccacg gcctggaggc
     2701 cgacgcggtg cagaagcagg ccagcgtcta cgccggccag ctgggcaagc agctggccga
     2761 gcgcggcgtg accgcctacg acgacggcgc caaggccaac gcctggggca gcgacggcat
     2821 cgacgacgag ggcttcccga cccagcgcac gacgatcatc gagaacggcg agctcacctc
     2881 gtacctctac gacctcgtgc gcgcgcgcaa ggacggcgtg gagtccaccg gcaacggccg
     2941 gcgggagtcg ttccgccacc tgccggtccc gcgcatgacc aacacgttct tcgccgaggg
     3001 gaccgccacg cccgagcagc tcatcgaggc gaccgagcgc ggcctctacg cggtctcgtt
     3061 cggcggcggc caggtcgagc cggccaccgg cgacttcgtc ttc
//
LOCUS       DASUPW010000492         3590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_667092, whole genome shotgun sequence.
ACCESSION   DASUPW010000492 DASUPW010000000
VERSION     DASUPW010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667092"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VFY44_10990"
     CDS             <1..431
                     /locus_tag="VFY44_10990"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX5911013.1"
                     /translation="CTRGGHHAYLGGLIEHTVAVATLVQETSVLHPRLDSDLLMAAAI
                     LHDVGKTREFTFGAEIALSDEGALLGHLALGQQMIVARAGRLDGFPRERLLALVHCVL
                     AHHGPDGLPGRRFGSPEALALARLNAVDAQIKGALEHGIQ"
     gene            428..868
                     /locus_tag="VFY44_10995"
     CDS             428..868
                     /locus_tag="VFY44_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5911014.1"
                     /translation="MTARQAREDDFERVTELLEILGREVVTEETAAECRRVFESQVVG
                     QESAPLVVEDDSGRVIACCSLHYRPRLNRPRPDAWIPDLIVDPDARRQGAGRALLEEA
                     ERLARNRGCWQLTLESGYQREEAHQMYEAFGMANEGYYFGKPLG"
     gene            complement(865..1902)
                     /gene="mgrA"
                     /locus_tag="VFY44_11000"
     CDS             complement(865..1902)
                     /gene="mgrA"
                     /locus_tag="VFY44_11000"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007388.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HEX5911015.1"
                     /translation="MDILEPYVPAAGRYRRMPYRRVGRSGLDLPAISLGLWHNFGDDK
                     PLQTQRDVLRRAFDLGITHFDLANNYGPPYGSAETNFGRLFREDFAAHRDELVISTKA
                     GWDMFPGPYGDLGSRKYLLGSLDRSLERMGLDWVDIFYHHHFDPDTPLEETMGALDTA
                     VRQGKARYVGISSYSPARTREAVEILNSLGTPLLIHQPSYSMLNRWIEDGLLEAVEEE
                     GVGLIAFSPLAQGMLTDRYLKGIPADSRAGLGKDLSKSFLSEENLKRVRALNRIAAKR
                     DQTLAQMAIAWTLRDPRVTSSLIGASSVEQLEDSLAAVKNLKFTDKELKAIDKHAVEG
                     GINLWAGPSHA"
     gene            1945..2601
                     /locus_tag="VFY44_11005"
     CDS             1945..2601
                     /locus_tag="VFY44_11005"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX5911016.1"
                     /translation="MPPLARTQPLPDVVYAALSAQIMRGDLAPGDPLPSERLLAEDYG
                     VNRHAIREAMKRLQQAGLVQVSHGGATRVLDWRATGGLDLLTQVDGPELIRSAVEMRL
                     CIGADAARRCADRAPAPLVERLRALVAQQPAGDQEAADAYEELWGLIVAGADNLAYQL
                     ALNSLLTASTSFDLRERSLPEARDREAQAELVEAIERGDGDAAESLARDLLERTPWST
                     "
     gene            2589..3422
                     /locus_tag="VFY44_11010"
     CDS             2589..3422
                     /locus_tag="VFY44_11010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX5911017.1"
                     /translation="MVDVIVYAIPAFVLLLVVEAVSFRVAAREDLKGYEAKDTRTSLS
                     MGMGNVVINFGWKAVVVVIYAGLYELTPLRVPTEAVWAWVALFFLDDFAYYWFHRISH
                     EVRVFWASHVVHHSSQYFNLSTALRQTWVPMTYFPFWMPIALLGYPPWMILTMQAVSL
                     MYQFWIHTERIGKLPRWFEFIFNTPSHHRVHHGANDLYLDRNYAGILIIWDRMFGTFQ
                     GEEERVRYGLTKNIRWFNPPKVAFHEYAAVWRDVRGADNWRDRLGYMFGGPGWAPPQR
                     R"
BASE COUNT          514 a         1268 c         1264 g          544 t
ORIGIN      
        1 cctgcacgcg cggcgggcac cacgcctacc tgggcggcct gatcgagcac accgtggccg
       61 tggccacgct ggtgcaggag acgtctgtgc tgcacccgcg gctggactcg gacctgctga
      121 tggccgcggc catcctgcac gacgtgggca agacgcgtga gttcacgttc ggcgcggaga
      181 tcgccctgag cgacgagggc gcgctgctcg gccacctggc gctgggccag cagatgatcg
      241 tggcgcgcgc cgggcgcctg gacgggttcc cgcgcgagcg cctgctggcg ctggtgcact
      301 gcgtcctggc ccaccacggc cccgacggcc tgcccggccg gcgcttcggc tcccccgagg
      361 ccctggcgct ggcccgcctg aacgccgtgg acgcccagat caagggcgcg ctcgagcacg
      421 ggatccaatg acggcccggc aagcgcgcga ggacgacttc gagcgcgtca ccgagctgct
      481 cgagatcctg ggccgcgagg tggtcaccga ggagaccgcg gccgagtgcc ggcgggtgtt
      541 cgagtcccag gtcgtcgggc aggagtcggc cccgctggtg gtcgaggacg actcgggccg
      601 cgtgatcgcc tgctgctcgc tgcactaccg cccgcgcctg aaccgccccc gccccgacgc
      661 ctggatcccc gacctgatcg tggaccccga cgcccgccgc cagggcgccg ggcgcgccct
      721 gctcgaggag gccgagcggc tggcccgcaa ccgcggctgc tggcagctga cgctggagtc
      781 cggctaccag cgcgaggagg cccaccagat gtacgaggcc ttcggcatgg ccaacgaggg
      841 ctactacttc ggcaagccgc tgggctaggc gtggctcggg cccgcccaga ggttgatgcc
      901 gccctccacc gcgtgcttgt cgatggcctt cagttccttg tcggtgaact tcaggttctt
      961 cacggcggcc aggctgtcct cgagctgctc gacgctcgag gcgccgatca gcgacgaggt
     1021 cacgcgcggg tcgcgaagcg tccaggcgat ggccatctgc gccagcgtct ggtcccgctt
     1081 tgcggcgatc cggttgagcg cccgcacgcg cttgaggttc tcctcgctca ggaagctctt
     1141 ggacaggtcc ttcccgaggc ccgcgcgtga gtccgccggg atgcccttga ggtagcggtc
     1201 ggtgagcatg ccctgcgcca gcggcgagaa cgcgatcagg cccacgccct cttcctcgac
     1261 cgcttcaagg aggccgtcct cgatccagcg gttgagcatc gagtacgacg gctggtggat
     1321 caggagcggg gtgcccagcg agttcaggat ctccaccgcc tcgcgtgtgc gggcgggcga
     1381 gtaggaggag atgcccacgt accgcgcctt gccctggcgc acggcggtgt ccagggcgcc
     1441 catcgtctcc tcgagcggcg tgtccgggtc gaagtggtgg tggtagaaga tgtcgaccca
     1501 gtcgaggccc atcctctcga gcgacctgtc cagcgagccc agaaggtact tgcgcgagcc
     1561 caggtcgccg tagggcccgg ggaacatgtc ccagccggcc ttcgtcgaga tgaccaactc
     1621 gtcgcggtgc gcggcgaagt cctcgcggaa gagccggccg aagttggtct cggccgatcc
     1681 gtagggcggg ccgtagttgt tggccaggtc gaagtgggtg attccgaggt cgaaggcccg
     1741 gcggagcacg tcgcgctggg tctgcagcgg cttgtcgtcg ccgaagttgt gccacaggcc
     1801 gagcgagatg gccgggaggt cgagtccgct gcggccgacg cggcggtagg gcatgcgccg
     1861 gtagcggccg gcggcgggaa cgtagggctc gaggatgtcc atgcccggca tcatcgcgca
     1921 cccactggta caatcagttg accagtgccg ccgctcgccc gcacgcagcc gctccccgac
     1981 gtggtctacg ccgcgctcag cgcgcagatc atgcgcggcg acctggcccc cggcgacccg
     2041 ctgccctcag agcggctgct ggccgaggac tacggcgtca accgccacgc catccgcgag
     2101 gccatgaagc gcctccagca ggccggcctc gtgcaggtct cgcacggtgg cgccacgcgc
     2161 gtgctggact ggcgcgccac cggcggcctg gacctgctga cccaggtcga cggccccgag
     2221 ctcatccgct cggccgtcga gatgcggctg tgcatcggcg ccgacgccgc ccgccgctgc
     2281 gccgaccgcg ctccggcgcc cctggtggag cggttgcgcg cgctggtggc ccagcagccc
     2341 gccggcgacc aggaggccgc cgacgcctac gaggagctct ggggcctgat cgtggccggc
     2401 gccgacaacc tggcctacca gctcgcgctg aacagcctgc tgaccgcatc gacgtccttc
     2461 gacctgcgcg agcggtcgct gcccgaggcg cgcgaccgcg aggcccaggc cgagctggtg
     2521 gaggcgatcg agcgcggcga cggcgacgcg gccgagtcgc tggcccgcga cctgctcgag
     2581 aggacgccat ggtcgacgtg atcgtctacg ccatcccggc gttcgtgctg ctgctggtgg
     2641 tcgaggcggt ctcgttcagg gtggccgccc gcgaggacct caagggctac gaggccaagg
     2701 acacccgcac cagcctgagc atgggcatgg gcaacgtggt catcaacttc ggctggaagg
     2761 ccgtggtcgt ggtcatctac gccgggctct acgagctgac gccgctgcgc gtacccaccg
     2821 aggcggtctg ggcctgggtc gcgctcttct tcctggacga cttcgcctac tactggttcc
     2881 accggatcag ccacgaggtg cgcgtgttct gggccagcca cgtggtccac cacagctcgc
     2941 agtacttcaa cctgtcgacc gcgctgcgcc agacctgggt gcccatgacc tacttcccgt
     3001 tctggatgcc gatcgcgctg ctgggctacc cgccgtggat gatcctgacc atgcaggccg
     3061 tgagcctgat gtaccagttc tggatccaca ccgagcggat cggcaagctg ccgcgctggt
     3121 tcgagttcat cttcaacacc ccgagccacc accgcgtgca ccacggcgcc aacgacctct
     3181 acctggaccg caactacgcc ggcatcctga tcatctggga ccgcatgttc ggcaccttcc
     3241 agggcgagga ggagcgcgtg cgctacgggc tgaccaagaa catccgctgg ttcaacccgc
     3301 cgaaggtcgc cttccacgag tacgcggccg tgtggcgcga cgtgcgcggc gccgacaact
     3361 ggcgcgaccg gctgggctac atgttcggcg gccccggctg ggcgccaccg cagcgcaggt
     3421 aatctcacgg agtgttctac gaccacgtga ccggcccgtt gtgggccgag ctggcctcgc
     3481 aggtgcccgg cctggacgtg atcgacgtgc acacgcatat cgggcagaac gatcccgacg
     3541 gcttcacgtg cacgacgcgg gagctgcgtg acggcctgga cacgatcggc
//
LOCUS       DASUPW010000493         2796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_667263, whole genome shotgun sequence.
ACCESSION   DASUPW010000493 DASUPW010000000
VERSION     DASUPW010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667263"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VFY44_11015"
     CDS             <1..361
                     /locus_tag="VFY44_11015"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5911018.1"
                     /translation="DLDPSTPVLGTEVFGPLVTVEAVASVEAACDVVDALPFALTGGL
                     FSRNPDVVRRVSHRSPVGNLYVNRGVTGAMVGRQPFGGNRLSGSGSKAGGPDYLAQFA
                     EPRVVTQNVMRHGLVVD"
     gene            372..1475
                     /locus_tag="VFY44_11020"
     CDS             372..1475
                     /locus_tag="VFY44_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX5911019.1"
                     /translation="MKMRAAVLEEFGQPLVVQEVELAEPQEGEVLVRLEACGVCHTDL
                     YTASGADPSGYAPTVLGHEGAGIVEKVGRGVSLVREGDRVVTLFSPQCGECEHCISPR
                     TNLCLAIREEQGKGHLPDGTTRLSRGGEDIRHFMGTSTFAEYTVMPEIALAVVSPDAP
                     ADRVCVFACGLSTGLGAAINTAGVEPGSTVVVYGAGLVGLGAVAGARLQGADRIVCVD
                     LSEERLEMARGQGATDTFVGGPDSVERVLEMTGGMGADYTFEATGNVNVMRQAVESAR
                     MGWGLCTVTGVAGKGETLDVIPRFLITGRRIAGSSFGGVKGRDQVPVFVERYLDGEID
                     VDSLISHKLSLDQVNEGFDLMHAQDGIRSVIDF"
     gene            1475..2284
                     /locus_tag="VFY44_11025"
     CDS             1475..2284
                     /locus_tag="VFY44_11025"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5911020.1"
                     /translation="MPATVLALHGITSNGRAWDAVARELSGDVRLLTPDLRGRGDAGE
                     ASGPYGLARHVEDAVGVLDAAGLDRAVVAGHSMGAYVTALLAATHPDRVRSVVLVDGG
                     PPLPVPEGADPDALLEATLGPSIERLSREFETPEAYHEFWRAHPAFAGYDVADGDLTA
                     YADHDLRGEPPHLRSSVAEEAVRTDGRELIADHDVRTALDAVRAPGVLLRAQRGLLDQ
                     PEAFIPTDMASAFDHPTVELRDVPDSNHFTILMGAAGARAVADAIRAAASA"
     gene            complement(2281..2736)
                     /locus_tag="VFY44_11030"
     CDS             complement(2281..2736)
                     /locus_tag="VFY44_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5911021.1"
                     /translation="MNPDIPKLVQILLVEDNPEEVLLTRETLKDAKVANEVHVAEDGE
                     RALRFARREGEYASAPRPDLILLDLNLPRKDGREVLSELKADEDLRRIPVIVLTTSAS
                     DQDMLQAYDNAVNAYIRKPVSLDRFIEVVNSIDDFWLGVVALPSARRAA"
BASE COUNT          356 a          988 c         1054 g          398 t
ORIGIN      
        1 ggacctggac ccgtcgacgc cggtcctggg caccgaggtg ttcggcccgc tggtcaccgt
       61 agaggcggtc gcctccgtcg aggccgcctg cgacgtggtc gacgcgctgc cgttcgccct
      121 gaccgggggc ctgttctcac gcaacccgga cgtcgtgcgc cgcgtctcgc accgctcgcc
      181 ggtgggcaac ctgtatgtga accgcggcgt gaccggcgcc atggtcgggc gccagccctt
      241 cggcggcaac cgcctgtcgg gcagcggctc gaaggccggc gggcccgact acctggcgca
      301 gttcgccgag ccgcgcgtgg tcacccagaa cgtgatgcgc cacggcctcg tggtggatta
      361 gtctcgccgc catgaagatg cgcgccgcag tcctggagga gttcggtcag ccgctggtgg
      421 tccaggaggt ggagctggcc gagccgcagg agggcgaggt gctggtgcgc ctggaggcct
      481 gtggggtctg ccacaccgac ctgtacaccg cctccggcgc cgacccgtcg ggctacgcgc
      541 ccacggtgct gggccacgag ggggccggca tcgtcgagaa ggtgggccgg ggcgtgtcgc
      601 tggtgcgcga gggcgaccgt gtggtcacgc tgttcagccc ccagtgcggc gagtgcgagc
      661 actgcatcag cccgcgcacg aacctgtgcc tggccatacg cgaggagcag ggcaagggcc
      721 acctgcccga cggcaccacg cgcctgagcc gcggcggcga ggacatccgc cacttcatgg
      781 gcacctccac gttcgccgag tacaccgtga tgccggagat cgcgctggcg gtggtcagcc
      841 ccgacgcccc ggccgaccgc gtgtgcgtgt tcgcgtgcgg gttgtccacc ggcctcggcg
      901 ccgccatcaa caccgcgggg gtggagcccg gctcgaccgt ggtggtctac ggcgccggcc
      961 tggtgggcct gggcgcggtg gccggcgcgc ggctgcaggg cgccgaccgg atcgtgtgcg
     1021 tcgacctgtc cgaggagcgg ctggagatgg cccgcggaca gggcgcgacc gacacgttcg
     1081 tgggcggccc cgacagcgtg gagcgggtgc tggagatgac cggcggcatg ggcgccgact
     1141 acacgttcga ggccaccggc aacgtgaacg tgatgcgcca ggcggtggag tccgcgcgca
     1201 tgggatgggg cctgtgcacg gtcaccgggg tcgccggcaa gggcgagacg ctggacgtga
     1261 tcccgcgctt cctgatcacc ggccgccgga tcgccgggtc gtcgttcggg ggcgtgaagg
     1321 gccgcgacca ggtgccggtg ttcgtggagc gctacctgga cggcgagatc gacgtggact
     1381 cgctgatctc gcacaagctg tcgctggacc aggtgaacga aggcttcgac ctgatgcacg
     1441 cccaggacgg catccgcagc gtcatcgact tctgatgccc gccaccgtcc tggccctgca
     1501 cgggatcacc tcgaacggcc gcgcgtggga cgcggtggcc cgcgagctca gcggtgacgt
     1561 ccgcctgctg acccccgacc tgcgcggccg cggcgacgcg ggcgaggcat cggggcccta
     1621 cggcctggcg cgccacgtcg aggacgccgt cggcgtgctg gacgccgccg gcctggaccg
     1681 cgcggtcgtg gccgggcatt cgatgggggc gtacgtgacc gcgctgctgg ccgccaccca
     1741 ccccgaccgg gtgcggtcgg tggtgctggt ggacggcggt ccgcccctgc ccgtgcccga
     1801 gggggccgac ccggacgcgc tgctggaggc gaccctgggc ccgtcgatcg agcggctctc
     1861 gcgtgagttc gagacccccg aggcctacca cgagttctgg cgcgcgcacc cggccttcgc
     1921 cggctacgac gtggccgacg gcgacctgac cgcctacgcc gaccacgacc tgcgtggcga
     1981 gccgccccac ctgcgctcca gcgtggccga ggaggcggtg cgcaccgacg gccgcgagct
     2041 gatcgccgac cacgacgtgc gcacggcgct ggacgcggtg cgcgcgcccg gggtgctgct
     2101 gcgcgcgcag cgcggcctgc tggaccagcc cgaggccttc atacccacgg acatggcgtc
     2161 cgcgttcgac caccccacgg tggagctgcg ggacgtgccc gacagcaacc acttcacgat
     2221 cctgatgggc gccgccggcg cccgcgcggt cgcggacgcg atccgcgccg cggcgtccgc
     2281 ctaggccgcg cggcgcgcgc tgggcagcgc caccacgccc agccagaagt cgtcgatgga
     2341 gttcacgacc tcgatgaagc ggtccaggct gaccggcttg cggatgtagg cgttcacggc
     2401 gttgtcgtag gcctggagca tgtcctggtc ggacgccgag gtggtcagca cgatcaccgg
     2461 gatccgccgg aggtcctcgt cggccttgag ctcgctgagc acctcgcggc cgtccttgcg
     2521 gggcaggttc aggtccagca ggatcaggtc gggccgcggg gccgaggcgt actcgccctc
     2581 ccggcgggcg aagcgcagcg cgcgctcgcc gtcctcggcc acgtgcacct cgttcgccac
     2641 cttggcgtcc ttgagcgtct ccctggtcag gagcacctct tcggggttgt cctcgaccag
     2701 gaggatctgg acgagcttgg ggatgtcggg gttcatgagc gctcctgagg cagtgtgaag
     2761 ttgaataccg tccccccgcc ggcgcccgct tcggcc
//
LOCUS       DASUPW010000494         2380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1083692, whole genome shotgun sequence.
ACCESSION   DASUPW010000494 DASUPW010000000
VERSION     DASUPW010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083692"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="VFY44_11035"
     CDS             <1..1108
                     /locus_tag="VFY44_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5911022.1"
                     /translation="RLLQQTVRDFAVQEVGPAAEELDRTKSFPYELVAKMAELGWMGI
                     PFPEEVGGAGGTSLQYAIAVEELTRADSSVAITMCAHTSLGTQPIYLFGNQDQKDSYL
                     PDLCAGTKLGAFGLTEPEAGSDAGNVRTRAKLEDGEWVIDGAKQFITNAGTDISGHVA
                     ITARTGEKEISNLIVDAGTPGYEAGEPYRKMGWNASDTRPLSFDGARVPEENLLGPRG
                     QGFRQFLKVLDIGRIGVAAMGVGLAQGALDEAVAYAKERQAFGQSISKFQAIQTKLAD
                     MATEIAAARYLTYKAAVEKDLGRDFSQTAAMAKLKTGRLAVRCAEEAVQIHGGYGYIE
                     EYPVCRFYRDAKILTIGEGTDEVQHMVIARGLGA"
     gene            complement(1139..1882)
                     /locus_tag="VFY44_11040"
     CDS             complement(1139..1882)
                     /locus_tag="VFY44_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911023.1"
                     /translation="MNPIIRTTVLALSVLGLAITPVAEAAKPPVKVKRARFAVTVDGV
                     QNTTWSTNHVAAGIGGCDGSYTAGGTEKVRFTSKARTIRAMVIPGLSAPVLSTGTGSN
                     SRSPKLKLHGSITRDGSHTSAPGPDTGCGGADRPQVLTKDCGTKKFAGLVTSFDYKLA
                     SKVRDTLDVSATDGDDPFQRCPSGGVSFPTLAHTYDGRPMALRMPRGELFNRKLGKII
                     LIGRAREQKQDGEHKYTTTTKWVITLVRK"
     gene            2016..>2380
                     /locus_tag="VFY44_11045"
     CDS             2016..>2380
                     /locus_tag="VFY44_11045"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5911024.1"
                     /translation="MDRTLVERVREVEELYSLIGDTAKGEGAVALIEGTAGVGKSSLL
                     AQVRARAQRDGARVLTARGSELESLFTFGVVRQLFEGVLADKKARAGALKGAAAPAEA
                     AFGPPGDDGEPGGSESFTTL"
BASE COUNT          348 a          821 c          873 g          338 t
ORIGIN      
        1 caggctcctc cagcagaccg tgcgcgactt cgccgtgcag gaggtcggcc cggcggccga
       61 ggagctggac cgcaccaagt ccttccccta cgagctggtg gccaagatgg ccgagctcgg
      121 ctggatgggc atcccgttcc ccgaggaggt cggcggtgcg ggcggcacgt cgctgcagta
      181 cgcgatcgcg gtcgaggagc tgacgcgggc ggactcgtcg gtcgcgatca cgatgtgcgc
      241 gcacacctcg ctgggcacgc agccgatcta cctgttcggc aaccaggacc agaaggattc
      301 ctacctgccc gacctgtgcg ccggcaccaa gctgggcgcc ttcgggctga ccgagcccga
      361 ggccggctcg gacgccggca acgtgcgcac gcgcgcgaag ctggaggacg gcgagtgggt
      421 gatcgacggc gccaagcagt tcatcaccaa cgcgggcacc gacatctcgg gccacgtggc
      481 catcaccgct cgcaccggcg agaaggagat ctccaacctg atcgtggacg ccggcacccc
      541 cggctacgag gcgggcgagc cctaccgcaa gatgggctgg aacgcctccg acacccggcc
      601 gctgagcttc gacggcgcgc gcgttcccga ggagaacctg ctgggccccc gcggccaggg
      661 cttccgccag ttcctgaagg tgctggacat cgggcgcatc ggcgtggccg ccatgggcgt
      721 cggcctggcc cagggcgccc tggacgaggc cgtggcctac gccaaggagc gccaggcctt
      781 cggccagtcg atctccaagt tccaggccat ccagaccaag ctggccgaca tggccacaga
      841 gatcgcggcc gcccgctacc tgacctacaa ggcggcggtc gagaaggacc tggggcgcga
      901 cttctcccag accgcggcca tggccaagct caagaccggc cgcctggcgg tgcgctgcgc
      961 cgaggaggcc gtgcagatcc acggcggcta cggctacatc gaggaatacc ccgtctgccg
     1021 cttctaccgc gacgcgaaga tcctgaccat cggcgagggc accgacgagg tgcagcacat
     1081 ggtcatcgcc cgcggcctgg gcgcgtagcc gggccgcggg cgcctcgagc ggcccgggct
     1141 acttgcggac cagcgtgatc acccacttgg tggtggtcgt gtacttgtgc tcgccgtcct
     1201 gcttctgctc gcgggcgcgg ccgatcagga tgatcttgcc gagcttgcgg ttgaacagct
     1261 cgccgcgcgg catccgcagg gccatcggcc ggccgtcgta cgtgtgggcc agggtcggga
     1321 agctcacgcc gccgctcggg cagcgctgga acggatcgtc gccgtcggtg gcgctgacgt
     1381 ccagcgtgtc gcggaccttg gaggccagct tgtagtcgaa gctggtgacc aggccggcga
     1441 acttcttcgt gccgcagtcc ttggtcagca cctgcggcct gtccgcgcca ccgcagcccg
     1501 tgtccgggcc cggcgcggag gtgtgggacc cgtcccgcgt gatgctgccg tgcagcttga
     1561 gcttgggcga gcgggagttc gagcccgttc ccgtgctcag caccggagcg ctcagccccg
     1621 ggatgaccat tgcccggatg gtgcgcgcct tcgaggtgaa gcggaccttc tcggtcccac
     1681 ccgcggtgta ggacccgtcg cagccaccga tgccggcggc cacgtggttg gtcgaccagg
     1741 tggtgttctg gacgccgtcc accgtcacgg cgaagcgggc ccgcttgacc ttgaccggcg
     1801 gcttggccgc ctcggcgacg ggggtgatgg ccagccccag cacgctcagt gccaggacgg
     1861 tggtgcggat gatggggttc acggggtctc ctatgcgttg gtggggtgaa cgtctgccat
     1921 caccctcggc gcggcggcgc acaggggcat cgagggcgcc cctagacaga ccccccaagg
     1981 tttcgggcac ccggcacctg acctacaatc gcgaggtgga caggacgctg gtcgagcgcg
     2041 tgcgcgaggt cgaggagctg tactccctga tcggcgacac ggcgaagggc gaaggggccg
     2101 tggcgctgat cgagggcacg gccggagtgg gcaagagctc gctgctggcg caggtccgcg
     2161 cccgcgccca gcgcgacggc gcccgggtgc tgaccgcccg cggcagcgag ctggagagcc
     2221 tgttcacgtt cggggtggtg cgccagctgt tcgagggcgt gctggccgac aagaaggcgc
     2281 gcgccggcgc gctcaagggc gccgccgcgc cggccgaggc ggccttcggg ccgcccgggg
     2341 acgacgggga gcccggcggc agcgagtcct tcaccaccct
//
LOCUS       DASUPW010000495         3104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1776765, whole genome shotgun sequence.
ACCESSION   DASUPW010000495 DASUPW010000000
VERSION     DASUPW010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776765"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /locus_tag="VFY44_11050"
     CDS             <1..1210
                     /locus_tag="VFY44_11050"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5911025.1"
                     /translation="EGLQPVKEPAVGVAGAPAGRVSKVRIDRGRARVTMRVDADFEGK
                     IFKDATATVRPTSALQTLTVNVSPGTPASGDLEAGDTIPPARTGSFVHVDELTSTLDA
                     DTQAQVQVLIRELAGALDGREPELRQIVRELGRLTDGATPLAGALADRRVLLRRLTDN
                     LDVMLTTLGTRGGQLASAVHLGNRTLEVTERRRPQIEASLRELAPLLSETQQALRAGR
                     GLAEPLSPALDQLIPVTDDARLAAARLRTTLPPLNRFVGTAAGVARDGRRPARLLAGG
                     LKNQAELIRNDQTPALKELIGLVELLERNRHGVIKFARNVSGVTSINRRAGTFGQFDI
                     LNFETSAGGFGFGRSATRSRDGKPSRLALDLGEMLEHSCRDDNAVACQIRFQIPGLPR
                     GSIIESRDGR"
     gene            1210..2586
                     /locus_tag="VFY44_11055"
     CDS             1210..2586
                     /locus_tag="VFY44_11055"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5911026.1"
                     /translation="MDHRVPRVGAWIGLVLALAAMVTFVFLNNRFEGPGDPIKRIGGP
                     TELTATFANTKRLPSKQPVLFKGLEVGRVNRIEWDRRRRDATVTFTLKDDFELRRDAV
                     VRIGERSLIGDPYLDVVTRGSRSLPVLGDGDRVANTKPTVDFDEALDFLDEDGRADVR
                     SVLGTVARGVPASRREPLNDTVGNLSRTIAEARELTSAVRGQEEQIAGLVRSTGVVVD
                     ELGRRETSIRTIVGSGRVTLDALASDTRSLERGVAELPALLASGQRSLALARPLVSEL
                     RGPVRDLRALSPDLARAFDTGRSHSVRSVSVDLDATLRAMPRLRRVAVPILNKDILPV
                     TRNLTPLVKAVRPPARSLVPALEYLAAGDPGTSRAEAIAGLYASLGAANKGTNGTRGH
                     YSRAGFSLSLPDLLDRPADDCPTSGFCNNAYPSSGDALDPQPFRGSYPRIKECTVPPR
                     SRPTASCK"
     gene            complement(2588..>3104)
                     /locus_tag="VFY44_11060"
     CDS             complement(2588..>3104)
                     /locus_tag="VFY44_11060"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX5911027.1"
                     /translation="VAACLYWGTADLFRLLGMDLGPARAEALYQYSKRLGTTLQVRED
                     AWPADLAAFDRYWEEGLKEIRMDDLTRGYLQGIAQLEFLTAPLGRLGAPLRPLLRPYG
                     RFITLGYLPERFRRELGLPWSARHQRLFDLHIRLYAAVTRRLPPALRQFPFNLYLGDV
                     RRRIRRGDPIV"
BASE COUNT          423 a         1147 c         1144 g          390 t
ORIGIN      
        1 cgaggggctc cagcccgtca aggagccggc ggtcggggtg gcgggcgcgc cggcgggccg
       61 ggtctccaag gtccggatcg accgcgggcg ggcgcgggtc acgatgcgcg tggacgccga
      121 cttcgagggc aagatcttca aggacgccac ggccacggtc aggcccacca gcgcgctgca
      181 gacgctgacc gtgaacgtct cgccgggcac gcccgccagt ggcgacctgg aggccggcga
      241 cacgatcccg cccgcgcgga cggggtcgtt cgtgcacgtc gacgagctga cctcgacgct
      301 ggacgccgac acccaggcgc aggtccaggt gctgatccgc gagctggccg gggcgctgga
      361 cgggcgcgag ccggagctgc gccagatcgt gcgcgagctg ggccggctga ccgacggggc
      421 cacgccgctg gccggtgcgc tggccgacag gcgggtgctg ctccggcgcc tcacggacaa
      481 cctggacgtg atgctcacca cgctggggac gcggggcggg cagctggcct ccgcggtcca
      541 cctgggcaac cggacgctcg aggtgaccga gcgccgccgg ccgcagatcg aggcatcgct
      601 gcgcgagctg gcgccgctgc tgtccgagac gcagcaggca ctgcgcgcgg gccgtgggct
      661 ggccgagccg ctgtcaccgg ccctcgacca gctgatcccg gtgaccgacg acgcccgcct
      721 ggccgccgcg aggctgcgga ccacgctgcc ccctctgaac cggttcgtcg gcacggcggc
      781 gggggtcgcc cgcgacggcc gccgtcccgc ccggctgctg gccggcgggc tcaagaacca
      841 ggccgagctg atccgcaacg accagacccc cgccctgaag gagctgatcg ggctggtcga
      901 gctgctcgag cgcaaccgcc acggcgtgat caagttcgcc cgcaacgtca gcggggtcac
      961 gtcgatcaac cgccgtgccg ggaccttcgg gcagttcgac atcctgaact tcgagacctc
     1021 ggccggaggg ttcggcttcg gccggtccgc cacccgcagc cgggacggca agccgtcgcg
     1081 gctggcgctc gacctcggcg agatgctcga gcacagctgc cgtgacgaca acgccgtggc
     1141 ctgccagatc cggttccaga tcccgggcct gccgcggggc tcgatcatcg agtccaggga
     1201 cggtcgctga tggaccatcg cgtcccccgc gtcggtgcct ggatcggcct cgtgctcgcg
     1261 ctggccgcga tggtcacgtt cgtcttcctg aacaaccggt tcgagggccc gggcgacccg
     1321 atcaagagga tcggcggccc gaccgagctg accgcgacct tcgcgaacac caagcgcctg
     1381 cccagcaagc agccggtcct gttcaagggc ctcgaggtcg ggcgcgtgaa ccggatcgag
     1441 tgggaccgcc gccggcgcga cgccacggtc accttcaccc tgaaggacga cttcgagctg
     1501 cgccgcgacg cggtggtgcg gatcggcgag cgcagcctga tcggggaccc gtacctggac
     1561 gtggtcaccc ggggctcgcg gagcctgccc gtgctgggag acggcgaccg ggtcgccaac
     1621 accaagccca cggtggactt cgacgaggcg ctggacttcc tggacgagga cgggcgcgcc
     1681 gacgtccggt ccgtgctggg gaccgtggcc cgtggagtgc ccgcctcccg ccgcgagccg
     1741 ctgaacgaca ccgtcgggaa cctgtcgcgc accatcgctg aggcgcgcga gctgacgtcc
     1801 gccgtccgcg gtcaggagga gcagatcgcc gggctggtcc ggagcaccgg cgtggtggtc
     1861 gacgagctcg gccggcgcga gacgtcgatc cgcacgatcg tcgggtccgg gcgcgtcacg
     1921 ctcgacgcgc tggcctccga cacccggtcg ctcgagcgcg gggtggcgga gctgcccgcc
     1981 ctgctggcct ccgggcagcg gtcgctggcg ctcgcgcggc cgctcgtcag cgagctgcgg
     2041 gggccggtgc gcgacctgcg cgccctgagc ccggacctgg cgcgtgcgtt cgacaccggc
     2101 cggtcgcact ccgtgcgcag cgtctcggtc gacctggacg cgacgctgcg cgcgatgccg
     2161 cggctacgcc gcgtcgcggt cccgatcctg aacaaggaca tcctgcccgt gacccggaac
     2221 ctcacgcccc tggtcaaggc ggtgcgccca cccgcccgca gcctggtccc ggccctggag
     2281 tacctggcgg ccggcgatcc gggcacctcg cgggccgagg ctatcgccgg gctgtacgcc
     2341 tcgctcggtg ccgcgaacaa gggcaccaac ggcacccgcg gccactactc gcgggccggc
     2401 ttctcgctgt cgctgcccga cctgctggac cgcccggccg acgactgccc gacgtcgggg
     2461 ttctgcaaca acgcctaccc ctcgtcgggc gacgcgctcg acccccagcc cttccgcggc
     2521 tcgtacccgc ggatcaagga gtgcacggtg cccccgcgca gccgcccgac ggcgtcgtgc
     2581 aagtgagcta gacgatgggg tcgccgcggc ggatccgccg gcggacgtcg cccaggtaca
     2641 ggttgaacgg gaactggcgc agcgcgggcg gcaggcgccg cgtgaccgcc gcgtagaggc
     2701 ggatgtgcag gtcgaagagg cgctggtgac gcgcgctcca gggcagtccc agctcgcggc
     2761 ggaagcgctc cgggaggtag cccagcgtga tgaagcgccc gtacgggcgc agcagcgggc
     2821 gcagcggtgc gccgaggcgt cccaggggcg cggtcaggaa ctcgagctgt gcgatcccct
     2881 gcaggtagcc gcgcgtcagg tcgtccatcc tgatctcctt gaggccctcc tcccagtagc
     2941 ggtcgaaggc cgccaggtcg gccggccacg cgtcctcgcg cacctggagc gtcgtgccca
     3001 ggcgcttgga gtactggtac agggcctcgg cccgggccgg tcccaggtcc atccccagca
     3061 gccggaacag gtcggccgtc ccccagtaca ggcacgccgc cacc
//
LOCUS       DASUPW010000496         6816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_807374, whole genome shotgun sequence.
ACCESSION   DASUPW010000496 DASUPW010000000
VERSION     DASUPW010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6816
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807374"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VFY44_11065"
     CDS             complement(<1..464)
                     /locus_tag="VFY44_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911028.1"
                     /translation="MRTRLLAAALTAVALAVPMLVLQRWIAGTRSAAIVMVAAFLVVF
                     AVGVAVVCRRRPELRLPVAGTFAAVVAGTLAIGWWTGFRDHAVDEDVATASKRAAGGE
                     RDRALRGEPRRARRSAPVVLATGRFTGKDGHDGRGTATVVRRDGDRVLTFTR"
     gene            complement(485..1129)
                     /locus_tag="VFY44_11070"
     CDS             complement(485..1129)
                     /locus_tag="VFY44_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5911029.1"
                     /translation="MSSAQGERPIKRRMRAPARRAEVLNAAVECFARSGYRATSMGEI
                     AARADVARPVLYDHFPSKKALFLEVLDEQSGRFLADVASRISGEGDARERMRQTIAAV
                     FAFAHERQDPWRLLFGNTAHGDPEIDAAAAAAHRDRVAAVAALLAEDARGAGIDPAGV
                     RAEAMVEMLIAALRGGAEWGLSRPGLSRDDLVDAALDLLWTGLGNLRRDVPPPR"
     gene            1206..3005
                     /locus_tag="VFY44_11075"
     CDS             1206..3005
                     /locus_tag="VFY44_11075"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5911030.1"
                     /translation="MSEAGAGQGPGRGLTRRGFVRDAGMAAAAVGLAGPLAAPAIGAP
                     RGRRVAVLGGGMAGLTVAHELVERGFKVDVYEPVALGGKARSIDVPGSGKGRRRPLPG
                     EHGFRFFPGFYHHIPDSMRRTPDGKNAEGVYDNLVDTSEGRSVRADGRADAQLFGMIP
                     DPVAATTPQGLQALLIEEIVKMKMVPPHEAGYLASRLAVFLTSSEERRYGQWEHVAWW
                     DYVGASSRSEEYQKVAARGLTRSLVAAKETVASTRTIGNMAEAFIMNIMQRGNDGALD
                     RVLDAPTNEAWIQPWVKMLRRRGVRFHLGQRAVALDVRGGRVRSAVVRDRRGRRRRVS
                     ADWFVSAMPAERMVPLLSRQLLRADPALEGIRSLQVDWMAGVQYYLPKKIDLTHGHVT
                     YVDSPWALTSLTQAQFWAERDFPKTYGDGKTVDCLSVDISDWDSPGLLVKKPAKRCTP
                     AEIKREVWHQITEHLKDTDPAILQLGSPRAFLDPGIVWDRRTRRNRNATPLLVNTVGS
                     WEKRPKAKTAVSNLFLSGDYVQTDIDLATMEGANESGRAAVAGLLDAAGSSAAKPKMF
                     KLYDPPEFEAAKQADKALYAQGLPHVLDGPGAR"
     gene            3002..4432
                     /locus_tag="VFY44_11080"
     CDS             3002..4432
                     /locus_tag="VFY44_11080"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX5911031.1"
                     /translation="MTEHVDAIVVGARCAGAATATALARAGRRVVALERARFPADTLS
                     THLLFLGGVLELQRLGALERVLELGPPKLTEASMGWGPFEAEAGYTPVQGIDYALCVR
                     RPGLDAALVATARDAGAQVREQSTVREVLVEDGRAAGVRYRDEDGSEHELCAPLVVGA
                     DGRRSAVARAMGADAPELESPNGRACFFAYWRDGGGGSRSLASQWRQGRELVTAFPCD
                     DGLVLVLLMPPVERAEDFAADLAGEYDRTVASVPQLAERLRGATRQSKVRHTTKTASY
                     FRRSSGPGWALPGDAGHFKDPVTAQGIRDAMRFGRLLGEQAAPALDDPRRLDRALRRW
                     EQRRDRECLPMYAWTNRLARGEEMGALEIELYRRGAGDPELTRRFLDVMARTRTPAQA
                     FGPATKASLALAAARRPGGGRAVAREVGRELRDVLAERVERGRLWSATRWRGRPVRPR
                     RTRTRPLPGGTAPARRAGAGPARPAR"
     gene            complement(4317..4817)
                     /locus_tag="VFY44_11085"
     CDS             complement(4317..4817)
                     /locus_tag="VFY44_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911032.1"
                     /translation="MSDHLPTYLNDHLAGSTAGLELARRAAGSNEGTELGTLLSRFAS
                     EFEGERSSLKSVIEAVGSHPNPAKVAAAWVGEKAGRLKPNDQLTGYSALSRMVELEGL
                     SIGIEGKRLLWRALAERTDPRLTSFDFTALAEQAQRQRDELEPFRLAAAASAFDADAQ
                     GAPATA"
     gene            4914..5567
                     /locus_tag="VFY44_11090"
     CDS             4914..5567
                     /locus_tag="VFY44_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911033.1"
                     /translation="MRLGVRLNVVLILALSCALTAGVGVAGADGSGAAPGAEATAKKK
                     KCGKGKTRVKRKGRSSVCATKCKAGFKHNVSKRGKVTCGRKPGSKQRTPQGGSGPKAG
                     NYTGTTAQNRTMTFTVAGGQVTNFQAGVNTYCSTPGNSRFQIDAIANIPPMAIGPDGS
                     FSYKGTSDQGNPEIAGKITGGSATGTVGMSRGDSNYSGGQLTFGSCSASGVTWNAAG"
     gene            complement(5581..6246)
                     /locus_tag="VFY44_11095"
     CDS             complement(5581..6246)
                     /locus_tag="VFY44_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911034.1"
                     /translation="MGFEWLFGPVEHADGWLTALFEGQPLLVALLIALLLGLRHASDP
                     DHVLAVTSLMASEDADTRAAARLGAWWGAGHAATLLMVGAPLIVLESGLPPWLESGAE
                     RAVGAVIVVLALRMLFRWARGHRTTVRSPRQALGIGVLHGLAGTGAVVLLLIAALPTR
                     GQAVAALAVFAPMSAVSMALCSGGVAWTLTRERVEPLYRTVVIPALGTFGVLFGLWYA
                     GLA"
     gene            6268..>6816
                     /locus_tag="VFY44_11100"
     CDS             6268..>6816
                     /locus_tag="VFY44_11100"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291634.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX5911035.1"
                     /translation="MSELAPDYFTKHLADVDPEIAQVVSDEERRQEATLEMIASENFV
                     PQAILEAQGSVATNKYAEGYPGRRYYGGCEHVDVAEQLAIDRAKALFGAEHANVQPHA
                     GAQANTAVYHALLQPGDTIMGLSLPHGGHLSHGMKINFSGRLYDIVAYGVREEDSRLD
                     MDELAEIVADARPKLILAGWSAY"
BASE COUNT          979 a         2457 c         2567 g          813 t
ORIGIN      
        1 aagcgggtga acgtgagcac ccggtcaccg tcgcggcgta cgacggtcgc cgtgccgcgc
       61 ccgtcgtgcc cgtccttgcc ggtgaagcgg cccgtggcca gcacgaccgg cgcggaccgc
      121 ctggcccgcc gcggttcgcc gcgcagggcg cggtcgcgct caccgcccgc ggcgcgcttc
      181 gaggcggtgg cgacgtcctc gtccacggcg tggtcgcgga acccggtcca ccagccgatg
      241 gccagcgtgc ccgccacgac cgcggcgaac gtgcccgcga ccgggagccg cagctccggc
      301 cggcgacggc agacgacggc gaccccgacc gcgaacacga ccaggaaggc cgcgaccatg
      361 acgatggccg ccgacctcgt ccccgcgatc cagcgctgga gcacgagcat cggcaccgcg
      421 agggcgaccg ccgtgagcgc cgccgccagg agccgtgtcc gcatggggac acgctagccg
      481 aggctcagcg gggcgggggc acgtcccggc gcaggtttcc caggcccgtc cacagcaggt
      541 ccagcgccgc gtccaccagg tcgtcccgcg acagccccgg gcgcgacagg ccccactcgg
      601 cgccgccgcg cagggcggcg atcagcatct cgaccatcgc ctcggcgcgg acgcccgcgg
      661 ggtcgatgcc ggcgccacgg gcgtcctcgg ccagcagcgc cgccaccgcc gcgacgcgat
      721 cgcggtgggc cgcggcggcc gccgcgtcga tctcggggtc gccgtgcgcg gtgttgccga
      781 acagcagccg ccacgggtcc tggcgctcgt gggcgaacgc gaacaccgcc gcgatcgtct
      841 ggcgcatccg ctcgcgggcg tcgccctcgc cggagatgcg gctggccacg tcggccagga
      901 accggccgct ctgctcgtcc agcacctcca ggaagagcgc cttcttggag gggaagtggt
      961 cgtacaggac gggcctggcc acatccgccc gggctgcgat ctcgcccatc gaggtggctc
     1021 ggtagcccga ccgggcgaag cactcgaccg ccgcgttcag cacctccgcg cgccgggccg
     1081 gcgcgcgcat gcggcgcttg atcggacgct cgccctgggc gctggacacg gcggcagcgt
     1141 attcgttgac aaccgacaca cctgtaggtt atcttcctgg cgcggaaaca cacgggaggg
     1201 gaccgatgag tgaggcaggg gcagggcaag gacccgggcg ggggctcacg cggcgcggct
     1261 tcgtgcgcga cgcgggcatg gcggcggccg cggtcggact ggccggaccg ctggcggcac
     1321 cggcgatcgg cgcaccacgg ggcaggcggg tggccgtgct cgggggcggc atggcgggcc
     1381 tgacggtggc ccacgagctg gtggagcgcg gcttcaaggt cgacgtctac gagccggtcg
     1441 ccctcggcgg caaggcacgc tcgatcgacg tgcccgggag cggcaagggc cggcgccggc
     1501 cgctgcccgg cgagcacggc ttccgcttct tcccgggctt ctaccaccac atcccggact
     1561 cgatgcggcg cacgcccgac ggcaagaacg ccgagggcgt ctacgacaac ctggtcgaca
     1621 cctccgaggg ccggtcggta cgggccgacg gccgcgcgga cgcgcagctg ttcgggatga
     1681 tcccggaccc ggtcgcggcc acgacgcccc agggcctcca ggcgctcctg atcgaggaga
     1741 tcgtgaagat gaagatggtc ccgccgcacg aggcgggcta tctggcgtcg cgcctcgcgg
     1801 tgttcctgac cagcagcgag gagcgccggt acggccagtg ggagcacgtg gcgtggtggg
     1861 actacgtggg cgcctcgagc cgctccgagg agtaccagaa ggtcgccgcc cgcgggctga
     1921 cccgatcgct ggtggcggcc aaggagacgg tggcctcgac ccggaccatc ggcaacatgg
     1981 ccgaggcctt catcatgaac atcatgcagc gcggaaacga cggcgcgctg gaccgggtgc
     2041 tggacgcgcc caccaacgag gcctggatcc agccctgggt caagatgctc aggcgccggg
     2101 gcgtgcgctt ccacctcggc cagcgcgccg tggcgctgga cgtccgcgga gggcgggtgc
     2161 gctcggccgt ggtgcgcgac cggcgcggtc gccggcggcg cgtgagcgcc gactggttcg
     2221 tgtcggcgat gccggccgag cggatggtgc cgctgttgtc ccgccagctg ctgcgggccg
     2281 accccgccct ggagggcatc cgctcgctcc aggtcgactg gatggcgggg gtgcagtact
     2341 acctgcccaa gaagatcgac ctcacccacg ggcacgtgac ctacgtcgac tccccgtggg
     2401 cgctgacctc gctgacgcag gcgcagttct gggccgagcg cgacttcccg aagacgtacg
     2461 gcgacggcaa gaccgtcgac tgcctctccg tggacatctc cgactgggac tcgccggggc
     2521 tcctggtcaa gaagccggcc aagcggtgca cgccggcgga gatcaagcgc gaggtctggc
     2581 accagatcac cgagcacctc aaggacaccg acccggcgat cctgcagctg ggctcgcccc
     2641 gcgcgttcct ggaccccggg atcgtgtggg accggcggac ccgccgcaac cgcaacgcca
     2701 cgccgctgct ggtgaacacc gtcggatcgt gggagaagcg gccgaaggcg aagaccgcgg
     2761 tgtccaacct gttcctgtcc ggcgactacg tgcagaccga catcgacctg gcgaccatgg
     2821 agggggccaa cgagtcgggc cgcgcggcgg tggcggggct gctggacgcc gcggggtcca
     2881 gcgccgccaa gcccaagatg ttcaagctct acgacccacc cgagttcgag gccgccaagc
     2941 aggcggacaa ggccctgtac gcgcagggac tgccccacgt gctcgacggg cccggggcgc
     3001 ggtgaccgag cacgtcgacg cgatcgtggt cggcgcccgc tgcgcggggg ccgccaccgc
     3061 gaccgcgctg gcgcgggcgg ggcgccgggt ggtcgcgctc gagcgggcgc gcttcccggc
     3121 ggacaccctg tccacccacc tgctcttcct gggcggggtg ctggagctcc agcggctggg
     3181 cgccttggag cgggtgctgg agctggggcc gcccaagctg accgaggcgt cgatggggtg
     3241 ggggccgttc gaggccgagg ccggctacac gccggtgcag ggcatcgact acgcgctctg
     3301 cgtacgccgc ccggggctgg acgccgcgct ggtggccacc gcccgggacg ccggggcgca
     3361 ggtgcgcgag cagtcgaccg tgcgggaggt gcttgtcgag gacggccgcg ccgcgggcgt
     3421 gcgctaccgc gacgaggacg gcagcgagca cgagctctgc gccccgctcg tggtgggggc
     3481 cgacggtcgc cggtcggcgg tggcgcgggc gatgggcgcg gacgccccgg agctcgagag
     3541 ccccaacggc cgcgcgtgct tcttcgccta ctggcgcgac ggcggcggcg ggtcgcgctc
     3601 gctggcgtcg cagtggcgcc agggccgcga gctggtcacc gccttcccgt gcgacgacgg
     3661 cctggtgctg gtgctgctga tgcctccggt cgagcgggcc gaggacttcg ccgccgacct
     3721 ggcgggcgag tacgaccgca ccgtcgcctc cgtgccccag ctggccgagc ggctgcgcgg
     3781 ggccacgcgc cagagcaagg tgcgccacac caccaagacc gcctcctact tccgccgttc
     3841 gtcggggcct gggtgggcgc tgccgggcga cgcgggccac ttcaaggacc cggtgaccgc
     3901 gcagggcatc cgcgatgcga tgcgcttcgg ccgcctgctg ggcgagcagg ccgcaccggc
     3961 gctggacgac ccgcgccggc tggaccgggc gcttcgccgc tgggagcagc gccgcgaccg
     4021 cgagtgcctg cccatgtacg cgtggaccaa ccggctggcg cgcggcgagg agatgggcgc
     4081 gctggagatc gagctgtacc ggcgcggcgc cggcgacccg gagctgacgc ggcggttcct
     4141 ggacgtgatg gcgcgcaccc ggacccccgc gcaggccttc gggcccgcca cgaaggcgtc
     4201 gctcgcgctg gcggccgcgc gccgcccggg cggcggtcgc gccgttgcgc gggaggtcgg
     4261 ccgggagctg cgcgacgtgc tcgccgagcg cgtcgagcgg ggccggctgt ggtcggctac
     4321 gcggtggcgg gggcgccctg tgcgtccgcg tcgaacgcgg acgcggccgc tgccaggcgg
     4381 aacggctcca gctcgtcgcg ctggcgctgg gcctgctcgg ccagcgcggt gaagtcgaag
     4441 ctggtcagcc gcgggtcggt gcgctcggcc agcgcccgcc acagcaggcg cttgccctcg
     4501 atcccgatcg acagcccctc cagctcgacc atccggctca gcgccgagta gccggtcagc
     4561 tggtcgttcg gcttgagccg gccggccttc tcaccgaccc aggcggcggc caccttggcc
     4621 gggttcgggt gcgatccgac cgcctcgatc accgacttca gcgaggaccg ctcgccctcg
     4681 aactcgctcg cgaagcgcga gagcagggtc ccgagctcgg tgccctcgtt ggaccccgcg
     4741 gcgcggcggg ccagctcgag gcccgccgtg gagccggcga gatggtcgtt cagatatgtg
     4801 ggcaggtggt cggacatggg cggtggctac ccggcggagc atttgacaag ccgggggatc
     4861 gcccctctga tggggccccg gcaggggtac cgatccatgg ggagattcac ttgatgcgtc
     4921 ttggcgtccg tctgaacgtt gtcctgatcc tggcgctgtc gtgcgcgctg accgccggtg
     4981 tgggggtggc gggagcggat ggctcgggtg ccgcgccggg cgcggaggcg accgccaaga
     5041 agaagaagtg cggcaagggc aagacgcgcg tcaagcgcaa gggccgcagc agcgtgtgcg
     5101 ccaccaagtg caaggcgggc ttcaagcaca acgtgtccaa gcggggcaag gtcacctgcg
     5161 gccgcaagcc gggctcgaag cagcgcacgc cccagggcgg tagcggcccg aaggccggca
     5221 actacaccgg cacgaccgcg cagaaccgca ccatgacctt caccgtggcc ggcgggcagg
     5281 tcaccaactt ccaggcgggc gtgaacacct actgctccac gccgggcaac agccggttcc
     5341 agatcgacgc catcgccaac atcccgccga tggccatcgg gccggacggt tccttcagct
     5401 acaagggcac ctccgaccag ggcaaccccg agatcgccgg caagatcacc ggcgggagcg
     5461 cgacggggac ggtgggcatg agccggggcg actccaacta cagcggcggc cagctcacgt
     5521 tcggcagctg ctccgcgtcc ggcgtgacct ggaacgcggc cggctgagcg ccggcccggc
     5581 tcaggccagg ccggcgtacc agagcccgaa cagcacgccg aaggtgccca gggcggggat
     5641 gacgacggtg cggtagaggg gctcgacccg ctcccgggtc agcgtccagg cgaccccgcc
     5701 gctgcagagg gccatcgaca ccgcggacat gggggcgaac accgccagcg cggccaccgc
     5761 ctgtccgcgg gtgggcagcg ccgcgatcag cagcaggacg accgcgccgg tcccggcgag
     5821 cccgtggagg accccgatgc cgagggcctg gcgcggcgag cggacggtgg tgcgatggcc
     5881 ccgcgcccac cggaagagca tccgcagcgc cagcacgacg atcaccgcgc cgaccgcccg
     5941 ctccgcgccg ctctcgagcc agggcggcag cccggactcc agcacgatca gcggcgcgcc
     6001 gaccatcagc agggtcgcgg cgtggcccgc tccccaccag gcgcccaatc gcgcagcggc
     6061 gcgcgtgtcc gcgtcctcgg aggccatcag cgacgtcacc gccagcacgt ggtcggggtc
     6121 ggaggcgtgc cggagaccca gcagcagggc gatcagcagg gccaccagca gcggctggcc
     6181 ctcgaacagg gcggtgagcc acccgtcggc gtgctccacc ggcccgaaga gccactcgaa
     6241 gcccatgggt tgagtatcgt caatcgcgtg agcgaactgg cgcccgatta cttcaccaag
     6301 cacctggccg acgtcgatcc ggagatcgcg caggtggtct ccgacgagga gcgccgccag
     6361 gaggcgacgc tggagatgat cgcctcggag aacttcgtcc cgcaggcgat cctggaggcg
     6421 cagggctcgg tcgccaccaa caagtacgcc gagggctacc ccggccgtcg ctactacggc
     6481 ggctgcgagc acgtggacgt ggcggagcag ctcgcgatcg accgcgccaa ggcgctgttc
     6541 ggcgccgagc acgccaacgt ccagccgcac gccggcgcgc aggccaacac ggccgtctac
     6601 cacgcgctgc tgcagccggg ggacacgatc atgggcctgt cgctgcccca cggcggccac
     6661 ctcagccacg gcatgaagat caacttctcc gggcggctct acgacatcgt cgcctacggc
     6721 gtccgcgagg aggactcgcg actcgacatg gacgagctcg cggagatcgt ggcggatgcc
     6781 aggccgaagc tcatcctggc cggctggtcg gcatat
//
LOCUS       DASUPW010000497         6852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_668719, whole genome shotgun sequence.
ACCESSION   DASUPW010000497 DASUPW010000000
VERSION     DASUPW010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6852
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668719"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VFY44_11105"
     CDS             complement(<1..909)
                     /locus_tag="VFY44_11105"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX5911036.1"
                     /translation="MEKFIIEGGQPLSGTVVPAGNKNAALPALAATLLTEEEVVLRNV
                     PRIRDVEAMIELLRSVGATAEWRGDNVVSVNAAAATTAELDTELTERIRASFLLAGPL
                     LARFGSALMPPPGGDVIGRRRLDPHLDAFRALGATVEGRKSYAITAPQGGLRACDFFM
                     DEPSVMATENALMAAALTPGSTVIHNAASEPHVQDLARLLLAMGARIEGIGSNVLVVH
                     GEASLGGADYAIGPDYIEVGSFIALAACTGGELRIKDCVADDLRMTRLAFERLGCKIE
                     YDGADVIVPPGQALRIKDDEGDAVSKI"
     gene            1016..1597
                     /locus_tag="VFY44_11110"
     CDS             1016..1597
                     /locus_tag="VFY44_11110"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX5911037.1"
                     /translation="MPSPTRDEAWDLFCEWTESESLRKHVLGVEAAMVAYAREYGEDE
                     ELWAATGILHDLDYEKHPDLETGHPRVALELFEELDYPRELIDAVAGHAPYLGVPRET
                     RLAKTLYAVDELSGFVAACALVRPTGIEGMTPKSVKKKLKTPAFAAAVNRDEVREAAA
                     ELGVDFDEHLRTVIGAMEDRKDQLGLAAPPPSG"
     gene            complement(1565..1819)
                     /locus_tag="VFY44_11115"
     CDS             complement(1565..1819)
                     /locus_tag="VFY44_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911038.1"
                     /translation="MADHIPDRCARCNTALDPGDCFVVEGTGLCRLEHIVAWVLRGGA
                     PEGPLTVTRHRMGHEIVDEFGSAEELRAWASAGGRWGGQA"
     gene            1820..2893
                     /locus_tag="VFY44_11120"
     CDS             1820..2893
                     /locus_tag="VFY44_11120"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX5911039.1"
                     /translation="MADLRIIVLEGDETGQELLEQSLRLLDADVIGLEARLERFDLSL
                     DNRRATENGVVEDAAEAMRAAKLSIKAATVTPEGKDDVGSPNRILREALDGQVIIRTG
                     RRIPGITPVAGIHHPIAVVRMAVGDAYGAEQGREEVDGEEFAWRTERISRSVCRAVAE
                     VSFRTAERLGGQVYGGPKWTVSPVYEGMLKEEMDDAAERHPDVRYSPVLIDATYAGLV
                     GSRPDAPLVIPALNRDGDCLSDLVLPLFGSIAGAESVLLALDEDYEPSAVMAEAPHGT
                     APGLLGKDVANPMAMILAVAALLEYADELGHPGTRPASEAIYAAVLDAVDAGVSTPDL
                     GGHASSTEFTDAVIDRVKSSLSS"
     gene            complement(2871..3284)
                     /locus_tag="VFY44_11125"
     CDS             complement(2871..3284)
                     /locus_tag="VFY44_11125"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX5911040.1"
                     /translation="MSEPFRHSVRVRYSECDLQGIVFNANYLTYVDDALTELWRAAVP
                     GGYTGMVESGVDMVVGEANIRYLAPARVDDLLDLDVSVARLGTTGMTLEVAIGCEGRA
                     LTEVTMRYVFVAAGEGGKVPIPDAVRDVLSSKVTT"
     gene            3353..4777
                     /locus_tag="VFY44_11130"
     CDS             3353..4777
                     /locus_tag="VFY44_11130"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEX5911041.1"
                     /translation="MRSRPLISLLVALAAVLLVPAQASAGARMETSIMDDQLLLNASP
                     AALESDMALFKRLGVDRLRVSAFWNQVSPTPDARTKPAGFDLRDHTSGLYNFAELDRV
                     VDSAVRNGLRVMISISTPAPVWASADPSRGNQLWKPRADLFGEFTDAVVRRYADRVDH
                     WGISNEPNQGVWLQPQSDRSGLVAPHLYRSLVMAAYPRIKALDPTSTALVGELAASGR
                     SGRGATRPIRPLLFLREMACRDSRWRPIRRGRCKNFKPIPADALGHHPYNLLQRPTNA
                     SPNKYDAAIGDGRRLTRTLDRLVRLRALQPGRGRRLSVFYTEFGYQTTPPDPFAGVSL
                     NQQRLYLQQAAFIAQRTPRVRGLNQFRLTDGAIAGKGLRRFQEFQSGLMFRNRKPKPA
                     YSVFAHPFVISGDRFWGQVRPGTGHTVRVQRKPTARGSWRLVAQVPTDRLGYFTFRLR
                     GRKPGYYRYLYEGGKRSGTVRVRR"
     gene            4861..5070
                     /locus_tag="VFY44_11135"
     CDS             4861..5070
                     /locus_tag="VFY44_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911042.1"
                     /translation="MRQKIGTALVDRIDLLVEFTTLGEYALADDLRPVAIHAETPAPS
                     ARRSRDDCPWRDAPLSRRCDGSARS"
     gene            complement(5036..5515)
                     /locus_tag="VFY44_11140"
     CDS             complement(5036..5515)
                     /locus_tag="VFY44_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX5911043.1"
                     /translation="MLLEGWAWGRPRSGHPEGSVGAHVADLLETIEEWGETGTRRQEL
                     RLLALIHDALKFRVNHARRRTGENHHAMRARRFAEGYLDDERLLAALELHDRPYGIWR
                     KQRRTGRFDQERFDDMVARIPDMALFERFVELDASTEGKDPAPLEWFRNELSRRSAG"
     gene            complement(5587..5970)
                     /locus_tag="VFY44_11145"
     CDS             complement(5587..5970)
                     /locus_tag="VFY44_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911044.1"
                     /translation="MSTLTIVLIVVAVIVIALFVGGLVAARKRDREQAPAYGEHLREA
                     DQHLERARAADRGWDPALLETAAREALGRSHPGTEFDQLVLVLVDDQPGVHEDRAHYE
                     AHAGEERVAVVLTRDEQGWRGQTAT"
     gene            complement(6039..6155)
                     /gene="rrf"
                     /locus_tag="VFY44_11150"
     rRNA            complement(6039..6155)
                     /gene="rrf"
                     /locus_tag="VFY44_11150"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(6257..>6852)
                     /locus_tag="VFY44_11155"
     rRNA            complement(6257..>6852)
                     /locus_tag="VFY44_11155"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1011 a         2516 c         2313 g         1012 t
ORIGIN      
        1 gatcttggac acggcatcgc cctcgtcgtc cttgatccgc agcgcctgcc ccgggggcac
       61 gatcacgtcg gcgccgtcgt actcgatctt gcagcccagg cgctcgaagg ccagccgggt
      121 catgcgcagg tcgtcggcca cgcagtcctt gatccgcagc tcgccgccgg tgcaggccgc
      181 gagcgcgatg aagctgccca cctcgatgta gtccggcccg atggcgtagt ccgccccgcc
      241 cagggaggcc tcgccgtgca cgaccaggac gttggagccg atgccctcga tccgggcgcc
      301 catggccagc agcagccgcg ccaggtcctg gacgtgcggc tcggaggccg cgttgtggat
      361 caccgtggat ccgggcgtca gcgcggcggc catcagcgcg ttctcggtgg ccatgaccga
      421 tggctcgtcc atgaagaagt cgcaggcgcg cagcccgccc tggggcgcgg tgatggcgta
      481 ggacttgcgg ccctcgacgg tggcgcccag cgcccggaag gcatccaggt gcgggtccag
      541 gcgccggcgg ccgatcacgt cgccgccggg gggcggcatc agggccgacc cgaagcgggc
      601 cagcagcggg ccggccagca ggaacgaggc gcggatgcgc tcggtcagct cggtgtccag
      661 ctccgccgtg gtggcggcgg ccgcgttcac ggacaccacg ttgtcgccgc gccactcggc
      721 cgtggccccc acgctgcgca ggagctcgat catggcctcg acgtcgcgga tccgcggcac
      781 gttgcgcagc acgacctcct cctcggtgag gagcgtggcc gcaagcgcgg gcagggcggc
      841 gttcttgttg ccggcgggca ccacggtgcc cgacagcggc tggccgcctt cgatgatgaa
      901 tttctccatg cgtgggtccg ccgaggccgg ggacagaagc tcaggcggct gcgcaagggt
      961 agcgaagggc atctcgctgg cgaagttcgt ttcgacccgt cgataccctg cccgcgtgcc
     1021 ctccccgacc cgcgacgagg cctgggacct gttctgcgaa tggacggagt ccgaatcgct
     1081 gcgcaagcac gtcctggggg tcgaggccgc gatggtcgcc tacgcgcgcg agtacggcga
     1141 ggacgaggag ctctgggccg ccaccggcat cctccacgac ctggactacg agaagcaccc
     1201 ggacctggag accggccacc cgcgcgtggc cctggagctg ttcgaggagc tggactaccc
     1261 ccgggagctg atcgacgcgg tcgccggcca cgcgccctac ctgggcgtcc cgcgcgagac
     1321 ccggctggcc aagaccctct acgccgtcga cgagctcagc ggcttcgtgg ccgcgtgcgc
     1381 gctggtgcgc cccaccggca tcgagggcat gaccccgaaa tcggtcaaga agaagctcaa
     1441 gacgccggcg ttcgccgccg cggtgaaccg cgacgaggtg cgcgaggcgg ccgccgagtt
     1501 gggcgtggac ttcgacgagc acctgcggac ggtgatcggg gcgatggagg accgcaagga
     1561 ccagctaggc ctggccgccc caccgccctc cggctgacgc ccacgcgcgc agctcctcgg
     1621 cggagccgaa ctcgtcgacg atctcgtgcc ccatccggtg gcgcgtgacg gtgagcggcc
     1681 cctcgggcgc gccgccgcgc agcacccagg ccacgatgtg ctccagccgg cacagcccgg
     1741 ttccctcgac cacgaagcag tcgcccgggt ccagcgcggt gttgcagcgg gcgcagcggt
     1801 cggggatatg gtcagccacg tggcagacct tcgcatcatc gtcctggaag gcgacgagac
     1861 cggccaggag ctgctggagc agtcgctgag gctgctggac gcggacgtga tcgggctgga
     1921 ggcgcgactc gagcgtttcg acctgtcgct ggacaaccga cgcgccaccg agaacggcgt
     1981 ggtggaggac gcggccgagg cgatgcgcgc ggccaagctg tcgatcaagg cggccaccgt
     2041 cacgccggag ggcaaggacg acgtgggctc gcccaaccgg atcctgcgcg aggcgctgga
     2101 cggccaggtg atcatccgca ccgggcgccg gatccccggc atcacgccgg tggcgggcat
     2161 ccaccacccg atcgcggtcg tgcgcatggc cgtcggcgac gcctacggcg ccgagcaggg
     2221 ccgcgaggag gtggacggcg aggagttcgc ctggcgcacc gagcgcatct cgcgcagcgt
     2281 ctgccgggcc gtggccgagg tcagcttccg caccgccgag cgactgggcg gccaggtcta
     2341 cggcggtccg aagtggacgg tcagcccggt ctacgagggc atgctcaagg aggagatgga
     2401 cgacgcggcc gagcgccatc cggacgtgag gtactcaccg gtcctgatcg acgcgaccta
     2461 cgccgggctg gtgggctcgc ggcccgacgc gccgctggtg atccccgcgc tgaaccgcga
     2521 cggcgactgc ctgtccgacc tggtgctgcc gctgttcggc tccatcgccg gcgccgagtc
     2581 ggtgctgctg gccctggacg aggactacga gcccagcgcc gtgatggccg aggcgcccca
     2641 cgggaccgct ccgggcctgc tgggcaagga cgtggccaac ccgatggcca tgatcctggc
     2701 ggtggccgcg ctgctggagt acgccgacga gctgggacac cccggcaccc ggcccgcgtc
     2761 ggaggcgatc tacgcggcgg tgctggacgc ggtggacgcc ggcgtgagca cgccggacct
     2821 gggcgggcac gcctcctcga ccgagttcac cgacgcggtc atcgaccgcg tcaagtcgtc
     2881 actttcgagc tgagcacgtc ccggaccgcg tccgggatcg gcaccttgcc gccctccccc
     2941 gcggcgacga acacgtaccg catggtcacc tccgtcagcg cgcgcccctc gcacccgatg
     3001 gccacctcga gcgtcatccc ggtggtgccc agccgcgcca ccgagacatc caggtccagc
     3061 aggtcgtcca cgcgcgccgg cgccaggtac cggatgttgg cctcccccac gaccatgtcc
     3121 acgcccgact cgaccatccc cgtgtacccg cccgggaccg cggcccgcca cagctcggtg
     3181 agcgcgtcgt caacgtaggt caggtagttg gcgttgaaga cgatcccctg caggtcgcac
     3241 tcggagtagc ggacccgcac ggagtggcgg aatggttcgg acacgcctgc catcatgccc
     3301 ggctcgcgcg gttgcgcgac tttcgcccgc ccgcccggtt tccacctccg caatgcgctc
     3361 acgcccgctc atctccctgc tcgtggccct ggccgcggtc ctgctggtcc cggcgcaggc
     3421 gtccgcgggc gcgcgcatgg agacgtcgat catggacgac cagctgctgc tgaacgcctc
     3481 cccggccgcg ctggagagcg acatggcgct gttcaagcgc ctgggcgtgg accgcctgcg
     3541 cgtgtcggcg ttctggaacc aggtgtcccc cacgcccgat gcgcgcacga agcccgccgg
     3601 gttcgacctg cgcgaccaca ccagcgggct ctacaacttc gccgagctgg accgggtggt
     3661 cgattcggcg gtccgcaacg gcctccgggt gatgatctcg atctccacgc cggcgccggt
     3721 ctgggccagc gccgacccca gccggggcaa ccagctgtgg aagccgcggg ccgacctgtt
     3781 cggtgagttc accgacgccg tcgtccgccg ctacgcggac cgcgtcgacc actggggcat
     3841 ctccaacgag cccaaccagg gcgtttggct gcagccgcag agcgaccgtt cgggcctggt
     3901 ggccccgcac ctgtaccgga gcctcgtcat ggccgcctac ccccggatca aggccctgga
     3961 ccccacgtcc accgcgctgg tgggcgagct ggcggccagc ggccgctccg gccgcggcgc
     4021 gacccggccg atccggccgt tgctgttcct gcgcgagatg gcgtgccgcg attcgcgctg
     4081 gcgcccgatc cggcgcgggc ggtgcaagaa cttcaagccg atcccggccg acgccctggg
     4141 ccaccatccg tacaacctgc tccagcggcc caccaacgcg tcgccgaaca agtacgacgc
     4201 cgccatcggc gacggacgcc ggctgacgcg gacgctggac cgcctggtgc ggctgcgggc
     4261 gctccagccc ggccgcggcc gccgcctgag cgtcttctac accgagttcg gctaccagac
     4321 gaccccgccc gaccccttcg ccggcgtcag cctcaaccag cagcgcctgt acctccagca
     4381 ggcggcgttc atcgcccagc gcacgccgcg ggtgcgcggc ctgaaccagt tccgcctgac
     4441 cgacggcgcc atcgccggca agggcctgcg ccggttccag gagttccagt ccggcctgat
     4501 gttccgcaac cgcaagccca agccggcgta ctcggtcttc gcccacccgt tcgtgatcag
     4561 cggcgaccgc ttctggggcc aggtccggcc cggcaccggc cacaccgtcc gcgtccagcg
     4621 caagcccacg gcgcgcggct cgtggcgcct ggtcgcccag gtcccgacgg accggctggg
     4681 ctacttcacg ttccgcctcc gcgggcgcaa gccgggctac taccggtacc tgtacgaggg
     4741 cggaaagcgc tccggcaccg tccgcgtgcg gcgctagtcc ctgcttgcac gaaaccttgc
     4801 attccgaggg cccttccgtc cgccccccgg cctagcttgt gcgaacacat gttcccgcga
     4861 atgcgacaga agatcggcac cgcgctggtc gacaggatcg acctcctggt cgagttcacc
     4921 accctcggcg agtacgccct ggcggacgac ctccgccccg tggccatcca cgccgagacg
     4981 ccggcgccca gcgcccggcg cagccgcgac gactgcccgt ggcgcgacgc gccgctcagc
     5041 cggcgctgcg acggctcagc tcgttcctga accactccag cggtgcgggg tccttgccct
     5101 cggtggacgc gtccagctcc acgaagcgct cgaacagcgc catgtcgggg atccgggcca
     5161 ccatgtcgtc gaagcgctcc tggtcgaagc gaccggtgcg gcgctgcttg cgccagatgc
     5221 cgtagggccg gtcgtgcagc tccagggccg ccaggaggcg ctcgtcgtcc aggtacccct
     5281 cggcgaaccg tcgcgcgcgc atggcgtggt ggttctcccc cgtgcgccgg cgcgcgtggt
     5341 tgacgcggaa cttcagcgcg tcgtggatca gggccagcag ccgcagttcc tggcgccggg
     5401 tcccggtctc gccccactcc tcgatggtct ccagcaggtc ggccacgtgg gcgccgacgc
     5461 tgccctcggg gtgcccgctg cgcggacggc cccaggccca gccttccagc agcacgggat
     5521 cctcggccag gcgctcctcc agggccgtct cgggctcgaa gcccggtatc ggagtggcct
     5581 gcgggctcag gttgcggtct ggccgcgcca gccctgctcg tcgcgggtca gcacgacggc
     5641 cacccgctcc tcgccggcgt gtgcctcgta gtgggcgcgg tcctcgtgca cgccgggctg
     5701 gtcgtccacc aggaccagca ccagctggtc gaactcggtg cccgggtgcg accggccgag
     5761 cgcctcgcgc gcggcggtct cgagcaacgc cggatcccag ccgcggtcgg cggcgcgagc
     5821 ccgctccagg tgctggtcgg cctcgcgcag gtgctcgccg tacgccggcg cctgctcgcg
     5881 atcacgcttt cgcgcggcca ccaggccgcc tacgaacaag gcgatcacga tgaccgccac
     5941 gactatgagc acgatcgtga gcgttgacat ggtgagttcg gatgctgaaa gacaacgggc
     6001 cgcgccgcct agccggcggt acggcccgtt gatgaagtaa acggcggcga cctactctcc
     6061 caggcccttt caggccaagt accatcggcg cggagaggct taactgctct gttcggaatg
     6121 ggaagaggtg tttccccctc gctagtgcca ccgaaagatt gcgagaccag ctctcgccgg
     6181 gccctcaaaa ccgcacagtt gaccttgggt accacgttag aactgcgcgt gaccgcgaac
     6241 gatcacgcaa gctaaaaccg tcaagccctc gaagcattag taccggtctg ctgagcacat
     6301 cactgtgctt gtacatccgg cctatcacct ggtggtctgc cagggctctt actcgcttga
     6361 agcgatggga gaactcatct cgaggtcggc ttcccgctta gatgctttca gcggttatcc
     6421 gatccagacg tagctaacca gccatgccct tggcaggaca actggtacac cagaggtctg
     6481 ttcatcccgg tcctctcgta ctagggacga ctcctctcaa ttctccaacg cccacggtag
     6541 atagggaccg aactgtctca cgacgttctg aacccagctc gcgtgccgct ttaatgggcg
     6601 aacagcccaa cccttgggac ctacttcggc cccaggatgc gacgagccga catcgaggtg
     6661 ccaaaccggg ccgtcgatgt ggactcttgg gcccgataag cctgttatcc ccggagtacc
     6721 ttttatccgt tgagcgacgg cgcttccact tgcaaccgcc ggatcactaa gcccaacttt
     6781 cgttcctgct ccacccgtcg gtgtcacagt caagctccct ttatgccttt acactctacg
     6841 cgcgatttcc ga
//
LOCUS       DASUPW010000498         8386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_946269, whole genome shotgun sequence.
ACCESSION   DASUPW010000498 DASUPW010000000
VERSION     DASUPW010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8386
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946269"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1867
                     /locus_tag="VFY44_11160"
     CDS             <1..1867
                     /locus_tag="VFY44_11160"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX5911045.1"
                     /translation="AVASSLYYVLGIVAGDAQGLTPLVFAVGALFFALTVLTYVEGSS
                     LHPERGGASVLARYAFNELWSFIAGWAILLDFLIVLAIGAVAVPHYLAAFWGPADEDA
                     VAILISGATIGWVAWMNFQGVSAQRFKLVLRLSLLSLVVFAAIVVVGAIEVFDASLIL
                     DSIDLGTRPELDDLVFAMVVATVACTGIEAASGLAADLKLRRSQLRRVAVVLAGSVLV
                     LYVSVSVIALMAVPVGAGGTELGTTYLDAPLLGVVSAFDPAGLGELSRYVVGLVGALV
                     LFQAVNGQMLGIGRLAYSLGTHRQIPSLLSRLDSRRSTPYVTIALAAVIGFGLALTGD
                     IDFLAGIFSFGAMLAFTLAHLSVIVLRFREPDRTRPFRIPLSVPVGGGSIPLPAALGA
                     LMSGAGWVTVVVLHEGARIAGGVWMLAGVALYVVYRRSQGKALTKRFTIPEEALKEAK
                     EVEYGSILVPVFGGDLDDDIVGTAGRLAADEAEEGEGGAMLEAVYVFEIPMSLPIDAR
                     VSPDKGAAGRKALARAKEVGEEYEGVEVATATVRGRSAGAAIVSEAKRRGVEAIVLAA
                     EPPSRIRGGVRLGGKASIEDRFAGDTTRYVVEKAPCKVILTAPAADVPAPAQTAS"
     gene            1895..2341
                     /locus_tag="VFY44_11165"
     CDS             1895..2341
                     /locus_tag="VFY44_11165"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX5911046.1"
                     /translation="MFVLIVGCGRVGSSVAKSMLSEGHDVSVLDENPEAIALLNRGQE
                     QSWEDLGGSFTVGTALEIDALLAAGIENADAFVASTDGDNTNLVIAQIAQRRFEIGKV
                     VVRVLDPGRAKWYAEQGLQTVCPTQTAIDLLERAVRGEPERRGTTV"
     gene            2341..3015
                     /locus_tag="VFY44_11170"
     CDS             2341..3015
                     /locus_tag="VFY44_11170"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX5911047.1"
                     /translation="MYVLIVGAGKVGWNLARELIDKQNEVTVVEASRRRYEVVEEELE
                     HNIQYGDGTELWILERAGIERADLVIAVTGDDEDNILICQVAKEKYGVERVVARCNNP
                     RNLQHFELLGVKPAVSATDLIMRLIEHEVPKYGLVHLLDLKEERLEIIEMEVGEGSPA
                     DGSEVQELGLPQGALVISILRDGGGFVPTGDSVIKAGDEVLLVLDIGLEESVTIRFAG
                     STAAAG"
     gene            3046..3116
                     /locus_tag="VFY44_11175"
     tRNA            3046..3116
                     /locus_tag="VFY44_11175"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3078..3080,aa:Gln,seq:ctg)
     gene            3185..3793
                     /locus_tag="VFY44_11180"
     CDS             3185..3793
                     /locus_tag="VFY44_11180"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5911048.1"
                     /translation="MGRSDGSDRYRQRLINGLASALAEKELGATTVADIVRHAHVSKR
                     TFYEHFADKRECFAALAMSITEQMLGVIDQAVAQEDTWEAKARAACLTYMRVAASSPQ
                     LTRTLVLEVQAGGPQALQVRRRAYKAHADTFVRLSEDAAKERPDVRPISPAVATAIVA
                     GINELMLEVVEEGDASRLTEVVDAAFEIIYAVAVTAPPAAEA"
     gene            complement(3790..5568)
                     /locus_tag="VFY44_11185"
     CDS             complement(3790..5568)
                     /locus_tag="VFY44_11185"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5911049.1"
                     /translation="MSARSNWATVRSERGRARRLGKVLALLRPYRGRVILMLITLVVA
                     VAAALAPAYLAGRAIDAGIRDKDTAALTWIMIAFLGAAVVNFGATYAQTYLTSWVGQR
                     ALQDLRRQIFQHLQKLSIGFYSRNKSGVLISRMTNDVQALDQLVTDGIVTLFSASLTL
                     LGTAAILLALDVKLALITFAVFPVLGIASFAFRVASADAYRATREKIASITSYLQETL
                     SGVRIVRAFGQEPRHERQFTGLNDENRDANMKTVYLNAAYFPSVELLSAVATAVILVF
                     GGNEVINGTVSIGVLASFVFYLSTFFDPIQQLSQLYTTYQSGMAALDKIFGLLDEEPE
                     LEDAPDAVDLPQVRGQIDFEGVDFTYGGGDDVLHGIDLRVPPGQTVALVGATGAGKST
                     LAKLVARFYDPTGGRITIDGHDLRKVTSHSLRSQLGTVPQESFLFSGTIRDNIAFGAP
                     GATDDDVRAAARAVGATEFIEKLPDGYDTEVGERGGHLSAGQRQLVAFARAAAADPRV
                     LILDEATSNVDVATEARIEHGLRRLLAGRTAIVIAHRLSTIQDAGRIVVLEHGRIAEQ
                     GTHDELLAAGGAYAALYEDWARQTAA"
     gene            complement(5565..7328)
                     /locus_tag="VFY44_11190"
     CDS             complement(5565..7328)
                     /locus_tag="VFY44_11190"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5911050.1"
                     /translation="MKVFRRLLGFLRPYRAGVIASLLLAAAAMGTGVLLPFLIGRTID
                     DIRQGGDSLELLGWVLVAAGVLRLAFSVVRRLVAGRVSLGVEFDLRNRMYRHLQSLEL
                     GFFDGQQTGQLMSRSTVDLQSVRFFLGYGLIFMMQALLTIVIAAIVMIAVNPILAAVA
                     LAPMPLVIWAAARYGRLNRPASQEVQQRIAELTAEAEENVGGVRVVKAFAQETRQVHR
                     FNQAVTRVFDQSMVSTRLRAFYNPFIGFLPQLGLAAILLVGGRQAINGTISVGDFVAF
                     YGYVVMLTGPMRMLGVSLGMAQRAVASGNRVFEILDREPRLTAPEGAEPLPEGGGRVQ
                     MRGVSFGYDDGTEVLRDVDLDVPAGGTVAVVGATGSGKSTLVMLIPRLYDVDQGEVLI
                     DGVDVRDVDLASLRHEIAFVSDDAFLFSATLRENIAYARPEATDAEVEEAAHRAGLTE
                     VVADLPEGYDTLVGERGLTLSGGQRQRVAIARALLAGPRVLILDDATSSVDATTESRI
                     KEALGEVMEGRTTFVIAHRMSTISLADQVIVLEDGRIAAQGSHEELVEQSDLYRQIAQ
                     RGAPDPQLVAAADQGREAAGL"
     gene            complement(7393..7848)
                     /locus_tag="VFY44_11195"
     CDS             complement(7393..7848)
                     /locus_tag="VFY44_11195"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5911051.1"
                     /translation="MKVHVLQREQLLPHPVDRVFPFFAEARNLERITPPLLGFAVTNQ
                     EPIEMRAGAIIQYRLKLHGLPVDWLTRIDEWEPGVRFVDQQLVGPYRLWHHTHTFEAR
                     GGSTLMRDTVRYALPLWPLGELAMPLVRRDLKQIFDFRRDSVGALLDRD"
     gene            complement(7856..8053)
                     /locus_tag="VFY44_11200"
     CDS             complement(7856..8053)
                     /locus_tag="VFY44_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911052.1"
                     /translation="MVPLTIIAFFAGMIGLGLVVIVVETWWVVALVGITHMVASIVLG
                     WLVWKGLDDSPDGDPEAVAGP"
     gene            8187..>8386
                     /locus_tag="VFY44_11205"
     CDS             8187..>8386
                     /locus_tag="VFY44_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911053.1"
                     /translation="MALVAGILFALILAWRFAADDPASAIGAIYVVPVAFLAVHLGVR
                     GGMTGAAVAIGLSALWSEVQGVA"
BASE COUNT         1202 a         2920 c         3040 g         1224 t
ORIGIN      
        1 cgcggtggcg tcctcgctgt actacgtgct gggcatcgtg gcgggggacg cccaggggct
       61 gacgcccctg gtgttcgccg tcggcgcgct gttcttcgcc ctgaccgtgc tcacgtacgt
      121 ggagggcagc tcgctgcacc ccgagcgtgg cggcgcgtcc gtgctggccc ggtacgcatt
      181 caacgagctg tggagcttca tcgccggctg ggcgatcctc ctggacttcc tgatcgtgct
      241 ggccatcggc gccgtggccg tcccgcacta cctggccgcc ttctgggggc ccgcggacga
      301 ggacgcggtg gccatcctga tctcgggcgc cacgatcggg tgggtcgcct ggatgaactt
      361 ccagggggtc tcggcccagc gcttcaagct ggtgctgcgc ctgtccctgc tcagcctggt
      421 ggtgttcgcg gccatcgtgg tcgtgggcgc gatcgaggtc ttcgacgcgt cgctgatcct
      481 ggactcgatc gacctgggca cccggcccga gctggacgac ctggtgttcg ccatggtcgt
      541 ggcgacggtc gcctgtaccg gcatcgaggc ggcgtcgggg ctggcggccg acctgaagct
      601 gcgccggtcc cagctgaggc gggtggccgt ggtcctggcc gggtccgtgc tggtgctgta
      661 cgtgtcggtc tcggtgatcg ccctgatggc ggtccccgtc ggggccggcg gcaccgagct
      721 gggcaccacc tacctcgacg cgccgctgct gggggtcgtg tcggcgttcg acccggcggg
      781 gctgggcgag ctgtcgcggt acgtggtcgg cctggtggga gcgctggtgc tgttccaggc
      841 ggtcaacggg cagatgctgg gcatcggccg gctggcgtac tccctgggca cccaccgcca
      901 gataccgagc ctgctgtcgc ggctggactc acgccgctcg acgccgtacg tcacgatcgc
      961 cctggccgcg gtgatcggct tcgggctggc gctgaccggc gacatcgact tcctggccgg
     1021 catcttctcc ttcggcgcga tgctggcctt cacgctggcc cacctgtcgg tgatcgtcct
     1081 gcggttccgc gagcccgatc gcacgcgccc gttccggatc ccgctgagcg tgccggtggg
     1141 cgggggctcg atcccgcttc cggccgcgct gggcgcgctg atgagcggcg ccggctgggt
     1201 cacggtcgtg gtgctgcacg agggcgcccg gatcgccgga ggcgtgtgga tgctggccgg
     1261 cgtcgcgctc tacgtcgtct accggcgcag ccagggcaag gcgctgacca agcgcttcac
     1321 catccccgag gaggcgctga aggaggccaa ggaggtcgag tacggcagca tcctggtgcc
     1381 ggtgttcggc ggcgacctgg acgacgacat cgtgggcacc gcgggccggc tggcggccga
     1441 cgaggccgag gaaggggagg ggggagcgat gctcgaggcc gtctacgtgt tcgagatccc
     1501 catgtcgctg cccatcgacg cccgcgtgtc gcccgacaag ggggccgccg ggcgcaaggc
     1561 gctggcccgg gcgaaggagg tgggcgagga gtacgagggc gtcgaggtgg ccaccgcgac
     1621 cgtgcgcggg cggtccgccg gcgcggccat cgtctcggag gccaagcggc gcggggtgga
     1681 ggccatcgtg ctggccgcgg agccgcccag ccgcatccgc ggcggagtgc gcctgggggg
     1741 caaggccagc atcgaggacc gcttcgcggg cgacaccacg cgatacgtgg tcgagaaggc
     1801 gccgtgcaag gtcatcctga ccgcaccggc cgccgatgta cccgcgcccg cccagaccgc
     1861 atcctgaccc cttcccggcg ctattgtgcg cctcgtgttc gtcctgatcg tcggctgcgg
     1921 ccgagttggc tcctccgtcg cgaagtccat gttgtccgag gggcacgacg tctcggtgct
     1981 ggacgagaac cccgaggcca tcgcgctgct caaccgcggc caggagcaga gctgggagga
     2041 cctgggcgga tccttcaccg tggggaccgc gctcgagatc gacgccctcc tggccgccgg
     2101 gatcgagaac gccgacgcgt tcgtggcctc gaccgacggc gacaacacga acctggtgat
     2161 cgcccagatc gcccagcggc ggttcgagat cgggaaggtc gtggtgcgcg tgctcgaccc
     2221 cgggcgcgcc aagtggtacg ccgagcaggg cctgcagacc gtctgtccca cccagaccgc
     2281 catcgacctg ctggagcgcg ccgtccgcgg cgagcccgaa cggcgcggga cgaccgtctg
     2341 atgtacgtcc tgatcgtggg cgccggcaag gtgggctgga acctggcccg cgagctgatc
     2401 gacaagcaga acgaggtcac ggtcgtggag gccagccgcc gccgctacga ggtggtcgag
     2461 gaggagctcg agcacaacat ccagtacggc gacggcaccg agctgtggat cctcgagcgc
     2521 gccgggatcg agcgcgccga cctggtcatc gcggtcaccg gcgacgacga ggacaacatc
     2581 ctgatctgcc aggtcgccaa ggagaagtac ggggtcgagc gcgtcgtggc ccgctgcaac
     2641 aacccgcgca acctccagca cttcgagctg ctcggggtca agcccgccgt ctcggccacc
     2701 gacctgatca tgcgcctgat cgagcacgag gtgcccaagt acggcctggt gcacctgctg
     2761 gacctcaagg aggagcggct ggagatcatc gagatggagg tcggcgaggg ctcgccggcc
     2821 gacggctccg aggtgcagga gctggggctg ccccagggcg cgctggtcat ctcgatcctg
     2881 cgcgacggcg gcggcttcgt gcccaccggc gactcggtca tcaaggccgg ggacgaggtc
     2941 ctgctggtgc tggacatcgg cctcgaggag tcggtgacca tccgcttcgc cggcagcacc
     3001 gccgctgccg gataggccga tatcgttatc ctggctggtt cccgatgggg gatcgtctaa
     3061 cggcaagacg cctggttctg gtccaggtaa tcggggttcg aatccctgtc ccccagtaac
     3121 gcccctcgcc ccggcgtcta gtctttcccc atcaatgtca gccggggcag ggactgagga
     3181 gcgagtgggg cgatccgacg gttcggaccg ctaccgccag cggttgatca acgggctggc
     3241 ctcggcgctc gccgagaagg agctcggtgc caccaccgtc gcggacatcg tgcggcacgc
     3301 gcacgtgtcc aagcgcacct tctacgagca cttcgccgac aagcgcgagt gcttcgccgc
     3361 gctggccatg tccatcaccg agcagatgct cggcgtgatc gaccaggccg tggcccagga
     3421 ggacacctgg gaggccaagg cccgggccgc gtgcctgacc tacatgcgcg tggccgcgtc
     3481 cagcccccag ctgacgcgca cgctggtgct ggaggtgcag gccggcgggc cgcaggcgct
     3541 ccaggtccgc aggcgcgcct acaaggccca cgccgacacc ttcgtccgcc tgtcggagga
     3601 cgcggcgaag gagcgcccgg acgtgcggcc catcagcccc gccgtggcca cggcgatcgt
     3661 ggccgggatc aacgagctga tgctcgaggt ggtcgaggag ggcgacgcgt cgcgcctgac
     3721 cgaggtcgtg gacgccgcct tcgagatcat ctacgccgtg gccgtcacgg cgccgcctgc
     3781 cgccgaggcc taagcggcgg tctgccgcgc ccagtcctcg tacagcgccg cgtaggcgcc
     3841 gcccgcggcc agcagctcgt cgtgcgtgcc ctgctcggcg atccggccgt gctcgaggac
     3901 cacgatccgc ccggcgtcct ggatcgtgga cagccggtgc gcgatcacga tggccgtgcg
     3961 gccggccagc agccggcgca ggccgtgctc gatgcgggcc tcggtggcca cgtccacgtt
     4021 cgaggtcgcc tcgtccagga tcagcacccg tgggtccgcg gccgcggcgc gggcgaaggc
     4081 gaccagctgg cgctggcccg cggacaggtg cccgccgcgc tcgcccacct cggtgtcgta
     4141 gccgtcgggc agcttctcga tgaactcggt ggcgcccacg gcgcgggcgg cggcccggac
     4201 gtcgtcgtcg gtggcgcccg gcgcgccgaa ggcgatgttg tcgcggatcg tgcccgagaa
     4261 caggaagctc tcctggggca cggtgcccag ctgtgagcgc agggagtggc tggtcacctt
     4321 gcgcaggtcg tggccgtcga tggtgatccg gccgccggtg gggtcgtaga agcgcgccac
     4381 cagcttggcc agcgtggact tgccggcgcc ggtggcgccc accagcgcca ccgtctggcc
     4441 cggcggcacc cgcaggtcga tcccgtggag gacgtcgtcg ccgccgccgt aggtgaagtc
     4501 cacgccctcg aagtcgatct gcccgcgcac ctggggcagg tcgacggcgt cgggagcgtc
     4561 ctcgagctcg ggctcctcgt ccagcagccc gaagatcttg tccagcgccg ccatgcccga
     4621 ctggtacgtg gtgtacagct gggacagctg ctggatcggg tcgaagaagg tggacaggta
     4681 gaacacgaac gaggccagca cgccgatgga caccgttccg ttgatgacct cgttgccccc
     4741 gaacaccagg atcaccgcgg tggccacggc cgagagcagc tccaccgacg ggaagtaggc
     4801 ggcgttcagg tagaccgtct tcatgttggc gtcgcggttc tcgtcgttca ggccggtgaa
     4861 ctggcgctcg tgacgcggct cctggccgaa cgcgcgcacg atccgcacgc ccgagagcgt
     4921 ctcctgcagg tacgaggtga tcgacgcgat cttctccctc gtggcgcggt aggcgtccgc
     4981 cgaggcgacc ctgaaggcga agctggcgat gcccagcacc gggaacacgg cgaaggtgat
     5041 cagggccagc ttcacgtcca gcgccagcag gatcgcggcg gtgcccagca gcgtcagcga
     5101 ggccgagaac agcgtgacga tgccgtcggt gaccagctgg tccagcgcct gcacgtcgtt
     5161 ggtcatgcgc gagatcagca cccccgactt gttgcgcgag tagaagccga tcgacagctt
     5221 ctgcaggtgc tggaagatct gccggcgcag gtcctgcagg gcgcgctggc cgacccagct
     5281 ggtcaggtag gtctgggcgt aggtggcgcc gaagttcacc accgccgcgc ccaggaaggc
     5341 gatcatgatc cacgtcagcg ccgcggtgtc cttgtcgcgg atgccggcgt cgatggcgcg
     5401 cccggccagg taggcggggg ccagcgcggc cgcgacggcc accacgagcg tgatgagcat
     5461 gaggatcacg cgcccgcggt agggacgcag cagcgccagg accttgccca ggcgcctggc
     5521 gcgcccgcgc tcggagcgca ccgtcgccca gttggaccgc gcgctcacag gcccgccgcc
     5581 tcacggccct ggtcggccgc cgccaccagc tgggggtcgg gcgcgccgcg ctgggcgatc
     5641 tggcgataca ggtcggactg ctccaccagc tcctcgtggc tgccctgggc cgcgatccgg
     5701 ccgtcctcga gcacgatcac ctggtcggcc agcgagatcg tggacatgcg gtgggcgatc
     5761 acgaacgtcg tgcgcccctc catgacctcg ccgagcgcct ccttgatccg gctctcggtg
     5821 gtggcgtcca cgctggaggt ggcgtcgtcc aggatcagca cccgcgggcc cgccagcagc
     5881 gcccgggcga tggccacgcg ctggcgctgc ccgccggaca gcgtcaggcc gcgctcgccc
     5941 accagcgtgt cgtagccctc gggcaggtcg gccacgacct cggtcaggcc ggcgcggtgg
     6001 gcggcctcct cgacctcggc gtcggtggcc tcggggcggg cgtaggcgat gttctcgcgc
     6061 agggtcgcgc tgaacaggaa ggcgtcgtcg gacacgaagg cgatctcgtg gcggagggag
     6121 gccaggtcga cgtcgcggac gtcgacgccg tcgatcagca cctcgccctg gtccacgtcg
     6181 tagaggcggg ggatcagcat caccagcgtg ctcttgcccg acccggtggc gccgaccacc
     6241 gcgacggtgc cgcccgcggg gacgtccagg tcgacgtcgc gcagcacctc ggtgccgtcg
     6301 tcgtagccga aggacacgcc gcgcatctgg acgcgcccgc cgccctccgg cagcggctcg
     6361 gcgccctcgg gtgcggtcag gcgtggctcg cggtccagga tctcgaacac gcggttgccg
     6421 gacgcgaccg cgcgctgggc catgcccagc gatacgccca gcatgcgcat cgggccggtg
     6481 agcatgacca cgtagccgta gaaggccacg aagtcgccga cgctgatggt gccgttgatg
     6541 gcctggcggc cgccgaccag caggatcgcc gccagcccca gctggggcag gaagccgatg
     6601 aacgggttgt agaacgcgcg caggcgggtg gagaccatcg actggtcgaa gacccgcgtg
     6661 acggcctggt tgaagcggtg cacctgccgc gtctcctgcg cgaaggcctt gaccacgcgc
     6721 acgccgccca cgttctcctc ggcctcggcc gtcagctcgg cgatacgctg ctggacctcc
     6781 tgcgaggcgg gccggttcag gcgcccgtag cgcgcggccg cccagatgac cagcggcatc
     6841 ggcgccagcg ccacggcggc caggatcggg ttcaccgcga tcatcacgat cgcggcgatc
     6901 acgatggtca gcagcgcctg catcatgaag atcaggccgt agcccaggaa gaagcgcacg
     6961 gactgcaggt ccaccgtcga gcgcgacatc agctggccgg tctgctggcc gtcgaagaac
     7021 cccagctcca gcgactgcag atggcggtac atgcggttgc gcaggtcgaa ctccacgccc
     7081 agcgagacgc gcccggccac cagccggcgg acgacgctga aggccagccg cagcacgccc
     7141 gcggccacca gcacccagcc cagcagctcc agcgagtcgc cgccctggcg gatgtcgtcg
     7201 atggtgcggc cgatcaggaa cggcagcagc acgccggtgc ccatggcggc ggccgccagc
     7261 agcagcgacg cgatcacgcc ggcgcggtag ggacgtagga atcccagcag tctccgaaag
     7321 accttcacgc cctcacttta ctttctgaag catcattgtc cgccgatgct aggcattcaa
     7381 gtgcgcttga agtcaatccc ggtccagcag ggcgcccacg ctgtcgcgcc ggaagtcgaa
     7441 gatctgcttc aggtcgcgcc gcaccagcgg catcgccagc tcgcccagcg gccacagcgg
     7501 cagggcgtag cgcacggtgt cgcgcatcag ggtcgacccg cccctcgcct cgaacgtgtg
     7561 ggtgtggtgc cacagccggt aggggcccac cagctgctgg tccacgaacc gcaccccggg
     7621 ctcccactcg tcgatccgcg tcagccagtc caccggcagc ccatgcagct tcaggcggta
     7681 ctggatgatc gcgccggccc gcatctcgat cggctcctgg ttggtcacgg cgaaccccag
     7741 cagcggcggc gtgatgcgct ccaggttgcg ggcctcggcg aagaacggga acacccggtc
     7801 gaccgggtgg ggcagaagct gctcgcgctg gaggacgtgg accttcacac ggggactacg
     7861 gcccggcgac ggcttccgga tcgccgtccg gcgagtcgtc cagccccttc cagaccagcc
     7921 agccgagcac gatcgaggcc accatgtggg tgatgcccac cagggcgacc acccaccagg
     7981 tctcgacgac gatgaccacc aggccgaggc cgatcatccc ggcgaagaag gcgatgatgg
     8041 tgagcgggac cacgggaccg tgaggctatc cgccccgggg gcgctcccgg ccggtcaagt
     8101 cgccgtcccg cgatgccgat ggggttgggt agcccatggc cgcccggcaa taccgagacc
     8161 tcggtctcgc agagcgtccc gctcgggtgg ccctcgtcgc gggcatcctg ttcgccctga
     8221 tcctggcgtg gcgcttcgcg gcggacgacc cggccagcgc gatcggcgcg atctacgtgg
     8281 tccccgtggc attcctcgcc gtgcacctcg gtgtgcgcgg gggcatgacg ggggcggccg
     8341 tggccatcgg gctctccgcc ctgtggtccg aggtccaggg ggtcgc
//
LOCUS       DASUPW010000499         3057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_669465, whole genome shotgun sequence.
ACCESSION   DASUPW010000499 DASUPW010000000
VERSION     DASUPW010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669465"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VFY44_11210"
     CDS             <1..726
                     /locus_tag="VFY44_11210"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911054.1"
                     /translation="LIGAFFLMTMIVGVGARAILGAGAEEASAGGNLATPLLAEELGG
                     GAGTTGGDLFLAFISAVAFATILAVVAGLVIAASGAVAHDLWTGVKNKGVENEQEEVR
                     VARFTAVGLGVVAILVSIAAGEKFNVSILIGLAFAVAASANLPPLLCTLFWPRFNTMG
                     AVAGVVGGLTVAVVLIILSPQVWPGPDGEGSPVSLTNPAIISIPAGFLFCFLGTVLSR
                     EKSDPSSYHELYVRSEVGLGAAE"
     gene            775..2268
                     /locus_tag="VFY44_11215"
     CDS             775..2268
                     /locus_tag="VFY44_11215"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5911055.1"
                     /translation="MNVAIVGAGFSGIAMGMALKRDGVEDFTIFERGPDLGGVWRANH
                     YPGSACDVPSYLYSYKGEQRRDWTRPCSPQQEILGYLHDVADKNGVAPRIRTDTEIAS
                     AEFDPDAGRWSLRTTTGEEHHADALVVACGQLSRPSWPSIPGRDDFEGHSFHSAEWDH
                     DYDLAGKRVAVVGTGASAVQFVPPVAEDVAQLDVYQRSPPWMLPRSNQEYPRWGRWLL
                     KHVPGLQALRRRYLLAVMESTILGLTKVPPLRWLLGAWAQAFMRMQVRDPELRRKVWP
                     DYPIGCKRVLFSSHYLPALQRPNVEVVTDSIERITPAGVVAGGRERAVDCIVWGTGFR
                     ASDFVVPMRVTGRKGRELQDVWSGGAEAHLGITVAGFPSMYLLYGPNTNLGVGSIVEM
                     IEAQVRYVSGALAATRAEGAPLDLRPEVQRWSGDLVQDRLRNSIWTACDNWYRQDGEG
                     RVVNNWPGFMAEYRRLTREFDRSEYAVASEEWDQGTAATTTRATPTA"
     gene            2217..>3057
                     /locus_tag="VFY44_11220"
     CDS             2217..>3057
                     /locus_tag="VFY44_11220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX5911056.1"
                     /translation="MGPGHGGHDHAGHSHGVDATSNPRWLWIALGLNFAFMLVEVAVG
                     LIANSVALLSDAAHMLTDTASIGLALAAIRLAARPPSGSFTFGLKRSEILSAQLNGAS
                     LLVLAAFLAYEAVGRLSDPPAVEGGLVIVVGVVGALVNLAAALSLARANRTNLNVEGA
                     YLHNLADLWSSVAAIAAGVIIVTTGFDQADGIAALTVCALMLWGGFGLIRDSGRILLE
                     GAPKGMDPDRIGTALASQPGIVEVHDLHVWEVTSGFPALSAHVLVAAGDDCHARRREL
                     QRVL"
BASE COUNT          402 a         1076 c         1126 g          453 t
ORIGIN      
        1 ctgatcgggg cgttcttcct gatgacgatg atcgtgggcg tgggcgcgcg ggccatcctg
       61 ggcgcgggtg ccgaggaggc cagcgccggt ggcaacctgg ccacgccgct gctggccgag
      121 gagctgggcg gcggtgccgg caccaccggc ggcgacctgt tcctggcctt catcagcgcc
      181 gtcgccttcg cgacgatcct ggcggtggtg gccggcctgg tcatcgccgc gtcgggcgcg
      241 gtggcccacg acctctggac cggcgtcaag aacaagggcg tcgagaacga gcaggaggag
      301 gtccgggtgg cgcggttcac cgcggtcggc ctgggcgtgg tggcgatcct ggtctcgatc
      361 gccgcgggcg agaagttcaa cgtctcgatc ctgatcggcc tggcgttcgc cgtcgccgcc
      421 agcgcgaacc tgccgccgct gctgtgcacg ctgttctggc cccggttcaa caccatgggc
      481 gccgtcgccg gcgtggtcgg cggtctgacg gtggccgtgg tcctgatcat cctgagcccg
      541 caggtgtggc cgggcccgga cggggagggg tcgccggtca gcctgaccaa cccggccatc
      601 atctcgatcc cggccggctt cctgttctgc ttcctgggca cggtgctcag ccgcgagaag
      661 agcgacccca gcagctacca cgagctgtac gtgcgctcgg aggtcggatt gggcgcagcc
      721 gaataaagct ggccagccag ccagttccgc gggagatctg ccaccatcgc cgccgtgaac
      781 gtggcgatcg tcggcgcggg gttcagcggg atcgccatgg gcatggcgct caaacgcgac
      841 ggcgtcgagg acttcacgat cttcgagcgc ggcccggacc tgggcggcgt gtggagggcc
      901 aaccactacc ccggctcggc ctgcgacgtg ccgtcctacc tctactcgta caagggcgag
      961 cagcggcgcg actggacccg gccgtgctca ccgcagcagg agatcctggg ctacctgcac
     1021 gacgtcgcgg acaagaacgg cgtcgcaccc aggatccgga ccgacaccga gatcgcgtcc
     1081 gccgagttcg accccgacgc ggggcgttgg agcctgcgca ccacgaccgg tgaggagcac
     1141 cacgccgacg cgctggtggt ggcctgcggc cagctcagcc ggcccagctg gccctcgatc
     1201 cccggtcgcg acgacttcga gggccactcg ttccactcgg ccgagtggga ccacgactac
     1261 gacctggccg gcaagcgcgt ggcggtggtg ggcaccggcg ccagcgccgt gcagttcgtg
     1321 ccgcccgtgg ccgaggacgt cgcgcagctg gacgtctatc agcgctcacc gccgtggatg
     1381 ctgccgcgca gcaaccagga gtacccgcgc tgggggcgct ggctgctgaa gcacgtgccc
     1441 ggcctccagg cgctgcggcg gcgctacctg ctcgcggtca tggagtcgac gatcctgggc
     1501 ctgaccaagg tgcccccgct gcgctggctg ctgggcgcct gggcccaggc cttcatgcgc
     1561 atgcaggtgc gcgacccgga gctccggcgc aaggtctggc ccgactaccc gatcggctgc
     1621 aagcgcgtcc tgttcagcag ccactacctg cccgccctgc agcgcccgaa cgtcgaggtc
     1681 gtgaccgact cgatcgagcg gatcaccccc gccggcgtgg tggccggggg acgcgagcgg
     1741 gcggtggact gcatcgtctg gggcacgggc ttccgggcca gcgacttcgt ggtcccgatg
     1801 cgggtcaccg gccgcaaggg ccgtgagctg caggacgtct ggtccggcgg ggcggaggcc
     1861 cacctgggga tcaccgtcgc gggcttcccc tcgatgtacc tgctctacgg gcccaacacc
     1921 aacctcggcg tggggtcgat cgtcgagatg atcgaggcgc aggtgcgcta cgtgtccggc
     1981 gcgctggcgg ccacgcgcgc cgagggggcg ccgctggacc tgcgccccga ggtgcagcgc
     2041 tggtcgggcg acctggtcca ggaccggttg cgcaactcga tctggacggc ctgcgacaac
     2101 tggtaccgcc aggacggcga gggccgggtg gtcaacaact ggcccggctt catggccgag
     2161 taccgccggc tcacgcgcga gttcgatcgg tcggagtacg ccgtagcatc ggaagaatgg
     2221 gaccagggca cggcggccac gaccacgcgg gccactccca cggcgtagac gccacctcca
     2281 acccgcgctg gctgtggatc gccctcggcc tgaacttcgc gttcatgctc gtggaggtgg
     2341 cggtcggcct gatcgccaac tcggtcgcgc tgctctcgga cgcggcgcac atgctcaccg
     2401 acacggcctc gatcgggctg gcgctggccg cgatccggct ggccgcgcgg ccgccctcgg
     2461 gcagcttcac cttcggcctc aagcgctcgg agatcctgag cgcccagctc aacggcgcct
     2521 cgctgctggt gctggcggcg ttcctggcct acgaggcggt ggggcgcctg agcgacccgc
     2581 ccgcggtcga gggcggcctg gtgatcgtgg tcggggtggt cggcgcgctg gtgaacctgg
     2641 ccgcggcgct gtcgctggcc cgggccaacc gcaccaacct gaacgtggag ggggcgtacc
     2701 tgcacaacct ggccgacctg tggtcgtcgg tggccgccat cgccgcgggc gtgatcatcg
     2761 tgaccacggg cttcgaccag gccgacggca tcgcggcgct gaccgtgtgc gcgctgatgc
     2821 tgtggggcgg cttcgggctg atccgcgact cgggccggat cctcctcgag ggcgcgccca
     2881 aggggatgga ccccgaccgc atcggcacgg cgctggcctc ccagcccggc atcgtggagg
     2941 tccacgacct gcacgtgtgg gaggtcacct cgggcttccc ggccctgtcg gcccacgtgc
     3001 tcgtggccgc gggcgacgac tgccacgccc gccggcgcga gctccagcgc gtgctgg
//
LOCUS       DASUPW010000500         4498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1501306, whole genome shotgun sequence.
ACCESSION   DASUPW010000500 DASUPW010000000
VERSION     DASUPW010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501306"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VFY44_11225"
     CDS             complement(<1..639)
                     /locus_tag="VFY44_11225"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5911057.1"
                     /translation="MHRAWIVAAVGFVVLLASAAIRATFGILMEPLMDEFGWSHGAIS
                     TAAGVNLVVFGLSAPFAATLTERFGLRRTVTAALLLIMVSAAAITQVTELWQLYIVWG
                     LVAGAATGAVAPVLAAAIAGRWFTERRGLVVGSLTAANSTGQLIFLPLMAHLTVMTDW
                     RWVMAVVGAAAVVALVAVALALRDRPADVGLEPYGGLGPETASGTRLSTLGVL"
     gene            710..1663
                     /locus_tag="VFY44_11230"
     CDS             710..1663
                     /locus_tag="VFY44_11230"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5911058.1"
                     /translation="MRKIAGLLLDGSIVFDIGTVLTVFLPRPGTPRPQLPFELTLCSE
                     GGRPVRTGQDVEVSVNAGLEALEDADLIIVPGCAPAMVPGQDAPVEALRRAHERGARV
                     MSICTGAFVLAAAGILDGRRATTHWGYADLMRERHPDVDLDPDVLYIDDGDVLTSAGL
                     SAGMDLCLHVVRTELGAEAANAVARWNVVPPHRDGGQAQFILGPVPEERDGGLASTRA
                     WALERLGQRLTVEDMARHAHMSDRTFSRRFRAETGHSPKRWLNAQRTEHARRLLETTD
                     LPVEQVARESGFATAAALRIHFERTTATQPTAYRAAFRTAA"
     gene            complement(1815..2732)
                     /locus_tag="VFY44_11235"
     CDS             complement(1815..2732)
                     /locus_tag="VFY44_11235"
                     /inference="COORDINATES: protein motif:HMM:NF006159.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5911059.1"
                     /translation="MSSRLEGRVALVAGATRGAGRGIAVALGEAGATVYCTGRSTRAR
                     RSEYDRPETIEETAELVDEAGGTGIAAACDHLDPAAVRELVGRIDSEQGRLDVLVNDI
                     WGAEKLFSFDTPLWEHDLDDGLRLLRLAVETHLVTSHAALPLLIRRPGGLVVEMTDGT
                     RDYNDSRLRVNPFYDLAKASVLRLAFAQAEQLEPHGCTAVALTPGWLRSEMMLEAFGV
                     TEANWDEPGAAPPHFAAISESPRFVGRAVAALAADADVAKRSGGSFSSGELAREYGFT
                     DLDGSQPDCWRYMVEVQDPGLPADPTGYR"
     gene            complement(2716..3639)
                     /locus_tag="VFY44_11240"
     CDS             complement(2716..3639)
                     /locus_tag="VFY44_11240"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX5911060.1"
                     /translation="MRISGIIFCLASAAAFGALGVFGKLAYDEGSTAATLLSTRFVFA
                     AGVLWLVLAATGGMRRLRMLGRRDVLLALGLGAVGYGAQAACYFAALERMDASLLALV
                     LYTFPAMVAVAAIALGRDRASLRTGVALVLAGTGLALVLTGAAAGSLRPAATILGLGA
                     AAVYTVYILTSEGIATRVDPLALATLVCTGAAVTLTGAALATGGLDPAAVTAPGWLWL
                     ASLAVVSTVGAIALFFAGLRRVGPTAAAILSTLEPVVTVALASAVFGETLGPVQLAGG
                     ALVLLAAVAVRVPDKMMERTPIPEVAREQPA"
     gene            3714..>4498
                     /locus_tag="VFY44_11245"
     CDS             3714..>4498
                     /locus_tag="VFY44_11245"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5911061.1"
                     /translation="MLDLRRLRLLRELHERGTVAAVAQALQFTPSAVSQQLAVLEREA
                     GVALLEPAGRGVRLTDPAIVLVRHAEALLDRADLAEAELAAAAGQVSGRARIASFQSV
                     ALKVAIPAIRALAADHPGLRCELRESEPEVALPALQLGDVDLVLGDEWQDQPRPRPAG
                     VHRADLHRDPVRLILPAGHPATRGRTRTARLADLAGEAWTTGYPGAGWEQVVQRACRQ
                     MGGFEPDIRHRTNDSVISLALVASGQAVMLLPELVAHLERHGYV"
BASE COUNT          625 a         1708 c         1653 g          512 t
ORIGIN      
        1 cagcacgccg agagtgctca ggcgcgtgcc cgaggccgtc tcggggccca gcccgccgta
       61 gggctcgagg cccacgtcag cgggccggtc gcgcagggcc aacgcgaccg ccaccagcgc
      121 caccaccgcg gcggcgccca ccacggccat gacccagcgc cagtcggtca tcacggtcag
      181 gtgggccatc agcggcagga agatcagctg gccggtggag ttggccgcgg tcagcgagcc
      241 caccaccagg ccgcgccgct cggtgaacca gcggccggcg atcgccgccg ccaggaccgg
      301 ggccacggcg ccggtggcgg cgccggccac caggccccag acgatgtaga gctgccacag
      361 ctcggtgacc tgggtgatgg ccgcggcgct gaccatgatc agcagcagcg ccgcggtcac
      421 ggtgcggcgg agcccgaagc gctcggtcag cgtggcggcg aacggcgcgc tcaggccgaa
      481 gaccacgagg ttcacgcccg ccgcggtgga gatggcgccg tggctccatc cgaactcgtc
      541 catgagcggc tccatgagga tgccgaaggt ggcccggatg gcggccgagg ccagcaggac
      601 gacgaagccg acggcggcga cgatccacgc gcggtgcatc gccccaggtt cgcccagccg
      661 gccggttcgc accagtggca gaaaagacaa tgttcgctat gtttcggcca tgcgcaagat
      721 cgccggcctg ctgctcgacg gctcgatcgt gttcgacatc gggaccgtgc tcaccgtgtt
      781 cctgccccgc cccgggacgc cccggccgca gctgccgttc gagctgacgc tgtgctcgga
      841 gggcggccgc cccgtccgca cggggcagga cgtcgaggtc tcggtgaacg cgggcctgga
      901 ggcgctggag gacgccgacc tgatcatcgt gcccggctgc gcgccggcca tggtgcccgg
      961 ccaggacgcc ccggtcgagg cgcttcgccg cgcgcacgag cgcggcgcgc gggtcatgtc
     1021 catctgcacc ggcgcgttcg tgctggccgc cgccgggatc ctggacgggc gccgggccac
     1081 gacccactgg ggctacgccg acctgatgcg cgagcgccac ccggacgtcg acctggaccc
     1141 cgacgtgctg tacatcgacg acggcgacgt gctgacctcg gccgggctgt ccgccggcat
     1201 ggacctgtgc ctgcacgtgg tgcgcaccga gctcggcgcc gaggcggcga acgcggtcgc
     1261 gcgctggaac gtggtgccgc cccaccgcga cggaggccag gcccagttca tcctgggccc
     1321 ggtgcccgag gagcgcgacg gcggcctggc ctccacgcgc gcgtgggcgc tcgagcgcct
     1381 ggggcagcgg ctgaccgtgg aggacatggc ccgccacgcc cacatgagcg accgcacctt
     1441 ctcgcgccgc ttccgcgccg agaccggcca ctcgccgaag cggtggctga acgcccagcg
     1501 caccgagcac gcccgccgcc tgctggagac caccgacctg cccgtcgagc aggtggcccg
     1561 tgaatcgggc ttcgccacgg ccgcggcgct gcggatccac ttcgagcgca ccacggccac
     1621 gcagccgacg gcgtaccggg cggcgttccg gacggccgcc tagcggttcg tcactctgac
     1681 gctgtcccga gcgacggttc acacgtttag ggggccatag cgccccaaac gcatgaacca
     1741 cggccggagg acccgggccg ccacgtcccg gccgctcgct caactcgtgc ggccggcagc
     1801 gaacgcgagc gcgcctagcg gtaccccgtc gggtccgccg gcagccccgg gtcctggacc
     1861 tcgaccatgt agcgccagca gtcgggctgc gagccgtcga ggtcggtgaa gccgtactcg
     1921 cgcgccagct cgccggagga gaacgagccg ccgctgcgct tggcgacgtc ggcgtcggcc
     1981 gccagcgccg ccacggcgcg gcccacgaac cgggggctct cggagatggc ggcgaagtgc
     2041 gggggcgccg cgccgggctc gtcccagttc gcctcggtca cgccgaacgc ctcgagcatc
     2101 atctccgagc gcagccatcc cggcgtcagc gccaccgccg tgcagccgtg cggctccagc
     2161 tgctcggcct gggcgaaggc cagccgcagg accgaggcct tggccaggtc gtagaagggg
     2221 ttcacgcgca ggcgcgagtc gttgtagtcg cgcgtgccgt cggtcatctc cacgaccagg
     2281 ccgcccggcc ggcggatcag caggggcagc gcggcgtggc tggtgaccag gtgggtctcc
     2341 accgccaggc gcagcagccg gagcccgtcg tccaggtcgt gctcccacag cggcgtgtcg
     2401 aagctgaaca gcttctcggc gccccagatg tcgttgacca gcacgtccag ccggccctgc
     2461 tcggagtcga tgcggccgac cagctcgcgc acggccgccg ggtccaggtg gtcacacgca
     2521 gcggcgatgc cggtcccgcc cgcctcgtcc accagctcgg cggtctcctc gatcgtctcc
     2581 gggcggtcgt actccgagcg ccgcgcacgc gtcgagcgcc ccgtgcagta gaccgtcgcg
     2641 cccgcctcgc ccagcgcgac ggcgatgccg cgcccggcgc cacgcgtcgc gccggccacc
     2701 agcgcgaccc tgccctcaag ccggctgctc acgtgccacc tccgggatcg gggtccgctc
     2761 catcatcttg tcgggtaccc gcacggccac ggcggccaac agcacgagcg cgccgccggc
     2821 cagctgcacc gggcccaggg tctcgccgaa gaccgccgag gccagggcca cggtgaccac
     2881 cggctcgagc gtggacagga tggccgcggc cgtggggccg acgcggcgca ggccggcgaa
     2941 gaacagcgcg atggcgccca cggtcgagac caccgccagc gaggccagcc acagccagcc
     3001 gggcgccgtg accgcggccg ggtccaggcc gcccgtggcc agcgccgcac cggtcaacgt
     3061 gacggcggcg ccggtgcaca cgagcgtggc cagggccaga gggtcgacgc gcgtggcgat
     3121 gccctccgag gtcaggatgt agacggtgta gacggcggcc gcgcccaggc ccaggatggt
     3181 ggccgccggc cgcagcgagc ccgccgcggc gccggtgagc accagcgcca ggccggtccc
     3241 ggccagcacc agcgcgacgc cggtgcgcag gctggcccgg tcacggccca gcgcgatcgc
     3301 ggccacggcc accatggcgg ggaaggtgta gagcaccagc gccagcagcg aggcgtccat
     3361 ccgctcgagg gcggcgaagt aacaggcggc ctgggcgccg tagccgaccg cgcccagccc
     3421 cagggccagg agcacgtccc ggcggcccag catccgcagc ctgcgcatgc cgccggtggc
     3481 cgccagcacc agccacagga cccccgcggc gaacacgaag cgggtggaca ggagcgtggc
     3541 cgcggtcgag ccctcgtcgt aggcgagctt gccgaacacc cccagggcgc cgaaggcggc
     3601 cgcggaggcc aggcagaaga tgatcccgga gatgcgcacg cgggcatcct gcctgagccc
     3661 ccgttcagaa acgactctgg ttgttgcgca ttatcggtta gcgttgctta acgatgctcg
     3721 acctccggcg actccggctg ttgcgcgagc tgcacgagcg gggaaccgtg gcggcggtcg
     3781 cgcaggcgtt gcagttcacc ccgtcggcgg tctcccagca gctggccgtg ctggagcgcg
     3841 aggcgggcgt cgcgctgctc gagcccgcgg gccgcggcgt ccggctgacc gatcccgcca
     3901 tcgtgctggt ccgtcacgcc gaggcgctgc tggaccgcgc cgacctggcc gaggccgagc
     3961 tggcggccgc ggccgggcag gtcagcggaa gggctcggat cgcgtcgttc cagtcggtgg
     4021 cgctgaaggt ggcgatcccg gccatccgcg cgctggccgc cgaccacccg gggctgcgct
     4081 gcgagctgcg tgagtccgag cccgaggtgg cgctgcccgc gctgcagctg ggcgacgtgg
     4141 acctggtcct gggcgacgag tggcaggacc agccccgccc ccggcccgcg ggcgtccacc
     4201 gcgccgacct gcaccgcgac cccgtgcgcc tgatcctccc cgcgggccat cccgccaccc
     4261 gcgggcgcac gcggaccgcg cgcctggccg acctggcggg cgaggcatgg accaccggct
     4321 accccggcgc cggctgggaa caggtggtcc agcgcgcctg ccgccagatg ggcggcttcg
     4381 agcccgacat ccgccaccgc accaacgaca gcgtgatcag cctggcgctg gtggcctccg
     4441 gccaggcggt gatgctcctg ccggagctgg tggcgcacct ggagcgccac ggctacgt
//
LOCUS       DASUPW010000501         3892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_809104, whole genome shotgun sequence.
ACCESSION   DASUPW010000501 DASUPW010000000
VERSION     DASUPW010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809104"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VFY44_11250"
     CDS             complement(<1..588)
                     /locus_tag="VFY44_11250"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX5911062.1"
                     /translation="MLIGAHVSTAGGLVNAWERGRDMGCDAIQVFNQSPRQWRPTRWK
                     DDDIAEFRERMADGPIKSIVIHAVYLINCASKDKEIRSKSIASLVHSLQMGDAVGADG
                     VVLHPGSTVGEPHGESMERIGKALTQALAESDSCPLLLEDTAGAGNTIGRTFEELGEL
                     VSLAGGGKRVGICLDSCHLLASGYDVRTADAVAATM"
     gene            613..1878
                     /gene="gltX"
                     /locus_tag="VFY44_11255"
     CDS             613..1878
                     /gene="gltX"
                     /locus_tag="VFY44_11255"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX5911063.1"
                     /translation="MRVRFAPSPTGQLHIGGARTALYNWLLARKHGGTLVLRIEDTDR
                     ERSTPENVEQILDALRWLELDWDDGPISQVERAGRHREQIDRLLADGAAYEDDGAVRL
                     RVPDEGETVVSDVIRGDVTFPHSAIDDFVIARSDGSALYNLAVAVDDLDMGIDHVVRG
                     EDHLSNTPRQVMILKALGAQPPVYAHLPLLHGPDGKKLSKRHGAASVQELRDQGYLPE
                     AVRNYIAMLGWGLDDETTFISTDDLIQAFSLERVSRSPAVFDEQKLRWMNGRYLRELT
                     TQDLAARLKPLLGRDDDLEPAVAVAQEKMQTLSDFWPLAAFLLEPQETDPKAWDKVMK
                     EGSAEALAAARDALAAAEPFDVETTEAALRGVVDRLEVKPKQVFQPVRVAISGSTISP
                     GIFESVVALGRDETLARVDAALERLRAHA"
     gene            1953..3026
                     /locus_tag="VFY44_11260"
     CDS             1953..3026
                     /locus_tag="VFY44_11260"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX5911064.1"
                     /translation="MEAVIERTPASVASQGDRPSEGHGRRLTAAFEALENFPVLSESR
                     NRVLRLVRERPDAVGDVVQVVESDVALVTTVLRVANRVAPKGRGKIASVPQAVEVLTP
                     EGVERLAQAANVFDFFERIPGWDAQPERFRLHAVATQAAADRIGRELDYDDRDQLLTS
                     ALLHDIGKLVLVHAYPAYPEQIHGKARTPEERVHAERRELGVDHGLVGGVLARRWGLP
                     ASLATAIERHHSDDATGEAAIVRLADMLAHYGHGAAVDPKQLLNAARALELGPEQLRN
                     LMYEIPYSSNGKKRHVDPCPLSSRELDVLKKLAEGKVYKQIAVELELSTSTVRTHLHN
                     TYAKLGTGDRAQAVLLATDRGWL"
     gene            complement(2987..3328)
                     /locus_tag="VFY44_11265"
     CDS             complement(2987..3328)
                     /locus_tag="VFY44_11265"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX5911065.1"
                     /translation="MALIFAFSAMPSDVEERAWWDVALRKGIHFGEYAVLCALWFRAL
                     RSRWTRDRALAAAVGICVLYAVTDEFHQSFVDGRIGTPRDVLIDTVGVLVAAALLRLY
                     SQPRSVASSTA"
     gene            3403..3804
                     /locus_tag="VFY44_11270"
     CDS             3403..3804
                     /locus_tag="VFY44_11270"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX5911066.1"
                     /translation="MLAITSKAPYAVRALTELARRGSSDPVPIGEIAKAREIPVQFLE
                     GLFATLRRSGVLHSQRGVKGGYLFARDPSEITVLEVVELLEGDLGQDANQAGEIWVQA
                     VGAVKAVLGGTTIADVAEREERDSTAPMYYI"
BASE COUNT          615 a         1440 c         1330 g          507 t
ORIGIN      
        1 catggtggcc gccacggcgt cggcggtgcg cacgtcgtag ccgctggcca gcaggtggca
       61 ggagtccagg cagatgccca cccgcttgcc gcccccggcc agcgagacca gctcgcccag
      121 ctcctcgaac gtgcggccga tcgtgttccc ggcgccggcg gtgtcctcca gcagcagcgg
      181 gcaggagtcc gactcggcca gcgcttgggt gagcgccttg ccgatccgct ccatcgactc
      241 cccgtgcggc tcgccgacgg tggagcccgg gtgcaggacc acgccgtccg cgcccacggc
      301 atcgcccatc tgaagcgagt gcaccagcga ggcgatcgac ttcgaccgga tctccttgtc
      361 cttggaggcg cagttgatca ggtacacggc gtggatcacg atcgacttga tcgggccgtc
      421 ggccatccgc tcgcggaact cggcgatgtc gtcgtccttc cagcgggtgg gccgccactg
      481 gcgcggcgac tggttgaaca cctggatggc gtcgcagccc atgtcgcggc cgcgctccca
      541 ggcgttgacc agcccacccg ccgtggacac gtgggcgccg atcagcacgg ctgatgaata
      601 aggtcgaaac gcatgagggt ccgcttcgcg ccatcaccga caggacagct gcacatcggc
      661 ggcgcgcgca ccgcactcta caactggctg ctggcgcgaa agcacggcgg cacgctggtg
      721 ctgcgcatcg aggacaccga ccgcgagcgc tcgacgccgg agaacgtgga gcagatcctg
      781 gacgccctgc gctggctgga gctggactgg gacgacggtc cgatcagcca ggtcgagcgc
      841 gccggccgcc accgtgagca gatcgaccgc ctgctggccg acggcgccgc ctacgaggac
      901 gacggcgcgg tccgcctgcg cgtgcccgac gagggggaga ccgtggtctc cgacgtcatc
      961 cgcggcgacg tgacgttccc gcactcggcc atcgacgact tcgtgatcgc ccgctcggac
     1021 ggcagcgcgc tctacaacct ggccgtggcc gtcgacgacc tggacatggg catcgaccac
     1081 gtggtccggg gcgaggacca cctgtcgaac accccgcgcc aggtcatgat cctcaaggcc
     1141 ctgggcgccc agccgcccgt gtacgcccac ctgccgctgc tccacggccc cgacggcaag
     1201 aagctgtcca agcgccacgg cgccgcctcg gtgcaggagc tgcgcgacca gggctacctg
     1261 ccggaggcgg tgcgcaacta catcgccatg ctgggctggg ggcttgacga cgagaccacg
     1321 ttcatctcca ccgacgacct gatccaggcc ttctcgctgg agcgcgtgtc gcgctcgccc
     1381 gcggtgttcg acgagcagaa gctgcgctgg atgaacggcc gctacctgcg cgagctgacc
     1441 acgcaggacc tggcggcacg gctgaagccc ctgctgggcc gtgacgacga cctggagccc
     1501 gccgtggccg tggcccagga gaagatgcag acgctgtcgg acttctggcc cctggccgcg
     1561 ttcctgctgg agccccagga gaccgacccc aaggcctggg acaaggtgat gaaggagggc
     1621 tccgcggagg cgctggcggc agcccgcgac gccctggccg cggccgagcc gttcgacgtg
     1681 gagaccaccg aggcggcgct gcgcggcgtg gtcgacaggc tcgaggtcaa gcccaagcag
     1741 gtgttccagc ccgtgcgcgt ggccatcagc ggaagcacca tctcaccggg catcttcgag
     1801 tccgtcgtgg ccctgggccg cgacgagacg ctggcccgcg tggacgccgc gctcgagcgc
     1861 ctgcgcgcgc acgcctgaca ccaacccctc aatagttccc gggggctgcc gatagaggtt
     1921 gagactcctg gcttcggcca cctgacccca tcgtggaagc cgtcatcgaa cgcacacccg
     1981 ccagcgtcgc ctcccagggc gatcgcccca gcgaaggcca cggccggcgc ctgaccgcgg
     2041 cgttcgaggc gctggagaac ttccccgtcc tgtcggagtc ccgcaaccgc gtcctccgcc
     2101 tggtccgcga gcgtcccgac gccgtcggcg acgtggtcca ggtggtcgag tccgacgtgg
     2161 cgctggtgac caccgtgctg cgggtggcca accgcgtcgc gcccaagggc cgcggcaaga
     2221 tcgccagcgt gccccaggcg gtggaggtgc tgacgccaga gggcgtcgag cgcctggccc
     2281 aggccgccaa cgtcttcgac ttcttcgagc gcatccccgg ctgggacgcc cagcccgagc
     2341 gcttccgcct ccacgccgtg gccacccagg ccgcggccga ccggatcggc cgcgagctgg
     2401 actacgacga ccgcgaccag ctgctgacct cggcgctgct gcacgacatc ggcaagctcg
     2461 tgctggtgca cgcctacccg gcctacccgg agcagatcca cggcaaggcg cgcacccccg
     2521 aggagcgcgt ccacgccgag cgccgcgagc tcggcgtcga ccacggcctg gtcgggggcg
     2581 tgctggcccg ccgctggggc ctgccggcat cgctggccac ggccatcgag cgccaccact
     2641 ccgacgacgc caccggcgag gccgccatcg tgcgcctggc cgacatgctg gcccactacg
     2701 gccacggcgc cgccgtcgat cccaagcagc tgctgaacgc cgcccgcgcc ctggagctcg
     2761 ggcccgagca gctccggaac ctgatgtacg agatccccta ctcgtccaac ggcaagaagc
     2821 gccacgtcga tccgtgcccg ctctccagcc gcgagctgga cgtgctcaag aagctggccg
     2881 agggcaaggt ctacaagcag atcgcggtcg agctggagct gtccaccagc accgtgcgca
     2941 cgcacctgca caacacctac gccaagctcg ggaccggcga ccgcgctcag gccgtgctgc
     3001 tggccaccga ccgcggctgg ctgtagagcc tcagcagcgc cgcggccacc aacacgccca
     3061 ccgtgtcgat cagcacgtcg cgcggtgtgc cgatccgccc gtcgacgaac gactggtgga
     3121 actcgtcggt gaccgcgtag aggacgcaga tgcccaccgc ggcggccagc gcgcggtccc
     3181 gcgtccagcg gctgcgcagc gcgcggaacc acagcgcgca cagcaccgcg tactcgccga
     3241 agtggatgcc cttgcgcaac gcgacgtccc accacgcccg ctcctcgacg tcgctcggca
     3301 tcgccgagaa ggcgaagatc agcgccatca gcgcgaccgg cggaccccaa agggccaata
     3361 ggcggacggg tttcgacatg aacgccccgt acactggcag aggtgctggc gatcacctcc
     3421 aaggccccgt acgcggtgcg ggcactgacc gaactggcgc ggcgcggtag cagcgacccg
     3481 gtgcccatcg gcgagatcgc caaggcgcgc gagatcccgg tgcagttcct ggaggggctc
     3541 ttcgccacgc ttcgccgctc cggcgtcctg cacagccagc gcggcgtcaa gggcggctac
     3601 ctgttcgccc gcgacccgtc ggagatcacc gtgctcgagg tggtcgagct gctggagggc
     3661 gacctgggcc aggacgcgaa ccaggccggc gagatctggg tccaggccgt cggcgccgtc
     3721 aaggccgtcc taggtggcac gaccatcgcc gacgtggccg agcgcgaaga gcgcgactcc
     3781 acggcgccga tgtactacat ctagaagcaa gcgcggccgg cagccgctat ccgctaccgg
     3841 ggcgcggcag ggtccacgcg gtagcgagtg accgtgtcat ggacgtcgtc gt
//
LOCUS       DASUPW010000502         2224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_532721, whole genome shotgun sequence.
ACCESSION   DASUPW010000502 DASUPW010000000
VERSION     DASUPW010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532721"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..874)
                     /gene="coxB"
                     /locus_tag="VFY44_11275"
     CDS             complement(155..874)
                     /gene="coxB"
                     /locus_tag="VFY44_11275"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX5911067.1"
                     /translation="MRRRPMLWTIVFSVVGTIVGIAICLQIDWFPTQASSAAPDIDTL
                     YDVLLIVSVPIFVLVMAVVFYSVVAFKAQPGDLGDGAPIHGNTRLEVIWVTIPFIIVS
                     ALAAYGWVVLDDIEAKEPDALVVNVTGQQFAWRFDYPDNGKVKVNQLVLPKGRQVHFR
                     VNTDDVIHDFWVPQFRLKTDAVPGLTTDIRTTPTRVGTYQVVCAELCGIGHATMRQQV
                     RVVPAATFDRWIARKGGGGQR"
     gene            complement(980..1354)
                     /locus_tag="VFY44_11280"
     CDS             complement(980..1354)
                     /locus_tag="VFY44_11280"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5911068.1"
                     /translation="MLNEAKRPATVQRRAELFREAAAIIDSEYEAKLALDDVARRVAT
                     SRRQLQRAFAEAGETSFRSYLQRVRMEQAARLLQGSASPVNEVASAVGYRQPAQFAKA
                     FRRHHGTPPSSFREAAGASLAA"
     gene            1515..1901
                     /locus_tag="VFY44_11285"
     CDS             1515..1901
                     /locus_tag="VFY44_11285"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5911069.1"
                     /translation="MSTVILTVGPDHTLAQVAAAMTDRRVGAAVVVDPEQPGPGIITE
                     RDILAALGAGQDPAEEKVGAHLSTDLTFAEPTWSLEYAAEAMVRGGFRHLVVIDGGEL
                     VGVLSMRDIVRCWVQEGAVSEMPAAS"
BASE COUNT          331 a          765 c          806 g          322 t
ORIGIN      
        1 agggcgacgg tcgtgaccgc cgtcatgtcc aggaccggat cccagccgta cagcgcgcgt
       61 acgagggcga ccagccccag cgcgaagagg taggccacgg gcagggccca cgccactcgg
      121 taccagccgg gagccctgag cttggcggcc atcgtcatcg ctgtcctcct ccgcccttcc
      181 tggcgatcca tctgtcgaac gtcgccgcgg gcacgaccct gacctgctgg cgcatcgttg
      241 cgtgcccgat gccgcacagc tcggcgcaga ccacctggta ggtgccgacg cgggtgggcg
      301 tggtgcggat gtcggtggtc aggcccggca cggcgtcggt cttgagccgg aactggggca
      361 cccagaagtc gtggatgacg tcatcggtgt tcacgcggaa gtgcacctgg cggcccttcg
      421 gcagcaccag ctggttgacc ttgaccttgc cgttgtcggg gtagtcgaag cgccaggcga
      481 actgctggcc ggtcacgttg accaccagcg cgtcgggctc cttcgcctcg atgtcgtcga
      541 ggacgaccca gccgtaggcg gccagcgcgg agacgatgat gaacgggatc gtgacccaga
      601 tgacctcaag gcgggtgttg ccgtggatgg gggcgccgtc gccgaggtcg ccgggctggg
      661 ccttgaaggc caccacggag tagaagacca ccgccatgac cagcacgaag atggggacgg
      721 acacgatcag caggacgtcg tagagcgtgt cgatgtccgg cgcggccgag gaggcctgcg
      781 tcgggaacca gtcgatctgg aggcagatcg cgatgccgac gatggtgccg accacgctga
      841 agacgatcgt ccacagcatc ggccgccgtc gcaccctaga catccttcac cgcactcctc
      901 cgaaacacgg tgcggaaccc tacacctaga accggggggt tcgcactccc cgcgcgcggg
      961 gtctggggtc agcggcgggt caggcggcca ggctggcgcc cgcggcctcg cggaagctgg
     1021 acggcggcgt gccgtggtgg cggcggaagg ccttggcgaa ctgggccggc tggcggtatc
     1081 cgaccgcgct ggccacctcg ttcacgggcg aggcgctgcc ctggagcagc cgggccgcct
     1141 gttccatgcg cacgcgctgg aggtagctgc ggaagctggt ctcaccggcc tcggcgaacg
     1201 cgcgctggag ctggcggcgc gaggtcgcca ctcggcgggc gacgtcgtcg agcgcgagct
     1261 tggcctcgta ctcggagtcg atgatcgccg cggcttctct gaagagctcg gcacggcgct
     1321 ggacggtggc tgggcgcttg gcttcgttga gcatggtcac ttggtcgtac gcgccagcgg
     1381 cccaaatgga taaccgcctc gcgaaatttg tgcctcccgc caccgcaacg ggcgtccgcc
     1441 gccatatgca aagctataga tagcgccaat cgtcacatga ctttccagga ggacggatgc
     1501 aggttcaggc agggatgagc acggtcattc tcacggtcgg acccgaccac acgctggccc
     1561 aggtggcggc cgcgatgacc gaccggcgcg tgggcgccgc cgtggtggtg gaccccgagc
     1621 agcccggtcc cggaatcatc accgagcgcg acatcctggc cgcgctcgga gccggccagg
     1681 accccgcgga ggagaaggtc ggcgcgcacc tctcgaccga cctcacgttc gccgagccca
     1741 cctggtcgct ggagtacgcc gccgaggcga tggtcagggg cggcttccgc cacctggtcg
     1801 tgatcgacgg cggcgagctg gtcggcgtgc tctccatgcg cgacatcgtc cgctgctggg
     1861 tccaggaggg cgcggtctcg gagatgccgg cggccagcta gggccggcgg cgctgccgtt
     1921 ctgccgcggg cgtccccggc gggtccggcg caacggcgag gctgggtccg gcggcacggt
     1981 tccgtgcgtc ttccgtgacc gttcgcgcca atccgtgacg cccggatcgc tccgcggcgc
     2041 atttctcccg ctcagcggga gcaatccacc gcgcggcgtt cagcgaggtc gcgggagagc
     2101 ggtcccgccc ggaccgcggc aagaccccgc cgccccaccc agccccgccg ttgcgccggc
     2161 cccgccgggg acgcccgcgg cagaacggca gcgccgccgg ctagctggcc gccggcatct
     2221 ccga
//
LOCUS       DASUPW010000503         3222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1779654, whole genome shotgun sequence.
ACCESSION   DASUPW010000503 DASUPW010000000
VERSION     DASUPW010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1779654"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VFY44_11290"
     CDS             complement(<1..311)
                     /locus_tag="VFY44_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911070.1"
                     /translation="MLVAALILVFAVTAVGCGGDDKDSSGSGDNSSQSGDSGSKDSGS
                     SGSADDTPDNVDEAVDKCLEEADKAPDGDAKEAAKKLCNAAKSRDPEKIKDSARDACL
                     DL"
     gene            complement(431..1072)
                     /locus_tag="VFY44_11295"
     CDS             complement(431..1072)
                     /locus_tag="VFY44_11295"
                     /inference="COORDINATES: protein motif:HMM:NF024197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HEX5911071.1"
                     /translation="MDVGTEITKLEGLPEPVWRRIVANPERAPELIALAASKRFADPA
                     AEWVRENSSWRQPARLAKKAYSKHVHLSRIEGLALGLGGMVTSAFNVAGLGWLQARMV
                     FYIAAALGHDPHDPMRPAELLFLWGVYESPEQARESLEGIGESMAVTVVKTQLSKPSQ
                     RTLTNRMLRYVGKRTAKRYGGRMIPLLGSPISAVQNGGSTKDLGRRALKYYGG"
     gene            complement(1095..1496)
                     /locus_tag="VFY44_11300"
     CDS             complement(1095..1496)
                     /locus_tag="VFY44_11300"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX5911072.1"
                     /translation="MDVPQGFEPYPEQGPYLEYVGPIHVREGDGPPVLGVRAEERHAN
                     HRGTVQGGLLSTFADFALGRAIGQDARDDHERATVSLTIDFLKPAKPGDWIESRTSVD
                     RVGGTLAFADCVLTVDEKDVARARAVFVVVG"
     gene            complement(1632..1704)
                     /locus_tag="VFY44_11305"
     tRNA            complement(1632..1704)
                     /locus_tag="VFY44_11305"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1669..1671),aa:Phe,seq:gaa)
     gene            1731..2672
                     /locus_tag="VFY44_11310"
     CDS             1731..2672
                     /locus_tag="VFY44_11310"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX5911073.1"
                     /translation="MDLVRSRLAAQHLCPATAASSALEAARTVCGFQAQDVRSAGLAL
                     RSRVPGLTRAAVRDAGLVRTWTVRGTLHLIAPEDRAWLHALCAPRYLPQMEKALEKRG
                     GLDTARGMLDDLRELVADEPLERAALLERLAERGHAELGDRAVNALLPWVALQGLIVG
                     TADGRWAGSEPPEPVDEDEALATLGRRYLVGHGPAGDRDLAAWSGLPLGRARRALELA
                     GPVDAAAPAEPPACALLAAFDPAMLGWETREPLVAARNARDVLPGGGMLRPVVLAGEH
                     VVGTWRGSPPEFAWFGPEPPPDALAAEVADVERFLAT"
     gene            2701..>3222
                     /locus_tag="VFY44_11315"
     CDS             2701..>3222
                     /locus_tag="VFY44_11315"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX5911074.1"
                     /translation="MGTDASAARAQVLLAAVCFGTSGTAQALGPGGAPTSVGAVRIVV
                     GALLLWLIARALSGPAGETAAPGRLAWLAGVGVAVYQLAFFAAVDQTGVAVGTVVAIG
                     SGPAAAGLLSRVVNGEPLTGRWAAATALACGGIVLLGAGGGSASVDPVGVGLAVVAGL
                     GYASYTVLAKRMID"
BASE COUNT          380 a         1125 c         1246 g          471 t
ORIGIN      
        1 agatcgaggc aggcgtcgcg ggcgctgtcc ttgatcttct cggggtcgcg gctcttggcg
       61 gcgttgcaca gcttcttggc cgcttccttg gcgtcgccgt cgggagcctt gtcggcctcc
      121 tcgaggcact tgtcgacggc ctcgtcgacg ttgtcggggg tgtcgtccgc ggagccgctg
      181 gacccggagt ccttcgagcc cgagtcgccg gactgggacg agttgtcgcc ggagccggac
      241 gagtccttgt cgtcgccgcc gcaaccgacg gcggtgaccg cgaacacgag gatgagggct
      301 gctaccagca tcagggagat gcgcttggcc atgtgggtga gtccttccgc ttgagagtgg
      361 tcaggcgaac gtaatgcggg tggccggcgc acgcagtctc ggatgcgtct cgcccgggtc
      421 tcgcccgccg tcagcccccg tagtacttca gcgcccggcg gcccaggtcc ttcgtcgaac
      481 cgccgttctg taccgcgctg atgggggacc ccagcagcgg gatcatgcgc ccgccgtagc
      541 gcttggccgt gcgcttgccc acgtagcgca gcatccggtt ggtcagcgtc cgctggctgg
      601 gcttggacag ctgggtcttg accacggtca cggccatgct ctccccgatg ccctccaggg
      661 actcgcgcgc ctgctcgggg gactcgtaca cgccccacag gaacagcagc tcggccgggc
      721 gcatcgggtc gtgcgggtcg tggcccagcg cggcggcgat gtagaagacc atccgcgcct
      781 ggagccagcc cagaccggcc acgttgaagg cgctggtgac catgccgccc aggcccagcg
      841 ccagcccctc gatacgggac aggtgcacat gcttggagta ggccttcttg gcgagccggg
      901 ccggctggcg ccaggacgag ttctcgcgga cccactcggc cgccgggtcg gcgaagcgct
      961 tcgaggcggc cagcgcgatc agctccggcg cgcgctcggg gttcgccacg atccggcgcc
     1021 agaccggttc cggaaggccc tcgagcttcg tgatctccgt gccgacgtcc acgaggggga
     1081 gcctagggac cggcctagcc caccaccacg aacaccgccc gggcgcgcgc cacgtccttc
     1141 tcgtccacgg tcaggacgca gtcggcgaac gccagcgtcc cacccacgcg gtccacgctg
     1201 gtgcgggact cgatccagtc gcccggcttc gcgggcttca ggaagtcgat cgtcaggctc
     1261 acggtcgccc gctcgtgatc gtcccgcgcg tcctgcccga tcgcccggcc cagggcgaag
     1321 tcggcgaacg tggacagcag cccaccctgc accgtgccgc ggtggttggc gtgacgttcc
     1381 tcggcacgaa cgccaaggac cggaggcccg tcgccctcgc gcacgtggat gggccccacg
     1441 tactccagat agggaccctg ctcgggatag ggctcgaagc cctgggggac gtccatggcg
     1501 gcgagcctat tgcgagtcgg acgcgactca cgaggtcgct ggcccgcaga aggggcgacg
     1561 cagccgacga cgcgtgctcg tgcacgcgag gaggcgagga gccgcttatg cgatgccagc
     1621 ggcccgtgag atggcgagag ccggacttga accggcgaca ccacgatttt cagtcgtgtg
     1681 ctctaccaac tgagctacct cgccgcgggc ggacatccta gctttacgcg atggacctgg
     1741 ttcgctcgcg cctggcggct cagcacttgt gccctgcgac ggccgcctcg tcggcgctgg
     1801 aggcggcgcg cacggtgtgc gggttccagg cccaggacgt gcgctcggcc gggctggccc
     1861 tgcgctcgcg ggtgccgggg ctgacccgcg ccgccgtgcg tgacgcgggc ctggtgcgca
     1921 cctggacggt ccgcggcacg ctgcacctga tcgccccgga ggaccgggcc tggctgcacg
     1981 cgttgtgcgc accccggtat ctgccgcaga tggagaaggc gctggagaag cgcggcggcc
     2041 tggacacggc gcgaggcatg ctggacgacc tgcgggagct ggtggcggac gagccgctgg
     2101 agcgggcggc gctgctcgag cggctggccg agcgcggcca cgccgagctg ggcgaccgcg
     2161 cggtgaacgc gctgctgccc tgggtcgccc tgcaggggct gatcgtgggc accgcggacg
     2221 ggcgctgggc cggctcggag ccgccggagc ccgtggacga ggacgaggcc ctggccacgc
     2281 tggggcggcg ctacctggtc ggccacgggc cggcgggcga ccgcgacctg gcggcctggt
     2341 cgggcctgcc gcttgggcgg gcgcggcgcg cgctggagct ggccgggccg gtggacgcgg
     2401 cggccccggc ggagccgccg gcgtgcgcgc tgctggcggc cttcgacccg gcgatgctgg
     2461 gctgggagac gcgcgagccg ctggtggcgg cccggaacgc gcgcgacgtg ctgccgggcg
     2521 gcggcatgct caggccggtg gtgctggcgg gggagcacgt ggtgggcacg tggcggggct
     2581 cgcccccgga gttcgcgtgg ttcggcccgg agccgccgcc ggacgcgctg gccgccgagg
     2641 tcgccgatgt ggagcggttc ctggcgacct gacgaagatt gtggaacaat cgaggtcgtc
     2701 atgggaaccg atgcctccgc cgcccgcgcc caggtcctgc tggccgccgt ctgcttcggc
     2761 acctcgggca ccgcccaggc gctgggcccc ggcggcgcgc ccaccagcgt gggcgcggtc
     2821 cggatcgtgg tcggcgcgct gctgctgtgg ctgatcgccc gggcgctgtc cggcccggcg
     2881 ggtgagacgg ccgccccggg ccggctcgcg tggctggccg gcgtcggcgt ggcggtgtac
     2941 cagctggcct tcttcgcggc ggtcgaccag accggggtgg ccgtgggcac ggtcgtggcc
     3001 atcggctcag gccccgcggc cgccgggctg ctgtcgaggg tggtcaacgg cgagcccctg
     3061 accggccgct gggcggcggc caccgcgctg gcctgcggcg gcatcgtcct gctcggcgcc
     3121 ggcggcggtt cagcgtcggt cgaccccgtg ggtgtggggc tggccgtcgt ggcgggcctg
     3181 ggctacgcgt cctacacggt gctggccaag cgcatgatcg ac
//
LOCUS       DASUPW010000504         2363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_117240, whole genome shotgun sequence.
ACCESSION   DASUPW010000504 DASUPW010000000
VERSION     DASUPW010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117240"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1478
                     /locus_tag="VFY44_11320"
     CDS             <1..1478
                     /locus_tag="VFY44_11320"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HEX5911075.1"
                     /translation="IAGDYMSAASFLGIAGLIFLFGFDGFLYSVGFLVAFLTVLFLLA
                     ERMRNSGKYTIADTLAFRMNAKPARAAAALGTLTVAAFYLVAQMVGAGALMEPLTGIG
                     FELSIVLVGIFMLIYVVAGGMLATTWVQIIKAVLLLSGTLVLTLFVLNRVGWNPMELF
                     RDARDASPAKDAYLAPGLNYAKNQPWETLSLGLGLVLGTAGLPHILMRFFTVPDAKAA
                     RVSVLWAVILIGVFYLMTTALGFGARAILGEGGVEAAGTGGNLAAPLLAEELGGGKGT
                     VGGDLFLAVIAAVAFATILAVVAGLVISASGAVAHDVWTNIIRNGEENEKEEVLVARI
                     SALAIGIIAILVGVLAKDQNVSFLVGLAFAVAASANFPALLLALTWRRFNTTGAVVGV
                     VVGVVSSVGLVVMSPGVWPGADGSDGHPFPLANPGLISIPLGFLACYLGTVLSGEERK
                     ATKGFDELYVRSETGLGAEEGTGTSLTGRTTEPKKTGEPVA"
     gene            1558..>2363
                     /locus_tag="VFY44_11325"
     CDS             1558..>2363
                     /locus_tag="VFY44_11325"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5911076.1"
                     /translation="MEAGTAPTGQELEDELAELLDQETFEPPAEFVDSALLTDPAIYE
                     EAAKDPVAWWEKQAEDLHWFEKWDTALDESNPPYYKWFQGGKINAAYNCLDRHVDDGK
                     GDKVAFHWRGEEGEQRDVTYAELLADTKKLANALVDNGVEKGDVVGIFLPMIPEVVVA
                     MLACARIGAIHNVVFGGFSPNSVAERMQVSEAKLLITADGARRKGKTAEVKSEVDDVL
                     GEMPTVKTVIVVKSVDNDAPMEDGRDLWWHDALEAADDEHEAEPLDAEHPL"
BASE COUNT          356 a          797 c          843 g          367 t
ORIGIN      
        1 ccatcgcggg cgactacatg tcggcggcat ccttcctggg catcgccggg ctgatcttcc
       61 tgttcggctt cgacggcttc ctgtactccg tcggcttcct ggtggcgttc ctgaccgtgc
      121 tcttcctgct cgcggagagg atgcgcaact cgggcaagta cacgatcgcc gacacgctgg
      181 ccttccgcat gaacgccaag ccggcccgtg cggcggcggc cctgggcacc ctgaccgtcg
      241 cggccttcta cctggtcgcg cagatggtcg gcgccggtgc gctcatggag cccctgaccg
      301 gcatcggctt cgagctgtcg atcgtgctcg tgggcatctt catgctcatc tacgtggtcg
      361 cgggcggcat gctggccacc acctgggtgc agatcatcaa ggcggtgctg ctgctgtcgg
      421 gcacgctggt gctgacgctg ttcgtgctca accgcgtggg ctggaacccg atggagctgt
      481 tccgtgacgc gcgcgacgcc agccccgcca aggacgccta cctggcgccc ggcctgaact
      541 acgccaagaa ccagccctgg gagaccctgt cgctgggcct cggcctggtg ctgggcaccg
      601 cgggcctgcc gcacatcctg atgcggttct tcaccgtgcc ggacgccaag gcggcccgtg
      661 tgtcggtgct gtgggccgtg atcctgatcg gcgtcttcta cctcatgacc accgcgctgg
      721 gcttcggcgc gcgggcgatc ctgggcgagg gcggcgtcga ggccgccggc acgggcggca
      781 acctggccgc gccgttgctg gccgaggaat tgggcggcgg caagggcacg gtcggaggcg
      841 acctgttcct ggccgtgatc gccgcggtcg cgttcgccac gatcctggcc gtggtggccg
      901 ggctggtgat ctcggcctcg ggggcggtgg cccacgacgt gtggaccaac atcatccgca
      961 acggcgagga gaacgagaag gaggaggtgc tggtggcccg catctccgcg ctcgccatcg
     1021 gcatcatcgc catcctggtc ggtgttctgg cgaaggacca gaacgtctcg ttccttgtgg
     1081 ggttggcgtt cgccgtagcc gcgagcgcca acttcccggc gttgttgctc gcgctcacgt
     1141 ggcgcaggtt caacaccacc ggggcggtgg tcggcgtggt ggtcggcgtg gtcagctcgg
     1201 tcgggctcgt ggtcatgagc cccggcgtct ggccgggagc ggacggcagc gacggccatc
     1261 ccttcccgct ggccaacccc ggcctgatct cgatcccgct gggcttcctg gcctgttacc
     1321 tgggcacggt cctcagcggc gaggagcgca aggccacgaa gggcttcgat gagctctacg
     1381 tgcgctcgga gacgggcctc ggcgccgagg agggcaccgg gaccagcctg accgggcgca
     1441 cgaccgagcc caagaagacg ggcgagccgg tcgcctgacc gtgcacgggg cgggacgcgt
     1501 tggtgtcccg ccccgccgca tggggtagaa cccgcagcga agccaccagg aggagatatg
     1561 gaagccggga ccgcgcctac aggacaggag ctcgaggacg agctcgccga gctgctcgac
     1621 caggagacct tcgagccgcc ggccgagttc gtcgacagcg cgctcctgac cgacccggcc
     1681 atctacgagg aggcggccaa ggaccccgtc gcctggtggg agaagcaggc cgaggacctg
     1741 cattggttcg agaagtggga caccgccctc gacgagtcca acccgcccta ctacaagtgg
     1801 ttccagggcg gcaagatcaa cgccgcctac aactgcctgg accgccacgt cgacgacggc
     1861 aagggcgaca aggtcgcctt ccactggcgc ggcgaggagg gcgagcagcg cgacgtgacc
     1921 tacgccgagc tgctggccga caccaagaag ctggccaacg cgctggtgga caacggcgtc
     1981 gagaagggcg acgtggtcgg gatcttcctg cccatgatcc ccgaggtcgt ggtggccatg
     2041 ctggcctgcg cccgcatcgg cgcgatccac aacgtcgtct tcggcggctt ctccccgaac
     2101 tcggtggccg agcgcatgca ggtctccgag gccaagctgc tgatcaccgc cgacggcgcc
     2161 cggcgcaagg gcaagaccgc cgaggtcaag tccgaggtgg acgacgtcct gggcgagatg
     2221 ccgaccgtca agaccgtgat cgtggtcaag tcggtggaca acgacgcgcc catggaggac
     2281 gggcgcgacc tgtggtggca cgacgcgctc gaggccgccg acgacgagca cgaggccgag
     2341 ccgctggacg ccgagcaccc gct
//
LOCUS       DASUPW010000505         4021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1502586, whole genome shotgun sequence.
ACCESSION   DASUPW010000505 DASUPW010000000
VERSION     DASUPW010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502586"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..617)
                     /locus_tag="VFY44_11330"
     CDS             complement(42..617)
                     /locus_tag="VFY44_11330"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX5911077.1"
                     /translation="MFEMARTPLKAALIAAFTVGVVLFLLDPPSLSGLPGMAMWIGGG
                     IALTVLTAWAFVAMMGDEGPSEQQFDRIVERSEALARLPVPELPPDDFDELVTQAIDD
                     LPAEFRDLLETTPVVVSRHGREYHAYGHYIGDTVARDDHPDRIIIYRDTLERDFGHDP
                     DLLRAQVTRTVRHELAHHLGADELGVREMGL"
     gene            671..916
                     /locus_tag="VFY44_11335"
     CDS             671..916
                     /locus_tag="VFY44_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911078.1"
                     /translation="MHLILIIVAALAALVAMVVLAVGMTASRSRREESDRSSALLGLA
                     RLRSPSGKAFFPDDDSVLELAELLDDVDERRRRDTQR"
     gene            948..1949
                     /locus_tag="VFY44_11340"
     CDS             948..1949
                     /locus_tag="VFY44_11340"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX5911079.1"
                     /translation="MREHLQAEDTRRVRVLAAGGTIAMASDGNGGVTPELDGPALLAS
                     MPGLENTHGIDAETVINVPSAHLTLEDQLHICREARDAARRGIGVVITHGTDTLEETA
                     MLCDVVHDAEAPIVITGAIRPASAPGADGPANLSDAVSVAASEAAAGMGVLVVFGGEI
                     HHARCVRKTDTTSLVAFSSPQTGPLGRVTEGHPIIWSKIPRNPPMDPKNLGKRVLVVP
                     TMAGDDGSLAHAALSTEPDGVIIGTLGAGHLGPPLLDVWAEAAKRMPVVAYCRPERGV
                     VLNSTYGYAGSERDLRNTGIIPAGFLSPQAARMKLLACLSSELSIDEIRWAFRQDDG"
     gene            2014..2229
                     /locus_tag="VFY44_11345"
     CDS             2014..2229
                     /locus_tag="VFY44_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911080.1"
                     /translation="MFTALLTCSDEDCTELAAFAGSLEAADDVLCEGCGCLMQVIAIE
                     GGHEPAEVIDLAAHRTRRAGAPGRLAA"
     gene            complement(2226..3362)
                     /locus_tag="VFY44_11350"
     CDS             complement(2226..3362)
                     /locus_tag="VFY44_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911081.1"
                     /translation="MPKRSHTSIALTTAALALACGPAAAEAASVKLVHYDVEADVTLV
                     HDHAYHFATAWESQDTDVQLVSDLHAKLGRITFRDGYLMSPVAQGEATADSSGVAKGH
                     YANKVFSGSRSCDTTTGPLPSGQGTIDSDLLAPLTGEAFNLRVADQVRVDWQCRGDER
                     VSATAPYEYTDNGPALGSGPLDAQFEIPHEAVGYGKIIQNIKGPGGAFCAGAHLAGTT
                     KCAYDWAGDVTFTKTSETVISDVGEPGQAVAPSGSASGQKTPASENLDELLVPLVPAK
                     AATADAKGSTVSLTASCPTGCAGTAALTVAGAKASAAGKARKPVATMRFRVKAGKPRL
                     VKLRVPKKAARALRKAGKGKLAVTLRPKRGRAVRKTLSVKVGKR"
     gene            complement(3439..>4021)
                     /locus_tag="VFY44_11355"
     CDS             complement(3439..>4021)
                     /locus_tag="VFY44_11355"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5911082.1"
                     /translation="TDNIVIGLIAERITGRPYGALLDDFVFGPLGLNETSFPRSTPLP
                     RPFIHGYVTEPGNKPEDVSTLLSPSGAWASGAIVSTPRNLSTFMRGYVSGRLFGTAAR
                     REQRRFVAGGRSSPPGPGANSAGLALFRYRTRCGTVYGHTGNFPGYVQWAAASSDGQR
                     VVTTSLNIPAPEGALLRRLRAVQEDAVCALLRG"
BASE COUNT          539 a         1444 c         1482 g          556 t
ORIGIN      
        1 tcatcgaact gcatcgttcg atgaacatcg tactgcgaag ctcacaaccc catttctcgg
       61 acgccgagct cgtcggcgcc gaggtggtgc gccagctcgt ggcggaccgt gcgggtgacc
      121 tgggcgcgca gcagatccgg gtcgtggccg aagtcgcgct cgagggtgtc gcggtagatg
      181 atgatccggt ccgggtggtc gtcgcgggcc acggtgtcgc cgatgtagtg gccgtaggcg
      241 tggtactcgc ggccgtggcg cgagaccacc accggggtgg tctccaggag gtcgcggaac
      301 tccgcgggca ggtcgtcgat ggcctgggtg accagctcgt cgaagtcgtc cggcggcagt
      361 tcggggacgg gcagccgggc cagggcctcg ctgcgctcga cgatgcggtc gaactgctgc
      421 tcggaggggc cctcgtcgcc catcatggcc acgaaggccc aggcggtcag cacggtcagg
      481 gcgatgccgc cgccgatcca catggccatg ccgggcaggc ccgagagcga cggggggtcg
      541 agcaggaaca gcacgacgcc caccgtgaag gccgcgatca gcgccgcctt gagcggggtg
      601 cgggccatct cgaacatggt ctgagagtag tggcgcgctg agcgggctcg tccatactgg
      661 aggtcccgcc gtgcacctga tcctgatcat cgtcgccgca ctggccgccc tggtggcgat
      721 ggtcgtgctg gccgtgggca tgaccgccag ccgctcccgc cgcgaggaga gcgaccgcag
      781 ctccgcgctg ctggggctgg cccggctgcg cagtccgtcg ggcaaggcgt tcttccccga
      841 cgacgactcg gtcctggagc tggcggagct gctggacgac gtggacgagc gccgccgccg
      901 ggacacccag cgctagccgc agagcccgct cacctagagt gctcagggtg agagagcacc
      961 tgcaggccga ggacacccgg cgcgtgcgag tgctggccgc cggcggcacc atcgccatgg
     1021 ccagcgacgg caacggcggc gtgacccccg agctggacgg gccggcgctg ctggcgtcga
     1081 tgcccgggct ggagaacacg cacgggatcg acgccgagac ggtgatcaac gtgcccagcg
     1141 cgcacctgac gctggaggac cagctgcaca tctgccggga ggcgcgcgac gccgcccggc
     1201 gcggcatcgg cgtggtcatc acccacggca cggacacgct ggaggagacg gcgatgctgt
     1261 gcgacgtggt ccacgacgcc gaggccccga tcgtgatcac cggcgccatc cggcccgcct
     1321 cggcgccggg cgccgacggc ccggccaacc tcagcgacgc cgtcagcgtc gcggccagcg
     1381 aggccgccgc cggcatgggc gtcctggtcg tgttcggggg cgagatccac cacgcccgct
     1441 gcgtccgcaa gaccgacacc acctcgctcg tggccttctc ctcgccccag accgggccgc
     1501 tgggccgcgt gaccgagggc cacccgatca tctggtcgaa gatcccgcgc aacccgccga
     1561 tggacccgaa gaacctgggc aagcgggtgc tggtggtgcc gacgatggcc ggcgacgacg
     1621 gctcgctggc gcacgccgcg ctgagcaccg agccggacgg ggtcatcatc gggacgctgg
     1681 gagcggggca cctggggcca ccgctgctgg acgtctgggc cgaggccgcc aagcggatgc
     1741 ccgtggtggc ctactgccgc cccgaacgcg gcgtggtgct gaactcgacc tacggctacg
     1801 ccggctcgga gcgcgacctg cgcaacaccg ggatcatccc ggccggcttc ctctcgcccc
     1861 aggcggcccg catgaagctg ctggcctgcc tgtccagcga gctctcgatc gacgagatcc
     1921 gctgggcctt ccgccaggac gacggctgag aaaaccaccc gattgcaagg gttcttgcag
     1981 tcgtccggcg aagcccccac catggctcgc ctgatgttca ccgccctact cacctgttcc
     2041 gacgaggact gcaccgagct tgccgcgttc gccggctcgc tcgaggccgc cgacgacgtg
     2101 ttgtgcgagg gctgtggctg cctgatgcag gtcatcgcca tcgagggcgg ccacgagccg
     2161 gccgaggtga tcgacctggc cgcccaccgg acgcgccgcg cgggcgcgcc cggcaggctc
     2221 gccgcctagc gcttcccgac cttcacgctc agggtcttcc gcacggcccg gccgcgcttg
     2281 ggccggagcg tgaccgccag cttgcccttg ccggccttgc gcagcgcccg ggcggccttc
     2341 ttcggcacgc gcagcttcac cagccgcggc ttgcccgcct tgaccctgaa gcgcatcgtg
     2401 gccaccggct tgcgcgcctt gcccgcggcg gacgccttcg cgccggccac cgtgagcgcc
     2461 gccgtgccgg cacagccggt cgggcacgac gcggtcaggg agaccgtcga gcccttggcg
     2521 tcggccgtgg ccgccttcgc cgggaccagc ggcaccagca gctcgtccag gttctccgag
     2581 gccggcgtct tctgaccgct tgcggatccg gacggcgcga ccgcctgccc gggctcgccg
     2641 acgtcggaga tcacggtctc gctggtcttg gtgaacgtga cgtcgccggc ccagtcgtag
     2701 gcgcacttcg tggtgccggc caggtgcgcg ccggcgcaga aggcgccccc gggccccttg
     2761 atgttctgga tgatcttgcc gtagcccacg gcctcgtggg ggatctcgaa ctgcgcgtcc
     2821 aggggcccgc tgcccagcgc cggcccgttg tcggtgtact cgtagggggc cgtcgccgac
     2881 acgcgctcgt cgccccggca ctgccagtcc acgcgcacct ggtcggccac gcggaggttg
     2941 aacgcctcgc cggtgagcgg agccagcagg tcggagtcga tcgtcccctg gccggagggc
     3001 agcggccccg tggtggtgtc gcacgaccgg ctgccgctga acaccttgtt ggcgtagtgg
     3061 cccttggcca cgccggagct gtcggcggtg gcctcgccct gggccacggg cgacatcagg
     3121 tagccgtcgc ggaacgtgat ccggccgagc ttggcgtgca ggtcggagac cagctggacg
     3181 tcggtgtcct ggctctccca cgcggtggcg aagtggtagg cgtggtcgtg cacgagcgtg
     3241 acgtcggcct cgacgtcgta gtgcacgagc ttgaccgagg ccgcctcggc ggccgcgggg
     3301 ccgcaggcca gggccagcgc cgcggtggtc agggcgatgg aggtgtggga gcgcttgggc
     3361 atgtccccac cctgcgcccg gcgcggtccc ctcgcgcccg gggcgcaccc caaacggtcc
     3421 cccaatcccg cgtcggccct acccccgcag cagcgcgcag accgcgtcct cctggacggc
     3481 ccgcaggcgc cgtagcagcg ccccctcggg cgccgggatg ttcagcgacg tggtgaccac
     3541 gcgctggccg tcggagctgg ccgccgccca ctggacgtag ccggggaagt tcccggtgtg
     3601 gccgtagacg gtgccgcacc gcgtgcggta gcggaacagc gccaggccgg ccgagttggc
     3661 ccccgggccg ggcgggctgg agcggccccc ggcgacgaac cggcgctgct cgcgccgcgc
     3721 ggcggtgccg aacaggcgcc cggacacgta gccgcgcatg aacgtggaca ggttccgggg
     3781 cgtcgacacg atggcgcccg acgcccaggc ccccgagggg ctgagcaggg tgctgacgtc
     3841 ctcgggctta ttgcccggct cggtcacgta gccgtggatg aacggccgcg gcagcggcgt
     3901 cgagcgcggg aacgaggtct cgttcagccc cagcggcccg aacacgaagt cgtcgagcag
     3961 cgcgccgtag gggcggccgg tgatccgctc cgcgatcagc ccgatgacga tgttgtcggt
     4021 g
//
LOCUS       DASUPW010000506         4092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_257046, whole genome shotgun sequence.
ACCESSION   DASUPW010000506 DASUPW010000000
VERSION     DASUPW010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257046"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VFY44_11360"
     CDS             <1..553
                     /locus_tag="VFY44_11360"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911083.1"
                     /translation="DGDEEAQLSFAGARQGLEHREVERLGVVDVGGGSTELATGGADG
                     PADWTASVPVGSSVLCRSHPAGDPPQDAHVDAWHAAAAEALDGVQPPAVDLSLAVGGT
                     ATSLHRLTGPRLTTTSLRRALERLCAHPAAEAARRLDLDPERVLLLPAGITVLMAAAA
                     RLGSELLPARGGLRESALLELAA"
     gene            553..1029
                     /locus_tag="VFY44_11365"
     CDS             553..1029
                     /locus_tag="VFY44_11365"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX5911084.1"
                     /translation="MAKARPIRGLSPDTRFRDAAAVAVSVRAEEVFSFADAALDLTDI
                     EGVHDMRVATRRLRAALEVFATCFPRKQHAAALAEVKDLADRLGERRDPDVAIEELEA
                     LAAEFAPEDRPGVAGLVEELRAEQAAANETVARALADLHESGLAGRLQELAQAARS"
     gene            1026..1613
                     /locus_tag="VFY44_11370"
     CDS             1026..1613
                     /locus_tag="VFY44_11370"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX5911085.1"
                     /translation="MKAKRVKGLDPDGAFADDARKIVKVRVDELYALAPKALDVDRPK
                     KLHDLRIAAKRLRYVLEIARPALGKGATDGVRTAKALQQLLGDIHDCDEMLPRVQAHA
                     QRLRARDVEEVRRRAGRNARDLKPGLAPAREGADHYRGLETLAAHLAARRQVLFARFE
                     REWIRLEARDFRGTLLAAISPPEPEAPAPPDGAGP"
     gene            complement(1561..1989)
                     /locus_tag="VFY44_11375"
     CDS             complement(1561..1989)
                     /locus_tag="VFY44_11375"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5911086.1"
                     /translation="MLRHADAEDGSPDAERPLSEKGRRQAEAAGRALAAMDVRIDACV
                     TSPKVRARDTAALACKALGIEPEEDLRLEGGPFDPREVAEDRGEHVLLVGHDPDFSLA
                     VNRLTGAQVRMKKGAVAAIDRGELMVLLRPAELALLAREG"
     gene            2032..>4092
                     /gene="ppk1"
                     /locus_tag="VFY44_11380"
     CDS             2032..>4092
                     /gene="ppk1"
                     /locus_tag="VFY44_11380"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEX5911087.1"
                     /translation="MTVTDSTTPPDLDSTSLYANRELSWLEFNERVLELAEDERTPLM
                     ERVKFLAIYTSNLDEFEMVRVAGLHDQVDAGIDVRKADGLSPSETIERIAGRRRELGE
                     RHSRQWTDHVRPALAGEGIRILDGDDLTADESERLDRIFGEQIFPVLTPLAVGPGRPF
                     PYISNLSLSLGVWVHDPVNDTRLFARVKVPREVLPRFVDLGDGAFVPQEVAIARNLDT
                     LFPGMEVQGHAVFRVTRDADFEVSDEADDLLRAVEDELRARRFGEVVRLEVQAGTDPD
                     LRDFLVEQLGIEESECVDVHGLLDLGDLWQLHGLDGYAHLHEEAWTPVNAGPFASADG
                     KADVMGAMRTSDLLVHHPYDSFVGSVERLVEQAVRDPQVLAIKMTVYRTSDDSRLVPL
                     LIEAAERGKQAVCLVELKARFDERRNIGWARSLEEAGAHVVHGLPGLKTHAKALLIVR
                     REGNGVRHYVHVGTGNYHAKTARLYEDFGLFTTNRQFAGDVAALFNVLTGAARPLGYQ
                     KALVAPEGMRTGLLDQIEQTVEAHRQGRDARIILKMNSLVDRRCIKALYEASQAGVPV
                     DINVRGICCLRPGVPGVSENIRVVSVVGRFLEHSRVYLFQRGEEHRAFMGSADLMPRN
                     LDTRVELLMPVESPQLLAEVEDTLGRCLADNTFAWDLGADGEWTRRSGGERSVHAELM
                     ERALS"
BASE COUNT          557 a         1454 c         1556 g          525 t
ORIGIN      
        1 ggacggcgac gaggaggcgc agctgtcctt cgcgggcgcg cgccagggcc tggagcaccg
       61 tgaggtcgag cgcctgggcg tggtggacgt gggcggcggc tccaccgagc tggcgaccgg
      121 gggcgccgac ggccccgccg actggaccgc gtcggtgccg gtgggctcca gcgtgctgtg
      181 ccgcagtcac ccggccggcg acccgccgca ggacgcgcac gtggacgcct ggcacgccgc
      241 ggcggcggag gccctggacg gggtgcagcc gcccgccgtg gacctgtcgc tggccgtggg
      301 cggcacggcc acctcgctgc accgcctgac cggtccgcgc ctgaccacga cgtcgctgcg
      361 acgggcgctg gagcgcctgt gcgcgcaccc ggccgcagag gcggcgcggc ggctggacct
      421 ggaccccgag cgcgtgctgc tgctgcccgc gggcatcacc gtgctgatgg ccgctgccgc
      481 gcggctgggc agcgagctgc tgccggcccg cggtgggctg cgcgagagcg cgctgctgga
      541 gctggcggcc tgatggcgaa ggccaggccg atccggggcc tgtcccccga cacccgcttc
      601 cgcgacgccg ccgccgtggc cgtgtcggta cgcgccgagg aggtgttctc gttcgcggac
      661 gccgccctgg acctgacgga catcgagggc gtgcacgaca tgcgcgtggc cacgcgccgg
      721 ctgcgcgccg cgctcgaggt gttcgccacc tgcttcccgc gcaagcagca cgcagcggcg
      781 ctggccgagg tcaaggacct ggccgaccgg ctgggcgagc ggcgcgaccc cgacgtggcg
      841 atcgaggagc tggaggcgct ggcggcggag ttcgcccccg aggaccgccc cggggtggcc
      901 gggctggtcg aggagctgcg ggccgagcag gccgcggcca acgagaccgt ggcccgggcg
      961 ctggccgacc tgcacgagag cgggctggcc ggccggctgc aggagctggc gcaggcggcg
     1021 cgctcgtgaa ggccaagcgg gtcaaggggc tggaccccga cggcgcgttc gccgacgacg
     1081 cacggaagat cgtgaaggtg cgcgtggacg agctgtacgc gctggcgccc aaggcgctgg
     1141 acgtggaccg gcccaagaag ctgcacgacc tgcggatcgc cgccaagcgg ctgcgctacg
     1201 tgctggagat cgcgcggccg gcgctgggca agggcgccac cgacggcgtg cgcacggcca
     1261 aggccctcca gcagctgctg ggcgacatcc acgactgcga cgagatgctg ccgcgcgtgc
     1321 aggcgcacgc gcagcggctg cgggcgcgcg acgtcgagga ggtgcgccgc cgcgcggggc
     1381 gcaacgcgcg cgacctcaag cccgggctgg cgcccgccag ggagggcgcg gaccactacc
     1441 gcgggctgga gacgctggcc gcccacctgg ccgcgcgccg ccaggtgctg ttcgcccgct
     1501 tcgagcgcga gtggatcagg ctcgaggcgc gcgacttccg cggcacgctg ctggccgcga
     1561 tcagccctcc cgagccagaa gcgccagctc cgccggacgg agcaggacca tgagctcgcc
     1621 gcggtcgatg gccgccacgg cccccttctt catgcgcacc tgtgcgccgg tcagccggtt
     1681 gaccgccagc gagaagtcgg gatcgtggcc caccagcagc acgtgctcgc cacgatcctc
     1741 ggccacctcg cgcgggtcga acgggccgcc ctccagccgc aagtcctcct cgggctcgat
     1801 gccgagcgcc ttgcaggcca gcgcggcggt gtcgcgggcc cgtaccttgg ggctggtgac
     1861 gcacgcgtcg atccgcacgt ccatcgccgc cagcgcgcgg ccggcggcct ccgcctgccg
     1921 gcggcccttc tccgacagcg ggcgctcggc gtccggggac ccgtcctcgg cgtctgcgtg
     1981 gcgcaacatc cagatcacga ctggccaagc tagagcagcg aggatttaca agtgaccgtg
     2041 accgactcca ccaccccgcc ggacctggac tcgacgtccc tgtacgccaa ccgcgagctg
     2101 tcgtggctgg agttcaacga gcgcgtgctg gagctggcgg aggacgagcg cacgccgctg
     2161 atggagcggg tcaagttcct ggccatctac acctcgaacc tggacgagtt cgagatggtg
     2221 cgcgtggccg gcctgcacga ccaggtggac gccggcatcg acgtgcgcaa ggccgacggg
     2281 ctgtcgccgt cggagacgat cgagcggatc gccggccgcc gccgcgagct gggcgagcgc
     2341 cactcgcgcc agtggaccga ccacgtccgc ccggcgctgg ccggggaggg catccggatc
     2401 ctggacggcg acgacctgac cgccgacgag tccgagcgcc tggaccgcat cttcggcgag
     2461 cagatcttcc ccgtgctgac cccgctggcc gtggggcccg ggcggccctt cccctacatc
     2521 tcgaacctgt cgctgagcct gggcgtgtgg gtgcacgacc cggtcaacga cacgcgcctg
     2581 ttcgcgcggg tgaaggtgcc gcgcgaggtg ctgccacggt tcgtcgactt gggcgacggc
     2641 gcgttcgtgc cccaggaggt ggccatcgcg cgcaacctgg acaccctctt cccgggcatg
     2701 gaggtccagg gccacgcggt gttccgggtt acccgcgacg ccgacttcga ggtgtccgac
     2761 gaggccgacg acctcctgcg cgcggtcgag gacgagctcc gcgcccgccg cttcggcgag
     2821 gtcgtccggc tggaggtgca ggccggcacc gatcctgacc tgcgcgactt cctggtcgag
     2881 cagctgggca tcgaggagag cgaatgcgtg gacgtccacg gcctgctgga cctgggcgac
     2941 ctgtggcagc tgcatggcct ggacggctac gcgcacctgc acgaggaggc gtggacgccg
     3001 gtgaacgccg gcccgttcgc gtccgccgac ggcaaggcgg acgtgatggg cgcgatgcgc
     3061 acgtccgacc tgctggtgca ccatccatac gactcgttcg tgggcagcgt ggagcgcctg
     3121 gtggagcagg ccgtgcgcga cccccaggtg ctggcgatca agatgaccgt gtaccgcacc
     3181 tccgacgact cgcggctggt gccgctgctg atcgaggccg ctgagcgggg caagcaggcc
     3241 gtgtgcctgg tggagctcaa ggcccgcttc gacgagcgcc ggaacatcgg ctgggcgcgg
     3301 tcgctggaag aggcgggcgc gcacgtggtg cacggcctgc cgggcctgaa gacccacgcc
     3361 aaggcgctgc tgatcgtgcg ccgcgagggc aacggcgtgc gccactacgt gcacgtgggc
     3421 accggcaact accacgccaa gaccgcccgg ctgtacgagg acttcggcct gttcaccacc
     3481 aaccgccagt tcgccggcga cgtggccgcc ctgttcaacg tgctgaccgg cgccgcccgc
     3541 ccgctgggct accagaaggc cctggtggca cccgagggca tgcgcacggg gctgctggac
     3601 cagatcgagc agaccgtcga ggcccaccgc cagggtcgtg acgcgcggat catcctgaag
     3661 atgaactcgc tggtggaccg gcgctgcatc aaggcgctgt acgaggcctc gcaggccggc
     3721 gtgccggtgg acatcaacgt gcgtggcatc tgctgcctgc gcccgggcgt gcccggcgtc
     3781 agcgagaaca tccgcgtggt gtcggtggtg ggccggttcc tcgagcactc gcgggtctac
     3841 ctgttccagc gcggcgagga gcaccgcgcg ttcatgggct cggccgacct gatgccacgc
     3901 aacctggaca cgcgcgtgga gctgctgatg ccggtcgagt cgccgcagct gctggccgag
     3961 gtcgaggaca cgctggggcg gtgcctggcc gataacacgt tcgcctggga cctgggcgcg
     4021 gacggcgagt ggacacggcg cagcggcggt gagcgctcgg tgcacgccga gctaatggag
     4081 cgcgcgctct cc
//
LOCUS       DASUPW010000507         2306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_257249, whole genome shotgun sequence.
ACCESSION   DASUPW010000507 DASUPW010000000
VERSION     DASUPW010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257249"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1201)
                     /locus_tag="VFY44_11385"
     CDS             complement(134..1201)
                     /locus_tag="VFY44_11385"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEX5911088.1"
                     /translation="MGQTRAGRLAEVLERRELDSLLVSNLVNVRWLTGFTGTNGAAVV
                     TRDRRLFLTDFRYTEQAEEQVDPSFERLPAGRDLVGDLASQLTGRAGFDDNHMSVATH
                     RKLLDKVADGVELVAAGGAVEELREVKDQGELTRIAAAQELASAALERILERGLVGRT
                     ERAVALDLEQEMRQAGATGPSFPSIVASGPHGALPHAVPRDVEIAAGQLVVLDWGALL
                     DGYCSDCTRTVATGPLEDDAAEAYELVRRAQQTGLEAVREGASCKGVDAAARDVIAAA
                     GFGDQFGHSLGHGVGLEVHEGPTLSMRSEGDLAAGNVVSVEPGVYLPGRFGIRIEDLV
                     AVTEDGADVFTLLTKELVTVD"
     gene            complement(1201..1641)
                     /locus_tag="VFY44_11390"
     CDS             complement(1201..1641)
                     /locus_tag="VFY44_11390"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX5911089.1"
                     /translation="MSRKRIGVLHGVNLDQLGKRDPDVYGTFTLGELETRVHQWGYEQ
                     DSEVTFWQTNHEGEYCEMLHEARDRVDGLILNPGAWTHYSYAIRDALEVCALPAVEIH
                     ISEVDSRDDWRRISVIRDLCVGRVQGEGAEGYRRALQILREALG"
     gene            complement(1638..>2306)
                     /locus_tag="VFY44_11395"
     CDS             complement(1638..>2306)
                     /locus_tag="VFY44_11395"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HEX5911090.1"
                     /translation="VPTTLVAQVDSAYGGKTGVDLPEGKNYAGVYHQPTAVVCDPAVL
                     DTLPEAEKAAGYAEVVKTALIAGGPLWARVRGGGPVDDETILGCIRTKLDVVAADERD
                     EGRRQVLNLGHTVGHAIEAATGYSRYRHGEAVAVGLLAALRLSGREALREEVAALLSG
                     HGLPLTFEGASVDQVVALTARDKKRSGDTVPFVLVQAPGEVTPGHRIAPAALEEAVAE
                     VHAG"
BASE COUNT          302 a          898 c          784 g          322 t
ORIGIN      
        1 gcaggcccgg gccgtgctcc gccccggtca gtggcccgag ctgctcaagc gcctggacga
       61 gatcgagaac ccggtcgtgc cgacgaagcc gtcgaagtac gcgatcccgg tgaaaaaact
      121 caagaagctc ccgtcagtcg acggtaacta gctccttcgt cagcagcgtg aagacgtcgg
      181 cgccgtcttc ggtgaccgcc acgaggtcct cgatgcggat gccgaaccgg ccgggcaggt
      241 agacgccggg ctccacgctg accacgttgc cggccgccag gtcgccctcg gagcgcatcg
      301 agagcgtggg cccctcgtgc acctccaggc ccaccccgtg ccccaggctg tggccgaact
      361 ggtcgccgaa gcccgcggcg gcgatcacgt cgcgcgcggc cgcgtcgacc cccttgcacg
      421 acgcgccctc acggacggcc tccagcccgg tctgctgggc gcgcctgacc agctcgtagg
      481 cctccgccgc gtcgtcctcc agcggccccg tggcgaccgt gcgcgtgcag tccgagcagt
      541 agccgtccag cagcgcgccc cagtccagga ccaccagctg cccggccgcg atctccacgt
      601 cgcgcgggac ggcatgtggc agggccccgt ggggcccgga ggcgacgatg gacgggaacg
      661 agggcccggt cgcgcccgcc tggcgcatct cctgctcgag gtccagggcg acggcgcgct
      721 cggtgcgccc gaccaggccg cgctcgagga tccgctccaa ggcggcgctg gccagctcct
      781 gggccgcggc gatccgtgtc agctccccct ggtccttgac ctcgcgcagc tcctcgacgg
      841 caccgccggc ggccacgagc tcgacgccgt cggccacctt gtccagcagc ttgcggtggg
      901 tggccacgct catgtggttg tcgtcgaacc ccgcgcggcc ggtcagctgc gaggccaggt
      961 cgcccaccag gtcgcgcccg gccgggagcc gctcgaacga agggtcgacc tgctcctcgg
     1021 cctgctcggt gtagcggaag tcggtcagga acaggcgccg gtccctggtg accaccgccg
     1081 cgccgttggt gccggtgaag ccggtcagcc agcgcacgtt gaccaggttc gagaccagca
     1141 gcgagtccag ctcgcgccgc tccagcacct ctgccagccg tccggcccgc gtctgtccca
     1201 tcagcccagg gcctccctca ggatctgcag cgcccgccgg tagccctccg cgccctcacc
     1261 ctgcacgcgc cccacgcaca ggtcgcggat caccgagatg cggcgccagt cgtcacggct
     1321 gtccacctcg gagatgtgga tctcgacggc gggcagcgcg cagacctcca gcgcgtcgcg
     1381 gatggcgtac gagtagtgcg tccacgcgcc cgggttcagg atcaggccgt cgacccggtc
     1441 gcgggcctcg tgcagcatct cgcagtactc gccctcgtgg ttggtctgcc agaacgtgac
     1501 ctcggagtcc tgctcgtagc cccactggtg cacccgcgtc tccagctcgc cgagcgtgaa
     1561 cgtgccgtac acgtcggggt cgcgcttgcc cagctggtcc aggttcactc cgtggagcac
     1621 cccgatccgc ttgcggctca tcccgcgtgc acctcggcca cggcctcctc cagcgccgcg
     1681 ggcgcgatcc ggtggcccgg ggtgacctcg cccggggcct gcacgagcac gaacgggacg
     1741 gtgtcgccgg agcgcttctt gtcgcgcgcg gtcagcgcga ccacctggtc caccgaggcg
     1801 ccctcgaagg tcagcggcag cccgtggccg gacagcaacg ccgccacctc ctcgcgcagc
     1861 gcctcgcggc ccgacagccg cagtgcggcc agcagcccca ccgccaccgc ctcgccgtgg
     1921 cggtagcgcg agtagccggt cgcggcctcg atggcgtggc ccacggtgtg acccaggttc
     1981 aggacctggc gccggccctc gtcgcgctcg tcggcggcca caacgtcgag cttggtgcgg
     2041 atgcagccca ggatcgtctc gtcgtccacg gggccgccgc cccggacgcg ggcccacagc
     2101 ggtccgccgg cgatcagggc ggtcttcacc acctcagcgt atcccgccgc cttctcggcc
     2161 tcgggcagcg tgtccagcac cgccgggtcg cacacgaccg cggtcggctg gtggtagacg
     2221 cccgcgtagt tcttgccctc gggcaggtcg acgccggtct tcccgccgta ggccgagtcc
     2281 acctgggcga cgagcgtcgt gggcac
//
LOCUS       DASUPW010000508         2966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_533825, whole genome shotgun sequence.
ACCESSION   DASUPW010000508 DASUPW010000000
VERSION     DASUPW010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533825"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VFY44_11400"
     CDS             complement(<1..219)
                     /locus_tag="VFY44_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911091.1"
                     /translation="MTSLSDFTNEPVAELRRAPAREALTAALRDLDATLPLEVPVLIG
                     GDRGAATGLTSTDPGDPERVVATAGMGGA"
     gene            complement(397..1737)
                     /locus_tag="VFY44_11405"
     CDS             complement(397..1737)
                     /locus_tag="VFY44_11405"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX5911092.1"
                     /translation="MDLEPSIQAIGTEIHDALPAPSRNPVKVVDAKAMEMASRDEELR
                     SALFRLVDVTPACRSTADLSRHLVAYLEEVPDRPPALDAALRAAHAPGGARALGRATA
                     TGVRHMAHRFIAGETPKKALGEFRRLWEAGVASSVDLLGEATVTTAEADHYAARCLDA
                     LETVAPAAARWPAREALERDSLGGIARANVSVKISALTPHMRPEAPQVGRDDAGARLR
                     PLLRRAKELGAYLHIDMESVDSLETTFELIFGVLGEDEFRDGPESGLVLQAYLRESPD
                     QLEQVLDFVRRSGRTTPLTVRLVKGAYWDHELVEARQHGWSVPVFEVKADSDRNFELL
                     TRGLLEARAGGDGVRVAVASHNLRSVAHAVACNRALGGDDGDLELQVLRGLGDELQHA
                     LSAQGLRVRAYCPVGDLVAGMAYLVRRLLENTSNDSFLGDQQRGVPIGELLAAP"
     gene            1792..>2966
                     /locus_tag="VFY44_11410"
     CDS             1792..>2966
                     /locus_tag="VFY44_11410"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX5911093.1"
                     /translation="MRALLAGAAAALFLLAGCGDPPRATFTVTPDAPRQAGQELRFLS
                     DVSCSRGATRRMAWDVDGDGRFERGPEDCDEAEVEDFRHTYARPGTYKVSTWAQVPRP
                     VFVFGFLAYADGYSSQRLVIGSDGPPAANQPPVPDFTANPNPGYTERPVNLDAAPSRD
                     PDGRIVKYEWDRDGDGTWDANGPTTSYATDRAGTLQVRLRVTDDDGATATTERAVPIV
                     DGVPPGESLRAAAAARAARATRFSTVLSGKLTSQGRRFVAGATFSGTGIKARGRMRLA
                     GLPSPLRGKRRARWAATLTTKQKGTTRAARLAASGYLLLDLRGGDRLCMNGRLGGRLG
                     SPFTGRLAVVGGTGKGRRIRGTARLTVPVVRRARLRGTLNLARSKKARKLPRPCRALV
                     "
BASE COUNT          394 a         1133 c         1078 g          361 t
ORIGIN      
        1 cgcgccgccc atgccggcgg tggccacgac ccgctcgggg tcgccggggt cggtcgaggt
       61 caggcccgtg gccgccccgc ggtcgccgcc gatcaggacc ggcacctcca acggaagggt
      121 ggcgtccagg tcccggagcg cggcggtcag tgcctcccgg gcgggcgcgc gccgcagctc
      181 ggcgacgggc tcgttggtga agtcggagag ggaggtcatg cgcagttggt caccgtcgca
      241 accctcccgt tacccgcgac gaatacccgg acatcgccgt gcgcacttga ccacctatac
      301 gggggtcaac tgcgcacgtc ttcgaggggc gtcctaccca gccgagccgt tgcgcctgac
      361 ccgcaggggg caccggcggc agaaccccag ccaggctcac ggcgccgcca gcagctcgcc
      421 gatgggcacg ccgcgctgct ggtcacccag gaacgagtcg ttggaggtgt tctccagcag
      481 gcgccgcacc aggtaggcca tgcccgccac caggtcgccg accgggcagt aggcgcgcac
      541 ccgcaggccc tgggcggaca gcgcgtgctg gagctcgtcg cccaggccgc gcaggacctg
      601 cagctccagg tccccgtcgt cgccgcccag cgcgcggttg caggcgaccg cgtgggccac
      661 cgagcgcagg ttgtgcgagg ccaccgccac ccggaccccg tccccgccgg cccgcgcctc
      721 gagcagcccg cgcgtcagca gctcgaagtt tcggtccgaa tcggccttga cctcgaacac
      781 cggcacgctc cagccgtgct ggcgggcctc gaccagctcg tggtcccagt aggcgccctt
      841 gaccaggcgc acggtcagcg gcgtggtgcg gcccgagcgg cgcacgaaat ccagcacctg
      901 ctccagctga tcgggagact cgcgcaggta ggcctgtagc accagaccgg actcggggcc
      961 gtcgcggaac tcgtcctcgc ccagcacgcc gaagatcagc tcgaaggtcg tctccagcga
     1021 gtcgaccgac tccatgtcga tgtgcaggta ggcccccagc tccttcgccc gccgcagcag
     1081 cgggcgaagc cgggctccgg cgtcgtcgcg gcccacctgc ggggcctcgg ggcgcatgtg
     1141 gggcgtcagc gccgagatct tcacggacac gttggcgcgc gcgatcccgc cgagcgagtc
     1201 gcgctccagc gcctcccgcg ccggccacct tgccgcggcc ggggccacgg tctcgagcgc
     1261 gtcgaggcag cgggccgcgt agtggtcggc ctccgcggtg gtcacggtcg cctcgccgag
     1321 caggtcgacc gagctcgcca ccccggcctc ccacaggcgc cggaactcgc ccagcgcctt
     1381 cttgggcgtc tcgccggcga tgaaccggtg cgccatgtgg cgcacgcccg tcgccgtggc
     1441 gcggcccagc gcgcgcgctc ctccgggggc gtgcgccgcc cgcagcgcgg cgtccagggc
     1501 gggcggccgg tcgggcacct cctcgaggta ggcgaccagg tggcgcgaga ggtcggccgt
     1561 cgagcggcac gcgggcgtca cgtccaccag ccggaagagc gccgagcgca gctcctcgtc
     1621 gcgcgaggcc atctccatgg ccttcgcgtc caccaccttc accggattgc gggacggcgc
     1681 aggcagcgca tcgtggatct cggtgccgat cgcctggatc gagggctcga ggtccacaag
     1741 gagacggtac ccgggttgcg aaacggtacg aagccgtgca ggattgccgc gatgcgagca
     1801 ctcctggcag gcgcggcggc ggccctcttc ctcctggccg ggtgcgggga cccgccgcgg
     1861 gccacgttca ccgtgacgcc cgacgccccc cggcaggccg ggcaggagct gcgcttcctc
     1921 tccgacgtgt cctgcagtcg cggcgccacg cgcaggatgg cctgggacgt ggacggcgac
     1981 gggaggttcg agcgcgggcc cgaggactgc gacgaagccg aggtggagga cttccgccac
     2041 acctatgccc ggcccgggac ctacaaggtg agcacgtggg cgcaggtacc gcggcccgtg
     2101 ttcgtcttcg gcttcctcgc ctatgccgac gggtactcgt cccagcgcct ggtgatcggc
     2161 tcggacgggc cgcccgccgc caaccagccc cccgtccccg acttcacggc gaaccccaac
     2221 cccggctaca cggagcgtcc cgtgaacctg gacgccgcgc cctcgcgcga cccggacggc
     2281 cggatcgtca agtacgagtg ggaccgcgac ggcgacggga cctgggacgc gaacgggccc
     2341 accacgagct acgccaccga ccgcgccggc acgctccagg tccgtctgcg cgtgaccgac
     2401 gacgacggcg cgacagccac caccgagcgg gccgtaccca tcgtggacgg cgttccgccc
     2461 ggcgagtcgc tgcgcgccgc cgcggccgcc cgggccgcga gggccacgcg cttctcgacg
     2521 gtcctgtcgg gcaagctgac ctcgcagggc aggcggttcg tggccggcgc gacgttctcg
     2581 ggcacgggga tcaaggcccg cggccgcatg cgcctggccg ggctgccctc gcccctgcgc
     2641 ggcaagcgcc gcgcccgctg ggccgcgacc ctgaccacga agcagaaggg gacgaccagg
     2701 gccgcgcggc tggccgcgtc gggctacctg ctgctggacc tgcgcggcgg cgatcgcctg
     2761 tgcatgaacg gccgcctggg agggcggctg ggctctccct tcaccggccg cctggcggtc
     2821 gtgggcggca ccggcaaggg ccggcggatc cgcggcacgg cgcggctgac cgtcccggtc
     2881 gtacggaggg cgaggctccg cggcacgctg aacctggccc ggtcgaagaa ggcgcgcaag
     2941 ctgccccggc cctgccgcgc tctcgt
//
LOCUS       DASUPW010000509         2627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_257468, whole genome shotgun sequence.
ACCESSION   DASUPW010000509 DASUPW010000000
VERSION     DASUPW010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257468"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1752
                     /locus_tag="VFY44_11415"
     CDS             <1..1752
                     /locus_tag="VFY44_11415"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5911094.1"
                     /translation="LVSLLLGSFWSRRNAADALRRERRFHALLRNSSDLVVVIDPDTL
                     TIRYATPAIERMLGYPPESVLGTPLAQLTHPEDRGALTDASLAVRASDDGHETDRWRA
                     MHHEGGVVDVEASWLDLTDDASVQGLVVTVRDVGERTNLEDQLRHQAFHDPLTALPNR
                     SLFEDRVRHAVARTRRHGRGMAVLFVDLDDFKTVNDSLGHAAGDELLRQVAERLDGWV
                     RSSDTVARLGGDEFAVLVEEPDDPDEAREIADRIHAGLEQPFAIDGHELFTRASVGIA
                     PAESGATSEELMRNADTAMYVAKAAGKGRTEVFRATMHMEVQRRLQLSGDLRRALDNG
                     ELHVQYQPLVDLPSGRVLGAEALARWNHPKLGQVPPVDFIPVAEETGLIVPLGGWVLA
                     EACGQAVRWHAGSDSDPIYVSVNVSPRQFRQPGTVVEQVKAALDGSGLDPTFLVLEIT
                     ESVLMQDREVIRRELTALRDLGVRVAIDDFGTGYSALTYLRDFPIDMVKMDQSFVNDL
                     SAGRGDQALVRSVVELGEALDMQIVAEGIERRDQLDHLSDMRCSVGQGYYFARPLDGD
                     GMGRLIDNQLAPTPPAP"
     gene            complement(1756..2220)
                     /locus_tag="VFY44_11420"
     CDS             complement(1756..2220)
                     /locus_tag="VFY44_11420"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX5911095.1"
                     /translation="MAPTTINGIEELQGLVGETVGPSDWRTVTQEMINGFAEISGDHQ
                     WIHVDVDRAKAESPFGGPIAHGNLTLATIDGFRDGLLVQDGFKLGVNYGWNKVRFPAP
                     VPVDSNIRASIEIVSVDDVGGGWHQVVQRWTVEIEGGEKPVCVADSVTRVMA"
     gene            2344..>2627
                     /locus_tag="VFY44_11425"
     CDS             2344..>2627
                     /locus_tag="VFY44_11425"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX5911096.1"
                     /translation="MLALALAAAPAAQARSADTAALQVALRAVHLYGGGVDGIAGPAT
                     RRAVRRFQRRHRLAADGVAGPRTRRALGRRGRPRLGSRMLRTGRRGWDVAA"
BASE COUNT          364 a          984 c          923 g          356 t
ORIGIN      
        1 ctggtctcgc tgctgctggg gagtttctgg tcgcgacgca acgccgccga cgccctccgg
       61 cgggagcgcc ggttccacgc gctgctgcgc aactcctccg acctggtcgt ggtaatcgac
      121 cccgacacgc tcacgatccg ctacgccacc cccgccatcg agcgcatgct gggctatccc
      181 cccgagtccg tcctgggcac ccccctggcc cagctgaccc atccggagga ccgcggcgcg
      241 ctgaccgacg cgtcgctggc agtgcgggcc agcgacgacg ggcacgagac cgaccgctgg
      301 cgcgcgatgc accacgaggg cggcgtggtc gacgtcgagg ccagctggct cgacctgacc
      361 gacgacgcgt cggtccaggg cctggtggtc accgtccgcg acgtcggcga gcgcaccaac
      421 ctcgaggacc agctgcgcca ccaggccttc cacgacccgc tcaccgccct tcccaaccgc
      481 tcactgttcg aggaccgcgt gcgccacgcg gtggcccgca ccaggcgcca cgggcgcggc
      541 atggccgtgc tgttcgtgga cctggacgac ttcaagaccg tcaacgactc gctcggccac
      601 gccgccggcg acgagctcct gcgccaggtc gccgagcgcc tggacggctg ggtccgcagc
      661 tcggacaccg tcgcccgcct gggcggcgac gagttcgccg tcctcgtcga ggagcccgac
      721 gaccccgacg aggcccgcga gatcgccgac cgcatccacg ccggcctgga gcagccgttc
      781 gcgatcgacg gccacgagct gttcacccgc gccagcgtgg gcatcgcgcc cgccgagtcg
      841 ggggcgacca gcgaggagct gatgcgcaac gccgacaccg ccatgtacgt ggccaaggcc
      901 gccggcaagg gccggaccga ggtcttccgg gccacgatgc acatggaggt ccagcgccgg
      961 ctgcagctca gcggcgacct gcgccgggcc ctggacaacg gcgagctgca cgtgcagtac
     1021 cagccgctcg tggaccttcc ctccgggcgc gtgctcggag ccgaggcgct ggcgcgctgg
     1081 aaccacccga agctgggcca ggtcccaccc gtcgacttca tccccgtggc cgaggagacc
     1141 ggcctgatag tgcccctggg cggctgggtg ctggccgagg cctgcggcca ggcggtgcgc
     1201 tggcacgcgg gaagcgactc cgacccgatc tacgtcagcg tgaacgtctc gccgcgccag
     1261 ttccgccagc cgggcacggt ggtcgagcag gtgaaggccg cgctggacgg cagcgggctg
     1321 gacccgacgt tcctggtgct ggagatcacc gagtcggtgc tcatgcagga ccgcgaggtc
     1381 atccgccggg agctcacggc gctacgggac ctcggcgtgc gcgtcgccat cgacgacttc
     1441 ggcaccggct actcggcgct gacctacctg cgcgacttcc cgatcgacat ggtcaagatg
     1501 gaccagtcgt tcgtgaacga cctgtccgcc gggcgcggcg accaggcgct ggtgcgctcg
     1561 gtggtcgagc tcggcgaggc gctggacatg cagatcgtgg ccgagggcat cgagcgccgc
     1621 gaccagctgg accacctgtc cgacatgcgc tgcagcgtcg gccagggcta ctacttcgcg
     1681 cgtcccctgg acggggacgg catgggccgg ctgatcgaca accagctggc ccccacgcct
     1741 cccgctccct gagcgtcagg ccatgacgcg cgtgaccgag tcggccacgc agaccggctt
     1801 ctcgccgcct tcgatctcga ccgtccagcg ctgcacgacc tggtgccagc caccgccgac
     1861 gtcgtcgacc gagacgatct cgatcgaggc gcggatgttc gagtccaccg gcaccggggc
     1921 cgggaagcgc accttgttcc agccgtagtt gacgccgagc ttgaagccgt cctgcaccag
     1981 caggccgtcg cggaagccgt cgatggtggc cagcgtcagg ttgccgtggg cgatcggccc
     2041 gccgaacggg ctctcggcct tcgcgcgatc cacgtccacg tggatccact ggtggtcccc
     2101 ggagatctcg gcgaagccgt tgatcatctc ctgggtcacg gtgcgccagt cgctcgggcc
     2161 gacggtctcg ccgaccaggc cttgcagttc ctcgatgccg ttgatggtgg tgggagccat
     2221 gaggcgagcg tacccgctgg gtgcagcgtt tcatgcaccc cctcgggaat ccgatcgccc
     2281 gtggcgagtg ttggagaggc aatggaaccc aggggtaccc gacttctggc cgcgatcgcg
     2341 gcagtgctgg cgctggcgct ggccgcggcg ccggccgcgc aggcacgcag cgcagacacc
     2401 gccgcgctgc aggtggccct ccgcgcggtc cacctctacg gcggcggcgt cgacggcatc
     2461 gccggcccgg ccacccgccg ggcggtgcgc cgcttccagc gccgccaccg gctcgcagcc
     2521 gacggcgtgg cggggccccg cacccggcgg gcgctcggcc gccgcggccg cccccggctg
     2581 ggaagccgca tgctgcgcac gggccgccgg ggctgggacg tcgcggc
//
LOCUS       DASUPW010000510         4104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_118102, whole genome shotgun sequence.
ACCESSION   DASUPW010000510 DASUPW010000000
VERSION     DASUPW010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118102"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VFY44_11430"
     CDS             <1..433
                     /locus_tag="VFY44_11430"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5911097.1"
                     /translation="PRTIVPPEATAKVTMRLAPGQDPAVMSEVLLGLLREGLPEGAEL
                     DTRVQTALPALFEVDQPAMQLAARALEKACGRPPAFVRSGGSIPIVAEMAAQGYPVIV
                     GGFGLPDDAIHAPNESYALRSLEWGEAAARELFEALAELPR"
     gene            433..1395
                     /locus_tag="VFY44_11435"
     CDS             433..1395
                     /locus_tag="VFY44_11435"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5911098.1"
                     /translation="MAWTENPNLDTAAVTSPTTAAAGFHHTLDGYAPTPVRELADGTL
                     VKDESGRLGLPAFKILGASWAVERALAGAPATTTLVAASAGNHGRAVAHEAARRGLEC
                     RVFLPARALEARREAIAGEGAAVEVVDGSYEECVALARAAGERGDTLEIADVGDSGPA
                     RDVIDGYATLFDEAAHQADFDVLVVPIGVGSLGAAAARFGARAGIPVVGAEPEAAACL
                     TASLAAGEPTAVPTPGTTMAGLDCAEVSAAAWPDLRAGVSGTVTVSDHEAADAQRELE
                     AAGMYIGESGAAPLAALRQGGLGPDGARVLLVATEGRTGAPHPG"
     gene            1405..2670
                     /locus_tag="VFY44_11440"
     CDS             1405..2670
                     /locus_tag="VFY44_11440"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5911099.1"
                     /translation="MPRVPRLPLIPDPLRRIRVPRDLEAVTAVGEEADPADAGMTRDS
                     VEQIWSDAVTWYRSGVHPAMQVCVRRHGTVVLDRAIGHARGNGPGDAPDEPKVPVATD
                     TPFCVYSASKAITAFVVHGLIEQGKVGLDEPVAEYIPGYGINRKHAITVGHVLAHRAG
                     VPNMPGHSFDLDKMGDREFIRGALVDAEPFAKPGRMLAYHAVSGGYILGEIVHQVTGK
                     DIRTVLAESILDPLGFRWTNYGVAEQDLAALGTDYVTGPPLLPPASQLLTRALGVPLD
                     TIVEWSRDPRFRTAVIPAANIMTTANELSRFFEIMRHGGELDGVRVLEPETIATALTE
                     QSHLELDLSLGFPTRFGYGLMLGAQLVSLFGRDTQRAFGHLGFTNTLGWADPQRGTSV
                     GIVTSGKPTVYPEVWRFLGLSQRITGETG"
     gene            complement(2667..3269)
                     /locus_tag="VFY44_11445"
     CDS             complement(2667..3269)
                     /locus_tag="VFY44_11445"
                     /inference="COORDINATES: protein motif:HMM:NF044451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5911100.1"
                     /translation="MNVNRRTQVDRSAATRTALIDAARPLFAEHGYGGVGTETIVRAA
                     GVTRGALYHQFADKTELFAAVFEAVEEDIGARIGEVVAATGATDPLEAMRTGAGAWLD
                     ACAEPEVQQIVLLDAPAVLGWERWREIGMRHGMGLVEAVLGQAMETGSVRRQPLRPLA
                     HVVVGALDEAALYVARAEDPVTAGREARAVVDQLIAGFAA"
     gene            3327..>4104
                     /locus_tag="VFY44_11450"
     CDS             3327..>4104
                     /locus_tag="VFY44_11450"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5911101.1"
                     /translation="MPTIDLPNGTVNYRVAGPEDSAAPPVVFVHGFLVDGTLWSDTMA
                     GLAERGVRSYAPDWPLGSHPIALDQGADQSPRGVARHIVSFLEAMELTDVTLVGNDTG
                     GAICQFVIDTDPARVGRLVLTNCDAFDSFPPAPFDVLFKSFRKAGAIRALMAPMRATP
                     ARHSPAGYGMLAENLDADQTRAWVEPCLADAGVRRDTAAFVRAVDPGELLDIAARLGS
                     FDRPVLLVWGDADRFFKIDFARRLAQIFPDARLVEVAGGKT"
BASE COUNT          517 a         1536 c         1514 g          537 t
ORIGIN      
        1 gccgcggacg atcgtgccac cggaggccac ggccaaggtg accatgcgcc tggcgccggg
       61 ccaggacccg gcggtcatga gcgaggtgct gctgggcctc ctgcgcgagg gcctgcccga
      121 gggcgccgag ctggacacgc gcgtgcagac ggcgctgccc gccctgttcg aggtcgacca
      181 gccggcgatg cagctggccg cgcgggcgct cgagaaggcc tgcggccgcc cgccggcgtt
      241 cgtccgctcg ggcggcagca tcccgatcgt ggctgagatg gcggcgcagg ggtacccggt
      301 gatcgtcggc ggcttcggcc tgcccgacga cgccatccac gcgcccaacg agtcgtacgc
      361 cctgcgctcg ctggagtggg gcgaggcggc ggcccgcgag ctgttcgaag cgctggccga
      421 gctgccgcgc tgatggcctg gaccgagaac cccaacctgg acaccgcggc ggtgaccagc
      481 cccacgacgg ccgccgcggg cttccaccac acgctggacg gctatgcgcc gacgccggtg
      541 cgcgagctcg cggacggaac gctggtcaag gacgagtccg gccgcctcgg cctgccggcg
      601 ttcaagatcc tgggcgcctc ctgggcggtc gagcgcgcgc tggccggggc ccccgccacc
      661 accacgctgg tggccgccag cgcgggcaac cacggccgcg cggtggcgca cgaggcggcc
      721 cggcgcggcc tggagtgccg ggtcttcctg ccggcgcgcg ccctggaggc gcggcgcgag
      781 gcgatcgcgg gcgagggcgc cgcggtcgag gtcgtggacg gcagctacga ggagtgcgtg
      841 gcgctggcgc gggccgccgg cgagcgcggc gacacgctcg agatcgccga cgtcggggac
      901 tcgggccccg cgcgcgacgt gatcgacggc tacgccacgc tcttcgacga ggccgcccac
      961 caggccgact tcgacgtgct ggtggtgccg atcggggtcg gctccctggg cgcggcggcc
     1021 gcccgcttcg gtgcccgggc cggcatcccg gtggtgggcg cggagcccga ggccgccgcc
     1081 tgcctgacgg cgtccctggc cgccggcgag ccgacggccg tgcccacccc gggcaccacg
     1141 atggcggggc tggactgcgc cgaggtctcg gccgccgcct ggcccgacct gcgggcgggc
     1201 gtctccggca ccgtcacggt ctccgaccac gaggcggccg acgcccagcg cgagctcgag
     1261 gccgcgggga tgtacatcgg cgagagcggc gcggcgccgc tggcggcact gcgacaggga
     1321 gggctgggcc cggacggggc ccgggtgctc ctggtggcca ccgaaggccg caccggggcg
     1381 ccgcaccccg gatagcatcg gcgcgtgcca cgggtccccc gcctgccgct catccccgat
     1441 cccctgcgcc ggatccgcgt gccgcgcgac ctggaggcgg tcaccgccgt cggggaggag
     1501 gccgaccccg ccgacgccgg catgacgcgc gactcggtcg agcagatctg gagcgatgcc
     1561 gtgacctggt accgctccgg ggtgcacccg gcgatgcagg tgtgcgtgcg gcgccacggc
     1621 acggtggtgc tggaccgggc catcgggcac gcgcgcggca acggccccgg cgacgcgccc
     1681 gacgagccca aggtccccgt ggccaccgac acgcccttct gcgtgtactc ggcctcgaag
     1741 gccatcaccg cgttcgtggt gcacgggctg atcgagcagg gcaaggtcgg cctggacgag
     1801 ccggtggccg agtacatccc gggctacggc atcaaccgca agcacgcgat cacggtgggg
     1861 cacgtgctgg cccatcgcgc cggcgtgccc aacatgcccg gccactcgtt cgacctggac
     1921 aagatgggcg accgcgagtt catccgcggc gcgctggtgg acgccgagcc gttcgccaag
     1981 cccggccgga tgctggccta ccacgcggtc tcgggcggct acatcctcgg cgagatcgtc
     2041 caccaggtca cgggcaagga catccgcacc gtgctggccg agtcgatcct ggatccgctg
     2101 ggcttccgct ggaccaacta cggcgtggcc gagcaggacc tggccgcgct gggcaccgac
     2161 tacgtgaccg gcccgccgct gctgccgccc gcgtcgcagc tgctgacgcg ggcgctgggc
     2221 gtgccgctgg acaccatcgt cgagtggtcg cgcgacccgc gctttcgcac cgcggtcata
     2281 cccgcggcca acatcatgac caccgccaac gagctgtcgc ggttcttcga gatcatgcgc
     2341 cacggtggcg agctggacgg cgtccgcgtg ctggagcccg agaccatcgc gaccgccctg
     2401 accgagcagt cgcacctgga gctggacctg tcgctgggct ttcccacccg cttcggctac
     2461 gggctgatgc tgggcgcgca gctggtcagc ctgttcggcc gcgacaccca gcgcgcgttc
     2521 ggccacctgg gcttcaccaa cacgctcggc tgggccgacc cgcagcgtgg gacgtcggtg
     2581 gggatcgtga ccagcggcaa gcccaccgtc tatcccgagg tctggcgctt cctcgggttg
     2641 tcgcagcgga tcacgggcga gacgggttag gcggcgaacc cggcgatcag ctggtcgacc
     2701 accgcgcgcg cctcgcgccc ggccgtgacc gggtcctcgg cgcgggccac gtacaacgct
     2761 gcctcgtcca gcgcgccgac caccacgtgc gccagcgggc gcagcggctg gcggcgcacg
     2821 ctgcccgtct ccatcgcctg ccccagcacc gcctcgacca ggcccatccc gtggcgcatg
     2881 ccgatctcgc gccagcgctc ccagcccagc acggcggggg cgtccagcag cacgatctgc
     2941 tgcacctcgg gctcggcgca cgcgtccagc caggcgcccg cgcccgtgcg catcgcctcc
     3001 agcgggtcgg tggcgccggt ggcggccacg acctcgccga tgcgcgcgcc gatgtcctcc
     3061 tcgaccgctt cgaacaccgc ggcgaacagc tcggtcttgt cggcgaactg gtggtacagc
     3121 gccccgcgcg tcacgccggc cgcgcgcacg atcgtctccg tgcccacccc gccgtagccg
     3181 tgttcggcga acaggggccg ggcagcgtcg atcagcgccg tgcgcgtggc ggcggagcga
     3241 tcgacctggg ttcggcggtt gacgttcata cagacagtat gtatcttgcg tgcagcctgt
     3301 acgcaaccta ctgcgaggag ccgacgatgc cgaccatcga cctgccgaac gggaccgtga
     3361 actaccgcgt cgccgggccc gaggactcgg ccgccccgcc cgtggtcttc gtgcacggct
     3421 tcctggtgga cggcacgttg tggagcgaca ccatggccgg cctggccgag cggggcgtcc
     3481 gctcctacgc gccggactgg ccgctgggct cgcacccgat cgcgctcgac cagggtgccg
     3541 accagtcgcc gcgcggggtg gcccggcaca tcgtgtcgtt cctcgaggcc atggagctca
     3601 ccgacgtgac gctggtgggc aacgacaccg gtggcgcgat ctgccagttc gttatcgaca
     3661 ccgacccggc ccgggtcggg cgcctggtgc tgaccaactg cgacgccttc gacagcttcc
     3721 cgccggcgcc gttcgacgtg ctgttcaagt cgttccgaaa ggccggcgcg atccgggcgc
     3781 tgatggcgcc catgcgggcg acgcccgcgc gccactcccc ggcgggctac gggatgctgg
     3841 ccgagaacct ggacgcggat cagacgcgtg cctgggtcga gccctgcctg gccgacgcgg
     3901 gcgtgcgccg cgacaccgcc gcgttcgtgc gcgcggtcga tcccggcgag ctgctggaca
     3961 tcgccgcgcg gctggggtcg ttcgaccgcc cggtgctgct ggtgtggggt gacgccgacc
     4021 gcttcttcaa gatcgacttc gcccgccgcc tggcgcagat cttccccgac gcgcggctgg
     4081 tcgaggtcgc gggcgggaag acgt
//
LOCUS       DASUPW010000511         3274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_671680, whole genome shotgun sequence.
ACCESSION   DASUPW010000511 DASUPW010000000
VERSION     DASUPW010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671680"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..463)
                     /gene="ssrA"
                     /locus_tag="VFY44_11455"
     tmRNA           complement(94..463)
                     /gene="ssrA"
                     /locus_tag="VFY44_11455"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(490..2079)
                     /locus_tag="VFY44_11460"
     CDS             complement(490..2079)
                     /locus_tag="VFY44_11460"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5911102.1"
                     /translation="MANPASVVLSPSQLETLAALGEERTAEVGEVLFRVGDQRYPFIV
                     IVEGEAAIQDAAGNEIVRHGAAGFLGEMNLLSGQTVYLNAVVTQPLRYIAVDRDDLRP
                     LLFEDGPLSDLVLSAFIARHEALQTRQGVGIEVIGPRSSAETRRVVDFARRSRMPLNW
                     RDTEHGDDPEAAALIGSLAPEQLPLVRLPGGPELQRPSNGHISRALGIGLELEAREEV
                     DLVVVGAGPAGLGAAVYGASEGLDTLVVEGTVLGGQAGTSRRIENYLGFPAGISGTEL
                     TARAITQARKFGARTATPYRALALEPGDDRHVVRLEDDREVVARAVVLATGAEYRRRP
                     VDDLADYEGISVFYAAGPPEAQRCAAARVAVVGGGNSAAQAAVWLARGGALVTLLHRR
                     ADLSETMSDYLLTDLERYGVTVRDRSEIAELHGTHGELEGVTLTDGERLPFGFLFLFL
                     GAEPCTDWLGDCVARDQKGFVLTGAAAGADALLETSVPGIYAAGDVRSGSVKRCATAV
                     GEGAMVVQFIHGRIADVAAPR"
     gene            2156..>3274
                     /locus_tag="VFY44_11465"
     CDS             2156..>3274
                     /locus_tag="VFY44_11465"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX5911103.1"
                     /translation="MGLLTVSAIALTGAGCGKVKGVEEQNGATADRAEKAGKQAAADA
                     GPPVKLPPVNVALLTMNGKDESSNRLTTQLEEASTKELGWVPSPCDGKGSPMELETCG
                     TSVIDEGPLDVILSNGVDPKLIPRTLRKAQFKNIPVINIGASIPNRRDSGMITADFAP
                     DDRAMAQKVDDYMIRRVERQPKASRRIVVLKSTGPGAKVRFDQLEEDIKGTGIRIVGE
                     GEVDPKKPDEAKNTVTNLLEKHKDLKAVWVADGNSVEFAGKAVDKAFKDLSFPDRPLV
                     VGFNADPKAAEAIRDDEADAVADVAYDGSLWVALDRIAERIGRDEQLNSVSTDDYPLD
                     FMDIALVTKDNVPAKKKFREPKEDHVTFFTEKWKEEFGS"
BASE COUNT          537 a         1123 c         1177 g          437 t
ORIGIN      
        1 ctctgtgggc gtggctgccg cggacgcagg gagaagacaa gcctggtggc tgtcttcgac
       61 cgaggacaaa ggcaggcgcg cccacagggc gcgagatgga gacgccggga atcgaacccg
      121 ggtccgcgga agagtgaaag acagcttcta cgagcgtagc ctgccctctg atctcgtccc
      181 ccgctcgcct ggcaggccgg gttgtggggg accagcctct gtgaaaagtc ccccccacac
      241 cagaggcgcg tgtggggagc aagcccgttt ctgatcccgg acatctcccc cacgggccga
      301 ggggaggcca ggagcgtcgc tggctagctg ttgctaggca gcgagcgcga atgagtgatt
      361 ctcatccgca actatggttt tggcggttgt tttacgaggc ctaccgccga cctcggctcg
      421 caactgcccc cacggatctg ccacgtcgaa gcctgtcgtc cccgtagaag caccttcaag
      481 gatagaccgt cagcgcgggg ctgccacatc ggcgatccgg ccgtggatga actggacgac
      541 catggcgcct tcgccgacgg cggtggcgca gcgcttgacc gagcccgagc gcacgtcccc
      601 ggcggcgtag atgccgggga cgctggtctc aagcagcgcg tcggcgcccg cggccgcccc
      661 ggtgaggacg aagcccttct ggtcgcgggc cacgcagtcg cccagccagt cggtgcacgg
      721 ctcggcgccc aggaagagga acaggaagcc gaacggcagc cgctcgccgt cggtgagggt
      781 gacgccctcc aactcaccgt gggtgccgtg cagctcggcg atctcgctgc ggtcgcgcac
      841 ggtgacgccg taccgctcga ggtcggtcag caggtagtcg gacatcgtct cgctcaggtc
      901 cgcgcggcgg tgcagcagcg tgaccagggc cccgccccgc gccagccaca cggcggcctg
      961 ggccgccgag ttgccgcctc cgaccacggc cacgcgcgcg gcggcgcagc gctgggcctc
     1021 cggggggccg gcggcgtaga acacgctgat gccctcgtag tcggccaggt cgtcgacggg
     1081 gcggcggcgg tactcggcgc cggtggccag caccacggca cgggccacga cctcgcggtc
     1141 gtcctcaagc cggaccacgt gccggtcgtc gccgggctcc agcgccagcg cccggtaggg
     1201 ggtcgccgtg cgggcgccga acttgcgtgc ctgcgtgatg gcgcgggccg tcagctccgt
     1261 gccgctgatc ccggccggga agccgaggta gttctcgatc cgccgcgagg taccggcctg
     1321 tccgcccagc accgtgccct cgacgaccag ggtgtcgagg ccctcggagg cgccgtagac
     1381 cgcggcgccg aggccggccg ggcccgcgcc gacgaccacg aggtccacct cctcgcgcgc
     1441 ctcgagctcg aggccgatcc cgagagcgcg ggagatgtgg ccgttcgagg gtcgctgcag
     1501 ctcggggcca cccggcagcc gcacgagcgg cagctgctcg ggcgccagcg acccgatcag
     1561 ggccgcggcc tcggggtcgt cgccgtgttc ggtgtcgcgc cagttcagcg gcatccggct
     1621 gcggcgggcg aagtcgacca cccgccgggt ctcggccgag gaccggggcc cgatcacctc
     1681 gatgcccacc ccctggcggg tctggagggc ctcgtgacgg gcgatgaacg ccgacaggac
     1741 gaggtcggac agggggccgt cctcgaacag gagcggccgc aggtcgtcgc ggtccacggc
     1801 gatgtaccgc agcggctgcg tgacgaccgc gttcaggtag acggtctggc ccgacaggag
     1861 gttcatctcg cccaggaagc cggcggcgcc gtggcgcacg atctcgttgc cggcggcgtc
     1921 ctggatcgcg gcctcgccct cgacgatgac gatgaacggg tagcgctggt cgccgacgcg
     1981 gaagagcacc tcgccgacct cggccgtccg ctcctccccc agagccgcca gcgtctccag
     2041 ctgtgacggg gacagcacca ccgatgcggg attcgccatc gatccagcgt acttgggccc
     2101 acctggcttg agctccggcg ctcctgggta acagcgccgc atgatgcgga gggcagtggg
     2161 gcttctgacg gtttctgcga tcgcgctcac gggcgccgga tgcggcaagg tcaagggggt
     2221 ggaggagcag aacggcgcca cggccgaccg ggcggagaag gccggcaagc aggccgccgc
     2281 cgacgccggg cccccggtca agttgcctcc cgtgaacgtc gccctgctga cgatgaacgg
     2341 caaggacgag tcctcgaacc ggctcacgac ccagctcgag gaggccagca cgaaggagct
     2401 cggctgggtg ccctcgccgt gcgacggcaa gggctcgccc atggagctcg agacgtgcgg
     2461 gaccagcgtg atcgacgagg gaccgctgga cgtgatcctc tcgaacggcg tggacccgaa
     2521 gctcatcccg cggacgctcc gcaaggcgca gttcaagaac atcccggtga tcaacatcgg
     2581 cgcgtcgatc cccaaccgcc gcgacagcgg gatgatcacc gccgacttcg ccccggacga
     2641 ccgggcgatg gcgcagaagg tcgacgacta catgatccgc cgcgtcgagc ggcagccgaa
     2701 ggcgtcccgc aggatcgtgg tgctgaagtc caccgggccg ggcgcgaagg tccgcttcga
     2761 ccagctcgag gaggacatca agggcaccgg gatcaggatc gtcggtgagg gcgaggtcga
     2821 cccgaagaag cccgacgagg ccaagaacac ggtcaccaac ctcttggaga agcacaagga
     2881 cctgaaggca gtgtgggtgg ccgatggcaa ctcggtggag ttcgccggca aggccgtcga
     2941 caaggcgttc aaggacctct cgttccccga tcgcccgctc gtggtcggct tcaacgccga
     3001 ccccaaggcc gccgaggcca tccgcgacga cgaggccgac gcggttgccg atgtggccta
     3061 cgacggctcg ctctgggtcg ccttggaccg gatcgccgag cggatcgggc gtgacgagca
     3121 gctgaactcg gtgtccaccg acgactaccc gctggacttc atggacatcg cgctcgtgac
     3181 caaggacaac gtgccggcca agaagaagtt ccgcgagccg aaggaggacc acgtgacctt
     3241 cttcaccgag aagtggaaag aggagttcgg ctcg
//
LOCUS       DASUPW010000512         4827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1503442, whole genome shotgun sequence.
ACCESSION   DASUPW010000512 DASUPW010000000
VERSION     DASUPW010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1503442"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1352)
                     /gene="ccrA"
                     /locus_tag="VFY44_11470"
     CDS             complement(93..1352)
                     /gene="ccrA"
                     /locus_tag="VFY44_11470"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR01751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HEX5911104.1"
                     /translation="MASVDLTDVTTVSTEPGELPEKMTAWVIREERQGEPRDAFQLEE
                     IEVPQPGAFEVVVRVMAAGVNYNNVWAALGQPVGVWKYGDHPEYGHHIGGSDASGIIW
                     KVGEGVTRWSVGDEVIIHCNQASYEDVEVHGLDPMAAPSQQIWGYETTWGSFAQFTKV
                     QAQQLLPKPTNLTWEEAASYGLVYFTAYRMLMDQCELQAGHKVLVWGGAGGLGVFAIQ
                     LCAAAGAQCVAVVSSEEKGKLCEQLGATGWIDRNEYKGMMRKGGESPEEEKARFKESR
                     RFGKAVSEVLGDAPDIVFEHVGQATFPTSVLTVKPFGKVVICAGTTGFNLDFDVRYLW
                     MKQKQIIGSHFANAYECMKANELIEQGLIRPVLWRTMPYEGVGEAHQLMRDNQHLGKI
                     AILVGAEEEGLGRTVDGPGAIQAEVGA"
     gene            1373..2515
                     /locus_tag="VFY44_11475"
     CDS             1373..2515
                     /locus_tag="VFY44_11475"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5911105.1"
                     /translation="MRLSERAAVTLLFFLNGAIFSSFFARLPAIKADLGASDGELGVA
                     LFFATGGLVVAQPLAGAMAAKLGARWPSLIGLAVYALGLPVAAAAPSVAALAAVLFVM
                     GLANGVLDVAMNVEGVAVERRRGRRVLSSMHAAFSFGGMTGAGLGGLAAAAGLDAEVH
                     LAVVAGLMLVGGAVAATGLPAPRPDRSRARAFVRPDRRLALLGAVAFCVLLAEGSVTD
                     WSAVYLNEGTGASEGLAAAGLSVFSLTMAIGRLAGDRTAERFSAPAVIRAGTLLAATG
                     LGLGLAVAEPALGIAGYALMGIGLSACFPLVIAASARGSGDAEAASIAIVSGAGYVGL
                     TAGPATIGLLSDAVGLRQALLLVVALALVASALAGAARADASRAGS"
     gene            2525..3139
                     /locus_tag="VFY44_11480"
     CDS             2525..3139
                     /locus_tag="VFY44_11480"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEX5911106.1"
                     /translation="MAHTGAVPPDTGRRLGRVDSFSDGLFSIAATLLVLSIDVPDVPE
                     ADLNGALDSLLPSLLAYFISFAVVGLFWLRHHEFFGRLQASDSRLATLNLIFLSFIAL
                     LPVPTELLGKYDNSAAPVVIYAVFILVLSLLLRALFHHARTAGLLAPGQVLPVDNVAA
                     AAVWGAFALSIPVAFVSPDLGMYCWILAWPMRFVFARVSGDSGP"
     gene            3136..4008
                     /locus_tag="VFY44_11485"
     CDS             3136..4008
                     /locus_tag="VFY44_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEX5911107.1"
                     /translation="MSARCARLADSTAFNLAIFAVILANAVVLGLETYDSVVEDAGGL
                     LDALNAAFLAVFVVELAIRIAAHGSRPQDFFRDGWNVFDFVIVAVALAPIGGSNSALL
                     RLARLARVLRVVRILPELRLLVVAVGRSLPGVASLAVLGILVLFVYGMVGWLAFGDQF
                     PDDWGNIGDAMLTLFVLLSLESLPDMIDQGLEVSQWSLVYYVSFVLVASFLLLNILIG
                     VVIDSLDKAREMEFTRAQADRRAAAAGTEGEGDDAAVELLQHVHELRRTLENVEREAR
                     AVVRAQEHGRSVRT"
     gene            4032..>4827
                     /locus_tag="VFY44_11490"
     CDS             4032..>4827
                     /locus_tag="VFY44_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911108.1"
                     /translation="MEAAFPLHQHRQDHVTADGDRVRIDGLEIHDETAARLVTESSEP
                     DSVIADAIEIGARVLDREQTAANAEFVKTEFEKVSKNVEAEFGDRAKVVAEELGRKVD
                     EVFDPESGHLSKSLEQLFSDGSSTAVQHKVKQIVTEAMQKSREDLRRQFSSADGADNP
                     LADFKDATVRELRRSSSEQQTSQQRLMERMGELEKQLQGLRDEKQKLEELAEERERGT
                     AKGRTYEEAVFAAVDAIAATGEDCAEPTGDVTGAGGRKGDVVVKIEG"
BASE COUNT          593 a         1660 c         1787 g          787 t
ORIGIN      
        1 ctcggcgacc gggcgccagt gcgcctcgaa cttgtcgccg cggaacggcg tgacgatcca
       61 gggcacctcg gccacgatcc cgctgctgtt gctcatgctc cgacctccgc ttggatggct
      121 ccggggccgt ccaccgtccg gcccagtccc tcttcctcgg ctccgaccag gatggcgatc
      181 ttgcccaggt gctggttgtc gcgcatcagc tggtgcgcct cgccgacgcc ctcgtacggc
      241 atcgtgcgcc acagcaccgg ccggatgagg ccctgctcga tgagctcgtt ggccttcatg
      301 cactcgtagg cgttggcgaa gtgcgagccg atgatctgct tctgcttcat ccacaggtag
      361 cggacgtcga agtccaggtt gaagcccgtg gtgccggcgc agatcacgac cttgccgaac
      421 ggcttcacgg tgagaacgga cgtggggaag gtggcctggc cgacgtgctc gaagacgatg
      481 tcgggcgcgt cgcccaggac ctcgctcacg gccttgccga accggcgcga ctccttgaag
      541 cgggccttct cctcctcggg cgactcgccg cccttgcgca tcatgccctt gtactcgttg
      601 cggtcgatcc agccggtggc gcccagctgc tcgcacagct tgcccttctc ctcgctggag
      661 accacggcca cgcactgggc gccggccgcg gcgcacagct ggatggcgaa gacgcccagg
      721 ccgccggcgc cgccccagac caggaccttg tggccggcct ggagctcgca ctggtccatg
      781 agcatccggt aggcggtgaa gtagacgagg ccgtaggagg cggcctcctc ccaggtcagg
      841 ttggtcggct tgggcaggag ctgctgggcc tggaccttgg tgaactgggc aaagctgccc
      901 caggtggtct cgtagcccca gatctgctgg ctcggcgcgg ccatcgggtc cagcccgtgc
      961 acctcgacgt cctcgtagga cgcctggttg cagtggatga tgacctcgtc gccgacgctc
     1021 cagcgggtga cgccctcgcc gaccttccag atgatccccg acgcgtccga gccgccgatg
     1081 tggtggccgt actcggggtg gtcgccgtac ttccacacgc cgacgggctg gccgagggcc
     1141 gcccagacgt tgttgtagtt cacgcccgcg gccatcacgc gcacgacgac ctcgaaggcg
     1201 cccggctggg gcacctcgat ctcctcgagc tggaaggcgt cgcggggctc gccctggcgc
     1261 tcttcgcgga tgacccaggc ggtcatcttc tcgggcagct cgccgggctc ggtgcttacg
     1321 gtggtgacgt cggtcaagtc aacggaggcc atggggcggg catggtactg gcatgaggtt
     1381 gtccgagcgc gccgccgtga ccctcctgtt cttcctgaac ggggcgatct tcagctcgtt
     1441 cttcgcccgg ctcccggcga tcaaggccga cctgggggcc agcgacggcg agctgggggt
     1501 ggccctgttc ttcgccaccg gcgggctggt ggtggcccag ccgctggccg gggcgatggc
     1561 ggccaagctc ggcgcccgct ggccgagcct gatcgggctg gccgtctacg cgctggggct
     1621 gccggtggcc gccgcggcgc cctcggtggc cgcgctggcc gccgtgctgt tcgtgatggg
     1681 cctggccaac ggcgtgctgg acgtggccat gaacgtggag ggcgtggccg tcgagcggcg
     1741 ccgcggccgc cgggtgctga gctcgatgca cgccgcgttc tcgttcggcg ggatgaccgg
     1801 ggccgggctt ggcggcctgg ccgccgctgc cgggctggac gccgaggtgc acctggccgt
     1861 ggtggcgggg ctgatgctgg tgggcggggc ggtggcggcc acgggcctgc cggcgccgcg
     1921 gcccgaccgc agcagggcgc gggcgttcgt gcgccccgac cgccgcctgg ccctgctggg
     1981 cgcggtggcc ttctgcgtgc tgctggccga gggctcggtg accgactgga gcgcggtcta
     2041 cctgaacgag ggcaccggag cctccgaggg gctggccgcg gccgggctgt cggtgttctc
     2101 gctgacgatg gccatcggcc ggctggccgg ggaccgcacg gcggagcgct tcagcgcacc
     2161 ggcggtcatc cgggcgggca cgctgctggc cgcgacgggg ctcgggctgg ggctggccgt
     2221 ggccgagccg gccctgggga tcgccggcta cgcgctgatg gggatcgggc tgtcggcctg
     2281 cttcccgctg gtgatcgcgg catcggcgcg gggctcgggc gacgcggagg cggcgtcgat
     2341 cgcgatcgtc tccggcgcgg gctacgtggg cctgacggcc ggcccggcca ccatcgggct
     2401 gctgtccgac gcggtcggcc tgcgccaggc gctgctgctg gtcgtggcgc tggcgctggt
     2461 ggcctcggcg ctggccggcg cggcccgcgc cgatgccagc cgggccggtt cctagcctgg
     2521 gcgcgtggca cacactggag ccgttccgcc cgacaccggc cgccggctcg gtcgcgtcga
     2581 ctcgttctcc gacgggctgt tctcgatcgc ggccacgctg ctggtgctgt cgatcgacgt
     2641 gcccgacgtg cccgaggccg acctcaacgg ggcgctggac agcctgctcc cgtcgctgct
     2701 ggcctacttc atctcgttcg ccgtggtggg cctgttctgg ctccgccacc acgagttctt
     2761 cggccgccta caggcctcgg actcgcggct ggcgaccctg aacctgatct tcctgtcgtt
     2821 catcgccctg ctcccggtcc cgaccgagct gctgggcaag tacgacaaca gcgcggcgcc
     2881 ggtggtcatc tacgcggtgt tcatcctggt cctgtcgctg ttgctgcggg cgctgttcca
     2941 ccacgcccgc acggcgggcc tgctcgcccc cgggcaggtg ctcccggtgg acaacgtggc
     3001 cgcggccgcg gtctggggcg cgttcgccct gtcgatcccg gtggcgttcg tctcgccgga
     3061 cctgggcatg tactgctgga tcctggcctg gccgatgcgg ttcgtgttcg cgcgggtgtc
     3121 cggggactcc ggcccgtgag cgcccgctgc gcccgcctgg cggactcgac ggcgttcaac
     3181 ctggccatct tcgccgtgat cctggcgaac gcggtggtgc tggggctgga gacctacgac
     3241 tcggtcgtcg aggacgccgg cggcctgctg gacgcgctca acgccgcgtt cctggccgtg
     3301 ttcgtggtcg agctggccat ccggatcgcc gcccacgggt cgcggccgca ggacttcttc
     3361 cgcgacggct ggaacgtgtt cgacttcgtg atcgtcgcgg tcgcgctcgc gcccatcggc
     3421 ggcagcaact ccgcgctgct gcgcctggcc cggctggcgc gcgtgctgcg cgtggtgcgg
     3481 atcctgccgg agctgcggct gctggtggtg gccgtggggc ggagcctgcc cggggtggcc
     3541 agcctggccg tgctggggat cctcgtgctg ttcgtctacg gcatggtcgg ctggctggcc
     3601 ttcggggacc agttcccgga cgactggggc aacatcggcg acgcgatgct gacgctgttc
     3661 gtgctgctct cgctggagag cctgccggac atgatcgacc agggcctcga ggtctcgcag
     3721 tggtcgctcg tctactacgt ctcgttcgtg ctggtggcgt cgttcctgct gctgaacatc
     3781 ctgatcggcg tggtcatcga ctcgctggac aaggcgcgcg agatggagtt cacccgcgcc
     3841 caggccgacc gccgggccgc cgccgcgggc accgagggcg agggcgacga cgccgcggtg
     3901 gagctgctgc agcacgtgca cgagctgcgc aggacgctcg agaacgtcga gcgcgaggcc
     3961 cgggcggtgg tgcgcgcgca agaacacggc cgttctgtca ggacctaggc gtagcgtgcg
     4021 acccatagtt catggaagcc gcattccccc tacatcagca tcgccaggac cacgtgaccg
     4081 ccgatggcga tcgcgtgcgc atcgatggcc tggagatcca cgacgagacc gccgcgcggc
     4141 tggtgaccga gtcatccgag cccgacagcg tcatcgcgga cgccatcgag atcggcgcac
     4201 gggtgctcga tcgtgagcag acggccgcca acgccgagtt cgtaaagacc gagttcgaga
     4261 aggtctcgaa gaacgtcgag gccgagttcg gggaccgcgc caaggtggtc gccgaggagc
     4321 tcggccgcaa ggtcgacgag gtcttcgacc ccgagtcggg gcacctgtcc aagtcgctgg
     4381 agcagctgtt ctccgacggc agctcgaccg cggtgcagca caaggtcaag cagatcgtca
     4441 ccgaggccat gcagaagtcg cgcgaggacc tgcgccgcca gttctcgtcc gccgacggcg
     4501 ccgacaaccc gctggccgac ttcaaggacg ccaccgtgcg cgagctgcgg cgaagctcct
     4561 cggagcagca gacctcacag cagcggctga tggagcgcat gggcgagctc gagaagcagc
     4621 tccagggcct gcgcgacgag aagcagaagc tcgaggagct ggccgaggag cgcgagcgcg
     4681 gcaccgccaa ggggcgcacc tacgaggagg ccgtgttcgc cgcagtcgac gcgatcgccg
     4741 cgacgggcga ggactgcgcc gagccgaccg gcgacgtgac cggcgcgggc gggcgcaagg
     4801 gcgacgtcgt ggtcaagatc gagggcg
//
LOCUS       DASUPW010000513         2886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_535089, whole genome shotgun sequence.
ACCESSION   DASUPW010000513 DASUPW010000000
VERSION     DASUPW010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535089"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VFY44_11495"
     CDS             complement(<1..369)
                     /locus_tag="VFY44_11495"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX5911109.1"
                     /translation="MALDPLPDPLYLLELRSTRVAAAELLLLAVAGGTLGAFVVLRRL
                     AFYTHAVGTATFPGVVVAEAARFSPRLAALAVAVAYAGGVERAGRAGRDPGGAATGLI
                     LVLALAGGTILASDVFESGAG"
     gene            complement(400..1110)
                     /locus_tag="VFY44_11500"
     CDS             complement(400..1110)
                     /locus_tag="VFY44_11500"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5911110.1"
                     /translation="MKHHEPPAPPELGEHDGLSYALFLPERPAAGGVVILHGAGSRKE
                     SHFDFGRVCRDRGLAALAFDARGHGSSGGRFGPAAVGDVLAMRDVLSAHAPRIALRGS
                     SMGGFLAIQAAAAEPSIAAVAAICPAPPELLARGIASGDLVDFEVDADATVEWLGTLD
                     LAASAARLSPRTALLLLHAQGDERVPYAASQELHDAAGEPKRLIVFPGGHHRSLQHDL
                     EVQNLTLRFLKDAMRPPV"
     gene            1124..2404
                     /gene="lysA"
                     /locus_tag="VFY44_11505"
     CDS             1124..2404
                     /gene="lysA"
                     /locus_tag="VFY44_11505"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX5911111.1"
                     /translation="MSTDRTVADHGSALPSGLERVYPMGSDLGPGGALRIAGCDAREL
                     AREFGTPAYVYAEDDLRTRARATRDAFAARTDRFEVLYASKAFPATAALRIFAEEGLS
                     VDCASGGEMHLALNAGFDPERIYLHGNNKSEAELELAVESGVGHVIVDSFDEIERLER
                     VAAGSGQRILMRVTPGIEPETHKAIRTGQEDSKFGIPMRDVERALERIGRSGLELRGL
                     HAHIGSQVFDLDVYESLADVLRQIGDFPLLNLGGGFAIAYTREDRPPPAEEYAEAMLA
                     HAPEGVTVLCEPGRSLVGNAGVSLYTVGTVKEVPGVRTYVAVDGGMADNIRPMLYGAV
                     YEADVADRFGDSTPCRIVGMHCESGDILISEGALEDPRPGDVVVVPATGAYGHAMASN
                     YNAVPRPPVVFCKDGDARLVVRRETYDDLTRRDV"
     gene            2397..>2886
                     /locus_tag="VFY44_11510"
     CDS             2397..>2886
                     /locus_tag="VFY44_11510"
                     /inference="COORDINATES: protein motif:HMM:NF015412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911112.1"
                     /translation="MSEASPFRIGLLGYGTVGKAFEVLVGERTEAIAAEVGFRPEISG
                     VLTTSRGDFDDILERSDLIVELIGGTEPALDYALRAMEAGKHFVTANKQLVSRHGEQL
                     FEVAHRKGVQLRFEAAVGGVVPAIRVISETLAAAHIERVHGIVNGTTNFILTAMERTG
                     ASY"
BASE COUNT          408 a         1044 c         1078 g          356 t
ORIGIN      
        1 acccgccccg gactcgaaca cgtcgctggc caggatcgtc cccccggcca gggccaggac
       61 caggatcagc cccgtggccg cgccgccggg gtcgcgcccg gcgcggccgg cccgctcgac
      121 cccgccggcg taggccacgg cgacggcgag cgcggccagg cggggtgaga agcgggcggc
      181 ctcggccacc accacgcccg ggaacgtcgc cgtgcccacg gcgtgggtgt agaaggccag
      241 ccgccgcagc accacgaagg cgccgagggt gccgccggcc acggccagca gcagcagctc
      301 ggcggcggcg acccgcgtcg agcggagctc gagcaggtac agggggtccg ggagcgggtc
      361 cagtgccacc gccccagtct aaatgagagc gattctcgac tacaccggcg gccgcatcgc
      421 gtccttcagg aagcgcagcg tcaggttctg gacctccagg tcgtgctgga gcgagcggtg
      481 gtgcccgccc gggaacacga tcagccgctt gggctcgccg gccgcgtcgt gcagctcctg
      541 gctggccgcg tagggcacgc gctcgtcgcc ctgggcgtgc agcagcaaca gcgcggtgcg
      601 tggcgagagc cgggcggcgg acgccgcgag gtccagcgtg cccagccatt ccacggtcgc
      661 gtcggcgtcg acctcgaagt ccaccaggtc cccggaggcg atcccgcgcg ccagcagctc
      721 cggcggcgcg gggcagatcg cggccaccgc ggcgatcgac ggctcggccg ccgcggcctg
      781 gatggccagg aagccgccca tgctggagcc gcgcagggcg atccgtggcg catgggcgga
      841 gagcacgtcg cgcatggcca gcacgtcgcc caccgcggca ggcccgaagc gcccgcccga
      901 gctgccgtgg ccgcgggcgt cgaaggccag agcggccagg ccgcggtcac ggcagacgcg
      961 gccgaagtcg aagtggctct ccttgcgcga ccccgccccg tgcaggatca ccacgcctcc
     1021 ggctgcggga cgctccggca ggaacagcgc gtaggagagc ccgtcgtgct cgccgagctc
     1081 gggcggtgcg ggcggctcgt ggtgcttcat actcgcggac cctatgagca ccgaccgcac
     1141 cgtggccgac cacgggagcg cgctgccctc cgggctggag cgcgtgtacc cgatgggctc
     1201 cgacctgggg cccggcggcg ccctgcgcat cgccggctgt gacgcgcgcg agctggcgcg
     1261 cgagttcggc acgcccgcgt acgtctacgc ggaggacgac ctgcgcaccc gggcccgcgc
     1321 gacccgcgac gcgttcgccg cccgcaccga ccggttcgag gtgctgtacg cgtccaaggc
     1381 cttccccgcc accgcggcgc tgcggatctt cgccgaggag gggctgtcgg tggactgcgc
     1441 gtccggcggc gagatgcacc tggcgctgaa cgcgggcttc gaccccgagc ggatctacct
     1501 gcacgggaac aacaagtccg aggccgagct ggagctggcg gtcgagtcgg gcgtgggcca
     1561 cgtgatcgtg gacagcttcg acgagatcga gcggctggag cgcgtcgcgg ccgggtccgg
     1621 acagcggatc ctgatgcgcg tgacgcccgg catcgagccc gagacccaca aggccatccg
     1681 caccggccag gaggactcca agttcggcat ccccatgcgc gacgtggagc gggcgctcga
     1741 gcgcatcggc cgctccggcc tggagctgcg cgggctgcac gcccacatcg gctcgcaggt
     1801 cttcgacctg gacgtgtacg agtcgctggc cgacgtgctg cgccagatcg gcgacttccc
     1861 gctgctgaac ctgggcggcg gcttcgccat cgcctacacc cgtgaggacc gcccgccgcc
     1921 ggccgaggag tacgccgagg ccatgctggc gcacgcgccc gagggcgtga ccgtgctgtg
     1981 cgagcccggg cgctcgctgg tgggcaacgc cggcgtgtcg ctgtacacgg tgggcacggt
     2041 caaggaggtc ccgggcgtgc gcacgtacgt ggccgtggac ggcgggatgg ccgacaacat
     2101 caggccgatg ctctacggcg ccgtgtacga ggccgacgtg gccgaccgct tcggcgattc
     2161 caccccctgc cggatcgtgg ggatgcactg cgagtcgggc gacatcctga tcagcgaggg
     2221 cgcgctggag gacccgcgcc cgggcgacgt cgtggtcgtg ccggccaccg gcgcgtacgg
     2281 tcacgcgatg gccagcaact acaacgcggt cccgcggccg ccggtcgtgt tctgcaagga
     2341 cggcgacgcc cgcctggtgg tccggcgcga gacctacgac gatctgaccc gccgcgatgt
     2401 ctgaggccag tccgttccgg atcggcctgc tgggctacgg caccgtcggc aaggcgttcg
     2461 aggtgctggt gggcgagcgc accgaggcga tcgccgccga ggtgggcttc cggcccgaga
     2521 tctccggggt cctgaccacc agccggggcg acttcgacga catcctcgag cgctccgacc
     2581 tgatcgtgga gctgatcggc ggcacagagc cggcactgga ctacgcgctg cgcgcgatgg
     2641 aggcgggcaa gcacttcgtg accgcgaaca agcagttggt ctcgcgtcac ggcgagcagc
     2701 tgttcgaggt cgcgcaccgc aagggcgtgc agctgcggtt cgaggccgcg gtgggcggcg
     2761 tggtcccggc catccgcgtg atctccgaga ccctggccgc cgcccacatc gagcgcgtgc
     2821 acggcatcgt caacgggacg accaacttca tcctcaccgc catggagcgc accggcgcct
     2881 cctacg
//
LOCUS       DASUPW010000514         2693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_397535, whole genome shotgun sequence.
ACCESSION   DASUPW010000514 DASUPW010000000
VERSION     DASUPW010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397535"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VFY44_11515"
     CDS             complement(<1..361)
                     /locus_tag="VFY44_11515"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX5911113.1"
                     /translation="MADAPVKKRGFKQLSGMDRSDGPPPETRKGQPLRPWTIPNLISY
                     VRLALLPVFVVIALNTDDGRSFATAALYFGIAWGDQLDGLAARLTGQYSRLGALLDPL
                     TDRALVLAGAIVCWNFEL"
     gene            complement(343..2043)
                     /locus_tag="VFY44_11520"
     CDS             complement(343..2043)
                     /locus_tag="VFY44_11520"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX5911114.1"
                     /translation="MTRLTRYLLPTEKEAPADAEALSHKLMVRAGLIRQLGAGMWTWM
                     PAGLRSHRKAEQIIREEMDAIGCLEMLMPVIAPAELWQKSGRYGIDELFKLQDRRGAE
                     MVLAMTHEEAITFHMARAIRSYRDLPLMAYHIQTKERDEPRPRAGVLRTREFIMKDAY
                     SFDRDQEGLAAAYDLQAGAYDRIFDRAGLEWYRVESDVGAMGGIGAHEYMAPCAAGEN
                     DVALSDAGYAANVEIATATPKPVQGLPGPLEAPERVDTPGAKTVDQVCAALEVPAGAL
                     IKALPVLVGEEAEPLLVVVRGDHRLNEIKLANALGRPARPATAEEVGDVFGAPPGFIG
                     PVGATAPVLADEALRGLAGMVCGANEPDAHLRGVEVGRDFQCDFADVRTVEAGDTCPG
                     GAEIRIEPAIEVGNIFKLGTRYSEPLEATYLDVDGKEQLIWMGSYGIGPARIVAAAVE
                     QFGDDRGISWPRALAPFDIHLVTLGKEGEDARRVAEEIYEELQREGMDVIYDDRDASP
                     GEKFADAELLGCPLRLTVGRKGLENGEVEAQVRRGQAKQSLPLEGAARAAVELWRTLP
                     "
     gene            2123..>2693
                     /locus_tag="VFY44_11525"
     CDS             2123..>2693
                     /locus_tag="VFY44_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911115.1"
                     /translation="MTDLSPTARRSLNAVASALVLACAVFAAQALTGVGGASTDEIFL
                     NWVYDGILLGASLLCLARGLLVRRQRWVWLIMGAGLLAWTAGEIYYWLGAPDSGELPI
                     PSLADVGYLALYPAAAVAVVLLMRDQISDLRASLCLDGVTVALAVAALASALVATPIL
                     DATAGDDPLASLTNLAYPVADLMLLALVIA"
BASE COUNT          333 a          998 c          944 g          418 t
ORIGIN      
        1 gcagctcgaa gttccagcac acgatcgccc cggccaggac cagcgctcgg tcggtcaggg
       61 ggtccaggag ggctccgagg cggctgtact ggccggtcag gcgcgccgcc aggccgtcca
      121 gctggtcgcc ccaggcgatg ccgaagtaca gcgccgccgt ggcgaagctg cggccgtcgt
      181 cggtgttcag cgcgatcacc acgaacaccg gcagcagcgc caggcggacg taggagatga
      241 ggttggggat cgtccacggc cgcagcggct ggcccttgcg cgtctcgggc ggcggaccgt
      301 ccgagcggtc catccccgag agctgcttga agccgcgctt ctttacggga gcgtccgcca
      361 cagctccacg gcggcgcgcg ccgcgccctc cagcggcagc gactgcttcg cctggccgcg
      421 cctgacctgt gcctcgacct cgccgttctc caggcccttg cgccccacgg tcagccgcag
      481 cgggcagccc agcagctcgg cgtcggcgaa cttctcgccg gggctggcgt cacggtcgtc
      541 gtagatcacg tccattcctt cgcgctggag ctcctcgtag atctcctcgg ccacgcgacg
      601 ggcgtcctcg ccctccttgc ccagggtgac caggtggatg tcgaacggcg ccagcgcccg
      661 cggccaggag atcccgcggt cgtcgccgaa ctgctccacg gccgcggcga cgatccgggc
      721 agggccgatg ccgtaggaac ccatccagat cagctgctcc ttgccgtcca cgtccaggta
      781 ggtggcctcc agcggctcgg agtagcgcgt cccgagcttg aagatgttgc ccacctcgat
      841 cgccggctcg atgcggatct cggcgccgcc ggggcaggtg tcgccggcct cgaccgtgcg
      901 cacgtcggcg aagtcgcact gaaagtcacg gccgacctcc accccgcgca ggtgcgcgtc
      961 gggctcgttg gcgccgcaca ccatcccggc caggccgcgc agcgcctcgt cggccagcac
     1021 gggggcggtc gcgcccacgg ggccgatgaa gccgggtggc gcgccgaaca cgtcgcccac
     1081 ctcctccgcg gtcgccggcc gcgcgggccg gccgagcgcg ttggccagct tgatctcgtt
     1141 caggcggtgg tcgccgcgga ccacgaccag cagcggctcg gcctcctcgc cgaccagcac
     1201 gggaagggcc ttgatcagcg cgccggccgg cacctcgagc gcggcgcaca cctggtcgac
     1261 ggtcttggcc ccgggggtgt ccacgcgctc gggcgcctcc agcggcccgg gcaggccctg
     1321 caccggcttg ggcgtggccg tggcgatctc cacgttcgcg gcgtacccgg cgtccgagag
     1381 cgccacgtcg ttctcaccgg ccgcgcacgg cgccatgtac tcgtgtgccc cgatgccgcc
     1441 catggccccc acgtcggact cgacccggta ccactccagg ccggcgcggt cgaagatccg
     1501 gtcgtacgcg cccgcctgga ggtcgtaggc ggccgccagg ccctcctggt cgcggtcgaa
     1561 cgagtaggcg tccttcatga tgaactcgcg cgtgcgcagg acccccgcgc ggggccgcgg
     1621 ctcgtcccgt tccttggtct ggatgtggta cgccatcagc ggcaggtcgc ggtaggagcg
     1681 gatggcccgg gccatgtgga aggtgatggc ctcctcgtgg gtcatggcca gcaccatctc
     1741 ggcgccgcgg cggtcctgga gcttgaacag ctcgtcgatg ccgtagcggc cgctcttctg
     1801 ccacagctcc gccggggcga tcaccggcat cagcatctcc aggcagccga tggcgtccat
     1861 ctcctcgcgg atgatctgct cggccttgcg gtgcgaccgc aggcccgcgg gcatccacgt
     1921 ccacatgccc gccccgagct ggcggatcag ccccgcgcgg accatcagct tgtgcgagag
     1981 ggcctcggcg tcggccgggg cctccttctc ggtgggcaac agatagcggg tcagccgggt
     2041 catggccgcc ggagtctatg ggcggtgccc gccgtgacgg cttcagtacc ccatctggga
     2101 tgccgatgca acagatggtg ccatgacaga cctgtccccc acagcccgcc ggagcctgaa
     2161 cgcggtcgcg tccgcgttgg tgctcgcgtg cgcggtcttc gcggcgcagg cgctcaccgg
     2221 cgtcggcgga gccagcacag acgagatctt cctgaactgg gtctacgacg gcatcctgct
     2281 gggtgcctcg ctgctgtgtc tggcgcgcgg gcttctggtc cggcgccagc gctgggtgtg
     2341 gctgatcatg ggcgcaggcc tgctggcctg gacggccggc gagatctact actggctcgg
     2401 ggccccggat tccggcgagc tgccgatccc ctcgctcgcg gacgtgggct acctggccct
     2461 gtaccccgct gccgcggtcg ccgtggtcct gctcatgcgc gaccagatct cggatctgcg
     2521 cgccagcctg tgcctggacg gcgtgaccgt ggcgctggcc gtcgcggcgc tcgcgtccgc
     2581 gctcgtggcc acgccgatcc tggatgccac ggccggtgac gaccctctgg cctcgctcac
     2641 gaacctggcc tacccggttg ccgacctgat gctgctggcg ctcgtgatcg cgg
//
LOCUS       DASUPW010000515         2674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_672886, whole genome shotgun sequence.
ACCESSION   DASUPW010000515 DASUPW010000000
VERSION     DASUPW010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672886"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VFY44_11530"
     CDS             complement(<1..602)
                     /locus_tag="VFY44_11530"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX5911116.1"
                     /translation="MANADTLITGATGFIGGRLLDALAGEREVRALVRDASKLDDERA
                     DVAEGDLTDRDAVRRALEGVEVAYYLVHSMEPGDDDFASKDREMAEAFVAAAEAEGVQ
                     RLVYLGGVEPDGRTSAHLQSRLDVEKVLGSGEPELVALRASMVVGAESDSFRTLAQIV
                     GRIPVLALPPWRDNRTQPVAVADVIGALRAARDVEPGVYDV"
     gene            complement(649..891)
                     /locus_tag="VFY44_11535"
     CDS             complement(649..891)
                     /locus_tag="VFY44_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911117.1"
                     /translation="MPERTPDGRHVIIDGRRWRATDPSLPEARRRELVRELMSARSAV
                     GWATRRKDPTAERAARDRVHAAKVALGERGPKWWER"
     gene            913..1173
                     /locus_tag="VFY44_11540"
     CDS             913..1173
                     /locus_tag="VFY44_11540"
                     /inference="COORDINATES: protein motif:HMM:NF023056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3253 domain-containing protein"
                     /protein_id="HEX5911118.1"
                     /translation="MSGPTGEQIEQTVVELLEARDPGKTICPSDAARALAGQADFRPL
                     MEPVRARARQMVGRGDLEITQGGKVVDPDTARGAIRLRLPQG"
     gene            complement(1170..1907)
                     /locus_tag="VFY44_11545"
     CDS             complement(1170..1907)
                     /locus_tag="VFY44_11545"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5911119.1"
                     /translation="MSVAVVALSVLVGAALQSATGFGFALVTAPAVFAVFAPGEALTL
                     LVLLSVVLSLLVLFGERRGVDVRRGDVARMTAWGIPGMAAGVLVLRAVDKPVLQVGVG
                     LAVVAAAAIELRRVDDAVGGSPWPVEPVGVVAGLLTTTTGVNGPAMIMYYLRTGPDQH
                     QVRDCMAATFLAFTPFAVLALALAGELGLGEVDPAELAGLVALVVVGRPLGRMLFLRM
                     SAAAFRTAGIALALLAGLGSVIVGLAG"
     gene            1927..>2674
                     /locus_tag="VFY44_11550"
     CDS             1927..>2674
                     /locus_tag="VFY44_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911120.1"
                     /translation="MTRRRVLTTIAVTAVVLVGLYLLVPRIAGLEATWHRIEDGDPWW
                     LALGVALEAVSFAGYVWLLRAVVRRGGRSMSAAAGWRLTLAGVAATRIVTVGGAGGIA
                     VTVVGLRDEGFTTREASERQAVDLVLLYAVFFGLIVACGVALAALGEGHPAVTLAPAG
                     LSAVIIALALAVARVPQDFERRLRGRWGKRGAALAALPAVLADGVRGGWSMLRRGDPA
                     VVGALVWWLADIGVFVACVGAFGGHIDLLVL"
BASE COUNT          325 a         1037 c          987 g          325 t
ORIGIN      
        1 acgtcgtaga cgccgggctc cacgtcgcgc gccgcccgca gcgcgccgat cacgtcggcc
       61 acggcgaccg gctgggtccg gttgtcccgc cacggcggca gggccagcac cgggatccgc
      121 ccgacgatct gggccagcgt gcggaacgag tcgctctccg cgcccacgac catcgaggcc
      181 cgcagcgcca ccagctccgg ctcgcccgac cccaggacct tctccacgtc cagccgcgac
      241 tgcaggtggg cgctggtccg gccgtcgggc tcgacgccgc ccaggtagac caggcgctgg
      301 acgccctcgg cctcggccgc cgccacgaac gcctccgcca tctcgcggtc cttgctggcg
      361 aagtcgtcgt cgcccggctc catcgagtgc accaggtagt aggccacctc gacgccttcc
      421 agggcgcgcc tgacggcatc ccggtcggtc aggtcgccct cggccacgtc cgcccgctcg
      481 tcgtcgagct tggacgcgtc gcggaccagc gcccgcacct cgcgctcgcc cgccagggcg
      541 tccaggagcc ggccaccgat gaatccggtg gccccggtga tgagggtgtc cgcgttggcc
      601 atggctacgc ctacgcggcg cggcgcgcgc gggatgaggc cggcgtgctc agcgctccca
      661 ccacttcggc ccgcgctcgc ccagcgccac cttggccgcg tgcacccggt cgcgagcggc
      721 gcgctcggcc gtggggtcct tgcgccgcgt ggcccacccc accgccgacc gcgccgacat
      781 gagctcgcgg acgagctccc tgcgcctggc ctcgggcagc gacgggtccg tcgcccgcca
      841 gcggcgtccg tcgatgatca cgtggcggcc gtcgggcgtt cgctcgggca tgccctgggt
      901 acgtccgaac gcgtgagcgg gcccacggga gagcagatcg agcagaccgt cgtggagctg
      961 ctggaggccc gcgacccggg caagaccatc tgcccctccg acgccgcccg cgcgctggcc
     1021 ggccaggccg acttccggcc gctgatggag cccgtccggg cccgggcgcg ccagatggtg
     1081 ggccgcggcg acctggagat cacccagggc gggaaggtcg tggacccgga caccgcgcgc
     1141 ggggcgatcc ggctccgcct gccgcagggc tagccggcca ggcccacgat cacgctgccc
     1201 agcccggcca gcagggccag cgcgatgccg gccgtgcgga acgccgccgc gctcatccgc
     1261 aggaacagca tccgccccag cgggcggccg acgaccacga gggccacgag ccccgccagc
     1321 tcggccgggt ccacctcccc gagcccgagc tcgccggcca gggccagcgc caggaccgcg
     1381 aacggcgtga aggccaggaa ggtggcggcc atgcagtcgc gcacctggtg ctggtccggt
     1441 ccggtgcgca ggtagtacat gatcatcgcc ggcccgttga cgccggtcgt cgtggtcagc
     1501 aggccggcca ccacgccgac gggctcgacc ggccagggcg acccgccgac cgcgtcgtcc
     1561 acccgccgga gctcgatcgc cgcggccgcg accacggcga gccccacgcc cacctggagc
     1621 accggcttgt ccaccgcccg cagcacaagg accccggcgg ccatgcccgg gatcccccat
     1681 gcggtcatgc gcgccacgtc gccacgccgg acgtccacgc cgcgccgctc gccgaacagc
     1741 accagcaggc tgagcaccac ggacagcagc accaggaggg tcagcgcctc gcccggcgcg
     1801 aacacggcga acacggcggg cgcggtcacc agcgcgaagc cgaagcccgt cgcggactgc
     1861 agcgccgccc cgaccaggac gctcaaggcc acgaccgcga cactcacccc actagcctga
     1921 ccgaacgtga cccggcggcg cgtgctgacc accatcgccg tgaccgccgt ggtgctggtg
     1981 gggctctatc tgctggtgcc gaggatcgca gggctggagg cgacctggca ccggatcgag
     2041 gacggcgacc catggtggct ggccctgggc gtggcgctgg aggcggtctc gttcgccggc
     2101 tacgtgtggc tgctgcgcgc cgtcgtccgc cggggcggac gctcgatgtc cgcagccgcc
     2161 gggtggcggc tcacgctggc cggcgtggcg gccacgcgga tcgtcacggt gggcggtgcg
     2221 ggcgggatcg ccgtgaccgt ggtcggcctg cgggacgagg gcttcaccac ccgcgaggcg
     2281 tccgagcgcc aggcggtgga cctggtgctg ctctacgccg tcttcttcgg gctgatcgtg
     2341 gcctgcggcg tggcgctggc cgcgctgggc gagggccacc ccgccgtgac cctggctccc
     2401 gccgggttgt ccgcggtgat catcgcgctg gcgctggcgg tggcccgtgt gccccaggac
     2461 ttcgagcgca ggctgcgcgg ccggtggggc aagcgcggcg cggcgctggc cgcgcttccc
     2521 gcggtgctgg ccgacggcgt gcgcggcgga tggtcgatgc tgcgccgggg cgacccggca
     2581 gtggtgggcg cgctggtctg gtggctggcc gacatcgggg tcttcgtggc gtgcgtgggc
     2641 gcgttcggcg gccacatcga cctgctcgtg ctgg
//
LOCUS       DASUPW010000516         9586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_397868, whole genome shotgun sequence.
ACCESSION   DASUPW010000516 DASUPW010000000
VERSION     DASUPW010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9586
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397868"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="VFY44_11555"
     CDS             complement(<1..649)
                     /locus_tag="VFY44_11555"
                     /inference="COORDINATES: protein motif:HMM:NF014671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX5911121.1"
                     /translation="MPEAIDSGVARTWIERVAGEQRELVRDEVAIEEPLEIRVDARAL
                     AVTMRTPGADEELAVGFLLGEGLIAGREDVAEAGPNAELEANIVEVTTRAGLRRDPFA
                     ERRFHMTSSCGVCGKGALEQVRLEVPARPPAPVRIEPAQVLDLPGRARPAQDAFDRTG
                     GIHATALFTAAGELLVLREDVGRHNAMDKAIGALLLDRGEAAGALACLSGRIGFEL"
     gene            complement(714..1093)
                     /gene="rnpB"
                     /locus_tag="VFY44_11560"
     ncRNA           complement(714..1093)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFY44_11560"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1403..2047)
                     /gene="lexA"
                     /locus_tag="VFY44_11565"
     CDS             complement(1403..2047)
                     /gene="lexA"
                     /locus_tag="VFY44_11565"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX5911122.1"
                     /translation="MAELKDLNLTKRQQEIFDFIGRYSAKYGYPPTVRDIGKAIGLTS
                     SSTVHAHLANLEKAGLLRRDPTKPRAIELLVDKAKRVKTAVETAAGGALPVVGQVAAG
                     SPILAEENIEEYVQIPELAGGDKGEFILAITGDSMINAGIHDGDHVVVRSQETASDGE
                     IVVAMVGDAEATCKRFFRESDHIRLQPENDLLEPILSDEVQVLGRVVGVLRRVS"
     gene            2167..2250
                     /locus_tag="VFY44_11570"
     tRNA            2167..2250
                     /locus_tag="VFY44_11570"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2201..2203,aa:Leu,seq:gag)
     gene            2339..2686
                     /locus_tag="VFY44_11575"
     CDS             2339..2686
                     /locus_tag="VFY44_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911123.1"
                     /translation="MVERSGGVAGRFARMRVDPCGTVAVRRPPEDEVTLQLDERRLAH
                     LRSLVTGSHFRSLSATYFLPRPAVPGGYAWRISHAGTTVATRDGEAPRGLGALMHELA
                     ALIDDAVFAGGHR"
     gene            complement(3072..3296)
                     /gene="secG"
                     /locus_tag="VFY44_11580"
     CDS             complement(3072..3296)
                     /gene="secG"
                     /locus_tag="VFY44_11580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX5911124.1"
                     /translation="MEAILSGVQVVLSIVLIVLVLLHSGKDTGLSGAFGVGGGSGPLG
                     GGSLVERNLNRWTIAFATLFVVNVIVLLKI"
     gene            complement(3316..3603)
                     /locus_tag="VFY44_11585"
     CDS             complement(3316..3603)
                     /locus_tag="VFY44_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911125.1"
                     /translation="MEYGLALIFLVAVVTLIVVRPVVYREDDEADVGAARAELEAAKE
                     AKYREIRDAELDRQMGKLTAEDHRAIDRELRADAIDILRRLDELEGEAPRA"
     gene            complement(3615..5780)
                     /locus_tag="VFY44_11590"
     CDS             complement(3615..5780)
                     /locus_tag="VFY44_11590"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEX5911126.1"
                     /translation="MAGVGSACLIVALLTAVYAAGSSVYGAVSGRRDFVVSGRRAMYC
                     VAALCIGATAILQSAFLRSDFSFKLVADGSSTDTPTFYKVTAMWATQDGSLLLWALLL
                     SIFTSIVLFLTRRSLREIAPWATAVLGVVAAFFLLLMVGWENPFTTLANPPAEGVGLN
                     PLLRHPAMMIHPPMLYTGYVGFSIPFAFAVGALITRSTGADWIRATRRFALVAWTFLG
                     VGIMLGALWSYTELGWGGYWAWDPVENASLLPWLTGTAFLHSVMVQEKRGMLKVWNVS
                     LIIATFVLALLGTFLVRSGILESIHAFGASTIGTQFLTFIALVIVFSVALVVWRLPDL
                     RSTSRLDSLISREAFFLLNNLILVALALVVFWGTFFPLISEAVTGTEASVGPPWFNRV
                     VTPLALVLVALMTLGPMLAWRRVSAGGLWRTVRAPFAFAVLVLIVLALATNAPDSLTA
                     LAMFGLVALLLGVVVQEFARGALARRQLTGEPLHTAFGQLVGRNRRRYGGYIVHAGIA
                     VLFLGVAGSSAFHQQKDVRLSPGQSFSQGDYRVTYVRPTARLGADDAGTGAPISFGAV
                     MRVRKGDEVKTLHPSRNYFPTQDPTKGATARYFEGEATSEVDVRWGLRRDFWLAMRPD
                     ISSLDKPIRDADRKFPQPTADTQALLIGALAENYRRNPPPATFRAIVSPLVSWIWIGG
                     GIVLLGALVAAWPSPEAKLRRVRSLYSARLGKELAGRAS"
     gene            complement(5782..6189)
                     /locus_tag="VFY44_11595"
     CDS             complement(5782..6189)
                     /locus_tag="VFY44_11595"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX5911127.1"
                     /translation="MDPSRKRRARLVLALSVAVLLAGVLVYTSFSASTEASTPSEVMK
                     NAEPGRSYELTGKVVNDSVKRVGDRLEFAIRDRDGTASVPVSYAGVVPDPFREGREVI
                     VSGKLEGRRFAAERDSLVTKCPSKFTEDKKKQT"
     gene            6294..8264
                     /locus_tag="VFY44_11600"
     CDS             6294..8264
                     /locus_tag="VFY44_11600"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEX5911128.1"
                     /translation="MPRRTPILIATILACACLPASAAGAPPKAQPLSSGWEIRDLVNT
                     PPAPTPPPPSESGEGETGSTESAPVPVEPFNPNWKSAQVPGVFAADANPSLFGGTAKQ
                     YRLRFTAPPRAAGYTWAFRFEGSHRRTTAFLNGRKLGVSIDPYTPFELPAKGLRAGRA
                     NDLVVTVDSRKDPRLPEAWWNYGGIVRPVSLIPKGRIGVKDLAFLSNVTCKGLATQCK
                     AGVKLDGILSKLPKERQRYRTKVRMVGKGKKRRRVVTRTKIPLTQPKLVVKLRSPQGR
                     LTRKSLKLSGLRSARRRLKDLEVRVPAPKLWSPDNPQLYSASASLVYKNRTEQIDRKK
                     IGLRSVKVVRGELLLNNRPINIRGGSIHEDMPNHGAALTASDMDTIVRELKEVGANTT
                     RAHYLMNEGLLERFDRAGIMVWNQAPIWQRDHGANLLQYGNERKRAMAQMERTVTGAR
                     NHPSVITHSVSNELSFTPDRKAGTRRYLIDAAEKARDLDPTLPISLDIKGRPGFEEQF
                     VYENFDMIGINQYFGWYPWVEDFNTLEPYLREMNDLYPNHAKVMTEFGAEGRADMQDF
                     PPETLGTYAFQANHVARTVDLIDRLPFMTGAIHWTMREFEINPGWLGGVRIGRPGRNT
                     RHYKGVLTYKGLRKPAWTALHDRYVRTPLYRR"
     gene            8359..>9586
                     /locus_tag="VFY44_11605"
     CDS             8359..>9586
                     /locus_tag="VFY44_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911129.1"
                     /translation="MTKQSFQLLACALLTALVALLAPASASAATNQLSLLQDDREFLG
                     GTGENLDSAAAELKNLGVDILRTNVNYNKVYRTPKDRKKPSDFVTSDPDNRHYDWSAM
                     DRLVALAKANDVKVLMTVTGPGPFFSSEKPSKCRKLPCTYKPRTAEFEQFAAAVAKRY
                     RGKADYYAIWNEPNIGKTWLTPRYEKRRGVGTIDYAAAKYRQLYFAGYKSIAKYDPAR
                     KNRVLFGETAAISKPIPFLRAALCLDARGNPFRGRRAKAQGCSGRVKKVNTLGFAHHP
                     YNQGGNGTPRSKSRTSTSISVAYTPRLIRLIDGAARKGRIARRRGVYITEFGYQSNPP
                     DRISNVSPTEQGQYLNESDRLFYGSSRVKWVGQYELTDVPQQDQFNSGLRFVGGKVKP
                     AYAAYRMPIVVTRRSAN"
BASE COUNT         1544 a         3457 c         3284 g         1301 t
ORIGIN      
        1 ccagctcgaa gccgatccgc ccgctgaggc aggccagcgc gcccgccgcc tcgccccggt
       61 ccagcaggag cgctccgatg gccttgtcca tcgcgttgtg gcgcccgacg tcctcgcgca
      121 ggacgagcag ctcgcccgca gcggtgaaca gcgccgtggc gtggatcccg ccggtgcggt
      181 cgaacgcatc ctgggccggc cgcgcccggc cgggcaggtc cagcacctgg gccggctcga
      241 tccgcacggg cgccggtggc cgggcgggca cctccaggcg cacctgctcc agcgcgccct
      301 tgccacacac cccgcacgag gacgtcatgt ggaaccggcg ctcggcgaac gggtcgcgtc
      361 gcagccccgc gcgggtggtc acctcgacga tgttggcctc cagctcggcg ttcgggccgg
      421 cctcggccac gtcctcgcga cccgcgatca gcccttcccc cagcaggaac cccaccgcca
      481 gctcctcgtc cgcgccgggt gtgcgcatgg tcaccgccag ggcccgggcg tcgacgcgga
      541 tctccagggg ctcctcgatg gccacctcgt cgcgcaccag ctcgcgctgc tcgcccgcga
      601 cccgctcgat ccaggtgcgc gcgacgccgg agtcgatggc ctcgggcatg gcagaaaggc
      661 taaccgggtg gaggcggccc cggaaatggg aagaccccgc ctgagcgggg ccttccggta
      721 gctggcctgt aagccggatc ctgtcgtgga cagtcatcca tctatgcggc ctacctggga
      781 ctcggcgggc agcgtcgacg tccctgcttg gccttgcacc tggtggggtt tgcctggccg
      841 ccgtgtcacc acgacgccgg tgcgctctta ccgcaccatt tcacccttac cggcgacggg
      901 ggataacact cccagcgcag ccggctagcc ggctgcttcg ggcctgtgag gccccgccgc
      961 ttaggcggtg tcttttctgt ggcactttcc cgcgggtttc cccgggtcgg cgttaccgac
     1021 caccatgccc tgcggtgtcc ggactttcct cgaaccggtt ttctctccgg cccgcaacca
     1081 tccagccagc aacgttcgag tgtaggacgg cgtaggcggc ttgcctagcc gatggccgtg
     1141 ccgcagccgc cgcaccggaa ggccgggtgc gggtccgagg cgtgcgtggg cgccgtcagg
     1201 cgcgtccact ggccatcgga cccgcacgcc ggacagctgg cgttgcggtt ggcggtcagg
     1261 ccctcccata cgcgctcgag gtgctgctcg agcgtcgtcg gcctgccgcc ggaggggtcg
     1321 ggacgacgcc gcggagcgtc gtcccgaccg ggcgggcggc gggtgtcccg gaagagccgg
     1381 ggccgttcta cgacggccgt gctcatgaga ccctcctgag gactcccacc accctgccga
     1441 gcacctgcac ctcatcgctg aggatgggct cgagcagatc gttctcgggc tgcaacctga
     1501 tgtggtcgct ctcccggaag aagcgcttgc atgtggcctc ggcgtcaccg accatcgcca
     1561 cgacgatctc gccgtcgctg gcggtctcct gtgagcggac caccacgtga tcgccgtcgt
     1621 ggatccctgc gttgatcatc gagtcaccgg tgatggcgag gatgaactcg cccttgtcgc
     1681 cgccggccag ctcggggatc tgcacgtact cctcgatgtt ctcctcggcc aggatcggcg
     1741 agccggcggc cacctggccg acgaccggga gcgctccgcc ggcggcggtc tcgaccgcgg
     1801 tcttgacccg cttggccttg tcgaccagca gctcgatggc gcgcggcttg gtgggatcgc
     1861 ggcgcagcag cccggccttc tcgaggttgg ccaggtgggc gtgcacggtg gacgacgacg
     1921 tgaggccgat ggccttgccg atgtcccgca ccgtgggcgg gtagccgtac ttggccgagt
     1981 accgcccgat gaaatcgaag atctcctgct ggcgcttggt caggttgagg tccttcagtt
     2041 cggccatgtg gcacctcttc cggtggcttt cgagtgcgct tcatcgaaca tgtgttcgta
     2101 gagtaccccc gcgtcggacg gaacgaggga ggccacccgc caccgctatg atcggcgccc
     2161 gttcgcgcgc ctgtggcgga attggtagac gcgccagctt gaggggctgg tgatcgaaag
     2221 atcgtggagg ttcgagtcct ctcgggcgca cttgacgcga agccccgccg ctggttgtct
     2281 ctgggccgga gtgggtcata cggggggaaa cgcagacagc ggtgtcgggc cgctgctggt
     2341 ggtcgagcgc tcgggcggcg tcgccgggcg cttcgcgcgc atgcgcgtgg atccgtgcgg
     2401 caccgtggcc gtgcgccgcc cccccgagga cgaggtgacc ctccagctcg acgagcgccg
     2461 gctggcgcac ctgcgctcgc tggtcaccgg atcgcatttc cggtcgctgt cggcgaccta
     2521 cttcctcccc cgccccgccg ttcccggcgg ctacgcgtgg cgcatcagcc acgccgggac
     2581 gaccgtcgcc acccgcgacg gcgaggcgcc gcgcggcctg ggcgccctca tgcacgagct
     2641 cgccgcgctg atcgacgacg ccgtgttcgc cggcgggcat cgctaatccg tccataacgt
     2701 tcaaggcgca acccgtcctg ccgaacgggg ttcttaggag cactcacacg aacccatttc
     2761 gcaggagcac gatgccccag acacagacag cctcgagcca cagcgccccc ggctggtacc
     2821 cggaccccag gggccggggc cagcgctact gggacggaca gggatggacg cgcatgcgcc
     2881 gcgtcagccc ccgcaccacc agcaccggcg gcggcgcctg ggtcggcctg aacggccggc
     2941 cgaggtcgac aggcggctac tgaacaaggt cggccgaagc aacggccaag aggcggcgta
     3001 gccgccggtc tcggcgccgg gacggcgccc tcccctcaaa gcgcggccat ggatggccgc
     3061 aagagctaga actagatctt caggaggact atcacgttca ccacgaacag cgtggcgaac
     3121 gcgatcgtcc agcggttcag gttgcgctcg accagggagc cgccgccgag ggggccgctg
     3181 cccccgccaa cgccgaaggc gccggagagg ccggtgtcct tgcccgagtg cagcagcacc
     3241 agcacgatca ggacgatcga gagcaccacc tgcacgccgc tgagaatcgc ttccatcgcg
     3301 gcagtacggt agcggctagg cccgcggggc ctcgccctcc agctcgtcga ggcggcgcag
     3361 gatgtcgatg gcgtcggccc gcagctcgcg gtcgatggcc cggtggtcct ccgcggtgag
     3421 cttgcccatc tggcggtcga gctcggcgtc gcggatctcg cggtacttgg cctccttggc
     3481 cgcctccagc tctgcgcggg cggcgcccac gtcggcctcg tcgtcctcgc ggtagaccac
     3541 ggggcgcacg acgatcaggg tcaccacggc gaccaggaag atgagggcga ggccgtactc
     3601 cacggccccg caccctagga ggcgcggccc gcgagctcct tgcccaggcg ggcggagtag
     3661 aggctgcgca cccgccgcag cttggcctcg ggggacggcc aggcggccac cagcgcgcca
     3721 aggaggacga tgccgccgcc gatccagatc cacgacacca gcggcgagac gatcgcccgg
     3781 aacgtcgccg gcggcgggtt gcggcggtag ttctcggcca gggcgccgat gagcagcgcc
     3841 tgggtgtcgg ccgtcggctg ggggaacttg cggtcggcgt cgcggatggg cttgtcgagc
     3901 gagctgatgt cgggccgcat ggccagccag aagtcacgcc gcaggcccca gcgcacgtcg
     3961 acctcgctgg tggcctcgcc ctcgaagtac cgcgcggtcg cgcccttggt ggggtcctgg
     4021 gtcgggaagt agttgcgcga ggggtgcagg gtcttgacct cgtcgccctt gcggacgcgc
     4081 atcacggcgc cgaacgagat cggcgcgccg gtgcccgcgt cgtcggcccc caggcgcgcg
     4141 gtcggcctga cgtaggtcac gcggtagtcg ccctggctga agctctggcc gggcgagagc
     4201 cgcacgtcct tctgctggtg gaacgccgac gagccggcga cgcccaggaa cagcacggcg
     4261 atgccggcgt ggacgatgta gccgccgtag cggcgccggt tgcggcccac cagctggccg
     4321 aacgcggtgt gcaggggctc gccggtgagc tgccggcggg ccagggcgcc gcgggcgaac
     4381 tcctggacca cgacgcccag cagcagggcg acgagcccga acatggccag cgcggtgagg
     4441 ctgtcgggcg cgttggtggc caacgccagc acgatgagca cgaggaccgc gaacgcgaac
     4501 ggcgcgcgca ccgtccgcca caggcccccg gcgctgacgc ggcgccaggc cagcatcggg
     4561 ccgagcgtca tgagcgccac cagcaccagc gccagcgggg tgacgacccg gttgaaccag
     4621 ggcgggccga cgctggcctc ggtgccggtc acggcctcgg agatcagcgg gaagaaggtc
     4681 ccccagaaga ccacgagcgc cagcgcgacc aggatcaggt tgttcagcag gaagaaggcc
     4741 tcgcgcgaga tcagcgagtc cagccgcgac gtgctgcgca ggtcgggcag gcgccagacc
     4801 acgagcgcca cggagaacac gatgaccagc gcgatgaagg tcaggaactg ggtcccgatg
     4861 gtggacgcgc cgaacgcgtg gatggactcc aggatccccg accggaccag gaacgtgccc
     4921 agcagcgcga gcacgaacgt ggcgatgatc agcgacacgt tccagacctt cagcatcccg
     4981 cgcttctcct ggaccatgac ggagtgcagg aacgcggtgc cggtcagcca gggcagcagc
     5041 gaggcgttct cgaccgggtc ccaggcccag tagccgcccc agcccagctc ggtgtaggac
     5101 cacagcgcgc cgagcatgat gcccaccccg aggaaggtcc acgcgacgag cgcgaaccgg
     5161 cgcgtggcgc ggatccagtc ggcgccggtg gagcgggtga tcagggcgcc gacggcgaag
     5221 gcgaacggga tcgagaaccc gacgtagccg gtgtagagca tcggcgggtg gatcatcatc
     5281 gccgggtggc gcagcagcgg gttcaggccc acgccctcgg cgggcgggtt ggccagggtg
     5341 gtgaacgggt tctcccagcc caccatcagc agcaggaaga aggcggcgac cacgcccagg
     5401 accgcggtgg cccacggcgc gatctcgcgc agcgagcggc gggtcaggaa caggacgatg
     5461 ctggtgaaga tcgacagcag cagcgcccac agcagcagcg agccgtcctg ggtcgcccac
     5521 atggcggtga ccttgtagaa ggtcggcgtg tcggtggacg agccgtcggc caccagcttg
     5581 aacgagaagt ccgagcgcag gaacgcggac tgcaggatgg ccgtggcccc gatgcagagc
     5641 gcggccacgc agtacatggc gcgccggccc gagaccacga agtcgcgccg gcccgagacg
     5701 gccccgtaga ccgaggagcc ggccgcgtac accgcggtca gcagcgcgac gatcaggcag
     5761 gcggagccga cccccgccac gctacgtctg cttcttcttg tcctcggtga acttcgaggg
     5821 gcacttggtg accagcgagt cgcgctcggc ggcgaaacgc ctgccctcga gcttgcccga
     5881 gacgatgacc tcgcgcccct cgcggaacgg gtcgggcacc acgccggcgt agctcaccgg
     5941 gacggacgcg gtgccgtcgc ggtcgcggat ggcgaactcc agtcggtccc ccacgcgctt
     6001 cacggaatcg ttcacgacct tgccggtgag ctcgtacgag cgtccgggct cggcgttctt
     6061 catgacctcg gacggggtac tggcttccgt cgaggcgctg aagctggtgt agaccagcac
     6121 gcctgcaaga agtacggcaa cggagagtgc aagcacgagc cgtgccctac gtttccggga
     6181 ggggtccatc gagtagaagt gtacgaggcg ccgccgcccg gccttctgcc gatcggcgcc
     6241 aattgtcggg tcacagcaca accaggggga ctttcagggg ttacatgcag ccagtgccgc
     6301 gccgaacgcc gatactcatc gccacgatcc tggcctgtgc ctgcctgccc gcgagcgcgg
     6361 ccggcgcgcc gccgaaggcc cagccgctct ccagcggctg ggagatccgc gacctggtga
     6421 acacgccgcc ggcccccacc ccgccaccgc cgtcggagtc cggcgagggc gagaccggct
     6481 ccaccgagag cgccccggtg ccggtcgagc cgttcaaccc gaactggaag tccgcccagg
     6541 tgccgggcgt gttcgcggcc gacgccaacc cgtcgctgtt cggcggcacc gccaagcagt
     6601 accggctccg cttcaccgcg ccccccaggg ccgcgggcta cacctgggcc ttccgcttcg
     6661 agggctcgca ccggcgcacc accgccttcc tgaacgggcg caagctcggc gtcagcatcg
     6721 acccctacac cccgttcgag ctccccgcca agggcctgcg cgcgggccgc gccaacgacc
     6781 tggtggtcac cgtcgacagc cgcaaggacc cgcgcctgcc cgaggcctgg tggaactacg
     6841 gcggcatcgt ccgccccgtc tcgctgatcc ccaagggccg gatcggcgtc aaggacctgg
     6901 ccttcctgag caacgtgacc tgcaagggcc tggccaccca gtgcaaggcc ggggtgaagc
     6961 tggacgggat cctgtccaag ctgcccaagg agcgccagcg ctaccggacg aaggtccgca
     7021 tggtgggcaa gggcaagaag cgccgccgcg tggtcacgcg gacgaagatc cccctgaccc
     7081 agcccaagct ggtggtcaag ctgcgctcgc cccagggccg gctgacgcgc aagagcctca
     7141 agctcagcgg cctgcgcagc gcgcgccgcc ggctcaagga cctcgaggtg cgggtcccgg
     7201 cgcccaagct ctggtcgccc gacaacccgc agctgtactc ggcgtcggcg agcctggtct
     7261 acaagaaccg caccgagcag atcgatcgca agaagatcgg cctgcggtcg gtcaaggtcg
     7321 tccgcggcga gctgctgctg aacaaccgcc cgatcaacat ccgcggcggc tcgatccacg
     7381 aggacatgcc caaccacggc gcggcgctga cggcctcgga catggacacg atcgtgcgcg
     7441 agctcaagga ggtgggcgcc aacaccacgc gcgcccacta cctgatgaac gagggcctgc
     7501 tggagcgctt cgaccgggcc gggatcatgg tctggaacca ggcgccgatc tggcagcgcg
     7561 accacggcgc caacctgctc cagtacggca acgagcgcaa gcgcgcgatg gcgcagatgg
     7621 agcgcacggt cacgggcgcg cgcaaccacc cgtcggtgat cacccactcg gtctccaacg
     7681 agctgagctt cacccccgac cgcaaggccg gcacccgccg ctacctgatc gacgccgccg
     7741 agaaggcgcg cgacctggac cccacgctcc cgatcagcct ggacatcaag ggccggccgg
     7801 gcttcgagga gcagttcgtc tacgagaact tcgacatgat cggcatcaac cagtacttcg
     7861 gctggtatcc gtgggtcgag gacttcaaca cgctggagcc ctacctgcgt gagatgaacg
     7921 acctctaccc caaccacgcc aaggtcatga ccgagttcgg cgccgagggc agggccgaca
     7981 tgcaggactt cccgcccgag acgctgggca cctacgcctt ccaggccaac cacgtggccc
     8041 ggaccgtgga cctgatcgac cggctgccgt tcatgaccgg cgccatccac tggaccatgc
     8101 gcgagttcga gatcaacccg ggctggctgg gcggcgtccg catcggccgc ccggggcgga
     8161 acacccgtca ttacaagggc gtcctgacct acaagggcct gcgcaagccg gcctggacgg
     8221 ccctgcacga ccgctacgtt cgcaccccgc tgtaccggcg ctagagcgca acttccgccc
     8281 cgcgggcgtg tgtactatct ccgaccccct ccgggcgctc cccaggcccg ccgtacccga
     8341 ccgaggaaca cccttcatat gacgaagcag agcttccaac tgctggcctg cgcgctgctg
     8401 acggcgctcg tcgcgctgct ggcgcccgcc agcgcctccg ccgcgaccaa ccagctgtcg
     8461 ctgctgcagg acgatcgcga gttcctgggc gggaccggcg agaacctgga ctcggccgcg
     8521 gccgagctga agaacctcgg cgtggacatc ctccgcacca acgtcaacta caacaaggtc
     8581 taccggacgc ccaaggaccg caagaagccc tcggacttcg tcaccagcga tcccgacaac
     8641 cggcactacg actggtccgc catggaccgc ctggtcgcgc tggccaaggc caacgacgtg
     8701 aaggtcctga tgaccgtcac cggccccggc cccttcttct cctcggagaa gccgtccaag
     8761 tgccggaagc tgccgtgcac ctacaagccc aggaccgccg agttcgagca gttcgccgcg
     8821 gccgtggcca agcgctaccg gggcaaggcc gactactacg ccatctggaa cgagccgaac
     8881 atcggcaaga cctggctgac cccgcgttac gagaagcgcc gcggcgtcgg cacgatcgac
     8941 tacgccgccg ccaagtaccg ccagctgtac ttcgccggct acaagtcgat cgccaagtac
     9001 gacccggcgc gcaagaaccg cgtcctgttc ggcgagaccg cggccatcag caagccgatc
     9061 ccgttcctgc gcgccgcgct ctgcctggac gccaggggca acccgttccg cggccggcgc
     9121 gccaaggccc agggctgctc gggccgcgtg aagaaggtca acacgctggg cttcgcccac
     9181 cacccgtaca accagggcgg caacggcacc ccgcggagca agagccgcac gtcgacctcg
     9241 atctcggtgg cctacacccc gcgcctgatc cgcctgatcg acggcgccgc gcgcaagggg
     9301 cggatcgccc gccgccgcgg cgtgtacatc acggagttcg gctaccagtc caacccgccg
     9361 gaccggatct ccaacgtcag ccccaccgag cagggccagt acctgaacga gtccgaccgc
     9421 ctgttctacg gctccagccg cgtgaagtgg gtgggccagt acgagctcac cgacgtgccc
     9481 cagcaggacc agttcaactc cgggctgcgg ttcgtgggcg gcaaggtcaa gcccgcctac
     9541 gcggcgtacc ggatgccgat cgtggtcacg cgccgctcgg ccaact
//
LOCUS       DASUPW010000517         4853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1782434, whole genome shotgun sequence.
ACCESSION   DASUPW010000517 DASUPW010000000
VERSION     DASUPW010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1782434"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VFY44_11610"
     CDS             <1..261
                     /locus_tag="VFY44_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911130.1"
                     /translation="RLAVRGEPLIIASGKLEHREGVTNVVVHDVQRLDRGDLPQAEIR
                     HIEPRRTWSSESGEVVPGTPGARTADLREVAPAANSFGRGRR"
     gene            279..518
                     /locus_tag="VFY44_11615"
     CDS             279..518
                     /locus_tag="VFY44_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911131.1"
                     /translation="MTRNEAAREAARLAEENPGRRWLPREGDDGEWTVAEIKLPAGMK
                     PNPPLKETTEERPRPPQADDVRPAHDRNVGGPYGF"
     gene            complement(515..1522)
                     /locus_tag="VFY44_11620"
     CDS             complement(515..1522)
                     /locus_tag="VFY44_11620"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX5911132.1"
                     /translation="MALPAEVWKLDDISPKAYQHPADRATTAALANVPYLDQVVRKLV
                     ALGYERSLRTATLGAAVRLGDNQLPHVHKLQREVFNVLDHEPVPDLYITQNPMANAFT
                     GGSEKPVVVMNSEIVRLLDEDGQRVVLAHEAAHVHSDHVLYLTALAILKQLVSGTVAR
                     IPMLAGLPMVAVYYALLEWSRSAELSCDRAAALVTRDPEAVCRALMVITAGEAAEHLS
                     LDAFIAQGMEYQDVGGAAKLTRMMQDLNVTHPLPVRRVHYLLDWVKGGEYEAMIGGEY
                     LRRGQEPSVSEEASAARDHYADRISSAVQEAGSSVAEVGQQLGDWLNRQRGGDSDGSK
                     G"
     gene            1538..2458
                     /locus_tag="VFY44_11625"
     CDS             1538..2458
                     /locus_tag="VFY44_11625"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX5911133.1"
                     /translation="MAGRIVVGTSSWADPGFIKDWYPQGMKAADRLPWYAERFEAVEL
                     NSSFYAVPEQKTVARWAQITPDGFTFDVKLHRLLSRHSAGLESLPPDMRDGAGVTARG
                     RVQLTPELESALTGQILEAVEPLREAGKLGGFLLQMTPGFSPKRNQLSELEPLVSAFA
                     PHRLALELRNRRWVDEERIEQTLEWFEDQNVAFVCVDSPPGDATTIMPGVDAATRDDL
                     AYLRMHGRDTQAYLSGKTVAERFGWIYGDDELEEIATRAKGLAEEAAEVHVFFNNNRD
                     DDAPTAARRFRALLGQDPGPPPEDDQLSLV"
     gene            complement(2455..2643)
                     /locus_tag="VFY44_11630"
     CDS             complement(2455..2643)
                     /locus_tag="VFY44_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911134.1"
                     /translation="MDRPPPSPSRFALVPRASHGANGGPRIKRYSLARLQPSPRSALG
                     EDRHERLERAAGRPPHRA"
     gene            complement(2716..3126)
                     /locus_tag="VFY44_11635"
     CDS             complement(2716..3126)
                     /locus_tag="VFY44_11635"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5911135.1"
                     /translation="MDKQTSGRTTSGAESVRVQITGDSGAAARARAELARLRSMLDDP
                     ILENVRLLVTELVTNSVRHARASDIELLVVVSRTRVHVEVANPGGGFEPRPSGPEDSE
                     TGWGLFLVDRLSDDWGVTQGTGADYQRVWFELSR"
     gene            3202..3933
                     /locus_tag="VFY44_11640"
     CDS             3202..3933
                     /locus_tag="VFY44_11640"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5911136.1"
                     /translation="MADLDPTGIRRVRADNPSPLTLDGTNTYVVGRWVVDPGPDDPAH
                     LEAVLAACEGPLEGIALTHTHPDHAEGAPALAAMAGGVDVVLGVEGQRVGPFDVIATP
                     GHAPDHVCLLLDGRIAFAGDTVLGAGSVFVGPGDGSLAAYLASLRRLRELDLDVICPG
                     HGPYVEDPAAKLDEYLHHRLDRERRLLEALDAGAREEGALLDHAWSDVPPQLRHAAGL
                     TLRAHLEKLAQEDLLPPGLDPTLPG"
     gene            3939..>4853
                     /locus_tag="VFY44_11645"
     CDS             3939..>4853
                     /locus_tag="VFY44_11645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX5911137.1"
                     /translation="MRIVVATEAGDPDVLQVQEVPVPEPIPTEVLVRVHAAGVNPVDW
                     KTRQGKGMAGVLGDAPWRIGWDVAGVVEEVGMGVTRFRPGDEVFGMPWFPREAGAYAD
                     YVTCPARQLALRPEGLDFAAAAALPLAALTAWQALVDTAGLQAGQRVLVHAAAGGVGH
                     LAVQIAKALGAEVFGTASAGKHDFLRELGVDRPIDYKAEAFEDVATDVDVVLDLIGTW
                     DYGTRSLACLREGGTLLEIPSGADPGVLEAADEQNKRATGMLVEPDGAGLERIAQLCR
                     DGKLAPVLEEPLPLEQAARAHELGEQGRT"
BASE COUNT          669 a         1718 c         1834 g          632 t
ORIGIN      
        1 cgcctggccg tgcgcggcga gccgctgatc atcgcctcgg gcaagctcga gcaccgcgag
       61 ggcgtgacca acgtggtggt gcacgacgtc cagcgcctgg accgaggcga cctgccccag
      121 gccgagatcc gccacatcga gccgcggcgt acgtggagct cggagtcggg cgaggtcgtg
      181 ccgggcacgc cgggggcccg gaccgccgac ctgcgcgagg tggcgccggc cgcgaactcc
      241 ttcggcaggg gccggcgctg agggcctaga gttgagccat gacccgcaac gaggccgcac
      301 gagaggcagc gaggctggcg gaggagaacc cgggccggcg ctggctgccg cgcgagggcg
      361 acgacggcga gtggacggtg gcggagatca agctgccggc gggcatgaag cccaacccgc
      421 cgctgaagga gaccaccgag gagcggcccc ggccgccgca ggccgacgac gtgcgccccg
      481 cgcacgaccg caacgtcggc gggccgtacg gcttctagcc cttcgagccg tccgagtcgc
      541 cgccgcgctg gcggttgagc cagtcgccca gctgctggcc gacctcggcc accgacgagc
      601 ccgcctcctg caccgcggag gagatccggt cggcgtagtg gtcgcgggcg gcgctcgcct
      661 cctccgagac cgacggctcc tggccgcggc gcaggtactc gccgccgatc atggcctcgt
      721 actcgccgcc cttgacccag tcgagcaggt agtggacacg gcgcaccggc agtgggtggg
      781 tcacgttcag gtcctgcatc atccgcgtga gcttggccgc gccgccgacg tcctggtact
      841 ccatgccctg ggcgatgaac gcgtccaggg acaggtgctc ggcggcctcg ccggcggtga
      901 tgaccatgag cgcgcggcag acggcctcgg ggtcgcgggt gaccagggcg gcggcgcggt
      961 cgcagctcag ctcggccgag cgcgaccact ccagcagcgc gtagtagacg gccaccatgg
     1021 gcagcccggc cagcatcggg atgcgcgcga ccgtgcccga caccagctgc ttgaggatgg
     1081 ccagcgcggt caggtagagc acgtggtcgg agtgcacgtg cgcggcctcg tgggccagga
     1141 cgacgcgctg gccgtcctcg tccagcagcc ggacgatctc cgaattcatg accaccaccg
     1201 gcttctcgct gccgccggtg aacgcgttgg ccatcgggtt ctgggtgatg tacaggtccg
     1261 gcaccggctc gtggtccagc acgttgaaga cctcgcgctg gagcttgtgg acgtggggaa
     1321 gctggttgtc gccgaggcgc accgcggcgc ccagcgtggc ggtgcgcagg ctgcgctcgt
     1381 agcccagcgc cacgagcttt cgcaccacct ggtccaggta cggcacgttg gccagcgccg
     1441 ccgtggtcgc ccggtcggcc ggatgctggt aggccttggg ggagatgtcg tccagcttcc
     1501 agacctccgc gggtagcgcc atgggcggag cgtagtcatg gccggtcgca tcgtcgtcgg
     1561 cacctcgtcc tgggccgatc ccggcttcat caaggactgg tacccgcagg gcatgaaggc
     1621 cgcggaccgg ctgccctggt acgccgagcg gttcgaggcg gtggagctga actccagctt
     1681 ctacgccgtc cccgagcaga agaccgtggc gcgctgggcc cagatcacgc ccgacgggtt
     1741 caccttcgac gtgaagctcc accggctgct gtcgcggcac tcggccgggc tggagtcgct
     1801 gccgcccgac atgcgcgacg gcgccggcgt gaccgcgcgc gggcgcgtgc agctgacgcc
     1861 cgagctggag tcggcgctga ccggccagat cctggaggcg gtcgagccgc tgcgcgaagc
     1921 gggcaagctg ggcgggttcc tgctgcagat gacgccgggc ttctcgccca agcgcaacca
     1981 gctgtccgag ctggagccgc tggtgtcggc gttcgcgccc caccggctgg cgctggagct
     2041 gcgcaaccgc cgctgggtgg acgaggagcg gatcgagcag acgctggagt ggttcgagga
     2101 ccagaacgtg gccttcgtgt gcgtggactc cccgcccggc gacgccacca cgatcatgcc
     2161 gggggtggac gcggccaccc gcgacgacct ggcgtacctg cgcatgcacg ggcgcgacac
     2221 ccaggcctac ctcagcggca agaccgtggc cgagcggttc ggctggatct acggcgacga
     2281 cgagctggag gagatcgcca cccgggcgaa gggcctggcc gaggaggcgg ccgaggtgca
     2341 cgtgttcttc aacaacaacc gcgacgacga cgccccgacc gcggcgcgcc ggttccgcgc
     2401 tctgctcggc caggaccccg ggccgccgcc cgaggacgac cagctgtcgc tggtctaggc
     2461 gcggtgcggc gggcgcccgg ccgcccgttc gagacgctcg tgccggtcct cacccagcgc
     2521 cgaccgcggt gagggctgca ggcgcgccag gctgtagcgc ttgatgcgcg ggccgccgtt
     2581 ggcgccgtgc gaggcccgcg gcaccagcgc gaaccggctg ggggatggcg gcggcctgtc
     2641 catgacagac aggatgcgcc gcaaacgaat ggttttcttg ggccgccggt ccgaagatcg
     2701 gcgcggccgg ctcgactagc gggacagttc gaaccagacg cgctggtagt cggcgccggt
     2761 tccctgggtg acgccccagt cgtcgctgag ccggtcgacc aggaacaggc cccagcccgt
     2821 ctcggagtcc tcggggccgg agggccgtgg ctcgaaaccg ccgccggggt tggccacctc
     2881 gacgtggacg cgtgtacgag agaccaccac caggagctcg atgtcggacg cccgggcgtg
     2941 gcgcacggag ttggtcacca gctcggtgac caggaggcgg acgttctcca ggatcgggtc
     3001 gtccagcatc gagcgcaggc gcgccaattc ggctctggca cgggccgcag cgccgctgtc
     3061 gccggtgatc tgcacgcgca cgctctccgc gcccgaggtg gtcctacctg aagtctgttt
     3121 gtccattagg cgccccacgt cttggtgttc cccgaaacgg ccggaaacca agcgtgcgaa
     3181 ggctcacgca agaatccccc catggccgac ctcgacccaa ccggcatccg gcgcgtccgg
     3241 gcggacaacc cgtcaccgct gacgctggac ggcaccaaca cctacgtcgt ggggcggtgg
     3301 gtcgtcgacc ccgggcccga tgacccggcc cacctggagg ccgtgctggc cgcctgcgag
     3361 ggaccgctcg agggcatcgc cctgacccac acgcaccccg accacgccga gggggcgccg
     3421 gcgctggcgg ccatggcggg aggcgtcgac gtcgtgctgg gcgtcgaggg ccagcgggtc
     3481 ggccccttcg acgtgatcgc caccccgggc cacgctccgg atcatgtgtg cctgctcctg
     3541 gacggccgga tcgccttcgc gggcgacacc gtgctggggg cgggcagcgt gttcgtgggg
     3601 ccgggggacg gatcgctggc ggcctacctg gcctcgctcc ggcggctgcg cgagctggac
     3661 ctggacgtga tctgcccggg gcacggcccc tacgtggagg acccggcggc gaagctcgac
     3721 gagtacctgc accaccgcct ggaccgcgag cgcaggctgc tcgaggcgct ggacgcgggg
     3781 gcgcgtgagg agggggcgct gctggaccac gcctggtccg acgtgccgcc ccagctgcgc
     3841 cacgcggccg gcctgacgct gcgtgcccac ctggagaagc tggcgcagga ggacctcctg
     3901 ccgccgggcc tcgatccgac cctgccaggc tgaacggcat gcgcatcgtc gtcgccaccg
     3961 aggccggaga ccccgacgtc ctgcaggtcc aggaggtgcc ggtccccgag cccatcccca
     4021 ccgaggtgct ggtccgggtg cacgccgccg gcgtgaaccc ggtggactgg aagacgcgcc
     4081 agggcaaggg catggccggc gtgctgggcg acgcgccgtg gcggatcggc tgggacgtcg
     4141 cgggcgtggt cgaggaggtg ggcatgggcg tcacgcgctt ccgccccggc gacgaggtgt
     4201 tcggcatgcc ctggttcccg cgcgaggcgg gcgcctacgc cgactacgtg acctgccccg
     4261 cgcgccagct ggcgctgcgg cccgagggcc tggacttcgc cgccgcggcc gcgctgcccc
     4321 tggcggccct gacggcgtgg caggcgctgg tggacaccgc cggccttcag gccggccagc
     4381 gtgtgctcgt gcacgccgcc gcgggcggcg tgggccacct ggcggtgcag atcgccaagg
     4441 cgctgggcgc cgaggtcttc ggcacggcca gcgccggcaa gcacgacttc ctgcgtgagc
     4501 tcggcgtgga ccgcccgatc gactacaagg ccgaggcgtt cgaggacgtg gccaccgacg
     4561 tcgacgtggt gctggacctg atcggcacct gggactacgg cacccgttcg ctggcgtgcc
     4621 tgcgcgaagg cggcacgctc ctggagatcc cctccggcgc cgatcccggc gtgctcgagg
     4681 ccgccgacga gcagaacaag cgcgccacgg ggatgctggt cgagcccgac ggcgccgggc
     4741 tggagcggat cgcccagctg tgccgcgacg gcaagctggc gcccgtgctc gaggagccgc
     4801 tgccgctgga gcaggcggcg cgcgcccacg agctgggcga gcaggggcgc acg
//
LOCUS       DASUPW010000518         4322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1229492, whole genome shotgun sequence.
ACCESSION   DASUPW010000518 DASUPW010000000
VERSION     DASUPW010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229492"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VFY44_11650"
     CDS             complement(<1..795)
                     /locus_tag="VFY44_11650"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEX5911138.1"
                     /translation="MSDEPIPLARPVIGEREEELVLQTLRSGQIGIGPRVAEFEDELG
                     RVLGTEHVSAISSGTAALHLAVRASGIEAGDEVVTSPFSFVASSNCVIYEDATPVFCD
                     IDPRTLNIAPEAAAAAVTERTTGLLPVHIFGYPADMPGFERLASERGLWLVEDACEAL
                     GAVHADGTTVGARGNPATFGFYPNKQLATGEGGALASPDAEFKARVDSERNQGRAPDM
                     GWLDHDRLGYNYRLSAIAAALGLAQVERLEAMLADRARVAGLYDQAL"
     gene            complement(792..2387)
                     /gene="cimA"
                     /locus_tag="VFY44_11655"
     CDS             complement(792..2387)
                     /gene="cimA"
                     /locus_tag="VFY44_11655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEX5911139.1"
                     /translation="MAEQVLLYDTTLRDGMQGEGMSLSADEKVRVAHALDSLGVHMIE
                     AGFPGSNPKEIALFELLAGERFENAEIAAFGMTRRRDAAAEADEGLRVLADSFAPVCT
                     LVGKTWSLHLEKVTRVDPEENLRMIADSVAFLRGQGKRVVYDAEHFFDAWAGDSGYAL
                     RCLRAAVEAGAENITLCDTNGATLPTALGDAVSRVVAELGDHAQVGIHTHDDSGVGVA
                     NSLVAVEQGARMVQGTMNGYGERCGNANLVTILADLQLKLGFDCVAPDRLQHLTETAH
                     LVDELCNVAPNPDQPYVGANAFAHKGGMHVAGVNRDARTFEHIDPSQVGADRRLLISE
                     LSGRGTVQARADETGAGLDDEGAARVAQRVKDLESRGYQLEAADGSFDLLIRQEAGEY
                     ESLFRLESWRCIVEKREDGKVQTEATIKIWVEGERFVRTAEGNGPVHALDNALRDAIG
                     ETYPHLRDIELVNFKVRILDETKGTGAVTRVLLDASDGRDTWGAIGVSENVIEASWEA
                     LVDSLEAGMLPGRVDHTRAAVGR"
     gene            complement(2484..3410)
                     /locus_tag="VFY44_11660"
     CDS             complement(2484..3410)
                     /locus_tag="VFY44_11660"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX5911140.1"
                     /translation="MNNADQIWMNGEFVAWEDAKVHVLSHALHYGTSVFEGVRAYDTT
                     KGTAVFRHADHIKRLEESAKLYYMDLPFSREQIREATLESISRSGFSSCYIRPLVFRG
                     HGPMGLDPEDNPVEVSIAVFEWGAYLGDEGKQNGIRAKVSSWRRISPQSLVPHSKAGG
                     QYLNSVLAKIESKRGGYEEAILLDERGMVCEGSGENIFVVKDGQIATPPQTASILDGI
                     SRKSLIEIATDHGHTVVERDIARAELTLADEVFMVGTAAELVPVREIDDIEIGAPGEV
                     TRELQSAYEDALHGRTPAYEKWLDVVPVPSKA"
     gene            complement(3427..>4322)
                     /gene="leuB"
                     /locus_tag="VFY44_11665"
     CDS             complement(3427..>4322)
                     /gene="leuB"
                     /locus_tag="VFY44_11665"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX5911141.1"
                     /translation="TALTDEVLAACKQADAVLLAAVGGPKWDAAVAADPSAPRPEQGL
                     LGLRKGLGLFANLRPVRPSPALLHASPLKPERIQGTDLLVVRELTGGIYFGDSGRDGD
                     TAHDDCAYSVAEVERIARVGFESARTKVTSVDKANVLETSRLWREVVTRVHEQEFPDV
                     PLEHLLVDNAAMQLVSNPSDFDVIVTENLFGDILSDEAAMLTGSLGMLPSASIGGEGP
                     GLFEPVHGSAPDIAGTGKANPLAMFSSVALMLRHGLAMNEAAAAVESAVDGALGDGIR
                     TADLGGSHTTEDATKAVLQHL"
BASE COUNT          567 a         1531 c         1552 g          672 t
ORIGIN      
        1 cagcgcctgg tcgtacaggc cggccacgcg cgcccggtcg gccagcatgg cctcgaggcg
       61 ctcgacctgg gccagcccca gcgcggccgc gatggccgag aggcggtagt tgtagcccag
      121 gcggtcgtgg tcgagccagc ccatgtcggg cgcgcggccc tggttgcgct cgctgtccac
      181 gcgcgccttg aattccgcgt ccgggctggc cagggcgccg ccctcgcccg tggccagctg
      241 cttgttgggg tagaagccga aggtggccgg gttgccgcgc gcgcccaccg tcgtgccgtc
      301 ggcgtgcacg gcccccaggg cctcgcaggc gtcctcgacc agccacaggc cgcgctcgga
      361 ggccaggcgc tcgaagccgg gcatgtcggc cgggtagccg aagatgtgca cgggcaggag
      421 ccccgtggtg cgctcggtga ccgcggcggc cgccgcctcg ggggcgatgt tcagcgtgcg
      481 cgggtcgatg tcgcagaaca ccggcgtggc gtcctcgtag atcacgcagt tggacgacgc
      541 cacgaagctg aacgggctgg tgaccacctc gtcgccggcc tcgatccccg acgcgcgcac
      601 ggccaggtgc agcgccgcgg tgccgctgga gatggcggac acgtgctcgg tgcccagcac
      661 gcggcccagc tcgtcctcga actcggccac gcgcggcccg atgccgatct ggccggagcg
      721 cagcgtctgg agcacgagct cctcctcgcg ctcgccgatg accggccgcg cgagcgggat
      781 cggctcgtcg ctcaccgacc gaccgctgcc cgcgtgtggt ccacccggcc gggcagcatg
      841 cccgcctcga gcgagtcgac cagggcctcc cagctggcct cgatcacgtt ctcagagacg
      901 ccgatggcgc cccaggtgtc gcggccgtcg ctggcgtcca gcagcacgcg cgtgaccgcg
      961 ccggtgccct tggtctcgtc caggatgcgg accttgaagt tgaccagctc gatgtcgcgc
     1021 aggtgcgggt aggtctcgcc gatggcgtcg cgcagggcgt tgtcgagcgc gtgcaccggg
     1081 ccgttgccct ctgccgtgcg gacgaaccgc tcaccctcga cccagatctt gatcgtggcc
     1141 tcggtctgga ccttgccgtc ctcgcgcttc tcgacgatgc agcgccacga ctccagccgg
     1201 aacagcgact cgtactcacc ggcctcctgg cggatcagca ggtcgaacga gccgtcggcg
     1261 gcctcgagct ggtagccgcg gctctccagg tccttgaccc gctgcgccac ccgggccgcg
     1321 ccctcgtcgt ccagcccggc ccccgtctcg tcggcgcggg cctggaccgt gccccggccc
     1381 gagagctcgg agatcagcag ccgccggtcg gctcccacct gggacgggtc gatgtgctcg
     1441 aacgtgcgcg cgtcgcggtt cacgcccgcc acgtgcatcc cgcccttgtg ggcgaacgcg
     1501 ttggcgccca cgtagggctg gtcggggttc ggcgcgacgt tgcacagctc gtcgaccagg
     1561 tgggccgtct ccgtcaggtg ctggagcctg tccggggcca cgcagtcgaa gccgagcttg
     1621 agctggaggt cggccaggat ggtgaccagg ttggcgttgc cgcagcgctc gccgtagccg
     1681 ttcatcgtgc cctggaccat gcgcgcgccc tgctccacgg ccaccaggct gttggccacc
     1741 cccaccccgg agtcgtcgtg ggtgtggatg cccacctggg cgtggtcgcc gagctcggcg
     1801 accacgcgcg agacggcgtc accgagcgcg gtgggcagcg tggcaccgtt cgtgtcgcac
     1861 agggtgatgt tctcggcgcc cgcctcgacg gccgccctca ggcagcgcag cgcgtacccg
     1921 gagtcaccgg cccaggcgtc gaagaagtgc tcggcgtcgt agaccacgcg cttgccctgc
     1981 ccgcgcagga acgcgaccga gtcggcgatc atgcgcaggt tctcctccgg gtccacgcgg
     2041 gtgaccttct ccaggtgcag cgaccacgtc ttgcccacca gcgtgcacac gggcgcgaag
     2101 ctgtcggcca gcacgcgcag gccctcgtcg gcctcggcgg ccgcgtcgcg gcggcgggtc
     2161 atgccgaacg cggcgatctc ggcgttctcg aaccgctccc cggccagcag ctcgaacagc
     2221 gcgatctcct tcgggttcga gccggggaac ccggcctcga tcatgtgcac gcccagcgag
     2281 tcgagcgcgt gcgccacgcg caccttctcg tcggcggaca gcgacatgcc ctcgccctgc
     2341 atgccgtcgc ggagcgtcgt gtcgtatagg aggacctgct cggccatggt ggggaactgc
     2401 ctttctacgg tgcggcggaa gcggacgtgc ggcgtcgggg ccctaggacc cggacgcgcc
     2461 cgtaattcgc ttcgccaagg cgttcacgcc ttcgatggta caggcaccac atccagccac
     2521 ttctcgtacg caggcgtgcg cccgtgaagg gcgtcctcgt aggcggactg gagctcgcgg
     2581 gtgacctcgc ccggcgcgcc gatctcgatg tcgtcgatct cgcggaccgg caccagctcg
     2641 gcggccgtgc cgaccatgaa cacctcgtcg gccagggtca actcggcgcg ggcgatgtcg
     2701 cgctccacga cggtgtggcc gtggtcggtc gcgatctcga tcagcgactt gcggctgatg
     2761 ccgtccagga tgctggcggt ctgcggcggg gtggcgatct ggccgtcctt gaccacgaag
     2821 atgttctcgc ccgagccctc gcagaccatg ccgcgctcgt ccaggaggat ggcctcctcg
     2881 tagccgccgc gcttggactc gatcttggcc agcaccgagt tcaggtactg cccgccggcc
     2941 ttcgagtgcg ggaccagcga ctgggggctg atgcggcgcc aggacgagac cttggcgcgg
     3001 atgccgttct gcttgccctc gtcgcccagg taggcgcccc actcgaacac ggcgatcgac
     3061 acctcgaccg ggttgtcctc ggggtccagg cccatcgggc cgtggccgcg gaacaccagc
     3121 gggcggatgt aacaggagga gaagccgctg cgcgagatcg actccagcgt ggcctcgcgg
     3181 atctgctcgc gcgagaacgg caggtccatg tagtagagct tggccgactc ctccaggcgc
     3241 ttgatgtggt ccgcgtggcg gaacaccgcg gtgcccttgg tggtgtcgta ggcgcggacg
     3301 ccctcgaaca ccgaggtgcc gtagtggagg gcgtggctca gtacgtgtac cttcgcgtcc
     3361 tcccaggcga cgaactcacc gttcatccag atctggtcgg cgttgttcac gtacgaaccc
     3421 tcccttctag aggtgctgga ggacggcctt cgtggcgtcc tcggtggtgt gggatccccc
     3481 caggtcggcc gtccggatgc cgtccccgag tgctccgtcg acggccgatt ctacggcagc
     3541 ggcggcctcg ttcatagcca gtccgtggcg cagcatcagg gccaccgagc tgaacatggc
     3601 cagcgggttg gccttgccgg tgccggcgat gtccggcgcg gagccgtgga cgggctcgaa
     3661 caggccgggc ccctcgcccc cgatggaggc gctgggcagc atgcccagcg agccggtgag
     3721 catggccgcc tcgtcgctga ggatgtcgcc gaacaggttc tcggtgacga tcacgtcgaa
     3781 gtccgacggg ttggagacca gctgcatcgc ggcgttgtcc accagcaggt gctccagcgg
     3841 cacgtcgggg aactcctgct cgtgcacgcg ggtcacgacc tcgcgccaca gccgtgaggt
     3901 ctcgagcacg ttggccttgt ccacgctggt gaccttggtg cgggcggact cgaagcccac
     3961 gcgggcgatg cgctcgacct cggccacgct gtacgcgcag tcgtcgtggg cggtgtcgcc
     4021 gtcgcggccg gagtcgccga agtagatgcc gccggtgagc tcgcgcacca ccagcaggtc
     4081 ggtgccctgg atgcgctcgg gcttgagcgg gctggcgtgc agcagcgccg ggctgggccg
     4141 gacgggacgc aggttggcga agaggcccag gcccttgcga agccccagca ggccctgctc
     4201 ggggcgcggg gccgacgggt cggccgcgac ggcggcatcc cacttcgggc ctccgacggc
     4261 ggcgagcaga acggcgtcgg cctgcttgca cgcggcgagg acctcgtcgg tgagggcggt
     4321 gc
//
LOCUS       DASUPW010000519         3710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1782689, whole genome shotgun sequence.
ACCESSION   DASUPW010000519 DASUPW010000000
VERSION     DASUPW010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1782689"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1382)
                     /gene="nhaA"
                     /locus_tag="VFY44_11670"
     CDS             complement(<1..1382)
                     /gene="nhaA"
                     /locus_tag="VFY44_11670"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEX5911142.1"
                     /translation="MSESPHTPQPVTPWEETTSRSVREFLRTESGSAGLLLAATLVAL
                     VWANSPLSGSYEDLWGTHLRIQLGGAVIDEDLGHWVNDALMAVFFYLVGLEIRRELSL
                     GELRDRRAAAIPVIAALAGMAVPALLYVAVNAGGDGARGWGIVMATDIAFVLGVVALL
                     GSRCPPGVRLFLLTLAIVDDIGAVAVIAAFYSSGIDLVALGGAAAVLGLVVVGRRLTT
                     WRGPAYVVAGIVLWLLTYASGVHPTIAAVALGLVTAVHPPRRETIARAASLGRVFRRD
                     LSAEGGRAAALEVSSAVSPNERFQQAIHPWSSYLVVPLFALANAGVSLSGDALSRAVS
                     SPITLGVILGLVVGKGLGVSLVAGGAVRAGLGRLPEGLRDSHLVSASALAGIGFTVSL
                     FVTDLAFESEALRDEAKVGVLAASALAAALGWALFRTIDARAPASTREAHLDPPAART
                     DHVRGPADAP"
     gene            complement(1452..2333)
                     /locus_tag="VFY44_11675"
     CDS             complement(1452..2333)
                     /locus_tag="VFY44_11675"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5911143.1"
                     /translation="MELRQLRYLVTLAEEQHFTRAAKRLQIAQPGLSQQIRKLEDELR
                     TPLVDRTTRHVSLTDAGERVAARARSIMGELEAMQSELDGLRGLRTGRVVAGVTPTPG
                     PLGVATLLGRFRAANPEIDLVLREGFSASLAEALRADHIDCAFTTWLSREQRRGLASQ
                     EVASEPLVAVLPHGHPMARRERLSLKDLAGEDFVLFSEGATIRGLVNRTSERLGFRPH
                     VSFEAGETSRIRSIVAEGLGVSVLPRSDALTPGPPVSVAALTDPDMVHRVFVVHRAGR
                     RHGPAVRAFLDLVQEAA"
     gene            complement(2466..>3710)
                     /locus_tag="VFY44_11680"
     CDS             complement(2466..>3710)
                     /locus_tag="VFY44_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911144.1"
                     /translation="LALTRRPARTPLAAARGIALVALGILVLLRPEDALRVVAYAGGA
                     GVVYLGSSELLRVLGSSRRAERARPVRRVAAVAGVAVLTALVATTTAAFVLDRGEQPA
                     AVAASPGDGCNGMRELCGRRVNNVLFPGTHNAMSAADVPGWALTNQRRSIPRQLKDGI
                     RLFLLDPHYGRKGQSGRVLTDFAGEGRDRNKVARELDPPALAAARRLGPSLTRGRPGP
                     RDIWLCHTLCELGATRFTETLTDMRRFLAREPQNVVVLFLENYVTDADMEAAFRKTGT
                     ADMALELDRGEPLPTLGEMIRADKRLLVFTENRTEGVPWLNYGFEWVQDTPLKAERPQ
                     DLNCRPSRGTEDSPILMLNHWIDRFPPPLSGNRKILRERFLARQIRRCERERKMPAAF
                     VAVDYYDQGTLVNVARRINRER"
BASE COUNT          521 a         1392 c         1333 g          464 t
ORIGIN      
        1 tgtggcgcgt ccgcggggcc ccgcacgtga tcggtgcggg cggccggcgg gtccaggtgc
       61 gcctcgcggg tagacgcggg agcacgggcg tcgatggtgc ggaacagcgc ccagcccaat
      121 gcggcggcca gagccgaggc ggccaggacg cccaccttgg cctcgtcgcg cagcgcctcg
      181 gactcgaatg ccaggtcggt cacgaacagc gagacggtga agccgatgcc ggccagggcc
      241 gaggccgaca ccaggtggct gtcgcgcagg ccctcgggca accggcccag cccggcgcgc
      301 accgcgccgc ccgccaccaa cgagacgccc agccccttgc ccacgaccag ccccaggatc
      361 acgcccagcg tgatcggtga gctgaccgcg cgggagagcg cgtcgccgct caggctgacg
      421 ccggcgttgg ccaacgcgaa cagcggcacc acgaggtagg agctccacgg gtgtatcgcc
      481 tgctggaagc gttcgttggg tgacaccgcc gacgacacct cgagcgcggc ggcgcgcccg
      541 ccctccgcgg acaggtcgcg ccggaacacg cgccccaggc tcgccgcgcg ggcgatggtc
      601 tcccgccgcg gcggatggac cgcggtgacc aggcccagcg caaccgccgc gatcgtgggg
      661 tgcacgcccg aggcgtaggt cagcagccac agcacgatgc ccgccaccac gtacgccggg
      721 ccgcgccacg tggtcaggcg cctcccgacc acgacgaggc ccaggaccgc cgccgcgccg
      781 cccagcgcca cgagatcgat gccgctggag tagaaggcgg cgatcaccgc caccgcgccg
      841 atgtcgtcga cgatggccag cgtgagcagg aagagccgca ccccgggcgg gcagcgcgag
      901 ccgagcagcg ccacgacgcc cagcacgaag gcgatgtcgg tggccatgac gatgccccag
      961 cctcgggccc cgtcgccgcc ggcgttcacg gcgacgtaca ggagcgccgg gaccgccatg
     1021 cccgccaacg ccgcgatcac cgggatcgcc gccgcccgcc gatcgcgcag ctcacccagc
     1081 gaaagctcgc gtcggatctc cagccccacg aggtagaaga agacggccat cagggcgtcg
     1141 ttgacccaat ggcccaggtc ctcgtcgatg acggccccgc cgagctggat cctcaggtgg
     1201 gtgccccaga ggtcctcgta ggagccggac agcggcgaat tggcccacac cagcgccacc
     1261 agcgtggccg ccagcagcag tcccgcgctg ccgctctcgg ttcgcaggaa ctcgcgcacc
     1321 gagcggctgg tggtctcctc ccatggagtg accggctgcg gggtgtgggg ggattcgctc
     1381 acggggaaaa tgggtcggcg aggtgtgtcg ccgaccagtc ttcccggcac acctgacggg
     1441 cagcgtactg cttaggccgc ctcctgcacc aggtccagga aggcgcgcac cgcggggccg
     1501 tggcgtcgcc ccgctcggtg gaccacgaac acccggtgga ccatgtccgg gtcggtcagg
     1561 gccgccaccg acaccggcgg ccccggggtc agcgcgtccg agcgcggcag gacgctcacg
     1621 cccagcccct cggccacgat gctgcggatg cgtgaggtct cgcccgcctc gaacgacacg
     1681 tgcgggcgga agcccagccg ctcggacgtg cggttgacca gcccgcggat cgtggccccc
     1741 tcgctgaaca gcacgaagtc ctcaccggcc aggtccttca gcgacagccg ctcgcggcgc
     1801 gccatcgggt ggccgtgggg caggaccgcg accaggggct cactggccac ctcctggctg
     1861 gccagcccgc gccgctgctc gcgcgagagc caggtggtga aggcgcagtc gatgtggtcg
     1921 gcccgcagcg cctcggccag ggacgcgctg aagccctcac gcagcaccag gtcgatctcg
     1981 gggttcgccg cccggaagcg ccccagcagc gtggccaccc ccagagggcc cggggtgggc
     2041 gtgacccccg ccaccacccg gccggtgcgc aggccgcgca gcccgtccag ctcggactgc
     2101 atggcctcga gctcgcccat gatgctgcgg gcccgcgccg ccacccgctc gccggcgtcg
     2161 gtcagggaca cgtgccgggt ggtgcggtcg accagcggcg tgcgcagctc gtcctcgagc
     2221 ttgcggatct gctgcgacag gccgggctgg gcgatctgca gccgcttggc cgcgcgcgtg
     2281 aagtgctgct cctccgccag cgtgaccagg tatctgagct ggcggagctc catagcctgt
     2341 gcttcttaat atcagatgca ctcggtcttg gacttctgaa tggagctggt gcatattgca
     2401 ggggcatcac tcctccctca gcgggtcgcg cgggtcactc cgccggcccg ctttttcgtt
     2461 ccgggtcagc gctcgcggtt gatgcgccgg gccacgttca ccagcgtgcc ctggtcgtag
     2521 tagtccaccg ccacgaatgc cgcgggcatc ttgcgctcgc gctcgcagcg ccggatctgc
     2581 cgggccagga agcgctcgcg caggatcttc cggttgccgc tcagcggcgg cgggaagcgg
     2641 tcgatccagt ggttgagcat caggatcggg ctgtcctcgg tcccgcgcga ggggcggcag
     2701 ttcaggtcct gggggcgctc ggccttcagc ggcgtgtcct gcacccactc gaagccgtag
     2761 ttcagccacg gcacgccctc ggtccggttc tcggtgaaca ccagcagccg cttgtccgcg
     2821 cggatcatct cccccagcgt gggcagcggc tcgccccggt ccagctccag cgccatgtcc
     2881 gcggtcccgg tcttgcggaa cgccgcctcc atgtccgcgt cggtcacgta gttctccagg
     2941 aacaggacca ccacgttctg gggctcgcgg gccaggaacc ggcgcatgtc cgtgagcgtc
     3001 tcggtgaagc gggtcgcgcc cagctcgcag agcgtgtggc acagccagat gtcacgcgga
     3061 ccgggcctgc cgcgcgtgag cgaggggccc aggcggcggg ccgcggccag cgccggcggg
     3121 tcaagctcgc gcgcgacctt gttgcggtcg cggccctcgc cggcgaagtc ggtgagcacc
     3181 cgcccgctct gccccttgcg gccgtagtgc gggtccagca ggaacagccg gatgccgtcc
     3241 ttgagctggc gcggtatcga gcgccgctgg ttggtcagcg cccacccggg cacgtcggcg
     3301 gccgacatgg cgttgtgcgt gccggggaag agcacgttgt tcacgcgccg gccgcacagc
     3361 tcgcgcatgc cgttgcagcc gtcgccgggg ctggcggcca ccgccgccgg ctgctcgccg
     3421 cggtccagca cgaacgccgc cgtcgtggtg gccacgagcg ccgtcagcac ggccacgccg
     3481 gccaccgccg cgacgcgccg gaccgggcgc gcccgctcgg cccggcggga gctgcccagc
     3541 acgcgcagca gctccgagct gcccaggtac accacgcccg ccccgcccgc gtaggccacg
     3601 acccgcagcg cgtcctcggg ccgcagcagc accaggatgc cgagggcgac gagcgcgatg
     3661 ccgcgcgcgg cggccagggg cgtgcgcgcg ggccggcgcg tcagcgccag
//
LOCUS       DASUPW010000520         3039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1366277, whole genome shotgun sequence.
ACCESSION   DASUPW010000520 DASUPW010000000
VERSION     DASUPW010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366277"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VFY44_11685"
     CDS             complement(<1..466)
                     /locus_tag="VFY44_11685"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5911145.1"
                     /translation="MADTETHTDPHYDRRWLILGVIGIAQLMVVLDMTIVNIALPSIQ
                     NDLGFSDDQRQWLITAYALAFGSLLLLGGRISDLFGRKRAFVTGLLGFAAASAIGGAA
                     QSFGVLVGARALQGVFGALLAPAALSLLATIFTTPSERGKAFGVFGAIAGAGG"
     gene            563..1204
                     /locus_tag="VFY44_11690"
     CDS             563..1204
                     /locus_tag="VFY44_11690"
                     /inference="COORDINATES: protein motif:HMM:NF044515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5911146.1"
                     /translation="MTNKTLRRDAELNRQRLMESAAELFRERGLDVTLDDVAEHAGVG
                     VGTAYRRFADKDELIDALLESRIDEMVAMAERGLEDPDPWDGLVSYIVGALELQVRDR
                     GLKDVLFAPGRGKKRVAAAKGRMGPAVTALADRARAAGVVREDFQPSDIPLIYLMLGT
                     VVDFSRGFEDGLYRRYLAILLEGIRAGGQPPELPVPALELETFQAAMARFKSR"
     gene            complement(1201..1836)
                     /locus_tag="VFY44_11695"
     CDS             complement(1201..1836)
                     /locus_tag="VFY44_11695"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5911147.1"
                     /translation="MTTAMLDSPVEGLEATAPQSLSFAPTTQVRAFLLRRPLGDLLVY
                     ANDKVDRSLMADRQYLGHWHEAMVAGRDSGLPVFVHDADRAEVTPVMPVRGGFTLRHK
                     LASDFEVIPMPGHTPGSTAYLWDSGTHRLLFTADSLYLGGDGAWRAAVLESSDRERYI
                     RSLELLRGLDFDVLVPWAAPADGPYVDVVEPAEARRRLDEVIARLRAGENS"
     gene            1918..>3039
                     /locus_tag="VFY44_11700"
     CDS             1918..>3039
                     /locus_tag="VFY44_11700"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX5911148.1"
                     /translation="MERPRAGTWFATAAIAVAVGLVLVVVLLLTGALFDSADDLVTVF
                     LAGVAVTALVGWWQDKAERAARDAAEASERERADRERHEEHMREVKTRTRLERAHIAE
                     KQWARELRSQVARLHKQQGALGNTGDVREMVLQTAVKLVDADRGLLLSRRDDDNDGDF
                     DMACQVGFESDATHSAVAQEYAGKVLRRDETVREDDSRQLRGEGRNPADDEIDNLLAI
                     PIFIQDDFEGVVLCANREGGFEDLDDDVLLALGDHAGAVLENGKLQGEVRSTYMAIVR
                     MLAEAIEAKDPATRLHSEDVAEYVRGVADGLGLEARHREELLIASLLHDVGKIGVSER
                     ILLKPGALTPDERAAIELHSRIGYHLVRQVPALHGIADAV"
BASE COUNT          468 a         1024 c         1147 g          400 t
ORIGIN      
        1 cgccgccggc gcccgcgatg gcgccgaaga cgccgaacgc cttgccgcgc tcggacgggg
       61 tggtgaagat cgtggccagc agcgacagcg cggccggcgc gagcagcgcg ccgaagacgc
      121 cctgcagcgc ccgtgcgccg acgagcacgc cgaagctctg cgcggcgccg ccgatggcgg
      181 acgcggcggc gaagcccagc aggccggtca cgaacgcgcg cttgcgcccg aacaggtcgc
      241 tgatccgccc gcccagcaac agcagcgagc cgaaggcgag cgcgtaggcg gtgatcagcc
      301 actggcgctg gtcgtcggag aagccgaggt cgttctggat cgacggcagc gcgatgttca
      361 cgatcgtcat gtccagaacc accatcagct gggcgatgcc gatgacgccg aggatgagcc
      421 aacggcggtc gtagtgggga tcggtgtgcg tctcggtgtc ggccattgtg gatcctttac
      481 atcaagcgga ggcagaagct ccggttaccg tagcaaagaa gtggaggtat gtcctccaca
      541 tctgcactac ccttcgttac cgatgacgaa caagacgctc agacgtgacg ccgaactgaa
      601 ccgccagcgc ctcatggagt ccgccgcgga gctgttccgc gagcggggcc tggacgtcac
      661 gctggacgac gtggccgagc acgcgggcgt cggggtcggc accgcctacc gccggttcgc
      721 cgacaaggac gagctgatcg acgcgctgct ggagagccgc atcgacgaga tggtggccat
      781 ggccgagcgc ggcctggagg accccgaccc gtgggacggc ctggtgagct acatcgtcgg
      841 cgcgctggag ctgcaggtgc gcgaccgggg gctgaaggac gtgctgttcg cgcccggccg
      901 cggcaagaag cgcgtggcgg cggccaaggg ccggatgggc cccgcggtca cggcgctggc
      961 cgaccgggcc cgcgcggccg gcgtggtgcg cgaggacttc cagcccagcg acatcccgct
     1021 gatctacctg atgctgggca ccgtggtgga cttcagccgc gggttcgagg atgggctgta
     1081 ccgccgctat ttggccatcc tcctggaggg catccgcgcg ggcgggcagc cgcccgagct
     1141 gccggtcccg gccctggagc tggagacgtt ccaggcggcc atggccaggt tcaagagccg
     1201 ctaggagttc tcgccggcgc gcagccgcgc gatgacctcg tccaggcgcc gccgggcctc
     1261 ggccggctcg accacgtcca cgtagggccc gtccgccgga gcggcccagg gcaccaggac
     1321 gtcgaagtcc agaccgcgca gcagctcgag gctgcggatg taccgctcgc ggtcgctgga
     1381 ctccagcacc gccgcgcgcc aggcgccgtc gccgcccagg tagagcgagt cggcggtgaa
     1441 cagcaggcgg tgggtcccgc tgtcccacag gtaggcggtc gaccccggcg tgtgcccggg
     1501 catggggatc acctcgaagt cgctggccag cttgtggcgc agggtgaagc cgccgcgcac
     1561 gggcatcacg ggcgtgacct cggcgcggtc ggcgtcgtgc acgaacacgg gcaggccgct
     1621 gtcgcgcccg gccaccatcg cctcgtgcca gtggccgagg tactggcggt cggccatcag
     1681 gctgcggtcg accttgtcgt tggcgtacac gagcaggtcg ccgagcgggc gccgcagcag
     1741 gaaggcgcgc acctgggtgg tcggggcgaa ggacaggctc tggggcgcgg ttgcctccag
     1801 gccctccacg gggctgtcca gcatggcggt ggtcatgaca ggcatggtgc atcttcaagt
     1861 gcgcttgaag tcaagggcac ttgttcgccg aagcgattcc ggagtagctt tgcgctgatg
     1921 gaacgacccc gcgccggaac ctggttcgcc actgccgcca tcgccgtggc cgtcgggctg
     1981 gtgctggtcg tggtcctgct gctgaccggc gccctcttcg actccgcgga cgacctggtg
     2041 accgtgttcc tggccggcgt ggccgtgacg gcgctggtgg gctggtggca ggacaaggcc
     2101 gagcgcgccg cgcgcgacgc cgcggaggcc tccgagcgcg agcgggccga ccgcgagcgc
     2161 cacgaagagc acatgcgcga ggtgaagacg cgcacgcggc tggagcgcgc ccacatcgcg
     2221 gagaagcagt gggcgcgcga gctgcgcagc caggtggccc ggctgcacaa gcagcagggc
     2281 gcgctgggca acaccggcga cgtgcgcgag atggtgctgc agaccgccgt gaagctggtc
     2341 gacgccgacc gcggcctgtt gctctcgcgc cgcgacgacg acaacgacgg cgacttcgac
     2401 atggcctgcc aggtcgggtt cgagtccgac gccacccaca gcgcggtcgc ccaggagtac
     2461 gccggcaagg tcctgcgccg agacgagacg gtgcgcgagg acgactcgcg ccagctgcgc
     2521 ggcgaggggc gcaacccggc ggacgacgag atcgacaacc tgctggccat cccgatcttc
     2581 atccaggacg acttcgaggg cgtcgtgctg tgcgccaacc gcgagggcgg gttcgaggac
     2641 ctggacgacg acgtgctgct ggccctggga gaccacgccg gcgcggtgct ggagaacggc
     2701 aagctgcagg gcgaggtgcg ctcgacctac atggcgatcg tgcggatgct ggccgaggcg
     2761 atcgaggcca aggacccggc cacccgcctg cactccgagg acgtggccga gtacgtgcgg
     2821 ggcgtggccg acgggctggg cctggaggcc cgtcaccgcg aggagctgct gatcgcctcg
     2881 ctgctgcacg acgtgggcaa gatcggcgtg tccgagcgca tcctgctcaa gccgggcgcg
     2941 ctgacgcccg acgagcgcgc cgcgatcgag ctgcactcgc ggatcggcta ccacctggtc
     3001 cgccaggtgc ccgccctgca cgggatcgcc gacgcggtg
//
LOCUS       DASUPW010000521         4484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1643867, whole genome shotgun sequence.
ACCESSION   DASUPW010000521 DASUPW010000000
VERSION     DASUPW010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1643867"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1321)
                     /locus_tag="VFY44_11705"
     CDS             complement(<1..1321)
                     /locus_tag="VFY44_11705"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5911149.1"
                     /translation="MAPSSNGVRLEAGHVTATDAETLQRAMDSTSNGVVIASASGSDY
                     AIVYMNAGFERITGYGCDEVVGRDCRFLQGEATDPTAVAELSRAVRNGAECRLTILNY
                     RKDGEPFWNELFLSPIRDAGGDVVQYVGIQNDVSERQEAQEQVQHLAYHDALTGLGNR
                     AKLERDLVSALEAAAAAGESVALLYLDLDDFKGVNDSLGHVSGDELLRQAADRTQRAC
                     RPGDLLIRQGGDEFLVLLTGLSEEAEKTASAVAARVAQTMSDPFDLGGHSVTIGASVG
                     ISLFPRDAADAETLLTHADTAMYVAKGAGRGRVALYAGEHQTAPHRRGQRTRETPESV
                     QEIIAAGAVRPVYQPIVELRNGAIAGYEALSRGPVGSPLERPDLLFEEARKHGLTAQL
                     DWLCRAEALRGAMEAGLSSPLSLFVNAEPVTLDSPVPEPARAVWERTR"
     gene            complement(1393..1539)
                     /locus_tag="VFY44_11710"
     CDS             complement(1393..1539)
                     /locus_tag="VFY44_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911150.1"
                     /translation="MQAFDGKPKAEDTKYEEAEEVGLEGLAPKKGNEDAPPPEETHTP
                     HDDA"
     gene            complement(1542..2033)
                     /locus_tag="VFY44_11715"
     CDS             complement(1542..2033)
                     /locus_tag="VFY44_11715"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5911151.1"
                     /translation="MGAVSKYVRIAAPPQQVYDLWRDPTHFPDFMPDVQSVTANGDSW
                     HWKVDSVAGTSIEWDSEIVEDLPGERLAWKSVGGKVENSGAVRFDDRDGQTDVEFAME
                     FDPPAGVAGEVVAKLFKDPEDQVQRSLDAFKQLVEKQAQPRDDARTEVDAAEAKPPHT
                     EAA"
     gene            2171..2656
                     /locus_tag="VFY44_11720"
     CDS             2171..2656
                     /locus_tag="VFY44_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911152.1"
                     /translation="MKRCLIIVVALLALSANAWAASAAAPALKTGTWKGQSNQSDSVR
                     FTVKKDGGDYVAGSTRVSTVVGCDAPNPRDSTYSEEIKRSRITVKLSASGDTLRGSRT
                     VTRKTGGGKTTETVKVAMKFTSARRAKVVVNASANTKGKNVNPNAAKCGGRVELTAKP
                     Q"
     gene            complement(2678..3058)
                     /locus_tag="VFY44_11725"
     CDS             complement(2678..3058)
                     /locus_tag="VFY44_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5911153.1"
                     /translation="MPEWTLKNFADIEDRSPDDVDMEWHFARKELGFEQIGVSRFTFE
                     PDVRFPFAHRHREQEEAYVVVAGSGRAKLGDDIVELTQWDVVRVPPSVGRQFEAGPDG
                     LELICVGGPRPEGGDGEPVEGFWD"
     gene            3141..3761
                     /locus_tag="VFY44_11730"
     CDS             3141..3761
                     /locus_tag="VFY44_11730"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX5911154.1"
                     /translation="MKLTVTTFQTLDGVMQAPGGAEEDTSGGFDQGGWVAPHFDAEVN
                     EFMTGVFERPEAFLLGRRTYEIFAAHWPEAADPDDPISNGLNTLPKHVASTTLTSVEW
                     ENSSLLEGDVVQAITDLKAREGGELQVHGSANLIQTLNEHGLVDEYNVLTFPVIVGGG
                     KRLFEDGAKPVGLESVWSTKTPSGVTIDVWRTAGAPQRAEAAEIER"
     gene            3835..>4484
                     /locus_tag="VFY44_11735"
     CDS             3835..>4484
                     /locus_tag="VFY44_11735"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HEX5911155.1"
                     /translation="MSIRPTVSTRRFAVASLSAGALAAIALPGNRVGLGMVLVAALVL
                     VAVALCGALRRTWDVGAGVALAAALAAMAVVRDASWLVAFDLIAATAVLVLTAVRARS
                     WRAMANAVFVWTAELVPAPAIVAWCTGIRPAPRLSGRLAPLARGLALGLALVVVFGAL
                     FASADQAFAHLTGQVLDPEIDLGSATARIGAGLVAVSVCGAVWLTALATDGRPEPAR"
BASE COUNT          594 a         1556 c         1659 g          675 t
ORIGIN      
        1 atcgcgtgcg ctcccacacc gcgcgggccg gctcggggac gggcgagtcg agggtgaccg
       61 gctcggcgtt gacgaacagg ctcagcggcg acgagagacc ggcttccatc gcaccgcgga
      121 gcgcctcggc gcggcagagc caatccagct gggccgtgag cccgtgcttg cgcgcctcct
      181 cgaagagcag gtccggacgt tccagcggtg agccgaccgg accgcggctg agtgcctcgt
      241 agcccgcgat ggcgccgttg cgcaactcca cgatcggctg gtagacgggt cgcacggccc
      301 cggccgcgat gatctcctgc accgactccg gggtctcccg ggtgcgctgg ccacgccggt
      361 gcggcgcggt ctggtgctca cccgcgtaca gcgccacccg gcccctcccg gcgcctttgg
      421 ccacgtacat cgccgtgtcg gcgtgggtga gcagggtctc ggcgtcggct gcgtcgcgcg
      481 ggaacagcga gatgccgacg ctggcgccga tggtgacgct gtggccgccc agatcgaacg
      541 ggtcgctcat ggtctgggcc acccgggcgg ccaccgcgga cgccgtcttc tcggcctcct
      601 cggacaggcc cgtgagcagg accaggaact cgtcgccgcc ctggcggatc agcaggtcgc
      661 ccgggcgaca ggcgcgctgt gtgcgatcgg cggcctggcg cagcagctcg tcgccgctca
      721 cgtggcccag cgagtcgttg acgcccttga agtcgtccag gtccaggtac agcagggcca
      781 ccgactcgcc ggccgcggcg gcggcctcga gcgcggagac caggtcgcgc tcgagcttgg
      841 cgcggttgcc caggccggtg agcgcatcgt ggtaggccag gtgctggacc tgctcctggg
      901 cctcctggcg ctcggatacg tcgttctgga tgcctacgta ctggaccacg tcgccgccgg
      961 cgtcccggat cggcgacagg aagagctcgt tccagaacgg ctcaccatct ttgcggtagt
     1021 tcaggatggt gagccggcac tcggcgccgt tgcgtaccgc gcggctcagc tcggcgacgg
     1081 ccgtggggtc ggtcgcttcg ccctgcagga agcggcagtc gcgcccgacc acctcgtcgc
     1141 agccgtagcc cgtgatgcgc tcgaagccgg cgttcatgta gacgatcgcg tagtcgcttc
     1201 ccgacgcgct ggcgatgacg accccgttgg aggtcgagtc catcgcccgc tggagggtct
     1261 cggcgtcggt cgcggtgacg tgccccgcct cgagccggac gccgttggag gagggggcca
     1321 tcgggtttgt atcgacgccc gggcgagcgg cttgaatcag ctggcgtgga gccgctcgcc
     1381 cgtcttcgcg agctaggcgt cgtcgtgggg cgtgtgcgtc tcctcgggag gcggcgcgtc
     1441 ctcgttgccc ttcttgggcg cgaggccctc gaggccgacc tcttcggcct cctcgtactt
     1501 ggtgtcctcg gccttgggct tgccgtcgaa ggcctgcatg gtcaggccgc ctcggtgtgg
     1561 ggcggcttgg cctcggcggc gtcgacctcg gtgcgggcgt cgtcgcgcgg ctgggcctgc
     1621 ttctcgacca gctgcttgaa cgcgtccagc gagcgctgga cctggtcctc ggggtccttg
     1681 aagagcttgg ccacgacctc gccggcgacg ccggcgggcg ggtcgaactc catggcgaac
     1741 tcgacgtcgg tctgaccgtc gcggtcgtcg aagcgcacgg ctccggagtt ctcgaccttg
     1801 ccgccgacgg acttccacgc caggcgctcg ccgggcaggt cctcgacgat ctcggagtcc
     1861 cactcgatgg aggtgccggc gaccgagtcg accttccagt gccaggagtc accgttggcg
     1921 gtgacggact ggacgtcggg catgaagtcg gggaagtggg tggggtcgcg ccagaggtcg
     1981 tagacctgct gcggcggtgc tgcgatgcga acgtatttgg aaaccgctcc catgatgtgt
     2041 cctctcattg ggttcgagag gtgtacgggt gccgcggggc gcaagatcta gcccctcgcc
     2101 ggccgacttg cgcggatcgg cattccgtgt ttggttgatg cggtccgccg tcccccctgt
     2161 aggaggcctc ttgaagcgct gcctgatcat cgtcgtcgcc ctgctcgccc tgagcgcgaa
     2221 cgcctgggcc gcctccgcgg ctgcgccggc cctgaagacg ggcacctgga agggccagag
     2281 caaccagagc gactcggtgc gcttcacggt gaagaaggac ggcggcgact acgtggccgg
     2341 ctccacccgc gtgtcgacgg tggtgggatg cgacgcgccc aaccccaggg acagcaccta
     2401 cagcgaggag atcaagcgga gccggatcac ggtgaagctc agcgcgtccg gcgacaccct
     2461 gcgcggctcg cgcacggtga cgcgcaagac cggtggcggc aagaccacgg agaccgtgaa
     2521 ggtcgcgatg aagttcacca gcgcgcgccg ggcgaaggtg gtcgtgaacg ccagcgccaa
     2581 caccaagggc aagaacgtca acccgaacgc cgccaagtgc ggcggccgcg tggagctgac
     2641 cgccaagccg cagtagcggc cgcgccggcc cacccgctca gtcccagaag ccctcgaccg
     2701 gctcgccgtc cccgccctcg gggcgcgggc cgccgacgca gatgagctcg agcccgtcgg
     2761 ggccggcctc gaactggcgg ccgacggagg gcgggacccg gacgacgtcc cactgcgtca
     2821 gctcgacgat gtcgtcgccc agcttcgccc gtcccgagcc ggcgacgacc acgtaggcct
     2881 cctcctgctc gcggtggcgg tgggcgaacg ggaagcgcac gtcgggctcg aaggtgaagc
     2941 ggctgacgcc gatctgctcg aagccgagct ccttgcgggc gaagtgccac tccatgtcca
     3001 cgtcgtcggg gctgcggtcc tcgatgtcgg cgaagttctt cagggtccat tcgggcatgg
     3061 ttcgagtatc ccggcccgga tgtcgaaagc gcggccgccc gttcgacgca cccgcagaac
     3121 cccgaacgag aggacccgac atgaagctca ccgtgaccac attccagacc ctggacggcg
     3181 tgatgcaggc ccccggcggc gccgaggagg acaccagcgg cggcttcgac cagggcggct
     3241 gggtggcccc ccacttcgac gccgaggtga acgagttcat gaccggcgtg ttcgagcgcc
     3301 ccgaggcgtt cctgctgggc cggaggacgt acgagatctt cgccgcgcac tggcccgagg
     3361 cggccgatcc cgacgacccg atctcgaacg gcctgaacac gctgcccaag cacgtggcgt
     3421 cgaccacgct gaccagcgtg gagtgggaga actcgtcgct gctggagggc gacgtcgtcc
     3481 aggcgatcac cgacctcaag gcgcgcgagg gcggcgagct gcaggtgcac ggcagcgcga
     3541 acctgatcca gacgctgaac gagcacggcc tggtggacga gtacaacgtg ctcacgttcc
     3601 cggtcatcgt cggcggcggg aagcggctgt tcgaggacgg ggcgaagccg gtcggcctgg
     3661 agtcggtgtg gtcgacgaag acgccgtcgg gcgtgaccat cgacgtgtgg cgcaccgccg
     3721 gggccccgca gcgggccgag gcggccgaga tcgagcgcta ggccgggcgc gcctgcacga
     3781 agcctccttg gagaccaccc gtggcctcca cgggggcttc gtagcgtcgc gcccatgtcg
     3841 atccgaccga cggtctccac gcggcgcttc gccgtcgcct ccctgtccgc cggcgctctg
     3901 gcggcgatcg cgctcccggg caaccgggtc ggcctgggca tggtcctggt ggccgccctg
     3961 gtgctggtcg ccgtggcgct gtgcggcgcg ctgaggcgaa cgtgggacgt gggcgccggg
     4021 gtcgcgctgg ccgccgcgct ggcggcaatg gcggtcgtgc gtgacgcgtc gtggctggtg
     4081 gcgttcgacc tgatcgccgc caccgcggtg ctggtgctga ccgcggtgcg cgcccgcagc
     4141 tggcgggcga tggccaacgc cgtcttcgtc tggaccgccg agctggtgcc cgcgccggcg
     4201 atcgtcgcct ggtgcaccgg catccgcccg gcgccgcggc tgagcggccg gctcgccccg
     4261 ctggcgcgcg gcctggccct cgggctggcc ctggtggtgg tcttcggcgc gctgttcgcc
     4321 tccgccgacc aggcgttcgc gcacctgacc ggccaggtcc tggaccccga gatcgacctg
     4381 ggcagcgcga cggcgcggat cggcgccggg ctggtggccg tgtcggtctg cggggccgtg
     4441 tggctgaccg cgttggccac cgacggccgc ccggagccgg cccg
//
LOCUS       DASUPW010000522         6133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_813114, whole genome shotgun sequence.
ACCESSION   DASUPW010000522 DASUPW010000000
VERSION     DASUPW010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813114"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..2687)
                     /locus_tag="VFY44_11740"
     CDS             complement(123..2687)
                     /locus_tag="VFY44_11740"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5911156.1"
                     /translation="MIRVAAKALAQRKLRTALTVLAIVVGVGMVSAALVLGDSMKKGA
                     DSLSTSSYRGTDAVVSAKTPFTVSSQTAGATPTIPASVLERVRSVPEVGAAVGDITDQ
                     QVKLAGKDGEPVGDGPYFGVGYDAGAKGAGDLTPFQLRQGRFARGADEVVVDAGTAEK
                     QGWKVGDPVRVSGQGPARRMRISGIARFGDVDSLGTATSAVMDLGAAQSLLGREGRFD
                     NVLVAAAPGVSAAELRRALARELPSAVRVQSAAASDRFALDSLSQVVKWVKVILLVFG
                     GVAILVGAFTIANTMSITLAQRSRELALMRAVGATRGQIRRLVVAEAAVMGAIGSALG
                     IVAGVFIAKGLSSVMAAAAMELPDAGTVFGPSTVVVSLLVGLGVTVLAALGPASRSTR
                     VSPVTAMREGADIPPSRFGRRSVPIAVGMLGLSALLLAAAAFAPGIDAGGRGALIGPG
                     VLLLFLGVALVSPKLARPLASVIGRPLERIGGSAGALARRNAMRDPGRTAATASALMI
                     GLSLVVFVSVVGQGIRQSTTGALEKQVHAQYVVSSTDGWTEIDPAAAAAVRKAPGVRS
                     VSELTQDQARAFGKEAGVNGVDPKTIDSVYGFDVTAGAKPSPASLRGDGALVEKGFAE
                     ENGLKVGSAFTVTSAKGDRLRLKVTGIGDPPKFDPLSLGEVVVSQSTFARSFTNQRDR
                     LAFVSADGDSQAAIDRALAGFPTVQALTAADFADEQMAWVDDALGILYVLLALSVIVS
                     LFGIVNTLVLSVFERRRELGMLRAVGMTRRQVRRMVRHESVITALIGAALGVGVGLAL
                     AGLVTSAFSDQGLAFSVPVGSLIAFAIVAGLAGVLAAVLPARRAARLDPLTALAYQ"
     gene            complement(2684..3466)
                     /locus_tag="VFY44_11745"
     CDS             complement(2684..3466)
                     /locus_tag="VFY44_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5911157.1"
                     /translation="MFTKSIGAPDAPALPDPQLVVPAVAAAGLRRDYGSGESAVHALR
                     GVSLEVPTGQFTAVMGPSGSGKSTLMHLLAGLDTPTAGSVRVGGSELAGMDDDELTRM
                     RRTEIGFVFQAFNLLPMLSVEENVLLPARIAGAKPDAALLEQVIAGLGLADRRRHLPS
                     ELSGGQQQRVAIARAIVGRPAVLFADEPTGNLDSATGAEILGVLREAVDSHGQTTVMV
                     THDAHAAAVADRVVFLADGRIEDDLYGAGEDQILDVMKEMAR"
     gene            complement(3501..3941)
                     /locus_tag="VFY44_11750"
     CDS             complement(3501..3941)
                     /locus_tag="VFY44_11750"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5911158.1"
                     /translation="MAHWTAQTTAQVPPEQVLAVLTDPDAIRTWSPVPFELEGAESRL
                     AAGRTARVNGQLAGVRVGFDVEVHSAGAEGLDLTAHGPVALDVHYELTPTAGGSSVKA
                     SVGLRRARGITGRLVASATGALLSAGALEGAAGRIARAAEATAA"
     gene            4106..5290
                     /locus_tag="VFY44_11755"
     CDS             4106..5290
                     /locus_tag="VFY44_11755"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX5911159.1"
                     /translation="MRHLRQLFSRAREADPRRFDAVLTGVLLAGAMVEIVLLHANGAN
                     RWASIPVTLIALVPLYWRRTHTLEACIAFGVAFGLNVLLDTLFMDSMTSPFLAVGILF
                     YSLGRYTDGWRTWAGVLVIYLPTSTGLALTNTEQPFVLAMLWSVALLAPPLVAGRIMR
                     KRLEMQAELRDLTARLESDRGRREQRAIDSERGRIAEELQTLVANNVSAMVVQAGVVR
                     PALAIPAPTTADTALATIEESGRDALVEMRRLLGVLRRDDAVAELAPQPSLDQALSLV
                     ERTRAEGLPVHLHVDGEPSPLARGVDLAAYRVLQETLDSARQAEGSRKAQVLLAYGDR
                     EVTVAVAVRDDGRGQRLLGEDSLSALRERVGLYGGTLRAGRLKDDHGFRVSARLPLEA
                     DA"
     gene            5287..>6133
                     /locus_tag="VFY44_11760"
     CDS             5287..>6133
                     /locus_tag="VFY44_11760"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain-containing protein"
                     /protein_id="HEX5911160.1"
                     /translation="MIRRIRRLTERDRRVIDIAIAVLMTAAAVAVVLTSPDIEGPLGL
                     NAALYAVVTLALVARRAHVLEATVVSVSAWFVILAFLSAPFYVPFATFSLMLLSYSAG
                     SYATGRRSHAALALVALTILVTCIVETPEDIAFPVAIFGIAPYVGGRILRNHTLLTRE
                     LAEKAELAAHLGDAAERDAVAAERVRVARELHDVLAHELSVMVIQAQGARRMIPRDPE
                     AAAEVADLIGRTGRDALTELRRLFGSVRHGEGEDLSGPPGLGRLDELVGRARAAGLPV
                     ELRTEG"
BASE COUNT          799 a         2284 c         2294 g          756 t
ORIGIN      
        1 ggaccgcggc cggtgcgccg cgggccggaa ggtgcgcgct gacggacata tcccggcccg
       61 cgaggcatga accgcctcgc gggcccgtcc gtgcgccccc gtccccgggg ccggcgggcg
      121 gcctactggt aggccagcgc cgtcagcggg tccagccgtg ccgcgcggcg cgcgggcagc
      181 accgccgcca ggacaccggc caggccggcg acgatggcga acgcgatcag cgagccgacg
      241 gggacggaga acgccaggcc ctggtcgctg aaggcgctgg tgaccaggcc cgccagcgcc
      301 aggccgacgc cgacgccgag ggcggcgccg atgagggcgg tgatcacgct ctcgtggcgc
      361 accatccgcc gcacctggcg ccgggtcatg cccacggccc gcagcatgcc cagctcgcgg
      421 cggcgctcga acaccgacag cacgagcgtg ttgacgatgc cgaacagcga cacgatcacc
      481 gacagcgcca ggagcacgta gaggatgccc agggcgtcgt cgacccaggc catctgctcg
      541 tcggcgaagt cggccgcggt cagggcctgg acggtgggga acccggccag cgccctgtcg
      601 atcgcggcct ggctgtcacc gtcggccgag acgaacgcca gccggtcgcg ctggttggtg
      661 aacgagcgcg cgaacgtgct ctgggacacg accacctcgc caagcgacag cgggtcgaac
      721 ttgggcgggt cgccgatgcc cgtgaccttc agccgcaggc ggtcgccctt ggccgacgtg
      781 accgtgaacg cgctgccgac cttcaggccg ttctcctcgg cgaacccctt ctcgaccagg
      841 gcgccgtccc cgcgcagcga cgcgggcgaa ggcttggcgc cggccgtcac gtcgaacccg
      901 tagaccgagt cgatcgtctt cgggtccacc ccgttcacgc ccgcctcctt gccgaacgcg
      961 cgcgcctggt cctgggtcag ctcggacacc gagcggacgc ccggggcctt gcgcaccgcc
     1021 gcggcggcgg cgggatcgat ctcggtccag ccgtcggtgg aggagaccac gtactgcgcg
     1081 tggacctgct tctccagcgc gccggtcgtg gactggcgga tgccctgccc gaccaccgag
     1141 acgaacacga ccagcgacag gccgatcatc agcgccgagg cggtggccgc ggtgcggccg
     1201 gggtcgcgca tcgcgttgcg ccgcgccagc gcgccggcgc tgccgccgat ccgctccagc
     1261 ggccggccga tgaccgaggc cagcggccgc gccagcttcg gcgagaccag ggcgacgccg
     1321 aggaacagca gcagcacgcc cgggccgatc agggctccgc gcccgccggc gtcgatgccc
     1381 ggcgcgaacg ccgcggcggc cagcaggagc gccgacaggc cgagcatgcc cacggcgatg
     1441 ggcaccgagc ggcggccgaa tcgcgacggc gggatgtccg ccccctcgcg catcgccgtg
     1501 accggcgaca cgcgcgtgga ccgcgacgcc gggccgagcg ccgccaggac ggtcacgccc
     1561 aggcccacga gcagcgagac gacgaccgtc gaggggccga acacggtgcc cgcgtcgggc
     1621 agctccatgg ccgccgcggc catgaccgac gacaggccct tggcgatgaa gacgccggcc
     1681 acgatgccca gtgccgagcc gatggccccc atgaccgcgg cctcggccac gaccaggcgg
     1741 cggatctgcc cgcgcgtggc ccccaccgcg cgcatcagcg ccagctcgcg cgagcgctgg
     1801 gcgagcgtga tggacatggt gttggcgatg gtgaacgcgc cgaccaggat ggccacgccg
     1861 ccgaacacca gcaggatcac cttgacccac ttgaccacct gcgagagcga gtcgagcgcg
     1921 aaccggtcgc tcgcggccgc gctctgcacg cgcaccgccg agggcagctc acgcgcgagc
     1981 gcgcgccgca gctcggccgc ggacacgccc ggcgccgccg cgaccagcac gttgtcgaac
     2041 cggccctcac ggcccagcag ggactgggcg gcgcccaggt ccatgacggc ggaggtggcg
     2101 gtgcccagcg agtcgacgtc cccgaagcgg gcgatgcccg agatgcgcat ccgccgcgcg
     2161 ggcccctggc cggacacccg cacggggtcg ccgaccttcc agccctgctt ctcggccgtg
     2221 ccggcgtcga cgacgacctc gtccgcgccg cgggcgaacc ggccctggcg cagctggaac
     2281 ggggtcaggt cgccggcgcc cttcgcgccg gcgtcgtagc ccacgccgaa gtaggggccg
     2341 tcgcccaccg gctcgccgtc cttgcccgcc agcttgacct gctggtcggt gatgtcgccg
     2401 acagcggcgc ccacctcggg caccgagcgc acgcgctcca gcacgctcgc cgggatcgtg
     2461 ggcgtggcgc ccgcggtctg gctggacacc gtgaacggcg tcttggccga gaccaccgcg
     2521 tcggtgcccc ggtaggagga ggtggacagc gagtcggccc ccttcttcat cgagtcgccg
     2581 agcaccagcg cggcgctgac catgcccacg cccaccacga tcgccaggac cgtgagggcg
     2641 gtgcgcagct tgcgctgcgc cagtgccttg gcggctacgc ggatcatcgg gccatctcct
     2701 tcatgacgtc gaggatctgg tcctcgccgg cgccgtacag gtcgtcctcg atccggccgt
     2761 cggccaggaa gacgacgcgg tcggccacgg ccgccgcgtg cgcgtcgtgg gtgaccatga
     2821 ccgtggtctg gccgtgcgag tccaccgctt cgcgcagcac gccgaggatc tcggcacccg
     2881 tggccgagtc caggttgccg gtgggctcgt cggcgaacaa caccgccggc cggccgacga
     2941 tggcgcgggc gatggccacg cgctgctgct ggccgccgga gagctcggag ggcaggtggc
     3001 ggcggcggtc ggccaggccc agcccggcga tgacctgctc gaggagcgcg gcatcgggct
     3061 tcgcgccggc gatccgagcc gggagcagga cgttctcctc gacgctcagc atcggcagca
     3121 ggttgaaggc ctggaacacg aacccgatct ccgtgcggcg catgcgggtg agctcgtcgt
     3181 cgtccatgcc ggccagctcg ctgccaccga cccggacgct gcccgcggtg ggggtgtcga
     3241 gcccggccag caggtgcatc agcgtcgact tgcccgagcc cgagggcccc atgacggcgg
     3301 tgaactggcc ggtgggcacc tccagcgaca cgccgcgcag cgcgtgcacg gcggactcgc
     3361 ccgagccgta gtcccgtctc aggccggcgg cggccacggc cggcaccacg agctgggggt
     3421 cggggagggc cggcgcgtcc ggggcgccga tggacttcgt gaacatgcgg atctcctctg
     3481 gtgtgcgagt cgggttcatg tcaggccgcc gtggcctcgg ccgcgcgggc gatccgcccc
     3541 gcggcccctt cgagtgctcc ggccgacagg agggcgccgg tggccgaggc caccagccgt
     3601 cctgtgatcc cgcgcgccct gcgcagcccc acggaggcct tgacgctgct gccgccggcg
     3661 gtcggggtga gctcgtagtg cacgtccagc gccaccgggc cgtgggccgt caggtccagt
     3721 ccctcggcgc cggcggagtg cacctcgacg tcgaagccca cgcgcacgcc tgcgagctgg
     3781 ccgttcacgc gcgcggtgcg ccccgcggcc aggcggctct cggcgccctc gagctcgaac
     3841 ggcacgggcg accaggtgcg gatggcgtcc gggtcggtca ggaccgcgag cacctgctcg
     3901 ggcgggacct gggcggtggt ctgtgcggtc cagtgggcca tgacgccaga ctggaccggc
     3961 gcggccctcc tggcatcggc ccaagtgacg atcctgcggg ggcgccgcgg acgaccccgg
     4021 gggcgcgcgg gtcctacctt cggatgacac ccgcctcgtc ggcgcggatg atggacaggc
     4081 gccggaccgg ctgtaccttg gccagatgcg gcacctccgt cagctgttct ctcgcgcgcg
     4141 cgaggccgat ccgcgccgct tcgacgccgt gctcaccggc gtcctgctgg ccggggcgat
     4201 ggtggagatc gtgctgctgc acgccaacgg cgccaaccgg tgggccagca tcccggtcac
     4261 gctgatcgcg ctggtcccgc tctactggcg ccgcacccac acgctcgagg cgtgcatcgc
     4321 cttcggcgtc gcgttcggcc tgaacgtgct gctggacacg ctgttcatgg actcgatgac
     4381 gtcgccgttc ctggccgtgg ggatcctctt ctactcgctg ggccgctaca ccgacggctg
     4441 gcgcacctgg gccggcgtgc tggtgatcta cctgcccacc tcgaccggcc tggcgctgac
     4501 caacaccgag cagccgttcg tcctggcgat gctgtggagc gtggccctgc tggcgccgcc
     4561 gctggtggcc ggccggatca tgcgcaagcg gctggagatg caggccgagc tgcgcgacct
     4621 gaccgcccgg ctggagtccg accggggccg gcgcgagcag cgcgccatcg acagcgagcg
     4681 cgggcggatc gccgaggagc tgcagacgct ggtggccaac aacgtcagcg cgatggtggt
     4741 ccaggccggc gtcgtccggc ccgccctggc catccccgcc cccaccaccg ccgacacggc
     4801 gctggccacc atcgaggaga gcggccgcga tgcgctggtc gagatgcgcc ggctgctcgg
     4861 tgtgctgcgc cgagacgacg cggtggccga gctggccccc cagccgtcgc tggaccaggc
     4921 gttgtcgctg gtcgagcgca cccgggccga gggcctgccc gtgcacctgc acgtggacgg
     4981 cgagcccagc ccgctggccc gcggcgtcga cctggccgcc taccgcgtgc tgcaggagac
     5041 gctggactcg gcgcggcagg cggagggcag caggaaggcc caggtgctgc tggcctacgg
     5101 ggaccgcgag gtcacggtgg cggtcgcggt gcgcgacgac ggccgcggcc agcggctgct
     5161 gggcgaggac agcctgagcg ccctgcgcga gcgcgtcggc ctgtacgggg gcacgctgcg
     5221 cgcgggccgg ctcaaggacg accacggctt ccgcgtgagc gcgcggctgc cgctggaggc
     5281 cgacgcatga tcaggcggat ccggcgcctg accgagcgcg accggcgcgt gatcgacatc
     5341 gcgatcgccg tcctgatgac cgcggcggcg gtcgccgtgg tgctgacctc gccggacatc
     5401 gaggggccgc tggggctgaa cgcggccctg tacgccgtgg tcacgctggc gctggtggcc
     5461 cggcgggcgc acgtgctcga ggccacggtg gtcagcgtga gcgcgtggtt cgtgatcctg
     5521 gcgttcctga gcgcgccgtt ctacgtcccg ttcgccacct tctcgctgat gctgctgagc
     5581 tactcggccg gctcgtacgc caccggccgg cgctcgcacg ccgcgctggc gctggtcgcg
     5641 ctgaccatcc tggtcacctg catcgtcgag accccggagg acatcgcgtt cccggtggcc
     5701 atcttcggca tcgcgcccta cgtgggcggg cggatcctgc gcaaccacac cctgctgacg
     5761 cgcgagctgg cggagaaggc cgagctggcg gcccacctgg gagacgccgc cgagcgcgac
     5821 gcggtggccg ccgagcgggt gcgcgtggcc cgcgagctgc acgacgtcct ggcgcacgag
     5881 ctgagcgtca tggtcatcca ggcccagggc gcgcgccgga tgatcccgcg cgaccccgag
     5941 gcggcggccg aggtcgcgga cctgatcggg cgcacgggcc gcgacgcgct gaccgagctg
     6001 cggcgcctgt tcgggtcggt ccgccacggc gagggcgagg acctgtcggg cccgcccggc
     6061 ctgggccgcc tggacgagct ggtgggccgc gcccgcgccg ccgggctgcc cgtggagctg
     6121 cgcaccgagg gcg
//
LOCUS       DASUPW010000523         7634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1644043, whole genome shotgun sequence.
ACCESSION   DASUPW010000523 DASUPW010000000
VERSION     DASUPW010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7634
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1644043"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VFY44_11765"
     CDS             complement(<1..797)
                     /locus_tag="VFY44_11765"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5911161.1"
                     /translation="MHILYLHQFFTTREGTGGTRSYEFARHLAAAGHDVTMVTAARGE
                     SPRRYDVDGFAVVEVKGGSPDYVRATAQSFARRIAGFGGFAAAATAAAVRLPRPDVVF
                     ATSPALTVAVPGLLASRRHRVPLVFEVRDLGPRAPIEMGALRNPAARALARALEKAAY
                     RGAAHVVALSPGMREGVLEAGVDPGRVTLIPNAADLDLFGPGVAPGSLADRFGDDFVV
                     SYFGTMGEANDLDQVVRAATLLADRGERGVTFALMGDGKRRPALEAEV"
     gene            815..1417
                     /locus_tag="VFY44_11770"
     CDS             815..1417
                     /locus_tag="VFY44_11770"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX5911162.1"
                     /translation="MTRLLDITVAAAALILCSPLLLAAAVAIAVESRGGVIYRHQRIG
                     RDGVPFELWKLRTMVSGAESMGAGLYIEPGDARITRTGAFLRRFSLDELPNLVNVLRG
                     EMSVVGPRPTVAVQVDRYTPHQRRRLEVKPGITGWAQVNGRASISWPERIELDVWYVD
                     NRSVGLDLRILARTVKLLVSGQGLYSSELEQGWGEERPEE"
     gene            complement(1418..1870)
                     /locus_tag="VFY44_11775"
     CDS             complement(1418..1870)
                     /locus_tag="VFY44_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911163.1"
                     /translation="MGAIAAVSACSVAAAATPGSGTYKGLTSQKDAKSGKYQPVEVKV
                     GSKGKRVKRLRIGFQTACADGTSYVSGFFVEGRPVGKGGKFTFFSTYKPPDLNPGETA
                     DVFVEGKGRFTSAKKVKGSLDVQVNIFTAGKVGALKCRSGKVSFSARR"
     gene            complement(1941..2669)
                     /locus_tag="VFY44_11780"
     CDS             complement(1941..2669)
                     /locus_tag="VFY44_11780"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEX5911164.1"
                     /translation="MASRKEQKEQLRREREEREAEAKAAQRRKQMIGYGAGGLAVVVI
                     VVVAVVLLAGGGGGDSGGGKSGGDSAVLPEGGEVPERKIEELTPAAKAAGCTLTNYKG
                     KSREHTDDLSEKIKYDSNPPTEGKHFATPAEDGAYDEPPDIKELVHSMEHGRVIIWFK
                     KDLPEDQRADLKALFDEDSYQMLLTPDPSGMKYQVAATAWSREPTPNGTGHLLACPKY
                     TDETLDAIQAFRDEYRGNGPEAIP"
     gene            complement(2694..3527)
                     /locus_tag="VFY44_11785"
     CDS             complement(2694..3527)
                     /locus_tag="VFY44_11785"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5911165.1"
                     /translation="MTPTRHTFEFDGHTLAYDEYGAGERAVVLMPGLLFSRTMHGPLA
                     TTLAERGYRVLCLDLLGHGESDRPPEMWNYSMTLFGAQTLALLDHLEIDKAVLGGTSL
                     GANTTLEACAADQSRVQGMLIEMPVLDNALLGCAIGFTPLLLSLTFGAPAVRAWGRLA
                     SLVPRRGPLLGRMLLDWVSQDPKPSASVVQGLFFGRVAPPTAERRTLEPPTLIIGHPR
                     DPIHPFSDADMLFRELPDARMLEASSILELRLTPERLTGQIVEFIDGCWAAPASRRRA
                     A"
     gene            3645..4136
                     /locus_tag="VFY44_11790"
     CDS             3645..4136
                     /locus_tag="VFY44_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5911166.1"
                     /translation="MSQTVPLSVTVNGEKRESDVEPRQLLVYYLRDQLGLTGSHVGCD
                     TSNCGACTCHVNGESVKSCTVLAVQVEGAEVTTIEGLGQEGNLHALQEGFREKHGLQC
                     GYCTPGMIMAAADLLERNPDPSDEEIRHGLEGNLCRCTGYENIVRAVRHAAEAGKGAG
                     VSA"
     gene            4133..6547
                     /locus_tag="VFY44_11795"
     CDS             4133..6547
                     /locus_tag="VFY44_11795"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5911167.1"
                     /translation="MSVTDAPPNGGGKNFVGRSMKRREDSRLMTGRGDYIDDMVLPGM
                     LHMSVVRSPEAHANITSIDAEEARAMPGVVAVFTKEDLADLIKAPLPMAWDPPGVEIK
                     APGNWPLSGGQVKHVGDAIAVVLAETKGQAMDAADLVIADLDPLPAVVDPEKALEDGS
                     PLVWEEFGSNATHHWAVAGGDFAAAEAEAEVVVKQRVPNHRISGAPIEPRGCVGESRG
                     ESVALYSSTQIPHIARFLLAGILEMPEDKIRVVAPDVGGGFGAKLQLYGEEVLVLALA
                     KKLNRPVKYVETRSEHMSNSHHGRDQIATVTLTAKKDGTVTGCKAEVIADTGAYHQLL
                     TPFIATLGFPVAGGCYKFPSIDFSVTGVFTNKMPIDAIRGAGRPECTYWIEQTMDKLA
                     RELDMDPLELRRKNFIPKEEFPYETPLGIVYDSGDYHGALDKLLENFDLDAFRKEQAE
                     LREQGIHRGVGFSTWVEVCGLAPSRAVGPQGVGLQAAFWESAMVRVHPSGSATVYSGS
                     SPHGQGLDTSFAQIAADRLGIDPEMIDVIHGDTDSGPFGWGTYGSRSLSVGGEAIARA
                     AEKVQDKAKKVCAALLEAAPEDIELADGAFRVKGSPDKSMTMAEISGAAHIPPNELPA
                     DIEPGLEETSFYDPENFVFPFGAHACIVDVDVETGKVKVVDYYAVDDCGPAINPMIIE
                     GQIHGGIAHAIGQALYEQVVYDEEGQLVTGTFVDYALPTAAELPSFKTDRTETPSPVN
                     SLGVKGVGEAGTIAATPAVTAAVMDAVKPVKELDWLDMPLTPMRIWEALHTSEGPPST
                     EQGGAR"
     gene            6544..7368
                     /locus_tag="VFY44_11800"
     CDS             6544..7368
                     /locus_tag="VFY44_11800"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX5911168.1"
                     /translation="MITAPFDYRRASSVDEAVSLLGELGEDAKLIAGGHSLLPLMKLR
                     LAAPSTLIDIGGIGGLDTIEANGGGFRIGALTRHVTLQNSPELGVIQPVAEGIADQQV
                     RNRGTIGGSIAHGDPASDLPTAMLALEATFNVTGPSGERQIAAGEFFQDYLTTALEPQ
                     EFLTSIDVPSVDFGWGYEKFTRRAEDWAMVGVVALVKGDDVRVAFTNMGSTALRATAV
                     EEALSGGSVDADSIAAASEKAADGTEPPGDLNATPEYKAHLARVLTRRALTTAAGG"
     gene            7365..>7634
                     /locus_tag="VFY44_11805"
     CDS             7365..>7634
                     /locus_tag="VFY44_11805"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5911169.1"
                     /translation="MTFDSPEDVARALAAQGYLADPALATSVHLAGALEQPLLLEGEA
                     GVGKTEVARALAGAQGAELFRLQCHEGIDLHHALYDWDYPRQLLAI"
BASE COUNT         1132 a         2716 c         2731 g         1055 t
ORIGIN      
        1 acctccgcct ccagcgcggg ccggcgcttg ccgtcgccca tcagcgcgaa cgtcacgccc
       61 cgctcgccgc ggtcggccag gagcgtggcc gcgcgcacga cctggtccag gtcgttggcc
      121 tcgcccatgg tgccgaagta cgacaccacg aagtcgtcgc cgaagcggtc ggcaagggag
      181 cccggggcca cgccgggccc gaacaggtcc aggtccgcgg cgttggggat cagggtcacc
      241 cggcccgggt ccacgcccgc ctccagcacg ccctcgcgca tccccgggga cagcgccacc
      301 acgtgcgcgg cgccgcggta ggccgccttc tccagcgcgc gcgccagcgc ccgcgccgcc
      361 gggttgcgca gggcgcccat ctcgatcggc gcccgcggcc ccaggtcgcg cacctcgaac
      421 accagcggca cgcggtggcg gcgcgaggcc agcaggccgg gcacggccac cgtcagggcg
      481 ggagaggtgg cgaacaccac gtcggggcgg ggcaggcgca ccgcggccgc ggtggccgcc
      541 gccgcgaagc ccccgaagcc ggcgatccgg cgcgcgaagg actgggcggt ggcgcgcacg
      601 tagtcgggcg agccgccctt gacctcgacc acggcgaagc cgtcaacgtc gtagcggcgg
      661 gggctctcgc cccgggccgc ggttaccatc gtcacgtcgt gccccgcagc cgccagatgg
      721 cgcgcgaact cgtacgagcg ggtgccgccc gtgccctcgc gcgtggtgaa gaactggtgc
      781 aggtacagga tgtgcacgaa accgagaccg taacgtgacc cgcctcctgg acatcaccgt
      841 ggccgccgcc gcactgatcc tgtgctcacc gctgttgctg gccgccgcgg tggccatcgc
      901 ggtcgagtcc cggggcgggg tgatctaccg ccaccagcgg atcggccgcg acggggtccc
      961 gttcgagctg tggaagctgc gcacgatggt ctccggcgcc gagtccatgg gcgccggcct
     1021 gtacatcgag ccgggcgacg cccgcatcac gcgtaccggc gccttcctgc gccggttctc
     1081 gctggacgag ctgcccaacc tggtcaacgt gctgcgcggc gagatgtcgg tggtcggccc
     1141 acgccccacc gtggccgtgc aggtggaccg gtatacgccg caccagcgcc gccggctgga
     1201 ggtcaagccc gggatcaccg gctgggcgca ggtcaacggg cgcgcctcga tctcctggcc
     1261 cgagcggatc gagctggacg tctggtacgt cgacaaccgc agcgtcggcc tggacctgcg
     1321 catcctggcc cggaccgtga agctgctggt cagcgggcag gggctctaca gctcggagct
     1381 ggagcagggc tggggcgagg agcgaccgga ggagtgatca gcgcctggcg ctgaaggaga
     1441 ccttgccgct cctgcacttc agcgcgccga ccttgccggc ggtgaagatg ttgacctgca
     1501 cgtccaggct gcccttcacc ttcttggcgc tggtgaagcg gcccttgccc tcgacgaaca
     1561 cgtcggcggt ctcgcccggg ttcaggtcgg gcggcttgta ggtgctgaag aacgtgaact
     1621 tcccgccctt gcccacgggg cggccctcca cgaagaaccc cgagacgtag ctcgtcccgt
     1681 cggcgcaggc ggtctggaag ccgatccgca ggcgcttgac ccgcttgccc ttcgagccca
     1741 ccttgacctc gaccggctgg tacttcccgg acttggcgtc cttctgggag gtcaggccct
     1801 tgtaggtccc ggaccccggc gtggcggcgg ccgcgaccga gcaggcggac acggcggcga
     1861 tcgctcccat gcacatggcc gtccggtacc cccgcacgcg gccaacatag gcgcgaacgg
     1921 ctacctgggc aaatcgccgg ctaggggatg gcttccgggc cgttgccgcg gtactcgtcg
     1981 cggaaggcct ggatggcgtc gagcgtctcg tcggtgtact tcgggcaggc cagcaggtgg
     2041 ccggtcccgt tgggcgtggg ctcacgcgac caggcggtgg ccgccacctg gtacttcatc
     2101 cccgaggggt ccggggtcag cagcatctgg tagctgtcct cgtcgaacag cgccttcagg
     2161 tccgcgcgct ggtcctcggg caggtccttc ttgaaccaga tgatcacgcg cccgtgctcc
     2221 atgctgtgca cgagctcctt gatgtcgggc ggctcgtcgt aggcgccgtc ctcggccggg
     2281 gtggcgaagt gcttgccctc ggtcggcggg ttgctgtcgt acttgatctt ctcgctcagg
     2341 tcgtcggtgt gctcgcggct cttgcccttg tagttggtca gcgtgcagcc cgccgccttc
     2401 gcggccggcg tgagctcctc gatcttgcgc tcgggcacct cgccaccctc gggcaggacc
     2461 gcgctgtcgc cgccgctctt gccgccgccc gagtcgcccc cgccgccgcc ggccaggagc
     2521 accacggcca cgaccacgat gaccaccacg gccagcccgc cggcgccgta gccgatcatc
     2581 tgcttgcgcc gctgcgcggc cttggcctcg gcctcgcgct cctcgcgctc gcgcctcagc
     2641 tgctccttct gctccttgcg gctggccatc agcggtcgaa gagtagtgag ccgctaggcg
     2701 gcgcgtcgcc gtgacgccgg cgcggcccag cagccgtcga tgaactccac gatctggccg
     2761 gtcagccgct ctggcgtcag gcgcagctcc aggatggacg aggcctcgag catgcgcgcg
     2821 tcgggcagct cgcggaagag catgtcggcg tccgagaacg ggtggatcgg atcgcgcggg
     2881 tggccgatga tcagcgtggg cggctcgagg gtgcgccgct ccgcggtcgg cggggccacg
     2941 cggccgaaga acaggccctg gaccacggac gccgacggct tggggtcctg gctgacccag
     3001 tccagcagca tccggcccag cagagggccg cgccgcggga ccagcgaggc caggcggccc
     3061 cacgcgcgca cggcgggcgc gccgaaggtc agcgacagca gcagcggggt gaagccgatc
     3121 gcgcaaccca gcagcgcgtt gtccagcacc ggcatctcga tcagcatccc ctggacgcgc
     3181 gactggtcgg ccgcgcacgc ctcgagcgtg gtgttggcgc ccagcgaggt gccgcccagc
     3241 acggccttgt cgatctcgag gtggtccagc agggccagcg tctgggcacc gaacagcgtc
     3301 atcgagtagt tccacatctc gggcgggcgg tccgactcgc cgtggcccag caggtccagg
     3361 cacagcaccc ggtagccgcg ctcggccagc gtggtggcca gcgggccgtg catggtgcgc
     3421 gagaacagca gccccggcat cagcaccacg gcccgttctc cggccccgta ttcgtcgtag
     3481 gccagggtgt ggccgtcgaa ctcgaaggtg tgtcgggtgg gggtcatcgg cgacgccaca
     3541 ttactcccgc gcgcgaaccg agcgccacgt tgcgccaagg tttccaccgg gcttgcgcga
     3601 ggaactacgg taggagcacc acgaactcct ggggaggaga cgagatgagt cagaccgtgc
     3661 cgttgtccgt caccgtgaac ggtgagaaac gcgagtcgga tgtcgagccc cgtcagctgc
     3721 tcgtctacta cctgcgtgac cagctcggcc tgaccggcag ccacgtgggc tgcgacacct
     3781 ccaactgcgg cgcctgcacc tgccacgtga acggcgagtc ggtcaagagc tgcacggtgc
     3841 tggcggtcca ggtcgagggt gccgaggtca ccaccatcga ggggctcggc caggagggca
     3901 acctgcacgc gctgcaggag ggcttccgcg agaagcacgg cctccagtgc ggctactgca
     3961 cgcccggcat gatcatggcc gcggccgacc tgctggagcg caaccccgat ccctccgacg
     4021 aggagatccg ccacggcctg gagggcaacc tctgccgctg caccggctac gagaacatcg
     4081 tgcgcgccgt gcgtcacgcg gcggaagccg gcaagggcgc gggggtgtcg gcatgagcgt
     4141 caccgacgca ccgcccaacg gcggcggcaa gaacttcgtc ggccggtcga tgaagcggcg
     4201 cgaggactcg cgcctgatga ccggccgcgg cgactacatc gacgacatgg tcctccccgg
     4261 gatgctgcac atgtcggtcg tccggtcgcc ggaggcgcac gccaacatca cctcgatcga
     4321 cgccgaggag gccagggcga tgccgggcgt cgtggcggtc ttcaccaagg aggacctggc
     4381 cgacctgatc aaggcgccgc tgcccatggc ctgggacccc ccgggcgtcg agatcaaggc
     4441 gcccgggaac tggccgctgt ccggcggcca ggtcaagcac gtgggtgacg cgatcgcggt
     4501 cgtgctggcc gagaccaagg gccaggccat ggacgccgcc gacctggtga tcgccgacct
     4561 ggacccgctg cccgcggtgg tcgatcccga gaaggcgctc gaggacggtt ccccgctggt
     4621 gtgggaggag ttcggctcca acgcgaccca ccactgggcc gtggccggcg gcgacttcgc
     4681 ggctgccgag gccgaggccg aggtcgtggt caagcagcgc gtgcccaacc accgcatctc
     4741 gggcgcgccg atcgagccgc gcggctgcgt cggcgagtcc cgcggcgaga gcgtggcgct
     4801 gtactcgtcg acccagatcc cgcacatcgc acggttcctg ctggcaggca tcctcgagat
     4861 gcccgaggac aagatccgcg tggtcgcgcc cgacgtgggc ggcggcttcg gggccaagct
     4921 ccagctctac ggcgaggagg tgctggtcct ggcgctggcc aagaagctca accggcccgt
     4981 caagtacgtc gagacgcgct cggagcacat gtccaacagc caccacgggc gcgaccagat
     5041 cgcgacggtc acgctcacgg cgaagaagga cggcacggtc acgggctgca aggcggaggt
     5101 catcgccgac accggcgcct accaccagct gctcacgccg ttcatcgcga cgctgggctt
     5161 cccggtggcg ggtggctgtt acaagttccc gtcgatcgac ttctcggtga ccggcgtgtt
     5221 caccaacaag atgccgatcg acgccatccg tggcgcgggc cggcccgagt gcacgtactg
     5281 gatcgagcag accatggaca agctcgcgcg cgagctggac atggacccgc tggagctccg
     5341 gcgcaagaac ttcatcccca aggaggagtt cccgtacgag accccgctgg gcatcgtcta
     5401 cgactccggc gactaccacg gggccctgga caagctgctg gagaacttcg acctggacgc
     5461 gttccgcaag gagcaggccg agctgcgcga gcagggcatc caccgcggcg tcggcttctc
     5521 gacctgggtc gaggtgtgcg gcctggcgcc gtcacgcgcc gtcggcccgc agggcgtcgg
     5581 cctgcaggcc gcgttctggg agtcggccat ggtgcgcgtg cacccgtcgg gctcggccac
     5641 cgtctactcg ggctcgtcgc cgcacggcca gggcctggac acctcgttcg cgcagatcgc
     5701 ggccgaccgg ctgggcatcg acccggagat gatcgacgtg atccacggcg acaccgactc
     5761 cggcccgttc ggctggggca cgtacgggtc gcgctccctg tcggtgggcg gcgaggccat
     5821 cgcccgcgcg gccgagaagg tccaggacaa ggccaagaag gtgtgcgcgg cgctgctgga
     5881 ggccgcgccc gaggacatcg agctggccga cggggcgttc cgcgtgaagg gctcgccgga
     5941 caagtcgatg accatggccg agatctccgg ggcggcgcac atcccgccca acgagctgcc
     6001 ggccgacatc gagccgggcc tcgaggagac gtcgttctac gacccggaga acttcgtgtt
     6061 cccgttcggc gcgcacgcgt gcatcgtcga cgttgacgtg gagacgggga aggtcaaggt
     6121 ggtcgactac tacgcggtcg acgactgcgg cccggccatc aacccgatga tcatcgaggg
     6181 ccagatccac ggcggcatcg cgcacgccat cggccaggcc ctgtacgagc aggtggtcta
     6241 cgacgaggag ggccagctgg tcaccggcac gttcgtggac tacgcgctgc ccaccgcggc
     6301 cgagctgccg tcgttcaaga ccgaccgcac cgagacgccg tcgcccgtga actcgctggg
     6361 cgtcaagggc gtcggcgagg cgggcaccat cgcggccacg ccggcggtga ccgccgcggt
     6421 catggacgcg gtcaagccgg tcaaggagct ggactggctg gacatgccgc tcacgccgat
     6481 gcggatatgg gaggcgctgc acaccagcga aggaccaccc tcgaccgagc agggaggtgc
     6541 ccgatgatca ccgcaccgtt cgactaccgc cgcgcgtcgt cggtcgacga ggccgtctcg
     6601 ctcctcggcg agctgggcga ggacgccaag ctcatcgccg gcggccactc gctgctgccg
     6661 ctgatgaagc tccgcctggc ggcgcccagc acgctgatcg acatcggcgg catcggcggg
     6721 ctggacacca tcgaggccaa cgggggcggg ttccggatcg gcgcgctcac gcggcacgtg
     6781 acgctgcaga actcgcccga gctgggcgtg atccagccgg tcgcggaggg catcgccgac
     6841 cagcaggtgc gcaaccgcgg caccatcggc ggctcgatcg cccacggcga cccggcgtcg
     6901 gacctgccca cggccatgct cgccctggag gccacgttca acgtgaccgg cccgagcggt
     6961 gagcgccaga tcgcggccgg ggagttcttc caggactacc tgaccaccgc gctcgagccg
     7021 caggagttcc tgacctcgat cgacgtcccg tcggtcgact tcggctgggg ctacgagaag
     7081 ttcacccgcc gtgccgagga ctgggccatg gtcggggtgg tggcgctcgt gaagggcgac
     7141 gacgtgcgcg tggccttcac caacatgggc tcgacggccc tgcgggcgac cgccgtcgag
     7201 gaggcgctca gcggcggctc cgtcgacgcc gactcgatcg ccgccgcgtc cgagaaggcc
     7261 gccgacggca ccgagccgcc cggcgacctg aacgccacgc cggagtacaa ggcgcacctg
     7321 gcgcgcgtgc tgacccggcg cgcgctgacg accgccgccg ggggatgacg ttcgactccc
     7381 ctgaagacgt agcccgggcc ctcgcggccc agggctacct cgccgacccc gcgctggcca
     7441 cgtccgtgca cctggccggc gcgctggagc agccgctgtt gctggagggc gaggcgggag
     7501 tgggcaagac cgaggtggca cgggcgctgg ccggagccca gggcgccgag ttgttccggc
     7561 tccagtgcca cgagggcatc gacctgcacc acgcgctcta cgactgggac tacccgcgcc
     7621 agctgctggc catc
//
LOCUS       DASUPW010000524         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_536422, whole genome shotgun sequence.
ACCESSION   DASUPW010000524 DASUPW010000000
VERSION     DASUPW010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536422"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VFY44_11810"
     CDS             <1..524
                     /locus_tag="VFY44_11810"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5911170.1"
                     /translation="AVLFYRSVLGLEPRSAQDVAAPHGLLSSRALEAGDGRLRLAITV
                     PRLGGGDGRLGELQHVAFAGDDVLDTAHRMRRRGVPLLAVPDNYYDDLEARTGLEPER
                     IGALRAAGVLYDADGKGGEFLQLFTEMIGDRLFFEVVQRTGGYQGYGAANSPVRLAAQ
                     LNNATQEGRRVHA"
     gene            521..1957
                     /locus_tag="VFY44_11815"
     CDS             521..1957
                     /locus_tag="VFY44_11815"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX5911171.1"
                     /translation="MSTQGTITREPPAAPLEESMVRDLPEPEGLRRLIGPSVILVGVG
                     VASGEYILFPFIASKAGLAFLWAAIAGVAMQFFINMEIERYTLATGQTALTGFTRLWK
                     PWSLVLVFLALLATLWPGWATSAATITTFAVGGGDVNTIAIAGLLAIGVALTSSPVVY
                     QTVERLELLKVGALLVFVVIALFAAVSGTAYRDTTEVATSFGTFPGEIELAILVGALA
                     AAGAGGANNLVQSNWIRDKGFGMGRYAPRIVSPITGEEEAALSGQRYEFPEDEANLSR
                     WREWWKKANLEQFVSFALVAAVSITAFSLIAYSTVYGNPDLPESSDFSFISLEADVLD
                     DKVGSWFGTLFLAIGAISLFAAALGIVDYVSRLVADAVRCGYTEGSERWTESRLYFIT
                     VWTMVIVGSSILLAGFDQPLVLVTISTVLGGFIMFVYSSLLVVTNRRYLPKSLQLGGY
                     RLVIMGISIVVLGATSVIVGIDQFGNLL"
BASE COUNT          271 a          722 c          730 g          339 t
ORIGIN      
        1 aggcggtgct gttctaccgc tcggtgctgg gcctggagcc gcgctcggcc caggacgtgg
       61 ccgcgccgca cggcctgctc agctcgcgcg cgctggaggc cggcgacggc cgcctgcgcc
      121 tggccatcac cgtcccgcgg ctgggcggcg gcgacggccg gctgggcgag ctgcagcacg
      181 tggccttcgc cggcgacgac gtgctggaca ccgcgcaccg catgcgccgc cgtggcgtgc
      241 cgctgctggc ggtgcccgac aactactacg acgacctcga ggcgcgcacg ggcctggagc
      301 ccgagcgcat cggcgcgctg cgcgccgccg gggtgctcta cgacgccgac gggaagggcg
      361 gcgagttcct gcagctgttc acggagatga tcggcgaccg cctgttcttc gaggtcgtcc
      421 agcgcaccgg cgggtaccag ggctacgggg cggccaactc ccccgtgcgc ctggcggccc
      481 agctgaacaa cgccacacag gaggggagac gagtacacgc atgagcaccc aaggaaccat
      541 cacgcgggaa ccacccgccg cacccctgga ggaatccatg gtgcgcgacc tgcccgagcc
      601 cgagggcctg cggcggctga tcggccccag cgtgatcctg gtgggcgtag gggtggcctc
      661 cggcgagtac atcctgttcc cgttcatcgc gtcgaaggcc gggctcgcgt tcctgtgggc
      721 cgccatcgcc ggcgtggcca tgcagttctt catcaacatg gagatcgagc gctacacgct
      781 ggccaccggc cagaccgcgc tcaccggctt cacgcggctg tggaagccgt ggtcgctggt
      841 cctggtgttc ctggcgctgc tggccaccct ctggccgggc tgggccacgt ccgcggccac
      901 catcaccacc ttcgccgtcg gcggcggcga cgtgaacacc atcgccatcg ccgggctgct
      961 ggccatcggg gtggcgctga ccagctcgcc cgtcgtctac cagacggtcg agcgcctgga
     1021 gctgctgaag gtcggcgcgc tgctggtgtt cgtggtgatc gccctgttcg ccgcggtcag
     1081 cggcaccgcc taccgcgaca ccaccgaggt cgcgacgtcg ttcggcacct tccccggtga
     1141 gatcgagctg gcgatcctgg tcggcgcgct ggccgcggcc ggcgccggcg gcgcgaacaa
     1201 cctggtccag agcaactgga tccgcgacaa gggcttcggc atgggccgct acgcgccgcg
     1261 gatcgtctcg cccatcaccg gtgaggaaga ggcggccctg tcgggccagc ggtacgagtt
     1321 ccccgaggac gaggccaacc tgtcgcgctg gcgcgagtgg tggaagaagg cgaacctcga
     1381 gcagttcgtc tccttcgccc tggtcgcggc ggtgtcgatc accgcgttct cgctgatcgc
     1441 ctactccacg gtgtacggca accccgacct gcccgagtcc agcgacttca gcttcatctc
     1501 gctggaggcc gacgtgctgg acgacaaggt cggctcgtgg ttcgggacgc tgttcctggc
     1561 catcggcgcc atcagcctgt tcgccgccgc gctgggcatc gtcgactacg tgtcgcggct
     1621 ggtggccgac gcggtccgct gcgggtacac cgagggcagc gagcgctgga ccgagagccg
     1681 cctgtacttc atcacggtct ggacgatggt catcgtgggc agcagcatcc tgctggcggg
     1741 cttcgaccag ccgctggtcc tggtcaccat ctccacggtc ctgggcggct tcatcatgtt
     1801 cgtgtactcg agcctgctgg tggtcaccaa ccgccgctac ctgccgaagt cgctgcagct
     1861 gggcggctac cggctggtga tcatgggcat ctcgatcgtg gtcctgggcg ccacgtcggt
     1921 catcgtcggc atcgaccagt tcgggaacct gctgtgaccg cgcggttcgg agcccggctg
     1981 ctgatcgccc tgttcgtctg ggcggcgttc gtgggggtcg cgtacgcggt ggggctgggt
     2041 taggccgtca tccccgggca ac
//
LOCUS       DASUPW010000525         2687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_813433, whole genome shotgun sequence.
ACCESSION   DASUPW010000525 DASUPW010000000
VERSION     DASUPW010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813433"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            386..1627
                     /locus_tag="VFY44_11820"
     CDS             386..1627
                     /locus_tag="VFY44_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX5911172.1"
                     /translation="MEVLYPRCAGLDVHKATVVAAVRLVAGGKVAREVRTFATTTAGL
                     LALAAWLGENGCTHVAMEATGVYWKPVWHVLAEGGFDLVLANAAQVKNVPGRKTDVND
                     ATWLAELLAHGLVRASFVPDAPTQELRSLLRTRKQLVRERASHVLRLQKTLEDANVKL
                     DAVLSDLLGRSGRAMLEALVAGETDPEKLAAFAHPRLAATPERLREALRGRVTAHHRF
                     LLRLHLDQVDALEAAIGRIDREVEERLAPFSTAVDLLASIPGVGALAARVIVAEIGLD
                     MGRFPTAGHLVSWAGLCPRNDESAGKRLSARLRKGAPWLKTILVQCAWAASRKKASYL
                     QAQYHRLRARRGARKAVCAVAASILTAAYHMLKDGTAYRDLGPGHLDRRSRETQARRL
                     VRRLADLGYAVELAPLEPAHA"
     gene            1699..2217
                     /locus_tag="VFY44_11825"
     CDS             1699..2217
                     /locus_tag="VFY44_11825"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5911173.1"
                     /translation="MHPPIFVRELTGAERGQLEAGLRAASAFTVRRAQVVLLSAAGRQ
                     PRAIAQGLCCAVQTVRNAIHAFNSRGLVALTAGSPRPKSAAPVLDRTKRERLRAILHQ
                     SPRAFGQTRSTWTLAVLAQVAHEQGLSATVLSDETIRRALGRLEVGWKRAKRWLTSPD
                     PDYARKKVAATA"
     gene            2283..2672
                     /locus_tag="VFY44_11830"
     CDS             2283..2672
                     /locus_tag="VFY44_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911174.1"
                     /translation="MAQPALHAWTAGEPLRLGLHPADRADLDPKALACYGLWLPARGR
                     TLLRFVDGRPISPVTCAFLAWAAAQLAAEGVRVLALIWDNASWHISREVRDWVRRHNR
                     EVKRTGHGCRLLYRSTVLRHRSALTRP"
BASE COUNT          360 a          978 c          974 g          375 t
ORIGIN      
        1 cacccgaacg gccgcaacgg ctccagcacg cgctcctgat gtgcccgtac tcctcccgga
       61 tcctgcgctc ctggcgggcg aggtagctcg gtaagaccac tcgtagacgc acatggcgcg
      121 gtatcccacg gtgggcaggg ccaacgcatg agttgctcac cggcgtgcgc cgacccgccg
      181 gggatgaaac cgggatagag tggcgtggcg ccgccggcgg cgaggtcggc tttgtcttgg
      241 tgcacacgag cccgcgcatg agttggaccc ggggcctctc gagcaccccc gattgtgcca
      301 cgggccggag tccggccccg gagcacgtct cctagattcc tcccccgagg gccccacgcc
      361 ggcggcgcgc cgagaggagg gagccatgga ggtcctttac ccgcgctgcg ccggcctcga
      421 cgtgcacaag gcgaccgtgg tcgccgccgt gcgcctggtc gccggcggca aggtcgcccg
      481 ggaggtgcgg acgttcgcca ccacgaccgc gggcctgctc gccctggcgg catggctggg
      541 cgagaacggc tgcacccacg tcgcgatgga ggcgaccggg gtctattgga agccggtctg
      601 gcacgtcctc gccgagggcg ggttcgacct ggtgctggcg aacgcggcac aggtcaagaa
      661 cgtgcccggg cgcaagacgg acgtgaacga cgcgacctgg ctcgccgagc tgctggcgca
      721 cggtctcgtc cgcgccagct tcgtcccgga cgcgccgacg caggagctgc gctcgctcct
      781 gcgcacccgc aagcagctcg tccgcgagcg ggcgagccac gtgctgcggc tgcagaagac
      841 gctcgaggac gcgaacgtca agctcgacgc ggtgctgtcc gacctgctgg gcaggagcgg
      901 ccgggcgatg ctcgaggcct tggtggcagg cgagaccgac cccgagaaac ttgcggcgtt
      961 cgcccacccg cggctcgcgg cgacgccgga gcggctgcgg gaggccctgc gcggtcgggt
     1021 gacggcgcat caccgcttcc tgctgcggct gcacctcgac caggtcgatg cgctggaggc
     1081 ggcgattggg cggatcgacc gcgaggtcga ggagcggctc gcccctttta gcaccgcggt
     1141 cgacctgttg gcctcgatcc ccggcgtcgg cgcgctcgcg gcccgggtga tcgtggccga
     1201 gatcggcctc gacatgggcc ggttcccgac cgccggtcat ctggtctcct gggccgggct
     1261 gtgcccgcgc aacgacgaga gcgccggcaa gcgcctctcc gcccggctgc gcaagggcgc
     1321 gccctggctc aagaccatcc tcgtccagtg cgcctgggcg gcgagccgca agaaggccag
     1381 ctacctgcag gcgcagtacc accgcctgcg cgcccgccgc ggcgcgagga aggcggtgtg
     1441 cgcggtggcg gcctcgatcc tgaccgccgc ctaccacatg ctgaaggacg gcaccgccta
     1501 ccgggacctc ggccccggcc acctcgatcg gcgctccagg gagacccagg cccggcggct
     1561 cgtccgccgg ctcgccgacc tcggctacgc cgtcgagctg gcgcccctcg agccagccca
     1621 cgcgtgagcc cagtttcttc ctagtgcaat cccacggtag ctcttgtgcg agggcattgc
     1681 gggccgcatc tgatcgggat gcacccaccg atcttcgttc gtgagctgac cggcgcggag
     1741 cgcggccagc ttgaggccgg cctgcgcgcg gcgagcgcat tcaccgtgcg gcgtgcccag
     1801 gtcgtgctgc tgagcgcggc ggggcgccag ccgcgggcga tcgcgcaggg cttgtgctgc
     1861 gcggtgcaga ccgtgcgcaa cgccatccac gcgttcaaca gccgcggcct tgtggccctg
     1921 accgccggct cgccgcgccc caagagcgcc gccccggtgc tcgatcggac caagcgggag
     1981 cggctgcgcg ccatcctgca ccagtcgccg cgcgccttcg gccagaccag gagcacctgg
     2041 acgctggccg tgctggcgca ggtcgcgcac gagcaggggc tgagcgcgac cgtcctgtcg
     2101 gacgagacga tccggcgggc gttggggcgg ctggaggtgg gctggaagcg cgccaagcgc
     2161 tggctgacca gccccgatcc cgactacgct cgcaaaaaag tcgccgcgac cgcctgatcc
     2221 ggcgggcgca aagccggccg ggctgggcgc tcggctttgc cgacgaggtc tggttctccc
     2281 gcttggcgca gccggcgctg catgcctgga cggcgggcga gcccctgcgc ctcggcctgc
     2341 accccgcaga tcgcgccgat ctcgatccca aggccctggc ctgctacggc ctgtggctgc
     2401 ctgcacgcgg gcggaccctg ctgcgcttcg tcgacgggcg gccgatcagc ccggtgacct
     2461 gcgccttcct cgcctgggct gccgcgcagc tcgcggccga gggcgtccgg gtcctggcac
     2521 tgatctggga caacgcttcc tggcatatca gccgcgaggt gcgggactgg gtcaggaggc
     2581 acaatcgcga ggtcaagcgg accggccacg gttgccgcct tctgtaccgg tccacagttc
     2641 tgagacaccg ctctgcgctt acgcggccat aggcagggcc gcggtct
//
LOCUS       DASUPW010000526         3415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_536580, whole genome shotgun sequence.
ACCESSION   DASUPW010000526 DASUPW010000000
VERSION     DASUPW010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536580"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..571
                     /locus_tag="VFY44_11835"
     CDS             131..571
                     /locus_tag="VFY44_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911175.1"
                     /translation="MSEQQPSEEELRAALEEQMRNIRVEDVVLQTTATLINLAGRRLG
                     LAAEPGQDPSGDVDLQQAKLAIDGAMALGPLCPQDQAEPIKQALSQLQMAYSRLAGGP
                     GGPAPEGAQPGQEAPAPDPEAEAKAREEQERAKARSKLWTPGSD"
     gene            550..1365
                     /locus_tag="VFY44_11840"
     CDS             550..1365
                     /locus_tag="VFY44_11840"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF005876.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEX5911176.1"
                     /translation="MDAGQRLSAPVGIFGGSGFYSLLDDVEELSLDTPYGPPSAPIRM
                     GQIEGTPVAFMPRHGDHHELPAHRVNYRANLWAMKEAGVRRIVGPCACGTLKAELEPG
                     TFVVCDQFVDRTSGRADTFYDGPRTTHVAAADPYCADLRATLVRAARELGIPVVDGGT
                     VVVIQGPRFSTRAESRWFAQAGWDVVNMTQYPEAWLARELELCYANVSLVTDYDAGLE
                     GKPDVPPVSAEEAFRVFADNLDKLRALLFRAVPTVGPQPDDSCARALASAIVH"
     gene            1426..>3415
                     /locus_tag="VFY44_11845"
     CDS             1426..>3415
                     /locus_tag="VFY44_11845"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001951.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX5911177.1"
                     /translation="MTDFLTDYGIVIALICAGAAVVYGLTTTRWLLAKSPGNDEMQRI
                     SKAVQDGASAYLLRQYQIIGVVAVILAIVLAVALDVRTAIGFIVGGAFSGAAGFIGMN
                     VSVRANTRVAETARGGVGPALEVAFKGGAITGMLVAGLALLGVAGYYGILLLTGTEEK
                     EAIDALVGLGFGGSLISVFARLGGGIFTKAADVGADVVGKIEAGIPEDDPRNPAVIAD
                     NVGDNVGDCAGMAADLFETYAVTAVAVMLLGVLTFDNIGAVGVYPLVIGGVSIIASII
                     GTYAVRSASGNVERALYQGLIVSGVIAAIAFLPISLWLMDDLSFKDGASSLITGGGDV
                     ASGFDFWLCSLIGIAITAFLFVITDYYTSTRFNPVKSTARASQTGHATNIIQGLAQGF
                     QSTAAPAIVLALGILAANELAGIYGIGVAVMAQLSLTGLIVALDAFGPITDNAGGIAE
                     MAELPEEVRNVTDPLDAVGNTTKAVTKGYAIGSAVLAALVLFAAFVEELREEAIKEGV
                     TLQGFDISQPEVLVGLLVGGMMVFLFVALSIESVGRAGGLVVDEVRRQFKEKPGIMDR
                     TEEPDYRGAVAIVTAAAQKEMILPALIPIVITVVVGLLSYQALGGLLIGVIIVGFFTA
                     VSMTAGGGAWDNAKKLIEDGEFGGKGSEAHEASITGDTV"
BASE COUNT          491 a         1198 c         1211 g          515 t
ORIGIN      
        1 tcagccttcc ggcgcgcaac ggcgcccagc ggcagcgcga cgcgttctac agcggtatga
       61 cgatcagaca ggtgttcgcg gcagaggtgc tcgcggcaca gagaagctat gccggccagg
      121 gggtcgccac atgagtgagc agcagccgag cgaggaagag ctccgcgcgg cgctcgagga
      181 gcagatgcgc aacatccggg tcgaagacgt ggtcctgcag accacggcga cgctgatcaa
      241 cctggccggg cgccggctcg gcctggccgc ggagccgggc caggacccct ccggcgatgt
      301 ggacctccag caggccaagc tggccatcga cggcgcgatg gcgctgggcc ccctgtgccc
      361 gcaggaccag gccgagccga tcaagcaggc cctgtcccag ctccagatgg cctactcacg
      421 gctggccggc ggacccggcg ggcccgcgcc cgagggtgcc cagccgggcc aggaggcccc
      481 ggcgcctgac cccgaggccg aggccaaggc gcgtgaggag caggagcgcg cgaaggcgcg
      541 gtccaagctg tggacgccgg gcagcgactg agcgccccgg taggcatctt cggcggcagc
      601 gggttctact cgctgctgga cgacgtcgag gagctttcgc tcgacacccc gtacgggccg
      661 ccctcggcgc cgatccggat ggggcagatc gagggcaccc cggtggcgtt catgccccgc
      721 cacggcgacc accacgagct gcccgcgcac cgggtgaact accgggccaa cctctgggcc
      781 atgaaggagg cgggggtgcg ccggatcgtc gggccgtgcg cctgcggcac gctcaaggct
      841 gagctggagc cgggcacgtt cgtggtctgc gaccagttcg tggaccgcac cagcggccgt
      901 gccgacacct tctacgacgg cccccggacc acccacgtgg cggcggcgga cccctactgc
      961 gccgacctgc gcgcgacgct cgtgcgggcc gcccgcgagc tgggcatccc ggtggtggac
     1021 gggggcaccg tggtggtcat ccaggggccc cggttctcga cccgcgccga gtcgcgctgg
     1081 ttcgcccagg ccggctggga cgtggtcaac atgacccagt acccggaggc gtggctggcc
     1141 cgggagctcg agctctgtta cgcgaacgtc tcgctggtga ccgactacga cgccggtctg
     1201 gaggggaaac cggacgttcc cccggtatcg gcggaggagg cgttccgcgt ctttgccgac
     1261 aacctcgata agctgcgcgc gctcttgttc cgggcggtgc cgactgtggg gcctcagccg
     1321 gacgattcgt gcgccagggc tctggccagc gcgatcgtcc actaaggcgc gcctgccgcg
     1381 gctcccggcc cgcctgacag cgatccatac cggaggcatc ttcccttgac cgactttctg
     1441 accgactacg gcatcgtcat cgcgctgatc tgcgccggtg ctgccgtggt gtacggactc
     1501 acgaccacgc gctggctgct ggccaagtcg cccggcaacg acgagatgca gcgcatctcg
     1561 aaggcggtgc aggacggcgc gagcgcctac ctgctgcgcc agtaccagat catcggcgtg
     1621 gtggccgtga tcctggccat cgtcctggcg gtcgccctcg acgtgcgcac cgccatcggc
     1681 ttcatcgtcg gcggtgcctt ctcgggcgcc gcgggcttca tcggcatgaa cgtgtcggtg
     1741 cgggccaaca cccgggtggc cgagaccgcc cgtggcggcg tcggccccgc gctggaggtc
     1801 gccttcaagg gcggcgccat cacgggcatg ctcgtcgccg gcctggccct gctgggcgtg
     1861 gccgggtact acggcatcct gctgctcacc ggcaccgagg agaaggaggc catcgacgcg
     1921 ctcgtgggcc tcggattcgg tggctcgctg atctccgtct tcgcccgtct gggcggcggc
     1981 atcttcacga aggcggccga cgtgggcgcc gacgtggtcg gcaagatcga ggccggcatc
     2041 cccgaggacg acccgcgcaa cccggcggtc atcgccgaca acgtgggcga caacgtcggc
     2101 gactgcgccg gcatggcggc cgacctgttc gagacctacg cggtgacggc ggtggccgtg
     2161 atgctgctgg gcgtcctgac cttcgacaac atcggcgccg tgggcgtcta cccgctggtc
     2221 atcggcggcg tgtccatcat cgcctcgatc atcggcacct acgccgtccg gagcgccagc
     2281 ggcaacgtcg agcgcgcgct gtaccagggc ctgatcgtgt ccggcgtgat cgccgcgatc
     2341 gcgttcctgc ccatctcgct gtggctgatg gacgacctgt cgttcaagga cggcgcctcc
     2401 agcctgatca ccggtggcgg cgacgtggcc agcggcttcg acttctggct ctgctcgctg
     2461 atcggcatcg ccatcacggc gttcctgttc gtgatcaccg actactacac ctcgacccgc
     2521 ttcaacccgg tcaagagcac cgcgcgtgcc tcgcagaccg gtcacgcgac caacatcatc
     2581 cagggcctgg cccagggctt ccagtccacg gccgccccgg cgatcgtgct ggcgctgggc
     2641 atcctggccg ccaacgagct ggccggcatc tacggcatcg gcgtcgccgt catggcgcag
     2701 ctgtcgctga cgggcctgat cgtggccctg gacgcattcg ggccgatcac cgacaacgcc
     2761 ggcggcatcg ccgagatggc ggagctgccc gaggaagtcc gcaacgtgac cgacccgctg
     2821 gacgcggtcg gcaacaccac caaggccgtg accaagggct acgccatcgg ctccgccgtg
     2881 ctggccgccc tggtgctgtt cgcggcgttc gtcgaggagc tgcgtgagga ggccatcaag
     2941 gagggcgtga ccctccaggg cttcgacatc tcgcagcccg aggtgctcgt cggcctgctg
     3001 gtcggcggca tgatggtgtt cctgttcgtc gcgctctcga tcgagtcggt cgggcgcgcg
     3061 ggtggcctgg tggtggacga ggtccggcgc cagttcaagg agaagccggg catcatggac
     3121 cgcaccgagg agcccgacta ccgcggcgcc gtggccatcg tgaccgcggc cgcgcagaag
     3181 gagatgatcc ttcccgcgct gatcccgatc gtgatcaccg tggtcgtcgg cctgctgtcc
     3241 taccaggcgc tcggcggcct gctgatcggc gtcatcatcg tcggcttctt caccgccgtc
     3301 tccatgaccg ccggtggcgg cgcgtgggac aacgccaaga agctgatcga ggacggcgag
     3361 ttcggcggca agggctccga ggcccacgag gcctcgatca cgggcgacac ggtgg
//
LOCUS       DASUPW010000527         2469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_674347, whole genome shotgun sequence.
ACCESSION   DASUPW010000527 DASUPW010000000
VERSION     DASUPW010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674347"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VFY44_11850"
     CDS             complement(<1..302)
                     /locus_tag="VFY44_11850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5911178.1"
                     /translation="MTGDAVLSAEGLTKRYGDRVALREVSLSAARGELVAVIGPNGAG
                     KTTLLSILAGIQQPDGGSVSLGPRQIGWVPQQAALYGKLTVAENLRLFARLEKADDP"
     gene            330..1118
                     /locus_tag="VFY44_11855"
     CDS             330..1118
                     /locus_tag="VFY44_11855"
                     /inference="COORDINATES: protein motif:HMM:NF024674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEX5911179.1"
                     /translation="MKVLFVGDVVGGIGRRTLAALLPGLRERHAPDFVVVNGENAAGG
                     VGITQKTARQVLDMGVDVITLGNHAYRHTEVYEFLDTEERIVRPANYPRASPGRGVTV
                     VERDGVALGVVNLSGSLFVEAARSAFAEVDAALSDLRGRATHVLVDMHAEATSEKVAM
                     GWHVDGRVTACVGTHTHIPTADCRVLPGGTAYCTDVGMTGPRGGVIGVKRESALERFR
                     TMTNVRFETSDQDPWINAVLIEAGADGKATAIHQILEPAPPGTT"
     gene            complement(1030..2253)
                     /locus_tag="VFY44_11860"
     CDS             complement(1030..2253)
                     /locus_tag="VFY44_11860"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX5911180.1"
                     /translation="MRAAAGAAALLAAGFALTFLVGPWDDESISDLPLYSSYAQIFLD
                     GHLPYRDVAFEYPPLAWPVLTVAGLAGDQDAYRIAFAVLAVALAVAMVALCGRLATAT
                     GGDRTAALLGAAAAPVLCGAMLRTHFDLVPVVLTLAALALLCAARPRAGMAVLGVAVV
                     VKLDPLVVAPVALAWLVARDERRAAVEGAAALALVVVVAYGAVATLSPAGVADSFTYH
                     LDRPVQVESTPAIVVRALDSLGLGAATPNDGFRSDGLDHPAGDLVAALFTALLAAAVA
                     VLATGVARAPDERALVLASLAATVAFACLGKVLSPQFLIWVMPLLALALAWRMRALAA
                     LSALAVVLTLFEFPGLYREVVDRDAGALALVVVRDLALLGVVGVAMWSLGERARGSGG
                     SPWPSRPRRPRSAPR"
     gene            complement(2250..>2469)
                     /locus_tag="VFY44_11865"
     CDS             complement(2250..>2469)
                     /locus_tag="VFY44_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911181.1"
                     /translation="LKAGAGLLAVAVFFVPRRRDAFQVAALGAAVVIAVQLTVSHWFY
                     LYIVWFAPLALVALMAPWRTGPEPSAPG"
BASE COUNT          388 a          935 c          900 g          246 t
ORIGIN      
        1 gggtcgtcgg ccttctcgag cctggcgaac aggcgcaggt tctcggccac ggtgagcttg
       61 ccgtagagcg ccgcctgctg gggcacccag ccgatctggc gcgggcccag gctgacgctt
      121 ccgccgtcgg gctgctggat cccggccagg atcgacagaa gggtcgtctt gcccgcgccg
      181 ttgggcccga tcaccgccac cagctcgccc cgcgccgcgg acagcgacac ctcgcgcagc
      241 gccacgcggt cgccgtagcg cttggtcagt ccctcggccg aaagcacggc gtcgccggtc
      301 acgcgccgca gagtggcaga ctggccacgg tgaaggtcct gttcgtgggc gacgtggtgg
      361 gcggcatcgg ccggcggacg ctggccgcgc tgctacccgg gctgcgtgag cgccacgcgc
      421 ccgacttcgt ggtggtcaac ggcgagaacg cggccggcgg cgtcggcatc acccagaaga
      481 cggcgcgcca ggtcctggac atgggcgtcg acgtgatcac gctgggcaac cacgcctacc
      541 ggcacaccga ggtgtacgag ttcctggaca ccgaggagcg gatcgtgcgc ccggccaact
      601 acccgcgcgc cagccccggc cgcggcgtca ccgtggtcga gagggacggg gtggccctgg
      661 gcgtggtcaa cctgtccggc tctctgttcg tggaggcggc ccggtcggcg ttcgccgagg
      721 tggacgccgc gctgtccgac ctgcgcggcc gcgccaccca tgtgctggtg gacatgcacg
      781 ccgaggccac gagcgagaag gtggccatgg gctggcacgt ggacggccgc gtgaccgcct
      841 gcgtgggcac ccacacccac atccccaccg cggactgccg ggtcctcccc ggtggcacgg
      901 cctactgcac ggacgtcggc atgacgggcc cgcgcggcgg cgtgatcggg gtgaagaggg
      961 agtcggccct ggagcggttc cggaccatga ccaacgtgcg cttcgagacc tcggaccagg
     1021 acccgtggat caacgcggtg ctgatcgagg ccggcgcgga cgggaaggcc acggcgatcc
     1081 accagatcct cgagcccgct cccccaggga ccacatagcc acccccacga cgccgagcag
     1141 cgccaggtcg cggacgacca ccagggccag cgcgccggcg tcgcggtcga ccacctcgcg
     1201 gtagagcccc gggaactcga acagcgtcag caccacggcc agcgccgaca gcgcggccag
     1261 cgcccgcatc cgccaggcca gggccagggc cagcagcggc atcacccaga tcaggaactg
     1321 gggcgacagg accttgccca ggcaggcgaa cgccaccgtg gccgccagcg aggccagcac
     1381 gagcgcgcgc tcgtccggag cccgcgccac gccggtggcc agcaccgcca ccgccgcggc
     1441 caggagcgcg gtgaacaggg ccgccaccag gtcgccggcc ggatggtcca ggccgtcgga
     1501 gcggaagccg tcgttgggcg tggcggcgcc cagccccagc gagtcgaggg cacgcaccac
     1561 gatcgccggc gtgctctcca cctgcacggg gcggtccagg tggtaggtga acgagtccgc
     1621 gacgccggcc ggcgacagcg tggcgaccgc gccgtaggcc acgaccacga ccagggccag
     1681 cgcggccgcg ccctcgaccg cggcccggcg ctcgtcgcgc gccacgagcc aggccagcgc
     1741 gaccggcgcc accaccagcg ggtcgagctt gaccaccacc gccaccccca gcacggccat
     1801 gcccgctcgc gggcgcgcag cgcacaggag cgccagggcg gccagcgtca gcaccaccgg
     1861 caccaggtcg aagtgggtgc gcagcatcgc gccgcacagc acgggcgcag cggctgcgcc
     1921 caggagcgcc gcggtccgat cgccacccgt ggccgtggcg agccggccac acagcgccac
     1981 catggccacc gccagcgcga cggccaggac cgcgaacgcg atccggtagg cgtcctggtc
     2041 gccggccagg cccgcgaccg tgagcacggg ccaggccagc ggcgggtact cgaaggcgac
     2101 gtcccggtag ggcaggtgcc cgtcgaggaa gatctgcgcg taggaggagt acagcggcag
     2161 gtcggagatc gactcgtcgt cccaggggcc gaccaggaac gtcagcgcga agcccgccgc
     2221 cagcagcgcc gccgcgcccg ccgcggccct catccgggtg cggacggctc gggcccggtc
     2281 cgccaggggg ccatcagcgc caccagcgcc agcggcgcga accacacgat gtagaggtag
     2341 aaccagtggc tgaccgtcaa ctggaccgcg atcaccacgg cagcgcccag cgccgcgacc
     2401 tggaacgcgt cgcgtcgacg cggcacgaag aacaccgcga ccgccaggag cccggcgccc
     2461 gccttgagc
//
LOCUS       DASUPW010000528         4084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1783627, whole genome shotgun sequence.
ACCESSION   DASUPW010000528 DASUPW010000000
VERSION     DASUPW010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783627"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1199)
                     /locus_tag="VFY44_11870"
     CDS             complement(135..1199)
                     /locus_tag="VFY44_11870"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5911182.1"
                     /translation="MADDARRREIAELLQSLGLPEEAVRRAVERGSPEDAVFDAVLMP
                     EIAERTLSARQVSDAEGPPLELMHMFWEAFGLPIADDDEPAFSQEEARIFQLLYQMQE
                     TWPPEVGLQVARVYGRLLARIAQTEVQLFRLYIEPRLRDDDDPYASLAATRDAFAQLL
                     PVTGPLLLGVHRRWLEHHIAQEVVREAETEAGGLPGAVTVCFLFCDLKDFTAFAAERG
                     DQAAVTAIDRFAEVISRERGPQVRFTKGLGDGYMLVYADVPTALAVGRRIVTAMQDPE
                     VPSVHASAHQGTAIAREGDYFGSAVNLAARLLDVARGDELVVTATTAAADPDGGDWES
                     AGSVRVRGVQGPVDAVRQRL"
     gene            1209..2246
                     /locus_tag="VFY44_11875"
     CDS             1209..2246
                     /locus_tag="VFY44_11875"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEX5911183.1"
                     /translation="MPRTLTAGELNRALLARQMLLERRAVGLPKALERMAGLQAQYAP
                     SMYVGLWTRVRDLDRAAVTRALEQRRVVQATLMRATIHLVSRADYWPLELAVRDARRD
                     WAMRVMTGAPSARRMRTVAARARSLLADGPLSRAELEAELGKPEFRLLGMWLDLVRVP
                     PSGTWERRRADLLGLAEWWAGAPPEGCDEATGTVLLVKRYLAAFGPATRHEVADFCGL
                     SLERVDAALGAVDLRAFRAEDGADLVDLPRAPLPPADTPAPVRFLPTWDATLLVHARR
                     TQILPERHRGRIFNTRMPQSVGTFLVDGAVAGAWRPRDGRVEIEPFEQLTRAQRREVD
                     AEAERLLGFLV"
     gene            complement(2226..2693)
                     /locus_tag="VFY44_11880"
     CDS             complement(2226..2693)
                     /locus_tag="VFY44_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911184.1"
                     /translation="MNARIPAVALAALALLALPMPASAAKHPRSAVREFTGTVASVSG
                     KKKTFRLRRTGRAAIVVQLSRKTKVAKGAKPLKGRKLVVKARRAGKGRSWLARSVKLV
                     PVAAEDEPAEDDVLDEEPVTEDEEGEDTDPVLDLEDTLGDLFGDDEGSDEEPE"
     gene            complement(2757..>4084)
                     /locus_tag="VFY44_11885"
     CDS             complement(2757..>4084)
                     /locus_tag="VFY44_11885"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5911185.1"
                     /translation="WRARHGTRERRPWLALTLATGCWTVGAVYHLLAHGGGSTTAVPT
                     GLDAVGYLLFYPLAFAGLAGLMRSRLRTHATLPWLDGAIAGVGTAAAALALVGGLLAE
                     RAGDSPAELLAKIGFPIGDLVLLALVAVMWGLRGMRGRRSWYLVSGGVGVMAAADLSF
                     TAFAAQSDLAVQVLRPMWGVGMILIGLGAWRGEPEEADVHPEGGQLVLPWLFAGVAVT
                     VLLAGQSGDVPVAAAALAALTVVAVMFRATAVLRENKLLLDARQQAVTDDLTGLPNRR
                     MFNALLRRLDLGGGRPVGILMMDLNRFKQLNDALGHRAGDQLLQELAPRLRQAAGDGG
                     TVGRLGGDEFAMVVEGADEALLAAVADAVLRAIRRPFVIEGLTLQVDASVGGALAPGH
                     ADDPSTLLRCADVAMYAAKRDGAGYALYRPGTDHEGAHDHDRLRLVEPA"
BASE COUNT          500 a         1538 c         1551 g          495 t
ORIGIN      
        1 cctgaccgag tgcatcggct gcggctgcct gtcgctggcc cggtgcaagc tggccaaccc
       61 ggacgacggc gttgccagca ccggcagcgg gccgcgctac tgggtcggcg agccgcgccc
      121 ggccgcctga gcgctcacag ccgctggcgc accgcgtcga cgggcccctg gacgccccgc
      181 acgcgcaccg agcccgcgga ctcccagtcg ccgccgtccg gatcggcggc ggcagtggtg
      241 gccgtgacca ccagctcgtc cccccgggcc acgtccagca gccgggcggc caggttcacg
      301 gcgctgccga agtagtcgcc ctcgcgcgcg atcgccgtgc cctggtgggc gctggcgtgc
      361 accgagggga cctcggggtc ctgcatggcg gtgacgatcc ggcggccgac ggccagggcg
      421 gtgggcacgt cggcgtacac cagcatgtag ccgtcgccca ggcccttggt gaaccgcacc
      481 tggggcccgc gctcgcggct gatcacctcg gcgaagcggt cgatggccgt gaccgccgcc
      541 tggtcgccac gctcggcggc gaacgcggtg aagtccttga ggtcgcagaa caggaagcac
      601 acggtgaccg ccccgggcag cccgccggcc tcggtctcgg cctcgcgcac cacctcctgc
      661 gcgatgtggt gctccagcca gcgccggtgc acgcccagca gcagcggccc ggtgacgggc
      721 agcagctgcg cgaaggcgtc gcgggtggcc gccagcgagg cgtacgggtc gtcgtcgtcg
      781 cgcaggcggg gctcgatgta cagccggaag agctgcacct cggtctgcgc gatccgggcc
      841 agcaggcgcc cgtagacgcg cgccacctgg agcccgacct cgggcggcca ggtctcctgc
      901 atctggtaga gcagttggaa gatgcgcgcc tcttcctggg agaacgccgg ctcgtcgtcg
      961 tcggcgatcg gcaggccgaa cgcctcccag aacatgtgca tcagctccag cggcgggccc
     1021 tcggcgtcgc tgacctggcg cgcgctgagc gtccgctctg cgatctcggg catcagcacc
     1081 gcgtcgaaca cggcgtcctc gggtgagccg cgctcgacgg cccggcgcac cgcctcctcg
     1141 ggcaggccga ggctctgcag cagctccgcg atctcccgcc ggcgcgcgtc gtcggccatg
     1201 gacacatcat gccgcggacg ctgaccgcgg gcgagctgaa ccgcgcgttg ctggcgcgcc
     1261 agatgctgct ggagcggcgc gcggtcggcc tgccgaaggc gctggagcgc atggccgggc
     1321 tccaggcgca gtacgcgccc tcgatgtacg tggggctgtg gacgcgggtg cgcgacctgg
     1381 accgcgcggc ggtcacgcgg gcgctggagc agcggcgcgt ggtgcaggcc acgctgatgc
     1441 gggcgaccat ccacctggtc tcgcgcgcgg actactggcc gctggagctg gccgtgcgcg
     1501 acgcccgccg cgactgggcc atgcgcgtga tgaccggcgc cccgagcgcg cgccggatgc
     1561 gcaccgtggc cgcgcgggcg cgatcgctgc tggccgacgg gccgctgtcg cgcgccgagc
     1621 tggaggccga gctgggcaag cccgagttcc ggctgctggg catgtggctg gacctggtgc
     1681 gggtgccgcc gtcgggcacg tgggagcgac gccgcgccga cctgctcggc ctggccgagt
     1741 ggtgggccgg cgcgccgccc gaggggtgcg acgaggccac cggaaccgtg ctgctggtga
     1801 agcgctacct ggccgcgttc gggccggcga cccggcacga ggtggccgac ttctgcggcc
     1861 tgtcgctgga gcgcgtggac gcggcgctgg gcgccgtgga cctgcgcgcc ttccgcgcgg
     1921 aggacggcgc cgatctcgtg gacctgcccc gggcaccgct gccgccggcg gacacgcccg
     1981 cgccggtgcg cttcctgccc acctgggacg cgacgctgct ggtgcacgcg cggcgcacgc
     2041 agatcctgcc cgagcgccac cgcggccgga tcttcaacac gaggatgccg cagtcggtgg
     2101 gcacgttcct ggtggacggc gccgtggcgg gcgcgtggcg cccgcgcgac gggcgggtcg
     2161 agatcgagcc gttcgagcag ctgacccgcg cgcagcggcg cgaggtcgac gcggaagccg
     2221 agcggctact cgggttcctc gtctgagccc tcgtcgtcgc cgaagaggtc gccgagggtg
     2281 tcctcgaggt cgagcacggg gtcggtgtcc tcgccctcct cgtcctcggt gaccggctcc
     2341 tcgtccagga cgtcgtcctc cgcgggctcg tcctcggcgg ccacggggac gagcttgacg
     2401 ctgcgcgcca gccagctgcg gcccttgccc gcgcgacggg ccttgaccac gagcttgcgg
     2461 cccttcagcg gcttggcgcc cttggccacc ttcgtcttgc gcgagagctg caccacgatc
     2521 gcggcgcggc cggtgcggcg caggcggaag gtcttcttct tcccggacac cgaggccacg
     2581 gtgccggtga actcccgcac ggcggacctg gggtgcttcg ccgccgacgc gggcatgggc
     2641 agggccagga gggcgagggc ggcgagcgcg accgcgggga tccgggcgtt catggccacg
     2701 gcatcggccc ggcggcccgc cggcttgcgc gcgagcccgc gaacctggac ggacggtcag
     2761 gcgggctcga ccagccgtag ccggtcgtgg tcgtgcgccc cttcgtggtc ggtgcccggc
     2821 cggtacaggg cgtagcccgc gccgtcgcgc ttggcggcgt acatggccac gtcggcgcag
     2881 cgcaggagcg tggacgggtc gtccgcgtgg cccggggcca gcgcgccgcc gacgctggcg
     2941 tccacctgca gcgtcaggcc ctcgatcacg aacggccggc ggatggcgcg caggaccgcg
     3001 tcggccacag cggccagcag cgcctcgtcg gcgccctcca cgaccatggc gaactcgtcg
     3061 ccgcccagcc ggccgacggt cccgccgtcc ccggcggcct ggcgcaaccg cggcgccagc
     3121 tcctgcagga gctggtcgcc ggcccggtgg ccgagcgcgt cgttgagctg cttgaagcgg
     3181 ttcaggtcca tcatcaggat ccccaccggg cggccgccgc ccaggtcgag gcggcgcagc
     3241 agcgcgttga acatgcgccg gttgggcagg ccggtcaggt cgtcggtcac ggcctgttgg
     3301 cgcgcgtcca gcagcagctt gttctcgcgc agcaccgcgg tggcgcggaa catgaccgcc
     3361 accacggtca gagcggccag cgccgccgcg gccacgggca cgtcgcccga ctgccccgcc
     3421 agcagcacgg tcaccgccac cccggcgaac agccacggca gcaccagctg gccgccctcg
     3481 ggatggacgt cggcctcctc gggctcgccc cgccaggcgc ccaggccgat caggatcatg
     3541 cccacgcccc acatgggccg cagcacctgc acggccaggt cggactgggc ggcgaaggcg
     3601 gtgaacgaca ggtcggccgc ggccatgacg ccgacgccgc cgctgaccag gtaccacgag
     3661 cgccggcccc gcatgccgcg caggccccac atcacggcca ccagcgccag cagcaccagg
     3721 tcgccgatcg ggaagccgat cttggccagc agctcggccg ggctgtcgcc cgcgcgctcg
     3781 gccagcagtc cgccgacgag cgccagggcg gcggccgccg tgcccacgcc cgcgatggcg
     3841 ccgtccagcc acggcagcgt ggcgtgggtg cgcagccgcg agcgcatcag ccccgccagc
     3901 ccggcgaagg ccagcgggta gaagagcagg tagcccacgg cgtccaggcc ggtgggcacc
     3961 gcggtcgtgg acccgccgcc gtgcgccagc aggtggtaca cggcgccgac ggtccagcag
     4021 ccggtggcca gcgtcagcgc cagccagggc cgtcgctcgc gggtgccgtg gcgcgcgcgc
     4081 cacc
//
LOCUS       DASUPW010000529         5337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_537110, whole genome shotgun sequence.
ACCESSION   DASUPW010000529 DASUPW010000000
VERSION     DASUPW010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537110"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VFY44_11890"
     CDS             <1..230
                     /locus_tag="VFY44_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911186.1"
                     /translation="SIGAATPPGVQGRSLLDPKGDPARPIRVLGTSGDEVGTTLPALL
                     RARERTLSVQRREVIGSPAWRAACRVPGSGC"
     gene            complement(227..1825)
                     /gene="murJ"
                     /locus_tag="VFY44_11895"
     CDS             complement(227..1825)
                     /gene="murJ"
                     /locus_tag="VFY44_11895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX5911187.1"
                     /translation="MAAETSEETASQRGGRLARSTAFFSIATGLSRVLGLVREVVAAS
                     YFGVSGAMSAFTIAFQVPNLIRALFADSALQGAFVPVFTELLEKGKKKEAFHVASSLF
                     FLITLVLGAITALFIIFAEPLMSVFAPGFDDNPALKDLTVGLTQLMFPIVLLLALSGV
                     VVGMLNSFEHFSVPALAPTAWNLVIIAALVGLTPLFEGQDQVYAYAIGVLVGTVVQFV
                     LPLPWLRGRGGHFSLALDWRNPHVLRVLKLMFPVTIALGMINLSLLINSLLGTLVSDQ
                     APAAIDKAFRIYQLPQGLFSISIATILFPTMSKLAARGATDDLRSTMSNGVRQVALLL
                     IPSAVLMAVLAEPITRLVYQRGEFDAEATELVKTAMVWWSISLPFQGISLLFSRTFFS
                     LQQPWATTALAFVNMIVNGLVSLALYKPFGIAGIVIGTVVGTLVMTTAQGWLLRGQLG
                     GIEGGKMLSSGLRMLAASALLAGAAYGAWDLLDALLGQSFPAQLVSVGVGIGAGLAVY
                     GAGVWALRVPEARQIRALLPGGGR"
     gene            1885..2148
                     /gene="rpsT"
                     /locus_tag="VFY44_11900"
     CDS             1885..2148
                     /gene="rpsT"
                     /locus_tag="VFY44_11900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX5911188.1"
                     /translation="MANIHSQVKRNLRTERERQENRRYSSSVKTYFRHLEEAADAKDA
                     DKAQAAYRELSSRIDRAARTGVFHKNTAARKKSRAARLLASAK"
     gene            2200..3708
                     /locus_tag="VFY44_11905"
     CDS             2200..3708
                     /locus_tag="VFY44_11905"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5911189.1"
                     /translation="MAGRDRGFAALIVGAMETRLLIGGQQVDGLGPSLAVENPATQET
                     VADVRSASPEQVDAAVAAAREGFAGWAGTPALERGEMLHEVATRLRAAKDELARVMTL
                     EGGKPFVENADEVDWTAAAFDFYAELGRASAGRVIPPIESTQLALVTKEPMGVVGAIV
                     PWNYPLLLLSWKLAPALAAGNTVVAKPSEVTPLSTLMLAPAFDHLPAGALSLLAGAGD
                     VGGAIVADERVDCVAFTGSVATGKKIASVCAERVARMNLEMGGKDPFIVCPDVAGDIE
                     IAARGGAWAAYLNAGQVCTSAERFYVHESVFDDYVAAFVDHTNTLNVGDPFDPATDLG
                     PMVSAPQRDKVVAQVEAAVGAGAELLTGGDRAGHEAGHFYAPAVVTGLPAETDLLREE
                     TFGPVAPIVPVKSLDEAIALANGTRFGLGANVYTRDLATAVRCMRELRAGTVWINDPL
                     TDNDAGPFGGFKQSGLGRELGQEGLEAFQETKHVHMETKIERKDWWYPYGPA"
     gene            3737..4102
                     /locus_tag="VFY44_11910"
     CDS             3737..4102
                     /locus_tag="VFY44_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911190.1"
                     /translation="MTQLPFGGIGRGVNNAARGAVGGVDRLISAVEGLAGGIASIEHE
                     MKGMRRDMREVIDSVEGLRRDLSGFDDIRTATTSMDREVQAMHQSLDGVNHLLDRYPR
                     LLGRSAAEKRARRNETKPS"
     gene            4099..>5337
                     /locus_tag="VFY44_11915"
     CDS             4099..>5337
                     /locus_tag="VFY44_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911191.1"
                     /translation="MNTGFFLPLFHNELVNMITIPIFSAVIGYTTNWTGVWMLFYPVR
                     FAGFRLPGLQALAGLLPRKLQQIPGVMQGGVGWQGIIPSRAAKMGSIAVDKGIAKVGG
                     PAEFYEQLEPDVIAEHILQSSRTDIRDVVERTMQREHPQLWNDLPPQLREAVHTRVQN
                     ELPAIVHDVTDEIGDNIDQLLDIKLMVIRHLEAQPALANRIFQETGRQELRFIINFGF
                     FFGFVLGLPTVAIVHFLPYWWVLPVLGTLVGWATNWLAIRMIFEPMEPRKIGPWTWQG
                     LFLKRQREAADVYAEVIADDIVTLRNIGQELLNGPRADRTRQMIHTALRPAVDRAVGP
                     ARAAVRVAVGTQEYDAIRESVATEGVGYTMTPLTDKDFNRRQSEKVRKLISARMREMA
                     PEDFSEMLRSAMREDEWLLIL"
BASE COUNT          865 a         1895 c         1870 g          707 t
ORIGIN      
        1 actccatcgg cgcggcgacg ccccccgggg tgcagggccg ctcgctgctg gaccccaagg
       61 gcgatcccgc gcgccccata cgcgtgctgg gcacgagcgg cgacgaggtc gggaccacgc
      121 tccccgccct gctgcgcgcc cgcgagcgca cgctgtccgt gcagcggcgc gaggtcatcg
      181 gctcgcccgc ctggcgcgcc gcgtgtcgcg tgcccggcag cggctgctag cgcccgccgc
      241 ccggcagcag cgcccggatc tggcgtgcct cgggcacgcg cagcgcccac acgccggccc
      301 cgtacacggc caggccggcg ccgatcccca cccccacgga gaccagctgg gccgggaacg
      361 actggcccag gagcgcgtcg aggaggtccc acgcgccgta ggccgcaccc gccagcaggg
      421 ccgaggcggc gagcatccgc aggccgctgc tcagcatctt gccgccctcg atcccaccga
      481 gctggccgcg cagcagccag ccctgggcgg tggtcatgac cagggtgccc accacggtgc
      541 cgatgacgat gccggcgatg ccgaacggct tgtacagggc cagcgacacc agcccgttca
      601 cgatcatgtt cacgaacgcc agcgcggtgg tggcccacgg ctgctggagg ctgaagaagg
      661 tgcgcgagaa cagcaggctg atgccctgga agggcaggga gatcgaccac cagaccatgg
      721 ccgtcttgac cagctcggtg gcctcggcgt cgaactcgcc gcgctggtag accagccgcg
      781 tgatcggctc ggccaggacc gccatgagca cggcgctggg gatcagcagc agcgcgacct
      841 ggcggacgcc gttggacatc gtcgagcgca ggtcgtcggt ggcgccgcgc gcggccagct
      901 tcgacatggt cgggaacagg atcgtcgcga tcgagatcga gaacaggccc tgcgggagct
      961 ggtagatccg gaacgccttg tcgatggccg ccggcgcctg gtcggacacc agcgtgccca
     1021 gcagcgagtt gatcagcagg ctcaggttga tcatccccag cgcgatcgtc accgggaaca
     1081 tcagcttcag caccctgagc acatgcgggt tgcgccagtc cagcgccagc gagaagtgcc
     1141 cgccccgccc ccggagccag ggcagcggca agacgaactg gaccaccgtg cccaccagca
     1201 ccccgatcgc gtaggcgtag acctggtcct ggccctcgaa cagcggcgtc agccccacca
     1261 gcgcggcgat gatcaccagg ttccaggccg tgggcgccag cgccggcacg ctgaagtgct
     1321 cgaagctgtt gagcatcccc accaccacgc ccgacagcgc cagcagcagc acgatcggga
     1381 acatcagctg ggtcagcccg accgtcaggt ccttaagcgc cgggttgtcg tcgaagcccg
     1441 gggcgaacac gctcatcagc ggctcggcga agatgatgaa cagcgcggtg atcgcgccga
     1501 gcaccagcgt gatcaggaag aacaggctgg aggccacgtg gaaggcctcc ttcttcttgc
     1561 ccttctccag cagctcggtg aacaccggca cgaacgcgcc ctgcagggcc gagtccgcga
     1621 acagcgcccg gatcaggttg ggcacctgga aggcgatcgt gaaggccgac atggcgccgc
     1681 tgacgccgaa gtagctggcg gccaccacct cgcgcaccaa ccccaggacg cgcgacagcc
     1741 cggtggcgat ggagaagaac gccgtgctgc gcgccaaccg cccgccgcgc tggctcgcgg
     1801 tctcctcgga agtctcggcc gccacgactc ggctacacta cgcggccgcc gcgcgggatc
     1861 tgtcccgagc ggctcttttc tgacatggcc aacatccact cacaggtgaa gcgcaatctg
     1921 cgcaccgagc gcgagcgcca ggagaaccgg cgctacagct cgtcggtgaa gacctacttc
     1981 cgccacctgg aagaggccgc agacgccaag gatgccgaca aggcccaggc cgcgtaccgc
     2041 gagctgtcct cgcggatcga ccgtgccgcc cgcaccggcg tgttccacaa gaacaccgcc
     2101 gcccgcaaga agagccgcgc ggctcgcttg ctggcctccg cgaagtagtc gccccgggcg
     2161 ggcatcctgc ccgcccacct gagggggagc cggccatcca tggccggtcg agaccgcggc
     2221 ttcgccgctc ttatagtcgg cgccatggag acgcgactac tgatcggtgg acagcaggtc
     2281 gacggcctgg ggccctccct ggcggtcgag aacccggcca cgcaggagac ggtggccgac
     2341 gtccgctcgg cctcgcccga gcaggtggac gccgccgtgg ccgccgcccg cgaggggttc
     2401 gccggctggg ccggcacccc ggccctggag cgcggcgaga tgctgcacga ggtggccacc
     2461 cgcctgcgcg cggccaagga cgagctggcc cgggtgatga cgctggaggg cggcaagccg
     2521 ttcgtggaga acgccgacga ggtcgactgg accgcggccg cgttcgactt ctacgccgag
     2581 cttggccgcg cctctgcggg acgcgtgatc ccgcccatcg agtcgaccca gctggcgctg
     2641 gtgaccaagg agccgatggg cgtggtgggc gccatcgtgc cctggaacta cccgctgctg
     2701 ctgctgtcct ggaagctcgc ccccgccctg gcggcgggca acacggtggt ggccaagccg
     2761 tctgaggtca cgccgctgtc cacgctgatg ctggcgccgg cgttcgacca cctgcccgcc
     2821 ggcgcgctgt cgctgctggc cggcgccggc gacgtgggcg gcgccatcgt cgccgacgag
     2881 cgggtggact gcgtggcctt caccgggtcg gtggccaccg ggaagaagat cgcttcggtg
     2941 tgcgccgagc gggtggcgcg gatgaacctg gagatgggcg gcaaggaccc gttcatcgtg
     3001 tgtcccgacg tggccggcga catcgagatc gccgcccgcg gcggcgcctg ggcggcctac
     3061 ctgaacgccg ggcaggtgtg cacctcggcc gagcgcttct acgtgcacga gtcggtattc
     3121 gacgactacg tggccgcctt cgtggaccac acgaacacgc tgaacgtggg tgacccgttc
     3181 gaccccgcga ccgacctcgg cccgatggtc tcggcgcccc agcgcgacaa ggtggtggct
     3241 caggtcgagg cggccgtggg cgccggcgcg gagctgctga ccggcggcga ccgcgccggg
     3301 cacgaggccg gccatttcta cgcgccggcc gtggtcaccg gcctgcccgc cgagaccgac
     3361 ctgctgcgcg aggagacctt cggcccggtg gccccgatcg tccccgtgaa gtcgctggac
     3421 gaggccatcg ccctggccaa cggcacccgc ttcggcctgg gggccaacgt gtacacccgt
     3481 gacctggcga ccgccgtgcg ctgcatgcgc gagcttcgcg cgggcacggt ctggatcaac
     3541 gaccccctga ccgacaacga cgccgggccg ttcggcggct tcaagcaatc gggcctgggg
     3601 cgcgagctgg gccaggaggg cctcgaggcc ttccaggaga ccaagcacgt gcacatggag
     3661 acgaagatcg agcgcaagga ctggtggtac ccctacggac cggcctaggt aggctgccga
     3721 ccgtgaggga gacgcagtga cgcagttgcc gttcggaggg atcggacgcg gggtcaacaa
     3781 cgccgcccgc ggcgccgtcg gcggcgtgga ccggttgata tcggccgtcg agggcctggc
     3841 cggcgggatc gcgtcgatcg agcacgagat gaagggcatg cgccgcgaca tgcgcgaggt
     3901 gatcgattcc gtggagggcc tgcgccgcga cctgagcggg ttcgacgaca tccgcaccgc
     3961 caccacctcg atggaccgcg aggtgcaggc catgcaccag agcctggacg gcgtgaacca
     4021 cctgctcgac cgctacccgc ggctgctggg ccggtctgcg gcggagaagc gcgcccgccg
     4081 gaacgagacc aaaccctcgt gaacaccggc ttcttcctcc cgctgttcca caacgagctg
     4141 gtgaacatga tcaccatccc gatcttctcg gcggtgatcg gctacaccac caactggacc
     4201 ggggtgtgga tgctgttcta ccccgtcagg ttcgccggct tccggctgcc cggcctacag
     4261 gcgctggccg ggctgctgcc gcgcaagctc cagcagatcc cgggcgtgat gcagggcggc
     4321 gtgggctggc agggcatcat cccctcgcgc gcggcgaaga tggggtcgat cgccgtggac
     4381 aagggcatcg ccaaggtggg cggcccggcc gagttctacg agcagctcga gcccgacgtc
     4441 atcgccgagc acatcctgca gagctcgcgc accgacatcc gcgacgtggt ggagcgcacg
     4501 atgcagcgcg agcaccccca gctctggaac gacctgccgc cccagctccg cgaggccgtg
     4561 cacacgcggg tccagaacga gctgccggcg atcgtgcacg acgtgaccga cgagatcggc
     4621 gacaacatcg accagctgct ggacatcaag ctcatggtca tacgccacct ggaggcgcag
     4681 cccgcgctgg ccaaccggat cttccaggag accggccgcc aggagctgcg cttcatcatc
     4741 aacttcgggt tcttcttcgg gttcgtgctg ggcctgccca ccgtggcgat cgtgcacttc
     4801 ctgccctact ggtgggtgct gccggtgctg ggcacgctcg tgggctgggc caccaactgg
     4861 ctggcgatcc ggatgatctt cgagccgatg gagccgcgca agatcggccc atggacctgg
     4921 cagggactgt tcctcaagcg ccagcgcgag gccgcggacg tgtacgccga ggtgatcgcc
     4981 gacgacatcg tgaccctgcg caacatcggc caggagctgc tgaacggccc acgggcggac
     5041 cgcacgcggc agatgatcca taccgccctg cgccccgccg tcgaccgtgc ggtcggcccg
     5101 gcgcgggccg ccgtgcgggt ggccgtgggc acccaggagt acgacgcgat ccgcgagtcc
     5161 gtggccaccg agggcgtggg ctacaccatg accccgctga ccgacaagga cttcaaccgg
     5221 cgccagagcg agaaggtgcg caagctcata tcggcgagga tgcgcgagat ggccccggaa
     5281 gacttctccg agatgctgcg ctcggccatg cgcgaggacg aatggctgct gatcctg
//
LOCUS       DASUPW010000530         3031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1230788, whole genome shotgun sequence.
ACCESSION   DASUPW010000530 DASUPW010000000
VERSION     DASUPW010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230788"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /gene="cobA"
                     /locus_tag="VFY44_11920"
     CDS             complement(<1..815)
                     /gene="cobA"
                     /locus_tag="VFY44_11920"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEX5911192.1"
                     /translation="MSDGMVYLVGAGPGDPGLMTRRSLELIAGADAILYDRLIPADAM
                     RGARADADLVFVGKAPGDVAMSQEDINATLVELGRAGKRVVRLKGGDPFVFGRGGEEA
                     EELAAAGVPFEVVPGVTAGVAAPAYAGIPVTHRDTAAAVAFVTGHERPGKDESDLDWQ
                     ALAAFPGTLVLYMGVKNLPLIAEQLIAAGRPADQPVAVVRRGTLPDQQTATAPLEGIA
                     AEVETKGIKAPAITVIGDSAGLRGTLAWLERRPLHGRTVAVTRARAQASTLADR"
     gene            complement(826..1713)
                     /gene="hemC"
                     /locus_tag="VFY44_11925"
     CDS             complement(826..1713)
                     /gene="hemC"
                     /locus_tag="VFY44_11925"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX5911193.1"
                     /translation="MKLGTRGSALALTQAGLVRDRIAGLGHEVELVVIRTSGDERPAG
                     EPAPETSDKERFVKEIEEALLDGRIDLAVHSAKDVPGELPDGLSIVGVPERADPRDAL
                     CGATSLDELPDGAAVGTASVRRRSQLLAARPDLDVREARGNVDTRLRKLAAGDYDALV
                     LAAAGLARLERDEGTAVDAGVMLPAPGQGCLALEGRTGDEAVAEVAVELTDFAALVAL
                     TAERALVARLEATCDTPVGAFAQLDGGALVLHGYAGTPDGSTWIRDALQGPAKEPAAL
                     GAAVGERMLAAGARQVLGD"
     gene            complement(1722..>3031)
                     /gene="hemA"
                     /locus_tag="VFY44_11930"
     CDS             complement(1722..>3031)
                     /gene="hemA"
                     /locus_tag="VFY44_11930"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX5911194.1"
                     /translation="TAPLALRERLAMPDGRATSVLAELRADDSVHEAVVVSTCNRTEL
                     YLVGGDPVEAETKALAVLARQAGIRPTELTDSLYAMRDRDAIRHLFSVAAGLDSMIIG
                     EAEIQGQIKRAYELALVEGATGPISNRLFREALGAGKRVRTETGVGRAHVSVSSVAVS
                     LAARELGDLTAKRVLVIGAGETGELTARALRASGVQTVFVANRHYDRAIGLAQRFGGT
                     AIRFEDLPAELAETDIVVSSTASPHVIVDREDLELTMAERPGRPLVIIDIAVPRDFDP
                     AAREVPGVALYDMDDLQRAVARGRSVREAEAVRARSLVDEEVERFGRWLTSLEVVPTI
                     AALRDRGEEVVQTVLRENEHRWESLSPGDRERLTVMARAVVRRLLHEPTLRLKASAGD
                     EEGDAAYVHVQALRDLFGLDLPAPELEERVAEVTELEARRRRG"
BASE COUNT          346 a         1193 c         1115 g          377 t
ORIGIN      
        1 cggtcggcca gggtgctggc ctgcgcgcgc gctcgcgtga ccgcgaccgt gcgcccgtgc
       61 agcggccgtc gctccagcca ggccagcgtg ccgcgcagcc cggccgagtc gccgatcacg
      121 gtgatcgcgg gcgccttgat ccccttcgtc tcgacctcgg ccgcgatccc ctccagcggc
      181 gcggtggcgg tctgctggtc gggcagcgtg ccgcgccgga ccacggccac gggctggtcg
      241 gcgggccggc ccgcggcgat cagctgctcg gcgatcaggg gcaggttctt cacgcccatg
      301 tacagcacca gcgtccccgg gaaggcggcc agcgcctgcc agtccaggtc gctctcgtcc
      361 ttgccgggcc gctcgtggcc ggtcacgaac gccaccgctg ccgcggtgtc gcggtgcgtc
      421 acggggatcc ccgcgtaggc cggcgcggcg accccggcgg tcacgcccgg caccacctcg
      481 aagggcacgc cggccgccgc cagctcctcg gcctcctcgc cgccgcggcc gaacacgaac
      541 gggtcgccgc ccttgaggcg gaccacccgc ttgccggccc ggcccagctc gaccagcgtc
      601 gcgttgatgt cctcctggct catcgcgacg tcgccgggcg ccttgccgac gaagacgagg
      661 tcggcgtccg cgcgcgcccc gcgcatggcg tcggcgggga tcaggcggtc gtagaggatc
      721 gcgtcggcgc cggcgatcaa ctcgagcgac cgccgcgtca tcagccccgg gtcgccgggg
      781 cccgcgccca ccaggtagac cattccgtcg ctcacggcgg agacgctagt cgcccagcac
      841 ctggcgcgca ccggccgcca gcatgcgctc gccgaccgcc gcgcccagcg cggccggttc
      901 cttggccggg ccctggagcg cgtcgcggat ccacgtcgac ccgtccggcg tccccgcgta
      961 gccgtgcagc accagcgcgc cgccgtccag ctgcgcgaac gcgccgaccg gcgtgtcgca
     1021 cgtggcctcc aggcgcgcca ccagcgcgcg ctcggcggtc agggcgacca gggcggcgaa
     1081 gtccgtgagc tccacggcca cctcggcgac ggcctcgtcc ccggtgcgtc cctccagcgc
     1141 caggcagccc tgcccggggg cgggcagcat cacgccggcg tcgaccgcgg tgccctcgtc
     1201 gcgctccagc cgggccaggc cggcggccgc cagcaccagc gcgtcgtagt cgcccgcggc
     1261 cagcttgcgc agccgcgtgt ccacgttgcc gcgcgcctcg cgcacgtcca gatcggggcg
     1321 tgcggccagc agctgcgagc gccggcgcac gctggcggtg cccaccgccg cgccgtcggg
     1381 gagctcgtcc aggctggtgg cgccgcagag ggcgtcgcgg gggtcggccc gctcgggcac
     1441 gccgacgatg gacaggccgt cgggcagctc gcccggcacg tccttggccg agtgcacggc
     1501 caggtcgatc cgcccgtcca gcagcgcctc ctcgatctcc ttgacgaagc gctccttgtc
     1561 gctggtctcc ggcgcgggct cgcccgcggg ccgctcgtcg ccggaggtgc ggatgaccac
     1621 cagctcgacc tcgtggccca ggcccgcgat gcggtcacgc accaggccgg cctgcgtcag
     1681 cgccagcgcg ctgccccggg tccccagctt catcggatgg ctcagccgcg gcggcggcgc
     1741 gcctcgagct cggtgacctc ggccacccgc tcctccagct cgggcgcggg caggtccagc
     1801 ccgaacaggt cgcgcagcgc ctgcacgtgc acgtaggcgg cgtcgccctc ctcgtcgccg
     1861 gcggaggcct tcagccgcag ggtcggctcg tgcagcagcc gtcgcaccac ggcgcgggcc
     1921 atgaccgtca gccgctcgcg gtcacccggc gacagcgact cccagcggtg ctcgttctcg
     1981 cgcagcacgg tctgcacgac ctcctcgccc cgatcgcgca gcgcggcgat ggtgggcacc
     2041 acctccaggc tggtcagcca gcggccgaag cgctcgacct cctcgtccac cagggagcgc
     2101 gcgcggacgg cctcggcctc gcggacgctg cgcccgcggg ccaccgcgcg ctgcaggtcg
     2161 tccatgtcgt acagcgccac gcccggcacc tcgcgcgcgg ccgggtcgaa gtcgcggggg
     2221 acggcgatgt cgatgatcac cagcggccgg ccgggccgct cggccatggt cagctccagg
     2281 tcctcgcggt cgacgatcac gtgcggcgac gcggtcgagc tgaccacgat gtcggtctcg
     2341 gccagctccg cgggcaggtc ctcgaagcgg atggcggtgc cgccgaagcg ctgggccagg
     2401 ccgatcgcgc ggtcgtagtg gcggttggcc acgaacacgg tctgcacgcc gctggcgcgc
     2461 agggcgcgcg cggtcagctc gccggtctcg ccggcgccga tgaccaggac gcgcttggcc
     2521 gtcaggtcgc ccagctcgcg ggcggccagc gacacggcga cggacgacac cgacacgtgg
     2581 gcgcgcccga cgccggtctc ggtgcgcacg cgcttgccgg cgcccagggc ctcacggaac
     2641 aggcggttgc tgatcgggcc ggtggcgccc tcgaccagcg ccagctcgta ggcgcgcttg
     2701 atctggccct ggatctcggc ctcgccgatg atcatcgagt ccagccccgc ggccaccgag
     2761 aacaggtggc ggattgcgtc gcggtcgcgc atggcgtaca gcgagtcggt cagctcggtg
     2821 gggcggatgc cggcctggcg ggccagcacg gccagggcct tggtctcggc ctccaccggg
     2881 tcgccgccga cgaggtagag ctcggtgcgg ttgcaggtgg agaccacgac ggcctcgtgc
     2941 accgagtcgt cggcgcggag ctccgccagc acggacgtgg cgcggccgtc gggcatcgcc
     3001 agccgctcgc gcagcgccag gggggcggtg t
//
LOCUS       DASUPW010000531         2016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_537310, whole genome shotgun sequence.
ACCESSION   DASUPW010000531 DASUPW010000000
VERSION     DASUPW010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537310"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(628..1872)
                     /locus_tag="VFY44_11935"
     CDS             complement(628..1872)
                     /locus_tag="VFY44_11935"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5911195.1"
                     /translation="MALDDLASTEERVGGMVRTPPGIDVARARRDTPGAAKVAHLNNA
                     GAALPPAQVTDAVIAHLRREAALGGYEAAEAAADQVELTYAAVARLIGCRTDEVAVVE
                     NATRAWDMAFYGLPLAPGDRILTARAEYASNVIAFLQVARRTGAVVEVIENDESGQLS
                     VADLRHRLSHGKGPVKLVAITHVPTQGGLVNPAEEVGDVARAAGVPYLLDACQSIGQM
                     PVDVERIGCDMLSATGRKFLRGPRGTGFLYVRRDFLDRIEPPFLDLHAATWTAPDAYE
                     VRGDARRFENWETNYAAKIGLGVAVDYALGWGLQSIEARVTGLADRLRGALSAVPGVG
                     VHDQGSRRCGIVTFTIAGVPAQQVQQQLAEHGINVSVSLVDYARLDLTARGLPDLVRA
                     SVHYYNTEDELDQLIEALPLAG"
BASE COUNT          276 a          690 c          719 g          331 t
ORIGIN      
        1 gatcgttccc cgccaccgtt ccggctgcgg caaggcgcac aacgctcttg gctgatcggt
       61 catagcgtcc cgtcgctgtc ccactcgcac cgccgcgcag ggcgaggacc ggatcgtgga
      121 gctgcggcga gcggcgcgga ccggaggacc ggatcggcga cagttcaccg ttccggcccg
      181 gagggcacgg acatgcgacg ggctcccgcc gtgacacgac gtcgccgcgg tggtgcgggc
      241 tggtgcacga tgcgcttcgc cgtcgggcag ctggtcgtgt tcagtgtgtg tgggcttcgg
      301 cgtcggcgcc gcgctgcccg gcaacgccgg tctcatccgc atggtcgtag tcctcgccgc
      361 tatggccctt ctcggcaggt ggaactggta tctgcccggg tggctgaact ggctccccga
      421 gctccacgtc gagggcgacc acgggtcctt ggcacccacc gccgaagcgt ccatcgccga
      481 ggaaacccgc gaaccccgag ctccgtgctg gctgctgccc gcagccctgt cggtcaggcc
      541 tggtcggcgg ggctacggac acagcccacc gaggcggcaa gcggcggatc cccgctggtc
      601 gaaggcccgg gtgattgtat ggcgggttca gcctgcgagc ggcagcgctt cgatgagctg
      661 gtccagctcg tcctcggtgt tgtagtagtg gaccgacgct cggacaaggt cgggcagtcc
      721 ccgggccgtc aggtcgaggc gggcgtagtc gacgagggac acgctgacgt tgatgccgtg
      781 ttccgccagc tgttgctgga cctgttgcgc gggaacgccg gcgatcgtga acgtgacgat
      841 tccgcatcgg cggctgccct ggtcgtgcac gcccacgccg ggcaccgcgc tcagcgcgcc
      901 gcgcaggcga tcggccaggc cggtgacccg ggcctcgatg gactgcaggc cccacccgag
      961 ggcgtagtcg accgcgaccc cgagaccgat cttggcggcg tagttggttt cccagttctc
     1021 gaaccggcgt gcgtctccgc ggacctcgta cgcatccgga gctgtccagg tggccgcgtg
     1081 caggtccagg aacggcggct cgatccgatc gaggaaatcg cgacggacgt agaggaatcc
     1141 tgttcctcgc ggtccccgca ggaacttgcg gccagttgcg gagagcatgt cgcaaccgat
     1201 ccgctcgacg tcgacgggca tctgcccgat ggactggcag gcgtcgagca ggtacgggac
     1261 tccggctgcc cgggctacgt cgccgacctc ctctgccggg ttgaccagcc cgccctgggt
     1321 cggcacgtgc gtgatcgcga ccagcttgac cgggcccttg ccgtgactga gccggtggcg
     1381 caggtcggcg acggagagct gcccggactc gtcgttctcg atgacctcga ccacggcccc
     1441 ggtgcgtctg gccacctgga ggaaggcgat cacattgctg gcgtactccg cacgcgcggt
     1501 caggatccgg tcacccggcg ccagggggag accgtagaag gccatgtccc acgctcgagt
     1561 ggcgttctcg accacggcca cctcgtcggt ccggcagccg atcagacgag cgacggcagc
     1621 ataagtgagc tcgacctggt cggccgcagc ctccgccgcc tcgtagccac ccagggccgc
     1681 ctcgcgccgc aagtgagcga tcacggcgtc ggtgacctgg gcgggcggca gggccgcgcc
     1741 cgcgttgttc aggtgggcga ccttggcggc ccccggtgtg tcccggcgcg cccgcgccac
     1801 gtcgattccg ggaggcgttc ggaccatgcc gccaactcgt tcctcggtgc tggcaaggtc
     1861 gtctagtgcc atctcggctc ctccatccgt ggccggtgct gctgcacctg gggaagtgga
     1921 tgtgaccgtt cgctgggaac tattccacta aggtgcgcgc ccgccagttc tgctggccct
     1981 ctcgggggtc tgctgcagga acgggtgaca ggctgg
//
LOCUS       DASUPW010000532         4862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_814328, whole genome shotgun sequence.
ACCESSION   DASUPW010000532 DASUPW010000000
VERSION     DASUPW010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814328"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1215)
                     /locus_tag="VFY44_11940"
     CDS             complement(55..1215)
                     /locus_tag="VFY44_11940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911196.1"
                     /translation="MRRSLLAFSLAVLGSALGGAPAMATELVDNDKAQCPSAAHTTIQ
                     AGVDAASANEQVLVCNGVYREQVTIDKDGIDLVSKVTRGATIKSPVTALSGPEALVHV
                     DGGSNVKVRRFTIAGPGPGTVDSLRQGVLVENNNAAGVKEAQITDNLIQDIRDTEAAA
                     NEEGVGVRVGRAYANTSGRASVLRNTIIRYQRGGVVVDGDGSFAAVATNTITGLGLLT
                     SPVTPVPAQQGIQVGSGTAYSASPRLEAAADINSNRISLNQYSGGGGEASIGILVLGT
                     DLAKDPRSARVSRIQSNKVFKNDYGVYLLDVENHLIRSNRVYENGDSGTAAPDGGMGA
                     FETSPGAGNNNRFDRNDARTNDGLDCEDGTSPLNTWTGNRGVDDSPSTICAP"
     gene            complement(1390..1462)
                     /locus_tag="VFY44_11945"
     tRNA            complement(1390..1462)
                     /locus_tag="VFY44_11945"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1427..1429),aa:Thr,seq:cgt)
     gene            complement(1517..2182)
                     /locus_tag="VFY44_11950"
     CDS             complement(1517..2182)
                     /locus_tag="VFY44_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911197.1"
                     /translation="MKKYLRLPSPAMAVALLALFTALSASAYAATVITGKNVRNGSLT
                     GADIQSKSIAGTDIKDGALAARDIKAGSLTADRFAGGQLNPTKPTRWVLVNRAGEIEA
                     QSGGFSVTAAYPAGAANGNVYINAGEDLSDNGIAATIALENQYDLGGPATPNNGTNAA
                     ADSNPEFAGEISASRCQIPGVVECGPPAAKTTSNFVVSPRESDGQPTTGTNRKKFYVV
                     ITG"
     gene            2341..2670
                     /locus_tag="VFY44_11955"
     CDS             2341..2670
                     /locus_tag="VFY44_11955"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX5911198.1"
                     /translation="MERRPLPGGGTAYVARSPGQRLLGLMGLRALAPGCVLLFPGCDS
                     VHTAWMRVAIDVVFLDADGGVLAVRRSLRPWRVARCRGAAAVLECPAGGAEVLVSGRS
                     SRAATGA"
     gene            complement(2633..3562)
                     /locus_tag="VFY44_11960"
     CDS             complement(2633..3562)
                     /locus_tag="VFY44_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911199.1"
                     /translation="MAATTLLLLIPTAGHAQGTTRTACPGTFQVLHNDSIGTLRLSAG
                     AYQITVANPAALSCAKAAQDLAEFLQDYDGKLRRPWNVNTRAVAFQRGSDGQISFSLA
                     RVGGATPGGGGSVVPTPTANACPGFFDVLHNDHIGSLSVPKDAYRITLLSPGSLGCAT
                     ASRLFTSFLQDFDGKLPSPWVLNKTTATFTRRGSNTGFRIKPAVGPEPKPSNGGRYPA
                     RRQPGECPGTFRVLNNDRVAGLALPAGPYLTFVYRGTGVSCSEASQLFRTFLSGQNVP
                     RGYAVAPATGVFSKGKRPVFRVKPQSPRGSTAR"
     gene            complement(3655..4173)
                     /locus_tag="VFY44_11965"
     CDS             complement(3655..4173)
                     /locus_tag="VFY44_11965"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX5911200.1"
                     /translation="MHGHRGGYRRGGPPGFGPRGRRARRGAVKAAVLVLLAEEPRNGY
                     ALIQEIEARSEGAWRPSPGSIYPALAMMEDEGLIRSVASEDQRAYELTDRGREELAKR
                     GEDAPAPWEPARGDTSDDTVELFKTMRQVYMASRQITEAGTPEQIKQAEQALAQTRRT
                     LYGLLAEDPEDE"
     gene            complement(4344..>4862)
                     /locus_tag="VFY44_11970"
     CDS             complement(4344..>4862)
                     /locus_tag="VFY44_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911201.1"
                     /translation="LLASDEGGALLDDAEAMVRAALGVGEGDPSVALLARAKEGGDRA
                     EAEVEAARAARLEGEPKGRERTALERQFDDTVKRETRRAHTAVLDVCLTLTALMLRDL
                     ICLAEGAPEAALDPDRARSLAAAAGDRDTRRLRVAAERCEQARLALTVNVTEDLALEA
                     LGYRLAALVGPR"
BASE COUNT          591 a         1681 c         1828 g          762 t
ORIGIN      
        1 cggccccccg acgccggctg ccgagggcat cttcggcagc cagatggcgt ccgtctacgg
       61 cgcgcagatc gtcgacgggc tgtcgtcgac tccccggttg ccggtccacg tgttgagcgg
      121 cgaggtcccg tcctcgcaat cgagcccgtc gttcgtccgc gcgtcgttgc ggtcgaaccg
      181 gttgttgttc ccggcgccgg gcgaggtctc gaaggccccc atgccgccgt cgggagcggc
      241 cgtcccggag tctccgttct cgtagacccg gttcgacctg atcaggtggt tctcgacgtc
      301 cagcaggtac acgccgtagt cgttcttgaa caccttgttg gactggatcc gcgagacccg
      361 agcgctcctg gggtccttgg ccaggtcggt ccccaggacg aggatcccga tgctggcctc
      421 tccgccgccg cccgagtact ggttgagcga gatgcggttg gagttgatgt cggccgctgc
      481 ctcgagccgg ggcgacgccg agtacgcggt gccgctaccg acctggatcc cctgctgcgc
      541 gggcaccggg gtcaccggtg acgtgagcag cccgaggccc gtgatggtgt tggtggccac
      601 ggccgcgaac gagccgtctc cgtcgaccac gacaccgccc cgctggtagc ggatgatggt
      661 gttccgcagc acggaggccc gtccggacgt gttcgcgtac gcacgaccca cgcgcacgcc
      721 gacgccttcc tcgttcgccg ccgcctcggt gtcacggatg tcctggatca ggttgtccgt
      781 gatctgggcc tccttgaccc cggcggcgtt gttgttctcg accagcacgc cctgccgcag
      841 gctgtccacc gtgcccggcc cggggccggc gatggtgaac cgccgcacct tgacgttgct
      901 gccaccgtcg acgtggacca gggcctccgg gccgctcagg gcggtgaccg gcgacttgat
      961 cgtcgcgccg cgcgtgacct tcgagaccag gtcgatcccg tccttgtcga tcgtgacctg
     1021 ttcgcggtac acgccgttgc acacgagcac ctgctcgttg gccgacgccg cgtcgacgcc
     1081 ggcctggatg gtggtgtggg ccgcggacgg gcactgggcc ttgtcgttgt cgaccagctc
     1141 cgtggccatc gcgggtgcgc cgcccagcgc gcttccgagt actgcgagcg agaacgccag
     1201 caatgagcgt ctcatagtcc tacctcctag gaacgagtgg atacgtgatg ccctagaggt
     1261 gggagttcgt gctgctcccc ctgccccgtc gcgaacgtac acccggttct ggggaggcgc
     1321 aagaaaacgc caaaggtggg ccaggagctg acccggatga cccccaggag ggccctcacg
     1381 ggcaggaaaa gccgccgatc ggattcgaac cgatgacccc gtctttacga gagacgtgct
     1441 ctaccaactg agctacggcg gcgtgggcgg caccggagtc tagccccggc gccgcccccg
     1501 tcggggtctc gccgggctag ccggtgatga ccacgtagaa cttcttgcgg ttggtgcccg
     1561 tcgtgggctg gccgtcgctc tcgcgcgggc tgaccacgaa gttgctggtg gtcttcgcgg
     1621 ccggcggtcc gcactccacg acgccgggga tctggcagcg cgaggcggag atctcgcccg
     1681 cgaactcggg gttggagtcg gcggccgcgt tggtgccgtt gttcggcgtg gcggggccac
     1741 cgaggtcgta ctggttctcc agcgcgatgg tggcggcgat gccgttgtcg ctcaggtcct
     1801 cgcccgcgtt gatgtagacg ttgccgttgg ccgcgccggc cgggtaggcg gcggtcaccg
     1861 agaagccgcc cgactgcgcc tcgatctcgc ccgcgcggtt gaccagcacc cagcgggtgg
     1921 gcttggtcgg gttcagctgc ccgccggcga agcggtcggc ggtcagggag ccggccttga
     1981 tgtcgcgcgc ggccagggcg ccgtccttga tgtcggtgcc cgcgatggac ttcgactgga
     2041 tgtcggcccc ggtgagcgag ccgttcctga cgttcttgcc ggtgatcacg gtggcggcgt
     2101 acgcgctggc cgacagcgcg gtgaacagcg cgagcagggc gacggccatc gcgggcgacg
     2161 gcaggcggag gtacttcttc atcggggcat cctttggttg gtgacgaatg tcacctggtc
     2221 cttcgcagcc cccgccgcgg aggttcagca cccacgcgcc aacctggcga gggggcgaac
     2281 cgggcgccgc ccgcgcctgt accgggcatg caccgcgacg gaccgcgccg gctccggcgg
     2341 atggagcgcc gccccctgcc cgggggcggc acggcctacg tggcccggag ccccggccag
     2401 cggctgctgg ggctgatggg gctgcgcgcg ctggcgcccg ggtgcgtgct gctgttcccc
     2461 ggctgcgact ccgtgcacac cgcgtggatg cgcgtggcca tcgacgtcgt gttcctggac
     2521 gccgacggcg gcgtgctggc ggtcaggcga tcgctgcgcc cgtggcgggt ggcccgctgc
     2581 cgcggcgcgg cagcggtgct ggagtgcccg gcgggcggcg ccgaggtgct ggtcagcggg
     2641 cggtcgagcc gcgcggcgac tggggcttga cccggaagac cggccgcttg cccttgctga
     2701 acacgccggt ggcgggcgcg accgcgtagc cccggggcac gttctggccg ctcaggaacg
     2761 tgcggaacag ctgcgaggcc tcgctgcagc tgacgccggt gccccggtag acgaaggtca
     2821 ggtacgggcc ggcgggcagc gccaggccgg ccacgcggtc gttgttcagc acacggaagg
     2881 tgccggggca ctcgccgggc tggcgccggg ccgggtagcg gccgccgttg ctgggcttgg
     2941 gctcggggcc gacggccggc ttgatgcgga agccggtgtt cgagccccgg cgcgtgaacg
     3001 tggcggtggt cttgttcagg acccacggag agggcagctt gccgtcgaag tcctggagga
     3061 aggaggtgaa caggcgcgag gccgtcgcgc agcccagcga gcccgggctg agcagcgtga
     3121 tccggtacgc gtccttgggg accgagagcg agccgatgtg gtcgttgtgc agcacgtcga
     3181 agaagccggg gcacgcgttg gccgtgggcg tggggacgac gcttccgccg ccccccgggg
     3241 tggcgccgcc gacgcgggcc agcgagaacg agatctggcc gtcgctgccg cgctggaagg
     3301 cgaccgcgcg ggtgttcacg ttccacggcc ggcgcagctt gccgtcgtag tcctggagga
     3361 actcggccag gtcctgcgcc gccttggcgc acgagagggc ggccgggttg gccacggtga
     3421 tctggtacgc gccggcgctc aggcgcagcg tgccgatgct gtcgttgtgc agcacctgga
     3481 aggtgccggg acacgcggtg cgcgtcgtgc cctgcgcgtg acccgcggtc ggaatgagca
     3541 gaagcagtgt ggtcgcggcc acggcggcgg caagcgacga tcggatccgt cctctcggca
     3601 tcggcgcaag gtacagcaga agctccccgc gcgcgaggcg cggggagccg tgggtcattc
     3661 gtcctcgggg tcctcggcca gcagcccgta cagggtccgc cgggtctggg ccagggcctg
     3721 ctcggcctgc ttgatctgct ccggggtgcc ggcctcggtg atctgacgcg acgccatgta
     3781 gacctggcgc atcgtcttga acagctcgac ggtgtcgtcg gaggtgtcgc cgcgtgcggg
     3841 ctcccacggg gcgggagcgt cctcgccgcg cttggccagc tcctcgcggc cgcggtcggt
     3901 gagctcgtac gcgcgctggt cctcggaggc gacggacctg atcaggccct cgtcctccat
     3961 catcgccagg gcggggtaga tcgagccggg gctggggcgc caggcgccct cgctgcgggc
     4021 ctcgatctcc tggatcagcg cgtagccgtt gcgcggctcc tcggccagca ggaccagcac
     4081 cgccgccttg acggcaccgc ggcgcgcgcg gcggccgcgg gggccgaagc cgggcggtcc
     4141 gccgcggcgg tatccacccc tgtgcccgtg catggaccag tcctcccgag ggtcgaagtg
     4201 ggcgggcccg ccgtgtcggg gcccgcagtg tcgtgagtgt cgcatcggtt ctcctatcag
     4261 tgtatctcga tgctatcgcg atatatcgta acaggtcgcg tgcgaagtcc aacgcagacg
     4321 gcagtccgca caccatgcgc cgctcagcgg gggccgacga gcgcggccag gcggtagccc
     4381 agcgcctcga gcgccaggtc ctcggtcacg ttgaccgtca gggccaggcg ggcctgctcg
     4441 caccgttccg cggccacgcg caggcggcgc gtgtcgcggt cgccggccgc cgccgccagg
     4501 ctgcgggccc ggtcggggtc cagcgcggcc tcgggcgcgc cctcggccag gcagatcagg
     4561 tcgcgcagca tcagcgcggt cagggtgagg cagacgtcga gcacggcggt gtgcgcccgg
     4621 cgggtctcgc gcttgaccgt gtcgtcgaac tggcgctcca gcgccgtgcg ctcgcgtccc
     4681 ttcggctcgc cttcgagcct ggcggcgcgg gcggcctcga cctccgcctc cgcccggtcg
     4741 ccgccctcct tcgcccgggc caacagcgcc accgacgggt cgccctcccc gaccccgagc
     4801 gcggcgcgca ccatggcctc ggcgtcgtcc agcagggcgc cgccctcgtc ggaggccagc
     4861 ag
//
LOCUS       DASUPW010000533        11881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_537511, whole genome shotgun sequence.
ACCESSION   DASUPW010000533 DASUPW010000000
VERSION     DASUPW010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 11881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11881
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537511"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1399)
                     /locus_tag="VFY44_11975"
     CDS             complement(<1..1399)
                     /locus_tag="VFY44_11975"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5911202.1"
                     /translation="MSSTGHHLDERRLRRLLDVGRALVSELDPDAVLDQVLQVARELT
                     GARYAALGILDPGGTELERFITSGIDDQTRQAIGDLPRGRGLLGELIRHPETLRLDHL
                     DTHPMSYGFPPGHPPMTSFLGVPVMVRGQAYGNLYLTDREDGPFDATDEDTLAVLSEW
                     AGVAIANSRLHSDLEHQRDELERAVRGLEATTTIATAVGGETDLDRILELIVKRGRAL
                     VEARTVLILLAEGDELAVAACAGEVTDELASLRLPISGSVPGAVLRSGRSERMADVRN
                     RVPMAPVALAADARTELVVPLAYRGESSGVLVAFDRLRGGPEFDAEDERLLRSFASSA
                     ATAVATAQTVQADRLRHSIDAAEQERRRWARELHDETMQSLASLKMLLEAGVRRPGGA
                     EAVVAEASEQLGMEVEKLQALITELRPAALDDIGLASALVALVERSRITQGFEATAEI
                     DLDYDRGRAPDRLPME"
     gene            complement(1460..2575)
                     /locus_tag="VFY44_11980"
     CDS             complement(1460..2575)
                     /locus_tag="VFY44_11980"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5911203.1"
                     /translation="MNRFSVLIAGGGVAALEAVLRLRRLAGDQIDLTVLAPNEEFVYR
                     ALTVGEPFSRGAPEPHPVGPLLHHADAAWVRDSMTSVDVEDQVVHTAGGSRLAFDALL
                     IAVGARPKAVVPHARTFDDRHADEMLRGVVQDIEGGYVRRLAFVSPPGPVWPLPLYEL
                     ALMSAHAARDAGIDDMQIDLFTAESRPLPAFGPTVGSAVAGLLQDAGIRFHASSEPEV
                     AGTGRLVTPAGDELRVDSLVALPRLTGPPVKGIPLGDHGFIDVDEHCEVKGTGHRVYA
                     AGDAIAFPVKHGGVGAQAADVAAAAIANRAGLDVPLVPFHPVVHGKLLTGAGPKFLFT
                     RYVGGEGFDSVIADTPVGSTEKVTAAELTPYLQRNAA"
     gene            2706..3554
                     /locus_tag="VFY44_11985"
     CDS             2706..3554
                     /locus_tag="VFY44_11985"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5911204.1"
                     /translation="MTLLVGFDGSRQAAHAIEIAADLLPARDCRVVNMWSPPYFDDEL
                     RARLWPRSGDMDEFAAAVEQEGEAEARRLTAEGKALADAAGWAANVLTHRCYGDPGFE
                     LARLAQHEQAAAVVVGSRGLSGVRAMLGSTSDGLVHYSSVPVLVVPYPLLERERQAAA
                     SGPVLVGTDGSEGAATALAAADSLFGGRPAEVATVGFDEEGRGPVGPGAVSLRPEGVT
                     ASGRAIADALGHHARDIGASAIVVGSRGQAVHRELLLGSVAMAVLHHAHRPVLVVPNP
                     DKPDLP"
     gene            3593..4471
                     /locus_tag="VFY44_11990"
     CDS             3593..4471
                     /locus_tag="VFY44_11990"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5911205.1"
                     /translation="MYRKIISGHDGGEGGDDALALADALGRVTGAEVTVVGVFPDGPF
                     VDPEQQTAYARKVQAAADRAGVECDAFPAASPARGLHDAAEELDADLIVVGSRRRSTP
                     GHVSAGHVGLQLLHGSPCAVAVAPAGAREDELALREIGVAVDGSPESGLAQDAAVRLA
                     GETGAGVRLISVLNPEASAFGWGYGVIDLEEQMRAIYGERLTEAASRIPDGIEVEKEL
                     LSRGPAADLIEGAAKHVDLLVMGSRGYGPLRRVLLGSVSAPVVKHCPCPVLVVPRGVA
                     PAQDADDAAAVAEQTR"
     gene            4468..4932
                     /locus_tag="VFY44_11995"
     CDS             4468..4932
                     /locus_tag="VFY44_11995"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5911206.1"
                     /translation="MTVPAARSLVVGYDGSEPARAAVAYAADRVGPGGRVFIVHATGP
                     LASWLGAPEEMLVTEDRAEHGRAVLDELVLEAGNALLDTDYELVLVPGPAAEALVHTA
                     QRHEADEIVLGSRGMGRLGSVLGSVAQQVLHTADRPVVVIPYLAVREKAAGR"
     gene            4941..5903
                     /locus_tag="VFY44_12000"
     CDS             4941..5903
                     /locus_tag="VFY44_12000"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5911207.1"
                     /translation="MLLCYDGSRESANAITRAGELFGGGDATVVCVWSGLSSLMLHAP
                     LAGPPTGPLAEGSDMLDEPEHERAGRRAGEGAALARAAGFKARPLALKEERNVWHTLR
                     DHATDEAVTAMVVGARGLSRVSSVMLGSVSTGLVHHAPVPLLVVPPTADVRAPGPVVF
                     CDDGSEGARRAITTGRRLLRGPGLVLSVWRPWVVRAPYTAVGAGTAMGMAEELDDTAG
                     EHAATVADRGAEIARRAGGECATEAVRHDGPTWRGLIEVASDHRASAIVVGSRGLTGI
                     ASTLGSVSTAVAHHSPRPVMVVPPGAGSGAHHDGGDDQVDGDRQ"
     gene            complement(5850..6830)
                     /locus_tag="VFY44_12005"
     CDS             complement(5850..6830)
                     /locus_tag="VFY44_12005"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX5911208.1"
                     /translation="MGRVAAAAPRLGIARELATGRVPRSRPNRTAADVRAEVSGASFD
                     YAGAVAVLDGRRLVGVATIEDVLGAPGDALMADLMDRAPPTIGSATDEEAVVWETVMR
                     GEATVAVEGRFGEFRGLIPPSRLLGVLLHEHDEDLARLGGYLAGTVEARGAAQEGVAD
                     RLWHRIPWLLVGLAGAMASAVIVGGFEEQLEKTVLLAFFLPAVVYMADAVGTQTETVL
                     IRGLSVGVELRKVVWRELATGGVVGLLMAAAFLPFALLVFDDGHVAVAVGLALFASCS
                     IATLVAMVFPWLFHRFGADPAFGSGPLATVVQDLLSIAVYLVIATIVVGA"
     gene            complement(6818..8017)
                     /locus_tag="VFY44_12010"
     CDS             complement(6818..8017)
                     /locus_tag="VFY44_12010"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5911209.1"
                     /translation="MTEVAIAGAGVAGLEALIGLRALAGERVRITLVAPGPDFVYRPA
                     SVAEPFGQGRPRRYALAAIAEAFQAELVEDALERVDGRARCLHTTSGATIAYDALLLA
                     LGATPEPVWRHAVTFAGSADAQAVRAVVTAVEQGRVQSVAFVVPSGVAWSLPAYELAL
                     MTAKRARDAGQEPELVVYTPERQPLAVFGREAAEEVAGVLDQAGVRLARSVAAEGSPA
                     GELVVPFEETPIAYERVIALPRLAGPAVPGVPHDRDGFIPIDRHARVVGAQGLYAAGD
                     GTNFGIKQGGIAAAQADAAAEMIAAEAGVDIDPRPFRPTLKGKLLTGGTARFLRSDRG
                     PGAPASTAATSPLWWPQAKIAANYLAPYLAAQDAAAGLTESIQPAAGVQELPGLGGPF
                     EDSPWGE"
     gene            complement(8014..8568)
                     /locus_tag="VFY44_12015"
     CDS             complement(8014..8568)
                     /locus_tag="VFY44_12015"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEX5911210.1"
                     /translation="MPKLLVLYASTHGQTEKIATRIGDVLRGEGADVVTEKADGTVAT
                     DPSGFDAVVVGASVHAGHHQRDLRDWAKAHAPTLNGMPSAFFSVSLSAAEDADESRRE
                     NRKYVDDFAEQTGWTPTETSVIAGALQYLEYDFMTRLLMRLLMKRGHHPTDTSRDYEY
                     TDWDQVEAFARRCSAIAHTTHVGP"
     gene            8702..10087
                     /locus_tag="VFY44_12020"
     CDS             8702..10087
                     /locus_tag="VFY44_12020"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HEX5911211.1"
                     /translation="MATTSERVARGKAARAEAPRSSHAEFDPSGRPDPVALLEQQAAT
                     RVPELVPIRHGRMLASPFTFFRGAAKIMAHDLAGTPRSGLDVQCCGDAHLSNFGVFAS
                     PERKLVFDLNDFDETLPGPWEWDVKRLAASVLVAAGENGFKRKRQDRIVLETVRSYRT
                     AMAEFAGMGNLDVWYSHAEIENVLAEYAPQLAARAVKRTRKSLAKARTKDSTAAFAKL
                     TEVVDGQVRIADGSPLIVPVDKLARDTGRDETLDRLRELMDDYRDSLDPDRRALLASF
                     ELADVARKVVGVGSVGTRAWIALLFGRDQQDPLFLQLKEAEASVLEEFLAPSEYSNHG
                     QRVVAGQRLMQATSDIFLGWLHSEVGMDGRPRDFYGRQLKDWKGSAEIERMDAEGMAV
                     YGRLCGWALARAHARSGDRIAIAAYLGGSDTFDRAVLAFSRDYAEQNEADHRALGAAV
                     DSGRVAAQPGL"
     gene            complement(10092..10469)
                     /locus_tag="VFY44_12025"
     CDS             complement(10092..10469)
                     /locus_tag="VFY44_12025"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5911212.1"
                     /translation="MTEPTVDVAVLEAFLAAFNRHDADAVMEFFHEDCVLDMPRGPAP
                     GGRHLVGKEQVRQGIEARLAGIPDIHYGDDRHWVAGDRGASEWTVTGTDTDGRAVHVR
                     GCDLLEFRDGLISRKDSYWKLVE"
     gene            10624..11079
                     /locus_tag="VFY44_12030"
     CDS             10624..11079
                     /locus_tag="VFY44_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911213.1"
                     /translation="MSVRTTGEAPAPGRSTGAGPPAVSGDDAGYGWVAFAGVLLVIAG
                     MLNAIYGIAAIDDAQFFVGNTEFVFSNLNTWGWIVLCLGVAQLLIAFGVFAKNQFARW
                     TGVFVLGMNSIAQMLFIDAYPFLSLSILAMNLLAIYGLAAYGGRIATTR"
     gene            complement(11126..11398)
                     /locus_tag="VFY44_12035"
     CDS             complement(11126..11398)
                     /locus_tag="VFY44_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911214.1"
                     /translation="MLPRPLPNRSQALKTCALAATAALASTLLLIAATLAPAPAAALP
                     FLVAVCIGFPLAAAWELPPAMRALRAVSDMRRSLDELPETEHPLGY"
     gene            complement(11467..>11881)
                     /locus_tag="VFY44_12040"
     CDS             complement(11467..>11881)
                     /locus_tag="VFY44_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911215.1"
                     /translation="YRAPMRARDRDAPEGAGAAFGLEHGVVGIGDGQGEKAARMLDRF
                     ASLPEGTFVWTRGPDGSYHLGRITGPWRYDDSPAAREVGIHHVRPTRWLERPFGDREV
                     PAAVAQTFARGGRNFQRTHDDEAERQTAALWDSSA"
BASE COUNT         1595 a         4308 c         4440 g         1538 t
ORIGIN      
        1 cctccatcgg caaccggtcg ggggcgcggc cgcggtcgta gtccaggtcg atctcggccg
       61 tggcctcgaa cccctgtgtg atgcgcgagc gctcgaccag ggcgaccagc gccgacgcca
      121 ggccgatgtc gtccagcgcg gcgggacgca gctccgtgat cagggcctgc agcttctcga
      181 cctccatccc gagctgctcg gaggcctcgg cgaccaccgc ctcggccccg ccgggccgcc
      241 ggaccccggc ctccagcaac atcttcaggc tggccaggga ctgcatcgtc tcgtcgtgca
      301 gctcgcgggc ccagcgccgg cgctcctgct ccgcggcatc gatgctgtgg cgcagccggt
      361 cggcctgcac cgtctgcgcc gtggccacgg cggtggcggc gctggaggcg aaggagcgca
      421 ggagccgctc gtcctcggca tcgaactcgg ggccgccgcg caagcggtcg aacgcgacca
      481 ggacccccga ggactcgccg cggtacgcga gggggaccac cagctccgtc cgggcgtccg
      541 ccgccagcgc gacaggcgcc atgggcaccc ggttccggac gtcggccatc cgctccgagc
      601 gccccgagcg cagcacggca ccggggacgg agccgctgat gggcagccgc agcgacgcca
      661 gctcgtcggt gacctcgccg gcacaggccg ccaccgccag ctcgtcgcct tcggccagca
      721 ggatcagcac ggttcgcgcc tcgaccaggg cgcggccgcg cttgacgatc agctccagga
      781 tgcggtccag gtcggtctcg ccgcccaccg cggtggcgat ggtggtcgtg gcctcgaggc
      841 cgcggacggc gcgctccagc tcgtcgcgct gatgctcgag gtcggagtgc aggcgcgagt
      901 tggcgatggc gaccccggcc cactccgaca gcacggcgag cgtgtcctcg tcggtggcgt
      961 cgaacggccc gtcctcacgg tcggtcaggt acaggttgcc gtaggcctgg ccgcgcacca
     1021 tcacgggcac gcccaagaac gaggtcatcg gcgggtgccc gggcgggaac ccgtagctca
     1081 tgggatgggt gtccaggtgg tccagccgca acgtctccgg atgccggatc agctcaccca
     1141 gcaggccccg cccccggggc aggtcgccga tcgcctgccg ggtctggtcg tcgatcccgg
     1201 atgtgatgaa ccgctccagc tcggtgccgc cggggtcgag gatgcccagg gccgcgtacc
     1261 gggcgcccgt cagctcccgg gccacctgga gaacctggtc gagcaccgcg tccgggtcca
     1321 actccgacac cagcgcccgg cccacgtcca gcagccgccg caggcgccgc tcgtccaggt
     1381 ggtgaccggt cgaggacatg ggggcctccc aagctagccc gcgcggatac gcggggcaag
     1441 gcgcccgagg tgcgcgcccc tatgcggcgt tgcgctggag gtagggcgtc agctcggcgg
     1501 cggtcacctt ctcggtcgag cccaccggcg tgtcggcgat caccgagtcg aagccctcac
     1561 cgcccacgta ccgggtgaac aggaacttgg gcccggcacc ggtcaggagc ttgccgtgta
     1621 ccacagggtg gaacggaacc aggggcacgt ccaggccggc acggttggcg atcgcggccg
     1681 ccgcgacgtc cgcggcctgg gcgcccacgc cgccgtgctt gacggggaag gcgatggcgt
     1741 cgccggccgc gtagacgcgg tggccggtgc ccttcacctc gcagtgctcg tctacatcga
     1801 tgaacccgtg gtcgcccagc gggattccct tgacgggcgg gccggtcagc cgcggaagcg
     1861 cgaccaggct gtccaccctc agctcgtcgc ccgccggagt gaccaggcgg cccgtgccgg
     1921 cgacctccgg ctccgaggag gcgtgaaagc ggatccccgc gtcctgcagc agacccgcca
     1981 ccgcgctgcc gaccgtaggc ccgaacgcgg gcagcggccg gctctcggcc gtgaacaggt
     2041 cgatctgcat gtcgtcgatc cccgcgtcgc gggcggcgtg ggccgacatc agcgccagct
     2101 cgtagagggg cagcggccag accgggccgg ggggactgac gaaggcgagc cggcgcacgt
     2161 agccgccctc gatgtcctgg accacgccgc gcagcatctc gtcggcgtgg cggtcgtcga
     2221 acgtgcgggc gtggggcacg accgccttcg gtcgcgcgcc cacggcgatc agcaacgcgt
     2281 cgaaggcgag ccggctgccc ccggccgtgt gcacgacctg gtcctcgacg tcgaccgagg
     2341 tcatcgagtc ccgtacccac gccgcgtcgg cgtggtggag cagcgggccg accgggtggg
     2401 gctcgggcgc gccccgggag aagggctcgc ccacggtgag cgcgcggtag acgaactcct
     2461 cgttgggcgc gagcaccgtg aggtcgatct ggtcgccggc caggcggcgg agacggagca
     2521 cggcctccag ggcggcgacg cccccgccgg cgatgagtac ggagaagcgg ttcatgcgaa
     2581 ccaagctagg aagccacggc gggccgggca tccgggtcgg cccgccgatg gctgcggaga
     2641 actacgggcg ccggtccgca gcctcggccg atggccggcg gcggttaccg atctagcttg
     2701 gccccatgac gcttctggtc ggattcgacg gctcccggca agccgcccac gccatcgaga
     2761 tcgcggcgga tctgcttccc gcacgggact gccgggtggt gaacatgtgg tcgccgccct
     2821 acttcgacga cgagctccgc gcacgcctgt ggccccgtag cggggacatg gacgagttcg
     2881 cggcggcggt cgagcaggag ggcgaggccg aggcccgccg cctgaccgcc gagggcaagg
     2941 ccctggccga tgcggccggc tgggccgcca acgtgctgac gcaccgctgc tacggcgacc
     3001 ccgggttcga gctggcgcgg ctcgcgcagc atgagcaggc ggccgcggtg gtcgtcggct
     3061 ccaggggcct gagcggggtc agggcgatgc tcgggagcac gtccgacggc ctggtgcact
     3121 acagctcggt ccccgtcctg gtggtcccgt accccctgct cgagcgcgag cggcaggctg
     3181 cggcatccgg accggtgctc gtgggcacgg acgggtcgga gggagcggca accgcgctcg
     3241 ccgccgccga ctcactgttc gggggccggc cggccgaagt cgccaccgtc ggcttcgacg
     3301 aggagggccg cggcccggtc gggccgggcg ccgtgtcgct gcggcccgaa ggggtgacgg
     3361 ccagcggacg ggccatcgcc gacgcgctgg gccaccacgc ccgggacatc ggcgcctcgg
     3421 cgatcgtcgt gggctcgcgc ggccaggccg tgcaccgcga gctgctgctg ggcagcgtcg
     3481 ccatggccgt gctccatcac gcccaccggc ccgtgctggt ggtcccgaac ccggacaagc
     3541 ccgaccttcc ctgagccatg caccgcgaca ccccgacccg gaggacctag ccatgtaccg
     3601 gaagatcatc agcggacacg acggcggcga gggcggggac gacgcgctcg ccctggccga
     3661 tgccctcggg cgcgtgacgg gcgccgaggt gaccgtcgtc ggcgtcttcc ccgacggccc
     3721 gttcgtggac cccgaacagc agacggccta cgcccgcaag gtgcaggcgg ccgccgaccg
     3781 cgccggagtg gagtgcgacg ccttcccggc cgcgtctccc gcccgcggac tgcacgatgc
     3841 cgccgaggag ctggacgccg acctgatcgt ggtcgggtcc cggcggcggt cgaccccggg
     3901 gcacgtcagc gccggccacg tgggcctcca gcttctgcac ggctcgccgt gtgcggtggc
     3961 cgtcgcgccc gcgggagcgc gcgaggacga gctcgcgcta cgcgagatcg gcgtcgccgt
     4021 cgacgggtca cccgagtccg gcctggccca ggacgccgcc gtacggctgg ccggggagac
     4081 cggggccggc gtgcgcctga tctcggtcct gaacccggag gcgagcgcct tcggctgggg
     4141 ctacggggtc atcgacctgg aggagcagat gcgggcgatc tacggcgagc gcctgaccga
     4201 ggccgcttcc cgcatcccgg atgggatcga ggtcgagaag gagctgctct cgcgcggccc
     4261 ggccgccgac ctgatcgagg gggccgccaa gcacgtggac ctgctggtca tgggctcacg
     4321 gggctacggc ccactgcggc gcgtcctgct gggctcggtc tcggcgccgg tggtcaagca
     4381 ctgcccgtgc ccagtgctgg tcgtgccgcg tggcgtggcg cccgcacagg acgccgacga
     4441 cgccgcagcg gtggcggagc agacgcgatg accgtccccg cggcgcgctc gctggtcgtc
     4501 ggctacgacg gatcggagcc cgcgcgggcg gcggtggcgt acgccgccga ccgggtcggc
     4561 cccggcggcc gggtcttcat cgtccacgcg acgggcccgc tggccagctg gctcggcgcg
     4621 ccggaggaga tgctggtcac cgaggaccgc gccgagcacg gccgggccgt gctcgacgag
     4681 ctggtgctgg aggccggcaa cgcgctgctg gacaccgact acgagctggt gctggtgccc
     4741 gggcccgctg ccgaggccct ggtgcacacc gcgcagcggc acgaggccga cgagatcgtc
     4801 ctgggctcgc gcgggatggg gcgcctgggc agcgtgctgg gcagcgtggc ccagcaggtc
     4861 ctgcacacag cggatcgccc ggtggtggtc atcccctacc tggccgtgcg cgagaaggcg
     4921 gcgggccgct gatgggcggc gtgctgctct gctacgacgg ctcccgcgag agcgcgaacg
     4981 cgatcacccg tgccggagag ctgttcggcg gcggtgacgc gaccgtcgtc tgcgtgtggt
     5041 cggggctgtc ctctctgatg ctgcacgccc cgctggccgg cccgcccacg ggaccgctgg
     5101 ccgaggggtc ggacatgctc gacgagcccg agcacgagcg ggccgggcgg cgcgccggcg
     5161 agggagcggc cctggcgcgc gccgcaggct tcaaggcccg gccgctcgcc ctgaaggagg
     5221 agcgcaacgt ctggcacacc ctgcgcgacc acgcgaccga cgaggccgtg acggcgatgg
     5281 tggtcggcgc gcgtggcctg tctcgggtgt cgtcggtgat gctgggaagc gtctccaccg
     5341 ggttggtgca ccacgctccc gtcccgttgc tggtggtgcc cccgaccgcc gatgtccgcg
     5401 cgccggggcc cgtggtcttc tgtgacgacg gatcggaggg cgctcggcgc gccatcacca
     5461 ccgggcgccg gctgttgcgt gggccgggcc tcgtgctctc cgtctggcgg ccgtgggtcg
     5521 tacgcgcccc gtacacggcg gtcggggccg gcacggcgat gggcatggcc gaggagctgg
     5581 acgacaccgc gggcgagcac gccgcgaccg ttgccgaccg cggcgccgag atcgcgcgcc
     5641 gggccggcgg ggagtgcgcc accgaggcgg tccgccacga cggccccacc tggcgagggc
     5701 tgatcgaggt ggcaagcgac caccgcgcat ccgcgatcgt cgtcggatcg cgcggcctga
     5761 cgggcatcgc cagcaccctg ggcagcgtct cgaccgccgt cgcgcaccac agcccgcggc
     5821 cggtgatggt ggtgcccccg ggcgccgggt caggcgccca ccacgatggt ggcgatgacc
     5881 aggtagacgg cgatcgacag taggtcctgg accacggtgg ccagcggccc ggatccgaac
     5941 gccggatccg cgccgaaacg gtggaacagc caggggaaga ccatcgcgac gagcgttgcg
     6001 atcgagcagc tggcgaacag cgcgaggccg accgccaccg caacgtggcc gtcgtcgaac
     6061 accagcagcg cgaacggcag gaacgcggcc gccatcagga ggcccacaac gcctccggtg
     6121 gccagctccc gccagacgac cttccgcagc tccaccccga ccgagagccc gcggatgagg
     6181 acggtctcgg tctgcgtgcc gacggcgtcg gccatgtaga cgaccgccgg gaggaagaac
     6241 gccagcagca cggtcttctc gagctgctcc tcgaagccac ccacgatgac cgcggaggcc
     6301 atcgctccgg ccaggcccac cagcagccac gggatgcggt gccacaggcg gtcggcgacc
     6361 ccctcctgcg cggctccgcg cgcctcgacg gtgccggcca ggtagccgcc gagccgggcc
     6421 aggtcctcgt cgtgctcgtg taggagcacc cccaggaggc ggctgggcgg aatcagcccg
     6481 cggaactcgc cgaagcggcc ctcgaccgca accgtcgcct ccccacgcat cacggtctcc
     6541 cacacgaccg cctcctcgtc ggtggccgac ccgatggtcg gcggcgcccg gtccatgagg
     6601 tcggccatga gcgcgtcgcc gggcgcgccc aggacatcct cgatcgtggc cacgccgacc
     6661 aggcgccggc cgtcgagaac ggcgaccgcg cccgcgtagt cgaagctcgc gccggacacc
     6721 tccgcccgga catcggccgc tgtccggttc ggccgcgagc gtggcacgcg cccggtggcc
     6781 agctcgcggg cgatgcccag ccggggcgcc gccgccgcta ctcgccccac gggctgtctt
     6841 cgaaggggcc gccgaggccc ggcaactcct gcacgccggc cgcgggctgg atcgactcgg
     6901 tcagccccgc ggccgcgtcc tgggccgcca ggtagggggc caggtagttc gcggcgatct
     6961 tggcctgcgg ccaccacagc ggggacgtgg cagcggtcga agccggggcg ccggggccac
     7021 ggtctgaccg taggaagcga gccgtgcccc cggtcagcag cttgcccttc agggtcggcc
     7081 ggaaggggcg cgggtcgatg tccaccccgg cttcggcggc gatcatctcc gcggccgcat
     7141 cggcctgagc ggcggcgatc ccgccctgct tgatgccgaa gttcgtcccg tcgcccgcgg
     7201 cgtacaggcc ctgtgccccc acgacccggg cgtggcggtc gatggggatg aagccgtcgc
     7261 ggtcgtgcgg caccccgggt accgcggggc cggccaggcg cggcagggcg atcacgcgct
     7321 cgtaggcgat cggcgtctcc tcgaacggga ccaccagctc ccccgccggg gacccctcgg
     7381 cggcgaccga ccgcgccagc cgcacgcccg cctggtcgag cacgccggcc acctcctcgg
     7441 cggcctcgcg cccgaacacc gcgagcggct gtcgttccgg ggtgtacacg accagctccg
     7501 gctcctgacc tgcgtcacgg gcccgcttcg cggtcatgag cgccagctcg taggccggga
     7561 gcgaccaagc cacacccgac ggcacgacga aggcgacgct ctggaccctg ccctgctcga
     7621 cggcggtgac caccgccctg accgcctggg cgtcggccga gccggcgaac gtgaccgcgt
     7681 ggcgccagac gggctcaggc gtggcgccga gggccagcag gagcgcgtcg tacgcgatcg
     7741 tcgcgccgct cgtcgtgtgc aggcagcgag cccggccgtc cacgcgctcg agtgcgtcct
     7801 ccacgagctc ggcctggaac gcctcggcga ttgcggccag ggcgtagcga cgcggccggc
     7861 cctgcccgaa gggctcggcc accgacgccg gccggtagac gaagtccggg ccgggcgcga
     7921 cgagcgtgat ccggacccgt tcgccggcga gcgcgcggag accgatcagc gcctcgaggc
     7981 cggcgacacc ggcccccgcg atggcgacct cggtcatggg cccacgtggg tcgtgtgggc
     8041 gatggcggag cacctgcgcg cgaaggcctc gacctgatcc cagtcggtgt attcgtagtc
     8101 ccggctggtg tcggtcgggt ggtggccgcg cttcatgagc aggcgcatca gcagcctggt
     8161 catgaagtcg tactccaggt actgcagggc gccggcgatg acggacgtct cggtcggcgt
     8221 ccagccggtc tgctcggcga agtcgtccac gtacttccgg ttctcccggc gggactcgtc
     8281 ggcgtcctcc gcggcgctca gggacaccga gaagaacgcc gacggcatgc cgttcagcgt
     8341 cggcgcgtgg gccttggccc agtcccgtag gtcgcgctgg tggtgaccgg cgtggaccga
     8401 cgccccgacc acgacggcgt cgaagccgga cggatctgtc gccacggtgc cgtcggcctt
     8461 ctcggtcacg acgtccgccc cctccccgcg caggacatcg ccgatccggg tggcgatctt
     8521 ctcggtctgg ccgtgggtgc tggcgtacag gacgaggagc ttcggcatgg cggcctccgg
     8581 ttcggggaag gtccggccag tctggtcggg acggccacgt gtggcatccg tggaaagccg
     8641 cgcccggggt gcgggacatc cgccggtcgc acggaccgcg tggatgcgtt agaccacgct
     8701 catggcgacc acgtccgagc gggtcgcgcg cggcaaggcc gcccgcgccg aggcaccgcg
     8761 ctccagccac gcggagttcg accccagcgg tcgccctgat ccggtcgcgc tcctcgagca
     8821 acaggccgcg acgcgcgtcc ccgagctggt gcccatccga cacggccgca tgctggcgtc
     8881 gccgttcacg ttcttccgcg gggcggcgaa gatcatggcc cacgacctgg ccggcacgcc
     8941 gcggtcgggc ctggacgtgc agtgctgcgg cgacgcgcac ctgtccaact tcggggtgtt
     9001 cgcctcgccc gagcgcaagc tcgtgttcga cctgaacgac ttcgacgaga cgctgccggg
     9061 gccgtgggag tgggacgtca agcggctggc ggcgagcgtg ctggtcgccg cgggcgagaa
     9121 cggcttcaag cgcaagcgcc aggatcggat cgtgctggag acggtgcgca gctaccgaac
     9181 cgcgatggcc gagttcgccg ggatggggaa cctggacgtc tggtactcac acgccgagat
     9241 cgagaacgtg ctggccgagt acgccccgca gctggcggcc agggccgtca agcgcacgcg
     9301 gaagagcctg gcgaaggcgc gtacgaagga cagcacggcg gcgttcgcca agctcaccga
     9361 ggtggtcgac ggccaggtgc ggatagccga cggctcgccg ctgatcgtcc cggtcgacaa
     9421 gctcgcccgc gatacggggc gggacgagac gctggaccgg ctccgcgagc tcatggacga
     9481 ctaccgcgac agcctggacc ccgaccggcg cgcgctgctg gcgtccttcg agctggcgga
     9541 cgtcgcgcgc aaggtggtcg gggtcggcag cgtgggcacg cgcgcgtgga tcgcgctgct
     9601 gttcggccgc gaccagcagg acccgctgtt cctccagctc aaagaggccg aggcctcggt
     9661 gctcgaggag ttcctcgcgc cgagcgagta ctccaaccac ggccagcggg tggtggccgg
     9721 acagcgcctg atgcaggcca ccagcgacat cttcctggga tggctgcaca gcgaggtggg
     9781 catggacggg cgcccgcgcg acttctacgg ccgtcagctc aaggactgga aggggtcggc
     9841 ggagatcgag cggatggacg ccgaggggat ggccgtgtac gggcggctct gcggctgggc
     9901 cctggcccgc gcccacgccc gcagcgggga ccggatcgcc atcgccgcct acctgggcgg
     9961 cagcgacacg ttcgaccgcg cggtgctcgc gttctcgcgc gactacgccg agcagaacga
    10021 ggccgaccac cgcgcgctgg gcgccgcggt cgacagcggc agggtcgcgg cgcagcccgg
    10081 tctctgacgg gctactcgac gagcttccag tacgagtcct tgcggctgat caagccgtcg
    10141 cggaactcga gcaggtcaca tccgcggacg tggacggcac ggccgtcggt gtccgtgccg
    10201 gtgaccgtcc actcggaggc cccgcggtcg ccggcgaccc agtggcggtc gtccccgtag
    10261 tggatgtcgg ggatcccggc cagacgggcc tcgatcccct ggcgcacctg ctccttgccc
    10321 accaggtgcc ggccgccggg cgccgggccg cgcggcatgt ccagcacgca gtcctcgtgg
    10381 aagaactcca tcacggcgtc ggcgtcgtgg cggttgaacg ccgcgaggaa cgcctcgagc
    10441 acggccacgt cgacggtggg ctcggtcacg atcccagcgt cccacaccgt caccgctccc
    10501 gccgggtatc gacacccgtg tcgacaggcc agcaggcacc attgccccgg ccgggtggac
    10561 ttgccatgtt gacttcaggc gcggccgcgc tcggccgcga atgccactca taggaggtct
    10621 gcaatgagcg ttcgtacgac gggcgaggca cccgcgcccg gccgatcgac cggtgccggc
    10681 ccgccagcgg tctccggcga cgacgccggc tacggctggg tcgccttcgc gggggtcctg
    10741 ttggtcatcg cgggcatgct gaacgccatc tacggcatag cggccatcga tgacgcgcag
    10801 ttcttcgtgg gcaacacgga gttcgtgttc tccaacctca atacatgggg ctggatcgtg
    10861 ctgtgcctcg gggtcgccca gctgctgatc gccttcgggg tcttcgcgaa gaaccagttc
    10921 gcgcgctgga ccggcgtgtt cgtgctgggc atgaactcga tcgcgcagat gctgttcatc
    10981 gacgcgtatc cgttcctgtc gctgtcgatc ctggcgatga acctgctggc catctacggg
    11041 ctggccgcgt acggcggccg gatcgccacc acccgctaga cacggggaca ttccgggcgc
    11101 gccgcgtgtc ggcggcgcgt ccggcctagt acccgagcgg gtgctcggtc tcgggcagct
    11161 cgtccagcga ccggcgcatg tcggagaccg cccgcagggc gcgcatcgcc ggcgggagct
    11221 cccacgcggc ggccagcggg aacccgatgc acaccgcgac caggaacggc agcgcggcgg
    11281 cgggcgccgg cgccagcgtg gccgcgatca gcaggagcgt gctggccagc gccgcggtgg
    11341 cggccagggc acaggtcttc agggcctggc ttcggttggg cagtggacga gggagcatgg
    11401 ccggtggggc gccgaacgcc ggcgccagtg ttcgtgtccc cccgcacacc ggcggcgaaa
    11461 catgcgctag gcggagctgt cccacaacgc cgcggtctgg cgctcggcct cgtcgtcgtg
    11521 cgtgcgctgg aagttgcggc cgccccgggc gaaggtctgt gcgacggccg ccgggacctc
    11581 acggtcgccg aacggccgct cgagccagcg cgtgggccgg acgtggtgga tgcccacctc
    11641 gcgcgcggcg ggcgagtcgt cgtagcgcca cggtccggtg atcctgccca ggtggtagct
    11701 cccgtccggt ccccgggtcc agacgaacgt gccctcgggc agggatgcga accggtcgag
    11761 catgcgggcg gccttctcgc cctggccgtc tccgatgcct acgacaccgt gctcgagccc
    11821 gaaggcggcg ccggcaccct ccggggcgtc gcggtcacga gcccgcatcg gcgcccggta
    11881 g
//
LOCUS       DASUPW010000534         2953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_2062555, whole genome shotgun sequence.
ACCESSION   DASUPW010000534 DASUPW010000000
VERSION     DASUPW010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2062555"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="VFY44_12045"
     CDS             complement(<1..1096)
                     /locus_tag="VFY44_12045"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5911216.1"
                     /translation="MAPTDSDLERSSPKEHSRRLGSARDAMLAVTRLLSPDGDLRAPD
                     AVRESLVNEAREFFGVRRVVLLRTEGQERLRILASSPAARIPRGTIGVDEFPVLRDPA
                     ENDAVAFASGEAALALDTALGSMDPARSLLALPMQIEARRGHILILADAEGRTFADEE
                     VQVAQAFAAATSATLAQLRMAGERAVQIAQQAALARAAKTLNDSLDLNRVLVRISHEA
                     ASILDADNATVYRGDPEEGMVVEATFGLPPEVIGYRMPTGHGLAGKVAQQDRSLLTND
                     YQGMPDPGDFPLFRETRSALAVPMHWDGELRGVLTVAYRREHVVTNDQLKLLEAFGEL
                     AAAACRNASAHAGLVLAARTDGLTGCLNHAA"
     gene            complement(1128..2270)
                     /locus_tag="VFY44_12050"
     CDS             complement(1128..2270)
                     /locus_tag="VFY44_12050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5911217.1"
                     /translation="MEAGTAPSASGLTDEQRDFVDAIRDFAGREKFDIPEDDHHSDEV
                     AAKMAELGWYGLQIDEQYGGSGGSFLDACLFLEEVARGQIPIGGYGVTLIVVGALNKF
                     GSEEQKAEHLGHVSKGGGTLAIAMSEPDSGSDVASLKCKARLEDGEWVLSGSKMWCSY
                     AHKASHTLIVCRTEEGSERHEGLSMIFVPHDADGFTITPIDTMGGRETNELHLDDVRV
                     PESALLGTAGNGWIQLMSGLNYERTILAASAVGLAQRAFDDTLEYVKERKQFGRPVGS
                     FQVLQARLADLATELIQARLLVRHVASLTDEDPTRMLPQEASMAKLAATELAKKVALE
                     AVQMGGGYGYATEYPMERHLRTAVVTTIYGGTSEIQRNIIAKTLGL"
     gene            complement(2280..>2953)
                     /locus_tag="VFY44_12055"
     CDS             complement(2280..>2953)
                     /locus_tag="VFY44_12055"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5911218.1"
                     /translation="ITDPDAAPHKRASMILVPIDTPGFTGVRPVPVMGHAAGPGHWEV
                     LYEDVRVPEANLLGPRGEGFSIAQARLGPGRIHHCMRAIGSAERAFELMCERAHQREA
                     FGGKLADKQFVQDFVAKSRMEIDAARWMVLHAAWKMDTEGKRAARQEISMTKVVAAQM
                     HQDVLDRAMQVHGALGMSDDTPLAGLWRQGRWLRIADGPDEVHKMVIAMRELNKHKPR
                     EAAAV"
BASE COUNT          366 a         1072 c         1052 g          463 t
ORIGIN      
        1 tggcggcgtg gttgaggcag ccggtcagcc cgtcggtgcg ggcggccagc accaggccgg
       61 cgtgggcgct ggcgttgcgg caggcggcgg cggccagctc gccgaaggcc tcgagcagct
      121 tgagctggtc gttggtgacc acgtgctcgc ggcggtaggc caccgtcagg acgccgcgca
      181 gctctccgtc ccagtgcatc ggcacggcca gcgcgctccg ggtctcgcgg aacagcggga
      241 agtcgcccgg gtcgggcatg ccctggtagt cgttggtcaa cagcgaccgg tcctgctggg
      301 ccaccttgcc ggccaggccg tggccggtgg gcatccggta gccgatcacc tcggggggaa
      361 ggccgaaggt ggcctccacc accatcccct cctccgggtc gccgcggtag acggtggcgt
      421 tgtcggcgtc caggatgctg gccgcctcgt ggctgatccg caccagcacg cggttcaggt
      481 ccaggctgtc gttcagcgtc ttggccgcgc gcgccagggc ggcctgctgg gcgatctgca
      541 cggcgcgctc gccggccatg cggagctggg ccagcgtggc cgaggtggcg gcggcgaacg
      601 cctgggccac ctggacctcc tcgtcggcga aggtgcggcc ctcggcgtcg gccaggatca
      661 ggatgtgccc gcggcgggcc tcgatctgca tcggcagcgc cagcaggctc cgggccgggt
      721 ccatcgaccc cagcgccgtg tccagcgcca gcgccgcctc gccggaggcg aaggcgacgg
      781 cgtcgttctc ggccgggtcg cgcagcacgg ggaactcgtc caccccgatc gtcccccggg
      841 ggatccgggc ggccgggctg gaggccagga tgcgcagccg ctcctggccc tcggtgcgca
      901 gcagcaccac ccggcggacg ccgaagaact cgcgcgcctc gttgaccagg ctctcgcgca
      961 ccgcgtcggg ggcccggagg tccccgtcgg gcgaaagcag ccgcgtgacg gccagcatcg
     1021 cgtcccgggc gctgcccagg cggcgcgaat gctccttggg tgagctccgc tcgagatcag
     1081 agtccgtcgg cgccatcccc ctaggtatcg gccgccacgg ctggccccta cagccccagc
     1141 gtcttggcga tgatgttgcg ctggatctcg gacgtgccgc cgtagatcgt ggtcaccacc
     1201 gccgttcgca ggtgccgttc catcgggtac tcggtcgcgt agccgtagcc gccgcccatc
     1261 tggaccgcct ccagcgccac cttcttggcc agctcggtgg ccgccagctt ggccatcgag
     1321 gcctcctgcg ggagcatccg cgtggggtcc tcgtcggtca ggctcgccac gtggcgcacc
     1381 agcaggcggg cctggatgag ctcggtggcc aggtccgcca gccgtgcctg cagcacctgg
     1441 aagctgccca ccgggcggcc aaactgcttg cgctccttga cgtactccag cgtgtcgtcg
     1501 aacgcgcgct gggccaggcc caccgccgag gcggccagga tcgtgcgctc gtagttcagg
     1561 ccggacatga gctggatcca gccgttgccc gcggtgccca gcagggcgct ctcggggacg
     1621 cgcacgtcgt ccaggtgcag ctcgttggtc tcgcgcccgc ccatcgtgtc gatcggcgtg
     1681 atcgtgaacc cgtccgcgtc gtggggcacg aagatcatcg acagcccctc gtggcgctcg
     1741 gagccctcct cggtgcggca cacgatcagg gtgtggctgg ccttgtgggc gtaggagcac
     1801 cacatcttgg agccggagag cacccactcg ccgtcctcca ggcgggcctt gcacttcagc
     1861 gacgccacgt cggatccgga gtcgggctcg gacatggcga tggccagcgt gccgccgccc
     1921 ttgctcacgt ggcccaggtg ctcggccttc tgctcctcgg agccgaactt gttcagcgcc
     1981 ccgaccacga tcagcgtcac gccgtagccg ccgatcggga tctggccgcg cgccacctcc
     2041 tcgaggaaca ggcaggcgtc caggaagctg ccgcccgagc cgccgtactg ctcgtcgatc
     2101 tgcagcccgt accagcccag ctcggccatc ttggccgcga cctcgtccga gtggtggtcg
     2161 tcctcgggga tgtcgaactt ctcacgcccg gcgaagtcgc ggatggcgtc cacgaagtcg
     2221 cgctgctcgt cggtcagccc tgaggccgac ggtgccgttc ccgcttccat gtgactcctc
     2281 tagaccgccg cggcttcgcg cggcttgtgc ttgttcagtt cgcgcatggc gatgaccatc
     2341 ttgtgcacct cgtcggggcc gtcggcgatg cgcagccagc gcccctggcg ccacaggccg
     2401 gccagcggcg tgtcgtccga catgcccagc gcgccgtgga cctgcatggc gcggtccagc
     2461 acgtcctggt gcatctgggc ggccacgacc ttggtcatcg agatctcctg gcgcgcggcg
     2521 cgcttgccct cggtgtccat cttccaggcg gcgtgcagca ccatccagcg ggccgcgtcg
     2581 atctccatcc gcgacttggc cacgaagtcc tggacgaact gcttgtcggc cagcttgccg
     2641 ccgaacgcct cgcgctggtg ggcgcgctcg cacatcagct cgaacgcccg ctcggccgag
     2701 ccgatggcgc gcatgcagtg gtggatgcgg ccggggccca ggcgggcctg ggcgatgctg
     2761 aacccctcgc cgcgcgggcc cagcaggttc gcctcgggta cccggacgtc ctcgtacagg
     2821 acctcccagt ggcccgggcc cgcggcgtgg cccatgaccg gcaccgggcg cacgccggtg
     2881 aagccggggg tgtcgatggg caccaggatc atgctcgcgc gcttgtgcgg cgcggcgtcg
     2941 ggatcggtga tga
//
LOCUS       DASUPW010000535         3249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_2062722, whole genome shotgun sequence.
ACCESSION   DASUPW010000535 DASUPW010000000
VERSION     DASUPW010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2062722"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VFY44_12060"
     CDS             complement(<1..385)
                     /locus_tag="VFY44_12060"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX5911219.1"
                     /translation="MEDPKTVRQEAHHGVVTTAVQEPPTVAALRAGDEVDAVFACTRK
                     DRLTGRSGSAYLALELRDRTGAIPARVFRDPDFAAARFERGDLVRVQGRVDRFRDELQ
                     LEVRAIERAEGHGLDPATFLPMAYRD"
     gene            399..1274
                     /locus_tag="VFY44_12065"
     CDS             399..1274
                     /locus_tag="VFY44_12065"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX5911220.1"
                     /translation="MSDLRWTARPTLRRPVLVAAFQGWNDAGDAATAAAEFIEARFDH
                     VEVACIDPDEFLDFTSVRPDVKLIDGHMREIEWPETRITAAEVPGAERDLMVLSGVEP
                     SLRWRRYCDAVIEAAHAMGASMVITLGSLLADVPHSRAVSISGIGSDAAAIDRLGFEH
                     TSYEGPTGIVGVLQHACQAAGLDSLSLWASVPHYVAATPNPKVALALVRAFEGATGTI
                     IDAGELEEAAEDYERQVSAAVASDPEVKAFVERLESAMDEVTEDSDPPENLPSADAIA
                     SDFQKFLRHKGPDGP"
     gene            complement(1280..>3249)
                     /locus_tag="VFY44_12070"
     CDS             complement(1280..>3249)
                     /locus_tag="VFY44_12070"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX5911221.1"
                     /translation="ALPFRLHGSAAYFQRAEVRDLLAWLRLLANPADSGAAVRVMARP
                     PVGLHSVDIARLTQLARRRKVDMPSAVSAALEGPQLTPEGRDRAQYFLRLYRPACAAF
                     EDRRPDAFVLRLIERVGIRRQQVFATQADTVERLRNIAVMPELATAFMRREPGASPRD
                     FARYLRAVADSGLEWEEVPAPEHEGAVQVLDIDAARGREFDHVFVAGLTAGALADRER
                     AGAGVPGELAKHRDGLPDARERAGRVVALAASRARESLVLSWSESGEARPAAPVEDAR
                     AALSAPEEHMEEELFGPAEGLHSTFRIMRDELLDTVAQVGGQLGEMRLDAAADVDRAV
                     ARFLELVKVAALIDRSAAGQEVEQALSEVNALLSQGASAPQREVLESSPLDDWLRDAG
                     RDASEIPTSGAATTEPSLDPFIPRRGDGVMLSASDIETYRLCPLKYKFARVFRIPQES
                     TINQRFGIVLHQVLERFHKSPSPSRDELFELFEQSWRRGGFGDSDDELQFRERALDAL
                     EMYWVRTRDEEGEPEWFERSFSFQLGPHLLRGRVDRVDRLPDGSYELIDYKTGKAKTE
                     DDLRQDIQLSVYQMGARESWGVRTSAQSYMYVMTGEKVPVAHTEEELDRVKATVERIG
                     EGILRQEFQPTPSQEICSFCDYRIICPAAER"
BASE COUNT          444 a         1214 c         1175 g          416 t
ORIGIN      
        1 ggtcgcggta ggccatcggc aggaaggtgg ccgggtcgag cccgtggccc tcggcgcgct
       61 cgatcgcgcg gacctcgagc tggagctcgt cgcggaagcg gtcgacgcgg ccctggacgc
      121 gcaccaggtc gccgcgctcg aaccgcgccg cggcgaagtc ggggtcgcgg aacacccgcg
      181 cggggatggc gccggtgcgg tcccgcagct cgagcgccag gtaggccgac cccgatcgcc
      241 cggtcaggcg gtccttgcgc gtgcaggcga acacggcgtc gacctcgtcg ccggcgcgca
      301 gcgcagcgac ggtcgggggc tcctggacgg cggtggtcac caccccatgg tgggcctcct
      361 ggcggacggt cttcgggtcc tccactaccg tgaacgtgat gtccgatctg agatggaccg
      421 cgcggcccac cctgcgcagg ccggtcctgg tggccgcctt ccagggatgg aacgacgccg
      481 gtgacgccgc gaccgccgcg gccgagttca tcgaggcccg gttcgaccac gtcgaggtgg
      541 cgtgcatcga cccggacgag ttcctggact tcaccagcgt ccggcccgac gtcaagctca
      601 tcgacggcca catgcgcgag atcgagtggc ccgagacccg gatcaccgcc gccgaggtcc
      661 ccggcgccga gcgcgacctg atggtgctca gcggcgtcga gccctcgctg cgctggcgcc
      721 gctactgcga cgcggtcatc gaggccgccc acgcgatggg ggccagcatg gtcatcacgc
      781 tgggctcgct gctggccgac gtgccccact cgcgcgccgt gtcgatcagc gggatcggct
      841 ccgacgccgc ggccatcgac cggctgggct tcgagcacac cagctacgag ggccccaccg
      901 gcatcgtcgg ggtgctccag cacgcgtgcc aggcggcggg cctggactcg ctcagcctgt
      961 gggcctcggt cccgcactac gtggcggcga ccccgaaccc gaaggtcgcg ctggcgctgg
     1021 tccgcgcctt cgagggcgcc accggcacca tcatcgacgc cggtgagctg gaggaggccg
     1081 ccgaggacta cgagcgccag gtcagcgccg ccgtggccag cgaccccgag gtcaaggcct
     1141 tcgtcgagcg gctcgagagc gccatggacg aggtcaccga ggacagcgac ccgcccgaga
     1201 acctgccctc ggccgacgcc atcgccagcg acttccagaa gttcctgcgc cacaagggcc
     1261 cggacgggcc ctagcccgcc taccgctcgg ccgccgggca gatgatccgg tagtcgcaga
     1321 agctgcagat ctcctgtgac ggggtgggct ggaactcctg gcgcaggatg ccctcgccga
     1381 tccgctcgac cgtcgccttg acccggtcca gctcctcctc ggtgtgggcc accggcacct
     1441 tctcgcccgt catcacgtac atgtagctct gcgccgaggt gcgcacgccc caggactcgc
     1501 gcgcgcccat ctggtagacg gacagctgga tgtcctggcg caggtcgtcc tcggtcttgg
     1561 ccttgccggt cttgtagtcg atcagctcgt aggagccgtc gggaaggcgg tccacgcggt
     1621 ccacgcggcc gcgcagcaaa tgcgggccga gctggaacga gaagctgcgc tcgaaccact
     1681 cgggctcgcc ctcctcgtcg cgcgtgcgca cccagtacat ctccagcgcg tccagcgcgc
     1741 gctcgcggaa ctgcagctcg tcgtcggagt cgccgaagcc gccccggcgc caggactgct
     1801 cgaacagctc gaacagctcg tcgcgcgagg gcgagggcga cttgtggaag cgctcgagca
     1861 cctggtgcag cacgatcccg aagcgctggt tgatcgtcga ctcctgcggg atccggaaga
     1921 cgcgcgcgaa cttgtacttc agcgggcaca ggcggtacgt ctcgatgtcc gacgccgaca
     1981 gcatcacgcc gtcgccgcgg cgggggatga aggggtccag cgacggctcg gtggtggcgg
     2041 cgccggaggt ggggatctcc gacgcgtccc ggcccgcgtc ccggagccag tcgtccagtg
     2101 ggctggactc cagcacctcg cgctggggtg cgctggcgcc ctggctgagc agcgcgttga
     2161 cctccgacag cgcctgctcg acctcctgcc ccgcggcgga ccggtcgatc agcgcggcca
     2221 ccttgaccag ctccaggaag cgggccacgg cgcggtccac gtcggcggcg gcgtccaggc
     2281 gcatctcgcc aagctgcccg cccacctggg ccacggtgtc cagcagctcg tcgcgcatga
     2341 tccggaacgt ggagtgcagc ccctcggcgg ggccgaacag ctcctcctcc atgtgctcct
     2401 cgggagccga gagcgcggcg cgcgcgtcct ccaccggcgc cgcgggccgg gcctcgcccg
     2461 actcggacca ggacagcacc aggctctcgc gcgcgcggga cgcggccagg gcgaccacgc
     2521 ggccggcgcg ctcccgggcg tccggaagcc cgtcgcggtg cttggccagc tcgcccggca
     2581 cgccggcgcc ggcccgctcc cggtcggcca gcgcgccggc cgtcagcccg gcgacgaaca
     2641 cgtggtcgaa ctcgcgcccg cgggcggcgt cgatgtccag aacctggacg gcaccctcgt
     2701 gctcgggggc gggcacctcc tcccactcca ggcccgagtc ggcgaccgcg cgcaggtagc
     2761 gcgcgaagtc ccgcgggctg gcacccggct cgcggcgcat gaacgcggtg gccagctcgg
     2821 gcatcacggc gatgttgcga aggcgctcga cggtgtcggc ctgggtggcg aacacctgct
     2881 ggcggcggat gcccacgcgc tcgatcaggc gcagcacgaa cgcgtccggg cggcggtcct
     2941 cgaacgcggc gcacgcgggc cggtacaggc gcaggaagta ctgcgcgcgg tcgcggccct
     3001 ccggcgtcag ctgcggcccc tccagcgccg ccgagaccgc cgacggcatg tccacctttc
     3061 gccgccgggc cagctgggtc aggcgcgcga tgtccaccga gtgcagcccc accggcggcc
     3121 gcgccatcac gcgcaccgcg gcgcccgagt cggccgggtt ggccagcagc cggagccagg
     3181 ccaggaggtc gcgcacctcg gcgcgctgga agtacgcggc cgagccgtgc aggcggaagg
     3241 gcagcgccc
//
LOCUS       DASUPW010000536         4160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1507425, whole genome shotgun sequence.
ACCESSION   DASUPW010000536 DASUPW010000000
VERSION     DASUPW010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507425"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..433
                     /locus_tag="VFY44_12075"
     CDS             68..433
                     /locus_tag="VFY44_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911222.1"
                     /translation="MLGLSACGGDDGGSDDKGSGDSAASTPSKAASPSHAAVLKCLQD
                     AGLEAEDQSNSSGKKVGIDYPAGRLVISFEKSAEDAETYASVAKTNGEGTIVKGTIVA
                     TIPVDAEAQSAEDTVEGCI"
     gene            527..1261
                     /locus_tag="VFY44_12080"
     CDS             527..1261
                     /locus_tag="VFY44_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911223.1"
                     /translation="MRNRLSRPSPALVVASISLFIALGGTGYAAATGSIDGREIKNST
                     IQGKDVKNSSLTGSDVKTSGLTGSDVKSDSLTGGDIAESSLGKVGTAGRADSATSATS
                     ATNAATASNATALGGASPDSYRTVTAQDTRTDNLNLGASDADVLTTQITLPVAKQVMA
                     TAVVDLFSDGGDNDNANCNVQMATTEGVRVSQQIMDSGIADVANMGLTQSQALPAGTH
                     TVTVECAEGNVSNTTVDEAALTVWTN"
     gene            complement(1323..1394)
                     /locus_tag="VFY44_12085"
     tRNA            complement(1323..1394)
                     /locus_tag="VFY44_12085"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1360..1362),aa:Asn,seq:gtt)
     gene            complement(1429..2418)
                     /locus_tag="VFY44_12090"
     CDS             complement(1429..2418)
                     /locus_tag="VFY44_12090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5911224.1"
                     /translation="MTEDSLPDDSELTDVAAEEADEAESDGGPDAYQRFDEGGVVSAG
                     VPAAPPEGGTEDAVRLYLHNIGRVALLTRQDEVRLAKRVEANDMQAKNDLIEANLRLV
                     VSVAKRYQGRGLTLLDLIQEGNMGLIRAVEKFDWRRGFKFSTYATWWIRQSMTRALAD
                     QSRTIRIPVHMVERMNKVARARRELSQKLERDPTPEEISELVEMPPDKVEELLKLGQE
                     PVSLEAPIGSGDDPAQLADFIEDEVETRPDAQVAKLIREADVQEVLDSLPERERRVIE
                     LRFGLDPEGPMTLEDIGKVVGLTRERVRQIEVKTLQMLKASGRAARLEGMADH"
     gene            complement(2415..>4160)
                     /gene="dnaG"
                     /locus_tag="VFY44_12095"
     CDS             complement(2415..>4160)
                     /gene="dnaG"
                     /locus_tag="VFY44_12095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX5911225.1"
                     /translation="TQDSIERLRETADIVTLISRKTDLRRVGSRWTGLCPFHEERTPS
                     FSVDPERGLYHCFGCGVGGDAIRFVMETDQLDFPGAVEQLAEDNHVELKREREDPEEE
                     QRRERRERLLGLVERATAFYATVLRDSAEAGRARDYLRERGLSDQVLSDFRVGYAPKA
                     WDRLLTGARRQGYTEDELLAAGLSTRGKNGGVYDRFRERITFPLADTRGRVLGFGARA
                     MRDDQGAKYINTAEGELYHKGRQLFGIDRARAAVAKAQRVIVVEGYTDVLALHDAGMP
                     ETVGVMGTALTKDQMGELARLVGTEGTIYLALDADRSGQEAMLRAARLAEDRAVSLRV
                     VRLPDGQDPADLVTGQGREVMTDLVTKALSVLEFAVGRVLADGELDDPEGRDRALNAA
                     RELIAAAPARSARRDHLVRMVADRLDVPADYVEASAAGQIAPAAAGPAAAPAAAPAKT
                     DPEVSFLALCLAAGADGREQLQQLTDQHLTSATVRQARDHLYEHFDDPLSALSSDQPQ
                     VAMVVSGAARHAEDLRPASATKLRRDFLRIDLRRIERQMRAARQDEDFARLKELSSEH
                     QRAKTAMGAAIGSAP"
BASE COUNT          612 a         1450 c         1494 g          604 t
ORIGIN      
        1 cgttctgtcg gccaggggaa ggaatcggat gggatctcgg gtccttgtag gaatgtgcgc
       61 gggcgtcgtg ctcggactgt cggcctgcgg cggggacgac ggagggagcg acgacaaggg
      121 gtcgggcgac tccgcggcct cgaccccgtc caaggccgcg tcgccgtccc acgccgcggt
      181 cctgaagtgc ctccaggacg cgggcctcga ggccgaggac cagagcaact cgtcgggcaa
      241 gaaggtcggg atcgactacc cggccggccg cctggtgatc tcgttcgaga agtccgccga
      301 ggacgccgag acctacgcca gcgtcgccaa gaccaacggc gagggaacga tcgtcaaggg
      361 gacgatcgtg gccacgatcc cggtcgacgc cgaggcccag tcggccgagg acacggtcga
      421 gggctgcatc tgagctgaga ccaggtcgag accagggcga tactgcgccg gggctcggct
      481 cgccacagga tcggggtgta cggaaccccc gaaccaagga cgcaaaatgc gcaatcgcct
      541 gtcccggccc tcacctgcgc tggtggtggc ttcgatctcc ctgttcatcg cccttggtgg
      601 taccggctac gccgcggcca ccggctcgat cgacggacgt gagatcaaga acagcaccat
      661 ccagggcaag gacgtcaaga actcgtccct gaccggaagc gacgtcaaga ccagcggtct
      721 gaccggcagc gacgtcaaga gcgactcgct caccggcggt gacatcgcgg agtcgagcct
      781 cggcaaggtc ggcaccgcgg gccgcgctga cagcgcgacg agcgcgacga gcgcgaccaa
      841 cgcggcgacg gcgtcgaatg ccaccgctct gggcggagcg agccccgaca gctaccgcac
      901 cgtgaccgcg caggacaccc ggacggacaa cctgaacctg ggcgcgagtg acgcggatgt
      961 gcttaccacg cagatcaccc tgccggtcgc gaagcaggtc atggccacgg cggtggtcga
     1021 ccttttcagc gacggtggtg acaacgacaa cgccaactgc aacgtccaga tggcgacgac
     1081 cgagggtgtg agggtcagtc agcagatcat ggacagcggg atcgccgatg tggcgaacat
     1141 gggcctgacc cagagccagg cgttgccggc cggcacccac acggtgacgg tggagtgcgc
     1201 cgaaggaaac gtctccaaca ccacggtgga cgaggcagcc ctgacggtct ggacgaactg
     1261 acggggacgc agctgaggcg gcggccgcga cgtgcggccg tcgcccagcc ccgcgttccg
     1321 agctccgggg gtgggattcg aacccacaac ccttcgatta acagtcgaat gctctgccgt
     1381 tgagctaccc cggaacgaag gggtcacgat accgggccgg caaggcccct agtggtcggc
     1441 catgccctcg agccgcgcgg cgcggccgga ggccttgagc atctggagcg tcttgacctc
     1501 gatctgccgc acgcgctcgc gcgtgaggcc gaccaccttg ccgatgtcct ccagggtcat
     1561 cgggccctcg gggtccaggc cgaagcgaag ctcgatcacg cgccgctcgc gctcgggcag
     1621 gctgtccagc acctcctgca cgtcggcctc gcggatgagc ttggccacct gggcgtcggg
     1681 acgggtctcg acctcgtcct cgatgaagtc ggccagctgg gccgggtcgt cgcccgagcc
     1741 gatcggcgcc tccagcgaca ccggctcctg gcccagcttg agcagctcct cgaccttgtc
     1801 gggcggcatc tcgaccagct cgctgatctc ctcgggcgtg gggtcgcgct ccagcttctg
     1861 ggacagctcg cggcgggccc gggccacctt gttcatccgc tcgaccatgt gcacggggat
     1921 gcggatggtg cgcgactggt cggccagcgc gcgggtcatg gactggcgga tccaccaggt
     1981 ggcgtaggtc gagaacttga accctcggcg ccagtcgaac ttctcgaccg cgcggatcag
     2041 gcccatgttg ccctcctgga tcaggtccag aagggtcagg ccgcggccct ggtagcgctt
     2101 ggccaccgac accacgaggc gcaggttggc ctcgatcagg tcgttcttgg cctgcatgtc
     2161 gttggcctcg acgcgcttgg ccaggcggac ctcgtcctgg cgggtcagca gcgccacgcg
     2221 gccgatgttg tgcaggtaca gccggacggc gtcctcggtg ccgccctccg gcggcgcggc
     2281 gggaacgccg gcgctgacca cgccgccctc gtcgaagcgc tggtaggcgt cggggccgcc
     2341 gtcggattcg gcctcgtcgg cctcttcggc ggccacgtcg gtgagctcgg agtcgtcggg
     2401 caggctgtct tcggtcacgg tgcgctcccg atggccgcgc ccatggcggt cttggcccgc
     2461 tggtgctcgc tggagagctc cttcagccgt gcgaagtcct cgtcctggcg ggccgcgcgc
     2521 atctgccgct cgatgcgacg gaggtcgatg cggaggaagt cgcggcggag cttcgtcgcg
     2581 ctcgccggcc gcaggtcctc ggcgtgccgg gcggcgccgg agacgaccat ggccacctgg
     2641 ggctggtcgc tggagagggc ggacagcgga tcatcgaagt gctcgtacag gtggtcgcgc
     2701 gcctggcgca cggtcgccga ggtcaggtgc tggtcggtca gctgctggag ctgctcgcgg
     2761 ccgtcggccc cggcggccag gcacagcgcc aggaacgaga cctcggggtc ggtcttggcc
     2821 ggggcggcag cgggggcggc ggccggtccc gccgcggcag gcgcgatctg gccggcggcg
     2881 ctggcctcca cgtaatcggc cggcacatcg aggcggtcgg cgaccatgcg gaccaggtgg
     2941 tcgcgtcggg cgctcctcgc cggagcggct gcaatcaact cgcgggctgc gttcaaagcg
     3001 cggtctctgc cttccggatc gtccaactcg ccgtcggcga gcaccctgcc gaccgcgaac
     3061 tcaagcacag agagcgcctt cgtgaccaag tcggtcatga cttcccgccc ctggcccgtg
     3121 accaggtccg ccgggtcctg cccgtcgggc agccggacca cccgcagcga gaccgcccga
     3181 tcctcggcca ggcgggccgc gcggagcatg gcctcctgcc cagagcggtc cgcgtccagg
     3241 gccaggtaga tggtgccctc ggtgcccacc agcctggcca gctcgcccat ctggtccttg
     3301 gtcagggcgg tgcccatgac gccgacggtc tcgggcatgc cggcgtcgtg cagggccagg
     3361 acgtcggtgt agccctcgac gacgatcacg cgctgggcct tggccaccgc ggcccgggcg
     3421 cggtcgatgc cgaacagctg gcggcccttg tggtagagct cgccctcggc ggtgttgatg
     3481 tacttcgcgc cctggtcgtc gcgcatcgcg cgggcgccga agcccagcac gcggccgcgg
     3541 gtgtccgcca gcgggaaggt gatccgctcg cggaaacggt cgtacacgcc cccgttcttg
     3601 ccccgggtcg acaggccggc ggccagcagc tcatcctcgg tgtacccctg ccgacgggcg
     3661 cccgtgagca gccggtccca ggccttgggc gcgtagccca cgcggaagtc ggacagcacc
     3721 tggtcggaca gtccccgctc gcgcaggtag tcgcgcgccc gtcccgcctc ggccgagtcc
     3781 cgcagcacgg tggcgtagaa cgcggtcgcc cgctcgacca ggcccagcag ccgctcgcgg
     3841 cgctcgcggc gctgctcctc ctcgggatcc tcgcgttcgc gcttgagctc gacgtggttg
     3901 tcctcggcca gctgctcgac cgcgcccggg aagtccagct ggtcggtctc catgacgaag
     3961 cggatggcgt cgccgcccac gccgcagccg aagcagtggt agagcccgcg ctcggggtcc
     4021 accgagaacg acggcgtgcg ctcctcgtgg aaggggcaca ggccggtcca gcgcgacccc
     4081 acccggcgca ggtcggtctt gcgcgagatc agcgtcacga tgtcggcggt ctcgcgcagc
     4141 cgctcgatcg agtcctgggt
//
LOCUS       DASUPW010000537         8858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1785358, whole genome shotgun sequence.
ACCESSION   DASUPW010000537 DASUPW010000000
VERSION     DASUPW010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8858
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1785358"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VFY44_12100"
     CDS             <1..608
                     /locus_tag="VFY44_12100"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX5911226.1"
                     /translation="GFCLFASVAVAAEHALTRDGVDRVFVLDWDVHHGNGTNAAFHSR
                     PDVLFCSLHQYPFYPGTGALGDNGSGPGEGFTINLPVPAGSGEAEWLGLVEHVVVPAA
                     REFAPDLILVSAGFDAHRADPLAECNLETDSFRQLALHVASLAAELGVPAGAVLEGGY
                     DVDALAESAVATMEALEAGGTPRSVERGPLVDAAASALRPG"
     gene            complement(586..1203)
                     /locus_tag="VFY44_12105"
     CDS             complement(586..1203)
                     /locus_tag="VFY44_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911227.1"
                     /translation="MTDRTGDVHCPRCGAAAERGQLVCLECGARVALVYRRPPSWKVP
                     VAITAVVALLLGVAAFMAVAALSDDADREVAAAPPRPKPAATKTTGAKEKAEQPKKAG
                     EPAAESGALVKRGSLYQWPRTLEGFTVVINSTEDRASATSFARSASRSKPAKLGVIRA
                     DDFKTLPQGFYVVFAGFYEDRGKADQATERLNAKYPGSFTQAVER"
     gene            complement(1200..2036)
                     /locus_tag="VFY44_12110"
     CDS             complement(1200..2036)
                     /locus_tag="VFY44_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX5911228.1"
                     /translation="MRRRLREQRRVREALLLDLGALVFELHRHGRREPELLQAKASEL
                     SQVDAEVRGLSEALEADLGVVELVAAGIAGSCENCDALLSTDTRFCPACGAATKPGLE
                     AQRGQAAQVPVERQVEAQTEEHPQWEDTEHEGNGEEPEPVFAEEAQAQDHAEQETFDE
                     EPELAEAVAPEPEPEPEPEPEPEPEPEPEPEPEPEPEPEAEPEPEPEPEPEPEAEPEP
                     EPEPEPAADQPRSSSGALPAAPWLRSSDAPEGGPPPEPVKVKRKMSRLERTLRGKRKD
                     ES"
     gene            complement(2105..3115)
                     /locus_tag="VFY44_12115"
     CDS             complement(2105..3115)
                     /locus_tag="VFY44_12115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5911229.1"
                     /translation="MSRPRILYVHSRKASFVAIDREILAERYEIEDWYQPGHWGNLPR
                     LVGAVRRADLVFGWFASWHTFFPITLAWLMRKPSVQIVGGFDTANMPDIGFGYQQGGL
                     RRWASRWIMKRAGRLVTNSYSSQREIAANTPIPPEAVTVIHHGVPDPFGPLDESRPRE
                     RLALTVGPIDAGTLIQKGHLPFVQASRLLPDVRFVFVGKPLDDAIERLRAEAGPNVEF
                     TGWASDEDLTALYGSASVYVQASRHEGFGLAVTEAMLAGCVPVVVDVTAMPEVVDGAG
                     VLIASQDPAAVAAGIEEALARGPEGHRAARQRVLDEFPMEKRREGILAVTAQALGMVP
                     RT"
     gene            complement(3151..4569)
                     /locus_tag="VFY44_12120"
     CDS             complement(3151..4569)
                     /locus_tag="VFY44_12120"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5911230.1"
                     /translation="MSRRLAALLGEPEPVLAGPMWVDELYETLAAMTPSERDVVRACE
                     PGGHEDSAENVLQRGRSPWLPALLDDDDLFMAYQPIVDLSSGRTVAYEALVRGSVADD
                     ETVAGYQIVAAARAHDRIRQLDESSRRLALEQAAGALEGGERLFVNFDPMSVYDPEVC
                     LRNTWATARKVGIGIDQVCFEIVDAHRCPDIDFLRRVIESFRAEGASVALQNLGSERA
                     GVNYLRELRPDIVKLERRLTAGLEGESARRDLVGAMIAYAHELGTTVGIVGIETEGEL
                     RCARELGADIGQGFYLGPPAPRIQAADPTPVVTAKPPDPAVLGLSGDELTGLPARRAF
                     QGHVEGLLATGRSVSVLMIELSAFQRITDLLGHDVGDRVLMTVADTLRREVGEAGMVA
                     RLSGDQFLVCLDDVRSDEEAAGFGRRLADAVDAAALESELPTPHPSVGVAAAPEDGSD
                     SGTLLRQAGAALHRAGAPTSAR"
     gene            complement(4661..6544)
                     /gene="asnB"
                     /locus_tag="VFY44_12125"
     CDS             complement(4661..6544)
                     /gene="asnB"
                     /locus_tag="VFY44_12125"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX5911231.1"
                     /translation="MCGIGAMMDPAESAPSGAGQRMCEALRHRGPDGEGVVQIGPATL
                     AHTRLAIIDPAGGEQPLYSEDGQVTAIVNGEIYNHLDLRAELERAGHVFATGSDSEVV
                     VHGYEEHGPDFVRRMNGIFAFALYDQRRERLLAVRDPFGVKPLYWHSDGTRTAVASEV
                     AALIAGGLVTPELDRVALDHYLACRFVPAPRTLFAGVSKLPAASMLVVERGSAPKVSS
                     YREAPGDPLDVKGRDLEGELAERFTDAVERQMMSDVPYGAFLSGGVDSAAIVAAMARR
                     SDRPPMTFTIGFPGHGDVLDERRYARETAATLGTDHHETAMAEGDFLGELERCVRRLE
                     EPVGLPSAGALMQLSRFAAESVKVVLSGQGADEPHGGYGRHQAAAALGAAGIAGRAAG
                     PLGSLAARVAGGNERVRRASRLLGDLEPADRLVRLLEITGDDLRTELTGTSGQEAAAE
                     RRERADAVLADVGDRGLVEQALYLDTHLHLPDSLLICADKMSMSASLEQRVPFLDVEL
                     MRFVERVPARERVRIRDGKRLHRKAMGKLVPRAVVDRPKHGFSTPYEDWLRTSLGDEV
                     ARRYSPGTQLGDLIDPGTVGRLVEEHRSSRADHKSLLYCLLELSDWHQAFVQPREPVH
                     TSS"
     gene            6723..6932
                     /locus_tag="VFY44_12130"
     CDS             6723..6932
                     /locus_tag="VFY44_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911232.1"
                     /translation="MGLVHARMARALTGQRGQGTVEYVALILLVALVMAGVVAAMKGF
                     KTDDGKELGDLIIAKIKEAVRKVQF"
     gene            complement(6929..7378)
                     /gene="rnhA"
                     /locus_tag="VFY44_12135"
     CDS             complement(6929..7378)
                     /gene="rnhA"
                     /locus_tag="VFY44_12135"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEX5911233.1"
                     /translation="MAVEVWTDGACSGNPGPGGWACVLRFNGVEKELSGGEAVSTNNR
                     MELLAVIRGLQALTRPVEVVVHLDSSYVMNAFTKNWIRGWQRNGWKNSAKKPVANREL
                     WLELLAETQRHQVTFTKVKGHSGVDLNERVDRLAVDACRRYGGKQRP"
     gene            complement(7368..7580)
                     /locus_tag="VFY44_12140"
     CDS             complement(7368..7580)
                     /locus_tag="VFY44_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911234.1"
                     /translation="MSDAREGRDPATESIRAALFKVVSTADLTDAEQAVEWARAALED
                     ETLLHSLGAGARRAAGGSAPPEPDGR"
     gene            7687..8496
                     /gene="murI"
                     /locus_tag="VFY44_12145"
     CDS             7687..8496
                     /gene="murI"
                     /locus_tag="VFY44_12145"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX5911235.1"
                     /translation="MFDSGVGGLTVLHECLVSLPHEDFVYLGDTARFPYGERSQQQLR
                     SYAHELSSVLVDRGAKLVVIACNSATAAAFETVKAELDGVVPVVGVVRPESRLAAEAT
                     TTGRVGVIATEATVASGAYDRALAEASGGAAQLTSVAAPRLAPLIQAGGEVDEATVAC
                     VEQACAPLQEARVDTVILGCTHYPLVRPLLQRTLGRGVRLITSGEAIAETVEAELDAL
                     GRSAPTTRRGAYHFLTTGEPEEFRALGTRFLQLPIAKVGHVQAQPTRIGSV"
     gene            8493..>8858
                     /gene="rph"
                     /locus_tag="VFY44_12150"
     CDS             8493..>8858
                     /gene="rph"
                     /locus_tag="VFY44_12150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX5911236.1"
                     /translation="MSRHDGRAASDLREVSIEPGFVRTADGSALIAAGKTRVICTASV
                     EEKVPRWMQHQGRGWVTAEYSMLPASTGQRKQRDVSKGRPDGRTVEIQRLIGRSLRGV
                     VDFEALGERTVWIDCDVLEA"
BASE COUNT         1086 a         3267 c         3264 g         1241 t
ORIGIN      
        1 tgggcttctg cctgttcgcc agcgtcgccg tcgccgccga gcacgcgctg acccgcgacg
       61 gggtggatcg cgtgttcgtc ctcgactggg acgtgcacca cggcaacggc acgaacgccg
      121 cgttccactc gcggcctgac gtgctcttct gcagcctgca ccagtacccg ttctaccccg
      181 gcaccggcgc tctgggcgac aacggctcgg ggccggggga ggggttcacg atcaacctcc
      241 cggtgcccgc ggggtcgggc gaggcggagt ggctcgggct ggtcgagcac gtggtcgttc
      301 ccgccgcgcg cgagttcgcg cccgacctga tcctggtctc ggccggcttc gacgcccacc
      361 gcgccgaccc cctggccgag tgcaacctgg agaccgacag ctttcgccag ctggccctgc
      421 acgtggcgtc gctcgcggcc gaactgggcg tgcccgcggg cgcggtgctg gagggcggct
      481 acgacgtgga cgcgctggcc gagtcggcgg tggccacgat ggaggcgctg gaggcgggcg
      541 ggacgccacg ctcggtcgag cgcggcccgc tggtcgacgc ggcggctagc gctctacggc
      601 ctgggtgaac gagcccgggt acttggcgtt cagccgctcg gtggcctggt cggccttgcc
      661 gcggtcctcg tagaagcccg cgaacacgac gtagaagccc tgggggagcg tcttgaagtc
      721 gtcagcgcgg atcacgccga gcttcgccgg cttcgaccgt gacgcggagc gggcgaagga
      781 cgtcgcgctg gcgcggtcct cggtcgagtt gatgaccacc gtgaacccct ccagcgtgcg
      841 cggccactgg tacagggagc ccctcttgac cagcgcgccg ctctcggccg cgggctcccc
      901 ggccttcttc ggctgctcgg ccttctcctt cgcgccggtg gtcttggtgg ccgcgggctt
      961 ggggcgcggg ggagcggcgg ccacctcgcg gtcggcgtcg tcgctcagcg cggccaccgc
     1021 catgaacgcg gccacgccca gcagcagcgc cacgaccgcg gtgatggcca ccggcacctt
     1081 ccagctcggc gggcggcggt agaccagggc cacgcgcgct ccacactcca ggcagaccag
     1141 ctggccgcgc tcggccgcgg ccccgcagcg ggggcagtgc acgtcgcccg tccggtcggt
     1201 cacgactcgt ccttgcgctt gccgcgcagc gtgcgctcca ggcggctcat cttccgcttg
     1261 accttgaccg gctcgggcgg gggcccgccc tccggtgcgt ccgaggagcg cagccaggga
     1321 gcggcgggga gcgcgccgga cgacgaccgc ggctggtcgg cggcgggctc gggttcgggc
     1381 tccggttctg gttcggcctc gggttcaggc tccggctcgg gttctggctc gggctcggcc
     1441 tcgggttccg gctcgggctc gggttccggt tctggctcgg gctcgggctc gggttccggt
     1501 tctggctcgg gttccggctc cggctccggc gcgaccgcct cggccagctc cggctcctcg
     1561 tcgaacgtct cctgctccgc gtggtcctgg gcctgcgcct cctcggcgaa caccggctcg
     1621 ggctcctcgc cgttgccctc gtgctcggtg tcctcccact gcgggtgctc ctcggtctgg
     1681 gcctcgacct ggcgctcgac cggcacctgc gcggcctggc cccgctgggc ctcgaggccg
     1741 ggcttggtgg ccgcgccgca ggcggggcag aagcgcgtgt cggtggacag cagcgcgtcg
     1801 cagttctcgc agctgccggc aatgcccgcc gccaccagct cgaccacgcc gaggtcggcc
     1861 tcgagcgcct cgctgaggcc gcgcacctcg gcgtcgacct ggctgagctc ggacgccttg
     1921 gcctggagca gctcgggctc gcggcggccg tggcggtgga gctcgaagac cagcgccccc
     1981 agatccagga gcagcgcctc gcgcacccgg cgctgctcgc ggaggcggcg gcgcatgcgg
     2041 ccgcgctccc gcgcccccgg gcgcgatgtc gctgggctcg gctgctccgg gttcggatcg
     2101 gtcattacgt gcgcggcacc atacccaggg cctgggcggt cacggccagg atcccctcgc
     2161 gtcgtttctc catcgggaac tcgtcgagca cccgctgccg ggccgcgcgg tggccctcgg
     2221 gcccgcgcgc cagcgcctcc tcgatccccg cggccaccgc cgcggggtcc tgggaggcga
     2281 tcaggacccc cgccccgtcg accacctcgg gcatcgcggt cacgtcgacc acgaccggga
     2341 cgcagccggc cagcatcgcc tcggtcacgg ccaggccgaa gccctcgtgg cgggacgcct
     2401 gcacgtacac cgacgcgctc ccgtacagcg cggtcaggtc ctcgtcggac gcccagcccg
     2461 tgaactccac gttcgggccg gcctcggccc gcagccgctc gatggcgtcg tccagcggct
     2521 tgcccacgaa cacgaagcgc acgtccggca gcagccgcga tgcctgcacg aacggcaggt
     2581 ggcccttctg gatcagcgtg cccgcgtcga tcgggcccac ggtcagcgcc agccgctcac
     2641 gcggccggga ctcgtccagc ggcccgaacg ggtcgggcac cccgtggtgg atgaccgtca
     2701 cggcctccgg cgggatcggg gtgttcgccg cgatctcgcg ctgcgacgag tacgagttgg
     2761 tcaccagccg cccggcccgc ttcatgatcc agcggctggc ccaccggcgc aggccgccct
     2821 gctggtagcc gaagccgatg tcgggcatgt tcgccgtgtc gaagccaccg acgatctgga
     2881 ccgacggctt gcgcatcagc caggccaggg tgatcgggaa gaacgtgtgc caggaggcga
     2941 accagccgaa caccaggtcg gcgcggcgca cggcgccgac caggcggggc aggttgcccc
     3001 agtggccggg ctggtaccag tcctcgatct cgtagcgctc ggccaggatc tcgcggtcga
     3061 tggccacgaa gctggccttt cgcgagtgca cgtacaggat gcgtggccgc gacacgcggc
     3121 gcatcttcgc ggatgggcct cagggacggt tcagcgggcg ctggtcggcg cgccggcacg
     3181 gtgcagcgcc gccccggcct ggcgcagcag ggtcccggag tcgctgccgt cctccggtgc
     3241 ggcggccacg cccacgctcg ggtggggcgt gggcagctcg gactccagcg cggcggcgtc
     3301 cacggcgtcg gccaggcggc ggccgaagcc ggccgcctcc tcgtcgctgc gcacgtcgtc
     3361 caggcacacc aggaactggt cgccgctcaa gcgggcgacc atgccggcct cgcccacctc
     3421 gcgccggagg gtgtcggcca cggtcatcag gacgcggtcg ccgacgtcgt ggcccaggag
     3481 gtcggtgatg cgctggaagg cgctcaactc gatcatcagc accgacaccg agcggccggt
     3541 ggccagcagg ccctcgacgt ggccctggaa cgcccgccgc gcgggaagcc cggtcagctc
     3601 gtcgcccgac aggccgagga cggcggggtc cggcggcttc gcggtcacga cgggtgtggg
     3661 gtcggccgcc tggatccgcg gggcgggcgg ccccaggtag aagccctgac cgatgtcggc
     3721 gccgagctcg cgcgcgcagc gcagctcgcc ctcggtctcg atgcccacga tgccgacggt
     3781 ggtgcccagc tcgtgggcgt aggcgatcat cgcccccacc aggtcgcggc gggcgctctc
     3841 gccctccagg cccgcggtca ggcggcgctc gagcttcacg atgtcggggc gcagctcgcg
     3901 caggtagttc acgccggcgc gctcggagcc gaggttctgg agcgcgacgc tcgcgccctc
     3961 ggcgcggaag ctctcgatca cccggcgcag gaagtcgatg tcggggcagc ggtgggcgtc
     4021 cacgatctcg aagcacacct ggtcgatgcc gatgcccacc ttgcgcgcgg tcgcccaggt
     4081 gttgcgcagg cagacctcgg ggtcgtacac cgacatcggg tcgaagttca cgaacaggcg
     4141 ctcgccgccc tccagcgccc cggcggcctg ctccagcgcc aggcggcggc tggactcgtc
     4201 cagctggcgg atgcggtcgt gggcgcgggc ggcggccacg atctggtacc cggccaccgt
     4261 ctcgtcgtcg gccacggagc cgcgcaccag ggcctcgtag gccaccgtgc ggcccgagct
     4321 caggtccacg atcggctggt aggccatgaa caggtcgtcg tcgtccagca gggccggtag
     4381 ccagggcgag cggccgcgct gcagcacgtt ctcggcgctg tcctcgtggc cgcccggctc
     4441 gcacgcgcgc accacgtcgc gctcggacgg cgtcatggcc gccagcgtct cgtacagctc
     4501 gtcgacccac atgggcccgg ccagcaccgg ttcgggctca ccgagcagcg cggccaggcg
     4561 cctgctcaca tgaggctggg tcgcgcgaag agttaccccc tgggaagaaa tcacgggttc
     4621 cctatcggtc cgcggggcct ccggcgtgag tcccgcgcgc ctagctggac gtgtgaacgg
     4681 gctcgcgcgg ctgcacgaac gcctggtgcc agtcggacag ctcgagcagg cagtacagga
     4741 ggctcttgtg gtcggcccgg ctggagcggt gctcctccac cagacgacct accgtacccg
     4801 ggtcgatcag gtcccccagc tgggttccgg gcgaataccg gcgcgcgacc tcgtctccga
     4861 gcgaggtgcg aagccagtcc tcgtacgggg tcgagaagcc gtgcttgggc cggtcgacca
     4921 ccgcccgcgg gaccagcttg cccatggcct tccggtgcag gcgcttgccg tcgcggatgc
     4981 gcacccgctc acgcgcgggc acgcgctcga cgaaccgcat cagctcgacg tccaggaacg
     5041 gcacgcgctg ttccagggaa gcggacatgg acatcttgtc ggcgcagatc aggaggctgt
     5101 ccggcaggtg caggtgggtg tccaggtaca gcgcctgctc caccagcccg cggtcgccca
     5161 cgtcggccag caccgcgtcg gcgcgctcgc gccgctcggc ggccgcctcc tggccgctgg
     5221 tgccggtcag ctcggtgcgc aggtcgtcgc cggtgatctc cagcagccgc accagccggt
     5281 cggccggctc caggtcgccc agcaggcggc tggcgcggcg cacgcgctcg ttgccgccgg
     5341 ccacgcgcgc ggccagcgag cccagcggcc cggccgcgcg gcccgcgatg cccgcggcgc
     5401 ccagcgccgc ggcggcctgg tggcggccgt agccgccgtg cggctcgtcg gcgccctggc
     5461 cggagagcac gaccttcacc gactcggcgg cgaagcgcga gagctgcatc agcgcgccgg
     5521 cggagggcag gcccacgggc tcctccagcc gccgcacgca gcgctccagc tcgcccagga
     5581 agtcgccctc ggccatggcg gtctcgtggt ggtcggtgcc cagcgtggcc gcggtctccc
     5641 gcgcgtagcg gcgctcgtcg agcacgtcgc cgtggccggg gaagccgatc gtgaaggtca
     5701 tcggagggcg gtcgctgcgc cgcgccatgg ccgcgacgat ggcggccgag tccacgcccc
     5761 cggacaggaa cgcgccgtag ggcacgtcgg acatcatctg ccgctcgacc gcgtcggtga
     5821 agcgctcggc cagctcgccc tccaggtcgc ggcccttcac gtcgagtggg tcgccggggg
     5881 cctcgcggta gctgctgacc ttcggcgccg agccccgctc gaccaccagc atgctcgccg
     5941 cgggcagctt gctgacgccg gcgaacaggg tccgcggcgc gggcacgaac cggcaggcca
     6001 ggtagtggtc cagggccacc cggtccagct cgggggtcac cagcccgccg gcgatcagcg
     6061 ccgccacctc ggacgccacc gccgtgcggg tgccgtcgct gtgccagtac aggggcttga
     6121 cgccgaacgg gtcgcgcacc gccagcagcc gctcacggcg ctggtcgtac agggcgaagg
     6181 cgaagatgcc gttcatccgg cgcacgaagt cgggtccgtg ctcctcgtac ccgtgtacca
     6241 cgacctcgga gtccgacccg gtggcgaaca cgtggccggc ccgctcgagc tcggcgcgca
     6301 ggtccaggtg gttgtagatc tcgccgttga cgatcgccgt gacctggccg tcctcgctgt
     6361 acaggggctg ctcgccgccg gccgggtcga tgatggccag gcgcgtgtgg gccagcgtcg
     6421 ccggcccgat ctggaccacg ccctcgccgt cggggccgcg atggcgcagc gcctcgcaca
     6481 tgcgctgccc cgccccgctg ggggcgctct cggccgggtc catcatcgcg ccgatgccgc
     6541 acatggggtc aatcttggcg cacggcccgg gaggagcgga gccgggcggc ggccggcgag
     6601 caccgcaccg acgctgtcac cgtctgggcg ccggatcgtc acgcccgcgt ctggagccgt
     6661 cacccgcccc ggcggacgat cgcctccact cgacgaacgg aggtggatcg atgtacggcc
     6721 tgatgggact cgtgcacgcg cggatggcgc gggcgctgac cggccagcgc ggtcagggca
     6781 cggtggagta cgtggcgctg atcttgctgg tggcgctcgt catggcgggc gtggtggccg
     6841 ccatgaaggg cttcaagacc gacgacggca aggagctcgg cgacctgatc atcgccaaga
     6901 tcaaggaggc ggtgcgcaag gtccagttct agggcctctg cttgccgccg tagcgccggc
     6961 aggcgtcgac cgcgagccgg tcgacgcgct cgttgaggtc gacgccggag tggcccttga
     7021 ccttggtgaa ggtgacctgg tggcgttgcg tctcggcaag cagctccagc cacagctcgc
     7081 ggttggcgac cggcttcttg gcgctgttct tccagccgtt gcgctgccag ccgcggatcc
     7141 agttcttggt gaaggcgttc atcacgtagc tgctgtccag gtggacgacc acctcgaccg
     7201 gccgggtcag cgcctgcagg ccgcggatga cggcgagcag ctccatgcgg ttgttggtcg
     7261 agacggcctc gccgccggag agctccttct cgacgccgtt gaagcgcagc acacaggccc
     7321 agccgccggg gcccgggttg cccgagcacg cgccgtcggt ccagacctca acggccatcg
     7381 ggctccggtg gcgcgctgcc gcccgccgcc cggcgtgctc cggcaccgag cgaatgcagg
     7441 agcgtctcgt cctccagcgc ggctcgcgcc cactcgacgg cctgctcggc gtcggtcagg
     7501 tcggcggtcg agaccacctt gaacagcgcc gccctgatcg actcggtcgc gggatcccgc
     7561 ccttctcgcg catcgctcat ggacgggggg tacccagccg gaccggggtt tgagcgccgg
     7621 gtttgcccga cggccatgcc accatggcga tccgtgcccg agcagccgcc agagcttccc
     7681 gtcgcgatgt tcgactccgg tgtgggcggc ctgacggtgc tccacgagtg cctggtctcg
     7741 ctgccccacg aggacttcgt ctacctgggc gacaccgccc ggttccccta cggcgagcgc
     7801 agccagcagc agctccgctc ctacgcccac gagctgtcgt cggtgctggt ggaccggggg
     7861 gccaagctcg tggtgatcgc ctgcaactcg gccaccgcgg cggcgttcga gaccgtcaag
     7921 gcggagctgg acggcgtcgt gcccgtggtg ggcgtggtgc ggcccgagtc gcggctggcc
     7981 gccgaggcca ccaccaccgg cagggtcggc gtgatcgcca ccgaggccac cgtggccagc
     8041 ggcgcctacg accgcgcgct ggccgaggcg tcgggcggcg ccgcgcagct gacgtcggtg
     8101 gccgcgcccc ggctcgcgcc gctgatccag gccgggggcg aggtggacga ggccacggtg
     8161 gcctgcgtcg agcaggcctg cgcgccgttg caggaggccc gggtggacac cgtgatcctg
     8221 ggctgcaccc actacccgct ggtgcggccg ctgctgcagc gcaccctggg ccgcggcgtg
     8281 cgcctgatca catcgggcga ggccatcgcg gagacggtcg aggccgagct ggacgcgctc
     8341 gggcgctccg ctcccaccac gcgccggggc gcctaccact tcctgaccac cggcgagccc
     8401 gaggagttca gggcgctggg cacccgtttc ctccagctgc cgatcgccaa ggtcgggcac
     8461 gtgcaggccc agccgacccg gatcggctcc gtatgagccg gcacgacggc cgcgccgcct
     8521 ccgacctgcg cgaggtcagc atcgagcccg ggttcgtgcg caccgccgac ggctccgcgc
     8581 tgatcgcggc cggcaagacg cgcgtgatct gcacggcctc ggtcgaggag aaggtgccgc
     8641 gctggatgca gcaccagggc cgcggctggg tcacggccga gtactcgatg ctccccgcgt
     8701 ccacgggcca gcgcaagcag cgcgacgtgt ccaagggccg gcccgacggg cgcacggtgg
     8761 agatccagcg gctgatcggc cgctcgctgc gcggggtcgt ggacttcgag gcgctgggcg
     8821 agcgcacggt gtggatcgac tgcgacgtgc tcgaggcc
//
LOCUS       DASUPW010000538         4298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1092737, whole genome shotgun sequence.
ACCESSION   DASUPW010000538 DASUPW010000000
VERSION     DASUPW010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092737"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2556)
                     /locus_tag="VFY44_12155"
     CDS             complement(<1..2556)
                     /locus_tag="VFY44_12155"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX5911237.1"
                     /translation="MPDNRLKDLQERTRYTPAEVESRVFERWEQAGVFSPEPEGTADE
                     NFSIAVPPPNVTGALHMGHALNGTYQDLFIRRARMQGRRAKWIFGTDHAGIATQVKVE
                     QALEAEGTSKEELGREAFTDRVWEWRKEYGGQINQQFRSLGASLDYADERFTMDEAYA
                     RAVQEVFVRLYEKGLVYRDNYMVNWDPGTRSAISDLEVEQRSVNDTLYEIDYPLESGS
                     GSVTVATVRPETMLADTAIAVNPADERYTRLIGERATLPLVGRSLPIIADEYVKTDFG
                     TGALKITPGHDPNDFEIGRRHGLQEISVIGEDGLMTDEAGERFAGMDPVEARKAIVEA
                     LRNEGCLSGSEPYTHDVPYSHRSGQRIEPLISLQWFCDMEQLAGPAIESVRDGRVSFH
                     PERPHTQVYLDWLENIRPWCVSRQLWWGHRIPAWYRGDEVHVGSEAPEGEGWERDPDV
                     LDTWFSSGLWPFATLGWPEQTDQLRAFYPTDVLTTGRDIIFLWVARMVMLGIEFTGEV
                     PFAHVSINSTIMAPDGRRMSKSLGTGIDPLDEIAEHGADAVRFGLVAMASTQDVRFSA
                     DRVRQGLDLANKLWNASRLILLGAKEGVEPSAELAELPEDRWILSRLERVTERTDRLI
                     ADFELSAAALELYDFFWSELCDWYLEMAKARLREGDEAVSATLLYALDRTLRLLHPLM
                     PHVTEEIWSFMPGERGLLTVAPWPEADASHRDEAAEAEVGSAIAAITAVRRYRDLVGV
                     KPSAKLPARLELGVADGLAAQIAQLARLELGEGDAVASVPVPGGTVELLAGPDFDADE
                     AGRRIEGERDRLRGEIGRLDKKLGNAKFVERAPADVVEGERAKLAEYTAALERL"
     gene            2581..3198
                     /locus_tag="VFY44_12160"
     CDS             2581..3198
                     /locus_tag="VFY44_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911238.1"
                     /translation="MGILRTSLALVAGAALLAGAPPADAAKRKPRPSCAKKGSTTVRA
                     TRDARLFTVFTRDAVDHLYGCLRAVGRPVELAEATDEIDAETAFGDVRLSGRYAAWRE
                     TSTDVSCKAACPPGYDGTTESVSVYDLRRRRTVRTVSAGVTGGALVLTRRGGVAWLSG
                     EELFAADRSGTRRLDTGAIAPRSLRAEISIVSWVRDGVERFARLR"
     gene            complement(3211..3879)
                     /locus_tag="VFY44_12165"
     CDS             complement(3211..3879)
                     /locus_tag="VFY44_12165"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5911239.1"
                     /translation="MKDVAEGVSLLRGRIPIPYAINTYLVGDVLVDAGGRTDTGVILK
                     ELRGRELSAHAITHAHPDHQGASHRVCEEFGIPFWVPELDVPPAENPDLIGERQPNHP
                     ISQLMHKVFTGPGHKVDRALKEGDEVAGFTVLDVPGHSAGHMALWRESDRTLILGDVL
                     NSQDPLLGIPGLRLPKDFFTPDPERNRQSAKRLGRLEPLLVLFGHGPPVRDTRKFVDF
                     CAKL"
     gene            complement(3876..>4298)
                     /locus_tag="VFY44_12170"
     CDS             complement(3876..>4298)
                     /locus_tag="VFY44_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911240.1"
                     /translation="AGSGRVLPLGLSRADVAALVAVLSIGLTLAAARRGGGRTLERAL
                     ALLALVLLLRCALDPAVLQYYTVGPMLALLCWEVAARPGLPVFALLYALSSWAVYGPL
                     LSGNSDALVSAAQVALIALFASLMAGAAFGRAPVASRA"
BASE COUNT          574 a         1579 c         1556 g          589 t
ORIGIN      
        1 gaggcgttcc agggccgccg tgtactcggc cagcttggcg cgctcgccct cgaccacgtc
       61 ggcgggcgcg cgctccacga acttcgcgtt gcccagcttc ttgtccagcc gcccgatctc
      121 gccccgcagg cggtcgcgct cgccctcgat ccgccggccg gcctcgtcgg cgtcgaagtc
      181 gggccccgcc agcagctcga ccgtgccgcc gggcaccggg acgctggcca ccgcgtcccc
      241 ctcccccagc tccagccgcg ccagctgggc gatctgcgcg gccagcccgt cggccacgcc
      301 cagctccagc cgggccggca gcttggccga gggcttgacc cccaccagat cgcggtagcg
      361 gcgcacggcc gtgatggcgg cgatcgcgct gccgacctcg gcctccgcgg cctcgtcccg
      421 gtgcgacgcg tccgcctcgg gccacggggc caccgtcagc agaccgcgct ccccgggcat
      481 gaacgaccag atctcctcgg tgacgtgcgg catcagcggg tgcagcaggc gcagcgtgcg
      541 gtccagcgcg tagagcagcg tggccgagac ggcctcgtcg ccctcgcgca ggcgcgcctt
      601 ggccatctcc aggtaccagt cgcacagctc cgaccagaag aagtcgtaca gctcgagcgc
      661 ggccgccgac agctcgaagt cggcgatcag gcggtcggtg cgctcggtca cccgctccag
      721 ccgcgacagg atccagcggt cctcgggcag ctcggcgagc tccgctgacg gctcgacccc
      781 ctccttggcg cccagcagga tcagccgcga ggcgttccag agcttgttgg ccaggtccag
      841 gccctggcgc acgcggtcgg cggagaagcg cacgtcctgg gtcgaggcca tggccaccag
      901 gccgaagcgg acggcgtcgg cgccgtgctc ggcgatctcg tccagcgggt cgatgccggt
      961 gcccagcgac ttggacatgc ggcggccgtc gggggccatg atcgtggagt tgatggacac
     1021 gtgggcgaac ggcacctcgc ccgtgaactc gatgcccagc atcaccatgc gggccaccca
     1081 caagaagatg atgtcccggc ccgtggtgag cacgtcggtg gggtagaacg cgcgcagctg
     1141 gtcggtctgc tcgggccagc ccagggtggc gaacggccac aggcccgacg agaaccaggt
     1201 gtccagcacg tcggggtcgc gctcccagcc ctcgccctcg ggcgcctcgc tgcccacgtg
     1261 gacctcgtcg ccgcggtacc aggcggggat ccggtggccc caccacagct ggcgcgacac
     1321 gcaccagggg cggatgttct ccagccagtc caggtagacc tgggtgtggg gacgctcggg
     1381 gtggaaggac acgcggccgt cgcgcaccga ctcgatcgcg ggaccggcca gctgctccat
     1441 gtcgcagaac cactgcagcg agatcagggg ctcgatccgc tggccggagc ggtgcgagta
     1501 gggcacgtcg tgggtgtagg gctccgagcc cgacaggcag ccctcgttgc gcagggcctc
     1561 cacgatcgcc ttgcgcgcct ccacggggtc catgccggcg aaccgctccc ccgcctcgtc
     1621 ggtcatcagg ccgtcctcgc cgatcacgga gatctcctgc agcccgtggc ggcggccgat
     1681 ctcgaagtcg ttggggtcgt gccccggggt gatcttcagc gcgcccgtgc cgaagtcggt
     1741 cttcacgtac tcgtcggcga tgatgggcag cgaccggccc accagcggca gcgtggcgcg
     1801 ctcgccgatc agccgggtgt agcgctcgtc ggccgggttc accgcgatgg cggtgtcggc
     1861 cagcatcgtc tcgggccgca cggtggccac ggtgaccgac cccgaccccg actccagcgg
     1921 gtagtcgatc tcgtacagcg tgtcgttcac gctgcgctgc tcgacctcga ggtccgagat
     1981 ggccgagcgg gtgcccgggt cccagttgac catgtagttg tcgcggtaga ccaggccctt
     2041 ctcgtacagg cgcacgaaca cctcctgcac ggcgcgggcg taggcctcgt ccatggtgaa
     2101 gcgctcgtcg gcgtagtcca gcgaggcgcc caggctgcgg aactgctggt tgatctggcc
     2161 gccgtactcc ttgcgccact cccacacccg gtcggtgaac gcctcgcggc ccagctcctc
     2221 cttgctggtg ccctcggcct ccagcgcctg ctcgaccttg acctgggtgg cgatgccggc
     2281 gtggtcggtg ccgaagatcc acttggcccg ccgcccctgc atccgcgccc ggcggatgaa
     2341 caggtcctgg taggtgccgt tcagggcgtg gcccatgtgc agggccccgg tcacgttggg
     2401 cggcggcacg gcgatcgaga agttctcgtc ggccgtgccc tccggctcgg ggctgaacac
     2461 cccggcctgc tcccagcgct cgaacacgcg cgactccacc tcggcgggcg tgtagcgcgt
     2521 gcgctcctgc aggtccttga ggcggttgtc cggcacggcg ggggagtcta gagtcggcca
     2581 atggggatcc tccggacgtc cctggcgctc gtggccggcg cggccctgct ggccggagcc
     2641 ccaccggccg acgcggccaa gcgcaagccc aggccgtcgt gtgcgaagaa ggggtcgacg
     2701 accgtgcggg cgacccgcga cgcgcgcctg ttcacggtgt tcacccgcga cgccgtcgac
     2761 cacctctacg gctgcctgcg ggcggtgggc cgcccggtcg agctggccga ggcgaccgac
     2821 gagatcgacg ccgagaccgc gttcggcgac gtccgcctga gcggccggta cgccgcgtgg
     2881 cgcgagacca gcaccgacgt cagctgcaag gcggcgtgcc cgccgggcta cgacggcacc
     2941 accgagtccg tgagcgtcta cgacctgcgc cgccggcgca cggtgcgcac ggtgtccgcg
     3001 ggcgtcaccg gcggggcgct ggtcctgacc cggcgcggcg gggtggcgtg gctgtcgggg
     3061 gaagagctgt tcgcggccga ccgctccggc acgcggcgcc tggacaccgg cgcgatcgcg
     3121 ccccgctcgc tgcgcgccga gatctcgatc gtctcgtggg tgcgcgacgg ggtcgagcgc
     3181 ttcgcgcgcc tgcgctgaga gcccggcgcg ctagagcttc gcgcagaagt ccacgaactt
     3241 gcgcgtgtcg cgcacgggcg ggccgtgccc gaacagcacc agcagcggct ccagccggcc
     3301 caggcgcttg gccgactggc ggttgcgctc ggggtccggc gtgaagaagt ccttgggcag
     3361 gcgcagcccc gggatcccca gcagggggtc ctggctgttc agcacgtcgc ccaggatcag
     3421 cgtgcggtcg gactcgcgcc acagcgccat gtggccggcg gagtggccgg gcacgtccag
     3481 caccgtgaag ccggcgacct cgtcgccctc cttcagcgcg cggtcgacct tgtggccggg
     3541 gccggtgaac accttgtgca tcagctggga gatcgggtgg ttgggctggc gctcgccgat
     3601 caggtcgggg ttctcggccg gcggcacgtc cagctcgggc acccagaacg ggatcccgaa
     3661 ctcctcgcac acgcggtggc tggcgccctg gtggtcgggg tgggcgtggg tgatggcgtg
     3721 ggccgacagc tcgcgcccgc gcagctcctt gaggatcacg ccggtgtcgg tgcgcccgcc
     3781 cgcgtccacc agcacgtcgc ccaccaggta ggtgttgatg gcgtacggga tcgggatccg
     3841 gccgcgcagg agcgatacgc cttcggccac gtctttcatg cgcgcgaggc taccggggcg
     3901 cgcccgaacg cggcgccggc catcagagag gcgaacaggg cgatcagggc cacctgggcg
     3961 gcgctgacca gcgcgtccga gttgccggac agcagcgggc cgtagaccgc ccagctgctc
     4021 agcgcgtaca gaagcgcgaa caccggcaac cccggccgcg ccgccacctc ccagcacagc
     4081 agcgcgagca tgggccccac ggtgtagtac tgcagcaccg cggggtccag cgcgcagcgc
     4141 agcagcaaca ccagggccag gagcgcgagt gcgcgctcga gcgtgcgccc gcctccgcgc
     4201 cgggccgcgg ccagcgtgag cccgatggac agcacggcca ccagcgcggc cacgtccgcg
     4261 cggctcagcc ccagcggcag gacgcgaccc gacccggc
//
LOCUS       DASUPW010000539         5388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1093336, whole genome shotgun sequence.
ACCESSION   DASUPW010000539 DASUPW010000000
VERSION     DASUPW010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093336"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="VFY44_12175"
     CDS             complement(<1..976)
                     /locus_tag="VFY44_12175"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX5911241.1"
                     /translation="MATQGTTVQIEMPTVGESVNEGIVLEWLVKVGDTVAEGDDLVEM
                     STDKVDAPVPSTVAGVVTKLLVEVDETVTVGQALCEIEAGDAPAAAEPKPSENEPSEN
                     GSSDAADGADGGGSGEIVDVTLPEMGDSVVEGTLLEWLVKAGDQVSEGDDLAEMSTDK
                     IDAALPAPVSGTVVELLAEADDTVAVGAVLCRIEAGAGAAPSGKVDAPAEKTAAKPEA
                     GAPATQGTPSPTGNGDANATPVAARFAADQGVDTSKLTGTGPRGRVTKADVQNAAAGG
                     GNGASAKPAAPPAPVPEGAETTVIRGPAATLARFMDMSRSIPTATSFRT"
     gene            1053..2075
                     /locus_tag="VFY44_12180"
     CDS             1053..2075
                     /locus_tag="VFY44_12180"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5911242.1"
                     /translation="MTDDRYRAGPRRRKELARADKVLPGVWRLRLPLPWPGVPHGNAW
                     AVAAGDGVVLFDTGMDQPGALGQLELALGQAGLRLEHVRLLVCTHAHSDHYGLAGPII
                     DASGCELWMHPDDEHMRKLVEDPDAQLNRRLEVALHSGVPLESVEAAREARTGTGSGI
                     ARLVEPDKHLLPGVEVDTDLGRWQVYETPGHAPSHVVLHQPDRRLMISGDHIIGRVSL
                     YYDFGWTPDPAGEFLTSLDTVEGLPTDLILPGHGKPVRDLPGLVRANREAVHERMGRV
                     RDAIAGAELTPFEIVPKLLQTEDLNPMLVNWGLAETLAYLRHLEVEGQAEKVEGEDPE
                     RWKLTG"
     gene            complement(2134..3033)
                     /gene="metF"
                     /locus_tag="VFY44_12185"
     CDS             complement(2134..3033)
                     /gene="metF"
                     /locus_tag="VFY44_12185"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEX5911243.1"
                     /translation="MRIDETIERLDRPVFSFEFFPPKTDEGFANLRTTLDALRNDQPD
                     FVSVTYGALGTTRDRTLDIVKWIKQDLDIEAMAHFTCVGATREELSETLREMADSGVD
                     NVLALRGDPPQGEDEWKPTEGGLLYSTELIELLQGEFDFAVGAAAFPEVHPEAESAKS
                     DLEFLKAKQEAGASFFITQLFFDNEFYFDFVAKARDMGITVPIIPGIMPVTSVKNIKR
                     ITELCKSEIPPAFERELDRREDDTEAVEDLGVAYSTLQCVDLLSRGAPGIHFYTLNRS
                     PATRAILAALRAAQPWTRAWQPV"
     gene            3058..4299
                     /locus_tag="VFY44_12190"
     CDS             3058..4299
                     /locus_tag="VFY44_12190"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX5911244.1"
                     /translation="MPLIADTPPREVIAHSERGRAVVARRIGSAGAQRRILVVGAIHG
                     NERAGVPVLRALRSARPPRGVQIVLVDTVNPDGDARGTRQNARGVDLNRNWPVAWRGG
                     GRPFGTYFPGTAPLSEPESRGLSAYITRLRPRVTLYYHQHMRLVDRSGGDGFLTRLYA
                     RASRLPWRGIDPLPGTATRWQNRTHPGDTAFVVELPAGRLSRAGVRRHRGAVLALARA
                     VAPPPVRSTPIPYGAERRAQMRAYARRHYGLDTDVLKPRVIVQHFTASNSFQSAFQTF
                     ARNQRDAELGELPGVCAHYLIDRDGTIHRLVRDMLMCRHTVGLNWAAIGIEHVGASDG
                     QVLGNARQRRASLRLTRSLQGRYGIATKNVIGHAESLSSPFHREKVARLRRQTHGDFR
                     RASMRRYRAALGRLPAARSLR"
     gene            4360..4842
                     /locus_tag="VFY44_12195"
     CDS             4360..4842
                     /locus_tag="VFY44_12195"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5911245.1"
                     /translation="MGPGDGKAVDLGSIGARIMAWTEETGAGFSLVEHPMPPRALAAP
                     LHKHSREDEYSFVLEGRMGALLGDDVVYAEVGDLAFKPREQWHTFWNAGDEPCRILEI
                     ISPGGFEHFFDELGTLTAADADPAELGALAARYGLELQPESVPRLCEEHGLVFPEPPG
                     "
     gene            complement(4847..5161)
                     /locus_tag="VFY44_12200"
     CDS             complement(4847..5161)
                     /locus_tag="VFY44_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911246.1"
                     /translation="MADATDDGGYRGNRVPLPKGAQPPLRVFVNGVRQQEGADFSVEG
                     DELVFTRELAKERVGFWRWTAMFFALFGSYGRNDTVDVHYKVGGRDQVAAGLQIVPPE
                     ES"
     gene            complement(5170..>5388)
                     /locus_tag="VFY44_12205"
     CDS             complement(5170..>5388)
                     /locus_tag="VFY44_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911247.1"
                     /translation="GTRAVGGRLMRRFEPVQQVVGRIELKGPGVSCGVDVRGDGSAEA
                     FTGKVRRSLVHQQPGESPYDALARELGV"
BASE COUNT          701 a         1952 c         1974 g          761 t
ORIGIN      
        1 gcgtgcggaa gctcgtggcg gtcgggatcg accggctcat gtccatgaac cgggccaggg
       61 tggcggcggg gccgcggatc acggtggtct cggcgccctc gggcaccggg gccggcggcg
      121 cggccggctt ggcggaggcg ccgttgccgc cgccggcggc agcgttctgc acgtcggcct
      181 tggtcacgcg cccgcgcggg cccgtgccgg tcagcttgga ggtgtccacg ccctggtcgg
      241 cggcgaaccg ggccgccacg ggggtggcgt tggcgtcgcc gttgccggtg gggctgggtg
      301 tgccctgggt ggcgggcgcg ccggcctcgg gcttggcggc ggtcttctcg gccggcgcgt
      361 cgaccttgcc ggacggggcg gctccggccc cggcctcgat ccggcacagc acggcgccga
      421 cggccacggt gtcgtcggcc tcggccagca gctcgaccac ggtgcccgaa acgggcgcgg
      481 ggagcgcggc gtcgatcttg tccgtggaca tctcggccag gtcgtcgccc tcggagacct
      541 ggtccccggc cttgaccagc cactccagca gggtgccctc gaccacggag tcgcccatct
      601 cgggcagggt cacgtccacg atctcgccgg agccgccgcc gtccgcgccg tccgcggcgt
      661 ccgacgagcc gttctcggag ggctcgttct cggacggctt gggctccgcg gccgcggggg
      721 cgtcgccggc ctcgatctcg cacagggcct ggcccacggt cacggtctcg tcgacctcga
      781 ccagcagctt ggtgaccacg ccggccaccg tggagggcac gggggcgtcc accttgtcgg
      841 tggacatctc gaccagatcg tcgccctcgg cgaccgtgtc accgaccttg accagccatt
      901 cgaggacgat gccttcgttc accgactccc cgacggtcgg catctcgatc tgaacggtgg
      961 ttccctgggt ggccatctac tgcaccacca ctctacatac gggaacttgc gggactgtca
     1021 cggtccgttc cccgtgaaag agtggttgcg tcatgaccga cgatcgctac cgcgccggac
     1081 cccgtcggcg caaggagctg gcccgggccg acaaggtcct tcccggcgtc tggcggctac
     1141 gcctgccgct gccctggccg ggggtcccgc acggcaacgc ctgggcggtg gcggcgggcg
     1201 acggggtggt gctgttcgac accggcatgg accagccggg cgccctcggc cagctggagc
     1261 tggcgctggg ccaggccggg ctgaggctgg agcacgtgcg gctgctggtg tgcacccacg
     1321 cccactccga ccactacggg ctggccgggc cgatcatcga cgcctcgggg tgcgagctgt
     1381 ggatgcaccc cgacgacgag cacatgcgca agctggtcga ggaccccgac gcgcagctga
     1441 accggcggct ggaggtggcg ctgcacagcg gggtgccgct ggagtcggtc gaggccgcac
     1501 gtgaggcgcg gaccggcacc ggcagcggca tcgcccggct ggtggagccc gacaaacacc
     1561 tgctcccggg cgtggaggtg gacaccgacc tgggccgctg gcaggtgtac gagacaccgg
     1621 ggcacgcgcc ctcgcacgtg gtcctgcacc agcccgaccg caggctgatg atctcggggg
     1681 accacatcat cggccgcgtg tcgctgtact acgacttcgg ctggaccccg gacccggcgg
     1741 gcgagttcct gaccagcctg gacacggtcg agggcctgcc caccgacctg atcctgcccg
     1801 gccacggcaa gccggtgcgc gacctgccgg ggctggtgcg ggccaaccgc gaggccgtgc
     1861 acgagcgcat ggggcgcgtg cgcgacgcca tcgccggcgc cgagctgacg ccgttcgaga
     1921 tcgtgcccaa gctgctccag accgaggacc tgaacccgat gctcgtgaac tggggcctgg
     1981 ccgagacgct ggcctacctg cgccacctcg aggtcgaggg ccaggccgag aaggtcgagg
     2041 gcgaggaccc cgagcgctgg aagctgaccg gctagagcga ccggccaagg gtcgcggggg
     2101 gtgcgggggg cgtgagcccc ctgcgcggca tcctcagacg ggctgccacg cgcgcgtcca
     2161 cggctgggcc gcgcgcaggg ccgccaggat cgcccgcgtc gcgggggagc ggttcagcgt
     2221 gtagaagtgg atcccggggg cgccgcgcga gagcaggtcc acgcactgga gcgtcgagta
     2281 ggccacgccc aggtcctcga ccgcctcggt gtcgtcctcg cgccggtcca gctcgcgctc
     2341 gaacgcgggc gggatctcgg acttgcacag ctcggtgatg cgcttgatgt tcttcaccga
     2401 ggtcacgggc atgatgcccg ggatgatcgg cacggtgatg cccatgtccc gcgccttggc
     2461 cacgaagtcg aagtagaact cgttgtcgaa gaagagctgg gtgatgaaga agctcgcgcc
     2521 cgcctcctgc ttggccttca ggaactccag gtcggacttg gcgctctcgg cctccgggtg
     2581 cacctcgggg aaggcggcgg cgccgacggc gaagtcgaac tcgccctgga gcagctcgat
     2641 cagctcggtc gagtacagca gcccgccctc ggtgggcttc cactcgtcct cgccctgggg
     2701 cgggtcgccg cgtagcgcca gcacgttgtc cacgcccgag tcggccatct cccgcagggt
     2761 ctcggagagc tcctcgcgcg tggcgcccac gcaggtgaag tgggccatcg cctcgatgtc
     2821 caggtcctgc ttgatccact tgacgatgtc cagcgtgcgg tcgcgcgtgg tgcccagcgc
     2881 gccgtaggtc accgagacga agtccggctg gtcgttgcgc agggcgtcga gcgtggtccg
     2941 caggttggcg aacccctcat cggtcttggg cgggaagaac tcgaaggaga agaccggccg
     3001 gtccagccgc tcgatggtct cgtcgatgcg catccgcgga agggtacttt cccgggcatg
     3061 cccttgatcg cagacacgcc cccgcgcgag gtgatcgccc attccgagcg gggcagggcg
     3121 gtggtcgcgc gccggatcgg gagcgccggc gcgcagcgcc ggatcctggt ggtgggggcc
     3181 atccacggca acgagcgcgc gggcgtcccg gtgctgcggg cgctgcgctc ggcccgcccg
     3241 ccgcgcgggg tccagatcgt gctggtggac acggtcaacc ccgacggcga cgcgcggggc
     3301 acgcgccaga acgcgcgcgg ggtcgacctg aaccgcaact ggccggtggc ctggaggggc
     3361 ggcgggcgcc ccttcggcac ctacttcccc gggacggcgc cgctgtccga gcccgagagc
     3421 cgcggcctct cggcgtacat cacgcgcctg cgcccgcgcg tgacgctgta ctaccaccag
     3481 cacatgcggc tggtggaccg ctcgggtggc gacggcttcc tcacgcgtct gtacgcccgc
     3541 gccagccgcc tgccctggcg cggcatcgac ccgctgcccg gcaccgcgac gcggtggcag
     3601 aaccgcaccc acccgggcga caccgcgttc gtggtcgagc tgccggccgg gcgcctgtcg
     3661 cgcgccggtg tgcgtcgcca ccggggcgcg gtgctcgcgc tggcccgcgc ggtcgccccg
     3721 ccgcccgtgc gctccacccc gatcccctac ggcgccgagc ggcgcgcgca gatgcgggcc
     3781 tacgcgcgcc gccactacgg cctggacacg gacgtgctga agcccagggt catcgtccag
     3841 cacttcacgg cgtcgaacag cttccagtcg gcgttccaga ccttcgcccg caaccagcgc
     3901 gacgccgagc tgggcgagct gcccggggtg tgcgcgcact acctgatcga ccgcgacggc
     3961 accatccacc ggctggtccg cgacatgctg atgtgccgcc acacggtcgg gctgaactgg
     4021 gcggccatcg ggatcgagca cgtgggcgcc agcgacggcc aggtgctggg caacgcccgc
     4081 cagcgcaggg cgtcgctgcg gctgacccgg tcgctccagg gccgctacgg catcgcgacg
     4141 aagaacgtga tcggccacgc cgagagcctg tccagcccct tccaccgcga gaaggtggcc
     4201 cggctgcggc gccagaccca cggcgacttc cggcgggcgt cgatgcggcg ctaccgggcc
     4261 gcgctgggac ggttgcccgc tgcacgctcg ctgaggtagg ttcggtccat gggtgctggt
     4321 cacctggagc gtttcgatgg ttcgcagggt cctcgcatcg tgggccccgg cgacggcaag
     4381 gcggtcgacc tgggctcgat cggcgcccgg atcatggcct ggaccgagga gacgggcgcc
     4441 gggttctccc tggtcgagca cccgatgcct ccccgggcgc tggccgcccc gctgcacaag
     4501 cactcgcgtg aggacgagta ctccttcgtg ctcgaaggcc gcatgggcgc cctgctgggt
     4561 gacgacgtgg tctacgccga ggtgggcgac ctggcgttca agccgcgtga gcagtggcac
     4621 accttctgga acgccggcga cgagccgtgc cggatcctcg agatcatctc gcccgggggc
     4681 ttcgagcact tcttcgatga gctcgggacg ctcacggcgg cggacgccga ccccgccgag
     4741 ctgggcgcgc tggccgctcg ctacggactg gagctccagc ccgagagcgt gccgcggctg
     4801 tgcgaggagc acgggctcgt cttccccgag ccgcccggct agagcctcag ctctcctccg
     4861 gcggcacgat ctgcaggccg gccgccacct ggtcgcgccc gccgaccttg tagtgcacgt
     4921 cgacggtgtc gttgcgcccg tagctgccga acagggcgaa gaacatggcc gtccagcgcc
     4981 agaagcccac gcgctccttg gccagctcgc gcgtgaacac cagctcgtcg ccctcgacgg
     5041 agaagtcggc gccctcctgc tggcggaccc cgttcacgaa cacgcgcagc ggcggctggg
     5101 cgcctttggg caggggcacg cggttcccgc ggtacccgcc gtcgtcggtc gcgtcggcca
     5161 tgcccggcgt tataccccca gctcccgggc cagggcgtcg tagggggact cgcccggctg
     5221 ctggtgcacc agcgagcggc gcaccttgcc ggtgaacgcc tcggccgagc cgtcgccgcg
     5281 cacatcgacg ccgcagctca cgccggggcc cttgagctcg atccgcccca ccacctgctg
     5341 gaccggttcg aagcggcgca tcagccggcc ccccacggcc cgggtgcc
//
LOCUS       DASUPW010000540         3082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_124129, whole genome shotgun sequence.
ACCESSION   DASUPW010000540 DASUPW010000000
VERSION     DASUPW010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124129"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1234)
                     /locus_tag="VFY44_12210"
     CDS             complement(<1..1234)
                     /locus_tag="VFY44_12210"
                     /inference="COORDINATES: protein motif:HMM:NF039196.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX5911248.1"
                     /translation="MDTLDTEVLLQVLSEVRAGDFTARMPAHWTGTAGKVADSLNDII
                     SANQALGSEVERVSRVVGKEGKLSQRMSFKGTDQVWGDCIESVNSLIEDLVRPSNEMQ
                     RVIGAVADGDLSKKVSADVQGEMLELKNTINGMVDQLNGFISEVTRVAREVGTEGRLG
                     QAAAYTIEVGGVWKELTDNVNLMAGNLTGQVRDIAEVTTAVANGDLSKKITADVRGEF
                     LELKNTVNAMVDQLNRFAGEVTRVAREVGVEGKLGGQAQSQEVAGVWKDLTDDVNQLA
                     ANLTNQVRAIAEVATAVTDGDLTRQVSVEASGEVAALKDLLNEMIRNLRETMRQNTEQ
                     DWLKTNLERFTRRLQGQRELATVSNMILSELAPLVSAQHGVFYALTHEDGTEPMLRFQ
                     AGYGYRERKHLLTSFRIGE"
     gene            1444..2646
                     /locus_tag="VFY44_12215"
     CDS             1444..2646
                     /locus_tag="VFY44_12215"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5911249.1"
                     /translation="MLSTAQLPEAAIVIDDQGTVHEGNALGAECLGVAARELPGRLLA
                     DLMPDAGWVTEAIDATPADGIWRAAIQGRRRDGTPFPLDASGRRIEDGPPARYLVTLR
                     DRNVDGSLASLAMHYFDAAFDQSPLGMALYSTDGHFVRVNDAMTGMLGRDREELLGMR
                     DQELTHPDDRRADLEAAELILAGARSSHQTEKRFLRPDGQEVWVIANLSFLRDEHGRP
                     ISWLGQFQDVTEHRALAERDMLTQLYNRRRFGEALAESLSHSARYHPSGSLVLFDLDG
                     FKAVNDGHGHAAGDQVLTAIARAVTRRARDTDVVARLGGDEFAVILPHTDADGAERFA
                     RSLVGLVKALRFDFAPDEPLVTVSVGVAPFADETDPEPVVGAADRALYEVKRAGGDGF
                     AVKAPVAS"
     gene            complement(2643..>3082)
                     /locus_tag="VFY44_12220"
     CDS             complement(2643..>3082)
                     /locus_tag="VFY44_12220"
                     /inference="COORDINATES: protein motif:HMM:NF012928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5911250.1"
                     /translation="LHRMDPGAAGLHLLPPLAPGGVIELTTTADTPPETLARARELAA
                     ALGQHAELVGDAPGLVLGRIACQLVNEAAFLIGEGNGTAEDVDAGLELGVNHPRGPVA
                     WSDDIGLAHVTTVLGALRRERGEAYRTAPLLAQRAALGAGLAG"
BASE COUNT          385 a         1122 c         1098 g          477 t
ORIGIN      
        1 cctcgccgat ccggaacgag gtcaggaggt gcttgcgctc gcggtagccg tagccggcct
       61 ggaacctcag catcggctcc gtcccgtcct cgtgggtcag ggcgtagaag accccgtgct
      121 gcgcggacac cagcggcgcc agctccgaga ggatcatgtt ggacacggtg gccagctcgc
      181 gctgtccctg gagcctgcgc gtgaagcgct cgaggttggt cttcagccag tcctgctcgg
      241 tgttctggcg catggtctcg cgcaggttgc ggatcatctc gttgagcagg tccttgagcg
      301 ccgcgacctc gccgctggcc tccacgctga cctggcgcgt caggtcgccg tcggtgacgg
      361 cggtggccac ctcggcgatg gcgcgcacct ggttggtcag gttggccgcg agctggttga
      421 cgtcgtcggt caggtccttc cagacgccgg ccacctcctg cgactgggcc tggcctccga
      481 gcttgccctc gaccccgacc tcgcgggcca cgcgggtcac ctcgcccgcg aaccggttca
      541 gctggtcgac catcgcgttg accgtgttct tgagctccag gaactcgccg cgcacgtccg
      601 cggtgatctt cttgctcagg tcgccgttgg cgaccgcggt ggtgacctcg gcgatgtcgc
      661 gcacctgacc cgtcaggttg cccgccatca ggttgacgtt gtcggtcagc tccttccaga
      721 ctccgccgac ctcgatcgtg tacgcggcgg cctggcccag cctgccctcg gtgccgacct
      781 cccgggccac gcgggtgacc tcggagatga agccgttgag ctggtcgacc atcccgttga
      841 tggtgttctt gagctccagc atctcgccct ggacgtcggc cgagaccttc ttgctcaggt
      901 cgccgtccgc gacggcgccg atcacgcgct gcatctcgtt gctcggtcgg acgaggtcct
      961 cgatcaggct gttcacggac tcgatgcagt cgccccacac ctggtcggtg cccttgaagg
     1021 acatgcgctg ggagagcttg ccctccttgc cgaccaccct gctgacgcgc tcgacctcgc
     1081 tgcccagcgc ctggttggcg ctgatgatgt cgttcaggct gtcggccacc ttgcccgcgg
     1141 tgccggtcca gtgcgcgggc atgcgcgctg tgaagtcgcc ggcgcggacc tcgctgagca
     1201 cctggagcag gacctcggtg tcgagggtgt ccacgggcgg tgcgtcctgg gtcggcgtga
     1261 ggcccaatgg gctcctctat gtgagggcgg tcgcgcagtc gcgcggtcac cgggctggcg
     1321 aaatccgtcc gccggtacgt aggcgaggaa gtccctcgct gctccttgca tcggcccggg
     1381 tgccccgcag cattagtgcc gggtccggcg gaggcctggt acgcgggcgt atatcgtgcc
     1441 cgcgtgctct ccaccgcgca gctgccggag gccgccatcg tcatcgacga ccagggcacc
     1501 gttcacgagg gcaacgcgct gggtgccgag tgcctgggcg tggcggccag ggagcttccg
     1561 gggcgcctgc tggccgacct gatgcccgac gcgggctggg tcaccgaggc catcgacgcc
     1621 acgccggccg acgggatctg gcgggcggcc atccagggcc ggcgccgcga cggcaccccg
     1681 ttcccgctgg acgccagcgg ccgccggatc gaggacggcc ccccggcgcg gtacctggtc
     1741 accctgcgcg accgcaacgt ggacgggagc ctggccagcc tggccatgca ctacttcgac
     1801 gccgcgttcg accagtcccc gctgggcatg gcgctgtaca gcaccgacgg ccatttcgtg
     1861 cgcgtgaacg acgccatgac cgggatgctc gggcgcgacc gcgaggagct gctgggcatg
     1921 cgcgaccagg agctcaccca cccggacgac cgtcgggcgg acctggaggc ggccgagctg
     1981 atcctggccg gcgcgcggtc gagccaccag accgagaagc ggttcctccg tcccgacggc
     2041 caggaggtgt gggtcatcgc caacctgagc ttcctccgtg acgagcacgg ccggccgatc
     2101 agctggctgg gccagttcca ggacgtgacc gagcaccgcg cgctggccga gcgcgacatg
     2161 ctcacccagc tctacaaccg gcgccggttc ggggaggcgc tggcggagag cctcagccac
     2221 agcgcccgct accacccgtc gggctcgctg gtgctgttcg acctggacgg cttcaaggcg
     2281 gtcaacgacg gccacgggca cgccgccggg gaccaggtgc tgaccgcgat cgcccgggcg
     2341 gtgacgcggc gggcccgcga caccgacgtc gtggcccgcc tgggcggcga cgagttcgcg
     2401 gtgatcctgc cgcacaccga cgccgacggg gccgagaggt tcgcccgctc gctggtcggg
     2461 ctggtcaagg ccctgcgctt cgacttcgcc cccgacgagc cgctggtcac ggtcagcgtg
     2521 ggcgtggcgc cgttcgccga cgagaccgac cccgagccgg tggtcggcgc cgccgaccgt
     2581 gcgctctacg aggtcaagcg agcgggcggc gacggcttcg ccgtcaaggc gccggtcgcc
     2641 tcctagccgg ccaggccggc gcccagcgcc gcccgctgcg ccagcagcgg ggccgtgcgg
     2701 taggcctcgc cccgctcgcg acggagcgcg cccagcacgg tggtcacgtg ggccaggccg
     2761 atgtcgtccg accaggcgac cggtccgcgc gggtggttca cgcccagctc gagcccggcg
     2821 tccacgtcct cggccgtgcc gttgccctca ccgatcagga acgccgcctc gttgaccagc
     2881 tggcaggcga tccgtcccag caccaggcct ggcgcgtcac ccaccagctc ggcgtgctgg
     2941 cccagcgcgg cggccagctc acgcgcccgg gccagggtct ccggcggcgt gtccgcggtg
     3001 gtggtcagct cgatcacgcc tccgggggcc agcgggggca gcaggtgcag gcccgccgcg
     3061 cccgggtcca tgcggtgcag cg
//
LOCUS       DASUPW010000541         2038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_264041, whole genome shotgun sequence.
ACCESSION   DASUPW010000541 DASUPW010000000
VERSION     DASUPW010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264041"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1485)
                     /locus_tag="VFY44_12225"
     CDS             complement(<1..1485)
                     /locus_tag="VFY44_12225"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain-containing protein"
                     /protein_id="HEX5911251.1"
                     /translation="MAGLGATAVAAVITSTSVQGEGAALAGLARASMVAAPIGVGLYA
                     WHSRPDDRFGRLLVAAGFGWFLTTFAETSDELLYSIGRVAGWAVEVGLIGLILAFPSG
                     RLTARADRLLLGAAAAIFAVLYLPTTLLVETFPVPSQFTSCHGGCPGNAFQIGTEPAF
                     LGDVVVPLREALTTLVFLAVTARVAQRVRGAGPLTRLTLLPVLVVAATRWGLLALGIV
                     LRRAAPDSPLVEAVVWAAALAVPAVAVAFLVGLLQRRLYEAGALQQLGTAARGAADRE
                     QLRAVLSTAVGDPSLELVYRAEGPPRWVDGQGRPSGPPGRGRCLTEVRQNGHAVVGLV
                     HEEALLEEADFLEAASSYALTALENRRLSAQVEASLDEVRRSRTRIQASGDRERRRIE
                     RDLHDGAQQRLVALGIQLELTEDLIRDDRERGLERLHALGEELGRTLEEIQALGRGVY
                     PSLLADRGLGEALRAAALRLPLAATVEADGAGRYPAEVENAVYFC"
     gene            complement(1632..>2038)
                     /locus_tag="VFY44_12230"
     CDS             complement(1632..>2038)
                     /locus_tag="VFY44_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911252.1"
                     /translation="EERISEAAGLNSASGSFGLSFGLAVAGGVLLATLSLAFTNMSNA
                     SPEIPSSQQRQIAQTLEDDAQVISTSDLDERLADEPEAVREEVLGINADATNLALQVA
                     LLIPILAGFLGLLSAGRMVRLTRAEPVVARSP"
BASE COUNT          292 a          798 c          716 g          232 t
ORIGIN      
        1 gcagaagtag accgcgttct cgacctccgc ggggtagcgg ccggccccgt cggcctccac
       61 cgtggccgcg agcggcaacc ggagcgccgc ggcgcgcagg gcctcgccca ggccccggtc
      121 cgccagcagc gacgggtaga cccctcgtcc cagggcctgg atctcctcga gcgtcctgcc
      181 cagctcctcg ccgagggcgt ggagacgctc gagcccccgt tcgcggtcgt cccggatcag
      241 gtcctcggtc agctccagct ggatgcccag cgcgaccaaa cgttgctgtg cgccgtcgtg
      301 caggtcgcgc tcgatccggc gccgctcgcg gtcgccgctg gcctggatgc gcgtccgcga
      361 ccggcgcacc tcgtccaggg acgcctccac ctgggccgac aggcgccggt tctccagcgc
      421 ggtcaacgcg taggaggacg ccgcctcgag gaagtcggcc tcctccagga gcgcctcctc
      481 gtgcacgagc ccgacaacgg cgtgcccgtt ctggcgcacc tcggtgagac atcgcccccg
      541 gcccggcggc cccgaggggc gaccctgccc gtcaacccag cgcggcgggc cctcggcccg
      601 gtagaccagc tccagcgacg ggtcaccgac cgcggtcgag agcaccgccc gcagctgttc
      661 ccggtctgcc gccccccggg ccgcggtccc cagctgctgc agcgcgccgg cttcgtacag
      721 gcggcgctgg aggaggccga ccaggaacgc gacggccacc gcgggcacgg ccagcgccgc
      781 ggcccacacc acggcctcga ccagcgggga gtccggggcc gccctgcgga gcacgatgcc
      841 cagcgccagc agcccccagc gcgtggcggc caccaccagc acgggcagca gcgtcagccg
      901 cgtgagcggg ccggccccgc gcacccgctg ggccaccctg gccgtgaccg ccaggaacac
      961 gagcgtggtc agcgcctcgc gcagcggaac gaccacgtcg cccaagaagg ccggctcggt
     1021 gccgatctgg aacgcgttgc ccggacagcc accgtggcac gacgtgaact ggctcgggac
     1081 cgggaaggtc tccacgagca gcgtcgtggg cagatagagg accgcgaaga tcgcggccgc
     1141 cgcccccagc agcaggcggt cggcgcgcgc cgtcaggcgg cccgagggga aggccaggat
     1201 cagcccgatc agacccacct cgactgccca gccggcgacc cgcccgatgc tgtacagcag
     1261 ctcgtccgag gtctccgcga acgtcgtgag gaaccacccg aaaccggccg ccaccaggag
     1321 gcggccgaaa cggtcgtcgg ggcggctgtg ccaggcgtag aggccgaccc ctatgggcgc
     1381 cgccaccatc gaggcgcggg ccaggcccgc cagggccgca ccctcaccct gaacgctggt
     1441 cgacgtgatc acggccgcca cggccgtagc gcccagtccg gccacgccga tgaggacgta
     1501 ctcggccctg agccgccatc cggcggcagg ggcgctctct ggaagccggg cctggcgcac
     1561 tcgaactcct ccgtcggttc ggccgatcct ctcagcagcc ctgccgcggc gcaaccgccg
     1621 cacgagggcg ttcagggcga tctcgcgacc acgggctccg cgcgcgtcag ccgcaccatg
     1681 cggccggcgc tgagcagtcc caggaacccc gccaggatcg ggatcaggag cgcgacctgc
     1741 agggccaggt tcgtcgcgtc ggcgttgatg cccaggacct cctcgcgcac ggcctcgggc
     1801 tcgtccgcga gccgctcgtc caggtcgctg gtgctgatga cctgcgcgtc gtcctccagc
     1861 gtctgcgcga tctgccgctg ctgggaggag gggatctccg ggctggcgtt gctcatgttg
     1921 gtgaaggcca gcgagagggt ggccagcagg accccgccgg ccaccgccag cccgaaggag
     1981 aggccgaacg acccggacgc cgagttcagg ccggccgcct cgctgatccg ctcctcgt
//
LOCUS       DASUPW010000542         2660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_403079, whole genome shotgun sequence.
ACCESSION   DASUPW010000542 DASUPW010000000
VERSION     DASUPW010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403079"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VFY44_12235"
     CDS             <1..388
                     /locus_tag="VFY44_12235"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX5911253.1"
                     /translation="AYAGARHLILPCRTRERLADLDYDFDALKRLMLEHDLITVDLVW
                     RQDETTFHARNPFPVGGVVEDPATGAAAAAFGAYLRELGAVTPPVDLTILQGEDMGRP
                     SKLRVHVDTAERPELRVSGTAVTIAP"
     gene            385..675
                     /locus_tag="VFY44_12240"
     CDS             385..675
                     /locus_tag="VFY44_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911254.1"
                     /translation="MREPVRIVLRLLSLLLFVGGLALFLMIFVPGPGTVAEFMGESCS
                     HDGGTSSEQCTILDVAGISFGAAPVVMLVALIGLVALRKPGKGPVTLDLSRR"
     gene            complement(662..1258)
                     /locus_tag="VFY44_12245"
     CDS             complement(662..1258)
                     /locus_tag="VFY44_12245"
                     /inference="COORDINATES: protein motif:HMM:NF037271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX5911255.1"
                     /translation="MAVAYPTAARQLLRDTLLDATRELIAEREWGQVSMADVARTAGV
                     SRQTLYKEFGTRGEFAQAFVLREADRFLGEVETAVMANQKDPGTALTAALTVFLRAAT
                     DDPFVRTITTGVGADELLPLLTVQGQPLFVHATERLTAVLLEGWPGVRERDARQLADV
                     AVRLGVSYATAPSGPPERTAAAAGRVLEPFVRQALSGG"
     gene            complement(1258..1428)
                     /locus_tag="VFY44_12250"
     CDS             complement(1258..1428)
                     /locus_tag="VFY44_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEX5911256.1"
                     /translation="MSVFVCPECEYAFDEEKGDPREGFPPGTSWSEVPDDWACPDCGV
                     RDKVDFEPAGAA"
     gene            complement(1425..2615)
                     /locus_tag="VFY44_12255"
     CDS             complement(1425..2615)
                     /locus_tag="VFY44_12255"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HEX5911257.1"
                     /translation="MSDHAHHHHEHEAIEFNDVAWTDPKRYAWLLGLLVPVIPFIAWG
                     LVEATGLGVFWFFGPVFLYVIMPALDTAIGKDSANPPDSVLKRLEGDRYYRFCTYAYI
                     PLQFASLVGACWFWAYGDLSFVESLGLALTVGLVGGIGINTAHELGHKRPAMEKWLSK
                     VALAQTGYGHFFIEHNRGHHVRVATPEDPASSRLGESFWRFLPRTVWGSLTSAWHLES
                     ERLGRSGKSPFTLRNDILNAWAMTAVLFAGLTAVFGWVVLPWLVFQAVYGFSLLEVVN
                     YLEHYGLLRQKRDDGRYERTRPEHSWNSDNVSSNVLLYHLQRHSDHHAHPLRRFQALR
                     HFDEAPELPSGYGTMILIALFPPVWRRVMDHRVLEHYEGDVTKANILPARREKVIRKF
                     GVAA"
BASE COUNT          387 a          922 c          975 g          376 t
ORIGIN      
        1 ggcctacgcc ggggcgcggc acctgatcct gccctgccgg acccgcgagc gcctggccga
       61 cctggactac gacttcgacg cgctcaagcg gctgatgctg gagcacgacc tgatcaccgt
      121 ggacctggtc tggcgccagg acgagacgac cttccacgcg cgcaacccgt tcccggtcgg
      181 cggggtggtc gaggatcccg ccaccggcgc ggcagccgcg gccttcggcg cctacctgcg
      241 cgagctgggc gccgtgacgc caccggtgga cctgaccatc ctccagggcg aggacatggg
      301 ccggcccagc aagctgcggg tgcacgtgga cacggcggag cggcccgagc tgcgggtcag
      361 cggcacggcg gtgacgatcg cgccgtgagg gagccggtgc gcatcgtcct gcggctgctg
      421 agcctgctgc tgttcgtcgg tggcctggcc ctgttcctga tgatcttcgt gcccggcccg
      481 gggacggtgg ccgagttcat gggcgagtcg tgctcgcacg acggtgggac cagcagcgag
      541 caatgcacga tcctggacgt ggcggggatc tccttcggcg cggcgcccgt ggtcatgctg
      601 gtcgcgctga tcgggctggt cgcgctgcgc aagccgggca agggcccggt cacgctcgac
      661 ctcagccgcc gctgagggcc tggcgcacga acggctcgag cacccggccg gcggccgccg
      721 ccgtgcgctc gggggggccc gacggggcgg tcgcgtagct cacgcccagc cgcacggcca
      781 cgtcggccag ctggcgggcg tcgcgctcgc ggacccccgg ccagccctcc agcagcaccg
      841 cggtcaggcg ctcggtggcg tgcacgaaca gcggctggcc ctgcacggtc agcagcggca
      901 gcagctcgtc ggccccgacg ccggtggtga tcgtgcgcac gaacgggtcg tcggtcgcgg
      961 cgcgcaggaa cacggtcagc gcggcggtca gcgccgtgcc ggggtccttc tggttggcca
     1021 tgaccgcggt ctcgacctcg cccagaaaac ggtcggcctc gcgcagcacg aacgcctgcg
     1081 cgaactcccc gcgggtgccg aactccttgt agagggtctg gcggctgacg ccggccgtcc
     1141 gggccacgtc ggccatcgag acctggcccc actcgcgctc ggcgatcagc tcgcgggtgg
     1201 cgtccagcag cgtgtcgcgc agcagttgcc gcgccgcggt ggggtaggcg acggccatca
     1261 ggccgcgccg gcgggctcga agtccacctt gtcgcggacg ccgcagtccg ggcacgccca
     1321 gtcgtcgggg acctcgctcc acgacgtgcc cggcgggaag ccctcgcgcg ggtcgccctt
     1381 ctcctcgtcg aaggcgtact cgcactccgg gcacacgaag acgctcacgc ggccaccccg
     1441 aacttgcgga tcaccttctc ccgccgcgcc ggcaggatgt tggccttggt cacgtcgccc
     1501 tcgtagtgct cgagcacgcg gtggtccatg acccggcgcc acaccggcgg gaacagcgcg
     1561 atcaggatca tggtcccgta gcccgagggc agctcggggg cctcgtcgaa gtggcgcagc
     1621 gcctggaacc ggcgcagcgg atgggcgtgg tggtccgagt ggcgttgcag gtggtagagc
     1681 agcacgttgg aggacacgtt gtcgctgttc cagctgtgct cgggacgggt gcgctcgtag
     1741 cggccgtcgt cgcgcttctg gcgcagcagg ccgtagtgct cgaggtagtt cacgacctcg
     1801 agcagcgaga acccgtacac ggcctggaac accagccacg gcagcaccac ccagccgaac
     1861 accgccgtca acccggcgaa cagcaccgcg gtcatggccc atgcgttgag gatgtcgttg
     1921 cgcagcgtga acgggctctt gccggagcgg cccaggcgct cggactccag gtgccaggcg
     1981 gaggtcaggc tgccccagac ggtgcggggc aggaagcgcc agaagctctc accgaggcgt
     2041 gagctggccg ggtcctcggg cgtggccacg cgcacgtggt ggccgcggtt gtgctcgatg
     2101 aagaagtgcc cgtagccggt ctgggccagc gcgaccttgg acagccactt ctccatggcc
     2161 ggccgcttgt gccccagctc gtgggcggtg ttgatgccga tgccgccgac caggcccacg
     2221 gtcagggcca ggcccaggct ctcgacgaag ctcaggtcgc cgtaggccca gaaccagcac
     2281 gcgccgacca gcgaggcgaa ctgcagcggg atgtacgcgt aggtgcagaa gcggtagtag
     2341 cggtcgccct ccaggcgctt gagcacgctg tcgggcgggt tggccgagtc cttgccgatc
     2401 gccgtgtcca gcgcgggcat gatcacgtac aggaacaccg ggccgaagaa ccagaacacg
     2461 cccaggccgg tggcctcgac caggccccag gcgatgaagg ggatcacggg caccagcagg
     2521 cccagcagcc acgcgtagcg cttggggtcg gtccaggcca cgtcgttgaa ctcgatggcc
     2581 tcgtgctcgt gatggtgatg ggcgtggtcg ctcatggttg acaacggtac caccacttgt
     2641 caaccagagt tacacggagg
//
LOCUS       DASUPW010000543         3229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1787617, whole genome shotgun sequence.
ACCESSION   DASUPW010000543 DASUPW010000000
VERSION     DASUPW010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1787617"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VFY44_12260"
     CDS             complement(<1..906)
                     /locus_tag="VFY44_12260"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEX5911258.1"
                     /translation="MAREKDTSIPTGRIRRTAKVAGLAGGQTARNYATRAANLTRDEE
                     GRRAAATKRQAEAAEQILEVLGNMKGAAMKVGQVASFIDTGAFPEEFQDRIQAKLAEL
                     RDSAPQVSFKDMKKVIESELDEKLGDAFAEFDQDALAAASIGQVYKAKLHDGRTVAVK
                     VQYPGVAQAVRSDLQNLGMIMRVAKKIAPGMDAKAMTAEIKERLSDELDYEQEAQAQR
                     AFARAFRGHPFVVIPDVVTSLSREKVLVTEWVEGIGFEEVRELPQEKRDHYGEMIFRF
                     FFGSMHRNAHFSGDPHPGNFRFMPDG"
     gene            933..1751
                     /locus_tag="VFY44_12265"
     CDS             933..1751
                     /locus_tag="VFY44_12265"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX5911259.1"
                     /translation="MRFVTFDAGQGPQAGRLADGEVAPITAPGEGDLGELLRRGPLPS
                     TAPGVPVPLSEVRLLPPIPRPGKIICIGLNYAAHAAEQGVETPEYPTVFAKFANALAP
                     AGATVDLPSFSQKVDYEAEVAFVIGDTCKDVPEAEALEHVAGYTLLNDLSARDYQRIS
                     AQWMPGKVFDHAAPCGPALVTPDEAGPADAIGIWLTLNGQEMQNSSTADLIHSVPALV
                     AFLSKLMTLEPGDIVSTGTPSGVGMSRDPRVWLRPGDEVVVGSPQLGELRTTLA"
     gene            1791..>3229
                     /locus_tag="VFY44_12270"
     CDS             1791..>3229
                     /locus_tag="VFY44_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911260.1"
                     /translation="MIRQGIPLRRAVACAFAVLVALPAAAAAQEQGAHSDNMRFVKNI
                     PYEARNQTPNYGTDMEFARIGGRDYAVAGSEENGMRIIDVSRPEKAKVVGTYDCDILQ
                     GDVQVFRQKQRKGRVYATYTADADGNPDSACYREAEARGFDAVDGDRGRQGTFVVDIT
                     DPRRPRTASFVEVEQGSHNQTVHPSGNYLYNSNADLITSLQPAIEVIDISNLAAPHKT
                     GELALPTRPGLGTESHDISFDAKGDRAYSAALSQGVVIDSSKPARPTIVSSFVDPAVN
                     VWHQAEPVKVGSREFLIVEDEFAGAAGGPVCPSGGFHVYDITGDREKAPVKVGAWNID
                     EVRVTSSVTNTCTAHVFDVHPAQDLLTVAYYNGGVRVVDISSLAGIGLGKTDLVGAGM
                     REVGFYRHADADTWSAKTPRIAKDGSFYLYGNDIARGFDVYRFDGKGRTSKRDGRWLS
                     PAQARREALKLPDVKLPLGLAAGSAKAARR"
BASE COUNT          474 a         1151 c         1138 g          466 t
ORIGIN      
        1 gccgtcgggc atgaagcgga agttgcccgg gtgggggtcg cccgagaagt gcgcgttgcg
       61 gtgcatggag ccgaagaaga agcggaagat catctcgccg tagtggtcgc gcttctcctg
      121 gggcagctcg cgcacctcct cgaagccgat cccctcgacc cactcggtga ccaggacctt
      181 ctcgcgcgag agcgaggtca ccacgtccgg gatgaccacg aaggggtggc cccggaaggc
      241 gcgcgcgaac gcgcgctggg cctgggcctc ctgctcgtag tccagctcgt cggacaggcg
      301 ctccttgatc tcggcggtca tggccttggc gtccatgccc ggcgcgatct tcttggccac
      361 ccgcatgatc atccccaggt tctggaggtc cgagcgcacg gcctgggcga cgccggggta
      421 ctggaccttg acggccacgg tgcgcccgtc gtggagtttg gccttataga cctggccgat
      481 cgaggcggcg gccagcgcgt cctggtcgaa ctcggcgaag gcgtcgccga gcttctcgtc
      541 cagctcggac tcgatcacct tcttcatgtc cttgaacgag acctggggcg cggagtcgcg
      601 cagttcggcc agcttcgcct ggatccggtc ctggaactcc tcggggaagg cgccggtgtc
      661 gatgaacgac gccacctggc ccaccttcat ggccgcgccc ttcatgttgc ccagcacctc
      721 gaggatctgc tcggccgcct cggcctggcg cttggtggcc gccgcgcggc ggccctcctc
      781 gtcgcgcgtc aggttggcgg cgcgggtggc gtagttgcgc gccgtctgcc cgccggccag
      841 tcccgccacc ttggcggtcc ggcggatcct gccggtggga atggaggtgt ccttctcgcg
      901 agccacgctc ctagggtacg gtcccgcgcg caatgcggtt cgtgaccttc gacgcggggc
      961 agggccccca ggccggccgg ctggccgatg gcgaggtcgc gcccatcacc gcgccgggcg
     1021 agggcgacct gggcgagctg ctccggcgcg gccctctgcc ctcgaccgcg cccggcgtcc
     1081 cggtgccgct ctccgaggtc aggctgctgc cgccgatccc gcggccgggg aagatcatct
     1141 gcatcgggct gaactacgcc gcgcacgcgg ccgagcaggg cgtcgagacg cccgagtacc
     1201 ccacggtgtt cgccaagttc gccaacgcgc tggcgccggc cggcgcgacc gtggacctgc
     1261 cctcgttcag ccagaaggtg gactacgagg ccgaggtggc gttcgtgatc ggcgacacgt
     1321 gcaaggacgt gcccgaggcc gaggcgctgg agcacgtggc gggctacacg ctgctcaacg
     1381 acctgtcggc gcgcgactac cagcgcatca gcgcccagtg gatgccgggc aaggtgttcg
     1441 accacgcggc gccttgcggc ccggcgctgg tgaccccgga cgaggcgggc ccggccgacg
     1501 cgatcggcat ctggctcacg ctgaacggcc aggagatgca gaacagctcg accgcggacc
     1561 tgatccactc ggtgcccgcg ctggtggcgt tcctctcgaa gctgatgacg ctcgagccgg
     1621 gtgacatcgt ctccaccggc acgccctcgg gggtgggcat gtcgcgcgac ccgcgcgtgt
     1681 ggctgaggcc cggcgacgag gtcgtggtcg gctcgcccca gctcggcgag ctgcgcacca
     1741 cgctggccta gcccgtttga ccttcccccg gacgggggat aggtgcgctc gtgatccggc
     1801 aggggatccc tctacgtcgt gccgtcgcgt gcgcgttcgc cgtgctcgtc gcgctgcccg
     1861 cggccgcggc cgcccaggag cagggcgccc acagcgacaa catgcgcttc gtcaagaaca
     1921 tcccgtacga ggcacgcaac cagacgccca actacggcac cgacatggag ttcgcccgga
     1981 tcggcggccg cgactacgcg gtggccggct cggaggagaa cgggatgcgc atcatcgacg
     2041 tctcgcggcc cgagaaggcc aaggtcgtgg gcacctacga ctgcgacatc ctccagggcg
     2101 acgtccaggt gttccgccag aagcagcgca aggggcgcgt ctacgcgacc tacaccgcgg
     2161 acgccgacgg caaccccgac tcggcctgtt accgggaggc cgaggcccgg ggattcgacg
     2221 cggtggacgg cgaccgcggc cgccagggca cgttcgtggt ggacatcacc gacccgcgcc
     2281 gcccgcggac cgcgtcgttc gtcgaggtcg agcagggatc gcacaaccag accgtgcacc
     2341 ccagcggcaa ctacctctac aactcgaacg ccgacctgat cacgtcgctg cagcccgcca
     2401 tcgaggtcat cgacatctcg aacctggcgg cgccgcacaa gacgggtgag ctggccctgc
     2461 ccacgcgccc cggcctgggc accgagtcgc acgacatctc cttcgacgcc aagggcgacc
     2521 gcgcgtactc ggcggcgctc tcgcagggcg tcgtcatcga cagctccaag cccgccaggc
     2581 ccacgatcgt ctcctcgttc gtcgacccgg ccgtgaacgt ctggcaccag gccgagccgg
     2641 tcaaggtggg cagccgcgag ttcctgatcg tcgaggacga gttcgcgggc gccgccggcg
     2701 ggccggtgtg ccccagcggc ggcttccacg tgtacgacat caccggcgac cgcgagaagg
     2761 cgccggtgaa ggtcggggcc tggaacatcg acgaggtgcg cgtgacctcc agcgtgacca
     2821 acacctgcac ggcgcacgtc ttcgacgtcc atccggccca ggacctcctg accgttgcct
     2881 actacaacgg cggcgtccgg gtggtggaca tctccagcct ggccgggatc ggcctgggca
     2941 agaccgacct ggtgggcgcc gggatgcgcg aggtgggctt ctaccgccac gcggacgccg
     3001 acacgtggtc ggccaagacg ccccggatcg ccaaggacgg gagcttctac ctgtacggca
     3061 acgacatcgc ccgcgggttc gacgtctacc gcttcgacgg caagggcagg acgtccaagc
     3121 gcgacgggcg ctggctcagc cccgcgcagg cgcgacgtga ggcgctgaag ctgccggatg
     3181 tgaagctgcc gctgggcctg gccgccggga gcgcgaaggc ggcccggcg
//
LOCUS       DASUPW010000544         2117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_955773, whole genome shotgun sequence.
ACCESSION   DASUPW010000544 DASUPW010000000
VERSION     DASUPW010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955773"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VFY44_12275"
     CDS             complement(<1..515)
                     /locus_tag="VFY44_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911261.1"
                     /translation="MSERTQCPPERRVAEVKGVRRLGAYDLITARDPIGPENPQPGQF
                     YMLAAGDGWGGGSGERPFLPRAFSHAGARRHMGGGVELDFLLEDVGPGTRRLGQMDAG
                     EPLMLLGPLGGGFRPAAEGTRPLLVGGGIGVAPLLCLYADLLAAREAPQVILGFRGEQ
                     HAAGAELFAGAA"
     gene            complement(512..>2117)
                     /gene="carB"
                     /locus_tag="VFY44_12280"
     CDS             complement(512..>2117)
                     /gene="carB"
                     /locus_tag="VFY44_12280"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX5911262.1"
                     /translation="HGPRHEVERGDRPSVMILGSGPNRIGQGIEFDYCCVHAAMTVRA
                     SGRDAVMVNCNPETVSTDYDTSDRLYFEPLTLEDVLGVIELEQPEGVIVQFGGQTPLK
                     LAQGLVDAGVPLLGTPVESIHLAEDRPSFGAILDELGLKSPPYATAGSPEQALSAAEE
                     VGYPLLVRPSYVLGGRAMEICYSRESLDGYLQRTAEGGRPEGDIFLDRFLENAIEVDV
                     DALCDGETVRIGAVMQHVEEAGIHSGDSACVIPAMSLGPDMHRQVEDAAARIALRLGV
                     VGLINIQFAVYGDDELYVIEANPRASRTVPFVSKAVGVPLAKVAARLMLGERLADMEL
                     ALPSSGSHVSVKEAVLPFGRFPRADSLLGPEMKSTGEVMGVAQDYPTAFGKAQAAAGA
                     ILPSEGTVFISVTDGDKPTATQLAASLHDLGFTIMATGGTAQAIRRMGVPVERIQKLS
                     EGSPNVVEAIDAGQVDLVVNTPTGSGARADGYEIRRAAVARGVPCITTMSGASAAQRA
                     IRAGRGEAPPVTSLQDLHSAARQVPT"
BASE COUNT          278 a          793 c          768 g          278 t
ORIGIN      
        1 gcggctcccg cgaacagctc ggcccctgcc gcgtgctgct cgccgcggaa gcccaggatc
       61 acctgcggag cctcgcgcgc ggccagcagg tcggcgtaca ggcacagcaa gggggcgacg
      121 ccgatgccgc cgccaaccag cagcggccgc gtgccctcgg ccgcggggcg gaagccgccg
      181 ccgagcgggc ccagcagcat cagcggctcg cccgcgtcca tctggcccag ccggcgcgtg
      241 cccgggccca cgtcctcgag caggaagtcc agctccaccc cgccgcccat gtggcggcgc
      301 gcccccgcgt gcgagaacgc ccggggcagg aacgggcgct cgccgctgcc cccaccccac
      361 ccgtcgcccg cggccagcat gtagaactgg cccggttgcg ggttctccgg gccgatcggg
      421 tcgcgggcgg tgatcaggtc gtaggcgccc aggcggcgca cgcccttgac ctcggccacc
      481 cgccgctcgg gcgggcactg ggtgcgttcg ctcacgtggg gacctgcctg gcggccgagt
      541 gcaagtcctg cagcgacgtg acgggcggcg cctccccacg cccggcgcgg atggcccgct
      601 gggcggcgct ggcgccgctc atggtggtga tgcagggcac gccgcgtgcg accgccgcgc
      661 gccggatctc gtacccgtcc gcgcgggcgc cggagcccgt gggcgtgttc accaccaggt
      721 ccacctggcc ggcgtcgatg gcctcgacca cgttgggcga cccctcgctg agcttctgga
      781 tccgctccac ggggactccc atgcggcgga tggcctgcgc cgtcccgccc gtggccatga
      841 tcgtgaagcc caggtcgtgc agggacgccg cgagctgggt ggcggtgggc ttgtcgccgt
      901 cggtcacgct gatgaagacc gtgccctccg acggcaggat ggccccggcg gcggcctggg
      961 ccttgccgaa cgcggtcggg tagtcctggg ccacgcccat gacctcgccg gtggacttca
     1021 tctcggggcc cagcagcgag tcggcgcgtg ggaagcgccc gaacggcagc accgcctcct
     1081 tgaccgagac gtggctaccg ctggagggca gcgcgagctc catgtcggcc agccgctcgc
     1141 ccagcatcag gcgggcggcc accttggcca gcggcacgcc cacggccttg gagacgaacg
     1201 gcacggtgcg cgacgcgcgc gggttggcct cgatcacgta cagctcgtcg tcgccgtaga
     1261 cggcgaactg gatgttgatc agtcccacca cccccagccg gagcgcgatc cgcgcggcgg
     1321 cgtcctcgac ctgacggtgc atgtcgggtc ccagcgacat ggccgggatg acgcaggccg
     1381 agtcgccgga gtggatgccg gcctcctcga cgtgctgcat cacggcgccg atgcgcacgg
     1441 tctcgccgtc gcagagggcg tccacgtcga cctcgatcgc gttctccagg aaccggtcca
     1501 ggaagatgtc gccctcgggc cggccgccct cggcggtgcg ctgcaggtag ccgtccagcg
     1561 actcgcggga gtaacagatc tccatcgccc gcccgcccag cacataggag gggcgcacca
     1621 gcagcgggta gcccacctcc tcggcggcgg agagcgcctg ctcgggcgag ccggccgtgg
     1681 cgtagggcgg gctcttcagg cccagctcgt ccaggatggc gccgaagctg gggcggtcct
     1741 cggccaggtg gatcgactcg accggcgtgc ccagcagcgg gacgccggcg tcgaccagcc
     1801 cctgggccag cttcagcggc gtctggccgc cgaactggac gatcacgccc tcgggctgct
     1861 ccagctcgat caccccgagc acgtcctcca gcgtcagcgg ctcgaagtac agccggtcgg
     1921 aggtgtcgta gtcggtcgag accgtctccg ggttgcagtt gaccatgacg gcatcgcggc
     1981 cggaggcgcg cacggtcatg gcggcgtgca cgcagcagta gtcgaactca atcccctgcc
     2041 cgatccggtt cggccccgag ccgaggatca tcacgctcgg ccggtcgccg cgctcgacct
     2101 cgtggcgcgg gccgtgg
//
LOCUS       DASUPW010000545         4088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_818003, whole genome shotgun sequence.
ACCESSION   DASUPW010000545 DASUPW010000000
VERSION     DASUPW010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818003"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="VFY44_12285"
     CDS             <1..946
                     /locus_tag="VFY44_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911263.1"
                     /translation="APRGRRECPRAARALRGVRWLALSPDGRNLYASSPANDAIAAFA
                     RNPRTGGLTQLPGGDACIEDRLARRGSGCPTGVGLNYPRTIAISPDGLNAYVASDTAD
                     SVIPGDPADGDAVSAFARDPRTGALRQLPGKAACIKDTIARSVTACETIGRGLLDAFN
                     VSVSPDGKHVYVASDASRLGAIAIFRREATGELTQLPGTSGCLGNGRGCGRAEGIAGT
                     DSVTLSPNGRRLYATGFFGWSLGVFSRDPATGAIRQLRGAKACVRDVNSKARCGQTIK
                     GLQGPRDVVLSPDGHTAYVPSSVAGTINVFAVGARPRR"
     gene            complement(909..1889)
                     /locus_tag="VFY44_12290"
     CDS             complement(909..1889)
                     /locus_tag="VFY44_12290"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5911264.1"
                     /translation="MTPAPPRVSIILPTYNGEGLLPGALASIRDQTYADWEAIVVDDG
                     SDDDTAAVAEAFAASDPERVTVVRLEHNVGVGGARTEGIARSRGGELLCLLDQDDLWD
                     ERYLECSVAAYDEAVAQGRPVAIISSNARIMGEDGLTDETWWGRNGWVTPVRLENMLR
                     RNYLNARSLFTRQAYERVGGDFARGLGGSDDLDLWLRILEQGYEAIGVREPLAIYRDH
                     AGSFSRHRLAMSEGKIACLRRALDRGHLGPRERRIARAQIRHWRALREAELCRRAVAA
                     RRPAGVLGHGARAVPLGAIAFAQDPARWREWGARVAARVSGAAGRRRRTR"
     gene            complement(1886..2968)
                     /locus_tag="VFY44_12295"
     CDS             complement(1886..2968)
                     /locus_tag="VFY44_12295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5911265.1"
                     /translation="MSAGPARERRVRVVHLGPSAAGRGGMGLSIQGLLRSPLAERYEM
                     VAIPTWRGTAGRVARVVIFLRALVSFLGWCLRRGPGIVHVHAGTRGSWYRKGVCVAVA
                     KLMRRPVLLQLRVGPWDIIEFDERIGPVRRAAARQLFARPDRVVSVSSAGADLIRTRY
                     GRAGVPVISNPAPEPRLDPVREDGPPTLLYVGGFHDPAKAGDVFLEALPRILEACPGA
                     RVVMAGPGELPGTGRRLVEERPEVTWLGWLDEQAKADAFAATEVFVLPSRSEGMPNAM
                     LEAMANGRAVVAAEVGGVPDIVTDHEDGLLVPAGDAEALAGAVSELLADGALRARLGT
                     AARSRAERLTRDEVWGRLDAVYRELA"
     gene            complement(2965..3918)
                     /locus_tag="VFY44_12300"
     CDS             complement(2965..3918)
                     /locus_tag="VFY44_12300"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5911266.1"
                     /translation="MRALVTGGGGFIGSNIVERLLERGDDVRVLDNFSTGKRENLAPF
                     VDDVELVEGDLRSYERVHNAVKDCEVVFHQGAMPSVPRSVQDPITSSDINVGGTLNVL
                     LAARDADARRVVFASSSSIYGDLPGLPRVETMPVGPAAPYAVSKLAAEHYCHVATKVY
                     GLTTVSLRYFNIFGKRQDPLSQYSAVIPKFIAALREGRSPTIHGDGEQSRDFTHIDNA
                     VAANLLAAEAPDAGGKCFNVATGESQSLNDLVRQLNELMGTDIPAEHGPQRPGDVAHS
                     LADISRAREVLGYEPAVSFQEGLRMTIESFDEGHKAAGLAR"
BASE COUNT          545 a         1584 c         1462 g          497 t
ORIGIN      
        1 ggcgccgcgc ggccggcggg agtgcccgcg cgcggccagg gcactgcgtg gcgtgcgctg
       61 gctcgccctg agcccggacg gccgcaacct ctacgcgtcg tcgcctgcaa atgacgccat
      121 cgccgcgttc gcccgcaacc cccgcaccgg gggcctgacc cagctgcccg gaggcgacgc
      181 ctgcatcgag gaccgcctgg cgcgccgggg cagcggctgc ccgacgggcg tcgggctgaa
      241 ctacccgcgg acgatcgcca tcagccccga cgggctgaac gcctacgtcg cctccgacac
      301 cgcggacagc gtcatccccg gcgacccggc cgacggcgac gcggtctccg cgttcgcccg
      361 cgatccccgc acgggggcgc tgcgccagct gcccggcaag gccgcctgca tcaaggacac
      421 gatcgcgcgc agcgtcaccg cgtgcgagac catcggccgc ggcctgctcg atgcgttcaa
      481 cgtcagcgtc agccccgacg gcaagcacgt ctacgtggcc tccgacgcca gccggctggg
      541 cgcgatcgcg atcttccggc gcgaggccac cggggagctg acccagctcc cggggacctc
      601 cggctgcctg ggcaacggca ggggctgcgg gcgggccgag ggcatcgccg gcaccgacag
      661 cgtcacgctc agccccaacg ggaggcggct ctacgccacc ggcttcttcg gctggtcgct
      721 cggggtgttc agccgcgacc cggcgacggg ggcgatccgc cagctgcggg gcgccaaggc
      781 ctgcgtccgt gacgtgaact ccaaggcccg ctgcgggcag acgatcaagg gcctccaggg
      841 ccccagggac gtggtgctca gcccggacgg gcacacggcg tacgtgccct ccagcgtggc
      901 cggcacgatc aacgtgttcg ccgtcggcgc ccggccgcgc cgctgaccct cgcggccacc
      961 cgcgcccccc actcccgcca gcgggcgggg tcctgggcga acgcgatcgc tcccaggggc
     1021 acggcccgcg ccccgtggcc cagcacgccg gccgggcgcc gcgccgccac ggcccggcgg
     1081 cacagctccg cctcgcgcag ggcgcgccag tggcggatct gggcacgcgc gatccgtcgc
     1141 tcacgcggcc ccaggtggcc ccggtcgagc gcccggcgca ggcacgcgat cttgccctcg
     1201 ctcatggcca ggcggtggcg cgagaacgag ccggcgtggt cgcggtagat ggccagcggc
     1261 tcgcggacgc cgatggcctc gtagccctgc tcgaggatcc gcagccacag gtccaggtcg
     1321 tccgaccccc cgaggccgcg ggcgaagtcg ccccccaccc gctcgtaggc ctggcgcgtg
     1381 aacagggagc gcgcattcag gtagttgcgc cgcagcatgt tctccagccg cacgggcgtg
     1441 acccacccgt tgcggcccca ccacgtctcg tcggtgagcc cgtcctcgcc catgatgcgc
     1501 gcgttggagg agatgatcgc caccggccgc ccctgggcga ccgcctcgtc gtaggcggcg
     1561 accgagcact cgaggtaccg ctcgtcccag aggtcgtcct ggtccagcag gcagagcagc
     1621 tctcccccac gggagcgggc gatcccctcg gtgcgggccc cgcccacccc cacgttgtgc
     1681 tccagccgga cgaccgtgac ccgctccggg tccgacgccg cgaacgcctc ggccaccgcg
     1741 gcggtgtcgt cgtcggagcc gtcgtccacc acgatcgcct cccagtccgc gtacgtctgg
     1801 tcgcggatcg aggccagcgc gccgggaagc aggccctcgc cgttgtaggt gggcaggatg
     1861 atcgacacgc gcggcggcgc cggcgtcacg ccagctcccg gtagacggcg tccagacgcc
     1921 cccacacctc gtcacgcgtc agccgctcgg cccgggaccg cgcggcggtg cccaggcggg
     1981 cgcgcagcgc gccgtcggcc agcagctccg agaccgcgcc ggccagcgcc tcggcgtcgc
     2041 ccgcgggcac cagcaggccg tcctcgtggt cggtgacgat gtcgggaacc ccgcccacct
     2101 ccgcggccac caccgcccgc ccgttggcca tcgcctcgag catggcgttg ggcatgccct
     2161 ccgaccgcga gggcagcacg aacacctcag tcgccgcgaa cgcgtccgcc ttggcctgtt
     2221 cgtccagcca tccgagccag gtcacctccg ggcgctcctc gaccagccgg cgcccggtgc
     2281 cgggaagctc gcccgggccg gccatgacca cccgggcgcc cgggcacgcc tcgaggatcc
     2341 gcggcagggc ttccaggaac acgtcgccgg ccttggcggg gtcgtggaag ccgcccacgt
     2401 agagcaacgt gggcgggccg tcctcgcgga cggggtccag ccggggctcg ggcgcggggt
     2461 tggagatcac ggggacgccg gcccgcccgt accgggtccg gatgagatcc gcgccggccg
     2521 aggacacgct gaccacgcgg tcgggccgcg cgaacagctg ccgggcggcc gcccggcgga
     2581 cggggcctat gcgctcgtcg aactcgatga tgtcccacgg gccgacgcgc agctgcagca
     2641 gcacggggcg gcgcatgagc ttggccacgg ccacgcagac ccccttgcgg taccagctcc
     2701 cgcgcgtgcc ggcgtggacg tgcacgatgc ccgggccccg gcgcaggcac cagcccagga
     2761 acgagaccag cgcgcggagg aagatcacga cccgggccac gcgcccggcg gtgcccctcc
     2821 aggtggggat ggccaccatc tcgtaccgct ccgccagcgg cgatcgcagc aggccctgga
     2881 tgctcagccc catcccgccc cggcccgcgg ccgacgggcc gaggtgcacc acacgcacgc
     2941 gccgctcgcg cgcggggccg gcgctcaccg cgccagtccg gccgccttgt ggccctcgtc
     3001 gaaggactcg atggtcatcc gcaggccctc ctggaagctc acggccggct cgtagcccag
     3061 cacctcgcgg gcccgcgaga tgtcggccag cgagtgggcc acgtcgcccg ggcgctgggg
     3121 gccgtgctcg gccgggatgt cggtgcccat cagctcgttc agctggcgga ccaggtcgtt
     3181 gagcgactgg gactccccgg tggccacgtt gaagcacttg ccgccggcat ccggggcctc
     3241 ggccgccagg aggttggccg ccacggcgtt gtcgatgtgg gtgaagtcac gcgactgctc
     3301 gccgtcgccg tggatggtcg gcgaccggcc ctcgcgcagc gccgcgatga acttggggat
     3361 caccgcgctg tactgcgaca gggggtcctg ccgcttgccg aagatgttga agtagcgcag
     3421 ggacaccgtg gtcaggccgt agaccttggt ggccacgtgg cagtagtgct cggcggcgag
     3481 cttggacacc gcgtacgggg cggccggccc cacgggcatc gtctccaccc gcggcaggcc
     3541 gggcaggtcg ccgtagatgg acgaggagga cgcgaagacc acccggcgcg cgtcggcgtc
     3601 gcgggcggcc aggaggacgt tgagcgtccc ccccacgttg atgtcgctgc tggtgatcgg
     3661 gtcctggacc gaccggggca ccgacggcat ggcgccctgg tggaagacca cctcgcagtc
     3721 cttgaccgcg ttgtggacgc gctcgtagct gcgcaggtcg ccctccacga gctcgacgtc
     3781 gtcgacgaac ggcgccaggt tctcgcgctt gccggtcgag aagttgtcca gcacccggac
     3841 gtcgtcaccc cgctccaaca gccgctcgac gatgttggag ccgatgaaac cgccgccccc
     3901 cgtcaccagc gcgcgcatga gccatacctc cttgtcggac tcccgcaggc caaaccaggg
     3961 ccaacgtaac acgggtcccc cggcctcgct cggcgacgat gtcgtgagct acgggtcatc
     4021 gggagccgat caccccgagc gccttgcggg cggcgctcgc cagcgtgcgg gccagcttcg
     4081 tggggccg
//
LOCUS       DASUPW010000546         5370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_125183, whole genome shotgun sequence.
ACCESSION   DASUPW010000546 DASUPW010000000
VERSION     DASUPW010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125183"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VFY44_12305"
     CDS             complement(<1..409)
                     /locus_tag="VFY44_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911267.1"
                     /translation="MTDDSPMKGALDVPYREREKSVDIGSLPAPDMPIAGTELEPETE
                     LLTINIGPHHPATHGVLRLLCTLEGETVLDLEPIVGYVHTGIEKSCEDQEYWKVIPFV
                     ERMDYLSYYFNAMAYCNSVETLLGEEVPPRAQYL"
     gene            complement(402..905)
                     /locus_tag="VFY44_12310"
     CDS             complement(402..905)
                     /locus_tag="VFY44_12310"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX5911268.1"
                     /translation="MELVAQRVKDAVGDDAVVDTLHAHGQATLQVAPESVHDVLAHLR
                     DADDEPYEMLASVHGCDYLPEEPRLGVHYQLLSMERAERLNVKTRLGVEEPRLATVTD
                     LYPTAGYQEREIYDFFGVEFEGHPDLRRILMPEDYVGHPQRRDFPMGGEPILFTFNED
                     ELPRWYD"
     gene            complement(916..1572)
                     /locus_tag="VFY44_12315"
     CDS             complement(916..1572)
                     /locus_tag="VFY44_12315"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX5911269.1"
                     /translation="MTRTKVPGSKEEFRSRQLKAQQMLRGDLEGSDLDEYVEKSVLLT
                     RFEDAQNWARKNAIFPLGFGLACCAMEMISVIGSPRNDLARFGAEAIRFTPRQADLLI
                     LSGRVSIKMAPVIRRIYEQMLEPKWVISMGACASSAGMFNNYALVAGADKFLPIDVYV
                     PGCPPRPEALIYGIMQLQDKIQGSPHQGWRERYRSAGTEETGTSIAAEGKGEGEAPDA
                     "
     gene            complement(1569..1925)
                     /gene="ndhC"
                     /locus_tag="VFY44_12320"
     CDS             complement(1569..1925)
                     /gene="ndhC"
                     /locus_tag="VFY44_12320"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX5911270.1"
                     /translation="MLKSYLPALVFLLLGVGIGVAFTLANNALGPSKPGKVKSEPYES
                     GLPSDVRKSFRFGLSFYMIAMLFILFDIEVILIYPVALGLQEFGVAALVKIIIFVALL
                     AVAFAHQWRRGALDWK"
     gene            1995..2426
                     /locus_tag="VFY44_12325"
     CDS             1995..2426
                     /locus_tag="VFY44_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911271.1"
                     /translation="MSQVHMIVGISVLASNAVAAAWGGVSWLRGFPSHPFWWLLRVAQ
                     ITVVVQVVIGLLLLARGSSAPDGLHVAYGVALLAVTVIAEGTRLGAAQRLLEDVPDLD
                     ALGREEQIALARRVSLAEMGVMTIGVLLVLTLSLRAYQTGG"
     gene            2510..2917
                     /locus_tag="VFY44_12330"
     CDS             2510..2917
                     /locus_tag="VFY44_12330"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5911272.1"
                     /translation="MSTSSDQRKRKLEIPAELDRLRETREWANQVAREHGLSEDDCFQ
                     VKLAMSEAVTNAIIHGSSGQADMVRIVACMGEEQLVFEVTDPGRLDTGDPVERLDEGG
                     RGLELVSMVMDEVQLVRGERGGSLRFAKRFAAA"
     gene            3116..3517
                     /locus_tag="VFY44_12335"
     CDS             3116..3517
                     /locus_tag="VFY44_12335"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5911273.1"
                     /translation="MTRSDTTRGAAVLACVGALAVLGSGCGGEVKADNETDRAAAVSF
                     NQHCSGCHTLKPANSYGSKPQGDIKPGERTNGPNFNQRKVSRDDALYAIRNGGFSGAI
                     MPANIVTGREADRLADYLAKYSGRERTSDAN"
     gene            complement(3514..>5370)
                     /locus_tag="VFY44_12340"
     CDS             complement(3514..>5370)
                     /locus_tag="VFY44_12340"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5911274.1"
                     /translation="AKKTVPGSRVAGFGSTGDRTFVSEDGRTAFVYAFTPLSDDPFGA
                     NTEVMRELRSALAGAEVAGAPVRLTGYDALFDSSGEGDDGPGVLLEAVLGGVGALVVL
                     VFVFGSALAFVPLAMAVCSILVSVLLLYALTAITEVSPIVQFLVALVGLGISIDYALL
                     VVVRWREELERGREGDDAVAAAMSTAGRAVVFSGTTVAVGLLALIALPLPFLRSMGYG
                     GMIIPLVATLAAITLLPVVLATIGPRLDRRRIRRSDRSARFWQGWAEGIVRHRGVAAV
                     IAVAILVALLIPATDLHLGNSDPDTIAKAGPAKEGLHDLRDSGIGTGAIAPFETLAPV
                     ADADAVVKAQQGVDGMHGAVAPQNPAWRKNGEALIAGVPNEGDETAEGRAAVDEVRDA
                     SHAVGPDVRVGGSGPLNADFIDAVYGAFPLMIGLISLLTFLLLARAFRSLLLPLKAVI
                     LNILSVGAAWGVMALIWQNGHGSDEIWGIAATGSIASWIPLMVFAFLYGLSMDYEVFI
                     LSRMREEFDKGGDTDKAVIEGLARTGRLVTSAALILFLAFASMASGPETDVKILATGL
                     AAGILLDATVVRALLVPAVVSMFGRWNWWLPPGPAKLLRVKPSPEPATEPAA"
BASE COUNT          823 a         1890 c         1885 g          772 t
ORIGIN      
        1 gcaggtactg cgcgcggggc gggacctcct cgccgaggag cgtctcgacc gagttgcagt
       61 acgccatcgc gttgaagtag taggacaggt agtccatccg ctccacgaac gggatgacct
      121 tccagtactc ctggtcctcg cagctcttct cgatgcccgt gtgcacgtag cccacgatcg
      181 gctcgaggtc caggaccgtc tcgccctcca gggtgcacag cagccgcagc acgccgtggg
      241 tggccgggtg gtgcggtccg atgttgatgg tcaggagctc ggtctcgggc tccagctcgg
      301 tgccggcgat gggcatgtcc ggcgccggca ggctgccgat gtccacggac ttctcgcgct
      361 cgcggtacgg gacgtccagc gcgcccttca tgggcgagtc gtcagtcata ccaacgcggc
      421 agttcgtctt cgttgaacgt gaagaggatc ggctcgccgc ccatcgggaa gtcgcgccgc
      481 tgggggtggc ccacgtagtc ctcgggcatc aggatccggc gcaggtccgg gtggccctcg
      541 aactccacgc cgaagaagtc gtagatctcg cgctcctggt agccggccgt ggggtagagg
      601 tcggtcaccg tggccagcct cggctcctcg acccccagcc gggtcttcac gttcaggcgc
      661 tcggcccgct ccatgctcag cagctggtag tgcacgccca gccgcggctc ctccggcagg
      721 tagtcgcagc cgtggacgct ggccagcatc tcgtagggct cgtcgtcggc gtcgcgcagg
      781 tgggccagca cgtcgtgcac cgactccggg gccacctgga gggtggcctg gccgtgggcg
      841 tgcagggtgt ccacgacggc gtcgtcgccg acggcgtcct tcacgcgttg ggcgaccagt
      901 tccaaccctg ttgcgtcagg catcgggggc ctcgccctcc cctttgcctt cggccgcgat
      961 gctggtgccc gtctcctcgg tgccggccga gcggtagcgc tcgcgccagc cctggtgggg
     1021 gctgccctgg atcttgtcct gcagctgcat gatcccgtag atcagggcct cgggccgggg
     1081 cgggcagccg ggcacgtaga cgtcgatcgg caggaacttg tcggcccccg cgaccagcgc
     1141 gtagttgttg aacatgccgg cgctcgaggc gcacgcgccc atggagatga cccacttggg
     1201 ctcgagcatc tgctcgtaga tgcgccggat gaccggcgcc atcttgatcg acacccggcc
     1261 ggacaggatc agcaggtcgg cctggcgcgg ggtgaagcgg atggcctcgg cgccgaagcg
     1321 ggccaggtcg ttgcgcgggg agccgatcac gctgatcatc tccatcgcgc agcaggccag
     1381 gccgaagccc agcgggaaga tcgcgttctt gcgcgcccag ttctgggcgt cctcgaaccg
     1441 ggtcagcagg accgacttct cgacgtactc gtccaggtcc gacccctcga ggtcgccgcg
     1501 cagcatctgc tgggccttga gctggcgcga gcggaactcc tccttggagc cgggcacctt
     1561 cgtgcgggtc acttccaatc caatgcaccc cttctccact ggtgggcgaa cgccactgcc
     1621 agcagcgcca cgaagatgat gatcttgacc agcgccgcga cgccgaactc ctggagcccc
     1681 agcgccaccg ggtagatcag gatgacctcg atgtcgaaca ggatgaacag catcgcgatc
     1741 atgtagaagc tgagcccgaa gcggaagctc ttgcgtacgt cggagggaag cccggactcg
     1801 tagggctcgc tcttgacctt gccgggcttg ctcggcccga gggcgttgtt ggccagcgtg
     1861 aacgcgacgc cgatgcccac gcccagcagc aagaagacaa gtgctggcag atagctcttc
     1921 agcaaggtgt cgcatgtata acgcaggctt gtgcggcatt gctacatagt gcgaaagctt
     1981 gctaggagcc ggatttgagc caggtgcaca tgatcgtggg catctcggtg ctggccagca
     2041 acgccgtggc ggcggcctgg ggcggcgtgt cgtggctgcg cgggttcccc tcgcatccgt
     2101 tctggtggct cctgcgcgtg gcccagatca ccgtggtggt ccaggtggtc atcgggctgc
     2161 ttctcctcgc gcgcgggtcc agcgccccgg acgggctgca cgtggcctac ggcgtggcgc
     2221 tgctggcggt cacggtcatc gcggagggca cccggctggg ggccgcacag cgcctgctcg
     2281 aggacgtgcc cgacctggac gcgctgggcc gcgaggagca gatcgcgctg gcccggcgcg
     2341 tgtcgctggc cgagatgggg gtcatgacca tcggggtgct gctggtgctg accctgtcgc
     2401 tgcgcgccta ccagaccgga gggtgaacgc cggccgggag gttggaaacg cggcgtaccg
     2461 gggataggca gagggtcggg caggcaaaga agggcaggca aacgcgttga tgagcaccag
     2521 cagcgaccag cgaaagcgaa agctggagat ccccgctgag ctggacaggc tccgcgagac
     2581 ccgcgagtgg gccaatcagg tggcccgcga gcacgggctc tccgaggacg actgtttcca
     2641 ggtcaagctc gcgatgagcg aggcggtgac caacgccatc atccacggct ccagcggcca
     2701 ggcggacatg gtgcgcatcg tggcctgcat gggcgaggag cagctcgtgt tcgaggtcac
     2761 cgacccgggc cggctggaca ccggcgaccc ggtcgagcgc ctggacgagg gcgggcgcgg
     2821 cctggagctg gtctccatgg tcatggacga ggtccagctg gtgcgcggcg agcggggcgg
     2881 ctcgctgcgc ttcgcgaagc gcttcgccgc ggcctgaggt cacgtcttgc ggccgtccat
     2941 ggccgcgctt ctgatgcgaa ctccgccgtc ccggcggaga gagccccggc gccggcgctg
     3001 tatcgccgcc gatgtgggcg atcccggtac tgccggaccg ccgcggccgc cgccggggca
     3061 cctgttgatt cgtcctccgg ggcccggatc ctctcgctca gtaatatgcc cgcgcgtgac
     3121 ccgctccgac acaacccgcg gcgccgccgt gctcgcgtgc gtgggggcgc tggccgtgct
     3181 cgggtccgga tgtggcggtg aggtcaaagc cgacaacgag accgaccgcg cggcggcggt
     3241 gtcgttcaac cagcactgct cgggctgtca cacgctcaag cccgccaact cctacggctc
     3301 caagccgcag ggcgacatca agcccggcga gcgcaccaac ggccccaact tcaaccagcg
     3361 caaggtcagc cgcgacgacg cgctctacgc catccgcaac ggcgggttct cgggcgcgat
     3421 catgccggcc aacatcgtga ccggcaggga agccgaccgg ctggccgact acctggccaa
     3481 gtactccggc cgcgagcgca ccagcgacgc caactaggcg gccggctcgg tcgccggctc
     3541 cggcgacggc ttcacgcgca gcagcttcgc cggccccggc ggcagccacc agttccagcg
     3601 cccgaacatc gacaccaccg cgggcaccag cagcgcccgc accacggtgg cgtccagcag
     3661 gatccccgcc gccaggcccg tggccaggat cttcacgtcg gtctcggggc ccgacgccat
     3721 cgaggcgaag gccaggaaca ggatcagcgc ggcgctggtg accaggcggc cggtgcgggc
     3781 caggccctcg atcaccgcct tgtcggtgtc gcctcccttg tcgaactcct cgcgcatgcg
     3841 cgagaggatg aagacctcgt agtccatcga cagcccgtac aggaacgcga acaccatcag
     3901 cgggatccac gaggcgatcg agccggtggc cgcgatgccc cagatctcgt ccgagccgtg
     3961 gccgttctgc cagatcagcg ccatcacgcc ccacgcggcg cccacggaca ggatgttcag
     4021 gatcaccgcc ttcagcggca gcagcagcga ccggaacgcg cgcgccagca gcaggaaggt
     4081 cagcagtgag atcaggccga tcatcagcgg gaaggcgccg tagacggcgt cgatgaagtc
     4141 ggcgttcagc ggtcccgacc cgcccacgcg cacgtcgggc ccaaccgcgt gcgaggcgtc
     4201 gcgcacctcg tcgacggccg cccgcccctc ggccgtctcg tcgccctcgt tgggcacgcc
     4261 cgcgatcagc gcctccccgt tcttgcgcca ggccgggttc tggggcgcca ccgcgccgtg
     4321 catgccgtcg acaccctgct gggccttgac caccgcgtcg gcgtcggcca cgggcgccag
     4381 cgtctcgaac ggcgcgatcg cgccggtgcc gatgcccgag tcgcgcaggt cgtgcaggcc
     4441 ctccttggcc gggcccgcct tggcgatggt gtccgggtcg gagttgccca ggtgcaggtc
     4501 ggtggccggg atcaggagcg cgaccaggat ggccacggcg atgacggcgg cgacgccccg
     4561 gtggcgcacg atcccctcgg cccagccctg ccagaagcgc gcgctgcggt cgctgcggcg
     4621 gatgcgccgc cggtccagcc gcgggccgat ggtggccagc accaccggca gcagcgtgat
     4681 cgcggccagc gtggccacca gcgggatgat catcccgccg tagcccatgc tgcgcaggaa
     4741 gggcagcggc aacgcgatca gggccagcag gccgacggcc accgtcgtgc ccgagaacac
     4801 cacggcgcgc ccggccgtgc tcatggccgc ggccacggcg tcgtcgccct ccctgccgcg
     4861 ctcgagctcc tcgcgccagc gcaccaccac cagcagcgcg tagtcgatcg agatgcccag
     4921 gccgaccagc gccaccagga actgcacgat cggcgagacc tcggtgatcg cggtcagcgc
     4981 gtacagcagc aggaccgaga ccaggatcga gcagacggcc atggccaggg ggacgaaggc
     5041 cagcgccgag ccgaacacga acaccagcac caccagggcg cccacgccgc ccagcacggc
     5101 ctccagcagc acgccggggc cgtcgtcgcc ctcgcccgag gagtcgaaca gcgcgtcgta
     5161 gccggtcagg cgcacgggtg cccccgccac ctcggcgccg gccagcgcgc tgcgcagctc
     5221 gcgcatgacc tcggtgttgg cgccgaacgg gtcgtcgctg agcggggtga aggcgtagac
     5281 gaacgcggtc cgcccgtcct cggacacgaa cgtccggtcg ccggtcgagc cgaagcccgc
     5341 cacgcgcgat ccgggcacgg tcttcttcgc
//
LOCUS       DASUPW010000547         3147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_679582, whole genome shotgun sequence.
ACCESSION   DASUPW010000547 DASUPW010000000
VERSION     DASUPW010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679582"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VFY44_12345"
     CDS             <1..402
                     /locus_tag="VFY44_12345"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HEX5911275.1"
                     /translation="IWFDSGTVYLSTTADSRIHAYDTRRGRMQVIYDGLASRDAPLTR
                     VDNLTASKAGELFVCEDISTEEIHIGVITPRRKASRFLAVSGGNHVGSELTGIAFNPA
                     GDRLYFSSQRASDLKGEVYEVSGPFRGRRRA"
     gene            complement(399..1130)
                     /gene="cofE"
                     /locus_tag="VFY44_12350"
     CDS             complement(399..1130)
                     /gene="cofE"
                     /locus_tag="VFY44_12350"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEX5911276.1"
                     /translation="MSLTATALEGIPEVRAGDDLAALLAATAGFAAGDIVVVAHKVVS
                     KAEGRVRDLATVEPGERARDLASEHDKDPRLVQVVLDESAEVLRAHHGVLICLTRHGF
                     VCANAGVDRSNSPGDDQVVLLPTDPDASARALRAALPGRPAVVVTDSFGRAWRVGQTD
                     VAIGAAGLQPLDDWRGRPDARGREMEATEIAVADAVAGAADLARSKDGGTPAVLVRGL
                     ERFVTEGDGPGAAALRRPPELDLFR"
     gene            complement(1127..1765)
                     /gene="cofC"
                     /locus_tag="VFY44_12355"
     CDS             complement(1127..1765)
                     /gene="cofC"
                     /locus_tag="VFY44_12355"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEX5911277.1"
                     /translation="MRTAAILPVKSFGSAKQRLGELIEQDARSALMRAMLADVLDALS
                     EVPELEQVLVVTANPLAGELAGDPRIQVLHDDREAGQSAAAQIGIARARDAGFDRALL
                     VPGDAPLLDPAELSGLLARPEPLVIVPDRHGTGTNGLLLAPVDALEPSFGPGSLARHE
                     AAARATGLPWAVDPLPSLVLDVDTPEDLEATTARPGGAPATRAALRRLSLPA"
     gene            complement(1773..2681)
                     /gene="cofD"
                     /locus_tag="VFY44_12360"
     CDS             complement(1773..2681)
                     /gene="cofD"
                     /locus_tag="VFY44_12360"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEX5911278.1"
                     /translation="MEVALLSGGTGGAKLARGLLDAIGPENLAVIANTGDDAEVYGAH
                     VSPDPDLVTYWLADVIDERGYGIAGDTWQVMEALAGAGRETWFRLGDRDLAMCLIRTE
                     LMEQGHSATAAHAAVVEAMGVRARVLPATDQPVRTRVGARGTTFAFQEFMIVEGARGP
                     IESVEFEGADHARPTEPVLGALREADAVVIGPSNPIASIAPILAVPAMREAVRAAPGP
                     VVAVSPFVGGQVLKGPTVAFCDHAGIEPTAAGLLDAYEGLLDGIVADEPVAGIPALRT
                     DTLMDTPQARARLAAETVDFARSLSS"
     gene            complement(2672..>3147)
                     /locus_tag="VFY44_12365"
     CDS             complement(2672..>3147)
                     /locus_tag="VFY44_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911279.1"
                     /translation="FLTVPFRAQSENLTNLKEALTAGQILVDATVPLAAAVSGKATRL
                     LGVPQGSAAEQAQEMVPDGVTVVSALHSVSAATLADPEAELDEDVPVCGQKKDDKARV
                     AAIIGSIPGLRPVNAGALETARLVEGLTPLLISVNIRNKTHAGIKFTGLPEDLWK"
BASE COUNT          395 a         1209 c         1152 g          391 t
ORIGIN      
        1 atctggttcg acagcgggac cgtctacctg tcgaccaccg ccgactcgcg catccacgcc
       61 tacgacacgc gccggggccg gatgcaggtc atctacgacg gcctggcctc gcgcgacgcg
      121 ccgctgacgc gggtcgacaa cctgaccgcg tcgaaggccg gcgagctgtt cgtgtgcgag
      181 gacatctcga ccgaggagat ccacatcggg gtgatcacgc cgcggcgcaa ggcctcgcgc
      241 ttcctggccg tcagcggcgg caaccacgtg ggctccgagc tcacgggcat cgccttcaac
      301 cccgccggcg accggctcta cttctcctcc cagcgcgcca gcgacctcaa gggtgaggtc
      361 tacgaggtct cgggcccctt ccgcggccgc cgcagggcct agcggaagag gtcgagctcc
      421 ggcgggcggc gcagggccgc agcgcccggc ccgtcgccct cggtcacgaa ccgctccagg
      481 ccgcgcacca gcaccgcggg ggtgccgccg tccttggagc gggccaggtc cgcggcgccg
      541 gccaccgcgt cggccacggc gatctcggtg gcctccatct cccggccgcg cgcgtcgggg
      601 cggccgcgcc agtcgtccag cggctggagg cccgcggcgc cgatggccac gtcggtctgg
      661 cccacccgcc aggcgcggcc gaaggaatcg gtgaccacca cggcggggcg gccgggcagg
      721 gcggcgcgca gcgcgcgcgc cgacgcgtcg gggtcggtgg gcaacagcac cacctggtcg
      781 tcgcccgggg agttggagcg gtcgacgccc gcgttggcgc acacgaagcc gtggcgggtc
      841 aggcagatca gcacgccgtg gtgggcgcgc agcacctcgg ccgactcgtc cagaaccacc
      901 tgcaccagcc gcgggtcctt gtcgtgctcg gaggccaggt cgcgcgcgcg ctcgccgggc
      961 tcgactgtgg ccaggtcgcg cacgcggccc tcggccttcg agaccacctt gtgggccacc
     1021 accacgatgt cgccggcggc gaacccggcc gtggccgcca gcagcgcggc caggtcgtcg
     1081 cccgcgcgca cctcggggat cccctccagc gcggtggcgg tgaggctcac gcgggcagcg
     1141 acaggcgccg cagcgccgcc cgggtcgcgg gcgcgccgcc gggccgggcg gtcgtggcct
     1201 ccaggtcctc gggcgtgtcc acgtccagga ccagggaggg gagggggtcg accgcccagg
     1261 gcaggcccgt ggcccgcgcg gccgcctcgt ggcgggccag gctgcccggc ccgaaggacg
     1321 gctcgagggc gtccactggc gccagcagca ggccgttggt gccggtcccg tggcggtcgg
     1381 gcacgatcac cagcggctcg ggccgcgcca gcagccccga cagctcggcc gggtccagca
     1441 gcggcgcgtc gccgggcacc agcagcgccc ggtcgaagcc ggcgtcgcgg gcacgggcaa
     1501 tgccgatctg cgcggctgcg gactggcccg cctcgcggtc gtcgtgcagc acctggatcc
     1561 gggggtcgcc cgccagctcg cccgccagcg ggttcgcggt caccaccagc acctgctcca
     1621 gctcgggcac ctccgacagc gcgtccagca cgtcggccag catcgcgcgc atcagcgcgc
     1681 tgcgcgcgtc ctgctcgatc agctcgccca ggcgctgctt ggcggagccg aagctcttga
     1741 ccgggaggat cgcggcggtg cgcatgaccg cttcaggatg acaaggagcg ggcgaagtcc
     1801 accgtctccg ccgccagccg ggcccgcgcc tgcggcgtgt ccatcagcgt gtcggtacgc
     1861 agcgccggga tcccggccac cggctcgtcg gccacgatgc cgtccagcag cccctcgtag
     1921 gcgtccagca gcccggccgc ggtgggctcg atgccggcgt gatcgcagaa ggcgaccgtc
     1981 ggccccttca gcacctgccc gcccacgaac gggctgaccg ccaccaccgg gccgggtgcc
     2041 gcccgcaccg cctcgcgcat cgccggcacg gccaggatcg gcgcgatcga ggcgatcggg
     2101 ttcgacggcc cgatgaccac cgcgtccgcc tcgcgcagcg cgcccagcac cggctcggtc
     2161 ggccgggcgt ggtcggcgcc ctcgaactcg acgctctcga tcgggccgcg cgcgccctcc
     2221 acgatcatga actcctggaa ggcgaaggtc gtgccgcgcg cgcccacgcg cgtgcgcacc
     2281 ggctggtcgg tggcgggcag cacccgcgcc cggaccccca tcgcctcgac cacagcagcg
     2341 tgcgcggccg tggcggagtg gccctgctcc atcagctcgg tgcggatcag gcacatggcc
     2401 aggtcgcggt cgcccaggcg gaaccaggtc tcgcggccgg cgccggccag cgcctccatg
     2461 acctgccacg tgtcgccggc gatcccgtag ccgcgctcgt cgatcacgtc ggccagccag
     2521 taggtcacca ggtccgggtc gggcgacacg tgcgcgccgt acacctctgc gtcgtcgccg
     2581 gtgttggcga tcaccgccag gttctccggc ccgatggcgt ccagcagccc gcgggccagc
     2641 ttggccccgc cggtgccgcc ggagagcagt gctacttcca gaggtcttcg ggcaggccgg
     2701 tgaacttgat cccggcgtgc gtcttgttgc ggatgttcac ggagatcagc aggggggtga
     2761 ggccctcgac cagccgcgcc gtctccagcg cgccggcgtt cacagggcgc aggccgggga
     2821 tcgagccgat gatggccgcc acgcgcgcct tgtcgtcctt cttctggccg cacaccggca
     2881 cgtcctcgtc cagctcggcc tccgggtcgg ccagcgtggc cgccgacacc gagtgcagtg
     2941 ccgagaccac ggtcacgccg tcgggcacca tctcctgcgc ctgctcagcg gccgagccct
     3001 ggggcacgcc cagcagccgg gtggccttgc ccgagacggc ggcggccagg ggcacggtgg
     3061 cgtccaccag gatctgcccg gcggtgagcg cctccttgag gttggtcagg ttctcggact
     3121 gggccctgaa ggggacggtc aggaaga
//
LOCUS       DASUPW010000548         3461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1372203, whole genome shotgun sequence.
ACCESSION   DASUPW010000548 DASUPW010000000
VERSION     DASUPW010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372203"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1749)
                     /locus_tag="VFY44_12370"
     CDS             complement(<1..1749)
                     /locus_tag="VFY44_12370"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX5911280.1"
                     /translation="MTTAAAPEALLEGLNEPQRQAVTHGEGPLLILAGAGSGKTRALT
                     RRIAYLVGTGQAHPSEILAITFTNKAAAEMRERVEVLVGRRTRAMWVMTFHAACARIL
                     RSDAERLGYTRGFTIYDEADSVRLIKQCMDELGTDPKRFPPRAIKRQISDAKNQLLDA
                     DAYRLKMNGYFEQTAAEVYELYEQRIHGMNAMDFDDLLFQSVNLFQLFPEVLERYRRS
                     FKHVLVDEYQDTNHAQYQWLRLLAEEHRNLAVVGDDSQSIYAFRHADIRNILEFEHDY
                     PDAEVVKLEQNYRSTQTILDAANAVINNNRAQIEKHLWTQNGKGEPIHMREMEDEHSE
                     ARYVAGQIERHVEGGGSRNDVAVFYRANAQSRVLEDTLVRYGVSYQVIGGTRFYERAE
                     IKDALSYLTLLTNPQDVVAFQRVVNSPKRGIGDTSQARIVGHANTLGEPVFDVALDPA
                     AVPGLGTAAVKAVSRFMETMEKLRDGAEGASVGDLLQDTLSEVGYIDALENERTIESQ
                     GRLENLEELVSVAREYDASAEDPGVEEFLQQVSLFSAQDSIEDDEGVVTLMTLHNAKG
                     LEYDVVFLIGLEDGVFP"
     gene            complement(1776..2132)
                     /locus_tag="VFY44_12375"
     CDS             complement(1776..2132)
                     /locus_tag="VFY44_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911281.1"
                     /translation="MRAPAAFFVVAALALGGCGGSDPSDEDQVRKAISSYYEALGGDE
                     PEKVCDLLVTARGELPPPRCRKRMARVPDAISVGLPLKVRSVRVRGAGATAALEDGER
                     VRLRRVDDAWRIVAPG"
     gene            2153..3013
                     /locus_tag="VFY44_12380"
     CDS             2153..3013
                     /locus_tag="VFY44_12380"
                     /inference="COORDINATES: protein motif:HMM:NF021832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine salvage family protein"
                     /protein_id="HEX5911282.1"
                     /translation="MALCDDVRERCAAVAASATRVRIDADRLDAIEPGPAPALDPASH
                     YLDGPEEEVALFTLELDAINFGSGWFPTIRKRPGCSGSVSIARAFTEHGPWPAAELSR
                     LDAPTVAAALGQDPGHVLMGLYAVALNELGEFLGDRTALDVVAEAGGSAQALAAQVAD
                     AMPMFADPGFYKRAQILPADLALAGVAEFDDLDALTIFADNLVPHVLRVDGVLVYDAE
                     LAGLIDSGELLPPGPAEREIRACAVHACELLAARMAVPPRTLDNWLWNRGQQLRYKAL
                     PRHRTRTTAY"
     gene            complement(3010..>3461)
                     /locus_tag="VFY44_12385"
     CDS             complement(3010..>3461)
                     /locus_tag="VFY44_12385"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5911283.1"
                     /translation="VLQATLEAHDGDPRRLTVEITETAAISDLDRAQAFAQALNAMGV
                     RLALDDFGAGFASFQHLTRLHFDEIKIDGEYVRNLADDETHQVLVRSLAELARGLGKD
                     TTAEFVQDDRTIELLRQYGVRWGQGFHLGRPAPLDALGVVDLPVAAA"
BASE COUNT          453 a         1231 c         1235 g          542 t
ORIGIN      
        1 cgggaacacc ccgtcctcca agccgatcag gaacaccacg tcgtactcca ggcccttggc
       61 gttgtgcagc gtcatcagcg tgaccacgcc ctcgtcgtcc tcgatggagt cctgggcgct
      121 gaacagcgag acctgctgga ggaactcctc gaccccgggg tcctcggccg acgcgtcgta
      181 ctcgcgggcc acgctgacca gctcctccag gttctccagc cggccctggc tctcgatggt
      241 gcgctcgttc tcgagcgcgt cgatgtagcc cacctcgctg agcgtgtcct gcagcaggtc
      301 accgaccgac gccccctccg ccccgtcgcg cagcttctcc atggtctcca tgaagcggct
      361 gaccgccttg accgccgcgg tgcccagccc gggcacggcc gccgggtcca gcgcgacgtc
      421 gaacacgggc tcaccgagcg tgttggcgtg gcccacgatc cgcgcctgcg aggtgtcgcc
      481 gatgccgcgc tttggcgagt tgaccacgcg ctggaaggcg accacgtcct gcgggttggt
      541 cagcagcgtc aggtaggaca gcgcgtcctt gatctcggcg cgctcgtaga agcgggtgcc
      601 gccgatgacc tggtagctga cgccgtagcg caccagcgtg tcctcgagca cgcggctctg
      661 ggcgttggcg cggtagaaga cggccacgtc gttgcgcgag ccgccgccct ccacgtggcg
      721 ctcgatctgc ccggcgacgt agcgcgcctc ggagtgctcg tcctccatct cgcgcatgtg
      781 gatcggctcg cccttgccgt tctgggtcca caggtgcttc tcgatctggg cgcggttgtt
      841 gttgatgacc gcgttcgcgg cgtccaggat cgtctgcgtc gagcggtagt tctgctcgag
      901 cttgaccacc tcggcgtcgg ggtagtcgtg ctcgaactcc aggatgttgc ggatgtcggc
      961 gtggcggaag gcgtagatgg actggctgtc gtcgccgacc accgccaggt tgcggtgctc
     1021 ctcggccagc agccgcagcc actggtactg ggcgtggttg gtgtcctggt actcgtccac
     1081 cagcacgtgc ttgaagctgc gccggtagcg ctcgagcacc tcggggaaga gctggaacag
     1141 gttcaccgac tggaacagca ggtcgtcgaa gtccatggcg ttcatgccgt ggatgcgctg
     1201 ctcgtagagc tcgtagacct cggcggcggt ctgctcgaag tagccgttca tcttgagccg
     1261 gtaggcgtcg gcgtccagca gctggttctt ggcgtccgag atctggcgct tgatcgcccg
     1321 cggcgggaag cgcttggggt cggtgcccag ctcgtccatg cactgcttga tcagccggac
     1381 cgagtcggcc tcgtcgtaga tcgtgaagcc gcgcgtgtag cccagccgct cggcgtcgga
     1441 gcgcaggatg cgcgcgcacg cggcgtggaa ggtcatgacc cacatcgcgc gggtgcgccg
     1501 gccgacgagg acctcgacgc gctcgcgcat ctcggccgcg gccttgttgg tgaaggtgat
     1561 ggccaggatc tccgaggggt gcgcctggcc cgtgcccacc aggtaggcga tccggcgcgt
     1621 cagcgcgcgc gtcttgcccg acccggcccc ggccaggatc aggagcggcc cctcgccatg
     1681 ggtcaccgcc tggcgctggg gttcgttcag gccttcgagc agggcctctg gcgcggcggc
     1741 ggtggtcacc gatccaaggt taggggcgac gccggtcagc ccggggccac gatccgccat
     1801 gcgtcgtcca cccggcgcag gcgcacgcgc tcgccgtcct cgagcgcggc ggtcgcgccg
     1861 gcgccacgga cgcggaccga ccgcaccttc agcggcaggc cgaccgagat cgcgtccggc
     1921 acacgggcca tccgcttccg gcaccgaggg ggcgggagct cgccgcgcgc cgtgaccagc
     1981 aggtcgcaca ccttctcggg ctcatccccg cccagggcct cgtagtacga ggagatcgcc
     2041 ttgcgcacct ggtcctcgtc gctggggtcg gaccccccac agccgcccag cgcgagcgcg
     2101 gcgacgacga aaaaggcggc aggcgctcgc atcagcggtc aggctagggg tcatggccct
     2161 gtgcgacgac gttcgtgagc gctgtgccgc cgtcgcggcg tccgcgaccc gggtgcggat
     2221 cgacgccgac cgcctggacg cgatcgagcc cggccccgcc cccgcgctgg acccggcgag
     2281 ccactatctg gacggcccgg aggaggaggt ggccctgttc acgctggagc tggacgccat
     2341 caacttcggc tcgggctggt ttcccaccat ccgcaagcgt cccggctgct cggggtcggt
     2401 cagcatcgcc cgggcgttca ccgagcacgg gccgtggccg gccgcggagc tgtcgcggct
     2461 ggacgccccc acagtggccg cggcgctggg ccaggatccc ggccacgtgc tcatgggcct
     2521 gtacgcggtc gcgctgaacg agctgggcga gttcctcggc gaccgcaccg cgctggacgt
     2581 ggtcgccgag gcgggcgggt cggcccaggc cctggccgcg caggtggccg acgccatgcc
     2641 catgttcgcc gaccccggct tctacaaacg ggcccagatc ctgcccgccg acctggccct
     2701 ggccggcgtg gccgagttcg acgacctgga cgcgctgacg atcttcgccg acaacctggt
     2761 cccccacgtc ctgcgcgtgg acggggtgct ggtctacgac gccgagctgg cgggcctgat
     2821 cgactcgggc gagctgctgc cgccgggccc ggccgagcgt gagatccgcg cgtgtgcggt
     2881 gcacgcctgc gagctgctgg cggcacggat ggccgtgccg ccccggaccc tggacaactg
     2941 gctctggaac cggggccagc agctgcgcta caaggcgctg ccgcgccacc ggacgcgcac
     3001 gacggcctac taggccgccg cgacgggcag gtcgaccacg ccgagggcgt ccagcggggc
     3061 gggccggccc aggtggaagc cctggcccca gcgcacgccg tactggcgca gcagctcgat
     3121 cgtgcggtcg tcctggacga actcggccgt cgtgtccttg cccaggccgc gggccagctc
     3181 ggccagcgag cgcaccagga cctggtgggt ctcgtcgtcg gccaggttgc gcacgtactc
     3241 gccgtcgatc ttgatctcgt cgaagtgcag ccgggtcagg tgctggaagg aggcgaaccc
     3301 ggccccgaag tcgtccagcg ccaggcgcac gcccatcgcg ttcagcgcct gggcgaaggc
     3361 ctgggcccgg tcgaggtcgg agatcgccgc cgtctcggtg atctccacgg tcaggcgccg
     3421 gggatcgccg tcgtgggcct cgagcgtcgc ctgcaggacc t
//
LOCUS       DASUPW010000549         3211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1511972, whole genome shotgun sequence.
ACCESSION   DASUPW010000549 DASUPW010000000
VERSION     DASUPW010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511972"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="VFY44_12390"
     CDS             complement(<1..1020)
                     /locus_tag="VFY44_12390"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HEX5911284.1"
                     /translation="MSKTLIVAEKPSVARDLAGALPDSFKQSKDKTSLEGDDYVVTWA
                     VGHLVGLAEPDAYDPKLKKWRFADLPIVPDEFKLVPNDDRAKKQLNAIHKLMARDDVD
                     QVINACDAGREGELIFAYVYETAKKQPPVKRLWLSSMTKKAIADAFTSLRDGEEMHSL
                     EDAARSRSEADWVVGMNATRAASIRLRAAFDGAVSLGRVQTPTLALVARREEEIRAFK
                     PEPYWLVEAKFGATGDRRYSGRYMGGKRIAEDEAATIVKEVEGQSGTITKLEKKEETE
                     KSQILYDLTSLQRHANTLLGFSARRTLSAAQKLYEEHKALTYPRTNSRFLTGDMAGEI
                     KPIAKL"
     gene            1130..1513
                     /locus_tag="VFY44_12395"
     CDS             1130..1513
                     /locus_tag="VFY44_12395"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5911285.1"
                     /translation="MDQASVRYIVHDVDAAVGFYRDLLGFEVDMHPGPGFAALTRGPL
                     RLLLNAPGGPGGAAQPTPDGRAPEPGGWCRFQVTVADIEAEVERLTGAGARFRNEIVT
                     GRGGSQILLDDPSGNAIELFQPAGG"
     gene            1517..1807
                     /locus_tag="VFY44_12400"
     CDS             1517..1807
                     /locus_tag="VFY44_12400"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX5911286.1"
                     /translation="MREFLRGAVQLHVLHHAAEDEVDGAWMSAELAHHGYSISPGTLY
                     PTLHRMEAEGLLRSRAVVVDGRRRRRYSATDEGRRTLEQTRRALRELADEVL"
     gene            complement(1817..2665)
                     /locus_tag="VFY44_12405"
     CDS             complement(1817..2665)
                     /locus_tag="VFY44_12405"
                     /inference="COORDINATES: protein motif:HMM:NF013360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HEX5911287.1"
                     /translation="MQYRSLLALAGVLGVLVVAPDPASAQKPPNPCLDALQRAALRCP
                     DLKMRKPFGLRVDPFVRPGRVMLRAGNSIDSVGAGPAELFGVRSSRMYMKARQRIYRR
                     AKGQRLGVSTGARLYFKYIPRQRSYWKFLHAAQFDLYRLNANGSRGKRVRRGPKVSYC
                     LRDLEHTTPGRARSPRKRVYPACNTSSRTRKVRLGTSVGWSDVYPPGYPEQYIDVTGL
                     RGCFAYVHTADPRNGIYESNERNNASTVVVRLPFRGGRQRCPGPQASSVPDTRNYDAE
                     YSDVYR"
     gene            complement(2692..>3211)
                     /locus_tag="VFY44_12410"
     CDS             complement(2692..>3211)
                     /locus_tag="VFY44_12410"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5911288.1"
                     /translation="SDGVVVTNAHVIAGQSDTSVQLGGRGERLDAQAIHFDVANDLAV
                     LRVPGVSGVRPLAMNEGARPGTGAAVLGFPENGPYDVEPARVGTTSTVLSQDAYGRGP
                     VRRRITALRGLVRSGNSGGPAVDAQGRVVTTIFAAARESGSGSGFGVPDSIVRQALSR
                     ARGPVSTGPCSR"
BASE COUNT          425 a         1113 c         1197 g          476 t
ORIGIN      
        1 cagcttggcg atcggcttga tctcgccggc catgtcgccg gtcaggaagc gcgagttcgt
       61 acgcgggtag gtcagcgcct tgtgctcctc gtagagcttc tgcgcggccg acagggtgcg
      121 gcgcgccgag aagcccagca gggtgttggc gtggcgctgg agggaggtca ggtcgtagag
      181 gatctgcgac ttctcggtct cctccttctt ctccagcttg gtgatggtgc cgctctggcc
      241 ctccacctcc ttgacgatcg tggccgcctc gtcctcggcg atccgcttgc cgcccatgta
      301 ccggccggag tacctgcggt cgccggtggc gccgaacttg gcctcgacca gccagtaggg
      361 ctcgggcttg aacgcgcgga tctcctcctc gcggcgggcc accagcgcca gcgtgggcgt
      421 ctggacccgg cccagggaca cggcgccgtc gaacgcggcg cgcagccgga tcgaggccgc
      481 gcgggtggcg ttcatgccca cgacccagtc ggcctccgag cgcgagcggg cggcgtcctc
      541 gagcgagtgc atctcctcgc cgtcacgcag cgaggtgaac gcgtccgcga tcgccttctt
      601 ggtcatcgag gacagccaca ggcgcttgac cggcggctgc ttcttggcgg tctcgtacac
      661 gtaggcgaag atcagctcgc cctcgcgccc ggcgtcgcag gcgttgatga cctggtccac
      721 gtcgtcgcgg gccatcagct tgtggatggc gttcagctgc ttcttcgcgc ggtcgtcgtt
      781 gggcacgagc ttgaactcgt ccgggacgat cggcaggtcg gcgaagcgcc acttcttgag
      841 cttcgggtca taggcgtcgg gctcggccag cccgaccagg tggccgaccg cccaggtgac
      901 cacgtagtcg tcgccctcca ggctggtctt gtccttggac tgcttgaagg aatcgggcag
      961 cgcgccggcg aggtcgcggg cgaccgacgg cttctcggcg actatgaggg tcttggacat
     1021 cagcggggaa ccttaggcag tcgggcgagg gcgatggtag gcggtacggc gggcgggtag
     1081 tatcggtttc cgatatcgga cttcgatacc aacgctggag gaggctccca tggaccaggc
     1141 aagcgtgcgc tacatcgtgc acgacgtcga cgcggccgtc ggcttctacc gcgacctgct
     1201 cgggttcgag gtggacatgc accccgggcc gggcttcgcc gcgctcacgc gcggaccgct
     1261 gcggctgctc ctgaacgcgc ccggcggtcc cggcggagcg gcgcagccca cccccgacgg
     1321 ccgtgcgccc gagcccggcg gctggtgccg gttccaggtc acggtcgccg acatcgaggc
     1381 cgaggtcgag cgtctgacgg gcgcgggcgc acggttccgg aacgagatcg tcaccggccg
     1441 cgggggcagc cagatcctcc tggacgaccc gtccggcaac gcgatcgagc tgttccagcc
     1501 ggccggcggc tgacgggtgc gcgagttcct gcggggagcg gtccagctgc acgtgctcca
     1561 ccacgccgcc gaggacgagg tcgacggggc ctggatgagc gcggagctgg cacaccacgg
     1621 ctacagcatc agccccggca cgctgtaccc caccctccac cggatggagg cggagggcct
     1681 gctgcggtcg cgggcggtcg tggtcgacgg gcgtcgccgg cgccgctaca gcgccaccga
     1741 cgagggccgc cgcacgctgg agcagacgcg ccgtgccctg cgcgagctgg ccgacgaggt
     1801 gctctgagcg gcgcgcctag cggtagacgt ccgagtactc ggcgtcgtag ttccgggtgt
     1861 cgggcacgga ggacgcctgg gggcccgggc agcgttgccg gccgcccctg aagggcagcc
     1921 gcacgaccac cgtcgaggcg ttgttgcgct cgttggactc gtagatgccg ttgcgcgggt
     1981 cggcggtgtg cacgtaggcg aaacagccgc gcaggccggt cacgtcgatg tactgctcgg
     2041 ggtagccggg cgggtacacg tccgaccagc ccaccgacgt gcccagccgg accttccgcg
     2101 tgcgcgagct ggtgttgcac gcggggtaca cgcgcttgcg cggcgaccgc gccctgcccg
     2161 gggtggtgtg ctccaggtcg cgcaggcagt aggagacctt cggcccgcgc cgcacccgct
     2221 tgccgcggct gccgttggcg ttcaggcggt agaggtcgaa ctgggcggcg tggaggaact
     2281 tccagtagct gcgctgacgc gggatgtact tgaagtacag ccgcgccccg gtggacacgc
     2341 cgagccgctg gcccttggcg cggcggtaga tccgctggcg cgccttcatg tacatgcgcg
     2401 aggagcgcac gccgaacagc tcggccggcc cggcgcccac gctgtcgatg gagttgccgg
     2461 cccgcagcat gacccggccc gggcgcacga acgggtccac gcgcaggccg aacggcttgc
     2521 gcatcttcag gtcggggcag cgcagcgcgg cgcgctggag ggcgtcgagg caggggttgg
     2581 gcggcttctg ggcggacgcg gggtcgggag ccaccacgag caccccgagc acgccggcca
     2641 gggcgaggag cgagcggtat tgcacggcgg aattcatagc gagtcggctc gtcagcgaga
     2701 acatgggccg gtcgacaccg gcccgcgggc gcggctcagc gcctggcgca cgatcgagtc
     2761 cgggaccccg aacccgctcc cgctgcccga ctcccgcgcg gcggcgaaga tcgtggtcac
     2821 cacccgtccc tgggcgtcca cggcagggcc gccggagttg cccgaccgga ccaggccgcg
     2881 cagcgcggtg atccggcgcc gcaccgggcc gcggccgtag gcgtcctggc tcaggaccgt
     2941 gctcgtggtg ccgacccggg ccggctccac gtcgtagggg ccgttctcgg ggaagccgag
     3001 cacggcggcg ccggtgcccg gtcgtgcccc ctcgttcatc gccagcggtc gcacgccgga
     3061 cacgcccggc acgcgcagga ccgccaggtc gttggccacg tcgaagtgga tcgcctgggc
     3121 gtccaggcgc tcgccccggc cgcccagctg caccgacgtg tcgctctgcc ccgcgatcac
     3181 gtgggcgttg gtgaccacca ccccgtcgga g
//
LOCUS       DASUPW010000550         3053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1236151, whole genome shotgun sequence.
ACCESSION   DASUPW010000550 DASUPW010000000
VERSION     DASUPW010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236151"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VFY44_12415"
     CDS             <1..718
                     /locus_tag="VFY44_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911289.1"
                     /translation="ELGALRFSPGAEDWIEYGPEDDRRRIDFHDYAAIYAEPGLYEHV
                     FYDTLRMRSTGEVVRLFGEVLAELGLDPAQQRVVDFGAGNGLGGEGLRALGVGELVGL
                     DLEPMARTAAERDRPGVYGDYLVGDLGAWSEEELDRLRAHRPTAIVALSAVGVGHVPR
                     ATLARALGLLGPGGVYAFAVAPPLLPESTDPEGQATGYPGYLRSLIEETELLRRHEYV
                     HRYRPDGSEDLGIAFVGRMP"
     gene            complement(661..1455)
                     /locus_tag="VFY44_12420"
     CDS             complement(661..1455)
                     /locus_tag="VFY44_12420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX5911290.1"
                     /translation="MSSCLRCCVVVPARDEEDLIGACLDALAAQRGVPAPAWEVIVVL
                     DACGDDTAGEVARATVRNPGLAVTVLDGPGRGAGPARRVGMDAAAARLPAEGLIACTD
                     ADSTVAPDWIAAQLRAAAAGARAIGGRIEIAPAEMAALPVDVVVDRLRRQHRRHQLVL
                     ADPGPPGSTSEHWQFSGASLAVTAEVYRRVGGMGPDRALEDEAFERALIDHGVPIQRS
                     LDVRVTTSGRLVGRAERGLAHDLARAARQGIRPTNAMPRSSEPSGR"
     gene            complement(1452..2621)
                     /locus_tag="VFY44_12425"
     CDS             complement(1452..2621)
                     /locus_tag="VFY44_12425"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5911291.1"
                     /translation="MSTGRGVVIVGGGPAGLATARAFREAGGDGPVTIVAAEPHAPYE
                     RPPLTKEFLRGETAEHDLPIEDELWFARHKVDLRTGVRATALDLAGRQVALDDGEHLD
                     YEHCVLATGSEPQRLPVPGADDPGVLTMRTIEDARALQRHGRRVVVVGSGFVGCEAGA
                     SLAARGAAVTVVTAEALPQSERLGPEVGGRITTWMEDAGVELMAGTELSRIERDGSTW
                     RVQAGDHAELDADTVLMAVGVAPRVGLAREAGLRVEGGGVATGADMRTSDPAVLAVGD
                     VAYAHNAAAGRPLPVEHWGEALHQGAVAGAVLAGEDRAWANAPGFWSAIADRTLKYVA
                     WGDGYSSVRLDAGDDGGFTAWYGDDDGTCVGVLCHRRDDDYERGRELVESGAPTP"
     gene            complement(2618..>3053)
                     /locus_tag="VFY44_12430"
     CDS             complement(2618..>3053)
                     /locus_tag="VFY44_12430"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX5911292.1"
                     /translation="RALEVGCSIGVFTQALAGVARELVAVDVSEAAVAAARERTARLD
                     NVSVRQATLPEDMPPGRFDLIVCSEVLYYWDERLLEHGLDTLAGALEPEGVLLAVHWT
                     EPTRDYPLQGHQVHDALARRPDLAPVHGHDGSHYRLDLFRRA"
BASE COUNT          355 a         1202 c         1133 g          363 t
ORIGIN      
        1 cgaactgggc gcgctgcgct tctcccccgg ggccgaggac tggatcgagt acgggcccga
       61 ggacgaccgg cgccggatcg acttccacga ctacgcggcc atctacgccg agcccggcct
      121 ctacgagcac gtcttctacg acaccctccg catgcgctcc accggcgagg tggtccggct
      181 gttcggcgag gtgctggccg agctggggct ggacccggcg cagcagcggg tggtcgactt
      241 cggcgccggc aacgggctcg ggggcgaggg cctgcgcgcg ctcggcgtcg gcgagctggt
      301 cgggctggac ctggagccga tggcccggac ggcggccgag cgcgatcggc ccggtgtgta
      361 cggggactac ctggtcggcg acctgggcgc ctggtccgag gaggagctcg accgcctgcg
      421 cgcccaccgg ccgaccgcga tcgtggccct ctccgccgtg ggcgtgggac acgtgcccag
      481 ggcgaccctg gcgcgtgcgc tggggctgct gggccccggc ggcgtgtacg cgttcgcggt
      541 cgcgcccccg ctgctgcccg agagcaccga cccggagggg caggccacgg gctaccccgg
      601 ctacctacgc tcgctgatcg aggagaccga gctgttgcgc cggcacgagt acgtgcaccg
      661 ctaccggccg gacggctcgg aggacctggg catcgcgttc gtgggccgga tgccctgacg
      721 ggcggcccgc gccaggtcgt gggccaggcc gcgctcggcg cgcccgacca gcctcccgct
      781 cgtggtcacc cgcacgtcca gcgaccgctg gatgggcacg ccgtggtcga tcagcgcacg
      841 ctcgaacgcc tcgtcctcca gcgcacggtc gggccccatc cctcccaccc ggcggtagac
      901 ctccgcggtc accgccagcg acgcgccgct gaactgccag tgctcgctgg tcgagcccgg
      961 ggggccgggg tcggccagga cgagctggtg gcgacggtgc tggcggcgca ggcggtcgac
     1021 cacgacgtcg accggcagcg ccgccatctc ggcgggcgcg atctcgatcc ggccgccgat
     1081 ggcccgcgcg cccgccgcgg ccgcacgcag ctgggcggcg atccagtcgg gggcgaccgt
     1141 cgagtccgcg tcggtgcacg cgatcaggcc ctcggcgggc agccgcgcgg cggcggcgtc
     1201 catgcccacg cggcgggcgg ggcccgcccc gcggccgggg ccgtcgagca ccgtgacggc
     1261 cagcccggga ttgcgcaccg tggccctcgc cacctccccc gcggtgtcgt cgccgcacgc
     1321 gtccaggacc acgatgacct cccacgcggg cgccggcacg ccgcgctgtg cggccagggc
     1381 gtccaggcag gcgccgatca ggtcctcctc gtcgcgggcg gggaccacga cgcagcagcg
     1441 caggcaggag ctcacggcgt gggcgccccg gactccacga gctcgcgccc gcgctcgtag
     1501 tcgtcgtcgc ggcggtggca gagcacgccc acgcaggtgc cgtcgtcgtc gccgtaccag
     1561 gcggtgaagc cgccgtcgtc gcccgcgtcc aggcggaccg aggagtagcc gtcgccccag
     1621 gccacgtact tgagcgtgcg gtcggcgatc gcgctccaga agccgggggc gttggcccac
     1681 gcgcggtcct cgccggccag cacggctccc gccaccgcac cctggtgcag cgcctcgccc
     1741 cagtgctcga cgggcagcgg ccggcccgcc gccgcgttgt gcgcgtaggc cacgtcgccg
     1801 acggccagga cggcggggtc gctggtgcgc atgtcggccc cggtggcgac gccgccgccc
     1861 tcgacgcgca ggccggcctc ccgggcgagc ccgacccgtg gggccacccc cacggccatg
     1921 aggacggtgt ccgcgtccag ctcggcgtga tcgccggcct gtacgcgcca cgtggagccg
     1981 tcgcgctcga tccggctgag ctccgtgccg gccatcagct cgaccccggc gtcctccatc
     2041 cacgtggtga tccgcccgcc cacctcggga ccgagccgct cggactgcgg cagcgcctcg
     2101 gccgtgacca cggtcaccgc cgcgccgcgc gcggccagcg aggcgcccgc ctcgcagccc
     2161 acgaagcccg agccgacgac gaccacccgg cggccgtgcc gctggagcgc ccgcgcgtcc
     2221 tcgatggtgc gcatcgtcag gacgccgggg tcgtccgccc ccggcacggg aaggcgctgg
     2281 ggctccgatc cggtggccag cacgcagtgc tcgtagtcca ggtgctcacc gtcgtccagc
     2341 gccacctgcc gtcccgccag gtcgagcgcg gtggcccgca ccccggtgcg caggtcgacc
     2401 ttgtgccggg cgaaccacag ctcgtcctcg atcggcaggt cgtgctcggc cgtctcgccg
     2461 cgcaggaact ccttggtcag cggcggccgc tcgtacggcg cgtggggctc ggcggcaacg
     2521 atcgtgaccg gaccgtcgcc tccggcttcg cggaacgcgc gtgccgtggc cagcccggcc
     2581 ggcccgccgc cgacgatgac caccccgcgc ccggtgctca cgcgcgtcta aacaggtcga
     2641 ggcggtagtg ggacccgtcg tggccgtgga ccggcgccag gtcggggcgc cgcgccagcg
     2701 cgtcgtgcac ctgatggccc tgcaacgggt agtcgcgggt gggctcggtc cagtgcaccg
     2761 ccagcagcac gccctcgggc tccagcgccc cggccagcgt gtccagcccg tgctccagca
     2821 gccgctcgtc ccagtagtag aggacctccg agcacacgat caggtcgaag cgcccgggcg
     2881 gcatgtcctc cgggagcgtg gcctgccgga cgctgacgtt gtccagccgg gcggtccgct
     2941 cgcgcgcggc ggccacggcc gcctcggaca cgtcgaccgc caccagctcg cgcgcgacgc
     3001 ccgcgagcgc ctgcgtgaac acgccgatcg agcagccgac ctccagcgcg cgg
//
LOCUS       DASUPW010000551         2601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_405538, whole genome shotgun sequence.
ACCESSION   DASUPW010000551 DASUPW010000000
VERSION     DASUPW010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405538"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..>2601)
                     /locus_tag="VFY44_12435"
     CDS             complement(136..>2601)
                     /locus_tag="VFY44_12435"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5911293.1"
                     /translation="HALHLVATGLAQDRPRLLVVDDAHWGDASSLRALSYLAGRLATA
                     PLVLAISFRSAEPGAPEDLLDQLRAEPGVRTLTPTPLGPGSVATLVRRRLPTADDDAC
                     RAAADVTAGNPLYVTELLRAVAQEGNGSGEAASIQRAALPSLGDRVSRRIARVAEDAP
                     ALAAAMAVLGDGSSLAMAGELAGVSPEVAGRIAHSLRRIEILQSEDPVVFVHPLVRSS
                     VYDSLTTAERDAHHAAAAQLREAARAAPAVVAAHRAAVRPAGSAEAAHTMARAAADAM
                     ARGAPDEAVRWYRRALEEEAREPPRAELLAALGTSEVALLDPAALDHLGQALETSTDP
                     ALSARVAVTLAPVLFAAGQWTAAADVVTAADEMLGDSDPSASAELAGIGLLITGYSLA
                     LVDRVPITPERLESLSSGSGWTVHALAAMRAAYAVHTGETAKGRELVDRALEGGTLLA
                     ERERGIWATSHLLVAMAELDDSERGVAVALEIEAAAEREGSMNSHISAVSHRAWIQAR
                     QGDLTGAVEALEPLITAAIETGNPTFVAAQLFYLQDALLERSGLDDLVAIARSIDLDA
                     VGLAGTWMGAMVLTVRGRLGAAELDTSAAVTDLRAAFAIARGLSMGPAVAPLGSLLAL
                     ALPAEQSDEAARLVAEELEQARATGLARPQGIALRAAGLLAPDQEGVPMLRESIALLD
                     TCGARVERARSLLALGSVLRRSGSRLDAREELTAAVELARTCGADLLAERARDELRAA
                     GGRPRRGAATGPAALTASELRVARLAADGATTPEIARALVVSAKTVETHLTHAYAKLG
                     LSGAGARRRLAEALDGDRAADAD"
BASE COUNT          319 a         1016 c          977 g          289 t
ORIGIN      
        1 tagccaagcg ccccaccgcc ttccggtcgt tccccaagtg caaggtcagc aagaagaaga
       61 tcaactgcgg caagtcgaaa accaaggtgc gggccaaggc gaagaagaag tcccgccgca
      121 agcgctgacc gggaactagt cggcgtccgc cgcccggtcg ccgtccagcg cctcggccag
      181 gcgccggcgg gcgccggcgc ccgagaggcc gagcttggcg taggcgtgcg tcagatgcgt
      241 ctcgacggtc ttcgcgctga ccaccagggc acgggcgatc tcgggcgtgg tggccccgtc
      301 ggcggccagc cgcgcgaccc gtagctcgct ggcggtcaat gccgccggcc cggttgccgc
      361 cccgcgccgg ggccgtccgc cggccgcacg cagctcgtcg cgggcgcgct cggccaggag
      421 gtccgccccg caggtgcgtg ccagctccac cgcggcggtc agctcctccc gggcgtccag
      481 gcgcgagccg gagcgccgca ggacgctgcc cagcgccagg agcgagcggg cgcgctccac
      541 gcgggcgccg caggtgtcca gcagggcaat cgactcgcgc agcatcggca cgccctcctg
      601 gtcgggggcc agcaggccgg cggcgcgcag ggcgatcccc tggggccggg ccaggccggt
      661 ggcgcgcgcc tgctccagct cctcggccac caagcgggcc gcctcgtccg actgctcggc
      721 gggcagggcc agcgccagca gcgatcccag cggggccacg gcggggccca tcgacaggcc
      781 gcgtgcgatg gcgaacgccg cgcgcaggtc ggtcacggcc gcggacgtgt ccagctcggc
      841 ggcgccgagg cgcccccgca cggtgagcac catcgcgccc atccaggtgc ccgccaagcc
      901 cacggcgtcc aggtcgatgg acctggcgat ggcgaccagg tcgtccaggc cgctccgctc
      961 cagcaacgcg tcctggaggt agaagagctg ggcggccacg aacgtcgggt tccccgtctc
     1021 gatcgccgcc gtgatcagcg gctcgagcgc ctccacggcg cccgtcaggt cgccttggcg
     1081 cgcctggatc caggcccggt ggctgaccgc ggagatgtgg ctgttcatcg agccctcgcg
     1141 ttcggccgcg gcctcgatct ccagcgcgac ggccacgccc cgctcgctgt cgtccagctc
     1201 ggccatggcc acgagcaggt gggaggtggc ccagatgccg cgctcgcgct cggccagcag
     1261 ggtcccgccc tccaatgccc tgtccaccag ctcgcggccc ttggcggtct ccccggtgtg
     1321 cacggcgtag gcggcgcgca tggccgccag ggcgtgcacg gtccagccac tgccgctgct
     1381 cagcgactcc agccgctcgg gggtgatcgg cacccggtcc accagggcca gcgagtagcc
     1441 ggtgatcagc agcccgattc cggccagctc cgccgaggcg gaggggtcgc tgtcgcccag
     1501 catctcgtcc gcggcggtga ccacgtcggc ggcggcggtc cactggccgg cggcgaagag
     1561 caccggcgcc agcgtcacgg ccacccgtgc gctcagggcg gggtcggtcg aggtctcgag
     1621 cgcctggccc aggtggtcca gcgcggcagg gtccagcagc gccacctcgg acgtgcccag
     1681 ggcggccagc agctcggccc gcggcggctc ccgcgcctcc tcctccagcg cccgccggta
     1741 ccaccgcacc gcctcgtcgg gcgcgccccg ggccatggcg tcggcggcgg cacgggccat
     1801 cgtgtgggcg gcctcggccg acccggccgg acgcaccgcg gcccggtgcg cggcgacgac
     1861 cgccggcgcc gcgcgcgcgg cctcccgcag ctgcgccgcc gcggcgtggt gggcatcgcg
     1921 ctcggcggtg gtgagcgagt cgtagaccga gctacggacc agcgggtgca cgaacaccac
     1981 cgggtcctcc gattgcagga tctcgatccg gcgcaggctg tgggcgatcc ggcccgcgac
     2041 ctccggcgac acgcccgcca gctcgccggc catggccagc gagctgccgt cgcccaggac
     2101 ggccatcgcc gccgccagcg cgggggcgtc ctcggccacg cgcgcgatgc gccgcgacac
     2161 gcggtcgccg agcgagggca gcgccgcccg ctggatcgac gccgcctcgc cgctgccgtt
     2221 gccctcctgg gccacggcgc ggagcagctc ggtcacgtac agcgggttcc cggcggtcac
     2281 gtcggcggcc gcccggcagg cgtcgtcgtc cgcggtgggc agtcgccggc gcaccagggt
     2341 ggcgaccgat cccggcccga gcggggtggg ggtcagcgtc cggacgcccg gctcggcgcg
     2401 gagctggtcg agcaggtcct cgggggcgcc cggctcggcg gaacggaacg agatggccag
     2461 caccagcggt gccgtggcca ggcgccccgc caggtagctc agggcgcgca gggacgacgc
     2521 gtcgccccag tgcgcatcgt ccaccaccag cagccgcggc cggtcctgcg ccagccccgt
     2581 ggccaccagg tgcagcgcgt g
//
LOCUS       DASUPW010000552         2466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1373466, whole genome shotgun sequence.
ACCESSION   DASUPW010000552 DASUPW010000000
VERSION     DASUPW010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373466"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VFY44_12440"
     CDS             <1..540
                     /locus_tag="VFY44_12440"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HEX5911294.1"
                     /translation="GNWSGPVGGTRGQQPFREGLVVREAQDELRGFTPHQGLIEVECR
                     AAIGPGTMFSAWMVGMEDEPGRCGEICLVEVFGDSLRDDGTAKVGCGIHPFRDPALTE
                     EFAAERRAIDVSELHTYAVEWGPDGVDFLHDGQLMRSSRQSPGYPMMLILGVFDFPGR
                     SPEGFVPELTVTSVIGPAG"
     gene            570..2225
                     /locus_tag="VFY44_12445"
     CDS             570..2225
                     /locus_tag="VFY44_12445"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX5911295.1"
                     /translation="MEAASTPNTRARTLPPWPDQRPLRAWQEAALAKLLESPSGDFLA
                     SATPAAGKTTFGLRVAYELLRRGVVTRVCVVAPTTHICRQWAADAARYGLDLDPGRAN
                     ADGPEPADRHGVAVTYATVASGPQVHAAAAARRRTLVIADEPHHMGELAAWGQSASHA
                     FDRAAFRLLHSGTPFRSDNTAIPWVAYDDDGVSAADHAYTYTDALVDGVCRPITFLAY
                     GGEMEWVSDGKTRTAEFETLLPRQESARRLRTALDANGDWIAQVLRDADERLEEVRAD
                     GHPTAGGLVVAADKEHANHLADRLSRITGRRPPVVTSDDPDASDGIDRFAASAERWLV
                     SVLMVSEGVDIPRLRVGVYATPARTELFFRQVVGRFVRRTPQPRRQMSYLLMPADPDL
                     KLLAAQVEEERRHALELDEALEERDEPVERAQAGDGFTALSSNAHLEDVISSTTAPGD
                     ALLLFADEDSPSMATSAPVGATPTEPEEVVAEPREEAAFQKRRRLRDERAALVSKLAR
                     RGGSDHRSVNAWVNRAVGVTSVADATADQLQRSITVLETELRR"
     gene            complement(2235..>2466)
                     /locus_tag="VFY44_12450"
     CDS             complement(2235..>2466)
                     /locus_tag="VFY44_12450"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5911296.1"
                     /translation="TSLRAAGARPRRPVLRGPGALTPAERRVAELAAAGLSNRDIAER
                     LFVTVRTVEYHLANAYKKLGIDGREKLTAALG"
BASE COUNT          316 a          929 c          913 g          308 t
ORIGIN      
        1 ggcaattggt ccggcccggt gggaggcaca cgtggccagc agccgttccg cgagggcctg
       61 gtggtacgcg aggcccagga cgagctgcgg ggcttcacgc cccaccaggg gctgatcgag
      121 gtcgagtgcc gggccgccat cggccccggg acgatgttct cggcctggat ggtcggcatg
      181 gaggacgagc ccgggcgctg cggtgagatc tgcctggtcg aggtgttcgg cgactcgctc
      241 cgcgacgacg gcacggccaa ggtggggtgc gggatccatc cgttccgcga ccccgcgctg
      301 accgaggagt tcgccgccga gcgccgcgcc atcgacgtct ccgagctcca cacctatgcg
      361 gtcgagtggg gcccggacgg cgtcgacttc ctgcacgacg gccagctgat gcgctccagc
      421 cgccagtccc cgggctaccc gatgatgctg atcctgggcg tgttcgactt cccgggccgc
      481 agccccgagg ggttcgtgcc ggagctgacg gtgaccagcg tcatcggccc cgcgggttga
      541 ccggccccac cactagggtg cccgcttcca tggaggccgc cagcaccccc aacacccgag
      601 cacggacgct cccgccctgg cccgaccagc ggccgctgcg tgcctggcag gaggccgccc
      661 tggccaagct gctggagtcg ccctccggcg acttcctggc ctcggccacc cccgccgcgg
      721 gcaagaccac cttcggcctg cgggtggcct acgaactgct gcggcgcggt gtggtgaccc
      781 gggtgtgcgt ggtggcgccc acgacccaca tctgccgcca gtgggcggcc gacgccgccc
      841 gctacgggct ggacctggat cccgggcggg ccaacgccga cgggcccgag ccggccgacc
      901 ggcacggcgt cgcggtgacc tacgccacgg tggcgtcggg gccgcaggtg cacgccgccg
      961 cggcggcccg gcggcgcacg ctggtcatcg ccgacgagcc gcaccacatg ggcgagctgg
     1021 ccgcgtgggg ccagagcgcg tcgcacgcct tcgaccgcgc cgccttccgg ctgctgcact
     1081 cgggcacccc gttccgctcg gacaacacgg cgatcccgtg ggtggcctac gacgacgacg
     1141 gggtcagcgc ggccgaccac gcctacacct acaccgacgc cctcgtggac ggcgtctgcc
     1201 gcccgatcac gttcctggcc tacggcggcg agatggagtg ggtctccgac ggcaagaccc
     1261 gcaccgccga gttcgagacg ctgctgccgc gccaggagtc ggcgcggcgg ctgcggacgg
     1321 cgctggacgc gaacggggac tggatcgccc aggtgctgcg cgacgccgac gagcggctgg
     1381 aggaggtccg cgccgacggc caccccacgg ccggcgggct ggtggtggcg gccgacaagg
     1441 agcacgccaa ccacctggcc gaccggctgt cgcggatcac cggccgccgg ccgccggtcg
     1501 tgacgtcgga cgaccccgac gcctccgacg gcatcgaccg cttcgccgcc tcggccgagc
     1561 gctggctggt ctcggtgctg atggtcagcg agggcgtgga catcccgcgc ctgcgcgtgg
     1621 gcgtgtacgc caccccggcc cgcaccgagc tgttcttccg ccaggtcgtg ggccggttcg
     1681 tgcgccgcac gccccagccc cggcgccaga tgagctacct gctgatgccg gccgaccccg
     1741 acctcaagct gctggccgcc caggtggagg aggagcgccg ccacgcgctg gagctggacg
     1801 aggcgctgga ggagcgcgac gagccggtcg agcgcgccca ggccggcgac ggcttcaccg
     1861 cgctgtcctc gaacgcccac ctggaggacg tgatctcgtc caccaccgcg cccggcgacg
     1921 cgctgttgct gttcgccgac gaggacagcc cgtcgatggc cacgtccgcg ccggtcggcg
     1981 ccacccccac cgagcccgag gaggtggtgg ccgagccgcg cgaggaggcg gcgttccaga
     2041 agcgccgccg gcttcgcgac gagcgcgccg cgctggtgtc caagctggcc cgccgcggcg
     2101 gcagcgacca ccggtcggtg aacgcgtggg tcaaccgcgc ggtgggcgtg acgtcggtgg
     2161 ccgacgccac cgcggaccag ctccagcgct cgatcaccgt gctggagacc gagttgcggc
     2221 gctgacggag ccggtcagcc cagcgcggcg gtgagcttct cccgcccgtc gatgcccagc
     2281 ttcttgtagg cgttcgccag gtggtactcg acggtgcgca ccgtcacgaa cagccgctcg
     2341 gcgatgtcgc ggttggacag cccggcggcg gccagctcgg ccacgcgccg ttcggccgga
     2401 gtcagagcac ccggtccgcg caggacgggc cgccgcggcc gcgctcccgc ggcgcggagg
     2461 ctggtc
//
LOCUS       DASUPW010000553         3725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_405867, whole genome shotgun sequence.
ACCESSION   DASUPW010000553 DASUPW010000000
VERSION     DASUPW010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405867"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1327
                     /locus_tag="VFY44_12455"
     CDS             68..1327
                     /locus_tag="VFY44_12455"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5911297.1"
                     /translation="MGSPLARIIDEGLITPAYQPLVDLYSGHVLGYEALARGPRGGEL
                     ERPDLLFAAARREGRLADLDRVCRHQAVAQARAAGLRAPTTLFVNVEPEAIGLGEHDE
                     AADLPDDMRLVAEITERALTSRPADLLHMVERVREMGIGIALDDVGADRRSLALMPFL
                     RPDVVKLDLRLVQDRPSKQVAEVHNAVSAHAERTGALVVAEGIETEEHLERARAMGAS
                     VGQGWLFGRPGPLPADTACADRPVPISQRTAGEAADTPFNMIDGQRPQRRGTKRLLLE
                     ISRELERQAELLGDSAVLISTFQHARHFTPIQAELYGALSRQVAFIGALGQDMSGAPA
                     PGVRGANLTSDDPLGAEWDIVVVSPHFAAAFTARDMGDSGADMDRRFDFQLTHDRDLA
                     VRAARILMARIEPLPIEAPASAVSVAA"
     gene            complement(1324..1614)
                     /locus_tag="VFY44_12460"
     CDS             complement(1324..1614)
                     /locus_tag="VFY44_12460"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5911298.1"
                     /translation="MKLYVCYGFLKNVTTTGRPGGHPCGNALKALEAAGHDPEVQAVH
                     GLGVPLLDRTSGRQEVKRLTGQTVVPVLVTDDGEKVFDSKRIVAWAKANPAA"
     gene            complement(1633..2655)
                     /locus_tag="VFY44_12465"
     CDS             complement(1633..2655)
                     /locus_tag="VFY44_12465"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5911299.1"
                     /translation="MSVAVHDVHKSFGATRAVAGVDLELSGGELVSVLGPSGCGKTTL
                     LRMIAGFETPDAGTIVVGGRTVAGPGEWLPPEKRRIGMVFQDYALFPHLRVRDNVGFG
                     LIGRDSAERDRLTARTLELVGLQHKGGSYPHELSGGERQRVALARALAPEPELVLLDE
                     PFSSLDATLRADLRREVALILREAGATALLVTHDQEEALTLGDRLAVMRAGVLVQAGA
                     PAEVYARPADRWTAQFVGEVNVLAGVADGDEVQTEVGRLTLPDPATGPVHVALRPEQL
                     RLDAGRDPNAEVVDRDFRGHDVLYRLRHQTGLTLLAQLPSLELHEVGAQVFVGPVASA
                     VAPVVD"
     gene            complement(2828..>3725)
                     /locus_tag="VFY44_12470"
     CDS             complement(2828..>3725)
                     /locus_tag="VFY44_12470"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5911300.1"
                     /translation="AALQLGTLLVSVTLTLLVVERASRGRARYASEGGRRFTPIRLRG
                     PAAVAAVVAPLALVALVVLAPVVQLTVWSVQSVSDGLLAPEFGTAARNSLMLAVMSIA
                     LVVGAALVMAYGRRVSRSASAGRAVRVATIGYGLPGSVVAVAVLVPLGWIDHRLDDVA
                     GVGLILTGTVISLFVAYTVRFVALAFQSVDAGLERVPRSLDEATRGLGSDRLDVLARI
                     HVPLVWGAMATAGLLVFTEVMRELPATLLLRPLGGDTLAVAVWQATTESLYKTAALPA
                     LLIVLVGLGPVAVLIHLSRRMA"
BASE COUNT          532 a         1439 c         1318 g          436 t
ORIGIN      
        1 ccaccggcgg cgacggttcc tggcccgaat agctaaagcg acacgcagga gtaccgatac
       61 gtgacgggtg gggtcaccgc tcgcacgcat aatcgatgaa ggcctgatca cgccggcgta
      121 ccagccgctg gtcgacctgt acagcggcca cgtgctcggc tacgaggccc tcgcccgcgg
      181 gccccgcggc ggcgagctcg agcggcccga cctgctgttc gccgccgccc gccgcgaggg
      241 ccggctggcc gatctggacc gcgtctgccg gcaccaggcg gtggcccagg cgcgtgccgc
      301 cgggctcagg gcgcccacga cgctgttcgt gaacgtggag cccgaggcca tcggcctggg
      361 cgagcacgac gaggccgccg atctgccgga cgacatgcgg ttggtggccg agatcaccga
      421 gcgcgccctg acctcccgcc ccgccgacct gctgcacatg gtcgagcgcg tgcgcgagat
      481 gggcatcggg atcgccctgg acgacgtcgg cgccgaccgc cgctccctgg cgctgatgcc
      541 cttcctgcgc cccgacgtgg tcaagctcga cctgcggttg gtccaggacc gtccttccaa
      601 gcaggttgcc gaggtgcaca acgcggtcag cgcccacgcc gagcgcacgg gcgcgctggt
      661 ggtggccgag ggcatcgaga ccgaggagca cctggagcgc gcccgcgcca tgggcgcgtc
      721 ggtcggccag ggctggctgt tcggccgtcc cggcccgctg ccggccgaca ccgcctgcgc
      781 cgaccggccc gtgccgatct cccagcggac ggccggcgag gccgcggaca cccccttcaa
      841 catgatcgac ggccagcggc cccagcgccg ggggaccaag cggctgctgc tggagatctc
      901 gcgcgagctc gagcgccagg ccgagctgct gggcgacagc gcggtcctga tcagcacctt
      961 ccagcacgcg cgccacttca cgccgatcca ggccgagctc tacggcgccc tgtcgcgcca
     1021 ggtcgcgttc atcggcgccc tgggccagga catgtcgggc gccccggcgc ccggggtgcg
     1081 cggcgccaac ctgacctccg acgatccgct cggcgcggag tgggacatcg tcgtggtctc
     1141 accgcacttc gcggccgcct tcaccgcccg cgacatgggc gacagcggcg cggacatgga
     1201 ccgccgcttc gacttccagc tgacgcacga ccgcgacctg gcggtgcgcg cggcgcgcat
     1261 cctgatggcg cggatcgagc cgcttccgat cgaagcgccg gcgtccgcgg tctcggtcgc
     1321 ggcctaggcc gccgggttcg ccttcgccca ggcgacgatc ctcttcgagt cgaagacctt
     1381 ctcgccatcg tcggtgacca gcacgggcac caccgtctgg ccggtcagcc gcttgacctc
     1441 ctggcggccg ctggtgcggt ccagcagcgg cacccccagg ccgtggacgg cctgcacctc
     1501 cgggtcgtga cccgcggcct ccagcgcctt cagcgcgttg ccgcacggat ggccgccggg
     1561 gcgccctgtg gtcgtcacgt tcttcaggaa cccgtaacag acgtagagct tcatgggccg
     1621 agcctaatgc ggtcagtcga ccacgggcgc caccgcggag gcgaccgggc ccacgaacac
     1681 ctgggcgccc acctcgtgca gctccagcga cggcagctgc gccagcagcg tcaggccggt
     1741 ctggtggcgt aggcggtaga gcacgtcgtg gccgcggaag tcccggtcca ccacctcggc
     1801 gttggggtcg cgcccggcgt ccagtcgcag ctgctcgggc cgcagcgcca cgtgcaccgg
     1861 gccggtggcg ggatccggca gcgtcagccg gccgacctcg gtctgcacct cgtccccgtc
     1921 ggccacgccg gcaagcacgt tcacctcgcc gacgaactgg gccgtccacc ggtccgcggg
     1981 ccgcgcgtac acctcggcgg gggcgccggc ctgcaccagg acgccggcgc gcatcacggc
     2041 caggcggtcg cccagcgtca gcgcctcctc ctggtcgtgg gtgaccagca gcgcggtggc
     2101 gcccgcctcg cgcaggatca gcgcgacctc gcggcgcaag tcggcgcgca gggtggcgtc
     2161 caggctggag aacggctcgt ccagcagcac cagctccggc tcgggcgcca gcgcccgcgc
     2221 cagcgccacc cgctggcgct cgccccccga cagctcgtgc gggtagctgc cgcccttgtg
     2281 ctggagcccg accagctcga gcgtccgggc cgtcagccgg tcgcgctcgg ccgagtcgcg
     2341 gccgatcagg ccgaagccca cgttgtcgcg cacgcgcagg tgcgggaaca gcgcgtagtc
     2401 ctggaagacc atcccgatcc ggcgcttctc gggcgggagc cactcgccgg ggccggccac
     2461 cgtccggccg ccgacgacga tggtgccggc gtcgggggtc tcgaagccgg cgatcatgcg
     2521 cagcaacgtc gtcttgccac agcccgacgg gccgagcacc gagacgagct cgccaccgga
     2581 gagctccagg tccacgccgg cgacggcgcg ggtggcgccg aaggacttgt ggacgtcgtg
     2641 gacggcgacg ctcacgggcc gaccccccgg acacgcgtca cgactttcgt ctgcatacga
     2701 gacggaactc gtgacgctgc gggtgccggg gctccagccg cctccgagtg caacggcacc
     2761 gttacaggcc gccctctacc cagccggccg tcggccgagc ccgcccgagt cgatgccggg
     2821 agaacgctca cgccatcctc cgcgacaggt ggatcagcac ggccaccggg cccaggccca
     2881 ccagcacgat cagcagggcg ggcagcgcgg ccgtcttgta gagcgactcg gtcgtggcct
     2941 gccagacggc cacggccagc gtgtcgccgc ccagcgggcg gagcagcaac gttgcgggca
     3001 gctcgcgcat gacctcggtg aataccagca ggcccgcggt ggccatggcg ccccagacca
     3061 gcggcacgtg gatgcgcgcc aggacgtcca ggcggtcgct gcccaggccg cgggtcgcct
     3121 cgtccagcga gcgtgggacg cgctccaggc cggcgtcgac cgactggaag gccagcgcca
     3181 cgaagcgcac cgtgtaggcc acgaacaggc tgatcacggt gccggtcagg atcaggccga
     3241 caccggccac gtcgtccagg cggtggtcga tccagcccag cggcaccagc accgcgaccg
     3301 ccaccaccga gcccggcagc ccgtagccga tggtggccac gcgcaccgcg cggccggccg
     3361 aggccgagcg cgagacccgg cgcccgtagg ccatgaccag cgctgcgccc accacgagcg
     3421 cgatcgacat gaccgccagc atcaggctgt tccgcgccgc ggtgccgaac tcgggtgcca
     3481 gcagcccatc cgagaccgac tgcaccgacc acacggtcag ctggaccacc ggcgccagga
     3541 ccaccagcgc caccagcgcc agcggggcga ccacggcggc cacggcggcc ggcccacgga
     3601 gccggatcgg cgtgaagcgc cggccaccct cggaggcgta gcgggcccgc ccgcgcgagg
     3661 cgcgctcgac caccagcagc gtcagcgtga ccgacaccag cagcgtgccc agctgcagcg
     3721 ccgcg
//
LOCUS       DASUPW010000554         5733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_681102, whole genome shotgun sequence.
ACCESSION   DASUPW010000554 DASUPW010000000
VERSION     DASUPW010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5733
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681102"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VFY44_12475"
     CDS             complement(<1..653)
                     /locus_tag="VFY44_12475"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX5911301.1"
                     /translation="MTALDWGIIAFVLLMALWGYMQGLIVGFLSLAGFAGGAFIGSRV
                     APKLLEEGSSSPYAPVFALVGALLAGGILASGLELLGFRLRHRLNESLGVVDGMGGAL
                     LVACLGLGLVWVAGAVALHTPGAPGLREPIQRSSVLTRLNEMLPPSGPLLQALARFDP
                     FPTITGPDPGVSAPDSAIARDPEVRAAGRGVVRVLGTACGLGVQGSGWVASDGVVVT"
     gene            754..2361
                     /locus_tag="VFY44_12480"
     CDS             754..2361
                     /locus_tag="VFY44_12480"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX5911302.1"
                     /translation="MAVGAMGIGLAVGSGRESEAERSAKQFAAAWNREDYRSMHALLD
                     EESRRRYPLATFRRAYEQAAGTATATAARVGEPRGAGGGRQRIPVVVATRIFGEVRGQ
                     VSLPVDEAGVAWSPELAFPGLAEGERLSRQTQAPLRATIVSVDNKVLAEGPATGRSSP
                     EGVGSSIAGTVEPSDDRAVQDAVYARGFPRDTPVGTSGLERALQSQVEGFPGGTLVAG
                     RRVLGRAKARPAPRVRSTIDSRLQEAAVEALAGRLGGIAALEPKTAEVRALAGIAFSA
                     PQPPGSTFKVITTAAALEKKKVKVGDRFPVETKAVIDGVDLENANGESCGGNFAESFA
                     HSCNSVFAPLGVKVGAAELVSMAERFGWNKPPSLAGAQMSTIPKAGDIKSPLDLGSSA
                     IGQGRVLATPLEMASVAQTVANDGVRVPPTLQPGVRRAPKRVISRPIADTIEGLMIGV
                     VRYGTGTASQIPGVAVAGKTGTAELGDTRGPDAKSGPENTDAWFTAYAPAKRPKLAVG
                     VLFVRNGAGGATAAPAARIVLGRALGK"
     gene            complement(2444..3037)
                     /locus_tag="VFY44_12485"
     CDS             complement(2444..3037)
                     /locus_tag="VFY44_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911303.1"
                     /translation="MALIRVPRMVAACAAALALAGGLGACGFEKPELGTEEEPGREGL
                     ALELAGVDYNLFITRELNLRITPDKAYYKGPAAAPGKVLYGVFVKVCGAKKGERVTSV
                     PVRNFIVKDNQGNEYHPKELPEDDAFAYQPRPLSFEECIPQSGSVAQQGPTAGSMLLY
                     EFPLQNTENRPLEMVIKGPVEDLSDLKRESLHIELDL"
     gene            complement(3051..3611)
                     /locus_tag="VFY44_12490"
     CDS             complement(3051..3611)
                     /locus_tag="VFY44_12490"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5911304.1"
                     /translation="MRQVQVETVISVPREQVFDFVGDLSLRPSFADHYLKDFRLTGAN
                     PVGKGAGARFLLDTPVFSERGGLVITEYDRPRRIVEEGGVGRRGRSRLVAVYDFSPEA
                     GGGTRVELTTFSEPGNWVDRIKQTGVHRWVRRQSKKALKRLKGQLEEPGRKPLKRVTV
                     AGYESLKAPRFGAHVPVEHQTAPADG"
     gene            complement(3698..3940)
                     /locus_tag="VFY44_12495"
     CDS             complement(3698..3940)
                     /locus_tag="VFY44_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911305.1"
                     /translation="MPDLAGLLLRKSVGSLDSGRARCSKCHRTPLTGERLHETDGGRR
                     FCELCLEFVPERDRATIRIERVHATDRHISAAPRAA"
     gene            4005..4778
                     /locus_tag="VFY44_12500"
     CDS             4005..4778
                     /locus_tag="VFY44_12500"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX5911306.1"
                     /translation="MATTVTILSQKGGTGKTTTTRTLTDIFRRVGLSVLAVDLDPQGN
                     LSDYFDIPADAAPTVADVLTGQAKAMDAIHDDVLPANLGLAEAELVLGGKMGREMTLR
                     RALREPGKHYDLILVDCPPSLGLLTVNALVAADRALITSEAEYFSLQGVEQALEVVEL
                     AKDSLNPDLEWLGVVLNIADMRLVHSREALKQLQERFGDKVFEAVIRRSVAYAESAER
                     GVSIVDRRPDLAADYMDLAEEALNRLDLDEEHSSVAALR"
     gene            4794..>5733
                     /locus_tag="VFY44_12505"
     CDS             4794..>5733
                     /locus_tag="VFY44_12505"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX5911307.1"
                     /translation="MPATIADLLRKPALELAEMVRSGEVSARELTQASLDRIEATEPL
                     NHWTLVDAEGALAAADAIGPGDDRPFAGVPLGLKDLFLPVKGMRLTQGSDITPDFVPD
                     YDSAVARRFRDAGFVFMGRTQSPEFGILPVTEPRRFGPCRNPWDPDRTTGGSSGGSGG
                     AVAAGAVPVAHASDGGGSIRIPAACNGLVGLKSSRGRISRAPDLGDHMLSTDGVLTRT
                     VADSAALLDLLAGYETGDATWASPPEAPFADLAAREPGALRIALAFDLPVESELDPQY
                     EQAARDTAKLLEELGHHVEEVDAPWKGVDLLPTFSVL"
BASE COUNT          805 a         2096 c         2111 g          721 t
ORIGIN      
        1 ttggtgacca ccaccccgtc ggaggccacc cagcccgagc cctggacgcc cagcccgcac
       61 gccgtgccca ggacgcgcac caccccgcgc ccggccgcgc gcacctcggg gtcgcgggcg
      121 atggccgagt ccggcgccga cacgcccggg tccgggccgg tgatggtcgg gaacgggtcg
      181 aaccgggcca gggcctgcag cagcggcccg gacgggggca acatctcgtt caggcgggtc
      241 agcaccgacg agcgctggat cggctcgcgc aggccgggcg ctcccggggt gtgcagcgcc
      301 acggcgcccg cgacccacac caggcccagc ccgaggcacg cgaccaacag ggcgccgccc
      361 atcccgtcca cgacccccag gctctcgttg agccggtggc gcaggcggaa gcccagcagc
      421 tccagccccg aggccaggat gccgcccgcc agcagcgcgc ccaccagggc gaacaccggg
      481 gcgtacggcg aggacgatcc ctcctccagc agcttcggcg ccacgcgcga gccgatgaac
      541 gcgccccccg cgaagcccgc cagcgagagg aagccgacga tgaggccctg catgtagccc
      601 cacagggcca tcagcaggac gaaggcgatt atcccccagt ccagagccgt cacgagggct
      661 tgacggtagc ctgagggtca ggtggcctac ccgaggacga cgaacgagag gcgtaggcgc
      721 ctgacacacc gcgcggtccc cgcactcgtc gtcgtggccg ttggcgcgat gggcatcggc
      781 ctggccgtcg ggtccggccg cgagtccgag gccgagcgca gcgcgaagca gttcgcggcc
      841 gcctggaacc gcgaggacta ccgctcgatg cacgccctgc tggacgagga gtcgcggcgc
      901 cgctacccgc tggccacgtt ccgccgggcc tacgagcagg cggcgggcac cgcgaccgcc
      961 accgccgccc gggtcggcga gccgcgcggg gccggcggcg ggcgccagcg catccccgtc
     1021 gtggtggcca cgaggatctt cggcgaggtg cgcggacagg tctccctgcc ggtcgacgag
     1081 gccggggtgg cctggtcccc cgagctggcc ttcccggggc tggccgaggg cgagcggctc
     1141 agccgccaga cccaggcgcc actccgggcg acgatcgtgt cggtggacaa caaggtgctg
     1201 gccgaaggcc cggcgaccgg gcgctcgtcg cccgagggcg tgggctcgtc gatcgcgggc
     1261 acggtcgagc ccagcgacga ccgcgccgtc caggacgcgg tctacgcccg cggcttcccg
     1321 cgcgacaccc cggtcggcac gagcggcctg gaacgggcgc tgcagagcca ggtcgagggg
     1381 ttcccgggcg gcacgctcgt ggccggtcgc agggtgctgg gccgggccaa ggcccgcccc
     1441 gcgccgcgcg tgcggtcgac catcgacagc cgcctgcagg aggcggccgt cgaggcgctg
     1501 gccggccgcc tgggcggcat cgccgcgctg gaacccaaga cggccgaggt gcgggccctg
     1561 gccggcatcg ccttctcggc gccccagccg cccggctcga cgttcaaggt catcaccacc
     1621 gcggcggcgc tggagaagaa gaaggtcaag gtgggcgacc ggttcccggt cgagaccaag
     1681 gccgtgatcg acggcgtcga cctggagaac gccaacggcg agtcgtgcgg cggcaacttc
     1741 gccgagagct tcgcccactc gtgcaactcg gtgttcgcgc cgctgggcgt caaggtcggc
     1801 gccgccgagc tggtgtccat ggccgagcgc ttcggctgga acaagccgcc ctcgctggcc
     1861 ggggcccaga tgagcacgat cccaaaggcc ggcgacatca agtcgcccct ggacctgggc
     1921 tcctcggcca tcggccaggg ccgggtgctg gccaccccgc tggagatggc gtccgtggcc
     1981 cagacggtgg ccaacgacgg cgtgcgcgtg ccccccaccc tccagcccgg cgtccggcgc
     2041 gcgcccaagc gggtgatctc gcggccgatc gcggacacga tcgaggggct catgatcggc
     2101 gtggtccgct acggcaccgg gaccgcctca cagatacccg gcgtcgcggt ggccggcaag
     2161 accggcacgg ccgagctggg cgacacccgc ggcccggacg ccaagtcggg ccccgagaac
     2221 accgacgcct ggttcaccgc ctacgccccg gccaagcggc cgaagctcgc ggtgggcgtg
     2281 ctcttcgtgc gcaacggggc gggcggggcc acggcggcac cggcggcacg gatcgtgctg
     2341 ggccgcgcgc tggggaagta acggcaggag gctgcggagc agccggtctc ggggccagga
     2401 tggcccctcg gcatcaaggc gcggccaggg atggccgcaa gagctacaaa tccagctcga
     2461 tgtgcagcga ctcccgcttg aggtcgctca ggtcctcgac cgggcccttg atgaccatct
     2521 ccagcgggcg gttctcggtg ttctgcagcg ggaactcgta gagcagcatc gagccggcgg
     2581 tgggcccctg ctgggccacg ctgccggact gggggatgca ctcctcgaag gagagcggac
     2641 gcggctggta ggcgaaggcg tcgtcctcgg gcagttcctt ggggtggtac tcgttgccct
     2701 ggttgtcctt cacgatgaag ttgcgcaccg ggacgctggt gacgcgctcg cccttcttgg
     2761 cgccgcagac cttgacgaag acgccgtaga gcaccttgcc gggcgcggcg gccgggccct
     2821 tgtagtaggc cttgtcgggc gtgatcctga ggttcagctc ccgggtgatg aacaggttgt
     2881 agtccacgcc cgcgagctcg agcgccaggc cctctcggcc cggctcctcc tcggtgccca
     2941 gctcgggctt ctcgaagccg caggcgccca gcccaccggc cagggccagg gcggcggcgc
     3001 acgcggcgac catcctcggg acgcgaatca gggccatcgg ggcgggcagt ctacccgtcg
     3061 gccggagcgg tctggtgctc gacgggaacg tgggcgccga agcggggggc cttgagcgac
     3121 tcgtaaccgg cgacggtgac ccgcttgagc ggcttgcggc cgggctcctc gagctggccc
     3181 ttgagccgct tcagggcctt cttcgactgg cgccggaccc accggtggac gccggtctgc
     3241 ttgatccggt cgacccagtt gccgggctcg ctgaacgtgg tcagctcgac acgggtgccg
     3301 ccgccggcct cgggcgagaa gtcgtagacg gccacgagcc gggaccgccc gcggcggccc
     3361 acgccgccct cctcgacgat ccggcgcggc cggtcgtact cggtgatcac gaggcctccg
     3421 cgctcgctga acaccggggt gtccagcagg aagcgggccc cggcgccctt gccgaccggg
     3481 ttggcgccgg tcagccggaa gtccttcagg tagtggtcgg cgaacgacgg ccgcagcgac
     3541 aggtcgccga cgaagtcgaa gacctgttcg cgcggaacgg agatgaccgt ctcgacctgt
     3601 acctggcgca ctcccgggat attacgtggt gcggtgctcg cgcgaacgac gctcgcgctg
     3661 cgctcctccc ccagtacggg tggcgcggga agggagctca ggcggcgcgc ggcgcggccg
     3721 agatgtgccg gtcggtcgcg tgcacccgct cgatgcggat ggtggcgcga tcgcgctcgg
     3781 gcacgaactc caggcacagc tcgcagaagc ggcgcccgcc gtcggtctcg tgcagccgct
     3841 cgccggtgag cggcgtgcgg tggcacttcg agcagcgggc gcggccgctg tccagcgagc
     3901 cgacgctctt gcggagaagg agaccggcaa gatcgggcat ggaccgactt tcgccgcccc
     3961 cgcccgcgct cctccccgcg agccggccag gtatcgtcgc gcccttggcc accaccgtca
     4021 cgatcctgtc ccagaagggc ggcaccggga agaccacgac cacccggacg ctcacggaca
     4081 tcttccgccg cgtcggcctg tcggtgctgg ccgtggacct ggacccccag ggcaacctct
     4141 cggactactt cgacatcccg gccgacgccg cgcccaccgt ggccgacgtg ctgaccggcc
     4201 aggccaaggc catggacgcg atccacgacg acgtgctgcc ggccaacctc gggctggccg
     4261 aggccgagct ggtcctcggc ggcaagatgg gccgcgagat gaccctgcgg cgcgcgctgc
     4321 gcgagccggg caagcactac gacctgatcc tggtggactg cccgccgtcg ctggggctgc
     4381 tgacggtgaa cgccctggtg gccgccgacc gcgcgctgat caccagcgag gccgagtact
     4441 tcagcctcca gggcgtggag caggcgctcg aggtggtcga gctggccaag gactcgctga
     4501 acccggacct ggaatggctc ggcgtggtgc tgaacatcgc cgacatgcgc ctggtgcact
     4561 cgcgcgaggc gctcaaacag ctgcaggagc ggttcggcga caaggtgttc gaggcggtca
     4621 tccgccgttc ggtggcctac gccgagtcgg ccgagcgcgg ggtctcgatc gtcgaccgcc
     4681 gccccgacct ggcggccgac tacatggacc tggccgagga agcgctgaac cgcctggacc
     4741 tggacgagga gcactcgtcc gtcgccgctc tgcgctgagt taggctcgcg ggcatgcccg
     4801 ccaccatcgc cgacctcctg cgcaagcccg ccctcgaact ggccgagatg gtccgcagcg
     4861 gcgaggtgtc cgcgcgcgag ctgacccagg cgtcgctgga ccggatcgag gccaccgagc
     4921 cgctgaacca ctggacgctg gtcgacgccg agggcgccct ggccgcggcc gacgcgatcg
     4981 ggcccggcga cgaccgtccc ttcgccgggg tcccgctggg gctgaaggac ctgttcctgc
     5041 cggtgaaggg catgcgcctg acccagggca gcgacatcac gcccgacttc gtgcccgact
     5101 acgactcggc cgtggcccgc cgcttccgcg acgccggctt cgtgttcatg ggccggaccc
     5161 agtcgcccga gttcgggatc ctgccggtga ccgagccgcg ccggttcggc ccgtgccgca
     5221 acccgtggga ccccgaccgc accaccggcg gctccagcgg cggcagcggc ggcgcggtgg
     5281 ccgcgggcgc cgtgcccgtc gcacacgcca gcgacggcgg cggctcgatc cgcatccccg
     5341 ccgcgtgcaa cggcctggtg ggcctcaagt ccagcagggg gcggatctcc cgcgctccgg
     5401 acctgggcga ccacatgctg tccacggacg gcgtcctgac ccgcacggtg gccgactcgg
     5461 cggccctgct ggacctgctt gccgggtacg agacgggcga cgccacctgg gcgagcccgc
     5521 cggaggcccc gttcgccgat ctggccgccc gcgagccggg cgcgctgcgg atcgcgctgg
     5581 ccttcgacct gccggtggag tccgagctgg atccccagta cgagcaggcg gcgcgcgaca
     5641 cggcgaagct gctggaggag ctcggccacc acgtggagga ggtggacgcg ccgtggaagg
     5701 gcgtcgacct gctgcccacg ttctcggtgc tgt
//
LOCUS       DASUPW010000555         2558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_2068582, whole genome shotgun sequence.
ACCESSION   DASUPW010000555 DASUPW010000000
VERSION     DASUPW010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2068582"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..2078
                     /locus_tag="VFY44_12510"
     CDS             153..2078
                     /locus_tag="VFY44_12510"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX5911308.1"
                     /translation="MRSFTPDQAVGSSAFPPIADYGFLSDCETTALVAPDGNVEWMCL
                     PRMDSPSIFGSILDRDAGGFKMGPADVSVPAQRRYLPGTMVLETSWGTKGGWIVVRDV
                     LLIGPWHHETERSTTHRRSPTDYDADHVLLRMVRCVNGEVQVNLDCEPVFDYGRKFAT
                     WEYAGTGYHEAIAKAEGSDIELCLTTDMNLGIEGPRATARTMMHEGDTLFAALSWSEH
                     APPKSYDEAYDRMTWTAHHWQHWLDHGEFPDHPWRTFLQRSALTLKGLSYAPTGAIMA
                     AATTSLPEAPGGERNWDYRYMWIRDSTFTLWGLYTLGFDWEANDFFYFVADVAEAEEG
                     QLQIMYGIDGESELVEEELDHLSGYEGARPVRIGNGAYNQNQHDVWGAVLDSFYLHTK
                     SRDHLPERIWPILKKQVEDAIEHWQEPDQGLWEVRGEPKHFTSSKLMCWVALDRGARL
                     AEIREEKELAEEWQKVADEIKDDICENALDDRGVFCQHYDTDTLDASVLLMPLVRFLD
                     PEDERIRATVLAIADELTQDGLVLRYRTDDTDDGLSGEEGTFTICSFWLVSALVEIGE
                     LVRGRELCEKLLSYASSLQLYAEEIDPRSGRHLGNFPQAFTHLALINAVMHVIRADQD
                     LEESSFERELQRTRLVK"
     gene            2075..>2558
                     /locus_tag="VFY44_12515"
     CDS             2075..>2558
                     /locus_tag="VFY44_12515"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5911309.1"
                     /translation="MTEGEKRERGKRLMERLKAADSQPGLLKAAAMARKALPGDDELG
                     GGDEGRESLTREAGLTALQVWQSVSEAQGRGRGEVDVTILFTDLVDFSTWALEAGDEA
                     AIELVGDVDEVCQDAVVDNGGEVVKTMGDGMMAVFGESLEAVRAARAALDGVAKLEHE
                     G"
BASE COUNT          411 a          893 c          907 g          347 t
ORIGIN      
        1 ctgatttccg gtgaagacaa ggcagatgcc gctgcgcgcg cgttcgccga cgcgcccagc
       61 cccgacaccc ccgccgggct cgtgaacccc cggcccggca ccctgaccgt gctgatcgac
      121 gcgcccgccg cggcgcggct gggggccgcc cagtgaggag cttcaccccc gaccaggcgg
      181 tgggctcgag cgcgttcccg ccgatcgccg actacggctt cctgtccgac tgcgagacca
      241 cggcgctggt ggcgcccgac ggcaacgtcg agtggatgtg cctgccgcgg atggactcac
      301 cctcgatctt cggctcgatc ctggaccgcg acgccggcgg gttcaagatg ggccccgccg
      361 acgtcagcgt gcccgcgcag cggcgctacc tgccggggac gatggtgctg gagaccagct
      421 ggggcaccaa gggcggctgg atcgtcgtcc gcgacgtgct gctgatcggc ccctggcacc
      481 acgagaccga gcgctcgacc acgcaccgcc ggtcgcccac cgactacgac gccgaccacg
      541 tgctgctgcg catggtccgg tgcgtgaacg gcgaggtgca ggtgaacctg gactgcgagc
      601 cggtgttcga ctacggccgc aagttcgcca cctgggagta cgccggcacc ggctaccacg
      661 aggccatcgc caaggccgag ggctcggaca tcgagctctg cctgaccacc gacatgaacc
      721 tgggcatcga gggcccgcgg gccaccgcgc ggacgatgat gcacgagggc gacacgctgt
      781 tcgccgccct gtcgtggtcc gagcacgcac cgcccaagag ctacgacgag gcctacgacc
      841 ggatgacgtg gaccgcccac cactggcagc actggctgga ccacggcgag ttccccgacc
      901 atccctggag gacgttcctg cagcgcagcg ccctgacgct gaagggcctg tcctacgcgc
      961 ccacgggggc gatcatggcg gcggccacga cctcgctgcc cgaggccccg ggcggcgagc
     1021 gcaactggga ctaccgctac atgtggatcc gcgactccac gttcaccctg tgggggctgt
     1081 acacgctggg cttcgactgg gaggccaacg acttcttcta cttcgtggcc gacgtggccg
     1141 aggcggagga gggccagctg cagatcatgt acggcatcga cggcgagtcc gagctggtcg
     1201 aggaggagct ggaccatctc agcggctacg agggcgcgcg cccggtgcgg atcggcaacg
     1261 gggcctacaa ccagaaccag cacgacgtgt ggggcgccgt cctggactcc ttctacctgc
     1321 acaccaagtc gcgcgaccac ctgcccgagc gcatctggcc gatcctcaag aagcaggtcg
     1381 aggacgccat cgagcactgg caggagcccg accagggcct gtgggaggtg cgcggcgagc
     1441 ccaagcactt cacctcgtcg aagctcatgt gctgggtcgc gctggaccgt ggcgcacgtc
     1501 tggccgagat ccgcgaggag aaggagctgg ccgaggagtg gcagaaggtc gctgacgaga
     1561 tcaaggacga catctgcgag aacgcgctgg acgaccgcgg cgtgttctgc cagcactacg
     1621 acaccgacac gctggacgca tcggtgctgc tgatgccgct ggtgcgcttc ctcgatcccg
     1681 aggacgagcg catccgcgcc accgtgctgg ccatcgccga cgagctgacc caggacggcc
     1741 tggtcctgcg ctaccgcacc gacgacaccg acgacggcct ctccggcgag gagggcacgt
     1801 tcaccatctg ctcgttctgg ctggtctcgg cgctggtgga gatcggcgag ctggtccgcg
     1861 gccgggagct gtgcgagaag ctcctgtcct acgcctcctc gctccagctc tacgccgagg
     1921 agatcgaccc gcgctcgggc cgccacctgg gcaacttccc gcaggcgttc acccacctgg
     1981 ccctgatcaa cgcggtgatg cacgtgatcc gcgccgacca ggacctggag gagagctcct
     2041 tcgagcgtga gctccagcgc acccgcctgg tcaagtgact gagggcgaga agcgcgagcg
     2101 cgggaagcgc ctgatggagc ggctcaaggc cgccgacagc cagcccggcc tgctcaaggc
     2161 ggccgccatg gcgcgcaagg cgctccccgg cgacgacgag ctgggcgggg gcgacgaggg
     2221 ccgcgagtcg ctgacccgcg aggccggcct gaccgcgctc caggtctggc agtcggtgtc
     2281 cgaggcccag ggccgcggtc gcggcgaggt ggacgtgacg atcctgttca ccgacctggt
     2341 cgacttctcg acctgggccc tggaggccgg cgacgaggcg gccatcgagc tggtcggcga
     2401 cgtcgacgag gtctgccagg acgccgtggt ggacaacggc ggcgaggtgg tcaagaccat
     2461 gggcgacggc atgatggccg tgttcggcga gtccctggag gcggtccgcg ccgcccgcgc
     2521 cgcgctggac ggggtggcga agctcgagca cgagggct
//
LOCUS       DASUPW010000556         3762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_2068674, whole genome shotgun sequence.
ACCESSION   DASUPW010000556 DASUPW010000000
VERSION     DASUPW010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2068674"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VFY44_12520"
     CDS             <1..735
                     /locus_tag="VFY44_12520"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5911310.1"
                     /translation="DFLSARQFHRLELYDEIYRKLGAEDQIAFGLPGDVVVGVAFNRP
                     RRDFTARDRAVLETIRPHMASAFGEARARERATALIAALMSGVQRAGGTVVVLDRANG
                     VQHADEPARHLLEAYAGPGMPPAPVAEWLAGAPGSAPLELAGPNGVLSVSHHRTGDTG
                     ALLLEEHRAGPDPAELRALGLTRREAEVLELVAIGRTNQEIADAMVITPGTVRKHLER
                     IYAKLGVHTRTEAAARAHSRPPHGIA"
     gene            742..2112
                     /locus_tag="VFY44_12525"
     CDS             742..2112
                     /locus_tag="VFY44_12525"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX5911311.1"
                     /translation="MAEALSPSDLSAIQAERGPVLMHVGGICVFEGHVAREAVVQRVR
                     ERVHLIPRYAMRLDEAPLGLANPAWIDDDTFDPDRHVRRVALPAPGGDAELCELVGQL
                     LSEPLDRGRPLWQMTLVEGLAGDRTALVAKMHHALVDGMAAVDVSTVILDPTPEPLDI
                     KPPPRTPASKPGRAARLEGLTRLASAQLALPRSLAREAVTRTVDPRTYASQLKSAASL
                     AGELARVRPQAPETRLNTPIGRDRVFALARCTLEEIKTVRRATGATVNDVLLTVVALM
                     LSRFLGAGAPGHAVALVPVSVRTAEDEGELGNRISTVFVDLPMSGEPLERLERVSAAM
                     DDVKGSAQVRAGALIVGATGLAPPVVSSLAVRAMSGPRMFNLVVSNVPGPQQTLYLDG
                     TPLREVYPAVPLNPRNQALSIGIVSYDGGVGFGLLGDRDALSDIGDAAAGLEATVAEL
                     VHAATG"
     gene            2112..3191
                     /locus_tag="VFY44_12530"
     CDS             2112..3191
                     /locus_tag="VFY44_12530"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX5911312.1"
                     /translation="MATASDVLAFRVERHHLARRVADARVAAGVGLQDTPPGTAALAL
                     AARAEVGPEALDDLVIVPSVRGAPLAVAPEDLPLFTAGLAPPDEDAAKAVIGNAWKEL
                     DGVTAMDALDRVSDAVRDSLASGPLARDDFHQALRDRLPDELLWWCKGCQSHHVHPSL
                     WRATGIRGVLAIVGREGRSAVFGAPPPAPEAVADPGAALARRFLGAYGPARPKLLAEW
                     AGITTAHARALWERAGELAAVEVDGREAWVLAEDATALAEPAPAEGVRLLPNLDPLAG
                     SRDREVLVPDAELRKRVWGRLGGPGLVLVDGAVAGLWRPAKKGRKLVVTVEATGRISR
                     AVKDALAGEAERIAPFRGAESAELA"
     gene            3208..3291
                     /locus_tag="VFY44_12535"
     tRNA            3208..3291
                     /locus_tag="VFY44_12535"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3242..3244,aa:Leu,seq:cag)
     gene            complement(3303..3614)
                     /locus_tag="VFY44_12540"
     CDS             complement(3303..3614)
                     /locus_tag="VFY44_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911313.1"
                     /translation="MSAPGPAYAAFRSALNSGNLDRIRRTARQLPAVRLEDALDICVA
                     YRDEPELYERAAIRWIGRFCLEAKGVGIEDVYQVAEALDRLPDHPESTAAELAKLIGR
                     R"
BASE COUNT          457 a         1418 c         1428 g          459 t
ORIGIN      
        1 gacttcctgt cggcgcgcca gttccaccgc ctcgagctct acgacgagat ctaccgcaag
       61 ctcggcgcgg aggaccagat cgccttcggc ctgcccggcg acgtggtcgt gggcgtcgcc
      121 ttcaaccgcc cccggcgcga cttcaccgcc cgcgaccggg ccgtgctgga gaccatccgg
      181 ccgcacatgg cgtcggcctt cggcgaggcc cgcgcccgcg agcgcgcgac cgcgctgatc
      241 gcggccctga tgagcggggt ccagcgcgcc ggaggcacgg tcgtcgtgct ggaccgcgcc
      301 aacggcgtcc agcacgcgga cgagcccgcc cgccacctgc tggaggcgta cgccgggccc
      361 gggatgccac ccgccccggt ggccgagtgg ctggccgggg cgcccggctc ggcgccgctg
      421 gagctggccg ggcccaacgg ggtactgagc gtcagccacc accgcaccgg cgacaccggc
      481 gccctcctgc tcgaggagca ccgcgccggc ccggaccccg cggagctgcg cgcgctgggg
      541 ctgacgcgcc gcgaggccga ggtcctggag ctggtggcca tcgggcgcac caaccaggag
      601 atcgccgacg cgatggtcat caccccgggc accgtccgca agcacctgga gcgcatctac
      661 gcgaagctcg gggtgcacac ccggacggaa gccgcggcgc gggcgcactc gcggccgccc
      721 cacgggatag cctgacgagc catcgccgag gccctctccc cctccgacct gtccgccatc
      781 caggccgagc gcggtcccgt cctcatgcac gtgggcggca tctgcgtgtt cgagggccac
      841 gtcgcccgcg aggcggtggt gcagcgcgta cgcgagcgcg tgcatctgat cccccgctac
      901 gcgatgcgcc tggacgaggc cccgctgggg ctggccaacc cggcctggat cgacgacgac
      961 accttcgacc ccgaccgcca cgtccggcgc gtggccctcc cggcgccggg cggcgacgcc
     1021 gagctgtgcg agctggtcgg ccagctgctg tccgagccgc tggaccgcgg ccggccgctg
     1081 tggcagatga cgctggtcga gggcctggcc ggcgaccgca cggcgctggt ggccaagatg
     1141 caccatgcgc tggtcgacgg catggccgcg gtggacgtga gcacggtgat cctggacccc
     1201 acgccggagc cgctggacat caagcccccg ccgcgcacgc cggcgtccaa gcccggccgc
     1261 gccgcccggc tggaggggct gacgcggctg gcctcggccc agctggcgct gccgcgcagc
     1321 ctggcccgcg aggccgtgac gcgcacggtc gacccgcgca cctacgccag ccagctcaag
     1381 agcgcggcct cgctggccgg cgagctggcc cgggtccggc cgcaggcgcc cgagacccgc
     1441 ctgaacaccc ccatcgggcg cgaccgcgtg ttcgcgctgg cgcggtgcac gctcgaggag
     1501 atcaagacgg tgcgccgggc caccggcgcc acggtcaacg acgtgctgct gacggtggtc
     1561 gcgctgatgc tctcgcgctt cctgggcgcg ggcgcgcccg gccacgccgt cgcgctcgtg
     1621 ccggtctcgg tgcgcaccgc cgaggacgag ggcgagctgg gcaaccgcat ctcgaccgtg
     1681 ttcgtggacc tgcccatgag cggcgagccg ctggagcgcc tggagcgcgt cagcgccgcc
     1741 atggacgacg tcaagggctc ggcccaggtg cgcgcgggcg cgctgatcgt cggtgccacc
     1801 gggctggcgc ccccggtcgt gtcctcgctg gccgtccggg ccatgagcgg cccgcgcatg
     1861 ttcaacctgg tggtctccaa cgtgcccggg ccgcagcaga ccctgtacct ggacgggacg
     1921 ccgctgcgcg aggtctaccc cgccgtgccg ctgaacccgc gcaaccaggc gctgtcgatc
     1981 ggcatcgtca gctacgacgg cggcgtgggc ttcggcctcc tgggcgaccg cgacgcgctg
     2041 tcagacatcg gggacgcggc cgcgggcctc gaggcgacgg tggccgagct ggtgcacgcc
     2101 gcgaccggct gatggcgacc gcatccgacg tgctggcctt ccgggtggag cgccaccacc
     2161 tggcccggcg ggtcgcggac gcccgggtgg ccgccggcgt ggggctgcag gacacgccgc
     2221 cggggaccgc ggcgctggcg ctggccgcgc gggccgaggt gggccccgag gcgctggacg
     2281 acctggtgat cgtgcccagc gtgcgcggcg ccccgctggc cgtggcgccg gaggacctgc
     2341 cgctgttcac cgcgggcctg gcgccgccgg atgaggacgc ggccaaggcg gtgatcggca
     2401 acgcgtggaa ggaactggac ggcgtgaccg ccatggacgc gctcgaccgc gtcagcgacg
     2461 ccgtccgcga cagcctcgcc tccggccccc tggcccgcga cgacttccac caggcgctgc
     2521 gcgaccggct cccggacgag ctgctgtggt ggtgcaaggg ctgccagagc caccacgtgc
     2581 acccgtcgct gtggcgcgcc accggcatcc gcggcgtgct ggcgatcgtc ggccgcgagg
     2641 ggcgcagcgc ggtgttcggc gcgccaccgc ccgcgccgga ggcggtcgcc gatcccggcg
     2701 cggcgctggc ccgccggttc ctgggcgcct acgggcccgc gcggccgaag ctgctggccg
     2761 aatgggccgg gatcaccacc gcgcacgccc gcgcgctctg ggagcgggcc ggcgagctgg
     2821 cggccgtcga ggtcgatggt cgtgaggcgt gggtgctggc cgaggacgcg acggcgctgg
     2881 ccgagccggc ccccgcggag ggcgtgcggc tcctgcccaa cctcgatccc ctggccggct
     2941 cccgcgaccg cgaggtcctg gtcccggacg ccgaactgcg caaacgggtc tggggccgtc
     3001 tgggcgggcc gggcctggtc ctggtcgacg gcgccgtcgc cggcctgtgg cgcccggcga
     3061 agaagggcag gaagctggtg gtcaccgtgg aggccacggg ccggatcagc cgggccgtga
     3121 aggacgccct ggccggggag gccgagcgca tcgcgccctt ccgcggtgcc gagtcggccg
     3181 agcttgcgta aagtgtccgg tccccacgcg gatgtggcgg aactggtaga cgcgcaggct
     3241 tcaggtgcct gtgtcctttg ggacgtggag gttcgactcc tctcatccgc atggcccggc
     3301 cctcaccgcc ggccgatcag cttggccagc tccgcggcgg tgctctcggg gtggtcgggc
     3361 agccggtcca gcgcctcggc cacctggtag acgtcctcga tcccgacccc cttggcctcc
     3421 aggcagaagc ggccgatcca gcggatggcc gcccgctcgt acagctcggg ctcgtcgcga
     3481 taggccacgc agatgtccag ggcgtcctcc aggcggacgg ccggcagctg gcgcgcggtg
     3541 cgccggatcc ggtccaggtt gccgctgttg agcgcggagc ggaaggcggc gtaggcggga
     3601 ccgggcgcgg acacgaacac acgttcgcat agatcgggga gctcgtgggc gcccgcttgc
     3661 gtcgcccgga cccggctagc gttgccggca tggacgcagc ggtactcacg gagctcggca
     3721 cacccgcatt cggcacctac gacgagccca ccgcgggcga cg
//
LOCUS       DASUPW010000557         2982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1929532, whole genome shotgun sequence.
ACCESSION   DASUPW010000557 DASUPW010000000
VERSION     DASUPW010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1929532"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /gene="pdxT"
                     /locus_tag="VFY44_12545"
     CDS             <1..351
                     /gene="pdxT"
                     /locus_tag="VFY44_12545"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF013350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEX5911314.1"
                     /translation="PTLGTCAGLIMLDRDHLDLMDIRAERNAFGRQVKSFESDIDLEG
                     LGEGFHGVFIRAPWVAEHGDGVEVLAQVDGHPVAVSSGNVLAVAFHPEIDGDTRLHEW
                     LLARVQSNSERGPQ"
     gene            348..1451
                     /locus_tag="VFY44_12550"
     CDS             348..1451
                     /locus_tag="VFY44_12550"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX5911315.1"
                     /translation="MRDQRADALAQILVRYSTKVQKGDVCVIQSTTTAEPLVQAVYEE
                     VLRAGGLPIFNLTTEEAAAALYELASDDQLDWVPPTSRWFAENADVRIVLMADANTRA
                     LSQADPKKQARSQRARKPLMDASMKRSAAGEHRWALTLFPTHAYAAEAGMSLGQYEDF
                     YYRACLCDDPDPVTAWERQSDEVKRLAAWIEGKEEVHISAPGTDITLGIAGRSWVPCA
                     GSHNMPDGEFFTGPVEDSVQGEVSFSFPATYAGREVAGVKLRFEAGKVVEATAERGEE
                     FLNEMLDTDEGARRLGELGIGTNYGIATGTKEILLDEKIGGTVHMAVGASYPETGGVN
                     DSAVHWDMVCDLRQGGVITVDGQELSRDGKFVV"
     gene            1473..2228
                     /locus_tag="VFY44_12555"
     CDS             1473..2228
                     /locus_tag="VFY44_12555"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX5911316.1"
                     /translation="MSGHSKWSSIKHKKGAADAKRGALFTKLTRAITVAAREGGGDVE
                     GNPSLALAVQKAKDNSMPKDNIERAINKGTGADQDADAFERIVYEGYGPGGAALLVDA
                     LTDNRNRTGSEVRHIFSKFNGNLGEPGSVNYLFERKGELIVDASLYSEDDLMAAIDAG
                     AEDVVEDGDVFEVVTDHTDLTSVRKALEDAGVQIQSSEIRMRPTLRTPVEEGDAGTLM
                     KLIDALEENDDVQAVHGNFDVDDAVLERVASAG"
     gene            2266..2760
                     /gene="ruvC"
                     /locus_tag="VFY44_12560"
     CDS             2266..2760
                     /gene="ruvC"
                     /locus_tag="VFY44_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX5911317.1"
                     /translation="MVVVLGIDPGIANTGYGVVLTRGSMLAALDGGVIATAARTPPEQ
                     RLARIHADVCALIEEHRPDAMAIEELYFGANASSAFAVGQARGVVLLAAGAAGIPCFS
                     YTPQQIKQAVCGSGRAAKDQVQRMVGTLLSLPAPPEPDHAADALAVGICHANTAPFRA
                     AVPA"
     gene            2757..>2982
                     /locus_tag="VFY44_12565"
     CDS             2757..>2982
                     /locus_tag="VFY44_12565"
                     /inference="COORDINATES: protein motif:HMM:NF013495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX5911318.1"
                     /translation="MIAHVSGEVMLRRDGEVVLDTAGGIGYRLSVSSETLAKVPRKGG
                     SVTLHSHLVVRDDAMQLYGFASEQERDLFLM"
BASE COUNT          480 a         1051 c         1058 g          393 t
ORIGIN      
        1 cccacgctcg gcacctgcgc cggcctgatc atgctcgacc gcgaccacct cgacctcatg
       61 gacatccgcg ccgagcgcaa cgccttcggg cgccaggtca agagcttcga gtccgacatc
      121 gacctcgagg gcctgggcga aggcttccac ggcgtgttca tccgcgctcc gtgggtggcc
      181 gagcacgggg acggggtgga ggtgctcgcg caggtcgacg ggcacccggt ggccgtgagc
      241 tccggcaacg tgctggccgt ggccttccac cccgagatcg acggtgacac ccgcctgcac
      301 gagtggttgc tcgcgcgggt ccaatccaac tccgaaaggg ggccgcagtg agagaccagc
      361 gcgccgacgc cctggcccag atcctcgtcc gctactccac caaggtgcag aagggcgacg
      421 tgtgcgtgat ccagtcgacc accaccgcgg agccgctggt gcaggcggtg tacgaggagg
      481 tcctgcgcgc gggcgggctg cccatcttca acctgaccac cgaggaggcc gcggccgcgc
      541 tgtacgagct ggccagcgac gaccagctgg actgggtgcc gcccacctcg aggtggttcg
      601 ccgagaacgc cgacgtgcgc attgtgctga tggccgacgc caacacgcgc gcgctgtccc
      661 aggccgaccc caagaagcag gcccgctcgc agcgggcccg caagccgctg atggacgcgt
      721 ccatgaagcg ctcggccgcc ggtgagcacc gctgggcgct gacgctgttc cccacgcacg
      781 cctacgccgc cgaggcgggc atgtcgctgg gccagtacga ggacttctac taccgcgcct
      841 gcctgtgcga cgaccccgac cccgtgaccg cgtgggagcg ccagtccgac gaggtcaagc
      901 ggctggcggc gtggatcgag ggcaaggagg aggtgcacat cagcgccccc gggaccgaca
      961 tcacgctggg catcgcgggc cgctcgtggg tgccgtgcgc cggcagccac aacatgcccg
     1021 acggcgagtt cttcaccggc ccggtcgagg attcggtcca gggcgaggtg tcgttcagct
     1081 tcccggccac ctacgccggc cgcgaggtcg cgggcgtgaa gctgcgcttc gaggcgggca
     1141 aggtcgtgga ggccaccgcc gagcgcggcg aggagttcct gaacgagatg ctggacaccg
     1201 acgagggcgc gcgccggctg ggcgagctcg gcatcggcac caactacggc atcgccaccg
     1261 ggaccaagga gatcctgctg gacgagaaga tcggcggcac ggtgcacatg gcggtgggcg
     1321 cctcctaccc cgagaccggc ggcgtcaacg actccgcggt gcactgggac atggtctgcg
     1381 acctgcgcca gggcggcgtg atcacggtcg acggccagga gctgtcgcgt gacggcaagt
     1441 tcgtggtctg aaccgcttta gactcgactg ccatgtccgg tcactccaaa tggtcctcga
     1501 tcaagcacaa gaagggcgcc gccgacgcca agcgcggcgc gctgttcacc aagctcacgc
     1561 gcgccatcac cgtcgcggcg cgtgagggcg ggggagacgt cgagggcaac ccgtccctgg
     1621 ccctggccgt ccagaaggcc aaggacaact cgatgcccaa ggacaacatc gagcgggcga
     1681 tcaacaaggg caccggcgcc gaccaggacg ccgacgcatt cgagcggatc gtctacgagg
     1741 gctacgggcc cggcggcgcc gcgctgctgg tggacgcgct gaccgacaac cgcaaccgca
     1801 ccggctccga ggtgcgccac atcttctcca agttcaacgg caacctgggc gagcccgggt
     1861 cggtgaacta cctgttcgag cgcaagggcg agctgatcgt ggacgcctcg ctgtactccg
     1921 aggacgacct gatggccgcc atcgacgccg gcgccgagga cgtggtcgag gacggcgacg
     1981 tgttcgaggt cgtgaccgac cacaccgacc tgacctcggt gcgcaaggcg ctcgaggacg
     2041 ccggggtgca gatccagtcc tccgagatcc ggatgcgccc cacgctgcgg accccggtcg
     2101 aggagggcga cgcgggcacg ctgatgaagc tgatcgacgc gctcgaggag aacgacgacg
     2161 tgcaggccgt gcacggcaac ttcgacgtgg acgacgccgt gctcgagagg gtcgcctccg
     2221 cgggctaggc gggaggcatc cgatcgcctg cccagaatca gggcagtggt ggtcgtcctc
     2281 ggcatcgatc ccggtatcgc caacaccggc tacggcgtgg tgctcacgcg cgggagcatg
     2341 ctcgcggcgc tggacggcgg ggtcatcgcg accgccgcca ggaccccgcc cgagcagcgg
     2401 ctggcccgga tccacgccga cgtctgcgcg ctgatcgagg agcaccgccc ggacgccatg
     2461 gccatcgagg agctctactt cggcgccaac gccagcagcg cgttcgcggt cggccaggcc
     2521 cggggcgtcg tgctgctggc cgccggcgcg gccgggatcc cgtgcttctc ctacacgccg
     2581 cagcagatca agcaggcggt ctgcggctcg ggtcgcgccg ccaaggacca ggtgcagcgt
     2641 atggtcggga ccctgctgag cctgcccgcg ccacccgagc ccgaccacgc cgccgacgcc
     2701 ctggcggtgg ggatctgcca cgccaacacg gcgcccttcc gcgccgcggt gccggcgtga
     2761 tcgcccacgt cagcggcgag gtgatgctgc gccgcgacgg cgaggtcgtc ctggacaccg
     2821 ccgggggcat cggctaccgg ctgtccgtgt cctccgagac gctggccaag gtgccgcgca
     2881 agggcggctc ggtcaccctt cactcccacc tggtggtccg cgacgacgcg atgcagctct
     2941 acggcttcgc cagcgagcag gagcgcgacc tgttcctgat gc
//
LOCUS       DASUPW010000558         2822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1929654, whole genome shotgun sequence.
ACCESSION   DASUPW010000558 DASUPW010000000
VERSION     DASUPW010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1929654"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..174)
                     /locus_tag="VFY44_12570"
     tRNA            complement(101..174)
                     /locus_tag="VFY44_12570"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(138..140),aa:Ile,seq:cat)
     gene            complement(195..848)
                     /locus_tag="VFY44_12575"
     CDS             complement(195..848)
                     /locus_tag="VFY44_12575"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HEX5911319.1"
                     /translation="METPTVKTFVLEPPMWMPHLPGQHYDVRLTAPDGYTAQRSYSIA
                     SSPLDEGRVELTIDCLADGEVSPYFHDVVQEGDQVEVRGPFASYFVWRGEAPVLLIGG
                     GSGVVPLMAMLRHRRRAMPELPMRLVYSVRNAEDVIYASELEADADLTFSRSAPEGWS
                     GHTGRIDPGLIGDPGLGDAGLAFVCGSNPFVEAASMMLLDLGVAPGQIRTERFGPTS"
     gene            complement(908..1519)
                     /locus_tag="VFY44_12580"
     CDS             complement(908..1519)
                     /locus_tag="VFY44_12580"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEX5911320.1"
                     /translation="MSGFVSRGFGGRRRVPDELAGRLPPGQYVEPGFPVLSAGPTERI
                     APQEWRMRIDGMVAEPLDLSWDELRALPSEQVPCDIHCVTKWSKFDTSFTGVPVNTLL
                     DRVQPQGRFAMAHSYGGYTTNLALEDIGAGKAWVVWEHEGEPLSADHGGPVRMIVPHL
                     YFWKSAKWLAGLRIMDHDEPGFWEENGYHNRGDPWKEERYWSD"
     gene            1538..1915
                     /locus_tag="VFY44_12585"
     CDS             1538..1915
                     /locus_tag="VFY44_12585"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX5911321.1"
                     /translation="MTENRKPTVEVPEGAPSYQLEVEDITVGDGDEAAAGHKVEVHYV
                     GVSWQSGEQFDASWDRGDTFKFGLGKGQVIQGWDEGVVGMKVGGRRKITIPPMLAYGK
                     RGAGGVIGPDETLIFVVDLIGLS"
     gene            complement(1919..>2822)
                     /locus_tag="VFY44_12590"
     CDS             complement(1919..>2822)
                     /locus_tag="VFY44_12590"
                     /inference="COORDINATES: protein motif:HMM:NF009472.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding dehydrogenase"
                     /protein_id="HEX5911322.1"
                     /translation="VPAHVDGRMLEIAKSAGANEINGDRMWHYVEGIHNWDPIWPMHA
                     IRIIPGPSSLWFDATGKRLPGPLYPGYDTLGTLAHIQTTGHDYTWFVLTEKIIEKEFA
                     LSGSEQNPDTTSKSIKAVLKDRLGSGPPAPVQAFKDHGQDFIVERNLPDLVRRMNELT
                     GDDLIDLGDLEAQIVARDREIDNPYAKDGQVTAIHGARAYLADKITRVASPHKILDPD
                     AGPLIAVRLSILTRKSLGGLQTDLDARVLTSDGSPLQGLYAAGEVAGFGGGGMHGYRA
                     LEGTFLGGCLFSGRTAGRSLARAL"
BASE COUNT          407 a          964 c         1032 g          419 t
ORIGIN      
        1 gaaggagcgg aaacgtgcac catgtgcggg gcgaccgtgc cgccttccgc cagcgccgcc
       61 cagcaggggc cgaagtcgtg ccgcctcgtc tgactctcga tgggccgggc aggacttgaa
      121 cctgcgcacg acggattatg agtccgctgc tctaaccagc tgagctaccg gcccgcgcgc
      181 ccgatcctac gggctcagct cgtcggcccg aaccgctccg tgcggatctg gccgggcgcg
      241 acgcccaggt ccagcagcat catcgaggcc gcctccacga acgggttcga gccacagacg
      301 aaggccaggc cggcgtcgcc caggccgggg tcgccgatca gcccggggtc gatgcggccc
      361 gtgtggccgc tccagccctc gggggcggag cgcgagaagg tgaggtcggc gtcggcctcc
      421 agttcggacg cgtagatcac gtcctcggcg ttacgcacgg agtagacgag ccgcatcggc
      481 agctcgggca tcgcgcggcg gcggtggcgc agcatcgcca tcagcgggac cacaccggac
      541 ccgccgccga tcagcagcac cggggcctcg ccgcgccaga cgaagtagga ggcgaagggt
      601 ccgcggacct cgacctgatc gccctcctgg acgacgtcgt ggaagtaggg cgagacctcg
      661 ccgtcggcca ggcagtcgat ggtcagctcg accctcccct cgtccagcgg ggacgacgcg
      721 atcgagtagg agcgctgggc ggtgtagccg tcgggagcgg tcaggcggac gtcgtagtgc
      781 tgccccggca ggtggggcat ccacatcggc ggctcgagca cgaacgtctt gacggtcggc
      841 gtctccacgc ggatgccggc gacggtggct atctgccagg cgccgggcgc ccgcgggacg
      901 ggttcggtca gtcgctccag taccgctctt ccttccaggg gtcgccgcgg ttgtggtagc
      961 cgttctcctc ccagaagccg ggctcgtcgt ggtccatgat ccgcaggccc gcgagccact
     1021 tggcgctctt ccagaagtac aggtgaggca cgatcatgcg gacgggcccg ccgtggtcgg
     1081 ccgacagcgg ctcaccctcg tgctcccaca ccacccacgc cttgccggcg ccgatgtcct
     1141 cgagcgcgag gttggtggtg tagccgccgt aggagtgcgc catggcgaac cggccctgcg
     1201 gctggacgcg gtcgaggagc gtgttcacgg ggaccccggt gaagctggtg tcgaacttcg
     1261 accacttggt cacgcagtgg atgtcgcacg ggacctgctc ggagggcagc gcacggagct
     1321 cgtcccacga gaggtccagc ggctcggcca ccatgccgtc gatgcgcatc cgccactcct
     1381 gcggcgcgat ccgctcggtc ggcccggcgc tgaggacggg gaacccgggc tccacgtact
     1441 ggccgggcgg cagcctgccg gccagctcgt cggggaccct gcgcctgccg ccgaagcccc
     1501 gggagacgaa tccgctcatt cgcgtagcct gcctgccgtg accgagaacc gcaagcccac
     1561 cgtcgaagtc cccgaaggcg cgccgtcgta ccagctggag gtggaggaca tcaccgtcgg
     1621 cgacggcgac gaggccgccg ccggccacaa ggtcgaggtc cactacgtgg gcgtctcctg
     1681 gcagtcgggc gagcagttcg acgcctcctg ggaccgcggc gacacgttca agttcggcct
     1741 cggcaagggc caggtcatcc agggctggga cgagggcgtc gtgggcatga aggtcggcgg
     1801 ccggcgcaag atcacgatcc cgccgatgct ggcctacggc aagcgcggcg ccggcggcgt
     1861 gatcgggccc gacgagacgc tgatcttcgt cgtggacctg atcggcctga gctagccgct
     1921 agagcgctcg cgccagcgac cggccggcgg tccggcccga gaacaggcaa ccgcccagga
     1981 acgtgccctc cagcgcgcgg tagccgtgca tgcccccgcc gccgaagccc gcgacctcac
     2041 cggcggcgta gagcccctgc agcggcgagc cgtcggaggt gagcacgcgc gcgtccaggt
     2101 cggtctgcag gccgccgagc gacttgcgcg tgaggatcga gagccggacg gcgatcagcg
     2161 gcccggcgtc ggggtccagg atcttgtgcg gtgacgcgac gcgcgtgatc ttgtcggcca
     2221 ggtaggcgcg ggcgccgtgg atggcggtga cctggccgtc cttggcgtac gggttgtcga
     2281 tctcacggtc gcgggccacg atctgggcct ccaggtcgcc caggtcgatc aggtcgtcgc
     2341 ccgtgagctc gttcatgcgc cgcaccaggt cgggcaggtt gcgctcgacg atgaagtcct
     2401 ggccgtggtc cttgaacgcc tgcaccgggg cgggcgggcc gctgcccaga cggtccttga
     2461 gcactgcctt gatgctcttc gacgtggtgt cggggttctg ctcggagccc gacagggcga
     2521 actccttctc gatgatcttc tcggtgagca cgaaccacgt gtagtcgtgg ccggtcgtct
     2581 ggatgtgggc cagcgtgccc agcgtgtcgt agccggggta cagcgggccc ggcaggcgct
     2641 tgccggtggc gtcgaaccag agcgaggacg ggcccgggat gatccggatg gcgtgcatgg
     2701 gccagatcgg gtcccagttg tggatgccct cgacgtagtg ccacatgcgg tcgccgttga
     2761 tctcgttcgc gcccgcgctc ttggcgatct cgagcatccg cccgtccacg tgggcgggca
     2821 cg
//
LOCUS       DASUPW010000559         4280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1237864, whole genome shotgun sequence.
ACCESSION   DASUPW010000559 DASUPW010000000
VERSION     DASUPW010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237864"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1683)
                     /gene="carB"
                     /locus_tag="VFY44_12595"
     CDS             complement(<1..1683)
                     /gene="carB"
                     /locus_tag="VFY44_12595"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX5911323.1"
                     /translation="MPRRDDISKILVIGSGPIIIGQAAEFDYSGAQACKVLLEEGYEV
                     VLINSNPATIMTDPEFATATYIEPLLPDSVTRVIDKERPDALLPTLGGGTALNLARQL
                     TDDGTLERYGVELIGADYDAIQRAEDRELFNATMEAAGLRVARSSIAHTLAEAEAVRE
                     DIGLPAIVRPGFTMGGQGGGIARTEAEYHERVAEGLAASPINQVLIDESVIGWGEFEL
                     ELMRDKADNVVVICSIENVDPMGVHTGDSVTVAPQQTLSDIQYQALRDQAITVIRAVG
                     VETGGSNVQFAVNPETDEIIVIEMNPRVSRSSALASKATGFPIAKIAARLAVGYTLEE
                     IDNDITRVTPASFEPTIDYVVVKWPRFAFEKFPGSDGKLSTYMQSVGEAMAIGRTFKQ
                     AWMKAWRSRELDVRATPPADDEELLTALEIPSWDRYELVFEAFRQGITQEQVHAATQI
                     DPWYLRELHQLATDPDHEMEGRVRTYKAVDTCAAEFEAATPYFYSGWERPGPHGPRHE
                     VERGDNASVVILGSGPNRIGQGIEFDYCCVHATQALRELGLETIMINNNPETV"
     gene            complement(1673..2818)
                     /gene="carA"
                     /locus_tag="VFY44_12600"
     CDS             complement(1673..2818)
                     /gene="carA"
                     /locus_tag="VFY44_12600"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX5911324.1"
                     /translation="MSAAPGYLLLEDGERFDGDLVGALEHGRGEVVFNTAMSGYQESV
                     TDPSYRGQIIVFTYPMVGNYGVQAAHMESSDAHARAVIMREGVDRDDAPGATAGWLTW
                     LAERGIPALTGVDTRALVRHIRDKGAMRGGVFDAQTDRTQAMEWLMAEPPMQGQDLAR
                     EVTPGEPILLDPLDGSEGGPRVVAIDTGIKWSIVRNLRERGAYVELHPCTITADQVLA
                     REPDAVFLANGPGDPAALGYVVDTVRELVGTKPVWGICLGHQLLCRAVGLDTFKLPFG
                     HRGANHPVKDLETGKVEITSQNHGFAVLGPGGEQTIDGGESMRWETDFGTAELSQLNL
                     YDRTVEGLVLRDVPASTVQYHPEAGPGPHDARHLFDRFLQRVAAGAA"
     gene            complement(2815..3342)
                     /locus_tag="VFY44_12605"
     CDS             complement(2815..3342)
                     /locus_tag="VFY44_12605"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX5911325.1"
                     /translation="MLERDRTTVLVVDVQEAFRKGVEHFDDVAKGSAVLLQAAQIMGL
                     PVVVTEQYPKGLGATVDEVARHLGDVVPLPKTVFSGASADGFDLGGRDQVLMCGIEAH
                     VCVHQTAAELLAGGVEVHVAVDAVGSRSALDREVGLRRMTDAGAVPTTVETALFEMFG
                     KAGGDEFKQVQGLVK"
     gene            complement(3342..>4280)
                     /locus_tag="VFY44_12610"
     CDS             complement(3342..>4280)
                     /locus_tag="VFY44_12610"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX5911326.1"
                     /translation="GFTAAVTVGQKGEQLTEAAELAAAGAAGLTDDGLPIRSAGLMRQ
                     ALQYQRLAGLTIALHEEDPTLSAGGVMHEGEVSALLGVAGVPSVSESVMVARDCDLAA
                     YEDGRIHVQHVSARTTVEIIERAKAAGVKVTAEATPHHLLLSDEAVRTLDTNKKMNPP
                     LRSEDDRQAIIEGVRSGALDTIATDHAPHARDEKEQPFEDAPLGVTGLETAFASVYTD
                     LVVPGVLDLGVVVERMTAGGEPFGLPVPTLAEGRAADLCLVDLDSQWQVGEAGYESRS
                     ANCAFHGRTLTARVRMTIAGGAVAFRERSFAISGVS"
BASE COUNT          552 a         1516 c         1545 g          667 t
ORIGIN      
        1 caccgtctcg gggttgttgt tgatcatgat cgtctcgagc ccgagctcgc ggagcgcctg
       61 cgtcgcgtgg acgcagcagt agtcgaactc gatgccctgc ccgatccggt tggggcccga
      121 gcccaggatg accaccgagg cgttgtcgcc gcgctcgacc tcgtggcgcg ggccgtgggg
      181 ccccggccgc tcccagccgg agtagaagta gggcgtggcg gcctcgaact cggccgcgca
      241 ggtgtcgacc gccttgtagg tgcggacgcg tccctccatc tcgtggtccg ggtcggtcgc
      301 gagctggtgg agctcgcgca ggtaccacgg gtcgatctgc gtggcggcgt ggacctgctc
      361 ctgcgtgatg ccctgccgga acgcctcgaa gaccagctcg tagcggtccc aggacgggat
      421 ctcgagcgcg gtcagcagct cctcgtcgtc ggccggcggg gtggcgcgca cgtccagctc
      481 gcgcgagcgc caggccttca tccacgcctg cttgaaggtg cggccgatgg ccatcgcctc
      541 ccccaccgac tgcatgtagg tggacagctt gccgtcggag ccggggaact tctcgaaggc
      601 gaaccggggc cacttgacaa ccacgtagtc gatggtgggc tcgaacgacg cgggcgtgac
      661 gcgggtgatg tcgttgtcga tctcctcgag cgtgtagccc accgccagcc gcgcggcgat
      721 cttggcgatc gggaacccgg tggccttcga ggccagcgcg gacgagcgcg acacgcgcgg
      781 gttcatctcg atgacgatga tctcgtcggt ctcggggttc acggcgaact gcacgttgga
      841 gccgccggtc tcgaccccga cggcgcggat cacggtgatg gcctggtcgc gcagcgcctg
      901 gtactggatg tcggagagcg tctgctgcgg ggccacggtg acgctgtcgc cggtgtggac
      961 ccccatcggg tccacgttct cgatcgagca gatgaccacg acgttgtcgg ccttgtcgcg
     1021 catcagctcg agctcgaact cgccccagcc gatgacggac tcgtcgatca gcacctggtt
     1081 gatcgggctg gcggccaggc cctcggccac gcgctcgtgg tactcggcct cggtccgggc
     1141 gatgcctccc ccctggccgc ccatcgtgaa gcccggccgc acgatggccg gcaggccgat
     1201 gtcctcgcgc acggcctcgg cctcggccag cgtgtgggcg atcgaggagc gggccacgcg
     1261 caggccggcg gcctccatcg tcgcgttgaa cagctcgcgg tcctcggcgc gctggatggc
     1321 gtcgtagtcg gcgccgatca gctcgacgcc gtagcgctcg agcgtgccgt cgtccgtgag
     1381 ctggcgggcg aggttcaggg cggtgccgcc acccagggtg ggcagcagcg cgtcggggcg
     1441 ctccttgtcg atcacccgcg tcacagagtc gggcagcagg ggctcgatgt aggtggccgt
     1501 ggcgaactcc gggtcggtca tgatcgtcgc cgggttggag ttgatcagga cgacctcgta
     1561 gccctcctcc agcaggacct tgcaggcctg cgcgccggag tagtcgaact cggcggcctg
     1621 gccgatgatg atcggccccg atccgatgac caggatcttg ctgatgtcgt ccctacgcgg
     1681 caccggcggc caccctctgc aggaagcggt cgaacaggtg cctggcgtcg tgcgggcccg
     1741 ggccggcctc ggggtggtac tgcacggtgg acgcgggcac gtcgcgcagg accaggccct
     1801 cgacggtgcg gtcgtagagg ttgagctggc tcagctcggc ggtgccgaag tcggtctccc
     1861 agcgcatcga ctcgccgccg tcgatggtct gctcgccgcc cgggcccagc accgcgaagc
     1921 cgtggttctg gctggtgatc tccaccttgc cggtctccag gtccttgacc gggtggttgg
     1981 cgccgcggtg gccgaagggc agcttgaacg tgtccaggcc cacggcccgg cacagcagct
     2041 ggtggcccag gcagatgccc catacgggct tcgtgcccac cagctcgcgg acggtgtcga
     2101 ccacgtagcc cagggcggcc gggtcaccgg ggccgttggc caggaacacc gcgtcgggct
     2161 cgcgcgccag tacctggtcg gcggtgatgg tgcaggggtg cagctccacg taggcgccgc
     2221 gctcgcgcag gttgcgcacg atcgaccact tgatgccggt gtcgatggcc accacccgcg
     2281 ggccgccctc gctgccgtcg agcgggtcca gcaggatcgg ctcgcccggc gtgacctcgc
     2341 gggccaggtc ctggccctgc atcggcggct cggccatgag ccactccatg gcctgcgtgc
     2401 ggtcggtctg ggcgtcgaag acgccgccgc gcatggcgcc cttgtcgcgg atgtggcgca
     2461 ccagcgcgcg ggtgtccacg ccggtcagcg cggggatgcc gcgctcggcc agccaggtca
     2521 gccagccggc ggtggcgccg ggggcgtcgt cgcggtccac gccctcgcgc atgatcacgg
     2581 cgcgggcatg ggcgtcggag gactccatgt gcgccgcctg gacgccgtag ttgcccacca
     2641 tcgggtaggt gaagacgatg atctggccgc ggtagctggg gtcggtgacc gactcctggt
     2701 agcccgacat ggcggtgttg aacaccacct ctcctctgcc gtgctcgagc gcccccacga
     2761 ggtcgccgtc gaagcgctcg ccgtcctcca gcagcaggta tccgggtgcg gcgctcactt
     2821 gaccagcccc tggacctgct tgaactcgtc gccgccggcc ttgccgaaca tctcgaacag
     2881 ggccgtctcg acggtggtgg gcaccgcgcc cgcgtcggtc atccgccgca ggccgacctc
     2941 acggtccagc gccgagcgcg agccgaccgc gtccacggcc acgtgcacct cgaccccgcc
     3001 ggccagcagc tcggccgcgg tctggtgcac gcacacgtgc gcctcgatgc cgcacatcag
     3061 gacctggtcg cgcccgccga ggtcgaagcc gtcggccgag gcgccggaga agacggtctt
     3121 gggcagcggc acgacgtcgc ccaggtgacg ggcgacctcg tccacggtgg ccccgagacc
     3181 cttcgggtac tgctcggtga ccacgacggg caggcccatg atctgcgccg cctgcagcag
     3241 cacggctgag cccttggcga cgtcgtcgaa gtgctcgacg cccttgcgga aggcctcctg
     3301 gacgtcgacc acgagcaccg tggtgcggtc tcgctccagc atcagctgac ccccgagatg
     3361 gcgaagctcc gctcgcggaa ggccaccgcg ccgccggcga tggtcatccg cacccgcgcg
     3421 gtcagcgtcc ggccgtggaa ggcgcagttg gccgagcggc tctcgtagcc ggcctccccc
     3481 acctgccact gcgagtccag gtcgaccagg cacaggtcgg cggcgcgccc ttcggccagc
     3541 gtgggcaccg gcagcccgaa cggctcgccg cccgcggtca tgcgctcgac caccacgccc
     3601 aggtccagga cgcccggcac caccaggtcg gtgtagaccg acgcgaacgc ggtctcgagc
     3661 ccggtgacgc ccagcggcgc gtcctcgaac ggctgctcct tctcgtcgcg tgcgtgcggg
     3721 gcgtggtcgg tggcgatggt gtccagcgcc ccggagcgaa cgccttcgat gatggcctgg
     3781 cggtcgtcct cgctgcgcag cggagggttc atcttcttgt tcgtgtccag cgtgcgcacc
     3841 gcctcgtcgc tgagcagcag gtggtgcggc gtggcctcgg cggtgacctt gaccccggcg
     3901 gccttggccc gctcgatgat ctccacggtg gtgcgggcgg acacgtgctg cacgtggatc
     3961 cgcccgtcct cgtaggcggc caggtcgcag tcgcgggcca ccatgaccga ctcgctgacc
     4021 gacgggacgc cggcgacgcc cagcagggcg gacacctcgc cttcgtgcat cacgccgccg
     4081 gcggacaggg tcgggtcctc ctcgtgcagc gcgatggtca gcccggccag gcgctggtac
     4141 tgcagggctt ggcgcatcag gcccgccgag cggatgggca ggccgtcgtc ggtcagccct
     4201 gcggcgccgg cggcggccag ctcggcggcc tcggtcagct gctcgccttt ctggccgacc
     4261 gtcacggccg ctgtgaagcc
//
LOCUS       DASUPW010000560         3682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_959365, whole genome shotgun sequence.
ACCESSION   DASUPW010000560 DASUPW010000000
VERSION     DASUPW010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959365"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /locus_tag="VFY44_12615"
     CDS             complement(<1..1361)
                     /locus_tag="VFY44_12615"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HEX5911327.1"
                     /translation="MTLLAIGDGTNTLALVVFAIVLAITLGITYWASKRTSTATEFWA
                     AGRGISGLQNGFAMAGDYLSASTFLGFAGLIFLFGVDGWVGLAAAGASFLPVVLLLAE
                     RMRNSGKFTMADVLAFRLNARPARSAAAMGTLVVSFIYLVAQMVGAGVLLQALAGIDF
                     VFSVLITGTFMVVYVVAGGMLATTWVQIIKAGLLMAAGLVLTILVLNEVSFSPTELFD
                     RAAGEYGKGADAYLSPGQQNPSAWSSVSFGLALLLGTAGLPHILMRFFTVPDSAAARK
                     SIAWAVALIGAFFLMTMIVGVGARAILGAGAEEASAGGNLATPLLAEELGGGAGTTGG
                     DLFLAFISAVAFATILAVVARLVIAASGAVAHDLWTGVKRNGVEDEQEEVKVARITAV
                     GLGVVAILVSIAAGEEFNVSLLIGLAFAVAASANLPPLLCTLFWPRFNTMGAVAGVLG
                     GLVV"
     gene            complement(1358..1684)
                     /locus_tag="VFY44_12620"
     CDS             complement(1358..1684)
                     /locus_tag="VFY44_12620"
                     /inference="COORDINATES: protein motif:HMM:NF016244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HEX5911328.1"
                     /translation="MSETFDWTAIEGSAEFRELTKGRRRFSAVAGSIGIGAGLLYIFL
                     AGFARDFMGTEVIGSISLAFVGGVALILLTWLITFLYMRRSARVWSPMEERIRQAAAT
                     SGEARS"
     gene            1776..2978
                     /locus_tag="VFY44_12625"
     CDS             1776..2978
                     /locus_tag="VFY44_12625"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5911329.1"
                     /translation="MPTDPMPYEHLGEALATDYFFVREQFDDEQWAHFIETRRFVDTE
                     VLPAINDYWVAEEMPWDLIRRLPELGLVGEDIQGYGCPGMSPLTRGLVNMELHRGDGS
                     LGTFLGVQSGLAMKSIAMLGSEEQKETWLPAMAELDKVGAFALTEPEHGSDSVALETS
                     ARREGDEWVLDGAKRWIGNASVADVTVVWARDAEDEQVKGFLVEGDAPGFTATTMTGK
                     GASRAIWQADITLEGVRVPEEMRLPEAASFKQTGAVLMSTRTTCAWSALGHAVAAYDA
                     ALAYAGQREQFGKPLVGFQLIQDRLVRMLSEVTAMQLYCMQLARLEEADRLTPTIAGL
                     AKLHNTRKARGVIAEARDLMGGNGLLLENHVIRHMGDIEVIHTYEGTETMQSLIVGRD
                     ITGVAAFT"
     gene            3082..>3682
                     /locus_tag="VFY44_12630"
     CDS             3082..>3682
                     /locus_tag="VFY44_12630"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX5911330.1"
                     /translation="MAEALVFDAIRTPRGKGKSNGSLHTTKPVDLVVGLMHETLGRNV
                     QLDPSKVDDVVLGVVSPVGDQGADIAKTAAIKAGLPDTVAGVQLNRFCASGLEAVNIA
                     AQKVASGWEDLVFAGGVESMSRVPMGSDGGAWAMDPETNYDTSFIPQGVGADLIATVE
                     EFSRDDVDAYAARSQERAAAAQAEGRFDNSVIPVKDINDQ"
BASE COUNT          614 a         1302 c         1303 g          463 t
ORIGIN      
        1 acgaccaggc cgcccagcac gcccgcgacc gcgcccatgg tgttgaaccg gggccagaac
       61 agcgtgcaca gcagcggcgg caggttggcg ctggcggcca cggcgaaggc caggccgatg
      121 agcagcgaga cgttgaactc ctcgcccgcg gcgatggaga ccaggatggc caccacgccc
      181 agcccgaccg ccgtgatccg ggccaccttg acctcctcct gctcgtcctc gacgccgttg
      241 cgcttgacgc ccgtccacag gtcgtgggcc acggcccccg aggcggcgat gaccagcctg
      301 gccaccacgg ccaggatcgt ggcgaaggcc accgcgctga tgaaggccag gaacaggtcg
      361 ccgccggtgg tgccagcgcc gccgcccagc tcctcggcca gcagcggcgt cgccaggttg
      421 cccccggcgc tggcctcctc ggcgcccgcg cccaggatgg cccgggcgcc cacgcccacg
      481 atcatcgtca tcaggaagaa cgcgccgatc agcgccacgg cccaggcgat ggacttccgc
      541 gccgccgcgg agtcgggcac ggtgaagaac cgcatcagga tgtggggcag cccggcggtg
      601 cccagcagca gcgccaggcc gaacgagacc gagctccacg ccgacgggtt ctgctggccc
      661 ggcgacaggt aggcgtcggc gcccttgccg tactcgcccg ccgcgcggtc gaacagctcg
      721 gtggggctga aggacacctc gttgagcacc aggatcgtca gcaccaggcc cgcggccatg
      781 agcagcccgg ccttgatgat ctggacccag gtggtggcca gcatgccgcc ggccacgacg
      841 tacacgacca tgaaggtgcc ggtgatcagc accgagaaca cgaagtcgat cccggccagc
      901 gcctggagca gcacgcccgc gcccaccatc tgggcgacca ggtagatgaa cgacacgacc
      961 agcgtgccca tggccgccgc gctgcgcgcg gggcgcgcgt tcaggcggaa ggccagcacg
     1021 tcggccatcg tgaacttgcc cgagttgcgc atgcgctcgg ccagcaggag caccaccggc
     1081 aggaaggacg cgccggccgc ggccaggccg acccagccgt cgacgccgaa caggaagatc
     1141 aggcccgcga agcccaggaa ggtggaggcc gagaggtagt cgcccgccat ggcgaagccg
     1201 ttctgcaggc cggagatgcc gcggcccgcg gcccagaact cggtggccgt gctggtgcgc
     1261 ttggacgccc agtaggtgat gcccagcgtg atcgccagga cgatcgcgaa gacgaccagc
     1321 gccagggtgt tggtgccgtc gccgatggcc aggagcgtca tgagcgcgcc tcgcccgagg
     1381 ttgcggcggc ctggcggatg cgctcctcca tgggcgacca cacgcgcgcc gagcggcgca
     1441 tgtagaggaa ggtgatgagc caggtcagca ggatcagcgc gaccccgccg acgaaggcga
     1501 gcgagatcga gccgatgacc tcggtcccca tgaagtcgcg ggcgaacccg gcgaggaaga
     1561 tgtagagcag cccggcgccg atgccgatgc tcccggccac ggccgagaac cgccggcggc
     1621 ccttggtcag ctcgcggaac tccgcggacc cctcgatggc cgtccagtcg aacgtctcac
     1681 tcatctctct ccatcctccg gttgcccggt cgcgggtacc cgccggtgcg ccagctcaag
     1741 cgtcgaccgt gcccgacccg ggcaagggaa gacccatgcc caccgatccg atgccctacg
     1801 agcacctcgg cgaagccctg gccaccgact acttcttcgt gcgcgagcag ttcgacgacg
     1861 agcagtgggc gcacttcatc gagacgcgcc ggttcgtgga caccgaggtg ctccccgcga
     1921 tcaacgacta ctgggtggcg gaggagatgc cgtgggacct gatccggcgg ctgcccgagc
     1981 tgggcctggt cggcgaggac atccagggct acggctgccc gggcatgagc ccgctgaccc
     2041 gcgggctcgt gaacatggag ctgcaccgcg gcgacggcag ccttggcacc ttcctcggcg
     2101 tgcagtcggg cctggcgatg aagtcgatcg ccatgctggg ctcggaggag cagaaggaga
     2161 cgtggctgcc ggccatggcc gagctggaca aggtcggggc gttcgcgctg accgagcccg
     2221 agcacggctc ggactcggtg gcgctggaga ccagcgcccg gcgcgagggc gacgagtggg
     2281 tgctggacgg cgccaagcgc tggatcggca acgcctcggt ggccgacgtg accgtggtct
     2341 gggcgcgcga cgccgaggac gagcaggtca agggcttcct ggtcgagggc gacgcccccg
     2401 gcttcacggc cacgaccatg accggcaagg gggcgtcgcg ggccatctgg caggcggaca
     2461 tcacgctcga gggcgtgcgc gtgcccgagg agatgcgcct gcccgaggcc gcgtcgttca
     2521 agcagacggg cgccgtgctg atgtccacgc gcacgacctg cgcgtggtcg gcgctgggtc
     2581 acgcggtggc cgcctacgac gccgccctgg cctacgccgg ccagcgcgag cagttcggca
     2641 agccgctcgt gggcttccag ctgatccagg accggctggt gcggatgctg tccgaggtca
     2701 cggcgatgca gctctactgc atgcagctcg cgcggctcga ggaggccgac cggctgaccc
     2761 cgaccatcgc cgggctggcc aagctgcaca acacgcgcaa ggcgcgcggc gtgatcgccg
     2821 aggcccgcga cctgatgggc ggcaacggcc tgctgctgga gaaccacgtg atccggcaca
     2881 tgggcgacat cgaggtgatc cacacctacg agggcacgga gacgatgcag tcgctgatcg
     2941 tgggccggga catcacgggc gtggcggcct tcacctgagc accttcccct gagcagccaa
     3001 aaccgactgg ccagtcagcc agtcgcgttt tcaggcagaa tgggcgtacc caccttccga
     3061 ttcgagagga cgtcccgacc aatggctgaa gcgctggtct tcgatgcgat ccgcaccccc
     3121 cgtggcaagg gcaagagcaa cggctcgctg cacaccacca agcccgtcga cctcgtggtc
     3181 ggcctgatgc acgagacgct gggccgcaac gtccagctcg acccctcgaa ggtggacgac
     3241 gtggtcctcg gcgtggtctc ccccgtcggc gaccagggcg cggacatcgc gaagacggcc
     3301 gccatcaagg ccgggctgcc cgacaccgtc gccggcgtgc agctcaaccg cttctgcgcc
     3361 tccggcctcg aggccgtgaa catcgccgcg cagaaggtcg cctcgggctg ggaggacctg
     3421 gtcttcgccg gcggcgtcga gtcgatgtcg cgcgtgccca tgggctccga cggcggcgcc
     3481 tgggccatgg accccgagac caactacgac acgtccttca tcccccaggg cgtgggcgcc
     3541 gacctgattg ccaccgtcga ggagttctcg cgcgacgacg tcgacgccta cgcggcccgt
     3601 tcgcaggagc gcgcggcggc ggcccaggcc gagggccggt tcgacaactc ggtgatcccg
     3661 gtcaaggaca tcaacgacca gg
//
LOCUS       DASUPW010000561         2749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_267850, whole genome shotgun sequence.
ACCESSION   DASUPW010000561 DASUPW010000000
VERSION     DASUPW010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267850"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="VFY44_12635"
     CDS             complement(<1..772)
                     /locus_tag="VFY44_12635"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX5911331.1"
                     /translation="MKVGIVGLPNAGKSSLFNALTRAGAQAANYPFTTVEPNVAIVPV
                     PDDRLDAVVDTVKATPTVYETMEFHDIAGLVRGAHEGEGLGNKFLANIRETDALVHVV
                     RAHDDEQVVHPEGKVDPGTDVETIETELLYADLEQAERRLEKVAREAKGGDKAKVAEE
                     GWLRELVAALGEGKPARTVPVPAAAPDALRNLAPLTSKPVLYLANVGEGDPLVVPEAL
                     AEHADRAGARAAAVSARIEAELSELDDEEEAAAMREDLG"
     gene            complement(769..1254)
                     /locus_tag="VFY44_12640"
     CDS             complement(769..1254)
                     /locus_tag="VFY44_12640"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5911332.1"
                     /translation="MLRRLDLGLLTLLRTRGHQPAVERVVLAFTRAGEHGVLWHLVAV
                     AGAVAHARERDRYVRAVRVVFLAYLGNIAMKHVVRRARPLLEELPALSPTVTQLSYPS
                     AHSTTSFAAAAALRGALPAPPLYAAAGAMALSRVYVGVHYPTDVAAGAVFGTALGELI
                     P"
     gene            complement(1263..1481)
                     /locus_tag="VFY44_12645"
     CDS             complement(1263..1481)
                     /locus_tag="VFY44_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911333.1"
                     /translation="MADLRSMDVGFEGGPVLAVRATEESYTNLVKALGDDAADRWQSI
                     TTDDSEILVDLSKVVYVRRESGEQKVGF"
     gene            complement(1491..1901)
                     /gene="mce"
                     /locus_tag="VFY44_12650"
     CDS             complement(1491..1901)
                     /gene="mce"
                     /locus_tag="VFY44_12650"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX5911334.1"
                     /translation="MFGEIDHIGIAVEDLDEAVALYQERLGMAHQHRERVEEFGVEAV
                     LLEIGDAHIELLTPTQPDSGVAKFLEKNGPGMHHVAYRTDDIDATLERLRGAGLRLID
                     EQPRTGIRDSRVAFLHPKSTGGVLTEIVQPAPGH"
     gene            complement(1921..>2749)
                     /locus_tag="VFY44_12655"
     CDS             complement(1921..>2749)
                     /locus_tag="VFY44_12655"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HEX5911335.1"
                     /translation="GKPLAADTDLDLVARQTSGLTGADLANICNEAAIFAGRAHRETI
                     QTGDFQAALERVVAGMQSRRVITDHEKRVVAYHEAGHALCSEILPSVEKVHRISIVPR
                     GQALGYTLNLPAEDRYLKTKDELMDYLVVLLGGRVTEHLIFGQITTGASDDLRKVHEI
                     SRSMVTDYGMGSKIQSKQLPATDYSMADATRRLIDEEQQFIADQAHRRALSMVSEHRA
                     LLEALAARLLDNEVLERTDIDKLVAEHEGKVASPARLEPLNGGAERRVAASEPYVEP"
BASE COUNT          345 a          986 c          994 g          424 t
ORIGIN      
        1 cgcccaggtc ctcgcgcatg gccgcggcct cctcctcgtc gtccagctcc gacagctccg
       61 cctcgatccg cgccgagacg gcagccgccc gcgcgcccgc gcggtcggcg tgctcggcca
      121 gcgcctcggg caccaccagc gggtcgccct cgcccacgtt ggccaggtac agcaccggct
      181 tggaggtcag cggcgccagg ttgcgcagcg cgtccggcgc ggcggcgggc accggcaccg
      241 tgcgggccgg ctttccctcg cccagcgcgg cgaccagctc gcgcagccag ccctcctccg
      301 ccaccttggc cttgtcgccg cccttggcct cgcgggccac cttctcgagc cgccgctcgg
      361 cctgctcgag gtcggcgtag agcagctcgg tctcgatggt ctcgacgtcg gtcccggggt
      421 cgaccttgcc ctcgggatgc accacctgtt cgtcgtcgtg ggctcgcacc acgtgcacca
      481 acgcgtcggt ctcgcggatg ttcgccagaa acttgttgcc caggccctcg ccctcgtgcg
      541 cgccgcgcac cagcccggcg atgtcgtgga actccatcgt ctcgtacacc gtcggggtgg
      601 ccttcacggt gtcgaccacg gcgtccaggc ggtcgtcggg caccggcacg atggccacgt
      661 tgggctcgac cgtggtgaac gggtagttgg ccgcctgcgc gcccgcgcgg gtgagcgcgt
      721 tgaacagcga cgacttgccg gcgttgggca gcccgacgat gccgaccttc atgggatgag
      781 ctcgcccagc gcggtgccga acacggcgcc ggcggcgacg tcggtcgggt agtgcacgcc
      841 cacgtacacg cgcgagagcg ccatggcgcc ggccgcggcg tacagcggcg gcgcgggcag
      901 ggcgccgcgg agcgcggccg cggcggcgaa cgacgtggtc gagtgcgccg acgggtacga
      961 cagctgggtc acggtgggcg acaacgcggg cagctcctcc agcagcggcc gcgcgcggcg
     1021 caccacgtgc ttcatcgcga tgttgcccag gtaggccagg aacaccacgc ggaccgcgcg
     1081 gacgtaccgg tcgcgctcgc gcgcgtgtgc gaccgcgccc gccacggcga ccaggtgcca
     1141 gagcacgccg tgctcgccgg cccgcgtgaa ggccagcacc acgcgctcga ccgcgggctg
     1201 gtggccccgg gtgcggagga gggtgagcag gccgagatcg aggcggcgca gcacagaagg
     1261 ggctagaagc cgaccttctg ctcgcccgac tcgcggcgga cgtagacgac cttcgagagg
     1321 tcgaccagga tctccgagtc gtcggtggtg atgctctgcc agcggtccgc ggcgtcgtcg
     1381 ccgagcgcct tgaccaggtt ggtgtaggac tcctcggtgg cccgcacggc cagcacgggg
     1441 ccgccctcga atcccacgtc catgctcctg aggtctgcca tcgctgtctc ctagtggccc
     1501 ggggccggtt gcacgatttc ggtgagcacc ccaccggtgg acttggggtg caggaaggcc
     1561 acgcggctgt cgcggatccc cgttcggggc tgctcgtcga tcaggcgcag gcccgcgccg
     1621 cgcaggcgct ccagcgtggc gtcgatgtcg tcggtccgat aggccacgtg gtgcatcccg
     1681 ggcccgttct tctccaggaa cttggccacg ccgctgtcgg gctgggtggg cgtgagcagc
     1741 tcgatgtgcg catcgccgat ctccagcagc acggcctcga cgccgaactc ctcgacgcgc
     1801 tcgcggtgct ggtgggccat gcccagtcgc tcctggtaca gggccacggc ctcgtccagg
     1861 tcctcgacgg ctatgccgat gtggtcgatc tcgccgaaca tggcggagga ctctagaggg
     1921 ctacggctcc acgtacggct cggacgccgc gacccggcgc tcggcgccgc cgttgagcgg
     1981 ctccagccgg gccggggagg ccaccttgcc ctcgtgctcg gccaccagct tgtcgatgtc
     2041 ggtccgctcg agcacctcgt tgtccagcag gcgcgcggcc agtgcctcca gcagcgcccg
     2101 gtgctcggag accatggaca gcgcccggcg gtgggcctgg tcggcgatga actgctgctc
     2161 ctcgtcgatc agccggcgcg tggcgtccgc catcgagtag tcggtggccg gcagctgctt
     2221 ggactggatc ttgctgccca tgccgtagtc cgtgaccatg gagcggctga tctcgtgcac
     2281 cttgcgcagg tcgtccgagg cgcccgtggt gatctggccg aagatcaggt gctcggtgac
     2341 ccggccgccc agcaggacca ccaggtagtc catgagctcg tccttggtct tgaggtagcg
     2401 gtcctccgcg ggcaggttga gcgtgtagcc cagggcctgg ccgcgcggga cgatcgagat
     2461 ccggtggacc ttctcgaccg agggcaggat ctccgagcac agcgcgtgcc cggcctcgtg
     2521 gtaggccacc acgcgcttct cgtggtcggt gatcacgcgg cgcgactgca tgccggccac
     2581 cacgcgctcc agcgcggcct ggaagtcacc cgtctggatg gtctcccggt gcgcgcgccc
     2641 ggcgaagatg gcggcctcgt tgcagatgtt ggccaggtcg gcgccggtca agccgctggt
     2701 ctggcgggcc acgaggtcca ggtcggtgtc ggcggcgagc ggcttgccg
//
LOCUS       DASUPW010000562         2534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_681893, whole genome shotgun sequence.
ACCESSION   DASUPW010000562 DASUPW010000000
VERSION     DASUPW010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681893"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VFY44_12660"
     CDS             <1..466
                     /locus_tag="VFY44_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911336.1"
                     /translation="DLHDVPGAGTVAALAPAQLEACGLSPSRAVVMVKAAREVARGRV
                     RLDDPSRHEYDWRRLRAIRGIGSWTVEYLALHGQGRDDVLPHGDLAYIKLVGVLAGLG
                     RRATEDEVREYFAPYAPYAGLAGTALAHAAAGGAFGPSPSVRAGHRRLPLTA"
     gene            463..867
                     /gene="msrB"
                     /locus_tag="VFY44_12665"
     CDS             463..867
                     /gene="msrB"
                     /locus_tag="VFY44_12665"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX5911337.1"
                     /translation="MSESETKVTKTDQEWKSELDPDRYHVLREAGTEPPFTGAYTHSK
                     ASGMYKCGACGSDLFSSETKFDSGTGWPSFTDPVVSQAVELRDDNSLFMKRTEVLCAT
                     CGSHLGHVFPDGPGEGGQRYCINSLALDLDEQ"
     gene            complement(899..1846)
                     /locus_tag="VFY44_12670"
     CDS             complement(899..1846)
                     /locus_tag="VFY44_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911338.1"
                     /translation="MLKFSRLFALLALLAVGLLAGCGSDDESGGGSSGGTSEDPTALL
                     KKAMAAEVESGELKVAASFDVKGSSKVSGPVSVTLEGPFKSRGKEQLPLLDWDVSLSG
                     AGQNFSGGIVATEDNAFVKFRGQTYEVGKQLYEQFVRQQAQQTKSGPQSLKELGIDPS
                     AWLQDPKLSDGESIGGSATRKVTGQIDVEKMVDDVLKAAESPAVRKQLQSSGQSVPEV
                     SDADKQKVVDAVEKADLTVNVDDDDIARKVVLSATFKVPEGVDADGATGGSFELSYEL
                     PKVGGDVDITAPSDAKPLALLLQQLGLGAGMPGGGLKTQ"
     gene            complement(1854..>2534)
                     /locus_tag="VFY44_12675"
     CDS             complement(1854..>2534)
                     /locus_tag="VFY44_12675"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5911339.1"
                     /translation="VGAIVRKPLTSIISLPKGKVRSPRDLEGKTVGTAGIDYQAAFLQ
                     TILDRAGVDPSTVKQRNLGFNLVAPLVSGKVDATLGAFWNYEGVQLRQQKRRPRIIRV
                     DRAGVPTYDELVLVANADALERDGKQIRAFVGAVARGTNALERDPEEGIKGLLKANPD
                     LDPKLQRASVKVTLPLFLPAKGKPFGYQDPQEWQAFAGWMRDNRLTTKIPDARGAFDN
                     RFLPGGGL"
BASE COUNT          348 a          914 c          884 g          388 t
ORIGIN      
        1 cgacctgcac gacgtccccg gcgcgggcac cgtggccgcg ctggcaccgg cccagctcga
       61 ggcgtgcggg ctctcgccct cgcgggccgt ggtcatggtc aaggccgcgc gcgaggtggc
      121 ccgggggagg gtccgcctgg acgacccctc ccgccacgag tacgactggc ggcggctgcg
      181 cgccatccgc ggcatcgggt cgtggacggt cgaatacctg gccctgcacg gccagggccg
      241 cgacgacgtg ctgccccacg gcgacctggc ctacatcaag ctggtcggcg tcctggccgg
      301 cctggggcgg cgcgccaccg aggacgaggt gcgcgagtac ttcgctccat acgcgcccta
      361 cgccgggctg gccggcaccg cgctggcgca cgcggcggcc ggtggcgcgt tcggcccgtc
      421 gccctcggtg cgcgctggac accggcgcct accattgact gcatgagcga gagcgagacc
      481 aaggtcacca agaccgacca ggagtggaag agcgagctgg accccgaccg gtaccacgtg
      541 ctgcgcgagg ccggcaccga gccgcccttc accggcgcct acacgcactc gaaggccagc
      601 ggcatgtaca agtgcggggc ctgcggctcc gacctgttca gctcggagac caagttcgac
      661 tccggcaccg gctggcccag cttcaccgac ccggtggtca gccaggccgt ggagctgcgc
      721 gacgacaact cgctgttcat gaagcgcacc gaggtcctgt gcgccacctg cggctcgcac
      781 ctcggccacg tcttcccgga cggcccgggc gagggcggcc agcgctactg catcaacagc
      841 ctcgctctgg acctcgacga gcagtaggag ggccggcgaa gccggcccgt tctcagccct
      901 actgcgtctt caacccgccg cccggcatcc cggcgcccag gcccagctgc tgcagcagca
      961 gcgccagggg cttggcgtcg gaaggcgcgg tgatgtcgac gtcgccgccc accttcggca
     1021 gctcgtagct gagctcgaag ctgcccccgg tcgcgccgtc ggcgtccaca ccctcgggca
     1081 ccttgaaggt ggccgacagg acgaccttgc gggcgatgtc gtcgtcgtcc acgttcacgg
     1141 tcaggtcggc cttctcgacg gcatcgacca ccttctgctt gtcggcgtcc gagacctcgg
     1201 gaaccgactg gccgctgctc tggagctgct tgcggaccgc ggggctctcg gccgccttca
     1261 ggacgtcgtc gaccatcttc tcgacgtcga tctggccggt gaccttccgc gtggccgagc
     1321 cgccgatgct ctcgccgtcg ctgagcttcg ggtcctggag ccatgcggac gggtcgatgc
     1381 ccagctcctt cagcgactgc gggccgctct tggtctgctg ggcctgctgg cggacgaact
     1441 gctcgtagag ctgcttgccg acctcgtagg tctgcccgcg gaacttcacg aacgcgttgt
     1501 cctcggtggc gacgatgccg ccggagaagt tctggcccgc gcccgagagg ctgacgtccc
     1561 agtccagcag cggcagctgc tccttgccgc gtgacttgaa cggtccctcg agcgtgacgc
     1621 tgacgggccc ggagaccttg gacgagccct tgacgtcgaa cgacgcggcg accttcagct
     1681 cacccgactc gacctcggcg gccatggcct tcttcagcag cgcggtgggg tcctcggagg
     1741 tgccgcccga cgagccgccg cccgactcgt cgtcgctgcc gcagccggcc aggaggccga
     1801 cggcgagcag ggccagcagg gcgaacagtc gagagaactt gagcatcagc gggttatagc
     1861 ccaccgcccg gcaggaagcg gttgtcgaac gcgccgcggg cgtcggggat cttcgtcgtc
     1921 aggcggttgt cccgcatcca gcccgcgaag gcctgccact cctgggggtc ctggtagccg
     1981 aacggcttgc ccttggcggg caggaacagc ggcagggtga ccttgaccga ggcccgctga
     2041 agcttggggt cgaggtcggg attggccttc agcagcccct tgatgccctc ctcggggtcg
     2101 cgctcgagcg cgttggtgcc ccgggccacg gcgcccacga aggcgcggat ctgcttcccg
     2161 tcgcgctcca gcgcgtcggc gttggccacc aggaccagct cgtcgtaggt gggcaccccg
     2221 gcgcggtcga cgcggatgat gcgcggcctg cgcttctgct ggcgcagctg cacgccctcg
     2281 tagttccaga acgcgcccag cgtggcgtcg accttcccgc tgaccagcgg cgcgaccagg
     2341 ttgaagccca ggttgcgctg cttgaccgtc gagggatcca ctccggcgcg gtccaggatc
     2401 gtctgcagga acgcggcctg gtagtcgatc ccggcggtgc ccacggtctt gccctccagg
     2461 tcacgcggcg agcggacctt gcccttgggc agcgagatga tcgaggtcag cggcttgcgc
     2521 acgatggcgc cgac
//
LOCUS       DASUPW010000563         4600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_544404, whole genome shotgun sequence.
ACCESSION   DASUPW010000563 DASUPW010000000
VERSION     DASUPW010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544404"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="VFY44_12680"
     CDS             <1..977
                     /locus_tag="VFY44_12680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX5911340.1"
                     /translation="TSDDDVSAARRADYAPEGAGTGSGALATLKRTLTEFSEDNITDW
                     AAALTYYSVQAIFPAIIALVSIIGLIGGNLTQTLLDNVEKLAPGPAKDILVPAIENLS
                     SSKGAAGLAFIIGLALALNSASSYVAAFTRASNSIYDVEEGRPIWKLKPQQIGIVLVL
                     ILMLVLVTVMVAVSGPAAEEIGKVVGVGDTAVKVWDIAKIPVVLIVVALMFGFLYKTA
                     PNVKQPGAKFFTPGGFVALFIWIIASILFAIYVAKFATLNKTYGSMGGIIAFLIWIWI
                     TNLAILFGAEFNSELQRSQQREAGEPAPLDEPFLEPRDTTKLDKKAAK"
     gene            980..1330
                     /locus_tag="VFY44_12685"
     CDS             980..1330
                     /locus_tag="VFY44_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911341.1"
                     /translation="MAGGPVRRVAEAVEGSIDSAAGLPDFQRELLEHMRSMDEGIQSL
                     AREMSAVRDDIAAVRASLGPSAEDEFKAMNASMTRVETRIEHIDEQIPDPDAPSPIEK
                     AKDALLGDNDKAEE"
     gene            1333..1773
                     /locus_tag="VFY44_12690"
     CDS             1333..1773
                     /locus_tag="VFY44_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911342.1"
                     /translation="MASKKRKAAKSGFKAGKRKAARTPAGTAVFTAKALAGNQQARAE
                     LRDAYASARKAYKRSADRSGRPNIVTLLDDRKAKKEAGNALTSLRHALKIAEKRRQKP
                     RGGKGPAVLVLTAAGAGAGALIYKQRSSSEETAATTTNGAAATA"
     gene            complement(1777..2700)
                     /gene="ligD"
                     /locus_tag="VFY44_12695"
     CDS             complement(1777..2700)
                     /gene="ligD"
                     /locus_tag="VFY44_12695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX5911343.1"
                     /translation="MSDPAPTKAQAMKAVLTDERFSDPDWIYERKLDGVRCIAIRDGD
                     RVRLLSRNDLSMNDRYPEVVEALEAQECRRFAIDGEVVAFDHGQTSFSRLARRGRERV
                     AVFLYAFDITWLEGEDVRGLPLTERKRLLRGALSFGGALRLSAHRRENGEEYFAEACR
                     KGWEGLIAKRADSRYTSARSKDWLKFKCERGQELVIGGYTPPKGSRSVLGALLMGYFA
                     DGELHYAGKVGTGFDQGTLADLGERLAPLVRDGSPFAERLTGVAGATWVEPELVAEVG
                     FTEWTRHGRLRHPRFLGLRFDKPASKVVREG"
     gene            complement(2697..3182)
                     /locus_tag="VFY44_12700"
     CDS             complement(2697..3182)
                     /locus_tag="VFY44_12700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5911344.1"
                     /translation="MPGARFVIQQHAASSMHFDFRLEVEGVLASWAVPKGPSTDPRQK
                     RLAVQVGDHDLEYAGFEGVHGRGYGAGAVIVWDNGTYDVLSEGTMREALDAGHASFWL
                     HGHKLRGGYTLQRTSAGEKPQWLLIKRRDDEADARRNPVSTEPESVISGLTVEQMGER
                     G"
     gene            3248..3883
                     /locus_tag="VFY44_12705"
     CDS             3248..3883
                     /locus_tag="VFY44_12705"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5911345.1"
                     /translation="MELTPELIEVARPPSPRGVVIVLHGGASRRENMRVSPAQLSVLR
                     MIPIARRIARTGRGELAVFRLLNSRRGWDAHHTPVRDVEWALDRVAELLGPSLPVCLV
                     GHSLGGRAALMSAGAPQVRGAVALAPWVLPTDDPPGLDAGRLLIVHGDRDRIASPQRS
                     AALAERLRRRGQRVEFVSVPGGKHAMLGHHAEFTAPAAEFAAAVLREPVTA"
     gene            complement(3873..>4600)
                     /locus_tag="VFY44_12710"
     CDS             complement(3873..>4600)
                     /locus_tag="VFY44_12710"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX5911346.1"
                     /translation="KAARVGCSGWNYRDWRGDFYPEGVPAKRWLEAYAERFDTVEVNT
                     TFYRLAKRPAVERWVEQTPPGFTFTVKASRYMTHMKRLRDLEPALKRFYEPLRPLADA
                     GRLGPVLWQLPGDFERDDERLAGLLGLLPEGRHALEFRHPSWFAPAVLDRLRDHGVAL
                     VIGDHPERPFQRHELTADWTLVRFHWGRRGRRGNYSDSEIATWARRLAQWRRRADVYA
                     YFNNDWEGFAPRNALTLAARLRP"
BASE COUNT          674 a         1643 c         1657 g          626 t
ORIGIN      
        1 tgacgagcga cgacgatgtg agcgccgcgc ggcgcgcgga ctacgcaccc gagggagccg
       61 ggacgggctc cggcgccctc gcgaccctga agcggacgct gacggagttc agcgaggaca
      121 acatcaccga ctgggcggcg gcgctgacgt actactccgt ccaggcgatc ttcccggcga
      181 tcatcgcgct ggtgtcgatc atcgggctga tcggcggcaa cctgacccag acgctgctgg
      241 acaacgtgga gaagctggcc cccgggcccg cgaaggacat cctcgtgccg gcgatcgaga
      301 acctgtcgtc ctccaagggc gcggcggggc tggccttcat catcggcctg gcgctggcgc
      361 tgaactcggc gtcgagctac gtggccgcgt tcacgcgcgc ctcgaactcg atctacgacg
      421 tcgaggaggg gcggccgatc tggaagctca agccgcagca gatcggcatc gtcctggtgc
      481 tgatcctgat gctggtgctg gtcaccgtga tggtggcggt cagcgggccg gcggccgagg
      541 agatcggcaa ggtcgtgggc gtcggcgaca ccgcggtcaa ggtgtgggac atcgccaaga
      601 tcccggtcgt cctgatcgtg gtcgcgctga tgttcggctt cctctacaag accgcgccga
      661 acgtgaagca gccgggggcg aagttcttca ccccgggcgg tttcgtggcg ctgttcatct
      721 ggatcatcgc gtcgatcctg ttcgccatct acgtggccaa gttcgccacg ctgaacaaga
      781 cctacgggtc catgggcggc atcatcgcgt tcctgatctg gatctggatc accaacctgg
      841 cgatcctgtt cggggcggag ttcaactccg agctccagcg gtcccagcag cgcgaggccg
      901 gcgagcccgc gccgctggac gagccgttcc tggagccgcg cgacaccacg aagctggaca
      961 agaaggccgc gaagtagccg tggccggagg gcccgtacgg cgggtggcgg aggcggtcga
     1021 aggctcgatc gactccgccg ccggcctgcc ggatttccag cgcgagctgc tggagcacat
     1081 gcgctcgatg gacgagggca tccagagcct ggcgcgggag atgagcgccg tccgcgacga
     1141 catcgcggcg gtgcgcgcgt cgctgggccc gtccgccgag gacgagttca aggccatgaa
     1201 cgccagcatg acccgggtcg agacccggat cgaacacatc gacgagcaga tccccgaccc
     1261 cgacgcaccg tcgccgatcg agaaggcgaa ggacgcgttg ctgggcgaca acgacaaggc
     1321 tgaggagtag acatggcgag caagaagcgc aaggcagcga agagtgggtt caaggcgggc
     1381 aagcgcaagg cggcccgcac cccggctggc accgcggtgt tcaccgcgaa ggcgctggcg
     1441 ggcaaccagc aggcgcgcgc ggagctgcgc gacgcctacg cgtcggcccg caaggcgtac
     1501 aagcgcagcg ccgaccgcag cggccggccg aacatcgtga cgctgctgga cgaccgcaag
     1561 gccaagaagg aggccggcaa cgcgctgacc tccctgcgcc acgcgctgaa gatcgccgag
     1621 aagcggcgcc agaagccgcg cggcggcaag ggccccgccg tgctggtgct gaccgccgcg
     1681 ggagcgggcg ccggcgcgct gatctacaag cagcgcagct ccagcgagga gaccgcggcc
     1741 accacgacca acggcgccgc ggccaccgcc tgacggtcag ccctcccgca ccaccttcga
     1801 cgcgggcttg tcgaaccgca ggcccaggaa gcgagggtgg cgcaggcgcc cgtggcgggt
     1861 ccactcggtg aagccgacct cggccaccag ctcgggctcg acccacgtgg cgcctgcgac
     1921 cccggtcagg cgctcggcga acggcgaacc gtcccgcaca agcggggcca gccgttcgcc
     1981 gaggtcggcc agggtgccct ggtcgaagcc cgtgcccacc ttgccggcgt agtgcagctc
     2041 gccgtcggcg aagtagccca tcagcagggc gccgagcacg ctgcgtgagc ccttcggcgg
     2101 cgtgtagccg ccgatcacca gctcctggcc gcgctcgcac ttgaacttga gccagtcctt
     2161 ggaccgagcc gaggtgtagc ggctgtcggc ccgcttggcg atcagcccct cccacccctt
     2221 ccggcacgcc tcggcgaagt actcctcgcc gttctcccgg cggtgggcgc tgaggcgcag
     2281 cgcgcctccg aacgacagcg cgccgcgcag cagccgcttg cgctcggtca gcggcagccc
     2341 gcgcacgtcc tcgccctcga gccaggtgat gtcgaacgcg tacaggaaca cggccacgcg
     2401 ctcgcggccg cgccgggcca gtcgcgagaa gctggtctgg ccgtggtcga aggccaccac
     2461 ctccccgtcg atggcgaacc gccggcactc ctgggcctcc agcgcctcca ccacctcggg
     2521 gtagcggtcg ttcatcgaga ggtcgttgcg cgagagcagc cgcacccggt cgccgtcgcg
     2581 gatggcgatg cagcggacgc cgtccagctt gcgctcgtag atccagtccg ggtcggagaa
     2641 gcgctcgtcg gtgagcaccg ccttcatggc ctgtgccttc gtgggcgcgg ggtcgctcac
     2701 ccgcgctcgc ccatctgctc caccgtcagc ccgctgatga ccgactccgg ctcggtgctg
     2761 accgggttgc ggcgagcgtc ggcctcgtcg tcgcggcgct tgatgagcag ccactgcggc
     2821 ttctcgcccg cgctggttcg ctgcagcgtg tagccgccgc gcagcttgtg cccgtgcagc
     2881 cagaagctgg cgtggccggc gtccagcgcc tcgcgcatcg tgccctcgga gagcacgtcg
     2941 taggtcccgt tgtcccagac gatcaccgcc ccggcaccgt acccccgtcc gtgcaccccc
     3001 tcgaagccgg cgtactccag gtcgtggtcg cccacctgga ccgccagccg cttctggcgc
     3061 ggatcggtcg acggtccctt gggcaccgcc cacgaggcca gcacgccctc gacctccagc
     3121 cggaagtcga agtgcatgct ggacgcggcg tgctgctgga tcacgaagcg ggcgccgggc
     3181 acggacccgc aatacccgga atcggcgggg cgtgatcccg cggccgcccc tgcgacggac
     3241 ccccgtcatg gagctgaccc cggagctgat cgaggtcgcg cggccgccga gcccgcgcgg
     3301 cgtggtgatc gtcctccacg gcggcgccag ccggcgggag aacatgaggg tcagcccggc
     3361 ccagttgtcg gtgctgcgga tgatcccgat tgcccgccgc atcgcccgga ccgggcgcgg
     3421 cgagctggcg gtgttccgcc tgctgaactc acgccgcggc tgggacgccc accacacgcc
     3481 cgtgcgcgac gtggagtggg cgctggaccg ggtggccgag ctgctgggac cgtctctgcc
     3541 cgtgtgcctg gtcgggcact cccttggcgg gcgcgcagcc ctgatgtcgg ccggcgcgcc
     3601 tcaggtgcgc ggggcggtgg cgctggcgcc ctgggtgctg cccaccgacg atccgccggg
     3661 gctcgacgcc gggcgtctgc tgatcgtcca cggcgaccgc gaccgcatcg ccagcccaca
     3721 gcggtcggca gcgctggccg agcggctgcg ccggcggggc cagagggtgg agttcgtcag
     3781 cgtgcccggg ggcaagcacg cgatgctcgg ccaccacgcc gagttcaccg cgcccgcggc
     3841 ggagttcgcc gccgcggtgc tgagggagcc ggtcacggcc tgagccgtgc cgccagcgtg
     3901 agcgcgttgc gcggcgcgaa cccctcccag tcgttgttga agtaggcgta gacgtcggca
     3961 cgccggcgcc actgggccag gcgccgtgcc caggtggcga tctcgctgtc ggagtagttg
     4021 ccgcgccgcc cgcggcggcc ccagtggaag cgcaccaggg tccagtcggc ggtcagctcg
     4081 tggcgctgga aggggcgctc ggggtggtcg ccgatcacca gcgccactcc gtggtcgcgg
     4141 aggcggtcca ggaccgcggg cgcgaaccag ctggggtggc ggaactccag cgcgtggcgg
     4201 ccttcgggca gcaggcccag caggccggcc agccgctcgt cgtcgcgctc gaagtcgccg
     4261 ggcagctgcc acagcaccgg ccccagccgc cccgcgtcgg ccagcggccg cagtggctcg
     4321 tagaaccgct tcagcgcggg ctccagatcg cgcagacgct tcatgtgggt catgtagcgg
     4381 ctggccttga ccgtgaaggt gaacccgggc ggggtctgct cgacccagcg ctccaccgcc
     4441 gggcgcttgg ccaggcggta gaaggtggtg ttcacctcca cggtgtcgaa ccgctcggcg
     4501 taggcctcga gccaccgctt ggcgggaacg ccctcggggt agaagtcgcc cctccagtcg
     4561 cggtagttcc agcccgagca gcccacccgg gcggccttca
//
LOCUS       DASUPW010000564         5115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_959578, whole genome shotgun sequence.
ACCESSION   DASUPW010000564 DASUPW010000000
VERSION     DASUPW010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5115
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959578"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="VFY44_12715"
     CDS             complement(<1..1080)
                     /locus_tag="VFY44_12715"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX5911347.1"
                     /translation="MATRVQTVEEICLAARDAARELAAVDGATRAAALRAMADAIDAN
                     ADEILAANESDMEAGREEGLGAALMDRLALDPARLAGIAADVRTIAALPDPVGETLDG
                     HRLPNGLDVQRVRVPLGVVAVVYEARPNVTIDCSALCIKSGNAIVLRGSSMAANSNAA
                     LARVAASAVEEAGLPAGAISLVTGGDRDELRQLATQDGVVDLIIPRGGEGLKNALKEH
                     ATVPVMYAAAGNCHVYVDASADQDDALDIIVNAKVQRPGVCNAAETLLVHADAASTFL
                     PRALPALREAGVELRVDGRARAAAGPLSDSLIDAGEDDWGTEYHALILAVKVVDSTAD
                     AIAHVNRYGSGHSEAIVTGSTASSQA"
     gene            complement(1096..1491)
                     /locus_tag="VFY44_12720"
     CDS             complement(1096..1491)
                     /locus_tag="VFY44_12720"
                     /inference="COORDINATES: protein motif:HMM:NF040028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6220 domain-containing protein"
                     /protein_id="HEX5911348.1"
                     /translation="MTSAAAPAPKSQTGLQIHLLLVVLALACLVVQVYLAGRGAFGAS
                     SYDAHKNLGHVLEPIALVLIIATVAIPATRNKGDIIQAVAFLVLVVVQTLLAEAGASV
                     AALHPVNALIIIGLLAGMLAKDRRLLTGG"
     gene            1551..2135
                     /locus_tag="VFY44_12725"
     CDS             1551..2135
                     /locus_tag="VFY44_12725"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5911349.1"
                     /translation="MTETNDEQPLKQVTRRIGRTLRAHRTERGMSLGELARSAGLSKS
                     ILARIEADGGNPSVETLWRLSLALGVPLGTLLGEDEGPRVRRLPARAGEGMAGESGMT
                     GWLLHTEGRVRRTELFDLDFPAGTDHQGTPHLPGTEELVVCVSGRMRVGPLGEEQDLG
                     PGDAVVFAADVPHRYAASEDSQALDWILYLDTSA"
     gene            2132..3295
                     /locus_tag="VFY44_12730"
     CDS             2132..3295
                     /locus_tag="VFY44_12730"
                     /inference="COORDINATES: protein motif:HMM:NF015551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HEX5911350.1"
                     /translation="MRPGDGQAVGAGIVSGVVGFGGAFAVVLAGLRGVGASPAEAASG
                     LMVLSVLMGALTVVLAVRTRLPMAIAWSTPGAALLATAGVPDGGFATAVGAFLLCGAL
                     LTLAGLWPWLERTVTRIPRTLAAAMLAGVLLPLCLKPFAAVESAPELVLPVIAVWAVL
                     LLFARPWAVPVALVVAIAAVVVSEPLDLGPASDLAPRLDLVAPDLRIGPILALAAPLF
                     IVTMASQNLTGMAVLTSFGFKPRWRTAVTSTGVATVAAAPFGGHALNLAAITAALVAG
                     PEAHPEPGRRWVAAAANGVTCMVFGLSAAAASAFLAAAPPEVLATVAGLALLAALGPA
                     LATALAEPEEREAALLTFVVSASGVTLLSVTAAAWGLAAGLLLLGAQRYSKTK"
     gene            complement(3277..4377)
                     /gene="proB"
                     /locus_tag="VFY44_12735"
     CDS             complement(3277..4377)
                     /gene="proB"
                     /locus_tag="VFY44_12735"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX5911351.1"
                     /translation="MATVVVKLGSSIVADPRGEVRTEVIDPLCAEVAARHHAGDSVVM
                     VTSGAIARGMRIMNLPARPAAMDELQAASAVGQGPLYQVYEGLLAQREVPSAQVLLTF
                     FDMSARSHYVNARHTLSRLLEWRVVPVINENDTTTTDEISFGDNDFLAAQVAILLGAE
                     RLVLLTDTDGLYTADPALDPAAEPVTDVTSFEQLESLQIGFSASPLGSGGMRSKVVAA
                     EMATAAGIPATIARGTDAAQVRAALAGRPAGTRFHPRAERVSSFKLWLKYAKPVQGVL
                     SVDDGAARALTERGTSLLPVGITAVAGHFDAGDAVEVRAAADDSRPIGKGIVNYSSSE
                     LERARGMKSEKVREMFGRPAEEAVHRDYFVLE"
     gene            complement(4451..4861)
                     /locus_tag="VFY44_12740"
     CDS             complement(4451..4861)
                     /locus_tag="VFY44_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HEX5911352.1"
                     /translation="MGARPITVCVLVAALGLVPGCGGGGDGRKDAQQVVRDFVTAVNE
                     KDGETFCKEITTREYVEQVTVAKGDAAVKQCETQIEQIRQPEFKLVKLVKTEVDGDRA
                     VVTAELEQQGRVRRQVFRMVKEDGGFRLTSGSTN"
     gene            complement(4884..>5115)
                     /gene="cgtA"
                     /locus_tag="VFY44_12745"
     CDS             complement(4884..>5115)
                     /gene="cgtA"
                     /locus_tag="VFY44_12745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HEX5911353.1"
                     /translation="FEVERVGDGEFRVTGDAVDRLIARHDLENDEALRYLEDRLRSMG
                     VIRALEAAGFTSGDDVEIAGVIFELDPGAPLR"
BASE COUNT          624 a         1834 c         1924 g          733 t
ORIGIN      
        1 ggcctgcgac gacgcggtcg agccggtcac gatggcctcg gagtggcccg agccgtagcg
       61 gttcacgtgc gcgatggcgt cggccgtgga gtccacgacc ttcacggcca ggatcagcgc
      121 gtggtactcg gtgccccagt cgtcctcgcc ggcgtcgatg agcgagtcgg acagcggtcc
      181 cgcggcggcg cgggcgcgcc cgtccacgcg cagctcgacc ccggcctccc ggagggcggg
      241 gagcgcccgc ggcaggaagg tggaggccgc gtcggcgtgc accagcagcg tctcggcggc
      301 gttgcacacg ccggggcgct gcaccttggc gttgacgatg atgtccagcg cgtcgtcctg
      361 gtcggccgac gcgtccacgt acacgtgaca gttgcccgcc gcggcgtaca tcacgggcac
      421 ggtggcgtgc tccttgagcg cgttcttcag gccctcgccg ccgcgcggga tgatcaggtc
      481 gaccaccccg tcctgggtgg ccagctggcg cagctcgtcg cggtcgccgc cggtgaccag
      541 cgatatcgct ccggccggca gcccggcctc ctcgaccgcg gacgccgcca cgcgggccag
      601 cgccgcgttg gagttggcgg ccatggacga cccgcggagc acgatcgcgt tgcccgactt
      661 gatgcacagc gctgagcagt cgatggtcac gttgggccgc gcctcgtaga ccaccgcgac
      721 cacgccgagc ggcacgcgga cgcgctgcac atccaggccg ttgggcagcc ggtggccgtc
      781 aagggtctcg cccaccgggt cgggcagcgc ggcgatggtg cgcacgtcgg cggcgatgcc
      841 cgccaggcgc gcggggtcca gcgccaggcg gtccatcagc gcggcgccga ggccttcctc
      901 gcggccggcc tccatgtcgc tctcgttggc ggccaggatc tcgtccgcgt tcgcgtcgat
      961 ggcgtccgcc atcgcacgca gcgccgcggc gcgggtggcg ccgtcgacgg cggccagctc
     1021 gcgcgcggcg tcgcgcgcgg ccaggcagat ctcctcgacc gtctgaaccc tggtggccat
     1081 ggaggccaag ggtacttagc cgcccgtcaa cagccggcgg tccttggcca gcatccccgc
     1141 cagcagcccg atgatgatga gggcgttcac cgggtgcagg gccgcgacgg acgcgccggc
     1201 ctcggccagg agcgtctgca cgacgaccag gaccaggaag gcgaccgcct ggatgatgtc
     1261 gcccttgttc cgcgttgcgg ggatggccac ggtggcgatg atcaggacca gcgcgatcgg
     1321 ctcgagcacg tggccgaggt tcttgtgcgc gtcgtagctg gaggcgccga aggcgccgcg
     1381 acccgcgagg tagacctgca cgaccaggca cgccagggcg aggaccacca gcaacaggtg
     1441 gatctggagg ccggtctggg acttgggagc gggagccgcg gcggatgtca tggcgcccaa
     1501 cgtacccgcg cccccggctg tcggccactg tgtattacga tggacgtccg ttgactgaga
     1561 cgaacgatga gcagcccctg aagcaggtga cccggcgcat cggccggacc ctgcgcgcgc
     1621 accgcaccga gcgcggcatg tcgctcggcg agctggcccg gtcggcgggc ctgtcgaaga
     1681 gcatcctggc ccgtatcgag gccgacggcg gcaacccgtc ggtcgagacg ctgtggcggc
     1741 tgtcgctggc gctcggggtc cccctcggca ccctcctggg cgaggacgag gggccacgcg
     1801 tgcgccgcct gcccgcccgc gccggcgagg gcatggcggg cgagtcaggg atgaccggat
     1861 ggctgctgca caccgagggc cgcgttcgcc gcaccgagct gttcgacctg gacttcccgg
     1921 cggggaccga ccaccagggc acgccccacc ttccggggac cgaggagctg gtggtgtgcg
     1981 tgagcgggcg catgcgggtg ggcccgctgg gtgaggagca ggatctgggc ccgggcgacg
     2041 ccgtcgtgtt cgccgccgac gtgccccacc ggtacgcggc cagcgaggac agccaggcgc
     2101 tggactggat cctgtacctg gacacgtcgg cgtgaggccg ggcgacgggc aggcggtcgg
     2161 agccgggatc gtctccgggg tcgtcggctt cggtggcgcg ttcgccgtcg tgctggccgg
     2221 gctgcgcggc gtcggggcgt ctccggcgga ggccgcctcc ggcctgatgg tgctgtcggt
     2281 gctgatgggc gcgctcacgg tcgtcctggc ggtgcgcacc cggctgccga tggcgatcgc
     2341 ctggtcgacg ccgggcgcgg cgctgctggc gaccgccggg gtgcccgacg gcgggttcgc
     2401 gaccgcggtg ggcgcgttcc tgctctgcgg cgcattgctg accctggccg ggctgtggcc
     2461 gtggctggag cggaccgtga cccggatccc ccggaccctg gcggcggcca tgctggccgg
     2521 ggtcctcctg ccgctgtgcc tgaagccgtt cgcggcggtc gagagcgccc ccgagctggt
     2581 cctgccggtg atcgcggtgt gggcggtgct gctcctgttc gcgcggccgt gggcggtgcc
     2641 ggtcgccctg gtcgtggcca tcgccgccgt cgtggtctcg gagccgctgg acctcggccc
     2701 cgcgtcggac ctggcgccgc ggctcgacct ggtcgccccg gacctgcgaa tcggcccgat
     2761 cctggcgctg gccgcgcccc tgttcatcgt cacgatggcc tcgcagaacc tgaccgggat
     2821 ggcggtgctg accagcttcg gcttcaagcc gcgctggcgc acggctgtga cctccaccgg
     2881 cgtggcgacg gtggccgccg cgccgttcgg gggccacgcg ctgaacctgg cggccatcac
     2941 cgccgcgctg gtggccgggc cggaggcaca cccggagccg gggcggcggt gggtcgccgc
     3001 ggccgcgaac ggcgtgacct gcatggtctt cggcctcagc gccgcggcgg cgagcgcctt
     3061 cctggcggcg gccccgcccg aggtcctggc cacggtcgcg ggcctggccc tgctggcggc
     3121 gctgggcccc gccctggcca ccgcgctggc cgagccggag gaacgcgagg ccgcgctgct
     3181 cacgttcgtg gtcagcgcgt cgggcgtgac gctgctcagc gtgaccgcgg cggcgtgggg
     3241 ccttgcggcg ggcctgctcc tcctgggagc gcagcgctac tccaagacga agtagtcgcg
     3301 gtgcacggcc tcctcggccg ggcgcccgaa catctcgcgc accttctcgg acttcatgcc
     3361 ccgggcccgc tccagctcgg aggagctgta gttcacgatc cccttgccga tcggccggct
     3421 gtcgtccgcg gccgcccgga cctccacggc gtcgccggcg tcgaagtggc cggccacggc
     3481 ggtgatgccc acgggcagca ggctggtgcc gcgctcggtc agcgcgcggg cggcgccgtc
     3541 gtcgaccgac aggaccccct gcaccggctt ggcgtacttg agccagagct tgaacgagga
     3601 cacgcgctcg gcgcgcggat ggaagcgggt gcccgcgggc cggcccgcca gggcggcccg
     3661 cacctgcgcg gcgtcggtgc cgcgggcgat ggtcgcgggg atgcccgccg cggtggccat
     3721 ctcggcggcc accaccttgg accgcatccc gcccgagccc agcggtgagg ccgagaagcc
     3781 gatctgcagc gactccagct gctcgaacga ggtcacgtcg gtcaccggct cggctgccgg
     3841 gtccagcgcg gggtcggcgg tgtagaggcc gtcggtgtcg gtcagcaaca ccagccgctc
     3901 ggcgcccagg aggatcgcca cctgagccgc caggaagtcg ttgtcgccga acgagatctc
     3961 gtccgtggtc gtggtgtcgt tctcgttgat gaccgggacc acgcgccact ccagcagcct
     4021 gctcagggtg tggcgggcgt ttacgtagtg gctgcgtgcg gacatgtcga agaacgtgag
     4081 cagcacctgg gcgctgggca cctcgcgctg ggccagcagg ccctcataga cctggtagag
     4141 cgggccctgg cccaccgcgg aggccgcctg gagctcgtcc atggccgcgg gacgggcggg
     4201 caggttcatg atccgcatgc cgcgcgcgat cgcccccgag gtgaccatga ccaccgagtc
     4261 gccggcgtgg tggcgcgcgg ccacctccgc gcagagcgga tcgatcacct cggtgcgcac
     4321 ctcgccgcgc gggtccgcga cgatgctcga gcccagcttg accaccacgg tcgccatggc
     4381 gagagagagt acggccctgc gggaggcgcg gagtccgatc gatgatcgga cgagcaccga
     4441 acgaggtttc tcagttcgtt gagccgctgg tcaggcggaa gccaccgtct tccttgacca
     4501 tccggaagac ctggcgacgg acccggccct gctgctcgag ctcggcggtc acgacggcgc
     4561 ggtcgccgtc cacctcggtc ttgacgagct tgacgagctt gaactcgggc tggcggatct
     4621 gctcgatctg ggtctcacac tgcttcacgg cggcgtcgcc cttggcgacc gtgacctgct
     4681 cgacgtactc ccgggtcgtg atctccttgc agaaggtctc gccgtccttc tcgttcacgg
     4741 ccgtgacgaa gtcgcgcacg acctgttgcg cgtccttcct gccgtcgccc ccgccgccac
     4801 acccgggcac gagccccagg gcggcaacga gcacgcacac cgtgatcggc cgggctccca
     4861 tgcgcgcgtc agggtaccgg ggctcagcgc agcggggccc cggggtcgag ctcgaagatc
     4921 accccggcga tctcgacgtc gtcgccggag gtgaagcccg cggcctccag cgcccggatc
     4981 acgcccatcg agcgcagccg gtcctccagg tagcgcagcg cctcgtcgtt ctcgaggtcg
     5041 tggcgggcga tcaggcggtc gaccgcgtcc ccggtgacgc ggaactcgcc gtcgccgacg
     5101 cgctcgacct cgaag
//
LOCUS       DASUPW010000565         5215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1514122, whole genome shotgun sequence.
ACCESSION   DASUPW010000565 DASUPW010000000
VERSION     DASUPW010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514122"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VFY44_12750"
     CDS             complement(<1..478)
                     /locus_tag="VFY44_12750"
                     /inference="COORDINATES: protein motif:HMM:NF013586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX5911354.1"
                     /translation="MRSRCLLACVLVLLAVPAAASAQSKVAPRGAFVSIQDVAPDVLL
                     DIRYRTAHNFIGSRIDGYREARCLVTRQTGAALARVQARVKAQGYTLKVYDCYRPQRA
                     VDQFVRWGKRLSDQKMKAEFYPRVRKSRVFKEGYIAEKSGHSRGSTVDLTLVKLPQR"
     gene            complement(509..2800)
                     /gene="lon"
                     /locus_tag="VFY44_12755"
     CDS             complement(509..2800)
                     /gene="lon"
                     /locus_tag="VFY44_12755"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX5911355.1"
                     /translation="METLLLIPVDDSVVFPNMTVTLTVDVGDEERVLIVPRKDDEFAS
                     VGTVAEVAEHVRLPGGGHAVALSGLHRAIAGAAHSSPSGELRVEVTPHPDDEPVDGKT
                     RNLEREYRAVVEELLELRGDDGRIAAFLRSITEPGTLADTSGYSPDISYEDKVDLLET
                     LDVTERLEKALDLQKQRLTELQVRQQIRDDVQSGADKQQREYFLRKQMDSIRKELDED
                     DASVAEEYRTKIEEAGMPDEVREQAEKELGRLERMGEQSGESSMIRTYLDWLIAVPWS
                     EESEEVLDPVKAREVLDADHAGLDDVKDRIVEHIGVMKLRRDRGIDDATDRKSGAILT
                     LIGPPGTGKTSIGESIARATGREFVRISLGGVRDEAEIRGHRRTYIGALPGRLVRALR
                     DAKTMNPVIMLDEVDKVGNDWRGDPSAALLEVLDPAQNHSFRDHYLDVELDLSKVLFI
                     ATANVSDTIPAPLLDRMEVVRFDGYTTEEKVAIARDYLWPRQRGRNGLREDEVTVDDA
                     VLRTVVSEYTREAGVRNLERELGTVLRKTATKIAGGTTEAPVAIDTDAIRDALGRQRF
                     FQEAAERTAVPGVATGLAYTPVGGDVLFIEATSMRGKDGLVLTGQLGDVMKESARIAL
                     SYVRGHADAVGAEEESFENREFHVHVPAGAIPKDGPSAGVTMTTALASLLSGRPVKHT
                     VGMTGEVTLQGRVLPIGGLKQKVLAAHAAGLTDVIIPERNRADIDEVPEEVRDAMTFH
                     PVMTIGEVLELALEPAQTTAGLA"
     gene            2951..3646
                     /gene="rpiA"
                     /locus_tag="VFY44_12760"
     CDS             2951..3646
                     /gene="rpiA"
                     /locus_tag="VFY44_12760"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEX5911356.1"
                     /translation="MTADPSPAREAASAAVVELVEDGMTVGLGSGRAVWRVAERLGER
                     FAGGAGSLRVAVASERTRERAREAGLSVVELDGTLELDLALDGADEIDPELRLIKGGG
                     GALLREKIVVAAARRFVVVAETPKKVDRLGEGFRLPVEVARFAWRDTRRRLAHVLPDP
                     ELRIREGDQEPYVTDEGHHILDCPIPPGEDLDALGDRLHRVPGVLEHGLFAGMAEQAV
                     LGTPEGGVELLRA"
     gene            3652..4143
                     /locus_tag="VFY44_12765"
     CDS             3652..4143
                     /locus_tag="VFY44_12765"
                     /inference="COORDINATES: protein motif:HMM:NF039927.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5946 family protein"
                     /protein_id="HEX5911357.1"
                     /translation="MGACPGCGLDLPDESGPTHAYMTSSPACWRVFGEVLAREFGDPA
                     WFTPHQITVDAYAAQHPGSPGRRERQSVALHLTTLGMVFERGFDPADGPKLHRRMAHR
                     PEYTWLKPPSMEGRLTVADVHAATDPAMHGDLVHRWGRDVWDAWSDHHATIWRWLDQA
                     QTP"
     gene            complement(4140..4469)
                     /locus_tag="VFY44_12770"
     CDS             complement(4140..4469)
                     /locus_tag="VFY44_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911358.1"
                     /translation="MEEAPENQKQGRGRIMAAAAAGVAMVAIALPVSGAFAEDSSSSS
                     SQRGTSVQQAPGFDDVQSGGSQGGDAQKRDCPKKDGQEGRSGSGSSGSDSSLTDPGAG
                     SPYAQQL"
     gene            complement(4673..>5215)
                     /locus_tag="VFY44_12775"
     CDS             complement(4673..>5215)
                     /locus_tag="VFY44_12775"
                     /inference="COORDINATES: protein motif:HMM:NF028286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcpC/CpaB family pilus assembly protein"
                     /protein_id="HEX5911359.1"
                     /translation="REVPSRFVPVDAVRNAQDLVGAPVQGGLRRGAYVTAGALGGAGA
                     PARGSPALRRGERAVQVAVAGGDPLGQAGGPGARVDVVVSTEPRDGAGRTFLALEDVE
                     LLSLGGSEGAARLGRAEGPGEGTGAAATASTTATLRVTQRQAVYLTAAQNFAREVRLL
                     PRPAGDARRAGRSAVSAAGL"
BASE COUNT          652 a         1934 c         1867 g          762 t
ORIGIN      
        1 gccgctgcgg cagcttgacc agcgtcaggt cgaccgtgga gccacgcgag tgccctgact
       61 tctcggcgat gtagccctcc ttgaacaccc gcgacttcct gacgcgcggg tagaactcgg
      121 ccttcatctt ctggtccgag aggcgcttgc cccagcgcac gaactggtcc accgcgcgct
      181 gcgggcggta acagtcgtag accttcagcg tgtagccctg ggccttgacg cgcgcctgga
      241 cccgggccag cgcggccccg gtctggcggg tgaccaggca gcgggcctcg cggtagccgt
      301 cgatccggct gccgatgaag ttgtgggccg tgcggtagcg gatgtccagc agcacgtcgg
      361 gcgccacgtc ctggatcgac acgaacgcgc ccctgggcgc caccttcgac tgcgcggagg
      421 cggcggcggg gacggccagg aggacgagga cgcaggcgag caggcaacgg gagcgcatgt
      481 cccgttgcct acccgtgtgt cccgcgcgtt acgccaggcc ggccgtggtc tgggccggct
      541 ccagcgccag ctccaggacc tcgccgatgg tcatgaccgg atggaaggtc atcgcgtcgc
      601 ggacctcctc cgggacctcg tcgatgtcgg cccggttgcg ctcggggatg atcacgtcgg
      661 tcagccccgc cgcgtgggcg gccagcacct tctgcttgag cccgccgatg ggcagcacgc
      721 ggccctgcag cgtgacctca ccggtcatgc ccaccgtgtg cttgaccggg cgcccgctca
      781 gcaacgacgc cagcgcggtg gtcatggtga ccccggcgct ggggccgtcc ttggggatgg
      841 cgccggccgg cacgtgcacg tggaactcgc ggttctcgaa ggactcctcc tccgcgccca
      901 cggcgtccgc gtgcccgcgc acgtaggaga gggcgatccg cgccgactcc ttcatcacgt
      961 cacccagctg gccggtcagg accaggccgt ccttgcccct catcgaggtg gcctcgatga
     1021 acagcacgtc gccgccgacc ggcgtgtacg ccagcccggt ggcgaccccg gggaccgccg
     1081 tgcgctcggc ggcctcctgg aagaaccgct ggcgcccgag ggcgtcgcgg atggcgtcgg
     1141 tgtcgatggc cacgggagcc tcggtggtgc cgccggcgat cttcgtcgcc gtcttgcgca
     1201 gcaccgtgcc cagctcacgc tcgaggttgc gcacgcccgc ctcgcgggtg tactcggaca
     1261 ccacggtgcg cagcaccgcg tcgtccacgg tcacctcgtc ctcgcgcagc ccgttgcgcc
     1321 cgcgctggcg gggccacagg tagtcacggg cgatcgcgac cttctcctcg gtggtgtagc
     1381 cgtcgaagcg cacgacctcc atccggtcca gcagcggcgc cgggatggtg tcggacacgt
     1441 tggccgtggc gatgaacagc accttgctca ggtccagctc cacgtccagg tagtggtcgc
     1501 ggaagctgtg gttctgggcc gggtccagca cctcgagcag ggccgcggac gggtcgcccc
     1561 gccagtcgtt gccgaccttg tcgacctcgt cgagcatgat caccgggttc atcgtcttcg
     1621 cgtcgcgcag ggcccgcacc aggcggcccg gcagggcgcc gatgtaggtc cggcggtggc
     1681 cccggatctc ggcctcgtcg cgcacgcctc ccagtgagat gcgcacgaac tcgcggccgg
     1741 tggcccgggc gatggactcg ccgatggacg tcttgccggt gccgggagga ccgatcaggg
     1801 tcaggatggc gcccgacttg cggtcggtgg cgtcgtcgat cccccggtcg cggcgcagct
     1861 tcatcacgcc gatgtgctcc acgatccggt ccttgacgtc gtccaggccc gcgtggtcgg
     1921 cgtccagcac ctcacgtgcc ttgaccgggt ccaggacctc ctcggactcc tcggaccacg
     1981 ggacggcgat cagccagtcc aggtaggtgc ggatcatcga gctctcgccc gactgctcgc
     2041 ccatgcgctc cagccggccc agctccttct cggcctgctc gcgcacctcg tccggcatgc
     2101 cggcttcctc gatcttggtg cggtactcct ccgccaccga ggcgtcgtcc tcgtccagct
     2161 ccttgcggat cgagtccatc tgcttgcgca ggaagtactc gcgctgctgc ttgtccgcgc
     2221 ccgactggac gtcgtcgcgg atctgctggc gcacctgcag ctcggtcagc cgctgcttct
     2281 ggaggtcgag ggccttctcc aggcgctcgg tcacgtccag ggtctccagc aggtcgacct
     2341 tgtcctcgta ggagatgtcc ggcgagtagc cggaggtgtc ggccagcgtg cccggctcgg
     2401 tgatcgagcg caggaaggcc gcgatccgcc cgtcgtcgcc gcgcagctcc agcagctcct
     2461 ccaccacggc gcggtactca cgctcgaggt tgcgggtctt gccgtccacg ggctcgtcgt
     2521 cggggtgcgg ggtgacctcg acgcgcagct cgccgctggg gctgctgtgc gcggcgcccg
     2581 cgatggcgcg gtgcaggccg ctcagcgcga ccgcgtgccc gcccccgggc aggcgcacgt
     2641 gctccgcgac ctcggccacc gtgccgacgc tggcgaactc gtcgtccttg cgcgggacga
     2701 tcaggacccg ctcctcgtca ccgacgtcga cggtcagcgt gaccgtcatg ttggggaaga
     2761 cgacggagtc gtcgaccggt atcagcagca gcgtttccat caggtcaact cgctctctct
     2821 gggtgcggac ttacgaaaga taagtagaac tatagcgccc tcagcgtccg tgtgagcaaa
     2881 cctgatgggc cgagacttag atcgtagtga tcggccgcat ccggccggga tgcgtggcag
     2941 gcttggcgcc gtgaccgccg acccctcccc cgcccgcgaa gccgcctccg cggccgtggt
     3001 cgagctggtc gaggacggga tgaccgtcgg cctgggatcg ggccgggccg tgtggcgggt
     3061 ggccgagcgc ctcggggagc ggttcgccgg cggcgccggc tcgcttcgcg tcgcggtggc
     3121 ttcggagcgc acgcgcgagc gcgcgcgaga ggccgggctc tcggtcgtcg agctggacgg
     3181 caccctggaa ctggacctgg ccctggacgg cgccgacgag atcgaccccg agctgcggct
     3241 gatcaagggc ggcggcggcg cgttgctgcg cgagaagatc gtcgtcgccg ccgcgcgccg
     3301 gttcgtggtc gtggccgaga cgccgaagaa ggtggaccgc ctcggcgagg gcttccggct
     3361 gccggtggag gtggcccggt tcgcctggcg cgacacgcgg cgccggctgg cgcacgtact
     3421 gcccgacccc gagctgcgga tacgcgaggg cgaccaggag ccctacgtga ccgacgaggg
     3481 ccaccacatc ctggactgcc cgatcccgcc cggcgaggac ctggacgccc tcggcgaccg
     3541 cctgcaccgc gttcccggcg tgctggagca cggcctgttc gccgggatgg ccgagcaggc
     3601 cgtgctcggc acgccggagg gcggggtcga gctgctgcgg gcctagccgg cgtgggggcc
     3661 tgtcccggct gcgggctgga tctgccggac gagagcgggc cgacccacgc ctacatgacc
     3721 tcgtcgcccg cgtgctggcg ggtgttcggc gaggtcctgg cacgcgagtt cggtgacccc
     3781 gcctggttca cgccgcacca gatcaccgtc gacgcctacg cggcgcaaca cccgggcagc
     3841 ccgggccgcc gcgagcgcca gtcggtggcg ctccacctga ccacgctggg gatggtgttc
     3901 gaacgcggct tcgaccccgc cgacggcccg aagctccaca ggcgcatggc ccaccgcccg
     3961 gagtacacct ggctgaagcc gccgtcgatg gagggccggc tcacggtggc cgacgtccac
     4021 gccgccacgg acccggcgat gcacggggac ctcgtccacc gctggggccg cgacgtgtgg
     4081 gacgcctggt ccgatcacca cgccacgatc tggcgctggc tggaccaggc gcagacgccc
     4141 tagagctgct gggcgtacgg gctccccgcg cccgggtcgg tcaggctcga gtcgctgccg
     4201 ctcgatccag agccgctccg gccctcttgg ccgtccttct tcgggcagtc gcgcttctgg
     4261 gcgtcgccgc cctgcgagcc gccggactgc acgtcgtcga agccgggcgc ctgctgaacg
     4321 ctggtgcccc gctgcgacga ggacgacgac gagtcctcgg cgaacgcccc cgacacgggc
     4381 agcgcgatgg cgaccatcgc cactccggcg gcagcggccg ccatgatccg cccgcgcccc
     4441 tgcttctggt tctcgggtgc ttcctccatc gtgcttcctt ccttcggggt acgcggacgc
     4501 aaggtaggca cgagcgtgtc acgcccacgt caccgttgtg tgtgaggtgc gtaagagccg
     4561 cgcacccgcg gtgcatttcg cccgcgtagc gagagaaatc gaccgcgcgg ggcccaggga
     4621 gacccggcgg ccaggggcac gggacgcgcg gccgacaggg gcgcccgtcg gctcaaagcc
     4681 ccgccgccga caccgccgac cgccccgccc ggcgcgcgtc gcccgcgggc cggggcagca
     4741 gccgcacctc gcgcgcgaag ttctgggccg cggtcaggta gacggcctgg cgctgggtca
     4801 cgcgcagcgt ggccgtggtg gaggcggtgg ccgcggcgcc ggtgccctcc cccggtccct
     4861 cggcgcggcc gaggcgcgcc gccccttccg atccgcccag cgacagcagc tccacgtcct
     4921 cgagcgccag gaacgtgcgc ccggcgccgt cccgcggctc ggtggacacg accacgtcga
     4981 cgcgggctcc cgggccgccc gcctggccca gcgggtcgcc gcccgcgacg gcgacctgga
     5041 cggcccgctc gcccctgcgg agcgccggcg acccgcgcgc cggggcgccc gccccgccca
     5101 gcgctccggc ggtcacgtac gcgccccggc gcaggccgcc ctggaccggg gcgccgacca
     5161 ggtcctgggc attccgcacc gcgtcgaccg gcacgaaccg cgacggcacc tcgcg
//
LOCUS       DASUPW010000566         2213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1652362, whole genome shotgun sequence.
ACCESSION   DASUPW010000566 DASUPW010000000
VERSION     DASUPW010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1652362"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..822)
                     /locus_tag="VFY44_12780"
     CDS             complement(67..822)
                     /locus_tag="VFY44_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911360.1"
                     /translation="MPDQDDRFGDLGEPRKPTAAERFEHEDRVNPEPDAPERRRPDVP
                     RPGNKYAWAVGVIALMVIGVVFFTVNLPNKGAGVRGPQAGERLPAFAAPLATSNVDDD
                     FDANVFPSPKEARSAGKRLSACEVRTESVLNSCELRERPAVLTMIATEGTDCEPQVDR
                     VERMRKAFPQVNFAVVMSGQKRSEAGRIVRRRGWGMPVGVDRDGAVTNLYGVGVCPTT
                     VFTDHRGRVRRTDLGNLTERQLRARARALLRRR"
     gene            complement(815..>2213)
                     /locus_tag="VFY44_12785"
     CDS             complement(815..>2213)
                     /locus_tag="VFY44_12785"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5911361.1"
                     /translation="AALVRLATSERGPRFPGVRSRARGGWERMADSGPGRAVAWRWAR
                     VRLWGIETSERALAHAVHRPRRILAVGFAVAVIGLAVDTQSEVVSDVRELVPQDLQAL
                     QDVNTLQKESGVAGEIDVVVRAKDITDPAVVAWMTKFQDRTLKAGGYKTGDSCSQAKD
                     PPDLCPALSLPDLFRTVDTRNAAATRGLLDSVPAYFSQGVITRDRTTANLAFGVRLQS
                     LADQKKIVESIEDRLDPPPGVRADVVGLPALAAEANSQLSSPLRRAFTLLAALAAVFF
                     VLFAFRRSAREAAVPLIPIALATGWASGLMFLLGLLPGPLSVKLNPMSVTLGALVIAI
                     STEFSVLLSARYRQERDAGSPPARAIELTYRSTGAAVMASGVTAIAGFAALIASDIRM
                     LRDFGILTVVDLGVSLAGVMLVLPAALVWAEQHGEFTLRDLNPRPLLAELGSAARDAG
                     SRLGLRRPRLRRRDA"
BASE COUNT          298 a          847 c          794 g          274 t
ORIGIN      
        1 cgggtagctc ctcggccagt tcctcctcca cccagccgtg ctccaggacg gggtcggcca
       61 cgcggctcac ctcctgcgca gcagcgcccg cgcacgggcg cgcagctgcc tctcggtcag
      121 gttgcccagg tcggtccggc gaaccctgcc gcgatggtcg gtgaacaccg tggtggggca
      181 cacgccgacg ccgtagaggt tggtcaccgc gccgtcgcgg tccacgccca cgggcatccc
      241 ccacccccgg cggcgcacga tccgcccggc ctcgctgcgc ttctggccgc tcatgaccac
      301 ggcgaagttc acctgcggga acgccttgcg catccgctcc acgcggtcca cctggggctc
      361 gcagtcggtg ccctcggtgg cgatcatggt cagcaccgcg ggacgctcgc gcagctcgca
      421 cgagttcagc accgactccg tgcggacctc gcaggccgac agccgcttcc cggcgctgcg
      481 ggcctccttg gggctgggga acacgttggc gtcgaaatcg tcgtccacgt tgctggtggc
      541 caggggcgcg gcgaaggcgg gcagccgctc gcccgcctgc ggcccgcgga cgccggcgcc
      601 cttgttgggc aggttcacgg tgaagaacac gaccccgatc accatcaggg cgatgacgcc
      661 gacggcccag gcgtacttgt tgcccggccg cgggacgtcg ggcctgcgcc gctcgggcgc
      721 gtcgggctcg gggttgacgc ggtcctcgtg ctcgaagcgc tcggccgccg tcggcttgcg
      781 cggctcgccc aggtcgccga agcggtcgtc ctggtcaggc atcgcgccgc ctcagccgcg
      841 ggcgccgcaa ccccagccgc gagccggcat cgcgggccgc cgagcccagc tcggccagca
      901 gcgggcgcgg gttgaggtcg cgcagggtga actcgccgtg ctgctcggcc cacaccagcg
      961 cggcgggcag gacgagcatc acgcccgcca gcgacacgcc gaggtccacc acggtcagga
     1021 tgccgaagtc gcgcagcatc ctgatgtcgg acgcgatcag cgcggcgaac ccggcgatgg
     1081 cggtcacccc cgaggccatg accgcggcgc cggtggagcg gtaggtcagc tcgatcgcgc
     1141 gcgccggcgg gctgcccgcg tcccgctcct ggcggtagcg ggcggacagc agcacgctga
     1201 actcggtgga gatcgcgatc accagggccc ccagcgtgac cgacatcggg ttgagcttca
     1261 ccgacagcgg cccgggcagc agccccagca ggaacatcag ccccgaggcc cagccggtgg
     1321 ccagcgcgat cgggatcaac gggacggccg cctcgcgcgc gctgcgccgg aaggcgaaga
     1381 gcacgaagaa caccgccgcc agcgccgcca gcagcgtgaa cgcgcgccgc agcggcgagg
     1441 acagctggga gttggcctcg gcggccagcg cgggcaggcc gaccacgtcg gccctgacgc
     1501 ccggcggcgg gtccaggcgg tcctcgatcg actccacgat cttcttctgg tcggccagcg
     1561 actgcagccg cacgccgaag gccaggttgg ccgtggtccg gtcgcgcgtg atcacgccct
     1621 gcgagaagta ggccggcacc gagtccagca gcccgcgcgt ggccgcggcg ttgcgggtgt
     1681 cgacggtgcg gaacaggtcc ggcagcgaca gggccgggca caggtcgggc gggtccttgg
     1741 cctgcgagca gctgtccccg gtcttgtacc cgcccgcctt gagcgtgcgg tcctggaact
     1801 tggtcatcca ggccacgacc gccggatcgg tgatgtcctt ggcccgcacc accacgtcga
     1861 tctcgccggc cacgccgctc tccttctgca acgtgttcac gtcctggagc gcctggaggt
     1921 cctggggcac cagctcgcgc acgtcggaga ccacctcgct ctgggtgtcc accgccaggc
     1981 cgatgacggc caccgcgaag cccacggcca ggatccggcg cgggcggtgc accgcgtgcg
     2041 ccagcgcccg ctcggaggtc tcgatgcccc acagccgcac ccgcgcccag cgccaggcca
     2101 cggcccggcc cggcccggag tcggccatcc gctcccagcc gccgcgcgcg cgggagcgca
     2161 cgcccgggaa gcgcggcccg cgctcgcttg tcgcaaggcg caccagcgcg gcg
//
LOCUS       DASUPW010000567         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_959851, whole genome shotgun sequence.
ACCESSION   DASUPW010000567 DASUPW010000000
VERSION     DASUPW010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959851"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1441
                     /locus_tag="VFY44_12790"
     CDS             122..1441
                     /locus_tag="VFY44_12790"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5911362.1"
                     /translation="MALGLLTVTACSGAEAGAEGGGTSNAAAEPAEIRLWLNGTDTPQ
                     ALRDYLIETFAEENEGSTLVIEEQDWNGLVPRLQTALTSEEQTPDLVEIGNTQSPTFT
                     YAGAFADISDMYDELGGDDLLSGFVEAGTADGTFYAAPYYSGARAVFYRKDFFEAAGV
                     EVPTTLAEFAEVATTLQEANPGGAANFSGFWFPGQDWYNGAAWIYTHGGDLAVQEDGE
                     WNGALSSPESLEALAEVQTLFTDATSAPRDADSNEPWIPFNNGESAMFSAPTWARWSI
                     DLPQCNQGVDPEDQSEEATAARAEQQACNEELTGVFPLPGMDEGQPATVFAGGSNIAI
                     PAMSQHQDLARNLLRIIFSEEFQVMLAENGLIPGNSQYGDALGDDVYAQAALAAAVDA
                     KLTPPAEKWADVEGARILEDFFQQVASGADLEEAAAEADQLIADTLN"
     gene            1515..>2087
                     /locus_tag="VFY44_12795"
     CDS             1515..>2087
                     /locus_tag="VFY44_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX5911363.1"
                     /translation="MTAPRTVTAEAVPPAPPRAPVPAATPQPPTRRIPLGRSLRSRFG
                     PYLLLVPALVPVLVGLGYPLARQFVLSFQEYGLAQQFGRPAEWVGLRNYVELLTDPYV
                     WGVIARSIAFCVVNAALTMVVGVALAVLMTKVGRAARVTLQVGMLLAWGTPVLATMTV
                     WQWLFDSQYGVINWLLAEPLGAEGMRNHSWL"
BASE COUNT          318 a          760 c          731 g          278 t
ORIGIN      
        1 gacgaccccg agccgaccag ttgcaggtcg gagaaccacc cacaggcggc gctgtccggc
       61 accgtccgca ccgggaagga acccaaccat gacgatcgta cggagaggtg gagtggccgt
      121 catggcactc ggcctgttga cggtcaccgc gtgcagcgga gcggaggcag gcgccgaagg
      181 gggcgggacc tcgaacgccg ccgccgaacc cgccgagatc cggctctggc tcaacggcac
      241 cgacaccccg caggcgctgc gcgactacct catcgagacc ttcgcggagg agaacgaggg
      301 ctcgacgctg gtcatcgagg agcaggactg gaacggcctg gtgccgcgcc tgcagacggc
      361 gctgacgtcg gaggagcaga cgcccgacct ggtggagatc ggcaacaccc agagcccgac
      421 cttcacctac gccggcgcct tcgccgacat cagcgacatg tacgacgagc tcggcggcga
      481 cgacctgctg tccggcttcg tcgaggccgg caccgccgac ggcaccttct acgcggcgcc
      541 ctactactcc ggtgcccgcg cggtgttcta ccgcaaggac ttcttcgagg ccgccggcgt
      601 ggaggtgccg acgacgctcg cggagttcgc cgaggtcgcg accaccctgc aggaggccaa
      661 ccccggtggg gcggccaact tctccgggtt ctggttcccc ggccaggact ggtacaacgg
      721 cgccgcctgg atctacacgc acggcggtga cctggccgtg caggaggacg gcgagtggaa
      781 cggggcgctg tcgagccccg agtccctgga ggcgctggcc gaggtccaga ccctcttcac
      841 cgacgcgacc agcgcgccgc gcgacgccga ctccaacgag ccgtggatcc cgttcaacaa
      901 cggtgagtcc gccatgttct ccgcgccgac ctgggcccgg tggagcatcg acctgccgca
      961 gtgcaaccag ggcgtcgacc ccgaggacca gtccgaggag gcgacggcgg cccgcgccga
     1021 gcagcaggcc tgcaacgagg agctgaccgg cgtcttcccg ctccccggca tggacgaggg
     1081 gcagccggcc acggtgttcg cgggcgggtc caacatcgcc atcccggcca tgtcgcagca
     1141 ccaggacctg gcgcggaacc tgctgcggat catcttcagc gaggagttcc aggtgatgct
     1201 ggccgagaac gggctgatcc ccgggaacag ccagtacggc gacgccctcg gtgacgacgt
     1261 ctacgcccag gctgccctgg cggccgcggt cgacgccaag ctgaccccgc cggccgagaa
     1321 gtgggccgac gtggagggcg cccggatcct ggaggacttc ttccagcagg tggccagcgg
     1381 agccgacctg gaggaggccg ccgcggaggc ggaccagctc atcgccgaca cgctgaactg
     1441 acctcccgac cgacacgtcg agggcgcggg ccgaccgtga gtccccgcgc cctcgacgca
     1501 caggagagcc cgacatgacc gctccacgca ccgtcacggc cgaggccgtg ccgccggcac
     1561 cgcctcgggc gccggtgccg gcggccacgc cgcaaccccc gacccgccgg atccccctgg
     1621 gcaggtcgct ccgctcccgt ttcggcccgt acctcctgct cgtcccggcc ctcgtgccgg
     1681 tgctggtcgg gctcggctat ccgctggccc gccagttcgt cctctccttc caggagtacg
     1741 gcctcgccca gcagttcggc cggccggccg agtgggtggg cctgcgcaac tacgtcgagc
     1801 tcctcaccga cccctacgtg tggggcgtca tcgcccggtc gatcgcgttc tgcgtggtca
     1861 acgcggcgct gacgatggtc gtcggggtgg cgctggcggt gctcatgacc aaggtcggcc
     1921 gcgccgcccg ggtcacgctg caggtgggca tgctgctggc ctgggggacg ccggtgctgg
     1981 ccacgatgac ggtctggcag tggctgttcg actcgcagta cggcgtcatc aactggctgc
     2041 tcgccgagcc gctgggcgcc gagggcatgc gcaaccactc gtggctg
//
LOCUS       DASUPW010000568         5371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1794938, whole genome shotgun sequence.
ACCESSION   DASUPW010000568 DASUPW010000000
VERSION     DASUPW010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1794938"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VFY44_12800"
     CDS             <1..351
                     /locus_tag="VFY44_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911364.1"
                     /translation="ERHDLTPLQLACQWNLAHPAVACCAPTLIQETGEHARPVEDKRA
                     ELAATPAAVTLSDAEVAAVREVGDNRGSMLLKGAAADHEGEEKPDRWALSDELAGVAE
                     RWGIDPARDLVKTA"
     gene            complement(348..1553)
                     /locus_tag="VFY44_12805"
     CDS             complement(348..1553)
                     /locus_tag="VFY44_12805"
                     /inference="COORDINATES: protein motif:HMM:NF039623.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5995 family protein"
                     /protein_id="HEX5911365.1"
                     /translation="MTALGAIFAVAALLALPSTAAAEDPPLLDWNPLLPGLPQTYQPS
                     KDADCVDGDPACIERTLTEMYQRFDANYASCDHNSAFGITYIRVTEAIRKAILDGVYQ
                     EPRFLAHEDRVFARMYFEAYDAWKAGKRDRVPAAWREAWDAGRAKSVTGLGNLLLSMN
                     AHINRDMPYLVEAIGMTKPDGSTRKIDHDRGNKVLNKLYDDVLKELSARYDSSIDDVD
                     VPGFLGDDAALFQMLQGWREGVWRNAELLDNAESAGQRKAASDYIEQYALTQARMIKS
                     FTTSPSPAGRDAQCAAYRRTHREAGGMARADKAKRGNRVSRKRRVKVRVRCPEVIRDC
                     RGRLALMRGKRTLGRTTLPVIRAGSSKVVTVKLARKLRRSTTVAAVAGSPSPWGTTRT
                     TVRGIRLKR"
     gene            complement(1624..3246)
                     /locus_tag="VFY44_12810"
     CDS             complement(1624..3246)
                     /locus_tag="VFY44_12810"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HEX5911366.1"
                     /translation="MLIRTTASIALIAGLLAVPSVAGAETVRVNGATGNNNGGNCGVT
                     PQPACKTIQFGAARAQGIAGADQILVAPGSYAEVVSVQGQGDTLAGAGSCDQAATCTI
                     IEPAPGVQDAVTSNVNNDNGQISGVRIKAATSMARIGLIMAGDNPVVRDVAVEMNAPN
                     NTKAALRLSGAGDAVVDRTTVTVTGDGDGFSPLAGLNSVTVTDSSVKTGTDYAMLIGA
                     GVGTVIRRSRFEAGVNANVVVSDDPGPVRLESSLIVGGNVGLAVGAPATVVGSTFDPG
                     QAGGSDGTAISTFYADAGETSTVRDSLLVGGIYTSGGQPVDCSNSVVGSAGTLAPTCV
                     AGTAGNSSADPAELFAGVAARDYRLKPGSPAVDAGSATALDPAEATDIAGNARVLDGN
                     GDCLARRDRGAYELTGFAAACPQGQQGATGGGTAAVPDTTKPTLARLAAGRTKLTFRL
                     SEAARVSVTVERKGRKGRRTVYRRVGGFSVGQAKAGANSAKVPVKLAKKLKAPATYRV
                     TLVATDAAGNRSAKGRKSFTVKPAKKKRRARR"
     gene            3375..3785
                     /locus_tag="VFY44_12815"
     CDS             3375..3785
                     /locus_tag="VFY44_12815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5911367.1"
                     /translation="MSQDATCTDAPAQDAAAERSLRVVLADDHGGYRIGLARAIAGHG
                     RLALAEAVRDGTAAVQAVARHKPDIALLDVRMPGLDGLETARRLRESGTAIILLSGHA
                     SPALTREAESAGAVALLSKELSRREICARLVELA"
     gene            3839..4480
                     /locus_tag="VFY44_12820"
     CDS             3839..4480
                     /locus_tag="VFY44_12820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5911368.1"
                     /translation="MPSDRVRVLVADDHPIYREGLVRSVKERPEFELVGEATDGRAAL
                     EAIRELEPDVAVLDLRMPDLGGVEVLNAIGRDDLATRVVLLSASVESEVVYRAVAMGV
                     GAYLSKEADRNAILDTVAAVARGETVLSPEIQSGLASQIQLRESSDRPVLTAREREIL
                     LLTADGRSAPDVAKELHLSTATVKTHLGSLYSKLGVSDRAAAVAVAMRAGLLE"
     gene            4477..>5371
                     /locus_tag="VFY44_12825"
     CDS             4477..>5371
                     /locus_tag="VFY44_12825"
                     /inference="COORDINATES: protein motif:HMM:NF020036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5911369.1"
                     /translation="MSVRHDDAPARYDETERVAGVGSFDYRPQENLTNWSLNLFRIYG
                     LDPEQAAPLDREAFSARIHPDDLGALDAMFADMARGGFYEADLRFRRFDDVERVVHVR
                     GESQLDAAGNLVRAHGTTQDVTESRATEAELRESRKRMVEAMRVAGVGSFEGRSDTGE
                     FEWSKELRGLYGLDPEGPHWTVPEYLERMVSDEVPAILEPDPPLGPFEFEHDVVRADG
                     ALRRYEVRGEVYPVDGVRWVRGTVRDVTAVRRGEAQQAAVATLTRQALEGVPLKRLLM
                     LACEHAASALGAESVGALELQP"
BASE COUNT          686 a         1925 c         1999 g          761 t
ORIGIN      
        1 gagcgccacg acctgacccc gctccagctg gcctgccagt ggaacctggc ccaccccgcc
       61 gtggcctgct gcgcgccgac gctgatccag gagaccggcg agcacgcgcg accggtcgag
      121 gacaagcgcg cggagctggc ggccacgccc gcggcggtca cgctctccga cgccgaggtt
      181 gccgccgtcc gcgaggtggg cgacaaccgc ggctcgatgc tgctcaaggg cgccgcggcc
      241 gaccacgagg gcgaggagaa gcccgaccgg tgggcgctct cggacgagct ggcgggcgtg
      301 gccgagcgct ggggcatcga ccccgcgcgc gacctcgtga agaccgctta gcgcttcagg
      361 cggattccgc gcacggtggt gcgcgtcgtg ccccacggcg acggcgagcc cgcgaccgcg
      421 gccacggtgg tggaccgccg cagcttgcgc gccagcttca cggtcacgac cttggagctc
      481 ccggcgcgga tgaccggcag cgtcgtccgc cccagggtcc gcttgccgcg catcagagcc
      541 aggcggccgc ggcagtcgcg gatgacctcg gggcagcgga cccgcacctt cacgcgccgc
      601 ttgcggctga cccggttccc gcgcttggcc ttgtcggccc tggccatgcc gcccgcctcg
      661 cggtgggtgc gccggtaggc ggcgcactgg gcgtcgcgcc cggccgggct ggggctggtg
      721 gtgaagctct tgatcatgcg cgcctgcgtc agcgcgtact gctcgatgta gtcggacgcg
      781 gccttgcgct ggcccgccga ctcggcgttg tccagcagct cggcgttgcg ccacacgccc
      841 tcgcgccagc cctgcagcat ctggaacagg gcggcgtcgt cgcccaggaa gccggggacg
      901 tccacgtcgt cgatcgagct gtcgtagcgg gccgacagct ccttgagcac gtcgtcgtag
      961 agcttgttca gcaccttgtt gccgcggtcg tggtcgatct tgcgcgtgga gccgtcgggc
     1021 ttggtcatgc cgatcgcctc gaccaggtag ggcatgtccc ggttgatgtg cgcgttcatg
     1081 gacagcagca ggttgcccag cccggtcacc gatttcgcgc ggccggcgtc ccaggcctcg
     1141 cgccacgcgg ccggcacccg gtcgcgcttg cccgccttcc acgcgtcgta ggcctcgaaa
     1201 tacatgcgcg cgaacacccg gtcctcgtgg gccaggaagc gcggctcctg gtagacgccg
     1261 tccaggatcg ccttgcggat ggcctcggtc acgcggatgt aggtgatccc gaaggccgag
     1321 ttgtggtcac agctcgcgta gttcgcgtcg aagcgctgat acatctcggt cagcgtgcgc
     1381 tcgatgcagg ccgggtcgcc gtccacgcag tcggcgtcct tggacggctg gtaggtctgg
     1441 ggcagcccgg gcagcagcgg gttccagtcc agcagcggcg gatcctcggc cgcggcagtc
     1501 gatggcaggg cgagcagcgc cgcgacggcg aagatcgccc ccagtgccgt cactccccta
     1561 cgcaatgcac gccccgccat ccaaccggcg attgtgccag acggaaccgg tgcgtaccag
     1621 cccctaccgg cgggcgcggc gcttcttctt cgccggcttg acggtgaagc tcttgcggcc
     1681 cttcgccgac cggttgcccg ccgcgtcggt ggccaccagc gtgacccggt aggtcgccgg
     1741 cgccttcagc ttcttcgcca gcttcaccgg gaccttggcc gagttcgccc cggccttcgc
     1801 ctgcccgacc gagaacccgc cgaccctgcg gtagaccgtt cgccgcccct tgcggccctt
     1861 gcgctcgacc gtcaccgaca ccctggcggc ctccgacagc cggaaggtca gcttggtccg
     1921 ccccgcggcc agccgggcca gggtgggctt cgtcgtgtcc gggaccgcgg ccgtgccgcc
     1981 cccggtggcg ccctgctggc cctgcgggca ggcggcggcg aagccggtga gctcgtaggc
     2041 gccgcggtcg cggcgggcga ggcagtcgcc gtttccgtcc agcacgcgcg cgttgccggc
     2101 gatgtcggtg gcctccgccg gatcgagcgc ggtggccgag cccgcgtcga cggcgggcga
     2161 gccgggcttg agccgatagt cgcgggcggc gacgccggcg aacagctccg ccgggtcggc
     2221 gctgctgttg cccgcggtgc ccgccacgca ggtgggcgcc agcgtgccgg cggacccgac
     2281 gacgctgttg gagcagtcca cgggctggcc gcccgaggtg tagatccccc cgaccagcag
     2341 cgagtcacgc accgtggacg tctcgccggc gtcggcgtag aaggtcgaga tcgcggttcc
     2401 atcgcttccc ccggcctgac ccgggtcgaa cgtcgagccc accaccgtcg cgggcgcccc
     2461 cacggccagg ccgacgttcc cgccgacgat cagggacgac tccagccgga cggggcccgg
     2521 gtcgtcgctg acgacgacgt tggcgttcac gcccgcctcg aagcgcgagc ggcggatgac
     2581 ggtgccgacc cccgcgccga tcagcatcgc gtagtccgtg ccggtcttga ccgacgagtc
     2641 ggtgacggtg acgctgttca gccccgccag cggggagaag ccgtcgccgt ccccggtgac
     2701 ggtcaccgtg gtcctgtcga ccaccgcgtc gccggcgccg gacaggcgaa gcgccgcctt
     2761 ggtgttgttg ggggcgttca tctcgaccgc gacgtcacgg acgacggggt tgtcgccggc
     2821 catgatgagg ccgatccgcg ccatgctggt cgcggccttg atccgcacgc cggagatctg
     2881 gccgttgtcg ttgttcacgt tcgaggtcac ggcgtcctgg acgccggggg cgggctcgat
     2941 gatcgtgcac gtggccgcct ggtcacagct gcccgcgccg gccagcgtgt cgccctgccc
     3001 ctggaccgac accacctcgg cgtagctgcc gggggcaacc aggatctggt ccgccccggc
     3061 gatgccctgg gcccgggccg ctccgaactg gatggtcttg caggcaggct gcggcgtcac
     3121 gccgcagttg ccgccgttgt tgttgccggt ggcgccgttc acgcgcaccg tctcggcgcc
     3181 ggccacggag gggaccgcca ggaggccggc gatcagggcg atggaagctg tggttcggat
     3241 gagcacgacg gccaaaatgc cagcgcctgc ccgggtgcgc agtctcactt cggtctcact
     3301 tcaggctcgg ttcagcccac ggttcctccg atcggatgag cacggacggt gccgcggtgc
     3361 agcagcatgg gcccatgtcc caggacgcca cgtgcacgga cgcaccggcg caggacgcgg
     3421 cggcggagcg ctcgctccgg gtcgtgctgg ccgacgatca cggtgggtac cggatcgggc
     3481 tggcgcgtgc catcgccggc cacgggcggc tcgcgctggc cgaggccgtg cgggacggga
     3541 cggccgccgt gcaggccgtc gcgcggcaca agccggacat cgcgctcctg gacgtgcgga
     3601 tgcccggcct cgacggcctc gagaccgcgc gccggctgcg cgagagcggg acggcgatca
     3661 tcctgctgag cgggcacgcc tcgcccgcgc tgacccgcga ggccgagagc gccggcgcgg
     3721 tggccctcct gagcaaagag ctgtcgcggc gtgagatctg cgcgcggctt gtggaactgg
     3781 cctgacccac ttgcgcgagt tgcgtttcag gggcacttgc ccctggcaga atcgccgcat
     3841 gccaagcgac cgcgtccggg tcctggtggc cgatgatcat ccgatctatc gggagggact
     3901 ggtgcggtcg gtcaaggagc gccccgagtt cgagctggtg ggcgaggcca ccgacggccg
     3961 cgcggcgctg gaggcgatcc gcgagctgga gcccgacgtg gccgtgctgg acctgcgcat
     4021 gcccgacctg ggcggcgtcg aggtgctgaa cgcgatcggc cgcgacgacc tggcgacgcg
     4081 cgtggtgctg ctgtcggcca gcgtggagag cgaggtggtg taccgcgccg ttgccatggg
     4141 cgtgggcgcc tacctgtcca aggaggccga ccgcaacgcg atcctggaca cggtggccgc
     4201 ggtcgcgcgc ggcgagaccg tgctctcgcc cgagatccag agcgggctgg cctcgcagat
     4261 ccagctccgc gagagctccg accgcccggt cctgaccgcg cgcgagcgcg agatcctgct
     4321 gctgaccgcc gacggccgct cggccccgga cgtggcgaag gagctgcacc tgtccaccgc
     4381 cacggtcaag acgcacctgg gcagcctcta ctccaagctc ggggtctccg accgggccgc
     4441 cgcggtcgcg gtggccatgc gggccgggtt gctggagtga gcgtgcgcca cgacgacgca
     4501 ccggcccgct acgacgagac cgagcgcgtc gccggggtgg gcagcttcga ctaccggccc
     4561 caggagaacc tgaccaactg gtccctgaac ctgttccgga tctacgggct cgaccccgag
     4621 caggccgcgc cgctggacag ggaggcgttc agcgcgcgga tccatcccga cgacctcggc
     4681 gcgctggacg cgatgttcgc cgacatggcc cggggcggct tctacgaggc cgacctgcgc
     4741 ttcaggcgct tcgacgacgt cgagcgcgtg gtgcacgtgc gcggcgagag ccagctggac
     4801 gccgccggca acctggtccg cgcccacggg acgacgcagg acgtgaccga gtcgcgggcg
     4861 accgaggccg agctgcgcga gagccggaag cgcatggtcg aggccatgcg cgtcgccggc
     4921 gtgggcagct tcgagggacg gtccgacacc ggggagttcg agtggtccaa ggagctgcgc
     4981 gggctgtacg ggctggatcc cgagggcccg cactggaccg tgccggagta cctggagcgc
     5041 atggtctccg acgaggtgcc cgcgatcctc gagcccgatc cgccgctggg gccgttcgag
     5101 ttcgagcacg acgtggtccg ggcggacggg gcgctgcgcc gctacgaggt ccgcggcgag
     5161 gtctacccgg tggacggcgt gcgctgggtg cgcggcaccg tgcgcgacgt gaccgcggtc
     5221 cgccggggcg aggcccagca ggcggcggtg gccacgctga cccgccaggc gctggagggc
     5281 gtgccgctca agcgcctgct gatgctggcc tgcgagcacg ccgcctccgc cctgggcgcc
     5341 gagagcgtgg gggcgctcga gctccagccc g
//
LOCUS       DASUPW010000569         7741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_132215, whole genome shotgun sequence.
ACCESSION   DASUPW010000569 DASUPW010000000
VERSION     DASUPW010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7741
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132215"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFY44_12830"
     CDS             <1..252
                     /locus_tag="VFY44_12830"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HEX5911370.1"
                     /translation="RPTATRVAANLTERGLVERAGDPRDGRVSLLSVTAEGHALLEEV
                     RSRKNAYLADRMADLGPEDRAALERAADVLERILEAEPR"
     gene            252..1568
                     /locus_tag="VFY44_12835"
     CDS             252..1568
                     /locus_tag="VFY44_12835"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5911371.1"
                     /translation="MFSLRGSLGQGPGLAPRASAALRKPFLSLSVPNYRRYFTGQVVS
                     LSGNWVQIVAETWLILRLTHSGVAVGLLAALQFAPMLLLGAYGGLLADRFDKRRLLML
                     TQLGMVVPALALFAVTATDVVAPWMVFALVLARGTVNALDNPARQSFVIEMVGPDRVV
                     NAVSLNSVIVHCARMVGPAIAGVLIATVGVEPCFLINAASFMAMLIALRRMDAAALAR
                     PDRAQRRPHALRDGLAYVARTPALAVPLAMMVLVGTLGFNFQVILPLLAKFTFDGGAG
                     TYAALVVAMGAGSVVGALTAGARGRVSPALLVGASAAFGLATVLAAVAPTLPLTVAAL
                     ALVGAASVTFAAGVNSTLQLEVAPEMRGRVMALYSVVFLGSTPIGGPLAGWVAQAASP
                     RAALMVGAAAALIAAGGAAVAGARGRKYRGTQGFHGRIEVGASTHG"
     gene            1616..2608
                     /locus_tag="VFY44_12840"
     CDS             1616..2608
                     /locus_tag="VFY44_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911372.1"
                     /translation="MPSTKSRSRTPARRRPGSRSQHDAPSPREHPFQWLTDQARSGLK
                     DLPANASWLLSRALEPAEAAGDAAGSAAHDTRDRARKVKASVKDAVPGGDSVEERMKR
                     AREAAERAQDAENDAVEAAREAKERSERAKEVGEQGRRHVAAVQKESADNAKGRVAEA
                     RRVADEKVAAEQAAAEADAEEEVRRAEQEAQEEAEAAQQEAEEAQANAKKQLEEANER
                     LAEARRLADEASAAAREAAEQAQRQAEQLAADADEQRRQADAKVEAAEAVRARADTDA
                     KTTTRQIKREQVNGDLKDRTKPELLDLAAAVEIEGRTTMTKDQLVKAIDKEAAR"
     gene            2605..2814
                     /locus_tag="VFY44_12845"
     CDS             2605..2814
                     /locus_tag="VFY44_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911373.1"
                     /translation="MNPFHRKSPLEKLGDSLGDVVKRIPKALESVPRPGRSGVAALGG
                     LAGLAAGSAALSSRRRRSEDSDGDA"
     gene            2811..3059
                     /locus_tag="VFY44_12850"
     CDS             2811..3059
                     /locus_tag="VFY44_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911374.1"
                     /translation="MTEPTLVRARVLARVLGVKLLTLEAQVVLEPAAAAPEPAALPPR
                     RGPTGEPRPIGVGLAQATRAIDEGEASLAAARNGVSGA"
     gene            complement(3046..3474)
                     /locus_tag="VFY44_12855"
     CDS             complement(3046..3474)
                     /locus_tag="VFY44_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911375.1"
                     /translation="MTAVVVLAAAGAGCGGDDEPGEPPRTVATTERTAPATTARTAPR
                     PAPLEPARCPAGAANCTSATGRVVYVEAVDPDGDGDAHFVLAGGDVTGPGLSVVDVRR
                     DLRPDPLPRPGDTVTAAGPVYRGSYGQRQIEARQVRVRRR"
     gene            complement(3489..3929)
                     /locus_tag="VFY44_12860"
     CDS             complement(3489..3929)
                     /locus_tag="VFY44_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911376.1"
                     /translation="MRAARTLICVAACLVALPAPARAARIDIDRATPLLRAGEPAQMR
                     VRLSFTGERTCHLTFKRGRLTQTTEDVLTEHRYVTWRWRVPKAARSGRWTLTAECGAE
                     AELTVRATRRLRLAGGFAGPRIAARRVNVFTSGDPLVVAFVERP"
     gene            complement(3956..5221)
                     /locus_tag="VFY44_12865"
     CDS             complement(3956..5221)
                     /locus_tag="VFY44_12865"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEX5911377.1"
                     /translation="MRHRRELMILAAVGAALSAPAPAGAAERVLVGFDKGVSKERQGK
                     IVGELGGRIEKRFAAVRGGRLVAVNARGGRRLDAVRKVLRKADGVTYAEPDFTLSTAA
                     TRTPNDPDYPFQYAVANSATGAISAPEAWGTRTSCSTVAVLDTGIDTDHPDLAPNIYK
                     SSDKPNNGKDDDKNGYVDDTYGLNVIKGKGSGEDDNGHGSHVSGIIGGRGNNGTGVSG
                     ICWSTKLMAVKFMNSKGKGSTSDAIDGIQYAAKKGAKVINCSFGSSSKSSALHDAVDY
                     AQSKGSLLVVAAGNDGDNIDKKPEYPASYTDSNILTVAATTSTDTLASFSNYGSTAVD
                     VAAPGNQILSTYLGGGYKLLSGTSMASPYAAGTAAMLRKQESGATYSDLRYAIRHKVD
                     TPPALKGKVAYNGRLNAQRALAAIGSLVD"
     gene            complement(5205..7514)
                     /locus_tag="VFY44_12870"
     CDS             complement(5205..7514)
                     /locus_tag="VFY44_12870"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5911378.1"
                     /translation="MIGLWLVLAVAIVAAARSAGSQTSDNLSLPGTGSTQSQDLLSSK
                     LPDQAYGSNPVVVHTDKGKLTDSAPRKAIEDTVTALGKVPGVIKATDPLQGSKNPALS
                     KDGQSAYISVFLSEGPADLTEDDAQKVVDASKPLSDAGLDTATGGYVGKAVSKPATES
                     SEVVGLTAAVVILLFAFGTATAMALPIVTAVLGLVISLSAIGLLGHALEVPSVGATLA
                     TMIGLGVGIDYALFIVTRHKIQLGEGMDMTESIGRATATAGGAVAFAGFTVVIALVSL
                     AFAGIPLVSALGYTAAVAVAVAVAAATTLLPALLGALGPRINSLRVQLGKTHPDDHEP
                     HGWRRWARGVAKRPATSIVAAVAVLAVLAAPVLNLHLGVADVAAAPKDTQQRQAYDLL
                     TLGFGVGQNGPLLIATQLGSPAKPDQKNLNELDDQQKQIDQQQQQAEAQGLPPDQAKQ
                     KTASQQQKLDDQKKKAENPATDARLTTLSDDVAKAPGIKSVTPPVLDKKADTAVFTAI
                     ATTAPADRKTETTVRELRDTTIPAAVKGTDLEAYVGGQTAGYIDLADRISDKLPSMIA
                     IVVGLSFIVLLLAFRSVLMPLKAGIANLLSVAAAYGVVTFVFQEGHGATLIGLDGATP
                     IASFVPLLMFAILFGLSMDYEVFLLTQIKEHYNESGDARQAVIDGLATTGRVITSAAL
                     IMVFVFSSFILNGDPTVKQFGVGLAVAIAIDATLVRCLLVPAVMVKLGAASWWLPRWL
                     AKALPEISIEGESFFAERDRKAAADASPA"
BASE COUNT         1016 a         2751 c         2917 g         1057 t
ORIGIN      
        1 cgcccgacgg ccacgcgtgt ggcggccaac ctgaccgagc gcggcctggt cgagcgggcg
       61 ggggacccac gcgacggccg ggtgagcctg ctctccgtga ccgccgaggg gcacgccctg
      121 ctggaggagg tccgctcgcg caagaacgcg tacctggcgg accgcatggc cgacctcggg
      181 ccggaggacc gcgccgcgct ggagcgcgcg gccgacgtgc tcgagcgcat cctggaggcc
      241 gagccgcggt gatgttctcg ctgcggggct cgctcggcca gggcccgggg ctcgctcctc
      301 gcgctagcgc ggcgctgcgc aagccgttcc tctcgctgag cgtgccgaac taccgccggt
      361 acttcaccgg ccaggtggtg tcgctgagcg gcaactgggt gcagatcgtg gccgagacgt
      421 ggctgatcct gcgcctgacc cacagcggcg tggcggtcgg cctgctggcc gcgctccagt
      481 tcgcgccgat gctgctgctg ggcgcctacg gcggcctgct ggcggaccgg ttcgacaagc
      541 gcaggctgct gatgctcacg cagctgggca tggtggtgcc cgcgctggcc ctcttcgccg
      601 tgaccgccac cgacgtggtg gcgccctgga tggtgttcgc gctcgtgctg gcccgcggca
      661 ccgtgaacgc gctggacaac cccgcccgcc agagcttcgt gatcgagatg gtcggccccg
      721 accgggtggt caacgccgtg tcgctgaaca gcgtgatcgt gcactgcgcg cgcatggtcg
      781 gcccggccat cgccggcgtg ctgatcgcca cggtgggcgt cgagccctgc ttcctcatca
      841 acgccgcgtc gttcatggcg atgctgatcg cgctgcgccg catggacgcc gcggcgctgg
      901 cccggcccga ccgggcgcag cggcgcccgc acgcgctgcg cgacgggctg gcctacgtgg
      961 cccgcacgcc cgcgctggcc gtgccgctgg cgatgatggt gctggtcggg acgctgggct
     1021 tcaacttcca ggtgatcctg ccgctgctgg ccaagttcac gttcgacggc ggggccggca
     1081 cgtacgccgc gctggtggtg gcgatgggtg ccggatcggt ggtcggcgcc ctgaccgccg
     1141 gcgcccgcgg ccgcgtgtcg ccggcgctgc tggtgggcgc cagcgccgcg ttcggcctgg
     1201 ccacggttct ggccgccgtg gcgcccacgc tgccgctgac ggtggccgcc ctggcgctgg
     1261 tgggcgcggc cagcgtgacc ttcgccgccg gcgtgaactc caccctgcaa ctggaggtgg
     1321 cccccgagat gcgcggccgc gtgatggcgc tgtactcggt ggtcttcctg ggctcgaccc
     1381 ccatcggcgg cccgctggcg ggctgggtgg cccaggcggc cagcccgcgc gccgcgctga
     1441 tggtgggcgc ggcggccgcg ctcatcgccg cgggtggggc cgcggtcgcg ggtgcgcgtg
     1501 gccggaaata tcgcggtacg caggggtttc atggccgcat cgaggttggc gcctccacac
     1561 acgggtagcc ccgggctagg gcaggggtca aagacgaccc cacgaggaga gcaagatgcc
     1621 gagcaccaag agcagaagca ggaccccggc ccgtcgacgg ccgggctccc gatcgcagca
     1681 cgacgcaccg tcgccccgcg aacacccgtt ccagtggctg accgaccagg cccgtagcgg
     1741 cctcaaggat cttccggcca acgcatcgtg gctgctgtcg cgggcgctcg agcccgccga
     1801 ggccgcgggg gacgcggccg ggtccgccgc ccacgacacg cgtgaccgcg cccgcaaggt
     1861 caaggcgtcg gtcaaggacg cggtcccggg tggcgactcg gtcgaggagc gcatgaagcg
     1921 cgcccgcgag gcggccgagc gagcgcagga cgccgagaac gacgcggtcg aggcggcccg
     1981 cgaggccaag gagcgctccg agcgcgcgaa ggaggtcggc gagcagggcc gccggcacgt
     2041 ggccgccgtc cagaaggaga gcgcggacaa cgcgaagggc cgtgtggccg aggcccggcg
     2101 ggtcgcggac gagaaggtgg ccgccgagca ggccgccgcc gaggcggatg ccgaggagga
     2161 ggtacgccgg gcggagcagg aggcccaaga ggaggccgag gccgcccagc aggaggccga
     2221 ggaggcacag gcgaacgcga agaagcagct ggaggaggcc aacgagcggc tggccgaggc
     2281 ccgccggctg gccgacgagg ccagcgccgc ggctcgcgag gcggccgagc aggcccagcg
     2341 tcaggcggag cagctggcgg cggacgcgga cgagcagcgc cgccaggccg acgccaaggt
     2401 cgaggccgcg gaggcggtcc gggcgcgggc cgacaccgac gcgaagacca cgacccggca
     2461 gatcaagcgc gagcaggtga acggcgacct caaggaccgc accaagcccg agctgctgga
     2521 cctggccgcg gcggtcgaga tcgagggccg gaccaccatg accaaggacc agctggtcaa
     2581 ggccatcgac aaggaggcgg cgcgatgaac ccgttccaca gaaagagccc gctcgagaag
     2641 ctcggcgact cgctgggcga cgtggtcaag cggatcccca aggcgctgga gagcgtgccc
     2701 cgcccgggca ggtcgggcgt ggccgcgctc gggggcctcg cggggctggc cgcggggagc
     2761 gcggcgctct cgtcgcggcg gcggcggagc gaggactcgg acggcgacgc atgaccgagc
     2821 ccacgctggt ccgggcccgg gtgctggcgc gggtgctggg cgtcaagctg ctgaccctcg
     2881 aggcgcaggt ggtgctcgag cccgcggcag cggcgcccga gccggcggcg ttgccgcccc
     2941 ggcgcggccc gacgggggag ccgcggccga tcggggtcgg gctggcgcag gccacgcgtg
     3001 cgatcgacga gggggaggcc agcctggccg cggcccgcaa cggcgtcagc ggcgcctgac
     3061 cctcacctga cgcgcctcga tctgacgctg gccgtacgag ccgcggtaca ccgggccggc
     3121 ggcggtgacg gtgtcgcccg ggcgcggcag cgggtcgggc cgcaggtcac gccgcacgtc
     3181 gacgaccgac agcccggggc cggtgacgtc gcccccggcc agcacgaagt gggcgtcgcc
     3241 gtcgccgtcg gggtcgaccg cctcgacgta gaccacgcgg ccggtggcgc tggtgcagtt
     3301 ggcggctccc gcggggcagc gggcgggctc cagcggcgcc ggcctcggcg cggtccgggc
     3361 ggtggtcgcg ggcgcggtcc gctcggtggt ggccacggtg cgtggcggct cgccgggctc
     3421 gtcgtcacct ccgcagcccg cgcccgccgc tgcgagcacc accacggcgg tcatcgccgc
     3481 cgcccgcctc acggccgctc gacgaaggcc acgaccagcg ggtcgccgct ggtgaacacg
     3541 ttcacccgcc gggccgcgat gcggggaccg gcgaagccgc ccgcgagccg cagccgccgc
     3601 gtggcgcgca cggtcagctc ggcctccgcg ccgcactcgg ccgtgagcgt ccagcgcccg
     3661 ctgcgggccg ccttgggcac gcgccagcgc caggtcacgt agcggtgctc ggtgagcacg
     3721 tcctcggtgg tctgggtcag gcggccgcgc ttgaaggtca ggtggcaggt gcgctcgccg
     3781 gtgaacgaca ggcgcacccg catctgggcc ggctctccgg cgcgcaggag gggggtcgcg
     3841 cggtcgatgt cgatccgggc cgcccgggcc ggggcgggca gggcgaccag gcaggcggcc
     3901 acgcagatca aggtcctggc ggcgcgcatg cccgggacgg tacgaggcca ggcggtcagt
     3961 cgaccagcga accgatggcc gcgagcgcgc gctgggcgtt cagccgcccg ttgtaggcca
     4021 ccttgccctt gagcgcgggt ggcgtgtcca ccttgtggcg gatcgcgtag cggaggtccg
     4081 agtaggtggc gcccgactcc tgcttgcgca gcatggccgc cgtgcccgcc gcgtacggcg
     4141 acgccatcga ggtgcccgac agcagcttgt agccgccgcc caggtaggtc gacaggatct
     4201 ggttgccggg cgcggccacg tccacggcgg tcgagccgta gttggagaag gaggccagcg
     4261 tgtcggtcga ggtggtggcg gccacggtca ggatgttcga gtcggtgtac gaagccgggt
     4321 actcgggctt cttgtcgatg ttgtcgccgt cgttcccggc ggccaccacc agcagcgagc
     4381 ccttgctctg ggcgtagtcg acggcgtcgt gcagcgcgga ggacttggag ctggagccga
     4441 acgagcagtt gatgaccttg gcgcccttct tcgcggcgta ctggatgccg tcgatggcgt
     4501 ccgaggtcga gcccttgccc ttcgagttca tgaacttgac cgccatcagc ttggtcgacc
     4561 agcagatgcc gctgacccct gtgccgttgt tgccgcgccc gccgatgatc cccgacacgt
     4621 gcgagccgtg gccgttgtcg tcctcgcccg agcccttgcc cttgatcacg ttgagcccgt
     4681 acgtgtcgtc cacgtagccg ttcttgtcgt cgtccttgcc gttgttgggc ttgtccgagc
     4741 tcttgtagat gttgggcgcc aggtcgggat ggtcggtgtc gatcccggtg tccagcacgg
     4801 cgaccgtcga gcagctggtg cgcgtgcccc aggcctcggg tgcgctgatg gccccggtcg
     4861 cgctgttcgc gacggcgtac tggaacgggt agtccggatc gttcggcgtg cgcgtggccg
     4921 cggtgctcag cgtgaagtcg ggctccgcgt aggtgacgcc gtcggccttg cgcaggacct
     4981 tgcggaccgc gtcgagccgc ctgccgccgc gggcgttcac cgccaccagc cgcccgccgc
     5041 ggacggcggc gaagcgcttc tcgatccgcc cgccgagctc gccgacgatc ttgccctggc
     5101 gctccttgga gacgcccttg tcgaatccga ccagcacccg ctcggccgcg ccggccggag
     5161 cgggcgcgga cagcgccgcc cccacggccg ccaggatcat caactcacgc cggtgacgca
     5221 tcggccgccg cctttctgtc gcgttcggcg aagaacgact cgccctcgat cgagatctcc
     5281 ggcagcgcct tggccagcca gcgcggcagc caccagctgg cggcgccgag cttgaccatg
     5341 accgcgggca cgagcaggca gcgcaccagc gtggcgtcta tggcgatggc caccgccagc
     5401 ccgaccccga actgcttgac ggtcggatcg ccgttgagga tgaacgagct gaacacgaac
     5461 accatgatca gcgcggccga ggtgatcacc cgcccggtcg tggccagccc gtcgatgacc
     5521 gcctggcgcg cgtcaccgga ctcgttgtag tgctccttga tctgcgtcag caggaacacc
     5581 tcgtagtcca tcgacaggcc gaacaggatc gcgaacatca gcagcggcac gaagctggcg
     5641 atgggcgtcg cgccgtccag cccgatcagc gtggcgccgt ggccctcctg gaacacgaag
     5701 gtgaccacgc cgtaggccgc ggccaccgag agcaggttgg cgatgccggc cttcagcggc
     5761 atcagcaccg accggaaggc cagcagcagc acgatgaagc tgagccccac gacgatcgcg
     5821 atcatcgacg gcagcttgtc cgagatccgg tcggccaggt cgatgtaccc ggcggtctgg
     5881 ccgcccacgt aggcctcgag gtcggtgccc ttgaccgcgg ccgggatcgt ggtgtcgcgc
     5941 agctcgcgca ccgtggtctc ggtcttgcgg tccgccggcg cggtggtggc gatggcggtg
     6001 aacaccgcgg tgtcggcctt cttgtccagc acgggcggcg tcaccgactt gatccccggc
     6061 gccttggcca cgtcgtcgga cagggtcgtc aggcgcgcgt ccgtggccgg gttctcggcc
     6121 ttcttcttct ggtcgtcgag cttctgctgc tgggacgcgg tcttctgctt ggcctggtcc
     6181 ggcggcaggc cctgggcctc ggcctgctgc tgttgctggt cgatctgctt ctgctggtcg
     6241 tccagctcgt tcaggttctt ctggtcgggc ttggccggcg accccagctg cgtggcgatc
     6301 aggagcgggc cgttctggcc caccccgaag cccagggtca ggaggtcgta ggcctggcgc
     6361 tgctgcgtgt ccttcggggc ggcggccacg tcggccaccc ccaggtgtag gttcagcacg
     6421 ggcgcggcca gcaccgccag gacggccacg gccgcgacga tcgaggtggc gggccgcttg
     6481 gctaccccgc gcgcccagcg ccgccagccg tgcggctcgt ggtcgtccgg gtgggtcttg
     6541 cccagctgca cccgcagcga gttgatccgc ggcccgagcg cgcccagcag cgccggcagc
     6601 agcgtggtgg ccgcggcaac ggcgaccgcc accgccaccg ccgccgtgta ccccagggcg
     6661 ctgaccagcg ggatcccggc gaaggccagc gacaccagcg cgatcaccac cgtgaagccc
     6721 gcgaacgcca cggcgccgcc cgcggtggcc gtggcccggc cgatcgactc ggtcatgtcc
     6781 atgccctcgc ccagctggat cttgtgccgg gtgacgatga acagggcgta gtcgatgccc
     6841 acgccgagcc cgatcatggt ggccagcgtc gcgccgacgg atggcacctc gagcgcgtgg
     6901 cccagcaggc cgatggccga cagcgagatg accagcccca ggaccgcggt cacgatgggc
     6961 agcgccatcg cggtggcggt cccgaaggcg aacagcagga tcaccaccgc ggccgtcagc
     7021 ccgaccacct cgctggactc ggtcgcgggc ttggacacgg ccttgcccac gtagccgccc
     7081 gtcgccgtgt ccaggccggc gtcgctcagc ggcttggagg cgtcgacgac cttctgggcg
     7141 tcgtcctcgg tcaggtcggc gggcccctcg ctcaggaaca ccgagatgta cgccgactgc
     7201 ccgtccttgc tcagcgccgg gttcttggag ccctggagcg ggtcggtggc cttgatcacg
     7261 cccggcacct tgcccagcgc ggtcaccgtg tcctcgatcg ccttcctggg ggccgagtcg
     7321 gtgagcttgc ccttgtcggt gtgcaccacc accgggttcg acccgtaggc ctgatccggc
     7381 agcttgctgg acagcaggtc ctgggactgg gtggagcccg tcccgggcag cgacaggttg
     7441 tcgctggtct ggctaccggc cgaccgggcc gccgcgacga tggccaccgc cagcacgagc
     7501 cagagcccga tcacgaccca gtggtgccgc gcacaagcgc gaccgaggcg atagaggaca
     7561 gccgccaccc cccgacgcta accgggcgcc ggtcgcgcgt gcaagcccgc ccgacggggg
     7621 cttaatcggc gctcttgagc ttccgcgcgc gcttgacgcg gtcggtcttg gccaggtcga
     7681 gcttgcgcag gcgcaccgag tccggcgtga cctccacgca ctcgtcctcg cgcaggaact
     7741 c
//
LOCUS       DASUPW010000570         2833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_685750, whole genome shotgun sequence.
ACCESSION   DASUPW010000570 DASUPW010000000
VERSION     DASUPW010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685750"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VFY44_12875"
     CDS             <1..250
                     /locus_tag="VFY44_12875"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX5911379.1"
                     /translation="ADSALGCAVQTTLAAGRAYTTLETKVNMIRPIHASTGRVLADAT
                     VLHSGRTTATAECRVTAEDTGKLLAHGTTTCAVFDFKG"
     gene            complement(247..726)
                     /locus_tag="VFY44_12880"
     CDS             complement(247..726)
                     /locus_tag="VFY44_12880"
                     /inference="COORDINATES: protein motif:HMM:NF020177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthrone oxygenase family protein"
                     /protein_id="HEX5911380.1"
                     /translation="MPGALTGATLAVAVGCALNGGVFFAFSSFVMAGLKRLPAPQGMA
                     AMQSLNVTAVTPLFMTVLFGTAAAALAVIVWSAVGADGTARVLAMAGGAVYLAGVVGV
                     TAAANVPLNDRLAAAGATDPSSVQLWADYLSGWTMWNHVRTVAGIVAAGLLVAAAAA"
     gene            complement(726..1571)
                     /locus_tag="VFY44_12885"
     CDS             complement(726..1571)
                     /locus_tag="VFY44_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911381.1"
                     /translation="MTSQQTPILVIGGTGKTGARVAARLAARGQGVRIGSRSADPAFD
                     WEDRSTWGPALEGTQAAYISFFPDLAVPGAPEAVAALAREALRVGTRRLVLLSGRGEA
                     EAQRAEAELAASGAEWTVVRASWFAQNFSEAQFLEPLAAGELALPADAGREPFVDADD
                     IADVAVAALTEPGHSGRVYEVTGPRLLTFGQAVAEIAEASGRELRYQPIAMADFEAGM
                     TAAGEPEEVVALLRYLFTEVHGHNVTIGDGVQRALGRPPRDFADYARDAAAAGTWDAA
                     GRSAA"
     gene            1671..2627
                     /locus_tag="VFY44_12890"
     CDS             1671..2627
                     /locus_tag="VFY44_12890"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX5911382.1"
                     /translation="MPGPVTPIPPRPDPLGEALHFLRMDGAFYCLSELTAPWGFTLEP
                     MPGHIWFHVVTAGAMWLDAGDHEPRALRPGELALVPHGQGHRLYSQPGVPTPDIHELG
                     LERVSDRYEQLTAGGGGEPTRLICGAVRFDHPVARNLVDMLPPMVRVQAGGSPQADRM
                     QGTLSLMAAEAREPRPGGEAVITRLADILVIQAVRSWIETDPAARTGWLGALQDPQIG
                     HAITLIHADPARAWSVASLADELAMSRSAFAARFTELMGEPVMQYVTRWRMHVAGDEL
                     EQGATVGQLAGRLGYASEAAFARAFKRVTGMPPGAVRRRAAA"
     gene            complement(2629..>2833)
                     /locus_tag="VFY44_12895"
     CDS             complement(2629..>2833)
                     /locus_tag="VFY44_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911383.1"
                     /translation="IVASGGFDSERIRRFEAEGAPVDAYGVGSSLLRGQNDFTADVVL
                     LDGESVAKVGREHRPNPRLERVE"
BASE COUNT          370 a         1117 c         1002 g          344 t
ORIGIN      
        1 ggcggactcg gccctgggct gcgccgtgca gaccacgctg gccgccggcc gcgcctacac
       61 cacgctcgaa accaaggtga acatgatccg gccgatccac gcctcgaccg ggcgggtgct
      121 ggcggacgcc accgtgctgc acagcggccg gaccaccgcc accgccgagt gccgcgtgac
      181 cgccgaggac acgggcaagc tgctggccca cggcaccacc acctgcgcgg tgttcgactt
      241 caagggctag gccgccgcgg cggccaccag caggcccgcc gcgacgatcc cggccaccgt
      301 ccggacgtgg ttccacatcg tccagccgga caggtagtcg gcccagagct gcaccgacga
      361 cgggtcggtg gctcctgccg ccgccagccg gtcgttcagc ggcacgttcg ccgccgcggt
      421 cacgccgacc acgccggcca ggtacaccgc gccgccggcc atggccagca cccgcgccgt
      481 gccgtcggcg cccaccgcgc tccacacgat cacggccagc gccgccgcgg ccgtgccgaa
      541 cagcacggtc atgaacagcg gggtgaccgc ggtcacgttc agcgactgca tggccgccat
      601 cccctgcggc gccggcaacc gcttcaggcc cgccatcacg aagctggaga aggcgaagaa
      661 cacgccgccg ttcagggcgc agcccaccgc gacggccagc gtcgctcccg tcagcgcgcc
      721 cggcatcagg ccgcgctccg gccggccgcg tcccacgtcc cggcggcggc cgcgtcgcgg
      781 gcgtagtcgg cgaagtcccg cggcggacgg cccagcgccc gctggacgcc gtcgccgatg
      841 gtcacgttgt gaccgtggac ctcggtgaac aggtagcgca gcagggccac gacctcctcg
      901 ggctcgcccg ccgcggtcat gcccgcctcg aagtcggcca tcgcgatcgg ctggtagcgc
      961 agctcgcgcc cgctggcctc ggcgatctcc gccaccgcct ggccgaaggt cagcagccgc
     1021 ggcccggtga cctcgtagac acggcccgag tggcccggct cggtcagcgc ggccacggcc
     1081 acgtcggcga tgtcgtcggc gtccacgaac ggctcgcgcc ctgcgtccgc cggcagcgcc
     1141 agctcgcccg ccgccagcgg ctccaggaac tgggcctcgc tgaagttctg cgcgaaccac
     1201 gacgcgcgga ccacggtcca ttcggctccg gaggcggcca gctcggcctc ggcccgctgc
     1261 gcctcggcct cgccacggcc cgagagcagg accagccggc gtgtgccgac ccgcaacgcc
     1321 tcacgcgcca gcgcggccac ggcctcgggc gcgccgggca cggccaggtc ggggaagaac
     1381 gagatgtacg cggcctgcgt gccctccagc gccgggcccc acgtggaccg gtcctcccag
     1441 tcgaaggcgg ggtcggcgga gcgcgagccg atccgcaccc cctgcccgcg cgccgccagg
     1501 cgtgccgcca cgcgggcgcc ggtcttgccg gtgccgccga tcacgagaat gggtgtctgc
     1561 tgtgatgtca tgcgtccatt ctcggaatga cgtggtggcg cgtctatgac cctcagggcc
     1621 ggattcatgc tcgatcgtcc acagccctgg atgtccggta cggtggttcc atgcccggcc
     1681 cggtgacgcc gatcccccct cgccccgacc cgctgggcga ggcgctgcac ttcctgcgca
     1741 tggacggcgc cttctactgc ctgtccgagc tgaccgcgcc gtggggcttc acgctggagc
     1801 cgatgcccgg tcacatctgg ttccacgtgg tcaccgccgg ggcgatgtgg ctggacgcgg
     1861 gcgaccacga gccccgcgcg ctgcgccccg gcgagctggc gctggtcccc cacggccagg
     1921 gccaccggct gtacagccag cccggcgtgc ccactcccga catccacgag ctcgggttgg
     1981 agcgcgtgag cgaccgctac gagcagctca ccgccggcgg gggcggcgag cccacccggc
     2041 tgatctgcgg cgccgtgcgc ttcgaccacc cggtggcgcg caacctggtg gacatgctcc
     2101 cgccgatggt ccgcgtccag gccggcggct cgccgcaggc cgaccggatg cagggcacgc
     2161 tgtcgctcat ggccgccgag gcgcgcgagc cgcgcccggg aggcgaggcg gtgatcaccc
     2221 gcctggccga catcctggtg atccaggccg tgcgctcgtg gatcgagacc gacccggccg
     2281 cccggaccgg ctggctgggc gccctgcagg acccgcagat cgggcacgcc atcacgctca
     2341 tccacgccga tcccgcgcgg gcgtggagcg tggcctcgct ggccgacgag ctggccatgt
     2401 cacggtcggc gttcgccgcc cggttcaccg agctcatggg cgagccggtc atgcagtacg
     2461 tgacccggtg gcggatgcac gtggccggcg acgagctgga gcagggggcg acggtgggcc
     2521 agctggcggg gcggctgggc tacgcgtcgg aggcggcgtt cgcgcgggcg ttcaagcgcg
     2581 tgaccggcat gccccccggc gcggtccggc gccgggccgc ggcctagctc actcgacgcg
     2641 ttccagccgc gggttgggcc ggtgctcgcg cccgaccttg gccaccgact cgccgtccag
     2701 caggaccacg tccgcggtga agtcgttctg gccccgcagc agcgacgagc ccacgccgta
     2761 ggcgtccacc ggcgcgccct cggcctcgaa ccgccggatg cgctcggaat cgaacccgcc
     2821 cgacgccacg atc
//
LOCUS       DASUPW010000571         4783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_410450, whole genome shotgun sequence.
ACCESSION   DASUPW010000571 DASUPW010000000
VERSION     DASUPW010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410450"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VFY44_12900"
     CDS             complement(<1..296)
                     /locus_tag="VFY44_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911384.1"
                     /translation="MPRRETKRGAERTPLSGDYDVLICGASFAGLAVARQLAGATRAD
                     GTPARVLMIDRYEIGERQTSACGIPTVWLDALGLMGSHRQTFHELRVSTPFGTH"
     gene            357..1841
                     /gene="rpsA"
                     /locus_tag="VFY44_12905"
     CDS             357..1841
                     /gene="rpsA"
                     /locus_tag="VFY44_12905"
                     /inference="COORDINATES: protein motif:HMM:NF005208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX5911385.1"
                     /translation="MTETATLTPTAKPVEGSDGLLLEIDGQIVPNYDATLVPFEEGDV
                     VTGNVVRIDQDEVLVDIGYKSEGVILANELSIRKSVDPADEVELGEEVDALVLTKEDQ
                     DGRLLLSKKRARFEKAWRRIEAAAESGEPVEGTVIEVVKGGLIIDLGVRGFLPASLVD
                     IRRVQNLDEFMSTKIECKVIELNRSRNNVVLSRRAVLEEERKEVRQQILDRLQPGQIV
                     EGDISNIVDFGAFVDLDGIDGLIHISELSWSHVNHPSEILSIGDTVPVKVLDIDRDRQ
                     RISLGLKQTQEDPWQRVVDTYNLGDELEGKVTKVVTFGAFVEIMDGVEGLVHISELAH
                     HHVENPREVVEPGQDVKVKILEIDSERRRLSLSVKRVAGNEPPPGSEGSGGDGGPRGG
                     GGGDDVFAGPSIAGLGDLRAAMDAQAAAAADAEEAPPLEETAPAEESPAEEAPAPEAA
                     APAEDAPAEDAPAEEEAPVEETAAPDEPEAEADAPAAEPESDES"
     gene            1867..2430
                     /gene="coaE"
                     /locus_tag="VFY44_12910"
     CDS             1867..2430
                     /gene="coaE"
                     /locus_tag="VFY44_12910"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX5911386.1"
                     /translation="MTGGMASGKSTALDLLEQLGAATISSDAVVHDLLGSDEVRDAVA
                     ARLGDEVIGADGALDRGAMAAKVFASDADREWLEQLLWPRVRERVAQWYAGLEGVPAA
                     VVEVPLLFESGGEAVYHHTIAVVADEEVRSERAGARGHEGVDERTGRQLTQDEKSQRA
                     DFTVRNDGTREELRAQLSRVLATIGED"
     gene            2547..3107
                     /locus_tag="VFY44_12915"
     CDS             2547..3107
                     /locus_tag="VFY44_12915"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX5911387.1"
                     /translation="MLVAAAVGAFAAASIAGVGPLGDELREVTLPLKHEDIIRQQAAD
                     KDLDPALIAAVIFEESRFRDQTSHAGARGLMQITPQTAAFIARDSGGTAFVQGDLATP
                     QVNISYGAYYMRYLLRRYDGDVTLAVAAYNAGETNVNKWVDRAGGRDSFDAEDDVPFK
                     ETRDYVRDVKQKRQEYRDNYKDELGL"
     gene            complement(3220..3591)
                     /locus_tag="VFY44_12920"
     CDS             complement(3220..3591)
                     /locus_tag="VFY44_12920"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX5911388.1"
                     /translation="MRWIVDAMNVIGTRPDGWWRDRDAAMARLVDRLERYSAQTGDDV
                     TVVFERKPNPPIRSTVIQIAHARKRGPDAADFEIVKIVEADANPKSIQVVTSDRWLGD
                     RVSALGATVFPSEPFRSRIDD"
     gene            complement(3596..>4783)
                     /locus_tag="VFY44_12925"
     CDS             complement(3596..>4783)
                     /locus_tag="VFY44_12925"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5911389.1"
                     /translation="ESNSPARPLYLGYVGSTGLEVGVLGAALAATYDVNLAATARAAD
                     LVEAQALQWVAQLVGFPLSEGAFTSGGMTSNMTALLVAREHALPGSRRDGFDGTKAAV
                     YCSDEAHHSVVRAAEAAGIGQQGVRKLPIDDHRRVRPADVAAAIAADRERGITPVAVV
                     ANGGTTLTGSVDPLDGLADACADAGVWLHVDGAYGLPAACTARARDLFAGLERVDSVT
                     LDAHKWLGIQKGCSVVLVRESGALERSFGHEESYIRRTGSVNLVERTLEYSRPINSLK
                     LWLALRTHGAAAFRGWIEHTLGLAAQLVRLVRADPEFELLHEPQLSTVCFRHVPEGVD
                     DLDAHNSALADAAVRDGRVFLADAVVDGTRCLRVCFVNFRTRTEDVAFALDTLRALGR
                     DLA"
BASE COUNT          722 a         1716 c         1722 g          623 t
ORIGIN      
        1 tagtgggtgc cgaacggcgt cgagacgcgg agctcgtgaa acgtctggcg atgcgagccc
       61 atcagcccga gcgcgtcgag ccagaccgtg gggatgccac acgccgaggt ctggcgctcg
      121 ccgatctcgt accggtcgat catcagcacg cgcgcgggcg tgccgtccgc gcgggtggcg
      181 ccggccagct ggcgggccac cgccaggccg gcgaagctgg cgccgcagat caggacgtcg
      241 tagtcccccg agaggggggt gcgctctgcg ccgcgcttgg tctctcgacg tggcatcgac
      301 ggagcagggt accgggactg ctgtttaccg cgaggcgtgt gtaccctgca gggttcgtga
      361 ccgagaccgc caccctaacc ccaaccgcca agcccgtaga gggctccgac ggcctcctgc
      421 tggagatcga cggccagatt gtccccaact acgacgccac gctcgtcccc ttcgaagaag
      481 gtgatgtggt taccggcaac gtcgtccgga tcgaccagga cgaggtcctt gtcgacatcg
      541 gctacaagag cgagggggtc atcctcgcca acgagctgag catccggaag tccgtggacc
      601 ccgcggacga ggtggagctc ggcgaagagg tcgatgcgct cgtcctcacc aaggaggacc
      661 aggacggccg cctgctgctg tccaagaagc gcgcgcgctt cgagaaggcg tggcgccgca
      721 tcgaggccgc cgccgagtcc ggcgagccgg tcgagggcac ggtcatcgag gtcgtcaagg
      781 gcggcctgat catcgacctg ggcgtgcgtg gcttcctgcc cgcatcgctc gtggacatcc
      841 gccgggtgca gaacctggac gagttcatgt ccacgaagat cgagtgcaag gtcatcgagc
      901 tcaaccgctc gcgcaacaac gttgtgctct cgcgccgcgc cgttctggag gaggagcgca
      961 aggaggtccg ccagcagatc ctggaccgcc tgcagcccgg ccagatcgtg gagggcgaca
     1021 tctcgaacat cgtcgacttc ggcgcgttcg tggacctgga cggcatcgac ggcctgatcc
     1081 acatctcaga gctttcctgg tcgcacgtga accacccgtc cgagatcctc tcgatcggcg
     1141 acaccgtgcc ggtcaaggtc ctggacatcg accgcgaccg ccagcgcatc tccctgggcc
     1201 tcaagcagac ccaggaggac ccatggcagc gcgtggtgga cacctacaac ctgggcgacg
     1261 agctcgaggg caaggtcacc aaggtcgtca ccttcggcgc gttcgtcgag atcatggacg
     1321 gcgtcgaggg cctggtgcac atctccgagc tggcccacca ccacgtggag aacccgcgtg
     1381 aggtcgtgga gcccggccag gacgtcaagg tcaagatcct ggagatcgac tccgagcgcc
     1441 ggcgcctgtc gctgagcgtc aagcgcgtgg ccggcaacga gccgcccccc ggcagcgagg
     1501 gctccggcgg cgacggcggc ccccgcggtg gcggtggcgg cgatgacgtg ttcgccggcc
     1561 ccagcatcgc cggcctgggc gacctgcggg ccgcgatgga cgcgcaggcg gcagcggccg
     1621 ccgacgcgga ggaggctccc ccgctggagg agaccgcccc ggccgaggag tcacccgccg
     1681 aggaggcgcc cgctcccgag gccgccgcgc ccgccgagga cgcgcccgcc gaggacgcgc
     1741 ccgcggagga ggaggccccg gtcgaggaga ccgcggcccc cgacgagccg gaggccgagg
     1801 ccgacgcgcc cgccgccgag cccgagtccg acgagtcctg acgccacccc cataccgttc
     1861 gtagggctga ccgggggcat ggcctccggg aagtccacgg cgctcgacct gctggagcag
     1921 ctgggcgccg ccaccatctc ctcggacgcg gtcgtccacg acctgctggg cagcgacgag
     1981 gtccgtgacg ccgtcgccgc gcgcctgggc gacgaggtca tcggcgccga cggcgccctg
     2041 gaccgcggcg ccatggcggc gaaggtgttc gcctccgacg ccgaccgtga gtggctcgag
     2101 cagctcctgt ggccgcgcgt gcgtgagcgc gtggcccagt ggtacgccgg cctggagggc
     2161 gtccccgcgg cggtggtcga ggtcccgctg ctgttcgagt ccggcggcga ggcggtctac
     2221 caccacacca tcgccgtcgt ggccgacgag gaggtccggt ccgagcgggc gggcgcccgc
     2281 ggccacgagg gcgtcgacga gcggacgggc cgtcagctca cgcaggacga gaagtcgcag
     2341 cgagcggact tcaccgtgcg caacgatggc accagagagg agttgagggc acagttgtcc
     2401 cgtgtccttg ccaccatcgg ggaggactga tcttcgtggc acaggcagcg cgcacacgca
     2461 ccaggtcgcg cacccgctcg gcgccatcgc gccggcgcgc ttcccgccgc cggaccgcga
     2521 cccaccgccg gcgacggatc atcgccgtgc tggtggccgc cgccgtgggc gcgttcgccg
     2581 cggccagcat cgcgggggtc gggccgctgg gcgacgagct gcgagaggtc accctgcctc
     2641 tcaagcacga ggacatcatc cgccagcagg ccgccgacaa ggacctggac ccggcgctga
     2701 tcgccgccgt gatcttcgag gagtcccgct tccgcgacca gacctcccac gccggcgcgc
     2761 ggggcctgat gcagatcacg ccccagaccg cggccttcat cgcccgcgac tcgggcggca
     2821 ccgcgttcgt ccagggggac ctggcgacgc cgcaggtgaa catctcctac ggcgcctact
     2881 acatgcgcta cctgctgcgg cgctacgacg gcgacgtcac cctggccgtg gccgcctaca
     2941 acgccggcga gaccaacgtg aacaagtggg tcgaccgggc cggcggccgc gactccttcg
     3001 acgccgagga cgacgtgccg ttcaaggaga cccgcgacta cgtccgcgac gtcaagcaga
     3061 agcgccagga gtaccgggac aactacaagg acgagctggg gctgtaggcc agcgacgggc
     3121 acgtcgccac ctccgaatca ccggttcctg cgtttgggac gtcccagcga cccaaacgta
     3181 tgaaccgttt ggcccgtgcc aagtgccacg ggggagcccc tagtcgtcga tccggctgcg
     3241 gaacggctcc gaggggaaca ccgtcgcgcc cagggcgctg acgcggtcgc ccagccagcg
     3301 gtcggaggtc acgacctgga tcgacttcgg gttggcgtcg gcctccacga tcttcacgat
     3361 ctcgaagtcg gccgcgtccg gcccgcgctt gcgcgcgtgg gcgatctgga tcaccgtcga
     3421 gcggatcggc gggttcggct tgcgctcgaa caccacggtc acgtcgtcgc cggtctgggc
     3481 gctgtagcgc tccagccggt ccaccagccg ggccatcgcg gcgtcgcggt cgcgccacca
     3541 gccgtcgggg cgcgtgccga tcacgttcat ggcgtcgacg atccagcgca tcggctcagg
     3601 ccaggtcgcg gcccagggcg cggagggtgt ccagggcgaa ggccacgtcc tcggtccggg
     3661 tgcggaagtt cacgaagcac acgcgcaggc agcgggtccc gtcgaccacg gcgtcggcca
     3721 ggaacacgcg gccgtcccgg accgcggcat cggccagggc ggagttgtgg gcgtccaggt
     3781 cgtcgacccc ttcggggacg tggcggaagc agaccgtgga cagctggggc tcgtgcagca
     3841 gctcgaactc ggggtcggcg cggaccaggc ggaccagttg ggccgccagg cccagggtgt
     3901 gctcgatcca gccccggaac gcggccgcgc cgtgcgtgcg cagcgccagc cacagcttca
     3961 gcgagttgat cgggcgcgag tactccagcg tgcgctccac caggttcacc gagccggtgc
     4021 ggcggatgta ggactcctcg tggccgaagc tgcgctccag cgcgcccgac tcacgcacca
     4081 gcaccacgct gcaccccttc tggatgccca gccacttgtg ggcgtccagc gtgaccgagt
     4141 cgacccgctc cagcccggcg aacaggtcgc gtgcgcgggc cgtgcaggcg gcgggcaggc
     4201 cgtaggcccc gtccacgtgc agccagacgc cggcgtcggc gcacgcgtcg gccaggccgt
     4261 ccagcgggtc gaccgacccg gtcagcgtgg tgccgccgtt ggccaccacg gccaccgggg
     4321 tgatccctcg ttcgcggtcg gcggcgattg ccgcggccac gtcggcgggg cggacgcgcc
     4381 ggtggtcgtc gatcggcagc ttgcgcacgc cctgctggcc gatcccggcg gcctccgccg
     4441 cccggaccac cgagtggtgg gcctcgtccg agcagtacac ggccgccttc gtgccgtcga
     4501 agccgtcccg ccgtgagccg ggcagggcgt gctcgcgggc cacgagcagc gcggtcatgt
     4561 tcgacgtcat gccgccgctg gtgaacgcgc cctcggacag cgggaagccg accagctggg
     4621 ccacccactg cagcgcctgc gcctccacca ggtcggcggc gcgcgcggtg gcggccaggt
     4681 tcacgtcgta ggtcgcggcc agggcggcgc ccagcacgcc gacctccagc ccggtggagc
     4741 ccacgtagcc caggtacagc ggccgcgcgg gcgagttgct ctc
//
LOCUS       DASUPW010000572         4199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1655911, whole genome shotgun sequence.
ACCESSION   DASUPW010000572 DASUPW010000000
VERSION     DASUPW010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655911"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VFY44_12930"
     CDS             complement(<1..466)
                     /locus_tag="VFY44_12930"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX5911390.1"
                     /translation="MGLPAWGFATVAGRVMGTRPPNLFLTLARNRALFWGWLLFAGRL
                     MPGGRLRRRESELVILRVAHLRECRYEWEHHMRLGRRAGVGRDDLERVQAGPEAEGWP
                     PRERALLAATDELHASSDLGEEGWSALRAHLDEREAIELCMLVGHYEMLATVI"
     gene            complement(502..1359)
                     /locus_tag="VFY44_12935"
     CDS             complement(502..1359)
                     /locus_tag="VFY44_12935"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5911391.1"
                     /translation="MALIKTPSLDGRRVLITGAARGIGAELAKRLHQRGARVAVAGLE
                     PELLEQVSAASGGGPWRECDVTDRQQVEDAVDAAVEGLGGLDVVVANAGVAAQLPLVG
                     GNPRIMEQTVAVNLMGVYYTLRAAGPHISHENGYALPVSSLAAAAHAPMLGAYSASKA
                     GVEALGNTLRAELLQTGAKVGVAYFAELDTDMTSRGFSTEAAGKMKFVARQRVAPLKA
                     GIDALERGIARRSRLVYAPRWVGPVLPIRMGAQLVVDRVGQMGLRKALETARAEDAPL
                     TTVQPGEPR"
     gene            1416..2753
                     /locus_tag="VFY44_12940"
     CDS             1416..2753
                     /locus_tag="VFY44_12940"
                     /inference="COORDINATES: protein motif:HMM:NF013836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEX5911392.1"
                     /translation="MALEASIEKMEAEGVHPAAVAAFTHAYEALAGGDTGILPEADIE
                     PVNDLPRADELPEGDPEDLGKAVVIKLNGGLGTSMGMTKAKSLVEVKDGLTFLDVIAR
                     QAVTAGVPLLLMDSFRTRDDSLAVLERYDELEGDLPLDFVQNKEPKLDAETLDPVSWE
                     ADPDLEWCPPGHGDIYPALVASGLLDQLLERGYETAFVSNADNLGATLDPRILSWFRS
                     EGLPFLMEVSRRGENDRKGGHLARRRADGQLVLRESAQTADEDEDAFQDISRHQFFNT
                     NSLWIDLHALAGKLEQEGGALPLPLIVNRKTVDPADPGSPDVIQLETAMGAAVGVFEG
                     AGGIEVARDRFAPVKTTNDLLVLRSDCYEITDDWRVVLAGGRDAQPGVDLDPEHYKLL
                     AGFEDRFPDGPPSLVGAERLVVRGDVTFGADVTVTGAVELDGPARIPDGAQLP"
     gene            2773..>4199
                     /locus_tag="VFY44_12945"
     CDS             2773..>4199
                     /locus_tag="VFY44_12945"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5911393.1"
                     /translation="MTRGLIALAVAAGLLAAPAGADAALRFKGCDSSPFRCAKVTVPL
                     DRTGQVRGKVTLRVLRAAATGRRRGVVVLLAGGPGQSAVTAYVDGGLGPLAPIRRTHD
                     VVVVDQRGSGGSGLLRCPELERANILRAARPAARCAAKLGPRRAFYTSRDTADDLDTV
                     RRALGVSKLSLYGVSYGTRTAAAYALRHPTRVRRLGLDSVTAVDGADALSVDSFRAVP
                     RVLRTLCAGRRCDRFTRDPVADVAGLVQRIGAGGPLRGTLPDRRGRPRPGTLSRYDVF
                     STLVSGDFEASLRTAYPAAVKAALRGDPAPLLRLKRRSIALESGGFEPEELSTALYAA
                     TTCEEARLPWPRGTPFAQRRPRAAAAAAALAPALIAPFDGATVLGSDVIDLCSQWPES
                     PRDPTPGPGPLPDVPALLVEGADDLRTPLETARSVAAGLSRAKVVSVAGVGHSPGSAG
                     LSRCAERLAARFLAGGRVGAACRDAR"
BASE COUNT          517 a         1599 c         1550 g          533 t
ORIGIN      
        1 cgatgaccgt agcgagcatc tcgtagtggc ccacgagcat gcacagctcg atcgcctcgc
       61 gctcgtccag gtgggcgcgc agggcggacc agccctcctc gcccaggtcg gaggaggcgt
      121 gcagctcgtc ggtcgccgcc agcagggcgc gctcgcgtgg gggccagccc tcggcctcgg
      181 gaccggcctg cacccgctcg aggtcgtccc gccccacgcc ggcgcgccgg cccaggcgca
      241 tgtggtgctc ccactcgtac cggcactcgc gaaggtgcgc gacgcgcagg atcaccagct
      301 cgctctcgcg ccggcgcagc ctgccccccg gcatcaggcg cccggcgaac agcagccagc
      361 cccagaacag cgcccggttg cgggccagcg tcaggaacag gttgggcggc cgcgtgccca
      421 tcacgcggcc cgccacggtg gcgaaccccc acgcgggcag gcccacctgc cggcgcgtgc
      481 cgggccggat gcggggctcg ctcagcgcgg ctctcccggc tgcacggtgg tcagcggcgc
      541 gtcctcggcg cgggcggtct cgagcgcctt gcgcaggccc atctgcccga cgcggtccac
      601 caccagctgc gcgcccatcc ggatcggcag caccggcccg acccagcgcg gggcgtacac
      661 cagccgcgag cgccgggcga tgccgcgctc cagcgcgtcg atgcccgcct tcagcggcgc
      721 cacgcgctgg cgggccacga acttcatctt gccggcggcc tccgtgctga agccccgtga
      781 ggtcatgtcg gtgtccagct cggcgaagta ggccacgccc accttggcgc cggtctggag
      841 cagctcggcg cggagggtgt tgcccagggc ctcgacgccc gccttggacg cgctgtaggc
      901 accgagcatc ggggcgtgcg cggccgccgc gagcgaggac accggaaggg cgtacccgtt
      961 ctcgtggctg atgtgcgggc cggccgcgcg cagcgtgtag tagacgccca tcaggttcac
     1021 ggccacggtc tgctccatga tcctggggtt gccgcccacc agcggcagct gcgccgccac
     1081 gccggcgttg gccaccacca cgtcgagtcc gcccaggccc tcgaccgcgg cgtccaccgc
     1141 gtcctcgacc tgctggcggt cggtcacgtc gcattcgcgc cacgggccgc cgccggacgc
     1201 ggcggacacc tgctcgagca gctcgggctc caggccggcc acggccaccc gcgcgccgcg
     1261 ctggtgcagc cgcttggcca gctcggcgcc gatgccgcgg gcggctcccg tgatgagcac
     1321 ccgccggccg tccagtgaag gggtcttgat gagggccatg cgcacagtaa acatgaacag
     1381 cgctgttcgt gagtcggtgt gcgtacgatg ccgccgtggc cctggaggca tcgatcgaga
     1441 agatggaggc cgagggggtg caccccgcgg ccgtggccgc cttcacccac gcctacgagg
     1501 cccttgccgg cggcgacacc gggatcctgc ccgaggccga catcgagccg gtcaacgacc
     1561 tgccgcgggc ggacgagctg cccgaggggg accccgagga cctgggcaag gccgtggtga
     1621 tcaagctgaa cggcggcctg ggcaccagca tgggcatgac caaggccaag tcgctggtcg
     1681 aggtcaagga cgggctgacc ttcctggacg tgatcgcgcg ccaggccgtg accgccgggg
     1741 tgccgctgct gctgatggac agcttccgca cccgcgacga ctccctggcc gtgctcgagc
     1801 gctacgacga gctggagggc gacctcccgc tggacttcgt gcagaacaag gagcccaagc
     1861 tcgacgcgga gacgctggac ccggtcagct gggaggcgga ccccgacctg gagtggtgcc
     1921 cgcccgggca cggcgacatc tacccggcac tggtggcctc cgggctgctg gaccagctgc
     1981 tcgagcgcgg ctacgagacg gcgttcgtct ccaacgccga caacctcggc gccaccctgg
     2041 acccgcgcat cctgagctgg ttccggtccg agggcctgcc gttcctcatg gaggtctccc
     2101 gccgcgggga gaacgaccgc aagggcggtc acctggcgcg ccgccgggcc gacgggcagc
     2161 tggtgctgcg cgagtccgcc cagaccgccg acgaggacga ggacgccttc caggacatca
     2221 gccggcacca gttcttcaac accaactcgc tgtggatcga cctccatgcc ctggccggca
     2281 agctggagca ggagggcggc gcgctgccgc tgccgctgat cgtgaaccgc aagacggtcg
     2341 acccggccga tcccggctcg ccggacgtga tccagctgga gacggcgatg ggcgccgccg
     2401 tcggcgtgtt cgagggagcg ggcggcatcg aggtcgcgcg cgaccggttc gcgcccgtga
     2461 agaccaccaa cgacctgctg gtgctgcgct cggactgcta cgagatcacc gatgactggc
     2521 gcgtggtgct ggccggtggc cgcgacgccc agcccggggt ggacctggac cccgagcact
     2581 acaagctgct ggccggcttc gaggaccgct tccccgacgg cccgccctcg ctggtcggcg
     2641 ccgaacggct ggtcgtgcgc ggcgacgtga ccttcggcgc ggacgtcacc gtgaccggcg
     2701 ccgtcgagct ggacgggccg gcccggatcc ctgacggcgc gcaactgcct tgaccacgcg
     2761 atgatggctc ccgtgacgag ggggctgatc gcgctggccg tggccgccgg gctgctggcg
     2821 gcgccggcgg gcgccgacgc ggcgctgcgc ttcaaggggt gtgactccag ccccttccgc
     2881 tgcgcgaagg tgaccgtgcc gctggaccgc accggccagg tcaggggcaa ggtcacgctg
     2941 cgcgtgctgc gcgccgctgc caccggccgc cgccggggcg tggtggtgct gctggcgggc
     3001 gggccggggc agtcggcggt gacggcctac gtcgacggcg gcctgggccc gctggcgccg
     3061 atccggcgca cgcacgacgt cgtcgtggtc gaccagcgcg gcagcggcgg ctccgggctc
     3121 cttcgctgtc ccgagctcga gcgggccaac atcctgcgcg ccgcccgacc ggcggctcgc
     3181 tgtgcggcga agctggggcc caggcgcgcc ttctacacca gccgtgacac ggcggacgac
     3241 ctggacaccg tccggcgcgc gctcggcgtg tccaagctgt cgctctacgg ggtgtcctac
     3301 ggcacgcgca cggccgccgc gtacgccctg cgccatccca cgcgggtccg gcggctgggc
     3361 ctggactcgg taaccgccgt cgacggcgcc gatgcgctgt cggtggactc cttccgcgcc
     3421 gtgccgcggg tgctgcgcac cctgtgcgcc ggccggcgct gcgaccgctt cacccgcgac
     3481 cccgtggccg acgtggccgg cctggtgcag cgcatcggcg ccggcgggcc gctcaggggc
     3541 acgctgcccg accgccgcgg ccgcccgcgg ccgggcacgc tcagccgcta cgacgtgttc
     3601 agcacgctgg tgtcgggcga cttcgaggcc tcgctgcgca ccgcctaccc ggcggcggtc
     3661 aaggccgcgc tgcgtggcga cccggcgccg ctgctgcgcc tgaagcggcg ctcgatcgcg
     3721 ctggagagcg gtggcttcga gcccgaggag ctgagcaccg cgctctatgc agcgaccacc
     3781 tgcgaggagg cacggctgcc ctggccgcgg ggcacgccgt tcgcccagcg ccgcccgcgc
     3841 gccgccgcag ccgcggcggc gctggcgccg gcgctgatcg cgcccttcga cggcgccacc
     3901 gtcctgggct ccgacgtgat cgacctgtgc tcgcagtggc ccgagtcgcc gcgcgacccg
     3961 acgcccggcc ccgggccgct gcccgacgtg cccgcgctgc tggtggaggg cgccgacgac
     4021 ctgcgcaccc cgctggagac ggcccgctcg gtcgcagccg gcctgtcgcg cgcgaaggtg
     4081 gtcagcgtcg ccggcgtcgg ccactccccg gggtcggccg ggctcagccg ctgcgccgag
     4141 cgcctggccg cccgcttcct ggccggtggg cgcgtcggcg cggcctgccg cgacgcacg
//
LOCUS       DASUPW010000573         2281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_410765, whole genome shotgun sequence.
ACCESSION   DASUPW010000573 DASUPW010000000
VERSION     DASUPW010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410765"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VFY44_12950"
     CDS             complement(<1..413)
                     /locus_tag="VFY44_12950"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5911394.1"
                     /translation="MAEKKPIEGALKAQVYKDPRPAEHFDRFHARTRAKRPNWMYEAV
                     RLVLTPYLLIFFRARAIDPDKVPADGPAIVAPNHFSFLDHFFVAVYLRRKVHFMAKSQ
                     LFKAPLQTVYHNGGVFPVRRGHRDEEAFATAHAVLA"
     gene            465..677
                     /locus_tag="VFY44_12955"
     CDS             465..677
                     /locus_tag="VFY44_12955"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEX5911395.1"
                     /translation="MPNVEAHITGTIWKVECEVGQEIADGDTVAVIESMKMEMPVESE
                     EEGTVAEIRCEEGQSVTEGDVLVVLK"
     gene            674..1468
                     /locus_tag="VFY44_12960"
     CDS             674..1468
                     /locus_tag="VFY44_12960"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5911396.1"
                     /translation="MTELASGKLVLDEPAEGVARLRIANPQRRGALDHEILDTLAETV
                     RSLDAPCLVLTGTGKVFSAGYDLGNLEGRSFEEAAEKLVAHPFHSAIEALDAYPYPVV
                     AALNGHAIGGGLELALTCDIRLAAREAKLGMPPAKLGLIYSHTGLRRFVEACGAPNTA
                     ELFYTAQNVDAERGLQMGLVGHVVDAAELDERAVAMAAGIASGAPLSLAGNKRILRVL
                     RAQASELPPDVEQELIALRESCFASEDFAEGVRAFGEKRAPRWQGR"
     gene            1503..>2281
                     /locus_tag="VFY44_12965"
     CDS             1503..>2281
                     /locus_tag="VFY44_12965"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HEX5911397.1"
                     /translation="MEDAIAQIEDQGLVSRIRLISGPQGPRVLLDGRPVLLLCSDNHL
                     GLADHPRVREAAADAAMRYGAGAGSARPGSGTMRIHRRLEERLADFHGHESALLMGSR
                     YLALLGVIPALARAGEVVLVDAGAHQAAADGCRLADAETLTYRNGDIEHLEWGLLQAQ
                     GAGSLIVTDGLSAGGDLAPLEAIVELARRHDARLLVDESHAVGAIGADGRGAAAAAGV
                     SAEVDVLVGSLATALGSYGGYVTCDRTLARYLAGAARTFTHS"
BASE COUNT          304 a          811 c          855 g          311 t
ORIGIN      
        1 gccaggacgg cgtgggcggt ggcgaacgcc tcctcgtcgc ggtgcccgcg acgcaccggg
       61 aacacgccgc cgttgtggta gacggtctgc agcggcgcct tgaagagctg ggacttggcc
      121 atgaagtgga ccttgcggcg caggtacacg gcgacgaaga agtggtccag gaacgagaag
      181 tggttggggg cgacgatggc cgggccgtcg gcggggacct tgtcggggtc gatcgcccgg
      241 gcgcggaaga agatcagcag gtagggcgtg agcaccaggc gcaccgcctc gtacatccag
      301 ttcggccgct tcgcccgcgt gcgggcatgg aagcggtcga agtgctcggc cgggcgcggg
      361 tccttgtaga cctgcgcctt gagcgccccc tcgatcggct tcttctcggc catccctacc
      421 tgcctacccg atcccgccgc ctccgttacg ggtaccttgc cttcgtgccc aacgtcgagg
      481 cccacatcac cgggacgatc tggaaggtcg agtgcgaggt cggccaggag atcgccgacg
      541 gcgacacggt ggccgtcatc gagtcgatga agatggagat gccggtggag tccgaggagg
      601 agggcaccgt cgccgagatc cgctgcgagg agggccagtc ggtgaccgag ggcgacgtgc
      661 tggtggtcct caagtgaccg agctggcgtc cggaaagctc gtgctggacg agcccgccga
      721 gggcgtggcc cgcctgcgca tcgccaaccc ccagcgccgc ggcgccctgg accacgagat
      781 cctggacacg ctggccgaga cggtgcgctc gctggacgcg ccctgcctgg tgctgaccgg
      841 caccggcaag gtgttctccg ccggctacga cctgggcaac ctggagggcc ggagcttcga
      901 ggaggccgcc gagaagctgg tggcccatcc gttccactcc gcgatcgagg cgctggacgc
      961 ctacccctac cccgtcgtgg ccgcgctgaa cgggcacgcc atcggcggcg gcctggagct
     1021 ggcgttgacc tgcgacatcc gcctggccgc gcgcgaggcc aagctgggga tgccgcccgc
     1081 caagctcggc ctgatctact cgcacaccgg gctgcgccgg ttcgtcgagg cctgcggggc
     1141 ccccaacacg gccgagctgt tctacaccgc ccagaacgtc gacgccgagc gcgggctcca
     1201 gatggggctg gtcggccacg tggtcgacgc cgccgagctg gacgagcggg cggtggccat
     1261 ggcggccggc atcgcgtccg gggcgccgct gtcgctggcc ggcaacaagc gcatcctgcg
     1321 cgtgctccgc gcccaggcct cggagctgcc ccccgacgtc gagcaggagc tgatcgccct
     1381 gcgcgagtcg tgcttcgcca gcgaggactt cgccgagggc gtgcgcgcgt tcggcgagaa
     1441 gcgcgccccc cgctggcagg gccggtagtc cgtccggagc cccgcctagc ttctgccccg
     1501 aggtggaaga tgcgatcgct cagatcgagg accaaggact ggtcagccgc atccgactga
     1561 tctccggtcc ccaggggccg cgcgtcctgc tcgacggccg cccggtgctg ctgttgtgct
     1621 cggacaacca cctggggctg gccgaccacc cccgcgtccg tgaggccgcc gccgacgccg
     1681 ccatgcgcta cggcgcgggc gccgggtccg cccggcccgg gtcggggacc atgcgcatcc
     1741 accgccggct cgaggagcgc ctggccgact tccacggcca cgagagcgcg ctgctcatgg
     1801 gctcgcgcta cctggcgctg ttgggcgtga tccccgcgct ggcacgcgcg ggcgaggtcg
     1861 tgctggtgga cgccggcgcg caccaggcgg ccgccgacgg ctgccggctg gccgacgccg
     1921 agaccctgac ctaccgcaat ggcgacatcg agcacctgga gtggggcctg ctgcaggcgc
     1981 agggcgcggg cagcctgatc gtgaccgacg gcctgtccgc cggcggcgac ctggcgccgc
     2041 tggaggccat cgtggagctg gcccggcgcc acgacgcgcg cctgctggtg gacgagtcgc
     2101 acgcggtcgg cgccatcggc gcggacggcc gtggcgcggc cgccgccgcc ggcgtgtcgg
     2161 cggaggtgga cgtgctggtg gggtcgctgg ccaccgccct gggctcctac ggcggctacg
     2221 tgacgtgcga ccgcacgctg gcgcgctacc tggccggggc cgcgcgcacg ttcacccact
     2281 c
//
LOCUS       DASUPW010000574         4517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1102724, whole genome shotgun sequence.
ACCESSION   DASUPW010000574 DASUPW010000000
VERSION     DASUPW010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102724"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1188)
                     /locus_tag="VFY44_12970"
     CDS             complement(46..1188)
                     /locus_tag="VFY44_12970"
                     /inference="COORDINATES: protein motif:HMM:NF022732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HEX5911398.1"
                     /translation="MPRPALFLLPVTAFACLAAAGPAAAKTSWLCKPGLKGNACTPSL
                     TTTLYSSSNKPLRTATPKADRKKGFDCFYVYPTVSDQKTLNATRAKDAAQREIARFQA
                     ARFGQHCRVYAPVYRQLTLKGIMGAQAGQAAGARKAYSDVEAAWKDYLKRYNDGRGVV
                     LVGHSQGAGMLSELVRRQFDRKPAARKRLIAAYLLGGNVTVKKGSDRGGSFRNVRACR
                     AATQTGCVVAFSTFNQTPPANSIFGRTGGRFEETFGQGSGADLEVLCTNPAALGGGSA
                     NLTTVLPSRPFSEKTTIGIGNKLVGITVPTPRTTYVEYRGTFSARCSSAGGARTLQVT
                     SRGGAPVPKAVPDATWGLHLLDGNIALGDLAALSAGQARAWARKNR"
     gene            complement(1198..1893)
                     /locus_tag="VFY44_12975"
     CDS             complement(1198..1893)
                     /locus_tag="VFY44_12975"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEX5911399.1"
                     /translation="MSVAGTVTQLHRWPVKSMGGEPVDTLAVDGRGAAGDRAHAVFDV
                     HKGAPRRLTARQAPRLLAWRASYDGDLADPALRAPDGTEWAWSDPGLPAALSADLGRD
                     VTLHSDPALQQDLPDSLLVTVQSTLDAVAAELGAELDLRRFRTNIHVELDADAFAEEG
                     WEGRTLRVGSAELALLHPCERCAIPTRHPDTQEKWAPLLRHLFDRHTGRFGINARARG
                     SGAIAIGDRVTLD"
     gene            1917..2522
                     /gene="def"
                     /locus_tag="VFY44_12980"
     CDS             1917..2522
                     /gene="def"
                     /locus_tag="VFY44_12980"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX5911400.1"
                     /translation="MAEEPVEVAEQPHEEESEAPRLDPEVEARRSAAMSFIKRLGDPV
                     LKSRATPVDHFDDALRGQVARMAGLMDDALGIGLAAPQLGVSQRLLVYRVGPDAPLIA
                     LANPEIEWHSDDQDVLQEGCLSLPGVQMDIERPLYVRVRARDEHGDERVVEASGLEAR
                     VIQHEMDHLDGVLILDRGSREQRKEAMRAIREAEERRERAA"
     gene            2634..2828
                     /locus_tag="VFY44_12985"
     CDS             2634..2828
                     /locus_tag="VFY44_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911401.1"
                     /translation="MSVVRKECKQCGVHTFRSQCGNCGSTELRAVGAEPETPARFVRR
                     DRNALSGIVAARGLRSGGVL"
     gene            2818..3129
                     /locus_tag="VFY44_12990"
     CDS             2818..3129
                     /locus_tag="VFY44_12990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5911402.1"
                     /translation="MSSEPTVVHNPDETRFELILDGERIGLVDYRPAGPSVIVAHTEV
                     DPGHEGEGLGGILVQAALEGIRDMGKTVIPTCPFTAAYIKRHPEWAEHVAPAMRAQFE
                     R"
     gene            3181..3600
                     /locus_tag="VFY44_12995"
     CDS             3181..3600
                     /locus_tag="VFY44_12995"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX5911403.1"
                     /translation="MTDTPRNGTELMEQFVPASPFAALVGLEVAELGDGTARLALPYR
                     PELATMGQTVHGGAIATALDTAAMAAAWSGAELPERLAGATVSMAVEYVSAANGQDLE
                     VTATLLRRGRSLCSVEAEARSPDGVLVAKALVTYKLG"
     gene            complement(3605..>4517)
                     /locus_tag="VFY44_13000"
     CDS             complement(3605..>4517)
                     /locus_tag="VFY44_13000"
                     /inference="COORDINATES: protein motif:HMM:NF013442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911404.1"
                     /translation="GTVLIETLPAAFQMDEILYELRDHSAGLNAGRWDYIFSAIKRFK
                     QRPEFVTPDRVDVTMTVPFMHAYSELLVKTCHRRGAHAMGGMSAVIPSRTDEEAAQKA
                     LDAVASDKRREAGAGFDGSWVAHPDSVEPAMGEFEKVLGDRDNQVDKQREDVDVAAAD
                     LLAIDKTEGQITEKGLRLNVNVGIQYISSWLRGNGAAAIYGLMEDAATAEISRGQIWQ
                     WIRHEATLDDGRTVTPELVRELAGEELETIRKEIGDDEWFEREGRPEESRALFEAVAL
                     SGNDTFVEFLTLPAYDKLEEIETAAKG"
BASE COUNT          590 a         1600 c         1676 g          651 t
ORIGIN      
        1 gggcggggct ggcggtggtc caggtgccgg ccggccgcgc ctagctcagc ggttcttcct
       61 ggcccaggcc ctggcctggc cggccgagag cgcggccagg tcgcccagcg cgatgtttcc
      121 gtccagcagg tgcaggcccc aggtggcgtc gggcacggcc ttgggcaccg gggcgccccc
      181 gcgggaggtc acctgcaggg tgcgcgcgcc gccggccgag gaacaccggg ccgagaacgt
      241 gccccggtac tcgacgtagg tcgtgcgcgg tgtgggcacg gtgatgccca ccagcttgtt
      301 gccgatcccg atcgtggtct tctcggagaa gggccggctg ggcaggaccg tggtcaggtt
      361 ggcgctgcca cccccgagcg cggcggggtt ggtgcaaagc acctccaggt cggcgccgct
      421 gccctggccg aaggtctcct cgaaccggcc ccccgtgcgc ccgaagatcg agttggccgg
      481 cggggtctgg ttgaaggtcg agaacgccac cacgcacccg gtctgggtcg cggcgcggca
      541 cgcgcggacg ttgcggaacg agccgccgcg gtcgctgccc ttcttcacgg tcacgttgcc
      601 gcccagcagg taggcggcga tcaggcgctt gcgcgcggcc ggcttgcggt cgaactgacg
      661 gcgcaccagc tcgctgagca tgcccgcgcc ctgggagtgg cccaccagga ccacgccgcg
      721 gccgtcgttg tagcgcttga ggtagtcctt ccacgcggcc tcgacgtcgg agtacgcctt
      781 gcgcgcgccc gcggcctggc cggcctgggc gcccatgatc cccttgaggg tcagctggcg
      841 gtagaccggc gcgtacacgc ggcagtgctg gccgaagcgg gcggcctgga agcgggcgat
      901 ctcgcgctgc gcggcgtcct tggccctggt cgcgttcagc gtcttctggt cgctgaccgt
      961 ggggtagacg tagaagcagt cgaagccctt cttcctgtcc gccttgggcg tggcggtgcg
     1021 cagcggcttg ttggacgacg agtacagcgt ggtggtcagg ctcggcgtgc acgcgttgcc
     1081 ctttagcccc ggcttgcaca gccacgaggt cttggccgcg gccggcccgg cggccgccag
     1141 acacgcgaag gcggttacgg gcaggaggaa cagcgctggg cggggcatcg ccgcacccta
     1201 gtcgagcgtc acgcgatcgc cgatggcgat cgcgcctgat ccgcgtgccc gggcgttgat
     1261 cccgaacctc cccgtgtggc ggtcgaacag gtggcgcagc agcggcgccc acttctcctg
     1321 ggtgtcgggg tggcgtgtgg gtatggcgca ccgctcgcac gggtgcagca gggccagctc
     1381 ggccgagccc acccgcagcg tccggccctc ccagccctcc tcggcgaaag catcggcgtc
     1441 cagctccacg tggatgttgg tcctgaaccg gcgcaggtcc agctcggcgc ccagctcggc
     1501 cgcgacggcg tcgagcgtgg actgcacggt gaccagcagc gagtcgggca ggtcctgctg
     1561 cagcgcgggg tcggagtgca gcgtgacgtc gcgccccagg tcggccgaga gcgcggcggg
     1621 caggcccggg tccgaccacg cccactcggt cccgtcgggc gcgcgcaggg cgggatcggc
     1681 caggtcgccg tcgtaggacg ccctccacgc cagcaggcgc ggagcctggc gtgcggtcag
     1741 gcggcggggc gctcccttgt gcacgtcgaa gaccgcgtgg gcgcggtcgc cggcggcgcc
     1801 gcggccgtcc accgcgagcg tgtccacggg ctcgccaccc atcgacttga cgggccagcg
     1861 gtgcagctgg gtcacggtgc cggcgacgct catcagcggc accgtacaat cggggcatgg
     1921 ccgaggagcc cgtcgaagtc gccgaacagc cccacgagga ggagagcgag gccccgcggc
     1981 tggacccgga ggtcgaggcg cggcgatccg cggccatgtc cttcatcaag cgcctgggcg
     2041 accccgtcct gaagagcagg gcgacgcccg tcgaccactt cgacgacgcc ctccgcggcc
     2101 aggtcgcgcg catggccggc ctgatggacg acgcgctcgg catcgggctt gcggcgcccc
     2161 agctgggcgt gtcccagcgc ctgctggtct accgggtcgg gccggatgcg ccgctgatcg
     2221 cgctggccaa ccccgagatc gagtggcaca gcgacgacca ggacgtcctc caggaggggt
     2281 gcctctcgct gcccggcgtg cagatggaca tcgagcggcc cctgtacgtg cgcgtgcgtg
     2341 cccgtgacga gcacggcgac gagcgcgtgg tcgaggcctc gggcctcgag gcgcgggtga
     2401 tccagcacga gatggaccat ctggacggcg tgctgatcct ggaccgcggc agccgcgagc
     2461 agcgcaagga ggccatgcgg gccatccgcg aggccgaaga gcgccgcgag cgcgccgcct
     2521 gagccttcct ccggacggcg gagcggcgct ggacggctgt cctaccggcc gggttagcgg
     2581 cgttgccgga ttgggcacaa cgcgccgtac cacgagcgag gagatggtgc gggatgtcag
     2641 tagtgcgcaa ggagtgcaag cagtgcgggg tccacacctt ccggtcgcag tgtgggaact
     2701 gcgggtccac cgagctccgc gccgtcgggg ccgagcccga gacgccggcg cggttcgtgc
     2761 gccgggaccg caatgccctg tcgggcatcg tggccgcgcg cggtctacgc tccggcggtg
     2821 tcctctgagc cgaccgtcgt acacaacccg gacgagaccc gtttcgagct gatcctcgac
     2881 ggcgagcgca tcgggctggt ggactaccgg cccgccggcc ccagcgtgat cgtggcccac
     2941 accgaggtcg accccggcca cgagggcgag gggctgggcg gcatcctggt ccaggccgcg
     3001 ctggagggca tccgtgacat gggcaagacc gtgatcccga cgtgcccgtt caccgccgcc
     3061 tacatcaagc gccaccccga gtgggccgag cacgtggccc ccgcgatgcg ggcccagttc
     3121 gagcgctgat taccccggag gtcatggccc cgggcccgcg ccccgggtag aacccctccc
     3181 atgaccgata ccccacgcaa cggcaccgag ctgatggagc agttcgtgcc cgcgtcgccg
     3241 ttcgccgccc tggtgggcct ggaggtcgcc gagctggggg acggaaccgc ccggctggcg
     3301 ctgccctacc ggcccgagct ggccacgatg ggccagacgg tgcacggggg cgcgatcgcg
     3361 accgcgctgg acaccgccgc gatggccgcg gcgtggtcgg gcgccgagct gcccgagcgc
     3421 ctggccggtg ccacggtgtc gatggcggtg gagtacgtct cggccgcgaa cggccaggac
     3481 ctggaagtga ccgcgacgct gctgcgccgc ggccggtcgc tgtgctcggt cgaggccgag
     3541 gcccgcagcc cggacggggt gctcgtggcc aaggcgctgg tgacctacaa gctgggctga
     3601 cgccctaccc cttcgcggcg gtctcgatct cctcgagctt gtcgtaggcg ggcagcgtca
     3661 ggaactcgac gaacgtgtcg ttcccgctca gcgcgaccgc ctcgaacagg gcgcgcgact
     3721 cctccggccg gccctcgcgc tcgaaccact cgtcgtcgcc gatctccttg cggatggtct
     3781 ccagctcctc accggccagc tcgcgcacca gctccggagt gaccgtgcgg ccgtcgtcca
     3841 gcgtggcctc gtggcggatc cactgccaga tctggccgcg tgagatctcg gccgtggccg
     3901 cgtcctccat cagcccgtag atggcggccg cgccgttgcc gcgcagccag ctggagatgt
     3961 actggatgcc tacgttcacg ttcagccgca ggcccttctc ggtgatctgg ccctcggtct
     4021 tgtcgatggc cagcaggtcg gcggccgcca cgtccacgtc ctcgcgctgc ttgtcgacct
     4081 ggttgtcgcg gtcgccgagc accttctcga actcgcccat ggccggctcg accgagtcgg
     4141 ggtgggcgac ccaggagccg tcgaagcccg cgccggcctc gcgccgcttg tcggacgcca
     4201 cggcgtccag cgccttctgc gcggcctcct cgtcggtccg cgacgggatc acggcgctca
     4261 tgccgcccat cgcgtgcgcg ccgcggcggt ggcaggtctt gaccagaagc tcggagtagg
     4321 cgtgcatgaa cggcacggtc atcgtcacgt ccacccggtc gggcgtgacg aactcgggac
     4381 gctgcttgaa ccgcttgatg gcgctgaaga tgtagtccca gcggccggcg ttcaggcccg
     4441 cggagtggtc gcgcagctcg tagaggatct cgtccatctg gaacgcggcg ggcagggtct
     4501 cgatcagcac ggtgccc
//
LOCUS       DASUPW010000575         2678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1796136, whole genome shotgun sequence.
ACCESSION   DASUPW010000575 DASUPW010000000
VERSION     DASUPW010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1796136"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFY44_13005"
     CDS             <1..324
                     /locus_tag="VFY44_13005"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5911405.1"
                     /translation="EPDALTVGASGAIFGLMGAAVIVMRARGMNPMESGLGLWIGLNL
                     LITFTIPGISIGGHLGGLVGGALAAFVMFDLRERVRVPEIAGFLLAGAIGVASVVGAI
                     VVSAG"
     gene            complement(321..1991)
                     /locus_tag="VFY44_13010"
     CDS             complement(321..1991)
                     /locus_tag="VFY44_13010"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HEX5911406.1"
                     /translation="MNLRACLGPALLAAGALFSLLSAPAAQARPPSVVRVGGPSLPSD
                     PKIAVVASSRSLAGRRFTVTGRRGKVVLRGRLKRAAGSAAPWGGAATADLSRIRKPGS
                     YRVKVGRLRSRAWVVSAGAYAPLLSRLTRIYAVNSDGREPSPLFGPAHLSDARIATGP
                     FAGRRFDLTGGWRDAGDNLKFTQTTGLAVAALNYAARMAPARAAQLRATSDVGVRWLL
                     KAHPAPGLFVAQVGDESAHQQSVRDPAGDDGGSGPGQGVRPAYPSTGAPAAGLAAAGL
                     ALAAERSTGGARDQMLAGAREWYAAGRADMGPVGAPGGFYDDRTGADNMAVGAAALWR
                     ATGDDAFQADAGAFLRGSQFQSGVDSFEVAGLAAADLCGVLGARPATDPAVRGTACNG
                     LRQAAQAARERYRTTAFGSPGAFSFGWVQDNAGSGAMAALAAKAGLLKGGRTIGAGAR
                     DYLLGRNPWGQSFVVGRSRREAGAPHHSAYLKGNPRRLLNGAVVGGPASAQSLKDSNM
                     KIARSRFTRFNSPAAIYEDRRDDYVTSEVGLAYSSYAILLVAALGPGA"
     gene            complement(2027..>2678)
                     /locus_tag="VFY44_13015"
     CDS             complement(2027..>2678)
                     /locus_tag="VFY44_13015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5911407.1"
                     /translation="GLLRIPERAPRDEARRALGVPEDGFAVALLGRVSDWKGQDVLAR
                     ALAEPALAEIGAVGVVAGDAIPGGDAGRRLDALARELGVQERLVRLGFREDVDTVLGA
                     ADAVAVPSTRPEPLGLVALEAGAAGLPVVASADGGVAEVVRDGVSGLLVAPRDPAALA
                     GALARLAGDPEEAARLGGEGARLVAAEHAPERVTDALQDLYDGLAGPPGAKFGGAL"
BASE COUNT          300 a         1047 c         1029 g          302 t
ORIGIN      
        1 gagcccgacg ccctgaccgt cggcgcctcg ggcgccatct tcggcctgat gggcgccgcg
       61 gtgatcgtga tgcgcgcgcg cggcatgaac cccatggaga gcgggctcgg gctgtggatc
      121 ggcctgaacc tgctgatcac cttcaccatc ccgggcatct cgatcggcgg ccacctgggc
      181 ggcctggtgg gcggcgccct ggccgcgttc gtgatgttcg acctgcgcga gcgcgtccgc
      241 gtgcccgaga tcgcgggctt cctgctggcc ggcgccatcg gcgtggccag cgtggtgggc
      301 gcgatcgtgg tctcggcggg ctaggccccg gggcccagcg cggccaccag caggatcgcg
      361 tacgaggagt aggccaggcc gacctcggac gtgacgtagt cgtcgcggcg gtcctcgtag
      421 atcgcggccg gagagttgaa gcgggtgaag cggctgcggg cgatcttcat gttcgagtcc
      481 ttgagcgact gcgcgctggc cgggccgccg acgaccgcgc cgttcagcag cctgcgcggg
      541 ttgcccttca ggtaggcgga gtggtggggc gcgccggcct cgcggcgcga gcggccgacc
      601 acgaagctct gcccccacgg gttgcgcccc agcaggtagt cacgggcccc ggcgccgatg
      661 gtgcgcccgc ccttgagcag cccggccttg gccgccagcg ccgccatggc gcccgagccg
      721 gcgttgtcct ggacccagcc gaagctgaac gctcccgggc tgccgaacgc ggtggtgcga
      781 tagcgctcgc gcgcggcctg ggccgcctgg cgcagcccgt tgcaggcggt cccccgcacg
      841 gccgggtcgg tggccggcct ggcgcccagc accccgcaca ggtcggctgc cgccagcccg
      901 gcgacctcga acgagtccac gccgctctgg aactgcgatc cgcgcaggaa cgccccggcg
      961 tcggcctgga aggcgtcgtc gccggtcgcg cgccagaggg cggcggcccc cacggccatg
     1021 ttgtcggcgc cggtgcggtc gtcgtagaag ccaccgggcg cgcccaccgg tcccatgtcg
     1081 gccctgcccg ccgcgtacca ctcgcgcgcg ccggccagca tctggtcgcg cgcgccgccc
     1141 gttgaccgct cggcggccag cgccaggccg gcggcggcca ggccggccgc cggcgcgccg
     1201 gtggacgggt aggcggggcg cacgccctgc cccggcccgg acccgccgtc gtcgccggcg
     1261 ggatcgcgga cgctctgctg gtgggcggac tcgtcgccca cctgggccac gaacaggccg
     1321 ggcgcggggt gggccttcag cagccagcgc acacccacgt cgctggtggc ccgtagctgc
     1381 gcggcccgcg ccggcgccat ccgggcggcg tagttcaggg cggccaccgc caggccggtg
     1441 gtctgggtga acttgaggtt gtcgccggca tcgcgccagc cgccggtcag gtcgaagcgc
     1501 cggccggcga agggtccggt ggcgatccgg gcgtcgctga ggtgcgccgg cccgaagagg
     1561 ggggagggct cgcgcccgtc gctgttgacc gcgtagatcc gcgtcagccg cgacagcagc
     1621 ggcgcgtagg cgccggcgct gaccacccac gcgcgtgagc gcagccggcc gaccttcacg
     1681 cggtagctgc cgggcttcct gatccggctc aggtcggcgg tggcggcgcc gccccaggga
     1741 gcggcggagc ccgcggcgcg cttcaggcgt ccccgcagca ccaccttgcc gcggcggccg
     1801 gtgaccgtga accggcggcc ggccagggac cggctcgagg ccaccaccgc gatcttggga
     1861 tcggacggca ggctggggcc gcccacgcgg accacgctcg ggggccgggc ctgggccgcc
     1921 ggtgcgctga ggagagagaa cagcgctccg gcggccagga gggccggccc gaggcaggca
     1981 cggaggttca tgcgggcgat cctcgcaccg gtcgggtggg cgcgcctcag agggcccccc
     2041 cgaactttgc gccgggcggt ccggcgagcc cgtcgtacag gtcctggagc gcgtcggtga
     2101 cgcgctccgg cgcgtgctcg gcggccacga gccgggcacc ctccccgccc agccgcgcgg
     2161 cctcctcagg gtcgcccgcc agccgcgcca gggccccggc cagggcggcg gggtcgcgcg
     2221 gggccaccag cagcccggag acgccgtcgc gcaccacctc ggcgacgccg ccgtcggcgc
     2281 tggccacgac cggcaggccg gcggcgccgg cctccaacgc caccagcccc agcggctccg
     2341 gcctggtcga gggcaccgcc acggcgtcgg ccgcccccag gacggtgtcc acgtcctcgc
     2401 ggaagcccag ccgcaccagc cgctcctgga cgcccagctc gcgggccagg gcgtccaggc
     2461 gccggcccgc gtccccgcca gggatggcgt ccccggccac cacaccgacg gcgccgatct
     2521 ccgcgagcgc gggctcggcc agcgcgcggg ccagcacgtc ctgccccttc cagtcgctca
     2581 cccgccccag gagcgccacc gcgaagccgt cctccggcac gcccagcgcg cgccgggcct
     2641 cgtcacgcgg agcccgctcg gggatccgca gcagcccg
//
LOCUS       DASUPW010000576         3345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_272582, whole genome shotgun sequence.
ACCESSION   DASUPW010000576 DASUPW010000000
VERSION     DASUPW010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272582"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2001
                     /gene="ftsH"
                     /locus_tag="VFY44_13020"
     CDS             <1..2001
                     /gene="ftsH"
                     /locus_tag="VFY44_13020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX5911408.1"
                     /translation="GRPLQLAVMANDPATNAPAPEKPAAPPERKGPSWKVDPAPDGRG
                     KPPEKPPMLPFSWRRFVVILAVLLALNYLFVAIFAPAEERKQISYTPNFIQQVEKGNV
                     KEISSKGETLTGDYKKKVDGVEHFKTEVPTFANTDQLAKLLQDNDVRVNAKPPQDRSL
                     FETILFSFGPTLLIIGLLVFFLRRAAQQAGGGGMIGQFGRSKARRQEGGEDTRVNFSD
                     VAGIDEAKEELSEIVDFLRKPEKYQKLGARIPRGVLLSGQPGTGKTLLAKAVAGEAAV
                     PFFSISASEFIEAIVGIGASRVRDLFQQAKDAAPAIIFIDELDAIGRSRSGGGGFSSG
                     GHDEREQTLNQILTEMDGFETGDGVVVLAATNRPEILDKALLRAGRFDRRVAVQAPDK
                     VGREMILKVHTRSVPLADDVDLTGLASSTPGMVGADLANVVNEAALIAAKREHPAVRQ
                     ADLTDALEKIVLGTARSVVQSEEDRRRIAYHEGGHAIVGMLTQGADPVRKVSIIPRGQ
                     ALGVTMSAPDADVFNYEEAYLRGKIKVALGGRVAEEITFGTITTGAESDIQQATQIAR
                     GMVARWGMSDEIGFVAVQSSGDDGPLLPGVSETSEATQQTVDAEVRRIIEEAHGDVRN
                     LLERERDKLDALAEALLREETLDEIDAYAAAGIGRAAAPAGA"
     gene            2006..2848
                     /gene="nadC"
                     /locus_tag="VFY44_13025"
     CDS             2006..2848
                     /gene="nadC"
                     /locus_tag="VFY44_13025"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX5911409.1"
                     /translation="MERLIATALAEDVGSGDLTVAAVVPAGTRARAIVSQKEPGVISG
                     LEVAREVFRQVDPVLTFDALGPEGEWREGGEVAAIEGPAGSILTGERTALNFLGRLSG
                     VATATARHVRAVQGTGARILDTRKTTPGLRALEKQAVLHGGGVSHRGGLYDAILVKEN
                     HAALAGGIVPAARAALAASEGAAFVELEVESLDELDQALAEGVDRILLDNMGPDELRE
                     AVARCAGRASLEASGGITLETIGAVARTGVDYISVGALTHSAPALDLSLLLDPGAAAP
                     RTGR"
     gene            complement(2799..>3345)
                     /locus_tag="VFY44_13030"
     CDS             complement(2799..>3345)
                     /locus_tag="VFY44_13030"
                     /inference="COORDINATES: protein motif:HMM:NF040269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5911410.1"
                     /translation="TLAESIRAQLATKVDVRELAWLEQLETLSAPDRHPGEWQLATAY
                     MGIVPADVDPALPGDTRWVAVDELPDALAFDHGPIVLAGRERLRAKLSYTNIGFALAP
                     QTFTISELRDVYSAALGHGVSATNLQRVLERRGLLEPTGERRSPGPGGGRPAAVYRFG
                     SRSVEVTDPFAVLRPPGRAEG"
BASE COUNT          497 a         1183 c         1250 g          415 t
ORIGIN      
        1 ggacggcccc tacagttggc ggtcatggcc aacgacccag caaccaacgc cccggcgccc
       61 gagaagccgg ccgcgccgcc cgagcgcaag gggccgagct ggaaggtcga ccctgcgccc
      121 gacggccgcg gcaagccgcc cgagaagccg ccgatgctgc cgttcagctg gcggcggttc
      181 gtggtcatcc tggccgtgct gctggcgctg aactacctgt tcgtcgccat cttcgccccg
      241 gcggaggagc gcaagcagat ctcctacacg cccaacttca tccagcaggt cgagaagggc
      301 aacgtcaagg agatctcctc caagggggag acgctcaccg gcgactacaa gaagaaggtc
      361 gacggggtgg agcacttcaa gaccgaggtc cccaccttcg ccaacaccga ccagctggcc
      421 aagctgctcc aggacaacga cgtgcgggtg aacgccaagc cgccccagga ccggtcgctg
      481 ttcgagacca tcctgttctc gttcggcccg acgctgctga tcatcggcct gctggtgttc
      541 ttcctgcgcc gcgccgccca gcaggcgggc ggcgggggca tgatcggcca gttcgggcgc
      601 tcgaaggccc ggcgccagga gggcggggag gacacgcgcg tgaacttctc ggacgtggcc
      661 gggatcgacg aggccaagga ggagctgtcg gagatcgtcg acttcctcag gaagcccgag
      721 aagtaccaga agctgggcgc ccggatcccg cgcggcgtgc tgctgtcggg ccagccgggc
      781 acgggcaaga cgctgctggc caaggccgtg gccggtgagg ccgcggtgcc gttcttctcg
      841 atctcggcct ccgagttcat cgaggccatc gtcggcatcg gcgcgtcacg ggtgcgcgac
      901 ctgttccagc aggccaagga cgccgcgccg gccatcatct tcatcgacga gctggacgcc
      961 atcgggcggt cgcgctcggg cggcggcggc ttctccagcg gcggccacga cgagcgcgag
     1021 cagacgctga accagatcct gaccgagatg gacgggttcg agaccggcga cggggtggtc
     1081 gtgctggccg ccaccaaccg gcccgagatc ctggacaagg cgctgctgcg cgccgggcgc
     1141 ttcgaccgcc gcgtcgccgt gcaggcgccc gacaaggtcg gccgcgagat gatcctgaag
     1201 gtgcacacgc ggtcggtgcc gctggccgac gacgtggacc tgacgggcct ggcctcgtcc
     1261 acccccggca tggtgggggc cgacctggcc aacgtggtca acgaggcggc gctgatcgcc
     1321 gccaagcgcg agcatcccgc ggtgcgccag gcggacctga cggacgcgct ggagaagatc
     1381 gtgctgggca ccgcgcgcag cgtggtccag tccgaggagg accggcgccg gatcgcctac
     1441 cacgagggcg ggcacgccat cgtcggcatg ctcacccagg gcgcggaccc ggtccgcaag
     1501 gtctcgatca tcccccgcgg ccaggccctg ggcgtgacca tgtcggcccc cgacgccgac
     1561 gtgttcaact acgaggaggc ctacctgcgc ggcaagatca aggtcgcgct gggcggccgg
     1621 gtggccgagg agatcacgtt cggcacgatc accaccggcg ccgagtccga catccagcaa
     1681 gccacccaga tcgcccgcgg gatggtcgcg cgctggggca tgagcgacga gatcggcttc
     1741 gtcgccgtgc agtcctcagg cgacgacggg ccgctgctgc ccggcgtcag cgagacctcc
     1801 gaggccacgc agcagaccgt ggacgccgag gtgcggcgca tcatcgagga ggcgcacggc
     1861 gacgtgcgca acctgctcga gcgcgagcgc gacaagctcg acgccctggc cgaggcgctg
     1921 ctgcgcgaag agacgctgga cgagatcgac gcctacgcgg cggccggcat cggccgcgcg
     1981 gcagcgcccg ccggcgcctg acgtcatgga gcggctgatc gccacggctt tggccgagga
     2041 cgtcggctcc ggcgacctga cggtcgcggc ggtcgtgccc gcgggcaccc gggcgcgggc
     2101 gatcgtcagc cagaaggagc ccggggtgat ctccggcctt gaggtggcgc gcgaggtgtt
     2161 ccgccaggtc gacccggtcc tgaccttcga cgcgctcggc cccgaggggg agtggcgcga
     2221 ggggggagag gtggcggcga tcgagggccc cgcggggtcg atcctcacgg gtgagcgcac
     2281 cgccctgaac ttcctgggcc gcctgtccgg cgtggccacc gccaccgccc gccacgtccg
     2341 ggccgtccag ggcaccggcg cccggatcct ggacacgcgc aagaccaccc cggggctgcg
     2401 ggcgctggag aagcaggcgg tcctgcacgg gggcggggtg tcccaccgcg gcggcctgta
     2461 cgacgcgatc ctggtcaagg agaaccacgc cgcgctggcc ggcggcatcg tgcccgccgc
     2521 ccgggccgcg ctggccgcat ccgagggcgc cgcgttcgtc gagctggagg tcgagagcct
     2581 ggacgagctc gaccaggcgc tggcggaggg ggtggaccgg atcctgctgg acaacatggg
     2641 cccggacgag ctgcgcgagg ccgtggcccg gtgcgccggg cgcgccagcc tcgaggcgtc
     2701 gggcggcatc accctggaga ccatcggcgc ggtggcccgg accggcgtcg actacatctc
     2761 ggtgggggcg ctgacgcact cggcgccggc gctggacctc agccttctgc tcgacccggg
     2821 ggccgcagca ccgcgaacgg gtcggtgacc tccaccgacc gcgagccgaa gcggtagacg
     2881 gccgccggcc ggccgcctcc tggccccggc gagcgccgct ccccggtcgg ctccagcagg
     2941 ccgcgccgtt cgagcacccg ctgcaggttg gtggccgaga cgccgtggcc gagcgcggcc
     3001 gagtacacgt cccgcagctc ggagatcgtg aacgtctgcg gagccagcgc gaagccgatg
     3061 ttggtgtacg agagcttcgc gcgcaggcgc tcccggccgg cgagcacgat gggcccgtgg
     3121 tcgaaggcca gcgcgtccgg cagctcgtcg acggccaccc agcgggtgtc gccgggcagc
     3181 gccgggtcca cgtcggcggg cacgatgccc atgtaggccg ttgccagctg ccactcgccg
     3241 gggtggcgat cgggggcgct cagggtctcc agctgttcga gccaggccag ctcgcgcacg
     3301 tccaccttgg tggccagctg ggcgcggatc gactccgcaa gggtc
//
LOCUS       DASUPW010000577         7811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_272840, whole genome shotgun sequence.
ACCESSION   DASUPW010000577 DASUPW010000000
VERSION     DASUPW010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7811
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272840"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /gene="atpB"
                     /locus_tag="VFY44_13035"
     CDS             complement(<1..893)
                     /gene="atpB"
                     /locus_tag="VFY44_13035"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX5911411.1"
                     /translation="MGTKGKVGIAFALLLAMAVGLFGIFGSEGRNEEFQPQNEFKLDP
                     WINLKLGPLDMSINKAVLYVFLAAALATGIMIFISRRMTQRPNNVQTAVEAAYEFSAN
                     QIVGANIEDRRLAKKWFPFIATLFFFILFSNLIGYIPLPTNTEHPIHIGGLEIPAFAI
                     YAATANVSIPLILTLMVVCIYHYEGVKAKGPVNYIKGWIPAGTPGGLLFLILPIEIIS
                     QFVRIISLSVRLFANLLAGHLLILFMGGGLVVILGLAAVGVFTLPVAIAFFMFEVVLI
                     AGLQAFIFATLTSIYLGEAVA"
     gene            complement(893..1333)
                     /locus_tag="VFY44_13040"
     CDS             complement(893..1333)
                     /locus_tag="VFY44_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911412.1"
                     /translation="MTTLALNTQTPAAAPGRGLAVLRYLDLIVLALALPFFIAFSLPL
                     LGYAVIAAVWIIQRVVQHFANAKAEASNDPRTSVGVLAGSMLGRGWLTAGAILAVGLI
                     GTRPDGLAAAVMAISLFTIYFAAQMVVRPFDEAARKPLPPKESR"
     gene            complement(1427..2998)
                     /locus_tag="VFY44_13045"
     CDS             complement(1427..2998)
                     /locus_tag="VFY44_13045"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5911413.1"
                     /translation="MAQGAAWFAAVAALLGIAVVALPLAPEADQLGLAATALACGILA
                     AVALVGGGRLPTWYFASGAVVGTLVAGAATHYWGEGSFVGALPFLFPVLYAAWFLPRT
                     VTLAVLVLAAALLAAELIDQDRGDAAVGAWVATTGTLAGAGVLVALLRRRITEAVASL
                     TEAARRDPLTGLLNRRGFEEVFDVELERARRTEQSLSVVVGDLDRFKRINDEHGHAAG
                     DNALRRVGRVLGEGKRSWDIAARVGGEEFAILTPDTDEHGAYILAERLRTMIAGEFAG
                     DQPDPLTISFGIVSYPVHGQTGSSLLQAGDQALYAAKRLGRNRSVISSAEVPGILSGT
                     GGARNHEDTSVELTSLLSLAEALDVRDNGSPTHCRRVGRYSELIARELGLPPDSVERV
                     RIAGVLHDVGRVGVPDELLAKEGPLDEEEWHWVREHPEIGARMLETTDFADIGSWIRS
                     HHERPDGSGYPEGRSGELPLEAAILGVADAYEAMTAPRPYRDALDPQAASDELRRGSG
                     RQFDERVVDALLRVV"
     gene            3221..3637
                     /locus_tag="VFY44_13050"
     CDS             3221..3637
                     /locus_tag="VFY44_13050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX5911414.1"
                     /translation="MIEQTEITLEARPRGFHLITDEVLAGAPDLGDVEAGLLHLWMRH
                     TSASLTVNENASPDVRRDFESWANEAVPEDASYWTHTFEGPDDMPAHIKASLMGPSLT
                     LPVSGGALALGTWQGIYLCEHRDRGGARGVVATLMA"
     gene            3837..4832
                     /locus_tag="VFY44_13055"
     CDS             3837..4832
                     /locus_tag="VFY44_13055"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5911415.1"
                     /translation="MRWSDQQLEAEREHTLPGYREPASVRTFDAPEALDTRFYEVRAK
                     SALNRVPKASQMPFRWTVNPYRGCSHACVYCFARPTHTYLDLDAGRDFEKEIVVKVNA
                     PEVLRGELARPSWRREHVAMGTNTDPYQWVEGRYRLMPEIWRALADSNTPASVLSKSP
                     LLLRDVEEMKRVPRISANLSIPTLDEKEWRSTEPHTPHPRKRMEAVAELNRQGIPTGI
                     LIAPLMPGVNDSPAQVERILELAEEAGAVSVGGIALHLRGEVRGIFFDWLRSQRPDLV
                     PRYERLYARGAYAPRAERERIARLTRGRGRRFLRPAPTDEPPPPPPSQPAQPTLF"
     gene            4847..6445
                     /locus_tag="VFY44_13060"
     CDS             4847..6445
                     /locus_tag="VFY44_13060"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX5911416.1"
                     /translation="MARPIDRREFMGQAGFGAGALILGGIAVDPAIAAKRRTRRRGPQ
                     LAREGAFPQGVAAGTPAERGISLWTRMEGQAGDFKLRVEIATDPKFRKVVRRTTKTVR
                     ARKDHCLETRMAGKFLKPGQEYWYRFETRDGSSPVGRFQTSPAADSRAPVKIVFFSCQ
                     DWQAGYYAAHRAIAAEDADLVVCLGDYIYERNFYDGPRKDTLGANRDGEVQTLAEYRD
                     KYRQYKADPDLRAMHAQHAYVGIWDDHEVEDNYAGSNPGEETQQVRRPFLNRKASAYR
                     AFYEYMPFEPLYGQPEVGSDLYRRMRLGANVELFLLDERQDRDDQPCDDAFFTPCAGA
                     ENQPRRFLGNRQKEWLKRRLRASGASWKVIGNQLMIMSLELVQGAQIVKDSWDGYGVE
                     RREILDFVRARGIDNVSFLTGDIHTFFAGDVGVNGRGPGSVATEFVGGSVTSLGIPEL
                     VQGTTGVPLTGEQSTLLTNNLLVTNPHIKYTQQTSRGYGVAEISRDEMRVTFKGVNAL
                     DPKATARTIGRFRVASGSPRVQVL"
     gene            complement(6422..7591)
                     /locus_tag="VFY44_13065"
     CDS             complement(6422..7591)
                     /locus_tag="VFY44_13065"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX5911417.1"
                     /translation="MNGLDALWAFLVAGAVTVLLTPLAARLAHRVGAIDQPRERSLHE
                     FPTPSLGGLAILAGVLVAGLLFLPFNTETKGILGGAVLIALVGAADDVAPDGMSALPK
                     LLGQVAAAGVVVACNVRVENLTLPFIEPLDLGNWGIPLTLFGIVAVMNVVNFTDGVDG
                     LAAGVCSIAAVTFAIIALSLDRDDAAVLAAVVAGASIGFLRHNFHPASVFMGDSGSNL
                     LGLLLACVAIQGVLKTAAVVALFFPLVILAVPALDATFVVAKRLKYRRPVYSADRWHF
                     HHRMANIGFSTRRTVLYLYAWTVSLAALALALRFVPYSQDDGQLNPGWALVMLAFGLA
                     ALAASFYLVYVLELFKFRRIRERQMRTREEIERELETGEFEAVERGSRSEDLDAR"
BASE COUNT         1236 a         2756 c         2782 g         1037 t
ORIGIN      
        1 tcggccactg cctcgccgag gtagatagag gtcaaggtgg cgaagatgaa ggcctgtagg
       61 ccggcgatca ggaccacctc gaacatgaag aacgcgatgg ccacgggcag cgtgaacacg
      121 ccgaccgccg ccaggccgag gatcacgacc agcccgccgc ccatgaacag gatcagcagg
      181 tggccggcca gcaggttggc gaacaatcgc accgacagcg agatgatccg cacgaactgc
      241 gagatgatct cgatcggcag gatcaggaac agcagcccgc cgggcgtgcc cgccgggatc
      301 cagcccttga tgtagttgac cgggcccttc gcctttacgc cctcgtagtg gtagatgcac
      361 accaccatca gggtcaggat cagggggatc gacacgttgg ccgtcgccgc gtagatcgcg
      421 aacgccggga tctcgaggcc cccgatgtgg atggggtgct cggtgttggt cggcaacggg
      481 atgtagccga tcaggttgga gaagaggatg aagaagaaga gcgtggcgat gaacgggaac
      541 cacttcttgg ccaggcgccg gtcctcgatg ttggcgccga cgatctggtt cgcggagaac
      601 tcgtaggcgg cctcgacggc cgtctggacg ttgttcgggc gctgcgtcat gcgcctgctg
      661 atgaagatca tgatccccgt ggccagcgcg gcggccagga acacgtagag caccgccttg
      721 ttgatgctca tgtccagcgg gccgagcttc aggttgatcc agggatcgag cttgaactcg
      781 ttctggggct ggaactcctc gttacggcct tcggagccga agatgccgaa cagcccgacc
      841 gccatggcca gcagcagcgc gaacgcgatc ccgaccttgc ccttggttcc catcagcggc
      901 tctccttagg cggcagcggc ttgcgcgcgg cctcgtcgaa cgggcgcacg accatctgcg
      961 ccgcgaagta gatcgtgaac agcgagatgg ccatgaccgc ggcggccagc ccgtccgggc
     1021 gggtgccgat caggccgacg gccaggatcg ccccggcggt gagccagccg cggccgagca
     1081 tgctgccggc cagcacgccg acgctggtgc gcgggtcgtt gctggcctcg gccttggcgt
     1141 tggcgaagtg ctggacgacg cgctggatga tccacaccgc ggcgatcacc gcgtagccca
     1201 gcagaggaag cgagaaggcg atgaagaagg gcagggccag cgccagaacg atcaggtcga
     1261 ggtaccgaag gacggctaga cctctaccgg gcgcggctgc gggggtttgt gtgttcaggg
     1321 cgagcgtcgt caaggggttc cctggcgggc gaaagactgt atccgaggcc aagcgagagt
     1381 gactacttgg tgacgcctgc cgcgaggccg gcgtgactcc cggcggtcag actacccgga
     1441 gcagggcatc gaccacccgc tcgtcgaact ggcggcccga gccgcggcgc aactcgtcgg
     1501 acgccgcctg cggatccagc gcgtcgcggt agggccgggg ggccgtcatc gcctcgtacg
     1561 cgtccgccac gcccaggatg gccgcctcga gcggcagctc gccgctgcgg ccctcggggt
     1621 agccgctgcc gtcgggccgc tcgtggtggc tgcggatcca gctgccgatg tcggcgaagt
     1681 cggtggtctc gagcatccgg gccccgatct ccgggtgctc gcgcacccag tgccactcct
     1741 cctcgtccag cgggccctcc ttggccagca gctcgtcggg cacgcccacg cggccgacgt
     1801 cgtgcagcac gccggcgatg cgcacgcgct cgaccgagtc cggcggcagg ccgagctcgc
     1861 gcgcgatcag ctctgaatag cggccgacgc ggcggcagtg ggtggggctg ccgttgtcgc
     1921 ggacgtccag cgcctcggcc agcgagagca ggctggtcag ctccaccgac gtgtcctcgt
     1981 ggttgcgggc gccgccggtg ccgctgagga tgcccggcac ctcggcgctg gagatcaccg
     2041 agcggttgcg gcccagccgc ttcgcggcgt acagggcctg gtcgccggcc tggagcagcg
     2101 acgagccggt ctggccgtgc accgggtagc tgacgatgcc gaagctgatg gtcagcgggt
     2161 cgggctggtc gcccgcgaac tcgcccgcga tcatggtgcg gaggcgctcg gccaggatgt
     2221 acgcgccgtg ctcgtcggtg tccggcgtca ggatcgcgaa ctcctcgccc cccacccgcg
     2281 cggcgatgtc ccacgagcgc ttgccctcgc ccagcacgcg gccgacccgg cgcagcgcgt
     2341 tgtcgccggc cgcgtggccg tgctcgtcgt tgatgcgctt gaagcggtcc aggtcgccga
     2401 cgaccacgct gaggctctgc tcggtccggc gcgcgcgctc gagctcgacg tcgaacacct
     2461 cttcgaagcc gcgccggttc agcaggccgg tcagtgggtc gcggcgcgcg gcctcggtca
     2521 ggctcgccac cgcctcggtg atccgccggc gcagcagcgc caccagcacg ccggcgccgg
     2581 ccagcgtgcc ggtcgtggcc acccacgcgc cgacggcggc gtcgccccgg tcctgatcga
     2641 tcagctcggc ggccagcagg gcggcggcca ggaccaggac cgcgagggtc acggtgcggg
     2701 gcaggaacca ggcggcgtag agcaccggga acaggaacgg cagggcgccg acgaacgagc
     2761 cctcgcccca gtagtgggtg gccgcgcccg cgaccagggt ccccaccacc gcgccgctcg
     2821 cgaagtacca ggtgggcagg cgcccgccgc ccacgagcgc gaccgcggcc aggatgccgc
     2881 aggccagcgc ggtggcggcc aggccgagct ggtcggcctc gggggccagc ggcagcgcga
     2941 ccacggcgat gcccagcagc gcggccaccg cggcgaacca cgcggcaccc tgggccatcg
     3001 ccacccggct accgggcacg gcgatccccg catctaccga agtgcgccag ccgaagcgct
     3061 ggcggaggcc ggcccgcatc gtgcttctcc tgtctctcta ccggttgttc catcgcacga
     3121 tccgagtcgc tctcgagggc ggtcgcgacc gtgtgagaca ggcgtgcctg cccggaatat
     3181 cttgcttcag caatatagga gcgacctacg aatcccgcca atgatcgagc agaccgaaat
     3241 caccctcgag gcccgcccgc gcggcttcca tctgatcacc gacgaggtgc tggccggggc
     3301 ccccgatctg ggtgacgtcg aggccggcct cctgcacctc tggatgcggc acacctcggc
     3361 ctcgctgacc gtcaacgaga acgcctcccc cgacgtgcgg cgcgacttcg agtcgtgggc
     3421 gaacgaggcc gtgcccgagg acgcctccta ctggacccac acgttcgagg gccccgacga
     3481 catgccggcc cacatcaagg cgtcgctgat gggcccgtcg ctgaccctgc cggtgtccgg
     3541 cggggcgctg gcgctcggga cgtggcaggg gatctatctg tgcgagcacc gcgatcgggg
     3601 tggggcgcgg ggggtcgtgg cgacgttgat ggcgtaggcg ccggaagcgt tcgcctgcaa
     3661 ccgtcccgtt accgaccagc gtgccgtcct cgggttgtgc gcagttgacc cccgtatagg
     3721 gggcaaagtg cgcacggctc caccccggtg ggcggcggac ggtgacggga gggttgcgag
     3781 cgttacggcc gggcgggagg ccgattccgc cgccgcctat gcgaacatgt gttcgtatgc
     3841 ggtggtccga tcagcagctc gaggccgaac gggagcacac cctccccggc taccgcgagc
     3901 cggcgtcggt ccggacgttc gacgcgcccg aggcgctgga cacccggttc tacgaggtac
     3961 gcgcgaagtc ggcgctgaac cgggtgccga aggcgtcgca gatgccgttc cggtggacgg
     4021 tgaacccgta ccggggttgc agccacgcct gcgtgtactg cttcgcccgc cccacccaca
     4081 cctacctcga cctggacgcc ggccgtgact tcgagaagga gatcgtggtc aaggtcaacg
     4141 cgcccgaggt gctgcgcggc gagctggcgc ggccgtcgtg gaggcgcgag cacgtggcca
     4201 tgggcaccaa caccgacccc tatcagtggg tcgagggccg ctaccggctg atgccggaga
     4261 tctggcgcgc gctggccgac tcgaacacgc ccgcctcggt gctgtccaag tcgccgctgc
     4321 tgctgcgcga cgtcgaggag atgaagcggg tgccccggat cagcgccaac ctgtcgatcc
     4381 ccacgctgga cgagaaggag tggcggtcca ccgagccgca tacgccccac ccgcgcaagc
     4441 ggatggaggc ggtggccgag ctcaaccgcc agggcatccc gaccgggatc ctgatcgcgc
     4501 cgctcatgcc cggggtcaac gactcgcccg cccaggtgga gcggatcctg gagctggccg
     4561 aggaggccgg cgcggtcagc gtcggcggca tcgcgctgca cctccggggc gaggtccggg
     4621 ggatcttctt cgactggctg cgctcgcagc gcccggacct ggtgccgcgg tacgagcgcc
     4681 tgtacgcgcg cggcgcctac gcgccgcggg ccgagcgcga gcggatcgcc aggctgaccc
     4741 gcgggcgcgg ccggcggttc ctgcgccccg cccccaccga cgagccgcca ccgccgccgc
     4801 cttcacaacc tgcgcagccc accctgttct gacgtacgct cggcgggtgg cgaggcccat
     4861 agaccggcgg gagttcatgg gccaggccgg cttcggcgcc ggcgcgctga tcctgggagg
     4921 catcgcggtg gatccggcga tcgccgccaa gcggcgcacg cgccggcgcg ggccgcagct
     4981 tgcgcgcgag ggcgccttcc cccagggcgt ggcggccggc acgccggccg agcgcggcat
     5041 ctcgctgtgg acgcgcatgg agggccaggc cggcgacttc aagctgcgcg tcgagatcgc
     5101 caccgacccc aagttccgca aggtcgtccg gcggacgacg aagacggtcc gcgcgcgcaa
     5161 ggaccactgc ctggagacgc ggatggcggg caagttcctc aagcccggcc aggagtactg
     5221 gtaccgcttc gagacccgcg acggctcgag cccggtgggc cggttccaga cctcgcccgc
     5281 cgcggactcg cgcgcgccgg tgaagatcgt cttcttctcc tgccaggact ggcaggcggg
     5341 ctactacgcg gcgcaccggg ccatcgccgc cgaggacgcc gacctggtgg tgtgcctggg
     5401 cgactacatc tacgagcgca acttctacga cgggccgcgt aaggacacgc tgggcgccaa
     5461 ccgcgacggc gaggtccaga cgctggccga gtaccgcgac aagtaccgcc agtacaaggc
     5521 cgaccccgac ctgcgggcca tgcacgccca gcacgcgtac gtgggcatct gggacgacca
     5581 cgaggtcgag gacaactacg ccggctccaa ccccggcgag gagacccagc aggtgcgccg
     5641 gccgttcctg aaccgcaagg ccagcgcgta ccgcgcgttc tacgagtaca tgccgttcga
     5701 gcccctgtac ggccagcccg aggtgggcag cgacctgtac cggcgcatgc gcctgggcgc
     5761 gaacgtcgag ctgttcctgc tcgacgagcg ccaggaccgc gacgaccagc cctgcgacga
     5821 cgcgttcttc accccctgcg cgggggccga gaaccagccg cgccggttcc tgggcaaccg
     5881 ccagaaggag tggctcaagc ggcggctgcg ggcctccggc gccagctgga aggtgatcgg
     5941 caaccagctg atgatcatgt cgctggagct ggtccagggt gcgcagatcg tcaaggacag
     6001 ctgggacggc tacggggtcg agcggcgcga gatcctggac ttcgtccgcg cgcggggcat
     6061 cgacaacgtc agcttcctga ccggcgacat ccacaccttc ttcgccggcg acgtgggcgt
     6121 gaacggccgc gggccgggca gcgtggccac cgagttcgtc ggcggttcgg tcacttcgct
     6181 gggcatcccg gagctggtgc agggcaccac cggcgtgccg ctcaccggcg agcagtcgac
     6241 actgctgacc aacaacctgc tggtgaccaa cccgcacatc aagtacaccc agcagacctc
     6301 gcgcggctac ggcgtggccg agatcagccg cgacgagatg cgcgtgacct tcaagggcgt
     6361 gaacgcgctc gaccccaagg ccacggcgcg cacgatcggg cgcttccgcg tggccagcgg
     6421 ctcaccgcgc gtccaggtcc tctgagcgcg atccgcgttc cacggcctcg aactcgccgg
     6481 tctcgagctc gcgctcgatc tcctcgcggg tgcgcatctg gcgctcgcgg atgcgccgga
     6541 acttgaacag ctcgagcacg tagaccaggt agaagctggc ggccagcgcc gccagcccga
     6601 aggcgagcat gaccagcgcc cagcccgggt tcagctggcc gtcgtcctgg gagtagggca
     6661 cgaaccgcag cgccagcgcc agcgcggcca gcgacacggt ccaggcgtac aggtacagca
     6721 ccgtgcgccg ggtcgagaag ccgatgttgg ccatccggtg gtggaagtgc cagcggtcgg
     6781 ccgagtacac gggccggcgg tacttcagcc gcttggccac cacgaaggtc gcgtccagcg
     6841 cgggcacggc caggatcacg agcgggaaga acagggccac gacggcggcg gtcttcagca
     6901 cgccctggat ggccacgcag gccaggagca acccgagcag gttcgagccc gagtcgccca
     6961 tgaagaccga ggccgggtgg aagttgtggc gcaggaagcc gatcgaggcg ccggcgacca
     7021 cggcggccag cacggccgcg tcgtcgcggt ccagcgacag ggcgatgatc gcgaacgtga
     7081 ccgccgcgat gctgcacacg ccggcggcca gtccgtcgac gccgtcggtg aagttcacga
     7141 cgttcatcac ggcgacgatc ccgaacagcg tcagggggat cccccagttg cccaggtcca
     7201 ggggctcgat gaacggcagc gtcaggttct cgacccgtac gttgcaggcc accaccacgc
     7261 cggccgcggc cacctgaccc agcagcttgg gcagcgccga catgccgtcg ggcgccacgt
     7321 cgtcggccgc gcccaccagg gcgatcagca ccgcgccgcc caggatgccc ttcgtctcgg
     7381 tgttgaaggg caggaacaac aggcccgcca ccaggacccc ggccaggatg gccaatccgc
     7441 ccaggctggg cgtggggaac tcgtgcaggg agcgctcgcg cggctggtcg atcgcaccga
     7501 cgcggtgggc cagcctcgcc gccagcgggg tcagcaggac cgtcacggcg ccggcgacca
     7561 ggaacgccca gagcgcgtcg aggccgttca tccgcgccca cactaccccg caccccggcc
     7621 gcgactatcg gccccgacgc ggtttgtacg ctgcattccc cccgtcggac ccaaggagag
     7681 aactgcatga ccccgaatcg cctggcccgc tgggccctgg ccatctgcgc cgcggccgtt
     7741 gccgccgcgt cgttcgcccc cgccgcatcc gcggccaaga agaagcagaa ggacccggat
     7801 cccaccctga a
//
LOCUS       DASUPW010000578         2747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_2074599, whole genome shotgun sequence.
ACCESSION   DASUPW010000578 DASUPW010000000
VERSION     DASUPW010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2074599"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..441
                     /locus_tag="VFY44_13070"
     CDS             28..441
                     /locus_tag="VFY44_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911418.1"
                     /translation="MPSLASRKLTTALTTAALLLAAPAAAMAQGAGDDQYVDPLGNSP
                     SDQGSGDTNSGGNSGSGNSGSGNSGNSGSGTAAPATPSQSQDPAQTQTTTPTPQAQSG
                     QELPRTGYPAWLAALAGGVLLASGATLRVSLGLRR"
     gene            complement(354..1871)
                     /locus_tag="VFY44_13075"
     CDS             complement(354..1871)
                     /locus_tag="VFY44_13075"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX5911419.1"
                     /translation="MSGRSERLHFPREELRGRTIRGGVLNALFLGGGEALALVQGLVV
                     TALLGPVAIGLYGIVTTTAMTMVQLRRVGIDEAFVQQDEEGQEEEFQRAFSLELAIGV
                     AFSFLLALLAPVVALVYGDMRLLALTLAVSYLPIAFALQAPQWIFFRRMDFLRLRLLQ
                     FSIPAVTFLVTVPLAAVTGSVWAMVIGPAAGNLVAVAAALKLSPYRLALRFERQAMRR
                     YLAFSWPILFTALATLIVLQGQIFAFDLKGGLAAAGFITVAVTLTRYADRADQIVTTT
                     IYPAICEVVDDLAIQAELFVKSSRVTMIWALPFCAGLALFAADLVDHVLGAEWEPAVI
                     LIQGLAVAAALQQIGFAWISFYRARGESWVQAVESAAMLGGFLLLAVPGLIVWDSKGF
                     VAGRVATSVAMLVVRRHYVRALFPEVRFVMMGLRGALPVLAGTGATLALRAALDPGTV
                     PELLVFLGVTFGAIWGADRPLLTELLRYLRRPNETRSVAPDASSTPPANAASHAG"
     gene            complement(1868..>2747)
                     /locus_tag="VFY44_13080"
     CDS             complement(1868..>2747)
                     /locus_tag="VFY44_13080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5911420.1"
                     /translation="TSTLVTVAVCTRDRPGELAETLDALTREASEQVEVLVVDQSPAE
                     DPALARRDDVRVIRDPGRGLSRARNVALAAVESRWIAFVDDDCTVAPGFGAALQAQLR
                     AAGDADWLSGHVGGGDREDGGLPLVTTFPVPRPAVRQGRWVLPGSIGFGVMFVVRCAT
                     ARALGGWDERLGPGVQPFPAADDMDFNHRLLRSGAKAVLTPEVRATHRQWRSPAELVD
                     LNRGYSRAWAGFAAKQARTGHPATALWLWGWGLVDVLDMTKSALGRRSRLRARMAGAK
                     LRGLAEGFAAGIGRRW"
BASE COUNT          422 a         1096 c          945 g          284 t
ORIGIN      
        1 cggcgctagc tactagggtt ccgccccatg ccttcacttg cctcgcgaaa gctcaccacc
       61 gccctcacca ccgccgccct cctgctggcg gccccggccg ccgcgatggc gcagggggcg
      121 ggcgacgacc agtacgtcga cccgctgggc aactcgccca gcgaccaggg ctccggcgac
      181 accaacagcg gcggcaactc gggatccggc aactcgggct cgggcaactc cggcaattcg
      241 ggctcgggca cggccgcccc ggccacgccc agccagtcgc aggaccctgc gcagacccag
      301 accaccactc cgacgcccca ggcccagagc ggccaggagc tgccgcggac cggctacccg
      361 gcgtggctgg cggcgttggc cggcggcgtg ctgctggcgt ccggcgcgac gctgcgggtc
      421 tcgttgggcc tgcgcaggta gcgcagcagc tcggtcagca gcgggcggtc ggcgccccag
      481 atggcgccga acgtgacccc caggaacacc agtagctcgg gcaccgtgcc cgggtccagc
      541 gccgcgcgca gcgcaagcgt ggcgcccgtc cccgccagca ccggcagggc gccgcgcagc
      601 cccatcatca cgaaccggac ctcggggaac agcgcccgca cgtagtggcg gcggaccacc
      661 agcatggcca ccgaggtggc cacccgcccg gccacgaacc ccttgctgtc ccagacgatc
      721 agcccgggca cggccagcag caggaagccg ccgagcatcg cggcggactc caccgcctgc
      781 acccaggact cgccccgcgc gcggtagaag gagatccagg cgaagccgat ctgctgcagg
      841 gcggccgcga cggccaggcc ctggatcagg atcacggcgg gctcccactc ggcgcccagc
      901 acgtggtcga ccaggtcggc ggcgaacagc gccaggccgg cgcagaacgg cagcgcccag
      961 atcatggtca cccggctgga cttcacgaac agctcggcct ggatcgccag gtcgtcgacc
     1021 acctcgcaga tggcggggta gatcgtggtg gtcacgatct ggtccgcgcg gtcggcgtag
     1081 cgcgtcagcg tgaccgcgac ggtgatgaag ccggcggcgg ccaggccccc cttcaggtcg
     1141 aaggcgaaga tctggccctg cagcacgatc agcgtggcca gggcggtgaa caggatcggc
     1201 caggagaagg ccaggtagcg gcgcatggcc tggcgctcga agcgcagcgc cagccggtag
     1261 ggcgagagct tcagcgccgc cgccacggcc acgaggttgc ccgcggcggg cccgatcacc
     1321 atggcccaca cgctcccggt caccgccgcc agcggcaccg tgaccaggaa cgtcacggcg
     1381 gggatcgaga actgcagcag ccgcagccgc aggaagtcca tccggcggaa gaagatccac
     1441 tggggcgcct gcagcgcgaa cgcgatcggc aggtaggaca cggccagggt cagggccagc
     1501 agccgcatgt cgccgtagac cagcgcgacg accggcgcca gcagggccag caggaacgag
     1561 aacgccaccc cgatcgccag ctccagcgag aacgcgcgct ggaactcctc ctcctggccc
     1621 tcctcgtcct gctgcacgaa cgcctcgtcg atgcccacgc ggcgcagctg gaccatcgtc
     1681 atggccgtgg tggtcacgat cccgtacagg ccgatcgcca ccgggcccag cagcgccgtg
     1741 accaccagcc cctgcaccag cgccagcgcc tcgccgccgc ccaggaacag cgcgttcagc
     1801 accccgccgc ggatcgtccg cccgcgcagc tcctcgcgcg ggaagtgcag ccgctccgag
     1861 cgcccgctca ccagcgccgc ccaatgccgg ccgcgaaccc ctcggccagc ccgcgcagct
     1921 tcgctccggc catccgcgcg cgcagccgcg agcggcggcc cagcgcgctc ttggtcatgt
     1981 ccaggacgtc caccagcccc cagccccaca gccagagggc ggtcgccggg tggccggtgc
     2041 gcgcctgctt ggcggcgaag cccgcccagg cgcgcgagta gccgcggttg aggtccacca
     2101 gctccgccgg gctgcgccac tgacggtgcg tcgcgcgcac ctccggcgtc agcacggcct
     2161 tcgcgccgct gcgcagcagg cggtggttga agtccatgtc gtcggccgcg gggaacggct
     2221 ggacccccgg ccccaggcgc tcgtcccacc cacccagcgc gcgcgccgtg gcacagcgca
     2281 ccacgaacat cacgccgaag ccgatgctgc ccggcagcac ccagcgcccc tggcgcaccg
     2341 ccggccgggg caccgggaag gtggtcacca gcggcagccc gccgtcctcg cggtcgccgc
     2401 cgcccacgtg ccccgacagc cagtccgcgt cgccggcggc ccgcagctgg gcctgcagcg
     2461 ccgcgccgaa gccgggcgcc acggtgcagt cgtcgtccac gaacgcgatc caccggctct
     2521 ccacggcggc cagcgccacg ttgcgggcgc gcgacaggcc gcgccctggg tcgcggatga
     2581 cccgcacgtc gtcgcgacgg gccagggccg ggtcctcggc ggggctctgg tccaccacca
     2641 gcacctcgac ctgttccgag gcctcgcgcg tcagcgcgtc cagcgtctcg gccagctcgc
     2701 ccgggcggtc ccgggtgcac accgcgaccg tgaccagagt cgacgtc
//
LOCUS       DASUPW010000579         3788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_134470, whole genome shotgun sequence.
ACCESSION   DASUPW010000579 DASUPW010000000
VERSION     DASUPW010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134470"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VFY44_13085"
     CDS             complement(<1..569)
                     /locus_tag="VFY44_13085"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5911421.1"
                     /translation="MPSENALQGTVVLVTGASSGIGEASARDLAAHGAAVAIAARRAD
                     RLEKLAGEIEEAGGRALAIEADVTDRAQAQAAVQRTVDELGRLDTVVNNAGVMLLGPV
                     EGAPVDEWDAMVDLNVKGLMYVADAALPHLLKAAEDEPRQVADLVNISSVAGRVPRSG
                     SGVYNATKFGVGAFSESLRQEVTDRHVRVS"
     gene            complement(760..1185)
                     /locus_tag="VFY44_13090"
     CDS             complement(760..1185)
                     /locus_tag="VFY44_13090"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX5911422.1"
                     /translation="MSSPVLTGHCGCGAVTFEISEPLEGAVYCHCTRCQRRSGTAVQA
                     SAKVAPGSLTVTAGEEALKAWSPGGGYDKVFCGECGSALFARDPGTGELAIVRLGAID
                     GDPGIRPQAHQFVAYAAAWEAIPDDGLPRFDERAGEPPG"
     gene            complement(1350..1475)
                     /locus_tag="VFY44_13095"
     CDS             complement(1350..1475)
                     /locus_tag="VFY44_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911423.1"
                     /translation="MPEVAFPLAHHAAVSALPFFGPMLLIVAFLVVSRLRSSNSS"
     gene            complement(1468..3312)
                     /locus_tag="VFY44_13100"
     CDS             complement(1468..3312)
                     /locus_tag="VFY44_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911424.1"
                     /translation="MTRESTLSLATTLIAQGTEPAGGAATGEIMIATGAAMVVTVALL
                     VLGMGHRSGRVGLLDFAGDVGKRITGLPGWVAFPSGLATVSLITAVIGMYWDIALHIG
                     VGRDEGPLANPAHYFILAGLFGIFAAGFFAIVMPKEKPGPTAIRIGKDWDAPLGGVLI
                     CACGAFSLIGFPLDDVWHRLFGQDVTLWGPTHLMLIGGAAMTLVGIAVLLVEGARNVG
                     ARRLPLFLTVPLCGGLLLGLATFQAEFDFGVPQFRFVFGPILIMVAAGVGLVAARVFA
                     GRGAALGAVAFFLLVRGALALLVGPILGETTPHMPLFVVSALVVEALAFRVLPSEKPL
                     AFGLWAGVGIGTVGLASEWVWSHIWMPLPWPSELFPEAALLGFAAAVSGALLGAWAGS
                     RLAYTPAPGLPSLRRAAVVGAAGVAALVAFALYKPADEGVTARVALTDVRGGAERTVN
                     ADVRLSPVDAADNAEWLTATAWQGDGLVVDHLRKVGPGHYRTTEPIPVNGNWKSLIRL
                     HNGNSLTAVPVFLPRDQAIPAKEVPARAEFTRTFVADHQILQREQKAAAPGLTVAAYL
                     VVVAIALALLALLVWGLHRLAAAGPGATPPRAPREAAPRKPKLAGTHA"
     gene            3381..>3788
                     /locus_tag="VFY44_13105"
     CDS             3381..>3788
                     /locus_tag="VFY44_13105"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX5911425.1"
                     /translation="MASDTQPGMTVEQLAQASGMSVRNIRNHQSRGLLPPPEVRARTG
                     YYGPAHVERLRLIQQMQAEGFNLGAIQRLIGDEGPGADRFAAIRRLIADPDASEQPEV
                     LRADELTDRFGPIGPKVLARAQKLGVVVNLGDGT"
BASE COUNT          557 a         1378 c         1390 g          463 t
ORIGIN      
        1 gacacgcgca cgtggcggtc ggtgacctcc tggcgcaacg actcactgaa cgcgcccacg
       61 ccgaacttcg tcgcgttgta gacgccgctg ccggagcgtg gcacccggcc ggccaccgag
      121 ctgatgttca ccaggtcggc cacctgccgc ggctcgtcct cggctgcctt cagcaggtgg
      181 ggcagcgcgg cgtcggccac gtacatcagg cccttgacgt tcaggtcgac catggcgtcc
      241 cactcgtcca ccggagcgcc ctcgaccggg cccagcagca tcacgcccgc gttgttgacc
      301 acggtgtcca gccggcccag ctcgtcgacg gtgcgctgca cggcggcctg ggcctgcgcg
      361 cggtcggtca cgtcggcctc gatggccagc gccctgccgc cggcctcctc gatctcgccc
      421 gccagcttct ccagccggtc ggcgcggcgg gcggcgatgg ccaccgccgc cccgtgggcc
      481 gccaggtcgc gcgcgctggc ctctccgatt ccgctggagg ccccggtgac caggaccacg
      541 gttccctgaa gtgcgttctc tgaaggcatg gcccgaccct actcaggggc cccggtccgc
      601 cccgcgcggg agggcgagaa ccgaggtcgt gattgtgccg gctatagacc ccacaatcac
      661 gacctcgttc acctggcggc ccggctaccc ggacccgcta cccagcgccg ttcccggccg
      721 agcggctggt gcggcatacg gagaccgggg cggccggcgc tagccgggcg gctcgcccgc
      781 ccgctcgtcg aagcgcggca gcccgtcgtc ggggatcgcc tcccacgcgg cggcgtaggc
      841 cacgaactgg tgcgcctgcg gcctgatgcc gggatcgccg tcgatggcgc ccaggcgcac
      901 gatcgccagc tcgccggtgc cggggtcgcg tgcgaacagg gcggatccgc actcgccgca
      961 gaacaccttg tcgtagccgc cgccggggct ccatgccttc agcgcctcct cgccggcggt
     1021 cacggtcagc gagccggggg cgaccttggc cgacgcctgg acggccgttc cgctgcggcg
     1081 ctggcagcgc gtgcagtggc agtagacggc cccctcgagc ggctcgctga tctcgaaggt
     1141 aacggcaccg cagccgcagt gtccggtcag gacgggtgag ctcatggggg cgagactagc
     1201 cccgggcaag acaccggccc cgcggcgagg atcacatacc cggtctcttg cctcgtggcg
     1261 gttaagccgt tggcgtttgg cggcccgggt ggggtctctg gccgcgaggc cagtctgtgc
     1321 cagaccatac tcccttgtgg cgatcgttgc taggaagaat tcgagctgcg cagccgcgag
     1381 accaccagga aggcgacgat cagcagcatc ggcccgaaga agggcagggc cgacaccgcc
     1441 gcgtggtggg ccagcgggaa cgccacctca ggcatgggtg cccgccagct tcggcttgcg
     1501 cggcgcggcc tcgcgcggcg cgcgcggggg cgtggccccg ggcccggccg cggccagccg
     1561 gtgcaggccc cagaccagca gggccagcaa cgccagcgcg atggccacga ccaccaggta
     1621 ggcggccacg gtcaggccgg gggccgccgc cttctgctcg cgctggagga tctggtgatc
     1681 ggccacgaac gtccgcgtga actccgcgcg tgccgggacc tccttggccg ggatggcctg
     1741 gtcgcgcggc aggaacaccg gcaccgcggt cagcgagttg ccgttgtgca ggcggatcag
     1801 cgacttccag ttcccgttca ccgggatcgg ctcggtggtg cggtagtggc cggggccgac
     1861 cttgcgcagg tggtccacca ccaggccgtc gccctgccag gcggtggccg tcagccactc
     1921 ggcgttgtcg gcggcgtcca ccgggctcag gcgcacgtcc gcgttcacgg tgcgctcggc
     1981 gccgccgcgc acgtcggtca gcgcgacccg ggcggtcacg ccctcgtccg cgggcttgta
     2041 cagcgcgaac gccaccaggg cggccacccc ggcggcgccc accacggcgg cgcgccgcag
     2101 cgagggcagg ccgggggcgg gcgtgtaggc caggcgcgag cccgcccagg cgcccagcag
     2161 cgcgccggac acggcggcgg cgaagcccag cagcgcggcc tcggggaaca gctcggacgg
     2221 ccacggcagc ggcatccaga tgtgcgacca gacccactcg gaggccaggc cgacggtgcc
     2281 gatgcccacg ccggcccaca ggccgaaggc cagcggcttc tcgctgggca gcacgcggaa
     2341 ggcgagtgcc tcgaccacca gggccgagac cacgaacagc ggcatgtggg gcgtggtctc
     2401 gcccaggatg gggccgacca ggagcgccag ggcgccgcgc accagcagga agaaggcgac
     2461 cgcgcccagc gccgcgccgc ggccggcgaa cacgcgcgcg gccaccaggc cgacgccggc
     2521 ggcgaccatg atcaggatcg ggccgaacac gaaccggaac tgcggcacgc cgaagtcgaa
     2581 ctcggcctgg aaggtggcca ggcccagcag caggccgccg cacagcggca cggtcaggaa
     2641 cagcgggagc cggcgggcgc ccacgttgcg cgcaccctcc acgagcagca cggcgatgcc
     2701 gaccagcgtc atcgcggcgc cgccgatgag catcaggtgc gtcgggcccc agagcgtcac
     2761 gtcctgaccg aacaggcggt gccacacgtc gtccagcggg aagccgatca ggctgaaggc
     2821 gccacaggcg cagatcagga cgccgcccag cggcgcgtcc cagtccttgc cgatacggat
     2881 cgcggtcggg ccgggcttct ccttgggcat gacgatggcg aagaagccgg cggcgaagat
     2941 gccgaagagg ccggccagga tgaagtagtg cgcggggttg gccagcgggc cctcgtcgcg
     3001 gccgacgccg atgtgcagcg cgatgtccca gtacatgccg atcaccgcgg tgatcagcga
     3061 cacggtggcc agcccggagg ggaaggcgac ccagcccggc aggccggtga tgcgcttgcc
     3121 gacgtcgccg gcgaagtcca gcaggccgac gcggccggag cggtggccca tccccaggac
     3181 cagcagggcc acggtcacca ccatggcggc gccggtggcg atcatgatct cgccggtggc
     3241 ggcgccgccg gcgggctcgg tcccctgtgc gatcagtgtg gtggccaggc tcaaggtgct
     3301 ctccctcgtc atcggttgac gattacttca tacaacgtta cctcgattga ggttacatac
     3361 gatcggtagc gtttccaacc gtggcgtcgg acacacagcc cgggatgacg gtcgagcagc
     3421 tggcgcaggc gtcgggcatg tccgtgcgca acatccgcaa ccaccagtcg cgcggcctgc
     3481 tgcccccgcc cgaggtgcgc gcgcggacgg gctactacgg gcccgcgcac gtggagcgcc
     3541 tgcgcctgat ccagcagatg caggccgagg gcttcaacct gggggcgatc cagcggctga
     3601 tcggcgacga gggcccgggc gccgaccggt tcgccgccat ccgccggctc atcgccgacc
     3661 cggacgcgag tgagcagccc gaggtcctca gggcggacga gctgaccgac cggttcgggc
     3721 cgatcggccc gaaggtgctg gcccgcgccc agaagctggg cgtggtggtc aacctgggcg
     3781 acgggacg
//
LOCUS       DASUPW010000580         3501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1797775, whole genome shotgun sequence.
ACCESSION   DASUPW010000580 DASUPW010000000
VERSION     DASUPW010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1797775"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VFY44_13110"
     CDS             <1..369
                     /locus_tag="VFY44_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911426.1"
                     /translation="ERQRLALEVVLGGGPCAVVCLDEPTRGMDRVRKDALAARLELLA
                     AGGAAVLVATHDTEFAAHFARRVVLMGQGDLIADGTAEEVLAGGWHYSTETARVLDGA
                     GGALTPEQGGRVLAGRLVAR"
     gene            366..1013
                     /locus_tag="VFY44_13115"
     CDS             366..1013
                     /locus_tag="VFY44_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911427.1"
                     /translation="MSAHVSAYAALGLILAVAFGWYEMRRPPARTVALVAAMAALAVV
                     GRLAFAAVPGVKPTTDIVLFAGYALGGVPGFMVGAVTAVASNVFFGQGPWTVWQMVAW
                     GAVGAGGAGLARLQRGREPSRWTLAAACAVAAVAFGAFMDLYQWSFAAEQSAGAYGAV
                     AGTSLAYNVAHVAASVAFALLAGPTFVRAIRRYQRRFEVQWDPPPDPGPLSAPVR"
     gene            1024..1536
                     /locus_tag="VFY44_13120"
     CDS             1024..1536
                     /locus_tag="VFY44_13120"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEX5911428.1"
                     /translation="MELEEAIRGRRTHKVYGSDPVDRDTLEELLELGRWAPNHHLTEP
                     WEFRVVGPQTLAALKEAAGPEAAAKLDRAPTLVAVSVVQSGSDPVQDEEDLLAAGCAV
                     YAVLLGAHGRGLVGYWRTPGFLREPAGAAALGMRPDEHFVALIHLGRQRQDQRPPERS
                     PVSEYVSFLP"
     gene            1533..3215
                     /locus_tag="VFY44_13125"
     CDS             1533..3215
                     /locus_tag="VFY44_13125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEX5911429.1"
                     /translation="MIARADALAAMESDEFDVVVIGGGITGAGVALDAASRGYSVALV
                     ERNDYSSGTSSRSSKLVHGGLRYLQNFDLGLVREALVERQRMVNLAPHLVKPLPLLIP
                     AFDGKHPDRKSGVGLNLYDVMSRERRSSRRRGTATDPEYWSPERHRTVDAAETLEMLP
                     ALAPREPTSAYLFYDCQTDDTRLVFTVLGEAERFGAVCANAVEVEGLVERGGRASGVL
                     CRDALGGGEFELAAEHVVNATGVWADRIRPDELRDEAEVPRIKPSRGTHVTVSLDKLP
                     VVSGAIVPSGGGRFIFVLPWLGRTLIGTTDVTYESDQLDHIPAAAEDVEYLLDAVNEF
                     FATDLTAADLSGAYAGVRPLISTGDPKKAVDISRKDELYETSSGMITITGGKLTTWRH
                     MAQRTVDRIVEREGREAPCRTTEIPLGMPLAPGELDGHPQLDDAQRAHLAGRYGHAAV
                     DVMRMAEEGGPELAERIVPELPDLLVEAPFAARREQARSLEDALLRRTRLGLLDARTL
                     CAPGSPATRRVADAMAAELGWDGARVEAELEQWARVAALEGLVPAEPVPEPA"
     gene            3212..>3501
                     /locus_tag="VFY44_13130"
     CDS             3212..>3501
                     /locus_tag="VFY44_13130"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX5911430.1"
                     /translation="MRLALRRRSVELAQGAPLVMGIVNATPDSFSDRQGAKDPGELAE
                     RAFALADQGAHIIDVGGESGRTDRAAVSEAEEAERIVPLVRRLADAGVAVSV"
BASE COUNT          413 a         1290 c         1356 g          442 t
ORIGIN      
        1 gagcgccagc gcctggcgct ggaggtggtc ctgggcggcg ggccgtgcgc ggtggtgtgc
       61 ctggacgagc cgacccgggg gatggaccgc gtgcgcaagg atgcgctcgc ggccagactc
      121 gagctgctgg cggcgggcgg cgccgcggtg ctggtggcca cccatgacac cgagttcgcc
      181 gcccacttcg cccggcgcgt cgtgctgatg ggccagggcg acctgatcgc cgacgggacc
      241 gccgaggagg tgctggccgg tggctggcac tactccaccg agaccgcccg cgtcctggac
      301 ggcgccggcg gcgccctgac ccccgagcag ggcggccgcg tgctggccgg ccggctggtg
      361 gcccggtgag cgcgcacgtg agcgcctacg ccgcgctggg gctgatcctg gcggtcgcct
      421 tcgggtggta cgagatgcgc cgcccgccgg cccgcaccgt cgcgctggtg gccgccatgg
      481 ccgccctggc cgtggtcggg cgcctggcct tcgccgccgt ccccggcgtg aagcccacca
      541 ccgacatcgt cctgttcgcc ggctacgcgc tggggggcgt gcccgggttc atggtcgggg
      601 ccgtgacggc ggtggcctcg aacgtgttct tcggccaggg gccctggacc gtctggcaga
      661 tggtggcgtg gggcgccgtg ggcgccggcg gggcgggcct ggcccggcta cagcgcgggc
      721 gcgagccgtc gcggtggacg ctggccgccg cctgcgcggt ggccgccgtg gcgttcgggg
      781 cgttcatgga cctctaccag tggagcttcg ccgctgagca gtccgcgggc gcctacggcg
      841 cggtggcggg cacctcgctg gcctacaacg tcgcccacgt ggcggccagc gtcgcgttcg
      901 cgctgctggc cggcccgacc ttcgtccgcg ccatccgccg ctaccagcgc cggttcgagg
      961 tgcagtggga cccgccgccc gaccccgggc cgctgtccgc gccggtccgc tagcgtcgca
     1021 gccgtggagc tggaagaggc gatccgcggg cggcgcaccc acaaggtcta cgggtccgat
     1081 cccgtggacc gggacacgct cgaggagctg ctggagctgg gccgctgggc ccccaaccac
     1141 catctgaccg agccgtggga gttccgcgtc gtggggccgc agacgctcgc cgccctgaag
     1201 gaggccgcgg gacccgaggc cgccgccaag ctcgaccgcg cgcccacgct ggtggccgtg
     1261 tcggtggtcc agagcggaag tgacccggtc caggacgagg aggacctgct ggccgccggc
     1321 tgcgcggtct acgccgtcct cctcggcgcc cacggccgcg ggctggtggg ctactggcgc
     1381 acgcccgggt tcctgcgcga gcccgcgggc gcggccgcgc tgggcatgcg cccggacgag
     1441 cacttcgtgg cgctgatcca cctgggccgc cagcgccagg accagcgccc gcccgagcgc
     1501 tccccggtga gcgagtacgt gagcttcctg ccgtgatcgc ccgcgccgac gccctggccg
     1561 ccatggagtc ggacgagttc gacgtggtgg tcatcggcgg cggcatcacc ggggcggggg
     1621 tggccctgga cgcggcgtcg cgcggctaca gcgtcgcgct ggtcgagcgc aacgactact
     1681 cgtcgggcac ctcgagccgg tcctcgaagc tggtccacgg cggcctgcgc tacctccaga
     1741 acttcgacct cgggctggtc cgcgaggcgc tggtcgagcg ccagcgcatg gtcaacctgg
     1801 cgccccacct ggtcaagccg ctgccgctgc tgatcccggc cttcgacggc aagcacccgg
     1861 accgcaagtc gggcgtgggg ctgaacctct acgacgtgat gagccgcgag cgccgcagct
     1921 cgcgccgccg cgggaccgcc accgaccccg aatactggag ccccgagcgc caccgcaccg
     1981 tggacgcggc cgagacgctg gagatgctgc ccgccctggc tccgcgcgag cccacctcgg
     2041 cgtacctgtt ctacgactgc cagaccgacg acacccggct ggtgttcacc gtgctcggcg
     2101 aggccgagcg cttcggcgcc gtgtgcgcca acgcggtcga ggtcgagggc ctggtcgagc
     2161 ggggcggccg cgcctccggg gtgctgtgcc gcgatgcgct gggcggcggc gagttcgagc
     2221 tggccgccga gcacgtggtc aacgccaccg gcgtgtgggc cgaccggatc cgccccgacg
     2281 agctgcgcga cgaggccgag gtgccgcgca tcaagcccag ccgcggcacc cacgtgaccg
     2341 tctcgctgga caagctgccc gtggtctcgg gcgcgatcgt gccgtcgggg ggagggcggt
     2401 tcatcttcgt gctgccgtgg ctgggccgga cgctcatcgg gaccaccgac gtgacctacg
     2461 agagcgacca gctcgaccac atccccgcgg ccgccgagga cgtcgagtac ctgctggacg
     2521 cggtcaacga gttcttcgcc accgacctga ccgcggccga cctgagcggc gcgtacgcgg
     2581 gcgtgcggcc gctgatctcc accggcgacc cgaagaaggc ggtggacatc tcgcgcaagg
     2641 acgagctgta cgagaccagc tcgggcatga tcacgatcac cggcggcaag ctcaccacgt
     2701 ggcggcacat ggcccagcgc acggtggacc ggatcgtcga gcgcgagggc cgcgaggcgc
     2761 cctgccggac cacggagatc ccgctcggca tgccgctggc gcccggcgag ctggacggcc
     2821 accctcagct ggacgacgcc cagcgcgccc acctggccgg ccgctacggg cacgcggcgg
     2881 tcgacgtgat gcggatggcc gaggagggtg ggcccgagct ggccgagcgg atcgtgcccg
     2941 agctgcccga cctgctggtc gaggcgccgt tcgccgcccg ccgcgagcag gcccggtcgc
     3001 tggaggacgc gctgctgcgc cgcacccgcc tgggactgct ggacgcgcgc accctgtgcg
     3061 cgccgggatc gccggccacc cgccgcgtgg ccgacgccat ggccgccgag ctgggctggg
     3121 acggcgcccg ggtggaggcc gagctggagc agtgggcgcg ggttgccgcc ctggagggcc
     3181 tggtgcccgc cgagcccgtc cccgagccgg catgaggctg gccctccgcc ggcggagcgt
     3241 cgagctggcg cagggggcgc cgctggtcat gggcatcgtc aacgcgaccc cggactcgtt
     3301 ctcggaccgc cagggcgcga aggacccggg cgagctggcc gagcgcgcgt tcgcgctggc
     3361 cgaccagggc gcccacatca tcgacgtggg cggcgagtcc ggccgcaccg accgggccgc
     3421 ggtgtccgag gccgaggagg ccgagcggat cgtgccgctg gtgcgccgcc tggccgacgc
     3481 cggcgtcgcc gtgtcggtgg a
//
LOCUS       DASUPW010000581         5354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_688429, whole genome shotgun sequence.
ACCESSION   DASUPW010000581 DASUPW010000000
VERSION     DASUPW010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688429"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VFY44_13135"
     CDS             <1..366
                     /locus_tag="VFY44_13135"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX5911431.1"
                     /translation="LKRWFHAGHDWDTTEATVDFRVGGTLRVVMHNPHEDEEYGGGGE
                     YTEIDPPRRLAFTWVWDDERDQYSQLVEVEFEEHDGRTKVLFSHHGLWDEQAVTDHEG
                     GWTIVLDNLERDLRKERGG"
     gene            complement(541..1947)
                     /locus_tag="VFY44_13140"
     CDS             complement(541..1947)
                     /locus_tag="VFY44_13140"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX5911432.1"
                     /translation="MRRVSYALVGVLVACLAFSATASAAPVTKTFRTGPIKVGSYQVK
                     QSDLDASIPTPGIDGYITQMDVEVVDRAGKPIPINRLMLHHIVFSNLGPSFGSKRDGT
                     CGSFTLLNSRSTVPGIAERFYAAGEERAKMRLPAGYGYKINAADKWGMTWMLMNHRKV
                     VDTAYIQYRVTYETAPMTPVKPYWLDIENCKADPVFDIPGGKAKGATTRRSSVWNVPE
                     SGRIVAGGGHVHGGAKQLDLKRTGPDCTLYSSKPTWGSAKHPFYNVKPVLHEPGPINM
                     SGFTSGAGMPVRQGERLRLDADYDGELMHTRVMGIFILFLAPDGAAPATKSCARPADL
                     RDWTTSERGRKKPPRFTVPLIGLDGGGKAREIEAPKGARRNLRSGARIGVGDSFFSQK
                     NVSVAAGSTLKWKFDGRLLHNVTVADGPRGFSSAHLDQGRTYKAKLSAPGTYKLFCAL
                     HPVQMTETIKVRSAKRKK"
     gene            complement(1975..2598)
                     /locus_tag="VFY44_13145"
     CDS             complement(1975..2598)
                     /locus_tag="VFY44_13145"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX5911433.1"
                     /translation="MITTVISDFGGVLTTPLGNAFAAWSQESGVPLEFLGEAMEKATH
                     AHDGQHPLFLLERGELTEREFLDRLQAAVPGGHDLEGMFDVWFGFLERNHEMIELMRD
                     LKARGLRMALLTNNVREWEPRWRPMLPELDEIFEVVVDSGFVGMRKPEPGIYELTLER
                     LGGVPASECLFVDDMELNCEAARELGMTAVQFHDTEQARREVEAVLA"
     gene            complement(2595..3299)
                     /locus_tag="VFY44_13150"
     CDS             complement(2595..3299)
                     /locus_tag="VFY44_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911434.1"
                     /translation="MSDALSLGLFDPEHGLNVSARAGATLLFEGGRADIRDQGPEIEP
                     TRKGWQARLGQDFDVKLEPVAEPAVLGPDVTVHVCQVSGRIGGQKARCLGTVAQTHSA
                     PAWDELDALRSVAAVFDTDNAFLAVARRPRGAVGHDAEEVSAWLLSEGEVLDLDQARL
                     STVYDGNGRQRSAGLELWVSGQDFPMRGSGTVVAGTSLALEGLEVHAAAFRWRMEDRE
                     GAGAYELWLRSEPEPA"
     gene            complement(3296..3760)
                     /locus_tag="VFY44_13155"
     CDS             complement(3296..3760)
                     /locus_tag="VFY44_13155"
                     /inference="COORDINATES: protein motif:HMM:NF016102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HEX5911435.1"
                     /translation="MPLLLLFVVFIVVPLAELYVILKVGDSIGWLPTIGLLALDSVLG
                     TVLLRSQGRTVWRRFQEALAAGRMPHREVQDGVAVIFGGAFLITPGFLTDILGFALLL
                     PPTRALIIRLSQRRLGRFMADRVTVRRATTPGADVEGTATEYPPTDPPRIER"
     gene            3795..4100
                     /locus_tag="VFY44_13160"
     CDS             3795..4100
                     /locus_tag="VFY44_13160"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEX5911436.1"
                     /translation="MAEAPTTDDVQEALENVIDPELGLDFVELGLVYDIAVEGSEVFI
                     TFTLTSPGCPIGPQVSEQMEEFVGDLEGVTAVYPKMVFTPPWTPELMSEDAKFALGF"
     gene            4159..5259
                     /locus_tag="VFY44_13165"
     CDS             4159..5259
                     /locus_tag="VFY44_13165"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5911437.1"
                     /translation="MTAIQGLHIEEERPLAGKLAGALYLTGSATGLSLFAVPGVDVTH
                     PAALLVVVALGIAWGVAALTVVPWDKAPPIVSHLSCFMGLPIAAVAMAATGGATSPTR
                     FYLLFIIFYASYFYLPREAAFYIVGCSIVHALPLFYESGATDAGFVGELLVIIPTYFV
                     LGALIIAAKRLQVEMRERADALALSDPLTGVANRRAFEAMMDLGRSGGRSSDRIGLLL
                     VDLDDFKAANTMFGHTGGDRVLCAAADALREAARGNDLVARLGGDEFAILATGISRPA
                     TERLAQRVLDKVAAADRELDLPGFRLSASVGWATHPDPVEDRAELYRNADTALGEVKS
                     GGKASWRSADPARSPTVVGDQTTSRNLPVSTS"
BASE COUNT          755 a         1918 c         1869 g          812 t
ORIGIN      
        1 ctcaagcgct ggtttcacgc cgggcacgac tgggacacca ccgaggccac cgtcgacttc
       61 cgcgtcggcg gcacgctgcg ggtggtcatg cacaacccgc acgaggacga ggagtacggc
      121 ggcgggggcg agtacaccga gatcgacccg ccgcggcggc tggccttcac gtgggtgtgg
      181 gacgacgagc gcgaccagta ctcccagctg gtggaggtcg agttcgagga gcacgacggg
      241 cgcacgaagg tgctgttcag ccaccacggg ctgtgggacg agcaggccgt gaccgaccac
      301 gagggcggct ggaccatcgt gctggacaac ctcgagcgcg atctccgcaa agagcggggt
      361 ggttgaggac ggacccgttg accgcccggc ggggtcgtgt ttcagttcct cgcagagaca
      421 cggacccagg cgcaaagagc gaggccacga ggtgtgaggc ccgccgcgca gccggtcgga
      481 cttcggtccg gcttggcgcg cacctcgagc gccgggaccg tgtctcgtct acgttccggg
      541 ctacttcttg cgcttggccg agcggacctt gatggtctcg gtcatctgca ccgggtgcag
      601 cgcgcagaac agcttgtagg tgcccggggc gctgagcttg gccttgtacg tgcggccctg
      661 gtccaggtgg gccgacgaga agccgcgcgg cccgtcggcc acggtcacgt tgtgcagcag
      721 ccggccgtcg aacttccact tcagcgtcga gccggcggcc accgacacgt tcttctggct
      781 gaagaagctg tcacccacgc cgatgcgcgc cccgctgcgc aggttgcggc gcgcgccctt
      841 cggcgcctcg atctcgcgcg ccttgccgcc cccgtccagg ccgatcagcg gcacggtgaa
      901 gcgcgggggc ttcttgcggc cgcgctcgct ggtggtccag tcgcgcaggt cggcgggccg
      961 ggcacacgac ttcgtggccg gggccgcccc gtcgggggcc aggaacagga tgaagatccc
     1021 catcacgcgg gtgtgcatca gctcgccgtc gtagtcggcg tccaggcgca atcgctcgcc
     1081 ctggcggacg ggcatgcccg caccgctggt gaagccgctc atgttgatcg gcccgggctc
     1141 gtgcagcacc ggcttgacgt tgtagaaggg gtgcttggcc gagccccacg tgggcttgga
     1201 gctgtacagc gtgcagtcgg gcccggtgcg cttgaggtcg agctgcttgg cgcccccgtg
     1261 cacgtggccg cctccggcca cgatccggcc cgactcgggc acgttccaca cgctcgaccg
     1321 gcgcgtggtg gcgcccttgg ccttgccgcc ggggatgtcg aacaccgggt ccgccttgca
     1381 gttctcgatg tccagccagt agggcttcac cggcgtcatg ggcgccgtct cgtaggtcac
     1441 ccggtactgg atgtaggcgg tgtccaccac cttgcggtgg ttcatgagca tccaggtcat
     1501 gccccacttg tccgcggcgt tgatcttgta gccgtagccc gcgggcaggc gcatcttcgc
     1561 ccgctcctcg ccggccgcgt agaagcgctc ggcgatgccc gggacggtgc tgcgtgagtt
     1621 cagcaacgtg aagctgccgc acgtgccgtc gcgcttggag ccgaacgagg ggcccaggtt
     1681 gctgaacacg atgtggtgca gcatcaggcg gttgatcggg atcggcttgc cggcgcggtc
     1741 caccacctcg acgtccatct gggtgatgta gccgtcgatg cccggggtgg ggatcgaggc
     1801 atccaggtcg ctctgcttga cctggtagga gccgaccttg atcgggccgg tgcggaaggt
     1861 cttggtgacc ggcgccgccg acgccgtggc gctgaaggcc aggcaggcca cgaggacgcc
     1921 gacgagggcg tagctcactc tcctcacggg cccagggtag gccacgcaag cgggctacgc
     1981 cagtaccgct tcgacctcgc gccgcgcctg ctcggtgtcg tggaactgca ccgcggtcat
     2041 gcccagctcg cgcgcggcct cgcagttcag ctccatgtcg tccacgaaca ggcactccga
     2101 cgcgggcacg ccccccagcc gctccagggt cagctcgtag atgccgggct cgggcttgcg
     2161 catgcccacg aacccggagt ccaccaccac ctcgaagatc tcgtccagct ccggcagcat
     2221 cggccgccag cgcggctccc actcgcggac gttgttggtc agcagcgcca tccgcaggcc
     2281 gcgcgccttc agatcgcgca tcagctcgat catctcgtgg ttgcgctcca ggaagccgaa
     2341 ccagacgtcg aacatgccct ccaggtcgtg gccgcccggc accgccgcct ggagccggtc
     2401 caggaactcg cgctcggtca gctcgccccg ctccagcagg aacagcgggt gctggccgtc
     2461 gtgggcgtgg gtggccttct ccatcgcctc gcccaggaac tccagcggca cgccggactc
     2521 ctgcgaccag gcggcgaagg cgttgcccag cggcgtggtc agcacgccgc cgaagtcgga
     2581 gatcacggtg gtgatcacgc cggctcgggc tcgctgcgca gccacagctc gtacgcgccg
     2641 gcgccctcgc gatcctccat gcgccagcgg aaggcggcgg cgtgcacctc caggccctcc
     2701 agcgccagcg acgtgcccgc gaccacggtg cccgagcccc gcatggggaa gtcctggccg
     2761 gagacccaca actccaggcc ggcgctgcgc tggcgcccgt tgccgtcgta gacggtggac
     2821 aggcgggcct ggtccaggtc cagcacctcg ccctcggaga gcagccacgc cgacacctcc
     2881 tcggcgtcgt ggcccacggc gccgcgcggg cgccgtgcca ccgccaggaa ggcgttgtcg
     2941 gtgtcgaaca ccgccgcgac cgagcgcagc gcgtccagct cgtcccacgc gggcgcgctg
     3001 tgggtctggg ccaccgtccc caggcagcgc gccttctggc cgccgatccg gccgctgacc
     3061 tggcacacgt gcacggtcac gtcgggcccc agcaccgccg gctcggccac cggctcgagc
     3121 ttgacgtcga agtcctggcc cagccgggcc tgccacccct tgcgcgtcgg ctcgatctcg
     3181 gggccctggt cgcggatgtc cgcgcggccg ccctcgaaca gcagcgtcgc gcccgcgcgg
     3241 gcgctgacgt tgaggccgtg ttcgggatcg aacaggccga gcgagagggc gtcgctcacc
     3301 gttcgatccg tggcggatcg gtcggcgggt actcggtggc ggtgccctcc acgtcggcgc
     3361 ccggggtggt cgcccgccgg accgtcacgc ggtcggccat gaaccggccc agccggcgtt
     3421 gcgacaggcg gatgatcagc gcgcgcgtgg ggggcagcag cagggcgaag cccaggatgt
     3481 cggtcaggaa gccgggcgtg atcaggaacg ccccgccgaa gatcacggcg accccgtcct
     3541 ggacctcccg gtgtggcatc cggccggccg ccagggcctc ctggaagcga cgccacacgg
     3601 tccgcccctg ggagcgcaac agcacggtgc ccagcaccga atccagggcc agcaggccga
     3661 tggtcggcag ccacccgatc gagtccccga ccttgaggat cacgtacagc tcggccagcg
     3721 gcacgacgat gaagaccacg aagaggagca gcagcggcat ggatttcagc ctatcggcct
     3781 ctaagcttgc ccccatggcc gaggcaccga ccaccgacga cgtccaggaa gccctggaga
     3841 acgtgatcga tcccgagctc gggctggact tcgtggagct cggcctggtg tacgacatcg
     3901 ccgtcgaggg cagcgaggtg ttcatcacct tcacgctgac ctcgccgggc tgcccgatcg
     3961 ggccgcaggt gtccgagcag atggaggagt tcgtcggtga cctcgagggc gtcacggccg
     4021 tctatccgaa gatggtcttc accccgccct ggacgccgga gctgatgtcc gaagacgcga
     4081 agttcgcgct cggcttctag acgcctgtcc cattacgctc aaggtctgcc gatttcggga
     4141 cgatgggttc ccggggacgt gactgcaata caggggcttc acatagagga ggagcgaccg
     4201 ctggcgggca agctggccgg cgctctgtac ctgaccggct cggcgacggg cctgtcgctg
     4261 ttcgcggtgc ccggggtcga cgtgacgcat ccggcggccc tcctggtggt cgtcgcgctg
     4321 gggatcgcct ggggcgtcgc cgcgctgacg gtcgtgccgt gggacaaggc gcccccgatc
     4381 gtctcgcacc tgtcgtgctt catgggcctg ccgatcgcgg ccgtggccat ggccgccacg
     4441 ggcggcgcca cctcgcccac ccgcttctac ctgctcttca tcatcttcta cgcctcgtac
     4501 ttctacctcc cgcgcgaggc cgcgttctac atcgtcgggt gctcgatcgt gcacgcgctt
     4561 cccctgttct acgagtcggg ggccaccgat gccgggttcg tgggcgagct cctggtgatc
     4621 atccccacct acttcgtgct cggcgcgctg atcatcgccg ccaagcgcct gcaggtcgag
     4681 atgcgcgagc gcgcggacgc cctggccctg tccgacccgc tgacgggcgt cgccaaccgg
     4741 cgcgccttcg aggccatgat ggacctgggc cgctcgggcg ggcgctccag cgaccggatc
     4801 ggcctgctgc tggtggacct ggacgacttc aaggcggcca acacgatgtt cggccacacc
     4861 gggggcgacc gcgtcctgtg cgccgccgcc gatgcgctcc gcgaggccgc ccgcggcaac
     4921 gacctcgtcg cccggctggg cggggacgag ttcgcgatcc tggccaccgg catcagccgc
     4981 ccggccaccg agcgcctggc ccagcgggtg ctggacaagg tcgccgcggc cgaccgcgag
     5041 ctcgacctgc ccggcttccg gctctcggcc agcgtggggt gggccaccca ccccgatccc
     5101 gtcgaggacc gcgccgagct ctaccgcaac gcggacacgg ccctgggtga ggtcaagtcc
     5161 ggtggcaagg cgtcctggcg ctccgccgac ccggcacggt cgccgacggt cgtcggcgat
     5221 cagacgacct cgcgcaacct gcccgtctcg acctcgtaga cgaagccccg caccgagtcg
     5281 gtgcggggga tgaacgggct ctccacgacc tgcctgaccg agtcgcggac gctggcctcc
     5341 agatcctcga acgc
//
LOCUS       DASUPW010000582         4142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_550857, whole genome shotgun sequence.
ACCESSION   DASUPW010000582 DASUPW010000000
VERSION     DASUPW010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550857"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="VFY44_13170"
     CDS             complement(<1..949)
                     /locus_tag="VFY44_13170"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX5911438.1"
                     /translation="MADAVSIREVGPRDGFQNEPEVIPTAAKIALIDQLARTGLRRLE
                     VTSFVRADVIPQLADAAEVLPAIDVPDEVEVAVLVPNERGLDNALGLREHFHQANLFL
                     SATESHNRANVNRSVQESLAGLERVTERSLAEDLRVAAVISVAFGCPYEGPVEPETVF
                     AIADRLVAAGVAEIGFGDTTGMANPAQVREFFGLAFERLPDVELTAHFHNTRGQGLAN
                     ALAALEAGVRSFESSFGELGGCPVPAGATGNVASEDLVSMLHEMGHDTGIDLEALLAC
                     ARAVQEELGRPLGSHLLTAGPVDWAGGAARTSRPAENDRL"
     gene            1132..2619
                     /locus_tag="VFY44_13175"
     CDS             1132..2619
                     /locus_tag="VFY44_13175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5911439.1"
                     /translation="MSDHRKRLVLVACILGSTVVAVDSTVVNVALPAIREDLGGGFAG
                     QQWTANAYLITLASLILVGGSLGDVLGERRVFTAGLVGFGVTSLLCAVAPTIEVLVLA
                     RALQGVSGALLSPAALAVIVMTFPRDERGKAVGSWTAWGASGVVIGPLIGGQLVDAAS
                     WRWIFALNVPLVLGTLALVARVVPEGVRGDDHPPIDVGGALQCAAGLAALTFGLVRAP
                     EVGLADPTVLVPLAAALVLLVAFVLHERRSEHPMLNLELFRIHNFAIGNLQTLSMYAG
                     LSLLFFFLVIFLQAGAGYSAVAAGTASVPVTLVMFGLSSRFGALADRHGPRFFMGVGP
                     LVAATGLALMTTVDTDVSYFTELLPPLLVFSLGLSMTVAPLTATVLAAADEGNAGTAS
                     GINNAIARTAGLLGIALVGALVAGRYGAEADSSADAFHFAMVISAVLVALGGLAALIG
                     IQNPTREVEAEGCPGGQLAGTPRETARVSGGVGAPGDAVPAEPAS"
     gene            complement(2564..3091)
                     /locus_tag="VFY44_13180"
     CDS             complement(2564..3091)
                     /locus_tag="VFY44_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911440.1"
                     /translation="MATVVQLWRRLGFEQKVAAVASVLLIVSTFGPFSLVEAGVILTA
                     LGVLALLRARAEGRHFHLPFGDGTVIMAAAGWAALLIVTRLFDRPLGQNVLALACAAL
                     LAAAGLRERAIRPRDDVPSDPMPPPDPVVAREAPEDDPVTVPLARDEDATAALPPTTR
                     RAPRGPRPQGPPRPR"
     gene            complement(3160..>4142)
                     /locus_tag="VFY44_13185"
     CDS             complement(3160..>4142)
                     /locus_tag="VFY44_13185"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX5911441.1"
                     /translation="YGHGTAMSGGAAAAANTIGVTGVAPDAKIMPVKVLADDGSGNTS
                     SVITGLRYAIANGADVINLSMNGPDRSQALEETLAAAKAAGIVVVASAGNDGANRDAS
                     PSYPASAPGLTLPVAAQSQDGGLATFSAYGRSVLLSAPGQDVLSTARGGAYASSSGTS
                     IATAQVSGAAALLAAARPTATPDQIRDALVGGVRHMSIDAQMVGSGGSLDASRAMTRL
                     IPGAGPRVTLASRRLIRSKTGRIALKWRARGAVGAVAQYRVKVGRRSFAVHASSVAKM
                     ASAQRRTMRLKAGRYRFTVAAYDASGRPLATRAGKVKVARKKHRRASKRS"
BASE COUNT          494 a         1457 c         1620 g          571 t
ORIGIN      
        1 ccagccggtc gttctcggct ggtctgctgg tgcgggccgc gccgcccgcc cagtccaccg
       61 ggcccgcggt cagcaggtgc gagcccagcg ggcgccccag ctcctcctgg acggcccggg
      121 cgcaggccag cagcgcctcg aggtcgatgc ccgtgtcgtg gcccatctcg tgcagcatcg
      181 agaccaggtc ctcgctggcc acgttgccgg tggccccggc cgggaccggg cagccgccca
      241 gctcgccgaa ggaggactcg aacgagcgca cgccggcctc cagggcggcc agcgcgttgg
      301 ccaggccctg cccgcgggtg ttgtggaagt gggcggtcag ctcgacgtcc ggcaggcgct
      361 cgaaggcgag gccgaagaac tcgcgcacct gggccgggtt ggccatgccg gtggtgtcgc
      421 cgaagccgat ctcggccacg cccgcggcca ccagccggtc ggcgatcgcg aacacggtct
      481 cgggctcgac cgggccctcg taggggcagc cgaaggccac cgagatcacc gcggccacgc
      541 ggaggtcctc ggccagcgac cgctcggtca cgcgctccag gccggccagc gactcctgga
      601 ccgaccggtt cacgttggcg cggttgtgcg actcggtggc cgacaggaac aggttggcct
      661 ggtggaagtg ctcgcggagc ccgagcgcgt tgtccagccc gcgctcgttg ggcaccagca
      721 cggccacctc gacctcgtcc ggcacgtcga tggcggggag cacctcggcg gcgtcggcca
      781 gctgggggat cacgtccgcc cggacgaagc tggtgacctc gagccggcgc aacccggtgc
      841 gcgccagctg gtcgatcagc gcgatcttgg ccgcggtggg gatgacctcg ggctcgttct
      901 ggaacccgtc gcgcgggccg acctcgcgga tggagacggc gtcggccatg ccctgaggat
      961 atggccccgg aaagcgaaac ggccggcccc gattggggac cggccgcctc gttccgacag
     1021 gagggaggtt gagagagacg ccggtaccgg aaaatccgtt acgagtgccg ctctatgcag
     1081 gcatatcggc ggtaccgcca agaaccttga gccccgcggc atactcgggt ggtgagcgac
     1141 caccgcaagc gcctggtcct ggtggcctgc atcctcggct cgaccgtggt ggcggtggac
     1201 tcgacggtgg tcaacgtggc gctgccggcc atccgcgagg acctgggcgg tggcttcgcc
     1261 ggccagcagt ggacggccaa cgcctacctg atcacgctgg cctcgctgat cctggtcggt
     1321 ggctcgctgg gcgacgtcct gggcgagcgg cgggtgttca cggccgggct ggtcggcttc
     1381 ggcgtgacgt cgctgctgtg cgcggtcgcg cccacgatcg aggtcctggt gctggcacgg
     1441 gcgctgcagg gcgtcagcgg ggcgctgctg tccccggcgg cgctggccgt gatcgtcatg
     1501 accttcccgc gcgacgaacg cggcaaggcc gtcggctcat ggacggcctg gggcgccagc
     1561 ggcgtggtca tcgggccgct gatcggcggg cagctggtcg acgcggcctc gtggcgctgg
     1621 atcttcgcgt tgaacgtgcc gctggtgctg ggcacgctgg cgctggtggc ccgcgtggtg
     1681 ccggagggcg tccgcggcga cgaccacccg ccgatcgacg tgggcggtgc gctgcagtgc
     1741 gcggccgggc tggcggccct gaccttcggc ctggtgcgcg cccccgaggt cggcctggcc
     1801 gaccccaccg tcctggtccc gctggcggcg gcgctggtgc tgctggtggc cttcgtcctg
     1861 cacgagcgcc ggtccgagca cccgatgctg aacctggagc tgttccggat ccacaacttc
     1921 gccatcggca acctgcagac gctgtccatg tacgccggcc tgtcgctgct gttcttcttc
     1981 ctggtgatct tcctgcaggc gggagcgggc tacagcgcgg tggcggcggg caccgcgtcg
     2041 gtgccggtca cgctggtcat gttcggcctg tcgtcgcgct tcggggccct ggccgaccgc
     2101 cacgggccgc gcttcttcat gggcgtcggg ccgctggtgg cggccacggg gctcgcgctg
     2161 atgaccaccg tggacaccga cgtcagctac ttcaccgagc tgctgccgcc gctgctggtg
     2221 ttctcgctcg gcctgtcgat gaccgtcgcc ccgctgaccg ccacggtgct ggccgccgcc
     2281 gacgagggca acgccgggac cgccagcggc atcaacaacg ccatcgcgcg cacggcgggg
     2341 ctgctgggca tcgcgctcgt gggcgcgctg gtggcgggcc gctacggcgc cgaggccgac
     2401 agctccgccg acgcgttcca tttcgccatg gtcatctcgg cggtgctggt ggccctgggc
     2461 gggctcgccg ccctgatcgg catccagaac cccacccgcg aggtcgaggc cgagggctgc
     2521 cccggcgggc agctggccgg cacgccgcgt gagacggcgc gggtcagcgg gggcgtgggg
     2581 gcccctgggg acgcggtccc cgcggagccc gcctcgtagt cggcggcagc gccgcggtgg
     2641 cgtcctcgtc gcgggccagg ggcaccgtga cgggatcgtc ctccggcgcc tcccgggcaa
     2701 cgaccgggtc cggcggcggc atggggtccg agggcacgtc gtcgcggggc cggatcgcgc
     2761 gctcgcgcag gccggcggcg gccagcagcg cggcgcaggc cagcgccagc acgttctggc
     2821 ccagcggccg gtcgaacagc cgggtcacga tgagcagggc ggcccagccc gccgcggcca
     2881 tgatcaccgt gccgtcgccg aagggcaggt ggaagtgccg tccctcggcg cgggcgcgca
     2941 gcagggcgag cacgcccagc gcggtcagga tcacgccggc ctcgaccagc gagaacgggc
     3001 cgaaggtgct gacgatcagc agcacggagg ccacggccgc caccttctgc tcgaagccga
     3061 gccggcgcca gagttgcaca acggtggcca cgccgggaag ggtacgcacg taggagcgca
     3121 gcgcgagcgt cggtcgcgcg agcaccgcac gaggcctgtt caggagcgct tggaggcgcg
     3181 gcggtgcttc ttcctggcca ccttgacctt gcccgcgcgg gtggccaggg gccgcccgga
     3241 ggcgtcgtag gcggccacgg tgaagcggta gcggccggcc ttcaggcgca tggtgcgccg
     3301 ctgggcggag gccatcttgg ccacgctgga ggcgtgcacg gcgaagctgc ggcggccgac
     3361 cttgacccgg tactgggcca ccgcgccgac ggcgccgcgg gcgcgccact tcagggcgat
     3421 gcgcccggtc ttggagcgga tcagcctgcg cgacgccagc gtcacgcgcg ggccggcgcc
     3481 cgggatcagc cgggtcatgg cccgcgaggc gtccagcgag ccgccgctgc ccaccatctg
     3541 cgcgtcgatg ctcatgtgcc gcacgccgcc caccagggcg tcgcggatct ggtcgggggt
     3601 cgccgtgggg cgggcggccg ccagcagggc ggcggcgccg ctgacctggg cggtcgcgat
     3661 cgaggtgccc gagctggagg cgtaggcgcc gcccctggcg gtggacagca cgtcctggcc
     3721 cggcgcggag agcagtaccg agcggccgta ggcggagaag gtggccaggc cgccgtcctg
     3781 ggactgcgcg gccaccggca gggtcaggcc cggggcggag gccgggtagg agggcgaggc
     3841 gtcgcggttg gcgccgtcgt tgccggcgga ggcgacgacc acgatgccgg cggccttggc
     3901 ggccgccagc gtctcctcga gcgcctggct gcggtcgggc ccgttcatgc tcaggttgat
     3961 cacgtcggcg ccgttcgcga tggcgtagcg caggccggtg atcaccgagc tggtgttgcc
     4021 ggagccgtcg tcggccagga ccttgaccgg catgatcttg gcgtcggggg cgacaccggt
     4081 gacgccgatg gtgttggcgg cggcggccgc gccgccgctc atcgcggtgc cgtggccgta
     4141 gc
//
LOCUS       DASUPW010000583         6798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1798311, whole genome shotgun sequence.
ACCESSION   DASUPW010000583 DASUPW010000000
VERSION     DASUPW010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6798
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1798311"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1565)
                     /locus_tag="VFY44_13190"
     CDS             complement(30..1565)
                     /locus_tag="VFY44_13190"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX5911442.1"
                     /translation="MQRMAVIDMGSNSFRLVVFEYEPTGWWSLIDEIREPTRVSAGMA
                     ESGALQPKPMKRAVQTAIVFSNFLRASGVERVEAVATSAIRDASNRDELLAEITERTG
                     LEVRVISGAEEAWYGYLAIANSTTVGDGFGIDIGGGSVQIMQLQGRRLLEAESVRLGA
                     VRVSEEFLPGEETTAKQIKALRKHVARTLSEFEWFNGGEQARLAGLGGTIRNLAAAAQ
                     KRLEVTDIDVQGFVLTREALGELIEELASRPADKRGSVRGIKPDRGDVILGGAVVLDA
                     AMEQGGFGGIEVTEAGLREGVLFERMLEGHEPPLMDDVRRASVENLEHRYHGDHAHDE
                     HVAKLSLEMFDALAAADLHDLGRFEREVLWAACLLHDIGVSIDYDDHHRHSHYLILNS
                     GLPGWSPRELELIALVARWHRKGAPDASRLGALERKGDGKRLKVLCGVIRMAEQLERS
                     RDRSVASVSLEQRKSAVRLVAATNGSGADPSVAVWAAQRGSDLLAEAIGQDVEVSLAD
                     DPG"
     gene            1660..3003
                     /locus_tag="VFY44_13195"
     CDS             1660..3003
                     /locus_tag="VFY44_13195"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX5911443.1"
                     /translation="MATTTSPTHALAGPALDNRRIAAALIDLAAPMLAAVALIATGLL
                     TPAVALVLAGWTLYYFFALESAGGQTIGKRAMNLKVVSADGGEPTMQQYAVRTLARVI
                     DTLGIGLLVMLGTGDRRQRLGDIAARTSVVDAQGGEGAGVDAFDAAHLHAQTAPPATT
                     SPEPEAKPKRSRPGLGGPELKMPSFGRSKAPKAPKEPKPAKVKSGRPSLGGPELKMPS
                     FGRSKAPKEPRPAKEKKGRPSLGGPELKMPSFGRKKNQVVVPEPEQVAPEPAPAPEPA
                     PAPEPTPAPEPVAPEPRVAQEAPAQPPVPEVKPFDPFAADAPEPSVEVVAHDEPDAEP
                     EAPEPPAPEIMERDAPEVEILRDDPPAEPPAPEPMRPSIPAVPPPPADEPLPAAAQHD
                     PARQHEPTPAPELEPEHVEGIRDDGSSRVNVKPIETVSAMELLMREAEEQDQHSR"
     gene            3028..4413
                     /locus_tag="VFY44_13200"
     CDS             3028..4413
                     /locus_tag="VFY44_13200"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5911444.1"
                     /translation="MAGTPDTPLRVAVVGSGPAGFYATGHLLAAKDVTVSVDLFDRLP
                     TPFGLVRAGVAPDHPKIKSVTRVYEKTAAKDGFRFFGNVEVGKHVTHDELKQRYHAVI
                     YAYGCERDRHMGIEGEDLPGSWAATEFVAWYNGHPDYQDLEFDLSAKRAVVIGNGNVA
                     MDVARMLVLPREELEGTDTTDAAIDAIAGSGVEEVVIVGRRGPAQAAFTNPELRELGE
                     LTDADVIVDPAEVELDDHSARSIEGDGQITHRRNVEILADYAGRTPQGKPKRVVLRFL
                     RSPVAIHGDGRVESIELVRNELEHGGDGRLRAQSTGEHETLEAGLVFRSIGYRGAPIE
                     GVPFDDWHGTIPNEEGRIIDPDTQGSIPGEYVAGWVKRGPSGVIGTNKRDAQETVEHV
                     LEDLGEGRLPEPADADPEALEALLRERQPDLVAYSGWEAIDAAEQAAGEPLGRPRVKF
                     TSVSEMLAAAR"
     gene            complement(4385..5083)
                     /locus_tag="VFY44_13205"
     CDS             complement(4385..5083)
                     /locus_tag="VFY44_13205"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEX5911445.1"
                     /translation="MANRTFVKFTFIRVQPEWRRREPGERAQDKREFAAACRDFAEDH
                     LLRTYSLVGTRGDTDLMVRTVAPSLDPIHDFHVLLNQSGLMRYAEVSHSYLAMTKESV
                     YSEEATPLEPRPGSDAKYLIVHPMWKKREWYLLDPKERWRIMQGHIETGRKYTGIEIN
                     TGYSYGLDDQEFVVSFNADEPGEFLDLVQELRGTESSAYTESETPIFTCISASPERAL
                     DALDGLSVPQPASR"
     gene            5128..>6798
                     /locus_tag="VFY44_13210"
     CDS             5128..>6798
                     /locus_tag="VFY44_13210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5911446.1"
                     /translation="MRVGIVSPYSYTYPGGVGRHVEALSGELSAQGHDVRIMAPYDPD
                     DRLARVMHRGAKPDSRPMPENLISLGRSTGFPANGSVSNLGISSQAVGTLGRELRHGG
                     YDVIHVHEPNAPFVSYFATEFSRVPLVGTFHCYSTSTLANGLASNVVGVRRLYNKIGV
                     RLAVSEAAQWTAERYYGGRYRIVPNGVDLAAARPDHGGSDALELLYVGRADARKGLPV
                     LLRAFEALRSAGVPARLTVAGATWEEVEPVLMDAEGVHVAGRVSDEEKWRLLGQADLL
                     VAPSLGGESFGMVLTEAFASNTPVVASDIVGYRDVVRHGYDGLLVPPADPAALGEALR
                     DLALDPERRAKMAVAARERAERFSWPTVTEEITEAYEHAVELPQPATRSQAVARRLGL
                     AAADGRPVVRPSRIPSIEPKLPRTGRRRAVRVARRAAVIGAAVGGVGLTALAVEHIGI
                     ESIGRAVVASSPVWILVAFALMCASMLMRAEAWHAILRAALPGVPLRRRDTARGTMIG
                     VFMSATLPARLGEPSRALIVARRAGRVRDRLPVVLGTLISQTILNLLAL"
BASE COUNT          923 a         2571 c         2391 g          913 t
ORIGIN      
        1 aacacgctgt tctgcctgct cccgcgccgc tagcccgggt cgtccgccag cgacacctcg
       61 acgtcctggc cgatggcctc ggccagcagg tccgacccgc gctgggcggc ccacaccgcg
      121 acgctggggt cggcgcccga gccgttggtg gcggccacga gccgcaccgc cgacttgcgt
      181 tgctccaggc tcaccgacgc caccgaccgg tcgcggctgc gctccagctg ctcggccatg
      241 cggatcacgc cgcacagcac cttcaggcgc ttgccgtcgc ccttgcgctc cagcgccccc
      301 agccgggagg cgtcgggcgc gcccttgcgg tgccagcgcg ccaccagcgc gatcagctcg
      361 agctccctcg gtgaccagcc cggcagcccg ctgttcagga tcaggtagtg ggagtggcgg
      421 tggtggtcgt cgtagtcgat cgacacgccg atgtcgtgca gcaggcaggc cgcccacagc
      481 acctcgcgct cgaagcggcc gaggtcgtgc aggtcggcgg cggccagggc gtcgaacatc
      541 tccagcgaca gcttggccac gtgctcgtcg tgcgcgtggt cgccgtggta acggtgctcc
      601 aggttctcga ccgaggcgcg cctgacgtcg tccatcagcg gcggctcgtg gccctcgagc
      661 atgcgctcga acagcacccc ctcgcggagg ccggcctcgg tcacctcgat gccgccgaag
      721 ccgccctgct ccatggccgc gtccagcacc accgcgccgc ccaggatcac gtcgccgcgg
      781 tcgggcttga tcccacgcac cgacccccgc ttgtccgccg ggcgcgaggc cagctcctcg
      841 atcagctcgc cgagcgcctc gcgggtcagc acgaagccct gcacgtcgat gtcggtgacc
      901 tcgaggcgct tctgggccgc ggcggccagg ttccggatcg tcccgcccag gccggccagg
      961 cgggcctgct cgccgccgtt gaaccattcg aactccgaca gcgtgcgcgc cacgtgcttg
     1021 cgcagcgcct tgatctgctt cgcggtcgtc tcctcgccgg gcaggaactc ctcgctgacg
     1081 cggaccgcgc ccagccgcac cgactccgcc tcgagcagcc gccggccctg cagctgcatg
     1141 atctgcacgc tgccgccgcc gatgtcgatc ccgaacccgt cgcccacggt cgtggagttg
     1201 gcgatggcca ggtagccgta ccaggcctcc tccgcgccgc tgatcacccg cacctcgagc
     1261 ccggtccgct cggtgatctc ggccagcagc tcgtcgcggt tggaggcatc gcggatggcg
     1321 ctcgtggcca ccgcctcgac ccgctccacc cccgacgcgc gcaggaagtt cgagaacacg
     1381 atggcggtct gcaccgcccg cttcatcggc ttgggctgga gggccccgga ctcggccatg
     1441 ccggcgctga cccgggtggg ctcgcggatc tcgtcgatca ggctccacca gcccgtgggc
     1501 tcgtactcga acaccaccag gcggaacgag ttggagccca tgtcgatgac ggccatgcgc
     1561 tgcatcggtg gccccatgtt gcccatagtc gcgccccagg tggggccagg cttgcagcag
     1621 ggctaaggat cgcgggagcc tctgccgata ccgagaccga tggctacgac cacctcgcct
     1681 acccacgcgc tcgcaggtcc cgccctggac aaccgccgga tcgcggcggc actgatcgac
     1741 ctggccgcgc cgatgctggc cgccgtcgcg ctgatcgcga ccgggctgct gacgcccgcg
     1801 gtggcgctgg tgctggccgg gtggaccctc tactacttct tcgcgctcga gagcgccggc
     1861 ggccagacca tcggcaagcg cgccatgaac ctcaaggtcg tgtccgccga cggcggcgag
     1921 ccgaccatgc agcagtacgc cgtgcgcacg ctcgcgcgcg tgatcgacac cctgggcatc
     1981 ggcctgctgg tcatgctcgg cacgggcgac aggcgccagc gcctgggcga catcgcggcc
     2041 cggacgtcgg tggtcgacgc ccagggcggc gagggggcgg gtgtcgacgc gttcgacgcc
     2101 gcccacctgc acgcccagac cgcgcccccg gcgaccacgt cccccgagcc cgaggccaag
     2161 cccaagcgct cccgccccgg cctcggcgga ccggagctga agatgccgtc gttcggccgc
     2221 tcgaaggcgc cgaaggcccc gaaggagccc aagccggcga aggtgaagtc cggccggccg
     2281 tcgctgggtg gcccggagct gaagatgccg tccttcggcc gctcgaaggc gccgaaggag
     2341 cccaggcccg ccaaggagaa gaagggccgg ccctcgctgg gtggccccga gctgaagatg
     2401 ccgtcgttcg gccgcaagaa gaaccaggtc gtggtgcccg agcccgagca ggtcgcgccc
     2461 gagccggccc ccgcgcccga gccggcgccg gcccccgagc cgactcccgc ccccgagccg
     2521 gtcgcccccg agccccgggt cgcgcaggag gcgcccgcgc agcccccggt gcccgaggtc
     2581 aagcccttcg acccgttcgc cgccgacgcg cccgagccct cggtcgaggt ggtggcccac
     2641 gacgagcccg acgccgagcc cgaggccccg gagccgcccg cgcccgagat catggagcgc
     2701 gacgccccgg aggtggagat cctccgcgac gacccgccgg ccgagccccc cgcgcccgag
     2761 ccgatgcggc cgtcgattcc cgcggtcccg cccccgcccg ccgacgagcc ccttccggcc
     2821 gcggcccagc acgaccccgc ccggcagcac gagccgacgc ccgcgcccga gctcgagccg
     2881 gagcacgtgg aggggatccg cgacgacggc tcctcgcgcg tgaacgtgaa gccgatcgag
     2941 acggtctcgg ccatggagct gctgatgcgc gaggccgaag agcaggacca gcacagccgc
     3001 tgaccgccgc taacatcgcc cgccgctgtg gcgggcaccc ctgacactcc cctgcgcgtt
     3061 gcggtcgtcg gctccggccc cgcgggcttc tacgcgaccg ggcacctgct ggccgcgaag
     3121 gacgtgaccg tgtcggtcga cctgttcgac cggctgccga cccccttcgg gctggtgcgg
     3181 gcgggcgtcg cgcccgacca ccccaagatc aagtcggtca cgcgcgtcta cgagaagacc
     3241 gccgccaagg acggcttccg cttcttcggc aacgtcgagg tgggcaagca cgtcacccac
     3301 gacgagctca agcagcgcta ccacgcggtg atctacgcct acggctgcga gcgcgaccgg
     3361 cacatgggga tcgagggcga ggacctgccc gggagctggg ccgccaccga gttcgtggcc
     3421 tggtacaacg gccacccgga ctaccaggac ctggagttcg acctctcggc caagcgcgcc
     3481 gtggtcatcg ggaacggcaa cgtggccatg gacgtggccc ggatgctggt cctgccgcgc
     3541 gaggagctcg agggcacgga caccaccgac gcggccatcg acgcgatcgc cgggagcggg
     3601 gtggaggagg tcgtgatcgt cggccggcgc ggtcccgccc aggccgcgtt caccaacccc
     3661 gagctgcgcg agctgggcga gctgaccgac gccgacgtga tcgtcgaccc ggccgaggtc
     3721 gagctggacg accacagcgc acggtcgatc gagggcgacg gccagatcac ccaccggcgc
     3781 aacgtcgaga tcctggccga ctacgcgggc cgcacgcccc agggcaagcc caagcgcgtg
     3841 gtgctccgct tcctgcgctc gccggtcgcc atccacggcg acggcagggt ggagtcgatc
     3901 gagctggtcc gcaacgagct ggagcacggc ggggacgggc gcctgcgggc gcagtccacc
     3961 ggcgagcacg agacgctgga ggcgggcctg gtgttccgct cgatcggcta ccgcggggct
     4021 cccatcgagg gcgtcccgtt cgacgactgg cacgggacca tccccaacga ggaggggcgg
     4081 atcatcgacc ccgacaccca gggctcgatc cccggcgagt acgtggccgg gtgggtcaag
     4141 cgcggcccct cgggcgtgat cggcaccaac aagcgcgacg cccaggagac ggtcgagcac
     4201 gtgctcgagg acctgggcga ggggcggctg cccgagcccg ccgacgcgga ccccgaggcc
     4261 ctggaggcgc tcctgcgtga gcgccagccg gacctggtgg cctactccgg ctgggaggcc
     4321 atcgacgccg ccgagcaggc ggccggcgag ccgctgggcc ggccgcgcgt gaagttcacc
     4381 tcggtcagcg agatgctggc tgcggcacgc tgaggccgtc gagcgcgtcc agggcgcgct
     4441 ccggggaggc cgagatgcag gtgaagatgg gcgtctcgga ctcggtgtag gcgctggact
     4501 ccgtgccccg cagctcctgc accaggtcca ggaactcgcc cggctcgtcg gcgttgaacg
     4561 acaccacgaa ctcctggtcg tccaggccgt aggagtagcc ggtgttgatc tcgatgccgg
     4621 tgtacttgcg gccggtctcg atgtggccct gcatgatccg ccagcgctcc ttggggtcca
     4681 gcaggtacca ctcgcgcttc ttccacatcg ggtggacgat caggtacttg gcgtccgagc
     4741 cggggcgcgg ctccagcggc gtggcctcct cggagtacac ggactccttg gtcatggcca
     4801 ggtacgagtg gctgacctcg gcgtagcgca tcaggccgct ctggttcagc agcacgtgga
     4861 aatcgtggat cgggtccagc gacggcgcca ccgtgcgcac catcaggtcg gtgtccccgc
     4921 gggtgcccac cagcgagtag gtgcgcagca ggtggtcctc ggcgaagtcc ctgcaggcgg
     4981 cggcgaactc gcgcttgtcc tgggcccgtt cgcccggctc gcgccggcgc cactccggct
     5041 ggacgcggat gaacgtgaac ttcacgaagg tgcggttagc cacacaagga gtgtggcgca
     5101 tccggagggg tggtctacga tggctcggtg cgcgtaggga tcgtttctcc gtactcgtac
     5161 acgtacccag gtggtgtagg gcgccatgtc gaggccctct ccggggagct gtcagcccag
     5221 ggacacgatg tccggatcat ggccccgtac gacccggacg accgcctggc tcgtgtaatg
     5281 catcgcggcg cgaagccgga tagccgcccc atgcccgaga acctgatctc gctcgggcgc
     5341 tccacgggct tccccgccaa cggctcggtc tcgaacctgg ggatctcctc ccaggcggtc
     5401 ggcacgctgg ggcgcgagct gcgtcacggc ggctacgacg tgatccacgt gcacgagccc
     5461 aacgcgccgt tcgtgagcta tttcgccacc gagttctccc gcgtgcccct ggtgggcacc
     5521 ttccactgct actcgaccag cacgctggcc aacggcttgg cctcgaacgt ggtcggcgtg
     5581 cgccggctct acaacaagat cggcgtgcgc ctggccgtgt ccgaggccgc ccagtggacc
     5641 gccgagcgct actacggcgg ccgctaccgg atcgtcccca acggcgtcga cctggccgcg
     5701 gccaggcccg accacggcgg ctccgacgcg ctcgagctgc tgtacgtggg ccgcgccgac
     5761 gcgcgcaagg gcctgcccgt gctgctgcgc gccttcgagg ccctgcgcag cgccggcgtg
     5821 cccgcccgcc tgaccgtggc cggcgccacg tgggaggagg tcgagccggt gttgatggac
     5881 gccgagggcg tgcacgtggc cggccgcgtc agcgacgagg agaagtggcg cctgctgggc
     5941 caggccgacc tgctggtcgc gccgtcgctg ggcggcgaga gcttcggcat ggtgctgacc
     6001 gaggcgttcg cctccaacac cccggtcgtg gcctccgaca tcgtcggcta ccgcgacgtg
     6061 gtccggcacg gctacgacgg cctgctggtc ccgcccgccg accccgccgc gctgggcgag
     6121 gccctgcgcg acctggcgct ggaccccgag cgccgggcca agatggccgt ggccgcgcgc
     6181 gagcgggccg agcgcttctc ctggcccacc gtgaccgagg agatcaccga ggcctacgag
     6241 cacgcggtcg agctgcccca gccggccacc cggtcgcagg ccgtggcgcg ccggctcggc
     6301 ctggccgccg ccgacggccg gcccgtggtg cggcccagcc ggatcccctc gatcgagccc
     6361 aagctcccgc gcacgggccg gcgccgcgcc gtgcgggtcg cccgccgcgc cgccgtcatc
     6421 ggcgccgccg tcggcggcgt cgggctgacc gccctggccg tggagcacat cggcatcgag
     6481 tccatcggcc gcgcggtcgt ggcctcgtcg cccgtgtgga tcctggtcgc gttcgcgctc
     6541 atgtgcgcgt ccatgctcat gcgcgccgag gcctggcacg cgatcctgcg cgccgccctg
     6601 ccgggcgttc cgctgcgccg ccgcgacacc gcccgcggca ccatgatcgg cgtcttcatg
     6661 tcggccacgc tgccggcccg cctcggcgag ccctcacgcg cgctgatcgt ggcccgccgc
     6721 gcgggccgcg tgcgcgaccg attgccggtg gtccttggca cgctgatctc gcagacgatc
     6781 ctgaacctgc tggcgctg
//
LOCUS       DASUPW010000584         2355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_413546, whole genome shotgun sequence.
ACCESSION   DASUPW010000584 DASUPW010000000
VERSION     DASUPW010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413546"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..552
                     /locus_tag="VFY44_13215"
     CDS             43..552
                     /locus_tag="VFY44_13215"
                     /inference="COORDINATES: protein motif:HMM:NF025196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4188 domain-containing protein"
                     /protein_id="HEX5911447.1"
                     /translation="MGKIHRGRYSAEIEGDFVVLLIGARLNKPWLALQSLADLGGRRR
                     GMVSMLKELSAHPEKGLLGYRMGFSVIVQYWRSFEHLEAFARDPGDLHRPTWLDWYRR
                     DPQGRTGIWHETYLVRAGEYEAIYSDVPEAGLAAAGRAVPLTGNSSARLRLRRAAAEG
                     TPNTTPAVS"
     gene            624..1811
                     /locus_tag="VFY44_13220"
     CDS             624..1811
                     /locus_tag="VFY44_13220"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEX5911448.1"
                     /translation="MLPSDAQHGVTTLELFFDLVFVFAITQVTAFMADDLGWRGALRG
                     LVLLAILWWAWCSYAWLGNQVHADEGLVRAALVVAMSALFLVALALPEAWGDEGGGIS
                     APVVLAVAFAVVRGVHLGVYAVAAVGDAGLRRQLLRTALPVGVAAVLLVVGALLGGVA
                     QTALWALALVVDYTGVYLSGTDWRLPSPRHFAERHGLIVIIALGESIIAVGVGVADLP
                     ITLPIIAAALLGLAVSVALWWLYFDVVAPVAERVLSSKEGTERVRLARDSYTYLHFPM
                     IAGIIYLALGLKKVAEYVADTEHHALTDPLKGTGLWALYGGVAVYLIAHLAFRMRNVG
                     SINRPRAVVAVLLLVAPLGVRYLPALAGLGVLALILGALIAFEVVRYAEARAAIRAEA
                     AHH"
     gene            complement(1816..>2355)
                     /locus_tag="VFY44_13225"
     CDS             complement(1816..>2355)
                     /locus_tag="VFY44_13225"
                     /inference="COORDINATES: protein motif:HMM:NF012611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911449.1"
                     /translation="KVLAAPGEAPADITDREDFVEAVRQALYASKVVAYAQGFDQIAA
                     GALANNWNIDLGALATIWRGGCIIRARFLDRIRQAFADEPTLTTLLTHGYFRDAVAAG
                     QASWRRVVIEAAQSGIPAPGFASSLAYYDGLRAERSSAALIQGLRDFFGSHTYQRVDR
                     AGAFHTLWAEEGRREVTAG"
BASE COUNT          280 a          837 c          870 g          368 t
ORIGIN      
        1 cgtcacccac gggggccgtt gccacatccg gagggagtcc gcgtgggaaa gatccaccgt
       61 ggccgctaca gcgccgagat cgagggcgac ttcgtggtcc tcctcatcgg tgcgcggctg
      121 aacaagccct ggctcgcgct gcagagcctt gccgacctcg gggggcgccg gcggggaatg
      181 gtcagcatgc tgaaggagtt gtccgcccat cccgagaagg gtctgctcgg ttaccggatg
      241 ggcttctccg tgatcgtgca gtactggcgc agcttcgagc atctcgaggc attcgcccgc
      301 gaccccggtg atctgcaccg gccgacctgg ctggactggt atcggcgcga cccccagggc
      361 cggaccggca tctggcacga gacgtacctg gtacgggccg gcgaatacga ggcgatctac
      421 tccgacgtgc cggaggcggg cctggcggcg gccggccggg ccgtgccgct gaccggcaac
      481 agttctgcca ggctgcggct gcggcgggcg gcggcggagg ggacgccgaa cacgaccccc
      541 gccgtctcgt gacggcggag ccgtccgagt ccgccgcgcc cgtggattct cgggggtcac
      601 gcccgacccg gccccgtgcg cgcgtgcttc ccagcgacgc ccagcacggg gtcaccaccc
      661 tcgagctctt cttcgacctc gtcttcgtct tcgccatcac ccaggtgacc gcgttcatgg
      721 ccgacgacct cgggtggcgc ggagcgctgc gcggcctcgt gctgctggcg atcctctggt
      781 gggcctggtg cagctacgcc tggctgggca accaggtgca cgccgacgag ggcctcgtcc
      841 gcgccgctct cgtggtcgcg atgtcggcac tgttcctggt cgccctggcc ctaccggagg
      901 cgtggggcga cgagggcggc gggatcagcg cgccggtcgt gctggcggtc gcgttcgcgg
      961 tggtccgcgg ggtgcacctc ggggtctacg ccgtcgcggc ggtcggcgac gcgggtctgc
     1021 gccgtcagct gctccgcacc gccttgccgg tcggcgtcgc ggccgtcctc ctggtggtgg
     1081 gcgcgctcct gggcggggtc gcgcagaccg cgctctgggc gctggccctc gtcgtcgact
     1141 acaccggcgt ctacctgtcg ggcaccgact ggcggctgcc ctcgccccgg cacttcgccg
     1201 agcggcacgg cctgatcgtg atcatcgccc tgggggagtc gatcatcgcc gtcggcgtcg
     1261 gggtggccga cctgccgatc acgctgccga tcatcgcggc cgccctgctg ggcctggcgg
     1321 tcagcgtggc gctgtggtgg ctgtacttcg acgtggtggc gccggtggcc gagcgggtgc
     1381 tgagcagcaa ggagggcacc gagcgggtgc ggctggcccg cgacagctac acgtacctgc
     1441 acttcccgat gatcgccggg atcatctacc tcgccctcgg gctgaagaag gtcgccgagt
     1501 acgtggccga caccgagcac cacgcgctca ccgacccgct caagggcacc ggcctctggg
     1561 cgctgtacgg cggcgtggcg gtgtacctga tcgcccacct ggcgttccgc atgcgcaacg
     1621 tcggctccat caaccggccg cgggccgtcg tggccgtcct gctgctcgtc gcgccgctcg
     1681 gtgtgcggta tctgcccgcc ctggcggggc tgggcgtgct ggcgttgatc ctcggcgcgc
     1741 tcatcgcctt cgaggtggtg cgctacgccg aggcgcgggc ggccatccgc gcggaggcgg
     1801 cccaccactg accggtcagc cggccgtgac ctcccggcgg ccttcctcgg cccagagcgt
     1861 gtggaaggcg ccggctcggt cgacccgttg gtaggtgtgc gagccgaaga agtcccgcag
     1921 gccctggatg agtgccgcgg acgaccgctc ggcccgcaga ccgtcgtagt acgccagcga
     1981 gctggcgaag ccgggagccg ggatgccgct ctgcgccgcc tcgatcacca cgcgccgcca
     2041 gctcgcctgg ccggcggcca ccgcgtcgcg gaagtagccg tgcgtcagca gcgtggtcag
     2101 ggtgggctcg tcggcgaacg cctgccggat ccggtcgagg aaccgggcgc ggatgatgca
     2161 gccaccgcgc cagatggtgg ccagggcgcc gaggtcgatg ttccagttgt tcgccagcgc
     2221 acccgcggcg atctggtcga agccctgggc ataggcgacc accttcgagg cgtagagcgc
     2281 ctgccggacg gcctccacga aatcctcgcg gtccgtgatg tccgcgggcg cctcgccggg
     2341 cgcggccagt acctt
//
LOCUS       DASUPW010000585         3009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1936890, whole genome shotgun sequence.
ACCESSION   DASUPW010000585 DASUPW010000000
VERSION     DASUPW010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1936890"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VFY44_13230"
     CDS             complement(<1..654)
                     /locus_tag="VFY44_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911450.1"
                     /translation="MPTTSAPEPRSLAVPLKALLARRVADGGDSGWWRALIPALAIIA
                     VLFAALEVRSASEAADRALARSADAQEVAATVDRLAILSRAALDEGRSSRTLEAARER
                     VLAESDAATRGGGDGSPAADVAQQAALADGALDTALARGATAAQRRRADVALADLQPA
                     ARAYSDRAEAAATRADDRAVARLTWTLIGVLVLVTLLLGSFWSRRNAVDALRRERRFH
                     "
     gene            complement(729..1862)
                     /locus_tag="VFY44_13235"
     CDS             complement(729..1862)
                     /locus_tag="VFY44_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911451.1"
                     /translation="MRIRLPALCAGLVLALLAPATASAAGFPAIQGPFPFQLQKGGFN
                     ENPVIGGSGYQRQSGPMSVFKSGNRFPAGLDAGTRKRVGVPANGAGFRWNEGDDRVDY
                     WIPQGLTGSADAGPERAAAYPGALLASWYDDSGNRARISFVSGLRDDRRLSYRHVLLV
                     QPDGRGSYTNVDTHAGGLAWFRNRLYVADTDGIRVFDMRRIYRVKPGGGRLKKSGDYA
                     YVMPQIGSYGSPGLRISFVAVDSAGSPALVAGEYKEGAGAGGRIARFPLDGGTGDLVT
                     NLGADRVGALGAFGSPRGSMQGAVTRGNRVWVSGSTGKDKRGALVYGTVGRRRSKSLP
                     WLVGAEDLMWSGGRLYSLSEYRWEKRRFLPDRRGRVVVSVPAP"
     gene            1930..>3009
                     /gene="uvrC"
                     /locus_tag="VFY44_13240"
     CDS             1930..>3009
                     /gene="uvrC"
                     /locus_tag="VFY44_13240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX5911452.1"
                     /translation="MSATDTPQTRSERIRAKRLALPDAKGVYLFKDERGKVIYVGKAT
                     SIRKRVAGHFSKPSTRGALEMTQTVADFDFLVTETEAEALLTEQNFIKRHRPRYNVRL
                     RDDKSYPYIGISMEEDYPRVFFTREKHRRGRAYFGPFSSAKRVRETLELLGKVFQYRT
                     CQGKEPGRQSGSPCLDYFIKRCQAPCVDYISKEEYRQNIETIIDFLSGRYRRIERELE
                     QGMQAASADQEFEAAAQLRNKLRAVRSLLERQRVTNDSVGTLDAVAVAAEETSANAQV
                     FQVRDGVLADRQSFYLENEAGREEPEVLEEFLLQYYASAMAIPPRVVVPRGLEDPAAL
                     EAALSTQRGGPVEVRPAERGDKRRIF"
BASE COUNT          422 a         1095 c         1123 g          369 t
ORIGIN      
        1 gtggaaccgg cgctcgcgac ggagggcgtc gacggcgttg cgtcgtgacc agaagctccc
       61 cagcagcagc gtgaccagca ccagcactcc gatcagcgtc caggtcagcc gtgcgacggc
      121 ccggtcgtcg gcgcgggtcg cggccgcctc ggcgcggtcg ctgtacgccc gcgccgcggg
      181 ctgcaggtcg gccagggcga cgtcggcgcg ccgccgctgg gcggcggtgg ccccacgtgc
      241 gagggcggtg tccagggcgc cgtcggccag ggcggcctgc tgcgccacgt ccgcggccgg
      301 gctgccgtcg ccgccgcctc gggtggccgc gtcggactcg gcgagcaccc gctcccgggc
      361 cgcctcgagc gttcgcgagc tgcggccctc gtccagggcg gcgcggctca ggatcgccag
      421 ccggtcgacg gttgcggcca cctcctgcgc gtcggcggag cgggccagcg cgcgatcggc
      481 tgcctcgctg gccgagcgca cctccagggc cgcgaacagg acggcgatga tcgccagggc
      541 cgggatcagc gcacgccacc agccgctgtc gcccccgtcg gccacgcggc gcgccagcag
      601 ggccttcagc gggaccgcga gcgatctggg ctcaggggcg gaggtcgtgg gcatcgcgga
      661 cccatcggcc gccgcggggc cgcgcttgac gacgcgcggc cgggctggac ggtcgaccga
      721 gggcccggtc agggcgccgg gaccgacacc acgacccgcc cgcggcggtc gggcaggaac
      781 cgccgcttct cccagcggta ctcgctgagg ctgtacaggc ggccgccgga ccacatcagg
      841 tcctcggctc cgacgagcca cggaagcgac ttcgagcgcc tgcggccgac ggtcccgtag
      901 accagcgcgc cgcgcttgtc cttgccggtg ctgcccgaca cccagacgcg gttcccgcgc
      961 gtgaccgcgc cctgcatgct cccgcgcggc gagccgaacg cgccgagcgc gccgacccgg
     1021 tcggcgccca ggttggtcac caggtcgccg gtgcccccgt ccagcgggaa gcgggcgatg
     1081 cgcccgccgg cgccggcgcc ctccttgtac tcgccggcca ccagcgcggg cgagcccgcc
     1141 gagtccacgg ccacgaacga gatccgcagg ccggggctgc cgtaggagcc gatctgcggc
     1201 atcacgtagg cgtagtcgcc gctcttcttc agccgcccgc cgccgggctt gacgcggtag
     1261 atacgccgca tgtcgaacac gcggatgccg tccgtgtcgg ccacgtacag gcggttgcgg
     1321 aaccaggcca ggccgcccgc gtgggtgtcc acgttggtgt agctgccgcg gccgtcgggc
     1381 tggaccagca gcacgtgccg gtaggacagt cggcggtcgt cgcgcaggcc ggagacgaac
     1441 gagatccggg cccggttgcc ggaatcgtcg taccaggagg ccagcagcgc gcccgggtag
     1501 gcggcggcgc gctcggggcc cgcgtcggcg gacccggtca ggccctgggg gatccagtag
     1561 tccacgcggt cgtcgccctc gttccagcgg aacccggcgc cgttcgcggg cacgcccacg
     1621 cgcttgcggg tcccggcgtc caggccggcc gggaaccggt tgcccgactt gaacacggac
     1681 atcggcccgc tctggcgctg gtagccggag ccgccgatca cggggttctc gttgaagccg
     1741 cccttctgca gctggaaggg gaacgggccc tggatcgcgg ggaatccggc ggccgacgcg
     1801 gtcgcggggg cgaggagggc caggacgagg ccggcgcaca gggccggaag gcggatacgc
     1861 atgtgccggt ttctctccag cccccggtgg tttccaaacc cgcggcgcgc gtaatctcga
     1921 ctgagtcccg tgagcgccac cgacacaccc cagacccgca gcgagcgcat ccgcgcgaag
     1981 cgcctggcgc tgcccgacgc gaagggcgtc tacctgttca aggacgagcg cggcaaggtc
     2041 atctacgtcg gcaaggccac ctcgatccgc aagcgcgtgg ccggccactt ctcgaagccg
     2101 tccacccggg gcgcgctgga gatgacccag acggtcgcgg acttcgactt cctggtcacc
     2161 gagaccgagg ccgaggcgct gctgaccgag cagaacttca tcaagcgcca ccgcccccgc
     2221 tacaacgtgc ggctgcgcga cgacaagtcg tacccgtaca tcgggatctc gatggaggag
     2281 gactacccgc gggtgttctt cacgcgcgag aagcaccgcc gcgggcgggc ctacttcggc
     2341 ccgttctcca gcgccaagcg ggtgcgcgag accctggagc tgctgggcaa ggtgttccag
     2401 taccgcacct gccagggcaa ggagcccggg cgccagtccg gcagcccgtg cctggactac
     2461 ttcatcaagc gctgccaggc accgtgcgtg gactacatct ccaaggagga gtaccggcag
     2521 aacatcgaga ccatcatcga cttcctgtcg gggcgctacc ggcggatcga gcgggagctg
     2581 gagcagggca tgcaggcggc ctcggccgac caggagttcg aggcggcggc ccagctgcgc
     2641 aacaagctgc gggcggtgcg ctcgctgctc gagcgccagc gcgtgaccaa cgactccgtg
     2701 ggcacgctcg acgcggtggc ggtggcggcc gaggagacct cggccaacgc ccaggtgttc
     2761 caggtccgcg acggcgtgct ggccgaccgg cagagcttct acctggagaa cgaggcgggc
     2821 cgcgaggagc cggaggtgct ggaggagttc ctgctccagt actacgccag cgcgatggcc
     2881 atcccgccgc gcgtggtggt gcccagggga ctggaggacc cggccgcgct ggaggccgcg
     2941 ctgtccacgc agcggggcgg cccggtcgag gtccggcccg ccgagcgcgg cgacaagcgg
     3001 cgcatcttc
//
LOCUS       DASUPW010000586         2716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_1382061, whole genome shotgun sequence.
ACCESSION   DASUPW010000586 DASUPW010000000
VERSION     DASUPW010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382061"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..1264)
                     /locus_tag="VFY44_13245"
     CDS             complement(182..1264)
                     /locus_tag="VFY44_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911453.1"
                     /translation="MDSKHPRAGTMTRRTAALAAVAAVMLAVGVAAVLGGSDAPERAP
                     GPSPSGTPATTSPPAVPAQPAPAPDAPVPDTRLAALGGAGPLIGMADNRPETMTDPRF
                     GATGIKRVRVTVPFDDIALGGARLAIQDAWFSAARGQGIEPVVTFFRSSRGKTILPSP
                     EEFRRHFRLFRERYPWVRWFSTWNEANFSAQPTSRDPARTARFYRIARQECSGGRCEV
                     ITCDFRPDGRARSDRWLAAFKRGIGPGPHRWGLSSYVDVNRQSTTLTQRFLARTEGPV
                     WVNEVGAINFFGQGFAPDIQRQSRVMAYLLSTYAQVSPRIERIYVYHWRAAAGDDLFD
                     SGLLDVQGVPRPAYYLFFQAIGRPPA"
     gene            1308..2627
                     /gene="miaB"
                     /locus_tag="VFY44_13250"
     CDS             1308..2627
                     /gene="miaB"
                     /locus_tag="VFY44_13250"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX5911454.1"
                     /translation="MPSYHLTTFGCQMNEHDSERMKGMLESLGYAEAPARDGADLILF
                     NTCSIREKADTRLVGHLGEAKRLKAEHPERVIGVGGCWSQSMKEQVFELFPFVDVAFG
                     PGQVHKLAEFLTSDSLTAQGYFEFEGFTGHLPMRRARDFQGWMQISVGCNCRCAYCIV
                     PSTRGREVSRPAHELVAEAERLAGDGVREITLLGQNVNSYGRDLPKGAKTTFAELLAM
                     VDGVEGIDRIRYTSPHPKDMREDVVAAHVELESLCEHIHLPLQAGSSRVLKAMRRTYN
                     RERYMDRVAMIRAAVPDVALTTDIIVGFPGETEAEFAETLEVAEEVGYDSAFTFIYSP
                     RRETTAADLPDQVSHEVKRERMERLVEVVQRRATERSQRFVGRTMEVLVEGNSRTDED
                     RLFGRTRHNKSVHFDGLAQPGDMVQVEIESATSQTLAGSERLLSRVS"
BASE COUNT          401 a          922 c         1038 g          355 t
ORIGIN      
        1 tgggggcctg ggcctgtgtg cgggccggct cccgggtccc accgtcgtcg tcatcgccac
       61 gggtcaggga gaacgcgacc ccgccggcca gcagcgccgt ggccagcgcg gccaggatga
      121 tcgtgcggcg gggcattcag cgcgagcgta gacaacgcgc gtacgcatga cctggccggg
      181 atcaggcggg cgggcggccg atggcctgga agaacaggta gtaggcgggc cgcggtacgc
      241 cctgcacgtc cagcaggccc gagtcgaaca ggtcgtcgcc ggcggccgct cgccagtggt
      301 agacgtagat ccgctcgatc cggggcgaca cctgggcgta ggtggacagc aggtaggcca
      361 tcacccggct ctggcgctgg atgtcggggg cgaagccctg gccgaagaag ttgatcgccc
      421 cgacctcgtt cacccacacc gggccctcgg tccgggccag gaagcgctgc gtcagggtgg
      481 tggattggcg gttcacgtcc acgtacgacg acaggcccca gcggtggggc ccggggccga
      541 tgccgcgctt gaacgccgcc agccagcgat cggagcgcgc gcgcccatcg gggcggaagt
      601 cgcaggtgat gacctcgcag cgcccgcccg agcactcctg gcgggcgatc cggtagaagc
      661 gcgcggtccg cgccggatcg cgcgaggtgg gctgggcgct gaagttggcc tcgttccagg
      721 tcgagaacca ccggacccac gggtagcgct cgcggaacag gcggaaatgc cgccggaact
      781 cctccggcga gggcaggatg gtcttgcccc gcgacgagcg gaagaaggtg accaccggct
      841 cgatgccctg cccgcgggcg gcggagaacc aggcgtcctg gatcgccagc cgggcgccgc
      901 ccagggcgat gtcgtcgaag ggcacggtca cgcgcacgcg tttgatcccg gtggcgccga
      961 accgggggtc ggtcatggtc tcgggccggt tgtcggccat cccgatcagc gggccggcgc
     1021 cacccagggc cgcgagccgc gtgtccggca cgggggcgtc cggggcgggc gccggctggg
     1081 ccgggaccgc gggcggactg gtggtggccg gcgtcccgga cggggacggg ccgggtgcgc
     1141 gctcgggagc gtccgagccg cccagcacag cggccacgcc gacggcgagc atcacggccg
     1201 cgacggcggc gagggctgcg gtgcggcggg tcatggttcc ggcgcggggg tgcttcgagt
     1261 ccaacgcagc agggtaggga agccaggccc ggcggtatcc tgccgcgatg cccagctacc
     1321 acctgaccac cttcgggtgc cagatgaacg agcacgactc cgagcgcatg aagggcatgc
     1381 tggagtccct ggggtacgcc gaggctcccg cacgcgacgg cgccgacctg atcctgttca
     1441 acacctgctc gatccgcgag aaggcggaca cgcgcctggt ggggcacctg ggtgaggcca
     1501 agcggctcaa ggccgagcat cccgagcgcg tgatcggcgt ggggggctgc tggtcgcagt
     1561 cgatgaagga gcaggtgttc gagctgttcc cgttcgtgga cgtggccttc gggcccggcc
     1621 aggtgcacaa gctggccgag ttcctgacct ccgacagcct gaccgcccag gggtacttcg
     1681 agttcgaggg cttcaccggc cacctgccga tgcgccgcgc gcgcgacttc cagggctgga
     1741 tgcagatctc ggtgggctgc aactgcaggt gcgcctactg catcgtgccc tcgacccggg
     1801 ggcgcgaggt ctcgcggccc gcgcacgagc tcgtggcgga ggcggagcgc ctggccggcg
     1861 acggggtgcg cgagatcacc ctgctgggcc agaacgtgaa ctcctacggc cgcgacctgc
     1921 ccaagggggc caagacgacc ttcgccgagc tgttggccat ggtcgacggc gtcgagggca
     1981 tcgaccggat ccggtacacc agcccgcacc ccaaggacat gcgcgaggac gtcgtggcgg
     2041 cccatgtgga gctcgagtcg ctgtgcgagc acatccacct gccgctgcag gccggctcgt
     2101 cgagggtgct caaggccatg cgccgcacgt acaaccgtga gcgctacatg gaccgcgtcg
     2161 ccatgatccg cgccgcggtg cccgacgtgg cgctgaccac cgacatcatc gtgggcttcc
     2221 cgggcgagac cgaggcggag ttcgccgaga cgctggaggt ggccgaggag gtgggctacg
     2281 actcggcctt caccttcatc tactcgcccc ggcgcgagac cacggcggcc gacctgcccg
     2341 accaggtgtc gcacgaggtc aagcgcgagc ggatggagcg gctggtcgag gtggtccagc
     2401 gccgggcgac cgagcgctcc cagcggttcg tgggccgcac catggaggtg ctggtggagg
     2461 gcaactcgcg caccgacgag gaccgcctgt tcgggcgcac ccgccacaac aagagcgtcc
     2521 acttcgacgg cctggcgcag ccgggcgaca tggtccaggt cgagatcgag tcggccacca
     2581 gccagaccct ggccgggtcc gagcgcctgc tctcccgcgt ctcctagtcc gatggcggtg
     2641 ccggtgatcg ccgtgtgcgg ccccacgggg gtgggcaaga ccgccctggc gctggccctg
     2701 gccgaccggc tgcgcg
//
LOCUS       DASUPW010000587         8398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_275700, whole genome shotgun sequence.
ACCESSION   DASUPW010000587 DASUPW010000000
VERSION     DASUPW010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8398
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275700"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /gene="aroF"
                     /locus_tag="VFY44_13255"
     CDS             complement(<1..798)
                     /gene="aroF"
                     /locus_tag="VFY44_13255"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX5911455.1"
                     /translation="MREDATPEDVSEVVSRVESTGAKAHVIEGAAQQVIGVIGDVELV
                     RDLALEVAPGVDHTMPISRPFKLASSQVTHGKRTVIDIAGRKIGGDHFATIAGPCTVE
                     SRDVMLDSAHVVKAAGAQLLRGGAYKPRTSPYSFQGLGQEGLKLLAEAKQATGMPIVT
                     EVMDVRDLDAVCEVADMIQLGARNMQNYTLLTEIGRAGVPVLIKRGLSATLEELLMAA
                     EYVLKEGNGDVVLCERGIRTFETAYRFTLDLMAVPVLKDLTHLPVIVD"
     gene            1004..1855
                     /locus_tag="VFY44_13260"
     CDS             1004..1855
                     /locus_tag="VFY44_13260"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5911456.1"
                     /translation="MIAFLGTGIMGAPMARNTAAAGHDVRVWNRTRAKAEAVDGVQVA
                     GSPEEAVAGADVVVTVLADGDAVTAAMEDRGALAAMADDALWLQMSTVGIAATEALAA
                     LAEERSVPFVDAPVLGTKAPAEKGALTVLASGPGDVHERCAPVFDAVGAKTVWLGEAG
                     TATRAKLVLNHWVLSLTQAVAETVGLAEALDIDPQLFLDSIEGGPLDSGYAQMKGGAI
                     IARSFDPSFTLSLARKDAGLVLEAAERHEHEAALAEVVERQMTKAIEAGHGDEDMAAT
                     YFASRPQ"
     gene            complement(2010..3089)
                     /gene="hisC"
                     /locus_tag="VFY44_13265"
     CDS             complement(2010..3089)
                     /gene="hisC"
                     /locus_tag="VFY44_13265"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX5911457.1"
                     /translation="MSPGIEFNRRLGDIPSYPQAGSYDFGGELVKLASNESPFGPPAA
                     VVGAIQGRLETLNRYPDPDKTALRKALSDRTGVGADRITVGNGSCEILLSSADALLEP
                     GAELVYAWPSFSVYPMLASMSGATEIKVPLDDEGNHDLDAMAREITAATRLVLVCNPN
                     NPTSTALPLDRIAEFVAGVPDHVAVILDEAYVEFNLLQDPDESVELLAKHSNLILLRT
                     FSKIYGLCGLRVGYALGPERFRRAIDHVRQPFSVNALAQAAATEALRHQDEIARRIES
                     TVIERVHMETELDARGLGHPESQANFSWISLGDRDEKAVVDGLAEQGVIVRAGSALGE
                     EGRLRVTYGTRAENDRFLAALDTLL"
     gene            complement(3086..3451)
                     /gene="aroH"
                     /locus_tag="VFY44_13270"
     CDS             complement(3086..3451)
                     /gene="aroH"
                     /locus_tag="VFY44_13270"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX5911458.1"
                     /translation="MKLRALRGATTVAANQADAILEATDELVHEVIQRNGLRIEDMVS
                     CIFTCTEDLDAEFPAVAAREMGMSGVPLLCTREIPVPGSLPRVIRLLLHTYADEATEA
                     KHVYLREAVSLRRDLEGAQ"
     gene            complement(3448..4161)
                     /locus_tag="VFY44_13275"
     CDS             complement(3448..4161)
                     /locus_tag="VFY44_13275"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX5911459.1"
                     /translation="MRLAKYLAHSGVASRRAAERLIAAGTVTVGGEVVTDPARDVDES
                     SGVRVDGQAVAPEAHEVHLLNKPAGVVSTASDTHGRRTVVEYVASRRRLYPVGRLDVD
                     TTGLILLTNDGALADRLTHPRYGVQKTYRASVRPADIPDDAVSRLKAGVTLDDGRTSP
                     AEVTVLGPGLMEITIGEGRKRQVRRMCEAVGHKVSSLERVRFGPLTLGGLPQGESRAL
                     TTAEVSELYAAAGWETSAP"
     gene            complement(4165..5496)
                     /locus_tag="VFY44_13280"
     CDS             complement(4165..5496)
                     /locus_tag="VFY44_13280"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HEX5911460.1"
                     /translation="MDLERLAAGGDGREERIVEVIGDAFAELIAADPEAFRRKFRKMA
                     ADPFAFYRGSAPLFYADVAGLDDPFVDERTSRVWIQGDLHPENYGTYMSADGLLVFDV
                     NDFDEAYLGHYTWDLLRMAAGVSLLGFTMALSDDTVRQVNRTYATAYAEQVRAFAEGD
                     KDEEFGLRLGNTEGTVQTVLRAAREQTRVRLLEAQTKIDGGERRFAERTGVRTLGADE
                     QGAVYAAFRDYLDTVPSDKRQQSVSYSVKDIVGRSGFGIGSAGLPAYSLLIEGRTEAL
                     QDDVVLSMKQGNVAAASRVVTDEAITDAFQHHGHRTVVSQRALQAHADPWLGWCELDG
                     TGFVVSEVSPYEEDLDWDLVASPDEIEPLVRSLGQATAKVHCVPDASSDQTLVDFDVE
                     KAITEVVGDRLDEFADHVADFGSAYAGLVREDHRRFVDAFRNGAIPGLPVD"
     gene            5520..6506
                     /gene="rlmN"
                     /locus_tag="VFY44_13285"
     CDS             5520..6506
                     /gene="rlmN"
                     /locus_tag="VFY44_13285"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX5911461.1"
                     /translation="MDLALLDTTLADLGQPAFRATQVWEWTARGAGSYEEMTNLPESL
                     RAQLAERVPLSSLTVEHEALASDGTEKVLLRTGDARPLEAVLMRFRDGRRSLCVSSQS
                     GCPLTCTFCATGTMKFGRNLTTSEILDQALHFRRKEDVDHLVFMGMGEPLMNLDNVLG
                     ACERLPDIGIHPSSTTISTVGWIPGIERLTERDGPTIRLALSLHAPDDDLRSELMPVN
                     DRYPLADVLAACLRWHEQRRRRVYIEYLMLDGVNDSPEQALQLARVLTPHEAFKVNLI
                     PYNPTDSGLTGSSREAIDTFVSTLHENGVPATVRLTRGRDIDAACGQLAVKA"
     gene            complement(6451..7374)
                     /locus_tag="VFY44_13290"
     CDS             complement(6451..7374)
                     /locus_tag="VFY44_13290"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5911462.1"
                     /translation="MSRRTGIEFAFVAAFWGASYLFIKVALEDVFSPPMIVFVRTALA
                     ALVLAPLAWRTGRVGALRGAGGVIALLAVIQVVAPFMLISYGEQHISSSLAGILVSTA
                     PIYAFLLAVVITPEERAKRIGAVGVAIGIAGVVLLLGVDAGGGTEALVGGAMVALAGL
                     GYAAGSFLIKRRIPGQAPIVLVTATMAVSAVFSLPFAATDLPGAAPSLEAVASLFALG
                     VLGTGIAFVFYYDLIDRLGPPKASLVAYIAPGFAVLYGVVLLGEDVSLATFAGLVLIL
                     LGSYLAAYERLPFVRPSPPADRKPRRYRAPA"
     gene            7446..8378
                     /locus_tag="VFY44_13295"
     CDS             7446..8378
                     /locus_tag="VFY44_13295"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5911463.1"
                     /translation="MLDVKRIKVFREVASCGSFSAAAESLSFTQSAVSQQVAALERET
                     GTQLLDRTPRGVRPTESGRALLAHADAILARLECAEDELAELAGLRGGRLRLAGFQSA
                     GATLLPRAVAMFHERHPKVELGMVEAEPAEAVERLRSGDIDLALVYDFEPLPGSLGPD
                     LELTHLLDDTYDAVIARSHPLADRKRLKLADLAQDPWIASTPRCGCRQITDRACLDAG
                     FESRVAFEADETMSAMALVAAGVGVTIFPRLALNPMHPGVVARPLGARAPVRRIWVAR
                     LAGGHRSAASEAMHQLLTDAAAEFRQDAPLQAVS"
BASE COUNT         1196 a         3058 c         2984 g         1160 t
ORIGIN      
        1 gtcgacgatc accggcaggt gcgtcaggtc cttgagcacc ggcaccgcca tcaggtccag
       61 cgtgaaccgg tacgcggtct cgaacgtgcg gatgccgcgc tcgcacagca ccacgtcgcc
      121 gttgccctcc ttgagcacgt actcggcggc catgaggagc tcctcgagcg tggcgctcag
      181 cccgcgcttg atcagcaccg gcacgccggc ccgcccgatc tcggtcagca gcgtgtagtt
      241 ctgcatgttg cgcgcgccca gctggatcat gtccgcgacc tcgcacacgg cgtccaggtc
      301 gcgcacgtcc atgacctcgg tgacgatggg catgccggtg gcctgcttgg cctcggccag
      361 cagcttcagg ccctcctggc ccagcccctg gaagctgtag ggcgaggtgc ggggcttgta
      421 cgcgccgccg cgcaacagct gggccccggc ggccttgacc acgtgcgcgg agtcgagcat
      481 cacgtcacgg gactcgaccg tgcaggggcc ggcgatggtg gcgaagtggt cgcccccgat
      541 cttgcgcccc gcgatgtcga tgaccgtccg cttgccgtgc gtgacctggc tcgaggcgag
      601 cttgaacggg cggctgatgg gcatcgtgtg gtctacgccg ggcgcgacct ccagggccag
      661 gtcgcgcacc agctcgacgt caccgatcac gccgatcacc tgctgcgcgg cgccctcgat
      721 cacgtgcgcc ttcgcgcccg tcgactccac ccgcgagacc acctcggaga cgtcctccgg
      781 tgtggcgtcc tctctcatca cgatcatcat ttcttcctgg aacctctcag attggcggtt
      841 gctgcgagcg ggggacggcg tccgcgtggg actggtgctg ccgtgaaggc ggacgcctag
      901 ctaaatcgcc aataggccgc gaaggcacgc caggaggtgc tgcgagagat ggcgtgcgcg
      961 cggctcacag gcctatatct agcagcccgg gtacaccggg gcaatgatcg ctttcctggg
     1021 aaccggaatc atgggcgcgc cgatggcgcg caacaccgcc gccgccggcc acgacgtgcg
     1081 ggtgtggaac cgcacccgcg ccaaggccga ggcggtcgat ggcgtccagg tggccggctc
     1141 gcccgaggag gcggtggcgg gcgcggacgt ggtcgtgacc gtcctggccg acggcgacgc
     1201 cgtgaccgcg gccatggagg accgcggcgc gctggccgcc atggccgacg acgcgctctg
     1261 gctgcagatg tccacggtgg gcatcgccgc caccgaggcg ctggccgccc tggccgagga
     1321 gcgcagcgtg ccgttcgtgg acgcgccggt gctgggcacg aaggcgccgg ccgagaaggg
     1381 cgccctgacc gtgctggcct cggggcccgg cgacgtgcac gagcgctgcg cgccggtctt
     1441 cgacgccgtc ggcgcgaaga ccgtgtggct gggcgaggcg ggcaccgcca cgcgggccaa
     1501 gctcgtgctc aaccactggg tgctctcgct gacccaggcc gtggccgaga cggtgggcct
     1561 ggccgaggcg ctggacatcg acccccagct cttcctggac tccatcgagg gcgggcccct
     1621 ggacagcggc tacgcccaga tgaagggcgg cgcgatcatc gcccgctcgt tcgaccccag
     1681 cttcacgctc tcgctggccc gcaaggacgc cgggctggtg ctggaggccg ccgagcgcca
     1741 cgagcacgag gcagccctcg ccgaggtggt cgagcgccag atgaccaagg cgatcgaggc
     1801 cggccacggc gacgaggaca tggcggcaac gtacttcgcc tcccggccgc agtaggcgcc
     1861 tgaatcgcgt cccgacttcc gtcgcgaata cagacggaac tcgtgacgct ggccagaccg
     1921 ggtaacggct cggtcacggg cacaacggca gcgttacaag ccccccgccc tacccagccc
     1981 agcagttggc cgggcccgca ggggacgcct cagaggagcg tgtccagcgc cgccagaaag
     2041 cggtcgttct ccgcccgcgt gccgtaggtc acgcgcaggc ggccctcctc gcccagcgcg
     2101 ctgccggcgc ggacgatcac gccctgctcg gccagcccgt cgaccacggc cttctcgtcg
     2161 cggtcgccca gcgagatcca cgagaagttg gcctggctct cggggtggcc gaggccgcgg
     2221 gcgtccagct cggtctccat gtgcacccgc tcgatgaccg tggactcgat ccggcgggcg
     2281 atctcgtcct ggtggcgcag cgcctcggtg gcggcggcct gcgccagggc gttgaccgag
     2341 aacggctggc gcacgtggtc gatggcgcgg cggaagcgct cgggccccag ggcgtagccc
     2401 acccgcagcc cgcacaggcc gtagatcttg gagaaggtgc gcagcaggat caggttggag
     2461 tgcttcgcga gcagctcgac cgactcgtcc gggtcctgga gcaggttgaa ctccacgtag
     2521 gcctcgtcca ggatcaccgc gacgtggtcg ggcacgccgg ccacgaactc cgcgatccgg
     2581 tccagcggca gcgccgtgga ggtcgggttg ttggggttgc acaccagcac cagccgggtg
     2641 gccgcggtga tctcgcgcgc catcgcgtcc aggtcgtggt tgccctcgtc gtccagcggc
     2701 accttgatct cggtggcgcc cgacatcgag gccagcatcg ggtagaccga gaacgagggc
     2761 caggcgtaca ccagctcggc gccgggctcc agcagcgcgt cggcggacga cagcaggatc
     2821 tcgcacgagc cgttgcccac cgtgatccgg tcggccccca ccccggtccg gtccgacagc
     2881 gccttgcgca gcgccgtctt gtcggggtcg ggatagcggt tgagcgtctc gagccggccc
     2941 tggatggccc ccaccaccgc ggcgggcggg ccgaacgggc tctcgttgga ggccagcttg
     3001 accagctcgc cgccgaagtc gtagctgccg gcctgcgggt aggacgggat gtcgcccaga
     3061 cggcggttga actcgatccc ggggctcact gtgccccctc caggtcgcgc cgcagcgaca
     3121 cggcctcgcg caggtacacg tgcttggcct cggtggcctc gtcggcgtag gtgtgcagca
     3181 gcagccggat cacgcgcggc agcgagcccg gcaccgggat ctcgcgcgtg cacagcagcg
     3241 ggacgcccga catgcccatc tcgcgcgcgg cgaccgccgg gaactcggcg tccaggtcct
     3301 cggtgcaggt gaagatgcag ctgaccatgt cctcgatccg caggccgttg cgctggatga
     3361 cctcgtgcac cagctcgtcg gtggcttcga ggatggcatc ggcctggttc gcggcgaccg
     3421 tggtggcgcc tctgagtgcc ctgagcttca cggcgcggaa gtctcccagc ccgcggcggc
     3481 gtacagctcg gagacctcgg cggtggtcag cgcccgcgac tcgccctggg gcagcccgcc
     3541 cagcgtcagc ggcccgaagc gcacccgctc gagcgagctg accttgtgcc cgaccgcctc
     3601 gcacatgcgc cgcacctggc gcttgcgccc ctccccgatc gtgatctcca tgagcccggg
     3661 gccgagaacg gtgacctcgg ccgggctggt cctcccgtcg tccagcgtga cccccgcctt
     3721 caggcggctg acggcatcgt cggggatgtc cgccgggcgg acgctggccc tgtaggtctt
     3781 ctggaccccg tagcgcgggt gggtcaggcg atcggccagg gcgccgtcgt tggtcagcag
     3841 gatcagcccg gtggtgtcca cgtccaagcg ccccaccggg tacagccggc ggcgcgaggc
     3901 cacgtactcg accaccgtgc ggcggccgtg ggtgtcggac gccgtggaga ccacgccggc
     3961 cggcttgttc agcaggtgca cctcgtgcgc ctcgggggcc acggcctggc cgtccacgcg
     4021 cacgccgctg gactcgtcca cgtcgcgggc cgggtcggtc acgacctccc cgccgaccgt
     4081 cacggtgccc gccgcgatca gccgctcggc cgcgcgccgc gacgccacgc ccgagtgggc
     4141 caggtacttc gccaggcgca ccggtcagtc caccggcagg ccggggatgg ccccgttgcg
     4201 gaaggcgtcc acgaaccggc gatggtcctc gcgcaccagg ccggcgtagg ccgagccgaa
     4261 gtcggccacg tggtcggcga actcgtccag gcggtccccg accacctcgg tgatggcctt
     4321 ctccacgtcg aagtccacaa gcgtctggtc ggagctggcg tccggcacgc agtggacctt
     4381 ggccgtggcc tgcccgagcg agcgcaccag cggctcgatc tcgtccggcg aggccaccag
     4441 gtcccagtcc aggtcctcct cgtacggaga gacctcggag accacgaagc cggtgccgtc
     4501 cagctcgcac cagcccagcc acgggtcggc gtgggcctgc agcgcccgct gcgagaccac
     4561 ggtccggtgc ccgtggtgct ggaaggcgtc ggtgatcgcc tcgtcggtga ccacccggct
     4621 ggcggcggcc acgttgccct gcttcatcga cagcacgacg tcgtcctgca gcgcctccgt
     4681 ccggccctcg atcagcagcg agtaggcggg caggccggcg ctgccgatgc cgaagccact
     4741 gcggccgacg atgtccttga ccgagtagga cacgctctgc tggcgcttgt ccgacgggac
     4801 cgtgtccagg tagtcgcgga acgcggcgta gacggccccc tgctcgtcgg cgcccagcgt
     4861 ccggacgccg gtgcgctcgg cgaaccggcg ctcgccgccg tcgatcttgg tctgcgcctc
     4921 gagcaggcgc acccgggtct gctcgcgcgc ggcgcgcagc acggtctgga cggtgccctc
     4981 ggtgttgccc agcctgaggc cgaactcctc gtccttgtcc ccctcggcga acgcgcgcac
     5041 ctgctcggcg taggccgtgg cgtaggtgcg gttgacctgg cgcacggtgt cgtcggacag
     5101 cgccatcgtg aagcccagga ggctcacgcc cgcggccatc cgcagcagat cccaggtgta
     5161 gtggcccagg taggcctcgt cgaagtcgtt cacgtcgaac accagcaggc cgtccgcgct
     5221 catgtaggtg ccgtagttct cggggtgcag gtcgccctgg atccacacgc ggctggtgcg
     5281 ctcgtccacg aacgggtcgt ccaggccggc gacgtcggcg tagaacagcg gcgcgctgcc
     5341 ccggtagaag gcgaacgggt ccgccgccat cttgcggaac ttgcgcctga aggcctcggg
     5401 gtcggcggcg atcagctcgg cgaacgcgtc gccgatgact tcgacgatgc gctcctcgcg
     5461 gccgtcgccg cccgcggcga gtcgttcgag gtccattgca cggactctag gctggaggag
     5521 tggacctcgc gctcctggac accacgctgg ccgacctggg ccagcccgcc ttccgcgcca
     5581 cccaggtgtg ggagtggacc gcgcgcgggg ccggctccta cgaggagatg accaacctgc
     5641 ccgagtcgct gcgcgctcag ctggccgagc gggtgccgct gtcctcgctg acggtggagc
     5701 acgaggcgct ggcctccgac ggcaccgaga aggtgctgct gcggaccggc gatgcccgcc
     5761 cgctggaggc ggtgctgatg cggttcaggg acggccgccg gtccctgtgc gtgtcgtcgc
     5821 agtcgggctg cccgctgacc tgcaccttct gcgccaccgg cacgatgaag ttcggccgca
     5881 acctgacgac ctccgagatc ctggaccagg cgctgcactt ccgccgcaag gaggacgtgg
     5941 accacctggt gttcatgggc atgggcgagc cgctcatgaa cctggacaac gtgctcgggg
     6001 cgtgcgagcg gctgccggac atcggcatcc acccctccag caccacgatc tcgaccgtgg
     6061 gctggatccc cgggatcgag cggctgaccg agcgcgacgg gcccacgatc cgcctggcgc
     6121 tgtcgctaca cgcccccgac gacgacctcc gcagcgagct gatgcccgtg aacgaccgct
     6181 acccgctggc cgacgtgctg gccgcgtgcc tgcgctggca cgagcagcgg cgccggcggg
     6241 tgtacatcga gtacctgatg ctcgacggcg tgaacgacag ccccgagcag gcgttgcagc
     6301 tggcgcgcgt gctgacgccg cacgaggcct tcaaggtgaa cctgatcccc tacaacccca
     6361 ccgactcggg tctgaccggg tccagccgcg aggccatcga cacgttcgtc agcacgctgc
     6421 acgagaacgg cgtgccggcc acggtgcggc tcacgcgggg gcgcgatatc gacgcggctt
     6481 gcggtcagct ggcggtgaag gcctgacgaa gggcagccgc tcgtaggcgg ccaggtagga
     6541 gcccagcagg atcagcacca ggccggcgaa ggtggccagg ctcacgtcct cgcccagcag
     6601 caccacgccg tagagcaccg cgaagcccgg cgcgatgtag gccaccagcg acgccttcgg
     6661 cggcccgagc cggtcgatca ggtcgtagta gaagacgaac gcgatgcccg tgcccagcac
     6721 ccccagcgcg aacagcgagg ccacggcctc gagactgggg gcggcgccgg gcaggtcggt
     6781 ggcggcgaag ggcagcgaga acaccgcgct gacggccatc gtggcggtga ccaggacgat
     6841 cggcgcctgg ccggggatcc ggcgcttgat caggaacgag cccgcggcgt agcccaggcc
     6901 ggccagcgcg accatggcgc cgcccaccag cgcctcggtg ccgccgccgg catcgacccc
     6961 caggagcagg acgacgccgg cgatgccgat ggccacgccc accgccccga tgcgcttggc
     7021 gcgctcctcg ggggtgatga ccacggccag caggaaggcg tagatgggag ccgtggacac
     7081 caggatcccg gccagcgagg aggagatgtg ctgctcgccg taggagatga gcatgaacgg
     7141 ggccaccacc tggatcacgg ccagcagggc gatcaccccg cccgcgccgc gcagggcgcc
     7201 cacacggccg gtgcgccagg ccagcggggc cagcaccagc gcggccagcg cggtgcgcac
     7261 gaagacgatc atcggcggcg agaacacgtc ttccagcgca accttgatga acaggtagga
     7321 agcgccccag aacgccgcga cgaacgcgaa ttcgatgccc gttcggcggc tcatgccgcc
     7381 gagcatgcca cgcgtgccgc atcacttcca attacgagtg cgcgggggta ccatcagccc
     7441 agctaatgct cgacgtcaag cgcatcaagg tcttccgtga ggtcgcgtcg tgcggctcgt
     7501 tctccgccgc ggcggagtcg ctgtcgttca cccagtcggc cgtcagccag caggtcgccg
     7561 ccctggagcg cgagaccggc acccagctcc tggaccgcac cccccgcggc gtgcgcccca
     7621 ccgagtcggg ccgcgcgctc ctggcccacg cggacgccat cctggcccgg ctggagtgcg
     7681 ccgaggacga gctggccgag ctggccggcc tgcgcggcgg gcggctgcgc ctggcgggct
     7741 tccagagcgc gggcgccacc ctgctgcccc gcgccgtggc gatgttccac gagcggcacc
     7801 ccaaggtcga gctgggcatg gtcgaggccg agcccgccga ggccgtcgag cgcctgcgca
     7861 gcggcgacat cgacctggcg ctggtctacg acttcgagcc gctgccgggg tcgctgggcc
     7921 cggacctcga gctcacccac ctgctggacg acacctacga cgccgtcatc gcccgctccc
     7981 accccctggc cgaccgcaag cggctcaagc tcgccgacct ggcccaggac ccgtggatcg
     8041 cctccacccc gcgctgcggc tgccgtcaga tcaccgaccg cgcctgcctg gacgccggct
     8101 tcgagtcgcg cgtggccttc gaggccgacg agaccatgtc cgccatggcg ctggtcgcgg
     8161 cgggcgtggg cgtgaccatc ttcccccgcc tggcgctgaa cccgatgcac ccgggcgtgg
     8221 tcgcacgccc gctgggcgcg agggccccgg tgcgccggat ctgggtcgcg cggctggccg
     8281 gcggccaccg ctccgcggcc agcgaggcga tgcaccagct cctgaccgac gcggccgccg
     8341 aattccggca ggacgcgccg ctgcaggccg tgtcctagcg gctcaggctt cctgggcc
//
LOCUS       DASUPW010000588         6425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_829885, whole genome shotgun sequence.
ACCESSION   DASUPW010000588 DASUPW010000000
VERSION     DASUPW010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6425
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829885"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..273)
                     /locus_tag="VFY44_13300"
     CDS             complement(52..273)
                     /locus_tag="VFY44_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 4"
                     /protein_id="HEX5911464.1"
                     /translation="MADEDQIPPAGEPIHLPGPSYLPVLVALGATIALVGVVLNWVVF
                     GIGVAIMVVAILRWVSQVRQEMDELPLEH"
     gene            419..1396
                     /locus_tag="VFY44_13305"
     CDS             419..1396
                     /locus_tag="VFY44_13305"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX5911465.1"
                     /translation="MASVLGIDVGGTKVAVARVEGARAEGKVEQATDLEHADALLDGI
                     EEAVGQVVGDGQGPEAIGVGVPSQVEFATGTALASVNIPLEGVPLREELSKRFGVPVF
                     VDNDANCAALAEAQMVTDPPAQHLMMLTLGTGVGGGVIVDGRIFRGHTGLGAELGHVI
                     VDGQTALAASAGGFPRPGSLEWHCSGRGLERAITEAARMAGDGPLSRKLDDDGRVSGR
                     EAVAAAQEGDATALEVFARFSRWLGLGIATFINTFEPECVVLGGGLSRAADLFLDGAK
                     AEAERNTLPALWERVRIDLARGGADAGVIGAGVLAAQELERLDQPPRSG"
     gene            1406..2281
                     /locus_tag="VFY44_13310"
     CDS             1406..2281
                     /locus_tag="VFY44_13310"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5911466.1"
                     /translation="MERVVTSNEEAIEAWDGVLFDRFVEFRDVLTTGLGVQGAAAMEL
                     VQPRPGDRVLDIGCGFGDASQQLAALVGPGGSVHGVDASARFVETARREAADAGVGNV
                     TFEVADVEATGFPSEYDLAFSRMGTMFFANPVAALRHVHGALVPGGRLCMVVWRQKAE
                     NDWMYRAEQAVERYVEEDEDSDELTCGPGPFSMANADTTSGILKAAGFEQISLRRSDL
                     PYRIGADLDDAVALALAIGPAGEVMRLAGDDAVRLRPEIEAAVAEQLAPLERPEGVMA
                     TSSTWIVTASKPVGV"
     gene            2355..4376
                     /gene="tkt"
                     /locus_tag="VFY44_13315"
     CDS             2355..4376
                     /gene="tkt"
                     /locus_tag="VFY44_13315"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX5911467.1"
                     /translation="MTLTKDETEVDALAIDTIRTLSMDGVQKANSGHPGAPMALAPVA
                     YQLFAKTMKHNPADPDWFDRDRFVLSAGHASMLLYSILHLTGYGVSMDDLKNFRQILS
                     PTAGHPEYEAEGTPGIEITTGPLGQGVGNAVGFALAERLLAARFNRDGHEVIDHHTYA
                     IASDGDMQEGVASEASSLAGHLELGKLTVFYDNNQIQLAGPTSMGFSEKVADRYEAYN
                     WHVQDLGEDLSTRALAEAIEAAHADDRPSLIIVQSHIGFGSPNKQDSQKAHGSPLGED
                     EVRLTKEAYGWDPDKSFYVPDEAAEAYAEVPERGKQLQAEWEERLSAFREAHGDLAAE
                     LDQFITGDLPDGWDADLPRFSPDDDDIATRKASEQVIQWAAKALPTLVSGSADLEPST
                     NTEIEDGGSVTKDDFSGRNVHYGVREHAMGAIVNALNRHGVRAFGSTFFNFLDYMKGS
                     VRLAALMELGVIQVFTHDSIGLGEDGPTHQPVEQLAHLRATPNINAVRPADANETALA
                     WQFALKSTEGPTALVLSRQGLPILDPDSIPADAVEKGAYVLRDGDDVILIATGSEVKI
                     ALEAAEALSEKDGLSARVVSMPCWDRFAEADQSYRDQVLPPAIRARVSIEAAATLGWE
                     KWVGEAGESLGMTGFGASGPAGELYEHFGLTSEHLAEAARRVHRSIQEA"
     gene            4378..5478
                     /gene="tal"
                     /locus_tag="VFY44_13320"
     CDS             4378..5478
                     /gene="tal"
                     /locus_tag="VFY44_13320"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEX5911468.1"
                     /translation="MAQNERLAALTNAGVAVWLDQIHREMIESGDLKALVDEKSLRGE
                     TSNPAIFEKAIMGGDAYDDKIEKFAGEGLDARETYLKIAIEDVTAACDVMRPVYDELD
                     GYDGYVSLEVEPAVAHDTDATAKQARELWEAVDRPNVMIKIPGTTEGVQAIEDATADG
                     INVNVTLLFSVESYENIAEAYIRGLERRKEAGESLDIHSVASFFVSRVDTEVDKRLEA
                     NGREDLIGKAAIANAQAAYQSFKRIFHGERFAELKAAGAPVQRPLWASTGVKDERYDD
                     TKYVAGLIAPETVNTMPLDTLEACAEKLEVEEGTADQDPTATLKELADAGIDMEDVTK
                     TLLDEGIDKFVEPFDKLIAGIEEQMAKVGQGA"
     gene            5475..>6425
                     /locus_tag="VFY44_13325"
     CDS             5475..>6425
                     /locus_tag="VFY44_13325"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911469.1"
                     /translation="MSEDPVGQRMAAALDEGVVRRIWEHDEILWGGPGPEIGNRLGWL
                     DIADRRLEDVDDLVAFAEQAREDGFTHAALLGMGGSSLGPEVIRRSFGTLENGLELHV
                     LDSTDPGAVRALEAAIDLENTLFVVSSKSGGTVETLSHMKHFYELAGRNGDQFVAVTD
                     PGSGLVDIAAERGFRRVFENDPDIGGRYSVLSYFGLVPAALAGVDVAHMCRSAAEAAA
                     ECRDETDANPGLAIGVMLGTLALQGCNKATFVVSPRIAAFGLWVEQLIAESTGKQGRG
                     ILPVADEPLTRPDFYGEDRVFVYLRDADQTDGDLDGRVEDL"
BASE COUNT         1018 a         2196 c         2377 g          834 t
ORIGIN      
        1 ccgttcgcct gacccgccgg ggacgctcgc gggagaacgc cggcgagggc gctagtgctc
       61 gagcggcagt tcgtccatct cctgccggac ctgcgagacc cagcggagga tggcgacgac
      121 catgatggcc acgccgatgc cgaagaccac ccagttcagc accacgccca cgagggcgat
      181 cgtggccccg agggccacca gcacggggag gtaggacgga ccggggaggt ggatcggctc
      241 gccggccggc gggatctggt cctcgtcggc catcagtaga cgaagccgac gacccacgcg
      301 gcggcgaaca cggccagcgc gatcacgctg gcgatgaggg cggtgcggag gtttcggcgg
      361 gcgagcttgc ggtccacgac cacggttttc taccagacag ggcagaatgc ccctccggat
      421 ggcctctgtg ctcggaatcg acgttggcgg caccaaggtc gccgtggccc gtgtggaggg
      481 cgcccgcgcg gagggcaagg tcgagcaggc gaccgacctc gagcacgccg acgcgctgct
      541 cgacgggatc gaggaggcgg tcggtcaggt ggtgggcgac ggccagggtc ccgaggcgat
      601 cggggtgggc gtcccgtctc aggtcgagtt cgccaccggc accgcgctgg ccagcgtgaa
      661 catccccctc gagggggtcc cgctgcggga ggagctgagc aagcgcttcg gggtcccggt
      721 gttcgtggac aacgacgcca actgcgccgc gctggccgag gcccagatgg tcaccgaccc
      781 gccggcgcag cacctgatga tgctgacgct ggggaccggc gtgggcggcg gggtgatcgt
      841 cgacggccgg atcttccgcg gccacaccgg cctgggcgcg gagctgggcc acgtgatcgt
      901 cgacggccag acggcgctgg cggcctcggc cggcggcttc ccgcggcccg ggtcgctgga
      961 gtggcactgc agcgggcgcg ggctggagcg ggccatcacc gaggccgcgc ggatggccgg
     1021 cgacgggccg ctgtcgcgca agctcgacga cgacgggcgc gtctccggcc gcgaggcggt
     1081 ggccgccgcc caggagggcg acgccaccgc gctcgaggtc ttcgcccggt tctcgcgctg
     1141 gctgggcctg ggcatcgcca ccttcatcaa caccttcgag cccgagtgcg tcgtcctggg
     1201 agggggcctg tcgcgcgccg ccgacctgtt cctggacggc gcgaaggccg aggccgagcg
     1261 caacaccctg ccggcgctgt gggagcgggt ccggatcgac ctggcccgcg gcggcgcgga
     1321 cgccggcgtg atcggcgccg gggtgctggc cgcgcaggag ctcgaacgcc tggatcagcc
     1381 cccgcgttcc ggttagtgtc ttcacatgga gcgcgtggtc acttccaacg aagaggccat
     1441 cgaggcctgg gacggcgtcc tgttcgaccg cttcgtggag ttccgggacg tcctgacgac
     1501 cggcctcggt gtccagggcg ccgccgcgat ggagctggtc cagccgcgtc ccggcgaccg
     1561 cgtgctggac atcggctgcg ggttcggcga cgcgtcccag cagctggcag cgctggtcgg
     1621 tcccggcggc tcggtgcacg gggtggacgc cagcgcgcgc ttcgtggaga ccgcgcgccg
     1681 ggaggcggcc gacgccggcg tgggcaacgt gaccttcgag gtggcggacg tggaggccac
     1741 cggcttcccc tccgagtacg acctggcgtt ctcgcgcatg gggacgatgt tcttcgccaa
     1801 cccggtcgcg gcgctgcggc acgtgcacgg cgcgctggtg cccggcgggc ggctgtgcat
     1861 ggtcgtgtgg cgccagaagg ccgagaacga ctggatgtac cgcgcggagc aggccgtcga
     1921 gcgctacgtg gaggaggacg aggactccga cgagctcacg tgcggcccgg gcccgttctc
     1981 gatggccaac gccgacacca ccagcggcat cctgaaggcg gccggcttcg agcagatcag
     2041 cctccggcgc tcggatctgc cctaccggat cggcgccgac ctggacgacg cggtcgcgct
     2101 ggcgctggcc atcggcccgg ccggcgaggt catgcgcctg gccggcgacg acgccgtgcg
     2161 cctgcgcccg gagatcgagg cggcggtggc cgagcagctg gccccgctgg agcgccccga
     2221 gggcgtgatg gccacctcct cgacgtggat cgtgaccgct tcgaagcccg tcggcgtttg
     2281 acttcccggt cacgggtagt gacccccgtg ggtgatactg ccctgacgac gcgacaacga
     2341 ggcgaaggga cggcatgacg ctgacgaagg acgagaccga ggtcgacgcc ctggcgatcg
     2401 acaccatccg cacgctgtcc atggacgggg tgcagaaggc gaactcgggt catccgggag
     2461 cccccatggc gctcgcgccc gtggcgtacc agctcttcgc gaagacgatg aagcacaacc
     2521 cggccgaccc ggactggttc gaccgcgacc ggttcgtgct cagcgccggg cacgcctcga
     2581 tgctcctgta ctcgatcctg cacctgacgg gatacggggt ctcgatggac gacctcaaga
     2641 actttcgcca gatcctgagc cccaccgccg gccaccccga gtacgaggcc gagggcacgc
     2701 cgggcatcga gatcaccacc ggcccgctgg gccagggcgt gggcaacgcc gtcggcttcg
     2761 cgctggccga gcggctgctg gccgcccgct tcaaccgcga cgggcacgag gtcatcgacc
     2821 accacaccta cgccatcgcg tccgacggcg acatgcagga gggcgtggcg tccgaggcca
     2881 gctccctcgc gggccacctg gagctgggca agctcaccgt cttctacgac aacaaccaga
     2941 tccagctggc gggccccacg tcgatgggct tctcggagaa ggtcgccgac cgctacgagg
     3001 cctacaactg gcacgtccag gacctgggcg aggacctgag cacgcgcgcg ctggccgagg
     3061 ccatcgaggc cgcccacgcc gacgaccgcc cgtcgctgat catcgtgcag agccacatcg
     3121 gcttcggctc gccgaacaag caggactccc agaaggccca cggctcgccg ctgggcgagg
     3181 acgaggtcag gctgaccaag gaggcctacg gctgggatcc cgacaagagc ttctacgtgc
     3241 ccgacgaggc ggccgaggcc tacgccgagg tgcccgagcg cggcaagcag ctgcaggccg
     3301 agtgggagga gcgcctgtcc gcgttccgcg aggcccacgg cgacctggcg gccgagttgg
     3361 accagttcat cacgggcgac ctgcccgacg gctgggacgc cgacctgccc cggttctcgc
     3421 cggacgacga cgacatcgcc acccgcaagg cgtccgagca ggtgatccag tgggccgcca
     3481 aggcgctgcc gacgctggtc agcggctcgg ccgacctgga gccctcgacc aacaccgaga
     3541 tcgaggacgg cggctcggtg accaaggacg acttcagcgg gcgcaacgtg cactacggcg
     3601 tgcgcgagca cgccatgggc gccatcgtca acgcgctgaa ccggcacggg gtgcgcgcgt
     3661 tcggctccac gttcttcaac ttcctggact acatgaaggg cagcgtgcgg ctggcggccc
     3721 tgatggagct gggtgtgatc caggtgttca cccacgactc gatcggcctg ggcgaggacg
     3781 gaccgaccca ccagccggtg gagcagctgg cccacctgcg cgctacgccg aacatcaacg
     3841 cggtgcggcc cgccgacgcc aacgagacgg cgctggcgtg gcagttcgcc ctgaagtcca
     3901 cggagggccc gaccgccctg gtgctcagcc gccagggcct gcccatcctg gacccggact
     3961 ccattccggc cgacgcggtg gagaagggcg cgtacgtgct ccgcgacggg gacgacgtga
     4021 tcctgatcgc caccggctcc gaggtgaaga tcgccctgga ggccgccgag gcgctgtccg
     4081 agaaggacgg cctcagcgcc cgggtggtga gcatgccctg ctgggaccgg ttcgccgagg
     4141 ccgaccagtc ctaccgcgac caggtgctgc cgcccgcgat cagggcgcgc gtgtccatcg
     4201 aggccgcggc cacgctgggc tgggagaagt gggtcgggga ggccggcgag tcgctgggca
     4261 tgaccggctt cggcgcttcc ggcccggccg gcgagctcta cgagcacttc ggcctaacgt
     4321 cagaacacct agccgaggcg gcccgccggg tgcatcggtc gatccaggag gcatgacatg
     4381 gcgcagaacg agcgactggc agcactgacc aacgccggtg tggcggtgtg gctggaccag
     4441 atccaccgcg agatgatcga gtcgggcgac ctcaaggcgc tggtggacga gaagtccctg
     4501 cgcggcgaga cctcgaaccc cgcgatcttc gagaaggcga tcatgggcgg cgacgcctac
     4561 gacgacaaga tcgagaagtt cgcgggcgag ggcctggacg cgcgcgagac ctacctgaag
     4621 atcgcgatcg aggacgtgac cgcggcgtgt gacgtgatgc gcccggtcta cgacgagctc
     4681 gacggctacg acggctatgt gtcgctggag gtcgagcccg ccgtggccca cgacaccgac
     4741 gccaccgcca agcaggcgcg cgagctgtgg gaggccgtgg accgccccaa cgtgatgatc
     4801 aagatcccgg gcaccaccga gggcgtgcag gccatcgagg acgccaccgc cgacggcatc
     4861 aacgtgaacg tgacgctgct gttcagcgtc gagtcctacg agaacatcgc cgaggcctac
     4921 atccgcggcc tggagcggcg caaggaggcg ggggagagcc tggacatcca ctccgtggcc
     4981 tcgttcttcg tctcacgcgt ggacaccgag gtggacaagc gcctggaggc caacggcagg
     5041 gaggacctga tcggaaaggc ggccatcgcc aacgcccagg ccgcctacca gagcttcaag
     5101 cggatcttcc acggcgagcg gttcgcggag ctcaaggcgg ccggtgcccc ggtgcagcgc
     5161 ccgctgtggg cctccaccgg cgtcaaggac gagcgctacg acgacaccaa gtacgtggcc
     5221 gggctgatcg cgcccgagac ggtgaacacc atgccgctgg acacgctcga ggcgtgcgcc
     5281 gagaagctgg aggtcgagga gggcaccgcc gaccaagacc cgacggcgac gctcaaggag
     5341 ctggccgacg ccggcatcga catggaggac gtgaccaaga cgctgctgga cgaggggatc
     5401 gacaagttcg tcgagccgtt cgacaagctg atcgccggca tcgaggagca gatggccaag
     5461 gtggggcagg gcgcgtgagc gaagaccccg tcggccagcg catggccgcg gcgctcgacg
     5521 agggcgtcgt gcgccgcatc tgggagcacg acgagatcct ctggggaggt cccgggccgg
     5581 agatcggcaa ccggctgggc tggctggaca tcgccgaccg ccggctggag gacgtcgacg
     5641 acctggtggc cttcgccgag caggccaggg aagacggctt cacgcacgcg gcgctgctgg
     5701 gcatgggcgg gtcgagcctg ggcccggagg tcatccggcg ctccttcggc acgctcgaga
     5761 acgggctgga gctgcacgtg ctggactcca ccgacccggg cgccgtgcgc gcgctggagg
     5821 ccgcgatcga cctggagaac acgctgttcg tggtctcctc caagtccggc ggcacggtgg
     5881 agacgctgtc gcacatgaag cacttctacg agctggccgg gcgcaacggc gaccagttcg
     5941 tggccgtgac cgaccccggc agcggcctgg tggacatcgc cgccgagcgc gggttccggc
     6001 gcgtgttcga gaacgatccc gacatcggcg gccgctactc ggtgctgtcc tacttcgggc
     6061 tggtgccggc cgcgctggcc ggcgtcgacg tggcccacat gtgccgctcc gctgccgagg
     6121 cggccgccga gtgccgggac gagaccgacg ccaacccggg cctggccatc ggggtcatgc
     6181 tcggcacgct ggcgctgcag gggtgcaaca aggccacgtt cgtggtcagc ccgaggatcg
     6241 ccgcgttcgg cctgtgggtc gagcagctga tcgccgagtc caccggcaag cagggccgcg
     6301 gcatcctgcc ggtggccgac gagccgctga cgcggcccga cttctacggc gaggaccggg
     6361 tcttcgtcta cctgcgcgac gccgaccaga ccgacggcga cctggacgga cgggtcgagg
     6421 acctg
//
LOCUS       DASUPW010000589         2963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_968399, whole genome shotgun sequence.
ACCESSION   DASUPW010000589 DASUPW010000000
VERSION     DASUPW010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968399"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /locus_tag="VFY44_13330"
     CDS             <1..1323
                     /locus_tag="VFY44_13330"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX5911470.1"
                     /translation="HPVRARPRKGLRQVDTPRTVRNSLMRRAALVAGLAAVGTLTMAS
                     GAQAAQRYVADEVIVKYKSGITEAQRSGLLQSLDVERTIGAVKGVNARVLRVDGDAAA
                     VAKRLNASSSVLYAEPNYILRTQATPNDARFGELYGLNNTGHTGGTADADIDAPEGWD
                     AAGLGSFPATGGTKIGIVDTGIDKAHEDLSGKVTDCGGVKSFGLAGLIGGDPTIVADK
                     CTDDNDHGTHVAGTIAAKANNGVGVAGVSFNSPLSICKALSSGGSGSLAGVANCITYL
                     NQKGAKVISMSLGGSSGATTLQQAVQNAYKGGGAGGSLIIAAAGNDGNATLNYPAAYA
                     EVVSVAATDNRDARASFSNANSDVEVAAPGVDVLSTKRGGGYVAFSGTSMATPHAAGV
                     AGVIFTKNPGLTPGQVRSRLDAAVDDKGAAGRDSSFGFGRVNLAKAAQ"
     gene            1398..1868
                     /locus_tag="VFY44_13335"
     CDS             1398..1868
                     /locus_tag="VFY44_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911471.1"
                     /translation="MARTGRLTASSRLAAAADRVWAEATAAAGINYELMPIVRMTVPR
                     GLDTLSVGDVHDGQHVGRSWLLLGGVLPFDYDDITIERVGPGLAFQEDSTMLSQRSWR
                     HHRVVEPIGDRACTVTDTVEFVPRLPVPVRALELVFGQTFAHRHRRLRRRYGTA"
     gene            1868..2092
                     /locus_tag="VFY44_13340"
     CDS             1868..2092
                     /locus_tag="VFY44_13340"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX5911472.1"
                     /translation="MRITDARGLNCPMPLVMAKTAMETVPAGEELMVLATDPEAAIDL
                     AAWALDQGYELTERSAEGWTEYVLRSSSPA"
     gene            complement(2023..>2963)
                     /locus_tag="VFY44_13345"
     CDS             complement(2023..>2963)
                     /locus_tag="VFY44_13345"
                     /inference="COORDINATES: protein motif:HMM:NF018657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HEX5911473.1"
                     /translation="TRLAIGALSGGAAESLRAARGVARIAGTPRAGGARMYDTVRRAA
                     LAVGDDLLRPAPASYLNVPIGPKRTLVGHRARFDHVGEVRAATGTTVNDVCLAAVAGA
                     LRETALARGETPRALKAMVPVNVRTDDERHALGNRISMAFLDLPVGLSSPAGRLEAVR
                     RASADFKRSGKPEGTGTVFGALGMLPTPLRTGAARMVGSKRVYNLTVSNIPGPRAPLY
                     VLGARLREAYPVVPLYEEHALSIGIFGYRDHLHFGLYADPEVLPDVTAMPAALSAAML
                     ALRRTARRGGARTRYAGELLRRTYSVQPSAERSVSS"
BASE COUNT          435 a         1108 c         1061 g          359 t
ORIGIN      
        1 catcccgtgc gcgcgcggcc caggaagggc ctgcggcagg tcgatacccc aagaacagtg
       61 aggaacagcc tgatgcgtag agcagcgctc gtcgccggac tggcggcagt gggcaccctg
      121 accatggcct ccggggccca ggcggcccag cggtacgtgg ccgacgaggt catcgtcaag
      181 tacaagagcg gcatcaccga ggcccagcgc tccggtctgc tccagagcct ggacgtggag
      241 cgcaccatcg gcgccgtgaa gggcgtgaac gcccgcgtgc tgcgcgtgga cggcgacgcc
      301 gccgcggtgg ccaagcgcct gaacgccagc tccagcgtgc tctacgccga gcccaactac
      361 atccttcgca cccaggccac gccgaacgac gcccgcttcg gcgagctgta cggcctgaac
      421 aacaccggcc acaccggcgg caccgccgac gccgacatcg acgcccccga gggctgggac
      481 gccgcggggc tgggctcctt ccccgccacc ggcggcacca agatcggcat cgtggacacc
      541 ggcatcgaca aggcccacga ggacctgtcg ggcaaggtca ccgactgcgg tggcgtgaag
      601 tcgttcggcc tcgcggggct gatcggcggc gaccccacga tcgtggccga caagtgcacc
      661 gacgacaacg accacggcac gcacgtggcg ggcaccatcg ccgccaaggc caacaacggc
      721 gtcggcgtgg ccggcgtgtc gttcaactcc ccgctgtcca tctgcaaggc gctgagctct
      781 ggcggcagcg gctcgctggc gggcgtggcc aactgcatca cctacctgaa ccagaagggc
      841 gccaaggtca tctcgatgag cctgggcggc tcgtcgggcg cgaccacgct ccagcaggcg
      901 gtccagaacg cctacaaggg cggcggcgcg ggcggcagcc tgatcatcgc ggccgccggc
      961 aacgacggga acgccacgct gaactacccg gcggcctacg ccgaggtggt gtcggtggcg
     1021 gccacggaca accgtgacgc cagggccagc ttctccaacg ccaactcgga cgtggaggtc
     1081 gcggcgccgg gcgtggacgt cctgtccacg aagcgtggcg gcggctacgt ggccttcagc
     1141 ggcacgtcga tggccacccc ccacgccgcc ggcgtggccg gggtgatctt caccaagaac
     1201 ccgggcctca cgcccggcca ggtgcgctcg cgcctggatg ccgcggtgga tgacaagggc
     1261 gcggcgggcc gcgactcgag cttcggcttc ggtcgcgtga acctggcgaa ggccgcgcag
     1321 taggaacgac cccggtcggg acagggcagc acgacgggcg gccaccgggc cgcccgtcgt
     1381 cgttctaggg tccgggcatg gcccgcaccg gcaggctcac cgcgtcgtcg cgcctggcag
     1441 ccgccgccga tcgcgtgtgg gcggaggcca cggcggccgc ggggatcaac tacgagctca
     1501 tgcccatcgt gcgcatgacc gtgccccgcg ggctggacac gctctcggtc ggcgacgtgc
     1561 acgacggcca gcacgtgggc cgcagctggc tcctcctcgg cggggtcctg cccttcgact
     1621 acgacgacat caccatcgag cgggtcgggc cgggcctggc cttccaggag gactcgacca
     1681 tgctcagcca gcgctcgtgg cgccaccacc gggtggtcga gccgatcggc gaccgtgcct
     1741 gcaccgtgac cgacacggtc gagttcgtgc cacgcctgcc ggttcccgtc cgcgcgctgg
     1801 agctcgtctt cggccagacg ttcgcccacc gccaccggcg cctgcgccgc cgctacggga
     1861 ccgcctgatg cgcatcaccg acgcccgcgg gctgaactgc ccgatgccgc tggtcatggc
     1921 caagacggcc atggagaccg tgcccgccgg cgaggagctg atggtgctgg ccaccgaccc
     1981 cgaggccgcc atcgacctgg cggcgtgggc gctggaccag ggctacgagc tgaccgagcg
     2041 ctcggccgaa ggctggaccg agtacgtcct gcgcagcagc tcgcccgcgt agcgcgtgcg
     2101 cgcaccgccg cggcgggccg tgcgccgcag cgccagcatg gccgcgctga gcgcggccgg
     2161 catcgcggtc acgtcgggca gcacctcggg atcggcgtag agcccgaagt gcaggtggtc
     2221 gcggtagccg aagatcccga tcgacagcgc gtgctcctcg tacagcggca ccaccggata
     2281 ggcctcgcgc agccgggcgc ccagcacgta cagcggggcg cgcggcccgg ggatgttgga
     2341 gacggtcagg ttgtagacgc gcttggagcc caccatccgg gccgcgccgg tgcgcagcgg
     2401 ggtgggcagc atccccagcg cgccgaacac cgttccggtg ccctcgggct tgcccgaccg
     2461 cttgaagtcg gcgctggccc tgcgcaccgc ctcgagccgc ccggcggggc tggacagccc
     2521 gaccggcagg tccaggaagg ccatcgagat gcggttgccc agcgcgtgac gctcgtcgtc
     2581 ggtgcgcacg ttcacgggca ccatggcctt gagcgccctc ggcgtctcgc cgcgcgcgag
     2641 cgccgtctcg cgcagcgcgc ccgccaccgc cgccaggcag acgtcgttca cggtcgtgcc
     2701 ggtggcggcg cgaacctcgc ccacgtggtc gaagcgcgcc cggtggccca ccagcgtgcg
     2761 cttcggcccg atcggcacgt tgaggtacga cgcgggcgcc gggcgcagga ggtcgtctcc
     2821 gaccgccaat gccgcccggc gcaccgtgtc gtacatgcgc gccccacccg cgcgcggggt
     2881 gccggcgatc ctcgccaccc cgcgcgcggc gcgcagcgac tcggccgctc cccccgagag
     2941 agcgccgatc gcaagcctgg tcc
//
LOCUS       DASUPW010000590         2387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_553460, whole genome shotgun sequence.
ACCESSION   DASUPW010000590 DASUPW010000000
VERSION     DASUPW010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553460"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VFY44_13350"
     CDS             complement(<1..329)
                     /locus_tag="VFY44_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911474.1"
                     /translation="MARNRQKAKERQARRKAQKGNGDAKSAPPRRQGDDPIADDVLDE
                     LERESITEAGADPTVTPPLSGSERLRAPDAAEADAEPELGYPDIENDPEHFDPAEDQP
                     EDVDDLTP"
     gene            complement(345..509)
                     /gene="rpmG"
                     /locus_tag="VFY44_13355"
     CDS             complement(345..509)
                     /gene="rpmG"
                     /locus_tag="VFY44_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX5911475.1"
                     /translation="MARGDVRIAATLECQECKRRNYQTQKSKRNNPDRIELRKYCRWC
                     GKHTPHKETR"
     gene            complement(664..1416)
                     /locus_tag="VFY44_13360"
     CDS             complement(664..1416)
                     /locus_tag="VFY44_13360"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX5911476.1"
                     /translation="MANGAESGLTIDQLAQRTGMTVRNIRAHQSRGLLDPPDVRGRTG
                     YYGRAHVERIDLIRRLQADGYSLDLIGRMIVTAGDQSTEALRFTEALRRPWADEQPRV
                     VGGSELVGRYGTDDPELLAKAEELGLLRPLGGDQWEELSPRLGRAGAELTDLGIPAKE
                     ALRLVERVRRNADSAARSFVHLFVEEVWKPFQRDGSPEERWPEILDALERLRPLASDS
                     LMAVFLMAMEEEVEEALGRELKRARRDAKRRR"
     gene            1508..>2387
                     /locus_tag="VFY44_13365"
     CDS             1508..>2387
                     /locus_tag="VFY44_13365"
                     /inference="COORDINATES: protein motif:HMM:NF016915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911477.1"
                     /translation="MAATGFGQAASRIAEIHQAVADRSFGASGPGGRPAKLAHDAISA
                     GVYAAVRGVGGALMRAGGAAVSLRQAADAPALSETVAGTHALAALSGAFGDALEREGS
                     PLAVRMSVREDGREVPMDSEAVARAYPDATPRVAVFTHGLCESEAGWHLAAERHYGDA
                     DSWHGSRLRRDIGFTPVKIRMNTGLHISENGRRLAELLERLDGCWPVPVEEVLLVGHS
                     MGGLINRSACHYGMQDGHAWVGRVRNVVTLGAPHLGAPLEQAAARLGVALNWTPESRP
                     LARALAQRSVGIKDLRY"
BASE COUNT          290 a          863 c          872 g          362 t
ORIGIN      
        1 ggcgtcagat cgtcgacgtc ctcgggctgg tcctcggccg ggtcgaagtg ctccgggtcg
       61 ttctcgatgt ccgggtaccc gagctcgggc tcggcatcgg cctcggcggc gtccggcgcc
      121 cgcaggcgct cggatccgct cagcggcggc gtgaccgtgg ggtccgcgcc ggcctcggtg
      181 atggactcgc gctcgagctc gtcgagcacg tcgtccgcga tcgggtcgtc cccctggcgc
      241 cgcggcggag cgctcttcgc gtcgccgttg cccttctggg ccttgcggcg agcctggcgc
      301 tccttggcct tctgacgatt cctggccacg cggctgcgaa ccctctagcg ggtttccttg
      361 tggggggtgt gcttgccgca ccagcggcag tacttgcgca gctcgatccg gtccggattg
      421 ttgcgcttgg acttctgcgt ctgatagttg cgccgcttgc actcctggca ctccagggtg
      481 gcggcgattc gtacgtctcc gcgagccatg gctcctcgac tcctcggtgg gtaaacggcc
      541 gggggcaaac aaaaaccccg ccggccgcag ggtcaaacag tgaggatagc gcgctcagcg
      601 cgcagccggg tccccggcag ccgccgttca aggacagggc atggatgccc tgtccttggc
      661 gccctatctc cgccgtttcg cgtccctgcg ggcgcgcttg agttcgcgtc ccagcgcctc
      721 ctcgacctcc tcctccatgg ccatcaggaa cacggccatc agggagtccg aggccagcgg
      781 gcgcaggcgc tcgagcgcat ccaggatctc gggccagcgc tcctcggggc tgccgtcgcg
      841 ctggaacggc ttccacacct cctccacgaa caggtggacg aagctgcgcg cggcgctgtc
      901 ggcgttgcgg cgcacgcgct ccaccagccg cagcgcctcc ttcgcgggaa tgcccaggtc
      961 ggtgagctcg gcgccggcac ggcccagccg ggggctcagc tcctcccact ggtcgccgcc
     1021 cagcgggcgc aggaggccca gctcctccgc cttggccagc agctcgggat cgtcggtgcc
     1081 gtagcgcccc acgagctcgg atccgcccac cacccgcggc tgctcgtcgg cccacggccg
     1141 gcgcagtgcc tcggtgaagc gcagggcctc ggtgctctgg tcgccggcgg tgacgatcat
     1201 ccgcccgatc aggtccagcg agtacccgtc ggcctggagc cgccggatca ggtcgatcct
     1261 ctccacgtgc gcgcgcccgt agtagccggt ccgcccgcgc acgtcgggtg gatcgagcag
     1321 gccgcgcgac tggtgcgccc ggatgttgcg cacggtcatg cccgtccgct gggccagctg
     1381 gtcgatggtc aggccggact cggccccgtt cgccatgtga cgatcctagg ttgaagaggg
     1441 ccatgatccc gtgttacatg aactcatgca atcttctcgc gcagacgaag tgcgggcggt
     1501 tggaggtgtg gcggctaccg ggttcggcca ggccgcgagc cggatcgcgg agatccacca
     1561 ggcggtggcc gaccgctcgt tcggcgcctc gggccccggc ggccggccgg cgaagctggc
     1621 gcacgacgcc atatcggccg gcgtctacgc ggcggtcagg ggcgtcggcg gcgccctgat
     1681 gcgcgcgggc ggcgccgccg tctcgctgcg ccaggccgcg gacgcgcccg ccctgtccga
     1741 gacggtggcc ggcacccacg ccctggcggc cctgagcggc gccttcggcg acgcgctcga
     1801 acgcgagggc agccccctgg ccgtgcggat gagcgttcgc gaggacgggc gcgaggtgcc
     1861 catggacagc gaggccgtgg cccgcgcgta ccccgacgcg acgccgcggg tggccgtgtt
     1921 cacccacggg ctgtgcgaat ccgaggccgg ctggcacctg gccgccgagc gccactacgg
     1981 cgacgcggac agctggcacg gctcgcgcct gcgcagggac atcggcttca cccccgtgaa
     2041 gatccggatg aacacggggc tgcacatctc cgagaacggc cgccggctgg cggagctgct
     2101 ggagcgcctg gacggctgct ggccggtgcc ggtggaggag gtgctgctgg tggggcactc
     2161 gatgggtggc ctgatcaacc gcagcgcctg ccactacggc atgcaggacg gccacgcctg
     2221 ggtcggccgc gtgcgcaacg tcgtgacgct gggggcaccc cacctgggag cgccgctgga
     2281 gcaggccgcc gcccgcctgg gggtggcgct gaactggacg cccgagtcgc ggccgctggc
     2341 ccgcgcgctg gcccagcgca gcgtggggat caaggacctg cgctacg
//
LOCUS       DASUPW010000591         6489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_553707, whole genome shotgun sequence.
ACCESSION   DASUPW010000591 DASUPW010000000
VERSION     DASUPW010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6489
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553707"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VFY44_13370"
     CDS             <1..528
                     /locus_tag="VFY44_13370"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5911478.1"
                     /translation="RHDVVVLDRDLPEVHGDEVCRVISGELPATKVLMLTGSTGVNDL
                     VDGLGLGADDYMGKPFAFAELVARVRALGRRAGEARPAVLSAGDIELDPARHTAERAG
                     RELDLSPKEFGVLEILLGAGGATVSGEELLRAVWDEHADPFTNTVRMTMMKLRRKLGD
                     PPAIETVTGAGYRIE"
     gene            525..1571
                     /locus_tag="VFY44_13375"
     CDS             525..1571
                     /locus_tag="VFY44_13375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5911479.1"
                     /translation="MTVRARLTAAYALLFCATVGVLLGASYWLLSRHFDRTLSDAAAR
                     DALDAVGLQYLLAFGGTVLLALAAGWIIAGRALAPIGRMTAFARRVSGERLDERLALE
                     GPADELRELADTLDAMLDGLSESFGAQRRFVANAGHELRGPLTVIRTQAEVTLADPGA
                     SPAELRAMGEAVVEACKRTEALLEGLMALARSQRALVTREPTDLAPGARAAVAAVAEE
                     ARAADVRVRLEAGAAPTLGDRRLLDRLVANLVENAVRHNRPGGTVDVTTTTTNGHAAV
                     RVVNTGGQLADESVARLTEPFERLGRPADARGAGLGLSIVRSVAEAHRGEVRLAARDG
                     GGLEAEVLLPAEPA"
     gene            complement(1568..1780)
                     /locus_tag="VFY44_13380"
     CDS             complement(1568..1780)
                     /locus_tag="VFY44_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911480.1"
                     /translation="MRVKLALAAALVTGTALVGVGVGGFVGVDRQLEAATPKQGETRV
                     VGDHGDRRGDCPADADKPPAKQSRRL"
     gene            complement(1804..2760)
                     /locus_tag="VFY44_13385"
     CDS             complement(1804..2760)
                     /locus_tag="VFY44_13385"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5911481.1"
                     /translation="MSFGAPWFLLALIVVPLAAGAYLLLDRRRRRAAAAFASPATMPS
                     VVRSRPGWRRHAPMVVYALALIALALAFARPERTVAVPDGRAAVVLATDRSASMRATD
                     VRPSRITAVRRAAKGFLDDVPRQVKVGSVAFNDRVTRIESPRLQRGAVRESIDALAAE
                     GGTATGEAIAASMRSLERARGPRGKNPAAIILLSDGFSTSGRDPLEAAREARRKGIRV
                     YTVSLGTATGTIDVERRDGTVRQRQVPPDTASLREIARITKGRAYRAGDRIKLEEVYE
                     RLGDQVGTRKEKRQVTSAFAGGALLLLGAGGLMSLRFFGRLP"
     gene            complement(2757..3950)
                     /locus_tag="VFY44_13390"
     CDS             complement(2757..3950)
                     /locus_tag="VFY44_13390"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5911482.1"
                     /translation="MTPPSDLWAGGASERPRPRRPRRPGRTHLGLRPPEPPDGGHEPG
                     DDDRWKRWRAPIVAAAVASVLALSIGLVVGAGVFDGGDSDAVVVGGKAGQAGSAGKVY
                     AAAGPAVVSVRVRQGGRQASGTGFLVDGDGSIVTNSHVVGEAERVTVRFDDSGREIPA
                     RVRGTDPSSDLAVLDVDRSDVGSRRPLALARSSSVRVGDEVVAIGFPLGLDKTATEGI
                     VSGLERDIQAPNGFQIDRVIQTDAPINPGNSGGPLLDTRGRVVGVNSQIATAGAGASG
                     SIGIGFAVPADAVRNVLPRLKAGERIERPFIGLTSSADPRGGARVRDVTKGGPADQAG
                     LSAAGDVIVGIDGRPVRTPSDVSSAVSRLKPGDSVRVTVRRGGDRRTVRVRLTTRPER
                     LPGGP"
     gene            complement(4005..4766)
                     /locus_tag="VFY44_13395"
     CDS             complement(4005..4766)
                     /locus_tag="VFY44_13395"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5911483.1"
                     /translation="MSVLDRFRLDGKVALVTGASSGLGVAFAQALAEAGADVAVAARR
                     ADRLEQTRAQIEELGRGAIAVSCDVADPEQCANAVKETVDGLGRVDVLVNNAGIGTAV
                     PATRESADEFRKVIDINLNGSYWMAQACARVMEPGSAIVNIGSVLGSTTAGLPQAAYA
                     ASKAAVEGLTRDLASQWTSRKGIRVNALAPGFFPSEMTEQYPDGYLDMMMPRVLSGRK
                     GDPEELAAALLFLASDASGYVTGEVLHVDGGMVAT"
     gene            complement(4778..6010)
                     /locus_tag="VFY44_13400"
     CDS             complement(4778..6010)
                     /locus_tag="VFY44_13400"
                     /inference="COORDINATES: protein motif:HMM:NF022732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HEX5911484.1"
                     /translation="MIRFAPTAVLLALACALAPAAVATPAQAQTPPAKKKAKKPKLKS
                     KWLCGPRGPSSNPCQASQTATVLGRGGVNGGTRRGGLARNPRVDCFYVYPTVSPQPGP
                     NSNLRIGPEQRAAAQIQASRFSNSCRVYAPVYRQVTQAAFTNPAANTKAARARAYADV
                     RTAWREYLKYDNKGRGVVLIGHDQGAQMLTQLLAKEIERKTSSRDLLVSAILPGANVT
                     TLKGRRTGGSFRVVEPCRSNSQVGCFIAYSTFGEQPPPDAAVSRVYSPVFRRPPDRTK
                     RVACTNPSALVGGVGENKPYFPTRILPGPLSAMSPAIPVGTPWVYYPGLYTSRCTARQ
                     GATWLQITPTRNAADPRVRVQPVQGPQWGYHLADMNLFMAQLVTLVKIQAGQYPKVLR
                     ERARERAEEKREAESARR"
     gene            6072..>6489
                     /locus_tag="VFY44_13405"
     CDS             6072..>6489
                     /locus_tag="VFY44_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911485.1"
                     /translation="MLSALGYGLLASSSLVLGAGLALLRPWPPRAVGLVLAFGAGALI
                     SAVSFELAGEGLAVGSTSTVAIGLAGGALAYFVLDGLVSGEGRGRRGRKKAENPGTSL
                     ALGALLDGIPEQLVLGLGLAAGEGVGVGLLVAIYVSN"
BASE COUNT          778 a         2397 c         2493 g          821 t
ORIGIN      
        1 cgccacgacg tggtggtgct ggaccgcgac ctgccggagg tccacggcga cgaggtgtgc
       61 cgcgtgatct cgggcgagct gcccgcgacc aaggtcctga tgctcaccgg ctcgaccggg
      121 gtgaacgacc tggtcgacgg cctgggcctg ggcgccgacg actacatggg caagccgttc
      181 gcgttcgccg agctggtggc gcgcgtgcgc gcgctcggcc ggcgcgccgg cgaggcgcgc
      241 ccggcggtgc tcagcgcggg cgacatcgag ctggacccgg cacgccacac cgccgagcgg
      301 gcggggcgcg agctggacct gagccccaag gagttcggcg tgctggagat cctgctgggc
      361 gccggcggcg ccacggtgag cggcgaggag ctgctgcggg cggtgtggga cgagcacgcc
      421 gacccgttca ccaacacggt gcggatgacg atgatgaagc tgcgccgcaa gctgggcgac
      481 ccgccggcga tcgagaccgt gaccggcgcg gggtaccgga tcgaatgacc gtacgggcac
      541 gcctgacggc ggcatacgcg ctgctcttct gcgccaccgt cggggtgctg ctgggcgcca
      601 gctactggct cctctcgcgc cacttcgacc gcacgctgtc cgacgccgcc gcccgcgacg
      661 cgctggacgc ggtgggcctg cagtacctgc tggccttcgg cgggacggtc ctgctggcgc
      721 tggccgccgg ctggatcatc gccgggcgcg cgctggcgcc gatcggccgg atgacggcct
      781 tcgcgcgccg ggtgtccggc gagcgcctgg acgagcggct ggccctggag gggccggcgg
      841 acgagctgcg cgagctggcc gacacgctcg acgccatgct cgacgggctg tcggagtcct
      901 tcggcgccca gcgccggttc gtggccaacg ccggccacga gctgcgcggg ccgctcacgg
      961 tcatccggac ccaggccgag gtcaccctgg ccgaccccgg cgcgtccccc gccgagctgc
     1021 gcgccatggg cgaggccgtg gtcgaggcgt gcaagcgcac cgaggccctg ctcgaggggc
     1081 tgatggcgct ggcccgcagc cagcgcgccc tggtcacgcg cgagcccacc gacctggcgc
     1141 ccggcgcccg cgccgcggtg gccgccgtgg ccgaggaggc ccgtgccgcc gacgtccgcg
     1201 tgcgtctcga ggccggcgcc gccccgacgc tgggcgaccg gcggctgctg gaccggctgg
     1261 tggccaacct ggtcgagaac gccgtgcgcc acaaccgccc cggcggcacc gtcgacgtga
     1321 ccacgaccac cacgaacggg cacgccgcgg tgcgggtcgt gaacaccggc gggcagctgg
     1381 cggacgagtc cgtggccagg ctgaccgagc cgttcgagcg cctcggccgc ccggcggacg
     1441 cgcgtggcgc ggggctgggg ctgtcgatcg tgcgctcggt ggccgaggcc caccgcggcg
     1501 aggtcaggct ggcggcccgc gacggcggcg gcctggaggc cgaggtgctg ctgccggccg
     1561 agccggccta gagccggcgg gactgcttgg cgggcggctt gtcggcgtcg gccgggcaat
     1621 cgccgcggcg gtcgccgtgg tcgccgacca cgcgcgtctc gccctgcttc ggcgtcgcgg
     1681 cctccagctg gcggtcgacg cccacgaatc ctcctacgcc cacgcccacg agcgcggtgc
     1741 cggtgaccag cgcggccgcc agagcgagct tgaccctcat gcccctgacc atagccagcc
     1801 ccgtcagggc agcctgccga agaagcgcag cgacatcagc ccgcccgccc cgagcaggag
     1861 cagcgcgccg cccgcgaacg ccgaggtgac ctggcgcttc tccttgcgcg tgcccacctg
     1921 gtcgccgagc cgctcgtaga cctcctcgag cttgatccgg tccccggcgc ggtacgcgcg
     1981 gcccttggtg atgcgcgcga tctcgcgcag cgatgcggtg tcgggcggga cctggcgctg
     2041 gcgcacggtg ccgtcgcggc gctcgacgtc gatcgtgccc gtggccgtcc cgagcgacac
     2101 cgtgtacacg cggatgccct tgcggcgggc ctcgcgtgcc gcctccagcg ggtcgcggcc
     2161 gctggtggag aagccgtcgg agagcaggat gatcgcggcc gggttcttgc cgcgcggccc
     2221 gcgcgcccgc tccagcgagc gcatcgacgc ggcgatggcc tcgccggtgg cggtgccgcc
     2281 ctcggcggcc agcgcgtcga tggactcgcg cacggcgccg cgctggagcc gggggctctc
     2341 gatccgcgtg acgcggtcgt tgaaggccac gctgcccacc ttgacctggc gcggcacgtc
     2401 gtccaggaag cccttcgccg cccggcgcac cgcggtgatg cggctgggcc tgacgtcggt
     2461 cgcacgcatg gaggccgagc ggtccgtggc caggaccacc gcagcgcgcc cgtccggcac
     2521 agcgacggtg cgctcgggcc gggcgaaggc cagcgccagc gcgatcagcg ccagcgcgta
     2581 gaccaccatg ggcgcatgcc ggcgccagcc ggggcggctg cgcaccacgg acggcatcgt
     2641 ggccggcgac gcgaacgcgg ccgcggccct gcgccggcgg cggtccagca acaggtaggc
     2701 gccggcggcc agcggcacca cgatcagcgc cagcaggaac cagggcgcgc cgaagctcac
     2761 gggccgccgg gcagccgctc gggccgcgtg gtcaggcgca cgcgcaccgt gcgccggtcg
     2821 cccccgcgcc ggaccgtgac ccgcaccgag tcgcccggct tgagccgcga taccgcggac
     2881 gacacgtcgg acggggtgcg caccgggcgg ccgtcgatgc ccacgatcac gtcgcccgcc
     2941 gcgctcaggc cggcctggtc ggccgggccg cccttggtca cgtcgcgcac gcgagcgcca
     3001 ccgcgcgggt ccgccgagct ggtcaggccg atgaacgggc gctcgatccg ctcgcccgcc
     3061 ttcagccgcg gcagcacgtt gcgcacggcg tcggcgggca ccgcgaagcc gatgccgatg
     3121 ctgccgctgg cccccgcgcc ggccgtggcg atctgcgagt tcacgccgac cacgcgcccg
     3181 cgcgtgtcca gcagcgggcc gccggagttg ccggggttga tgggcgcgtc ggtctggatc
     3241 acgcggtcga tctggaagcc gttgggagcc tggatgtcgc gctccaggcc ggagacgatg
     3301 ccctcggtcg cggtcttgtc caggcccagc gggaagccga tggccaccac ctcgtcaccg
     3361 acgcgcaccg aggacgaccg ggccagggcc agcggccggc gcgagcccac gtcggagcgg
     3421 tccacgtcca gcaccgccag gtcggaggag gggtcggtgc cgcgcacccg cgcggggatc
     3481 tcgcgccccg agtcgtcgaa gcgcacggtc acgcgctcgg cctcgccgac cacgtgcgag
     3541 ttcgtgacga tcgagccgtc gccgtccacc aggaagccgg tgccggaggc ctgccggccc
     3601 ccctggcgca cgcgaaccga gaccacggcg ggcccggccg ccgcgtagac cttgcctgcg
     3661 ctgccggcct ggccggcctt gccgcccacc acgaccgcgt cggagtcgcc gccgtcgaag
     3721 accccggcgc cgaccaccag gccgatcgac agcgccagca ccgacgcgac ggcggcggcc
     3781 acgatcggcg cgcgccagcg cttccagcgg tcgtcgtcgc cgggctcgtg tcccccgtcc
     3841 ggcggctcgg gcgggcgcag ccccagatgg gtgcggccag gccggcgcgg gcggcggggg
     3901 cgcgggcgct ccgacgcgcc gccggcccag aggtcggagg gtggggtcac gccctggaaa
     3961 ctaggcgccc aaccgtcaac tcttcgtaaa gagcgccgcg gcgctcaggt ggcgaccatg
     4021 ccgccgtcca cgtgcagcac ctcgccggtc acatagccgg aggcgtcgct ggccaggaac
     4081 agcagggcgg cggccagctc ctcggggtcg cccttccggc cggacaggac gcgcggcatc
     4141 atcatgtcca ggtagccgtc ggggtactgc tcggtcatct ccgaggggaa gaagccgggc
     4201 gccagcgcgt tcacgcggat gcccttgcgc gaggtccact gcgaggccag gtcgcgcgtc
     4261 agcccctcga ccgccgcctt gctggcggcg taggcggcct gcggcaggcc ggccgtcgtg
     4321 gaccccagca ccgagccgat gttcacgatc gccgagcccg gctccatgac ccgggcgcag
     4381 gcctgcgcca tccagtacga gccgttcagg ttgatgtcga tgaccttgcg gaactcgtcg
     4441 gcgctctcgc gcgtggccgg caccgcggtg ccgatgccgg cgttgttgac cagcacgtcg
     4501 acccggccca gcccgtcgac ggtctccttg accgcgttcg cgcactgctc ggggtcggcc
     4561 acgtcgcacg acacggcgat ggccccgcgg ccgagctcct cgatctgggc gcgggtctgc
     4621 tccaggcggt cggcgcgccg cgcggcgacg gccacgtcgg cgcccgcctc ggccagcgcc
     4681 tgcgcgaagg ccacgcccag tcctgacgag gcgccggtca ccagcgccac cttgccgtcc
     4741 agccggaatc gatcgagaac gctcatggcc ggcgagccta ccggcgcgcc gactccgcct
     4801 cgcgcttctc ctcggcccgc tcgcgcgcac gctcgcgcag gaccttcggg tactgcccgg
     4861 cctggatctt gaccagcgtg accagctggg ccatgaacag gttcatgtcg gccaggtggt
     4921 agccccactg cgggccctgc accggctgca cccggacacg cgggtcggcc gcgttgcggg
     4981 tgggcgtgat ctggagccag gtcgcgccct ggcgcgccgt gcagcgcgag gtgtacaggc
     5041 cggggtagta gacccacggc gtgcccaccg ggatggccgg ggacatggcc gacagcgggc
     5101 cgggcaggat ccgggtcggg aagtagggct tgttctcgcc cacgccgccc accagcgccg
     5161 acgggttcgt gcacgccacg cgcttggtgc gatccggcgg gcgcctgaac accgggctgt
     5221 acacgcgcga gacggcggcg tcgggcggcg gctgctcgcc gaaggtggag taggcgatga
     5281 agcacccgac ctgggagttc gagcggcacg gctcgaccac ccggaacgag ccgccggtgc
     5341 gccgtccctt cagcgtggtc acgttggcgc cgggcaggat cgccgagacg agcaggtcgc
     5401 gggacgaggt cttgcgctcg atctccttgg ccagcagctg ggtcagcatc tgggcgccct
     5461 ggtcgtggcc gatcagcacc accccgcggc ccttgttgtc gtacttgagg tactcgcgcc
     5521 aggcggtgcg cacgtcggcg tacgcgcgtg cccgggcggc cttggtgttg gcggccgggt
     5581 tggtgaaggc ggcctgggtg acctggcggt acaccggcgc gtagacccgg cacgagttcg
     5641 agaaccgcga ggcctggatc tgtgccgcgg cgcgctgctc ggggccgatc cggaggttcg
     5701 agttgggccc cggctgcggg ctgaccgtgg ggtacacgta gaagcagtcc acccgcgggt
     5761 tgcgggccag cccgccgcgc cgtgtgccgc cgttgacccc gccgcgaccc aggaccgttg
     5821 cggtctggga ggcctggcag gggttggagg agggcccgcg cgggccgcac agccacttcg
     5881 acttgagctt gggcttcttg gccttcttct tcgccggcgg cgtctgcgcc tgggcaggcg
     5941 tggccacggc ggcgggggcc agcgcgcagg ccagggccag gaggacggct gtgggggcga
     6001 atcggatcac ggcgtcgcag gctagtaaca cctccgggcg gcggcgattc ggtacctggg
     6061 acgattcgcg cgtgctctcg gcgctcggct acggcctgct ggcctcctcc tcgctcgtgc
     6121 tgggagcggg cctggccctg ctccgcccgt ggccgccccg ggcggtgggc ctggtcctgg
     6181 ccttcggcgc gggcgcgctg atcagcgccg tcagcttcga gctggccggc gagggcctgg
     6241 cggtgggcag cacctccacg gtggccatcg gcctggcggg cggggcgctg gcctacttcg
     6301 tgctcgacgg gctggtgtcg ggggagggcc gcggccgcag gggccgcaag aaggccgaga
     6361 acccggggac gtcgctggcg ctgggcgcgc tgctggacgg gatccccgag cagctcgtgc
     6421 tgggcctggg cctggccgcg ggcgagggcg tcggcgtcgg cctgctggtg gccatctacg
     6481 tctccaacc
//
LOCUS       DASUPW010000592         4191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U1665
            k141_830864, whole genome shotgun sequence.
ACCESSION   DASUPW010000592 DASUPW010000000
VERSION     DASUPW010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961801
            Sequence Read Archive: SRR25158512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:57:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830864"
                     /isolate="SMAG_U1665"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41083333 N 108.9980556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VFY44_13410"
     CDS             <1..809
                     /locus_tag="VFY44_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5911486.1"
                     /translation="YKHRGAVESPPISLRRPVSSTVALFRSRWWLLGIAVAMVSWGFH
                     VAALALAPISIVQTVIAGGLVLLGVVADRLFGFTVTRREWIGVILTAAGLAFLAATLE
                     GTADSAHNDWDIATLTAYVGGLSLLGLLAAALARDTTRGPTLLAASAGLIWGASDVSI
                     KALSKHTDQGLAVLGHPLALVILVLSLVGLAISARSLQVGKAVPVIAVTSAAANIATI
                     ASGPIVFAEPLPDDPGALLLRLLAFALVITAASMTPPPMAEPEARPAPIG"
     gene            819..1844
                     /locus_tag="VFY44_13415"
     CDS             819..1844
                     /locus_tag="VFY44_13415"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5911487.1"
                     /translation="MAAQSLTVAVTGPTGDLGIAIVDKLERARSVKKVVGMARRPFDP
                     AEQGWKKTEYRQGDVQDAGSVRDTVKDADVLIHLAFSLMGEGDRTRAINVDGSRTVFE
                     AAAEAGVQRICYASSVAAYGFHADNPLWLTEEVPARGTAEHFYSAQKAEVESVLGEVL
                     LRHQRTAAYVFRPCVVAGPRAQMLLEEMPYYRISGAVPDAVVGLLSSMPILKPVIPDP
                     GVRFQLVHEDDVASAFLAGVRGQGEPGAYNLAGRGTIKMSDVADELGWYSVPVPEPVV
                     GITAGIVDRLPMAPESVSWIHAVRQPVLMKIDHARDDLKWKPEHTARATLKQMVAARR
                     AGAPAAR"
     gene            1903..3060
                     /locus_tag="VFY44_13420"
     CDS             1903..3060
                     /locus_tag="VFY44_13420"
                     /inference="COORDINATES: protein motif:HMM:NF025697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4394 domain-containing protein"
                     /protein_id="HEX5911488.1"
                     /translation="MRSTRILTAAAVTATALAAPSAASAAEQLAAVTNSNKVLTFASD
                     SPGNLDTVYPLTGLASGEKIVGLDTRPATGQLYGLGKTGRIYVIDAASGATRSVAGPI
                     SPLPAGSTYGFNFNPTVDRIRITTDQRQNLRVNPDTGDTIVDTPLAYAAGQGGGVAPQ
                     VGGVAYTNPVPTATTTQLFDLEAARDALTTQNPANSGTLTTVGNGLGQAISGPSGFDI
                     STSNTGWASYKPGGQGSVSLFKLNLTGGTVANAASQPSIGTRAAADDTVAIATLGAVA
                     DDKTAPKMVADSPTSASRVNLSRGKSFLGTVSLNEAGRVQVDMRVGKTLVAQGKAEIY
                     TRAGSSRIRVKATTSGMNTLKRKYKTATFRFSARDRAGNLTKGSARRFTLR"
     gene            complement(3091..3507)
                     /locus_tag="VFY44_13425"
     CDS             complement(3091..3507)
                     /locus_tag="VFY44_13425"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX5911489.1"
                     /translation="MTYFDDGDEVYRYIGGLLTGLIDSPDLGDKLKRADTTVQFRYHD
                     PEAVITFQLIEGQDVRVDLGESDLEPEIVMTADADVAHLFWLGRVNVTVALARGQMKA
                     SGPVAKILKLVPLIKPVFPLYRDLLEREGRTDLLEV"
     gene            complement(3504..>4191)
                     /locus_tag="VFY44_13430"
     CDS             complement(3504..>4191)
                     /locus_tag="VFY44_13430"
                     /inference="COORDINATES: protein motif:HMM:NF018657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HEX5911490.1"
                     /translation="TWTRLPLDELKEIKRELGGTVNDVFLTVVSGALHRWLRTRGVVT
                     EGLELRSAVPVSIRATGAEGDLGNQITIMIGRLPTYVEDPVERHRLVVESMADLKESK
                     QAMGAEVIASVEDFAPPTIFAQASRLHFSTRMYNLLTTNVPGPQFPLYLLGREMAELI
                     PVAFLAPEQRLAIAMMSYNGSAALSLMGDYDTMPDLERLVEFAQEELHALGDAAHDPT
                     MAAASGRSAR"
BASE COUNT          614 a         1542 c         1451 g          584 t
ORIGIN      
        1 tctacaagca ccgcggcgcg gtcgagtcgc cgccgatctc gctgaggcgc cccgtgtcca
       61 gcacggtcgc gctgttccgc tcgcgctggt ggctgctggg catcgccgtg gccatggtct
      121 cgtggggctt ccacgtggcc gcgcttgcgc tggccccgat ctcgatcgtg cagacggtga
      181 tcgccggcgg cctggtgctg ctcggcgtgg tggccgaccg gctgttcggc ttcaccgtga
      241 cccggcgcga gtggatcggc gtgatcctga ccgccgccgg cctggccttc ctggccgcca
      301 cgctggaggg caccgccgac tccgcccaca acgactggga catcgcgacc ctgacggcgt
      361 acgtcggcgg cctgtcgctg ctgggcctgc tggccgccgc gctggcccgg gacaccaccc
      421 gcggccccac gctcctggcc gcctccgcgg gcctgatctg gggcgcgtcc gacgtgtcga
      481 tcaaggcgct gagcaagcac accgaccagg gcctggccgt gctgggccac ccgctggcgc
      541 tggtgatcct ggtcctgtcg ctggtgggcc tggccatctc ggcccgcagc ctccaggtgg
      601 gcaaggcggt gccggtgatc gcggtgacca gcgccgccgc caacatcgcc acgatcgcct
      661 ccgggccgat cgtgttcgcc gagccgctgc ccgacgaccc gggcgcgctc cttttgcgcc
      721 tgctggcctt cgcgctggtc atcaccgccg cctcgatgac gccgccgccg atggccgaac
      781 ccgaagcacg ccccgccccg atcgggtaga accgtcatgt ggcagcccag agcctgaccg
      841 tcgcggtgac aggccccacc ggggacctgg ggatcgcgat cgtcgacaag ctcgagcgcg
      901 cccgcagtgt gaagaaggtc gtgggcatgg cgcggcgccc gttcgacccc gccgagcagg
      961 gctggaagaa gaccgagtac cgccagggtg acgtgcagga cgcgggcagc gtgcgcgaca
     1021 cggtcaagga cgccgacgtg ctgatccacc tggcgttctc gctgatgggt gagggcgacc
     1081 gcacccgcgc gatcaacgtg gacgggtcac ggacggtgtt cgaggcggca gcggaggccg
     1141 gggtgcagcg catctgttac gcctcaagcg tcgcggccta cggcttccac gccgacaacc
     1201 cgctgtggct gaccgaggag gtgcccgcgc ggggcaccgc cgagcacttc tactcggccc
     1261 agaaggccga ggtggagtcg gtcctcggcg aggtgctgct gcgccaccag cggaccgccg
     1321 cctacgtgtt ccggccgtgc gtggtggcgg ggccgcgcgc ccagatgctg ctggaggaga
     1381 tgccctacta ccggatctcc ggcgcggtgc cggacgccgt ggtcggcctg ctgtcctcga
     1441 tgcccatcct caagcccgtg atccccgacc ccggcgtgcg cttccagctg gtgcacgagg
     1501 acgacgtggc cagcgccttc ctggccggcg tccgcggcca gggcgagccc ggggcctaca
     1561 acctggccgg ccggggcacg atcaagatgt ccgacgtggc cgacgagctg ggctggtact
     1621 cggtcccggt gcccgagccc gtggtcggta tcaccgccgg gatcgtcgac cggctgccca
     1681 tggcgcccga gtcggtgtcg tggatccacg ccgtccgcca gcccgtgctt atgaagatcg
     1741 accacgcccg cgacgacctg aagtggaagc ccgagcacac ggccagggcc acgctgaagc
     1801 agatggtggc cgcgcgacgc gccggcgcgc cggcggcccg ctgagcttca caagatccgt
     1861 cccacaccga tccgatcgcc cgtccgaggc gttacgggtc atatgcgatc tacacgaatc
     1921 ctgacggcag ccgccgtcac agcgaccgcc ctggccgccc catccgcggc ctcggccgcc
     1981 gagcagctcg cggcggtcac caactccaac aaggtgctga cgttcgcctc ggactccccg
     2041 ggcaacctcg acaccgtcta ccccctgacg ggcctcgcct cgggcgagaa gatcgtcggc
     2101 ctggacacgc ggcccgccac cggccagctg tacggcctgg gcaagaccgg ccgcatctac
     2161 gtgatcgacg ccgcctcggg cgccacccgc tccgtcgccg ggcccatcag cccgcttccg
     2221 gccggctcga cctacggctt caacttcaac ccgaccgtgg accggatccg gatcaccacc
     2281 gaccagcgcc agaacctgcg cgtgaacccg gacaccggcg acacgatcgt ggacaccccg
     2341 ctggcctacg cggccggaca gggtggcggc gtggccccgc aggtgggcgg cgtggcctac
     2401 accaacccgg tgccgaccgc gaccaccacc cagctgttcg acctggaggc ggcgcgtgac
     2461 gcgctgacca cccagaaccc ggccaactcg ggcaccctga ccacggtcgg caacggcctg
     2521 ggccaggcca tctccggccc ctcgggcttc gacatcagca ccagcaacac cggctgggcg
     2581 tcgtacaagc cgggcggcca gggctcggtc agcctgttca agctcaacct gaccggcggc
     2641 acggtggcca acgccgcgtc gcagccgagc atcggcacgc gtgccgcggc ggacgacacg
     2701 gtggccatcg ccaccctggg cgccgtggcg gacgacaaga cggcgccgaa gatggtggcc
     2761 gactcgccga ccagcgccag ccgcgtgaac ctgtccaggg gcaagtcctt cctgggcacc
     2821 gtcagcctga acgaggccgg ccgggtccag gtggacatgc gcgtgggcaa gaccctggtg
     2881 gcccagggca aggccgagat ctacacccgg gccggctcca gccggatccg cgtcaaggcc
     2941 accacgtcgg gcatgaacac cctgaagcgc aagtacaaga ccgccacgtt ccgcttcagc
     3001 gcgcgtgacc gtgccggcaa cctgaccaag ggctcggccc gcaggttcac cctgcgctag
     3061 cagcccggag cgaaccccga ggggcgggcg tcagacctcc aggaggtcgg tccgcccctc
     3121 gcgctccagc aggtcgcggt agagcgggaa gaccggcttg atcagcggca ccagcttcag
     3181 gatcttggcc accgggcccg aggccttcat ctgcccgcgg gccagggcca cggtcacgtt
     3241 gacccggccc agccagaaca ggtgggccac gtcggcgtcg gcggtcatca cgatctcggg
     3301 ctcgaggtcg gactcgccca ggtcgacccg cacgtcctgg ccttcgatga gctggaaggt
     3361 gatcacggcc tcggggtcgt ggtagcggaa ctggacggtg gtgtcggcgc gcttgagctt
     3421 gtcgcccagg tccggagagt cgatcaggcc ggtcagcagg ccaccgatgt accggtagac
     3481 ctcgtcaccg tcgtcgaagt acgtcacctg gcgctgcggc cggaggcggc ggccatcgtc
     3541 gggtcgtgcg cggcgtcgcc cagcgcgtgc agctcctcct gcgcgaactc caccagccgc
     3601 tccaggtcgg gcatggtgtc gtagtcgccc atcagggaca gggcggccga gccgttgtag
     3661 ctcatcatcg cgatggccag gcgctgctcc ggcgccagga aggcgaccgg gatcagctcc
     3721 gccatctcgc gccccagcag gtagagcggg aactgcgggc cgggcacgtt cgtggtcagc
     3781 aggttgtaca tccgggtcga gaagtgcagc cgcgacgcct gggcgaagat cgtgggcggc
     3841 gcgaagtcct ccacgctggc gatcacctcg gcgcccatgg cctgcttgga ctccttgagg
     3901 tcggccatcg attccaccac gagccggtgg cgctccaccg ggtcctcgac gtaggtgggc
     3961 aggcgcccga tcatgatcgt gatctggttg cccaggtcgc cctcggcccc cgtggcgcgg
     4021 atggagaccg ggaccgcgga gcgcagctcc aggccctcgg tcacgacccc gcgggtgcgc
     4081 agccagcggt gcagcgcacc cgagaccacc gtcaggaaca cgtcgttcac ggtgcccccg
     4141 agctcgcgct tgatctcctt gagctcgtcg agcggcaggc gcgtccaggt c
//
